LOCUS       DAWTHD010000001        46701 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWTHD010000001 DAWTHD010000000
VERSION     DAWTHD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46701
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.001"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(25..363)
                     /locus_tag="WM704_000001"
     CDS             complement(25..363)
                     /locus_tag="WM704_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9339534.1"
                     /translation="MIYKNKIKEDGQDERLKEKAAQYRLFENNDTFQTALKPKFTFID
                     LFAGIGGFRIAMQNLGGEYVFSSEWDEKAKLTYEANFGEVPFGDITLEEIKQYIPKQF
                     DVLCGFTLSK"
     gene            complement(365..778)
                     /locus_tag="WM704_000002"
     CDS             complement(365..778)
                     /locus_tag="WM704_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFA9339535.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(795..1784)
                     /gene="pheS"
                     /locus_tag="WM704_000003"
     CDS             complement(795..1784)
                     /gene="pheS"
                     /locus_tag="WM704_000003"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFA9339536.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(2025..2384)
                     /gene="rplT"
                     /locus_tag="WM704_000004"
     CDS             complement(2025..2384)
                     /gene="rplT"
                     /locus_tag="WM704_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFA9339537.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(2397..2594)
                     /gene="rpmI"
                     /locus_tag="WM704_000005"
     CDS             complement(2397..2594)
                     /gene="rpmI"
                     /locus_tag="WM704_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFA9339538.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(2741..3262)
                     /gene="infC"
                     /locus_tag="WM704_000006"
     CDS             complement(2741..3262)
                     /gene="infC"
                     /locus_tag="WM704_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFA9339539.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(3280..5193)
                     /gene="thrS"
                     /locus_tag="WM704_000007"
     CDS             complement(3280..5193)
                     /gene="thrS"
                     /locus_tag="WM704_000007"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFA9339540.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGIYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(5246..5322)
                     /locus_tag="WM704_000008"
     tRNA            complement(5246..5322)
                     /locus_tag="WM704_000008"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5286..5288),aa:Val,seq:gac)
     gene            5501..6616
                     /gene="tgt"
                     /locus_tag="WM704_000009"
     CDS             5501..6616
                     /gene="tgt"
                     /locus_tag="WM704_000009"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFA9339541.1"
                     /translation="MLKFTLHKKDGLSRRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGVD"
     gene            6626..7708
                     /locus_tag="WM704_000010"
     CDS             6626..7708
                     /locus_tag="WM704_000010"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFA9339542.1"
                     /translation="MPSEAASDGILPPYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            7899..8367
                     /locus_tag="WM704_000011"
                     /pseudo
     CDS             7899..8367
                     /locus_tag="WM704_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
     gene            complement(8364..10094)
                     /locus_tag="WM704_000012"
     CDS             complement(8364..10094)
                     /locus_tag="WM704_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFA9339543.1"
                     /translation="MDWMNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAG
                     EEGVGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATV
                     LPLGLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGA
                     NDPMAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNL
                     AEGLYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAW
                     LAQATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAY
                     ISWLGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVA
                     MPNKPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIR
                     NGSRIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAA
                     RSGDLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGGGN
                     IGSMGLKVPR"
     gene            10289..11029
                     /locus_tag="WM704_000013"
                     /pseudo
     CDS             10289..11029
                     /locus_tag="WM704_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            11098..12636
                     /gene="lnt"
                     /locus_tag="WM704_000014"
     CDS             11098..12636
                     /gene="lnt"
                     /locus_tag="WM704_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFA9339544.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            12900..13763
                     /gene="rpoH"
                     /locus_tag="WM704_000015"
     CDS             12900..13763
                     /gene="rpoH"
                     /locus_tag="WM704_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFA9339545.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(13920..14396)
                     /locus_tag="WM704_000016"
     CDS             complement(13920..14396)
                     /locus_tag="WM704_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFA9339546.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKHLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSICSKHLSIPPICRKSS"
     gene            14496..15380
                     /locus_tag="WM704_000017"
     CDS             14496..15380
                     /locus_tag="WM704_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HFA9339547.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(15471..15986)
                     /locus_tag="WM704_000018"
     CDS             complement(15471..15986)
                     /locus_tag="WM704_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFA9339548.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(16062..16481)
                     /locus_tag="WM704_000019"
     CDS             complement(16062..16481)
                     /locus_tag="WM704_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339549.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(16602..17600)
                     /locus_tag="WM704_000020"
     CDS             complement(16602..17600)
                     /locus_tag="WM704_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFA9339550.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(17602..18081)
                     /locus_tag="WM704_000021"
     CDS             complement(17602..18081)
                     /locus_tag="WM704_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339551.1"
                     /translation="MNKIFLTAAALMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(18133..18912)
                     /gene="pgeF"
                     /locus_tag="WM704_000022"
     CDS             complement(18133..18912)
                     /gene="pgeF"
                     /locus_tag="WM704_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFA9339552.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            19098..19289
                     /locus_tag="WM704_000023"
     CDS             19098..19289
                     /locus_tag="WM704_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339553.1"
                     /translation="MLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGFNRKGMGIKSIS
                     DTSRAMPSENQSPLSDGIV"
     gene            19366..20313
                     /locus_tag="WM704_000024"
                     /pseudo
     CDS             19366..20313
                     /locus_tag="WM704_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(20466..21590)
                     /gene="rluD"
                     /locus_tag="WM704_000025"
     CDS             complement(20466..21590)
                     /gene="rluD"
                     /locus_tag="WM704_000025"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFA9339554.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            21589..22392
                     /locus_tag="WM704_000026"
     CDS             21589..22392
                     /locus_tag="WM704_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFA9339555.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(22462..24681)
                     /locus_tag="WM704_000027"
     CDS             complement(22462..24681)
                     /locus_tag="WM704_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFA9339556.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            24713..24988
                     /locus_tag="WM704_000028"
     CDS             24713..24988
                     /locus_tag="WM704_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339557.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            25067..29848
                     /locus_tag="WM704_000029"
     CDS             25067..29848
                     /locus_tag="WM704_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFA9339558.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKRGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGSSVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQDIVVAPPSPQANQAEEAKR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEERRQTAQSQPQRRKRRAAPQDYMAVSQ
                     DRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAEREAQALAARRK
                     AEAEEAKHQAAELAHRQEAKRKAAESAKRKAEEEEHRQTAQSQPQRRKRRAAPQDYMA
                     VSQDRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEDVS
                     ESVDTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALSDAMAST
                     QSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGI
                     DRSLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSL
                     RSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVK
                     VSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAYKADNQQ
                     QYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(29924..31126)
                     /gene="trpB"
                     /locus_tag="WM704_000030"
     CDS             complement(29924..31126)
                     /gene="trpB"
                     /locus_tag="WM704_000030"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFA9339559.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(31209..31838)
                     /locus_tag="WM704_000031"
     CDS             complement(31209..31838)
                     /locus_tag="WM704_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFA9339560.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            32114..32893
                     /gene="rsmA"
                     /locus_tag="WM704_000032"
     CDS             32114..32893
                     /gene="rsmA"
                     /locus_tag="WM704_000032"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFA9339561.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            32908..33477
                     /locus_tag="WM704_000033"
     CDS             32908..33477
                     /locus_tag="WM704_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFA9339562.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            33588..34043
                     /locus_tag="WM704_000034"
     CDS             33588..34043
                     /locus_tag="WM704_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339563.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            34085..34813
                     /locus_tag="WM704_000035"
     CDS             34085..34813
                     /locus_tag="WM704_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9339564.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            34886..35245
                     /locus_tag="WM704_000036"
     CDS             34886..35245
                     /locus_tag="WM704_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339565.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            35250..35690
                     /locus_tag="WM704_000037"
     CDS             35250..35690
                     /locus_tag="WM704_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HFA9339566.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKRRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            35721..36995
                     /gene="folC"
                     /locus_tag="WM704_000038"
     CDS             35721..36995
                     /gene="folC"
                     /locus_tag="WM704_000038"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFA9339567.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            37009..38049
                     /gene="ftsN"
                     /locus_tag="WM704_000039"
     CDS             37009..38049
                     /gene="ftsN"
                     /locus_tag="WM704_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFA9339568.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            38042..38539
                     /locus_tag="WM704_000040"
     CDS             38042..38539
                     /locus_tag="WM704_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFA9339569.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            38733..40277
                     /gene="purF"
                     /locus_tag="WM704_000041"
     CDS             38733..40277
                     /gene="purF"
                     /locus_tag="WM704_000041"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFA9339570.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            40377..40868
                     /gene="greB"
                     /locus_tag="WM704_000042"
     CDS             40377..40868
                     /gene="greB"
                     /locus_tag="WM704_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFA9339571.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            40927..41553
                     /locus_tag="WM704_000043"
     CDS             40927..41553
                     /locus_tag="WM704_000043"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFA9339572.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFINESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(42027..44858)
                     /gene="lbpA"
                     /locus_tag="WM704_000044"
                     /gene_synonym="tbpA"
     CDS             complement(42027..44858)
                     /gene="lbpA"
                     /locus_tag="WM704_000044"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFA9339573.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(44855..46434)
                     /locus_tag="WM704_000045"
                     /pseudo
     CDS             complement(44855..46434)
                     /locus_tag="WM704_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(46486..46701)
                     /locus_tag="WM704_000046"
                     /pseudo
     CDS             complement(46486..46701)
                     /locus_tag="WM704_000046"
                     /inference="COORDINATES: protein motif:HMM:NF036352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
BASE COUNT        11767 a        13188 c        11291 g        10455 t
ORIGIN      
        1 gaaagcctca tatttctaaa atctttattt tgataaggta aacccacaca gcacatcaaa
       61 ttgcttcgga atgtattgtt tgatttcttc caaagtaata tcgccaaacg gcacttctcc
      121 gaaattggct tcgtacgtca gtttggcttt ttcgtcccac tcgctcgaaa acacgtattc
      181 cccgcccaag ttctgcatcg cgatgcggaa gcccccgata cctgcgaaca agtcgataaa
      241 ggtaaatttg ggtttcaagg ccgtctgaaa agtatcgttg ttttcaaaaa ggcggtattg
      301 tgctgctttt tcttttaacc gttcatcctg cccatcttcc ttaatcttgt ttttgtaaat
      361 caattcatct tgtttggatg atttctttga ctttttcggc acagcaggtt gtatttttga
      421 cgacatcgtt actccaaaaa cgtaaaaccg tccagccctc ggctttcaga cggcctgtta
      481 cttctatatc cctttggata ttgcgctcaa ttttggcaat ccaaaactca cgatttccct
      541 ttattaccgc ttttttctgt tcccaatcct taccgtgcca gaattcccca tcgacaaata
      601 cggcaacttt atatttttta aatgaaaaat caggttttcc aaaaatgctt ccgctatttt
      661 tccgatacct gagccccaaa gcccacattg ccttcgccaa cacaagctcg ggtttcgtcc
      721 ctgtactttt gttggactgc atacattttt tgcgctgctc cggggttaat ttatccattg
      781 ggtttatttg agatttattc aaactgcttc aagaagttca aatcgttatc gaagaacaag
      841 cgcaggtcat tgacgttgta gcgcaacatg gcaaagcggt cgagaccaat accgaaagcg
      901 aaaccggtat atttttcagg gtcgatattg acatttttca acacattagg atgtaccata
      961 ccgcaaccgc ctacttccaa ccatttgccg ttttctccca tgatgtcgat ttcggcagaa
     1021 ggctcggtaa acgggaagaa agacggacgg aaacgcactt gtaggtcatc gcgttcaaag
     1081 aagcgacgga taaagtcagt gaacactgct tttaagtcgg caaaagttac gccctcttct
     1141 acccacaaac cctcggcctg atggaacata ggcgagtgcg tggcatcgct gtccacacgg
     1201 taaacgcggc cgggggcgat aatgcggatg ggcggctctt ttttatcgag catatagcgg
     1261 atttgaatcg gggaagtgtg cgtacgcaaa acatcgccgt tttcaacgta aaacgtatcc
     1321 tgcatcgcac gggcaggatg gtttgcaggg atgttcaggg cttggaaatt gtgaaaatcg
     1381 tcttcgattt caggcccgtc cgccacttcg aaacccattc cgtgaaagag ttcgaccaca
     1441 cgttgcaagg tcagggttac gggatgcagg ctgccgcctt cctgagcgcg tccgggcagg
     1501 gtaatgtcga gggcttcggc ggcaagtcgg gcttgcagct tggcttcgtt gagggcatcg
     1561 cgtttggcat taaaagccgt ctgaaaccgg tttttgcatt cattgatatg cgcacctatg
     1621 gttttgcgct cttcaggcga catttgcccc aaagttttca gaagtccggt caactcgccg
     1681 gttttgccaa gataacgggc tttgatttgt tctagagcgt tgaagtcttg cgcagcttct
     1741 actgcggcaa tgccttctgc aacgatacgg tttacatttt ccataatatc aagcctgtca
     1801 attaatgata tgccacctga aacaacgagg ccgcctgtat agcctgacat atcagactct
     1861 tcagacgacc ttattttctt cacggccaat ccattggata aattttctgt attttaacgc
     1921 aaatttattt aaaaaaatag actttaaaga aagaaaatca atcacataga atttccgcaa
     1981 acaaagaaaa aaggaagccg cagcttcctt ttcaattttt tggattaagc agccaaagca
     2041 gctttggctt tttcaaccaa ttgtgcaaaa gcggctttat cgaacacggc caaatcagcc
     2101 aatactttgc ggtcgatttc gatagaggcg cgtttcagac cgttcataaa tttgctgtaa
     2161 gacaacccgt tttcacgcgc acctgcattg atacgaacaa tccacaattg acggaattgg
     2221 cgtttgcgtt ggcggcggtc acggtatgcg tattgaccag ctttcattac cgcctgcttg
     2281 gcaacgcggt aaacgttttt acgacgacca cgatagcctt tggctaacgc gaagattttt
     2341 tggtgacggg cacgggcggt aacaccgcgt tttacgcgtg gcatattcta aactccttaa
     2401 gcgtagggta acattttagc aacagaagcc aaatcgcgat catttaccat agaggtaccg
     2461 cgcagttggc gtttgttttt ggtggtcttt ttagtcaaga tgtggcgttt gaacgcatga
     2521 gcgcgtttca caccgccgtt acccagtact ttaaagcgtt ttttcgcgct agacttggtt
     2581 ttcattttag gcatgggaaa actccattcg ttatcggata aggcattagg gggttttaaa
     2641 acatcggttt caaacacttg aacccacaat gccacggttt tttgccgcaa ttgaaaactt
     2701 actccgaagc ggcaatcgga gtaagcggaa aattatagct ttattttttc ttcggcgcaa
     2761 tcatcatcac catttgacgg ccttccattt tgggaaagga ctcgatttgc gccacttcag
     2821 ccaaatcttc ttttacacgt tccaaaagtt gcgcgccgag ttgctggtga gccatttcac
     2881 ggccgcggaa acgcaatgtc actttgactt tatcgccgtc ggcaaggaag cggttgatgt
     2941 tgcgcatctt gatttgataa tcgccctcat ctgtacccgg acggaatttg atttccttga
     3001 tttgcacctg cttttggttt ttcttggctt cgtcgcgctt cttggcttgt tggtatttgt
     3061 atttaccgta atccatcagt ttgcacacag gaggtttagc agttggggaa atctctacca
     3121 aatcgacatc ctgcccttcg gccatagcca aagcttcacg aactgaaacg acaccaagct
     3181 gttcgcctga ctcactgatt aaacgcactt ctttggcggt aatttcaccg ttgattcgtg
     3241 cttcgcgttc ttgagcgatg atgaatactc ctataaaaat taatgattga cgagggcatc
     3301 agtgatttct tgctgcaatt gcgcaatgaa atcatccaaa tccaaagaac ccaagtcttc
     3361 cgctttgcgg cgtaccgcaa ctttgttttc ttgtttctct ttatcgccga taacgatttg
     3421 ataagggaaa cggtattggc tgttgtcgcg gattttgtaa ccgatttttt cattacgcaa
     3481 atccaactcg gcgcggaagc ctgccgcctg caatttggca gcaacttccc gacaataatc
     3541 tgcctgattt tcggtgatgt tcataatcac catttgaacc ggtgcaagcc ataacgggaa
     3601 agagcctgca tggttctcaa tcagaatgcc gacaaaccgc tccaaagaac ctaaaatggc
     3661 gcgatgcaac ataacaggcc gcgctcggtc gttgttttct gttacatatt ctgcatccaa
     3721 gcgttccggc aagacgaaat ccagttgtaa tgtaccgcat tgccaagaac gacccaaggc
     3781 atctttgacg tgatattcga ttttaggccc gtaaaacgca ccctcgcccg gcaattcgcc
     3841 ccattccacg ccgcaggcag tcaatgcctc gcgcaaaccc tgctctgcct tatcccacac
     3901 gtcgtctgaa cctgcgcgtt tttcagggcg aagggaaagc ctgacggata catcatggaa
     3961 accgaactgt ttgtagatgc gaaccaacaa ttcattgaac gcacgagcct cgctgacgat
     4021 ttgatcttcg gtacaaaaaa tatgcgcatc atcctgcaca aaaccgcgaa ccctcatcag
     4081 accgtgcagt gcgccgctcg gctcgttgcg gtggcaagaa ccgaattccg ccaaacgcat
     4141 cggcaaatct cgatatgaac gcaaaccgtt gttgaaaatt tgaacatgac ccggacagtt
     4201 catcggttta accgcatatt cgcgtttttc cgaactggtt acgaacatat tgtctttgta
     4261 gttgtcccag tggccggatt tttcccaaaa ggttttatcc ataatttgag gcgttttgac
     4321 ctctttataa ccggcggcgt tcagctcttt acgcatatgc tgctcgatag tctgccacaa
     4381 agcccaacct ttgggatgcc aaaacaccat acccggcgct tcgtcctgca agtggaacaa
     4441 atccagttgc ttgcccaatt tgcggtggtc acgtttttct gcttcttcaa tgcgttggat
     4501 atagtctttt aattcgtctt tcgtcgccca agcagtgcca taaatacgtt gcagcatttc
     4561 attattgctg tcgccgcgcc aatatgcgcc tgccagcttg gtcagcttga agtttttcag
     4621 gaagcgggtg ttcggaacgt gcggaccgcg gcacatatcg acatattcct ggtgatgata
     4681 tatccccatc gcttccactt cgggcatatc ttcaatcagg cgcagtttgt attcttcgcc
     4741 gcgctcttga aaaattttaa tcgcctccgc acgcggagtc atgattttga ccacatcata
     4801 atcttgggca atcaattctt tcatacgcgc ttcaatggcg gcaacatctt ccggtgtaaa
     4861 cggtttttcc gtggcgatgt cgtaataaaa gccctcttca atgacggggc cgataaccat
     4921 ttttgcatta ggatagagtt gcttgacggc atgcccgaca agatgtgcgc aggaatggcg
     4981 gatgatttcg atgccttcct gatctttcgg agtaatgatt tgaacagcag aatcctcaac
     5041 aatcgggtcg cacgcatcga ccaatttgcc gtttaccttg cctgccaccg ccgccttcgc
     5101 caaaccggca ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg
     5161 gactgaacag tccggcaagg taatattcaa catcaaacgc tccgaaagat taaaaaataa
     5221 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     5281 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgtatt
     5341 ttatcgggtc gttttaattt ttgcaagaga gatgtgcatt tctttatgca acattgtggc
     5401 aagatttggg gataccgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     5461 atatatttcg acccctgtcg cccaacttac attcaatctt atgcttaaat ttactttaca
     5521 caaaaaagac ggtctctccc gacgcggcac gctggagttg aaccacggaa aaatcgaaac
     5581 gccggttttc atgccggtcg gtacttacgg ttcggtcaaa gcgatgaacc cgcaaaacct
     5641 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     5701 tttggaggtc gtcgaacagt ttggagggct tcacggtttt atcggctggg acaaaccgat
     5761 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgaccga
     5821 agaaggctgt acgtttaaaa gcccgattaa cggcgacaag ctgtttttat cacctgaaat
     5881 ttccatgaaa atccaaacgg ttttgaactc ggatatcgcg atgcagttgg acgaatgcac
     5941 gccgggcgaa acaacgcgcg aacaggcgcg aaaatccctg caaatgagct tgcgctgggc
     6001 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcgctgt tcggcatcgt
     6061 gcaaggcgcg atgtatgagg atttgcgcga agaatctttg cgcggtttgg aggaatttga
     6121 ttttcccggc cttgccgtcg gcggtttgtc cgtcggcgaa cccaagcccg aaatgtaccg
     6181 tatgctgcac gccgtcggcc cgattttgcc ggaacacaag ccgcattacc tgatgggcgt
     6241 aggcacgccg gaagacctcg tgtacggcgt ggcgcacggt atcgatatgt tcgactgcgt
     6301 gatgcccacc cgcaatgcgc gcaacggctg gctgtttacc cgtttcggcg atttgaaaat
     6361 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
     6421 ctgccaaaat ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
     6481 cgcgcaattg aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg
     6541 cgaagccgtc gaacaaggca aatttgccga ctggcaggcg caattccacg aaaaccgtgc
     6601 gcgcggcgtg gactgattcc cggcaatgcc gtctgaagcc gcttcagacg gcattttgcc
     6661 gccttaccct acctgcacta tttttcagaa accgatcatg cttccattcc ttccagaacc
     6721 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacacc
     6781 cgacgcaccg accgcacagg cggtacgccc ttatctgaaa tcctttctga ccgaccggcg
     6841 catcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggcctgg tactgacgtt
     6901 ccgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgcccct
     6961 agaagtttac acagcacgcc aagctgccgc gcttgccgaa cgtatgcccg acctcatcgt
     7021 ccgccacgcc atgacttacg gcaatccttc gatagcggat gtattggcgg agctgaaatc
     7081 ccaaggtgta ggcagattgc ttgcgattcc cctatatccg caatatgccg cttccagcag
     7141 cggagcggcg gtagataaag tgtgcgaaca actgctgctg caacgcaacc aaatgagcgt
     7201 ccgcaccatt tcgcgctttt atgatgatgc cggctacatt gatgcgatga aaaaccacat
     7261 cctccgatat tgggcggaac acgggcgtgg gaaaaaactg atgttgagtt ttcacggcgt
     7321 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
     7381 actgcttgcc gaagcactgg aactgaccga agacgaatat accgtatcgt tccaaagcca
     7441 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
     7501 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gcttggaaac
     7561 catggaagaa atcgccctga cggggcggga acagttttat gaagcgggcg gaaaaaacta
     7621 ccgctacatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
     7681 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgagc catgtctaaa atgccgtctg
     7741 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgccgga
     7801 cagcacatca agtgcaacct tactaccgat tcggcaaaaa aacctttcac ccgctacgca
     7861 aaatcaacaa caaggtttca aacattaagg aaacgccaat gaacacaacc cgactgccga
     7921 ccgccctcgt cttgcacttc ctctgcgccg ccgcttctgc cgccgacaac agcatcatga
     7981 caaaagggca aaaagtgtac gaatccaact gcgtcgcctg ccacggcaaa aagggcgaag
     8041 gccgcggaac catgtttccg ccgctctacc gctccgactt catcatgaaa aaaccgcacg
     8101 tcctgctgca cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct
     8161 acaacggatt catgcccgca accgccatca gcgatgcgga cattgccgcc gtcgccactt
     8221 atatcatgaa cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg
     8281 caaaaggcaa aaaaaaacca aacagacaaa atgccgtccg aaaccggcaa tccggcttca
     8341 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
     8401 ccgccaccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggtcgccc
     8461 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
     8521 tcgcccgctt ccagcttcaa accgtaagca agtgccaaat cgcccgaacg tgctgccggc
     8581 gaaacgacaa actcaccgaa gaaatcaaaa ttttcacgga cgccgatacc cgtctcggta
     8641 aagtatttcg ccattttatc gaccttgccg tcgggcagga tgtaccacgc caaatcctcc
     8701 gcctgaagca cggtgtcgga ctgcatttcg attcggctgc cgttgcgtat caatgcgaaa
     8761 cagcgtgcgt caaacgaatc ggaaatcggt tcgaccgtat cgggatggtg tccctccgct
     8821 tcagattcgg caaccacttt aaacgccgac attctgacaa tttccttttc cgccagccaa
     8881 atatcatgcg tatctttcgg ttcgggcttg ttgggcatgg caaccttcaa cagccgcgcc
     8941 atcacaggaa tcgtcgttcc ctgaatcagc agcgacagca ccaccacggc aaacgccaca
     9001 tcaaacagca ggtgcgaatt gggaacgccc atcaccagcg gcatcatcgc cagcgaaatc
     9061 ggtacggctc ctcgcaagcc caaccaactg atatacgcct tttcacgcag gctgtaattg
     9121 aatttccaca aaccgccgaa cactgccagc ggacgcgcga ccagcatcag gaacgccgca
     9181 atcgccaagg cttccgccgc cctgtccaac acgccggcgg gagaaaccag cagaccgagc
     9241 atgacgaaca aagttgcctg cgccagccaa gccaaaccgt ccatcacacg caaaacgtgt
     9301 tccgtcgcac ggttgcgctg gttaccgaca atgatgccgg caaggtaaac cgccaaaaag
     9361 ccgctgccgc ctatggtatt ggtaaacgca aacacaagca gcccgcccga cacaatcatc
     9421 agcgcgtaca gaccttccgc aagattcaga cggcgtacca gctttgccaa tatctttccg
     9481 cccgcccaac ccgtcagcag accgaaaccg atttgcagcg caagcatccg gacaaacgct
     9541 gccgcacccg attccgccgg ctgcataatc atggtaatca gtgccgtaac caaaaaaacc
     9601 gccatcgggt cgttcgcacc cgattcgatt tccaaagtcg cctgcacccg ttcgttcaaa
     9661 cgcacgccgc tgttgcgcaa aaggctgaat accgcgccgg catcggtcga accgacaatc
     9721 gccgccatca gcacgccgaa cttccaatcc aaaccgagat aaaaagttgc aaacagtccc
     9781 agcggaagca cggtggcaaa cacgccccac gaagcgagta ccgacgcggg cttcaacgca
     9841 atccgaaaac tggaaagctg cgtccgcaaa ccgccgtcga gcagaataac cgccaaagca
     9901 agctggctga tgaaattcgc catcacgaca ttattgaagg caatgccgcc aacgccttcc
     9961 tcgcccgcca gcatgccgac ggcaagaaac accagcagca aaggcattcc caaacgtgcg
    10021 gacaaagtgg tcgaaaccac gctcaggaac aacagtgcgc cacccagcag aaacaggctg
    10081 ttcatccaat ccacgccaac ggctccccct taaaattcat gaaattagaa tcgaaagatt
    10141 gtaacacggc attttcttct cacgaaactt tatttcatgt ccgccaccgc cttcagacgg
    10201 cattttcctg tttcatgccg cctgataccg cccaaacccg cgttctccta tataatgtcg
    10261 gactttgcgc cgcatttcag gattttttat ggcatttgcc tcactattta ctctgccgga
    10321 ctacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg caaagaaaac
    10381 cgccggcgtg gccggcgacg atttggcacc caatgccaac cgggttacag gcgtatcggc
    10441 agaacgcgag ttgccgattg tttggccggt ggcgaaaggt tcttttgcca acaagccggt
    10501 tttggtgtct gcggctctgc tgctttccgc cttcctgccg caactcatta cgccgctttt
    10561 gacggcaggc gggatttatt tgtgctttga gggcgtggaa aagctgctgc acaagtttct
    10621 gcaccgacat gacgcgcacg atgacggcgg cgaaacggcg gttaacgagc aggctaaaat
    10681 caaaggtgca atccgtaccg actttatcct gtccgcagaa atcatcatca tcgcgctgtg
    10741 cgtggtcgaa caatacagcc tgatgacgcg ctcgctcgtg atggcggcaa tcggcatcgg
    10801 catgactgtt ttggtgtacg gaatcgtcgc cgtcatcgtc aaactcgacg acttgggcat
    10861 gctgctgatg cgccgcccac aacctcggcc ttttgcacga tttcctgcac gcgcaacact
    10921 ggaacgctgg ctggacagag tattccgcca acctcgttgt cgggccgctt tccggttcga
    10981 ttgcctgcgc cccgccttgt cgctgatgaa tcgtttcggc aggcattgat tcctttttca
    11041 aataccgatg ccgtctgaaa gacgttcaga cggcatattc cgaacagaca gacgaatatg
    11101 ttttccaaac tggacaaata ctggcagcac cccgccctct actggccttt gctcatcctt
    11161 tttgccgccg ccaccccctt cgccttcgca ccctactacc acttttggct gatgcccttg
    11221 attttcggcg ccttcgtccg cctcatcgaa ctgcgtccgc gttttgccgc ctcttccgcc
    11281 tacctgttcg gtctgaccgc atacacggca cagttctact ggatacacac tgccctgcac
    11341 gacgtttccg gcctgcccga cctctatgcc gtaccgctga ccttcctgct gcccgcctac
    11401 cttgcccttt atccggcact gtgtttctgg ctgtggaaaa aatttaccct gccccagggc
    11461 ataaagaccg gtctggtact gcccatcctg tggacgctga ccgagtttgc ccgcgagcgt
    11521 ttcctgaccg gattcggctg gggcgcaatc ggctactccc aaatcacccc ggacagcccg
    11581 ctggcgggtt tcgccccctt gggcggcatc cacatggtta cactggcaac cgcctttctc
    11641 ggtgtctggc tggttttggc gatcgacaac accgcacgtt cgggcaaacg cctgcttccg
    11701 gccattctga ttgccgccct gcttgccgca ggctacaccg cccaacaaac cgacttcacc
    11761 cgccccgacg gcagccgcag caccgtcgcc ctgcttcagg gcaacatcgc ccaaaccctc
    11821 aaatggcgtg aagaccaagt catcccgacc atacagaaat attacgaaca ggtcggcaaa
    11881 accactgccg acatcgtcat cctgcccgaa accgccattc ccatcatgcg ccaaaacctg
    11941 ccggaaaaca tactggcgca attcgccgaa caggcgcaaa acaacggcag cgcgctcgcc
    12001 gtcggcatca gccaatacac ttcggacggc aacggttacg aaaacgccgt catcaacctg
    12061 accggctatc aggaaaacaa tcaggacggc atcccttact acgccaaaaa ccacctcgtc
    12121 cccttcggcg aatacaaacc gctgcccctc ctgaccacac cgctttacaa aatgatggac
    12181 atgccccttt ccgacttccg caaaggcggc ggcaagcaat ccgccatgat gatgaaaaac
    12241 caaaaaatcg ccttcaacat ctgctacgaa gacggattcg gcgacgaact gattgccgcc
    12301 gccaaagacg ccacactgct tgccaatgtc agcaatatgg cgtggtatgg aaaatccaac
    12361 gccatgtacc agcacctcca acaatcgcag gcgcgggcta tggaactcgg acgctatatg
    12421 gtccgcgcca ccaacaccgg cgcaaccgcc atcatctccc ccaaaggcaa catcatcgcc
    12481 caagcccaac ccgatacgga aaccgtatta gaaggacaca tcaaaggcta catcggcgaa
    12541 acgccctata tgaaaaccgg cagttcatgg tggttgatgg gcatattgac cctagccgca
    12601 ctgattcttt tcatcttccg aaacaaagaa cactgatacc tgcacagtcc gtccggacgg
    12661 gctgtctttt ttcaaaccgg ccgtttacat acaaccacgt atgtaatata attctaaaat
    12721 cgaattgagc tatcaggttt acaaaatcaa tcaaacttgc cttacaaaat tttcctgtct
    12781 ttcaaacctt agcgtatatc tcctgccaaa catacaaaat acaagccaag cagtgatata
    12841 ctgcatccca acacaccaaa ccgccgatac gcagtttcag ccgaaaggaa atgccccaaa
    12901 tgaataacgc tttcgcatta cccgccatcc aaagcggcaa cggcagcctc gaacaataca
    12961 tccacaccgt caacagcatc ccgatgctgt cccaggaaga agaaacccgc ctcgccgaac
    13021 gccggataaa gggcgacctc aacgccgcca aacaactcat cctgtcccac ctgcgcgtcg
    13081 tcgtttccat cgcgcgcggc tatgacggct acgggctgaa tcaggccgac ctgattcagg
    13141 aaggaaatat cggtctgatg aaggcggtca aacgctacga gcccggtcga ggtgcacgcc
    13201 tgttttcatt tgccgtgcat tggattaaag cggaaattca cgaattcatc ctgaggaact
    13261 ggcgccttgt ccgcgttgcc accaccaaac cgcaacgcaa gctgtttttc aacctgcgca
    13321 gcatgcgtaa aaaccttaat gtcctgtctc ccaaagaggc acaagacatc gccgacgatt
    13381 tgggtgtcaa actttccgaa gttctggaaa tggaacagcg catgacggga cacgacatcg
    13441 ccatcatggc agacaacagc gacgacgagg acagtttcgc ccccatcgac tggcttgccg
    13501 accacgattc cgaaccgagc cgacaactat cgaaacaggc gcattacgcc ctgcaaaccg
    13561 aaggcctgca aaacgccttg gcgcaattgg acgacaggag ccgccgcatt gtggaaagcc
    13621 gctggcttca agacgacggc ggactgacgc tgcaccagct tgccgccgaa tacggcgtat
    13681 ctgccgagcg catccgccag attgaagcga aagccatgca gaaactgcgc ggtttcctga
    13741 ccgaagaagc cgaagcggtt tgaaatatac acaataaaat acccgacaaa acaatttgtc
    13801 gggtatttta tttggtaaac atcaatgcgg tttgtgcgtc gtgccgtaca cgattttatc
    13861 cgcccaagcc atcgcaatcg ccaaaatcaa aaagcagaca tgcaccagac actgccacat
    13921 cagctgcttt tccgacagat tggcggaatt gataaatgtt tggagcaaat ggatggatga
    13981 ataccgataa tcgacatcga cagccttacc ttcaataccg gtgcattcac atggctcaac
    14041 cactcgggcc ggtcaggatg gtcgtcgata cgcaaccggg atacgaacga ctcatacccg
    14101 ccaatctgca ccatggtcag caaattcgca atcataacca catcaatcag atttaaaaca
    14161 gcgagcatga tggcgttttc gtccgacaca tccaaattca tgaccagatg cttcaacgat
    14221 ttcaaaaact tataggcaca aatcgcccgt acaacagtca gccccgcata aatcggcact
    14281 tgcagccaac ggcttgcaaa aatcattttg gcaaaaaacc catagcctgt tcgggttttg
    14341 gagattttgc ttgatacatt tcccttcctt gcagcaacaa aagcgcgtga ttccaatcta
    14401 ccgctgcgaa atcttcaatt tttcacaccc gaaaacgctt gccgccatac agatgtgata
    14461 tattgattcc attttaacga acttcggaga acgacatgag cagcggaaat atccgcatcc
    14521 acagcatgac cggctttgcc aacgcggcgg cagagtgcgg cagcaaacgc atcaacctcg
    14581 acatccgcgc cgtcaaccac cgctttttgg acattcaaat caggatgccc gacgatttgc
    14641 gccatttgga aaacggcatc cgcgagcaga tttcatccca tctggcgcgc ggcaaagtcg
    14701 aatgcaaaat ccaaattcaa gatgcggaaa acggcaatca gtctttggaa ctgaatcgcg
    14761 ttctggtcgg acagcttgcc gaaatcaaca aaaacctccg caaacattat gatttggcaa
    14821 aactgggcgt tgctgatatt ttgcgctttc cgggcgtatt ggcaagccaa aaggaaaata
    14881 cggaagcatt ggcgaaaacc attgtcgaac tggccgacaa agcactaaaa gactttaccg
    14941 ccgcacgtcg gcgtgaaggg aaaaaattgg gcgaacacct gttacaacgc attaaaaaca
    15001 tggaagaaat catcgatgct ttgagcgaaa tcttccccgc gctggtggaa gcgcataaag
    15061 aaaaaatccg cacccgcctt gccgaagccg tcggcagcat cgacaacgac aggctgcaac
    15121 aggaatttgc cctctttatc caaaaatccg acatcgacga agaattcagc cgcctgcgca
    15181 cccacattgc cgaagtgcgc cgtatcgtta ccgaacacaa aggcagcagc ggcaaacggt
    15241 tggacttcct gatgcaggaa ctgaaccgcg aagccaacac tttgggcagc aaatccatcg
    15301 ctaccgaatg cacccaagcc tcggtcgagc tgaaagtcct gatcgaacag atgcgcgaac
    15361 aggtgcagaa tatcgaataa ccctgccttt ctctccaaac atgacaatgc cgtctgaaac
    15421 ttttttcaga cggcattctt gatcggctga aaacctctgt tgcaagcaat tcaagattgt
    15481 tctttcacat ccaccaacct cgttccggca aggaagtcat acagaaactg ccggtcggga
    15541 ttcagcaggg cgaaccccca cggcaaaatc agccaaacca atgccgcgcc ggccgcgccc
    15601 ttgggcggaa tgccgaggaa gtggcgcaat ccggcataag caagcatagg aatgaagacg
    15661 atgaatatgc acgcccaaat aaagcgcagg cgcagcaggt gcaaagacgg ctgtatgccg
    15721 ttaaggtcgc acaagccgat tttccatgtc ctcatcgcca aggtctgccc ctgaccgtgc
    15781 cagttggcgc ggaaataaag ccaccatgct cccattatca ggatactcgt taccaatgca
    15841 gaaaccgcga tagaaacggg attcagaaaa atggcggcaa taccggcaag caatgctgcc
    15901 aaacaggtcg ccgcaccgac cagcagcatc tcatacagta tggcggcaaa gcggcgtttg
    15961 agcggggcgg ttttcagtat catcatcttt tcaatcggaa tatgccgtct gaaacgccaa
    16021 cagacttcag acggcatttg tgcaaagatg ccttgcggcg gttaacggtg cggacgtttc
    16081 tgaccgcgcg actgttttgc cgccatttga ttgatttcct gacgtgtcgg gcgttttttg
    16141 aacatagcgt gcgcctcgtc caccgaacat ttttgcgaca ggcagatcag ggtaatcgca
    16201 tctttctggt gttgtccgcc gctgtggtac atacggcgca ggtttgcctc gttgattctg
    16261 cctttttcgg gatcgatgtc gaattttttc agacgttcga taaactggcg ttgctgtcgg
    16321 cgggatgtcc aaacaaagcc gcccgcagcg gcggcgacga tgacggcggc aagcaggatg
    16381 gcaagcggcc aggaatcgac aatcaatacg ataatgaggc ttaaaacggc ggtggtcagt
    16441 aaaatcaggc cgcccaagcg cagtttggtc ttattgtcca tagggttgat gttcctttat
    16501 tgaggtggtc gtgccgtctg aaatgttttc ggacggattg gtgaatactt ttcaggatta
    16561 acggtatttt acagtatttt cggattttgg ggattaccgc tttattccgc cagcgacacg
    16621 acaagctgtt tgaataccgt ccatgcgtcg ccgtcttccg cgcctttgat gattcggtcg
    16681 atttgggcgc aggttttgag cgcgtcaagc aggcggacga cggaaatccg cttgaccgca
    16741 agcggtgcga gcgtctgttt gtcgccccaa agcctgaggc tgttgcggac ggattggatg
    16801 ctctgcccct gtttcagggc agcggcaagc cgaatcagcg tccgcacgtc ttcggcaacc
    16861 gcccacagaa gcagcaccgg ttcttcgcct tcttcctcca acccgtccaa cagcctgcat
    16921 acgcgcggga catcgccctt catccacgcg cctgccagtt ggaacgcgtc gaagcgggca
    16981 acattggcaa cggcggtttg cgcctcatcg atattgacgg catgcccttt cggatacagc
    17041 agcgcgagct tgtcgatttc ctgacgtgcc gccaacagat tgccttccac gcgctcggca
    17101 aacagtgcca atgcgtctgc ctcgataccc aaaccgattt tgtccagccg tccgcgtatc
    17161 cattggggca aagccgccgc accgaccggt ttggcttccc atacttcccc ctttgccgcc
    17221 aatgcggcaa accatttgga ctggagccgg gttttctcca gtttgggcag caaaaccagc
    17281 gttaccgtat cttccggcag tcgggcggca aaatcctgca atgcctcgcc gccgtttttg
    17341 ccgggttttc cgttggggat atgcaattcc aacagtttca aatcggcaaa cagacccgca
    17401 ctgcctgcgg tttgcagcag ttcgttccaa tcgaaagacg cgtcggcggt ataggcttcg
    17461 cggttgagat aaccctgctt cttcgccgcc gccctcaatg cgtccactgc ctcgatacgc
    17521 agcagttctt cctcgccgtg gatgacgtac agaggtttca aaggcgcgtc cgtatcgatg
    17581 cgtccgatat gtgccgccac gtcattccgc cttcagaaag gtcaggcggc ggacaatctg
    17641 ttcggcaaca tcctgccgca tttccgccca cagggtttct tcttcttcct gtttgcccaa
    17701 aatttcgttg tcggcataat ccaaaatgcg gcggacggac acggtcatcg gtttgccgac
    17761 cggctcgccg cgtttcaata cctgcgcttc aaccgtcaat atcaaaagat attcgttgat
    17821 gactgccgca cgggtaacgg tataggtttc cttgttttgg gaaacgctgt ctatacgcag
    17881 ggtcatctgc gcgccggcag catcgtccac cctgcccgaa gcctgataca gcgcggtttc
    17941 caaaggaaac tgcaatgcct gtccgccttc gatgtgccag ctccggtagg tcagcggcgg
    18001 agaaatgccg tctgcacctt tcaggtggaa accgcacgcg cccagcatca aggctgcggc
    18061 agtaaggaat attttgttca tcgcttgcct ttctcaaaag cataaagtcg gtagattata
    18121 tcagcggcgt gttcagacgg cattgccgtc cagccaaatc aggctcgcca tacgccccgt
    18181 cgctccgtcg cggcggtagg aaaagaaaat atcccgttcc aaaaccgtac aatgcgtgcc
    18241 gccgtacacg ccgcccacgc cttcgcgctt cagaatcagg cgggcgagcg cgtagaggtc
    18301 ggcaaggaat ttgccgccgc cgatgggttc aaatgcagca gcggcttcgg gcatggacgt
    18361 gcagaacgca tcaaacacgt cctgcccgac ttcaaacgca tccgcactga tggcagggcc
    18421 gagatacgcc ataatttcga caggcggaac cttcatcgcg gcaatggtgt tttgcaatac
    18481 gccgcccgcc aaaccgctcc agcccgcgtg tgcggcggca accgccgtac ctgccctgtc
    18541 gcaaaataga acgggcagac agtctgcagt catcacggca cagacggctt tacccgtgtc
    18601 gtccacggaa gcgtcggcat cgggcgtgcc gtccaatgct tcggcagcat tgacgacaat
    18661 ggtgctgtgg atttgattga gataggcaac gggcaatccg acctgctgtt gcacgatttc
    18721 gcggttgcgg cgcacggttt cagggtcgtc gccgacgtgc gtaccgaggt tcaaactctg
    18781 atacgcacct tggctcacgc cgccgttgcg cgtggtaatc agggttttca cattggcggg
    18841 cgcgggccaa tcggcggtca ggaagttttt gcctttcggg gcgagattta gggtttctgt
    18901 gatggttttc attggatatt ggaataggtg tgcggcggaa agggttgatt aactgccttc
    18961 ggggcaaatg gatgcttgtt ttttcgtcat tcccgcacag gcgggaattc ggagattcgg
    19021 gattgttttc aaacgttcgg gtttggattc ttgccggtgc gggaatgatg cccttatata
    19081 ctttctccga gctttatatg cttcaacagg ggacggcaca tcaagcaccg cactgcgtgt
    19141 tgcccgaacg aggctgccct ccgattagat tctatcgcta taaacagacg ggtttcaacc
    19201 gaaaaggaat ggggataaag tccatttccg acacctctcg ggcgatgccg tctgaaaacc
    19261 aatctccact ttcagacggc attgtttagg cgcggtcatt ttgcaaatta aggttaaggc
    19321 gtgtatgctt gccttccctt aaaaacaaac acgcaacgca ttattatgaa tatcctcagt
    19381 aaaatcagca gctttatcgg aaaaacattt tccctctgaa cctcgctctt tgccgccgcc
    19441 gcttttttcg cgcccgacac cttcaaatgg gcggggcctt atattccttg gctgttgggc
    19501 attattatgt tcggtatggg tttgacgctc aaaccttccg acttcgatat tttgttcaaa
    19561 catcccaaag ccgtcatcat cggcgtaatc gcacaattcg ccattatgcc ggcaaccgcc
    19621 tggctgccgt ccaaactgtt gaacctgcct gccgaaatcg cggtcggcgt gattttggtc
    19681 ggctgctgcc cgggcggtac ggcttccaat gtgatgacct atctggcgcg cggcaatgtg
    19741 gctttgtcgg ttgccgttac gtctgtttcc accctgattt ccccattgct gactcccgcc
    19801 atcttcctga tgcttgccgg tgaaatgctg gaaatccaag cggccggtat gttgatgtcc
    19861 atcgtcaaaa tggttttgct ccccattgtt ttgggtttga ttgtccataa ggttttgggc
    19921 agtaaaaccg aaaagccgac cgatgcgctg ccgctggttt ccgttgccgc catcgtgctg
    19981 attatcggcg cggtagtcgg ggcaagcaaa ggcaagatta tggaaagcgg cctgctgatt
    20041 tttgcggttg tcgtactccg caacggcatc ggctacctgc tcggcttctt tgccgccaaa
    20101 tggaccggcc tgccttatga tgcacaaaaa acgctggcca tcgaagtcgg tatgcaaaac
    20161 tcgggcctgg ccgccgcgct tgccgccgca cactttgccg ccgcgccggt cgttgccgtt
    20221 ccgggcgcat tgttcagcgt gtggcacaat atctccggct cgctgctggc aacttattgg
    20281 gcggccaaag ccggtaaaca taaaaaaccc taaacgcctt tcatcataat gccgtctgaa
    20341 cggggcaacc gaaaccggcg gtttctgccc ggccccttca gacggccttt gtttgaaatc
    20401 ggcggggacg attgcccggc tgctccggtt cggctgcctg cgttcgcccc gcctttcaag
    20461 ccgcctcact cccttacata aaccacttcg acatcgtagt cgtcttcgtt ccaatcgtca
    20521 tcgtcgtccg cgccgaattt gtcctgccat tcctcttcat tgctcaaaga cgaatccaaa
    20581 ccggcttcaa gacggaggac ggacaacaaa tgatatatgt cgtccggaat cggtgcctca
    20641 aacgaaacgg tttcgccgct ttccggatgg gtaaaactca agcggtaggc gtgcaacgcc
    20701 tgacgcgcgc ccaaactttt aacggcttct ttcaccgtgt cgccgcacgg atggcgcggg
    20761 ttgccgtaaa ccgggtcgcc ggcaagcgga tggttggcct cgcgcatatg gacgcggatt
    20821 tggtgcgtcc tgcccgttcc gagcgagcat tcgatgtagc tgtgggcaag atagcgttcc
    20881 aacactttga cgtgggttac ggcaggcttg ccgccgaatt tgacggctgc cattttcagg
    20941 cggttgtgcg gatcgcgtcc gatttgggtt tcgattttac cgtcgaaggg gacgatgccg
    21001 ttggcgacgg cgcggtagat gcgtttgacc gtgcgttctt gaagctgcct cacgagggaa
    21061 ttttgcgccg gcagggtttt ggcaaccacc atcagcccgc tggtttcttt gtccaaacgg
    21121 tgtacgatgc ccgcgcgcgg aatttgggac agctcggggc aatgcgccaa caggccgttg
    21181 agcagcgtcc ccgtccagtt gcccgccgcc ggatgcacca ccagtccggc cggtttgttg
    21241 acgacgatga cggtatcgtc ttcgtaaaca atatccaaat ccataggctc cggaacaaac
    21301 gccagatttt cctcactcgg acggacggtt acacaaattt gctcgccgcc tatcattttg
    21361 tctttgggtt gcgaaggttt atcgtttaca ataaccgcgc cttctttaat ccatgatgtc
    21421 aggcggctgc gcgagtagtc gggcagaagt ttcgccaaca ccgcatccaa ccgcccgccg
    21481 gcaagctcgg gcggaacggt cagaccgata cggttttcag ttccggaagc tgacgcaaag
    21541 tctgaatcgt cgctataatc ggattcatta tcaaaggaag tattctgcat gaaaaaaatt
    21601 cttttaacgg tttcattagg tttggcactg agtgcctgtg ccactcaagg tacggccgac
    21661 aaagatgccc agattaccca agattggagt gtggagaagc tctatgccga agcccaggac
    21721 gaattgaaca gcagcaatta tacgcgggct gtcaagttat acgaaatctt ggaatcgcgc
    21781 ttccccacca gccgccatgc ccggcaatcc caactggata ccgcatacgc ctattataaa
    21841 gacgatgaaa aagacaaggc tctggcggca atcgaacgct tccgccgcct ccatccgcag
    21901 catccgaaca tggattacgc gctgtacctg cgcggcttgg tgctgtttaa cgaagatcag
    21961 tccttcctga acaaactggc ctcgcaagac tggtccgacc gcgacccgaa agccaaccgc
    22021 gaagcgtacc aggcgtttgc ggaactcgtc caacgcttcc cgaacagcaa atacgccgcc
    22081 gatgcaaccg cacgcatggt caaactggtc gatgctttgg gcggcaatga aatgtcggtg
    22141 gcgcgttact acatgaaacg cggcgcatat atcgccgccg ccaaccgcgc caaaaaaatt
    22201 atcggcagct atcaaaatac acgctatgtc gaagaatcac tggccatatt ggaacttgcc
    22261 tacaagaagt tggacaagcc ccagcttgcc gccgatacgc gccgcgtatt ggaaaccaac
    22321 ttcccgaaaa gcccgttttt gacgcacgct tggcagcccg acgatatgcc ctggtggcgt
    22381 tactggcatt aagcccatcc tcgaaatgcc gtctgaagcc ctttcagacg gcattttccg
    22441 ttccggcacc agccttcccg ttcactcaaa cggttttttc tgccaataga atttatacac
    22501 tctcaaacgt tgagccttta cgcctccgcg tcccaagccg aattgcagcg cgcccgacaa
    22561 atccgtccgc aacaacttga tgccgtgtgc gcggacacgg ttctgcaccg cttcggtcgg
    22621 atgcttgtag gcgttggcat aaccgcttga agcaacggca tattcgggcg aaacggcatt
    22681 gaggaacacg cccgacgagg acgtattgct gccgtgatgc cccaacacca acacctggct
    22741 gtacaggttg cctccatact tgccgaccag gctttcctcg cccttcgtat ccaaatcgcc
    22801 cgttaccagc agtgccgcac cgcccgccac aacacgcaaa acacaacttt tcccattatc
    22861 atcgatattt ttgcgttcag acggcctcaa aaactcgaaa tccacgccgt cccattgcca
    22921 acgctgttcc gcacaatgcc gcgccccctc ataaaattcc ggctgtccgg cataaatccc
    22981 gccgttcggt atcttgccga ctgcctgaaa gccgccgtcg tggtcgctgt cgtgatgcga
    23041 cagaaccagc ttgtccaaac ggcggacacc cgccgcattc aaactcggca cgatacccgt
    23101 ttgtgccgcc gctaccgttc ccgtatcgaa aagcagatgg cggtttgccg tccgcacaag
    23161 caccgacaag ccctgccccg catcccaaac cgtaaccgca gcttcgtttt caggtactgc
    23221 ttcaggacgg taggatacaa accctgccaa cagcaacacc gcccacggac gcaaacccaa
    23281 gccgcgcggc agcagcaaca gcaaagcggc acacaccgcc aacaccagca gcggcaaagg
    23341 tgcggcggca acggcaaact caggcgacac atcggcaagc cacaccaaaa accgcaaagt
    23401 gtattccgcc aaaaatgccc ccgcctgctg aagcggcgca aacggcacga ccgaacccag
    23461 caacgccagc ggggtcaaca cccaagaaaa ccacggtatc gacaccgcat tgaccaaagg
    23521 gctgaccaaa ggcagcgagg caaacagata accgagcaaa accagagaca acaccgaagc
    23581 cgcccactgc ccgcgcacgg cggtttgccg tttcccctcg tacaaacgcc ctgcacacgc
    23641 ccatatcagg gccgccacca aaccgaaaga cagccaagta ccgacaccca aaaccgccaa
    23701 agggtcgaac agcagcaccg ccgccaacgc ctgccaccac gtcgcccacg ccgacagtct
    23761 tcccctgcgc cacgcccacg cgaacgccgc caacatcaaa acactgcgct gcgtcggcac
    23821 ggaaaaaccg gcaagcagcg cgtaaaacag cgcgcccgca caccccgccg ccaaaaccca
    23881 cgcgcgcggc cgggcgggaa ggcgcgggga acaggcaagc agccgcttcg ccagccacgc
    23941 aaacagcacc gccaccatcg taacgtgcaa acccgagata ctgaccaaat gcgtcagccc
    24001 caacggccgg aacgcctgcc acaatccggg gcgcaatgcc gactgttcgc ccacgctcaa
    24061 cgcgcgcatc agcccgatgc cgtctgaaag cccgccgtcc gcatccgcct gccgccaatt
    24121 gcggctgatg cggctgcgcc aaaccgcaat cccccaaccg cttccgccat gcagcaaaac
    24181 cctgtccgca ccgaccgtcc ccacgccgcc taccccgttg gataatgccc acgcctcacg
    24241 gttcaaaccg cgcaggttca actccccgac gacggggtgc acgcgtgccg ttatccgcca
    24301 tctgctcccg accgcccatt cgcgccgttt gtagtccgac agcagcaaat caaacgtccg
    24361 accgccgctg tccacagcct ttgccgcaaa ctgcacgcgc cgcccgtccg acctcggcat
    24421 atccgtcact tccaccgtca aaggcacgcc tgaaaccgcc tccgcccgcc attgcgaaga
    24481 cagtgccgct tccgttctga atacgccgta agccgcgccc gccaacacgc acagcatcag
    24541 accggcaaac gcaaaacgcc ttgcaagcac agccaaaacc gcaaaagccg ccaaccaaaa
    24601 cggccaatgc ggtacaaccg acagtgcgaa cgaaactgcc acgccgacaa cccaacacgg
    24661 caaaaaccaa cgacggaaca cgtctcccca ctcccgatat aaaaaacaaa cgatgataaa
    24721 gcaaagccat aaaaccgtca taacaagatt tgaaaatgat aagaatgcaa acgaaaagcc
    24781 tcagtattat ccgagacaac ggcaaacttt ccatcgtcat tcccgcaaaa gcgggaatcc
    24841 aaagacaaaa agccgcagaa atttatcgga aagaaccgaa ccacaaaaac cggcttccca
    24901 ccttcgcttg aataacagaa caaagaaagc ggaaataaaa acatcaggat tcttttttaa
    24961 attggcaata aaacaccaaa atgaatgagt tacacaaaaa aatactcaac accacccaac
    25021 cggcgtaaaa tgcaaaacat tatcgctatt aaaacggtaa aaccttatga aagccaaacg
    25081 ttttaaaatt aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc
    25141 agaagcggca ttggtgagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa
    25201 caaaggcaaa ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac gaggtcaaaa
    25261 catcggcaac gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacaa
    25321 acgcatagca acggtagtcg atccccaata tgccgtcagc gtcaaacacg ccaaagcaga
    25381 agtccatact ttttattacg gccaatacaa cggtcataat gatgtagccg acaaagaaaa
    25441 tgaataccgc gttgtcgaac aaaataacta tgaaccccat aaagcttgga gtgcgagcaa
    25501 cttaggccgc ctcgaagatt ataatatggc gcgttttaat aagtttgtaa ccgaagttgc
    25561 gccgattgca ccaactgatg ccggcggcgg attggatacc tacaaagata aaaaccgttt
    25621 ttcctctttt gtcagaatcg gtgcgggcag gcaattagtt tatgaaaaag gggtttatca
    25681 tcaagaagga aatgaaaaag gctacgattt gcgcgatctt tcacaagcct atcgttatgc
    25741 cattgcaggt acgccttata aagatattaa tattgaccaa acaatgaata ccgaaggttt
    25801 gatcggcttt ggtaatcata ataaacagta ttcggcagaa gaacttaaac aagcactttc
    25861 gcaagatgcg ttaacaaatt acggcgtgtt gggcgatagc ggctctccgt tatttgcttt
    25921 tgacaaacaa aaaaatcaat gggtcttttt gggaacttac gattattggg caggttacgg
    25981 aaaaaaatca tggcaagaat ggaatatcta taaaaaagaa tttgcagata aaatcaaaca
    26041 acacgataac gccggcaccg tcaaaggtaa tggagaacat cattggaaaa ccacgggtac
    26101 aaacagccat atcggttcga cagcggtaag gcttgccaac aatgaaggag atgcgaacaa
    26161 cggacaaaat gttacctttg aagacaacgg cactttggta ttggatcaaa acatcaacca
    26221 aggcgcgggc ggcctgtttt tcaaaggcga ttacacagtc aaaggcgcaa ataatgacat
    26281 cacttggtta ggtgcgggga ttgatgttgc cgacggcaaa aaagtcgttt ggcaagtcaa
    26341 aaatccgaat ggcgacagat tggcaaaaat cggcaaaggc actttggaaa taaacggcac
    26401 aggcgttaac caagggcaat taaaggtcgg cgacggtacg gttattctga atcaaaaagc
    26461 cgatgccgac aaaaaagttc aggctttctc ccaagtcggc attgtcagcg gacgcggtac
    26521 attggtatta aatagttcaa atcagattaa tcccgataac ctatatttcg gtttccgtgg
    26581 cggtcgtttg gatgccaatg gcaatgactt gacttttgaa cacatccgca acgtggatga
    26641 aggcgcgcgc attgtcaacc acaacacaga ccacgcctcc acaatcacac taacgggtaa
    26701 atctttaatt accaatccga acagcctttc ggtacatagc atacaaaatg actatgatga
    26761 agatgattat agttattatt accgaccaag gcgacccatc ccacaaggca aagatcttta
    26821 ttacaaaaac tatcgttatt acgccctaaa atccggcagc agcgtgaacg caccgatgcc
    26881 cgagaacggt gtagcagaaa ataacgactg ggtctttatg ggatatacgc aagaagaagc
    26941 caagaaaaac gcgatgaacc acaaaaacaa tcagcgtatt agcggtttta gcggtttctt
    27001 tggcgaagaa aacggaaaag gacataacgg cgcattaaac cttaatttca atggcaaaag
    27061 cgcgcaaaac cgtttcttgt taacaggcgg cgccaattta aacggaaaaa taagcgtaac
    27121 tcaaggcaat gtcttgttat caggtcgtcc aacaccacac gcaagagatt ttgtgaacaa
    27181 atcttcagcc cgaaaagacg cacatttctc caaaaacaat gaagtcgtat ttgaagacga
    27241 ctggataaac cgcacattca aagccgcaga aattgcggtt aaccaatccg catcattctc
    27301 ttccggaaga aatgtatccg acatcaccgc caacatcacc gcaacagaca acgccaaagt
    27361 aaatttgggt tacaaaaacg gcgatgaggt ttgcgtgcgc tcggactata ccggttacgt
    27421 tacctgcaac acaggcaact tatccgataa ggctttaaac agctttgatg cgacacggat
    27481 taacggcaat gtgaatttga atcagaatgc ggcattggtc ttgggcaagg ctgcattatg
    27541 ggggaaaatt caagggcaag gaaacagccg tgtcagccta aaccaacata gcaaatggca
    27601 tttgaccggc gacagccaag tacacaatct gtcattggcg gatagccata ttcatttgaa
    27661 caacgcttcc gatgcgcaaa gtgcaaataa ataccacacg atcaaaatca atcatttatc
    27721 cggtaacggg cattttcatt atctgacgga cttggcgaaa aatcttgggg ataaagtgct
    27781 tgtgaaggaa tccgcatccg gccattatca gctccatgtt caaaataaaa caggcgaacc
    27841 taatcaggaa gggctggatc tctttgatgc atcatccgta caagaccgct cccgcctttt
    27901 tgtttccttg gcaaatcatt acgtcgattt aggcgcattg cgttatacaa tcaaaacaga
    27961 aaacggtatt acccggttgt acaatcctta tgccgggaac cgccgcccag tcaagccggc
    28021 cccgtctcct gccgcaaaca cggcttctca agcacaaaag gcaacacaaa cggacggtgc
    28081 acaaattgcc aaacctcaag atattgtcgt cgcaccgccc agcccgcagg caaatcaagc
    28141 cgaagaagca aaacgccaac aagcaaaagc ggagcaagtg aagcgtcagc aagcagaagc
    28201 agggcgtaaa tcggcagagt tgtcggcaaa acaaagggcc ggggaagagg aacgccgtca
    28261 aacagcccaa tcccagccgc aacgccgcaa acgccgtgcc gcaccgcagg attatatggc
    28321 agtttcccaa gaccgtccga aacgccgcgg acaccgttcg gtacagcaaa acaatgtgga
    28381 aattgcccaa gcccaagcag aacttgcgcg ccgacagcag gaagaacgca aagcggcaga
    28441 gttgttggca aaacaaaggg cagaagccga acgtgaggct caagcattgg ctgcgcgccg
    28501 aaaagccgaa gccgaagagg caaaacacca agctgccgaa cttgcacatc gccaggaagc
    28561 aaaacgcaaa gcggcagaat ccgcgaaacg aaaagctgag gaagaagagc accgtcaaac
    28621 agcccaatcc cagccgcaac gccgcaaacg ccgtgccgca ccgcaggatt atatggcagt
    28681 ttcccaagac cgtccgaaac gtcgcggacg cagatctact ctgccggcac cgccctcgcc
    28741 atcatttgat tcatcagctt acgcagcacc cagggccttg cataatccgg actggtatga
    28801 aaatgattat gaagaaatcc ccttggatgc gttggaagat gaagatgtat ccgaatcggt
    28861 tgacacatca gacaaacagc ctcaagacaa tacggaactt catgaaaaag ttgaggcagt
    28921 gagtttgcaa ccaagagccg cgcagccgcg aacccaagcc gccgcgcaag ccgatgcagt
    28981 cagcaccaat actaactcgg ctttatctga cgcaatggca agcacgcaat ctatcttgtt
    29041 ggatacaggt gcttcattaa cacggcacat tgcacaaaaa tcacgcgctg atgccgaaaa
    29101 aaacagtgtt tggatgtcaa acaccggtta tggccgtgat tatgcttccg cacaatatcg
    29161 ccggtttagt tcgaaacgca cgcaaacaca aatcggcatt gaccgcagct tgtccgaaaa
    29221 tatgcagata ggcggagtat tgacttactc tgacagtcag catacttttg atcaggcggg
    29281 cggcaaaaat acttttgtgc aagccaacct ttatggtaag tattatttaa atgatgcttg
    29341 gtatgtggcc ggcgatattg gtgcgggcag cttgagaagc cggttacaaa cgcagcaaaa
    29401 agcaaacttt aaccgaacaa gcatccaaac cggccttact ttgggcaata cgctgaaaat
    29461 caatcaattc gagattgtcc ctagtgcggg tatccgttac agccgcctgt catctgcaga
    29521 ttacaagttg ggtgacgaca gtgttaaagt aagttctatg gcagtgaaaa cactaacggc
    29581 cggactggat tttgcttatc ggtttaaagt cggcaacctt accgtaaaac ccttgttatc
    29641 tgcagcttac tttgccaatt atggcaaagg cggcgtgaat gtgggcggta aatccttcgc
    29701 ctataaagca gataatcaac agcaatattc agcaggcgcc gcgttactgt accgtaatgt
    29761 tacattaaac gtaaatggca gtattacaaa aggcaaacaa ttggaaaaac aaaaatccgg
    29821 acaaattaaa atacagattc gtttctaaaa taccaaattc atagcaaaat aaaatgccgt
    29881 ctgaactcaa gcttcggacg gcatttttat cagactaaca aagctacagc tcaatgcctt
    29941 tgagtttcgc cacggtattg atgtctttgt cgccgcgacc cgacaggttg accaaaatca
    30001 cttggtcttt acccattttc ggcgcgtttt tcaccgccca agcaacggcg tggctggatt
    30061 cgagcgcggg gatgatgccc tcgaatcggc agagcaagtc aaaggcttcg agtgcttcgt
    30121 catctttggc aacggtgtat tcgacgcgct tgatgtcgtg cagatggctg tgttccgggc
    30181 cgatgccggg gtaatccaag cctgcggaaa cagagtgcgt ccccaagact tggccgtttt
    30241 cgtcctgcat cagatagctg cggaagccgt gcagtacgcc aatcggtgcg cccgaagtaa
    30301 tcggcgcggc gtggtcaggc gtatccacgc ccaaaccgcc tgcttctacg ccgaccaaac
    30361 gtacgttttc ttcttcgata tagggataga acaaaccgat ggcgttcgag ccgccgccga
    30421 cgcaggcaac ggcgacgtcg ggctgtctgc cgatagcttc ctgcatctgc gctttggctt
    30481 cgttgccgat gacgcattgg aaatcacgca ccatttcggg atacggcgcg gggccggcgg
    30541 cggtgccgat gatgtagaac gtatcgtcca ctcgggcgac ccattcgcgc atcgcttcgt
    30601 tcatcgcgtc tttcaaagta cggctgccac tgtcaacgcc gatgacattc gcaccaagca
    30661 gcttcatacg gaacacattc ggcatttggc gttgaatatc gtccgcaccc atgtacacgt
    30721 cgcaagtcat gccgaaacgt gcggcgacgg tggcagaagc cacgccgtgc tgacccgcgc
    30781 cggtttcggc gatgacgcgt tttttgccca tacggcgggc aagcaatgcc tgaccgatgg
    30841 tgttgttgac cttgtgcgcg ccggtgtggt tcaagtcttc gcgcttcaac cagatttgcg
    30901 cgccgcccaa atgctcggac aaccgcgcgg cgtggtaaac ggggctgggc ctgccgacat
    30961 aatgtttcaa atcgcggcgg aactccgccc aaaattcagg atcgtttttc gcttcgttgt
    31021 aggcctgttc cagctctttc aaagcgggaa tcagggtttc ggaaacataa agcccgccgt
    31081 gttcgccgaa aaagcccttt tcgtcgggcg cgtggtagtt cttcatcgga ttcttcctta
    31141 tgcttcaggt tttcagacgg catttccgtt ttttcaaaca tgccgtctga acgggaaatt
    31201 gtcggcaatt ataactgctt ttcacacaag cccgccattt cgaaaaacgc ccgatacgcc
    31261 gctgcctttt tcgccaaact caaaggatag gcgcgcgaag tcgaaggcag gcgcgtcagt
    31321 gtcaaatccc gtccggcaaa cggaaacggc acggtttcgc ccgttttcgg cattttgatg
    31381 ccgccgccct gaatatcgag caggatttcc gtcgccttgc cgcctgtcgt acaaatatgg
    31441 cggcagtctg gtattttcgc caacaccgcc gccaaatcga cggtttcaac tacctttaaa
    31501 aacttgtcgg acgcattgcc gtgttccctt gccgccttca acacggtcgg acaggacgca
    31561 atcccccgtt cgtgcaaaaa cgccttgatt ttctccgcgt caaacgcttt ttcagagtgc
    31621 gcagcctccc cgtgggacgg catccggaaa tgcgccgcat cgttaaaaaa caccagcccg
    31681 taaacgcgcc acatatcgtt ttggaaattc ggataatgaa actgcatcgc gcgtttgtct
    31741 tccttgggcg gaaacgtccc catcatcatg accgcagcct tcggcggcaa cacgggatcg
    31801 aagggatggg tttcgatttc aagcagattt tcagacacgt tcaatcctcc aacaggcaaa
    31861 gcggcagaca gccggtttgc accaaaccgc caaacacggc aagccttcaa acagcattat
    31921 cacgccctgc cgttttcggt tacaatgccg acatcaaacg gatactgtaa acacatttat
    31981 gttccaacac acaggacgac acataaagca ccgccctatg tgtcgtcctg atttggaagg
    32041 ggttacgccc cgcccaaaca gagtctgatc ctgccgccct aaagggcggg gtttcaaccg
    32101 aagaggaaac acgatgaaag aacacaaagc ccgcaagcgt ttcgggcaga attttttgca
    32161 ggacacgcgg attatcggcg atattgtcaa cgccgtgcgc ccgcaggcgg atgatgtcgt
    32221 gattgaaatc ggcccgggtt tggcggcaat taccgaacct ttggcgaaaa agctgaaccg
    32281 cctgcacgtt gtcgaaatcg accgcgacat cgtatgccgt ctgaaaacgc tgccgtttgc
    32341 ggataaactg gtgattcacg aaggcgacgt gttgcagttt gatttcaacg gcatctcagg
    32401 caaaaagaaa atcgtcggca acctgccgta taacatctcc acgccgcttt tgttcaaact
    32461 ggcggaggtg gcggacgatg tcgccgatat gcactttatg ttgcaaaaag aagtggtcga
    32521 gcgcatggtt gccgcgccga aaagcaacga ctacggtcgc ttgggtgtga tgctgcaata
    32581 ttttttcgat atggagctgc tgattgacgt gccgcccgaa tcgttcgatc ccgcgccgaa
    32641 aatcgattcc gcagtggtgc gcatgattcc ggtgaaacac cgtatcggca aggcggacga
    32701 tttcgagcat ttcgccaaac tcgtgaaact cgccttccgc caacgccgca aaaccatacg
    32761 caacaatctg aaagagcttg ccgacgacga tgatttgcag gcagtcggca tcagtccgca
    32821 agaccgtgcc gaacacatcg cgccggagaa atatgtggcg ttgagcaatt atctggcgga
    32881 caaggccgtc tgaaaaggaa cgcggcgatg gaaagcaacc tgcagaaaac cgagcgtttg
    32941 atccgcgaaa tcaaccgcct gcacgcacaa tattcgcagg attattttga aaccggcaaa
    33001 gtgcggaaaa tcaatttgag ccacacgctc aaaaacgttc cgaccgaaca tattttgtct
    33061 taccgcctta atttgcacga agcagttaac gattatttag cctttgccga tacgcgcggc
    33121 atagactttt tctaccgcgt gaaaaccgca gaaagcatac atgataaaat caaccgctat
    33181 ctggcacgcg gcacccaata tccgaccaac aacatcctga acgatatttt cggcgcgcgg
    33241 ctgatttggc cgtctgaaac agtcgccggg attttagaaa aacttgacgg ctggaaaaca
    33301 gaatacggct tgaaaaactg gtatttgcgc gatgccggcg gctatatcgg catacacatt
    33361 tatttcaaaa acagcagcaa tttctactat ccgtgggaat tgcaggtttg ggatgaaaaa
    33421 gatgccaaag ccaatattga aaaccatatg gcgtacaaac ggaatttcgt gcgttgaatt
    33481 gccggcaagc cgccttgaaa ccgtttataa ctgtttataa gagaataaac cataaaatac
    33541 acaaacgtat tcctgacttt tcctttaacc gcccgagtga acaaactatg aaatacacag
    33601 ccgcactcct gacttttctt ttaactgcct gcatgaaccc gaacgatgcg tttttccaaa
    33661 accggcgtta tcaaatgccg gaggcgcaat tgaacggttc aaacgccgtt ttccattacg
    33721 gttattccca aaatcccgat catgatttat tggtcgacca agtccgcgtg ccggacgcgt
    33781 acagggcttc atttgcgcgg gattggatag cgaaggagca aggttaccgc aaaaacgact
    33841 gcgtactgga aaaagcggaa agcgggggtg tgacgtacta cacctgcgat gcggaaaaag
    33901 gattcaacgg cacacgtttc gtcatctatg tcgtgaatat gaaaaacgac aaaggctacg
    33961 tcaaagttta ccggggcatg aataagccga atcaggaaga gttaaccaaa ttggttgccg
    34021 atttgaataa gttttaccct taatcagcca gacggattca gactgcccga aaagaatgga
    34081 cactatgatc aaattcaaaa acgtacacaa acatttcaaa gacctgcacg tcatcaacgg
    34141 cgtaaacttg gaaatcaaaa aaggcgaagt ggtcgtcgtc tgcgggcctt cgggcagcgg
    34201 caaatccaca ctcatccgca ccgtcaacca gcttgaaagc atcgaaagcg gcgagatttg
    34261 ggtggacggc gtcaacgttg ccgaccccaa aaccgatttg aacaaaatcc gcgaagaagt
    34321 cggctttgtg tttcaaagtt tcaacctcta cccgcacctg accgtgttgg acaacatcac
    34381 cctcgcaccg atgaaggtta aggggcaaaa tgcggaacag gcggagaaaa aggcaatgga
    34441 gcttttggaa cgcgtcggac tggcacacaa aaaagacgcc ttcccttccc aactttccgg
    34501 cggccagcag caacgcgtgg cgattgcgcg cgggctggcg atggagccgc gcgtgatgct
    34561 gtttgacgag ccgacttccg cactcgaccc cgaaatggtc ggcgaagtgt tgaaagtgat
    34621 gaaagacttg gcggaaagcg gcatgacgat gatgtgcgta acccacgaaa tggggtttgc
    34681 ccgcgaagtt gccgaccgcg tgattttcgt cgataaaggg caaatccttg aagacgaaac
    34741 gccggaagcg tttttcacca acccgaaaca cgaacgcgcc aagcagttcc tgcaacaagt
    34801 catgacccat tgatgctatg ccgtctgaaa accgaacgtt cagacggcat agcccgacac
    34861 cgcaagatga ttgagaaagc acaccatgta ttttgttgac cgcaccgccg ccgtcctcaa
    34921 gccgaccgcg cgctttttgg aatggctcaa aagcaccgac gaaaatatgc ccgacctgac
    34981 catagaacag cttcgcgccg actgttccgt cttcctcgtt ccgcagttcg acgaaccgga
    35041 agccgtcgtt tcctacttcg acgaacgcta ccggcagatt tttgaggcgg agctggcagg
    35101 ttgggacatc gacaaggaca aatggccgca agatatgggg ctgaaggcgt tttgggaatt
    35161 tttcgacatt gaaatccacg atatggtttt ggatatggaa gaagcggatt tgaacatcac
    35221 gcccgtgttc gacaatatga tgtaacgcca tgcgcgcctt tcagacggca ttgaggccgt
    35281 cccgcatcct gaacatcctg accgtatcac tccactcggc ttccattgcc gtctgcctga
    35341 cttggtttta cggccggatg atgtggttcg gtttggcggc tttggcggca agttacgcat
    35401 actcgctcag gataacgaat ctgaaacgcc gccatgccat aaccgccatt accatagacc
    35461 gcgacggacg ggcggaaatc gtatccggca aagacaaaac agcggcaaca cttgcgggca
    35521 gcagtatggt tacgccttat gccttattct tacaatggga cacaggcggc aaaaccgtcc
    35581 gccaatgcat catgcccgac atgacggaca aagaatccta ccggcggctg aaagtctggg
    35641 tgctttggcg gcagccgaaa aaaaccgccg aaaccgatac atcagactga tttcagacgg
    35701 catttgaaaa cgaaacactc atgaaaacat tacaagactg gctctcgcat ttggaaaccg
    35761 cccacagcgg cggtttgatc gacatgggtt tggagcgcgt aagcgaagta aaaaagcgca
    35821 tgaacctgac accgcaatgc cccgtcgtcg tcgtcgcggg aacaaacggc aaaggttcgg
    35881 tctgcgccta cctgacacag atttacaaac aggcaggctt caaaaccggc acgctgacca
    35941 gcccgcattt attgcattac aacgaacgca tcgccatcaa cgccgaaccc gtttcggacg
    36001 atacgattat tgcctctttc gagcgcatcg aagccgcgcg cggcgaaata tcgctgactt
    36061 attttgaatt caatgccttg gcggctgtcg acattttcat acgcgaacaa gtggatgtaa
    36121 tgatattgga agtcggcttg ggcggacgtt tggacgcggt caacgcgttt gacggcgatt
    36181 gcgcggtggt tactagtgtg gatttggacc atcaggcatt tttgggcgat acggtcgaac
    36241 aggtcggctt tgaaaaggca ggcgtgttcc gcagcggcaa acccgccatc tgcggtcaaa
    36301 accccgcgcc cgcatcattg gtcgcacacg ccgaagccat aggcgcgaaa ctgctgatgg
    36361 tacagcgcga tttcgagttt cacgcgatgg aaaacatcca atggaactac cgcttccggc
    36421 cgcagcattc agacggtccc gcacgcaacc gcaatgccct gcccttcccc gcattgcgcg
    36481 gcgcatacca gctttctaac gccgcctgcg cgttgaccgt attggaatgc ctcgacgaca
    36541 gactgccggt ggacattggt gcaatcaaac gcgggctgct gctggttgaa aatcccggac
    36601 gcttccaagt cctgcccggc cggccgctga ccgtattgga tgtcggacac aacccccacg
    36661 ccgcccgcgc cctgcgccgc aacctaatca accttgccta cgcgcaaaag cgcaccgccg
    36721 tgttcagtat gctgtccgac aaagacatag acggcgtgtc ggaaaccgtt aaagaccagt
    36781 ttgacgagtg gtatatcgcg cctttggacg tgccgcgcgg tatgacggcg gatgcactga
    36841 aagccaaatt ggaacaacac cacatcgaaa acatacagac attcgccgcc gtccgcgacg
    36901 cgtaccgggc ggcagcctca aaagcgggcg aagacgacag aatcgtcgta ttcggctcat
    36961 ttcataccgt cgccgacgta atgtcggtac tgtgaaaagg aaacttctat gtccgaaaac
    37021 aaacaaaacg aagtcctgac cggttacgaa cagctgaaac ggcgcaaccg ccgccgcctc
    37081 gtaacggcaa gctccctggt tgccgcctcc tgcatcctgc tggcagccgc actcagttcc
    37141 gatcctgccg acagcaatcc cgcaccgcag gccggcgaaa ccggcgcaac ggaaagccaa
    37201 acggcaaaca cggcacaaac ccctgccttg aaatccgccg ccgaaaacgg ggaaaccgcc
    37261 gccgacaaac cgcaggactt ggcaggcgaa gacaagcctt ctgccgccga cagcgaaatc
    37321 agcgagcctg aaaacgtagg cgcgccgctg gtgctgatta acgaccggct cgaagacagc
    37381 aacatcaaag gtttggaaga atccgagaaa ctgcaacagg cagaaaccgc caaaaccgaa
    37441 ccgaagcagg caaaacaacg cgctgccgaa aaagtgtcgg caactgccga cagtacggat
    37501 acggtagcgg ttgaaaaacc gaaacgcact gccgaaccca aaccgcaaaa agcggaacgc
    37561 actgccgaag ccaagcccaa agccaaagaa accaaaaccg ccgaaaaagt tgccgacaaa
    37621 ccgaaaactg ctgccgaaaa aaccaaaccg gatacggcaa aatccgacag cgcggtaaaa
    37681 gaagcgaaaa aagccgacaa ggctgaaggc aaaaagacag ccgaaaaaga ccgttcggac
    37741 ggcaaaaaac acgaaacggc gcaaaaaacc gacaaagcgg acaaaaccaa aaccgccgag
    37801 aaggaaaaat ccggcaaggc gggcaaaaaa gccgccattc aggcaggtta tgccgaaaaa
    37861 gaacgcgcct tgagcctcca gcgcaaaatg aaggcggcgg gtatcgattc gaccatcacc
    37921 gaaatcatga ccgacaacgg caaagtttac cgcgtcaaat caagcaacta taaaaacgca
    37981 agggatgccg aacgcgattt gaacaaactg cgcgtgcacg gcatcgccgg ccaggtaacg
    38041 aatgaatagc ctccccattg ccgacctcct cgcctccgcc gtcatcgccg cctgcatcgt
    38101 catttccacg atgcgcggcg tgattgcgga agcaggttcg atggtggcat gggtggtttc
    38161 cttctttttt gccaaactct ttgccgcacc cttcgccgac ctcgcctttg cctcgttcca
    38221 accccgcctg tttgcattgg ctctgtcatt catttccctg ttcgtcattg cctgtctgat
    38281 ccagaaaatg ctccgttcgc tgctgaccgg cgcagtttcg gcggtcggtc tgggctttgc
    38341 caaccgcatt ttgggcggtg tattcggtgc attgaaaggc gttttgattg ttaccctgct
    38401 gatcatgctt gcttcaaaaa ccgacctgcc cgataccgaa gaatggcaac agtcctatac
    38461 cgtaccgttt ttcgtatcgc tttccgaagc ggtgttaaac catacggaca acgcacccga
    38521 atccctcgac gacgactaat ccgcatacgt ggcgtttcag acagcatacc tgatttatgc
    38581 cgtccgaaac gccttttctt tccttgacac accccgacag acaaagcgca cctgcctgac
    38641 tttacacaaa tattgtcaac aattcgggca aaaagccgcc gtttgcttta taattccgtt
    38701 tcacccttaa acctcgaatc ggagaagacg atatgtgcgg cgtattaggt ttggtcagtc
    38761 atgaacccgt gaaccagctt ctgtacgacg gtttgcagat gttgcagcac agggggcagg
    38821 atgcggcggg cattgcaacg gcggaaggcg gtaccttcca tatgcacaaa ggcaaaggga
    38881 tggtgagcga agtgttccgc acgcgcaata tgcgcgattt gaccggcaac gccggcatcg
    38941 cccacgtccg ttatcccacg gcgggcaacg ccggcagcag cgcggaggcg cagcctttct
    39001 acgtcagctc gcctttcggc atcgttttgg cgcacaacgg caacctcacc aacactgccg
    39061 aactgtatga aaacgtgtgt aacaaacacc tgcgccacat caacaccggc tccgattccg
    39121 aagttttgct caacgtattc gcgcacgaat tgcgccgcga agtctctaaa aacgccgacc
    39181 cgcaccggct caatgccgac aatattttca acgccgttgc cgaagtccac cgcctggtgc
    39241 gcggtgcata cggcgtggtt gccatgattg cgggctacgg tatgctcgcc ttccgtgatc
    39301 cttacggcat ccgcccgctg gcattgggtt cgcaaaccga cagcgaaggc aggaaatcct
    39361 atgccgtcgc ctccgaatcc gtcgccttca acgcgcttgc ctacgatttg gaacgcgata
    39421 tccggccggg cgaagcggta tttgtcggct ttaacggcac aatcatcgcc cgacaatgca
    39481 gcgaccgcgc caaactcagc ccctgccttt ttgaatatgt ttattttgcc cgccccgact
    39541 ccgtgattga cggcgtgtcc gtttaccagt cgcgcttgga tatgggcgtg tccttggcgg
    39601 aaaaaatcaa acgcgagctg cccgtggacg gcatcgacgt cgtgatgccc attcccgata
    39661 ccagccgccc cagtgcaatg gagcttgccg tccacctcaa caaaccctac cgcgagggtt
    39721 tgattaaaaa ccgctatatc ggccgcacct ttatcatgcc cggtcagtcg acgcgcaaaa
    39781 aatccgtgcg ccaaaaactc agcccgatgg aaaccgaatt tgcaggcaaa agcgtgttgc
    39841 tggtggacga ctccatcgtg cgcgggacga ccagccgcga aatcgtcgaa atggtacgcg
    39901 cggcgggcgc gcgcaaagtc tatatcgcct ccgccgcgcc cgaagtgcgc tatcccaatg
    39961 tgtacggcat cgatatgccc acgcgcgaag agttgattgc caacgggcgc agcgcggcgg
    40021 aaatcgccgc cgaaatcggc gcggacggca tcgtgtttca agacttgggc gatttggaag
    40081 ccgtcgtcaa agcactcaac ccgaaaatcg aatccttcga ttcatcctgt ttcaacggca
    40141 tttatcagac cggcgacatc gatgatgcct acctcgaccg cctgtctgcc gagaaatccg
    40201 gctgcggcgg tctgaaaatc cacccgagca ggatggaaca cagcatcagc atcagcgatg
    40261 cgggcgacga agaataaggc ttcccaaacc gcgatgccgt ctgaaatccg aacgttgggt
    40321 tttcagacgg catttcccat atcggcatca catttcccgt ttcatccgca cacgatatga
    40381 gtaccgaaac caaaaactac attacgcccg ccggctggca ggcattgaaa gacgagcttt
    40441 atcaactggt caataaagaa cgccccgaaa tcgtccaaat cgtcaactgg gcggcaggca
    40501 acggcgaccg cagcgaaaac ggcgactatc tttacggcaa acgccgtatg cgcgaaatcg
    40561 accgccgcat ccgtttcctg accaaacgtt tggaggctgc cgtcgtcgtc gatcccgaat
    40621 tgcgcgaagc gaccgaccaa gtatttttcg gcgcgaccgt cggtctgttg cgcggcgacg
    40681 ggcgcgagca aaccgtcaaa atcgtcggcg tcgatgaaat cgataccgcg caaaacaaaa
    40741 tttcttggat ttcgccgttg gcgcgttgcc tgattaaagc gcgcgaaggc gacgaagtgg
    40801 ttttgaacac gccggaaggc cgtgaggaaa ttgaaatcct ttctgtcgaa tacatcaaaa
    40861 tcgactgatc gggaatgccg tctgaaacgg cacaagcgca ccgtttccca aataaaggaa
    40921 taccgaatga ggaaaatccg caccaaaatc tgcggcatca ccacaccgga agacgcactg
    40981 tatgccgccc acgccggcgc agacgcattg ggactggttt tttaccccca aagcccccgc
    41041 gctatcgaca tcattaaagc acaaaaaatc gccgccgcac tgccgccgtt tgtcagcgtt
    41101 gtcgcccttt tcatcaacga aagcgcgcaa aacatccgcc gcatccttgc cgaagtgccg
    41161 atacacatca tccaattcca cggcgacgaa gacgatgcat tctgccggca gttcgaccgc
    41221 ccctatatta aagccattcg tgttcagacg gcatcagaca tccgaaacgc cgccacgcgc
    41281 ttccccaacg ctcaggcact gctgttcgat gcctatcacc cttcggaata cggcggcacc
    41341 ggacaccgct tcgactggac gctgttggcg gaatattcgg gcaagccgtg ggtgcttgcc
    41401 ggcgggctga cccctgaaaa cgtcggcgaa gccgtccgca tcaccggagc ggaagcggtc
    41461 gacgtatccg gcggcgtgga agcgtctaaa ggcaaaaaag accccgccaa agtcgccgcc
    41521 tttatcgcaa ccgccaaccg cctatcccgt taaagcaaca aaaattgccg ccggaatgac
    41581 ttatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttgccgtac tatttgtact
    41641 gtctgcggct cgccgccttg tcctgatttt tgttaatcca ctataatctc aaaaatttat
    41701 gccattaaat caataatttc tgatgaattt tgaaaactta atcccgtcat tcccgcgcag
    41761 gcgggaaccc ggttcattga gcttcagcta tttagaataa attttgaaac tctaatcgcg
    41821 tcattcccac gaaagtggga atccggtttt ttgagtttca gtcatttccg ataaattgcc
    41881 ttagcattga atgtccggat tcccgcctgc gcgggaatga cggcggaaat cttgtttata
    41941 ttgaatcaaa aaaacctgca ccttaatcag ttggcggttt agtccgactt ttggggtgca
    42001 gatcaagctt ccagacggca tttcctttaa aacttcattt cgagcgcgag gctgaagttc
    42061 ctgcccggtg cggcatacct tccatagttg ctgtcgccgc cgtgccggtt tgccgtgctt
    42121 tccgcagtct ggcgcaagga ttcccaagta acgtagcggt agttgccgat attgtagata
    42181 gccgccctca aggtcagccg ttttttcaga ttcagataag cggaaacatc tgccgttttc
    42241 caagaagacg taacccttcc tgccgaatat cgtttttgat cgcctgccag ataagcaagc
    42301 tcgtcagggt ttttcccttt ggaataggtc agcataatgt ttgcgcccca tttcccctcg
    42361 ggctggtcgt atccgaaccc caaaacataa cgcgacggct gtaccgcatc caaagcatag
    42421 ctgcggaggg acaagtccgg ccggttggat accgatttcg gtttgatacg gttgtacgcc
    42481 aatgtggtgt acaggccttc gggcagtttg ccgtatacgc cgttccagtc gattttcccc
    42541 aagatgttga tgccttgaag cgacatattt tgggcattgt aataatcgcg tatatcaatc
    42601 tctgtcaatc gtcctgctga atccggcaat ttggtttttt gatcggcaac ggcaatcata
    42661 tcggtataac ggttgcggaa actgctgatt tccaaaaagc cgaaatcgcc cttccactgc
    42721 aaaccgattt cccggttggc tgccttttcc gatttcaggg cgggacgctg ccagcctttc
    42781 ggataatcgt gataaatgtc tatcccgaaa agttcttgga aggaaggcgt tctgaagccg
    42841 ctggaggcgc ggtaagacac ggaaaaatgc cggttcggtt tgaacacgat gccgctgttc
    42901 cacgaacggt cggcataccg cccgctgcgg acgagttctt ccgacgtggt gaagtttttc
    42961 cggtcgtacc tgccgcccaa gctgaaatca aaatatttgc cgattgaaaa acggtcgttc
    43021 aaagaaatgt ggatattgct gccgttgatt tttcttggca cgcatttgcg ggaacgcagg
    43081 gtttcgatat agccgcagac cgacccttcg acgacttcgg gcttacccaa acggtactta
    43141 tcttgattct tttcatcgaa tcccgtggat tccgaaatcc ttgccgcatt gtgggaaagc
    43201 tgttcggggc gggagatcgc tttggaagca tcgtaaccga agcccaaagt cagatggtgt
    43261 ttcgtccatt tgtttttcag cgacttctca aacgaggcat tcaaaacatt gtgctgttcg
    43321 cggtagtgga aacggtcgct gctgtcgtag gaatacggtt tgtccgccga cgcgcggcag
    43381 gatttgtcca cggcaggata cacggcgcaa ttcagcttca gcgtgttgtt gtcggttgcc
    43441 acgccctgtt tgtcaaacga caacaccgcc ttgtccgccc agttgtccga gtatttctca
    43501 ttttcataac ggtacagcaa acccatacgg cggcggcggt gatgttcgtc aataaatttg
    43561 gtgcgggaat atttcaaacc tatgcccttg accaaatttt tatcgccctt ccactcttct
    43621 atattcggca caaaatacaa gccgtcgcgg aaatcgtcgc cgtcgtacac cccgctcttg
    43681 tctctaaact tttccgcctc gtccgtaccg taatactgtt tttccgtcat atcgcggatg
    43741 tcgtaacgct gtttggtatc ctcaaacacg ccgccgacat aatgcctgcc gccgaagcgg
    43801 tagcccagct tggcaagcca agagccgctg cggtaatcca tcggatcggg caatatcctg
    43861 ccgccgcccg tgtaggcttg ggcggacagg ttttcgtggc gcgcctgcgc ctcccgcacc
    43921 tgcgcctctt cttcagcact taaaggctga ttttgttcaa tacgttcttt tacccagcgg
    43981 ttgagctggc ggttcaaata tatcccgtag cccgccaatt ttgccacggg cttgagtgta
    44041 ttctcgccct ctactaagaa aaatggctct gttgtcttgc gtttaatatc gtatgtctgg
    44101 cggaacgcgt ccaaacggtc tatgccgtat tccaccccgt ccgcaatatc gccgtgcggg
    44161 cgcgtttccc gcccttggcg ttcggttcgg attagcagcc cttcccaacc gtctttgctg
    44221 aaccccgcgc cgagcgactt cataaattgg cggtttttac tgccgtaagc ggtttttgcc
    44281 tgtatccccc ggcttttgcc gtctgaaatc aggtctgccg cctctttggt gcggaaggcg
    44341 accgcgccgc cgagtgcgcc gctgccgtga tcggacgaac cggcgccttt gtcgatttcc
    44401 accgtgctga tgttttcata ttcgatttcg ttgattgcgc cgctgccgcc gcgtccgccg
    44461 tatccgctca acgatccctg cacggtaaac gcctgtattt gggcaacgcc gtcaaccgaa
    44521 accgccacac ggtttttatc tacgccgcgt atcgagtagc cgccgctcgc gccgttgccc
    44581 tgttcgacaa ccgccacgcc cggatcgtag cgcgtcaggt cgcggatacc gagtacctgt
    44641 tctttgttca acgtttccga cgttttgacg attttgccca aacctgtcgc ctctttcgat
    44701 cgccgtccca ctttggcggc acggacggta acctctttca atgattgact ttgcgccgca
    44761 tcaagtgccg ccgcccccgc ttgggcagca taagccggaa aagcggttgc aatggccaac
    44821 gccgtcaaag tcagcggaaa accgtgtttc ttattcattt ttccacctct ttattatctt
    44881 tcttcgcacc gaataccacg ccgaattggg gtttaacttc aggttctaac tgttcgccaa
    44941 catcagcttc attttcaact tcattttcaa tatcttcagt tataccaaga gatttcccat
    45001 ccttattgaa aatagtgccg cccaattccg ccgcctgcgg gccgtaaaag ccgcccgtta
    45061 caagaagatt gtcggctttg aaactttgag gattagtcga accatttccc gaaagattga
    45121 tgccgttatc ccgagtgcgt gctgttgcgt ggaaaccgtt gccctcaatc acgccgtttt
    45181 caatacggaa agcaggttct acaccgtttt gctccgtcag cgttccggaa atcgatttat
    45241 tgccgaaatc aacgtcaaat tctgcttttg ccgctttatc cgccttatta tcccattgaa
    45301 tgggttcgcc gatacgcgct tcccaagtgc cggtataatg cgccgttccg gtttttggaa
    45361 tgtcggcttc cgccgtgcgg atacctttca ggaaaaggtc gatgtccctg cctttaaggg
    45421 cttccggggc gggcgggatg ccgtctgaaa caccgttgcc ttcttccggc gattcttctt
    45481 cgggttcttc ctcttcagct tcttcttcga tttcttcagc ttcatcagct tcttcttcga
    45541 cgatttcgtc ttcgtcttcg ccgttatcat cttcggaaac ttcgtcttcg ctttcttcgg
    45601 cgatttcttc gtcttcgctt tcttcaccgt tattaatacc cgaatcctct tcgtcctgcg
    45661 ccttcggttt gacggcgggg cgttcggttt ttatccgtcc gagtttcaca taggtcaaaa
    45721 agtcgcaaca agcactgacg gtcattttcc tgccgtcggc aagatcgatg gttttctctt
    45781 tgctaaccaa aggaatttca cgcccttcga caagaagttt gtcaggatga ccaaaatcgg
    45841 gcatagtgga aacctcaaac ggacggggat tttcatcagt cgcctcgtca acggaaattt
    45901 ttagagaatc caagattttg gtgtgttttc caaacggcat agcaggattt gtgcctgatg
    45961 cgtttgtttt ttgtttgcct gcgaatacgc cgaatacgcc gttgtcgtta ctgataaacc
    46021 gtccggcaag ctcttctccg ttatcgccga aaaaaccgcc ctcaagccgc tgatcggcat
    46081 cggtatggaa aaacaaatat tctttatcag cgtgtctcgt cttcacctcg gtgttaactt
    46141 tggcactgcc ggtaaagcgg ttgccgttta aatcggcagt gatgttgtaa atggtcagcg
    46201 gtttttaggt tcctttggat catttctctt ttgcacatac tgatttttaa tcagctcgcc
    46261 ctttaggatt tttttgtcga aatcaaccgt atattcggca ggatgttttt ccctgtcgtc
    46321 ggcatcctta gccgcataag aagttgcacc aatatcgtta ccataaaatg ttatataacc
    46381 caaatcgtca ctggcaaccg ccttacctgt ccgatgacgt ttggcatcgg tcatatattg
    46441 ccagttgccg gaatattgca ccgttcccgc gctcggtaaa gattgggaag gacgttctcc
    46501 ggaataatat acaaaaccgt cataaccaaa acggtgggta aaccgcttac cacctgagtt
    46561 ctgttcgatt tcatcttttc cgtttttagt atatacataa ccggcatcca caaatttata
    46621 attatatttt ttattttcct tttctgacgt cctaatttct acatcggaat cacgtttatg
    46681 aatttcgctt ttaagttgtt g
//
LOCUS       DAWTHD010000002        43625 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWTHD010000002 DAWTHD010000000
VERSION     DAWTHD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43625
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.002"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            179..610
                     /locus_tag="WM704_000047"
     CDS             179..610
                     /locus_tag="WM704_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFA9339574.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
     gene            802..2841
                     /locus_tag="WM704_000048"
     CDS             802..2841
                     /locus_tag="WM704_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFA9339575.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            2856..3395
                     /locus_tag="WM704_000049"
     CDS             2856..3395
                     /locus_tag="WM704_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339576.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGVLLLLWLN
                     TDNPEAFHRLFMMLQDPAHFAKAISGLFRLFYCISFFGTFVKLVFNQDSIFSIFNKEL
                     SKNIQSNKTCSMDIVAVRD"
     gene            3499..4071
                     /locus_tag="WM704_000050"
     CDS             3499..4071
                     /locus_tag="WM704_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFA9339577.1"
                     /translation="MDRRNFLKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDR
                     TINCKRADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYA
                     MQEWARQSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELE
                     QMAKYYNVGGIGIYNSFVHLDTGRVRHWRG"
     gene            4335..4784
                     /locus_tag="WM704_000051"
     CDS             4335..4784
                     /locus_tag="WM704_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFA9339578.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            complement(4805..5128)
                     /locus_tag="WM704_000052"
     CDS             complement(4805..5128)
                     /locus_tag="WM704_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339579.1"
                     /translation="MTYGREGHSGKLPKGQPSNSCFKSNKNKKFGLLKNCIFLLMLTI
                     DMSIDKKTILLKAKSLRGLTLCFTIIQELLGKLNRRFDRGLIFQAMSWKTKNGEARKL
                     PTILK"
     gene            5969..6484
                     /locus_tag="WM704_000053"
     CDS             5969..6484
                     /locus_tag="WM704_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFA9339580.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKKPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            6494..7075
                     /locus_tag="WM704_000054"
     CDS             6494..7075
                     /locus_tag="WM704_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339581.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            7301..7708
                     /locus_tag="WM704_000055"
     CDS             7301..7708
                     /locus_tag="WM704_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339582.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARISSFEKSVDARFSSFENSVDARISSFENS
                     VDARFSAIDTKINAIYWFIGITTTVAVAAAKFL"
     gene            7947..8225
                     /locus_tag="WM704_000056"
     CDS             7947..8225
                     /locus_tag="WM704_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFA9339583.1"
                     /translation="MTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAREA
                     AQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            8227..8718
                     /locus_tag="WM704_000057"
     CDS             8227..8718
                     /locus_tag="WM704_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9339584.1"
                     /translation="MPLPDFRIRLLDSADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRIGKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            8814..11297
                     /locus_tag="WM704_000058"
     CDS             8814..11297
                     /locus_tag="WM704_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFA9339585.1"
                     /translation="MYNPVSKIDELLPDFAGLDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALGRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEDSVEVQLAKRFVDRQD"
     gene            11545..11802
                     /locus_tag="WM704_000059"
     CDS             11545..11802
                     /locus_tag="WM704_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339586.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            12355..12579
                     /locus_tag="WM704_000060"
     CDS             12355..12579
                     /locus_tag="WM704_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339587.1"
                     /translation="MRYSKNKDYQFFIRQLVSGGEWMFLPKNGRKHSALKHLPTDRKI
                     PIPGSPGQDPRGLLNFKTMVRHIERGGTFD"
     gene            12650..13771
                     /locus_tag="WM704_000061"
     CDS             12650..13771
                     /locus_tag="WM704_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9339588.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTSGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            13848..14189
                     /locus_tag="WM704_000062"
     CDS             13848..14189
                     /locus_tag="WM704_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339589.1"
                     /translation="MEQLDSAAPAACDTPSLFDLGRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            14425..15006
                     /locus_tag="WM704_000063"
     CDS             14425..15006
                     /locus_tag="WM704_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339590.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGKGVSAVADMR
                     YLMPAYRQYFNRPDKMVIFQGALQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGF
                     SLSDVLTDGCRHVLREVVTGWLNREILPELYNV"
     gene            15098..15613
                     /locus_tag="WM704_000064"
     CDS             15098..15613
                     /locus_tag="WM704_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339591.1"
                     /translation="MSIKVDNILLIEGTKESVAEAAKLILFRSPVHPEHWAFDFNRIL
                     PLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIEICFLTGLAPPFGIYRKLAE
                     IFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMADIRKFGIAEFDIWYEGLSD
                     RLESDGYLVED"
     gene            16184..16980
                     /locus_tag="WM704_000065"
                     /pseudo
     CDS             16184..16980
                     /locus_tag="WM704_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17069..17533
                     /locus_tag="WM704_000066"
     CDS             17069..17533
                     /locus_tag="WM704_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339592.1"
                     /translation="MVENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLYTAVRLLNENQGILHGISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESRLDACLK"
     gene            17937..18248
                     /locus_tag="WM704_000067"
     CDS             17937..18248
                     /locus_tag="WM704_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFA9339593.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            18250..18699
                     /locus_tag="WM704_000068"
     CDS             18250..18699
                     /locus_tag="WM704_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFA9339594.1"
                     /translation="MPDSPMQINGYTIYEHECFTRQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            18814..19884
                     /locus_tag="WM704_000069"
     CDS             18814..19884
                     /locus_tag="WM704_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339595.1"
                     /translation="MSRTNLKGGRKIALWRQTGYIGFYCCLILMMLFFPKDFNRLKII
                     AYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYSEADSG
                     GASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSEYRIDI
                     DGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALSKIGIP
                     VVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRNPLSAD
                     FQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQNFQAH
                     SGKREIPKKHGILQRMASWFKS"
     gene            19937..20551
                     /locus_tag="WM704_000070"
     CDS             19937..20551
                     /locus_tag="WM704_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339596.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQAYRSKVTDVQP
                     GKKISTSYGSALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            20606..20968
                     /locus_tag="WM704_000071"
     CDS             20606..20968
                     /locus_tag="WM704_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339597.1"
                     /translation="MECKLKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEATLNLFV
                     EGFWIIGLLQALAFTTIVDYCTESKHTNMLNNPACNDEDWDNDMLNNPACNDEDWDND
                     MLNNPAYSWSMFNIHHSD"
     gene            21172..21759
                     /locus_tag="WM704_000072"
     CDS             21172..21759
                     /locus_tag="WM704_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFA9339598.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVSNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNRIFRNMKPGSDTDVAT
                     NDDWLKDFWSEDSLIYDPAYSNLNCNIFHRSDDDI"
     gene            21806..22129
                     /locus_tag="WM704_000073"
     CDS             21806..22129
                     /locus_tag="WM704_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339599.1"
                     /translation="MKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEVTLNLFVEGFW
                     IIGLLQAAVFTFVVDCSSSDLGSLSQWQIGNDWDRQMDPFYAHEIGNIWHGTHLDSSS
                     EEYWK"
     gene            complement(22186..22344)
                     /locus_tag="WM704_000074"
     CDS             complement(22186..22344)
                     /locus_tag="WM704_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339600.1"
                     /translation="MSIAQIVVQIEEVDEGNYQVRMSYSGGNDLVSEEGKSAFEQIIT
                     SIGKVLET"
     gene            complement(22348..22893)
                     /locus_tag="WM704_000075"
     CDS             complement(22348..22893)
                     /locus_tag="WM704_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFA9339601.1"
                     /translation="MWRGISSGRHVRGNYVLKASTAHNQAELERYVHNPQVRAFLDFI
                     AASEGVKHGYQTAIGNYRITDFSRHPGNSVRRSFTRTDGSRSTSDASGRYQFMGYTWK
                     GLAKQYGFTDFSPATQDKAAVALILAKKGAMEAILNGDYEQAVMKLGGIWASFPTAPN
                     EYRQHKRSWGFVHNFFRQRGF"
     gene            complement(22935..25937)
                     /locus_tag="WM704_000076"
     CDS             complement(22935..25937)
                     /locus_tag="WM704_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFA9339602.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMYQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALVT
                     LGNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGDSRAQMRNSSFGVSSQSQISQSESYSKAADR
                     AETSAQQWQASFRESVSQGNANRLAFATNYGMDRSASEGYGMGLDAAESRKLSYVIDQ
                     GKKIAQKFGVTNTSAVISAIAAGLGISGNTGSSTKAQNIKNALGAKGDLGWSGKSQNT
                     DQMVAEIGESIEAGKRQGISFDSNFTDKVSQSEAYKVALNSGNSLAQTAEANFSKARE
                     ASHMASESYQKAQSYREMAAQTYGNGITIAQDNTNALVQDNPELTMSELNAGQGGTYN
                     AAIRAGVNSQDRLKAAAENSGWKPNDLSPEDRPQNNTQAAYKHFSDKVSSGAGAAFGD
                     VQTQAHQAGIIPDGISAAINARGENLKQEYANKSDKVEGDINQGRNSHRQEVGRLATH
                     AKNAQDQTNGVTMRINTHALPWNSFGERYDNAMKDVREGKGYIGSGSDQAAAHLGNPA
                     LASEFDLPIPQRPGNSKSSGSAVFRPKE"
     gene            complement(25944..27455)
                     /locus_tag="WM704_000077"
     CDS             complement(25944..27455)
                     /locus_tag="WM704_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFA9339603.1"
                     /translation="MKFHIFRFPKPAKVPTVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGAWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            complement(27627..28418)
                     /locus_tag="WM704_000078"
     CDS             complement(27627..28418)
                     /locus_tag="WM704_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFA9339604.1"
                     /translation="MKRVRMAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWY
                     PDAKKPKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEV
                     VMNKSAAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGEN
                     GIMFVFKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLN
                     ITTVPAMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            complement(28415..28840)
                     /locus_tag="WM704_000079"
     CDS             complement(28415..28840)
                     /locus_tag="WM704_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339605.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            complement(28827..30410)
                     /gene="traN"
                     /locus_tag="WM704_000080"
     CDS             complement(28827..30410)
                     /gene="traN"
                     /locus_tag="WM704_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFA9339606.1"
                     /translation="MRAITSLLVMICHFMLITSASAAALRESAACTRTSSVCVDGPST
                     KNINGVDVTKDCWEYKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLR
                     YTHKYSCDVDLRTLHQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEP
                     GSTKTINGVPVTRDCWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTC
                     QAREKQFECTEKGETTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQ
                     CSIKLDGALDSVFGGDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFV
                     SSAAQAMGTALSAAGGITGTSQIGALGFSAAGQQGMGVIVGFNPAVFAAAIAVIAIQQ
                     WLKCPQSEILVAMKRKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGK
                     EQLKRGWGTPENPKCEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKI
                     REASVNGKNLEVKNYYEYQ"
     gene            complement(30407..31651)
                     /locus_tag="WM704_000081"
     CDS             complement(30407..31651)
                     /locus_tag="WM704_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339607.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTRLGNAERGKSWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            complement(31648..32400)
                     /gene="trbC"
                     /locus_tag="WM704_000082"
     CDS             complement(31648..32400)
                     /gene="trbC"
                     /locus_tag="WM704_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFA9339608.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            complement(32415..33533)
                     /locus_tag="WM704_000083"
     CDS             complement(32415..33533)
                     /locus_tag="WM704_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFA9339609.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTAPIPNKLTCQGKM
                     LNPINDICWSCMFPIKIGGKINLNMDNQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADTAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCMEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAAAGFPFNKLYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMA
                     ARIQTKMHRDLVAWRTSGRDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            complement(33530..34186)
                     /locus_tag="WM704_000084"
     CDS             complement(33530..34186)
                     /locus_tag="WM704_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFA9339610.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPSVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            complement(34195..34740)
                     /locus_tag="WM704_000085"
     CDS             complement(34195..34740)
                     /locus_tag="WM704_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFA9339611.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            complement(34740..35288)
                     /locus_tag="WM704_000086"
     CDS             complement(34740..35288)
                     /locus_tag="WM704_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339612.1"
                     /translation="MTEAKEKKENTANTDPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            complement(35316..37892)
                     /gene="traC"
                     /locus_tag="WM704_000087"
     CDS             complement(35316..37892)
                     /gene="traC"
                     /locus_tag="WM704_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFA9339613.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            complement(37902..38483)
                     /locus_tag="WM704_000088"
     CDS             complement(37902..38483)
                     /locus_tag="WM704_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFA9339614.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            complement(38480..39199)
                     /locus_tag="WM704_000089"
     CDS             complement(38480..39199)
                     /locus_tag="WM704_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFA9339615.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            complement(39209..40522)
                     /locus_tag="WM704_000090"
     CDS             complement(39209..40522)
                     /locus_tag="WM704_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFA9339616.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRPIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            complement(40524..41258)
                     /locus_tag="WM704_000091"
     CDS             complement(40524..41258)
                     /locus_tag="WM704_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFA9339617.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMASMMRGTTGD
                     MGINHSHEYQTVPLWKNTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            complement(41260..41910)
                     /locus_tag="WM704_000092"
     CDS             complement(41260..41910)
                     /locus_tag="WM704_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFA9339618.1"
                     /translation="MLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTIVQTTL
                     TPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSPDYKDA
                     LERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQKDRELL
                     ISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGNRGN"
     gene            complement(41922..42203)
                     /gene="traL"
                     /locus_tag="WM704_000093"
     CDS             complement(41922..42203)
                     /gene="traL"
                     /locus_tag="WM704_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFA9339619.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            complement(42225..42521)
                     /locus_tag="WM704_000094"
     CDS             complement(42225..42521)
                     /locus_tag="WM704_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HFA9339620.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            complement(42577..43098)
                     /locus_tag="WM704_000095"
     CDS             complement(42577..43098)
                     /locus_tag="WM704_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFA9339621.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            complement(43068..43529)
                     /locus_tag="WM704_000096"
     CDS             complement(43068..43529)
                     /locus_tag="WM704_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9339622.1"
                     /translation="MRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWILA
                     NSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
BASE COUNT        11455 a        10028 c         9706 g        12436 t
ORIGIN      
        1 gtgccgatga ctgtccgtgg gcattttagc tagatatagc ataaggttta atttgtaata
       61 gttgctagtt gacaagagga atttatttag ttatgataat agaaccctta atctcatatt
      121 gggttatttt accctgtctt ttctgtcata tttttaaatc ctttttttgg agaaaaatat
      181 gtcagttcaa ctttttgttc gtggcaatct cggtgctgaa tttaatctgc gtcctgtagc
      241 aagccctaaa aacgatgaag cctcaatcgt actgaatttt tcagttgcct cacctaattt
      301 caaacgcaat gccgaaggta aatcagaagt ggtttcaacg gagtggattt cttgcgaata
      361 ctggaatcgt gatgctgccc atctccataa aatattacag gtcggtatgc cggttttcgt
      421 taccggtgaa gaacgttacg agtcttatac caatcgtgac ggtattgaag ttcgcgttcg
      481 taaattgcgt gttcaagatg tattcattgt tccaactgag cgtgtagaag ctatcaagtt
      541 gcgtcctaaa cgtgagcagt ctcaggacaa taatcacgtt gaagacgctg atgatgacat
      601 cccattgtaa atatatggcc acgaagaata cttccttttt tcgtgaaatg aactgttggc
      661 ttgctgcatt atgggctgac tttaaggata tgttcattgt tacccttatt tatggtggct
      721 taattgcttt ggcaatttgg gcttttttga ctatcgcatc ttggcttgtt ttgcagctga
      781 agcaaagctt gggattttaa aatgcgtttg tttttatgtg aaaaaccatc tcaagctaag
      841 gacatcggta aagtgcttgg tgttcttggc gggaggcatg atggttattt tcagaatgga
      901 gacactattg ttacctgggc atttggtcat atcctgacac aggccgcgcc agatgcttat
      961 ggcgaacaat atgctgactt tggaaatatc tccgcattgc ccattctccc tgaagtttgg
     1021 cagatgcagg taccgccgaa ggtatcgaag cagtttaaag tcattaaaag cttgttggcc
     1081 aaagcagatg aagtagtgat tgctactgat gcagaccgtg aaggggaagt tattgcccgt
     1141 gaaatattgg actactgtgg ctaccgtaaa aagatttccc gattttggac gagtggccta
     1201 gatacccaat cggtaaaaaa agccttatct tcaataatgc cgggtaagga taaagaaaac
     1261 ctatatcagg ccggtttggc gcgcggccgc gccgactggc tggtaggtat gaacctgagt
     1321 cgtgcctaca cagctgctta ttctgccgga tatggccgag aacatatttt gtcgatcgga
     1381 cggattcaaa caccgacttt aaagcttgta gtccaaaggg ataaagagat cgatactttc
     1441 gtgccttaca actactacac cctttcagta gattttgaga cagcagacaa gtcagtgttt
     1501 actacactgt ggcaggttcc tgaaaactgg cgtaatactg acggatactg taccaacctt
     1561 gaggctgtaa aggcacttgt caaacagctt gagaaatcta caggcaaggt tattacctca
     1621 aataaagagc gtaaaagcac cccgcccccg ttaccgtaca gcctttcggc tttgcaaaaa
     1681 gaagccggta aaaaacttgg cctatcggct tccaaagtgc tcaaaattgc acaaaccctc
     1741 tacgaaaaat acaagatcac gtcatatccc cgcacccctt gcagatactt gccaaaatcg
     1801 cagatgagtg aagtgaaaga tgtatttgcc gcgatacaga ctatcgataa atccatgctt
     1861 tcagtgattg aaaaagctga tcctgaacgt caatcaaggg tatggaacga taaccaggta
     1921 aacaaacatt cccatcatgc gattattccg accaaggcca gtaattttga tttgtcagtc
     1981 ctggataaga atgaattaac ggtgtaccga atgatacgag accgttatat cgcacagttt
     2041 tatccagatt ttgaatatga ctctactgta gttgaggttg aggcatgttc acatttgttt
     2101 aaagcctcca gccaatcacc ggttatttcc ggttggaagg ttcttttggg caaggacgtg
     2161 tttgaaggcg atcaaatcga tgaaggtcct gctctaccac atctgaaagt caacgatgag
     2221 gtcgacacgc tttccttcaa tcccgaaact aaaaagacca cgccgccacc gcgctttaca
     2281 gaggcttctc tactcgatga gatgcagact ttgaaggact ttttgaagaa tgtagaagac
     2341 gaacagatca gaaaaattct caaaagtacg gaaggcttgg gaacggaagc cactcgcgct
     2401 accattatcg atcgtttgtt tgaaatgggt tatatggaaa agaaacggag caaaatctat
     2461 gcgacagaaa aaggccgtaa tctgattgcc cgtattccca ctatgattgc cgatccgata
     2521 acgacagcca aatgggagct ggctttggct gggatagaag ccggcaaatt aactttagct
     2581 gaatttatgg cttatcagca gaaagtgatc acagagctgg ttgggcaggc gaaaaaggat
     2641 gctgtaggaa aagctcgtcc gccaaaacag acggattcag ctggtacaaa aaaacaggcg
     2701 gtcgctcgaa atgaagacga tgtctgtccg acctgtaagg aaggccgtct gagacagaga
     2761 ggattcaagg aaaaccctga taagcggtat tgggggtgta gtaggtttcc cgaatgtaaa
     2821 cacttcgaat gggtcaaata aaaaaggact ttgaaatgaa gaagctggta aaaaaattat
     2881 ccgattcaat attcggaaat acttgtatag agtatgtgct agatgaaaac cataattaca
     2941 aggagcaata caacgctaat aaagatttat tcatttacat caatggcatt caagtaggga
     3001 gtatttcacc tggcgaactg caagaattgc aatatagagt gttttattct agtagcctct
     3061 ggtggagtat ctttaaatct atactaggat ttttattgaa acccgcaata attgcgctta
     3121 ctgccctgcc gctgggggtc ttgcttttac tgtggttgaa caccgacaat cctgaagcgt
     3181 ttcatcgatt attcatgatg ttacaggatc cggcacattt tgcaaaagca atatccggac
     3241 tttttcgtct gttttattgt atatcattct ttggcacttt tgttaaattg gtttttaacc
     3301 aagattcaat tttttcaatt tttaataaag aactttctaa gaatatacag agtaacaaaa
     3361 cgtgctcgat ggatattgtg gctgtcagag attgaggaag ctcaaagatg ttgccctcta
     3421 ccagcatact gggaagtttg catatcaata ctgagtgttg cttaaaattt gattggagtc
     3481 attggtttgg aatttcagat ggatagaaga aattttttga aaaagtttgt cggcgttggc
     3541 gccctatata cgaccggtgc tgccggtttg ttattgccta aagatgtagt agcggcaacc
     3601 agtatggctg atttttggtc acgagaccgt acaatcaatt gcaaacgggc agatactggc
     3661 gagaaacatg aaatccgttt ttttcagcaa caaaatggat atgatcttga tgcttaccgc
     3721 aatgcctgtt ggctgatgcg tgatgcgaaa gatggaaatg ccatggttca aattgacgta
     3781 gggcttttaa acctgatgta tgcaatgcag gagtgggcac ggcaatcagg acggtcaaac
     3841 cctgtgatta cgatcaatag tgcatataga acgccaagac gaaatgcgac tattgagggt
     3901 gctgctagaa actcacttca tatgcgtggt aaagcagttg actttactat gaggggggtg
     3961 ggcattggcg agcttgagca aatggctaaa tactacaatg tgggcggtat tggtatctat
     4021 aatagttttg tccatttgga taccggcagg gttcggcatt ggcgcggtta aagattttac
     4081 gaaaaaatat tgacatacga aattatcggg aatatattgt aatcgttttt gtagtttgtg
     4141 acttttctta aaagtctcgt catttagccg ttcatgaatg agattcgatt cgtcgaattt
     4201 acggtacttc tttttcagcc agcctttaag gcttctaagt cagttcggct accgtgaaag
     4261 ccgcaactta accctttggg gatatactgt cccctttggg attgatatat cccctgtttt
     4321 tttggagata tatcatgact caatctaatc aaaactactt taacctgact gcagatggta
     4381 tcggtttttt aaaccggcca cgtgaagttc aaggaaagag aaaatcttcc aactattatg
     4441 cttgtacgat ccaagcctcc cgaggtgata atggggagaa aacccgtttc gatcttcgta
     4501 tcgtcggagg tcaagcgaaa gagctctttg ctcgcctttt ggaagaattt ccgcaattgc
     4561 tgtctaaaaa ttaccgtgaa cgcccgactg tcgtggtcgg tttccgtgcc ggtgatattc
     4621 aacctttaaa atttgaaact aacaatcgtg agacgggcga agttttagaa actttaagta
     4681 tcgacggtcg gttgttgaaa ttcaaattta ttaaagtgga tggcgatatg tggtatcaat
     4741 catcctctca agatgaaacc caaaataatc ctgatgcttc ttagaaagct attgaatatt
     4801 ttgattattt aagaatggta ggaagctttc gggcttcccc gttcttagtt ttccagctca
     4861 ttgcttggaa aattaagcct ctgtcgaaac ggcggtttaa ttttccaagc aactcctgaa
     4921 ttatagtgaa gcaaagggta agtcccctca aagacttggc tttcagcaat attgtttttt
     4981 tatcgatgct catatctatt gtgagcatca gtaaaaaaat acagttcttt aacaatccga
     5041 attttttatt tttgttgctt ttgaagcaac tgtttgaagg ctgccctttg ggcagcttac
     5101 ctgaatgccc ctccctacca taggtcattc aggtaagctt ccccggttga cagaccgttt
     5161 cttctgtctg cgtatagccg tataaccagt gcgcgcaatt attcgtaatt gttacggttt
     5221 tattactatc cagaccatta tctgattact tcgtcggtgc ggactaggta atcattagcc
     5281 agggtaatcc ccatttgaac ctgctttgca ggttctctaa gacgttttga tatttgcaga
     5341 acggctcaga gaatctgtat ttcaagagtt ttatacagct gacagactgt tttttctgtc
     5401 attgcatagc cgttgattgc aagcctgttt caggtacggt acagctgctt ttttctgaag
     5461 cagatttctt ttaacccatc ggggtacact tccccgaatg ggcatggtgt accctttatt
     5521 ttggagtatt accatgaatc taaatctatc ttttcaccgt tcaggtgtca tccttccccc
     5581 aaatttacta ccggaaagaa tacgtatcgg tgacatcctc acccaccgac atactcatcg
     5641 gaaagttgtc gtctcatgca tacaagaaca tcatttgtta cttgttgatg cggatggccg
     5701 tatttcgaaa atacgaaccc aaaaggcggt caatcggtat tgtaggagcg tcaacgatgt
     5761 tcatagccat aaaaacgctt ccgtagcgtt gaatatggct attcgggcat tggataacga
     5821 caaacgtatt tttacaagat tgggactgcg tatgtcccaa aaagtatatt tggataagat
     5881 ttacagtgct attaagcgct aatttgtttt ttcccaacca cgggatatct gcccgcggcc
     5941 ggtatccctt ttcttttgga gtgataccat gtccatcaat ctttccggca ccttgacggt
     6001 gcaagtcata cagggtcgcc gaggcccttt caaaacagct aaattagcta catcaattgg
     6061 aacatttgat gtaaaaaaac caatattgaa acagtttgag cctggtgaat accaaggaaa
     6121 cttcattatt gaacagctca aagttaaggc ttatgagtgg ggtggtggcg ccacctctta
     6181 cttagatgca gctcttgact ggcaacatct ggcggaattt gcgcaggaat tttcttccgg
     6241 gtcggattct ccgcaagatg atccatccga tccgttcgat ccggttcctg aaaacggcat
     6301 ccggttgcct gaatacgcgg aaactgcgga tcaggtcggc gaaatgatcg cccaaaaccg
     6361 ggagcatatc agtttgggtg agtatatcct ttcagaccgt gacgagctga acatagcccg
     6421 ttcaatgatg aaggaggcag ggtattccta tcggccccag tcgcagactt ggtgtctgca
     6481 ataggaggtt ggaatgcttt acctgacttc ccgcaatgat ttgatggccg atgccttctt
     6541 catttggaac cgacagttga tgtttgcttc tctgcacggc cgcgatgcag acatgttgtc
     6601 cttccaagct caactgcaag taagtaatga acggcttggt tttagacggc cggaggaggt
     6661 tttaagctgt ccgcgcctga cgactgccga gcattgcttg aatctgagca aatacatgac
     6721 gaaatatcag actcttaatt atggttcggt cacgcacatg tttttgtatt cggatattct
     6781 gattcaacct aacttcgata gtaaaacagg ttgggttatg ttggatgatg ttaccgcaga
     6841 cttggacaag gcagcttggc aactggtgcg gcaactctcc gatataccgt tattggaaca
     6901 ttggcagaat gctgttttat cggtacttga ggctgataaa tccatcatgc ggtttactcc
     6961 tcgtatagat gaagagtacg cagtagttgg agttcaagcg gtacgagttg atatcccgga
     7021 agattttgat gtgcgtttga ccgctatgct acaaagcggc cgcttacata cttaatctga
     7081 agcccctagc gggctttttc agacggcctt gcaaaggctc tctgaaaaag tctgttacat
     7141 aagagtttta cacagatgac ggactgtata ttccgttgat ggatattgct gctgaggtta
     7201 tccggccgta ttcgtatggt tgcggcattt ttcaatttta ccaaccctgt ggggatatac
     7261 gtcccatggg gaggcatatt cccaaactta ggaggatttt atgcctactc tatctgaaaa
     7321 gcatgaggta ctgtccggct tgaaccgtca ggatgcagct gctatagtag ccttaatcga
     7381 tttgaaaacg gaggacgaca tggaaaaggt tttgaacaaa ctggatttca tgacccagaa
     7441 cttcgatgcc cgactggacg cttttgaaaa aagtgtagat gcccgcatca gctcatttga
     7501 aaaaagtgta gatgcccgta tcagctcatt tgaaaaaagt gtagatgcac ggtttagctc
     7561 atttgaaaat agtgtagatg cccgcatcag ctcatttgaa aatagcgtag atgcacggtt
     7621 tagcgcaatc gatactaaaa tcaatgcaat ttattggttt atcggtatca cgactaccgt
     7681 tgccgttgct gccgcgaagt tcctgtaaca ttaaacccgt tccgttaaac ggagcttttt
     7741 atctgtaacc ttcaatccca ccatgttccg gcatccggtg ggatttttat catttctcac
     7801 tcaaccctat ggggatatac atccctatgg agatcgtata tctccgttac tttggagcat
     7861 gtgatgtttt ctacaaaata ccctttcata tcttgttttg tacggtaatc agccgtacaa
     7921 tctagaaata atttaggagt atgttcatga ctgccacata tgccgattgc cgttttgaag
     7981 caagaatcac ttcagatacc cgtgctttac ttaaacacgc tgcggaactt caaggacgca
     8041 gtatgtccga atttgtcatc agtgctgccc gcgaggctgc ccaaaaagca gttgccgatg
     8101 cggagattct gatgctgtcc gaacgtgacc aacttcagtt tgcccaagcc ctgctcaatc
     8161 cgtccaaacc gaatggggca ttgagcagag cattggaaaa acatgacgaa ttgttcggga
     8221 gataaaatgc cgttaccgga cttccgaatc cgcctccttg attctgccga tgaccgcaat
     8281 gcatttgagc gcccttccga acctttaaac cgctatctgc aaaaacaggt ttcgcaagac
     8341 gtaaaacgga aagtcgctac ttgttttgtc gcggttacat ccgacggtca aattcttggc
     8401 tactatacat tggctgcaac aggagttgcc ctaagtaata tacccgccgg ttttcgtaag
     8461 aagctgccca gataccctac ggttcccgca gtgctgatgg gccgtttggc ggtagatgga
     8521 aaatatggcg ggtttggact gggttcttta cttttggctg atgcactcaa acgtatcggt
     8581 aaagcagaaa tcgccgcgta tgcgttgatt gtggaggcaa aagacgaagc tgccatcggc
     8641 ttttacaggc atttcggctt tacttctttc gaagatgcca ccaagcatct attttttcct
     8701 ttggcgaatc ttcgctaacc cctttttccg caccatacgg tagcggtttc taaccctgac
     8761 ggggcaatcc cccgtcgccg gggtttgctc cgtctttttt ttaggagcat acgatgtata
     8821 accccgtatc aaaaatagac gaactcctcc cggattttgc agggctggat caaactgtga
     8881 ccgagatgca ggaacgtcag aatcaaataa ctactgattt caactttatc atccgccaag
     8941 tggctgagac tgcccaaaaa agtacggaag ctgccgtatt gccggaggaa tctccggaaa
     9001 ctgaactgcc ggattccgac atacccctga cacagtttct tgccgagttt ggggatagtc
     9061 tctttaacgc agttactgag caaaaccaac cggtctatca gggacaaact tatcctctcc
     9121 ttgaagaagt gctggataac ctggaacgcc ctttgtttgc ggcgcagcgc gaagttgtgc
     9181 gcgccgtatt acagcaattg gtggcagagg acaaacccgc agccattatt aatgccgaaa
     9241 tgggcacagg taagaccatg atgtcggttg cagcagccgc tgccgcgcat caagcaggtt
     9301 tgcaccgcac cttggtattg agtcctcctc atttggtgta caagtggaga cgagagatcc
     9361 tcaaaacggt acccaatgcc cgcgtatgga ttcttaacgg agccgataca ttggcaaaac
     9421 tgttgcaaat ccgtgccctt ggccgcaaac ccgaagttcc ggagtttttt gtgatggggc
     9481 gtgttcggat gcgcatgggc taccattgga ggccttcgta tgccgttcgc cgccgccgga
     9541 taccttctga aggtgcttct gagggttttg ccgtttccga gtattactgt cctaaatgtg
     9601 gctgtgaaat cttggatgag gaaaacaagc cttataacac tgaaggtggt gtgaaaaacg
     9661 ccctggctaa aaaccgccgt ttttgcaaag gcaatcatca tggcaaaccg tgtaaggaac
     9721 cgctctggac cttgtgccgc aaggaacaga gtgaagacgg ggttgtaagc acagtgtacg
     9781 atcgcgtact taaaaccatg accaccctac ccggcatcgg tcctaaaacc gcagcaaaaa
     9841 tcatcgggat gtatggtgag cagtatctgg ctgaaatctt ggaaaacaac attcaggcgt
     9901 tttccaattt gatggatgaa aacggagaat ttttattcga cgaccgtcag gcggcgcgta
     9961 tcgaccgaga cttaggcaaa gcagaattca gcctgggacg aggcgggtat cagccgaccg
    10021 agttcgtcaa acgctattta ccgaagaact ttttcgggct gttggtagta gatgaaggtc
    10081 acgagtacaa gaactacggt acggctcaag gtcaagccat gggcgttttg gcacgttgct
    10141 gtaataagat cctgtgctta accggtacgc tgatgggtgg ttatgcggaa gacctgttct
    10201 ttctattgtg gcggctttgg ccgcaaatga tgattgaaga cggtttcagt tacaacaaag
    10261 ggaatacctt gggttcggca agcatggcgt ttatgcgtaa gcatggcgtt ttgaaggata
    10321 ttgtccgaca tcttggaact gaatacagta atggcgcatt cagtagctca aaagcagagc
    10381 gtaacagcgt tcgcaccgcc aaggcgccgg gattttcccc tttgggcatc atgcgttatg
    10441 ttttgcctat taccgtattt cttaagctgc gtgatttggg agaaggcgtt ttacctggct
    10501 ataaagaagt tttccggccg gttgaaatga ctgaagatca acaggcggtc tataaaaata
    10561 tggagagtgt tttgaggcag catttgaagg tggcattaag gaatcgtgac aataccctta
    10621 ccggcgttgt tatgagcgcc ttgctgtcat ggccggattg ttgttacaaa cctcaagatg
    10681 tttactggcg cagacaaaac aagattcttt tccataccga accccagttc gatgaagaaa
    10741 aaatctcacc gaaagaagct gacatgctgg aagtcgttaa agacagtctg gctaaaggac
    10801 gtaaatgtct ggtctatact gtttataccg atacccgtga tacgacaggc cgtctgaaag
    10861 agattttcaa acggcacggt attaaggcgg cggtaatgaa agcgacggtc aaagccgatc
    10921 aacgcgaaga ctgggttgaa gaccaattgg cttcaggcgt acaggttgtt atctgtaatc
    10981 cggagctggt caaaaccggt ttggacttgt tggcatttcc aaccatctac tttttgcaaa
    11041 caggatataa cgtttatacc ctgatgcagg ctgcccgccg ttcttggcgt attggtcaga
    11101 aggaagacgt ggaagtgtat ttcgccggtt acagcgatac agcgcagcaa atttgccttg
    11161 aactgatggg gcaaaaagtt gccgttactc agtcaacctc cggtgatatg cccgattcgg
    11221 gtctggacat tctgaatcag gctgaagatt cagttgaagt tcagttggcc aaacgcttcg
    11281 ttgaccgaca agattgatgc aatttttgca tctaacccgc gtttagtggg ttttttcaga
    11341 cgacctcgtg ttgaggtctt ctgaaaaagc ccgtaaggtg gaatttcaca ggctggcaga
    11401 ccttatctgc ccgcgcactg ccgttaccca ttgcgcacgt ccttcgggac ggacggctct
    11461 ttttcaactt tctttaaccc tgtggggata taccgtatcc ccacagggca tcggtatatc
    11521 tccctttttc atggagatac cgatgtgaaa ttttttatat tcatcaaacc cgaccttcct
    11581 tttcacaagg aaagcattga atcttggatc aaattgctgg aagatatagg taaaacagcc
    11641 ttgttggcaa tgctgccgtt gttttggctc aattcatact ctttgaccga acgtgcttcc
    11701 ggcgtagccg tattggcttt ggttgcctac tttgtgcaag tatatgctga caaactacgg
    11761 aaataccgtg attcattaaa cagaaaggat acgtcatgct gataggcggt ctgctattta
    11821 ttctcttcct ggtgcttgct ttcggctatt ggattaccaa gcagcttgaa acttcgcgta
    11881 cgtcggaaga acacaaaaac cgttaacgcg gttcattcta tgtagctcaa gtatttatct
    11941 tttacttgga taaatatgaa tcaaagattg tcaaacagga acccaaggaa agggaaaaac
    12001 catgatcatg acctacagct tactattttt gattgccgtc atgttgtttg gcatatttaa
    12061 actggtaaag tttggcgagt ctcagcaaga cactaataca catgaccggt caagttaatt
    12121 ggtattccca tcttttaaat gctttgttgc tatttaatac caaagcattt taaatagaac
    12181 gctgatacgt tcctttttag ataaatccgg cttgaagccg caatcccgct gttccgtacg
    12241 tctgtacggc agtgggattt ttcttggaag tcatgcggtt acgttttttt taatcgtatg
    12301 cgtgtatttt taaccatttg caggaggaaa acctattttt gtatcggaga agaaatgaga
    12361 tatagcaaaa acaaagatta tcaatttttt atacgccaac tggtatcggg gggagaatgg
    12421 atgttcctcc ccaaaaacgg tcgtaagcac agcgcattga agcacctacc gaccgatcga
    12481 aaaattccga tacccggctc gccgggacag gatccgcgtg gattgctgaa ttttaaaaca
    12541 atggtacgtc atatagaacg tggcggtacc ttcgattaaa ctttgattta accctgcggg
    12601 gactgtcccg cacggcatgt ctccgttttt tatttgaatg gagaccaata tggaaactca
    12661 aaaccatctg atgcaccccc gtgtcgcgca taaccaccgt gacaacggat acttccccac
    12721 cgaccaggcc acattgtcgc gtatagccgc ttgccttgat attccggcat ctgccggagg
    12781 tttgttacgc atatttgatc cttgctgcgg aacaggagaa gccctggctt ctttagcttc
    12841 tactttgcaa tcggaaaaaa taagttgtca aacttacggg attgaactgg agaaagaccg
    12901 tgcccaagag gcaatgagcc gactgaccag tgtcatcaaa tccgatgtag aaaactgcac
    12961 catgcagggt tcggcagtcg gcctcctgtt tctgaatcct ccttacggct ttgccgccaa
    13021 agacaatctg tctaatcaaa gaaccaaacg gcttgaagaa cttttcttcg cacgtacctt
    13081 cggtactttg cagcccggcg gagtgttggt aatgattgtt ccggaagccg ccctgacaga
    13141 gcatctaacc ggcgaaatcg ctacccactg tacggatgtc cgtatgataa gggctgcggc
    13201 ggatacctac aaacagttgg ttatattcgg catccgaccg aagaacaaag cctctatcag
    13261 caaaaaacag gcggatgccc aacaaaggct cctgcttgat tttgaaaatg cccctgagac
    13321 tttgccggcc ggtatgcctg catactgtgt gcctattgca cccaataagg tctttcgccc
    13381 cagaagcttt aaattggagc aggatatatt ggaacgtgaa ctgggacagc agcaatcccg
    13441 cacgttatgg ccatcatttt cgcaatattt cggcaccagt gccgttatag ccgaaaaacg
    13501 ccggccttta tgtgctctgg gtcagtggca tgctgcttta gcattagccg cagggcaagt
    13561 taatggcgtt gtcacctcaa ccagtggccg aaagctgctg gttaaaggtt caacccataa
    13621 aaccaaagtg gaaaccaaat cggaagagta cggcaagaag gacaggcttg tggtcacgat
    13681 tacttcaact gaccggtttt ctccaagcat acgcgcaatt gatttgaccg aaggatcgtc
    13741 ggattttgga cgagtcctga caatcaaata gattggccta ttcaattttt aaaccctgcc
    13801 gggtacttcc cgcaggggtg tatccggttt ttttaaggat acgcctaatg gaacaacttg
    13861 attcggcagc tccggccgca tgtgataccc cctctctgtt tgacctgggc cgagtgtcta
    13921 tgacggcaac agtagattac cgggcaaaag agcacctgcc tgcacaactg tatgagaaca
    13981 gtctttatgc ccaaatattg cttgaggccc accgccatgg catatggggt gatattcctc
    14041 ctgaggatgc caagccgaat cagctggcac tcaagccagg tgaagaaggc agaataatgt
    14101 cttcttacaa gattggggac caaaaaattt gggttgttac agaatgggac agaagcctta
    14161 ccacactcct gttcccagaa gattactgac cttgtaatct ttttatatat ctcacctgcc
    14221 ttcgggcagg tattttcaga cggctttagt aaggccgtct gaaaatacct gtctttacag
    14281 gctggcagac cttatctgtc ttgtgtcctg ccgttaaaca tgacacgcat ccttatggat
    14341 gtacggcatt ttcttttatc aaccccgtgg ggaacacaat ccctacggac tgtgttctcc
    14401 gttttctttt taaggagcaa cacaatgcaa aactttattg tatggatgaa tcccgatcgg
    14461 gaagaatcgg cagctgcggc agctaaattg aacggtgcag ttttgaccgc tcacacggat
    14521 ggtgtcatgg ccgctattag acgtgaactg aaaagatttg gattaggggc tgatttggcc
    14581 gtctttgccc aaacctgtga agagtttgcc aaacaggcta atgccgacat ggtttgcatc
    14641 agcagtagca atggtgatgc cgttgcagcc gtcacttacc ttgaggacgg atacgccggt
    14701 aaaggcgtat ccgccgttgc cgacatgcgc tatctgatgc ctgcctatcg gcagtatttc
    14761 aaccgtcctg acaaaatggt tatttttcag ggggcattgc agtatttatc agatgaagac
    14821 attgcctgga ttttccgccg ccacatcaat gccatcatcg agtggcagtc gaatgaaggg
    14881 ctggggctgt acgagttcgg tttcagtcta tctgatgttc tgactgatgg ctgccgccat
    14941 gtgctgcgcg aagtcgtcac cggctggctg aaccgcgaaa tcctgcccga gctgtacaat
    15001 gtgtaggcat aaccgccgtg cttcttttcc aactgggtaa atgcccatta cctgcggtct
    15061 tttggccgca ggggcagaaa ctctactgga gatagttatg tctatcaaag ttgacaacat
    15121 ccttctaata gaaggaacca aagaatcggt tgccgaagcg gcgaaactga ttttatttcg
    15181 ctcccccgtt catcctgagc attgggcttt tgatttcaat cggattttgc ctttacccga
    15241 aaatatcacg tctgaccttg ccaaacagag aatcgaggca tggggcagca gtttttcccc
    15301 atatcccacc gaaacctttg ttacacaaag agagggtttt attgaaatct gctttttaac
    15361 cggattggca ccgcctttcg gtatttatcg caaactcgcg gaaatcttcg ccaacttgga
    15421 tgtatgtttt acagcgaaat acatcaacca gtacggaagt ttcggcggac gctacgaata
    15481 ccgtaacagc gtgctttacc actgtctttg cgagatggcc gacatccgta aattcggtat
    15541 cgccgaattc gatatttggt acgaaggctt gtccgataga cttgaaagcg acgggtattt
    15601 ggtagaagat tagactatgt tacacggtag gcagtccgtc aaaccgcccg cataccgccg
    15661 ttaaccaaat gcacatccgt ttcggatgca cggctctttt atcaaccctg aaggggatgt
    15721 attccccgtc atcggtgtgg tggtctggag taacggatat agtgaggaga tgaagaatct
    15781 tgccatggca ggattggtgc tcctgtcgtt tgaaatgctg tatgtttcat tccttatccg
    15841 cattcacatc tctaaactgg aggacaaata atggacttcg ttaccggaac ttgggtcatg
    15901 gccgcagtcg gcctggttct tgttattgtt gcgagaatct atcttaaaac ccccaaaaaa
    15961 taaccgtgct taagcacatt tttcatcaag ccggcggaac cgtcgttccg cttcccaaag
    16021 gcaaagttac cgtttcggtt tctttgcctt ttatctaaag cacctgtcgt ctgaaatgtg
    16081 cgccggtttc cggtggcggc ttttttatca acccgggcgc aagcccatca acctgcgggt
    16141 ttccttatcc gcagggatgg ggattcctgc ttaggagact actatgccca actggtgcgg
    16201 caacagcctt cattttcaag gcaatcctca acgcatcgcc gaagtggcgg agcaaattct
    16261 gatcaaaacc gacaaggggt atcaattcag ctttcagcat attgtaccca tgccggagga
    16321 gctgattctt cctgaagtgc ctgatagtta catgcagcag acctggtttc aaattgctca
    16381 acaacctgaa aagaagttga tcaatttgga acgggaaatg ctggaacagc gcgataaatc
    16441 cttttggtcc gttcatcctt ttggtccgtt caatatagat tgccggatga acttttctct
    16501 gaattaaagc tatatttttc ggaaaaattt gattgggaca atctgactgt tggtgaagta
    16561 tttctccatt ttgaagctaa ctctgaagtc gcatctcgtt tccgttacga tggaccattt
    16621 gccaagcgta ttgcggaaaa catccgccaa tacggtcatc cgaattggta tgactggcgg
    16681 ttagctaact ggggctgcaa atgggacgta aaccccgact gcacttttgt gacagttggg
    16741 gaaagcggca tccggatcag ctgtgacact gcctggggtc ctccggaagg gatttataga
    16801 gaactcgcca aacgttttcc tgacgtagaa tttgaagcca aatacctgga agaaggaatg
    16861 tggtttgcag gaacctatga aggtcatgaa ggagcattgt tcgattatcc gtgcacagat
    16921 gatggagtcc gtgacttcgc taccgagcac tttggatgcg aatacgatga cgaagattaa
    16981 agaacttaga tttcgttaga cgtattgcca actcaccctg cggggacaac ccgcagggtt
    17041 gtcccccgct tttttgtgga gattagaaat ggttgaaaat cagattgaca aagagatcac
    17101 tcaggcttca tgcgaaggcc gattcatcct aaaacaggaa aacggcaaac gtttccttta
    17161 ccttaacctg cccgaaggta gtgacgaatt aaataccatt tggcagacgg acgagtatga
    17221 ctttaccgta cccgacttag aagtcagcat cgatgttgaa tccctttaca ctgctgttag
    17281 acttttgaac gaaaaccagg gcattttgca cggcatcagc acaaaatgca gtgcgtattc
    17341 gttcggtttc gaaggaaagt tgagatacga aagattggat gtaaagcctt tccctatcaa
    17401 atcgttctct tattatttgg agttttataa cgactggacg gggacgttgt atgaactgga
    17461 cttatcagcg ttcctggatg agtttttcgg agagtgtgat cctgaatcca ggctcgatgc
    17521 ctgtttgaag tagcatttgt gcctcaaata atctttttca aaaccaacgg ccgctaatgc
    17581 ggccgttttt ttgtgcccat actctgatat ttttggggtc tctgccccca atcccccggt
    17641 ctcgccgccg acagctcggg aacaggaatt acctccggcc tacgcctgct ccctcactta
    17701 agcgtgtcat cgctgccagt cagtaaatgg gtattcataa acgcccttgc aggtcgaagg
    17761 ggttgggtat tgaccctgta aggaaatttc cttacaataa acagaattct ttaatttaag
    17821 cagtttttaa caatatcaat aatttcccaa gccgttaagt agaagatgtg cgtaactagg
    17881 acattgaagt cgcaagtagc aacagtaaat ttgaatgaag gcagggcaaa gagaccatga
    17941 agattatttt tgatgcagtg tcaactatga ccagcaaaaa ccaaacaacc attcccgagt
    18001 cagtacgcaa ggcgttgggg ctggaaaaac aggacaaaat ccgtttttcc gtcttaggcg
    18061 gcggacaggt attgctagaa aaaaacaata ctgaggaaga agaatttgag catgatccag
    18121 ttgttggaaa attcctgcat tttttggaaa attctatgac aaaaaatccg aattccatca
    18181 aacccacatc tcaatcacgc tttaaccgcc ttcgccaact gacttgggaa acagatatga
    18241 aagactaata tgcctgattc ccctatgcag ataaacggat ataccattta cgaacacgaa
    18301 tgctttacgc ggcaattaga cagactggcc actacgatag aaagccagaa acgatccgaa
    18361 aaatccagta gatccgaaga ggccaaactg ctggaacgaa tcttaggcga aatagaaaat
    18421 attgccctta atccggatag cccggaatat cggcttggca agacactcgg cccggaatac
    18481 aaattttggc gacgtgccaa gtttatgggt agataccgat tgtttttccg ttacgactca
    18541 aacagtaaaa tcatcattct cgcatgggta aacgactcgg gtacgaaaag aacttatggc
    18601 ggcaaaaatg atgcatatac cgtgttcaaa aaaatgctgg ggaacggaaa tcctccttca
    18661 gattggactg cactgcttga agtagtatca aaggaataga gcaaaagaaa acagccctta
    18721 gaagattctc atggtcaaaa tacagtatta gactgaatgt tacaacattg taataataat
    18781 tgctgttatc tataatgttg cccatgtccg tatatgagcc ggacaaatct aaaaggggga
    18841 agaaaaattg ccctttggcg gcaaacagga tatataggat tttattgttg tcttattctg
    18901 atgatgcttt tcttcccaaa ggattttaat cgtttgaaaa ttattgctta tacgagtccg
    18961 gatggtgatg aatgggtaac tgcgctggaa gcaaagctaa aacaccggga aggaatgatc
    19021 tactacagcc gtcatcgtcc cggtacaaga aagaaaatgg ttttgaccag gaggaaggcg
    19081 accaattttt ttcgctatta cagtgaagcc gactccggtg gtgcttccgc ccctgaatcg
    19141 ctaacccact tgctttgcaa acaagtgctg aacgaactat ccaacttacc cggcggcctg
    19201 acgactgttc taaattgcac agaacacgcc gagcaacaac cgcctgtcac tattaggctg
    19261 aaccgtgcat tatccgaata tcggatagat atagatggca aaacttttta tattgatgtt
    19321 ttgcttgaat ttgatcagcc gggtaacact tccctgctca ggcacgagat aaggtggcag
    19381 agaaaattgg cggttgagat ttggcataca tcacggttgg cttccaatgc gcctaaatgt
    19441 ttagccttat cgaaaatagg tatcccggtg gttcagatac gggcagataa gggttcattt
    19501 ttgtatattg atgaagacga gctgctcaat tatgataatg aagaaatcaa aaaccgcata
    19561 aatcgccatg tagagaaact tcggaacacc tttcggaaac aaattctctg caccctactc
    19621 cgtaatcctt tgagtgcgga ttttcagacg gccttgatgt tgcataatca aattaaggcc
    19681 gatgaacaac aagcagagca aatacaggaa aaatttgagg cgctgaggaa taaacatgtt
    19741 ttattggaag ctgaatattc ggctttggcc gcccaatacg cagccttatt agaacatcag
    19801 aatttccaag ctcactccgg gaaaagggaa attccgaaaa agcatgggat tctgcagcgc
    19861 atggccagtt ggtttaaatc ataaaccact gtttagaaca aaccctaagt ttcaataaga
    19921 tgaggaaaat attgcaatga aaaaaattat gttcggatta ttagcagcta tggccatttc
    19981 aaatgcccaa gctgaaggta atttcttgaa tcttgcttca aagccggcca aaattgacgg
    20041 ggtattggaa gcttctttag acaaacccta tcccaagttt gtacaagatt ggatgaaaga
    20101 cagtaaattg gcgggacttg gccgtatcat ccaaggcaag atttttgaaa gtacgggaac
    20161 tattgaacga aaattctatg caagatccga atccgaacat gaaaaaacgg ttgaggatgc
    20221 agtaggaact ttcaaagcat attgcgaaca tcatcagggg acggttgaaa ccctgacttc
    20281 cgatattttc tgtatcaacg gcagacaagt ccagtggatg ggatggtggt acagagaaaa
    20341 ctatacgaat tttgcattct ctgatggata tacaggggaa caagcctacc gcagcaaagt
    20401 tacggatgta caacccggta aaaaaatctc cacttcctat gggtctgcct tggtcttaga
    20461 aaaaggtgag aatggcttaa ttaaaattca gccgaacaga ggcagcgaac gttggattac
    20521 aagtgacagc atccaagtaa cttataaata atgggatgtg tatcatataa gaaagaggat
    20581 tggtagctga aatgctgcca atcacatgga gtgcaaattg aaaacaaaat ctaaaaaact
    20641 tttgaggctt gttatcttat ttgtagcggc attctccatc tctacacctg cggtaatgta
    20701 cttcagcaca ggcgaagcga cattaaatct ttttgttgag gggttttgga taattggatt
    20761 acttcaagca ctcgctttta ccaccattgt agactattgc actgaatcta aacatacaaa
    20821 tatgctcaat aatccagcgt gcaacgatga agattgggat aatgatatgc tcaataatcc
    20881 agcgtgcaac gatgaagatt gggataatga tatgctcaat aatccagcgt actcttggtc
    20941 aatgttcaat attcaccatt cagattaaaa aagcaggagc cttatactcc tgctttccgc
    21001 agcaaagtta cggatgtaca acccggtaaa aaaatctcca cttcctatgg gcctgccttg
    21061 gtcttagaaa aaggtgagaa tggcttaatt aaaattcagc caagcagagg caatgaacgt
    21121 tggattacaa atgatagcat tcaagtaacc tataaataaa ggagaagtat catgatcgga
    21181 ttcgtaaaat ggtatagcta tgagaaagat tttggcttta tctttggtga agatggtcat
    21241 gaatactttt tccgtaaatc cgccggttca gatataccag gacagatagt gaatttcgaa
    21301 ccatttgagc atagtcgtgg gctgcgagcg cgaaaggtta gtaatataca taatcctgat
    21361 cctcaacaga tccaaaaatt gaaaacctta tgcaaagact ttcagaaagt tgaaaagcaa
    21421 cccatgtcta aaaaatacaa cttgctttcg gcagctatat tatcgctgtg tttgactatg
    21481 ttcataacac tgggcttttt ctacatctta gccggctttt ttgatactga tagggagact
    21541 aacatagttt tgctgtttgt cttccctgcg gtaacttttt ccgtgctgac attctttctg
    21601 aataggatat tcaggaatat gaaaccgggg tctgacactg atgttgccac taatgatgat
    21661 tggttaaagg atttctggtc tgaggattca ttaatttatg acccagctta ttccaatctc
    21721 aattgcaata tatttcatcg atcagatgac gatatataag aaagaggatt ggtagctgaa
    21781 atgctgccaa tcataggagt gcaaattgaa aacaaaatct aaaaaacttt tgaggcttgt
    21841 tatcttattt gtagcggcat tctccatctc tacacctgcg gtaatgtact tcagcacagg
    21901 cgaagtgaca ttaaatcttt ttgttgaggg tttttggata attggccttc tccaagcagc
    21961 agtatttacg tttgtggtag actgctcaag tagtgattta ggttcattaa gtcaatggca
    22021 aattggaaat gactgggaca gacaaatgga tccattttac gctcatgaaa ttggtaacat
    22081 ttggcatgga acgcacttag actcgagttc tgaggagtat tggaaataaa aaacaggaga
    22141 tttataaact cctgtttttt tatttccaaa agatggttaa attatctatg tttctagtac
    22201 cttaccaatt gaagtgatga tttgttcgaa agctgatttg ccttcttcgg atactagatc
    22261 gttacctcca ctataactca ttctaacttg ataattccct tcatcaacct cttcaatctg
    22321 aacaacaatt tgtgcaatac tcatctttta aaatcctctc tgcctaaaga aattatgtac
    22381 aaaaccccaa cttcgtttgt gttgccggta ttcattagga gctgttggga agctggccca
    22441 gataccacct aatttcatga ctgcctgctc gtaatcgcca tttaaaatag cttccattgc
    22501 acctttttta gctaaaatta aggcgacagc agctttgtct tgtgtggcgg gactaaaatc
    22561 cgtgaagcca tattgttttg ccagtccttt ccatgtgtat cccatgaatt gatagcggcc
    22621 ggaggcatca gatgtgcttc tactaccatc ggttcgagta aaactacgtc taacagagtt
    22681 acccgggtga cgggagaagt ccgtaattct ataattacct attgccgtct gataaccatg
    22741 ttttacacct tctgatgccg caataaaatc taaaaaggcg cgcacttgcg gattatgaac
    22801 atatcgctcc aattcggctt gattatgagc agttgaagct tttaatacat agtttcctct
    22861 cacatgtctt ccacttgata ttccacgcca cataaccccg ttgcctccat atgaggtggc
    22921 attacgcaac ggggttattc ctttgggcgg aatacagcgg atccgctaga cttcgaattc
    22981 cctggacgtt gaggaattgg taggtcaaat tcggatgcta aagccgggtt gcccaaatgg
    23041 gctgctgctt gatccgaccc agaacctatg tatccttttc cttcacgaac gtctttcatt
    23101 gcattatcgt atctttcacc gaaagagttc cagggtaagg cgtgagtatt aattctcatg
    23161 gttaccccat tcgtttgatc ttgagcattt ttagcatgcg ttgccaatct accaacttct
    23221 tggcggtggc tatttctacc ttgattaata tcgccttcaa ccttatcaga tttatttgcg
    23281 tattcctgtt tcagattctc acccctagcg ttgatagcag cactaattcc atccggaatt
    23341 ataccggcct gatgagcttg ggtttgaaca tctccaaatg ccgccccagc tccggatgaa
    23401 actttatcag agaagtgttt ataagcggct tgcgtattgt tttgaggtct atcttcaggg
    23461 gaaaggtcat taggtttcca gcctgaattt tccgcagcag cttttaaacg atcttgacta
    23521 tttacacctg cacgaattgc agcattgtag gttcctccct gtcctgcgtt taattcactc
    23581 atagtcaatt cggggttgtc ttgtaccaat gcatttgtgt tatcttgggc aatggtaatg
    23641 ccattaccat aagtttgagc tgccatttct cgataagatt gagctttttg gtagctttct
    23701 gatgccatat gcgaggcttc tcttgctttg ctaaagttag cttcggcagt ttgtgccaaa
    23761 ctattaccag aattgagtgc aactttatat gcttctgatt gggaaacttt gtctgtaaaa
    23821 ttactatcaa agctaatacc ttggcgttta cctgcttcaa tactttcccc aatttctgct
    23881 accatttggt ccgtattttg tgatttacca ctccaaccta aatcaccttt tgctcctaaa
    23941 gcatttttaa tattttgggc tttagtacta ctgcccgtat tgccactaat gcctaaccca
    24001 gctgcgatgg cagatataac tgctgatgta ttagtaactc caaatttttg ggcaatcttc
    24061 ttgccttgat ctataacgta agataatttt ctactttcag ctgcatccaa acccattccg
    24121 taaccttcag aggcacttct atccatacca taattggttg caaaggcaag gcggtttgca
    24181 ttgccttgag atacggactc tcgaaaggaa gcttgccatt gttgggcaga tgtttctgct
    24241 cggtctgcag ccttcgaata tgactctgat tggctaatct gtgattgaga ggaaacacca
    24301 aaactactat tcctcatctg cgctctacta tctccagttg ccatattccg ccatgatgtt
    24361 ccggctgcag attgagcagt catcatttga ggatcggtat atacagggtt taagctatgt
    24421 ttgttggcat tgctattgtt gtagttcata ttgccggcag agtaattccc cgaccctact
    24481 tgggaacccg ctgcttcacc ggttgatttg gccgatgaag tcatgcccat catgccgttc
    24541 atcaggctgc gtccgcccat ggcaagagca cccgcgatga ccggaatcag ccacattgca
    24601 gccccagcag ctgcttgtgc atcggctgct gccatcaata acgtatcacc tgttcccggt
    24661 gtaataacgc cgttggcggc ttccacgcta aacagacgct tcgcattcat agtcataatc
    24721 atattgatga tactggctac gggtgcccaa agctgaatcc atagcatact catcatgtag
    24781 aagcttaata accggaatgc catgatgcca cccaacatgg cgagaatcat tacaaagggg
    24841 aataaaccaa tcagaatggc ctcagcagca gaacgaatca agggaagatt ccaacttgcc
    24901 attgatgagc cggttttttg ggccgcaata tactgattac gagcttgaac ttgagcaaat
    24961 gcctgctgta cggcagccgg atttcggttg cgttggccat tgataatgcc tgcttgacga
    25021 ataacctcaa ccatcgccgc ctgttcgata tttttcaatg tatcgaagcg ggcattatcc
    25081 agcagatatt ggaatgagct attgcctaag gtaaccaggg catttacccg gtttattcct
    25141 gccgaatttg tacccaaacc tgcaaaaaca cgatcggcaa tgtagccggg caagtaattt
    25201 ttgctataaa accgggtacg gtcttttagt tcttttgccg cctcattgca ggtcatggtc
    25261 tttgatttgc cgttatactg caacatgttg cctcttcttg aggcgatctg cgatacattg
    25321 gtaaacaagg cttggttggt atgggtcaat gtagccagaa tatcggcact ctgtgccaga
    25381 tcctgcacgt tatagaagtg atacaggttc atatcaaaga atgaacactg atacatgaac
    25441 agtgcccagt cttgagtcag ttgcgcgtcc gggattcgtg cttcacggat attttccatc
    25501 aggcgcgttc cgaacagcat gccggtgcgt gagtaattga ggtcgtccgg caggtggtta
    25561 acctgctcgg ctttgcgggt aatactttgg ccaaaagaac tgacaaagta gcccatataa
    25621 gcaaggccga ctggtacatt gccgacagta tattgacggc ctgtatatcc atacccacct
    25681 gattcagtaa tgagtacagt ggttttaggg accatcatac ccatccaaca aaataacacg
    25741 acaaacaaat aactgaagtt atccttcaaa tccaatttga agactgcaac agcaagagtg
    25801 accaggaatc caaataaggc agcagcctgc attgcgccac ccatcatcgt agatcctgat
    25861 attgtggcaa cgccattgaa aaccgcccgt acagcttctc cgttaccagg gaaagtaaag
    25921 tattcgacag ccatcatctc tccttagaaa cggttcatcg aatcaaaagt catggagttg
    25981 ttggtatcca caaaggctga gcgccattgt gtttcaaact gctccatagc cctcatcatg
    26041 ctttcttcga tctcgatttt cttcattgag tcaatgcgct cctggtaaat tctgcttcgc
    26101 aattcgccaa ggttggcaaa caattttcga accgcttccc cgctggcatc gtctgtcttg
    26161 ttatacatac ccaaggtcga gcgtacctgg cgttccgatt ggtccaaaat attggccata
    26221 atatcaacgg caatcgcatc taaaaccgct gtttttttca aatagccagt tccaaggaaa
    26281 gcatctgata cagccatttt cagtatgggc aatttactgt tattgacaat ggcagcaatc
    26341 tgcttgcgcg tagataaatt ccaaccttgg ttattcctta ttgcagtcgg aagcgtttca
    26401 atgcgtttgt gaaccatgta ccgcaaagaa ggtatttcta catcattttt ctcagtgcag
    26461 ttctccatta atttcttaga accgtctttg cacgtccaca tatccaatac aaccttctcc
    26521 cggatttctc ccccggcatt gttttttccg tttaaaagat cttggatgct gaggattttt
    26581 ggttcgaaac tttgaatacc tactgtgtat ttgtccttat ccccttcatg ttgtgttaat
    26641 ggagtgaaca caattgttcc cagcatgctg taatagaagt ccatgtcgat acccagtttc
    26701 gagcgttgac ccgtatagtt cttcatggcc tcataagtca aattaccgcc ggtaaagcta
    26761 atcgtatctt ggggttttcc ggtgtttcgc accttatcgg cttcatggac aaggttttta
    26821 gcggcctgac agtaccaagc accttctgcc ggatcatttg acgaacccga atccactgaa
    26881 gcggcgcgac aatttgaact ggccaacgaa cccagtttcc ccattgcgcc gccgacaata
    26941 tcctgactca gtttgcaact gtttaaactg ttttgattaa cataattgac caaatcacga
    27001 ataccgccaa ttgcatcttg gatcatcgga tcaagggcct taagtgccag gtcaaaggct
    27061 acacctgctg cgttattgcc caggtttcgc gtaaattgga tgaattgttc tttattgaca
    27121 aacgagaaag agccggcaaa gaaatctata ccgccgcaac cggcactgat tgaaggtaat
    27181 tgaatattcc caaggctgat atccgacacc ggaaagcgaa cgctcatacc gcctcccgta
    27241 tataggctgc cgctttgacc gtggaacgca cctgcttcag tataattggc ttccgctccc
    27301 attgagttaa acatgtcagc catgttcttc tctatgccgg cactactatt gatcggaagg
    27361 gccagcgatg ccgccaagca tagatatttt gcagaggaag cacaaactgt cggcactttg
    27421 gccggcttcg gaaatcgaaa gatgtgaaat ttcataaccg ctccatgaag cggttatagc
    27481 agggaaatcc ttactataac caagataagt tacagtaaag acttgagatt aagggaatct
    27541 tattttacct gaaatatccg cgttgtcaac cggttaatac atagaaatat aaatattgaa
    27601 ggccccgtat agggcctttg atttgattaa tacaatgtcc cgacagggcg ggaatacgct
    27661 aagaaacgat cctcaagcgt ggtttgcgcc atcaccccaa atccaatggg tgtaaattgc
    27721 ttggtttttg tatccatgac gtacattgca ggtacagtag taatattcaa cctttgggca
    27781 ataccgttgt tcggcaaagc acgcgggaag tgattaatac ctccgccatc gagactgacc
    27841 ggcataacct taataccgta ttggtcggca aagtttttca aaatcggcgc catggtatga
    27901 cagtaagggc aatccgattt gaaaacaaac ataatgccgt tctctccggc aagttttcta
    27961 attgccccgg ccttttgttg ctgacggtta tgatcatatt gttgctgggc cagttggttg
    28021 ttcggacgac cgcgctggct gtaatccaaa tttgggtttt gccaaattac cctttgccat
    28081 tgatcggcaa atgccgcaga tttgttcatc acaacctctt gaagttcaat atatcgcttc
    28141 aaattttgag gtgtggggtt catgacggca attttacgcg actcatccaa atttctttgc
    28201 agcatttcaa attctgcaag ttcattacga accggagtat tttgaggccg gttttgtccg
    28261 gcttgtttgg gctttttagc atccggatac cataagaaag cacggttttc gtttttccag
    28321 atatcttggt cataccatcc catgccatca tccgcagctc gcagattgga ggcgtgcagg
    28381 ctaaatatca gcatgacggc catccgcact cttttcattt tttaccaccg ttctttctgt
    28441 tcgggcacgc cccgatatcc ataatttcag acggaaaggt ttgcgacagt gcgggcgaag
    28501 tatggaatgt caagcgaacc ctgttgcatc cgggtttgtt tttaatttct tccattctga
    28561 ccgcctcgac aaaaataggt atgtcctgcc ggaatttctg tttgacggca gccgcagcac
    28621 ggccgctgaa ataccctttt gccgaaccgt gacgcattgc cagcgcaagc aaggcatccg
    28681 gcttgccgtc aggaagcggg atggaagtcc ttgctagagc ttctgttttt tccaaagcga
    28741 taccgccggt ataaggaagg gatgcggcaa gtgccatacc tgtaaaaaac atacccgata
    28801 caatacccgc cgacgcagcc aaccaattat tgatattcat aatagttctt cacttccaaa
    28861 tttttcccat taacggatgc ctccctgatt ttttgtgaaa ccttttgccc ctgcttggcc
    28921 acgttgtcca tgttggcata gatttcttca taaaacccgg acagatcaag tttggaaaag
    28981 tctaactgtt ccaattcctg tgcggtaaat ccttcgcatt tcgggttctc cggcgtaccc
    29041 caaccccgtt tcaattgctc tttgccgccg acattgacta ttttggctaa tttggaaatg
    29101 aaacagcact ggctctctat cattgttaca catgccccca atattttgct gccgcaataa
    29161 gaaccgacat aatggcacaa atctgcttta cgcttcatgg caactaaaat ttcactttgc
    29221 gggcacttca accactgttg gatggcgatt acggcaatag cagccgcaaa tacggccggg
    29281 ttgaagccga caattacccc cataccctgt tgtcccgcag cagagaaacc caacgcacca
    29341 atctgagatg ttccggtaat accgccggcc gcactcaggg ccgtacccat tgcctgtgct
    29401 gcggagctga caaacatagt ggtaaacgta taatggctgg caacgctggc cataaaatac
    29461 gttgttgcca tagttgtccc tgtctgtacc gcgaagtcaa caaatttgcc cggatctgct
    29521 tttgttcgac agcagtctcc gccgaatacg ctgtctaaag caccatccag cttgatagag
    29581 cattgcccgg cttctccatt gaaaaaacgc tgtttttcgg gatcataaaa ccgttgcgtc
    29641 tcacgggcga attccatcgt cgtcatagtt ttggcaaaat cccgatcctg acaaccggat
    29701 acctctttgg ttgtctctcc tttttcggta cattcaaact gtttttctct ggcttgacag
    29761 gtaccgtccg gcagctgatg cgtacacttg tccccaatca gacggcattg gtctgaggat
    29821 gtataagcgc tgcatgtttc ggaatcgctt ccgtccgtac attgcacggt gcgcctttct
    29881 ttccagcaat cacgtgtaac gggtacgccg ttgatggtct tggtactgcc gggttccaaa
    29941 cactctgttg caactgcttt acattttttt ccttgtacct gacatgacga ctcatcccat
    30001 tgcgaactaa tcagatgggt atgctccatt tcttcgactt tggtcggaag ccttccttga
    30061 tgtaacgttc tcaaatccac atcgcaagaa tacttgtgcg tgtaacgcaa acactccccc
    30121 tcattcgact gctccaaaca ggtttgtccc tgtacttcac attttgccga agggtctttt
    30181 aacggtgcgc aataatcggc agaatccttc tcaaggcact gatactcttc tttgtattcc
    30241 caacagtctt tagttacgtc gaccccgttg atgtttttgg tcgacgggcc atcaacacaa
    30301 acggaagacg tccgggtaca cgctgcactt tccctgagtg cggcagcaga cgctgaagtg
    30361 attaacataa agtggcagat cataaccagc aaagacgtta tagccctcat tgttttaccc
    30421 tcttctcata ctccgaacaa ctgttttccc atttatcgtg acaaacgggc tcgcagtatt
    30481 ggtgtacatt aaagaagata gcattttctc cgccgcctcc gacaacggtg cgggtccaaa
    30541 tggtattttt gccctgatgc agatacggcc gtatgtcgat gttcaggttt ttcctccagg
    30601 atttacctct ttcagcgtta cccaaccgag taccttcctc attacgtacc ggtgcaaacc
    30661 ttcttcctat aggaaatcca tcttcatctt ttgcagtata atctgccgca aacatctgat
    30721 tgttatagct gctgtacaca attttgccgt tgactttgac aatcagccaa tcatcgtact
    30781 caacccggct caaagtaaac agcgtcagtt tttccggatt tttcacattg agcgtcatct
    30841 cccgatcata ctgcccgtca cgccaatagt tgtcgccaat agtaccgaac atcagcctgt
    30901 gtgcgcttcc cgaagccgtc agcctgaccg tcatatcacc ctgcaggctg cctgcttcta
    30961 tacccgaacg ggtacagttt ggtcctgatt tctcgcatac cacctttaat ttcttgccgc
    31021 attgatactg ctggtttcga tatttgctct ccaggcagga atagttccag ccgggatcga
    31081 cattgacctt aagggccgcc tcgcacaaga acatctctgc gccatggtat tcattgcaac
    31141 gtttgacggt ttttaccgca ggaacggtaa tggtttccgt aggacatgcc gacgagggaa
    31201 ctgtgccgac gctgccgtct tcattgacgg gatatttcca tttgtatttt tccagtgttt
    31261 ctgccggatc gcctgcaatt ttctgcgtgc ctttaaccaa cttttcattt gaagatatgg
    31321 ttacatcggg ccgcttggta cggcttcctg caatcaggtt tacaccttcg cattcttgac
    31381 gaagatagag atccggattg gcaggcagat ttttacagtc gttgaatttg gcgtcaccaa
    31441 actgagtcgg aagagaaact ccgccaccgt aatactgttc agccattggt acgttgtctg
    31501 tatatcttgg ggtatattgg gcaggctgca tcatgtttgt tgcagtcccg gcccccaatt
    31561 ttcccagttc gccgacgtaa ctaccgtaat ctgcagcaaa ggccgtctgc atgaggccaa
    31621 atacagttag ataaatggca tgtcttttca ttttctgcct tccagaacgg ctaatttcgc
    31681 atttgcaata tcggaaagtt ttttatcttc agactcacgg atcaagtgtt ccaaagccga
    31741 atgcaaggac acatcaccct ttacggcaac atgttccgta cacatcccca attctgaatt
    31801 tacagcgcaa ccgtctaaac catcttcgct gtttcgggcg attaccgtaa cagggacttc
    31861 ggtgatgccg taatggtcga agagttcggg atgaatcagc atatcgcctt gcaaagccgc
    31921 aagttcttcg gccgccttaa ccgtggcttt cagtgagtcg tctttaaacc cgcggataac
    31981 cattaccccg cctgcctttg ctgtttcacg ggcaatgcgt ttcaagctgg cttcgggcat
    32041 tgaaaatgat acgaatacca ttaaatccgc tttttccgca gcctgtcggg cttcttgtga
    32101 ggcagtgcgg tatttttgtg cgatagacat cggatcagcc ttgatttttg catttccgta
    32161 atcacgaaca tcgggcacac ggtgcttgat acctttcaaa tccggaattt caggttgctg
    32221 ccgattgtca cccacatttg ggtttttaac tcgggtaact ttacccatgg ttgtacgcag
    32281 acggtttcgc gccgcctcta catccgcatc ggcgtaagca ctgcctactc cggccaaacc
    32341 gcccaaaaga atgaggaaga cgcctccggc tacgattaca ttgcgtaaag acttgtccat
    32401 cactccatct cctgtcaatt agtcttaaat ttatatgcac cgcagcagtc gcgcttacgg
    32461 aaaagctgga aagcaaactg attacctttg tatggaaatg ctttgccgct gtctctgact
    32521 aatgttgaac gtccaatagg ctcacagcaa cggcctctga attttcgggc ggccaccgga
    32581 taggtcatag ccattttata gttctgttta tccattacgg gattaagttt gtacccgcat
    32641 aaagcatccc tgcctgatgt cctccaagct actaagtccc tgtgcatttt tgtctgaatt
    32701 ctagccgcca tcaatttagc ggcctgcact tcggagaagt ggccggacat ccatcctgac
    32761 aaagggtaaa tcgttccgtg gcaacctgca caccaataca atttattgaa tggaaagccg
    32821 gcggccgctt taacacagtc agccgcacag gctgcttgtg caacgacatt ggcaaagagc
    32881 gcagaatcag gggcttgata aacggccaat gacggatatt tccaagtggg atcaagttcc
    32941 gtcatccaac cgatatccaa agtacctttt tccatacaat cgttgtccaa taaggcttcc
    33001 aaccaaaata cgaccggatt tttataccaa tgaacttgat aaaaggcatt atgctgatca
    33061 tgttgttcgt tacgaccacg cttatgcgag ccgccaaaaa gattttgaat cttattggat
    33121 acgtctgctt tacctgttgt ttccgccgta tctgcttttt gatttttaag tgaaataccg
    33181 cctaggctaa ccatacagaa gggggtgcgt acgacttcta ccaagtgtgt gggttcccaa
    33241 aatgataccg tcacgcctga acggctcggg tgcgtacacg aacaaaacca attgtttgga
    33301 gaagagccgt tatcctcttg attatccata ttcagattga ttttgccgcc gattttgatt
    33361 gggaacatac agctccaaca aatatcattt atcggattaa gcatcttccc ctggcaggtt
    33421 aatttattcg gtatcggagc agtaggaaca gcttcagcgg cgtgagatat agaaaataat
    33481 gggagtagta ataacggaat cagtaatttc agcggtgtga caaatatttt cacggtttca
    33541 tgacctctac acggagccgg cccccttctt gggagaccaa acttggaact tcattcagct
    33601 gaaaactttt ggacatccgc ccttcttggt cgtaataaac cgcctgatcc atttctactg
    33661 ataactcata ccaattgcct gcagtcagaa taggcatgaa tgattcgcca tattctgctg
    33721 caagtttctt ggccagttcg atttggtcag gttcccggcc gtcaaagaaa aacattttga
    33781 atgggattgg ggctgcttcc aaaggtttga caacagtacc ggcaggagca ataatttgac
    33841 cgtcttggtc ataaattgtt tcggttaagg tataacttgg gtcgaagtat cgaacagagc
    33901 ctttagttgc cttcgccagt ccttccaagg gctgtagatt taaggcggag cgatttattc
    33961 tatcttggac ctctttctgc attgccgcca attggccgct gtcttgttta gctttcaaac
    34021 gcgcttcaat ttcgcggatt gcatgttttt ccgctattgg gtatgtggtg ccgattttgt
    34081 cgccaacctt ctcgacagag ggtgtcgaag cggctaccca tgaggaaatt ccggttgcaa
    34141 gtattactag cgatgcgact gaagctattt ttttcttgtg ggacatactc actcctaaaa
    34201 aatggggacc gccttaccaa taatttgccc tgaatggatt aagccggata attcatatcg
    34261 actgtccaag ctgtctttgt gccctgtttt aacccacatg aaccgttgtg gaatttcccc
    34321 ttcgaatggg ttgatggcca gcttcatgcc atccagggag tattcttttg cagtacctac
    34381 ctctttccca tctatgaaaa aagtacgatt cgccttcgtt acatgatttc cgggaagtcc
    34441 ggcaacctct ttgacgacct gcgtaccaat agggtaaaaa ccaccttgcc atgcaaaggc
    34501 cacataatcg ccctgcttca gatcacttag cttgttcgca tcttttttga tgaagtaaag
    34561 atggtgaggc aaggaacggg aaagattaaa tgccaaagca taatgttgat tgaatgccga
    34621 caagcagacc gcaactgcta aaaaaggggt tgcatatttc tgacaaaact ccctataaag
    34681 acgtttcatc actgctttgt tgctttcttt ttgtgatatt tttatcattg cctcaaacat
    34741 taacgatcca tttcttcttc agtaaccggg gtgcctttac cctcttcggc catcggtttc
    34801 ggaattgtct tttcaacttg ttgatgtacc ggtgccgcca ctacagctgt ggccgggaca
    34861 cgcttatcca actctttcat aatgtcagca gtaacatcaa ccacctctgt gttccgtccg
    34921 accaatgcag atttgacaaa gatgttacag ccgcaccgaa ttaccttttc ttcgataacc
    34981 tgttgcaagg tattcatctt tgcttggtag ttttggagga tcgcggctcg ttgccggtcg
    35041 gaaattccat tctgcagtcc ttgattcttc gcctgttccc ggtaagtcat ggagataccg
    35101 gccaaatcta caatatggac cggtttctca tgggttttca tgaaattcca tacgcctaat
    35161 gccaatgcgg caatagaaat tgcgccgatc acaaggctgc ccaatgaaac ctctacgccg
    35221 ttatcctccg gattcttttc cggatcggta tttgccgtgt tttctttctt ttcttttgct
    35281 tcagtcatct tattaatatc ctatcctctg cctaattagc gggttgcggc acggtccctt
    35341 aaaactgctt caatggcctc ggctgtattc aacccctgat ttctgtaatg gttcacggct
    35401 tcaacgtctt ccgcttttga gcttgaggca agaacgttga atgggtccga gtacaaccta
    35461 cctactccat aacctatcgg ggtagagaca aatatttcac tgtagtagtc agttttgctc
    35521 aaagttcgca gaatctgcat aatcccgtca ttgacttcaa aaggttcttt gtcttgaatg
    35581 gttttgagcg atttagcccc ggtacgcaat tggaaaacaa agtcggcatt ctctaatgcc
    35641 gcctcagccg ccttagtctt gtagtagtca tttgcacctt gcgtcagagt gccgcatgca
    35701 cctttgtact tacggacacg gcggtagcct tcctcaataa aacgagcggc tgtacccgtg
    35761 tcgcccaaca aagcccaagc ctcgtcaatc atcataattt tgtactggct tcgtgatagg
    35821 tacatgccct gcataatgcg gtacataatc agctgcatga tgatctgttg cagatccggt
    35881 ttggttttta actcttcaag ttccagtacg accaggtcat ttttaaactc gacattcaat
    35941 tttccattga agtaactgcc gtagatacct tccgaacagt atgtgtacaa ttggtcggca
    36001 acggccacca tagacaaatc tcgttcaccg ggctttcctc cttcattact gccggtaatc
    36061 aaacacttga gacagcgttc ccttacatga tcgaccgtgc cttcttgtcc gaattcgtac
    36121 catacttctt cgattatccg ttgaagcttg gcttcctgct gttccgttag ggagtctgac
    36181 ggagaggcca tcaatgagaa gattagtttc aacatctcaa tatcttcgtt gatgtccgtt
    36241 accatttgga atggcgggaa gcacaggcct gcttcatagg taaattcaat atactgcccg
    36301 ccaatcattt ggcagaattt ttcatacgaa cggccaacgt cgataatcca tacctgagtc
    36361 cccagcccca tgtagttgcg cacaatttca ttggcaaaaa atgatttgcc ggaaccggaa
    36421 gcgccaataa tggcaaagtt atagttacct gccgggttgg caaataagtc gattcccatg
    36481 gcctgcccgc gttttccgaa caaaccgatg acaggtcttc ctaaaccgtg ccactcgccg
    36541 ataacgggag acatacagac agcattagtc atggttttgg ttgaccatct tttcttactg
    36601 tccaaatatt tctcaacctc tttgtcgata gccattggga gggcagtcaa atatgacccc
    36661 agttgaagat attggtcacg ataaagaccg aagccctgag cctgccaaat cgataccgcc
    36721 tggctttcag ccaagttaat gttttcggga cgctcccaaa tgacaacttg gtgcaacaaa
    36781 ctcaccagac cgccgccgcc tttgccgaac gcttctaggc atatgtcgta atcacgtttg
    36841 atttttgctg cctccggcat aactgcggcc atcttggatt ccgccgtttg tttggcgcgg
    36901 gccgctttca gttttacggt attgctggaa gtgtcataat cacgcttgaa catcgccatg
    36961 gaaatcagaa atggtgatgt gtaattcatg tttgcctcaa tgacatcacc aatcaatgcg
    37021 cccatattgg acagatgaaa ctcttcaggg tagcgattgg cagaatagca ttgagcaatc
    37081 acgcaatccc ccgcggcacg gttgccaaaa cgcagccccg agccgctatc aagaaccttg
    37141 atagatgtgc gggtctcaat catttgttca ctcaagaact tggtttcatc ccaaacagga
    37201 tcgatttcat cttgttgtcc ggtaaacatc ctattagggt tgagtaggag cgttgtccaa
    37261 gccaataaat cgtctgcatt ccaagttcgg ccgtaaatct gcatggagcg gagtgaatcg
    37321 cgcatgcggg caaggagcga aaccgccttg ctgacttcca ctgcattatc aggtggaata
    37381 ttcaaattta cgctgaggat acagcgcaga tttcgtaaac gaatggccgt attttctacc
    37441 aagaccttct gtgtaccctt attccaaaat tcagtacgtt ttttggccaa agtttcgaaa
    37501 gtgcttcgca tcggatcgtc tataggtcgt gatgcttgta cttgctcata ttccttgaag
    37561 taatttctta aatcggggga gccgaaaatt tgaacctgta tcggcgtctt ttcaggaagc
    37621 agcgcataca ggctgcccca cttaccgatc aggtcgtcac tgccgcctaa aaatgggtta
    37681 agttccagaa taaagcccat gcccaacggt ttgtcgcctt taacggacat cagcgtaaac
    37741 aactctgttt tggtatcgta tgcaccgtaa ttgagatagt cgctcaaatg gtcaaagtca
    37801 ggatcgacag ggttgttgtg caaggcttcc atcggttgat cgacatcatc cgaaccggcc
    37861 aaccaatttt taaattttga caggacgccc attacatacc tctatcgaac ggtaccggga
    37921 atacccgatg tttcctgcag atgaagattc acgccaacat cggggatgcg gtcatttgtg
    37981 ttgtttgaat tggttggaga agtctcaacc gaactgcctg aagttttggc cccatccccc
    38041 aataagcgga tgggacgata ttcatttata atctgttgcc cgttgtgggc aattaaccaa
    38101 tcaccctgat tcagaacgac atatgtgaaa ctttggtcat ttaaatcgcc gtccgtatca
    38161 agatagggag cgacccaaat gcgagcaatt tcactttgcg agcgaacggg cgtaccggag
    38221 gaataagctt gcgttaacat taatgtgcga acactttgat cagtgtaagg ttgtccaacc
    38281 tgtcttttca taccgtcttt taaaacacct gcaacagatg ctttatagtt actgcttata
    38341 gattcacaat aaggatcgtc actgttccgg gcattagggc atcggaattt ttcactaccg
    38401 ccgataccgg acatggttaa ggttgaacat ccgctaagaa tcaatattgc cgcagtaatc
    38461 gataaggctt tcaaacgcat cattctgctc ccgctccatt taaccatttt tccaattcgg
    38521 cacgcggcat ggcaccggat gtacgccgac catctttatg aatcatgctt ggagtaccgc
    38581 gaactttaag ctgctctgcg agtgcaatat tttctgatac agggttttta cagttaccgg
    38641 ccgccggttc tttatccatc agcatgtagt tattccatgc tttttcacga tctttactgc
    38701 accagatatg ttctgctttt gaaattgcat cagggtggag ggatttaatc gggaacatga
    38761 aaacgtatgc ggtatagtct gtcatgccgg ccaatgtttc ttccaaacga cgacagaacg
    38821 ggcaatccgg gtcggaaaaa agtgcaaatt ctcgcttgcc tttgccgcca ttacctttaa
    38881 cgattttgat ggcgtttttg aatggcagtc gggaataact cttttgtgtt accgctgaac
    38941 gggccatctc ggtcaagtct ttctgctctt gcatatcgta caacgcgcca aaaatgaaat
    39001 agcgaccgtt ttcttgtaca taggcaatgt tgtcacccat tgtcacttca taaattgaag
    39061 ccatgggggt agcctttact gaagaaaacg tggtattcgg atacaacttg tgcagagtgc
    39121 ttccaacagt tttgacatcg gcaaaggccg tctgaaaagc tgtgattgct gcaatagtgg
    39181 ccaagatttt ctttttcata gatggatcct atttggtttc gccggtatca atttcgatac
    39241 cttgagtcag tacgacatcg acttgacggc ctgcattgac ttcaactacc gggaagacct
    39301 tatcggccag ttttatgtaa tactcagcaa gcctgtcagc tgcaccaccg ataccagatg
    39361 caatccctgc acgatattga tcactgcccc gaatagaggt tgtgctgcca aatgcggtat
    39421 tattcgtcac cttgaatgct tccgatacgc ccttacccat accgctaatc acgccggaca
    39481 tcagtgcatt tgccaagagt tggccttgtt tcgttacaag attacctctc acgcctgttt
    39541 tgccgtcttc accggcaacg taacctttta ccggcatgtc gatggctcgg ccatcggtac
    39601 ctacacagct gagattctgc agtctgatgt aagcgcgttc tgaagatata tcgccgtaag
    39661 cctgcccggt tacgaaacat tctctgaaat tgtatttaaa ccggttggga agttgcgcaa
    39721 aatccgttac gcgcattaaa acagggtggg ggctggcatt ttgtgcctca cccccggtcg
    39781 gtgcgtcaac cccacctaac agtacggatc ggaaaaaagt accgctaggt atatacgagt
    39841 cacgcgcccg aaacttgcct tgcgagacga caaccttacc gccgtcttca actacttcta
    39901 tattcatacg cggggttttt gatttaaccc actccgcttc ttttaattgc ggggaaaccc
    39961 gtacattttc attcggattt ccggtattta ccgatccggt cgttccaggt tgcagctctg
    40021 cttcactcat acttcctgaa acgattttcc gttcaatggg cctatctgcc ggaggcgccc
    40081 cgttcggcaa tgtgcctgcc ggaggggccg gaggaagccg gccgttatcc ccgataccgc
    40141 taattgcagg cggtttgcct aatccatcag gccccagccc cttacttgta tcaggattac
    40201 tgctgttctc tttttctttt aactcctgaa ccatggtttc caattcgctg atttttcgat
    40261 ttgcatattc aatcttcgaa ctttctgtgt tcatccataa ggaattgtcc gtaatgcctg
    40321 cttgggggct tgtaaaatca gttctgagaa tctgccgttt gttttgaacc tgtgtgactt
    40381 cggcagattt ctttttattg ccttcaatga cataaattgc actgcccaca atcgccgcca
    40441 tgctgcccgc aaccatgccc gcgatggcta actggcgttt tcgcgctgct gaaagtttaa
    40501 atttgtttac tttcaccctc atctcaacct cccggacggc tgataataaa tacgtcggta
    40561 atttccccgg gttgtaggat ctgtttccga accgctaccg ccaaaactcc ttgtcggtaa
    40621 aattcctgct ctctaatttc aagttgttta gtacctaaat tagttaatgt aaaagaaagg
    40681 ccttgcatat cagcggcggt gtaacttccg ttttgaataa agagggtgtt tttccataat
    40741 gggacggttt gatattcatg actatgattg atgcccatat cgccggtagt gccacgcatc
    40801 atagaggcca tcattttttt gatggcccgt acatattctg tactattcat cgttacaggc
    40861 attggaccgg tttgcgaagc caatactgct gcttcacggc gattagctcc agccacatcg
    40921 attacgatgt tgtcaccctg ttttgatgta ggtttaagaa ccagcaaata ggtcttacct
    40981 tcttcgctta taacgaaaag atttgtcgga ttggaggtca aactacggac aaacagttgg
    41041 cctgttgttg tatctttctg aagctcaaga tcaccctcca taaatttcca tgatgaaatt
    41101 cgaccgcctt caatggcgat tctgctcaag ttccgtttac ttattgaaac aacgtgaggt
    41161 gtattttctg ttgccggcac tcgttgcgct gcaaaactag tcgcgggtcc ggctgccaac
    41221 aaggcagaaa agacaatttt ttttaacagt gaacacatat caattacctc gattaccgcc
    41281 tttaaattct gttgccgttt tactgatttc tacatcctcg gtttcaactt cggatttttc
    41341 ctgacctgcg ggttctgctt tagttacacg cttttgaact tctttaatcg ataataattg
    41401 cagtgttcca taattatttt tgaaagaaat aagtagctcg cggtcttttt gctctaccag
    41461 ggcattgttg cgtttaacct caaaaacccc acgaatggaa actgtattgt ctgaaatgtc
    41521 cgccgttgct tctgtcgcgc taaaagtggt gccgacatta tttttcttaa cccataaggc
    41581 atttaaagac agctccctct ctagggcatc tttataatca ggtgaaacaa tcttcaataa
    41641 ttgactattc tgataatcga ctgttttagg ggttacattt aagagaagat aggcagcata
    41701 gggagccatt tcttcaagat attctttaga aaaagcgatg tttgatacag tcattgaccg
    41761 ccggatttcc ggcggggtca gcgtcgtttg aacaatcgta cctttgaaga gcagggtcgc
    41821 ggacaaaaat agattgacag ctatagacat tcccaaaagt atccaaaaaa agacctttat
    41881 gccggttctt atcgccaatt cactcaacat atcttcagtc ttcaatttag aaactcccgt
    41941 ttattcgttt caggtatccg tttgtttttt tcattaatgg gtaagtacca gtaaagaaca
    42001 tgcaaaaaaa accattgatg cttgccagac ttaagcttgc cccacctcca tgcaatgaaa
    42061 aggctaattg acatggaaat gaaaaagtga cgagagacaa tacctatacc gaagccggac
    42121 ataaataata taaaaacgtc caagtcccaa ataaatgcct ttttgggatc gtctatcgat
    42181 accggcacat gataatctct catcacttct cttatctagt ttgattaaat aatcaaggta
    42241 aacatacctt taatgatgcc cgggcctaat gttgcggcca agccaacacc caaaccgacg
    42301 atacatggca tgatggtttg tttcatcagg cctaccgcca aaccgatcaa gaatgcagtc
    42361 aagcccaaag ccatacccag ataaccttca gaccagcctg ttaccatgtc agccaagcct
    42421 ttgaattcgg cacctgttgt agcggccatt gccaagttag catacaatgc caatgcaacc
    42481 gcactagcgg actttactaa tttattgcgt ttgatattca ttatgaacct cattaaacag
    42541 agcttatatc gccaagcttt tgcgtccgat cattctttac caagtgatta caacggttct
    42601 cgtatcatca ctattgcagc aggccgggga acttgctgtt cttatttttg cggatacatt
    42661 atgtttcctc agccatttac ctattactgc tgcccgttgt ttagccaaac gtctattaat
    42721 tgcagtattt cgacctccga tactgtcggc ccatccgcgt aaataaaccg tctgagtatt
    42781 tttcaattta ggcagaacac gtttgagcgt tctcacgccc tccgcaccga ggtgcgaact
    42841 gcctaaattg aaaaacaccc tgatctcatc tttttgaaga ttggtttgtg tttgagcata
    42901 aaccggttgt tccgccaaaa ccttgcgctc aaatcccgaa cactcttcat tgcggcagaa
    42961 gacaaattcg cccagctgat tttgttccac tgtataagtt gcgtcttgag gcagcccggc
    43021 cacttcctgt tttggggcgg cacaggctgt cagaatcatt gcggaggcta cgaagagcgt
    43081 gtttttaatc gtttccaatt ttgatatacc ttatttgcgt aagtcattct tgcgcgatag
    43141 cacgcccggc ctttaagttt ggtacatgcg gcgttataag caccaactgc ttcccatgat
    43201 ttcccatgtt gccgataact atttgccaat atccatgcac caatatgtac atttgtgcat
    43261 ggattccata catccgcccc tgttaaacca aatttcttgt ggagtacggg tagccatgaa
    43321 cggttgatac ccatcaagcc aatatcagct gtatgacttt caatattcgg cctgaaagcg
    43381 ctctcggtta atgcgatcga ttgcaacatg gcgggatcga tgccatattt gctgccggct
    43441 tccttataac aaaaagcaac tgctcttgca gatgtgagta ccaaaacacc cggtacgacg
    43501 aactgtaaaa gtatggccct taatctcatg tctttactgt cgttgtatgt atgactaatt
    43561 tttatctcga ttcgacaatt tttgttaaat caaacgaaat taactaaatt ttttagaaat
    43621 gtttc
//
LOCUS       DAWTHD010000003        40944 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWTHD010000003 DAWTHD010000000
VERSION     DAWTHD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40944
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.003"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(1..311)
                     /locus_tag="WM704_000097"
                     /pseudo
     CDS             complement(1..311)
                     /locus_tag="WM704_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357374.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(478..1329)
                     /gene="dinD"
                     /locus_tag="WM704_000098"
     CDS             complement(478..1329)
                     /gene="dinD"
                     /locus_tag="WM704_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFA9339623.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLCRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(1445..2989)
                     /locus_tag="WM704_000099"
     CDS             complement(1445..2989)
                     /locus_tag="WM704_000099"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFA9339624.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3064..4068)
                     /gene="rfaD"
                     /locus_tag="WM704_000100"
     CDS             complement(3064..4068)
                     /gene="rfaD"
                     /locus_tag="WM704_000100"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFA9339625.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAAAVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(4132..5094)
                     /gene="hldA"
                     /locus_tag="WM704_000101"
     CDS             complement(4132..5094)
                     /gene="hldA"
                     /locus_tag="WM704_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFA9339626.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDVIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(5150..5890)
                     /gene="pyrF"
                     /locus_tag="WM704_000102"
     CDS             complement(5150..5890)
                     /gene="pyrF"
                     /locus_tag="WM704_000102"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFA9339627.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            complement(6414..7061)
                     /gene="adk"
                     /locus_tag="WM704_000103"
     CDS             complement(6414..7061)
                     /gene="adk"
                     /locus_tag="WM704_000103"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFA9339628.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            7276..8115
                     /gene="htpX"
                     /locus_tag="WM704_000104"
     CDS             7276..8115
                     /gene="htpX"
                     /locus_tag="WM704_000104"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFA9339629.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(8351..9649)
                     /locus_tag="WM704_000105"
     CDS             complement(8351..9649)
                     /locus_tag="WM704_000105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFA9339630.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(9754..10905)
                     /locus_tag="WM704_000106"
     CDS             complement(9754..10905)
                     /locus_tag="WM704_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFA9339631.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            11240..12619
                     /gene="norM"
                     /locus_tag="WM704_000107"
     CDS             11240..12619
                     /gene="norM"
                     /locus_tag="WM704_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFA9339632.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHEAV"
     gene            12663..13703
                     /gene="murB"
                     /locus_tag="WM704_000108"
     CDS             12663..13703
                     /gene="murB"
                     /locus_tag="WM704_000108"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFA9339633.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            13715..14365
                     /locus_tag="WM704_000109"
     CDS             13715..14365
                     /locus_tag="WM704_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFA9339634.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            14420..15196
                     /locus_tag="WM704_000110"
     CDS             14420..15196
                     /locus_tag="WM704_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9339635.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            15246..15812
                     /locus_tag="WM704_000111"
     CDS             15246..15812
                     /locus_tag="WM704_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFA9339636.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            15832..16722
                     /locus_tag="WM704_000112"
     CDS             15832..16722
                     /locus_tag="WM704_000112"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFA9339637.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            16747..17505
                     /locus_tag="WM704_000113"
     CDS             16747..17505
                     /locus_tag="WM704_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HFA9339638.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(17560..18225)
                     /locus_tag="WM704_000114"
     CDS             complement(17560..18225)
                     /locus_tag="WM704_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFA9339639.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWRFVVVQNPEIRQAIKLFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIVTKARRPLEETV
                     IWA"
     gene            complement(18422..19330)
                     /locus_tag="WM704_000115"
     CDS             complement(18422..19330)
                     /locus_tag="WM704_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HFA9339640.1"
                     /translation="MQPPKASDIISHLQAGGRQRFFRRHLPAIQYTHLNNYLNIKDKQ
                     RMNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVARLTMTVYLPAEQKG
                     THMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIR
                     SLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTSDA
                     EVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKR
                     IKSFVVESENFESIHNHSAYAYIAYP"
     gene            19398..20555
                     /locus_tag="WM704_000116"
     CDS             19398..20555
                     /locus_tag="WM704_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HFA9339641.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(20619..21620)
                     /gene="hemB"
                     /locus_tag="WM704_000117"
     CDS             complement(20619..21620)
                     /gene="hemB"
                     /locus_tag="WM704_000117"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFA9339642.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(21804..22088)
                     /gene="yhbY"
                     /locus_tag="WM704_000118"
     CDS             complement(21804..22088)
                     /gene="yhbY"
                     /locus_tag="WM704_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFA9339643.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            22198..22818
                     /locus_tag="WM704_000119"
     CDS             22198..22818
                     /locus_tag="WM704_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFA9339644.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGTVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            22882..24849
                     /gene="ftsH"
                     /locus_tag="WM704_000120"
     CDS             22882..24849
                     /gene="ftsH"
                     /locus_tag="WM704_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFA9339645.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIQTLLNKNVRVKVTPEEK
                     PSALTALFYSLLPVLLLIGAWFYFMRMQAGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            25071..25502
                     /locus_tag="WM704_000121"
     CDS             25071..25502
                     /locus_tag="WM704_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFA9339646.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            25495..25773
                     /locus_tag="WM704_000122"
     CDS             25495..25773
                     /locus_tag="WM704_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFA9339647.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            25826..26404
                     /gene="pth"
                     /locus_tag="WM704_000123"
     CDS             25826..26404
                     /gene="pth"
                     /locus_tag="WM704_000123"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFA9339648.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            26465..26671
                     /locus_tag="WM704_000124"
     CDS             26465..26671
                     /locus_tag="WM704_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFA9339649.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            26668..27030
                     /locus_tag="WM704_000125"
     CDS             26668..27030
                     /locus_tag="WM704_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFA9339650.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            27259..28674
                     /locus_tag="WM704_000126"
     CDS             27259..28674
                     /locus_tag="WM704_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HFA9339651.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITITALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            28794..29303
                     /locus_tag="WM704_000127"
     CDS             28794..29303
                     /locus_tag="WM704_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9339652.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            29471..30853
                     /locus_tag="WM704_000128"
     CDS             29471..30853
                     /locus_tag="WM704_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFA9339653.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIAIGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(30909..31664)
                     /locus_tag="WM704_000129"
     CDS             complement(30909..31664)
                     /locus_tag="WM704_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9339654.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(31674..32390)
                     /locus_tag="WM704_000130"
     CDS             complement(31674..32390)
                     /locus_tag="WM704_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9339655.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(32380..33207)
                     /locus_tag="WM704_000131"
     CDS             complement(32380..33207)
                     /locus_tag="WM704_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9339656.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            33492..34400
                     /locus_tag="WM704_000132"
     CDS             33492..34400
                     /locus_tag="WM704_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFA9339657.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            34477..38079
                     /gene="recB"
                     /locus_tag="WM704_000133"
     CDS             34477..38079
                     /gene="recB"
                     /locus_tag="WM704_000133"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFA9339658.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIAHALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRQTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            38118..38477
                     /locus_tag="WM704_000134"
     CDS             38118..38477
                     /locus_tag="WM704_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFA9339659.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            38602..39081
                     /locus_tag="WM704_000135"
     CDS             38602..39081
                     /locus_tag="WM704_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFA9339660.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(39356..40735)
                     /gene="radA"
                     /locus_tag="WM704_000136"
     CDS             complement(39356..40735)
                     /gene="radA"
                     /locus_tag="WM704_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFA9339661.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADASTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT         9461 a        11837 c        10490 g         9156 t
ORIGIN      
        1 accacatttc atttttatgc gaaaatggtt tatcgtaata ttcgaattca ataatacctc
       61 ttgatttaac gataatagaa ggctctctat ggatatcctt atcaggaaca tcatcaaagt
      121 caactaaatg caaaagtttt tcctcctgca aaaatcttta acggcgcatt atctttatgc
      181 atttctttca tttgtccagc agttatctta gttccttttg tacgaactaa aacctccccc
      241 aatggcttcc actccaccgg tgccgtctga atcatctcaa tcaatttttt cgctttgctt
      301 tgcatatcca tggttgtttt ccgtttcctt cccgctgcgg cgggatatgg ggcggttgcg
      361 gggcgggttt cagcccgcta tgtccgtttg ccgttatttc tgcgggctga agcccgtcct
      421 gctttcaggc tgcctgaaat cgtgtgcagg ctgctttttc aggtagcctg aatgcggtta
      481 ttttttgccg ttttgatgct gttcggttgc ggcaagcctt ttctgttctt cgttctcaag
      541 ctgtttgatg cttttctcgg gtacgggctg gttttcaggc atgatgccgc ccagttcttc
      601 aatggtttgg cgcacttttt gtccgacttc gaaatgcacc cggtttgcct gtgttttgcc
      661 ttggatgttt ttgcggcgta gtttttcttc ggtttgcgtg gcacgaaaca gattggcagc
      721 cggttcgctg gcgttcatgt ggtctaggat tctctggctt ttcttcaagc ctttgcggct
      781 gtgtatgccc tgcttgtcca gtcctccgta tagcccccga tagccgtggt tttggaagac
      841 ggcgtactca acgggttttt ctacgcctgc atccttggct gcggcggcaa ggtcggtatt
      901 gtgttcgcgc agctgcctgc aaagcagcag gcgttgtttg tcttcgccca aactcctgaa
      961 tgctgcctcg tcttgcagct cttggcggcg ggcttgcacg gcaaagtagg tttgccctgc
     1021 ggcaatgacg cttttggacg gatcgccgtt ttgcacaatc aggtagcagg catagcggga
     1081 aaggcggtaa tccgccactt cgcgctgccc gcctttccca agggctatca ttttgttggt
     1141 ttcaacaaaa tgatgatttt tgtcatttcc tgatgtttcg caagcggtta tggctttatc
     1201 aatggcccgt tggaaattgc gccattcgga atattccaag atttgctgca aggtgcgcgc
     1261 cgaccaatat tcattgcctg tttcgtcgat gtgtttggcg ttttcaaaag cgttattttc
     1321 tgttgtcatg tattttcccc gaaaatttct tgatttgaac ttgtaaggat tgcttacagg
     1381 ttgccgcccg tattttcaga cggcctcttt cccattcagt tgttgcaaaa tatgcaacaa
     1441 ctgcctaggc ctcaatctct gcaatcactt cgtcaatttc acgccgcagc cgttcgattt
     1501 tggcgacggt ttcgctgatt tcggcgttaa gctgtctgat gtcgatgacc tcgcgggtgt
     1561 cttcggcttc gacatagctg ctgacggcga ggttgtagcc gttgtctttg acggtttgct
     1621 gggcggcgtt ttgggcgata tgcggcacat cggctttgtc ggcgaagagt ttgacgattt
     1681 cggcaatgtg ttcttcggtt aagacgttgt tgttggtttc ttttttaaag aagccgcctg
     1741 catcgatgaa ttggatgtcg gtattgtctt tgtgtttgga caaaaccagg atattgacgg
     1801 cgatgcaggt gccgtaaaag agattgggcg caagggcaat cacggtttcc acatagttgc
     1861 cctccaccag atattggcgg attttctgct ctgcgccgcc gcgatagaaa atgccgggga
     1921 atgagacgat agcggcgcgg cctctgccgg aaaggtagtt cagtgcgtgc aggatgaagg
     1981 caaaatcggc tttggatttc ggtgcgagta cgcctgcggg ggcaaagcgg tcgtcgttga
     2041 tcaaggtggg gtcgtcgctg cctatccagt cgatggaata gggcggattg gagacgacgg
     2101 catcaaaggg tttgctgtct ttgagtttgg ggttggtcag cgtgtcgccc aattcgatgt
     2161 ggaatttgtt gtaattgacg ttgtgcagaa acatattcat gcgggcgagg ttgtaggtgg
     2221 tgtggttgat ttcctgcccg aagaagcctt cttcgatgat gtgttcgtca aactgttttt
     2281 tcgcctgcaa gagcaggctg cccgagccgc aggcggggtc gtagattttg ttgactttct
     2341 cctgcccgtg caccgccagc cgcgcaatca gcttggagac gctttgcggg gtgaaaaatt
     2401 cgccgccgga tttgcctgcg ttggcggcgt agttggaaat caggtattcg taggcatcac
     2461 cgaaaaggtc gatgcggtgg tcttcaaaat tgccgaaatc gagttccgcc acgcctttaa
     2521 ggacggcggc aaggcgtttg tttttgtcgg caacggtgct gccgagccgg ctgctggtgg
     2581 tgtcgaagtc gtcaaacaag cctttgatgc cttgttcgga cgggtagccg gaggcggagc
     2641 tttcaatcgc ggtaaagatt tctttcagct tggtgttgag ctcttcgttt tgatgggctt
     2701 cggcggcaat attgcaaaaa agctggccgg ggtagatgaa atagcctttg actttgacgg
     2761 catcgtcttt gatttcgggc gtgatgatgc tgtccggcat agcggcgtaa tcaatgctgc
     2821 tgtcgccggc ctgcatatag tcggtgaagt tttcgctgat aaagcggtag aaaagtgtgc
     2881 cgagaacgta ttgtttaaag tcccagccat ccaccgcgcc gcgtacttcg tcggcgattt
     2941 tccaaatttg gcggtgcagt tgggcgcgtt gttgcatttc ggtcatcatc gaaatccata
     3001 tataaagtta aacaaatcaa aatcgcctga tattttcaga cgattttttt acgggcattc
     3061 aaattaagcc aaattttcca gcatccattt gacgtagcgg ttgacacctg ctttgacatc
     3121 gaaaaattct tccttatatc cggcttcgcg caatttggtg atgtcggctt gggtgaagcc
     3181 ctggtatttg cctttgagcg cgtcgggaaa ggggatatag cggataagtt cttcttctac
     3241 caactctttc aagctcaatt cagatttgcc ttcggcggcg cggcaggcgt tgacggcggc
     3301 ggcggcgagt tcgttgaact gttggctgcg gccggtgccg aggttgtaga tgccggaaag
     3361 ttcgggatgg tcgaagaagt agaggttgat tttggcgacg tcttcgacgc tgacgaagtc
     3421 gcgggtttgt tcgccgttgc cgtagccgtc gttgctgccg aacaggttga cgtaaccgtg
     3481 ttcgcggtat tggtggaaat ggtggaaggc gacggatgcc atgcggcctt tgtgttgttc
     3541 gtgttgtccg taaacgttga agtagcggaa gccgacgact tgggcggtga gaccttcttt
     3601 catgcggcgg cgcaatactt ggtcaaacag gaatttggag tagccgtaca cattaagcgg
     3661 tttttcgagt tcgcgctctt cgcggaagat ttcgcctttg ccgtaaaccg ccgcactgga
     3721 ggcataaagg aaggggatgc gttcgtcctg acaccagtcc aacaaatcca gcgtgtactg
     3781 gtagttgttt tccatcatat acaaaccgtc gtggttcatc gtatcggaac acgcgccttg
     3841 atggaaaacg gcttcgatgt tttgataagg taaaatgtgt tccctcactt ggcggataaa
     3901 ttcgtgtttg tcgaggtagt gggcgatttc gcactcggca aggtttttga atttttcgcc
     3961 ttttgtcaga ttatcgacgg cgacgatgtc ggtaatgccg cgttggttga gggctttgac
     4021 gatgttgctg ccgataaagc cggccgcgcc tgttacgatg atggtcatat tcggtttcct
     4081 ttgttttgag ggtttcagac ggcattggat ggtcatgccg tctgaaaaga tttattgccc
     4141 gtccagtgct tcaaccaact ctgcaaacga gcaaaccgcc gtaccgagtt tcgccacgac
     4201 aaccccggcc gcagtattgg caaggtacat ggcttcgggc atggtgcagc ctgccgccag
     4261 ccccaagccc attccggcaa tgacggtgtc gcctgcgccg gacacgtcgt aaacttcttg
     4321 ggcgcgggtg ggctggtaaa tgggttcgcc ttcgctgaac agggtcatgc cctcttcgct
     4381 ccgggtcagc aaaacggcgg tcaagtcgag gtggcggcgc aggttttgcg ctttttcggt
     4441 cagatcgcct tcgtttttcc aactgccaac cacttctttc aattcggcgc agttgggcgt
     4501 aatcagagtt gcgccgacat atttttcgta atcgtcgcct ttggggtcga ttaagacggt
     4561 tttgccgaca tgtttcgccc aatcgatcat atcggagata tgcgacagac cgcctttgcc
     4621 gtagtccgaa aaaatgatta cgtcgtattc gggcaagatt tcacggtatt tccgcttgat
     4681 ttgttccaac acttcgcggt tgggatgttc ttcaaaatca aggcggataa gctgctggtt
     4741 gcgggcgacg acgcgcagtt tgacggtggt ggcgatttgt ttgtcgcgca tcagatagga
     4801 ggcgacgccg tcctgcacca tcagcgcgtc gagcgcggcg gcggcttcgt cgtcgccggt
     4861 tacggacaac agccctgccc tgccgcccaa cgaagcgatg ttgcgcgcaa cgttcgccgc
     4921 gccgcccgcc cgttggtcga ttcgtccgat ttttgccacc ggcacgggtg cttcgggcga
     4981 aattcgggac acgtcgccaa accaatagcg gtcgagcatc acgtcgccga caacaagaac
     5041 tttggcttgt gcgaaacggg atttgagggt ttcttgttgg aacttggcgg gcatattgcc
     5101 tccgttgtgt tttgttcaga caaccttaac ctgtaagacc gcctgaaaat cagtttgctt
     5161 caaggtttgc cacgcggttc acttcgcgca atacggcgac cggatcggcg gctcgggtaa
     5221 cggggcggcc catcaccaga taagtcgaac ccgccgccaa ggcttcggcg ggtgtcataa
     5281 tgcggcgttg gtcgtcgttg ttgccggcaa cgtccaagcg gatacccggt gtaaccaaga
     5341 caaaatcccg tcccaattcg cggcgcagcg gcgcggcttc ttgggcggaa cagaccacgc
     5401 cgtccaagcc cgaactttgt gccagttttg ccaaacggat gacttgttct tcaggggcgg
     5461 tgttcaaacc gatttccgcc aaatcgcttt gctccatgct ggtcaacacg gttacgccga
     5521 ttaagagcgg tttcgtgccg tatccggcaa cggcttcggc ggcggcttcc atcatacgtc
     5581 tgccgcccga cgcgtgcata tcgaccatcc acacgcccat atcggcggcg actttacacg
     5641 cctgagcgac ggtgtgggga atatcgtggt atttcaaatc aagaaagagt ttgaaaccct
     5701 gatgaatcag gctttccgcc aaactgcgtc ccgtcgcggt aaacagctct ttgccgattt
     5761 tgatttggca caacgccgga tcaaggttgc ggacgaatcc gagcgtgtct ttttcgttgg
     5821 caaaatcaag ggcaacgatg acgggggtac gttgttgcgg agtttggaag tcggtgatta
     5881 aaggattcat ggtgttttcg tttcgggttt tcagacgacc tttatcgtct ttttcgcagt
     5941 atagcggatt aacaaaaacc ggtacgtcgt tgccccgccc cggctcaaag ggaacggttc
     6001 cctaaggtgc ccaagcacca ggcgaaccgg ttccgtacta tccgtactgt ctgcggcccg
     6061 ccgccttgtc ctgatttttg ttaatccgct ataacaacgt aaattcaaac cgtaggccgg
     6121 gcattgatgt ccgacctacg cggactcaag cccccgacaa actgtttcgg ataaatttga
     6181 accggtacat cagccgtcat tcccgcgcgg gcgggaatcc attgataaaa ctcagtttat
     6241 cagatttaaa cagcgattgc ccaaattcag aaatggattc ccgcctgcgc gggaatgacg
     6301 ccgaatgatt atttttcggt tgcaacacgc tgtttatcaa aaacaggtcg tctgaaaacc
     6361 tgattttctg ttttcagagt gcgaagcctg cccgtgggta cgaccttttc gatttatttg
     6421 cccaatgcgc ccaatacttc ggctttcaca gcttctactg cttgggtacc gtcgactttg
     6481 atgtatttcg gcgcgtgttc gccttccagc ttgctgtaaa aatcaaccaa aacttcggtt
     6541 tgctcgtggt aaacggcaag acgttttttc acggtttctt ctttgtcgtc gtcgcgctga
     6601 atcaaatctt cgccggttac gtcgtctttg ccttcggttt taggcggatt gtaggtaacg
     6661 tggtaagtac ggccggaagc caaatgcacg cggcggccgc tcatgcggtc gacaatcacg
     6721 ctgtcggata cgtcgatttc aacgaccgcg tccaaaccca cgcctgcttc caccatcgct
     6781 tcggcttgcg ccaatgtacg cgggaaaccg tcgaacagga aaccgttttt gcagtcgtct
     6841 tgcgcgatgc gttctttgac catgccgata atgatgtcgt cgcgcaccaa accgccttca
     6901 tcgatgattt ttttcgcttc caaacccaag ggcgtgcctg ccttaatcgc ggcacggagc
     6961 atgtcgccgg tagagatttg cggaatgccg aacgcggcgg tgatgaattg cgcctgagtg
     7021 cctttgcccg cgcccggtgc gcctaaaagt aatgctttca tgaagtgatc cttttaaatg
     7081 tgtttattgg aatcaaacag atggtttacg attctacacg gcttggcgca atagttcaaa
     7141 aaccaactgc acaaactgcc atttgcaagc aaatgttttg catacctttg ccgagcctga
     7201 tattcacaag gcggcaactt tttgggacaa tccccaaagt ttcaaaacat cacaccctac
     7261 caaaaaggaa catccgtgaa acgcattttt ctgtttttgg ctaccaatat cgctgttttg
     7321 gtcgtaatca acattgtttt ggcggttctg ggcatcaaca gccggggcgg cgcgggcagc
     7381 ctgttggcgt attccgccgt cgtcggcttc actggttcga ttatttcgct gctgatgtcc
     7441 aaatttatcg ccaaacaatc ggtcggtgcg gaagtcatcg acacgccgcg caccgaagaa
     7501 gaagcctggc ttctgaacac tgtcgaagcc caagcgcggc aatggaacct gaaaacgcca
     7561 gaagtcgcca tctaccactc ccccgaaccc aatgcctttg ccacgggcgc atcgagaaac
     7621 agctccctga tcgccgtcag caccggtttg ctcgaccata tgacgcgcga cgaagtggaa
     7681 gccgtgttgg cgcacgaaat ggcgcacgtc ggcaacggcg acatggttac gctgacgctg
     7741 attcaaggcg tggtcaatac ctttgtcgtg ttcctgtcgc gcattattgc caacctgatt
     7801 gcccgaaaca acgacggcag ccagtcccag ggaacttatt tcctagtcag catggtattc
     7861 caaatcctgt tcggcttcct tgccagcctg attgtcatgt ggttcagccg ccaacgcgaa
     7921 taccgcgccg acgcgggcgc ggcaaaactg gtcggcgcac cgaaaatgat ttccgccctg
     7981 caaaggctta aaggcaaccc ggtcgatttg cccgaagaaa tgaacgcaat gggcatcgcc
     8041 ggagatacgc gcgactccct gctcagcacc cacccttcgc tggacaaccg aatcgcccgc
     8101 ctcaaatcgc tttaaaccga tttgaaacga caaaaaaccg tacgcacaag cagtacggtt
     8161 ttttcgtata tgccgactga aaacatcagg tttccatacc ggcaccaacc gtatccattt
     8221 ccgcaccgga tacgccgcat tgatatatcc ccgacaaacc gtgtccggac agaaaatatc
     8281 agataaaaca gcggtaaaaa tgccgtctga aaccaattag ggcttcagac ggcattttta
     8341 ctgcaaaacc ttatgcgaac ggatgatgca gcacaatcgt ttcttcgcgg tcggggccgg
     8401 tggagacgat ggcgaccggc gcgccgcaga cttcttcaat ccgtttcaaa tatgctttgg
     8461 cattggcagg caatgcgtcg tagcttttca cgccgacagt cgattcgcgc cagccgggca
     8521 tggtttcgta aatcggtttg caggtttcca ccgcatccga accgcaaggc aggatgtcgg
     8581 ttttgccgcc gcctggcaat tcgtagccga cgcagatatt gatggtttca acgccgtcca
     8641 ttacatcgag tttggtaatg cacataccgg aaatgccgtt gatttggatg gagcgtttca
     8701 gggcggcggc atcaaaccag ccgcagcggc gcgcgcggcc ggttaccgaa ccgaattcgt
     8761 gtccgcgctc cgccaaacct gcgcctactt cgtcgaacaa ttcggtcggg aacggacccg
     8821 aaccgacgcg cgtggtatag gctttgacga tgcccaaaac ataatccagc atttgaggac
     8881 ctacgcccgc gcctgccgaa gccgcgccgg cgagacagtt ggacgaggta acgaaggggt
     8941 aagtgccgta gtcgatgtcc aacaacgcac cttgcgcgcc ttcaaacagc agtttttcgc
     9001 cgtttttgtt tttttcgttc aacacgcggg acacgtcggc aatcatcggc gcaatgcgcg
     9061 gcgcgacttt ttcgataacc gccatcacgt cttccgcttt aaccggtccg gcgttgtgca
     9121 ggtattggag ttggacgttg taataggcaa ggacggcatc cagtttttca cgcagttttt
     9181 caggatgcag caaatcggcg gcgcgaatgg cgcggcgtgc cactttgtct tcgtaggcag
     9241 ggccgatgcc gcggccggtc gtgccgattt tgcctttgcc gcgcgatgct tcgcgggctt
     9301 ggtcgagcgc gatgtggtaa ggcaggatca gcgggcaggt cggcgcgatt ttcagacggc
     9361 cttcgacgtt tttcacgcct gccgcgttca actcgtcaat ttcgcccaac agggcttcgg
     9421 gggaaacgac aacgcccgaa ccgatgaagc aatccaatcc ttcgtgcagg ataccgctcg
     9481 gaatcaggcg caaaatggtt tttttgccgc cgacgaccaa ggtatggccc gcattgtggc
     9541 cgccttggaa gcgcacgacg ccgccggctt cttccgccag ccagtcaacg attttacctt
     9601 taccctcgtc gccccactgt gcgccgatta ctacaacatt tttagccata gccatataac
     9661 ctatcgatat taaaaattat tgcggaaaat ccccaaaccg gtgcggcttt gcgccccgcc
     9721 ccggtttgcc tttcatccgc tatgaacggg tatttagcgt ttcacgacct gccaaacgcc
     9781 gtccgtcttt ttcagacggc ctgcaagctc ttccgaaaca ttgtgtccga taccgtagtc
     9841 aatcacgaca cactgccctt gttcacgcaa ggcttcgacc gcttcgcgcg ccgcttctgc
     9901 atcttccgca tcgaccaaca cggcgggctg ccgttcgacg gcgggcaaac gcccgataaa
     9961 gctgcgtaag tcgaaactga atcccgttgc cgggcgcgcc ctgccgaaat atccgcccaa
    10021 tccgtcataa cgcccgccgc gcgcgaccgc gtcgtggaaa tcggcggcat aggcggcata
    10081 cagcaagccc gtgtggtaat tgtcgacgcg cagctcggac aagtcgatat ggatttcatt
    10141 atcggggaat gcgtcgcaca ccgcctgcaa ttcgtccaac gcgccgccga ccgccgacaa
    10201 atccggcaac cgtccgcgcg cgtcggacaa cacttcacgc ccgccgtaca ggcgcggcag
    10261 caacgagaat gcttttgccc acataccgtc cagcttccaa gccttgacct gcgcttcgac
    10321 cgacccggta tctttatcct gcatcaaggc aagcagcgtt gcggactgcc ccgcatccaa
    10381 atgcgccgca tcggacaagg cgcggaatat gccgatatgc cccagcgaaa gcagcacttt
    10441 gcccatatcg gcaattttca tgcttttcag cataaggtct atcagctcga tgtcgccacg
    10501 gatgtcggca aaaccgtaca tttccgcccc tgcctgcaag ggttcgcgca tattcggcag
    10561 accgtcgggc tgcgcgtgca acaccggacc ggcataacac aaccggttaa tcccttggtt
    10621 ggcggacaaa agatgggcat cgatacgcgc cacctgcggc gtgatgtcgg cgcgtatgcc
    10681 caactgcctg ccgctgagcc tgtccgttac caaaatggtt ttcagtgaaa gccccgcatc
    10741 gatatgcgtc agcagggaat gtgcgtactc catcagcgga ggctgtacca gttcataacc
    10801 gtgtacgcgg aacagtgcca acaactgctc gcgcgcgctt tcaagctgcc gcgcgttcgt
    10861 gggcagtacg tcggcgatat gttccggaag ctgccatgtc tgcatgagaa tctgccttct
    10921 gttttatcct gtaaacacaa aagactgccc gatccgcaat cagacggcat ctttgccgca
    10981 acgcatcacg ccgctgtcaa aacggcgatg cttccttccg attcagaatc cgaacagccc
    11041 ctgtccgacg gctcaatgcc gtcaagtcgt taaaaccaaa ctttaccata aaatacacac
    11101 aatctgaata tacttttata ccccgtatcc gccgtctgac ggcacggtat tttttactat
    11161 ataatgtgca ccattatttc tcatcacttc ccgctgccgt ttttttgttt cagacggcat
    11221 ttttattgac tgaacactta tgctgctcga cctcgaccgc ttttcctttt ccgtcttcct
    11281 gaaagaaatc cgcctgctga ccgcccttgc cctgcccatg ctgttggcgc aggtcgcgca
    11341 ggtgggcatc ggtttcgtcg ataccgtgat ggcgggcggt gcgggcaagg aagatttggc
    11401 ggcggtggct ttgggcagca gcgcgtttgc cacggtttat attaccttta tgggcattat
    11461 ggcggcgctg aacccgatga ttgcccagct ttacggcgcg ggtaaaaccg gtgaagcagg
    11521 cgaaacgggg cggcagggga tttggttcgg gctgattttg gggattttcg gcatgatttt
    11581 gatgtgggcg gcgattacgc cgttccgcaa ctggctgact ttgagcgatt atgtggaagg
    11641 cacaatggcg cagtatatgc tgttcaccag cttggcgatg ccggcggcaa tggtacaccg
    11701 cgcactgcac gcctacgctt ccagcctgaa ccgcccgcgc ctgattatgt tggtcagctt
    11761 tgcggcgttt gtgttgaacg tgccgctgaa ctatattttc gtttacggca aattcggtat
    11821 gcccgctttg ggtggcgcag gttgcggcgt ggcgacaatg gcggtgtttt ggttcagcgc
    11881 gctggcattg tggatttata tcgccaagga aaaattcttc cgcccgttcg gactgacagc
    11941 gaaattcggc aaaccggatt gggcggtgtt caaacagatt tggaaaatcg gcgcgcccat
    12001 cgggctgtct tattttttgg aagccagcgc gttttcgttt atcgtgtttt tgattgcgcc
    12061 tttcggcgag gattatgtgg cggcgcagca ggtcggcatc agtttgtcgg ggattctcta
    12121 tatgattccg caaagcgtcg gctcggcagg gacggtgcgc atcggctttt cgcttgggcg
    12181 gcgcgaattt tcgcgggcgc gttatatttc aggagtatcg ctggtgtcgg gctgggtgct
    12241 cgccgtgatt accgtgcttt ccttggtatt attccgttcg ccgctggcaa gcatgtacaa
    12301 cgatgatccg gcagttttaa gcattgcctc caccgtcctg ctgttcgccg gcctgttcca
    12361 accggcagac tttacccaat gtatcgcgtc ctatgccctg cgcggctaca aagtcaccaa
    12421 ggtgccgatg ttcatccacg ccgccgcctt ctggggctgc ggcctgctgc cgggctatct
    12481 gctcgcctac cgtttcgata tgggcattta cggcttctgg acggcattga ttgcctcgct
    12541 caccatcgca gccgtcgcct tggtgtggtg cttggaaaaa tacagtatgg agttggtcaa
    12601 atcacacgag gccgtctgaa aacgcagcac acttcaattc aaatacagtt aaagctcaaa
    12661 ccatgcaacc catccgatac cgaaccgacc tcacccccta caacactttc ggcctgcacg
    12721 cccaagcccg ggcctttatc gcgcttaaac acgccgacga attgcgcgac atcgtccgac
    12781 tgcccgagtt cgaccgcgat actgttttat ggctgggcgg cggcagcaac atccttttga
    12841 tgcaggatta cgacggcttg gtcgtccata tggaaaacaa gggcatacgc gagattgcgc
    12901 gctcagacgg catggttctg attgaagcgc aggcgggcga aatttggcac gatttcgtgt
    12961 tgcacaccgt cgcgctgggt ttgagcggtt tggaaaacct gagcctgatt ccgggtacgg
    13021 tcggcgcgtc gcccgtgcag aacatcggcg catacggcgt ggaggcgaaa gatgtgattc
    13081 acagcgtgcg ctgcttcgat ttggatacgg aaacctttgt aaccctttcc aatgccgact
    13141 gccgcttcgc ctaccgcgaa agcctgttca agcaggaagg caaagggcgt tatgtgattg
    13201 tttcggtcgt atttgcatta aaaacgcatt ttgtgccgaa cttgggctac ggcgatttgg
    13261 cggcgaaggt tgccgaattg agtgcaggta gggaggcgac ggcgaaagac gtttccgatg
    13321 cggtatccgc catccgcaac agcaaattac ccgacccgaa agttttgggc aatgtcggca
    13381 gtttctttaa aaaccccgtc gtcagcgcgg aaaaagccgc caccctgttg cagcggcatc
    13441 ccgatatgcc gcgctatccg cagcccgacg gttcggtcaa actcgccgcc ggctggctga
    13501 tcgaccaatg ccgtctgaag ggcttccaaa tcggcggtgc ggcggtacat gaccggcagg
    13561 ctctggtttt ggcgaacaaa aacaatgcct cttcagacga catctggaaa ctggcgcaac
    13621 acgtctgcaa tacagtattt actcgatttc aggtagaatt acacgccgaa cccaattggc
    13681 tgcccgcttc gttcagcctg taaatccaag gagtatgccc gtgacccgca ttgcaaaaac
    13741 caatacttac acccgcatca tcgacgccag ccttgcgctt ttcaacgagg aaggcgagcg
    13801 caacatcagc accaaccata ttgccgccca cttgggcatc agtccgggca atctctatta
    13861 ccacttccgc aacaaagacg aaatcatcgt ccaactgttc aaacgttaca gcgaagccct
    13921 gctggaatac ctcaacgaag ccgtgttgcc ctctgatgtg gaagactcta tcaactatat
    13981 ggcaggcatt tacgatgtca tgtgggaata ccgcttcctc tttagcgacg taaacaccct
    14041 acttgcgcgc agtgccgaat tgttgggcga acacaatacc tttacccaag ccaaagtctc
    14101 gccgcttcta gtcaaactgc tgacccagct caacgggctg aacgtcatca acgccgacca
    14161 aaccgctatg aacgacctcg ccgtcaatat gtggatggtt acgaaatact ggttcgactt
    14221 cgacagttcc ctgcgcggcc gtaccaagct gaccgaagac tccaaagcac gcggcgtccg
    14281 tcgtacctta agcctccttc gcccctatct tttgcccgaa caccgcgcgg aatacgaccg
    14341 caaaatcagc aacggcaacc cgtaaaccca taacgtccga atcagccttc agacggcact
    14401 tacccgacgg tatcagcgaa tgcgcgcagt ccccccgccc catatctcca tcacaggcct
    14461 aggttacctc ggcctgccgc tggcacaaaa gttttaccga cacggcagcc gcgtcgccgc
    14521 cataaaacgc agcctgactt cggacgacat caacctgccc atacacctcg acaccttcga
    14581 cctcaaccgt accgatacct ttcagacggc attgtggcgg catcacgccg acaaacccgt
    14641 ctggttcttc ctgctgccgc cctccttttt ggcgcattac gccgacaccg tcaaacaatg
    14701 ggcagagctt gcccgggcgt gcaacgtgca acacctgatt ttcacaagca gcacaagcgt
    14761 ttacggcgat aaagcgcgag aatgcaacga aaccgccgca cccgatccgc aaaccgagtc
    14821 cgcccgacaa atccttgccg ccgaacaaca cctgctcgac aacggcgttc cgaacatcga
    14881 catcctgcgg ctgggcgggc tttattgcgc cgaacgccat cccgtcagcc gcctcgtgca
    14941 aaagcaaaac atccaaggcg gcaaccggcc tgtcaatatc gtccaccgcg acatcgccgt
    15001 cgaaaacctg tttcagacgg catccaaccc cggcggcagg cggctgaaaa acattatcga
    15061 accgcgccac ccgacacgcc gcgaatttta tacggaagaa gccgccaaac tcggcctgcc
    15121 cgcgccggat ttctcacccg acgacagcgt gggcaaaatc atccgtaccg tttgcgataa
    15181 cggcttaagc ctgtaaaata agcggcaaca gcaacaacag gcattctccg ccccaatcag
    15241 aaaatatgtc cgccctcctc cccatcatca accgcctgat tctgcaaagc ccggacagcc
    15301 gctcggaact tacctccttt gcaggcaaaa cactgaccct gaacattgcc gggctgaaac
    15361 tggcgggacg catcacagaa gacggtttgc tctcggcggg aaacggcttt gcagacaccg
    15421 aaattacctt ccgcaacagc gcgatacgga aaatcctcca aggcggcgaa cccggggctg
    15481 gcgacatcag gctcgaaggc gacctcatcc tcggcatcgc ggtactgtcc ctgctcggca
    15541 gcctgcgttc ccgcgcatcg gacgaattgg cacggatttt cggcacgcag gcaggcatcg
    15601 gcagccgtgc caccgacatc ggacacggca tcaaacaaat cggcaggaac atcgccgaac
    15661 aaatcggcgg attttcccgc gaacccgagt ccgcaaacac cggcaacgaa gcccttgccg
    15721 actgcctcga cgaaataagc agactgcgcg acggcgtgga acgcctcaac gaacgcctcg
    15781 acaggctcga acgcgacatt tggatagact aaccttcaga cggcatccga catgaacagc
    15841 ccttttcaca acatcggtat cgtaacccgc cccaacacgc ccgacatcca agataccgca
    15901 cacacgctga ttaccttttt gaagcagcac ggctttaccg tctatctcga cgaagtcggc
    15961 gtaagggaat gctgcatcta tacccaagac accgacggct gccatatcgt caacaagacc
    16021 gaactggggc aatactgcga cctggtcgcc gtcttaggcg gagacggcac ctttctctct
    16081 gccgcccgcg aaattacccc ccgcgccgtt ccgattatcg gcatcaacca aggtcatttg
    16141 ggctttctta cccaaattcc ccgcgaatat atgacggaca agctattgcc cgttttagaa
    16201 ggaaaatacc ttgccgaaga gcgcatcctg attgaagccg cactgatccg cgaaggcaaa
    16261 accgccgaac gcgccctcgc cctcaacgat gccgtcctct cccgcggcgg tgccggacag
    16321 atgattgaat ttgaagtctt cgtcaatcag gaattcgtct atacacaacg ttcggacggg
    16381 ctgattgtct ccacccccac cggatcgacc gcctattcgc ttgccgccgg cggcccaatc
    16441 atgcaggcag gattacacgc cttcacgctc gtccccatct gcccgcaatc tatgactaac
    16501 cgccccatcg ccattccaga cacgtccgaa atcgaaatcc tcgttaccca aggcggcgac
    16561 gcgcgcgttc atttcgacgg acaatccttc atcgacgtgc aaaacctcga ccgcatcatc
    16621 atccgccgct accacaatcc cttacgcatc ctacacccga ccgactacca atatttcaaa
    16681 accctgcgcc aaaaactgca ctggggcgag caattagtct aagccggcct tcacccgaaa
    16741 gtacccatga attccaaaat ttcctccgac catacagaag ataccatacg cctctccaaa
    16801 cgtatggcac aactggggct ttgttcgcgc cgcgaagccg acggccatat cgaacagggc
    16861 tgggtaacgg tcaacggcaa aaccgccgta ctcggtcaga aagtttcacc ggcagaccgt
    16921 atcgaactga acaagaaagc ccacgaacag caggcggcac gcattaccat cctgctgaac
    16981 aaacccgtcg gctatgtcag cgcacaagcg gaaaaaggct ataaatccgc cgccgaactg
    17041 attacccctg aaaattgctg ggaaggcgac accagccgca tcagtttcga tccgaaacac
    17101 aaaatcggtc ttgcccccgc cggcaggctc gatatcgatt cagtcggatt gctggtgttg
    17161 acgcaagacg gccgtatcgc caagcagctt atcggcgaaa acagcggcag tgaaaaagaa
    17221 tatttggtgc gcgtgcgcgg caaattggac gaaaaaggac ttgccttact gaatcacgga
    17281 ttgagtttgg acggtgataa actgcgtccc gccaaagtag aatggcaaaa tgaagaccaa
    17341 ctgcgcttcg tgttgaaaca gggtaaaaag cggcaaatcc gccgtatgtg cgaactggtc
    17401 ggactgcgcg tcgtcgggct gaaacgcatc cgcatgggca aggtcaaact cggcaggctg
    17461 ccgcccggca aatggcgtta tctcgctccc ggcgaatcgt tttaaataag cattaaaaaa
    17521 tgccgtctga aaatccttgc gttttcagac ggcactgcct tatgcccaaa taacggtttc
    17581 ttccaagggc ctgcgcgctt tcgtgacgat ttcctgaacc cggtagccga atgtcgcggc
    17641 aacggacacg ccccattctg ccgcatcgaa caaaccgaac tgcccggaca atacgcgttc
    17701 catatcggca tagttgaaac cttccaccgg gcaggaatcg atacccgcca tcgccgcacc
    17761 cgtcatcatg ttggctaacg cgatataggt ctggcggcag caccagtcaa acaaggcgcg
    17821 cgaatcgtcc aaaatcttga tgtcgtcagc ttgaaacgcc tgatacctcg ccaaagattt
    17881 ttctacggca tccggttcgg taacgccgcg ccgtttgaga ctttccaaca taaacgggct
    17941 gtcgaagcgg gcatttttct tcgccaaaaa caccaccaaa tgactggcgg tatccaaagc
    18001 atccgccata ccccaagaaa acagcttgat tgcctgtcgg atttcggggt tttgaaccac
    18061 aacaaaccgc caaggctccg aaccgaccga actgggcgac aaacgcccga gttctaaaat
    18121 aaactgaaaa tcctcggcac tgattttacg tgccgcatcg taatgccggc acgatttgcg
    18181 gtttttaaat gcggatagaa cctgctcttt gcttaatact gtcatcgtgc atccttttat
    18241 tactttgatt gcaaattgat ttcaaaccga tacggcactg ccgcacccta taagccgtcc
    18301 ccgccatcaa gcttcacccg gattttaccc atctctctac ggctgggaaa acaaagcggc
    18361 ggcagattcg ggaataactc cccaatgcct ttcagacggc atttggacat cgcaaacgcg
    18421 cctacgggta ggcgatatag gcataagccg aatggttgtg gatagactcg aaattctcgc
    18481 tctcgacgac gaaactcttg atgcgtttgt cggcaatcag cgaagtagcg acatcgcgca
    18541 ccatatcttc cacgaatttc gggttttcgt aggccttttc ggtaacgtat ttttcatcgg
    18601 ggcgtttgag caggccgtag agttggcagc tcgcctgcgt ttccacataa tcgataactt
    18661 cctcgatacc gacttcggca tcggaagtca ggctgacggt aacgtgcgaa cgctgattat
    18721 gcgcgccata ttgggaaatt tctttggaac acgggcaaag cgaggttacg ggaatcatga
    18781 ccttcatact gtggccgtat gccccgtctt tcatttcacc cgtgaggctg acatcataat
    18841 ccagtaagga ccggataccg gataccggcg cggttttctt gcggaaaaac ggaaaagaaa
    18901 cgctgatttt gccggcgcgg gaatccaaaa gcgcgaccat ttcggcagtc agcctatgca
    18961 attgtgcgaa atccaagact tcggtatgtt gctccatcaa tgcgacaaag cgcgacatat
    19021 gcgtcccttt ctgctcggcg ggcagataaa ccgtcatcgt caggcgagca accgtggatt
    19081 gtgttccttc ggcggttttc agggtaatcg ggaagcgcag gtctttaatg ccgacctgat
    19141 tgatcggcag gttgcgcaaa tctctgctgg attgcacgtc tgcaatggcg ttcatgcgtt
    19201 gtttgtcctt aatattcaga taattattga gatgtgtgta ttgtatggca ggcagatgcc
    19261 gtctgaaaaa acgctgtcgg ccgcctgcct gcaaatgcga gattatatca cttgcttttg
    19321 gcggctgcat tgattgtttc tattttcggg atagtgataa aatcccgact tatccatatc
    19381 ttacaaggca gaaacagatg aaattcccga cccgtgccat ccattccggc tacgattgcg
    19441 acgaacacaa ccgcgcgctg atgccgccga tttatcaaaa cagtatgttt gcgctgcacg
    19501 aaatcggcga aaatgtgcct taccgttatt cgcgcctgag caacccgacc cgtcagattt
    19561 tggaagacac cgttgccgat ttggaacacg gtgcggcagg ttttgcgttt tccagcggta
    19621 tggcggggat tgatgccgtg tggcgtacct tcctgcgccc gggcgatacc atcgtcgcgg
    19681 tcgccgatat ttacggcggc gcatatgact tattggtcga tgtttatcaa aaatgggggg
    19741 tgaacgttgt ttttgccgat ttgggcaatc ccgataattt ggacgaattg ctcaaagcgc
    19801 acaatgtcaa actggtttgg ctggaaatgc cgtccaatcc gcttttacgc ttggtagaca
    19861 tcaaagccct tgcagcgaaa gccaaagcag ccgacgcgct ggtcggcatc gacaacacct
    19921 ttgccacgcc gtatctgcaa cagcccttgg atatgggttg cgattttgca ttccattccg
    19981 ctaccaaata tttgtgcggc cattccgacg tattgatggg catcgtcgtt gccaaaacca
    20041 aagagctggc tcagcctttg cacgatatga tggtgcatac cggcgcgatt gcaggcccga
    20101 cggactgctg gctggtgttg cgcggcatca aaacgcttgc cctgcgtatg gaggcacact
    20161 gcaaaaacgc actcgaaatt gcgcgccgtt tggaagccca tcctgccatt gaaaaagtgt
    20221 tccatcccgg cctgccgtct cacgaacatt acgcactcgc ccaagcgcaa atgcccaaag
    20281 gcatcggcgg cgtggttacg gtttatctca aaaacgacac gcgcgaagcg gcaaacagcg
    20341 tgattaaaaa catgaaactg gtcaaaatga cctccagcct cggcggcgtg gaaagtctgg
    20401 tcaaccattg ctattcccaa tctcacagcg gcgtgccgca taatgtgaaa atggaaatgg
    20461 gcatcagggt cggactcctg cgcttctcca tcggtattga agacgtagat gatatttgga
    20521 acgatatttc cgccgcactc gatacaactt tgtaaaccgt aaaaaatgcc gtctgaaacc
    20581 atagtttcag acggcatttc aatattgcga atcaaatatc aacgcttcag catctttgcc
    20641 gcctcaatgg cgtaataggt caaaatcccg tccgcacccg cacgtttgaa tgccagcagg
    20701 ctttccaaaa ccactttgcc gccgtccagc cagccgttgg caaccgccgc ctgcaacatc
    20761 gcatattcgc ccgaaacctg ataggcataa gtcggtacgc cgaactcgtc cttcacgcgg
    20821 cggacaacgt ccaaatacgg caaaccgggc ttcaccatca ccatatccgc accttcctga
    20881 atatcgagcg ccacttcatg cagcgcctca tcggtatttg caggatccat ctgataggtc
    20941 tttttatctg cctttcccaa attgcccgaa ctgcctaccg catcacggaa agggccgtag
    21001 aatgcagaag catatttggc ggaatatgcc ataatccgcg tatggatatg tccggcatcc
    21061 tccaaagcct cgcggatggc gccgatacgc ccgtccatca tatcggaagg agcaacgacc
    21121 tgcgtgcccg cctctgcatg acataaagcc tgtttcacca agacttctac ggtttcatca
    21181 ttcatcacgt aaccgttttc gtccgtcagt ccgtcctgac cgtgcaccgt ataaggatcg
    21241 agcgcgacat ccgtcataat gcccagttcg ggaaacctct cgcgcaaggc tcggacagtt
    21301 gacggcacga gtccttcggg attgtacgcc tcctgcgcac gcccggtttt gtttgccgta
    21361 accacgggaa agagtgccaa catcggaata ccgagcttca ccgcctcttc cgccgtaaac
    21421 agcagcctgt ccaaactctg acgcttcacg cccggcatag aaggcacatc ctcctcgcgc
    21481 gccgccccct ccaatacgaa caccggataa atcaaatcat cggcggtcag catatgctcg
    21541 cgcatcaggc ggcgtgaaaa atcatccctg cgcatacggc gcatacgcga agccggaaca
    21601 ttgcggtaag gaaattgcat aagccctcca atcatcctgt caacaattca aatcatacgg
    21661 aagccgccgc aacttgcaga cggcatcgag cggtaatcaa aatccgtttt caccggaaag
    21721 ggtttcggca cgccgaaatg ccgcgccgaa ggacagcgtg ccgcccattg aaagaacaat
    21781 agcaaaacaa ccacttcagg ctgtcaggcc tcgatattct tacgccacaa taccaaaagt
    21841 ttcccgatat gccgaacagg ttgcgcgtca accgcctcac acaaggtatt gcagatttcg
    21901 atacgctcgg cacggtcgtc gccgaatacg cgcactttaa tcagctcatg cgccgtcagt
    21961 gccgcatcgg tttccttgat gactgcgtcc gtcagaccct gctgaccgac catcacaaca
    22021 ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt ctttggtgtt caatttggta
    22081 tccgtcattt tcctactgtt tgaataaaaa ggataatgca gcattgtacg cgatttggca
    22141 cggcaacggc aaaattaacg tacaatacac cgtttacatt ccgacccgcg aaccactatg
    22201 gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac acatcaacga ccactacgtc
    22261 catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat acaaactttt ggaaatcaac
    22321 gaaaaagaca agataatcaa acccggcacg gtacttgccg acttgggcag cgcgccggga
    22381 agctggtcgc aggttgccgc caagctgacg ggtacttccg gaacggtttt cgccttggac
    22441 atcctgccta tggaagccat agggggcgtg tccttcattc agggcgactt ccgcgagaac
    22501 gacgttttgg cgcaattcga aaccttgttg gacaaccgcc cgctcgacct tgtaatctgc
    22561 gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc aggcacgcag cttttatctg
    22621 tgcgaactgg ctttggactt cgcctcgcaa cacctgaaaa ccggcggcag ctttttggtc
    22681 aaagtctttc agggtgcagg ctatcaggaa tacatggcag ccatgcgcga aattttcggc
    22741 actgtgcaga cgcgcaaacc cgaagcctcg cgcaatcgct ccagtgagat ttatttattg
    22801 ggcaaaaata aacgctgaca atacagacgg cgtgctttac aatcatttcc gttttacacc
    22861 tcaattatgg agccttgcta agtggggaac acctttaaat caatccttgt ctgggtcgcc
    22921 ttgggtatcg gcctgatggc tgcgttcaac gctttggacg gcaaaaaaga agacaacggg
    22981 caaatcgaat attctcagtt catccgacag gtcaacaacg gcgaagtatc cggcgtcaac
    23041 atcgaaggat ccgtcgtcag cggctacctg attaagggcg agcgcaccga caaaagcacc
    23101 ttcttcacca acgcgccctt ggatgacaac ctgattcaaa cccttttgaa caaaaacgtc
    23161 cgcgtaaaag taacgccgga agaaaaaccg agcgcgctga ctgccctgtt ttacagcctg
    23221 ctgcccgtcc tgctgctgat tggcgcatgg ttctacttta tgcgtatgca ggcgggcggc
    23281 ggcggaaaag gcggcgcatt ctccttcggc aaaagccgcg cccgcctgct ggacaaagat
    23341 gccaacaaag ttacctttgc cgatgtcgcc ggctgcgacg aagccaaaga agaagtgcag
    23401 gaaatcgtcg attacctcaa agcaccgaac cgctatcaaa gcctcggcgg tcgtgttccg
    23461 cgcggcatcc tgctggcggg cagcccggga accggtaaaa cactcttggc gaaagccatt
    23521 gcaggcgagg ccggcgtgcc gttcttcagc atttccggtt ccgattttgt cgaaatgttc
    23581 gtcggtgtcg gtgcaagccg cgtccgcgat atgttcgagc aggcaaagaa aaacgcccca
    23641 tgcattatct ttatcgacga gattgacgcg gtaggccgcc aacgcggcgc aggtttgggc
    23701 ggcggcaatg atgagcgcga gcaaacatta aaccaattat tggttgaaat ggacggtttt
    23761 gagagcaatc agactgtaat tgtgattgcg gcaaccaacc gccccgacgt actcgatcct
    23821 gcgctgcaac gccccggccg cttcgaccgc caagtcgtcg tccccctgcc ggacatccgg
    23881 gggcgcgaac agattttgaa cgtccattct aaaaaagtgc ctttggacga atctgtggat
    23941 ttattgtccc tcgcgcgcgg cacgcccggt ttttccggcg cggatttggc gaacctggtc
    24001 aacgaagccg ccctgtttgc cggccgccgc aacaaagtca aagtcgatca aagcgatttt
    24061 gaagacgcca aagacaaaat ctatatgggt ccggaacgcc gcagtatggt gatgcacgaa
    24121 gacgaaaaac gtgcgacggc gtatcatgag tccggacacg cgattgttgc cgaaagcctg
    24181 ccctttaccg accccgtcca caaagtaacc attatgccgc gcggacgtgc gctgggtctg
    24241 acttggcagc ttccggagcg cgaccgcatc agtatgtata aagatcagat gttgagccag
    24301 ctctccatcc tgttcggcgg acggattgcc gaagacatct tcgtcggacg catctccacc
    24361 ggtgcatcaa acgactttga acgtgcaacc caaatggcac gcgaaatggt aacgcgctac
    24421 ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa acgaaggcga agtcttcttg
    24481 gggcgcagcg taacccgttc gcaaaacatt tccgagaaaa cccagcagga catcgacgcg
    24541 gaaatccgcc ggattttgga cgagcagtat caggttgcct acaaaatcct cgatgaaaac
    24601 cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat gggaaaccat agaccgcgat
    24661 caggtactgg aaattatggc gggcaaacaa cccagcccgc ccaaggatta cagccacaac
    24721 ctgcgcgaga atgcggacgc ggcggaagat aacgcgccgc acgctccgac tcgggaaaaa
    24781 accgaagcac ctgccccggc agacaccgcc tcgacagagt ccgggcagca gcctgaaaac
    24841 aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc cgttttccca tcccgttttc
    24901 agacggcatt ttccgctcaa tgccgtctga aacgccaaag ccgtatcggt ttttgcgaat
    24961 ctgcgatatg gcggtttaaa ttgaaatcag aacggggcaa ggctgtaccg gtttaaaatt
    25021 aaaccgctat aataccgccc ttcgaataca cgcccgcatt ggtgatgccc gtgaaaaaag
    25081 ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat gttcgagctg gtcgacaagg
    25141 ttgaggatta cccgcacttt ctgccgtggt acagcaagac cgaggtcatc gggcgtagcg
    25201 gcaacgaact gaaggcgcgg ctgtttatgg attatatgcg cgttcgccaa tcgtttgcca
    25261 cgcacaaccg caacatcccc ggcagggaaa tccgtatgga actgctcgaa ggcccgttca
    25321 aaaccttacg tggaacgtgg aaatttatcg atttgggcga cgatatgtgc aaaatcgaat
    25381 tcaatttgga atacgatttt tccaatgccg ttttgtccgc cttaatttcc cccgtcttca
    25441 accacctttc cgccacgctg gtcgaagcgt tcgtcaaaga ggcagaccgc cgttatgctt
    25501 gaaattgaga ttgtgtacgg tctgcccgac cgacaggttt tgaaaaccat gcagcttgcc
    25561 gagggaacaa ccgtccgcac cgccgcactg caaagcggtt tggacggcat atttgaaaat
    25621 ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag ccgtcaaaga cgacacgccg
    25681 ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt tgatcgaccc caaagaagcg
    25741 cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc gtctgaagcc ttcagacggc
    25801 attcgacaga aacacggaaa atatcatgtc aaacacaatc aaaatggttg tcggcttggg
    25861 caacccgggc aaagaatacg aacagacccg ccacaatgcg ggcttttggt tcctcgacga
    25921 actggcgtgg aaatggaagg cttcgtttaa agaagaaaaa aaattcttcg gcgaagttgc
    25981 ccgcgccgcc ctgcccgacg gcgatgtttg gctgctcaaa ccggccacgt tcatgaaccg
    26041 ttccggacag gcggttgccg cgcttgcaca gttctacaaa atcaaacccg aagaaatcct
    26101 cgtcgtccac gacgaactcg acatcccttg cggacggatc aaattcaaac tcggcggcgg
    26161 caacggcgga cacaacggct tgaaagacat tcaggcaaaa ctcggcacgg cagactatta
    26221 ccgcctgcgc ctcggcatcg gccaccccgg cgaccgcaac ctcgtcgtcg gctacgtctt
    26281 gaacaaaccg agcgcggaac accgccggca aatcgacgat gccgtcgcca aatccctgca
    26341 ggccgtaccc gacatcattt ccggcaaatg ggaagaggca acgcgcttcc tgcacagcaa
    26401 ataatccaat gccgtcttaa gccctttcag acggcatttt cccgatttcc gtatccgaac
    26461 agtcatgacc gaactcaagc agcttatcca aaccgaatcc atccccgtca tcgaagaaac
    26521 cctcgatttc ctgctgtacg aatgcagcat cgacgacgca ccgtccgccg aagaagtggc
    26581 acaatggcgc gacatacttg ccgcacgcgg cggcaaattc ctgcgcctgt ccaaaatctg
    26641 ccaaacgtgg ctggacgagg aggcggcatg aagctgccgc gcaaccgctt cagcctgctt
    26701 tccgcattgt ggtttgccgg cggcatctat tcgctgctct tcaaagctgc cgacaccgcg
    26761 ccgccgccgt ttccacattt cgacaaagca gcacaccttg ccctgttttt cgcacaaatc
    26821 ttgcttctgg ccaaagcatt caaaaccgga aaacttccca tcccctaccg cagcctgatt
    26881 gcgttcgcct tctgttttgc cgtcggcagc gaatgcgcgc aggcatggtt tactgcaacg
    26941 cgaaccggca gtttgggcga tgtccttgcc gacctgacgg gcgcagccct tgccctcttt
    27001 gccgcgcgtt ctgcctgccg cccggactaa atcggtttat ttctcccaaa caggatgaat
    27061 ccccctgatg catcctgttt ttccccttac atatcaatcc catctcataa acaaaaccga
    27121 caaatcgttt acaatatatt tacactacac cgaattacaa atatactcga agcggttgca
    27181 gaaccgacgg tttcgcaaac cgtttcggat tgcacgacac aaaagaaaaa ccgattttgt
    27241 tgccttaaag gagcattcat gaacctgcat gcaaaggaca aaacccagca tcccgaaaac
    27301 gtcgagctgc tcagtgcgca gaagccgatt accgacttta agggtttgct gaccaccatt
    27361 atttccgccg tcgtctgttt cggcatttac cacatcctgc cttacagccc cgatgccaat
    27421 aaaggtatcg cgctgctgat tttcgttgcc gcactttggt ttaccgaagc cgtccacatt
    27481 accataaccg cactgatggt gccgattctc gccgtcgtac tcggtttccc cgacatggac
    27541 atcaaaaagg cgatggctga tttttccaac ccgattatct acattttttt cggcggcttc
    27601 gcgcttgcca ccgccctgca tatgcagcgg ctggaccgta aaatcgccgt cagcctgttg
    27661 cgcctgtcgc gcggcaatat gaaagtggcg gttttgatgt tgttcctcgt tactgccttt
    27721 ctgtccatgt ggatcagcaa caccgccacc gccgcgatga tgctgcctct agcaatgggt
    27781 atgctgagcc acctcgaccg ggaaaaagaa cacaaaacct acgtcttcct cctgctcggc
    27841 atcgcctatt gcgccagcat cggcggcttg ggcacgctcg tcggctcgcc gcccaacctg
    27901 attgccgcca aagccctaaa tctggacttc gtcggctgga tgaagctcgg cctgccgatg
    27961 atgctgttga ttctgccctt gatgctgctc tccctgtacg tcatcctcaa acctaatttg
    28021 aacgaacgcg tggaaatcaa agccgaatcc atcccttgga cgctgcaccg cgtcatcgcg
    28081 ctgttgattt tccttgccac agccgccgcg tggatattcg gctccaaaat caaaaccgcc
    28141 ttcggcattt ccaatcccga caccgttatc gccctgagtg ccgccgtcgc cgtcgtcgtc
    28201 ttcggcgtgg cgcaatggaa ggaagtcgcc cgcaataccg actggggcgt gttgatgctc
    28261 ttcggcggcg gcatcagcct gagcacgctg ttgaaaacat ccggcgcgtc cgaagccttg
    28321 ggacagcagg ttgccgccac cttttccggc gcgcccgcat ttttggtgat actcatcgtc
    28381 gccgccttca ttatttttct gaccgagttc accagcaaca ccgcctccgc cgccctgctt
    28441 gtaccgattt tctccggcat cgctatgcag atggggctgc ccgaacaagt cttggtattc
    28501 gtcatcggca tcgccgcatc ttgcgccttc atgctgccgg ttgccacgcc gcctaacgcg
    28561 attgtgttcg gcacgggctt aatcaaacaa cgagaaatga tgaatgtcgg catactgctg
    28621 aacatcctat gcgtagtatt ggttgctctg tgggcttatg ctgtactgat gtaaaccatc
    28681 gacctaaaca acaagaccgt ctgaaagaat atttttcaga cgaccttgaa gtttgtcgtt
    28741 cagacacaat ttgtcgaata attcaaaacc ggattctaac gaaaggaaaa cccatgatta
    28801 tcctgcacac caacaaaggc gacatcaaaa tcgaactcga tttcgacaaa gcccctgtta
    28861 ccgccaaaaa cttcgagcaa tacgtcaaag acggcttcta cgacggcgta atcttccacc
    28921 gcgtcatcaa aggcttcatg attcaaggcg gcggcatgga tgaaaacatg aacgaaaaag
    28981 aaacccgcga tccgattcaa aacgaagcgt ccaacggcct gcccaacgat aaatacacca
    29041 tcgccatggc gcgcacttcc gacccccatt ccgccggcgc gcaattcttc atcaacactg
    29101 ccgacaacgc tttcctaaac ttccgttcta aagagctgta cggcaaaacc gtcgtccaag
    29161 actggggcta tgccgtattc ggtaaagtcg ttgacggttt tgacgttgtc gatgccatcg
    29221 aaagcgtatc caccaaacgc cacggctacc acgacgatgt gccgaccgaa cctgtcatca
    29281 tcattaaagc cgaagcagta taaaccggca atccgaaagc agcctgcata aaaggctgct
    29341 tttatttcag acggcatgtt atttgatgta accgggctat gccgtctgaa aaccggacgc
    29401 aagccttcag acggcatacc gctatgttaa aatacgcccg tttttcctaa acggacacaa
    29461 aggaaacctt atggcaagca tcacccgcga catcttcaaa gcctacgaca tccgtggcat
    29521 cgtcggcaaa accctgaccg acgatgccgc ttatttcatc ggcagggcca tcgccgccaa
    29581 agccgccgaa aaaggtatcg cccgcatcgc gatcggacgc gacggacgct tgagcggccc
    29641 cgaactgatg gagcacatcc aacgcggcct gaccgacagc ggtatcggcg tactcaatgt
    29701 cggcatggtt accactccta tgctctactt cgcagccgtc aacgaatgcg gcggcagcgg
    29761 agtgatgatt accggcagcc acaatccgcc cgattacaac ggtttcaaaa tgatgctcgg
    29821 cggcgacacg ctcgcaggcg aagccattca agaactttta gctattgttg agaaagacgg
    29881 ttttgttgcc gccgacaaac aaggcagcgt aaccgaaaaa gacatctccg gcgcatacca
    29941 cgaccacatc gtcggacacg tcaaactcaa acgcccgata aacatcgcca tcgacgcggg
    30001 caacggcgtg ggcggcgcgt ttgccggcaa actctacaaa ggtttgggca acgaagtgac
    30061 cgaacttttc tgcgaagtgg acggcaattt ccctaatcac caccctgatc cttccaaacc
    30121 ggaaaacctg caagatttga ttgccgcgct gaaaaacggc gatgccgaaa tcggcttggc
    30181 gtttgacggc gatgccgacc gcttgggcgt ggttaccaaa gacggcaaca ttatttatcc
    30241 cgaccgccaa ctgatgctgt tcgcccaaga cgttttgaac cgcaatcccg gcgcgaaagt
    30301 cattttcgat gtcaaatcca cacgcctgct tgccccgtgg attaaagaac acggcggaga
    30361 agccataatg gaaaaaaccg gccacagctt catcaaatcc gctatgaaaa aaaccggtgc
    30421 actggttgcc ggcgaaatga gcggacacgt tttctttaaa gaacgctggt tcggcttcga
    30481 cgacggcctg tatgccggcg cacgcctctt ggaaatcctg tccgcctccg acaatccgtc
    30541 cgaagtgttg gacaacctgc cgcaaagcat ttccacgccc gaactcaaca tctccctgcc
    30601 cgaaggcagc aacgggcatc aagttatcga agaactcgcc gccaaagccg aatttgaagg
    30661 cgcaaccgaa atcatcacca tcgacggcct gcgcgttgaa tttcccgacg gcttcggtct
    30721 gatgcgtgct tccaatacca cgccgatttt ggtgttgcgt tttgaagcgg atacgcaagc
    30781 agccatcgag cgcattcaaa accgattcaa agccgtcatc gaaagcaatc cgcatttaat
    30841 ctggcctctg taaaaataga aaaaatgccg tctgaaactt tcagacggca tttttcacaa
    30901 gtagccaatc aaatcttggc agattggatt tggcttaaaa atctccgcgt ccgttcgtgt
    30961 ttgaggtggt cgaacaactc tttcgggctg ccctgctcta cgataacgcc gccgtccatc
    31021 acgacgacgt tcgtggcaac ttccagcgtg aacttgattt cgtgggtaac gacgaccatc
    31081 gtccaacctt cccgcgccaa ttccttcatg gcgtccaaca cgtcttgcac caactcgggg
    31141 tccagcgcgg aagtgggttc gtcaaacagc atcaattcag gctgaatcgc cagtgcacgg
    31201 gcgataccga cacgctgctg ctgaccgccg gaaagctggt agggatacaa atccacttta
    31261 tcgcccaagc cgactttttc cagcagtttc aaagcctctt cgcgcgcttg ggcggcaggc
    31321 ttgccctgta cggcaaccgg cccttccatc acgttttcca acacggtttt atgcgggaag
    31381 aggttgtatt gttggaatac cattccggac ttgcggcgca gtgccaaaat atcgtgtttg
    31441 cttgtttttt tggaaaaatc aatgcgtaac ggccgcgcgt tgtcgaactc gatttgtccg
    31501 tcttcgggca tttccaacgc gtttaggcag cgcagaaatg ttgttttacc cgagccggaa
    31561 ggcccgagga tgacgaccac ctgccctttg cccacatcca aatcgatgcc gcgcaaaatg
    31621 gtgttttcgc caaaggtctt atggatattg cggattttaa tcatgacaac tccttatttg
    31681 gcgacataac ggtcgaaacg tttttccaaa cgcgcctgaa tcaaaaacag cactttacag
    31741 aaacaccaat aaaccaatgc agcttcgata tagacaggca aaaagtcata agtgcggttt
    31801 gccgtttcct gtgcgacacg gaaaagctcc gttaccgtta ccacggcggc aagcgaggtg
    31861 tttttgaaca agccgataaa ctcgttgctc aacggcggaa cggcgacgcg gaatgcctgc
    31921 ggtgcgacga tgcggcggaa cgtctgcata taggtcatac cgatggagaa acctgcttcc
    31981 cactgccctt tcggcacgga caaaatcgcc gcgcgtatgg tttcggaagc gtatgcgccg
    32041 acattgagcg aaaagccgat gatggcggcg ggaatcggat tgatatagat gccgacggac
    32101 ggcagcccgt aaaacacaat cacaagctga accaacagcg gcgtaccgcg aacgacggaa
    32161 atataaaatt ccaccagctt caacaagcat ttttggaaaa taccgccgga aggcatgatt
    32221 cttaccaaag caacggctac ggcaataatc atgccgataa cgaaagaagc gatcgccaaa
    32281 ggcaaagaca ctgtaaagcc ggctttaacc atgggccaaa acgcgctgat gagcatatca
    32341 gcgcgtgttt ccgtcataaa cggcagagag gcaaggaaat tattgaacac tgatgtcttt
    32401 tccgaagaat tgttcgccca gttttttcaa cgtgccgtcg gctttcagct cgttgattgc
    32461 cgtgctgaat ttcgccacgg cctcgtcatt gcccttgttg acaatcagac cggaaccgac
    32521 tttttcatcg gcaggcgcgg accacacgat tttcaccccc gcattcgggt ttttcttcag
    32581 atagtccaaa accgccaatt catcgttcaa cgtcgcatcg gcgcgttttt gttcaatcag
    32641 ggtcagcgat tgcgccaaac cgtccaccgg cacgagttgc gcacctgcgg ctttggcttt
    32701 ttcgccgtag ttgctggtca gggattgcgc ggttttcacg cctttgatgt cggctatgga
    32761 tttaatgttg ctgtcgttac gcgcaaccaa aaccgcaccg ctccagctgt aaggttcgga
    32821 tttgtcaaat gtcgcctggc gttcggggct ggtcaggccg acttggtttg ccaccacgtc
    32881 gaaacgtccg gctttcaaac ccgccatcat cgaatcccat tgcgtttctt taaactcgac
    32941 ttttacgccc agtttttccg ccacggcgcg ggttacttcc acgtcgtaac cggtcagttt
    33001 gccgtctttg tcgtggtagg taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt
    33061 gcctttattg ttgatgcgct cgattaaaga accggaaatt gccgattgtg caggcgcgga
    33121 agatgctccg ctgccgcctt ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc
    33181 gccgagtacg aattttttca acatcattcc ctcctgtaaa aagtgtgcaa gtgcggcatt
    33241 ctaagtgaaa ttgcggcaaa cacaaagaat acttttttta tatggttatt ccaaatcgga
    33301 atgggaaaga tatgccgaag ccgccggaag cggaaataaa gggggcggga aaggcggcgg
    33361 gcggcacgaa accgaaaagc ggcatcaggt atgcaaacgg cggattcacg cacgccgttc
    33421 cggcaaaaat ccgcctgccg attcggtgct ataatccacc cgaaacagat gacaccaaaa
    33481 aggaacatct catgaacacg ccccaatccg ccatcattcc cgaccacgcc caagccggca
    33541 tctttatcga agccgacttc gccgccaacc gcctcaacga tattaaagcc gcctgccgcg
    33601 cttcgctcga cgcgttatcc gccttgaaag cccgctttcc ggacgatatt ttgggtctga
    33661 ccatcgcctt cggcagcaaa gcttgggcaa cattcggaca cacggacgaa ggcagtgaaa
    33721 tcaaaccctt ccccgaaatg ggcaacgggc ttgcgccgtc cacgcagcac gatatgtcca
    33781 tccacatcca atccttccgc caaaacgccg cctacgcgct tgcccaatcc gttttgggcg
    33841 cgttcggcga cagcatatgc gtcgcgtccg aagaacacgg tttgcgcctg tatcaggatc
    33901 gcgggctgga cggtttcgtc gacggcacgg aaaacccgca aggcgatgaa acaatccgcg
    33961 aagtcgccat catccccgaa gggctgcccg atgcaggcgg cagctatgtc ctgctgcaaa
    34021 aatacctgca cgatttgaaa aaatgggatg ccgtccccgt cgccgaacag gaggcctcgg
    34081 tcggacgcag caaggaaacc gacgacgaat tcagccgcga tgtccgcctg cccgattcgc
    34141 acctcggccg cgtcaacctg aaagaaaacg gagtcggcct gaaaatcgtc cgccgcagcc
    34201 tgcccttcgg caaaatcagc ggcgaacacg gtttgatgtt taccgcatac tgccgcacgc
    34261 tgcataatat cgaagcgcaa ttattgagta tgttcggcga cacggacggc aaaaccgacc
    34321 tgctgctccg acacctctcc gccgccgttt cgggcggata ctactacgcg ccgtctgtcg
    34381 aacgcctgca aaacctctaa gccggacgat gccgtctgaa accctttttc agacggcatt
    34441 ttcccgtttt accgtccaac cgttttcata cacgctatgt ccgcacccat ccgagcattc
    34501 gacccgctga ccgcccccat ttccggcacc aacctgattg aggcctctgc cggcaccggc
    34561 aaaacctacg gcattgccgc cctgtttacg cgcctgatcg tattggaaca aaaaagcgtc
    34621 gagcgcgtat tggtcgttac cttcaccaaa gccgccactg ccgagctgaa aacacgcctg
    34681 cgcgcgcgtt tggacgatgt gttgcaggtt ttggaaagca aagaaattgc cgaacttgga
    34741 gacgacacgc tttcagacgg cattgccgcc tactgcgccg aacaccacga aggcgacacc
    34801 ttcctgcccg cactcttgga acaggctttg caaaaagaaa gccggacgcg tctgattgtc
    34861 cgcctcaagg ccgccatcgg gcaattcgac aacgccgcca tctacaccat ccacggcttc
    34921 tgccagcgca tcctgcgcga ctacgccttc ctgtgccaag caccgttcga tgtcgaactg
    34981 accgaagaag acggcgaccg cctgcttgtc ccggctcaag atttttggcg ggaacgcgtc
    35041 agcggcgacc cggtgcttgc cgcattggcg tttaaacgca aagctgtgcc gcaaaccgtc
    35101 cttgcccaaa tccgcgccta cctgtcccgc ccgtacctga atttccgccg tccgcaggcg
    35161 gatttgaaac aggcgcagcg cgacgccgaa acctcatggc aaaccgtctg ccgcctgctg
    35221 cccgagctgg aagccggctt ttggcgcatc catcccgacc tcaacggcaa cagttaccgc
    35281 aaaaacagct tcggcaacct ttttaaagaa ctggcacaaa aatccgccgc cggacaactg
    35341 ccctttctgg acaaagacac gcacgaacgg ctcttgaaac tctcatccga caaactcgaa
    35401 gccggactga aaaaaggcaa aacgcccgat gcggcagtat ttgccgaatt gcagaaactg
    35461 gcagacttcg ggcgcgattt gaacgcactc gaagaagcgg aagaagcaac aatgatccgg
    35521 ctgcaactgg atttaatcga atatctcaac agcagccttg ccgaaatgaa aaaatcgcgc
    35581 cgcgaacgcg gtttcgacga cctgctgctc gatgtccaca ccgcgctgac cgacaatccg
    35641 cacgccgaaa cccccgcccg cgccgttgcc gaaaactggg aaatcgcgct gatcgacgag
    35701 ttccaagaca ccgacccgct gcaatacgaa atcttccaaa agattttcat cgcccgaaac
    35761 cgcccgctgt tcctcgtcgg cgaccccaaa caggcgattt acagctttcg cggtgcggac
    35821 atttacgcct accttcaggc ggcggaagac gcgcggcacc gctacacgct cgccaccaac
    35881 taccgcagcc acgccgcgct tatcggcagc atcggcgcgc tgttccgcct caaagaacgc
    35941 ccgttcgttt tggaaaacat cggctattcg gaagtcggtg cggcgcgtgc cgaaagcagg
    36001 ttgtcgccca aacgtcctgc cgtacagctc cgttggctgc acgaaaacga caatgaaaaa
    36061 gccaacaaag acgttttgcg ccgccgtgcc gccgactatt gcgccgacga aatcgcccac
    36121 gcgctcaacg aagcctccgg aggccgtctg aatttcaaag gccgcccgtt gcagtcgggc
    36181 gatattgccg ttctggtacg cacgcacaac gaggcggtga tgatttccgc cgcactgaaa
    36241 aaacggcagg tgcaaagcgt cctgctttcg cgcgaatccg tgttcgcctc gcccgaagcc
    36301 gccgccctgt ccgcgctcat cggcttctgg ctcgaaccgc gccgcgccgg aacgctgcgc
    36361 tttgtcctga caagcggcat attcggttat gacgcgcagc aattgcacga cttcaaccaa
    36421 aacgaaagcg agattttgca ttgggcggaa tctgcccgaa ccgcgctcga catatggcaa
    36481 aaatacggca ttttcgccgc tatgcagcag ttttcccaaa cacacggcat cgaaacgcgc
    36541 ctcttaagcc ggaacaacgg gcgcagcctg accaattatt tccaactgct cgaactgctt
    36601 gccgccgaag acgcgcaaaa ccgcaacccc gccgcactgc acaaatggct gcgcgatcaa
    36661 atcagcctgg ccggcaacaa cggcggcgac aaccgcgcca tccgtttgga aagcgacgaa
    36721 gatttggtca aaatcgttac catgcacgcc tcgaaaggtt tgcagtatcc gctggtgtac
    36781 tgcccgtttg cgtgggacgc gcaagatacc ggaccgtccg actggcaaat cctccaccaa
    36841 ggcgcaaacc gggccgaact tttggcaaag gcgcaactgt cggaaagcga acagaagcaa
    36901 tacgccgacg aagaaatggc ggaacgcctg cgcctgcttt atgtcgcgct gacgcgtgcc
    36961 gaggaacagc tcaacatcta cgccgcctat tcttccgata ccgccgacaa cccccttgcc
    37021 tacctgatcg aaggcttgcc gcaagacagc cgcgaaaccg tccgccgtac ctatgcgtgt
    37081 gaaaaagacg gcataacgat gctcaaacgc aactggcggc gcgtggcgga caatgccccg
    37141 gccggcacgg atttcgcctt cacagaagat gcgccgcccc ctgccgccta tcgcggcaac
    37201 gccgatcaag ccgccgaatt tgccgcaaac agcattcccg aacgcggatt ccgatttgtc
    37261 cgccacacca gcttcaccgc cttaagccgc caaacccaaa cgcccgacgg cggcgaagaa
    37321 gatgcctgcc cgtccttgga tgccgccgaa acctcggtgc cggcgatgcc gtctgaaacg
    37381 ccgacggctt cagacggcat atcgatacac gactttccga aaggcacgca ggcggggctg
    37441 tgcctgcacg aaattcttga agatttcaaa ttcggacaag cagccgccga acaggaaacc
    37501 ctcattgccg acaagctgaa aaaatacggt tttgaagaaa tatggctgcc cgccgttgcc
    37561 gaaatggcgg aagcctgccg caaaacgccg ctgactgggg catacggcct gtccgacatc
    37621 ccgcccgggt gccgccgtcc cgaaatgggc tttaccctcc acaccgaaga cttcggcctc
    37681 aaacggctgc gcgactggct tgcccgcgac gacatcaggc tgcccgaagt ctgccgtgcc
    37741 gccgccgaga cgctcgactt ccacaccgtc aacggctttt taaacggctt tatcgatatg
    37801 gtctgccaag accccgacgg caatatctgc atcatcgact acaaatccaa ccacctatcc
    37861 gcctacacgc ggcaggcgat ggacgaagcc gtcgcgcacc agcactatta ccttcaggca
    37921 ctgatttacg ccgttgccgc cgcgcgctac ttcaaactgc gcggacaacc gcccgccgcc
    37981 gtttccgtcc gctacctgtt tttgcgcgga ttggatggca aaggcggcgg cgtttggcgt
    38041 tgggacatag atgccgccgc tttggaacag ataaaataac ttcccgtccc ttaagacgac
    38101 aaccgcagga gaaccctatg aatatcaaac aattgattac cgccgcactc attgcctcag
    38161 ccgcctttgc cacgcaggca gccccgcaaa aacccgtatc cgccgcccaa accgcgcaac
    38221 attcagccgt ttggatcgat gtccgttccg aacaggaatt tagcgaaggt catttgcaca
    38281 acgcggtcaa catccccgtc gaccaaatcg tccgccgcat atacgaagcc gcgcccgaca
    38341 aagacacgcc ggtcaacctc tactgccgca gcggacggcg tgccgaagcc gcccttcaag
    38401 agctgaaaaa agcaggttat acaaatgttg ccaatcacgg cggttatgaa gacctgctca
    38461 aaaaagggat gaaatgacgc aatgccgtct gaaaggacag gctttcagac ggcatacggc
    38521 gttccggcac gcggcacggc atcaggcgcg gcgcgtgccg ccccgtccga agcccgataa
    38581 acaaaccgat aaggaaatac gatgaaacac atactccccc tgatcgccgc atccgcactc
    38641 tgcatttcaa ccgcttcggc acaccctgcc gacaaaccgc ccacccaaaa cgaaaccgct
    38701 atgaccacgc acaccctcac ctcgaaatac agttttgacg aaaccgtcag ccgccttgaa
    38761 accgccataa aaagcaaagg gatggacatt tttgccgtca tcgaccatca ggaagcggca
    38821 cgccgaaacg gcctgaccat gcagccggca aaagtcatcg tcttcggcac gcccaaggcc
    38881 ggtacgccgc tgatggtcaa agaccccgcc ttcgccctgc aactgcccct gcgcgtcctc
    38941 gttaccgaaa cggacggcaa agtacgcacc gcctataccg atacgcgcgc cctcatcgtc
    39001 ggcagccgca tcagtttcga cgaagtggca aacactttgg caaacgccga aaaactgata
    39061 caaaaaaccg taggcgaata agcccgtcgc cgacatatgg cggatggaaa tcaaatgcac
    39121 acgcccctca ttcccccgaa gtcgggaatc caagcggttg ggttaccgtg atttccaatc
    39181 attgccgaaa cggacgattc cggattcccg ccttcgcagg gatgacgggc gtatgcgttt
    39241 tgccatatca caggcaactt tgccgcaaat cttatctgtc gccggtatgc cttcttgttt
    39301 caatccgcca caaaccaaat gccgtctgaa acctgatttc agacggcatt tccgtttatt
    39361 ccctgctgtc gcggcaaata tcgatggctt cctgcaaact tgaaacgccg tggattttca
    39421 ggttcggaaa ctctttggca ttgcgcggca tattggcttt ggggacgatg gcgcgtttga
    39481 agccgagctt ttccgcttct ttgagccgct cttgtccgcg tgcgacgggg cggacttcgc
    39541 cgcttaggcc gatttcaccg aagaccacgg ttttttcagg catagggcgg ttgcggaagc
    39601 tggaaagcat cgcgaggatg accgccaaat ccgccgccgg ttcgccgatt ttcacgccgc
    39661 cgacggcgtt taaaaaaaca tcctgatcga aacaggcgat gccgccgtgc cggtttaaca
    39721 cggcaagcag catcgcaagg cggttttgtt ccaatccgac gctgaggcgt ttgggtgtga
    39781 agccgtgcgc gtcatcgacc aatgcctgaa tttcgaccaa aagcgggcgg ctgccttcct
    39841 gtgtaaccaa aacgcacgaa ccgggcgtgt cgtcgcggta gctggcgagg aagatggcgg
    39901 acgggttgga tacgcctttc aaaccgtttt cggtcatcgc gaacacgccc aattcgtttg
    39961 ccgcgccgaa acggtttttg atggcgcgta tcatgcggta gttggaatgt tggtcgccct
    40021 cgaaatacag cacggtatcg accatatgtt ccagcacgcg cgggccggca atcgctccgt
    40081 ctttggtaac gtgtccgacc agtatcatgg cgatacccat ttgtttcgcc atacgcgtca
    40141 gttgggcggc acattcgcgc acctgcgaca cggaaccggg ggcggacgtg atttggtcgg
    40201 aatacatggt ttggatggaa tcgataacga cgacttcggg ctgatgctgt ttcaaggccg
    40261 cctgaatcgc ttccatgcgg atttcggcaa gcaggtttac gccttcggcg tgcagttcca
    40321 aacgttgcga gcgcagggcg acctgttggg cggattcttc gccggaaacg tacagcactt
    40381 tgcggctttg cgccattttg gcgatggttt gcaacaacag cgtggatttg ccgatgccgg
    40441 gatcgccgcc gagcaggatg accgcgccat cgaccaaacc gccgcccaat acgcggtcga
    40501 gttcgcccat accggtcgga ttgcgcggca cttcggcagc ggtaacggcg gagagggatt
    40561 ggacggtcga agcgtccgcc gcccaagatt ggaatcgggc gtttttcggc tcgggcgcgg
    40621 caaagctttc ctgaagcgtg ttccactcgc cgcaatgcgg acatttgccc tgccatttcg
    40681 gggaagtgcc gccgcattcg gtgcattggt aaagggtttt aagcgttttt gccatgatgt
    40741 tttccctatg ggatttttga agtgtaaaaa aatcgtctga aacttctttt ctaaaaaatt
    40801 ttttattatt agtttgttaa ttttgattac aagaccatat cacgatacaa acaagggtgg
    40861 ctttctgtta ttttgcggca taatgacgga aaatacaaaa caggaatccc aaacaatgac
    40921 tttcccatta aatataatgg taga
//
LOCUS       DAWTHD010000004        37310 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWTHD010000004 DAWTHD010000000
VERSION     DAWTHD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37310
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.004"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            69..1193
                     /locus_tag="WM704_000137"
     CDS             69..1193
                     /locus_tag="WM704_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9339662.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            1190..2227
                     /locus_tag="WM704_000138"
     CDS             1190..2227
                     /locus_tag="WM704_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HFA9339663.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            2228..4117
                     /locus_tag="WM704_000139"
     CDS             2228..4117
                     /locus_tag="WM704_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9339664.1"
                     /translation="MLRTYLNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     SDKPAKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTV
                     YYSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKD
                     EIQSDLYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGR
                     KLSRATGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLV
                     YQSAK"
     gene            complement(4180..5244)
                     /locus_tag="WM704_000140"
     CDS             complement(4180..5244)
                     /locus_tag="WM704_000140"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFA9339665.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFAHA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMVQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(5319..6536)
                     /locus_tag="WM704_000141"
     CDS             complement(5319..6536)
                     /locus_tag="WM704_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFA9339666.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPENMRRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(6533..7867)
                     /locus_tag="WM704_000142"
     CDS             complement(6533..7867)
                     /locus_tag="WM704_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFA9339667.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(7879..8643)
                     /locus_tag="WM704_000143"
     CDS             complement(7879..8643)
                     /locus_tag="WM704_000143"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFA9339668.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPTAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(8661..9083)
                     /locus_tag="WM704_000144"
     CDS             complement(8661..9083)
                     /locus_tag="WM704_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339669.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(9365..10333)
                     /locus_tag="WM704_000145"
     CDS             complement(9365..10333)
                     /locus_tag="WM704_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9339670.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(10333..11157)
                     /locus_tag="WM704_000146"
     CDS             complement(10333..11157)
                     /locus_tag="WM704_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFA9339671.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(11187..11828)
                     /locus_tag="WM704_000147"
                     /pseudo
     CDS             complement(11187..11828)
                     /locus_tag="WM704_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11997..12317)
                     /locus_tag="WM704_000148"
     CDS             complement(11997..12317)
                     /locus_tag="WM704_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFA9339672.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(12340..12966)
                     /gene="upp"
                     /locus_tag="WM704_000149"
     CDS             complement(12340..12966)
                     /gene="upp"
                     /locus_tag="WM704_000149"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFA9339673.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(13072..13383)
                     /gene="grxD"
                     /locus_tag="WM704_000150"
     CDS             complement(13072..13383)
                     /gene="grxD"
                     /locus_tag="WM704_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFA9339674.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(13484..13699)
                     /locus_tag="WM704_000151"
     CDS             complement(13484..13699)
                     /locus_tag="WM704_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFA9339675.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(13725..14504)
                     /locus_tag="WM704_000152"
     CDS             complement(13725..14504)
                     /locus_tag="WM704_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFA9339676.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(14509..14859)
                     /locus_tag="WM704_000153"
     CDS             complement(14509..14859)
                     /locus_tag="WM704_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HFA9339677.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(14863..15840)
                     /locus_tag="WM704_000154"
     CDS             complement(14863..15840)
                     /locus_tag="WM704_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFA9339678.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(15874..16986)
                     /locus_tag="WM704_000155"
     CDS             complement(15874..16986)
                     /locus_tag="WM704_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFA9339679.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEI
                     SLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            17172..17873
                     /locus_tag="WM704_000156"
     CDS             17172..17873
                     /locus_tag="WM704_000156"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFA9339680.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            18015..19808
                     /gene="lepA"
                     /locus_tag="WM704_000157"
     CDS             18015..19808
                     /gene="lepA"
                     /locus_tag="WM704_000157"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HFA9339681.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            19954..20973
                     /gene="lepB"
                     /locus_tag="WM704_000158"
     CDS             19954..20973
                     /gene="lepB"
                     /locus_tag="WM704_000158"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFA9339682.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(21078..21185)
                     /locus_tag="WM704_000159"
                     /pseudo
     CDS             complement(21078..21185)
                     /locus_tag="WM704_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21409..21558)
                     /locus_tag="WM704_000160"
     CDS             complement(21409..21558)
                     /locus_tag="WM704_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339683.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(21604..22511)
                     /locus_tag="WM704_000161"
                     /pseudo
     CDS             complement(21604..22511)
                     /locus_tag="WM704_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(22820..23752)
                     /gene="cysK"
                     /locus_tag="WM704_000162"
     CDS             complement(22820..23752)
                     /gene="cysK"
                     /locus_tag="WM704_000162"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFA9339684.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTEIYDSITKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(23948..24553)
                     /locus_tag="WM704_000163"
     CDS             complement(23948..24553)
                     /locus_tag="WM704_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339685.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(24550..25401)
                     /gene="dapF"
                     /locus_tag="WM704_000164"
     CDS             complement(24550..25401)
                     /gene="dapF"
                     /locus_tag="WM704_000164"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFA9339686.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            25538..25852
                     /locus_tag="WM704_000165"
     CDS             25538..25852
                     /locus_tag="WM704_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339687.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            25849..26517
                     /locus_tag="WM704_000166"
     CDS             25849..26517
                     /locus_tag="WM704_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFA9339688.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(26590..28713)
                     /gene="pnp"
                     /locus_tag="WM704_000167"
     CDS             complement(26590..28713)
                     /gene="pnp"
                     /locus_tag="WM704_000167"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFA9339689.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKRLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(28947..29810)
                     /locus_tag="WM704_000168"
     CDS             complement(28947..29810)
                     /locus_tag="WM704_000168"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFA9339690.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPV
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            30003..30866
                     /gene="rfbD"
                     /locus_tag="WM704_000169"
     CDS             30003..30866
                     /gene="rfbD"
                     /locus_tag="WM704_000169"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFA9339691.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPHQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(31237..31470)
                     /locus_tag="WM704_000170"
     CDS             complement(31237..31470)
                     /locus_tag="WM704_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFA9339692.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(31636..31836)
                     /locus_tag="WM704_000171"
     CDS             complement(31636..31836)
                     /locus_tag="WM704_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFA9339693.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(32008..32883)
                     /gene="xerD"
                     /locus_tag="WM704_000172"
     CDS             complement(32008..32883)
                     /gene="xerD"
                     /locus_tag="WM704_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFA9339694.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(32935..33375)
                     /locus_tag="WM704_000173"
     CDS             complement(32935..33375)
                     /locus_tag="WM704_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFA9339695.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            33696..34634
                     /locus_tag="WM704_000174"
     CDS             33696..34634
                     /locus_tag="WM704_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFA9339696.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            34763..35056
                     /gene="hfq"
                     /locus_tag="WM704_000175"
     CDS             34763..35056
                     /gene="hfq"
                     /locus_tag="WM704_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFA9339697.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            35141..35707
                     /locus_tag="WM704_000176"
                     /pseudo
     CDS             35141..35707
                     /locus_tag="WM704_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            35761..36135
                     /locus_tag="WM704_000177"
     CDS             35761..36135
                     /locus_tag="WM704_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFA9339698.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            36145..36639
                     /gene="folK"
                     /locus_tag="WM704_000178"
     CDS             36145..36639
                     /gene="folK"
                     /locus_tag="WM704_000178"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFA9339699.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(36767..37246)
                     /locus_tag="WM704_000179"
     CDS             complement(36767..37246)
                     /locus_tag="WM704_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFA9339700.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
BASE COUNT         9451 a         9854 c         8678 g         9327 t
ORIGIN      
        1 agagcatagt tcacctttaa aatccataca aggattttta tcaaatttat gcataggagt
       61 gaaatcctat gctatctaaa caaatctcaa atcttaattc ttctagtaac aaaccaaaaa
      121 tcctatctct attttcagga tgtggcggtt tggatttggg ctttcatcaa gctggttgtg
      181 aaactgtttg ggcgaacgat ttctcccatt gggcttgcga aagtttccgt aaaaatatcg
      241 gcgatgtcat cgtagaaggt gatattgaac aaattaatcc gaatgatcca actattcccg
      301 attgcgacat cattttaggc ggattccctt gtcaagattt ttccatgatt tggaaacagc
      361 cgggcttaga gggtgagcgc ggcaatcttt ataaaagctt tttacgtttt gtaaatgcaa
      421 aaaaaccgaa agtttttgtt gctgagaatg tgaaaggttt attgactgcc aacaagaaaa
      481 aagccatcca gcaaattatt accgactttg aaaattgcgg ttattacgtt caggcgaagc
      541 tgtataactt tgcagaattt ggcgtacctc aatttcgtga acgtgtgctg attgtcggag
      601 tacgtttgga tacaggattt gattttcgcc atccggaacc gacgcacaat gaaactggcg
      661 aaaacggctt aaaaccatat gtaacagcag gtcaggccat atccaatatt ccacaaaatg
      721 ccagtaataa tgaattactg aaaatcagcg ataaaacacg ccgtatgttg gaattaattc
      781 ctgaaggtgg aaattttacc gatattccta aagatcatcc tttatatgtg aaaggtatga
      841 ttagccacgt ttatcgtcgt atgcatcgga acgagccatc aaaaacaatt attgcagcag
      901 gtggcggtgg tacttggggc tatcacttcc ctgaaccgcg tgcttttact aatagagaac
      961 gagcaaggct tcaaagtttt cctgatgatt ttgagtttgt cggatcaaca actgaagtac
     1021 gtcgccagat tggtaatgct gttcctcctc agggcgtggt tgaactggca aaaagcattt
     1081 taccgatttt ttcagacaac tatgagaaag tagatttgca tgagaaatta gtcgaagaaa
     1141 aagaaatttt attccatgac cgactaagca aaattcgagg gggaaaacaa tgaatacagt
     1201 tttttccaat atcgccaatg ccaaaatcac tgaaaaatct cttaatgcgg tttggatgga
     1261 tttatttaaa tcagcggatg aagtgttgat ggcgacaggt tatgtatcta atgacgcagt
     1321 ggttgagtta cataaaattt tggagctaaa cgatcatatt caaaaaatag atttattggt
     1381 cggaatgcat tatttggaag gttttagcca tttacaatat gacagcttat gtaaactaaa
     1441 tgattttttg cgacatgaga aaagaggagc cgtctatgtt tccccctttg tgaaatttca
     1501 cggcaaaatg tattccttta aaaattatca aaaaatcaat ggattaattg gttctgctaa
     1561 cttaacgtgt ttttgggata gcacggaacg tacttatgaa actatgttgc atttgaacgg
     1621 taagcctgca cagattttgc aggcagatat tcaaagtact attcataagc taggcaaaaa
     1681 tatccaagaa gttgaaagac caagtaaatt tattgaacat aatagccatt tagaaaattg
     1741 tttgggtgtt cagaaaattg caccagaaca gattcggcaa ttatttgccc aaacgtcaga
     1801 atatcatttc tcaattccag ctaaaaccga agaaaaaagt aatttgaatg tattttttgg
     1861 agaaggtcga cgagataaac gagggtttgt caaaccacga ccttggtatg aagtagagtt
     1921 gattgtatcc aaagacatca cttcacaaga agggtatccg gttctgaagt cttttacagt
     1981 tattaccgat gatggttggc aatttcaatg caagacatcg ggggattact ccaaaaactt
     2041 ccgctcagaa aatgatttaa agacacttgg taaatggatt aaaggtcgtt tggaaagtca
     2101 tggctgcctg caaaataacg agaagattac gcatgaaacc ctacgcgaat acggtaacga
     2161 tcattttgag ttacgttcaa ccgataatcc tgatgtttgg ctgctctcat ttaaggggaa
     2221 aaactaaatg ttgcgaacct acttgaacca attgacccct cccgaattag ctgattctgt
     2281 taaaaatacc gttgatggtt ttatggaaaa attgtcacaa accgagccga aaatcgccca
     2341 aaatgtttta ttgctgggca atgttcaaag tggcaaaacg gcacaggttt tgggtgtatt
     2401 aagtgcgctg gctgatgatg gcgaccacaa agtttttttg tatcttacta ctgacagcgt
     2461 agatttacag gatcaaacag tcaaacgagc caaagccaat ctgaaaaact ttatcgtatt
     2521 gtctgaagct gacgaccgaa gttttatgga agtaatgaag gcagagaacc ccatcttggt
     2581 cgtgattaaa aagaatgccc gtgtattgaa acgttggcgt aatttgtttg ccagtcaaag
     2641 tagcctgaaa ggctatcctt tggttattgt ggacgatgaa gccgatgctg ccagcctaaa
     2701 tactaattct gataagccgg ctaaagatgc cagtactatc aataaactgc taaacgacat
     2761 caaaaattcc tgttgtcaaa gcctgtttat ccagttgact gccacgcctc aatcgctttt
     2821 attacaacat gaagaatctg attggcaacc tgaatttatc cacttctttg aagcaggcga
     2881 aaaatacatc ggcggcaact ttgtcttttc tgatccacca agctatatag ttcgttttat
     2941 tgatagcgaa ttggatgata tgaaagacga gagtggtgaa attgccgaag gagcaaaaca
     3001 ggcattgctt agttttctaa taacctgtgc agaattcgcg ctatgtgata aggctaactg
     3061 taattttgca ctacatccaa gctataaaat tcaagatcat caagcctttt caaagaagat
     3121 ccaagccttt ttaaatgact tagtgcaagc agtcaataat ggggaagacc ttgcaggtag
     3181 ctttaaagaa agttatttgg atttgcaaaa gaccaagccc gatattcatc actttgatga
     3241 aatatacgaa aaattgacag cacttttaga gaataaacaa atctctactc ttgtcgttaa
     3301 ctcgcagaca gaaacagatt ttgacttaga aaaaggtttc aacatcatta ttggtggtaa
     3361 tgtgattggt cgtggcttga ctattccgaa actacaaacg gtttattata gtcgcactgc
     3421 caaaaaaccg aatgcagata ctttctggca acattcgcgt atttttggat atgaccgcga
     3481 taaatcatta ttacggcttt atattccgtt tgatgtctat tacttctttg tacaactcaa
     3541 tcaagcaaat aatctgatta ttgggcaggc aaaaaattca ggcggcaata ttcaggttat
     3601 ttatccgaaa aatatcaatc caactcgaaa aaatgtattg aaattcgata gtatcaatca
     3661 aattgtgggt ggtgtgaatt attttccact ccacccaaac gaagataatt tatcggagat
     3721 taacaaaatt ttgccttcta ttttgaaaga tgaaattcaa tctgatttat atcaaataga
     3781 tatagaagat ttattcttgg ttctagataa attgggtcgt tatgttccag atgactggaa
     3841 taaggaaaaa tttattgctg gtgtagaagc attaaaagca caacgtccaa gttttaaaac
     3901 ctatgttttg atcaaaaccg gacgtaaact ttctcgagca accggcacaa tgctttcgga
     3961 agacgaccgt aaattaggag aaaaatatcc caatgattta ttccttacgc tttatcaagt
     4021 agtaggaaat aaagacaaag gttggcaggg aaaagatttt tggttaccca atatcaagct
     4081 accacacaat ggcttggtgt accaaagtgc aaaatgaagc atatgccgtc tgaaaacttc
     4141 cgattcttca gacggcatat cgggcggttt cctgccggct tacccgccgt gatactgccg
     4201 cgacagctcg tgtacggtgt cgactaagat tttggcgtgt tcggggtcgg cgtgttggtt
     4261 gatgccgtgt ccgaggttga agacatggcc gctgccgtgt ccgtagccgg tcaggatacg
     4321 tgcgacctcg gtgcggatgg attcgggcgt accgaagagg gcggacgggt cgaagttgcc
     4381 ttgcagggcg acttggttgc cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc
     4441 caagcccaat gcgtctgcgc cgatttggac catgctttcc agccacagcc cgccgccttt
     4501 ggcaaacacg ataacaggca cgcggcgacc ttcgctttca cgtttgagtc cggcaacgat
     4561 ttggcggatg tatttgaggc tgaactcttt aaacgccgcg tcgctcaaca cgccgcccca
     4621 agtgtcgaaa atctgcaccg cctgcgcgcc cgcgtcgatt tgggcgttga ggtaggcggt
     4681 aacggcttgg gcgttggtat cgaggatttt gtacagcaaa tcggggcgcg agtacatcat
     4741 ggttttgatg gtgcggaatt ctttgctgcc gccgccttcg accatataac aggcgagcgt
     4801 gaacgggctg ccggagaagc cgataagcgg tacgcggccg tccaatgctt tacggatgga
     4861 agttaccgcg tcgaaaacgt attgcagttt ttccatgtcg ggaacgtgca gcttggcgat
     4921 gtcggattcg tgttgcaagg cgtgtgcgaa tttcgggcct tcgccttcgg caaaatacag
     4981 ccccaagccc attgcatcgg gaacggtcaa gatgtcggaa aacaaaatcg ccgcgtccaa
     5041 atcgaagcgt tccaaaggct ggatggtgac ttcggtcgcc aatccggtgt ttttgcacaa
     5101 atcgaggaag ctgcccgctt tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc
     5161 ctggcgcatc atccaaatcg gtgtgtattc gacgggctgt ttgagcaggg cgcggaggaa
     5221 ggtatcgttt tttaaaagag tcatacgggt tttctgtcag tctgtttgtc aagtgaaatg
     5281 gttttcagac ggcatttcaa cgatgccgtc tgaaggtttc aacgggtttc ggcggaaggg
     5341 cggttttccc cggcaacgct tgccaaaacc aagttgcgct gtgcttcggc tttttgcgac
     5401 tgtgcggttt cgtcaaaaac ctttgccaac accaaacgcg ccggaatact cggcttcagt
     5461 gcaatactcg cttcaaggta gccttttgcc ttaccccaaa gtttgcggcc gtaggcgagc
     5521 cggccgagat acatcagcag aagcgcgtta tcgggctgtt ctttcagcca agaatcggca
     5581 aaatcgatgg ctttctgctg ttcgcgctcg cccaaaaagc gcacgctttc gacaaaggct
     5641 tccaaaagct cggggcggcg gttgtgcgga taatgctgtt tgacccattt gaccgcatcg
     5701 gcatacagtc ccaaacgttc gtacttttcc gcaaccgata cgctcaattc cccgtttttg
     5761 aggctgtcgg gaatccgctt caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc
     5821 atctggcggc ggtatgccca attttgatac cgttccattt ccgatttgcc caacgcgccc
     5881 gccttggaaa gtttttcggt ttttgccaga acctgcaacg catcgccccg atcgaaggcg
     5941 taacgaagtt gcagacgcac gaggcgcgta aggttggcat tcatcttcgc cgccgcatga
     6001 agattggctt ccgccgcttc gtaatcgcgc cggtttaacg ccgattccgc cagcagaaga
     6061 tagcgggaaa gctgctgttt ttccggcagt ttggcgattt ccgcaagata acggtcgcgc
     6121 agctcgatat tttccatctg tcctgccgcg tgcgcgccca gcatcaatgc caaagtccgg
     6181 ttgtctccgg cctctttgtt gcccaacact cgagaggctt cgagttccgc cttttcaaaa
     6241 cgcccttcga aatacgccaa acccgcctta ttcaaggcaa gcgcggcctt gcggcctttc
     6301 cgcgccgaac cggaacgccg catattttcg gggatattga gtacgccgat gatgaattta
     6361 aacaggaaat accacaccac gacggcaatc agcgaaccta acacaaaggc gtgcaggttg
     6421 attctgagca tggtctgtcc gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc
     6481 agcgccagtc cgacggcggc ggcaaacagg acaacaatcc agactaccgt tttcatgcgc
     6541 gttctccttt agcggacggc gaacgccctg ccttttccgg tgcgggctgt ttttttgcag
     6601 gctgttccgg tttgcgttcc gaaggcaggg acggtgcttc tatggccttc acatccgatg
     6661 cggcagccgg ttcggaagct gtgttttcgt ttgccggttc ggaagccgcc tgttccgctt
     6721 cttgattttc cgccgccgtc atgcgcgtac cgtcgcgata ggcgcggacg gcatttaaac
     6781 tggtcttcaa gccgtcatcc gccgtcatgc gtacatccag tgttttcaat tccgccagtt
     6841 ctttcagcca cgactgcgtg gcgggcgact tggcatcgaa atactgtctg acggcggctt
     6901 cggcattgtt caaatcgccc tgatagactt cgccgttgcg ctgcatcaat gcggtgcgcg
     6961 catccaacag gcgcagacgc aggttttcac gcacaaaata tgcctgttcg ggagaaatca
     7021 gcatggcatc gttgttttcc aaacggcgga tttcgaccag ccccttcaat gtgccgaggg
     7081 atttttccca tacgttctgc caccatgaag cggaaacggc ttcgttgcgg gcctgtacgc
     7141 ccggtttcaa tacgccgtcc aaaatcagcg gcagtccgga tacggcggtt tccaaccggt
     7201 cgaggcgcaa tgccgtgccg gaaatatcga catagggacg gtttttcagt tccgccaaat
     7261 cgctgctgac cgcctgtttg atcggcagaa gctctgcctg atcgaaacgg gacaggcggc
     7321 tgtcgatatg ctccaacacg ccgactgccg tttggatatt gccggtcaac accagctgtt
     7381 gcgccgccag attgagtatg gtctcggttt cgtccaccag ccaatcggca cgccctttgg
     7441 tcagctcgcg ataggctttt tgcgtcatca ggatttgttc gccgttggct ttgacaccgc
     7501 tgtccaaacg gtcgagttcg gactgtatgg cactttggcg gttgaggttg tctttgagca
     7561 gggcggcgtt ttccgactcg cccaaggcgg ctttgtcgat tttctggttg aatgccagct
     7621 cttggttttt caagacattc tgtccttgga caaacaaaaa accacttgca cccaaaccca
     7681 atgccgccaa taccaaggcg caggccgcca aagcgttgct gccggactgt ttgacgataa
     7741 aaggagcggc gggaatgtgt acttctgttt cgttttcttt gcgtggggaa gaggtttcag
     7801 acggcatttc ttttgatgcc tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt
     7861 cgcccaccgt tttgctcctt attggtttga ggttccggga aagaccatgc cgtctgaaac
     7921 ggaaatggaa gaataggaaa gcgcggcttc cagcgtaggg acggtttcga ccgaacacac
     7981 gccttcgcgc ttcaatgcct ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt
     8041 gaagaatcgg gaaaattgcg gcggaagctg tgcaaacagc gactgcacaa gttctgtgga
     8101 cgtaatatag gcggcggcaa tattttcggt ttggaaattt tgaaagttca aaggtttatg
     8161 ccgtctgaaa tagacttctg ccacctccgt ccgaaaacct ttctcctgca aggcattcat
     8221 cagaaaatcc cgcccgccgt gtccgcgcac agacaatacg cgcgcacctt cgggcagact
     8281 gttccaaacc ggcaggcgca aaaccgcctc gctgtcgttg ccgtcttcag gtgcaaacac
     8341 gcccgcgccc gcataacgcg ccaaggcacg gcggctgcct tgccctacgg caatgtgcgc
     8401 ctttatgccg tctgaaaggt taaggtacgg gacggcggtt tcaacggcgg tcggactgac
     8461 ccaaaacacg gcatccgcac gggcatacat ttccggcagc cgtttcaggc ttgcttcgtc
     8521 ggtttcgatt tcaatcggac tcaacacttc cgcctgccat ccggcacgac ggcagacttc
     8581 cacatcgtcc ttcgccctgc ccgacgggcg gacaatcagc atgacgggct tcgctgtttc
     8641 catcgcctta tccgccccga ttaccgggaa aaatcgtcat cgttgaaatt cgcctgcgcg
     8701 gttttcggcg tacagcctac ccgtgcgact tccttgcccc tacgctccac aacgacaccg
     8761 ctgctttccg tatgagtcac ggaatcgaag cctgtccata cggtgtacat aaattcgccg
     8821 ttttggaatt tcatcgttgc ccggcgtccg ctgcccatgc cttgccacct gtcggaacgc
     8881 cccaacaggt cagctttgct gttgcgtatg gcaatttctt tttttgccgc actgccgaac
     8941 gaatattcgt aaagattgcg gttgattttt tgaacttcta tgtatttgtt gttgtccgtt
     9001 ttacaggaaa ataccgtatc cgcccacacg gtttgggcgg cagccgacag aagcaatgcg
     9061 gcagaaagga acattttttt catcataatt tccttaatgg ttgaaacccc gccattcgga
     9121 catccgtcct tcgtggcagc agcatcagat tttatttgaa aggggtgtaa cccctttcaa
     9181 attagggcaa cacatagggc ggcgctttat gtgtcgtcct gtgtgttgaa acatcattgc
     9241 ccgtatcctg aaatagaagg ctgtgtttta acaatccgtt gattgcttaa gatgaattca
     9301 cactaaaaaa cagttctcta tttcatctat tacggcgggt tgttttgttt ataagggtgg
     9361 taatctacaa attgttacta aatagcaagg tgtgaatgaa acggcagata taatcaaaat
     9421 cttcttgttg gtcaaaatac ggtaacataa tgctttttaa tttttcgcct ttgggtttac
     9481 gtccataaga aaacagatag cggtttgctg taatacattt agcgtaatac agttttactt
     9541 ctattggcat atcggactgt atgggtgtta atacggcgac atcactattt gctacaaatt
     9601 cacaagttga aacatagcta taagtatggc taccttcgcc attggtagaa gtgaaaagcg
     9661 tatggcatgg aaaaatatgt ttttcatcaa cgctatcacg agcgatataa cttcgtagcg
     9721 ttcttgcttg cgtactggca ggacggatat agacaaccgt gtctttttgc cgttgttcaa
     9781 attcttttaa tttggttgcg gcaatgccgt tttgaatagt aaaaatttca tcaagtcgtt
     9841 ttgattgtcg aaccacaggc aaatcaagcg gttcttttaa tttcggttcg gttacacccg
     9901 cagatggatt taaattcacg ctttcaaccc agcttgggat ctcatccaaa gatggcacaa
     9961 acagttgtcg taacgtgcgg ttagcttccc taccgcaagc agaaaaacgg taggcattgg
    10021 caaaaatagc agcacaataa aagagttttt gttgttctgt catttctgtt ttgggattta
    10081 aaacagcaac attttgagct gtataaaatg gtttgttttg taaaaaagta gataaaacag
    10141 aaccacctaa tgcaacagtt aataatcctg ctggataagg ttttgtattt ttcaataaat
    10201 ccacagatgc tgttacacca ttatttttgc ctgacctgcc tacaaagttg attgttggat
    10261 tgaagctgct cattttattc aaatctaatt ttgaaccgta actcacatca aaaatctctt
    10321 gcaattcaac cattattcat cgtctccgaa gtcgttattg tcatcgtctt catagctttc
    10381 attatccaaa ttttcagtcg gccctacttc cgctcccagt agttgaaata aagcaaattc
    10441 acgcacgact tgctcaaaat ctgctcgggt aattttggaa taatcagttt ccaaataggc
    10501 ttcggcaacc cattcatcgt tatcagttac aaatgctgtt accgattctc ctgtgtgaac
    10561 ttcgttgttt aaataatgtt ccaaccagcg cgctttaatg ccctgccagc ggtcgtataa
    10621 atcaatgcgt cccaagtgtt tggttttttc aaaaccgtca tcacgccagt agccaaacca
    10681 cgttttgcga caggcttttt tcgtgctgat ttcttcttgt gtgtccgggt caatcacgac
    10741 tgtctgaaaa tgcggtttat gggcttcaaa tacgacaatg cacgttaccg tgccaactgg
    10801 gtaaaacagt tcgctcggca tagacatcac ggcttttaag cgatgatatt tcacaatttc
    10861 gcttttggct ttgcttggtg caatcacgca actgactgga ataatggcaa tgcctgtgcc
    10921 accttctgcc aacatatcca acatttcttt gacaaaataa agttcatgca attcggcatc
    10981 gcttttggat tgggcgtaag gtggattgag aaagccaaca tttgggcgtt ttaagcctgt
    11041 ttcaggtttg ggatttttga ttaaatcctg aaaatcagtc atgaaacaac tgctttggtg
    11101 taaattggct ttaccgtctc cgcgtaaaat catattactg gcggcaagag caaacatttt
    11161 tgggttgttt tcaatgccaa tcagttgata ttttttagag atggcttacc caaattgcta
    11221 attctagcaa cagatttacc gtctaaattg ataccacaag gaacacaaac ttgttctttg
    11281 gaaatgccac gtttcgttgc tctcgttcct cgtttgtgag caggacgtgg cagattaaaa
    11341 tttttgtggt gccctttgta agaaatcgtc gaataagttt catcagcttg cacaatgccg
    11401 tctaactcaa cttcattcat catgttttga agtgcgtcca aaattttgtg ccgccaagta
    11461 aacgcagtag caagattgat tttgcaaatt tcagcacatt tgcgaagtgg gtatttttca
    11521 atcatacaat gaatgtattt ttcccaaact tcaatgtctt tttgcgtgtt gtagagaata
    11581 gtgcctgtct gctcaacaaa agacttttta caatcacgac acaaaaaacg ttggttgcca
    11641 caatctttgc catttttgac gaaatgtgta gattggcaat gtgggcactt cgtaactttt
    11701 cttggttcaa tgactttttt aacctgttct tttgagctga cagaagtcaa aaaagcctgt
    11761 ttatccgtat cggacaaatt ttcaaaaagt tgttttaaaa tgtctaattc ttttgttacg
    11821 cccgacatta taaaatccct tatagttcaa tttgttataa gggattttat caaaaattag
    11881 tgcaaaaatc aacggattgt taaaacacag ccaaatagaa aagaaaatgc ccgttttacg
    11941 gtcttctttc cgacaaaatg ccgtctgaaa cgaatttcag acggcatggt cgaaaattat
    12001 tgcgcggcaa gcggtttgag caggagggtt tcgccgcttt gcacgacatt ttccagcaaa
    12061 tcgatgttcg gatgcttccc cggatgggcg cgcgcgtcgg caacatgttc ggcaaaccat
    12121 atcaaaccct gttctgccgc ttctttaccc aattttccat caaaacgttc cgccaaggca
    12181 ttgtacagct tgagcgaacc gagcttgccc tgaacggcgg gaatgtggtg aaccgttttg
    12241 ccttcggcat cctgaacatc caaaccgccc agatggtcga ttgaaggaaa tgtggcgaga
    12301 tagtcttgga aattcatatc aaatccgaaa atcagtcagt tagcgcgtgc cgaaaatctt
    12361 gtcgcccgca tcgcccagac cgggaataat atagccgttt tcgttcaaac ggctatccag
    12421 cgcggcggta taaatcgtaa catcgggatg cgcatcatta acagccttca cgccctcagg
    12481 ggcggcaacc aagactaagg ctttgatgtt gcggcaacct ttttctttca acaggtcgat
    12541 ggtggccacc atcgaaccgc ctgtcgccag cataggatcg ataatcaaag ccggacgctc
    12601 gtccatactg tccacaaatt tctcaaaata ggaaataggc ttcagcgttt cctcgtcgcg
    12661 ctgcaaaccg accacgctga ttttggcagt cggaatcagg tcgagcacgc cgtcgagcat
    12721 acccaaaccg gcgcgcagga tggggacgac ggtcagggtt ttgcctttga tgcggtcgcc
    12781 ttcaatctgt ccgcaccatc cgtcgataag gtatttttca atttcaaaat cacggcttgc
    12841 ctcgtatgcc atcaggcgcg ccaactcggt ggtcagcgta cggaatttgt aggtgctgca
    12901 atccgcctcc ctcatcaggg tcagtttgtg gcggacgagc ggatggttga taacgttaac
    12961 gttcatcgcg gtgttccagt gtgattttga gacgcgtcat tatacttttt tttaatttgc
    13021 cggaaaagaa aatgccgtct gaaacacgtt cagacggcat tgccgaatcc atcaggcttt
    13081 cagcagctct tgcagctcgc ctgcctcata catttccatc aggatgtccg aaccgccgac
    13141 aaactcgccg ttcacataaa gttgggggat ggtcggccag tcgctgtatt ccttaatgcc
    13201 ttggcgtact tcgggatttt ccaatacgtt gacagcaacg taatcggtgc agcctgccgc
    13261 gttcaggatt tgcacggcgc gggaagagaa accgcattgc ggaaactgct tcgtaccttt
    13321 cataaacaat acgacgcggt gtgtcgttac tacttcttta atttggtcgt ggatggaagc
    13381 catcgcttat tccttttgat aaagttgaca aaatttgtca ggtagggcgc attatacgca
    13441 aaagcggaaa cttcccacaa tatttgccgt atatcgcgca gtatcaatgt tgcgaacggc
    13501 aatattccca ctcctcgaca atcctgctgt ccggcaaatg gcacagggca tatccgccgc
    13561 cgctttcgtc tttttttact tcccgtctgc cgcctttggc aatgcagaat ttggaagcgg
    13621 aatttgccat gcctgtccga aacgtgttgg caggcagccg gcagcagcgc ggacatgcat
    13681 acggatgaat tgccttcatg gttttcctta tcgtcggcac agggtcagac gacccgcata
    13741 tcgggtactt ttttaaacag ccggtaaaag ttttccgtcg tatatgccgc aacctgttcc
    13801 aatgtttgat tccgcaattt ggcaatatgt tcggcagtat gacgcacaaa agccggttcg
    13861 ttctgcctgc ctcgtttggg aacgggggca aggaacggcg catcggtttc cactaaaatg
    13921 cggtcgtccg gaacgtattt cgccgcctcc tgaaccaagg gggcgttttt aaaggtaacg
    13981 attcccgaga aagaaatata aagccccaaa tccatggcag cccgggcaaa accgacatcc
    14041 tcggaaaaac agtgaatcac acccgaattg gttttacatt cttttaaaat cgccaaggtg
    14101 tccgccgccg catcgcgcgt atggacgata acgggcagtc cggtttgatt ggctgcttcg
    14161 atgtggtctg caaagcgttt gtgctgccaa gacaaatcgc ctttgcacca gtaataatcc
    14221 aaacccgtct cgccgatgcc gaccactttc ggatgggcgg cggcttcgac catttccgca
    14281 atggaaaatt cttcggcttc cttgctgtcg ggatgtacgc ctatggtgca atagatgtgt
    14341 tcgtgcgctt cggcgacggc aaagacttcg gagaagcttt ccctactgac gctgatggcg
    14401 agtgcctgcc ccacgccgtt tgcttccatg ttggacaaaa cttcgggcag gcgttctttc
    14461 aaaccttcaa aattgaggtg gcagtgcgaa tcgatgatat gcatagcgtt acatggtaaa
    14521 cgtaggtctg ctgccgctga ttgtgctgcc cagttcgact tcgatctggt ctttgacttt
    14581 caggcagttt tcgtgttttg tgaatgccag ccccacccct ttgggtttgg aggaagtact
    14641 cgcaggatta atccaggcga ccttggtcgg caggaacagt ttggggaagt tgaggatttc
    14701 tacggcaagc agaatatcgt ccccgatgga aaatacgtcg tcggtctgca caaacagacc
    14761 gccgtgttcc aaaaacggca tataggagct gtacagcaga ttcatgtctt tcagctgcaa
    14821 cgacatcatt tttgccggaa tattttgtcc gtctgacata attcacctgt tctctttctt
    14881 caattccaaa tagttgatga gcagatgctc gatctgcatt ttgacattta aagtatgaaa
    14941 cccgtaaggg gcaagctgtt tgagcatatc ctccgccgca aatacattgc gcggacggaa
    15001 acccgatgcc gtctgaagca gcctgtcttc ataagcggga taatagacgg gtttcatgtg
    15061 ttgcaggcac aagcctaaat cgaccagcca tttctgcatc cacccgacaa atacggcgag
    15121 cggaagtttt tccttatcga aaagcgcggc gtaatccaaa atcttcaaca atctcggctc
    15181 tgccaatatc tccagcagct ttgccctcaa ttcccgaagc tcgccttcct cctgaaacag
    15241 cggcgcaccc gaatggaaag ccagacgttc ctcaggctcc gccacacccc tgtcgcgcag
    15301 atacgccaat gcttccccat gcgaaggagc gggcaaaacc ttcttccggc agcggctttt
    15361 aatggtcggt aaaaccttgt ccgccgcgtg gcttaccagc aaaaagacca cttgtggcgg
    15421 cggctcttcc aacactttca acaaactgtt ggcggcttgg acattcatac tttccgcagg
    15481 atgaatcagg atcacgcgca aaccgccccg taccgaagtc aggtacacat tatcgatgat
    15541 ttccctgacg gcatcgattt tgatccgcaa cagtttgcgt ccgttttcgg gttcgtccgc
    15601 cagaggcgtg atttcgtaaa aatcgggatg gcttccccgt ccaaacagat ggcaggacat
    15661 acattcgcca cagggtttgc agcccggtgc aggggtttcg cacaacagcg ccttcgccgc
    15721 aaagcgggca aacgcagtct tccccgtccc ctttttgcca acaaacagcc acgcattagg
    15781 acggctcgtc caatgctcgg caatctgccg ccattgttcc tgatgccacg gataaatcat
    15841 gtcaaactgc ccgtccggaa atccataccg ccatcagagc agttccaaat cggggtcgga
    15901 actttgtgcc ctgcggctgc gcaactgtac cgccaaacgc aaatcatgtg cggaatcggc
    15961 atttttcaag gcatcctgca aggaaatctc gcctttttca tacaattggt aaaggtgttg
    16021 gtcgaaggtc tgcataccca gggtagtgga ttttttcatc acttctttga tttcatggat
    16081 gttgccgttg tgaatcaact ccgaaatcag gggcgaattg agcagcacct cgactgccgc
    16141 caccctgccc ttgccgccgt ctcgcggaac gaggcgttgc gaaataaacg cctgaaggtt
    16201 gagcgacaaa tccgtcagca attgttcgcg ccgctcctcg gggaagaagt tgatgatgcg
    16261 gtcgagcgcc tgattggtgc tgttggcgtg cagcgtcgcc atacacaaat gccccgtttc
    16321 ggcaaaggcg atggcgtagt ccattgtttc acggtcgcgg atttcgccga taaggatcac
    16381 atccggcgcc tgacgcagcg tatttttcaa cgccgccatc cagttttccg tgtccacgcc
    16441 gacctcgcgc tgggtaatga tgcagttttt gtgttcgtgg acaaactcga tcggatcttc
    16501 gatggtgatg atgtgtccga acgaattttc attgcggtag tcgataagcg aggcgagcga
    16561 agtcgatttg cccgagccgg tgccgccgac aaaaataacc agcccgcgtt ttttcagcgc
    16621 aacatccttc aaggccggcg gcaggttcag gctttcaaac ttgggaatct tgctggtaat
    16681 cgcgcggaat accaacgccg tcgcaccgcg ctgtatcatc gcattgacgc ggaagcggct
    16741 ggtgtccggc aggctgatgg cgaagttgca ctcgttggtc gatgaaaatt cttccgcctg
    16801 cttcgcactc ataatcgaaa aggcgatttc catacatttt tccgccgtca gcggttcgtc
    16861 cgtgatgcgg gtgattttgc cgtccagctt catagcgggc gggaaatggg tcgtcacaaa
    16921 caggtcggaa cctttgtttt tgttcatatg gcgcagccat gcgaacagtt cttcctttgc
    16981 agtcatattt tcatcggtaa acatcctcgt cccccaatct tcaaacaatc tgtaccttcc
    17041 cattctacca aaaatccgcc gtgctgtctg aaaccgtttc agacggcacg gacaaaccgc
    17101 ctccactcaa tcctgccgtg ttagcgtgta taattcggcg ttctatccga aattcacagc
    17161 aggttagcaa tatgtctttg aaaacagtag ccgttatcgg cgcgatggaa caggaaatcg
    17221 agcttttgcg cgagatgatg gaaaatgtca aagccgtctc tttcggcaga ttttccgcct
    17281 atgaaggcga attggcggga aaacgcatgg tgcttgcatt gagcggcatc ggcaaggtca
    17341 acgcggcggt tgcaacggct tggcttatcc gtcaattcgc accggactgc gtcatcaaca
    17401 ccggcagcgc aggcggtttg ggcaagggtt tgaaagtcgg cgacgtggtg attggcacgg
    17461 aaaccgagca ccacgatgtc gatgtaaccg cattcggcta tgcccggggg caagtgccgc
    17521 aactgccggc aaggttcgct tcagacggca tattgattga aacggcaaaa cgggcggcgc
    17581 ggacgtttga aggcgcggag gtagaacaag gcctgattgt cagcggcgac cgctttgtcc
    17641 acagcagcga aggcgtggcg gaaatccgca agcacttccc cgaagtcaaa gcagtggaaa
    17701 tggaagcggc ggcgattgcc caaacctgtc atcagttgga aacgccgttc gtcatcatcc
    17761 gcgctgtttc cgattcggcg gacgaaaagg cagacatcag ctttgaagag tttttgaaaa
    17821 cggcggcggc aagttctgca aaaatggtgg cagaaatcgt caaatcttta taaaaccttt
    17881 atcaaatcat gccaaaacag cggttttccg ttagaatatc gggctatttc cacgttgcgc
    17941 ccgatattcg gacgtaccgc tgtttgttca gacggcattt ctaaaaccta ccgttcagca
    18001 ttaaaaacaa ctcaatgaaa aatatccgaa atttctccat catcgcccac atcgaccacg
    18061 gcaaatcgac gcttgccgac cgcttcatcc aatattgcgg cggtttggat ttgcgcgaaa
    18121 tgagtacgca ggtgctcgat tccatggaca tcgaaaaaga gcgcggcatc accatcaaag
    18181 cgcaaaccgc cgcactcaac tacaaagcac gcgacgggca ggtgtatcag cttaatttga
    18241 ttgacacgcc gggacacgtc gacttctctt acgaagtttc ccgttcgctg tcggcttgcg
    18301 aaggcgcgct tttggtcgtt gacgcgtcgc agggcgtgga agcgcaaacc gtggcgaact
    18361 gctataccgc gattgatttg ggcgtggaag tcgtgcccgt tttgaacaaa atcgacctgc
    18421 ccgccgccga ccccgagcgt gtggaacagg aaatcgaaga catcatcggc atcgatgccg
    18481 tcggcgcggt gcagtgttca gccaaaagcg gcatcggcgt ggaagacgtt ttggaagaaa
    18541 tcgttgccaa aattcctgcg ccgaccggcg acgaaaacgc gccgctgcaa gccgttatcg
    18601 tcgattcgtg gtttgacaat tacgtcggcg tggtcatgct gattcgagtg aaaaacggca
    18661 ccatcaaact gaaagacaaa gtgcgcttta tgagcaccaa ggcggaaacg caggtcgagc
    18721 agctgggcgt attcacaccg aaatcggttc aaaaacaaga actaaaagcc ggcgaagtgg
    18781 gttttttgat taccggcgtg aaagaattgg gacaggcgaa agtcggcgat acggttactt
    18841 tggttgccaa ccctgccact gagccgctgc ccggtttcca agaagtacaa agccaagtat
    18901 ttgcgggtct ctatcctgtg gaaagccacg attacgaagc cttgcgcgac gctttggaaa
    18961 aattgcagct gaacgatgct tcgttgaaat tcgagcctga agtttctcaa gcattgggct
    19021 tcggcttccg ctgcggcttc ttaggcctgc tgcatttaga aatcgtgcag gaacgcttgg
    19081 aacgcgagtt cgacatggat ttgattacca ccgcgccgac ggtggtttac gaagtcgtgt
    19141 tgaaaagcgg cgaaaaaatc gaagttgaaa acccgtccaa actgcccgac atcggcagca
    19201 tcgaaaccat tctcgagccg attattaccg cgaccatcct tgtgccgcag gaatacgtcg
    19261 gcaacgtcat gactttgtgt aaccaaaagc gcggcgtgca ggtcaatatg cagtatatgg
    19321 gccgccaagt gatgctgact tacgacttgc cgatgaacga agtcgtaatg gactttttcg
    19381 acaaactcaa atccacttcg cgcggttatg cttcgttgga ctaccatttc aaagaattcc
    19441 aaccgtctga tttgattaag ctcgacatta tggttaacgg cgaaaaagtc gatgccttaa
    19501 gcctgattgt acaccgtcaa agcgcggttc acaaaggccg cgagctggca tcgaaaatgc
    19561 gcgaactgat tccgcgccaa atgttcgaca tcgccgtcca agccgccatc ggcagccgga
    19621 ttatcgcccg agaaaacgtt aaagccctgc gtaaaaacgt tttggcgaaa tgttacggcg
    19681 gcgatattac gcgtaagaaa aaactgcttg aaaaacaaaa agcaggcaaa cgccgtatga
    19741 aacaagtggg caacgtggaa attccgcaaa gcgcgttctt ggcgattttg caggtgagcg
    19801 acaaataata tttcggcaca tagggcgggc gcgtcgggaa cacctctcaa accgccctga
    19861 tttcagcggc acgcgcccgc aaacaaattt tgcaatttgc cgccttaaaa cggttgcacg
    19921 gccgggcggg tttttaaccg ttaaggaaca acaatgaaca caatgctaat gtcgggcgcg
    19981 gctgccgcgc tgcttgccgg catcatcctt tatttcaaaa gcgacaagaa acggcaggaa
    20041 aacggtgaat ggagttccgg cctcgaatac gcctatatcc tgacggcggt cggtgtgttt
    20101 gccgctttgt ccctgtttat gagctttacc gccgttttcc tgattttcgt tgtattgtgc
    20161 ggtacggctt ggggggtata taaataccgc ctgaagactc atcccgaaat ctcggaaagc
    20221 agccacttcg gcgattattt cggcagtttc ttccctaccg ttttggtatt gttcctcatc
    20281 cggtcgttta ttgccgaacc gttccaaatc ccgtccagct cgatgcgccc gggattaatc
    20341 aagggcgatt tcattttggt cggcaaattt tcctacggcc tgcgcgtacc cgttttaaac
    20401 aatgtattca tccccacggg caaaatcgaa cggggcgatg tcgttgtttt taactatcct
    20461 ttgcagccgg agatgaccta catcaagcgt attgtcggca tcccgggcga cattgtcgaa
    20521 tatcgagata aagttttgac ggtaaacgga aaaccggctt ccgacattcc tgacggcaca
    20581 taccgttatc ctgacgatac cgacccatcc gaaatccaca acacggatat gttccgcagc
    20641 ggtctggacg gcaaatcctt caatattctg aaaaaagaag gacagcctgc cgtttccctg
    20701 cccgtattgg gcaaatatac ctccgatatt atgtctgaaa acggatattc catagatcaa
    20761 agcggtctga accattgcca atatgccgac gacggcagcg gtttcgtgtg caaagttccc
    20821 gaaggacgct atttcgctat gggcgacaac cgcgacaaca gtgccgattc gcgctactgg
    20881 ggctttgtgg atgacaagct ggttgtcggc aaggcaatgt tcattttgat gaacttcggc
    20941 gatttcggca gggccggcac ggcaatccgt taagccggaa aattacgccg gaacatcaaa
    21001 aatgccgtcc gaaccctgtt tcagacggca tttttatcat caaactcctt gtttccctac
    21061 cggcagcctc ggggataata tagtggatta aatctaaacc agtacagcct ggcctcgcct
    21121 tgccgtacta tttgtactgt ccgcggcttc gtcgccttgg cctgatttga atttaatcca
    21181 ctatatgcac cgccttgccg gcattatcga tatgggcggc acatatggat tgcctggaaa
    21241 aatgatgcgt tcgggcggcg tatgatttgc ctgtgtttaa atcatcggtt gattttatcc
    21301 gtcggcaagt gaaaagccgg ttatttccgt ttgtgtggaa acccatccca ttgaataaag
    21361 ggcatttcgt ccgactgacg gtgtgggcgg catatggaaa tacggacatt attttagttg
    21421 cattgccatt cctgcggagg cggctttttt gattctgccc gccccgatac cgtctgaaag
    21481 ggcttcagac ggcattttcc gctccgcgcc gttgtcggca tgtttggtct tgccggtttt
    21541 gactgcctgc cagtccaatc gtacgcaggt ttgccacggc agaaaatctg atgccgaccg
    21601 gagtcaaagc tcggacggct gtttcagcga accgaggtaa atcgccgaaa gcgtcagcac
    21661 cacccctgcc cactgcacgc cttcaatcgt tttgcccaat ccgaaataat cgatgaacag
    21721 ggcggcgacc ggttcggaca aaagcaacag ccccgtcagc gacagggaaa gcagcggaat
    21781 cgcataggca accatcgccc acgcaaaaca ctgcatcacc acgccccgta cacaagcacc
    21841 aaacccgaat ctttccaagt ggtcggataa agtgcgccgc cgtccatcag caatgccgga
    21901 acaatcggcg atgccgcgcc gcccaaactc aaaatcatca ttgaagggaa aagcgccacc
    21961 tgttcgactt catgagtctt gcggacgaac accatagata gggcaagcat cagcccggac
    22021 accaaaccgc tggcgaatcc ccaaaccgca ttaccgttgt agccgaattc cgcaccggca
    22081 atcatcgcca cgcccacaac cgccgacatc aagcttgccg cctgcagcct gcccaaacgc
    22141 tcgccgaaaa agaaaacacc gattgccgac aagaaaaaga tttgcaggct gttgagcagg
    22201 gtggaaatac ccggcccgac cgcgtgtatg ctttcgtgcc aaagtgccaa atcgaaagcg
    22261 agaaacacgc ccgccgtcag ggcatatcgg acggtttttc tgttttttgg gaatttttgt
    22321 ccgaaaaacc gtgccaaaaa ccaaaatacc aataccgaaa tcagcaaccg ccaaaatgcg
    22381 attgcatacg ggccgacggg gacggatctg acaatcaggc tgcccaatcc gaacaccgcg
    22441 caacccagca ccagcaatgg tgcggcatat ttttttccgt taccgaccac aatatcccct
    22501 cccttcccca tatggctgcc gcgccgccgg caaaagtgcc gccattccga aaatatgccg
    22561 tctgaagccc aaagccccgg ctgcgcccgg acaaacggct gactttacgg gcaggccggc
    22621 tattttccgt tggggcaaaa tccgtcctgc cggataaaac ccgaataaaa aatatttccc
    22681 tcaagcaatg cggcggcgcc aacacctgat taaaacacaa ccggcgtttt cggtatatat
    22741 gcaaaaacgc attttccggc acggcgttac accgataccg aaaaatgcgt ctgaatgttt
    22801 cgacaatccg attaaagcat tatgccaaat ctgcaaaaag tggcgtagag agatagcgtt
    22861 cgccataaga aggcagcagc acgactatca gcttgccttc gttttcaggc tgtttggcaa
    22921 gctgcaacgc gctccaaacc gccgcaccgg aagaaatgcc cgccaaaatg ccttcttttt
    22981 ccgccattgc acgggcggtt tcaaaagccg cttcgttcgg cactttggta atgctgtcgt
    23041 agatttcggt attcaaaacg gtcggaataa aacctgcgcc gataccttga atcgggtgcg
    23101 ggcctttttc gccgccgctc aatacggggg aagcctcagg ctcgacggca accactttaa
    23161 cttcgggttt gtattttttc aatacttcgc ccacgccggt aatcgtaccg cccgtgccga
    23221 cgccggcaac gaagacatcg actttaccgt cggtatcgtt ccaaatttcc tcggcggttg
    23281 ttttgcggtg gacttcggga tttgcctcat tgtcgaactg gcgcggcata aaataagtgt
    23341 ctggatgagc gtccaccaag gattgcgctt tggcaatcgc gcccgccata ccttcggcgg
    23401 cgggggttag aatcagttcc gcgccaaacg tgcgcaacag cattttgcgc tctttgctca
    23461 tgctttccgg catggtaatc gccagtttgt agccgcgtgc ggcacatacc attgccaaac
    23521 cgatacccgt attgccgctg gttgcttcga caatgacggt gtttttgttg atttttcccg
    23581 ctttttcggc ggcctcgatc attgcttcgg caatgcggtc tttgacgctg ctgcccggat
    23641 taaaaaattc cagtttcacg gcaacctctg ccttcaaacc tttggtcaaa cggttcagtt
    23701 tgaccaaagg cgtgttgccg atcaattcgg tgatgctgtt tgcaattttc atcttctgct
    23761 cctgaataat aaggtttcga cagtaaggcg caatccccat cggtatcctg tccgattgca
    23821 ggggatttgc gccgaaccat aaacaaattt cggatatggc tttatcctaa acctatgccg
    23881 cctaaaaaac caatacccaa aatttatttc tttataactt tttgttccga tacagacggc
    23941 atcacaatca tctgccgatt tcggcaagca gcatcgccgt taccgcaacc tgcatcaaca
    24001 tcgccgccaa cacgccgagt cgcagctgcg aaccgccgaa cgttgacggc ggctgccggt
    24061 ctataccgca gcggtggaca taggcaaccg ccatcaatac aaactgcaac acgaaccaat
    24121 aaaccggctt cagcgcataa agtttgagca atgccgcaaa cacaaacact tgaaccgttc
    24181 ccgaaaagca ataatgcacc aacgcacata ttccgacaaa agcaagcgtc atcgatacat
    24241 tactgacggc aaaaagcccg agcagcggat gttcggggtc tgcctgccat ccgtttccat
    24301 ctgttttccg gttccaatac ccgatgaaaa aaaatatgct gcccaaagtc aggtaaaaat
    24361 gggggatgaa caaaggcgcg gcgcgggtca ttccccacat ccccggcatc agctttaccc
    24421 ctaaaaccga gatgccgagc cacaacgcaa tactcgccca aaaccattgc aacattcctg
    24481 cgcggaatat cagaaacaga aaaaccgccg catacagtaa ggcagacaaa aaatccaaat
    24541 gcagcaaaat catgaatacg ccaactcgcc ttcaaacacg gtttccgcag ggccggtcat
    24601 catcacatcg ccgccgcagg cccattcgat atataaagtc ccgcccggca aaaccacctc
    24661 taccgttttc ccttcatcca acagccccag acggataccc gccaccacag ccgcacacgc
    24721 gcccgtaccg cacgcttggg tttcgcccac gccgcgctcg aacacgcgca aacggattgc
    24781 cgtccgtccg acaacctgca taaagccgac gttgacgcgt tcgggaaact gcctgtgcgg
    24841 ttcgatacgc gaaccggttt cgcgcaccgg cgcgcattcc acgtcatcga ctacaatcac
    24901 agcatgagga ttgcccatat tgacgcagct gacaggctga atgccggatt cgagatgcac
    24961 cccgtaaata caggcatcat ccccctcgcc cgattcgggg acaaacggta tttcagacgg
    25021 cataaacttc ggtttgccca tattgaccgt aaccataccg ttatcggaca atttcggaaa
    25081 aataatgccc ttggccgttt caacacaaat ttctttctta tcggtcaaac ccttgtctgc
    25141 aacaaaacgg gcaaaacaac gcgctccgtt gccgcattgc ccgacctcgc taccgtcggc
    25201 attgaaaata cggtaacgga aatccacgcc ttcggtttcc gaacacccga ccaccaaaag
    25261 ctggtcgaag cccacgcccc ggaagcggtc cgcccatgcc gcaatcggca cgtcctcggg
    25321 ggtaaaatcc tgactgaccg cgtcaatcac cataaaatcg ttgcccaaac cgtgcatttt
    25381 ggtaaatttc aaagttttca tattgttccg aaatcccgta ttgtctgttg cttccgaatc
    25441 cgaaatcccc gagcccgaag ggaaatgctt ccatctcccg ccgcctgagg attaaaatga
    25501 gccattatac tgtttgttac cccttacaag aaacacaatg tcccgcctgt tcctgctgct
    25561 tttcccttgg gcaacccttg ccgcctgcac ccgaaacata ccggcaagcc ccgccgtttc
    25621 aaattgcgcg catcttcaca accgcaccgt cctgtatttt gacgcacacg gcaaacccgc
    25681 cgacagcaca gcaaagggcg gattttacag gaccgtcgaa attcatgaca caaaccgcat
    25741 ccttattcaa gactttaaca gcacggggaa tgccctcgtc aaaccctatt ggtcggactg
    25801 ccggaaattg tccgttttcc ccgacaaaca tctgaccatg agccatccat gaaccacgac
    25861 atcactttcc tcaccctgtt cctgctcggt ttcttcggcg gaactcactg catcggtatg
    25921 tgcggcggat taagcagcgc gtttgcgctc caactccccc cgcatatcaa ccgcttttgg
    25981 ctgattctgc tgcttaacac aggacggata agcagctata cggcaatcgg cctgatgctc
    26041 ggattaatcg gacaactcgg catttcactc gaccaaaccc gcgtcctgca aaatatttta
    26101 tacacagcct ccaacctcct gctgctcttt ttaggcttat acttgagcgg tatttcttcc
    26161 ttggcggcaa aaatcgagaa aatcggcaaa ccgatatggc gcaacctgaa cccgatactc
    26221 aaccggctgc tgcccataaa atccataccc gcctgccttg ctgtcggaat attatggggc
    26281 tggctgccgt gcggactggt ttacagcgca tcactttacg cgctgggaag cggtagtgcg
    26341 acaaccggcg gactgtatat gcttgccttt gcactgggta cgctgcccaa tcttttggca
    26401 atcggcattt tttccctgca actgaaaaaa atcatgcaaa accgatatat ccgcctgtgt
    26461 acaggattat ccgtatcatt atgggcatta tggaagcttg ccgtcctgtg gctgtaagcc
    26521 ccattcattc ccgatacccg taaaaaatac cgtctgaaac ctgttcagac ggtattttca
    26581 ccctaagcgt tactcggcgg cattttcttc acggacaggc gcatccagca gggctttgat
    26641 ggacagacgg acacgacctc tgtcatccac ttccaatgct ttcacgttca ccacctgacc
    26701 gacttgcagg taatcgccga cattgcgtac gcgctcgtgt gcgatttggc tgatgtgtac
    26761 caaaccgtct ttgcccggca tcacgctgac aatcgcgccg acgttgttgt cgaggatttt
    26821 aaccacagta ccttcgtaca ctttgcccac ttccacttcg gcagtgattt cttcgatgcg
    26881 ttttttcgct gcatcgcccg cttcttgagt agtcgcagcg atagtgatgg taccgtcttc
    26941 agcgatattg atttcagtac cggtttcagc ggtaatcgaa cgaatggttt cgccgccttt
    27001 accaatcaca tcacggattt tgtcttggct gattttcatg gtgaacaaac gcggcgcgtg
    27061 tgcggacagc tcttgcggac ccgcaacggc agctttcatc tgatccaaga tgtgcaaacg
    27121 cgcttctttg gcctgtgcca aagcaatttg cataatctct ttggtaatgc cttggatttt
    27181 gatgtccatt tgcagcgcgg taacgccttc ggtcgtaccg gccactttaa agtccatatc
    27241 gcccaagtgg tcttcgtcgc ccaaaatgtc ggtcaggacg gcaaacttat tgccttccaa
    27301 aatcagaccc atcgcgatac cggcaacgtg tgctttcaaa ggcacgccgg cagacagcag
    27361 gctcaaacag ccgccgcaga cagaagccat agaggaagag ccgttggatt cggtaatttc
    27421 ggagaccacg cgcatggtgt agctgaaatc ttcaggtttc ggcaatacgg ccaacaacgc
    27481 acgtttagct aaacggccgt gaccgatttc acggcgtttc ggcgcgccca tgcggcccac
    27541 ttcgccggta gagtacggcg gaaagttgta gtgcagcata aagcggtcgg tatattcgcc
    27601 ggacaacgcg tcgatgattt gttcgtcgcg cgaagtaccc aaagtcgcta cggcaagggc
    27661 ttgggtttcg ccacgggtaa acagtgcgga accgtgcgtg cgcggcaata cgccggtttg
    27721 gatgttcagc ggacggacgg tgcgggtgtc gcggccgtcg atgcgcggtt gtccgtccaa
    27781 aatttggctg cggacgacat cggcttccaa gcgtttgaaa atacctttga tttcgttggc
    27841 tgccagcgtg tcggtttctt cggtaatcaa ggcttctttt accgcattcc aagcctcgtc
    27901 caatttggcg gaacgcgctt gtttttgacg gattttgaac gcttctttaa tggtttcgcc
    27961 ggcaatcccg cgtactttgg caaccagttc ttcattggtt tcaggtgctt tccaatccca
    28021 aacttccgga ttcacttcgt cggcaaattc attgattgca ttgatggcaa cctgcatttg
    28081 atcgtggccg taaaccaccg cgcccaacat cacgtcttcg ggcaggattt tggcttcgga
    28141 ttccaccatc aacacggctt tggaagtacc ggcgaccacc aagtccaatt gtgatttcgc
    28201 caactcggct ttagtcgggt tcaaaacgta cacgccgttt atataaccca cgcgcgccgc
    28261 gccaatcggg ccggcgaacg gtacgccgct caacaccagc gcggcggacg cgcccaacat
    28321 tgcaggaatg tcggaatcga tttcgggatc gacggacaca accatcgcta cgatttggat
    28381 gtcgtggtag aaaccttcgg ggaacagcgg acgaatcggg cggtcgatca gacggctggt
    28441 caggatttct ttttcgcttt gtttgccttc gcgtttgaag aagccgccgg ggattttgcc
    28501 tgcggcataa gtgcgttcca aataatcgac ggccaacggg aagaagtcct gaccttcttt
    28561 gacctctttg ttggtggtaa cggcaacaaa aacaacagtg tcgcccatcg atactttaac
    28621 ggcagcggca gcttggcggg caatttcgcc ggtttccaaa gtaacggttt gattaccgta
    28681 ttggaaggtc ttaacgtatt tattgaacat cattgttcct ttcaaaatac cgcactgcta
    28741 aaacactaat aatgcacact aaaatccgaa tgtgcatagt tagggtttca gaccgtgcgg
    28801 caggttgtaa acaagctttc agacggcatt tcggacgctg aaagcaattg cggattataa
    28861 aggcaaccat ccttaaaatc cagtattgat acaataaaaa ggccgtctga aacatatcct
    28921 tttcagacgg ccttcagtct tcaaaatcaa tcttgtgtca gtaaagtcaa tgcttcctga
    28981 tatttctcga ccgttttccg aattacatcg gcaggcactt taggcgcagg ggcttttttg
    29041 ttccaaccgc tttgttccag ccagtcgcgg acgaattgtt tgtcaaaaga cggcggattg
    29101 atgccgactt catattgatc cgccggccag aagcggctcg aatcgggagt caatacctca
    29161 tccatcagcg tcagcgtacc ttcttcatcc aaaccgaatt caaatttggt atcgcaaatg
    29221 atgatgccgc gcgatttggc atattccgcc gcttcggtgt aaagccgaac cgctttgtcg
    29281 cgcacttctt ccgccaattc tttaccgata atgcgtccgc attcttcaaa gctgatgttt
    29341 tcatcgtgat cgccgactgc ggctttagtt gagggcgtaa aaatcacttc gggtagttgt
    29401 tgcgcttccc gcataccttc gggcagccga ataccgcaaa ccgagccggt tttttgataa
    29461 tctttccaac cgctgcctgc cagataaccg cgcacaatgg cctccacttt caccggagtg
    29521 agttttttag ctacaacggc gcgtttttcc aaagctttgg cttcgttttc aggcaaaaca
    29581 tcgtacacgg tatcgccggt gaaatggttg ggcataatat gcgccagttt tttaaaccaa
    29641 aaattggaaa tctgcgtcag gatttcgccc ttacccggaa ccggggcgtc caaaatcaca
    29701 tcaaacgcgg acaggcggtc ggaagcgacc atcagcatac gtttatcgtc gatttcatat
    29761 aaatcgcgca cttttccgga ataaatcttt accaaaccaa tctcactcat ttcgcacccc
    29821 cctgaaaata tcttgaaaat accgacccga caggtttgaa tcgcaaaccg atattctagc
    29881 cgaagtcggc gcaaaacaat acccatggca caaaaagcca acccgtcaac cgtcggcaaa
    29941 gttttggcac tataataccg acagcaagtc ctacaataca ctttcaccaa aggaaatacc
    30001 tcatgagaat cctattgaca ggctcgaaaa gccaactggc acactgcctg cgcgaccgtc
    30061 ttcctgaaga ctgggaaacc attgcgacgg attccgcatc cctagacatt accgatgccg
    30121 atgccgtctg caacatggtc aaaagtttcc aacccgacgc tattgtcaac acggctgcct
    30181 atactgccgt cgacaaagcg gaaggcgatg cggcagcggc atttgccgtc aatgcttccg
    30241 ccgtttacaa ccttgccttg gcagcacatc gcgcccatgc ccgattcatc cacatctcaa
    30301 ccgactatgt ctttgacgga aaagggaaaa taccccatca ggaaagcgac tttaccaacc
    30361 cttccaatgt atacggacaa tccaaaaccg ccggcgaact gctcgcactg tctgccaatc
    30421 ccgacagcct tatcctgcgg acttcttggc tgtttagcga atacggggac aactttgtcc
    30481 gcacgatgct gaaccttgcg cgggaacgtt ccccgctgtc cgccgtccac aaccaaatcg
    30541 gctgcccgac ctatgccggc gacttgtccg ccgccatcat ccgcctgttg cagcagtcca
    30601 atcccgttcg cggcatttac cactacgccg gcggcaaatc cgtatcctgg tacgaatttg
    30661 cccggcacat ctttcagacg gcattgcaac aggatgcctc ttttcccgtt cccgaactga
    30721 aagccgtttc ggatgaaggc tctgcggctg ccgcccccag gcccgcatac agcattttgg
    30781 actgccgcaa aatcgaaaac gacttcggca tcaaaccgtc agactggcaa aaagcccttg
    30841 cacaggtcgt ttccaagctg ctctgatgcc gcccgcccct ccgtttcctc cgccaagcac
    30901 cgccttggcg gttttcttat aaaatctact gattacacaa acacatatca tctttacaca
    30961 atcatgcttc catcaacagc aaaacatgat atgattgcca acaatgacat ctcacaataa
    31021 attttctaat ttttattgaa aaaatcaata aattaagaat tctcccaacc gacaaataaa
    31081 ataaagaaag ggtcaatatg caacaccgta gaagattggc aatttaccag gcatccaaac
    31141 gtgcttcctt taccggcagg tcatcagccc cacaaaaacg taaagaacgt tgatttgaaa
    31201 aaatgccgtc tgaagtcctg cttcagacgg cattttttac cgttcgagaa actgtttcaa
    31261 cctgtcctca tccaaaaacc agtggcaaat ttcctcatct cccgccaaca aaacgggaat
    31321 cagctcattg tatttttctt ccaaaacagg attttcatcc acatcgacca cttccagccc
    31381 gaacccgtat tcatcctgaa aaggtttgag tgcatcgcgc attttgtggc acaagctgca
    31441 atattcacga aacatcaagg tcaatttcat ccgcgtttcc ttatctgtca atttgcacac
    31501 gccaaagcct tagacgcagc agaatcatgg tctatttggg aaaaaacaat gtcttcgagg
    31561 aagatgatac tcaagtcctg ccaaaaacag caaaaatgcc gtctgaacag ttcagacggc
    31621 atttccaaaa ccgttttacg cttgaacgtt gatacccgcc gtaaccgtcg gttgcgcgtt
    31681 gtgcgccgtc agaaacgaag cggcaagctc cccgcagcag ccgcagcagc tcgccacgcc
    31741 caattgcgac tgcaaatcgc ccattgtggt cgcgccggca gcgatggttt ccttgatttg
    31801 atggtcggta acggcattgc agatgcagac aaacattttg tgctccgtgt gttctcaaac
    31861 tatcgtaccg atagcggctt ttattatcgt atgcaaatat aaataaaaac ggttcgcatt
    31921 gcaaggtcgg catacacggt ttgtcttggt aattttttat caagtttgca ttttcgattg
    31981 atttcttatt aaaccgaagc agaagcctca gtttcgggaa tggtgttcct taaccacgct
    32041 atgcagccgc acattggcaa catgggtata aatctgagtc gtgttcaaat cggcgtgtcc
    32101 caacatatcc tgaactacgc gcaaatccaa tccgtgccgc accagatgcg tggcaaaggc
    32161 gtggcgcagg ctgtgcgggc tgatgtgccc gatgcctgcc ctgccggcat attctttgac
    32221 aatcatccac gccaactgcc gggaaatgcc cgtttttttc tgactgacaa acaatgcgtc
    32281 gcaactcctg cctttcagca gaagcgggcg tgcctccgta taatagcgtt ccacccaata
    32341 cgctgactcc tgtcccatcg ggaccatcct ctgcttgtcg cccttgccca gcgtggtaat
    32401 acaacccctg tccaaatcca cattgccgaa attcagcccg accgcctcgc tgacgcgcaa
    32461 gccggtcgcg tacatcaatt cgagcagggc cttatcccgc aaaccgtgcg gcgtgtcggt
    32521 atccggtgcg gcaagcagtc gggaaatctg ctgttctgta atcagtgtcg ggatgtttct
    32581 gtcgattttt ggcggtttca gcaggcgggt gggattgtcc gccctcatcc cttcccgttc
    32641 catccacaca tacaggcgtt tgcacgccga cagtgcgcgt gcctgcgaac tccgttgctc
    32701 cccgtcaaca taaaccgccg ccgccaaatc tgcttcgtcc gcatccttca gcgttctgcc
    32761 gcacaaagac agccggcggg cgatcttttc caaatcccgc cggtagctgt ccaaagtatt
    32821 ccgactgagc cgccgatcca accacaaagt ttccagcagc ctgtcaatca aaccgttttc
    32881 cataacgttc caaaccaatg ccgtctgaat cttcttcaga cggcatggtt cacatcatcg
    32941 ggaaagcgtt tccaacactt cctgtgcatg acccgccact ttgactttcc gccactcccg
    33001 gacaatttcg ccgtccttgt tcagaacgaa cgtactgcgc tcgatgccta acgactcttt
    33061 cccatacagt tttttcaatt tgatgacatc aaacaggcgg cacaccgttt catccttgtc
    33121 gctcaacagc tcgaaccgga aaccctgctt ggcgcaaaaa ttctgatgcg cctttacgcc
    33181 gtcgcgggaa ataccgacca cggtataacc caatgcctca aactgttcca aacgcgcatt
    33241 aaaatccaag ccttccgtcg tacagcccgg cgtactgtct ttcggataaa aatacacgac
    33301 caaaggcaga tgttctgccg aatgaaaatc cgcaccgctg ctcgaaggca gggtaaattc
    33361 atatttcata tccatagtcc tactcccgat attcccatta ttcaaaacgg cacgcagacg
    33421 caccgccgca attgccaaac caaccccgat tctaccgccc caaaggacaa ggattcaacc
    33481 gccggaaaca tccaaaccga cacacgacgg catgaaaaat atccatgtca aaccacaaaa
    33541 tatgttccga tttaaaaaca gaatgttata aaacccaact ccaaaaacac aacaagaccg
    33601 cccgcctacg ggcagtctcc tgtcagacga catactttac agatggctgt tttttcaaca
    33661 aaataacgcc aatactcaaa aatacggaac caaaaatgtc catccgcacc ctgaaacgcc
    33721 tgccctcatc gctgctgctc ggcctctgcc tttccctgcc gtcagcccac ctttttgccg
    33781 acaacgacat tttagggcaa tttttagaac agaacatgct tacctcctcc gatccgatag
    33841 aaatattcgc cgaaagcacg atatatcccg ccgataccca ggccattacg ggcgggctga
    33901 ttctgtcctc ccaatccgca ctggtcgtca acaacaaaac agggcaaata ctgtatcaga
    33961 aaaacgccga caggattatg cccattgcct ccatttccaa gctcatgagc gcgatggtcg
    34021 ttttggatgc aaacttggac atgaacgaaa ccgttaccat cacacccgac gaaatcgacc
    34081 gcctcaaagg gaccggcagc cgtcttgcca taggtacggc acttacacgt aaagaactgc
    34141 tgcaccttag cctgatgagc agcgaaaacc gcgccaccca tgcattgggc agaacctacc
    34201 ccggcggcat gggcgcattt gtcgccgcca tgaaccgcaa agcccaaagc ctgggcatgt
    34261 acggcagccg cttttacgaa ccgaccggac tcaacttcca aaacgtttcc accgccaaag
    34321 acctgagcct catggtcaac gccgccgccc aatatccgca aatccgcacc aactcgactt
    34381 ccaactacgc ctcggtacaa accaaaaacg ggcagcaaaa ctataaaaac tccaatgccc
    34441 tggtcaggga aggcatgtgg aacatcgaat tgcagaaaac cggctacatc cgcgaggcag
    34501 gcaggtctat ggttgtcaaa gccaacattc aaaaccaacc cgttaccatc gtattgctga
    34561 actcgcccac atccgccaca cgcgtcaacg acgcccgcaa aatcgaatcg tggatgctgc
    34621 agcaacgctc ctgacataca aatgcccggc ggaaaaccgc ccaaacacaa aaaaattggt
    34681 aatttttcct attccaggct acaattcgac acgcacaggg catctgcccg atttcacaat
    34741 cataatagcg gagttaaaaa atatgacagc taaaggacaa atgttgcaag atcccttcct
    34801 gaacgcattg cgtaaagagc atgttccggt ttcgatttac ttagttaacg gtatcaaatt
    34861 gcaaggtcag gtcgaatctt tcgaccaata cgtcgttctt ctgagaaaca cttccgtaac
    34921 ccaaatggtt tacaaacacg ccatttccac catcgtaccg gctcgctccg tcaacctcca
    34981 acatgaaaac aaaccccaag ccgcaccggc ttcgaccctt gtccaagtgg aaaccgtcca
    35041 gcagcctgcc gaataatccg cacgaagcat gacgtgtcat atctttcaat accttaccgg
    35101 acaacggtaa ggtattttta ttttcaggcg gcatttaaaa atattattga aaaacatcct
    35161 tccattcgcc cattgccttt tgcgggaggc acttcccgaa ggcggcaatg cgctggacgg
    35221 caccgccggc aacggacacg acaccctttt cctcgcacaa accgcaggca acagggggaa
    35281 agtgtgggca ttcgacatcc agccgcaagc cctgaacaac acccgatgcc gtctgcagga
    35341 agcaggttac agcaatgtac ggctcatctt ggacggacat gaaaacctga agcaatatat
    35401 tccggagccg ctggatgcag ccgttttcaa tttcggctgg ttgcccggag gcgacaagaa
    35461 tctgaccacc gacaaggaaa ccagcattcc cgccctttga gcaaccctct ccctgctgaa
    35521 agaagacggt atgcttattg ccgtcctcta tccgggacac gaaaacggca aacaggaggc
    35581 agaagccgtc ggacaatggg caaaaaacct gcctcaagaa cagtttgccg ttttacgtta
    35641 cggctttacc aaccggaaga atagcccacc ctatcttttg gcatttgaaa aactgcgtca
    35701 aaaataactg tttgcggtaa aataagccga ttctaaccct tattttgata cgagccgacc
    35761 atgcagtatc tgattgtcaa atacagccat caaatcttcg ttaccatcac cattttggta
    35821 ttcaacatcc gttttttcct actttggaaa aatccagaaa agcccttggt cggcttttgg
    35881 aaagcactgc cccacctcaa cgacacgatg ctgctgttta cgggattgtg gctgatgaag
    35941 attacccatt tctccccgtt caacgcgcct tggctcggca caaaaatcct gctcctgttc
    36001 gcctacatcg cactgggcat ggtaatgatg cgcgcccgtc cgcgttcgac caagttctac
    36061 accgtttacc tgctcgctat gtgttgcatc gcctgcatcg tttaccttgc caaaaccaaa
    36121 gtcctgccat tctgaaacac tgccatgaac aacagacatt ttgccgtcat cgccttgggc
    36181 agcaaccttg acaaccccgc acaacaaata cgcggcgcat tagacgcgct ctcgtcccat
    36241 cctgacatcc ggcttgaaca ggcttcctca ctgtatatga ccgcacccgt cggttacgac
    36301 aatcagcccg atttcatcaa tgccgtctgc accgtttcca ccaccttgga cggcattgcc
    36361 ctgcttgccg aactcaaccg tatcgaagcc gatttcggac gcgaacgcag tttccgcaat
    36421 gcaccgcgca cattggattt ggacattatc gactttgacg gcatctccag cgacgacccc
    36481 cgccttaccc tgccgcatcc gcgcgcgcac gaacgcagtt tcgtcatacg ccctttggca
    36541 gaaatcctcc ctgattttat tttgggaaaa tacggaaagg ttgtcgaatt gtcaaaacgg
    36601 ctgggcaatc aaggcatccg tcttttaccg gacaggtaat tccgcacgcg gatgccgtcc
    36661 gaacaccggt ttcagacggc atttttcctt tgccgccaac acgcgtgcaa aaaaaatcgc
    36721 cccttgagaa aggggcgcaa aaggaacaca aaccactacc aaaactttaa atctgcaaca
    36781 ctgcctgccg catactgtac ccgacaggat ataaggccct cactaaatcg tttcgagaaa
    36841 tccaaacttc ttcatcaccg acagaaaatc tgcctttctc cggcaccagc tccaacaaaa
    36901 acggctgaac cgccgtatgc agcttgtcct tacaccgccc cagctttctg gacaacgcgg
    36961 acagggcgcg tttgtaggca ttatccttgc ggtcgagctc cctcgcacta ccgacccagc
    37021 tcaaacgaag gttgcggctt tcatcttcaa tcaacaaaat acctgtttca acttccccca
    37081 cctcgggttc gcacgaaagc gcaatataat atttgccgtc aatattgaca tatgccgctt
    37141 catgcacctg acagttgcag ccggaagtgg caagcaacga ataaacagaa tcacggtgca
    37201 gcctcaaaat atcggaggaa atctgtttga aaatactttc cggcattttt ccctcctttc
    37261 gtctgctgcg ttgtttactg gattgactgc attatacaga catctcccaa
//
LOCUS       DAWTHD010000005        35927 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWTHD010000005 DAWTHD010000000
VERSION     DAWTHD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35927
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.005"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            79..1872
                     /locus_tag="WM704_000180"
     CDS             79..1872
                     /locus_tag="WM704_000180"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFA9339701.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            complement(1931..2037)
                     /locus_tag="WM704_000181"
                     /pseudo
     CDS             complement(1931..2037)
                     /locus_tag="WM704_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2071..2733
                     /locus_tag="WM704_000182"
     CDS             2071..2733
                     /locus_tag="WM704_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFA9339702.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(2818..3669)
                     /locus_tag="WM704_000183"
     CDS             complement(2818..3669)
                     /locus_tag="WM704_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFA9339703.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3743..4873)
                     /locus_tag="WM704_000184"
     CDS             complement(3743..4873)
                     /locus_tag="WM704_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HFA9339704.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRSDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            5043..5939
                     /gene="argB"
                     /locus_tag="WM704_000185"
     CDS             5043..5939
                     /gene="argB"
                     /locus_tag="WM704_000185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFA9339705.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            complement(6530..7198)
                     /locus_tag="WM704_000186"
     CDS             complement(6530..7198)
                     /locus_tag="WM704_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9339706.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(7195..7863)
                     /gene="hda"
                     /locus_tag="WM704_000187"
     CDS             complement(7195..7863)
                     /gene="hda"
                     /locus_tag="WM704_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFA9339707.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(7967..9733)
                     /locus_tag="WM704_000188"
                     /pseudo
     CDS             complement(7967..9733)
                     /locus_tag="WM704_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10126..10773)
                     /locus_tag="WM704_000189"
     CDS             complement(10126..10773)
                     /locus_tag="WM704_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFA9339708.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            complement(10770..11136)
                     /locus_tag="WM704_000190"
                     /pseudo
     CDS             complement(10770..11136)
                     /locus_tag="WM704_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            complement(11146..11817)
                     /locus_tag="WM704_000191"
     CDS             complement(11146..11817)
                     /locus_tag="WM704_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFA9339709.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            complement(11983..12702)
                     /locus_tag="WM704_000192"
     CDS             complement(11983..12702)
                     /locus_tag="WM704_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9339710.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGSF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRMKSHPGEAGSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(12784..14097)
                     /locus_tag="WM704_000193"
     CDS             complement(12784..14097)
                     /locus_tag="WM704_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFA9339711.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            complement(14172..15044)
                     /gene="hpnD"
                     /locus_tag="WM704_000194"
     CDS             complement(14172..15044)
                     /gene="hpnD"
                     /locus_tag="WM704_000194"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFA9339712.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            15215..17077
                     /gene="hscA"
                     /locus_tag="WM704_000195"
     CDS             15215..17077
                     /gene="hscA"
                     /locus_tag="WM704_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFA9339713.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            17375..17911
                     /locus_tag="WM704_000196"
     CDS             17375..17911
                     /locus_tag="WM704_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9339714.1"
                     /translation="MRTLPGWNASSPSKPTITKKKNLLHEIFSKSRIGDTELFAVDEN
                     LVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPY
                     ALPRLLADAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYS
                     EYLELFKSESDAGGRKPR"
     gene            17947..18708
                     /locus_tag="WM704_000197"
     CDS             17947..18708
                     /locus_tag="WM704_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFA9339715.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            18764..19105
                     /gene="fdx"
                     /locus_tag="WM704_000198"
     CDS             18764..19105
                     /gene="fdx"
                     /locus_tag="WM704_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFA9339716.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(19165..19705)
                     /locus_tag="WM704_000199"
                     /pseudo
     CDS             complement(19165..19705)
                     /locus_tag="WM704_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            19918..20115
                     /gene="iscX"
                     /locus_tag="WM704_000200"
     CDS             19918..20115
                     /gene="iscX"
                     /locus_tag="WM704_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFA9339717.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(20183..20584)
                     /locus_tag="WM704_000201"
     CDS             complement(20183..20584)
                     /locus_tag="WM704_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFA9339718.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            20796..21755
                     /locus_tag="WM704_000202"
     CDS             20796..21755
                     /locus_tag="WM704_000202"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFA9339719.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            21823..23133
                     /gene="tilS"
                     /locus_tag="WM704_000203"
     CDS             21823..23133
                     /gene="tilS"
                     /locus_tag="WM704_000203"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFA9339720.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            23207..23992
                     /locus_tag="WM704_000204"
     CDS             23207..23992
                     /locus_tag="WM704_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFA9339721.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADDILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYLQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            24002..24199
                     /locus_tag="WM704_000205"
     CDS             24002..24199
                     /locus_tag="WM704_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339722.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            24461..24814
                     /locus_tag="WM704_000206"
     CDS             24461..24814
                     /locus_tag="WM704_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339723.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKYRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEKQ"
     gene            24826..25131
                     /locus_tag="WM704_000207"
     CDS             24826..25131
                     /locus_tag="WM704_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9339724.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            25193..26569
                     /gene="mpl"
                     /locus_tag="WM704_000208"
     CDS             25193..26569
                     /gene="mpl"
                     /locus_tag="WM704_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFA9339725.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            26776..27828
                     /gene="bioB"
                     /locus_tag="WM704_000209"
     CDS             26776..27828
                     /gene="bioB"
                     /locus_tag="WM704_000209"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFA9339726.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(27924..28724)
                     /locus_tag="WM704_000210"
     CDS             complement(27924..28724)
                     /locus_tag="WM704_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFA9339727.1"
                     /translation="MTKAVRRKNVQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHS
                     NGFGAQGITATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDY
                     AKFKKLIRKTKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDS
                     GADVVFGQGAHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNI
                     IPITQVGDKIPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(28735..29037)
                     /locus_tag="WM704_000211"
     CDS             complement(28735..29037)
                     /locus_tag="WM704_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339728.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            complement(29079..30938)
                     /gene="ilvD"
                     /locus_tag="WM704_000212"
     CDS             complement(29079..30938)
                     /gene="ilvD"
                     /locus_tag="WM704_000212"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFA9339729.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(31111..32876)
                     /gene="cysI"
                     /locus_tag="WM704_000213"
                     /pseudo
     CDS             complement(31111..32876)
                     /gene="cysI"
                     /locus_tag="WM704_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            complement(32903..33937)
                     /locus_tag="WM704_000214"
     CDS             complement(32903..33937)
                     /locus_tag="WM704_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HFA9339730.1"
                     /translation="MRHIEIDLSGSDLHYLPGDALGVWFDNDPALVGEILDLLGINPA
                     TEIQAGGKTLPVASALLSHFELTQNTPAFVKGYATFADNDELDRIAADNAVLQGFVQS
                     TPIAGVLHRFPAKLTAEQFAGLLRPLAPRLYSISSSQAEAGDEVHLTVGAVRFEHEGR
                     ARAGGASGFFADRLEEDGTVRVFAERNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQ
                     RAAENAEGRNWLIFGNPHFAADFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDK
                     IREQAEGLWQWLQEGAHIYVCGDAAKMAKEVEAALLDVIIGAGHSDEDGAEGYLDMLR
                     EEKRYQRDVY"
     gene            complement(33942..34625)
                     /locus_tag="WM704_000215"
     CDS             complement(33942..34625)
                     /locus_tag="WM704_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFA9339731.1"
                     /translation="MSGLSGYAWAKAGNGASAGLPALQTALPAAEPFSVTVLSASQTG
                     NAKSVADKAADSLKAAGIQVRRAELKGYKAKNIAGERRLLLVTSTQGEGEPPEEAVVL
                     HKLLNCKKAPKLDKLQFAVLGLGDSSYPNFCRAGKDFDKRFEELGAKRLLERVDADLD
                     FAAAADGWTGRIVARLKEEAAKNRATPAPQATSPRRPSDGIGGQVLQGRPLSRRPAGQ
                     SENHRPPIR"
     gene            complement(34693..35775)
                     /locus_tag="WM704_000216"
     CDS             complement(34693..35775)
                     /locus_tag="WM704_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylate transferase"
                     /protein_id="HFA9339732.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
BASE COUNT         7961 a         9266 c        10396 g         8304 t
ORIGIN      
        1 attgccgttt tgtccaaaat gcgtataatc ctgtccatat ttctgctgcg ggctgattta
       61 ttttagacaa ggactaccat gcaattagat atagaccgtc tggtcgccta tttcggcggc
      121 gtgaacgcgc ttgccgaagc gttgaaacgg cacgatcctg aaaatgccgc gacgaccgcc
      181 gccatctaca aatggcgcac acgcggctcg ctgcctttgg cgcaactgca aaaattgacc
      241 gcgcttgccg aatcgcaagg aagaccgctg gatttgaatg cttttttaca aaaaaacgaa
      301 tctctggaga gaacagaaat gacacaggcc aaccgcgtta ttattttcga caccaccctg
      361 cgcgacggcg aacaatcgcc cggcgccgct atgaccaaag aggaaaaaat ccgcgtcgcc
      421 cgccagttgg aaaaattggg cgcggacatc atcgaagcgg gttttgccgc cgccagcccg
      481 ggcgatttcg aggcggtcaa tgcgattgcg aaaaccatta ccaaatcaac ggtctgttca
      541 ttgtcccgcg ccatcgagcg ggacatccgt caggcgggta aggccgttgc gcccgcgccg
      601 aaaaaacgca tccacacctt catcgccacc agccccatcc atatggagta caagctgaag
      661 atgaagccga agcaggtgat tgaggcggcg gtcaaagcag taaaaatcgc tcgtgaatac
      721 accgacgatg tggaattttc ctgcgaagac gcgttgcgtt cgcaaatcga tttccttgcc
      781 gaaatctgcg gcgcggtaat tgaagcgggc gcgaccacca tcaatattcc cgataccgtc
      841 ggctattcca tcccgtacaa aaccgaagaa tttttccgcg aactgattgc caaaacgccc
      901 aacggcggca aagtcgtctg gtcggcacac tgccacaacg atttgggctt ggcggttgcc
      961 aattcgcttg ccgcattaaa aggcggcgcg cgtcaggtgg aatgtaccgt taacggtttg
     1021 ggcgaacgcg cggggaatgc ttcggttgaa gaaatcgtga tggcgttgaa ggtgcgccac
     1081 gacttgttcg gcttggaaac cggcatcgat accacgcaaa tcgtgccttc gtccaaactg
     1141 gtgtccacca ttacgggcta tcccgtgcag cccaataaag ccattgtcgg cgcgaatgcc
     1201 ttttcgcacg aatcgggcat ccatcaggac ggggtgctga aacaccgcga aacttacgag
     1261 attatgtctg ccgaatcggt cggttggtcg gcaaaccgat tgagcttggg caaattgtcc
     1321 ggccgcaacg ccttcaaaac caagctggcg gatttgggca tcgagttgga aagcgaagag
     1381 gcactgaacg cggcatttgc acgcttcaaa gaactcgccg acaaaaaacg cgaaatcttc
     1441 gatgaagacc tgcacgcgct ggtgtcggac gaaatgggca atatgaatgc cgaaagctat
     1501 aaattcattt ctcaaaaaat cagcaccgaa accggcgaag agccgcgcgc cgacatcgtg
     1561 ttcggcatca aaggcgaaga aaaacgcgct tccgcaaccg gctccggccc tgttgatgca
     1621 attttcaaag cgattgagag cgtggcgcaa agcggcgcga ctttgcagat ttattccgtc
     1681 aacgccgtca cgcaaggtac ggaaagccaa ggcgaaacca gcgtccgttt ggcgcgcggc
     1741 aaccgcgtcg tcaacggtca gggcgcggat accgacattt tggcggcgac cgccaaagct
     1801 tacctttccg ctttgagcaa gctggaattt agtgccgcca aaccgaaggc gcagggcagc
     1861 ggtacgattt gagcgtgaaa acagacgatg ccgtctgaag cataaaaagg cttcagacgg
     1921 cattgcggcg atatagtgga ttaaatttaa accggtacgg cgttgcctcg ccttgccgta
     1981 ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt aaatttaatc cactataata
     2041 gggcgcaaaa cccatttgaa aaggaaaatg atggattccc gaaaatttac cgaagcatcc
     2101 aaacggcggt tgggcgaatt gttggatgcc aaaagcgaac aaggcaacac gatgcgttgc
     2161 gacgaggttc aaggttttat gacggcgctg ttgagcgggc cggacaaatt ggcaccgctc
     2221 gactggctgc ccgaagtgtt gggcgacgaa tcgcaattta ccgccgccga acgttccgaa
     2281 atcgaacggc tggttttggc aatggcgatg gaaacaaccg ccgcgatgtc ggataaaaag
     2341 ctgcccgatt tgtggctcta cgacgacggc gagggcggca gcgattttta cacttggtgc
     2401 aacgcctatc tttacggttt ggacattgtg ccgaccgatt ggtttgaagc cgccgatgat
     2461 gaagcgtttg aagagctgtt ttaccccgtg atggcattgg gcggcattta cgacgaagag
     2521 gaaaacggcg ctatccgcct gcaattcacc gaaggcgagc tggcggaact tgcgtccgaa
     2581 ctgccttacg cgctggcaga catctaccgc tactggcagg cggtcatcaa caaaccgcaa
     2641 accgtccgca gggaagggga aaaaaccggc aggaacgatc cctgtccgtg cggcagcggc
     2701 aggaaataca aggcgtgttg cggtaagaat tgaagcgttt gttttcatct gaaccaaacg
     2761 taaaaatgcc gtctgaaacc ggatttccat gtttcagacg gtatttttca caggcggtca
     2821 gtgctgtttt ttcatgccga accggacaaa gccgacgata cccaaaacaa tcatcgggac
     2881 gctcaaccat tgccccatcg acagccccaa ggtcagcagc ccgagatagt cgtcgggttg
     2941 gcgcgcaaat tcggcaataa agcggaacac gccgtagccg ccgagaaaaa gcgcggcagt
     3001 ctgcccggtc gggcgcggtt ttttggaaaa cagccaaacg acggcgaaca ggcagatgcc
     3061 ttcaagggca aactgataaa gctgcgaggg atgacgcggc agcataccgt attgttgcag
     3121 ccattctgcc caaagcggat tgtgcgcggc ggcttcggca tcttcgtaat gcgcttgcgg
     3181 gaagcccatt gcccaaaatg cgttaatgtc ggtaatgcgt ccccaaagtt cgccgttgat
     3241 aaagttgccg atacgtcccg aagcgagacc cagcggaacg agcggcgcga ccgtgtccat
     3301 cagtttgagg aagccgatgc cgtgcttgcg gctgaacaac catatggcaa taactacacc
     3361 caaaaagccg ccgtggaacg acattccgcc ttcccatacc ttgaaaatat caagcggatg
     3421 ggcgaggtag tcggagaatt tgtaaaacag gacatagccc aagcgtccgc ccaaaatcac
     3481 gcccaaaatg ccccatgtca ggaagtcgtc gagcgattct ttggtaaaaa cggacaagcc
     3541 ttgcgcgatg cgccttctgc cgagaaaggt aaaaagaata aatccgagga tgtagcttaa
     3601 ggcataccag cggacggcaa gcgggccgat actgatgagg acgggatcga attggtgatg
     3661 gataatcata acgggctttc gttttcaaat gccgtctgaa agacgcaaaa gggctttcag
     3721 acggcatttt cagtggggag tgtcagcgca aatcgccggc gacgttcaaa atggcggaaa
     3781 gcaccgcttc cccaagccgc aaagaacgct ggccgttcca gccgaagtcg tcgtcgggca
     3841 ggttggcgtt gtctttgaac ggcatttcca gcgtgtaggc aaggcagttg aaacggttgc
     3901 cgacccagtt ggtcgccaag gtcatattcg ctttgcccgg cgcgtctttt tcgtaaccgt
     3961 attcgtcttg gaaatcgggg ctggcgttca aaagggcggt tttgaactgc gcttccaacg
     4021 cggaaatgcg cggattgtag ttcggcacac cttccgttcc tgcaacaaag acaaagggca
     4081 gaccttcatc gccgtggatg tccaaaaaca aatccacgcc ggtttccagc atttttccgc
     4141 gcacgaagaa cacttcgggg cttttttcca aagtcgggtt ttcccattcg cggttgaggt
     4201 tcgcaccggc ggcgttggta cgaaggttgc ccagtgccga accgtcggga ttcatattgg
     4261 ggacgatgta gaacgtggcg cggtcgagca gggtgcgtgc tgtcgggtct tgcgaatcga
     4321 gcagcctgcc gagcagccct tcgacaaacc attccgccat ggtttctccc gggtgctggc
     4381 gggcggtaat ccagattttc atgtcgcttt cgacttggtt gcctatggtc agcagattga
     4441 tgtcgcggcc ttgcacggtg ctgcccaagt cgtcgatgcg gcacaggccg ctgccttgcg
     4501 cgtcgccgag gaggttgagg tgctgctctt cggaataggg ttcgaaatag gcgtaataca
     4561 cgctgttgga cagcggcgta tgattgacgg tcagtacgcc gttttcgtag gaagtcggta
     4621 cgcggaacca gttgcggcgg tcgtatgagg cacacgcctg atagccttcc cagcctttcg
     4681 ggtaggcggc ttctgccgcg ttttcaaaat gcatgatgca gttttgatag gccgcgcctt
     4741 gcagccggaa gtagaaccat tgtgcgaaat cggaggcgtt gtcggaacgc agggcgaggc
     4801 ggatgttgga agggtcggtc aggtctttga cgacgaccga gccggcatcg aagcgggtgc
     4861 tgattttaat catgggaaag tccttgctgt cgccggtttc tcgaaccgga taaaccgcga
     4921 ttttaccgcc cgtatcgcaa ggcttcaacc tgcccgaagg tgtgccaaac gccgtctgaa
     4981 gattgtttca gacggcgttt ggcgttaaca taagccgaaa ttgtcaacaa tatggagccg
     5041 ttatggagtt tgaaaacatt atttccgccg ccgacaaggc gcgtatcctt gccgaagcac
     5101 tgccttacat ccgccggttt tccggttcgg tcgccgtcat caagtatggc ggcaacgcga
     5161 tgaccgaacc tgccttgaaa gaagggtttg cccgcgatgt cgtgctgctg aagctggtcg
     5221 gcattcatcc cgtcatcgtt cacggcggcg ggccgcagat caatgcgatg cttgaaaaag
     5281 tcggcaaaaa gggcgaattt gtccaaggaa tgcgcgttac cgacaaagag acgatggata
     5341 ttgtcgaaat ggtattgggc gggcacgtca acaaggaaat cgtgtcgatg attaacacat
     5401 atggagggca cgcggtcggc gtgagcgggc gcgacgacca tttcattaag gcgaagaaac
     5461 ttttggtcga tacgcccgaa cagaatagcg tggacatcgg acaggtcggt acggtggaaa
     5521 gcatcgatac cggtttggtt aaagggctga tagaacgcgg ctgcattccc gtcgtcgccc
     5581 ccgtcggcgt aggtgaaaaa ggcgaagcgt tcaacatcaa cgccgatttg gtggcaggca
     5641 aattggcgga agaattgaac gccgaaaaac tcttgatgat gacgaatatc gccggtgtga
     5701 tggacaaaac gggcaatctg ctgaccaaac tcacgccgaa acggattgat gggctgattg
     5761 ccgacggcac gctgtatggc ggtatgctgc cgaaaatcgc ttctgcggtc gaagccgccg
     5821 ccaacggtgt gaaagccaca cacatcatcg acggcagggt gcccaacgcg cttttgctgg
     5881 aaatctttac cgatgccggt atcgggtcga tgattttagg cagaggggaa gatgcctgaa
     5941 gcaaagtcgg aaaatgccgg ctttggcgga aaacctgttt gtccggtttc tgtttttggg
     6001 gtttcgggca atttctaagt tgttattccc gagaaaacag aaaccacaac agaaacctaa
     6061 aatccgtcat tcccgccggg aatttggttt ttttgaaatc cggttgtttt ggataaattc
     6121 tccggctttg attttctgtt tttccgataa cgccataact ttgaaatttc gtcattcccg
     6181 cgcaggcggg aatccagacc tgtcggcacg gaaacttatc gggaaaaaaa ggtttcttta
     6241 gattttacgt cctagattcc cactttcgtg ggaatgacgg gatgtggatt tttgtgcgga
     6301 tttgaaccgg taaaggtggt gtgggattgg tggaatgacg aaatataagt ttccgtgcgg
     6361 acggatcaag attcccactt tcgtgggaat gacggtggaa agattgttgt ttttcccgat
     6421 gaattcctgt gttttttgtt tttccggatg aattcctgtg gctttgggtt tttgggattt
     6481 cagcctcaat gccgtctgaa cgccgaatcg ggcttcagac ggcattgcgt catttgaaat
     6541 tcaaaaccgg ccagcctttt tctttggctt ctttttccag ttcggcatcg gggttgacgg
     6601 cgacgggttc gctgacgagg cgcagcagcg gcaggtcgtt tttggagtcg ctgtaaaaat
     6661 aggttttgcc gtagctttcg agcgtttcgc cgcgttcggc gagccattgg ttcagacggg
     6721 tgattttgcc ttctttgagg ctgggcgtgc cgatgtaatt gccggtgtag cggccgtcgg
     6781 gaccggtttc gagttgtgtg ccgatcatgt tggcgatgcc gaaaaggcgg cagacggggg
     6841 tgatgatgaa ctcgttggtt gaggaaatca cgagggtttc gtcgcctgcc atttggtggc
     6901 tctgcaccag catacgctgc ataggcgaga tgtgggggat gatgtattcc gccataaatt
     6961 cgcggtgaaa ctccgccagc tcttctttgc tgtaacgggc gagcggggcg aggtggagtt
     7021 tgaggaatgc gtcgatgtcg aggcagccgt tttggtagtc gcggtagaat ttttcgtttt
     7081 gcgcttcggt ttcggcagcg tcaaccaacc cttttttgat gaggtattgc ggccaggcgt
     7141 ggtcggaatc ggtgttgatg agggtgttgt cgaggtcgaa gatggcgagg tttttcattg
     7201 ggtttcctgt tgtttcaaaa gctggcgaag cagcggcagg gtgatgcgtt tgcccatggt
     7261 tacggcgtag ttgtccagcg tgtcgagcat catcatcagg ctgtccatat cgcgccgcca
     7321 gtgtttgagc aggtattcga aaatttcgga atcgacggtt acttggcgtg ccgccgccat
     7381 attggcgagc gcgtcgattt tttcttggtc ggttagtggc ttgacttcgt aaacgaggca
     7441 gtatgccata cgcgtccgca aatcttcgcg gatgacgagc tgctggggcg tgtattccga
     7501 accgagcagc aaaaagcctt tgccgctgtt gcggaagcgg ttgaagatgg aaaaaagcag
     7561 ggcttgttct tcgttgccca gtttttcgac ttgatcgacg gcgaggtatt ctgcctcaaa
     7621 cgcggcatcg gtcagcggca tggaggcggc atcgatatag gcggcgtttt tgccggcttc
     7681 gagcgcctgt gcgacccacg cctgcaacag atggcttttg cccgcgcctt cttcgcccca
     7741 gacgtagata aacgggtcgt gtttatgctg taacacatag accagttcgg cattttccgt
     7801 gccgaggaat ttgtcgaaac tcggatagtc gtgtgcggca aagtcgaaaa taagctggtt
     7861 cacaaaggca taccggatgg gttggaatcg ggtttattgt acgttgtttt cgcgtgtctt
     7921 tccaatttga acgatgccgt ctgaaaacgg cttcagacgg catcgttcag ccgcaggcag
     7981 cgttgccgac atcgaggtgc atattgcgga acgcgttgag cgtacttcgg tgtccgatac
     8041 tgatgatgct gtcgggcagt ttttgcttta aggcgcggta gagctaggct tcggccggtt
     8101 cgtccaaggc ggcggcggct tcgtcgagca ggacgatttt gggcttggag agcagggcgc
     8161 ggacgaaggc gacgcgttgc agttcgccgg gggagagttt gtgctgccag ccgtctgttt
     8221 tatccaattt gtcaatcaga taacccaagc ggcaggtgtt catggcttcg gctaactcgg
     8281 gatgctgttt gtcgatatcg gggtaacaaa ccgcgtcgcg caggctgcct tgtgccgtgt
     8341 acgggcgttg cggcaggaag aggatgcctt gatgcggcgg acggctgact ttgccgctgc
     8401 tgccgaacgg ccaaagcccc gccagcgcgc gcagcagcga ggttttgcct aaaccgctcg
     8461 ggccgcgtat cagcagggaa tcgccgcttt tgagtttgat gttgatgccg tccaataaaa
     8521 cttcgccgtt gtggcggaac agggcgacgt tttccagtgc aatgccgtct gaaacttcgc
     8581 taatctcggg ttgctgcgcg ccgtgttgtt cttcggtact cagcaaaaag acgtacagac
     8641 gctccagtcg ggcgtggcag gcggtgaatt tgttgtagaa cattcggaag aaagacagcg
     8701 cgttttgcag tcgcgcgaag gcttggacgg tctgctggat gtcgccgatt ttgatttgtc
     8761 cggcaaacag gcgcggagct tgcaaaataa tcaggaagag tttgatgccg ttggtgaaca
     8821 tatcgttaaa gccgcttaag cagacgcttt gccgcgcgat gcgccaacgg ttgcggataa
     8881 tggctttaaa gcggtcggaa agccggccgt gttcgtgttg ttcgccgctg taaaacgcca
     8941 cgctttcggc gtggtcgcgt acgaggatga gggaataacg gtagtcgccg ttgagttttt
     9001 tgttttcata attgtaacga atcaaagggt tgcctatcca catggcgata aagatcgcca
     9061 aaatcatgaa aataaagaca aaccaaacaa tgccgtgcgg aatgtcgaag ccgaacacgg
     9121 tcaggatgcc tgccaagccc cacaaaacaa cggcaaattc cagagaagta acgaccgaat
     9181 tgaccatgcc gcgcacaaat tcgatggtcg aagcgatgaa ttcctgcgca tcctgttgga
     9241 tacgctggtc gatgttgtcc ggcgcgtggc ggcgcatttg caggcggtag tagtttttgt
     9301 cggcaagcca gcgcgaagtc aaaacttcat tgagccgttc cgaccattta atcgccaagc
     9361 cttaatcgag aaagtcgtta acgacgttgt taaacgcccg tatcagtacc acgccggcgt
     9421 tcatcgctgc aaacatccaa aatgccgaag cattcaaatc ctgcatcgaa tcgtaaagtc
     9481 ctttggacat gaaggtactc aacacattca accgcacttc ggttaacagc agcgtaacca
     9541 ttgccgtaat cagcagcaat actttgaccg tgcttttcgg tgtcaggcaa aggcgcagga
     9601 tataggcaaa ctcccgcccc aagcgcgtgt tgcgcgcgaa aaacagaatc accgccgagg
     9661 cggcggtaat catcaataag gtctgcaaca gccaagacgg cgtggaataa agctcggttt
     9721 gccggttttg catgggaaat ctcttacggt atcaatgccg tctgaaaaag acgggtacag
     9781 ttgatttttt gatgaagttt ggggaagttt tgccggtcag ggcatattgc ttgttatagt
     9841 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata gtacggcaag
     9901 gcgaggcaac gccgtactgg tttaaattta atccactata atttaccata caaccacgcc
     9961 ggaattaagt ttaaatttga ataaaaggtt cgggttctgc aaaatacaga acccgaacct
    10021 tgttcggata ttgaaaccgg tcgcctgatt ttgggcggtg cggcttgcaa gtatcaagat
    10081 tcgcatatgc cgtctgaagc tcggagaggt tcagacggca tatgcttatt tgggctgctc
    10141 ttcgacgaat ctcggacctt tcaagatacc gttgcgggaa tagggcgaca gtaggttgta
    10201 tgcggcggtt ttggaaacct gataaccgcg gtcggtcagg ctgttggcaa tctgattgac
    10261 cactgcgccg accaaagccc ccaacaggcc gctgttgctg ttgttgctgc cttcgcggat
    10321 gctggccgaa cccgaccaca actctttccc gttgcgggaa tcgaccagcc gtgctttggc
    10381 ggatacggtc gtcacgctgt ctaaaatttg atatgaagtg ccgtattcgg taaccgtaat
    10441 gtacaaaacc gcatcattgc cgaaaatttg atgcagtttt tccggccgga cggcgtgaat
    10501 atcggcggca ttggtcaagc cgttttcttt gaaggtttcc tccacgactg cggcggggaa
    10561 gacgtaatag ccggcttcgg aaatcggcgc ggcggtcgaa gccagcatcc cccaagtgcc
    10621 gttgacatca ggcgactcgt tcagcggcgg aaccaccaaa attgaagccg gtttgctttc
    10681 tttgaatgac gtgtagtcga ggtcgggagc ttttcgaact tggcaggcag acagagccaa
    10741 cacggcggca agccctaaaa tcaaaggttt catcgcttgc ctcctttacc ggttttcatc
    10801 aggaagtcca taaatacgcc cgattcggga aacagccttt tctcttcttc aaattggcgg
    10861 aacgcgccct ctttgtctcc cgaacgggaa agcagcagtc ccaaatgggc gtgcgcaccc
    10921 ggggcggcat tcattttttt tgttggcggc ttccgcaaag tatttttcca tcttttcggt
    10981 ctgcttgccc aacgaagtgt cgtcgttttt caaaccttca tagacggtat cgggataacc
    11041 gccgtaataa tacaggtttt tatgcccgcc gccgcaggcg gtcagagcca agaccgccgc
    11101 acacagcgac aaacggccca aggttttcgg attcatcatt tctccttaac ggttggattg
    11161 ccatgcgccg ttgtcgacag cctgaaccaa gttgtcgacg gcttcgcgga ttgccaagtc
    11221 taaaactttg ccgttcaaag tcgcatcgta gccggaagtg ccgccgaaac cgatgatttc
    11281 gcggttggaa agtgcgtatt cgcccgcgcc ctgtgtggaa tagacgattt cggaagtatt
    11341 gacgttgacg atattcagag ccacttttgc ataggcgatt tgcgatttgc cgcgacccaa
    11401 aatgccgaag agctgatgat cgccgacatc tctgcgtccg aattcggtta catcgccggt
    11461 aacgatataa tctgcgcctt tcaggttctg cgctttgccg gaaatgccgg attcctgttt
    11521 caatgcgcta aggttggtgc ggttcagtac gttgaagcgg ttggtttgtt gcaggtgtgt
    11581 taccaggatg gtttttgcct ggctgcccag acggtcttca ctgtcggaga aaatgccttt
    11641 ttggaagctg gagcggttgt cgaatgttcc gacggaaatc ggggtgcgaa caccgtgata
    11701 ttgcgtattg caggaggcga cttttgcaac ctcgaggctg cgtgaggact cggtcgcaca
    11761 gccggtcagt gaaacggcag cggcggcaaa agcgatggcg gcggaaacgg ttttcataaa
    11821 atttacccta aggtccagtt agggaaataa cgggttatca ttattgtcct tatgtaaatt
    11881 taagtcaagg tgtttgtctg tgcgggacgg atgcgcgcgg aaggtacgga tatttttcaa
    11941 aacggaagcg cggcgcgggc gggatgcctg ccttttccgg atttagaggt aaacgatttc
    12001 gccgctccgc cctttgcttt cggcacttgc ccaccagaca aatgcgggca gtacgtcccc
    12061 gtagcttttg cgttcgcttc cggcttcgcc cggatgggat ttcatgcgtt gcggggaatt
    12121 gatggggccg gggacgagga cgttggcgcg caggttgccg aagcgttccc attcgtcggc
    12181 ggcgactttg cacaggtagt tcaacgcggc tttggacgcg ccgaagctgc cccagtaggc
    12241 tttgggtgtt tcgccgtggc tttcgccgac gaagatgacg gacgcgtcgg gcgactgctt
    12301 cagcagcggg aacagggcgc gggtcagccc catcggggcg acggtgttga tgcggtattg
    12361 gttgacccat tcggcgacgg tttggaaatc cagcggcgag agggcgtaaa aatagccggc
    12421 gcagtggacg atgccgtcca gtttgccttg cgtggcttcg gcaatggtgg cggcaaaccg
    12481 ttcgaattct ttttcttccg cactcatcag gtcgaaacgg atggcgaagg gttcggggtg
    12541 tccggcttcg acaatcgcgt cataggcttt ttccagtttt ttctgatggc gtgccaccag
    12601 aattacggtc gccccttcgg cggcgtaggc tttggcgacc tgttcgccca agccttgcga
    12661 tgcgccggta actaagatgg ttttgtcggt cagtgtcggc atggttgtcc tttgggtttg
    12721 acggttaatg tattttagcg ttttgccgca ccttgtaaag cgtcccgatg ccggccggcg
    12781 ttttcagacg gcatcgttca ggctttgcag gcaggcttcc gccgacgcga aacctgattg
    12841 tacggcggct tcgagcgtgg cggggtagtg cgggtggagg tagtcgccgg cggggaagat
    12901 acggtgccgg tgcagccacg acaaatccgg cggcggggca tcggttgcgg ttgtggcgcg
    12961 tttttcggtg atgacgcgca cggcttcggg ttcgcccaaa tgcggaagga tgcgtctgag
    13021 gtcggcgtgg gctttgtccg cccacgcccg gtttgcaaac gcgccgacgc ggtcggaaac
    13081 gctaatgacg gcggagactt cgttttcggg cagtccgagc ctgccccggc aaagcagcca
    13141 ttgtgccgtg ccgtcggcaa tgccggtcag cggggcgggc aggcggacgg gttcggcgta
    13201 gcgcagatag acggtggtaa tggcgtggta gcgaaggttt tgatatgccg tctgaacgtg
    13261 ttcgggcgtg ccttcgggca ggagcgcggc ggcgtggtag ggcgcggtgg caagtatggc
    13321 ggcatcgaag gcttcgccgt tgacgaggac ttttccgtcc gggagggtgt tcagacggca
    13381 tacgcgcgtt tcgaggcgga tgtccgcgcc gagccgttga agctccgcca aggcgggttc
    13441 ggcgacgatt gcgcccaaat cctgtttggg taggagatag tcgctgccgg attttttcgt
    13501 cagcacgccg tcggacaaaa cgttgcacaa cacgcgcagg cttgcggttt ccaaaggcgt
    13561 gttgagcgcg ccccagacca agggctgcca aaactgcatt acggcggcac gcggcacgtt
    13621 ccgctgtttc agccattgtg caactgtcgt gtcgggctgt ccgaggcgtg cggacttctg
    13681 caaatcggac atatcggcaa gcagtttggc tttgaatgcg gacggtacgc gccgggcaag
    13741 cagcacgccg cccaaaatat gcagcggcgc gggcaggggg agggcgcgga actgcaaacc
    13801 gccgtgcata tgccagtgca gcggtacgcg caaaaaggcg gcacgggggt ctgaaccgat
    13861 ggttttcatc aggcgcaaca cgccccggta tgcgccgagc aaaatgtgct gcccgttgtc
    13921 caaaaaaccg aaaccgtcgg tatttccggc cagtgtgcgc gcccttccgc ccgcctgccg
    13981 gccggcttca aacagggtaa cgtcggcgtg ccgcgccaag gtgacggcgg cggacaagcc
    14041 ggcccagcct gcgccgatga cggcgatttt cgggcgcgga tgcggcgtgt tcatcattta
    14101 ttcctccaat ggttgaaacc ccgtttttcg gggcggcggg ggttgggttt tcctgaaggg
    14161 cagtatgcgc cttatgcccg ttccggcgtg ccggggcgcg gtttgaatcc gaataaccag
    14221 gttttcaggg caatgcgttt tttgcgcggc gaagggaggg cgattttgta tttgaggacg
    14281 ttttgcgcgc cgtctcggtc gatttcgttc aacagcgcgt aataaaccgc cgccataacc
    14341 agtccgactt tttgggcttt tttatcggca tcaggcagca gcgatacggc ttcgcggtag
    14401 gtttcacggg cgcgtttgat ttggaacgcc atcaattcgg caaaattgcc cgtcgggctg
    14461 cattgcaaaa tcacgcttgc gggtacgtca aaccgctgca tttcctccat cggcaggtaa
    14521 atccgtcccc tgcgcgcatc ttcgccgaca tcgcggatga tgttggtcag ttgcagcgca
    14581 agacccattt tatccgcgta ttccagcgtt ttgccgtctg aaaaccccaa aatccgcgca
    14641 atcaggcagc cgaccacgcc cgcgacgcgg cggcaataca gtttcaattc ctcaaaactg
    14701 ccgtaacgcg cctgaaccaa atccatctgc atcccgtcga ttaaggcttc cagttcatat
    14761 ttcggcagct tgaaggtttc cttaacctgc cgcaaggcct gattgacggg gtgttccggc
    14821 atcgcgccgc cgaacgcatt gtccaaatcg ccgcgccacc agttcaatgt tgcctgtgca
    14881 acatcggggt tggaacattc gtcaaccaca tcgtccaatt cgcggcaaaa gacatacaga
    14941 accgttaccg catcctgttt ttcctgagtc aggaaacgga agcccgacaa aaaactggag
    15001 cggctttcct ctgctttttg gcggcaatag tcgagtcctt tcacgattta tattcctaat
    15061 gatgggcggg aaaggcggat tttatcggta tttggcggta gagggcaatt tcggcggcac
    15121 ggcccaatcc ttagcggttt gctcaactat cggcgcaaat tctgttaaaa tgccgccttc
    15181 cttcctttac acaccgcacc gacaggcaaa atttatggct cttttgcaga tttcagaacc
    15241 gggtatgtcc gccgccccgc accggcaccg tttggcggca ggcatcgatt tgggtacgac
    15301 caacagcttg gtcgccaccg tccgcagcgg cagtgccgcc tgcctgcccg atgccgacgg
    15361 gcgcgttacc ctgccttccg tcgtccgcta tctggaaaac ggcggcattg aagtcggcaa
    15421 aaccgccctg tccgcccaaa aaaccgaccc gctgaacacc gtcagctccg ccaaacgcct
    15481 tatcgggcgg actcttgccg atctgcatca aaatacgcat tacctgcctt accgtttcgg
    15541 cgacaatcaa cgctttatcg aactgcatac gcggcagggg gtgaaaacgc ctgtcgaagt
    15601 gtcggcggaa atcctcaaaa ccctcaaatt gcgtgccgaa gaaaccttgg gcggcgattt
    15661 ggtcggcgtg gtgattaccg tccccgccta tttcgacgac gcgcaacgcc aggccaccaa
    15721 agatgccgcg cgtctggccg gtttgaacgt attgcgcctg ctcaacgaac ctaccgccgc
    15781 cgcaatcgcc tacgggctgg acaacgcctc ggaaggcacg tttgtcgtgt acgatttggg
    15841 cggcggcaca ttcgacgtat ccgtgttgca actgaccaaa ggactgtttg aagtcaaagc
    15901 caccggcggc aacagcgcgt tgggtggcga tgacttcgac catcgtttgt tctgctacct
    15961 gctcgaacaa aacagactct cccaactcaa cgaacaagac agccaactcc tgctctcgct
    16021 cgtccgcgcc gccaaagaac aattgaccac gcaaaccgaa gcgcgcattc aggcgacgtt
    16081 ttcagacggt atggcaatcg acacaagcat cagtcgcgcc gagttccaca acctgacgca
    16141 gcatttggtg atgaaaacgt tcgagccggt caaacaggcg ttgaaagatg ccggtgtcgg
    16201 taaaaacgaa gtcaaaggcg tggttatggt cggcggttcg acccgtatgc cgcacgtcca
    16261 acaggcagtc gccacctttt tcggacaaac cccgctgaac aacctcaatc ccgacgaagt
    16321 cgtcgcgctc ggcgcagcca tacaggcaaa cgtcctcgca ggcaacaaag ccgacggcga
    16381 atggctgctg ttggacgtta cccccttgtc gctcggtttg gaaacctacg gcggcttggc
    16441 ggaaaaaatc atcccgcgca attccaccat ccccaccgcg cgcgcgcagg actttaccac
    16501 cttcaaagac ggtcagaccg cgatgacgat acacgtcgtg caaggcgaac gcgagctcgt
    16561 ttccgactgc cgcagccttg ccaaatttac cctgcgcggc attccgccta tggcggcggg
    16621 tgcggcgcgt atccgcgtta ccttccaaat cgatgccgac ggtttgctgt ccgtttccgc
    16681 ccaagaacaa agcaccggcg tacaggcgca aatcgaagtc aaaccctcct acggtttgga
    16741 cgatgacacc atcacccaaa tgctcaaaga cagcatgggc aacgccgccg aagatatggc
    16801 ggcacgcgcc cgcgccgaag ccgtggtcga agccgaaagc ctgaccgatg ccgtcaacgc
    16861 cgccctcgag ttggacagcg atttgctgga tgccgaagag tttgcacaaa tccagcgaga
    16921 catcgccgat ttgcaaggcc gtctgaaaga cggtaaagct gaagacatcc gtgcagctgt
    16981 cgccaaactc agccgcagca ccgacaattt cgccgccaag cgcatgaacc gcaacatcca
    17041 acgtgcgctg acaggtcaga gtgtcgataa tatttgatac ttaaacggtt tcagacggca
    17101 tagatataac ctgatgccgt ctgaaggatc gaaaacactt gaaaaacatc gatatggaaa
    17161 agtcaggcat tgtctattcg atgaaaaccg tcatcaaggg cgtgtatagt gaattaaatt
    17221 taaaccggta cggcgttggc tcgccttgcc gtactatttg tactgtctgc ggctcgccgc
    17281 cttgtcctga tttttgttaa ttcactatat aaaatcggca tttcggacgt aagcaatttt
    17341 gaaggcagaa tgcgccattt ggaaaacaac ggctatgcga acgttgccgg gttggaacgc
    17401 ctcctcgccg tcaaaaccga caattacaaa gaaaaaaaac ctgctccatg aaattttcag
    17461 caaaagcagg ataggcgata ccgaattgtt cgccgtggac gaaaaccttg tgaaacgttt
    17521 gtttttatcg cttcgcggcg aaatcgtgtt cccgaaaaac gaaacggcgg aatcggaatt
    17581 tgaaaaaagc gtccacgaac gccggcagga agggaatgcc gggtccggcc gcaaacaact
    17641 gcttgatttg gtacggcgcg gacaccggga atacccttac gcgctgcccc ggcttttggc
    17701 ggacgcggca tcctacaagc cgaaaaaatc gaaaatccgc ctttttaaag aagcatattt
    17761 cggcaaaagc ggcacgaggc tgaccgacga aattgcagac ggcatccata tttacacctg
    17821 tttttcgcgg gcggatttgg aaaaagccta ttccgaatat ttggaacttt tcaaatccga
    17881 atcggatgcc ggaggtagaa aaccgcggta aggtgcaaac agataccgta cacgttgagg
    17941 agcagtatga tgggcgattc cgtcatttat tatgtagaac aggcagacga accggtaaac
    18001 cgtgccggcg aacgcgcccg taaaacattc aaatactttt ggcgcgagct tttttgggaa
    18061 cgccgccgca ttatttccgc cttggatttt gccatggtca aagtcccttt tttccaagac
    18121 ggcgaagacg gcgaaatttg cgaacatatg tggatcgacg atatttattt tgacggtctt
    18181 tatatttacg gtgtgctgaa caatgaaccc ggcggactga ccaatgtcga acaaggcgaa
    18241 agcgtttgcg ttccggttgg cgacatcagc gactggatgt tcgtgtgcaa cggcatcccc
    18301 tacggcggct ttaccgtaca ggcaatgcgc ggacagatga cggaagagga gcgtaccgaa
    18361 cacgataccg cctggggaat cgatttcggc gatcccgggc aggtattgcc ggtgtatgaa
    18421 gaaaaagaac atcccgaaaa tttggaagag catccgatgt gccggaactg tattgacgat
    18481 tttcggcaac agttgtccca aaatccggat tttctgcatg agcaggacga agacggctat
    18541 acgccgcttc atcatgaagc catggcagga aatgcactta tggttcaagc catgcttgaa
    18601 tacggcgcaa atcctgcctc aaagacatcg gaaggctata ccgccctcga ttttgcccgc
    18661 ctgacgggct ggcaaaatgt tgccgacctg ctcgaaccgc gacattaggc agacagtttt
    18721 cctaaaaacg gacacaaata ctttttacag aaagacaata aaaatgccca aaatcaccgt
    18781 acttccacac gcaacattat gccccgaggg tgcagtcatc gataacgcgc ccgaaggcaa
    18841 aaccgtcctc gacgtgctgc tcgaccacga catcgaagtc gatcacgcct gcgaaaaatc
    18901 ctgcgcctgc accacctgcc acgtgattat ccgcaaaggt ttcgacagtt tggaagagcc
    18961 ggccgaattg gaagaagacc tgctcgatca ggcttggggt ttggaagccg attcgcgcct
    19021 gagttgtcag gcggttgttg ccggcgagga tttgattgtg gaaatcccca aatacaccat
    19081 caaccacgcg cgcgaagaac actgaaaaca ggccgtctga agccggcacg cttcagacgg
    19141 cattgttgcg cggataaggc gcaattaccg aaaaacagac gtttgtgaag acggaacttt
    19201 cgattctata ttgattttta tcggtttttt gacggaacga cccgatacgg catctgaggc
    19261 ggcttgcgtt tcttcctgcc cgcctgccgg atttgcctgt ccttgcgcga aaccgaaaga
    19321 gacggcggcg cggcggacaa ggtcgagata gcgtccttca agctcggggc aggcggcgga
    19381 cacgctttct gccgcaaccg tgaagccgcc cgaccgactc ggaaaggcgt tccgcaatct
    19441 gcatgcagat gcgccaacgc cgggcgcgca acattgccgc atcgcaaccg acagccgctt
    19501 tatccaaagc aatcagaccg cgcctgctcc ggtagttaag gcaaaccgtt actctgtaga
    19561 gggtatgttg cccgtccaat atttgataca gtttgcgtat cagcagaatc aggcggtcaa
    19621 actcctccat ctccgacagg gagtcgggat ggtcggaagc ggtataaacc agttcggaca
    19681 attttgcggc aaacatattg ttcatcaatc ttccttatcg gttgaaaccc cgctcttcag
    19741 ggcggtagaa tcagatttgt ttgggagggg taactcctcc cggatcagga cgacacataa
    19801 ggtggtgctt gatgtgccgt ccggcgagtt gaaacattca gccatcctca aggggcggca
    19861 gttttgccga aacatattct acacggcttc aatgccggac gataaaagga aatttatatg
    19921 aaatggaccg atacccggcg catcgccgaa gaactctacg acctgcacgg cgaagccatc
    19981 gatcccaaaa ccgtgcgctt tacccaactg cgcgacctga ttatggcatt gcccgaattt
    20041 gacgacgatc ccgcccgttg cggcgaacgc atccttgaag ccgtgcagca ggcatggata
    20101 gacgaggcgg aataagtttc aggaatgccg tctgaaatgc ggcggtacgc ggttcgtgct
    20161 tctgtttgca gcgggaatgg ttttaccagt ctcctttttt cagcctgtcc agttggcggc
    20221 ggtcgcgctt ggtcggtctg ccgtcgggat gggcggaagt gatgcggctg aattggtcga
    20281 gctgtttgca ctcttccctc aatgctgccg ttttcgcgtc ttcttcatac agaagccgcg
    20341 cctcgggtgc cgggcggcgt tggtggttca aacctttaac cttgatttta tggggaaggg
    20401 aattgagcgt caggtcgata atatcgccga tgtctatggt tttactgttt ttgactttcg
    20461 agccgtttac ttgaacccta cccagttcga tatgcttttg cgcaagggaa cgggtcttga
    20521 aaaaacgtgc cgcccaaagc catttgtcca gccgcatggc ggaagaatcg tgcttgtctt
    20581 tcatacgatt ttgtttgaaa taattgaatt tgtttcgagt ttagcataag atacatcgcc
    20641 ttataactag tatatatgca ctaatccgct gttttccatg ctgtccgaac acaaaaagag
    20701 ggtgtggaaa agccgttttg gacaataaat taactgcgga atatgcacaa atagcgtatg
    20761 atagcggcag aatctgttga tgagagcttc attttatgaa acctgttttt ttggattttg
    20821 aacaacccat agccgaactg accaacaaaa tcgatgagct gcgtttcgtc caagacgagt
    20881 ctgccgtcga tatttcggac gaaatacacc gtttgcagaa aaaaagcaac gatttgacca
    20941 aatcgatttt cagcaaactc acacccgctc aaatttcaca ggtttcccgg catccgcagc
    21001 gtccctatac tttggattac attgacgcgc tgtttaccga ttttgaagag ctgcacggcg
    21061 accgccactt tgccgacgat catgcgattg tcggcggatt ggcgcgtttc aacggacaaa
    21121 gcgttgtggt cgtcgggcat cagaaagggc gcgacaccaa agaaaaaatc cgccgcaact
    21181 tcggtatgcc ccgtcctgaa ggctaccgca aagccctgcg cctgatgaaa acggcggaaa
    21241 aattcggctt gcccgtcatg acctttatcg atacgccggg cgcgtatccc ggcatcggcg
    21301 cggaagaacg cgggcagtcg gaagccatcg gcaaaaacct gtacgaactg acccgactgc
    21361 gcgttcccgt tttgtgtacc gtcatcggcg aaggcggttc gggcggtgcg ttggcggtcg
    21421 ccgtaggcga ttacgtcaat atgctgcaat attcgaccta ttccgttatt tcccccgaag
    21481 gctgtgcttc gattttgtgg aaaaccgccg aaaaggcggc tgatgcggca caggctttgg
    21541 gtattactgc cgaccgcctg caaaagctgg acttggtcga taccgtcatt aaagagcctt
    21601 tgggtggtgc gcatagggat ttcgggcaaa ccatgaaaaa cgtaaaagcc gttttggaaa
    21661 aacaactgca cgaagcgcaa agcatcccgc ttgccgattt gctttcgcgc cgtttcgacc
    21721 gcattatggc ttacggcaaa ttttcggaac aataattcgg gtagaacaag cagcaagcag
    21781 tttgtctgaa actgcttgct ttttctttat cgggatgaag ccgtgctgac tttagatgcg
    21841 tttgagcaat gcttgaagga ttgttttcct caaggtctga atggaaaaaa aacagcagtg
    21901 gcattaagcg gcggcttgga ttccgtcgtt ttgctgcatc tgctggtttg tgccgggaag
    21961 cgggcaggat ttgtgccgga ggcattgcac atccatcacg gcttgagtcc ccgagccgac
    22021 gattgggcgg atttctgccg gaattattgc gatatgctag gggtggggct ggaaacggtt
    22081 aaggtctgcg tggaaaaaaa cggtttgggc atcgaggcgg cggcaaggca aaagcgttat
    22141 gccgagtttg ccgaaaaagg ctttgacgtt ttggcgttgg cgcaccacag ggacgatcaa
    22201 atcgaaacct ttatgctggc agtcgcgcgc ggcggaggtt tgcgcgcttt ggcggctatg
    22261 cccgccgtcc gccctttggg ggaaaacggc attatctggc ggcctttgct gcctttttcg
    22321 cgccaagaca tatgggatta tgcccgaaaa cacggtttgc cgaatatcga ggatgaaagc
    22381 aataccgata cggcttattt gcgaaaccgc ttccggcacc gtattttgcc cgaactttcg
    22441 gcgcagattc cccatttcgg gcggcatgtg ctgaacaatg tccgcgcttt gcaggaagat
    22501 ttggctttgt tggaagaggt cgtcgttcaa gactgccgtt gggtttgcgg ggccggttat
    22561 ttcgatacgg cgcggtggct gacgttttcc ccgcgccgga aaacccatat tttgcggaat
    22621 tttctaaagg aaaacggcat ccccgtgccg aatcagaacg cacttgccga cattgcccgc
    22681 gttttgacgg aggcaaaaac cggacgttgg aacttgcaag gctttgaatt gcatcattat
    22741 gcaggcaggc tgtttgtgtt tgcgtcggaa cagttggcaa aaccggcttt tctgaaagat
    22801 gggacgataa gcggaaatct caaaaaaata ctgacagaac atagatttgt gttgaagcgg
    22861 catccgttcg gacttcctga agccatgttg gaacaggacg ggattttgag gacggtagcg
    22921 gcatcggata cgttggctgt cggcggcatc cataaaaatg tgaaaaaaat ccttcagggg
    22981 aaacgggttc tgcctttcct gcgcccaatt tggccgctcg ttgccgacag cggaaaccgt
    23041 ccattggcgt tggcaaactg ttgtgcggat tttcaaatct cggtttcaga cggcattttg
    23101 cccgtacatc ctgactttcc cattttattt tgataatatc gcaaacagat ttcggcggcg
    23161 ttcagtcggg tattgtccgg ttgcatattt ctaaaaggct tgtgaagtga aacacatcag
    23221 ttcgaccaat aatgaacata tcagacacct gcaccgcctg ttgtcgcaag gaaagttcag
    23281 acggcagtat gcccaaaccg ttttggaggg cgtgcacctg cttcaggttt tcctgcaatc
    23341 cggcagaaag ccggtcgggg tatatattcc cgaagctaaa atgccgtctg aagaagtcct
    23401 taaattgaca gcggttttgc cggaagacgg gattttttcc gtttcagacg gcatattgaa
    23461 aaaaatcagc agcttgagtt gtgcagacga tatacttaca ctgattgata tcccattggg
    23521 cggaaccttg ccggacaaag gcgactgtgc ggttttggac ggcgtgcaag atccgggcaa
    23581 tgtcggcacc gtcttacgga gtgcggcggc ggcaggggta ggtacggtcg ttttgggcag
    23641 gggttgtgcg gacgcgtggt cgcccaaggt attacgcgcg ggtatgggcg cgcatttctt
    23701 gttggacatt tatttgcagg cggatttgga aatatggttg gcgcactatg aagaccgtgt
    23761 gtttgccacc gcgttgcgcg aggaaaagca ggcggttttg tacggcgaag atttgtgcga
    23821 accgacagct tgggtgttcg gcaacgaagg tgcgggtgtc ggtaaagcag ttttagacag
    23881 ggcggacaag tgtgtcagga taccgatgca cgatgcaacc gagtctttaa atgtcgcgat
    23941 ggcggcgaca atctgcctgt ttgaacagat gcgccaacgg gcggcgtatt gaggaagaga
    24001 aatgccgtct gaaaaaatct attacggcgt attgattttc ttatgtatcg cttctatgct
    24061 gctgtcaccg tttttttatg cgggtgcttt gaagcccaag aaggcggcat tgcggaagga
    24121 cgggcagtgg aaactcatct tatcgtccaa tgccgtggcg gcggcggttt tgttttggat
    24181 atggtggaaa tggttttgac agatattgct caaaatcgtg ctaatggaat ccgaacaaat
    24241 aaagaatttg gtaaaaaatt tgttaaatca acggattaaa gttttgtgga aaacaaaaca
    24301 gctctaagca aatagggcgt ttgtcggtaa atacggaaga gttgcggcat tatcgggcat
    24361 ctttaacaag tagtgtcggc ttggcaggca atcggttttt atgggcagct tgcaaaatcg
    24421 cggatataaa attgcgaatc ggttaaagtg tggggacgct atgaaaaatt gcgaattttt
    24481 ttatgatccg acaagggcaa tctatgatag cggtgcagat tacttaacta gggaaaaata
    24541 tagattagtc gtaatcgcaa atagcgcttg ggggctattg ctgaatttac cttgttatta
    24601 tgacgaggtt ttggaaaagc ggaaaatacc gttcggcaaa caggaaattg atgacgatat
    24661 ggacaaagtg tccgccctta agcggaagtt taaagacatt tctgaaatca aagtagggga
    24721 tggttgggaa tacccgttca attatgagca aggaatgaaa gaattagatg aagttctatt
    24781 gaaatacatt cctttttttg aagagaaaca ataaaggagt ttaatatgcg cgtatctaaa
    24841 atgattggaa gtatattgct tgttgcagcg gttcagaccg tattttcggc aaatgtttac
    24901 gagtgccgcc ataatggtaa aaccagttac agccaaactc cgggaaaaca ttgtaccaac
    24961 gcgggtttgg ggcgggatcg ggtgtacagt tcggtcaggc ctgccgtgaa agacagggcg
    25021 gaagacgcag gagttggcga ttattcggac acggtgaggg acgaagccgt ccaaaatccg
    25081 aaagggaatg cacagaaaga cggttcggat gccggcatca gaccgcattg attgaaaccg
    25141 aatcaaccat tacggtgttg aacaatagaa tttgaacgat tagggaactc tgatgaaaca
    25201 catccacatt atcggtatcg gcggcacgtt tatgggcggg attgccgcca ttgccaaaga
    25261 agccgggttc aaagtcagcg gttgcgacgc gaagatgtat ccgccgatga gcacccagct
    25321 cgaagccttg ggcataggcg tacacgaggg cttcgatgcc gcgcagttgg aggaatttca
    25381 agccgatatt tacgtcatcg gcaatgtcgc caggcgcggg atggatgtgg tcgaggcgat
    25441 tttgaaccgt gggctgcctt atatttccgg cccgcaatgg ctggctgaaa acgtgctgca
    25501 ccatcattgg gtactcggcg tggcagggac gcacggcaaa acgaccaccg cgtccatgct
    25561 cgcctgggtc ttggaatatg ccggactcgc gccgggcttc ctcatcggcg gtgtaccgga
    25621 aaatttcggc gtttccgccc gcctaccgca aacgccgcgt caagacccga acagcaaatc
    25681 gccgtttttc gtcatcgaag ccgacgaata cgacaccgcc tttttcgaca aacgctccaa
    25741 attcgtgcat tatcgcccgc gtaccgccgt gttgaacaat ctggaattcg accacgccga
    25801 catcttcgcc gacttgggcg cgatacagac ccagttccac cacctcgtgc gcaccgtacc
    25861 atccgaaggc ctcatcgtct gcaacggaca gcagcaaagc ctgcaagata ctttggacaa
    25921 aggctgctgg acgccggtgg aaaaattcgg caccggacac ggctggcaga ttggtgaagt
    25981 caatgccgac ggctcgttcg acgtattgct tgacggcaaa aaagccggac acgtcgcatg
    26041 ggatttgatg ggcggacaca accgcatgaa cgcgctcgcc gtcatcgctg ccgcacgcca
    26101 tgccggagtc gatgttcaga cggcctgcga agccttgggt gcgtttaaaa acgtcaaacg
    26161 ccgcatggaa atcaaaggca cggcaaacgg catcaccgtt tacgacgatt tcgcccacca
    26221 cccgaccgcc atcgaaacca cgattcaagg tttgcgccaa cgtgtcggcg gcgcgcgcat
    26281 cctcgccgtc ctcgagccgc gttccaacac catgaaactc ggcacgatga agtccgccct
    26341 gcccgcaagc ctcaaagaag ccgaccaagt gttctgctac gccggcggcg cggactggga
    26401 cgttgccgaa gccctcgcgc ctttgggctg caggctgcgc gtcggtaaag atttcgatac
    26461 cttcgttgcc gaaattgtga aaaacgcccg aaccggcgac catattttgg tgatgagcaa
    26521 cggcggtttc ggcggaatac acaccaaact gctggacgct ttgagatagc ccgggcaatg
    26581 ccgtctgaaa gccctttaga cggcattgcc cggctgcgcg gcacaaaggc ggaaaaaccg
    26641 tttgccccgt attttcaaac gcgttacact tgccgccgct gttttcagcc atttgattac
    26701 ccgcaaccgc cgtcattgcg ccggcggttt gcctgtcagc gtcattgcgc cgctgtaaat
    26761 acgaaagaac acattatgac cgtatccccc gtcgccttgc gccgtaagac cgagtgcaag
    26821 cctcatccca ccgcgcgcta ttggaaaaaa tgcgatgtcg aggcactttt cggactgccg
    26881 tttttagaac ttgtctatca ggcggcagaa gtccaccgcc aaaatttcaa cccgcgcgaa
    26941 atccagcttt ccacgctgtt gtccatcaaa accggcggtt gtcccgaaga ttgcgcctac
    27001 tgtccgcaat cagcgcacca caacaccaat ttgggcaaag agcagatgat ggatgtggat
    27061 gaaatcgtcg aaaaagccaa aatcgccaaa tcgcgcggcg caagccggtt ttgtatgggc
    27121 gcggcatggc gcggccccaa acccaaagac gtggagacgg tttccgcaat catcaaagcc
    27181 gtcaagggct taggtatgga aacctgcggc acgttcggta tgctcgaaga aggtatggcg
    27241 gaagacttga aagaggcggg cttggattat tacaaccaca acctcgacac cgaccccgac
    27301 cgctacaacg acatcatcca cacacgccgg cacgaagacc gtatggatac cttgggcaaa
    27361 gtccgcaacg ccggtttgaa agtctgctgc ggcggcatcg tcgggatgaa cgaaacccgc
    27421 gccgaacgcg ccgggctgat tgccagcctc gccaacctcg acccgcagcc cgaaagcgtg
    27481 ccgattaacc ggttggtcaa agtggaaggc acgccgcttg ccgatgccga agatttggat
    27541 tggaccgaat ttgtccgtac ggtttccgta gcgcggatta cgatgccgca aagttatgtc
    27601 cgcctctcgg cagggcgcag caatatgcct gaagcgatgc aggcgatgtg cttcatggcg
    27661 ggtgcgaact cgatttttta cggcgacaag ctgttgacca caggcaatcc tgatgaggac
    27721 ggcgatagaa tcctgatgga aaagcttaac ctgtatccct tgcagtttga gccggaaggc
    27781 gaggtcgccg aagtggaaaa agcctccggg attaaagcgg attattgacg attgaaaaat
    27841 gccgtctgaa acttgaatgc cgtctgaaac ctgaaaaaag gctttcagac ggcatttgtt
    27901 cggacggaat ttccaatatc tttttaccgg cgcgtgatgt cgccgccggg cgagacatcc
    27961 agtccgttcc cgttggggaa gtcgctatgg ttcagataaa taatccgctc aataacagtt
    28021 ttttcagggt cgattttggg gattttatcg ccgacttgag tgatggggat aatgttgccg
    28081 gaaacgaacc gcccctgttt gtcggtgata attttgaaaa tcggcgcaat gccgctgatg
    28141 ccgtaggtgg caaagttgcc gccgctgtaa gagatgaagc ggtcgtggta aagttcgacg
    28201 gcgcgagtaa cgtgcgcccc ctgcccgaat acgacatccg cgccggaatc gacggcgagc
    28261 cgcgcaaact cgacgacgtt gcccctgttt tccccataga agatttcggt atcgaacggc
    28321 aggtgttcag cctgtttccc ttccgcgccg ccgtggaaca tcacaatgac gatgtcggtt
    28381 ttttgtttgg ttttccgaat cagttttttg aatttggcgt aatcgttcag tttgacggcg
    28441 gcaaggttgg gggcgaagga gacgaagccg tatcttacgc cgtttttctt caggatggcg
    28501 gtttcaaacc tgttttcgat gcccgaatat ttgatgttca attcgtcaag gttgcctgcc
    28561 gttgccgtga tgccttgcgc gccgaagccg ttgctgtggt tgtttgcgag gctgaggtag
    28621 tcgaatcccg cgtcggcaag gtattgcccg tatgcggagg gcgttcggaa tgcatagcat
    28681 attttggtgg tttgcacatt ttttcggcgt accgccttcg tcaaacagcg taccttatgc
    28741 cgccgacccc gatgatggaa acggtatctg ctgcctttcc gtcaggcgta tcctgtccgt
    28801 ccgcatcgcc ggtgtttgaa acgggcgcgg ggtttgaacc gtcaatcggc agcacggggg
    28861 attccgttcc ggcaggatgc gccgaggctt gaccccggcg gttttcggcg cggaaacagc
    28921 ataaggggcg gcaggcggct cttttacata ctcgagcgcg gtaaagccgc tgccgataac
    28981 gagggccaaa attgcggaaa ataccgccat cgcccagata aatttgttaa taatcatacc
    29041 tttactgttc agacggcatt tgccgcacgt tttggggctt atctctcgat ttgcgatacg
    29101 tcgcgcaccg cgcctttgtc ggcggaagtc gccatcgcgc cgtaagccct taatgcggcg
    29161 gagacgtagc ggtcgcggtt ttccggtttc cacgctttgc tgccgcgagc ttccatttcg
    29221 gcacggcgtg cggcaagctc ttcatcggaa atgacaaggc ggatgctgcg tttggggatg
    29281 tcgatttcaa tcgtatcgcc ttcgtgtacc aaaccgattg cgccgccttc cgccgcttcg
    29341 ggcgaggcgt gtccgatgga caaacctgat gtgccgccgg agaagcgtcc gtcggttaag
    29401 agggcgcagg ctttgcccaa gcctttggat ttcaggtagg aggtcgggta tagcatttcc
    29461 tgcatacccg ggccgccttt cgggccttcg tagcggatga tgacgatgtt gccggcgacg
    29521 atttggttgc ccaaaatatc ttcgacggcg gcttcctggc tttcaaacac gcgcgcgcgg
    29581 ccggtgaatt tgaggatgct ctcgtccacg cctgcggttt ttaccacgca gccgcgctcg
    29641 gcgatgttgc cgaataagac cgccaaaccg ccgtcttgcg agtaggcgtg tgccacgtcg
    29701 cggatacagc ctttttcgcg gtcaaggtcg agggttttcc aaatacggtt ttgcgagaac
    29761 gcctgagtgg tgcgcacgcc gccgggagcc gctttgaaac gttcgatggc gcgggtgttt
    29821 tcgggattgg tgacgtccca tttttcaatc gcgtctttca gtgtcggcgc gtggatcgtg
    29881 tacacgtcgg tgtgcagttt gcccgctttg tcaagttctt tcagaatggc gaagatgccg
    29941 ccggcgcggt gcacgtcttc catatagtag tcgtggttgt tgggcgcggt tttgcagatg
    30001 cagggcacga cgcggcttaa gcggtcgatg tctgccattt tgaaatccac gcccgcttcg
    30061 ttggcgacgg cgagcaagtg caggatggta ttggtgctgc ctcccatcgc aatatccatc
    30121 gtcatggcgt tttcaaacgc ttttttggtg gcaatgctgc gcggcagcac ggtttcgtcg
    30181 tcttgctcgt aatagcgttt ggtgatttcg acaatcatac gccctgcttc gaggaacaat
    30241 tctttgcggc ctgcgtgggt cgccaaatac gaaccgttgc cgggcaggga cagaccgagc
    30301 gcttcggtca ggcagttcat cgagtttgcc gtaaacatac ccgaacacga gccgcaggtc
    30361 gggcaggcgt tttgttcgac ttcttcgact tgccggttgc tgacattgtc gtccgccgat
    30421 tcgatcatgg cgtcaatcaa gtccaaacgg cgttcgggtt ggatgtttgc cacgccgatg
    30481 accttgcccg cttccatcgg gccgccggag acgaagatgg tggggatgtt caggcgcatg
    30541 gcggcaatca gcattcccgg ggtgattttg tcgcagttgg aaatgcacac cagcgcgtcg
    30601 gcgcagtggg cgttgaccat atattcgatg gagtcggcaa tcaaatcgcg gctgggcagg
    30661 gagtacaaca taccgctgtg ccccatggcg atgccgtcgt cgatggcgat ggtgttgaat
    30721 tctttggcaa ttgcacctgc tttttcaatc tcgcgggcaa ccagctggcc catattgtgc
    30781 aggtggacat ggccgggcac gaattgggtg aaggagttgg caacggcgat gatgggcttg
    30841 ccgaagtcgg tttccatcac gccggtggcg cgccacaatg cgcgcgcgcc cgccatattg
    30901 cggccgtggg tggaggtttt ggagcggtat tctggcatag tgtgtttcct tgtgcctata
    30961 ccgtctgaaa ggcagggctg tttcagacgg catcgggtac ggttttttag agtgggaaaa
    31021 gggggtattt tataccaagt atcggaattt tgcggaatgg aaacgtgtgc ggcaaaaaag
    31081 aacatccccg caggaatgcg ggggacgggt tcaggcgcgc gcaatcggga cggctttgga
    31141 tgcgtcccaa aaatcgacgg gcgcatccaa cacgggtttg acgatgcccg tccgtatggc
    31201 gaaatcgccg aaaccttcgt cggtatcgcg tcccgccgcc catttgccga tcaggtcttc
    31261 caattcggaa aggatttccg gcagggtgat gttttctttg tagaggcggg ggatgcgtac
    31321 gccttcgcgg tcgccgccga tgtggaggtt gtagcgtccc acggctttgc cgaccagtcc
    31381 gatttccgcc aacatcgccc gtccgcagcc gttcgggcag ccggtaatgc gggtaacgat
    31441 gtagtcgtcc gacgcgccgt gttccgccat aatcttatcc agctcgccga tgaagtccgg
    31501 caacacgcgt tcggcttccg ccattgccag cgggcaggtc gggaaggaaa cgcacgacat
    31561 cgcattttcg cgcagcttgc tgacaccgtc gcgtatcagc ccgtatgagc gggcgagctg
    31621 ttcgatccgg gctttgcctc cttcgggcac atttgccacg atgaggtttt ggttggcggt
    31681 gatgcggaag ccgcctttgt ggattttggc gatttccaac acgccggtca gaagctgttt
    31741 cccgccttcg tcaaccaaac gcccgctttc gatgaaaagg gtcaaatgcc agttgccgtc
    31801 tatgcctttc acccggccga tgcggtcgcc gcgcccggta aacttgaacg gccgtatggg
    31861 tttgaacggc atacccatac ggcgttcgac ttccgtgcgg aagttgtcca agcccatatt
    31921 ttgaatggtg tagcgggtgc ggtcgttttt gcggtggctg cggttgccga agttgcgctg
    31981 cgtggttacc accgcttcgg cggccttcag tgcgtgttcc ggaggcacga aacccagttc
    32041 cagtgaaatg ttcggatagg ttttggtgtt gccgttgccg tgttccgtcg aaagcccgcc
    32101 gcccgccaaa acattgaagc cggcaagctg tccgttgccg tctgaaacgg cgacgaaatc
    32161 caaatcgtta ccgtagcagt ccacatcgtt caagggcggg atgacgactg cggttttgaa
    32221 ttttcgcggc agataggttt tgcccaaaat cggctcgcct tcttgaagga agtcgtcgga
    32281 actttgaact tttttgccgt ccacccacat atccagataa ccgcgcgtgc gcggcagcag
    32341 gtgttcggaa atctttttcg cgtattcgta agcctgccgg tgcagctcgg actcgatcgg
    32401 gttggaggtg caaagcacat tgcggttcat atccgccgcc gtagcgatgg aatccaaacc
    32461 caggctgtgc aagaggcggg gcatcgtctg caacttggtt ttcggcacgc cgtgaaattg
    32521 gaaggtttgc cggttggtta gccggatgga gcggtaatga ctcttttccc gggcgaattt
    32581 gtccagttct atccaccggg acggtttgat gatcccgccg gcagccggca gcgcaaaagc
    32641 ataaatttca agggctcgag ttttgcctcg gtgcggatgt cgcggccatc ctgctcatac
    32701 atactgtgga agcggatgag ttggaagttg tcgcctttga agccgcccgt gagcgggtct
    32761 ttcaaatcgt ccaaaatcgt gccgcgtaaa aaattgcttt cggttttcag gcgttcgttg
    32821 tcggatagcg gcttttcttg ccacgccaaa ccttttgtct tggcctgtac ggtcattttg
    32881 tgttcctccc gattatattt aatcaataaa catcacgctg atagcgtttt tcttcgcgca
    32941 gcatatccaa atatccttct gcgccgtctt cgtccgaatg ccctgccccg ataatcacat
    33001 ccagcaaggc ggcttccact tcttttgcca tttttgccgc atcgccgcac acatagatat
    33061 gcgcgccttc ctgcagccat tgccaaagtc cttccgcctg ttcgcggatt ttgtcctgca
    33121 catagatttt ttcttcctga tcgcgcgacc aggcgaagtc atatctgtgc aggaagccgt
    33181 ctttggcaaa ctgctgccat tcggtctgat agaggaagtc ggcggcaaaa tgcggattgc
    33241 cgaaaatcag ccagtttctg ccttccgcat tttctgcggc acgttgttgg acgaaggcgc
    33301 ggaacggtgc gacgccggta ccggagccga tcatcacaat cggcttgcgg ctgtcttcgg
    33361 gcagcctgaa gccgtcgttg cgttccgcaa acacgcgcac cgtgccgtcc tcttccagcc
    33421 ggtcggcaaa gaaacccgat gcgccgcccg ccctggcgcg cccttcgtgt tcgaaacgca
    33481 ctgcgccgac ggtcaggtgc acttcgtccc ccgcttccgc ctgcgacgag gaaatcgaat
    33541 acaggcgcgg cgcaagcggg cgcagcaggc cggcgaattg ttccgccgtc agttttgccg
    33601 ggaagcggtg cagcacaccg gcaatcggcg tgctttgcac aaagccttgc aaaacggcgt
    33661 tgtcggcagc aatacggtcg agttcgtcat tatcggcgaa cgtggcatag cctttgacaa
    33721 aggcgggggt gttttgcgtg agttcgaaat gggataacag tgcggaggca accggcaggg
    33781 tttttccgcc cgcctgtatt tccgttgccg gattgatgcc gagcaggtct aggatttccc
    33841 cgaccagtgc cggatcgttg tcaaaccaaa cgccgagcgc gtcgcccggg aggtagtgca
    33901 aatccgaacc gctcaaatcg atttcgatgt ggcgcacgtc tttatcggat tggcgggcgg
    33961 tgattttctg attggccagc agggcggcgg gaaaggggtc tgccttgcag tacctgccct
    34021 ccgatgccgt ctgaaggccg gcggggggac gttgcctgcg gcgcgggcgt tgcccggttt
    34081 tttgcggctt cttcttttaa gcgcgcgaca atgcggcctg tccatccgtc tgcggcggcg
    34141 gcaaagtcca aatccgcatc aacgcgttcg agcaggcgtt ttgcgcccaa ttcttcaaaa
    34201 cgtttgtcga aatctttgcc cgcccggcag aaattcggat aggagctgtc gcccaaaccc
    34261 agtacggcaa attggagttt gtccaatttc ggggcttttt tgcagttcag cagtttgtgc
    34321 agcacgacgg cttcttccgg cggttcgcct tcgccctggg tggacgtaac cagcagcagg
    34381 cggcgttcgc cggcgatgtt tttcgccttg tagcctttca gttcggcgcg cctgacttgg
    34441 atgccggcgg ctttcaggct gtccgccgct ttgtcggcaa cggatttcgc attgccggtt
    34501 tgcgaggcgg aaaggacggt tacggaaaaa ggttctgccg ccggcaatgc cgtctgaagc
    34561 gcgggcagcc ctgcggatgc cccgtttcct gcttttgccc aagcgtagcc ggacagcccc
    34621 gacacaggag ctgcgtgatt tcgggcggca taggcggtaa tggcggattt gtgttctgca
    34681 tatcgtgttc actcataaaa tcatacctgc cgcaacagta ccgtatgtcg cttcgtctat
    34741 caggataaac gaaccggcgg cggtgttttc cgcataaggc gttgccgtaa cgggtttttg
    34801 aaggttgatg cggactttgg cgatgtcgtt catctttaag gattccgcgc cggcctcttg
    34861 ttccagcgtg cggacatcca aaacgctttc aatttccccg acttttgccg gcacggtttg
    34921 cgtgccgtgc ttgagcaggt atttgcgcgc ggtgttgagc gggcgttcgt caaaccagca
    34981 aagcgtggct tccggatgtt tttgcggggc gagcgggaaa tttttatcga caaaaagatc
    35041 gccgcgcgaa acatcgatgt cgcggtccag ccggatggtt gccgcctcgc cggcaaaagc
    35101 ctgcgccact tcccctttcg gcgcgatgat ttcggacact tcggcggtca gcccgttcgg
    35161 ttcgatgcgg acggtttgcc cgacggtgac cgaaccgcgt tcgatgcgcc cctgatagcc
    35221 tcggaaaccg tcggccttgt cggcatcttg gcggacgacc agttggacgg ggaaataaaa
    35281 accggcatcg cgccaccttc ctgaaatttc cgccatatcg cccaaagacg gcggcagcaa
    35341 ggcttccagc ctttcacgca gcaggcgggc gaggacgggc gcgctgccgg agctggaaac
    35401 ggcaatctga acagggtcgc ggtcgataac cgacgggaag atgaagctgc aatggtcacg
    35461 gtcgcccacc acgttgaccg gcttttggca gctttcggca agatggaaaa cgcgccggtt
    35521 gagggcttgg tcgctgcttg ccgcaatgat gaggaaaacc gtgcggatgt gttcggcacg
    35581 aaattctttg gcaagccaca ggattttgtt ttccgccgcc aacgcggaga gttcggcatt
    35641 caggtgtttt gcggcaaccc cgacctctgc gcccgccttc ggcagcaggc tgattttgcg
    35701 tgcggcgacc gcgccgccgc ctacgaccaa tacggggcgg ccggagaggt tggcgaaaat
    35761 gggaaaataa ttcactggct gactcctttg ctgtttgccc gcaccttgtt tccgatacgg
    35821 tgcgtcgcgg catttttgtc ggaatgcggg tcattttaaa taataaaaag gcatttgtta
    35881 gaagctgaaa gctatatggg gggcggctgc ggatgcggcg gttttcc
//
LOCUS       DAWTHD010000006        35863 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWTHD010000006 DAWTHD010000000
VERSION     DAWTHD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35863
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.006"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            113..505
                     /locus_tag="WM704_000217"
     CDS             113..505
                     /locus_tag="WM704_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339733.1"
                     /translation="MQKKRHSRRHLFIPTSSKHISGLNIHSIPVLKGTGSHSVKKSGM
                     SSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKIKKSYPQKIPHINIII
                     VLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            585..2033
                     /gene="gnd"
                     /locus_tag="WM704_000218"
     CDS             585..2033
                     /gene="gnd"
                     /locus_tag="WM704_000218"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFA9339734.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2096..3367
                     /gene="waaA"
                     /locus_tag="WM704_000219"
     CDS             2096..3367
                     /gene="waaA"
                     /locus_tag="WM704_000219"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFA9339735.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3408..3857
                     /locus_tag="WM704_000220"
     CDS             3408..3857
                     /locus_tag="WM704_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFA9339736.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            3890..4864
                     /locus_tag="WM704_000221"
     CDS             3890..4864
                     /locus_tag="WM704_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFA9339737.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            4933..5008
                     /locus_tag="WM704_000222"
     tRNA            4933..5008
                     /locus_tag="WM704_000222"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4966..4968,aa:Lys,seq:ttt)
     gene            5229..6482
                     /gene="murA"
                     /locus_tag="WM704_000223"
     CDS             5229..6482
                     /gene="murA"
                     /locus_tag="WM704_000223"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFA9339738.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            6563..7756
                     /locus_tag="WM704_000224"
     CDS             6563..7756
                     /locus_tag="WM704_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFA9339739.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGAAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(7808..8056)
                     /locus_tag="WM704_000225"
     CDS             complement(7808..8056)
                     /locus_tag="WM704_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HFA9339740.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8149..9066)
                     /gene="ftsX"
                     /locus_tag="WM704_000226"
     CDS             complement(8149..9066)
                     /gene="ftsX"
                     /locus_tag="WM704_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HFA9339741.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9063..9713)
                     /locus_tag="WM704_000227"
     CDS             complement(9063..9713)
                     /locus_tag="WM704_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFA9339742.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9907..10389)
                     /locus_tag="WM704_000228"
     CDS             complement(9907..10389)
                     /locus_tag="WM704_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFA9339743.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10513..10866
                     /gene="arsC"
                     /locus_tag="WM704_000229"
     CDS             10513..10866
                     /gene="arsC"
                     /locus_tag="WM704_000229"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFA9339744.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10863..11519
                     /locus_tag="WM704_000230"
     CDS             10863..11519
                     /locus_tag="WM704_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFA9339745.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11635..13029
                     /locus_tag="WM704_000231"
     CDS             11635..13029
                     /locus_tag="WM704_000231"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFA9339746.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13050..13445
                     /locus_tag="WM704_000232"
     CDS             13050..13445
                     /locus_tag="WM704_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339747.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            13449..13940
                     /locus_tag="WM704_000233"
     CDS             13449..13940
                     /locus_tag="WM704_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9339748.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14216..16810)
                     /gene="mutS"
                     /locus_tag="WM704_000234"
     CDS             complement(14216..16810)
                     /gene="mutS"
                     /locus_tag="WM704_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFA9339749.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17241..18245
                     /gene="gap"
                     /locus_tag="WM704_000235"
     CDS             17241..18245
                     /gene="gap"
                     /locus_tag="WM704_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFA9339750.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18397..19077)
                     /gene="tsaA"
                     /locus_tag="WM704_000236"
     CDS             complement(18397..19077)
                     /gene="tsaA"
                     /locus_tag="WM704_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFA9339751.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            19537..20172
                     /locus_tag="WM704_000237"
     CDS             19537..20172
                     /locus_tag="WM704_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFA9339752.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVGNPVDMLKPTGLSDADLTWVAHAMVGTRGYELLDGLPSVKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
     gene            20209..21177
                     /gene="waaC"
                     /locus_tag="WM704_000238"
     CDS             20209..21177
                     /gene="waaC"
                     /locus_tag="WM704_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFA9339753.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21403..22152
                     /locus_tag="WM704_000239"
     CDS             21403..22152
                     /locus_tag="WM704_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFA9339754.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQTGVKVASVAELVEKLKNEAKVI"
     gene            22163..23098
                     /locus_tag="WM704_000240"
     CDS             22163..23098
                     /locus_tag="WM704_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFA9339755.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23161..23772
                     /locus_tag="WM704_000241"
     CDS             23161..23772
                     /locus_tag="WM704_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFA9339756.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLVNYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(23824..24162)
                     /locus_tag="WM704_000242"
     CDS             complement(23824..24162)
                     /locus_tag="WM704_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFA9339757.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            24299..25570
                     /gene="purD"
                     /locus_tag="WM704_000243"
     CDS             24299..25570
                     /gene="purD"
                     /locus_tag="WM704_000243"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFA9339758.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25604..26206
                     /locus_tag="WM704_000244"
     CDS             25604..26206
                     /locus_tag="WM704_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFA9339759.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPSENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26221..26736
                     /locus_tag="WM704_000245"
     CDS             26221..26736
                     /locus_tag="WM704_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339760.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(26888..27328)
                     /locus_tag="WM704_000246"
     CDS             complement(26888..27328)
                     /locus_tag="WM704_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339761.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(27560..27739)
                     /locus_tag="WM704_000247"
     CDS             complement(27560..27739)
                     /locus_tag="WM704_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFA9339762.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(27745..28332)
                     /locus_tag="WM704_000248"
     CDS             complement(27745..28332)
                     /locus_tag="WM704_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFA9339763.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(28354..29100)
                     /locus_tag="WM704_000249"
     CDS             complement(28354..29100)
                     /locus_tag="WM704_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFA9339764.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(29090..29932)
                     /locus_tag="WM704_000250"
     CDS             complement(29090..29932)
                     /locus_tag="WM704_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HFA9339765.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(30050..30358)
                     /locus_tag="WM704_000251"
     CDS             complement(30050..30358)
                     /locus_tag="WM704_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339766.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(30432..30881)
                     /locus_tag="WM704_000252"
     CDS             complement(30432..30881)
                     /locus_tag="WM704_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFA9339767.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPKCRLKQD"
     gene            31222..32115
                     /locus_tag="WM704_000253"
     CDS             31222..32115
                     /locus_tag="WM704_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFA9339768.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            32218..33321
                     /gene="prfB"
                     /locus_tag="WM704_000254"
     CDS             32218..33321
                     /gene="prfB"
                     /locus_tag="WM704_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFA9339769.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            33400..33759
                     /gene="rnpB"
                     /locus_tag="WM704_000255"
     ncRNA           33400..33759
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WM704_000255"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(34088..35545)
                     /locus_tag="WM704_000256"
     CDS             complement(34088..35545)
                     /locus_tag="WM704_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9339770.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSRILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT         8687 a         8224 c        10093 g         8859 t
ORIGIN      
        1 atgcttatac acaaccagcc aaaagccatc ttattgaaat atcataagat ttactcaaaa
       61 atcttatttt ctccaccact tatgcacaaa ctcggataca cactctgcca atatgcagaa
      121 aaaaagacat tcgcgccgac accttttcat ccccacaagt tccaaacaca tttccggctt
      181 aaatatccac agcattcccg ttcttaaagg caccggcagc cattcggtaa agaaaagcgg
      241 gatgtcgtcc gcacaacaag caaactatcc acaaaaccta ttccccacga aaaacaatcc
      301 acaggtatgc acaaaaggga atcctcctta ccgacaccct tttaaaacgg caattcagat
      361 gattttaaaa ataaaaaaaa gttacccaca gaaaattccc catatcaata taatcatcgt
      421 tttattattt aaaccttata ttttatttat aacccttcag ggaaacggca cggcaaagcg
      481 taaaggcatc ttttcaaaaa attaaaaagt taaggattgt taaacaagtg taaatcaggt
      541 agagttgcag ccgataaatt aaatctataa cggagcttcc tgcaatgaaa ggcgatattg
      601 gcgtaatcgg tttggcggtt atgggtcaaa acctgatttt gaatatgaac gattgcggat
      661 ttaaggttgt cgcctacaac cggacaatcg gtaaagtgga cgaattttta aacggtgcgg
      721 caaaagagac gggcattatc ggcgcgtatt ccctgcaaga cttggtcgat aagctggcaa
      781 aaccgagaaa aatcatgatg atggttcgcg caggttcggt ggttgacgac tttgtcgaac
      841 aactgcttcc gctgttggaa gaaggcgaca tcattatcga cggcggcaat gccaattacc
      901 ccgacacgac acggcggaca cattaccttg ccggaaaagg gattttgttt gtcggcgcgg
      961 gggtatccgg cggcgaagaa ggcgcgcggc gcgggccgtc catcatgccg ggcggggata
     1021 agcgcgcttg ggaagcggtc aagccgattt tccaggcgat tgccgccaaa acgccgcagg
     1081 gcgaaccgtg ttgcgactgg gtcggcaagg atggcgcggg acattttgtc aaaatggtgc
     1141 acaacggcat cgaatacggc gatatgcagc tgatttgcga agcgtaccag tttatgaaag
     1201 acggtttggg gctgtcctac gacgaaatgc accgcgtgtt tgccgagtgg aacaaaaccg
     1261 agctggattc ttatctgatt gaaatcacag cggcaatttt gggctataag gacgaaggcg
     1321 gcgaaccgct ggccgagaaa atcctcgata cggcagggca aaaaggcacg ggcaaatgga
     1381 cgggcatcaa tgccttggat ttgggcattc cgctgacgct gatttccgaa gccgtattcg
     1441 cccgctgtgt ttcgtcgttc aaagaacagc gcgtgcagac cggcaagctg ttcgcccgaa
     1501 ccgccacgcc cgtcgaaggc ggcaaacaag aatgggtcga ggcgttgaga caggctttgc
     1561 ttgcctccaa aatcatttcc tacgcacaag gctttatgtt gatccgcgaa gcgggcgaaa
     1621 gttacggctg ggatttggac tacggcaaca ctgcgctgct gtggcgcgaa ggctgcatca
     1681 tccgcagcgc gtttttgggc aatatccgcg acgcgtatga ggccaatccc gatttggtgt
     1741 tcttgggtgc ggatccgtat ttcaaaaata ttttggaaaa ctgcctgccg gcatggcgca
     1801 aggtggttgc caaggcggtc gaatgcggca tccctatgcc ctgcatggct tcggcgatta
     1861 cgttcttgga cggctatacg accgaacgcc tgcctgccaa cctcttgcag gcacagcgcg
     1921 actacttcgg cgcgcacact tacgagcgca ccgacaaacc gcgcggcgaa tttttccata
     1981 ccaactggac gggcaagggc ggagataccg cttcgacaac ctacgatatt tgattccgtt
     2041 gccgaaatgc cgtccgaacc gttcggacgg catttgtttt gcaggaaata gaaagatgtt
     2101 ccaatggctt tatgatgtat tgtggctgct tgcgccgata tggatacggc gttatttgga
     2161 caaacgctcc ggaagtgccc cggcataccg ggcgcatagg gacgagcgtt tcggcaagcc
     2221 gcatccgaat cccgttaccg gcgcggtttg gatacacgcc gtttcggtcg gagaaacgcg
     2281 tgccgcccag cccttgatac gcgagttgcg gcggcgtttt cccgatgcgc cgctgctgat
     2341 gacgcagatg accccgacgg gacgggaaac cgcgcaagtt ctgtttcccg atgcgcaatg
     2401 ccgctatctt ccgtatgaca aaaaaacgtg ggtacggcag tttttgcgcg aacaccgccc
     2461 gatgttcggc attttgatgg aaaccgaaat ctggcccaat ctgatgaagg aatgccggcg
     2521 agcgggtgtg ccgctgtttt tggcgaatgc gcggctgtcg gaaaaatcgc tgaacggtta
     2581 tctgaaagtc cgccgcctga tccgtcccgc agccgcttcg ctgacgggct gtctggcgca
     2641 gacggaggcg gatgctgcgc ggttggcgaa attgggcgcg gcatccgtgc aggtgtgcgg
     2701 caataccaaa tacgacctga tgccgtctga agacatgaaa acgctggcgg ggcagtttga
     2761 aaaacgcatc ggaggccggc cggttgccgt gtgcggcagc acgcgcgttt atcggggcga
     2821 agacgaggcg gaaaaactgc tggcggcgtg gcaacaatat cgcggcgatg ccctgctggc
     2881 cgtcgtgccg cgccatcccg agcattttca gacgacattt gaaacggcga aacgcttcgg
     2941 gtttaaggtt cagcggcgca gcgacggttt gccggtcgaa cccgatacgc aggtgtggat
     3001 aggcgacagt atgggcgagc tgtatgcgta ttacctgtgc gccgatgtcg cttttgtcgg
     3061 cggcagtctg gtcggttcgg gctgtcagaa catcatcgaa ccgctttcct gcggcgttac
     3121 gacgatattc ggcttttcga cctacaattt ttccgaagcc tgccggcacg ccttggcatc
     3181 gggtgcggcg gttcaagtcg aatcggcgga cgcgtggcgt gaagccgttg aaaaaaactt
     3241 atctggcgag gggggcggaa tgcagatgca ggcgcgcgtg gacggcttta tcgcacaaca
     3301 tcgcggagcg ggcgcgagaa tcgccgaggc ggtgcgggaa gcggtatgcg gacatcgggg
     3361 gcgatagtga tacaatccgt atccgagttt tccggttgga gcatcgtatg agtatttatg
     3421 ccgtcgcgca catcatccac ctgtattgcg ccaccgcctt tgtcggcggc gtgttttttg
     3481 aagtgctggt tttgtccgtc ctgcatacgg gacgggtgtc gcgcgaggcg cggcgcgaag
     3541 tggaaaaggc aatgtcttac cgcgccgtca gggtgatgcc gtttgcggtc ggactgctgt
     3601 tcgccagcgg catcgtgatg gcggcaaacc gctaccttcc tatatcgggc gaaccgtttg
     3661 ccacttcctt cggtacgatg ctgacgctga aaatcctgtt ggcgttcagc gtgttggcgc
     3721 acttcgccat cgccgtcgtc aaaatggcgc gttccacgct gacggtcggc tggtcgaaat
     3781 acatacacgc cgtcgtcttt acccatatgc tgctgattgt ctttttggca aaagcgatgt
     3841 tttatatcag ctggtaacac aactacattt caaatcagga gattgaacca tgacagaata
     3901 tcccggcatc gggtcgccgt tgttttacgg cgtttttttt gcggcagtgc tggtcatgat
     3961 tgccttggat atgttttcgc tgaagaaaaa cggcagccat aaagtcggca tcaaagaaac
     4021 cttggcatgg agcggcttgt gggttgccgt atcctgcctg ttcgcaggct ggctgtattt
     4081 cgaactcgcc ggcaatcccg gctacggcgc ggcagccgca aaagaaaaag tattggaatt
     4141 ctttaccggc tacattttgg aaaaatcgtt ggcagtcgac aatattttcg tgttcctgat
     4201 gatattcggc tacttcaaag tcgcaccgca gtttcaacac cgcgtgctgc tgtacggcgt
     4261 attgggcgca ttggtattgc gcaccgtcat gattttcgtc ggcgcggcac tggttcggca
     4321 gttcgagtgg attctgtatc tgttcggcgc gttcctgctc tataccggca tacacatgat
     4381 gaagcccgaa ggagatgagg aagaggattt ggcaaacagc aggctgctga atgccgtcaa
     4441 gaaagtcgtt ccggtcggca cggcgtttca cggcgagaaa ttttttaccg tcgagaatgg
     4501 caaaaaaatc gccacgccgc tgtttttggt gctgattatg attgaattga gcgatgtcgt
     4561 ttttgccgtg gacagtatcc ccgccgtctt tgccgttacc accgatccgt ttatcgtgct
     4621 gacttccaat attttcgcta ttttgggttt gcgggcgatg tatttcctgc tggcggatgt
     4681 ggcggaacgc tttatcttcc tgaaatacgg cttggcattc gtgttgggtt ttatcggtgt
     4741 gaaaatgctg gtgatgcatt gggtacacat cccgatttcc gtttcgctgt cggtcgtgtt
     4801 cggcgcgttg ggtgcatcga tactgacctc gttaatttat acgaaaaaac agcctgataa
     4861 ataaaacgtc gtctgaacgc cgacaaaatg gttgacagca tcaggcttga tgtataaact
     4921 ttcaaatttt tcgggtcgtt agctcagtcg gtagagcagc ggacttttaa tccgttggtc
     4981 gagcgttcga atcgctcacg acccaccagg taaagcaaaa aagctgagtt ttcaaaactc
     5041 ggcttttttt tgttttccga caatccttgt tcggaaagcg cggcatttcg tcaaattctt
     5101 tggtgcggct tccgtatttt tcccgaaaat cccttataat tccgcctctg cagccttcat
     5161 cgggtgatgc tttatgcatt gtcccgagta ttgaaatatt agcaagtacc caccaggaaa
     5221 gaacaaacgt ggacaaactg aaaatctccg caaacggccc gctcaacggg gaaataacgg
     5281 tctcgggcgc gaaaaacgcg gcattgccgc tgatgtgcgc gggtttgctg acatcgggta
     5341 cgttgcgcct gaaaaacgtc cctatgctgg cagatgtcgc aaccacgcaa aagctgcttc
     5401 agggcatggg cgcgcgcgtt ctgaccgaca atatcagcga atttgaaatc aacggcggta
     5461 cggtcaacaa tacctgcgcc ccttacgaat tggttcggac gatgcgcgct tcgattttgg
     5521 tgcttggccc gacgctggcg cgtttcggcg aggcgcaagt cagcctgccg ggcggctgcg
     5581 ccatcggttc gcgccccgtc aatcagcatt tgaaaggctt ggaagcgatg ggggcggaaa
     5641 tcgccatcga acacggttac gtcaaagcca aaggcaaact caaaggtgcg cgcgtggcga
     5701 tggatgtcgt taccgtcggc ggtacggaaa acctgctgat ggcggcgacg ctggcggaag
     5761 gtacgacggt tttggaaaac tgcgccatcg agcctgaagt ggtcgatttg gcggaatgcc
     5821 tggtcaaaat gggcgcgaaa atcagcggca tcggaacatc gaccatgatt gtggaaggtg
     5881 cgggcgagct gtacggctgc gaacacagcg tcgtccccga ccggattgaa gcggggacgt
     5941 tcctgtgtgc ggtggcgata accggcggca gggtggtttt gcggaatgcc gccccgaaaa
     6001 cgatggaagt ggtgttggac aaactggtcg aggcaggtgc ggtgattgaa gcgggcgacg
     6061 attggatcgc catcgatatg cggcagcgtc cgaaggcggt ggacatccgc acggtcgtcc
     6121 atcccggctt ccccacggat atgcaggcgc agtttatggc attgaatgcc gtggcagagg
     6181 gaagctgccg cgtggtggaa acgatttttg aaaaccgctt tatgcacgtt ccggagttga
     6241 accggatggg ggcgaacatt acaaccgagg gcaatacggc atttgtgcag ggtgtggaac
     6301 ggctttccgg cgcggtcgtc aaggcgacgg atttgcgcgc gtccgccagc ctcgttatcg
     6361 ccggtttggc ggcgcgcggc gaaaccgtgg tcgaacggat ttaccacttg gatcgcggtt
     6421 atgaaaatat tgaaaaaaaa ctcggcagcg tcggtgcgaa catcgagcgc gtatccggct
     6481 gatcgggttg cgatgccgtc tgaaggcttc aggcggcatc aattgtttct gtatcgcaaa
     6541 cactgattga aaaggactga ttatggcatt tttaaaactg accgaacaaa acgtgcgggg
     6601 caaaaccgtc ctcatccgcg ccgatatgaa cgtgccgttc aaaggcggca aaatcagcga
     6661 cgacacccgt atccgcgcct cgctcgcgtc cgtcaaatac tgcttggaca acggcgcgtc
     6721 cgttattgtg atgacccact tgggccgccc gaccgaaggc gagttccacc ccgaagacga
     6781 cgttgcgccc gttgccgcgc acttgggcgg cctgttgggc aaagacgtga aagtattgaa
     6841 cgactggcgc gaaaacaaac cggctttgaa cgcgggcgat gtcgtgatgc tgcaaaacgt
     6901 gcgtatcaac aaaggcgaga agaaaaacga tttagaactg ggcaaagcct acgccgcctt
     6961 gtgcgacgtg ttcgtcaatg atgcgttcgg cgccgcacac cgcgcccaag cttcgaccga
     7021 agccgttgcc caagccgcgc ccgtcgcctg cgccggcgta ttgatggcgg gcgaactcga
     7081 cgctttgggc aaagccttga aacagcccgc gcgcccgatg gtggcgattg ttgccggcag
     7141 caaagtgtcc accaagctga ccatcctcga atcgttggcg gacaaagtcg atcagcttat
     7201 cgtcggcggc ggcatcgcca acacattcct gttggcggaa ggcaaagcca tcggtaaatc
     7261 gctggcggaa catgatttgg tggaagaatc caaaaaaatt atggcgaaaa tggcggccaa
     7321 aggcggttct gtgccgctgc cgaccgatgt cgtcgttgcc aaagcctttg ccgccgatgc
     7381 cgaagcggtg gtaaaagaca tcgccgacgt tgccgaagac gagatgattt tggacatcgg
     7441 cccgaaatcc gccgccgcgc ttgccgagtt gcttaaagcc gccggtacgg tcgtttggaa
     7501 cgggccggtc ggcgtgttcg agttcgatca attcgcaggc ggcacgaaag cccttgccga
     7561 agccatcgcc caaagcaaag cgttctcgat tgcgggcggc ggcgacacgc tggcggcgat
     7621 tgccaaattc ggcgttaccg aacaaatcgg ctacatctcc accggcggcg gcgcgttcct
     7681 tgagttcttg gaaggcaaag agctgcctgc cgtagccgct ttggaaaaaa cgcggcgagt
     7741 aaacggtttg atataaaaaa atgccgtctg aaacgaaatg gtttcagacg gcatttttgt
     7801 gtggcggtta ttgtgctttg gcagcccatt ccgccggagt ggcggcaacc gaaatggaaa
     7861 gtgcgtgcag ttcgttgctt tccagttggg ctttgagtcc gtctttaatc aggcggtggc
     7921 gcgcgaggcg tgccttgcct tcaaattctg atgaaacgat gacggcgaaa aaatggtgtc
     7981 cgtcgccttc tacttcgatg tgttcgcatt ttgccacgcc tgcaatcagg gccttgactt
     8041 gttcgggtgt cagcatagtg gggagtcctt attggggaaa tgcgccatta tactggcaaa
     8101 tcgaaattcc gtctgaaaac ggcttcagac ggaatttttg acggtgtttt attttttggc
     8161 tttgaagcag agcaggtgtt gtgtggtggc aagccacgcg ccgaatacgc ccaacgcgat
     8221 gacgaagccg aacaccagcc cgagttcgcc gacgtagaag aaacgccagc cgatgttcaa
     8281 gccgtagggt ttgaagatgg catcgaccaa ggggtgcacg gcagagagca gccaaccgca
     8341 aagccctaag ctgacggcgg cggagaagat actctgccac atagcctgat aaaggaaggg
     8401 gcggcggata aacgacgcgg gcgcacccag gagtttggtg atttcgattt cttctttgcg
     8461 gctgaggatt tgcagtcgga tggtgttgtg tgcgacaagg acgaaagcca tacccagcgt
     8521 cagggaaaga aaccataaga ttttgcggat gaactcgttg atttggtaca gcgtttgcac
     8581 ccattcggta tccatagacg cggattcgac cataggcagc ttggtaatgt cccggtagat
     8641 tgcctgcatt tgggcgggcg tggttgccgg gtcgggggta acgataaaga catccggcag
     8701 ggggttgccg tcaagcatgg aaatcagatt ttggtcgagg ttggactgta attccgccaa
     8761 accgtcttcc ttgccgatga agcggatgtt gtcgagccgt ttatcgcgcg tcagcaggct
     8821 gcgtacggta tcgctgtcgc tttgtgcggc ggcggtttcc atatagacgg tgatttgcgg
     8881 cgactcgttg agtttgccca acacgctttg cccgctttgg atgcccagat acataaacag
     8941 cggcagggtc atcgcgacgg caagcattat gagggtaagc agtgtgccga agggttggcg
     9001 cagaagctgt ttgagtgcgg agcgcgcgga ttcgacgtgc agcgagaagt agtggatgat
     9061 gctcatgcga gtcgtccttt cgagaggcgc aggatgcggt gtccgtagtc cgccatcagg
     9121 gtttcgtcat gtgcggcgac gatgacggtg gttcccgctt cgtggaaggt tttgaacaat
     9181 tccataatat cgagcgcgta ggcacggtcg aggttggcgg agggttcgtc ggcaatcagc
     9241 aggccgggct ggtggacgac ggcgcgggcg atgcacaggc gttgttgttc accgcccgag
     9301 agggttacgg gatcgtccaa ttctcgtcct ttcaagccga ctttttcgat ggcgatgcgg
     9361 gcgcgttctt cggctttgcg cggcggatag ccgataatcc gcaggggcag gatgacgttt
     9421 tgcaggacat tgcggtcgtt gaggattttg tggtcttgga acacgatgcc gatgtgttgg
     9481 cgcataaagc cgatttggtt gtcggacaat gtgccgaggt cctgcccgtt aaacaggact
     9541 ttgcccatgc tcggcttggt gatgcccgaa atcagcttga ggacggtgga tttgcccgaa
     9601 ccggagtgtc ccgcgataaa aatcatctcg cctttgttga tttggaagct gacgtttttc
     9661 agggcttcaa aaccgccggg ataggttttg gaaacttgtt cgaaacggat cataggaggt
     9721 cctgtaagga agggcggcat atcgtttcgg gcggttgcgt ccgcagggat atgccgtctg
     9781 aacaataggt aagacagtac cgattataaa gattccgcct gtttttagat agtggataag
     9841 tttggcggat accgaaggcc gggcgggaaa atgccgtctg aagcggcttc agacggcatc
     9901 cggcgtttaa cggcattttg aatgggcgag tttgacggct tcggtcaggc ttttctcgtt
     9961 cacctctcct gtaatggtct gcctgtatcc gcatttcggg gcttcgacga cggtaaaggg
    10021 cagtacgccg acattgtttc cgtaggattt cataaagttt cggctgttcg ccccggtgta
    10081 acgccaaatc gggtagctga ccggggtctg cttgagaaag ttgccgatat tgtcggatgt
    10141 gtcgagcgcg atgccgacca tatcgacgct gccttttttc tgcgctttgt accatttgga
    10201 catggcgggc atctcttttc gacacggacc gcaccaagtc gcccataggt tggcgatgcg
    10261 gacgggggct ttgagcgatt gcaggttttg cggggtattg tctttccatc cggccagttc
    10321 gtctgccgag gcggtatgtg cggcaaatgt ggcggcaagg gcgattgcgg cgagattcaa
    10381 gcgtttcatc gttttgtcct ttgtatgtcg attcgggtat tgtaaaccgt ttggtacggt
    10441 tggaggcggg gtattgagta aaaccctgcg taagccgata ataaggggat tgtcgagaag
    10501 tagggaaatg tcatgtctga aatcaaaatt ttccacaatc cgcgttgcag caaatcgcgt
    10561 gccgccgtgt ccctattgga agaacgcggc attgctgccg aagcggtcaa atatttggat
    10621 acgccgcccg acttgtccga attgaaggat attttcaaca aattgggctt ggaatcggcg
    10681 cgcgggatga tgcgcgtgaa agatgatttg tacaaggaat tgggtttgga caaccccgat
    10741 ttggacaatg acgcgctgct gcgtgccatc gccgatcatc ccgccctgtt ggagcgtccg
    10801 attgttttgg caaacggtaa ggcggctgtc ggcagaccgt tggaaaatat cgaagcggta
    10861 ttgtgaacaa acggctgttt tgcagccgca acggcttgcg ctattacctg ttgggcggtt
    10921 tctgtttgag tgtttttccg cttttgctgg tgtttgcctc atccgtttgg gcggtttatc
    10981 ggacgggcgg gcaggttttg ccgccgtatg tccgggcgga tgccgcgctg gtgttgggcg
    11041 cagccgcgtg ggacaaacgc ccttccccgg tttttcggga acgcatcaac cacgccatcg
    11101 cgctttacca aagccgcagg gtaggcaaaa tcgtgtttac gggagggcga accaaaaaag
    11161 gctatatgac cgaagccgag gtcgggcggc ggtacgcgct caaacagggg gttccggcgc
    11221 gcaacatcct gtttgaaaac acttcgcgca acacttatga aaacctgaac aacatccgtc
    11281 cggttttgcg tgccaacggt attgccagcg tggtgattgt cagcgatccc taccatttgg
    11341 cgcgggcggc ggaaatggcg gaggatttgg gtgtccgcgt ttatatgtct gccacgccga
    11401 ccacgcggtt tgatgcggga aataagaaaa aaatctttat gctgcaagag gggtatgcgt
    11461 tatccctcta ccgtttggaa aaatggggca gccgcttttt gggatggctg tctgattgaa
    11521 gcggtagaaa atgccgtctg aaacttatcc gcaggcaggg ttcggacggc atttttgatg
    11581 cctaagcacg gtaaaaacag taaaataaac gctttcaaag atttcggagc cattatgacc
    11641 gtcaaaaccc gtttcgcccc cagccccacc ggttacctgc acatcggcgg cgttcgcacc
    11701 gccttgtttt cgtgggcgtt tgcccgccat cataaaggcg aatttttatt gcgtatcgaa
    11761 gacaccgact tggcacgttc caccgccgaa tccgtcaaca tcatccttga cggcatgaaa
    11821 tgggtcggtc tcgattacga caacgccgac aacgtggtgt accaaacccg ccgtttcgac
    11881 cgttataaag aagtcattgc cgaactcttg gcaaaaggcg atgcctacta ctgctattgc
    11941 agcaaagaag aattggaagc gatgcgcgag aaagccgaaa aagaaggcac ggcgacttac
    12001 gaccgccgct ggcggccgga agcaggcaaa accctgcccg aaattcctgc aggcgtgcaa
    12061 cccgtcgtcc gcttcaaaac gcctttggac ggcgttacca aatggacaga cttggtcaaa
    12121 ggcgaaatct ccatccccaa cgaagcccta gacgacctga ttattgcccg cgccgacggt
    12181 acgccgacct acaacttctg cgccgtggta gacgactacg acatgggcgt tacccacatt
    12241 atccgcgggg acgaccatgt gaacaacacc ccgaaacaaa tcaacatctt gaaagccatc
    12301 ggcgcgaacc tgcccgaata cgggcatctg ccgatgattc tcaacgaaca aggcaaaaaa
    12361 atctccaaac gcagcggcga taccgtcgcc attaccgatt tcggcgcaat gggcattctg
    12421 cccgaagcga tgctcaacta tttggcgcgt ttgggctggg cgcacggcga cgatgagttt
    12481 ttcacgacgg aacaattcat cgaatggttt gatttgaaag acgtttcccc gtccccaagc
    12541 cgtatggatt tgaaaaagct ctattggatc aacggcgaac acatcaaaat cacacccgac
    12601 ggcaaactta ccgaactcgt caaaccgcgc cttgcgctgc gcgatattca tgaaaccgaa
    12661 aaacctgcgt tggaagacgt gttggcactg gtcaaagacc gcgcccaaga tttgaacgcc
    12721 ttggcagacg aatgcctgta tttctacaaa aaacaagtcc ctgccgaagc cgatgttgcc
    12781 aaacactggg acgacgaagc cgccgcccgt atgctgcgct tcgccgaacg cctcgaaggg
    12841 ctggaagact ggaacgccaa agccattcac gacctcttca aacctttctg cgacgaagaa
    12901 ggcatcaaaa tgggcaaact cggtatgccc ctgcgccttg ctgtctgcgg tacggcaaaa
    12961 accccgagcg tcgatgccgt gttggcatta atcagcaaag aggaagtgtt gaaacgcatc
    13021 cgtgcttaag ttatccacag gtgcgggcaa tgccgtctga tgtcggaatg cggcttcaga
    13081 cggcattcag acggaagttg aacatgaaaa aaatattgta ttttctgatg tttgtttttt
    13141 ctacaagcgt atgggcaggg ggtgcggaag acaatttgct cagcatccaa tccggttacc
    13201 gtgctttact gcaaaagcaa aacaatctgg acggaaaaat catcgggatg cagtcggatt
    13261 tggaagatgc gcgccggcgt ttgcaggcgg ctcaggcaga catcgcccgt ttggaagcgg
    13321 aaattcctgc agcaatggcc caaaaagccc ggcaggctga agagatgagg caaatcggag
    13381 tgcgtttgga ccatgcttgg aatgcggttt acggcgcagg ggaacgaaag cgtcggggga
    13441 attgaccgat gaattccctc tttgtggaca acactgtttt tattacacgg ctgaaagccg
    13501 ggcatatcgg caggttggtt caggcgttgt ttgaggagtg gcacggattt gaaccgtggt
    13561 cttctgtgga taagattcgt gcctattacg gcagatgttt gaaggatgac gaattgccgc
    13621 tggcttttgc ggctgtggat gattccggaa ccctgttggg ttcggctgca gtcaagcggc
    13681 atgatatgga aaattttcca cagtatgaat attggttggg cgatgtcttt gttttacctg
    13741 aatatcgcgg aaaaggcatt ggcaggaggc tggtcgccca ctgcataggc gcagcgcgtt
    13801 cgctggggat aaagttcttg tatctttata cgcctgatgt gcaaatattt tatgaatcat
    13861 tcggctgggc ggttgtcggg cgacatttcc ataacgggga atgggttacg gttatgcgtt
    13921 tggatgtgga taaggtttaa gccgaatggg cagatgccgt ctgagctgtt cggacggcat
    13981 tttttatgtt tggaaatttc ctgtcagata tagtggatta acaaaaaccg gtacggcgtt
    14041 gccccgcccc ggctcaaagg gaacgattcc ctaaggtgcc caagcaccgg gcggatcggt
    14101 tccgtaccat ttgtactgcc tgcggcccgc cgccttgtcc tgatttttgt taatccgcca
    14161 taaagtttat ccacagattt ttccatatgg tttgatgctg ctccgccaac ggaaattaag
    14221 atacggattt gcacaaatct ttcagacggt acagttctga caatgcttcg cgcggggtca
    14281 ggctgtcggg atcgaggttt tccagggctg ctgccaatat accttcaaaa tgtttttcct
    14341 ctgctttatc cacaaagcag tccacattcg gttcatctcc tttttcagac ggcatggtac
    14401 tgaaaatatc cagttgggga cggtttgcgg cggcttggtt ttccagtccg ttcaaatgct
    14461 tttgggcggc tttcaatgcg cgcacaggca ggccggcgag tttggcgacg gcgatgccgt
    14521 agctttttcc ggcgggaccg ggttggattt ggtgcaggaa cacgatgtcc cgtccctgtt
    14581 cgagcgcgga aaggtgcata ttgacggcgg cggcgtgggc ttcgggcagg taggtcagct
    14641 cgaaatagtg ggtggcaaac aggctgaagg atttgttttt ttgcagcagg tgttcggcga
    14701 ttgcgtgcgc gagggcgagg ccgtcgaaag tggaagtacc acgtccgact tcgtccatta
    14761 aaacaatgct ttgttcggtg gcgtgatgca ggatgtaggc ggtttcgctc atttcgacca
    14821 tgaaggtgga gcggttggag gcgaggtcgt ccgatgcgcc gatgcgggtg aagatttgat
    14881 cgacaggccc gattgtggcg gcatcggcag gcacgaaaca gccggtgtgt gccaataaaa
    14941 caatcagcgc gacttggcgc atataggtgg atttgccgcc catattgggg ccggtgagca
    15001 gcatgaggcg gtgtttgtgg tcgaggtcgg tgtggttggc tgtgaagtgg cgtacctgct
    15061 gttcgacaac gggatggcgg ccgttttcga tgtggacaac cggatagtcg gcaaactcgg
    15121 gacggacaaa gttccgctct tttgccaagg ctgaaaatgt ggacaacacg tccagcgcgg
    15181 cggcggcttt ggcggctttt tgaagctgcg gcaatgccgt ccgaaggttt ttcaatacgc
    15241 cgtcaaagag ttgtttttct aaggcgaggg cttggtcttg agcagtcagc actttgtctt
    15301 caaaggcttt cagttccggc gtgatgaagc gttcggcgtt tttgagggtt tgccggcgtt
    15361 ggtagtcggc aggtgcttgt tcggcttggg ttttggacaa ttcaatgtaa aagccgtgaa
    15421 cgcggttgaa ctcgactttg agtgtggaca aaccggtacg ttcgcgttcc ttggcttcca
    15481 aatccagcaa aaattcgtcg ccatggtttt gaatgcggcg caattcgtcc agttcgggat
    15541 gaaaaccgtg gttgatgaca ttgccgtctt tcagccagac ggaaggttcg ggcatcacgg
    15601 cggctttgag ggtttcggca actggcaggg tttcggggaa aacggcttta agggtttcca
    15661 acaggctgct gccggttgcg gacaagtcga tttgtgccaa ctcgaacaga ctgtcgcgta
    15721 aagatgccaa atcgcgcggg cgggcattgc cgacggcgat gcgggcggcg atacgttcga
    15781 tgtcggcaat gctcttcaga tggcattgga ggggttcgta ttggctttcc agcgcggtta
    15841 cggcttcttg gcgcgctcgg atgtgggcgc ggttgcgtaa agggtggtgc agccacaatg
    15901 ccaacaggcg gctgcccatg tgggtggcgc agccgtcgag tatggaaaac agggtcggcg
    15961 tttttttgcc ggagagggtt tgcgtgattt cgagattgcg gcgcgtggcg gcatccatgc
    16021 cgatatattg gctgtcggtt tcgagcgaca ggccgtccaa atgttgcggc atcaggtttt
    16081 gcgtcaggcg gatatagttc aacagcgcac ctgccgcgcc aatcgaaacg gcgtgttctt
    16141 tgctgtccaa accgaagccg cgcaaatctt ggcagccgaa atattccgtc agcagttttt
    16201 cccccgcgtc ggcggcaaac tgccacgcgt tcaggcgtgt aacacccgat gccgtctgaa
    16261 gttgcggtgc gtttttactg tccggcagca aaatttccgc cgcctgcagg cgcgccagtt
    16321 cgtcgttcaa tttatccgca gttgtcagct tggttttgaa ttcgccgctt tgcagcgatg
    16381 cccacgccaa accgatgtat tttttgtcgg gagacacggc aacgatgcgg ttggtttcct
    16441 tgtcttccag taatgcggaa tcggttagcg tgccgggcgt tacgatgcgc acgactttgc
    16501 gctccaccgg ccctttgccc gcgccgactt cgccgacctg ttcgcaaatc gccacgcttt
    16561 tgcccaactt gaccaggcgc gccagatact gttccgccgc gtgaaacgga acgcccgcca
    16621 ttttaatcgg cacgccgtcc atctgcccgc gtgtggtcag ggtgatgtcc aacaatttcg
    16681 ccgcttccac cgcgtcatcc aaaaacagct cgtaaaaatc acccatacgg taaaacacta
    16741 gtttgtcggt atgttgcgct tttatgccga ggtattgctg catcattggg gaaacggcgg
    16801 atttggacat ggggctgcct tattattagg aaaacggtat tgtagcaaaa ggttttgtct
    16861 tcgacggaat gagactgacc gtaagcttgt caataaagtt gtggagacaa ataggaaaac
    16921 ggcaaagtta tccataagaa tattattaag tcaaaaaaca tccacgctta tctgtcttat
    16981 acggcaagcc ttaaactcaa atttatttaa caaaaaggta atgaaaatat tggatatttg
    17041 ctacttatac acaagcaggt cggcaatcat caaaatcatc attaaaattt taaaatcaaa
    17101 tattgaatgc gcgtttttga aatagcgaaa ttcacctgcg tcaaacaaaa cgcaagtaat
    17161 tttatgtaat agtggaatgt agttttattt cagaatacaa tacaggcatc ggactttatt
    17221 tcacaaccgg gagacaaaat atgagcatca aagtagcgat taacggtttc ggccgcatcg
    17281 gccgcctcgc attgcgtcaa attgaaaaag cccacggcat cgaagtcgcc gccgtcaacg
    17341 acctgacccc tgccgaaatg ctgctgcacc ttttcaaata cgacagcaca caaggccgtt
    17401 tccaaggtac tgccgaatta aaagacgatg ccatcgtggt aaacggcaga gaaatcaaag
    17461 ttttcgccaa tccgaatccc gaagaattgc cttggggcga gttgggtgtg gatgtcgtcc
    17521 tcgaatgtac cggtttcttt accaacaaaa ccaaagccga agcacatatc cgtgccggcg
    17581 cgcgcaaagt cgtgatttcc gcacccggcg gcaatgatgt gaaaaccgtc gtatatggcg
    17641 taaaccaaga catcttggac ggtagcgaaa ctgtcatttc cgccgcttcc tgtaccacca
    17701 actgccttgc cccgatggcg gcagtcctgc aaaaagagtt cggcgtagtc gaaggcctga
    17761 tgaccaccat ccacgcctac accggcgacc aaaacaccct tgacgcgccg caccgcaaag
    17821 gcgatttgcg ccgcgcccgc gccgccgcgc tcaacatcgt gcccaacagc accggcgccg
    17881 ccaaagccat cggtctggtt atccccgaat tgaacggcaa actcgacggc tccgcccaac
    17941 gcgtccctgt tgccaccggc tcgctgaccg aattggtttc cgtcctcgaa cgccctgcaa
    18001 ccaaagaaga aatcaacgcc gcgatgaaag ccgcctccag cgaatcttac ggctacaacg
    18061 aagatcaaat cgtttcttcc gacgttgtcg gcatcgaata cggctcgctc tttgacgcaa
    18121 cccaaacccg cgtgatgacc gtgggcggca aacaactggt gaaaaccgtt gcctggtacg
    18181 acaacgaaat gtcctacacc tgccaactcg tccgtacttt ggaatacttc gcaggcaaaa
    18241 tctaaggctt gaacaaacct gtggataagt aaatgccgtc tgaacacatt gcgttcagac
    18301 ggcattgttt atctgtaact tgatattgcg gcttatggcg aacaaacaac agccgtttgt
    18361 tcaactgcta tgcggcaaaa ccggattttg cccgatttaa agcggggttg gggaaagatt
    18421 aataaccgtc gcgcggtttt cctcgatttg aaacctgact tcgtaatccg caatattcat
    18481 cacataaatc cgttcgggaa tattctgata ggcggggcgc ggatcttggg caatgctttg
    18541 gctgataagg tttttggtgt ttgcagataa attttccgcg ccgatgtttt cctgccaaac
    18601 gacttccaac tctacgggtt tgccgctgac gaaaccggat gcggcatcgg gtttggattc
    18661 gataaagggg atataaggct tgatgtccac aatcggcgtg ccgtccagca ggtctgagcc
    18721 gctgcaatag aggcggacgg gtttgccggt ttcgatgcgt tcgagtttaa ggagagagag
    18781 tccgagatgg ttggggcggt gggggctgcg cgtggcgaac acgcccattt tttgtttgcc
    18841 gccgagccgt ggcgggcgca ccatttgcgc ccaaccttca tccaatacgc cgtggaagat
    18901 aaaacttatc cacacataat cgaaatcttc caacccgcgc acgctgtctg cggtaaattc
    18961 gggattcagc tcgatgcagg cttctgcggc ggagaccaaa ccgggctggc gggcgatgcc
    19021 gaatttctgt ttgtagggcg agcgggcggt gccgatgggg gtaatggtat aagtcataag
    19081 attgtggata agttttttgg aaaaacctta tcttatcaca gccggtttta ccgctttcgg
    19141 gtagtaattt gttataatac agggcattta aatcgcgttg aaataaagta gcacagaatc
    19201 ctcgtgtcca agacgctctt aaaaaggcat tggagtattg ataaaaaaga agcataacgg
    19261 ttcgttataa cgaagaggga atcccgtata ttacacccaa gtcaggttgt tatctttcct
    19321 tttggatctc acttacagga ggtgagatct gaaattttgg gtaataaaat cggattacag
    19381 cttctagcaa ggagtacctt aggtatagct aaaatggagg gggtggatat gacatatcat
    19441 atccacctag atggattgta ctccttaaat aaagaaggta agagttttgt tattcgtaaa
    19501 atagagaatg acgatcttaa atgaagaagt aatgagatga ttgtttccat tgatgttgat
    19561 gcacaaaaaa cttttacgcc tttgtgtcct gacgaactgc ccgtgaacga ggggcatttg
    19621 attgtcgagg agttaaatgc ccaagccgct ttggcggatt tgcgtgtgat gacgaaagat
    19681 gcgcatcata tggcggcgaa atggcttgtg ggtaaccctg ttgatatgtt gaagccgaca
    19741 ggtttgtccg atgcggattt gacttgggtg gctcatgcga tggtcggtac gcgcggctat
    19801 gaattgttgg acggtctgcc ttccgtcaag gaatacgatt attgcgtttg gaaaggtgtc
    19861 gatcccgaat tgcatcctta cggcgcgtgt tttcacgata ttgaggaaaa actgagcact
    19921 ggattgattg agtggctgcg ttgtcaaaat acgaatatgg tcattgtcgg cggcttggct
    19981 acggattatt gtgttaaaac aacggttttg cagttactta aaggcggtcg ttggcaggtt
    20041 atcgtgaatg aagcggcttg tcggggtatt gcgcccgata ccattgaagc agcgtggcag
    20101 gaaatgcgtt cttcgggtgc gataattttg aaaaacgccg aaaaaattaa aaaatatatt
    20161 aataatcaat aacttataat tgtttcacgt gaaacaccct gtctacatat gaaaatttta
    20221 cttgtccgtt tgtccagtat gggtgattta attcacactt tgcccgcaat cgaagatttg
    20281 gcgcgacaat gctctgatgt agaactgcat tggctatgtg aagccgggtt tgcgggtatt
    20341 gcacggttgc atccgtttgt aaaaaaggtc catgtaatga aatggcggca atggcgcaaa
    20401 catctctttc aggctgaaac ttggcgggag atgggtcgtt tgaaacaggc tttgctgcag
    20461 gaagcatttg attttgtatt ggacagtcag ggtctgatta aaagcgcgtg tttcgccaaa
    20521 atggcaaaat cccctattta tggtttggat aaacacagtg cgcgtgaggg ggtggctgct
    20581 ttggcgtatg ataaaaagta tgtcgtaccg aaaggtagaa atgccgtttg gcgcaaccgt
    20641 gatctgtttg cccaggtgtt tgggtatgca atgcctgaga cgcaggtatt tggtttgtcc
    20701 gttcctgaag caagccgtct gaaaaattta gcgcagccgt attatgcggc tttgcacgcg
    20761 accagtcggg acagtaagtt atggcctatg gaaaattggc gggagttgct gcagaaactg
    20821 aatcaaaaac aacaatgcaa tgtttatctg ccttggggaa acgaggctga aaaagtgcgt
    20881 gcggaacaga ttgcagacgg gctgccgttt accatagtgt gcgctaaaat gaatttatta
    20941 caggcggcgt atctgttgaa acacgcggtt ggaatagttg gtgtagatac cggtttgctg
    21001 catttggcaa atgccttgga aaaacctgtg gtcggcattt ataccgatac cgatccgatt
    21061 aaaacaggcg ttcaagtttc ggctattgca aaaaatgtgg gcaatatcgg gcagattccg
    21121 accgcagatt tggtttatca aacgctgatg gattgtgttt cagcagataa aggctaaagg
    21181 gtcgtctaaa actgatatgg cggcatgata ttttccaatt tattagatac aggaatgtat
    21241 atccgccaga ctgagtaaat actttaaaaa tggtataatc ggtaaaatta tccgaaagat
    21301 tgctaaataa attcgttttt aaggaaaacg gatttgattt tataaagaag aaggtttggt
    21361 ttcgctggat ggtgaaagca gcgttctttt taggagaatt caatgaaagc actggtcgca
    21421 gtaaagcgcg tagtggacta caacgtcaaa gtccgtgtga aagccgatgg ttcggatgtg
    21481 gacatcggca atgtcaaaat gtcgatgaac ccgtttgatg aaatcgctgt ggaagaagcc
    21541 gtccgtttga aagaagccgg gaaagtaagc gaaattgtag cggtttcttt gggtgaaaag
    21601 aaatgcgaag aaaccttgcg taccgctttg gcaatgggtg ccgaccgtgc cattcatatt
    21661 gaaaccgatg tgaaattaga gccgctggca gttgccaaac tgctgaaagc tgttgcggac
    21721 aaagaaaatc cgcaaatttt ctttttgggc aaacaagcga ttgatgacga tgccaatcaa
    21781 gtggcgcaaa tgctggcagc tttgttgaat gcggcgcaag gtacatttgt ttcaaaagta
    21841 caaattgaag gtgatgaagt acagattgtg cgcgaaattg atggcggcga agaaacgatt
    21901 gcgttgaaac tgcctgcagt catcagcgca gatttgcgtt tgaacgagcc gcgctttgtc
    21961 aaactcccca atattatggc ggcaaagaaa aaacctctgg aaaaactgga ttctgccgat
    22021 ttggctaccg atatttcgcc tcgtctgaaa acggtgaaat ttgccgaacc taaggcgcgt
    22081 cagacaggtg taaaagtagc aagcgttgcc gaattggttg aaaaattgaa aaacgaagcc
    22141 aaagtgattt gaggaggctg aaatgagcgt attgattatt gccgaacacg acaacaaaca
    22201 gttgaatcct gccactttgc atgctgttgc cgctgccgcc aaactgggca aggtcgattt
    22261 attggttgtc ggaaacggcg catcgtccgt cgtggaattc gcgaagcaag tagcgggagt
    22321 ggaaaaagtt ttggttgcag atgctgctca ttatgctgaa ggtttggctg aagagttggc
    22381 tccgctggtt gtcaaattgg cggcggatta ccgctatgtt gcagcaacgg caaccacatt
    22441 cggtaaaaac cttttgcccc gcgtagccgc cttattagac gtaccgcaaa tttctgattt
    22501 gaccgagatt gtagataaca cgacttttgt acgtccgatt tatgcaggta atgcatttga
    22561 aaccgtgcaa gccgattcag aaaaattggt gctgaccttc cgtgtgacgg cttttgacgc
    22621 agtggcggcg caaggtggta atgctgaggt aattaatgtt aaaacaaccc ctgcccaaaa
    22681 cctgagtcgt tttgttaacc gtcaactttc ccattccgat cgtcccgaac tgactcaggc
    22741 aaaagtgatt gtttccggtg gtcgtgcgtt gggtagtgtg gaaaaattca gtgaagtgct
    22801 gacaccgttg gcggatgttt taggtgcggc aatcggtgca tcccgtgcgg cggttgatgc
    22861 cgagtatgca ccaaacgatg ctcaagtcgg acaaaccggt aaggtggttg cgccgcagct
    22921 ctattttgca atcggtattt caggtgcgat tcaacacgtt gccggtatgc aggacagtaa
    22981 ggtgattgtt gcaatcaaca aagatgccga tgcgccgatt ttcaatgtag ccgattacgg
    23041 attggttggc gatttatttg aaattgttcc acaattaata gaattattga aaaattgatg
    23101 tttcacgtga aactttcagg cgactttgtt attaatgagg tcgtctgaaa acatttcagc
    23161 atgattacga cttataaaac cattacttcc ccgacgcagg ctgagtttaa agataaaggc
    23221 agccgtttta ttgcatttgc ctatccgatt cggacattgg ctgatgtgaa aaaatacctc
    23281 aatctgttaa aggaagagca ttataaagcg cggcattggt gctatgccta tcgtttaggt
    23341 gtgaacggga tgcagtttcg cggcaacgat gatggagagc caccaggaag tgctgggcgg
    23401 ccaattttgg gacagattga ttcggtgggt atcactgatg ttttggtcgt ggtcgtccgc
    23461 tatttcggtg gtgctttatt gggcgttccc ggcttgattc acgcatacaa agaggcaacg
    23521 gctcaggcgt tggcgatcgc ggaagtggtt gaaaagaata ttgaaaaaac cgtttggctg
    23581 aaatgtgagt atccgttttt gaatgaagca atatgcattg ccaaacaata tcaggcggat
    23641 atattggagc aggatttgca gttggattgc cgattgacgg taagtttgtc gttggtaaat
    23701 tatgaggcct gtgttgcggc ttggaagaat acacggcagg ttgaagtaaa tacggaaaag
    23761 ccttttgaat gaaaggttgt atgaaatgct ttgtaatggt atttcaggca acctcttcgc
    23821 tgattaattt tcttgtgtat atctgtctaa ggtatcgacg ctggagcata aatatagcca
    23881 agccaattga tggaattcgc ccggcgcgtt ccataatgca tcttcactca taatgctata
    23941 ttcgccttct gtttgcaaat tcagttctac cccgtcttca atagtctgat ggcgcacctg
    24001 atactcgtgg gtatagaaat tgtccatatc gcgaattaaa gcgacggcat atttccaacg
    24061 gcggatgttt tcttccagtt gcagtagtaa atgacaccat tcgcggtatt tgctttggat
    24121 ttcatcaata cgtttttgcg tgtttgcttg ttctttttta atttgtgaat aagttgaata
    24181 ggttgcagag tggaatgaag tggtcttttg tttgattcta ctaaaatttc ttcagatggg
    24241 acaaaagttc ctttgtatcg agttttcgtt aaaagtacat atttgtcaag gagatggcat
    24301 gaaactgctg gttatcggta atggcggtcg cgaacacgcg ctggcttgga agttggcgca
    24361 gtcgcccaaa gtggaaacag tatttgttgc gcccggtaat gccggtacgg cgattgaatc
    24421 caagctccaa aacattgcct tgaccgcgta tcaggatttg attgaattct gccgtaaaga
    24481 aaatattgtt tttaccgtcg tcggccctga agcgcctttg gcggcaggta ttgtggatga
    24541 ttttcgtgcc gcagggctga aaatatttgg tccgacacaa tatgcggcgc agttggaaag
    24601 ttctaaagat ttcgccaaag catttatggt gaaatacaat attccgaccg cgcaatacca
    24661 aacctttgaa aatgccgatg ccgcacatga ttacgtcaat cagaaaggtg cgcctatcgt
    24721 tatcaaagcc gatggtttgg tggcaggtaa aggcgtgatt gtggcaatga ctttggatga
    24781 agcgcatgct gcgattgacg atatgttgtt gggcaataaa atgggcaatg ccggcgaacg
    24841 cgttgtgatt gaagattttc tgcaaggcga agaagcgagc tttatcgtta tggttgatgg
    24901 caatcatgta ttacctatgg cgaccagtcg agaccacaaa cgtcttttag acggagataa
    24961 aggcccgaat acgggcggta tgggtgcgta cagtcccgcg cctgtggtaa cgcctgctgt
    25021 gtacgagcgt gcgatgaacg agattatttt gccgaccgta gcgggtatga aagcggaggg
    25081 gcacgagttc accggctttt tgtacgcagg tttgatgatc gatcaaagcg gtgcacccta
    25141 tacgattgag tttaactgcc gtttcggcga tcccgaaacc cagccgatta tgagccgtct
    25201 aaacagcgat ttggcggatt tggttgaagc ggcaatagac ggcagactgg acagcgtcaa
    25261 agcggaatgg aatccgcaaa ctgccgtggg tgtggtactg gcggcgcaaa attaccccga
    25321 aacacccaag aaaggcgatg ttatttccgg tttggatgac gtcaaccgaa tcggcaaagt
    25381 attccatgcc ggcacaacgg taaacgagaa aggcgacgtg ttgaccaacg gcgggcgcat
    25441 attgtgtgtt gtaggattgg gcgacgatgt ggcgcaagcg aaagccaagg cgtatggcgc
    25501 gttggaaaaa atcagcttcg acgggatgca ataccgtaaa gacattgccg ataaagcgat
    25561 taaccgttaa ttagaaagag gatgatgccg tctgaagcca agtatgactg atgcgcttca
    25621 gacggcattt tatattatgt tattttcgag aattatcgca gcatcagcgc aacgtaagtt
    25681 aagcgtttat ttgaaaaaag gcggtttggt tgcttatccg accgaatctt gttatggctt
    25741 ggggtgtctt ccgacattag ccaaagcact cggtaagttg gcgcatttga aaaaaaggcc
    25801 gcaacacaag ggtatgattg tcatcgggaa tcaacttgaa caattgcagc ctttacttca
    25861 aatgccgtct gaaaacattc aaaccatgct tagaaacgaa tggcctgctc ctaagacttt
    25921 cctattgttg gctaaatctg gcgtattacc tgcgttgcgc gggaagaggc gcagcaagct
    25981 tgcggtcaga gtgcccgatc atacaggtgc aaggcgtttg tgtcaggcat tgggtatgcc
    26041 tttggtttcc acttcatgta atcgggcggg taaacgcgcg tgtcggacgg agagggaagt
    26101 cagaaggcag ttcggacgcg atgtgtggat tgtcggtggg agaatcggca ggcagaaatc
    26161 gccgagtcag attatcgatg gggaaacagg taaacgtttg cgttagatat ggatgcttga
    26221 gtgaaaactt ttttgcatac acaaagaaaa aattttttgg atggtatttc tctacatgat
    26281 tgttatcgca ctgcgataaa ggtagaagga cgcaacgtct gctttgattt tgaggacggt
    26341 tttgtcgttc tggacaataa tccgaacaat caagcgggca agcatctgaa aacagatttt
    26401 tcccgtgttg ttttaaccca tgaaaataga aacgccgaag acgactattt ggtcgatatt
    26461 tttgaagaca tcgtcttttt agggaaacgt ttgtttacag tcagaaaatt tcttgatttt
    26521 tctgaattgc ttgaaatgat caatgcacaa ggctattttc ttgaatttct gtatctgtat
    26581 gaatgtatcg gtgtcaaaac aagcgattat tttttagagg ctgtgttggt tacgggacgg
    26641 agccgggaat gtaaaaaatg ctttatcaaa attatgaggg caagcagttt tgtgtatcaa
    26701 tggaacaatt tacgtttgaa tagtgagatt gtatagcggg tttatatttc ggatggaagc
    26761 cggtttaaaa aaataatgtg gataatttca gggaatggca aaatgtcgtc tgaaatgctg
    26821 atatttcagt attccagacg gcatttttat aataaggctt atggataaat ttgtgtataa
    26881 gttgaaatca gagttttttc ccgcatacgg tttcatattg tttttcagtt aatgtcccgg
    26941 acaggatgct catcgggcgg agggaagagg ctgtgtagtt ttgtgtggaa atttccgtgt
    27001 tttttgtatc aaataactgt agcgaactta agcggtatgt tttgttgttg cagtggattt
    27061 cccactcggc aatggcagtc ttgtatgcgg gggtgttggc aaaacgttct tgtttcagat
    27121 tggtaacaac ttttttatct tggaaaatca tcagatttcc gttttttctc acactgtctt
    27181 tattaatata tgtatgaata ttgccgtttg agattgtgcc gatattttcc catcgggcag
    27241 acttgtccgg tgtgctgcag gcggttatca aaatagggag taaaacgaaa gataggaaga
    27301 ttgggcgcat tagggtttcc ttaatcattg aaatccgctc attcagaaag aacgggttgg
    27361 gccggagggg taatgccgtc tgtgaaatga ggcaaatatg ggaaattgat gcagccttcg
    27421 tgttttacgt atttatatat tccgtatgga ggaatatgct gcttttgaag agtgtattat
    27481 ggatgaactg ccgataacag taaatcggtt tgatttgttt ccgtcagctt atgccgtctg
    27541 aagccctttc agacggcatt tattttgaag ttctggttgt ttttgccagt gatattttga
    27601 tggtttgaag ctgtctttta tattcccggc aatgcgggca gaacagcagg tgcatatata
    27661 tggaaatctt ctcgcccggg gtggtttccc ggtcctgatg tttggaaaga agcagggcaa
    27721 tatcgcggca ttttttcata acgtttactt cgggttttct tggttgaacc atttgatttg
    27781 caggcattgg cgcaatgatt ctcgggcgcg gtgcataatg gtgtggtagt tggacgtgct
    27841 gataccgcac atttgttgta tttcgttgga tgaaaaaccg agtatttcct tcagggtgaa
    27901 tacccgtgcg gtgttttcag gcaggttgta taggcagctt tgcagaattt tttggaattc
    27961 gttgttattt aatgattttt ccggagtgtt ccaatgttgc ggctgccctt ccggcgtcca
    28021 atgcccgttt tgggaaaaat ggctttcaaa tgcttcatcc agcagctcgt catccagtgc
    28081 ggtaaagact ttcctctgcc tcccgatttg acgtaatgcg tcaataattt tgtttttcaa
    28141 tatggcaaaa agccagctgt tgacaagtgc cctgccttga aaactgtcgc ctgcgctgta
    28201 tgcggacagc aatgtttcct gcactaaatc ttcagccaaa tcagggtggt cgggcaactg
    28261 gagtcgcgca aaatgcagaa gcagtttgcg cgactccatt aattcggcat cggtcaggtc
    28321 gggtagcggc atagtatcgg gaacaaggta attttatgcg gatagatggt tttggctcgg
    28381 ggaattgcct tccatatttt cggatatggc ggacaaggag gggatgataa tcctttgttc
    28441 aatccagcct gaccatttcc caagcaaaat atttttccaa ttatcggctt taggcataaa
    28501 ttcgacaagg gtttgggcga gggtgtcaaa cgaaagcgcg gaggaaccca ttatctccag
    28561 cagcatcata tcgaagccgt ccaatgtttg gtacatcaca tcatcttcgg cgtttcgcca
    28621 tattaacaag gctgtttccg cttcctgcaa atcatgggta acatcgtatc gatattgccg
    28681 gataaaggcc gcaggggagg gcgtgtattt gctgtcattt gaataatgaa tgtcgggaat
    28741 atccggaatt tgtgcaactt ctgccagcaa ttgggtatat tcaaaatcca tcagtgccaa
    28801 aatgccgtct gaaagcggca ggctttggca atattgtagg aactcgccgg ggatttcttg
    28861 aaaatagggc gtttgggcac gcgcgtcgcg gacgaaacct tctttcagac ggctccattc
    28921 tttgctgtca aagtattgcc gcgtttcggt atagcagcgg tcgataaagc tgtggatatt
    28981 gttgcgtatc aggcggatat agacgttcaa tcggtcttgc ggcagaccgt ctgcggcttc
    29041 gcccccgcgt atggcttggg agaaacggtg ctggtattgg gcggaggttt caggctgcac
    29101 ggcggtattc ctttccggca cgcgtttgat aatcggcaat tttggcaact tcggcttcga
    29161 gttcggcaaa aggtgggaaa ttgaaatcgc gttccaacag ggtgggaggg atggtgggaa
    29221 gcttggtgta ggcgagttcg agcaagtccc aaacggtcgg taaaaccgcc gcgccgtggg
    29281 tatcaatcaa aagctcgggg gtttcggcat cgtgtccggc gatgtggatg tagcacacgc
    29341 gccctgcgtc cacattctct aaaaaggcct caggcgacag caggccgtga ttgacggcgt
    29401 tgacgtaaat attgttcaca tccaaatgaa taccgcagtc ggcttcacgg gcaaccgcgt
    29461 tgaggaactc gacctcgttc atctcggcaa gcggagaatg caggtagtag gacgtgtttt
    29521 ccacggcgat gcggcagccc aaacggtctt gcacttcgcg gatacgccgc gccgtatgat
    29581 gcaccatttc ttcagtgaaa ggcagcggca acaaatcgta aagatgaccg ccgtcgtggc
    29641 agtagctcaa atgatcggag aaaaacgtgc agtcataacg gcacatcatt tctttgatgc
    29701 cgtctatcaa atcggtatcc agcggggctt gtccgcccag cgacatagat aatccgtgca
    29761 acgccagcgg cagccgttcc gccacacggt caaactgttt gcgcgccctg ccgcccattt
    29821 tcagccagtt ttccggtgcg gcttcgataa agcatatcgg gctgttttcg gaaagcgaga
    29881 gaaagtcttc cgccaagtcg cggcggtagc ccaagcctgc gtgttgaatc atatttttac
    29941 ccggttcgga tggcacaaaa gaataatgtg cttgtttaaa aaaatctttg tttgccgtta
    30001 aaaaagccgc attttactga aaaacgcggc ttggtttgaa ggggagggat tatttagaac
    30061 cgcatttgcc ttcgccgcat ttgccttcgg cagatttggc tttggcttta gatgctttgg
    30121 tgtgtttgtg ggcttttttt acagttgcac cgcatttgcc ctcgccgcat ttgccttcgc
    30181 ctgctttaga agcagccgcg ccgcacgaac cttcggcaga tttggatgcg ccgcaagagc
    30241 cttgggcgga tttttgaacg cctgttgcgt tgcttgccgg tttgtgggcg gcaacggcgc
    30301 cggcggccaa agacagggat aaagcaccgg cgagtgcggc agcaatattt ttgttcattt
    30361 ctatttcctt ttcagaatat caagttaacg agataaggtt ggaatgttta aagtcggatt
    30421 ccacgactga atcaatcttg tttcagacgg catttggggc aaaaccgttt tttcagcagc
    30481 gtatccagcg accatccgcc cgcaccctgg aaaagcagcg ggattaatac cacgatataa
    30541 attaaagcca ttttataacc gttgtcgcag acattgtaac ccgaaccggc gtgaaccgca
    30601 gcccaagcga cggcggtaac gaccatcagc cccaatgccg acaggcgcgt tgccaaaccc
    30661 aaaagcaaca gtacgggcag caaaagctcc gcatacatgg cgaaattcca gtttaacgcg
    30721 tccggcagca agttgaacgg gaatggaaac tgatcgttga tttcggaaaa ccaattctcc
    30781 ccgttccatt tttgcaaacc cgattccaaa aattcatatg cggcaaacag acgcaaaacc
    30841 agcatagcgg atgccgtctg ccaagttgtt ccgattttca taagtgttcc tgtttttgtt
    30901 cctgttgata ccccgttaga cggattttgt cggaaaaact gacacccgtc agctaataat
    30961 ttaacaagtt aatgaatata aaaagaaagt attttgacgg aaagaaaggg aaaaaggcgg
    31021 gatatttgga taacggcggt gttgtgagat aattcccgcc ttggacggta tgccttcaaa
    31081 tacaatttcc gtaaaggaaa aaaagaaaat attaattttg atgatgactt aacaaagccc
    31141 ctttcgcttg gctgtcaaag gagattgtta agaaaagcaa tctccctttg acggggtgcg
    31201 atatgtaaag gaatggaaaa tatggtaacg ttttcaaaaa tcagatcatt tttggcaatc
    31261 gccgccgccg cgttgcttgc cgcctgcggt acggcgggaa acaatgccgc ccgcaagccg
    31321 gtgcaaaccg ccaaacccgc cgcagtggtc gctttggcac tcggtggcgg cgcatctaaa
    31381 ggatttgccc atataggaat tgttaaggtt ttgaaagaaa atggtattcc tgtgaaggtg
    31441 gttaccggca catcggcagg ttcgatagtc ggcagccttt tggcatcggg tatgtcgccc
    31501 gaccgcctcg aattggaagc cgagatttta ggtaaaaccg atttagtcga tttaaccttg
    31561 tccaccagtg gttttatcaa aggcgaaaag ctgcaaaatt acatcaaccg aaaagtcggc
    31621 ggcaggcaga ttcagcagtt tcccatcaaa tttgccgccg ttgccactga ttttgaaacc
    31681 ggcaaggccg tcgctttcaa tcaagggaat gccgggcagg cggttcgtgc ttccgccgcc
    31741 attcccaatg tgttccagcc agtcatcatc ggcaggcaca aatatgttga cggcggtctg
    31801 tcgcagcccg tgcccgtcag tgccgctcgg cggcaggggg cgaatttcgt gattgccgtc
    31861 gatatttccg cacgtccgag caaaaatgtc ggtcaaggtt tcttctctta tctcgatcag
    31921 acgctgaacg tgatgagcgt ttccgtgttg caaaacgagt tggggcaggc ggatgtggtt
    31981 atcaaaccgc aggttttgga tttgggtgca gtcggcggat tcgatcagaa aaagcgcgcc
    32041 atccggttgg gcgaggaggc agcacgtgcc gcattgcctg aaatcaaacg caaactggcg
    32101 gcataccgtt attgacgcta tgccgtctga acgtatttcc agaaggcttt atccatagaa
    32161 aaaaataccc gaatcccgta caatacgccc ctaaaatttt gaaagaacga aagaatcatg
    32221 gaagccgaag taatcaacca gctcaacaac accctgaatg atttggagaa gcgtagcgaa
    32281 gacatccgcg tttatatgga ttatcagggc aagaaagacc gattggaaga agtcatcggg
    32341 ctttccgaag accccgagct ttggaacgac ccgaaacgtg cccaagaaat cggcaaagag
    32401 agcaaaatcc ttgaaggcat cgtgctgacg ctcgacaaca tcgcttcggg tatcgaagac
    32461 aaccgtatgc tgattgaaat ggccgtcgaa gaaaacgacg aagaaggttt tgccgccgtg
    32521 aaggaagacg tggcggggct ggaaaaacag atggcggatt tggagttcaa acggatgttc
    32581 aaccagcccg ccgacccgaa caactgcttt atcgacatca ccgccggcgc gggcggtacc
    32641 gaagcggaag actgggcggg gatgctgttc cgtatgtaca gccgctatgc cgagcgcaaa
    32701 ggcttcaaaa tcgaaatcct tgaggaagac gacggcgaaa tcgcaggcat caaccgcgcc
    32761 accatccgag tggaaggcga atacgcttat ggtttgctgc gtaccgaaac cggcgttcac
    32821 cgtctggtgc gctactcgcc gtttgattcg aacaacaaac gccatacctc gtttgcctcc
    32881 gtgttcgttt accctgaaat cgacgattcc atcgaaatcg aaatcaaccc cgccgatttg
    32941 cgcatcgaca cctatcgcgc atcgggtgcg ggcggtcagc acatcaacaa aaccgactcc
    33001 gccgtgcgta ttacccacga gccgacgggg attgtggtgc aatgtcaaaa cgaccgttct
    33061 caacacgcca acaaagccgc cgcgatggaa atgttgaaat ccaaactgta tgaattggaa
    33121 atgcgcaaac gcaacgaaga gaaacaggca ttggaagaag gtaaatccga tgtgggttgg
    33181 ggcagccaaa tccgttcgta tgttttggat tcctcacgta tcaaagactt gcgtacaggc
    33241 tacgaagtcg gcaacaccaa agccgtattg gacggcgact tggacggctt tatcgaagcc
    33301 agcctgaaac agggtgtgta gtttaggctg tatgccgtct gaaacctgta ccgtttcaga
    33361 cggcattttt gcgtttcgtg ttataatccg cgccgcacac gggacgggca gacagtcgcc
    33421 gcgtatcgcg taaggcatac ggggaggaaa gtccgggctc cgcagggtag aatgccggtt
    33481 aacggccggg cgcggtaacg cgacggaaag tggaacagaa agcaaaaccg ccgatggctg
    33541 ctttggcagc acaggcaagg gtgaaaaggt gcggtaaggg cgcaccgcgc atttggtaac
    33601 aatatgcggc aggccaaacc ccattcggag caagaccaaa cagaacgcaa tgacgctgcc
    33661 cgccgagcgt tcgggtaggt tgcttgagca taccggcaac ggtatgccta gaggaatgac
    33721 tgtccgagac agaacccggc ttaccgcctg tccggtgtgc atccgacacg caaatgccgt
    33781 ctgaaacctg aaatacggtt cggacggcat ttttatttag gctgtgtttt tatattccgt
    33841 tgatgcagcc ctgatgagtc ggacaaaatg ccttttactc aataggaccg gtttccgccc
    33901 aagcggaaat aaacggctat ttcccttgca aacagacgga atcaggcggg tatttaaatg
    33961 acaatcggca ggctgccttc cggttggggg ggaaagcctg tcctgtcggg tggaaacggt
    34021 ttggtttcat tatgcggaaa aatgccgtct gaagggttca gacggtatcg gcgcatcggg
    34081 gcaggactta gtctgtgcct tcgagcattt tgttcaattt ggggacgagc aggagcagca
    34141 ggaagcctgt ggctgcgccg atgtagaaca gcagccggta gaagccgatt tcgtcgcccg
    34201 cctgatagcc tttttcaaac aatacgccgc ccaaagtgaa gcctaatgaa aaggcaagga
    34261 aattaagggc gaccatttgg gttttgaata aaggcggtgc gattttggtg gagatggaca
    34321 gcgcaatcgg ggaaatcatc agttcgccta tcgtgatggc gaggacgatc agtgcgaaaa
    34381 ccgcaatagg catcggcgtt ccggaggaaa taaagggaac gaatcccaaa aacgacgcgc
    34441 cggtaacaaa taccgccata gcgaatttca gcggggtttt gggttgtttg cgccccattt
    34501 ttgtccacat tgccgccatg agtccggaaa acaggatgac ccacaggctt tgcatagaat
    34561 ctttccaggc gacgggtacg gtaaacgaac cgatggtgcg gttgacggtt tcgtcgaaat
    34621 agacggttgc cacggtgtaa atctgaaacc agacggccca aaacatacag atggtcagga
    34681 aaagcgggat gtaggcgatg atgtgccgtt tgttgtcgga actgacgcgg gggttggtca
    34741 gcaggcgggc gaaataggcg atgacggcaa ggatgacggt agataagagg atgcgggaga
    34801 aattgtcgag gttgacaagc ccggttttga tggcggttgc aagtgcggcg atgagggtga
    34861 tgccgacggc ggccgcagtt ttgccctgtc cttttgaaag cggatggggg acggtggggt
    34921 ggggcaggtt tttacgtccc aaggaataac gccacaagcc gaatgccata ccgaccgccg
    34981 ccgcgccgaa accataatgg aaaccgatgt tttcttgaag cagtccggtc aataaggggc
    35041 ctaagaagcc gccgatattg atggcgatgt agaaaatgga aaatcccgca tcgcgcagcg
    35101 ggcgcatttc gtcctgttcg tataatgcgc ccaccataga actggccgta gatttcacgc
    35161 cgccgctgcc caatgcgatg aatatcagcc cgattagaag gccgtacagg cccggtgcgg
    35221 cggcaaggac gatgtgcccg agcattacga cgatgcccga gaggaagagg gttttttccg
    35281 caccccatac gcggtcggca aaccacgcgc ccaaaatggt ggacaggtac acgctgccgc
    35341 tgtatgcgcc gacaatgccg cctgcgaggg ttttgtctat gcccaagccg cctttgtcgg
    35401 cggtgtagta gaggtaaatc agcaggatgc cctgcattcc gtaaaatgaa aaacgttccc
    35461 acaattcgat atggaagagg gtggaaagct ggaaggggtg gccgaaaaat gttttttctc
    35521 cggtgcgggc gggatgccgg gacataaagg ttctcctaaa agattttctc tcaataccgt
    35581 ctgaaacgga tgcggacggc ggcggtgttt ttttgtatga aaatatcggt ttttaaccga
    35641 tattttcatt ctttgtcaaa cgacgcgctg ccgtttttcg cgggcggatg tttttatatt
    35701 tgtttcaatt caatggattg tattttagag gacgtgttcc gatacggcgc gggtaaatcc
    35761 ttttctgtca atggcttatc cgatagggcg gtttttactt agatggaaaa tcacttcctt
    35821 atatatccgc cacgccgaag ggcgcattat ggtggattaa ctt
//
LOCUS       DAWTHD010000007        33947 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWTHD010000007 DAWTHD010000000
VERSION     DAWTHD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33947
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.007"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(20..1330)
                     /locus_tag="WM704_000257"
     CDS             complement(20..1330)
                     /locus_tag="WM704_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFA9339771.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(1466..2644)
                     /gene="ftsZ"
                     /locus_tag="WM704_000258"
     CDS             complement(1466..2644)
                     /gene="ftsZ"
                     /locus_tag="WM704_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFA9339772.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2763..4007)
                     /gene="ftsA"
                     /locus_tag="WM704_000259"
     CDS             complement(2763..4007)
                     /gene="ftsA"
                     /locus_tag="WM704_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFA9339773.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(4093..4821)
                     /locus_tag="WM704_000260"
     CDS             complement(4093..4821)
                     /locus_tag="WM704_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFA9339774.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(4811..5725)
                     /locus_tag="WM704_000261"
     CDS             complement(4811..5725)
                     /locus_tag="WM704_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFA9339775.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(5837..7246)
                     /locus_tag="WM704_000262"
     CDS             complement(5837..7246)
                     /locus_tag="WM704_000262"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFA9339776.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(7401..8468)
                     /gene="murG"
                     /locus_tag="WM704_000263"
     CDS             complement(7401..8468)
                     /gene="murG"
                     /locus_tag="WM704_000263"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFA9339777.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8472..9743)
                     /locus_tag="WM704_000264"
     CDS             complement(8472..9743)
                     /locus_tag="WM704_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFA9339778.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9922..11259)
                     /locus_tag="WM704_000265"
     CDS             complement(9922..11259)
                     /locus_tag="WM704_000265"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFA9339779.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11344..11724)
                     /locus_tag="WM704_000266"
     CDS             complement(11344..11724)
                     /locus_tag="WM704_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFA9339780.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(11849..12931)
                     /locus_tag="WM704_000267"
     CDS             complement(11849..12931)
                     /locus_tag="WM704_000267"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFA9339781.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13176..13352)
                     /locus_tag="WM704_000268"
     CDS             complement(13176..13352)
                     /locus_tag="WM704_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339782.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(13355..14713)
                     /locus_tag="WM704_000269"
     CDS             complement(13355..14713)
                     /locus_tag="WM704_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFA9339783.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14838..16484)
                     /locus_tag="WM704_000270"
     CDS             complement(14838..16484)
                     /locus_tag="WM704_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFA9339784.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(16507..17985)
                     /locus_tag="WM704_000271"
     CDS             complement(16507..17985)
                     /locus_tag="WM704_000271"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFA9339785.1"
                     /translation="MFSKLTPLAETNIPPLLCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVVNGAAFVFWDDDGKFAWNPEWKVPNQGIKNLKHRAGMLAAQVYDN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(18010..19761)
                     /locus_tag="WM704_000272"
     CDS             complement(18010..19761)
                     /locus_tag="WM704_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HFA9339786.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHSYYGGVVAGPPFKKIMGGSLNILG
                     VSPTKPLTNVAAVKTPS"
     gene            complement(19822..20085)
                     /gene="ftsL"
                     /locus_tag="WM704_000273"
     CDS             complement(19822..20085)
                     /gene="ftsL"
                     /locus_tag="WM704_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFA9339787.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(20112..21068)
                     /gene="rsmH"
                     /locus_tag="WM704_000274"
     CDS             complement(20112..21068)
                     /gene="rsmH"
                     /locus_tag="WM704_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFA9339788.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(21065..21520)
                     /gene="mraZ"
                     /locus_tag="WM704_000275"
     CDS             complement(21065..21520)
                     /gene="mraZ"
                     /locus_tag="WM704_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFA9339789.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            21799..23046
                     /locus_tag="WM704_000276"
     CDS             21799..23046
                     /locus_tag="WM704_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9339790.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            23142..23218
                     /locus_tag="WM704_000277"
     tRNA            23142..23218
                     /locus_tag="WM704_000277"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23176..23178,aa:Met,seq:cat)
     gene            23226..23301
                     /locus_tag="WM704_000278"
     tRNA            23226..23301
                     /locus_tag="WM704_000278"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23259..23261,aa:Ala,seq:ggc)
     gene            complement(23487..24308)
                     /locus_tag="WM704_000279"
     CDS             complement(23487..24308)
                     /locus_tag="WM704_000279"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFA9339791.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMFWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(24341..24985)
                     /locus_tag="WM704_000280"
     CDS             complement(24341..24985)
                     /locus_tag="WM704_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFA9339792.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDGTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(24988..25851)
                     /gene="ftsN"
                     /locus_tag="WM704_000281"
     CDS             complement(24988..25851)
                     /gene="ftsN"
                     /locus_tag="WM704_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFA9339793.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKARKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(25957..27453)
                     /locus_tag="WM704_000282"
     CDS             complement(25957..27453)
                     /locus_tag="WM704_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFA9339794.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27467..27787)
                     /locus_tag="WM704_000283"
     CDS             complement(27467..27787)
                     /locus_tag="WM704_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFA9339795.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28275..29801
                     /gene="putP"
                     /locus_tag="WM704_000284"
     CDS             28275..29801
                     /gene="putP"
                     /locus_tag="WM704_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFA9339796.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            30053..33658
                     /gene="putA"
                     /locus_tag="WM704_000285"
     CDS             30053..33658
                     /gene="putA"
                     /locus_tag="WM704_000285"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFA9339797.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVKAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSVNTTAAGGNAGLMAVAD"
     gene            33733..33836
                     /locus_tag="WM704_000286"
                     /pseudo
     CDS             33733..33836
                     /locus_tag="WM704_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         7976 a         9772 c         8420 g         7779 t
ORIGIN      
        1 atttttaata tcgaatcaat cagcccaaat accagaattt caatgcccac aatacggcaa
       61 caacccatac cataggcggc acgtccccag tccggcgaca caaaagtttg accacggcat
      121 agctgatgaa gccgaaggcg atgccgtctg caatcgaata ggtaaacggc atgaagacaa
      181 tggtcaggaa cgcgggcgcg gcttcagtca tatcgtccca atcaatgtcc ctcgcactgc
      241 ggagcatctg cgtgccgaca taaagcagtg cgggcgcggt ggcaaatacc ggaacacttt
      301 tcgccaatgg ggagaacatc agacacgcca gcatcaatac gccgacggta accgccgtca
      361 ggccggtccg tccgcctgcc gatacgcccg ccgcgctttc cacataaggc gtggttgaag
      421 aagtacccaa agccgcacct gccacaatgg cggtagagtc tgcaagcagt gcgcgtttca
      481 ggcggggcag cttaccgtcc accagcagtc cggcacggtg ggatacgccg accagcgttc
      541 cggtactgtc aaacaaatcg accaagaaga agacgaaaat cacgctgacc atgctgacgg
      601 taaacagacc tttaaaatcc atctgcataa aggtcggcgc aatgcccggt acttcgccga
      661 ccacgccgtg aaactcgttc aaacccatca ggctggcaat gacggtaatc gtcagaatgg
      721 tgatgatgat tgcgccttga acgcggaaat accccaatac gaccaccatg acaaaaccga
      781 acaatgccaa cagtgcgctg ggctgatgaa tatcgcccaa gccgaccaag gttgccggat
      841 tggcaacgat aatgcctgcg cctttcaggg aaatcagtgc caaaaacaaa ccgataccgg
      901 cggcaatcga cattttcaaa cccataggca gtgcgttgac cagcatttcc ctgactttaa
      961 aaaagctgaa caggatgaaa atcagaccgg aaatgaacac cgcacccaac gccacctgcc
     1021 aaggcacgcc cataccctta acgacggcaa aggtgaaata ggcattcagc cccatccccg
     1081 gggcaagcgc aatcggatag ttgccgataa aacccatgac aaaacagccg atggcggatg
     1141 cgatacaggt ggcgacgaat accgccccca tatccattcc ggtctcgccc aaaatcaggg
     1201 gattgacgat aacgatgtag cacatcgtca aaaaggtcgt caaacccgcc atcaactcgg
     1261 tacgtaccgt cgtaccgttt gccttcaggt taaaaaccct atccagcaat gtttgttttg
     1321 aaatatccat atctagcagt ccgtttcaag taaaaaatct gaacccgcac accgcaaacc
     1381 gcacaggatt cggcatattg aaaaatatac ctcccaaatc gggaaaaccg gttcgggagg
     1441 caataagttt acggcagacg gcacattatt tgtctgaatt gtgttgacga cgcaaaattg
     1501 caggaatttc aaaatcgtca aggacagact ggttatcgaa atccgctgct gtcagattca
     1561 tggtgcggat accgcgattg gtgcggatca taccttcgac attgtggctt tgctcctgtt
     1621 tggacggggc aaccgcttct acttcccttg ccggagtagg atcgaccgct cctttttctt
     1681 tcagaccggt agcgataatg gtaatccgga tggcatcttc gctcatggtc tcgtcttcag
     1741 cggcaccgaa tttgcattcc aaatcgggat gcgcgctttg gttgacgatt ttcatgactt
     1801 cagacaactc ggacattttc aggcaacccg gagcagtcgt aatattgacc agcacaccgc
     1861 gtgcaccatc caaagttaca tcgtccagca gcggactgga aatggcctgg tcggtcgcca
     1921 tacgcgcacg gtcgatacct tgggcataac ccgaacccat catagcgata ccgcggttgc
     1981 tcatcacggt tttcacgtcg gcaaagtcga ggttgatgat ttcgctcggg caagttacca
     2041 cttcggaaat gcctgcgacc gcatcgcgca atacattgtc cgccgcgcgg aaggcttcgc
     2101 gcatagttac gtcttcaccc aatgcggtca tcagtttgtc gttcgggatg ataatcagcg
     2161 aatcgacgtg ttctttcaac tgttccaacc ctgcctgtgc cacatggacg cgtttgcctt
     2221 cataagaaaa cggacgggta actacggcaa cggtcagaat gcccaaagac ttggcaatct
     2281 cagcaacaac cggcgcggaa ccggtaccgg taccgccgcc cataccggtc gtgataaaca
     2341 gcatattggc accgcgaatg gcttcttcaa tggcctcccg atcttcctgt gctgccgcac
     2401 ggccgatatc gggattcgca cccgcgccca aaccgcgtgt cagattcgta cccaactgga
     2461 ttctcttcgc cgcatggttt tttgccaaag actgcgcatc tgtattggca ctgataaact
     2521 ccacactgcg cacattgttg gcaaccatgt tattgattgc attgcaaccg ccgccgccca
     2581 agccgattac tttaattacc gcagggctga ctgccgattc tgccacgtcg taaacaaatt
     2641 ccattcaaaa actcctgatc gccccgttca gaggacggtt taaataaatt attatttatt
     2701 atataagaag tatcttactg ctggcaaaat acttctcacc tgtcaaacgg caatccaact
     2761 gttcagaggt tgttttcaat ccaccgcttc aatcttgcca acaaaccgcc gcccccttct
     2821 ctctcttgca ctgtaccgtt ttccggttgc ggcaagtttc cttccagctt gcatgctgca
     2881 tgaagcagcc cgatagcggt agaaaagcgc ggtgtgcgga cacggtcgga caaaccgccc
     2941 atttcttggg gcgcaccggt gcgtacaggc aaatcgaaga ttttttcggc aaattccaca
     3001 atcccggtca tcatggacac accgccggtc agaacgatac ccgcattcag cacttctttg
     3061 gggaaacccg atttttgcag ttcgcccagc actacgccaa aaatctcctg aatacgtgcg
     3121 ctgatgattg ctgccaaaac cttactggaa acctgacgcg atgtccggtc acccacgccc
     3181 ggaacttcaa tcatctcgcc caagccttcc gtatcgcatg atgccacgcc ataatggatt
     3241 ttaatgtact cggcggcatc gagaggtgtt ctcaacgatt tggacaaatc tttggtaatc
     3301 agattaccac cggccggaat gacggacgta tggcggatgg cgccgttcat ataaacggca
     3361 atatcggtcg ttccgccacc aatgtcaatg acgcatacgc cgaggtcttt ttcatcttca
     3421 gtcagcaccg cctgcccgct tgccaacggt tgaagcatga tctgatcgct tttcaagccg
     3481 cacagctcga tacatttttg gacattctgc actgccgtac ttgcaccggt aatgatgtgc
     3541 acccgcgtat ccagacgcac accgctcata ccgatgggct cccttacacc aagttgggtg
     3601 tcgataatgt agtcttgaac cacggcatcg agaatttttt gatcgggcgg gatattgatt
     3661 gcctttgccg tttcaatggc gcggtcgatg tctgcctgtg ttacttctcc gtctttgatt
     3721 ttaaccacac cttgcgaatt aaggctgcgg atgtggttgc ctgcgatacc tgtggtaacg
     3781 tgggtaattt tggtatccgc catcagctcg gcatcattga ccgcctgcct gatggcttgg
     3841 acggtggcat cgatattggt taccatgccc gcgcgcaagc cccgtgaagg agcctgcccc
     3901 aaaccgatga tgttgatttt gtcgtcatct tgaacttccc cgatcagtgc gaggacttta
     3961 gacgtaccga tatccagtac gctgatgtat cttttctgct gttccattgt tcgtctgccc
     4021 ttaaaactga ttgaaatttg cgtcgcaccg tttcagacgg cacggccgta atatgtccga
     4081 tacctgttcc cactattctt cggattcttt ttcgggtaaa ccgtcgggag catggcggac
     4141 tgaaaatccg tccttatacc tcatatccac ataggataac cgatttttat tcttacgcaa
     4201 cagatgctgc cacgcttcgg taaaaagccg gaggcgtttc atctcgtttt cccgtccgag
     4261 cctgacggtg atgccgttgt ccaaaacgac attccacgcc gaacgtgccg tataggtcat
     4321 ctctttgatg cccaaaccct gttttgccaa aacagtcgaa aattcgtcat aacggcggag
     4381 catttcggca gacgttcctt ccgcgcctct gaataccggc attccgggtc tgtccaagcg
     4441 ggcttcaaaa acattgcctt cgccgtccac caaggcatgg tcgccccaac gtgcaaccgg
     4501 cttgcgctcg gtcaggacga cctcaaccgt atcgggaaaa cggcggcgca ccatgaccga
     4561 cgcaatccac ggataccggc ggtaggcttc ctgtgcgcca ttgatgtccg tcctcaaaat
     4621 attcccatgg atgtattctt tcgccaaact gcccaatgcc ttcttatcgg aataaaccag
     4681 gttgcccttc agcgacacct gcttgacggg cagatgattc gaattgtaaa accaaaccag
     4741 cccggacgca gcaagcagca tcgccatcat gacaagcagc cagcgcgtca gccgttccat
     4801 cgcttcggca ttatcccaca tgtgcggcct tcaaaatttc aatacataaa tcggcaaaac
     4861 ccacgcccgt aacggcagcg gatttcggta ctaaactatg gccggtcata ccgggcaggg
     4921 tgttgatttc caacagatag agtttgccgt cggtatcttt gaggaaatcg acgcgcacgc
     4981 agccttccgc accgattgcc tgtgcgccgc gaaccgccag ttcgcgcatc aggctttctt
     5041 cggcttcggt caaatcttcc gaaggacatt gataaatggt gtcgtctcgg ttgtacttgg
     5101 cttcgtagtc gtaaaactcg gttgcgggga tgatgtgtat gccgggcagc cctttgccgt
     5161 tcaggacggg gcaggaatat tcgccgccgc cgataaaacg ttcggcaatg atttcgccct
     5221 gaaggtgttt caattcttcg taaacgcttt tcagacggcc tttttctttg acttttacca
     5281 cgccgacgct gctgccttcg gccgccggct tcacaaacat cggcagaccc aatttttctt
     5341 cgacggcatc gaaatcggta tcatcgtaca gtacggcgaa ctcgggaacg ggtaatccca
     5401 atgcctgcca aatcagtttg cagcggtatt tgtccatgcc gatggcggag gcggcgacac
     5461 cgctgccggt atagggaatg cccaacagtt ccaatgcacc ctgaacagcc ccgtcttcgc
     5521 cgtaagtacc gtgaaggatg ttgaatgccg tctgaaagcc ccgctccttc agttcggata
     5581 acggcgtttc cttagggtcg aaggcgtatg cgtctatgcc tttgcttttt aaggcgttca
     5641 aaatggcggt accgctgtcc agcgagattt ctcgttcgct ggaaaaacca cccatcaata
     5701 cggccacttt gccaaaattc tgcattgttt ttgttctttc ctaattgctt tatgcttgtt
     5761 gtcagaggtc gtctgaaacc tgattgcggt ttcagacgac ctttatatga tgttccgtct
     5821 gtcaggcggg tgtatctcaa atctgtttcg acaattccaa cagcgcggag ggaacgcggt
     5881 tgatgcttcc cgcacccata ttcaacacaa catcgccatc ctgtaaaaca ttcatcagca
     5941 tttgcggcag gtcggcgacg ttttcgcagt aaatcggctc aagtttgccc aatacgcgga
     6001 tagcacgcgc caaggcgcgg gagtcggcgg cggcaaccgg ctcttcgccg gcggcataaa
     6061 cttcggtcag taccagcgca tcaacggtat tgagtacttt ggtaaagtct tcaaacaaat
     6121 cgcgcgtgcg ggtatagcgg tgcggctgga aggcgagtac caaacgtttt tccggatacg
     6181 cgccgcgtgc agcggcaagg gttgccgcca tttcgacggg gtggtgtccg taatcgtcca
     6241 ccagcaaagc ggtcccgccg tttggcaact tgatgtcgcc gtatttttgg aagcggcggc
     6301 cgacgccttc aaagccgagc aagccttttt ggatcgcttc aaccgatgcg ccgacttcca
     6361 gcgccacgcc gatggctgcc aatgcgttca gcacgttgtg tctgccgggc atattcagca
     6421 cgacttcaaa cgacccctgc tcatgtcctt tcatttgaac atggacggtg aatttcattt
     6481 gcgcgccgac gttttcgatg tcggtggcgt agatgtcggc ggtatcgtcc aaaccgtaag
     6541 tagcataagg tttgctcact ttgggcaaaa tcgcgcggac gtgttcgctg tcaacacaca
     6601 aaaaggcttt gccgtagaag ggcatacggt ggatgaaatc gataaacgcc tgatgcagtt
     6661 tttcgacgct gtgcccgtag gtatccatat ggtcttcgtc gatattggta acgacggaca
     6721 taatcggggt cagatgtagg aaagaggcat cggattcgtc ggcttcggca acgatgtatt
     6781 cgcctttgcc caagcgggcg ttggtgcctg cggcgttgag tttgccgccg ataacgaaag
     6841 tggggtcgag tcctgccgcg ccgaggatgg aggcggtcag gctggtggtc gtggttttgc
     6901 cgtgcgtacc ggcaatggcg atgccgtcac ggaagcgcat cagctctgcc agcatcaagg
     6961 cgcgcggaat aacgggaatt tgccgctcca acgcagcgac aacttcggga ttttctttct
     7021 tgacggcggt agaggcaacg acgacatccg caccgttaac gtgttctgcg gtatggccgg
     7081 gataaacttg aatgcccagg ctgctcaaat gctcggtagc ggcatttcgc gcctgatccg
     7141 aaccggaaac tttaaagccc aaattgtgca agacttcggc gataccgctc atgccgacgc
     7201 cgccgatacc gacaaaatgg atgttgctta ctcgattttt catcataatg ttgcgttccg
     7261 gttggttttt agtgcgtaaa ggcgttattt taaaggtctg accgtttgcg caccatagtt
     7321 ttctgacaaa tatatagcgg attgaaataa aaaacatcca tgccgtctga acggcttttc
     7381 agacggcatg gttcggcagt ttacgccgca cacgcaatcg cggcttcggc aacgtcatcc
     7441 gcgctgtgcg gcaatgccaa cgtacgggcg ttttccgccc atttgaggca tttttcgcgg
     7501 ttgaggctgc cgaggatttc ggcgagtttt tccgccgtca actgggtttg cggcaacagc
     7561 agccccgctt ctgcctgcac catgaaacgc gcgttggcgg tttgatggtc atcaacggcg
     7621 tgaggatacg gcactaacaa cgcgcccagc cccgccgccg tcaactcggc aatcgtcagc
     7681 gcgccggcac ggcaaatcac caaatcggca tcacggtagg cggacaccat gtcggtaata
     7741 aattccacgc attccgcttt cacgcccaac gcgtcataat ccgcctgaag attgcccagc
     7801 ttgttacgcc ccgactggtg gtacatctgc gggcgtacct cttcaggcag cagtgccaac
     7861 gcctgcggta cggttttgtt caaaacgtcc gcacccaaac tgccgccgac caccaaaatt
     7921 ttcagacggc cttcgcgccc ttggaagcgt tcggcaggca cgggcaggtt gctaatatcg
     7981 gcgcggacgg ggttgccgac caaaccgcct tcgtggctga acgctttcgg aaaagcgtac
     8041 aacacccgtt tcgcccagcg cgacaggtgg cggttggaca agcctgccac ggcgttttgc
     8101 tcgtgaatca caatcggcac gcccaagagt ttcgccgcca gaccgccggg aaaggtaaca
     8161 aaaccgccga agccgatgac gcactcgaca cggtgtttgc ggataatccg ctgcgcttcg
     8221 cggacggttt tgtacagagt aaacggaagc atcagcttgc gtttgatgcc gttgccgcgt
     8281 attcctttaa tcgccagcgt ttccaagcgt atgccgtatt gcggcacgat gcgctcttcc
     8341 atcgaatcct tgctgcccag ccaaattaca tgatgaccgc gcacgcgcaa tgaatccgcc
     8401 acagccagag ctgggaaaat gtgtccgccc gttccgcccg ccatcagcat aaaggtttta
     8461 ccgcccatga tttactccac ccggtaaccg cgcattttcc ggcggttttc ataatcgata
     8521 cgcaacagca gcatcatgct gatcagcatg aaaaagactg acgaaccgcc ataggacatc
     8581 aacggcagcg tcagaccttt ggtcggcaaa gcaccgatgt tcacaccgat attgaagaaa
     8641 ctttggatac cgatccaaat gccgataccc gaagcgatat aggcgttgaa agtcaaaccc
     8701 aaatcgcgcg actgcttgcc gatggaaaac gcccgcacca ccagccagcc gtaacagaat
     8761 atcagcacgc acatcccgaa gaagccgaat tcttcagcga tgatggcaaa aataaaatcg
     8821 gtatgcgctt ccggcagaaa gccgcgtttg ctcaaactcg cacccaaacc cataccgaac
     8881 cactctccgc gcccgattgc catcagagag tgggtaagct ggtagccggc accctgcggg
     8941 tctttccacg ggtccaaaaa tgccactacc cgctgcacac ggtagggagc ggcggtaatc
     9001 atcagcacca tcccacccaa gacgctgcct accaggacaa aaaaatattt ccacggcaat
     9061 cctgccagaa acagcattcc aacggtaatg acggtaatga cgacaaacga accgaaatcc
     9121 ggctgtacca ttatcagcac caaaccgaac gccaccagca taatcggcag gatgatcgcc
     9181 cggaaacggc cgtacatttc taatgtttca cgacgtgcct gcggattggt ggcggacata
     9241 atcaggttgg ccgtcccccg ccaaatcgac tgccaaccca aactttccat gctgcgcaac
     9301 acttcttcac ggcgcgtgaa caggcttgcc aaataaagga tgactgccag cttgaacagc
     9361 tcggtcggct ggaaattcaa cggacccaaa ggtatccaac gggtcgcgcc attgatttcg
     9421 cgcccggcaa tcaatacggc taccagcaac aggccggata aggcaaaaat ccacggcaca
     9481 agccgccgcc atgtcctcat cctgcaaaga aaccataaaa aaccgctcgc tataaggccg
     9541 gcaacgacga accccgcctg cctggtcaaa tagaaaaact gatcgccgcc ttccttcgat
     9601 gccaaataca cagaagccga ataaatcatc agcaggccga acgccgtcat cagcaccacc
     9661 atccacaaaa gcggcgcgtc gaatttcctg ccgtcgcgca caatcggcct gtcgagcagc
     9721 agagtgtgga caccgtcgcc cacttttacc aatacttccg aaatcttcaa aaaaccacct
     9781 gccagtctgt ttgcacctgc cgcaaagggc aaaaatttca gacggcagac aatgccgtcc
     9841 gaacatacga tacatcccaa atcggtattc taaatcttta cttgccgccc aacaatgacg
     9901 gcgtttgtgt ttcggacggc atcacaaagc cttaaacgct tcgataaaca cttccgaacg
     9961 gtgcgcgtag cctttaaaca tatcgaaact cgcgcaggcg gggctgagca agacaatatc
    10021 gcccgcttcg gcttgggcgt atgccgtctg aaccgcctct tccaaagtga cgcagtcggt
    10081 caggttcaag ccgcagccgt ccaaatcgcg gcggatttgc ggcgcatcga cgccgatcag
    10141 gaacacgcct tttgccttat ctttcaacgc gtcgcgcagg ggcgtgaagt cctgcccttt
    10201 gcccatgccg cccaaaatca cgaagagcgg gttttgcaaa ccggcaatcg cggcggcggt
    10261 cgcgccgaca ttcgtgcctt tgctgtcgtc gatgaatacc acgccgtttt tctcgccgat
    10321 tttttccaca cggtgcggca agccttggaa ggttttgacg tgttccagca atgcttcgcg
    10381 cggcaaaccg acggcctcgc acaaagccac ggcagccata acgttggcgg cgttgtgcaa
    10441 accttgcagc gggatgtctt gcgtagaaat caaatcttca ttgccttgtt ttaaacagcc
    10501 cgtcccgcgt tccaaccaaa aatcggcttc gtgttccaag gaaaaccgtt tcacttcacg
    10561 ccctgcccgt ttcatggcgc ggcagaacac gtcgtccgca ttcaaaacct gcactccatc
    10621 gccacggaaa atcttggctt tggtatgcgc gtagtcgagc aaatcgtcgt agcggtcgag
    10681 atggtcttcg gaaatgttca tcaccgtcgc cgcagtcggg cgcaggcttt cggtgttttc
    10741 cagttggaag ctggaaagct ccaacaccca cacgtccgcc tttttgcctt cgcgctgcaa
    10801 ttctgcctcc aaaaccggcg taccgatatt gcccgcaatg acggtatcca gcccgcactt
    10861 gatgcagaga tagccgacca ggctcgttac cgtggttttg ccgttgctgc cggtaatcgc
    10921 aatcaccttg tcgccgcggc ggttcacaat gtccgccagc aattcgatgt cgcctagcac
    10981 gcgcccgccg ttttgcttga acgcctcgat atccggctgc cgctcgctga tgccgggact
    11041 gagcgccaga atatcgaaac cgttgtccaa cgcatctttc agacgacccg tgtaaaacac
    11101 cagcccgtca aacatcttac cgatttgcgc cacgcgctcg gctttcagct ccgcatcata
    11161 agcggcaacc tccgcgccgt ttttgcgcag ataggcaatc atggaaatac ccgtgccgcc
    11221 aagtccggcg acgaggattt ttttgttttg gaaagtcatt ttggttttgt cctaaaacaa
    11281 atcatattgg gcaggagacg tccgcccttg cccaagccgc tttcagacgg catcgcgagc
    11341 cgattaataa cccgccttca ggcgttggtc attgtcgcag ctgttttggt ctccgttttg
    11401 acaagccttg ccaagccatt gttgagcgag cgcaaggtct tggcgcacgc cgcgtccatc
    11461 gtaatacatc aagcccaaat tgtattgggc ttgggcatcc ccttgttctg aagccttgcg
    11521 ataccactgc actgcctgta cataatcttg acgaactcct tgtccatttt catacatcac
    11581 gcccaaattg aattgggctg ctgcatttcc ctgttctgcc gcctgcaagt tttcccgaaa
    11641 atccgatacg tcacccgccc acaccgcttg gttcaagccc aaggcaatca gggcggcggc
    11701 aagccatttg actgtctgtt tcatggtttt ttcctgtttt agtataaggc gggtttcagc
    11761 cgccgttaac gataggacgg ggcggattgc cgccgcaggt ttattgcgcg ttcaaatgcc
    11821 gtctgaaagg tgttcggacg gcataggttc agcgaatttt gagggtactc aaaccgatca
    11881 ataccaagac gatggtaata atccagaaac ggacgacgac ttgggtttct ttccagcctt
    11941 tttgttcgta atggtgatga atcggcgcca tcaggaagat gcgttttttg gttttcttgt
    12001 accagccgac ctgaagcata acggacacgg cttctacgac gaacagaccg cccataatga
    12061 cgaggacaaa ttcttggcgg acgatgacgg caacggtacc gagcgcggca cccaatgcca
    12121 gcgcgccgac atcgcccata aagacttgcg cgggataggc gttgaaccac aaaaatccga
    12181 ggcacgcgcc gcacatggcg gtgcagaata tagcgacttc gttcgcgccg gcgacatagg
    12241 gaagctggag gtattgggaa aattggtagt gtccgctgac gtaggcgaaa atggcgagcc
    12301 cggcggcaac gaggacgacg gggaaggcgg caaggccgtc caagccgtcg gtgaggttga
    12361 cggcgttgga tgtgccgacg atggtcaggt aagacaacac caaaaagccg accacgccca
    12421 gcggcagggc gatttgtttg aaaaacggga caatcaaaat attgttggcg gaattggcgg
    12481 caaggtaaaa caatgccaaa ccggcgataa cggcaacgct tgactgccac accattttga
    12541 atttggcgga cacgccgttg gggtctttat aaacgacttt gcgccagtcg tcgtaaaaac
    12601 cgagcgcacc ggtggcaagc agtacgccca agagaatcca gatatacggg ttcgcccagt
    12661 tgccccacaa cagggtggac acggtaatgg cggtcagaat cagcgaaccg cccatcgtcg
    12721 gcgtgccgtt tttgacgagg tgggtttgcg ggccgtcggt acgcactgcc tgcccgcatt
    12781 tgagcgcggt cagcctgcgt atcgtccacg ggccgaacat cagggaaaac gccaaggcgg
    12841 tcaacgccgc cataacggcg cggaatgtgg tgtattgaaa aatattcaga ccggttaacc
    12901 agttgctgaa atgtgcgagc cataaaaaca tggggcttcc ttttttgttt tgtcgttata
    12961 gcggattaac aaaaaccggt acgtcgttgc cccgccccgg ctcaaaggga acggttccct
    13021 aaggcgccca agcaccgggc gaaccggttc cgtactatcc gtactgtctg cggcccgccg
    13081 ccttgtcctg atttttgtta atccgctata catcgggctt gcgcccgctt gaggtttgct
    13141 tgctgtccgg caaggtttcg gacggctttt gcagattaac gtgcagggac tttcccaatc
    13201 acgcaaatcg ggtagctccc cggatttttg ccgccgccca agtctaaaca taaatcttca
    13261 taaagatacg cctgcgtccg cattccggca taaaacgcgc cgaccagcgc ggcaatcagc
    13321 aataaggctt taaaaaaacg tcggcttttc attttcactt atcctccaat gcctcgacca
    13381 cttcttccat ctgcataaag cgcgaacctt tcaccaacac ggtggcgcgt tcgggcaaat
    13441 cgtggctcaa cacttgaatc aacgggtctt tggcggcgaa ccacaaaccg tccgcgccaa
    13501 atttttccgc cgcttcgacg ctgttgtcgc cgacaaaata agccgcttcg atgccttggt
    13561 cgcgggcgta cgcgccgact tcggcgtgca tggcggcggc ttcgtcctcg cccagttcgc
    13621 ccatatcgcc catcacgaaa atgcgcggcg caggcatacg cgccaacacg tcaaccgcgg
    13681 ctttcatact gtcgggattc gcattataag tatcgtcaat cagggttgcg cccttgatgc
    13741 cggctttgac gttcagacgg cctttgatgt tgctgaagcc ttgcaaacct tccgccacat
    13801 cgttcaaact caaaccggca gccaaagcca gcgcggcggc agcggcggcg ttgtggacat
    13861 tgtggcggcc gggaacaggc agcaccacgg cagtgcgctc gtcgccgcac accaaatcaa
    13921 attcgcacga caaaggtttc agcacgatat tttccgcgcg gacatcgccg ctatcgacgc
    13981 cgaaagtgca cgtattcaaa ttaaacgttg ccgttttgaa gacagccata ttggcatctt
    14041 cttgaggaat cagtgccatg ccgtctgaac ataagcctgc ataaatctcg cttttcgctt
    14101 tggcaatatc gcccactccg tcgaaaccgc atccgacatg ggcgcgcagg gcgttgttga
    14161 ccaaagcggc atcgggtttg gcgatttgcg ttaaaaccgc cagttcgcca aaatggttca
    14221 tgcccatttc aatcacggca tagcggtgtt tttcgtttaa tttcaataaa gtcagcggca
    14281 atccgatgtg gttgttgaag ttgcctgccg tcgccgaaac ggcatcatcg ccgaaacggc
    14341 ggcgcaatac cgcagccagc atctccttca ccgtcgtctt gccgcccgaa ccggtaatgc
    14401 cgaacacaaa cgggttcaca ttatcgcgcc acgccttcgc caacgtttgc aacgcggcaa
    14461 gcgtgtcatc gactttcaac gcgccgccca aagccgcgca atcttcgcgc gaaaccacaa
    14521 ccgccgccgc gcccgcagac aatacgcctc caacaaaatc atgcgcgtca aaccgcccgc
    14581 ccgccaatgc gaaaaacaca tcgccttccc gaatatcgcg gctgtcggtt acgatgcgcg
    14641 acacgggttt gttttcagac ggcatcggaa gcttgagggc ttggcagatg aaatttaggt
    14701 ccagtggttt catatttgct ttcgttaata ttcgggcggc ggacacatcg gtagcggctg
    14761 atttttttat cgcctgtttt gctgtggtaa aacacagatt attttcccat tctcattcgg
    14821 cattttttct gtacgtatca ttttttagac gtatttttag ccgatttgcc ttttcccgca
    14881 taccacggcg cggggtcgtc ggactgtctg tcgataaagg caaggttatt gccttcgccc
    14941 ggcacatcgg ggacattccc ccaaaaatca tagccgtcat cgggcaactc gtcggtttcg
    15001 atacccgtcc aactgccgaa tccgcgtaaa aaattaaacg cgctgcgcct cactttaatc
    15061 atttcgcgcc gcgtgtcatc ggacgaaatt ttaaccagcg gcacgccgta gctttgacgc
    15121 ttccactcgc cgtgtgtcaa cgttcgctcg ccgcctttgc cgacatgcat caaaccgtgg
    15181 tcggaaaagt aaaccaaaga ccagctttct ttattttcat tcaatatctt aaccgtatct
    15241 tctaaaaatt tatcggtttg cgcgatggtg gaaacatagc aggatatttt ttcagtttga
    15301 tactgaaacc gccgcgcatc cttatccaaa cgtgtgcaaa aatcactgtg cgaacccatc
    15361 aggtgcatca caatcagccg aggcttcgtg cctgcatttc ctgtcaaaac ccgtttgaac
    15421 gccggcaaca aaaggcggtc gctcaatccc gcgcttttgc cataatcacc cctttgggta
    15481 aaccacggat aatcgctgcg tagggcatag gtggaaattt cgttggcaaa atgccccaac
    15541 attccttgat tagacagcca cgccgtccga aaaccagcct gcttcgccaa gctgacaatg
    15601 ttattgttcg gttctcccgg cagccccaaa gtctgcggca gcgaaagatt cgtcgcgtgg
    15661 gcggtcgatt ggtaaccgtt tatcaacagc cctttggtcc gactcaaaaa aggcgtatcg
    15721 ggcaatggga aaccgtaaac attcatataa tccgaacgcg cgctctcacc gataaccaca
    15781 acataatttt tatacttggg cgcaacatga cggatatgcc atgttgacgc tttttttgct
    15841 gcttccaaaa tgtgggcgcg cttggcggca tattgtgccg gcgcggaagc caaatcgtaa
    15901 tacaggtcga aaatattcaa caacaggccg gcatcaggtt ctcgccaatc tttatcgccg
    15961 gcgattttct ccatcaccgc gcaggataaa atcaaagtca ataatatggt cagccatatt
    16021 ttgctgcggc gtttatagtt ttttacgtca gcaaataccc ccacagatac acaatatttc
    16081 caaactgtca agccaaaaat gaaaaataat gcctgcacaa aataaagcga cccgggaaga
    16141 ttgccgacaa attcacgcgc ctcggcagga ttgctttcca atatcgaacc gactatctga
    16201 taagaaggcg caccatacag ccagccgacc ggcaaatata gggcggttgt gccgacgtaa
    16261 atcagtaaaa caactgaaga aacgcgcggg aagccgcgtg ccaataataa taaaaataca
    16321 accgaagcca aagcgcccac ggcagaataa cggtagccgt attcatattc caagtgataa
    16381 cctgatgcga tggccgcgcc aaaacaaaag gctaaaagcg cgcaaatgcc caatgtattt
    16441 tttaatgtct gatccatatt ttttattcgg gcggattgtt tcatacgatg ccgtccgaat
    16501 atcccattat ttacgagtta acaaagcctg cccgacgatt tcaagatcgg aaaaacggtg
    16561 cttcacgccc tgtacatcct gatagttttc gtgtcctttg ccggcaatca ggatgatgtc
    16621 gtttgcggcg gcctgctcaa ccgcataacg gatggcggcg gcacggtcgg cttcgacgca
    16681 ttcgggcgcg ggcacggcgg gcaggatgtc gttgatgatg tcttgcggat tttccaaacg
    16741 cgggttgtcg ctggttacga cgactttatc cgcgccctgt acggctgccg cgcccatcaa
    16801 tgggcgtttg ccgcaatcgc ggttgccgcc gcaaccgaat acgcaccata aggccgcacc
    16861 ctgcggtttg atttcctgca aagtggagag tgctttttcc aatgcgtcgg gcgtgtgggc
    16921 gtaatcgacg acgaccaagg gcttgccgct gttcatgatg cagtccatgc gccctgaagc
    16981 gggacggatt tttgccagca catccaatac cttatcaagc ggataaccgt tggcacaaag
    17041 caaggcgatg caggcggcga ggttttgcgc gttgaaccgt ccgagcaggc gggtgcggca
    17101 ttttccttcg ccccacgggg tttggaatac tgcttccatg ccgtctgaag aggcggtaaa
    17161 gtcggtaatg cggatgtcgg cgtgttcgct gaaaccatag ctgtaaacgg ctaaatcggg
    17221 acagtctttt ttcagacgac ctgcgagttc cgcgccgtat ccgtcatcgg tattgataac
    17281 ggcgtgtttc aagccgtgcc agtaaaacag gcgcgacttg atagcaccgt aggcttccat
    17341 cgtgccgtga tagtcgaggt ggtctcgggt gaggttggta aagattgcgc tgcggaatgg
    17401 cacgccgttg acgcgcgact ggtcaagacc gtggctggag acttccatcg cggcggctgt
    17461 tgcgccctgt tggcggaaac ggtagagcag ggtttggaca tcgacggggg cgggggtggt
    17521 gtgcgtggtt tcttccaatg cgccccaaaa gccgttgccg accgtgccga taatggcggt
    17581 tttttcgccc aataaatcgg cagcctgcgc cagccattgt gtgatggagg ttttgccgtt
    17641 ggtgccggtt acgccccaga ctttgaggcc gtctgaaacg ttgtcgtaca cttgcgccgc
    17701 caacatgccg gcacggtgtt tcaaattttt gatgccttga ttggggactt tccattcggg
    17761 attccacgcg aatttgccgt cgtcgtccca aaaaacaaaa gccgcgccgt tgacaacggc
    17821 ggcggggata taactgcggc cgtcggtata ttcgccctga caggcaacga aaatatcacc
    17881 ttgtttgatt tgacggctgt ctgaatgcaa caaacgccct gctgcgtttg cacacagcag
    17941 aggcggaatg ttggtttcag ctaaaggggt taacttgctg aacataaaat aatctcgttg
    18001 atactcggat taagacggtg ttttgacggc tgcaacattg gtcaaaggtt tggtcgggga
    18061 aacgcccaag atgttcaggc tgccgcccat aatttttttg aagggcggcc ctgccactac
    18121 gccgccgtaa tagctgtggg cagtcggttc gtcgatggtt accgccacaa tcacacgggg
    18181 gtttttggcg ggggcaaaac cgataaacgt accgacgtgt ttgttgtcca catagcggcc
    18241 attgaccagt ttgcgcgccg taccggtttt agcgccgaca tcgaaaccgt ccaccgcacc
    18301 cgccgtaccg gtgccgcccg gctcggttac ggaaaccatc agattgcgta cctcgcgcgc
    18361 ggtcgattct ttgaatatgc gtttgccttg cggcgcaacc gcctgcttct caaagctgag
    18421 cggcagcaaa acgccgtcgt gcgtcagtgc ggtataggcg cgcgccaatt gcagcaggct
    18481 caattgcagg ccgtaaccga aagacatcgt cgcctgttcg atgggccgcc acctgcgcca
    18541 atttctcaac aaacctgcag tttcccccgg aaagcccgag tgcatacgca caccgatgcc
    18601 caattcatga tagaagtcat acatttcttc ggcgccgaaa cgcgcagaca gtttgcttgt
    18661 gccgacgttg gacgatttct gcataatgcc gcgcacatcc aaagaggggt aaacatgggt
    18721 atcgtcgcgc acgggagacg gtccgatttt ataaggctgc gtattcagcc gttcgttcaa
    18781 atcggttttg cccgcatcca atgccttcgc aatcacgaac ggtttgattg ccgaaccagg
    18841 ttcgatcata tcggttacgg cacggttgcg ccgctgttcg ctgtctgccc ggccgggtct
    18901 gttgggatcg taggcgggcg tattggccaa ggcgaggatt tcccccgtgc gggcatccaa
    18961 aaccaccacc gttccggctt ttgcctgatg gtattcgacc gccttgttca actcttcata
    19021 ggccaaggtc tgaatcctct gatcgaggga aaggatgatg tctttgccgt tttgcggtgc
    19081 tttattgcgc ggggagtcca agctgtccac aatattgccc tgccggtccc gcaaaacaac
    19141 ttccgcgccg tcttcgccat acaggctgtc ttcaagcgaa agttccaaac cttcctgacc
    19201 tttgccgtca atatcggtaa atccgatgac gtgtgcaaac aggttgccca tcgggtaatg
    19261 gcgttttaat tctttttcaa atacaaagtt ttccaaaccc aaggctttga cctcttcggc
    19321 aaccttggga tcgagctgcc gcttgatcca aataaacgac ttgcctttct gttcgagttt
    19381 gttcctcaaa acatcgaccg gcacatcgac aagctcggac aggcgttcca attgggcggc
    19441 agacggcatt tccttcatat ctttaggcac ggcaaacagg gactccgtcg gcgcgctcaa
    19501 cgccaaaacc gcaccgttcc ggtccgaaac cgtaccgcgt gtagccggca atgcttgagt
    19561 ccgcacaatc cggttgtcgc cctgttcttt caaaaagtta tacgttaccg tctgcagata
    19621 cagcccgcgg gcaatcagac aggcaaacaa gaccgccatt gccatcagga cgaagctaat
    19681 ccgtccgtta ctggtcatcg gctttttgac ctgctcttct ttgggcagca tccggggctt
    19741 atattcgctt ttaatcaaca tttttacttc tcgttattat tatcctgatg caggaatccg
    19801 attccggcac acaggctgct tctatctttg atgttccacc ataaaggtat tgcccgaaac
    19861 cggcggatgg aggttttgtt tttctgccgc cgccctgatc gcttcgtgat tcgccaaacg
    19921 cgcctgttgc accctcattt gcgcataatc ctgctccaag gcgatttcct gttttttcgc
    19981 cttgtccaaa gctgtgaagt tgagcctgta ctggttttgc tgcatcacaa cggaaaaagc
    20041 ggaaacgcac accgcaagca gcagaaagaa attcgatttg ttcattgccg ttcagacgtt
    20101 tttctctgtt attattccgg tatcggaccg gcagtccgct ccgccacacg caaaactgcg
    20161 ctcctcgccc tcgggttggc ggcaatttcc gcttcacccg gctttaatgc cctgcccacg
    20221 attttcaggg gcggctcggg caaatccgct tctctgaccg ccgcccagct cggcaggggc
    20281 tcgtgttgcg aatatttttt gacaaactgc ttcacaatgc ggtcttccaa cgaatggaaa
    20341 gcaatgaccg ccaaacgccc gccctctttc agacggcaca tgacctgcgg caataccgcc
    20401 cctacttctt caagctcgcg gttaataaag atgcggattg cctggaaggt gcgcgtcgca
    20461 ggatcctgcc cccgctcgcg agtacggacg ttttgtgcca cgatctgcgc cagcttgcgg
    20521 gttgtatcga ttggactttc cgcccgttgc gcgacaatgg cgcgcacaat ctggcggcta
    20581 aaccgctctt caccataatt cttgattacc tcgtgcaaat cctgttccga cgcaaccgct
    20641 atccactctg cggcagacat accgcgcgtc gtatccatac gcatatccaa aggggcatcg
    20701 aaacggaagc tgaaaccgcg gctgccgtca tcgatttgcg gagacgaaat ccccaaatca
    20761 aacagcgcac cgtccacctt gccgataccc aaaccgtcca atgccgtctg aaacgaagca
    20821 aaaccgccat gcacgacacc gacccgtttg tccgaacgcg ccagctcttc tgccacagca
    20881 atcgcctgcg ggtctttgtc gaaaacaatc aaccgccccg catcgcccaa acgcgacaaa
    20941 atcagccggg aatgccctcc cctgccgaac gtaccgtcca catagacacc gtcttcgcgc
    21001 acggcaagcg catccaccgc ctcattcagc aagaccgtga tatgccggta actttctgct
    21061 ccactcacaa ttgcaaatcc gtctgactca actggaaggc aagttcgtca ggatcgtcat
    21121 ccaaagcctg aaccatctca gcctcccact gctcgcgacc ccaaagctcc aaacggttgg
    21181 cacggccgac caaaacaact tcacggtcga aatccaccct tttcctcagt ccggcaggaa
    21241 ccagcaccct gccggcgctg tcccattcca aaatttccgc gttatgcagc aaaagatttt
    21301 gaaaccgccg caaaacaggg ttatccgcca cttttaagtt taaaagttgc gccgcaacct
    21361 tttcccactc cgcaacaggg tacatcaaca gcttatgttt cgactcgagc gttgccacta
    21421 cggcaggcgt atagaggcgc gacagaatgt cgcggaattt ggcaggaaca gccaaccgcc
    21481 ccttactgtc gatgcttaat tcgtgtgcgc cgccgaacat gacatgtccc aagccgaaat
    21541 caaaatcaca agggtaaaag agacactttg ccccacaatt ccccaccaat cgacactata
    21601 agaaatttta aacactcggt caaatcaggg catgaaaact cattaacata tctgaaattt
    21661 ttattctgtt taaaaacaat aagataaaaa atgacgacaa cggcacggcg ggtgcagtac
    21721 agagtgaccg aaccaataaa caactatata tgattaattt aataatataa acacaatata
    21781 tagtattaag ataaagccat gacagcaccc gtaccaacgt gtaatatgtc gggaaatcca
    21841 ataaatttac acaagctaac atttatcatg ctcctcccct cccccgaagc acggcaatcc
    21901 tcgctcaatc tgcaaaccct cattgccgaa gaaatcggca aacacggcaa ttggattcca
    21961 ttttcacgtt ttatggaatt ggttttatac gctccgcaat acggctacta caccggcggc
    22021 agccataaaa tcggcaatac cggggatttt attaccgcac cgaccctcac ccctctgttt
    22081 gcacagacac tggcacgcca acttcaagaa ctcctgcccc aaacggcggg caatatctat
    22141 gaattcggcg cgggaaccgg acagcttgcc gccgatttgt tgggcagcgt ttcagacagc
    22201 atcaactgtt actatattat tgaaatatcg ccggagctgg cagcacgtca gaaaaacctg
    22261 attcaagccc gtgcaccgga agcatctcaa aaagttgtcc acttgaccgc acttcccgaa
    22321 gcgtttgacg gcatcatcat cggcaacgaa ttactcgatg ccatacctgt cgaaatcgtc
    22381 cgtaaaaatg aaggaggatt actcgagcac atcggcgttt gcacggataa cggccgtttc
    22441 gcctattctg caagaccctt gcacgacccg tcgctttcca cctcggcttc cctctatttt
    22501 cctcaaacag attatcccta taccagcgaa ctgcacccgc aacaatatgc ctttatccgc
    22561 acccttgcct caaggcttga acgcgggggt atgatattca tcgactacgg ttttgatgcg
    22621 gcgcagtatt accacccgca acgcaatcaa ggcacattga tcggacacta ccgacaccac
    22681 gttatccaca atcctttcga cttcatcggg ttggcagatc tgaccgccca cgtcaacttt
    22741 accgacattg cccaagccgg tacggatgca ggactggatt tgacaggcta tctgccccag
    22801 tcccattttc tgctgaatct gggcataacc gaactgttgg cacagacggg taaaacagat
    22861 tcggcagcct atatccgtga agccgccgct gttcaaaaac tgattgacca gcatgaaatg
    22921 ggcgaactgt ttaaagtcat cgcattcgga aaaaatatcg gcatcgactg ggcaggattc
    22981 cgcttcggcg acatctgcca caaactccaa ccctcgtgcc gtctgaatcc gcttcagacg
    23041 gcataaactt tttaacattt aaaaacaaga aactaattca aaattaaaaa atacggcttg
    23101 tcaaaaaaac aaaaaaacat ataatagcta attcacgaaa cggcgaatta gctcagtcgg
    23161 ttagagcaga ggaatcataa tccttgtgtc cggggttcga gtccctgatt cgctaccaga
    23221 ttttcggggg tatagctcag ttggtagagc gcttgcatgg catgcaagag gtcagcggtt
    23281 cgatcccgct tacctccacc agataaaaaa gcacagaccg taaaaggtat gtgctttttt
    23341 attgcctgat tgccggaaaa taaagaataa accactgcct tcaaaacagg caatcgactt
    23401 taaacctatt gccccgcctg tcctgatttt atcagcccgc cagacaaacc cgacctgaat
    23461 aatgtcttca ggtcgggttt atggtttcat tcccaactta tccagcccga caaccacaat
    23521 attatgatga caatgccgaa aacaatgcgg taataggcaa acgggatata gtttttcttg
    23581 gaaacaaact tcagcagtgc tttaaccgct accaaaccgg aaacaaaagc ggcaataaag
    23641 cctatcaaaa tcaaaccgac atcatgcagg gtgaaaaatc ggtaatgttt caggacatca
    23701 taagccgttg ctgcaaccat catcggaacg gccaagaaaa atgaaaactc cgttgccgtt
    23761 ttccgctcga ttccccaaaa catcccgccc ataaccgtac tgcccgaacg ggacgtaccc
    23821 ggaaccagtg caaacacttg ggcaacaccg atcatcaacg catcaatcgg acgcaatgca
    23881 tcaacatcgg caattttagg ctctgctcgg ctttggcgtt tctccaccca caaaataaaa
    23941 aaaccgccca aaaccagcat gactgcaaca ctcaaggggt taaacagata ctctttgatt
    24001 tgtttgtcga acaacagccc catcacggcg gcaggtataa aagcaatggc aagattgagg
    24061 acgaaacggt tggctttccg gtcttttccc acgccatgca acacattgct gaaacgctgc
    24121 cggtattcaa acactaccgc caaaaccgca ccgagctgga tggcaatttc aaaaaccttg
    24181 tgattgctgt gaaaaccaat cagattgccg aacacaatca aatgtccggt gctggaaatc
    24241 ggtaaaaatt cggtaaaacc ttctaccaag cccatcatca gggctttcag gacaatcaga
    24301 aaatccattg cttgcgcttc tttcggatac gggagttcgg ctatttctgt acagcagggg
    24361 tctgacgctt gcgttcttcc ctgactttgg caaccaattc tttaatcgta tgaacgccgc
    24421 catcaaagcc attattgaag ataacgcggt attttccgcc gacaataacg gtcggcgtgc
    24481 cgtcaatacc gtattgttcc gtcagtttct gcatttttaa tgcgacggcg gcagcttcgg
    24541 gggaatcata ggcgcgcatc agttttttgc cgtcaaaacc tttttgagat aaagcccatt
    24601 ttccggcaac agccctgttt tccaaacgga ttttttgttc gtaaactgct ttaaacacag
    24661 cagagtttgc ctgatatttc aaacccgaca gcttgaccgc agcagccatt cttgccagac
    24721 cgagcatttc aggccgccag accacgtgct ccgtccgcag ataagtatca gacggcaatg
    24781 ccttgcccag tttcaataac aaaggatcga aatgatggca atgtacgcaa aaatagccga
    24841 aaaattccaa aacctcaatt tttcccggct gttcttgagg aatgggttta tccaacacaa
    24901 gatagtcttc cccttccgtc agggcatatg cctgcgcgga caacactgcc gacagcagca
    24961 gcggcaacag atgtttaaac ttcataatta tttgccttca atcgcacgga tcaggctggc
    25021 aaccccatgc tttttcaact cgtcctgcat ttttttcacc gcatcggcag acatattgcc
    25081 gctttgcacg cggtaaagcg ttttatgtcc cgcctgatag ccgaccactt cggaagatat
    25141 gcccaagatt gccagtttgg cacgctgccc ttccgcgctc cggcggtcgg catacgcgcc
    25201 catttgcaga taatgcgttg cttccgcctt gccaaacgtt ttcattttct gcacttcttt
    25261 ggcagcggca ctgcgcgctt tttcgatgct gccgctgttg aggatttgtt ccggggtcgg
    25321 tttgggtgca actttttcct tcgccgcctt tttctcttct tttgaagctt ttttctctgt
    25381 ttctttagac ggttttaccg cttgtttttt aaccgtttcg gcatctttct tccgcgcttt
    25441 ttccctgacg gtttgttcac gctcttccgt cagtgctttc ttgcgcactg cctgtccgtc
    25501 cggctcttcc cgttccggct cgcccgcctt ttcttcaacc tcgtcggctt tgtcggcaac
    25561 gggctgcttg tcggcagctt tttccgcatc cgactgctct gcctctttcg caacatccgg
    25621 ttcggacaag gcgttttgat cggccggttc aggttggatg tcttccttag gctggttttt
    25681 cagtttcagg atttccgttt ctgcaggctg cttcgacgga gccgggattt tgaacgcatt
    25741 ttgaccgccc tggttcagat aaagcaaaat accggcaata atgaccgttg ccagtatcaa
    25801 accgaagaag aaaccggaca gaccttttcc ggattgggaa aatttgttca taaacatacc
    25861 ttaatgtgtt tcagacggca ttaacgccgt ttgcttacgg gcggatattc taacaatatc
    25921 gccatatttg ggcaaaacct gcttccattc ccgttcctat aaagcacgac ggaagcctat
    25981 ggctttcatg acgtggcttc tgccgacttc ttcatcgccc gccaaatccg ccaatgtacg
    26041 cgccacgcgc ataatgcggt ggaagctgcg ggcggaaaga gacaattttt ccagcaggct
    26101 acccaaggct tcctgcgctt ctttttggat gcgggcttgg gaatcgagtt cgctgacgct
    26161 caaagcggcg ttgactttgc cctgccgtgc gtattgtttg ccgcgggcgg cgatgacgcg
    26221 ttccaaaacg gacgcactgc tttcgcccgc ttcctgctgc atcagttcgg cggcggacag
    26281 gctggggact tcgatggtca aatcgatgcg gtcgagcagc ggaccggaaa ttttgcttcg
    26341 gtaacgcgcg acactttcgg gcgtgcagcg gcagggtttg acgggatgcc cgagataacc
    26401 gcacggacac gggttcatgg cagcgacaag ctggaatttg gcgggataga cggcttggcg
    26461 cgccgcgcgg gaaatgtgga tttcgccgtt ttccaacggt tcgcgcaaaa cctccaaaac
    26521 tttgcggtcg aactcgggca attcgtccaa aaataaaacg ccgtgatgcg ccaatgaaat
    26581 ctcgcccgga cgcggatccg agccgccgcc gaccatagcc gccgcgctgg cgctgtgatg
    26641 cggactgcgg aaaggacggt tgctgtcgag ttgttgttgg tggttgggca ggagcgaacg
    26701 caatgcccaa acttctacca attcgtcttc ggccagcggc ggcaggatgc cgggcagccg
    26761 ttgggagagc atagacttgc ctgttcccgg cgggcccatc atcaagaggc tgtgtccgcc
    26821 tgcggcggcg atttccaaag caaggcgggc agagtgctga cctttcacat cacacaaatc
    26881 aggttgtccg ccatgttcag acggcatttg cggtacggaa cattcggttt gcgccaaagg
    26941 ttcgataccg ttcaaatgag cggcgacttc gcccaaagag cgcgcgccgt aaacggtaat
    27001 gccgcgcatc acggcggctt gtcctgcgtt ttcttcaggc aaaacaaatg cacgttttgc
    27061 ctgcataccc tgccacgcca tcgccaacgc gccgcgcacg gggcgcaaca accccgacag
    27121 tgccaattcc cccgcaaact cgtattccgc cagcttttcg ggtgcaacct gccccgatgc
    27181 ggcaaggatg ccgattgcaa tcggcaaatc gaaacgcccc gactctttgg gcaggtcggc
    27241 aggggcgagg ttgacggtga tttttttggc ggggaattca aaaccgcttt gaataatggc
    27301 ggcacggacg cggtcgcggc tttcctttac ttccgtatcg ggcagtccga cgatgttgaa
    27361 atgcggcagg ccgttggcaa ggtgggcttc cacttcgacc aacggcgcat tcataccgct
    27421 caaggcgcgg ctgtaaacca aggcaagcga catatttcag acggccttat tcgccggctt
    27481 cggtttgctg cctgatttcg gcgacggctt cttcggcagc ggcttcagcc gcttccaatg
    27541 ctgcccgttc gggattttgc gcggcttcga gtttcgccag acgtgcttcc aaagccgcca
    27601 gtttggtacg ggttttaatc aaaacctgct gctggatgtc gaattcttcg cgcgtaacca
    27661 aatccatacg gttgaacgcg ccgcccagca tcgccttaat atttttttcc acatctttgg
    27721 cagggctgtt ggcgatggtt tcgctgattt tcgagccgac ttcttcaaaa agctgcttgc
    27781 cgaacataat ctgcatcctt cctgaacata tcaaattcaa gcggctattg tataaggaaa
    27841 aatgccgtct gaaaacgggc ggcggataat cgtcaaaaca taccgcgccc cctttgcggt
    27901 tgctaaaatt acacttcgag acacctgccg cctgaaaacg gatttcatat tccgcgcaac
    27961 gcccgattac attacaagac actacaaaac aatatgctgt tttaaatgat ttttccgatg
    28021 cgcatcgttt caaactcggc tttttaagcc attaagtgct ttgcaaacaa cagaaattgg
    28081 gttatgctga aacggattat ttacaatttc atatagtttc attacatatc ttattgtaat
    28141 tgaagataat ttatccgaat cccctttcgg gtatccggat tttccgttgt actttcatta
    28201 gaaaaacatt tcaggcgcaa gttgcttgca atttcaaagc ctgtaattct gaagttttag
    28261 acgaggaata gcacatgaat cccatgtaca tcacttttgc aatctatttg gttgcagtcc
    28321 ttctcatcgg gcttgccgct tatttttcca cgcgcaattt cgatgattat attttgggcg
    28381 ggcgcagcct gggcccgttt gttacggcga tgtcggcagg cgcgtccgat atgtccggct
    28441 ggcttttgat gggtctgccg ggcgcgattt atttgagcgg tttgaatgag gcttggattg
    28501 ccatcggcct cttggtcggc gcgtatttca actggctctt ggtggcgggc cgcctgcgcg
    28561 tgcataccga atatgccaac aatgcgctga cgctgccgga ttatttcttc caccgcttcg
    28621 gcgcgggcgg acacttgatg aaagtggttt ccgcactgat tatcctgttt ttcttcacga
    28681 tttattgcgc ctcgggcatt gtggcgggcg caaccctgtt ccaaagcctg tttgaaggta
    28741 tgacttacaa tcaggcaatg tggctgggcg cgggcgcgac catcgcctat accttcttgg
    28801 gcggcttctt ggcggtcagc tggacggata cgctgcaggc ttctttgatg attttcgccc
    28861 tgattttaac gccggtgatg gtctatctgg gcttgggcgg cgcggaacag atgtctgccg
    28921 cgattcaaag cgtcgccgca ggcacgggca aagaatacgg cagcctgttt gccggtacga
    28981 ccgtcatcgg catcatttcc accgccgcct ggggcttggg ctatttcgga cagccgcaca
    29041 ttttggcgcg ctttatggcc gccgaaagcg cgaaatcgct ggtatccgca cgccgcatcg
    29101 gcatgacttg gatggcgttg tgccttgcgg gcgcggtagc ggtcggttat ttcggcattg
    29161 cgtatttcgg tgcaaacccc gacaaagttt cttctatgag cggcaaccac gaacgcatct
    29221 tcatcgcgct ttccaccctg ctgttcaacc cttggatcgc cggcatcatt ttgagcgcga
    29281 ttttggcggc ggtgatgtcc accctgtcct gccagctttt ggtttgctcc agcgcgatta
    29341 ccgaagactt ctacaaaggc tttttgcgta aaaacgcgca acagtcggaa ttggtatggg
    29401 tcggccgcct gatggtattg gcgattgccg tgatttccat cctgattgct tccgatccga
    29461 acagcaaagt attgggactg gtgtcttacg cgtgggcagg tttcggtgcg gcattcggcc
    29521 cgattgtgat tctgtccgta ttgtggaaac gcatcactgc ctacggcgcg ttgtcaggca
    29581 tggttgcagg tgcgtcaacc gttgttgtgt gggcggaatg ggtaaaaaaa ccggctcgag
    29641 cggcaggcga aagcggcttg ttgacgatgt acgaaatcgt cccgggcttt attgtttgct
    29701 tgatcgttgc cgtgttggtt tccctgttca acaaagaacc ttcgcgcgaa atccaagaac
    29761 gctttgaaaa agccgacgca gattaccgcg ctgcccgata aacccctttc agacggcacg
    29821 gcatcccggt tcgtgccgtc tgaacccgct tccgggccaa acacaaataa accttgcctg
    29881 catacaggcc ttatttgtgt tttacccgtt tcattttcaa agaaacggaa gcaccgattc
    29941 tgcccgcatt tgaagccttg tatttcatct gcggatttat tacgatatta ccgtattcag
    30001 gccgcaccga tgccgtctga accccccgaa aaaactttgg aggatccaaa aaatgtttca
    30061 ttttgcattt ccggcacaaa ctgccctgcg ccaagcgata accgatgcct accgccgtaa
    30121 tgaaatcgaa gccgtacagg atatgttgca acgtgcacag atgagtgacg aagagcgcaa
    30181 cgccgcctcc gagcttgccc gccgtttggt tacccaagtc cgcgccggcc gcaccaaagc
    30241 cggcggcgtg gatgcgctga tgcacgagtt ttcactctcc agcgaagaag gcatcgcgct
    30301 gatgtgtctg gcagaagccc tgctgcgtat ccccgacaac gccacgcgcg accgcctgat
    30361 tgccgacaag atttcagacg gcaactggaa aagccatttg aacaacagcc cttccctctt
    30421 cgtcaatgct gccgcctggg gtctgctgat taccggcaag ctgaccgcca caaacgacaa
    30481 acaaatgagt tccgcactcg gccgcctgat cggcaaaggc ggcgcaccgc tcatccgcca
    30541 aggcgtaaat tacgccatgc ggcttctggg caaacagttc gtcaccggac agaccattga
    30601 agaagccctg caaaacggca aagaacgcga aaaaatgggc taccgcttct ccttcgatat
    30661 gttgggcgaa gccgcctaca cccaagccga tgccgaccgc tactaccgcg actatgtcaa
    30721 agccatccac gccatcggca aagatgcggc aggacaaggc gtttacgaag gcaacggcat
    30781 ttccgtcaaa ctctccgcca tccatccgcg ctactcgcgc gcccaacacg gccgcgtgat
    30841 gggcgaactg ttgcctcgcc tgaaagaatt gttccttttg ggtaaaaaat acgatatcgg
    30901 tatcaacatc gatgccgaag aagccaaccg tctggagctg tctttggatt tgatggaggc
    30961 actcgtttcc gaccccgatt tggccggcta caaaggcatc ggtttcgttg ttcaagccta
    31021 ccaaaaacgc tgcccgttcg tcatcgacta tctgattgac ctggcccgcc gcaacaacca
    31081 aaaactgatg atccgcctcg tcaaaggcgc gtattgggac agcgaaatca aatgggcgca
    31141 agtggacggc ttgaacggct atccgaccta cacccgcaaa gtgcataccg acatctccta
    31201 cctcgcctgc gcgcgcaaac tgctttccgc gcaagacgcg gtattcccgc aattcgccac
    31261 ccacaacgcc tacaccttgg gcgcaatcta ccaaatgggc aaaggcaaag attttgaaca
    31321 ccaatgcctg cacggtatgg gcgaaacctt gtacgaccaa gtcgtcggtc cgcaaaactt
    31381 aggccgccgc gtgcgcgtgt acgccccggt cggcacacac gaaaccctgc tcgcctactt
    31441 ggtgcgccgc ctgttggaaa acggcgcgaa ctcgtctttc gtcaaccaaa tcgttgatga
    31501 aaacatcagc atcgaccgcc tgattaagag cccgttcgac accatcgccg aacaaggcat
    31561 ccacctgcac aacgccctgc cgctgccgcg cgatttgtac ggcaaatgcc gtctgaactc
    31621 gcaaggcgtg gacttgagca acgaaaacgt attgcagcag cttcaagaac agatgaacaa
    31681 agccgccgca caagacttcc acgccgcatc catcgtcaac ggcaaagccc gcgatgtcgg
    31741 cgaagcgcaa ccgattaaaa accctgccga ccacggcgat gttgtcggta cggtcagctt
    31801 tgccgatgcc gcgcttgccc aagaagcggt tggcgcagcc gttgccgcgt tccccgaatg
    31861 gagtgcgaca cctgccgccg aacgcgccgc ctgcctgcgc cgtttcgccg acctgctcga
    31921 acagcacacc cccgcgctga tgatgcttgc cgtgcgcgaa gcgggcaaaa cgctgaacaa
    31981 cgccattgcc gaagtgcgcg aagccgtcga tttctgccgc tactacgcaa acgaagccga
    32041 acacaccctg cctcaagacg caaaagccgt cggcgcgatt gtcgccatca gcccgtggaa
    32101 cttcccgctc gccatcttta ccggcgaagt cgtgtccgcc ctggcggcag gcaacaccgt
    32161 catcgccaaa cctgccgaac aaaccagcct gattgccggt tatgccgttt ccctcatgca
    32221 cgaagccggc atcccgactt ccgccctgca actcatcctc ggcgcaggcg acacgggtgc
    32281 ggcattgacc aacgatgcca gcatcggcgg cgtgattttc acaggctcga ccgaagtggc
    32341 gcgcctgatc aacaaagccc ttgccaaacg cggcgacaat cccgtcctga ttgccgaaac
    32401 cggcggacaa aacgccatga ttgtcgattc caccgcactt gccgaacaag tctgcgccga
    32461 cgtattgaac tccgccttcg acagcgcggg acaacgctgc tccgccctgc gtatcttatg
    32521 cgtccaagaa gacgttgccg accgtatgct cgacatgatc aaaggcgcga tggacgaact
    32581 cgtcgtcggc aaaccgattc agctcactac cgatgtcggc cccgtcatcg atgccgaagc
    32641 gcagcaaaac ctgttgaacc acatcaacaa aatgaaaggc gttgccaagt cttaccacga
    32701 agtcaaagcc gccgccgatg tcgattccga aaaatccacg ttcgttcgcc ccatcctgtt
    32761 tgaattgaac aacctcaacg aactgcaacg cgaagtcttc ggccccgtcc tgcacgtcgt
    32821 ccgctaccgc gccgacgaac tcgacagcgt catcgaccaa atcaacagca aaggctacgc
    32881 cctgacccac ggcgtacaca gccgcatcga aggcacggta cgccacatcc gcagccgcat
    32941 cgaagccggc aacgtttacg tcaaccgcaa catcgtcggt gcagtcgtcg gcgtacagcc
    33001 cttcggcgga cacggtctgt ccggcacagg ccccaaagca ggaggttcgt tctacctgca
    33061 aaaactgacc cgcatccccg aatgggttgc cccgaccctg agccaaatcg gacaggcaga
    33121 cgaagccgcg ctcaaacgcc tcgaagccct gattcacaaa ctgccgttca acgccgaaga
    33181 gaaaaaagcc gcagcggccg ctttgggaca cgcccgcatc cgcaccctgc gccgtgccga
    33241 aaccgtcctt accggcccga ccggcgagcg caacagcatc tcatggcacg cgccaaaacg
    33301 cgtttggata cacggcggca gcacggttca agcctttgcc gcactgaccg gacttgccgc
    33361 ctccggcgta caggcagtgg tcgaacccga cagcccgctg gcttcctaca ccgccgactt
    33421 ggacggtttg ctgcaaatca gcggcaaacc cgaaaacacc ggtgtgaacc acgtcgccgc
    33481 cctaagccca ctggacagca gccgcaagca ggaactggca aaccgcgacg gcgcactcat
    33541 ccgcatcctc ccgtccggac aaggcttgga catcctgcaa gtgtttgaag aaatttcttg
    33601 cagcgtcaac accacagccg ccggcggcaa cgccggcctg atggcggttg ccgactgatt
    33661 ttgccaaacc gcccgggcgc ggcccgtgaa caaatgccgt ctgaaaacct ttcaggcggc
    33721 attttatagt ggattaacaa aaatcaggac aaggcggcgg gccgcagaca gtacaaatag
    33781 tacggcaagg cgggccaacg ctgtactggt ttaaattcaa ttcactatat gtgttggcac
    33841 atcgctccaa acctgatata atccgccttt caacatcagt gaaaatcgtt ctttttagtc
    33901 agttaacatt aaatttcgga gtcgaaaaat gaatccagcc ccaaaaa
//
LOCUS       DAWTHD010000008        33485 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWTHD010000008 DAWTHD010000000
VERSION     DAWTHD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33485
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.008"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(84..575)
                     /locus_tag="WM704_000287"
     CDS             complement(84..575)
                     /locus_tag="WM704_000287"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HFA9339798.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(649..1407)
                     /locus_tag="WM704_000288"
     CDS             complement(649..1407)
                     /locus_tag="WM704_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9339799.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(1477..2343)
                     /locus_tag="WM704_000289"
     CDS             complement(1477..2343)
                     /locus_tag="WM704_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9339800.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(2392..2670)
                     /locus_tag="WM704_000290"
     CDS             complement(2392..2670)
                     /locus_tag="WM704_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HFA9339801.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(2670..2960)
                     /locus_tag="WM704_000291"
     CDS             complement(2670..2960)
                     /locus_tag="WM704_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFA9339802.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(2963..3493)
                     /locus_tag="WM704_000292"
     CDS             complement(2963..3493)
                     /locus_tag="WM704_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFA9339803.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            3801..4826
                     /locus_tag="WM704_000293"
     CDS             3801..4826
                     /locus_tag="WM704_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9339804.1"
                     /translation="MKLIAASIAVAASFQAHARLGGLNIRSNLDEPFSGSITVTGEEA
                     KALLGGGSVTVSEKGLTAKVHKLDDKAVIAVSSAQAVRDPVLVFRIGAGAQVREYTAI
                     LDPVGCSPKTKSALSDGKTHRKTAPKAESQENQNAKALRKTDKKDSANSAVKPAHNGK
                     THTVRKGETLKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRI
                     KAAAPKKIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPIEKPVEKPVEKPEAKVAA
                     PEAKAEKPAVRPEPKPAVSETPASATERQPGPVPAANTAASETAAESAPPRSRRFCHR
                     HADRRNR"
     gene            4963..6117
                     /locus_tag="WM704_000294"
     CDS             4963..6117
                     /locus_tag="WM704_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HFA9339805.1"
                     /translation="MLLAGGGTALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAAD
                     DGIKITFAEVETPATPEPAPKNDVNDTLALGGESEEELSAKQTFDVETDTPSNRIDLD
                     FDSLAAAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTP
                     EPESVAQTAENKPETVDTDFYNNLFSNNHIGTEETASAKPAAPSGLAGFLKASSPETI
                     LEKTVAEVQTPEELHDFLKVYETGAVAETAPETPDFNAAADDLSALLQPAEAPAVEEN
                     AAEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSD
                     APSEAKYDLAEMHLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(6290..6706)
                     /gene="gloA"
                     /locus_tag="WM704_000295"
     CDS             complement(6290..6706)
                     /gene="gloA"
                     /locus_tag="WM704_000295"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFA9339806.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(6731..7900)
                     /gene="lapB"
                     /locus_tag="WM704_000296"
     CDS             complement(6731..7900)
                     /gene="lapB"
                     /locus_tag="WM704_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFA9339807.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(7911..8225)
                     /locus_tag="WM704_000297"
     CDS             complement(7911..8225)
                     /locus_tag="WM704_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HFA9339808.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            8535..9533
                     /gene="ilvE"
                     /locus_tag="WM704_000298"
     CDS             8535..9533
                     /gene="ilvE"
                     /locus_tag="WM704_000298"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFA9339809.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(9778..10563)
                     /gene="fabI"
                     /locus_tag="WM704_000299"
     CDS             complement(9778..10563)
                     /gene="fabI"
                     /locus_tag="WM704_000299"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFA9339810.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(10718..11539)
                     /gene="dapD"
                     /locus_tag="WM704_000300"
     CDS             complement(10718..11539)
                     /gene="dapD"
                     /locus_tag="WM704_000300"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFA9339811.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(11749..13392)
                     /gene="pgi"
                     /locus_tag="WM704_000301"
     CDS             complement(11749..13392)
                     /gene="pgi"
                     /locus_tag="WM704_000301"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFA9339812.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            13902..15134
                     /gene="pilG"
                     /locus_tag="WM704_000302"
     CDS             13902..15134
                     /gene="pilG"
                     /locus_tag="WM704_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFA9339813.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            15208..16068
                     /locus_tag="WM704_000303"
     CDS             15208..16068
                     /locus_tag="WM704_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFA9339814.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            16065..16697
                     /locus_tag="WM704_000304"
     CDS             16065..16697
                     /locus_tag="WM704_000304"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFA9339815.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            16690..16899
                     /locus_tag="WM704_000305"
     CDS             16690..16899
                     /locus_tag="WM704_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFA9339816.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(16985..18661)
                     /gene="pilB"
                     /locus_tag="WM704_000306"
     CDS             complement(16985..18661)
                     /gene="pilB"
                     /locus_tag="WM704_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFA9339817.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(18907..18978)
                     /locus_tag="WM704_000307"
     tRNA            complement(18907..18978)
                     /locus_tag="WM704_000307"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18943..18945),aa:Arg,seq:cct)
     gene            complement(18999..19445)
                     /locus_tag="WM704_000308"
     CDS             complement(18999..19445)
                     /locus_tag="WM704_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFA9339818.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(19449..20423)
                     /locus_tag="WM704_000309"
     CDS             complement(19449..20423)
                     /locus_tag="WM704_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFA9339819.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            complement(20439..20627)
                     /locus_tag="WM704_000310"
     CDS             complement(20439..20627)
                     /locus_tag="WM704_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339820.1"
                     /translation="MVNIRTPLCEIGHCIVICLFCQSLRGSGNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            20647..20955
                     /gene="rplU"
                     /locus_tag="WM704_000311"
     CDS             20647..20955
                     /gene="rplU"
                     /locus_tag="WM704_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFA9339821.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            20980..21252
                     /gene="rpmA"
                     /locus_tag="WM704_000312"
     CDS             20980..21252
                     /gene="rpmA"
                     /locus_tag="WM704_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFA9339822.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            21487..22671
                     /gene="ubiM"
                     /locus_tag="WM704_000313"
     CDS             21487..22671
                     /gene="ubiM"
                     /locus_tag="WM704_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFA9339823.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEIVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(22860..23015)
                     /gene="rpmG"
                     /locus_tag="WM704_000314"
     CDS             complement(22860..23015)
                     /gene="rpmG"
                     /locus_tag="WM704_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFA9339824.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(23047..23280)
                     /gene="rpmB"
                     /locus_tag="WM704_000315"
     CDS             complement(23047..23280)
                     /gene="rpmB"
                     /locus_tag="WM704_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFA9339825.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(23318..23467)
                     /locus_tag="WM704_000316"
     CDS             complement(23318..23467)
                     /locus_tag="WM704_000316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339826.1"
                     /translation="MQKLPKSAIIAPHLFRTCKRSADAALGRLTRAKHLFFFKTLETA
                     LFFVV"
     gene            complement(23530..25053)
                     /gene="farB"
                     /locus_tag="WM704_000317"
     CDS             complement(23530..25053)
                     /gene="farB"
                     /locus_tag="WM704_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFA9339827.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(25077..26231)
                     /gene="farA"
                     /locus_tag="WM704_000318"
     CDS             complement(25077..26231)
                     /gene="farA"
                     /locus_tag="WM704_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFA9339828.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      26472..26516
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            26520..26993
                     /gene="queF"
                     /locus_tag="WM704_000319"
     CDS             26520..26993
                     /gene="queF"
                     /locus_tag="WM704_000319"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFA9339829.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            27068..27754
                     /locus_tag="WM704_000320"
     CDS             27068..27754
                     /locus_tag="WM704_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFA9339830.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(28104..29396)
                     /locus_tag="WM704_000321"
     CDS             complement(28104..29396)
                     /locus_tag="WM704_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFA9339831.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGDTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHS
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            29473..30441
                     /locus_tag="WM704_000322"
     CDS             29473..30441
                     /locus_tag="WM704_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339832.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(30642..32111)
                     /locus_tag="WM704_000323"
     CDS             complement(30642..32111)
                     /locus_tag="WM704_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9339833.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGFSITREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRAYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(32353..33450)
                     /locus_tag="WM704_000324"
                     /pseudo
     CDS             complement(32353..33450)
                     /locus_tag="WM704_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         7964 a         9626 c         8326 g         7569 t
ORIGIN      
        1 ataggggcga cggaaaggcg gcgcggggga agggtgtgtg tattgtcgtt cattgtcgat
       61 atttcatcat tgcggactca atttcaatcc ggaacaacct tcatttttgg ggaaatccgc
      121 gtcatgaaag agtccagcag tatggttacc ggcccgtcgt tgcagagcga cacctgcata
      181 tgcgtgcgga aacgccctgt ttcgacatga atcccgtgtc cgcgtaacag ctccgccgtt
      241 cgcaggtaaa gccgttgcgc ctgttctgcc ggcgcggctt gggaaaacga aggccgccgc
      301 ccgcttgccg cgtcggcata aagcgtaaac tgcgacacca gcagcaccgc gccgccgaca
      361 tctttcaaag acaggttcag cttgcccgct tcgtcttcaa acacgcgcaa atgtgcgatt
      421 ttgtcggcga tatagcgtgc atctttttct gtgtcgctat gcgttacacc gagtaacacg
      481 acaaacccgc cgtcgatttt gccgcaggtt tccgtgccgg cttcggacac gacatccacc
      541 tttgcaccta ccgttttctg tatgaccgca cgcataagcc gtcctcctta cctgaacgtg
      601 caatgccgtc tgaaaaggct tcagacagca tcggtatcgg aaatgccatt acggtttgac
      661 accgttttct tccaaaaggg catcgatttt caagcgggct ttttcctgcc tcaaaccttg
      721 ttccaactgg tttctgacca actcgaaagg ctgcgcgtcg gggtttttcc cgaccgcgcc
      781 gagtttgaac aggtaatagc gttcgcccaa tttgaccgga ttgcgggtaa cgtcgccacg
      841 gttcataccg gcaaactgcg aagccagcgg ctcgggaagc tgctgcgcca taatgaaacc
      901 gtcgaacgcc tgctcgtcgt tcggataacg cttcatcagc ccttcaaaag acagcccttt
      961 gagcaggagc tgctgcgcct gacgcgcctc ctcttcggtt gcgaagctga cctgctgcaa
     1021 tttgatcatg cggatttgcc gctcataaaa ctgacgcagt gcgctttcgg aaaccgtttc
     1081 cgaacgttcc agaaaacgga cgtactcctc ggcataaaaa gacgcttcgg cgattttaaa
     1141 gcggttttgg acatccttat ccttatccaa accttccttc aatgccctgt ttttcaaaac
     1201 ttccaaagtt tgcagccggc ggacggcatc gtttcggatt gcctgcccgt ccggtctttg
     1261 ggactgctcc gcatgccggt ctgcctgctg catgatctgc gccaccagcg tatccaccaa
     1321 agccgggtcg atttcgggcg ctttggctgc cgcaaaacct gccaacattg cagcaataac
     1381 tgcggcagcg gtctttttct gtttcatggt attccctgtc ctgcgcggcg gcaaaacgcc
     1441 tgtatgaacg tcttgccgcc gtatcccgtt ttcagattat tttgcaggtt tgatgtttgc
     1501 cttacccaac agcgcaccga cggcacggtc aatccgttcc gcctgaaggt tgccggcaat
     1561 ctgtcctttc atttcgtcaa aagaaggcac tttcacctcg cggctgtcgt tgacataata
     1621 aacgccgtag aaatcgccgt ttttcagcgg cgttgccgta aattcgcctt ttttcaagtc
     1681 cttaattgcc tgataaagcg gcggaacacc ctgttccaaa tctttcagcg gcacatatcc
     1741 gtccggcgcg ccggtccgtt tggtgcggtc gttgagcgag tattgtttca aaacggcatc
     1801 aaaacctttt ttcgccttca aatcggcaac cgcttttttc gcattttctt ccttgtcggt
     1861 caggatttcg cccaactgga cttcctgcgt gcctttataa aaaccgctga tattgtcgta
     1921 aacggctttt acttcctgct cggaaaccgg ttgggttttg gcgatatgca gtgcgtatgc
     1981 ctcgccgttc aagccatatt ttaccgcctg ccaaacggtt ttgaaggacg gtttcttgtc
     2041 gtcgcccgac tttttcgctt cggcacgcaa tttggcaagc gcatctttaa actctgccga
     2101 ccggtcgagt ttcaggcgtt tcacttcctg tgcgaccacg gtgttgacca cttcgttttc
     2161 cagcagggat tggcgcagtt gcggcgtgtc ttcggcacgg ctgttttccg cacggaatgc
     2221 ggcaacctgc gcatcgatga cggaactgtc gattttctga ccgttaacgg ttgccagcgt
     2281 ttgggcaaac aggctgccgg aacaggcaag cagcgcaacg gaagtcagga tttttgcttt
     2341 cataatcttc tctctttaaa ttttaaattg aaatattcag gcagacttca ttcagtccgc
     2401 cgtatggaaa tactcgtcag gggtagccgc ccggatgccg agcgcgtgaa tcatgccgcc
     2461 tgaaaataaa tctttgagca gcgatttgac cgttttctga cggttcagac ggcttaaacc
     2521 ttcaaaacgg ccgctgacga ccaaaacggc ataatgtccg ccgccggtat tgcccgcgtg
     2581 tcctttgtgc agatggctct catcgccgat ttccaacacc agaggatcga gcgtctgcag
     2641 gcgttcgcgg atcaaatcga cagccggcat tatttgaaca ccgctttaaa cggcttgatc
     2701 agcacttcgc tgtacacgcc ggcatgaaca tagggatcgt cttcagccca agcctgcgcc
     2761 gcatccaaag actcgaactg tgccacaatc aagctgcccg aaacacgttc gggattgtcc
     2821 ggcagcaggt ttgggcctgc cgtcagcaga cggccttccg atttcagcgt ttccagccgt
     2881 ttaaagtgtt cgggacgtgc cgccatacgc gcttcatgca catcctcccc gtctgttgcc
     2941 agcaacataa aatattccac agtcaatcct cctttttcag ataggtactc agataaatac
     3001 cctgaataat aaagaaaaaa agcatcagcg cagtcgaacc gaacatctta tagttaaccc
     3061 attgcgcttc aaacctagta aacacaaacc agttggcaat acccataaaa atcagaaaac
     3121 cgacccacat atatgtcaat tttccccata cggcatccgg aagctgaatc tccctgccga
     3181 tactcgcttt caagccgttt ttacccgcaa ggtggctgcc cagcaggaat aacgccccgc
     3241 accagaacaa tactgtcggc ttccacataa tgaagcggct gtcgcccaaa acaatggttg
     3301 cgccgccgaa tacgacaatc agcaccagtc cgacccactg catcgtatcc agccttttat
     3361 gcttccaata caggaaagcc gcctgaacca cgcctgcaac caaggcaacc gccgcagcgg
     3421 caatcatatt tttggtaacg gtataagtag caaaaaacaa gatgacggac aaaaggtcgc
     3481 tgacaaattt catatccaaa tgagaaagca aatcaaataa acgctaatca tacaggcaga
     3541 accctatctt tgcacctgca aaaccaacaa attttgtttt gattaatgca aatatgcaca
     3601 gatgtttttg aaaaaagatg gaaatatgtc ataatttata aaattggctt taaaatacag
     3661 cttcggtcgg aacctctgcc tatccgcacc ctatcgtccg tattgcctat gcctgatgac
     3721 gaaatttaca gactgtacgc ggtcaaaccg tattcagccg ccaacccaca ggggatacat
     3781 cttgcaaaaa caacagacaa atcaaactga ttgccgcctc catcgcagtt gccgcatcct
     3841 ttcaggcaca tgcgcgactg ggcggactga atatccggtc caaccttgac gaaccctttt
     3901 ccggcagcat taccgtaacc ggcgaagaag ccaaagccct gctaggcggc ggcagcgtta
     3961 ccgtttccga aaaaggcctg accgccaaag tccacaagtt ggacgataaa gccgtcattg
     4021 ccgtttcttc cgcacaggca gtccgcgatc ccgtcttggt gttccgcatc ggtgcaggcg
     4081 cgcaggtacg cgaatacacc gccatcctcg atcctgtcgg ctgctcgccc aaaaccaaat
     4141 ctgcactttc agacggcaag acacaccgca aaaccgctcc gaaagcagag tcccaagaaa
     4201 atcaaaacgc caaagccctc cgcaaaaccg ataaaaaaga cagcgcaaac tcagccgtca
     4261 aaccggcgca taacggcaaa acccataccg tccgcaaagg cgaaacgctc aaacagattg
     4321 ccgccgccat ccgtccgaaa cacctgacgc tcgaacaggt tgccgatgcg ctgctgaagg
     4381 caaacccaaa tgtttccgca cacggcagac tgcgtgcggg cagcgtgctt cacattccga
     4441 atctgaacag gatcaaagcg gcagcaccca aaaaaatcaa agcggaacaa cccaaaccgc
     4501 aaacggcgaa acccaaagcc gaaactgcat ccatgccgtc tgaaccgtcc aaacaggcaa
     4561 cggtagagaa accgatagag aaacctgtcg aaaaaccggt tgaaaaacct gaagcaaaag
     4621 ttgccgcgcc cgaagcaaaa gcggaaaaac cggccgttcg acccgaaccc aaacccgcag
     4681 tgtccgaaac tccggcttcg gcgacagaac gccaacccgg acctgtaccc gctgcaaata
     4741 ctgccgcatc ggaaaccgct gccgaatccg cccccccaag aagccgccgc ttctgccatc
     4801 gacacgccga ccgacgaaac cggtaacacc gtttccgaac ctgttgcgga aacagccagt
     4861 gaagttgccg ccgaacccgt tgccgaacct gtcgaacagg tttctgccaa agaagaaacc
     4921 gaaagcggac tgtttgacgg tctgttcggc ggttcgtaca ccttgctgct tgccggcgga
     4981 ggcacggcat tgatcgccct gctgctgctt ttacgccttg cccaatccaa acgcgcgcgc
     5041 cgtaccgaag aatccgtccc tgaagaagag cctgaccttg acgacgcggc agacgacggc
     5101 ataaaaatca cctttgccga agtcgaaact ccggcaacgc ccgaacccgc tccgaaaaac
     5161 gatgtaaacg acacacttgc cttaggtggg gaatctgaag aagagttatc ggcaaaacaa
     5221 acgttcgatg tcgaaaccga tacgccttcc aaccgcatcg acttggattt cgacagcctg
     5281 gcagccgcgc aaaacggcat tttgtccggc gcacttacgc aggatgaaga aacccaaaaa
     5341 cgcgcggatg ccgattggaa cgccatcgaa tccacagaca gcgtgtacga gcctgagacc
     5401 ttcaacccgt acaaccctgt cgaaatcgtc atcgacacgc ccgaaccgga atctgtcgcc
     5461 caaactgccg aaaacaaacc ggaaaccgtc gataccgatt tctacaacaa cctgttctca
     5521 aacaaccata tcggcacaga agaaacagct tccgcaaaac ctgccgcacc ctccggactg
     5581 gcaggcttcc tgaaggcttc ctcgcccgaa accatcttgg aaaaaacagt tgccgaagtc
     5641 caaacaccgg aagagttgca cgatttcctg aaagtgtacg aaaccggtgc cgtcgcggaa
     5701 actgcgcctg aaacgcccga tttcaacgcc gccgcagacg atttgtccgc attgcttcaa
     5761 cctgccgaag caccggccgt tgaggaaaat gcagcggaaa tcactttgga aacgcctgat
     5821 tccaacacct ctgaggcaga cgctttgccc gacttcctga aagacggcga ggaggaaacg
     5881 gtagattgga gcatctacct ctcggaagaa aatatcccaa ataatgcaga taccggtttc
     5941 ccttcggaat ctgtaggttc tgacgcgcct tccgaagcga aatacgacct tgccgaaatg
     6001 catctcgaaa tcggcgaccg cgatgccgct gccgagacag tgcagaaatt gctggaagaa
     6061 gcggaaggcg acgtactcaa acgtgcccaa gcattggcgc aggaattggg tatttgattc
     6121 ccaactgccc tttcgcagat caaggatgcc gtttcagacg gcatcttttt ttgccttatc
     6181 ggtgtaacgg ataaagtttg aaccggcaca ggctcaaaca gcaggccgac ggcaacaaaa
     6241 tgccgtccga aacccctaaa ggcttcagac ggcattggcg gcggcggtat caggtattcg
     6301 cataggcaac cgaatcgtcg ccgcttttct tttgaacgaa ctcgattttg catccgtcgg
     6361 ggtcttctac gaaggctatc acggttgtgc cgtgtttcat caggccggct tcgcggacga
     6421 cgtttccgcc ctgccgcttc acgcgttcgc aggcttcgta ggcatcgtcc acttcaaccg
     6481 cgatgtgtcc gtaggcgtcg cccaagtcgt atcgttccgt atcccagttg tgcgtcagtt
     6541 ccaaaaccgt gctgtcggtt tcgtcgccgt aaccgacgaa ggcaagggta aatctgcctt
     6601 cgggatagtc ttttcggcgg agcagtttca tacccaaaac gttttggtag gaatcgaggg
     6661 atttttcgag attgcccacg cggagcatag tatggagtaa gcgcattttt cgtgttcctc
     6721 tcggtggtgg ttaaacttcg attttattcg gcgtaaacgt ctgccatttg ttgcaggcgg
     6781 gacagtgcca gaaaaagact tgggatttga agtggcagtt gcggcaacgg tacatcacgc
     6841 tgcgctggag ctgccgtccg ataaccgaac gcatcatgtc ggcatcggct ttccaagccg
     6901 gatccaaatc gctgagtttc aaaccgagca ggcggtacac gccgttaagg tcgggcttgc
     6961 ggcggacaag ctcgacggcg gtttgcgcgg cttctttctc gcccttaagc agcagggatt
     7021 tctcgtacac gacattgatc aggtcaagtt cgggaaacgt ctgcatatat cctgtcagac
     7081 ggttcaagcc ttcttcaggt tttccctgcg cggcataggc ttcgtaaagc ttctcgccga
     7141 ccatgctcaa gtatgcatgg ttttgctgct cgatggcggc ataggcttcg acggcggcag
     7201 ggaaattgcc ttgtcggtgt tcaatgtcgc ccaaaatcat gttggcgcgg gtgcattttt
     7261 tgttggcttc gagtgccttg ccgacattga aacgcgcggc atcgaaattg gacttgaaca
     7321 gcgcggcttg ggcaagttcg caataaaact gtgcaatctc aaactgatat gtctgttcgt
     7381 cgtgactaag aagttgggcg gtttcaaccg ctttttccca atccctgtcc tgctggtaga
     7441 tattgagcag gtgctgtctg gcttcacgcg ccatttcacc gtcttgcagc cccaaaaaaa
     7501 tctgttcggc acgatcgacc aaacccgcgc tttggtagtt ttgcgccaat tcaaacagga
     7561 cgcgcgcgcg cttttcgccg accgtatcgg gagaatcgag cattgtccgg tgtatgttga
     7621 tggctttgtc gttttcgccg cgctgacggt aaagtttgcc gagggtaagg ttcaaatcat
     7681 acgattgcgg ccggccgtcg acgacttccg ccaactccct tgccgcgcgc ccgctgttgc
     7741 ggtcgaccaa agcgtccagg cttttataaa atcccgaagg gatgcttttt gcctgcttca
     7801 atacggtttt catatccacg cgggcggcaa accagcccat cgtgaagaag acgggcaaaa
     7861 ggataatcgg cagcaggata atccacaatt cgttgtccat atcggctttc ttaaggctgt
     7921 ttggcagatt cggcagcatt ttgtatcggc ggtgcagtca atttctgtcc gctcaagcgc
     7981 gcacttttct tcacttccgc acgcaggcgg ctgttttcgc cgcgcaagga cagcagccgc
     8041 ccgaacaggg caaacattcc gaacacgatg ccgacgacaa acgcgccgaa caatacgaca
     8101 atcagcggca gattgacact ctgccccgga agataggaaa aggtaacggc atccatatta
     8161 atgacggcaa gcagcaggaa gagcagcagg ataatgattt tgatgacggt atagataagt
     8221 ttcatgtttg ctccgtaaat aaaaacgccg cacggacagc ttcgcactct gaaccaagat
     8281 tgcgttagtt taaacgattt gcacggtttc ggatagaatg cggcagcgtg ttcggacggc
     8341 atacggagta tgcgattgcc gacaatttta ccaaatacac cgctcctttc catttgaaaa
     8401 ataacggatt ggacaccgca tcgacagaaa aacccgccgc gcacttgtca aaaccctgtt
     8461 tgcaggcgta tctttacaac cttcaaattc aaaccgttca ttgaaacata tcagaataag
     8521 aaaggcttta catcatgagc agacccgtac ccgccgtatt cggcagcgtt tttcacagtc
     8581 aaatgcccgt cctcgcctac cgcgaaggca aatggcagcc gaccgaatgg caatcttccc
     8641 aagaccttac ccttgcaccc ggcgcgcatg ccctgcatta cggcagcgaa tgctttgaag
     8701 gtttgaaggc cttccgtcag gcagacggta aaatcgtgct gttccgtccg accgccaata
     8761 tcgcgcgtat gcggcaaagt gcggacattt tgcacctgcc gcgccccgaa acccaagctt
     8821 atctcgatgc tttggtcgaa ctggtcaaac gtgccgccga cgaaattccc gatgcgcctg
     8881 ccgcgctgta cctgcgcccg accttaatcg gtaccgatcc cgttatcggc aaggccggtt
     8941 ctccttccga aaccgccctg ctgtatattt tggcttcccc cgtcggcgac tatttcaaag
     9001 tcggctcgcc cgtgaaaatc ttggtggaaa ccgaacacat ccgctgcgcc ccgcatatgg
     9061 gccgcgtcaa atgcggcggc aactacgctt ccgccatgca ctggttgctg aaggcgaaag
     9121 ccgaatacgg cgcaaatcaa gtcctgttct gcccgaacgg cgacgttcag gaaaccggcg
     9181 catccaactt tatcctgatt aacggcgatg aaatcattac caaaccgttg acagacgaat
     9241 tcctgcacgg cgttacccgc gattccgtgc tgactgtcgc caaagatttg ggctataccg
     9301 tcagcgaacg taatttcacg gttgacgaac tgaaagctgc tgtggaaaac ggtgcggaag
     9361 cgattttgac cggtacggcc gccgtcatct cgcccgttac ttccttcgtc atcggcggca
     9421 aagaaatcga agtgaaaaac caagaacgcg gctatgccat ccgtaaggcg attaccgaca
     9481 tccagtacgg tttggcggaa gacaaatacg gctggctggt cgaagtgtgc tgatgcttta
     9541 aataaaaaaa tgccgtctga aacccgtttg gcgtttcgga cggcattttc gcgttcggac
     9601 cgtttccgcc gcacctgcag caagtcggta caaaggcaat cggttaaaac aagcgtccgc
     9661 atttcccatc ccgcctgccg caagtcgggc atttccctgg aaatacgctt caaacggcaa
     9721 accgccgccc gaaaccgata ctcggcacgg acggcgcacg aatttgaaaa cggcggatta
     9781 tccctcggtg ctcaaggcat tgatgctgta accgccgtca acataagtga tttcgccggt
     9841 aatgccggac gacaggtcgg acagcaggaa ggcggcagta ttcccgactt cttcgatggt
     9901 aacgttgcgg cgcagcgggt tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc
     9961 gatgccggag gcggcgagcg ttttgatcgg gccggcggaa atgccgttgc agcggatgcc
    10021 ctctttaccc agacaggcgg cggtaaagcg gatgcctgcc tcaaggctgg cttttgccat
    10081 acccatcacg ttgtaattcg gaatcgcgcg taccgcgccc aagtagctaa gggcgacgat
    10141 ggcggagttt ctgccgcgca tcatcggacg ggcggctttt gccaatgcgg gcaggctgta
    10201 tgcggaaatt tcgtgtgcgg tgttgaacgc ttcgcggctg atgctgtcga ggaagtcgcc
    10261 gctcaaggct tctttcggcg cgaagccgat ggaatgcacc aaaccgtcca agccatccca
    10321 atgtttgcct aagtcggcga acacttggtt gatttcgtcg tcgctggcga catcgcagcg
    10381 gaatacgagt tcggaatcta attccgccgc cattttgcgg acgcgctctt ccagtttgtc
    10441 cacaacgtag gtaaacgcca gttccgcgcc ctgttcgcgg caggctttgg cgatgccgta
    10501 agcgatggaa cgctcggaaa tcatgccggt aatcagaatt tttttgcctt gcagaaaacc
    10561 cattttgtat ccttcaaata gtgttcggtt cgttctaaac cgttgattat agcaaattgt
    10621 ccctgtttct gtgttttcac gttgcagcgt gcggacggca atgccgtccg aagcggattt
    10681 cagacggcat tggacgtttc aaatacggtt taaggcatca gatgccgcgc aagagttcgt
    10741 tcacgctggt tttggcgcgg gtttgcgcgt ccacgcgttt gacgatgacg gcacagtaaa
    10801 ggctgtggct gccgtctttg gaaggcaggc tgccggacac gacgaccgaa cctgccggta
    10861 cgcggccttg gtagatttcg cctgtggtgc ggtcgaagat tttggtggat tgaccgatga
    10921 acacgcccat cgaaatcacg ctgccttctt cgacaatcgc gccttcgacg atttcggaac
    10981 gcgcgccgat gaagcagttg tcttcaatga tggtgggtgc ggcctgcagg ggctcgagta
    11041 cgccgccgat gccgacaccg ccgctcaagt ggacgttttt gccgatttgc gcgcaagagc
    11101 cgacggtcgc ccaagtatcg accatcgcgc cttcgtcgac gtatgcgccg atgttgacgt
    11161 aagacggcat cagcacggcg tttttggcaa caaagctgcc gcgtcgggca accgcacccg
    11221 gaactgcgcg gaagcctgcg tttttgaact cgtcttcaga ccagtcggca aatttggtcg
    11281 gcactttgtc gaagtatttg ttcacgccgt cgttgaggac ttcgttgtct tggatgcgga
    11341 aggacagcaa cacggctttt ttcgcccatt cgttgacttt ccactcgccc acgcccaagc
    11401 gttcggcgac gcgcagtttg cctgaatcga gttggcggat ggtttccaac acggcttctt
    11461 tgacttcggg ggcaacggtg gtcggagtga tgtccgcgcg gttttcaaag gcggtttcga
    11521 taatgttttg caaagacata atgtttcctt ggttcgagat atttcgggac gcttgccggt
    11581 attgttcaga cggcatggcg gttggaacaa acgtgctatt gtgccataca aacgcccgcc
    11641 cgtcagccgc gccgcaggct tgccggatgc cggcgtttcg gaatcgggcg ggcggcaggc
    11701 atatgtcttt ttccgcgccc gaatcaatac agaaaggcgg cggtactttt atgccgcgcc
    11761 gccgcctttc agacggcatt cgcggtaaaa cgccatcagc ccttcggtcg aggcatcgtg
    11821 tacggacgtg ccgccttcca attcgccgat gatggttttt gccaactgtt tgccgtactc
    11881 aaccccccac tgatcgaagg ggttgacgtt ccatatcgcg ccttggacga aggttttgtg
    11941 ttcgtaagcc gccatcagca tacccagatt gcagggcgtg aggcggtcga gcaggatgct
    12001 gttgctgggg cggttgccgg ggaactcttt gtgcggcgcg aggcgttcgc gttccgcttc
    12061 gggcaaatct gccagttcgg cgcgtgcttc gtctaaggtt ttacccttca tcagggcttc
    12121 cgcttgggca aaggcgttgg caacggtaaa acggctgcgt ccgtcctccg cgccctgcgc
    12181 cgtcatcggg acgataaagt cgcagggaat caggcgcgtg ccttggtgga gcagttggaa
    12241 ataggcgtgc tggcagttga ccccttcacc gccgaacacg atgccgcccg ttttgcacgc
    12301 ggcgggactg ccgtctgaag cgcggctttt gcccaaactc tccatatcga gctggttcag
    12361 ccacgccggc agcaggcgca ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg
    12421 ctggaaattg ttataccaca cggcaatcag tgccatcaga acgggaatgt tgcgacgcgg
    12481 gggcgtgtgg aaaaaatggc tgtccatcgc gtgcgccccc gctagcagtt cgcggaaacg
    12541 cgccccgcca accgcaacca tcacgggcaa accgacgggc gaccagacgg aatagcgtcc
    12601 gcccacccag tcgtacatcg caaacacgcg ttccgccgca atgccgaaac tttgcgccgc
    12661 ttccgtatcg gcagacaccg cgcaaaaatg gcgcgccgtt tcggattccg agaaccccgc
    12721 accgcgatac cacgccttga ccgcctctgc attgagcagg gtttccggtg ttttaaagga
    12781 cttgctggca acgcaaaaca ttgttgtttc ggggttcaga cggcataaga cctcatccag
    12841 gcaggcaggg tcggcattag aaacaaaatg gacggaaatc tgccgtctga acggctcgag
    12901 cgcctgcacg cacattgccg gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc
    12961 cgcaatccgt tttccggtta tcccctgata taaaccgtcg tccaaactgt gtgcaaactt
    13021 caacgcacga tttaactcgc ggcggatttc gggcaacacg tccctgccgt ccgcataaac
    13081 ggcatccgca ccgtcgggca ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc
    13141 gttgactttc gcacccgtcc gcaaagcacg cattttccct tccaaatccg ccgtctccgc
    13201 aagacggcag agcagttgca gcgtatcttc gccaaaacgg tttttgctgt aatcgaacaa
    13261 catcccgtcc aaacgctcat gcatacgctc aaaacggtcg ggttcggcag caaagcggtc
    13321 gcgcaaaagg atgtggcacg tatcctgata atggcgttcg agcgcatacc atgcacgggt
    13381 aaaggcatcc atctgttttc cttgattttt cagaaccgga ttaaaatata gcagaatgta
    13441 gtctaacaaa cggcagacac tttagcgaat ctcaggaaca ccgcccgccg cgtccaataa
    13501 caccttattt ccatatggcg aaaatatagt gaattaacaa aaatcaggac aaggcggcgg
    13561 gccgcaggca gtacggatag tacggaaccg gttcgcttgg tgcttcatca ccttagggaa
    13621 tcgtcctctt tgagccgggg cgggccaacg ccgtaccggt ttttgttaat tcactatacc
    13681 ctccggccgg catttgaccc gcaacaatat cctgcaagat ttattgtgtg cgcataaatg
    13741 ccggacagcc gcctaaatta aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc
    13801 aggcgggatg tcggcggttt tcatcttacc gggaacacgc cgatactgtt ttttaacgat
    13861 gtttcttaca ttttattcca attactttac ggggctagaa tatggctaaa aatgggggat
    13921 tttctttgtt cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tctgcctccg
    13981 acaaactggt caacggcgaa gtatccgcgt ttaccgaaga agaggcacgc aaaaaactgg
    14041 caaaacgcgg catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa
    14101 tcacacaaga agacattacc gttttcaccc gccagctttc cacgatgatt aaagcgggcc
    14161 tgccgctgat gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg
    14221 aaatgctgat ggaaatccga ggccaagtgg aacagggcag ctcgttgagc cgcgcattct
    14281 caaaccaccc gaaatatttc gaccgcttct actgcaacct ggttgcggcg ggcgaaacgg
    14341 gcggtgtatt ggaaagcctg ctggacaaat tggcaattta caaggaaaaa acccaggcca
    14401 tccgcaaaaa ggtaaaaacc gcgctaacct atccggtatc cgtcatcgcc gtcgccatcg
    14461 gtttggtatt cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata
    14521 tgggcgcgga gcttccccca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct
    14581 catacggctg gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga
    14641 aggcgcgttc gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt
    14701 tcggagacat tgtccgcaaa gggacgattg cccgctgggg caggacgacg gcaacgctgt
    14761 ttgccgccgg cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt
    14821 taatctatga agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ctgtctatga
    14881 cttcggggat gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcctccatcg
    14941 gcgaggaatc gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg
    15001 aggtggacaa tgcggtcggc aggctgtccg ctatgatgga gccgattatt attgtgattt
    15061 tgggcttggt catcggtacg cttctggtcg ctatgtatct gccgctgttc aacttgggca
    15121 acgtggtcgc ctgatttgcc gcacagatcc ggcgcggatt ggttctgcgc cggtttgttt
    15181 ttgctttgaa tatatcaagg acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc
    15241 gtgcctttgg cagcagtttt ggggctgctg gtcggcagct tcctgaatgt cgtcatttac
    15301 cgcgtacccg ttatgatgga acgcggctgg acggtatttg ccaaagaaca tttaaacctg
    15361 ccgctgaccg acgatgaaag ccgtaccttc aacctgatga agccggattc ctgctgtccc
    15421 aaatgccgtg tgccgatacg cgcgtggcag aacatcccga ttgtcagtta cctgctcctg
    15481 cgcggcaaat gcgcttcctg ccaaaccaaa atcagcatac gttatccctt aatcgagctg
    15541 ctgaccggcg tattgttcgg gctggtcgcc tggcaatacg gctggtcttg gattacgctg
    15601 ggcggtttga tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa
    15661 tacctgcccg actcgatgac attacccttg atctggctgg ggctgatatt taatttggac
    15721 ggcggcttcg tgcctttgca gtctgccgtt ttaggtgcgg ttgccggcta tagttcatta
    15781 tggctcttat gtgcagtgta taaactgctc acaggaaaaa ccggtatggg caacggagat
    15841 ttcaaactga ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt
    15901 tttgtttcct ctctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcgg
    15961 cattttgcct tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat
    16021 tccgtatggc gggcggtcaa ctggtggctg acccatccgg tgagatgaca gcatgggtcg
    16081 gactgaccgg cggaatcggc agcggcaaat cggcagccgc gcaatatttt gccgatttgg
    16141 gcgtgccgcg catcgatgcg gacgcggcgg cgcactcgct gacggcttca gacggcatcg
    16201 ccctgccgga aatcaggcgg ctgttcggcg acaccgtttt cgacacacag ggtttgttgc
    16261 ggcgcgacat attgcgtaaa gaaatctttg cctccccatc gcgcaaagcc ttgctcgaat
    16321 ccgtgatgtt gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc tttaccgatg
    16381 cggtttacgg cattgtcgaa attccgctgc tgacggagaa gcgtcaattt atcagcctga
    16441 tacggcgtgt cctgaccata agcgcacctt tggaaaaacg tatcggcagg gtaatggcac
    16501 gcagcgggct gacgcgcggc gaggtggcgg acatcatcag ccatcaggca tccgaatccg
    16561 aacgcctgct gcttgcagac gatgtactgc tcaatgacgg cagcctcaaa agcctacgtg
    16621 agaaaacaat gctcctgcac gcgttttatt cagggatttt cgcctcaaaa ccaacacaag
    16681 gaaaacacaa tggctgaatc gcggcaaaca cgccttcaag tcaaatgtcc gacctgtcaa
    16741 acggcagtag tatggaaacc ggaaaacgca ttccgcccgt tctgttcgca acgctgcaaa
    16801 ctgatcgact tgggcggatg ggcagacggg aaatatacgg tttccggcca aacggaaagt
    16861 ttgccggaaa tatccgaacc cgacggggca taccgctgac cgccccgcct tcccggcaaa
    16921 caccctgaaa ggtcaaatgc cgtctgaaac aaacacgctt cagacggcat tttcattctc
    16981 aaacctaatc gttggtattt gccgttacct cttccaatga agtaatgccc tgcataattt
    17041 tcaaaatacc ggcccggcgc aaatccacca taccctcctt ataggcaacg tccaaaatac
    17101 ccacttccgt accgttgttc ataatcacac gctgcatttc ttcgctgatg ggcataacct
    17161 catacacgcc cgcacgcccc ttataaccct gcccccggca acggtcgcaa ccgacggcgc
    17221 ggtaaagttt ccaatctttt gcaagatcct catcggtgaa accgacttcc ttcaaagcag
    17281 aggcagacgg gcgttccact tcctgtttgc agctcgaaca cagcctgcgt aaaagacgct
    17341 gcgccataat caggctgacc gaactggcaa tattaaacgg cgcgacaccc atattcagca
    17401 tacgcgacaa cgtcgccggc gcattattcg tgtgcagtgt ggaaaacacc atatgccctg
    17461 tttgtgccgc cttaatcgca atatcggcag tttccaaatc acgaatctca ccgaccataa
    17521 tgatgtccgg gtcctgacgc aggaaagact tcaaagcagc ggcaaaagtc agaccctgct
    17581 tatcattgac gttaacctga ttgatgcccg gcaggttaat ctcggcaggg tcttccgccg
    17641 ttgcaatatt taccgactcc gtattcaaaa tattcaaaca ggtatagagc gacaccgtct
    17701 tacccgaacc cgtcgggccg gttaccagca ccatcccgta aggacggtga atcgcttcca
    17761 acaacaattt tttctggaac ggctcaaaac cgagctggtc gatgttcaaa gacgcggcat
    17821 cggaattcaa aatccgcatc acgacctttt cgccaaacag cgtcggcaat gtgctgacac
    17881 ggaaatcgac aggcttgccg cccttttgaa aggtcagctg catcctaccg tcctgcggta
    17941 tccgtttttc ggaaatgtcc aaacgcgaca ttaccttaat ccgggaagca agctgccccc
    18001 ttaccgcaat gggcggctga accacctcgc ggagctgccc gtccacacgg aaacggatac
    18061 gcgcattgtg ttcgtaaaac tcgaaatgga tgtcggatgc cccgctacgc aaggcatccg
    18121 acaaagtttt atggataaac ctcggaacag ggccgtcttc tgcctcctcg ttgtcgatat
    18181 acagggtgtg gctttcctct tcctcttgcc cctccccaag ctcctgaagc agcgatgtcg
    18241 aacgcgaacc cacccaatcg agcaaacccg ccaactggtc atcctcgaca atgaccaact
    18301 caaccgcaat ccctgcggca gaaacggttt tctgaatttg cggcatctgg gtcggatcgg
    18361 aaaccgcaaa aaatactttg tcgccccgac ggaaaaccgg cacacagtgg aactccacca
    18421 tctgctcctc cgtcaacacc cccatcagca ccctgtggcg cggataatga cgcaaatcaa
    18481 gaatcgaata actgaacacc ctcgcaatca atgccgcaag cgacttgggc gaaatgacac
    18541 cgtctgaaaa cagcatcggc aacacttcct tacccgcctg cgactcattg tagtaatgct
    18601 cggcctgctc aacagtaacc acctggtttt gaaccagaat cctcagcaaa cctacgctca
    18661 tacgacctta tccccaaatt tattcattgt tatacctgta cagattttat cataaaacaa
    18721 cagatttcca agcctgaaca tcttttacga agcctgaaaa tcatttcacg attctgccgt
    18781 cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca gatggcaaaa aagccctcca
    18841 gcactgaaag gccttatatc ggaaacttcc cgcaacacgg gaaacagaca aatgcaaacc
    18901 gtcaaacctc gccaacagga atcgaacctg tattttacgc ttaggaggca tacgttctat
    18961 ccgttgaact atggcgagcc gaaatgaaaa gggagatttt aaacctttcc ggcaacaaaa
    19021 gacaagatgt cggccgcaat cagcggcccg acagggatac cgcccataaa tgccaccccg
    19081 atgaccgtac cgatcagcaa gcccgttacc aataccggct gccgtcccat caaaggcacg
    19141 ccgcatcccg ccagccaagc cacgaaaata ccgataaaaa cggcggatat cattttaaaa
    19201 ttcaaaaatt cggcaacagg aggaacctgc gcctttcctg aaaccaacgg actcaaaacc
    19261 cctatggtca aaagaatgat accgaggttc aacccgtgct tctcgaccaa cgggacaaac
    19321 tgtaccaatg ccgtctgctg catcagcagc aatatggttg ccgagacggt aatcgaattg
    19381 ttgttgctga ccacccccaa cagaatcagc gtaaccagaa acaggggaac aaaactgaaa
    19441 ttcatcggtt acgacaccct gaccaaagat tccttcgcca gctgaatcat agcatccttc
    19501 acttcgctgt cggacagggc atccaacgcg gcaacggcac aatcgactgc tttgcgcgcc
    19561 tcgcctatcg aatatgccaa agcatccgaa cggacgacat aatcgcggat tttttcaaaa
    19621 tagccgcgat ctgcattttc caaagcagta cgcacatcgt tcgcaacctg ttcggaaccc
    19681 tgacgcatca gataaatcaa aggcagggtc ggttttcctt ccgccaaatc gtcgccgacg
    19741 tttttacctg tttcttcggt ttcccccgaa taatccagca catcgtcaat aatctggaat
    19801 gccgtaccga cgtacatacc gtagtctttc aaggcctgtt cgtgcccggg ggaagctttg
    19861 cccaaaattg cgccgacttg agcggcagct tcaaacaatt ttgccgtttt atattggatg
    19921 actcggatat attgttcttc ggtaatgtcc gtattgccga tgttcatcag ctgcatgacc
    19981 tctccctcgg caatgatgtt ggtcgcatcc gccatcactt ccaaaacgcg catactgccc
    20041 gaggcaacca tcagttgaaa tgcacgggta tataaaaagt cgccaaccaa cactgccgcc
    20101 gcattgccga acagattgtt tgccgttgcc cgcccacggc gcaaatcgct ttcatcgacg
    20161 acatcgtcgt gcaggagtgt ggaagtgtgg ataaactcga ccatcgctgc cagcgaatac
    20221 agtttctcgt catcataacc gaccgcctta cccgccaaaa tcgtcataat cggacgcagg
    20281 cgtttgccgc ccgcgctgat gatatatgta ccgatttgcg aaatcagtgc gacatcggat
    20341 tgcaccgcac ggttgatgac ttcattgact ttggcaaggt cttcaggcag atgtcgctgg
    20401 aaatagggca gattctcgag catggcagac tctcaggttt acgtttccgt caaacggcag
    20461 ataccgcttg aaagctgatt caaatagcgg gcaattatat cagcaaagcc atcagggtac
    20521 atcttttcag acggcatatg attgcccgaa ccgcgcaaac tttgacaaaa caggcaaata
    20581 acaatacaat gcccaatttc gcacaatggt gtgcggatat taaccataac ccttatggag
    20641 ttgagtatgt acgcggtcgt aaaaaccggc ggcaaacagt ataaagtttc cgtcggcgaa
    20701 aaattgaaag tagaacagat accagcccaa ctcgacagcc aaatcgaact gaccgaagtt
    20761 ttgatgattg ctgacggcga atctgtaaaa gttggcgcac cctttatcga aggtgcaaaa
    20821 gtaacggcta aagtagtggc acacggtcgt ggcgaaaaag tccgcatctt caaaatgcgc
    20881 cgccgcaaac actaccaaaa acgccaaggc caccgccaaa atttcaccca aatcgaaatc
    20941 gtggcaatcg cctaacctta aaacttagga gcattacaaa tggcaagtaa aaaagcaggc
    21001 ggcagcaccc gcaacggtcg cgattcagaa gccaaacgct tgggcgttaa agcctacggc
    21061 aacgagctga ttcccgcagg ttccatcatc gtacgccaac gcggtaccaa attccacgca
    21121 ggcgacaacg taggtatggg caaagaccac actttgttcg ccaaaattga cggttatgtc
    21181 gaattcaaaa ccaaaggcgc gctgaaccgt aaaactgtca gcatccgtcc ttacaccggt
    21241 tctgaagaat aattcaaccg atacctgaag ccgcatcttt tcacgatgcg gctttttaca
    21301 taccccatac ccgatttgat acacattaag ctgaaagtaa aaatccgcat acaccctccc
    21361 ctgcatattt cttcaacaac gggttttgat ataatcgcct atctgttaca gatagttcaa
    21421 acggcattca aacccttaca aatgccgtct gaaatcatcg gcatccgcct atgcaaagga
    21481 tattttatga gtttacacag cgacatcctc gtcgtcggcg caggcccggc gggtttaagt
    21541 tttgcagccg aacttgccgg cagcggtttg aaagttactc tgatcgaacg cagcccactg
    21601 actgttctgc aaaatcctcc ctatgacgga cgcgaaatcg ccctgaccca tttttcccgc
    21661 gaaatcatgc agcgtttggg tatgtgggac aaaattcccg aaaacgaaat ctaccccttg
    21721 cgcgatgcca aagtgctgaa cggacgttcc gactaccagc tccactttcc ccaaccgact
    21781 gaagcgcgcg gcgagcctgc cgactgtttg ggctatctga tttccaacca caatatccgc
    21841 cgcgccgcct atgaaatcgt atcacaactc gacaatgtca gcattctgac cgatacggcc
    21901 gttaaagaag tcaaaacatc tgataatgaa gcgcaagtct ttttggaaaa tggagacatt
    21961 ctaaccgcgc gtctcctttt ggcagcagac agccgtttct cacaaacccg ccgacaactc
    22021 ggtatttctt cagatatgca cgactacagc cgaaccatgt tcgtctgccg catgaaacat
    22081 accctttcca accaacatac cgcttacgaa tgcttccatt acggacgcac catcgcactg
    22141 ctgccactgg aaaagcgcct gaccaatacc gtcattacag tcgataccga taaaatcaac
    22201 agcgttcaaa acctttcgcc cgaagaattg gcggcaagcg taaaagaaca gctcaagggc
    22261 aggctcggcg acatggaact ggtcagcagc atccaccatt acccccttgt cggcatgatt
    22321 gccaaacgtt tctacggcaa acgcagcgcg ctgatcggcg atgcggctgt cggtatgcac
    22381 cccgtaacgg cacacggctt caacctcggg ctttccagcg cggatattct ggcaaaactg
    22441 atactcgaag ccgaacagcg cggtcaagac atcggcgcgg cttccttgtt ggaaaaatac
    22501 agcagcaaac atatgctgca cgcccatccg ctttatcacg gcaccaatat gatgctgaaa
    22561 ctgtttacca acgaaaccgc gccggcaaaa ctgttgcgcg gtttggtatt acgagccggc
    22621 aacaacttcc cgccgctgaa aaagctgatt accaaacaac tgaccggttg aaaactgccg
    22681 aaatgccgtc tgaaacgttt cagacggcat ttttgtatta tgaaccgttt tgcggtttcc
    22741 atatccttgg cagacggttt gcagcagatt cgcatacggc agatgtttca agcagaaagg
    22801 aaacacaata aaaataaaaa agcctccgaa cagtcggagg ctttgctttc aaactgaaat
    22861 tatttcagtt tggtttcttt gtacactacg tgtttgcggg caaccggatc gaattttttg
    22921 atttccagtt tgccgggcat agtgcgttta tttttggtag tggtgtagaa gtggccagta
    22981 cctgcaccgg attccagttt gattttatcg cgcattgcag taatccttaa ttaaatagtg
    23041 tttaaattaa gcttcgccgc gagcacgcaa atcagccaat acgacatcaa tgcctacttt
    23101 gtcgatggta cgcaatgcag cgttggaaac gcgcaggcga acccagcggt tttcactttc
    23161 tacccaaaaa cgacgtgatt gcaagttggg caaaaaacgg cgtttggttt tgttgttggc
    23221 gtgcgatacg ttgttgccgg acatcgggcg tttaccggtc actttgcaaa ctcgtgccat
    23281 aatcagtctc caaacttcta aaatagtaaa acgcgattta taccacgaaa aacaatgccg
    23341 tttcaagtgt tttaaagaaa aacagatgtt ttgcgcgggt aagccgcccg agtgccgcat
    23401 ctgcggaacg tttgcaggtg cggaacagat ggggcgcgat tatagcggat ttcgggagct
    23461 tctgcattca aaatgccgtc cgaacatatt tccagtattt tcagacggca tttcaagttt
    23521 tcaaatccct caatgtccac cgccgccgct gtggaacggc ggtttcgcca gccagacaat
    23581 gggaatcagg acgataaaca aaatgctgcc ggcgaggaat atttcgttcg atccgatgat
    23641 gaagccctgt tgcgtaatgg tattgttgat gatgcctaga gtttgaccgt cggaaatgcc
    23701 ttgttgggac aaatgcgccg ccgtttcgtg caatgttgcg gaatagggcg tgatgtgttc
    23761 ggcaaaacgc gtgtggtgca acgcttcgcg ccgttcccac agggtgctga cgacggatac
    23821 gccgacaccg cccatcagca cgcgcaggaa attcgacagg ctgccggcgg cggcaatctg
    23881 tccgcccttc atgtgcgaca gtgtgatggt ggtcagcggc aggaagaaca tggcaacgcc
    23941 taccccctgc caaaactgcg gccagatgac gttgccgata tccatgtcgg cataaaaatc
    24001 agtgcgccaa taaaaagtga aggcaaaggt caggaagctg gcggttacga gcaggcgcat
    24061 atcgatttta ttgccgaacc tgccgattaa cggagatagg aagacgggga ggatgccgac
    24121 gggcgcggca gcaagccctg cccacgcgga ggtatagccc aagttggtct gcaacactaa
    24181 cggcagcagg gtcagcgtcc ccatatacac catgaaaccc aacgaggtgg caattacgcc
    24241 gacggtaaaa ttccgatctt taaacagcga taaatcgaca atcggatatt tttctcccaa
    24301 ttcccaaaca ataaaatacg acaagcacac cagtgcgact acgcccaagg taatgatttc
    24361 tccagaggcg aaccagtcaa gttccttacc cctgtccagc atcatctgta acgcgccgat
    24421 accgaccatc atcagcgtca gcccgacata gtcggtcggc gttctgacgg ttgccgtttc
    24481 gcggtgtttc aaatgtttcc atgtaatcca tgccgatatg atcccgatgg ggatattaat
    24541 gaagaaaatc caaccccaat gccagtttcc ggaaatccaa ccgccgagta tcggcccgag
    24601 gacaggggcg acaacgacgg tcattgccca caatgccagt gccagcatcc gttttgcggg
    24661 cggataggat gccattaaca ggctttgcga caggggaatc aacggcccgg cgataaagcc
    24721 ctgcaaaatg cggaaaacaa ccagcgactg aaggttgggc gcgatgccgc acagccacga
    24781 tgcgatgacg aaaccggcgg cggcggcggt aaacaatttg acctcgccga tacgttttgc
    24841 caaaaagccc gtcagcggca cggaaacggc gtttgccacg gaaaaagaag tgatgaccca
    24901 cgtcccctgc gtggttgccg caccgaggtt gccggcaatg acggggacgg cgacgttggc
    24961 gatggtcgta tctaaaacct ccataaatac ggcaagcccc aaagacagcg taacccacgc
    25021 caatgccgca cccttaagcg gtggataatc cattgtgtcc tccgtttcag acggcatcag
    25081 ggcgcggatt gcccgaggat ttcatcgacc gtccgatcga cttccgacca gtcggtactt
    25141 tccatttccg gcaatgccgc acccggcgtt tttgaaacag gcgcgcctgc ggcggaagta
    25201 tccactttaa ccgtcatcga caaaccgata cgcaacggat gcctgtccac atcttcgcgg
    25261 ttcagcacga tacggacggg gacgcgctgc accactttaa tccagttgcc cgttgcgttt
    25321 tgcgccggaa tcagcgaaaa cgcgctgccc gtacctgccg aaaaccctgc caccctgccg
    25381 cgataaacaa tttgtttgcc gtacaaatcg gacaccagct cggcaggctg tccgattttc
    25441 atatgccgca actgcgtctc tttaaaattg gcatccaccc acacatccga cagcggcacc
    25501 accgccatca gcggcgcgcc tgccgccacc tgctgcccga cctgcaccga acgcttcgcc
    25561 acctgaccgt ccgccggcgc gcggacttgc gtccgccgaa ggttcagcca cgcatctttc
    25621 aacctgccga ttgccgtctg aacctccggc tgttcgcgca aagaaacatc tccgcccaaa
    25681 gccgcgcgtg ccgaagattc ttccgccaaa gccgccttaa ccgccgcctg cgcctgagac
    25741 actgccgtac gggcgtgtgc cagctcttcg gcggacaccg cacccgattc cgccaaagcg
    25801 gaccggcggc gcaaatcatc ctgcgcgcgc gccaaatccg cacggcgcaa ggcaacctgc
    25861 gcccccgcct gcgaagtggc ggcattttgc cggcggtttt ggcgcaccgc ctgaaccaac
    25921 tcgttttttg cccgctcgta agccagcaca tcattatcgt catccaacac cgccagcacg
    25981 tcgccttttt tcacggcatc cgtatcgtcg tgcaaaacct tccgcaccgt gccgcccttt
    26041 tgcggcgtaa cctgaaccac gcgtccggca acataagcgt cttccgtttc ctcttcgtgc
    26101 tgccaccata aaaaaaacgc cgatccggcg gctgcggcgg aaagcgcgaa cagcagcgtc
    26161 aatgccgtca ggcggcgttt gcgtttgact tgcgtgtttt gaagttttgt ttcgtctgtg
    26221 tgcgtttcca taagattggg ttccgaattt cctgatttca tataaacaat gttctttcaa
    26281 taggaaatag attttatatc cgcgcaataa ttcaatcaag tcagtatttt agatgcaata
    26341 ttcaaaaaac aacgcttcag gcggcaaaac tgaaaatata tttcgtttat atatgacatg
    26401 gcaaacaccg ccgcccgcac catgccgtct gaagaaaact acacaaatac cgccgcttat
    26461 attacaatcg ccgccccgtg gttcgaaaac ctcccacact aaaaaactaa ggaaacccta
    26521 tgtcccgcaa caacgaagag ctgcaaggca tctctctttt gggtaatcaa aaaacccaat
    26581 atccgaccgg ctacgcgcct gaaattctcg aagcattcga caacaaacat cccgacaacg
    26641 actatttcgt caaattcgtc tgccccgaat tcaccagcct ctgcccgatg accgggcagc
    26701 ccgacttcgc caccatcgtc atccgctata tcccgcacat caaaatggtg gaaagcaaat
    26761 ccctgaaact ctacctcttc agcttccgca accacggcga ttttcatgaa gactgcgtca
    26821 acatcatcat gaaagacctc atcgccctga tggatccgaa atacatcgaa gtgttcggcg
    26881 agttcacacc gcgcggcggc atcgccgttc atccgtttgc caactacggc aaagcaggca
    26941 cagagtttga agcattggca cgcaaacgcc tgttcgagca cgacgcacaa taaaccatcc
    27001 ttcccatatt tcagacggca ttccttcgat acggtatgcc gtctgaacgc ctttccgaaa
    27061 ggtttttatg tacgcattga ccgccgcaca gcaacagaag gcactcttcc ggctggtgct
    27121 tttccatatc ctcatcatcg ccgccagcaa ctatctggtg cagttcccct tccggatttt
    27181 cggcatccac accacttggg gcgcgttttc ctttcccttc atcttcctcg ccaccgacct
    27241 gaccgtccgc attttcggtt cgcacttggc gcggcggatt atcttttggg tgatgttccc
    27301 cgcccttttg ctttcctacg tcttttccgt tttgttccac aacggcagtt ggacgggctt
    27361 gggcgcgctg tcccaattca acacctttgt cggacgcatc gcgctggcaa gttttgccgc
    27421 ctacgcgctc ggacaaatcc ttgatatttt cgtattcgac aaattacgcc gtctgaaagc
    27481 gtggtggatt gccccggccg catcaaccgt catcggcaat gcactggaca cgttagtatt
    27541 ttttgccgtt gccttttacg caagcagcga tgaatttatg gcggcaaact ggcagggcat
    27601 cgcttttgtc gattacctgt tcaaacttac cgtctgcacc ctcttcttcc tgcccgccta
    27661 cggcgtgata ctgaatctgc tgacgaaaaa actgacggcc ctgcaaacca aacaggcgca
    27721 agaccgcccc gtgccctcgc tgcaaaatcc gtaaaacagc tctgtaggga atcgggacgg
    27781 ttgggttttc ttttatttgc aaacactgcc ggaacggata aatccggatt cccacccact
    27841 tcgtcattcc cgcgaaagcg ggaatccggt gcgttggggt tcagtcattt ccgataaact
    27901 cctgccgcat tgtatttcta gattcccgct ttcgcgggaa tgacggcgga tgggtttccg
    27961 tgccgtggtg tccggattcc cgccggtacg ggaatgacgg cagcgcttcc ttggcttagt
    28021 tttattttaa gccactataa atgccgtctg aaaacctgtt tgcagatttt cagacggcat
    28081 tgttttcgtc gtcggttcaa acttcaatcc gattgcgaca cggtaaccgg tatgccgcgc
    28141 aagcgcgcaa gcagcgcgtc cgccttctgt ttctgcgcgg caaacgccgc cttgtccgcc
    28201 tgcgtcaatt cgggtgtcgg caatgcgacc gaaacaggat tgacgggctg cccgttgatg
    28261 cgcgcctcgt aatgcaggtg cggcccggtc gaacgccctg tcgaaccgac aaaaccgatg
    28321 acctcgccgc cgcgcacatt gccttgtgcc tgcgaaaacg cgctcaagtg cgcgtacagc
    28381 gtttccacac cgttggagtg gcgtatcatc accgcgttgc cgtatccgcc cttccggcct
    28441 ttaaaggtaa tcacgccgtc ggcggaagcc ctgaccggcg ttccctgcgg tgcggcataa
    28501 tcgatgcccg tgtgcagccg ccatgtgtgc aggatggggt gcatacggta gccgaacggc
    28561 gaagaaatgc gcgtatagac cagcggctcg atgttgaagc cgcctttttc ctgcaacacc
    28621 ctgccgtctt catcgtaata attgccgccc cctccgcctt ccttgtccga acggtaatag
    28681 aacgcctgat gggttgtgtc gcccttgaca acttccgccg ccaaaatatc gcccgccgcc
    28741 acctgctgcc cgtggaaata caggctgtcg taaagcaggc gcacggcatc gccttccttc
    28801 aaaccgtcaa ggctgaagcg gccggcaaaa atcccgctta aggattcgcg gatttcgacg
    28861 ggcacttccg cccgcgccag cgaaccgcgc gccgacgttt tgaccacgac cgaacgcagt
    28921 gtcggcaaaa ccttcatatc cgcatcagaa gccgaccgcc gccatatgcc gccttttttt
    28981 tccaaagcga ccagattgcg ctcgccgtct tcgtcggtaa aaaactgcac ttcgcgcgca
    29041 ctgccgtcgc cgccgaccaa aacatgaacc gactggtcgg cacgcaaatg ccgcaaatcg
    29101 gcttcgccgc catatttttc cgtgattcgg gcaatctcgt cccgcgccat acccgaacgc
    29161 gccagcacgt ccgccagcga gtcgcccggc tgcaccgcct cctgcaccca atatgccgtc
    29221 tgaacgccgc tgccgcccca agacagcggc ggcagttttt gttccacgcg ctgcggtctg
    29281 acgcgctccg tcccctctgt cgaagcaatg tatgccgccg acaccagaat aatcgaaacg
    29341 gcaagcgcgc gcagcgcgta tttccgatgt tttgccgaaa gtgggaagac agccatattg
    29401 ttcctttttg acggcgtttg cgccgaatcc tctataatat ccgcccgatc ctcatttgtc
    29461 aggcatcaaa cgatgaaact caccctcgcc ctcccctcgc tcaatttgga tgaagacgaa
    29521 atccgaatac ctctatgcct gcccgccttc aacaaaatcc tgcaatacgg ctcgccgcac
    29581 cgccaaagct gcacggcttc ggcattttac gcacgttatt tgtggtgcgg aagactggca
    29641 caacgcccgg cgcaaagcct gaatatgccg tctgaaaccg tcgcgctcgc cacgcccgtc
    29701 tggcaaaaaa tggggctgca tcaggcaaat gtgctgacgg cggaatattt ggatgtcggt
    29761 acggatgaag cggaacggct ctgccgcgat ctgtcggcgt tttacggcga cattccgtgg
    29821 cgtttcgttc ccgtcctgcc cgaattatgg ctggtttccc ttccccgcgc gtaccgctgg
    29881 ggcgcgaagc ccgttttgga cttgggcgga ctgttgggcg cggacgatca gccggacggg
    29941 gaagacgcgt tggaatggct gcgcgtccaa accgaaatcc aaatgtggct gaacgctcac
    30001 cccgtcaacc acaaccgcaa aaaacgcggg ctgcccgaat tgaacggctt atggctttgg
    30061 gacagtctgc acggaagcgc acaaggcggc acgcttttcg ccgatacggt ttggagccgt
    30121 tttcacccga accgccgagc attgcccgac agcttccgag cttatgcgga aacggcggca
    30181 cacctgcccg atacgcacca catcctgttt atggacgatt tgcgcctgac cgccctgaca
    30241 ggggacaggg agcggtatgc ggcaatattg cagcagtggg aagaacgctg gttcgcaccg
    30301 ctttacgaag ccgtccgcac cggcaaaatc aaacggctgg acatcgccac ggacggacaa
    30361 cacggcggta cgctgacctt caaacccaca gacaggcgga agttttggcg ctgcacgaaa
    30421 acctttgacg gaatctggta aacccgcccg ccgcaaccgc gacaatgccg tctgaagccc
    30481 gaatcgggtt tcagacggca ttttaccccg catcgccgtt atgccggaaa aaccggaata
    30541 cgtttccgca cacgcggcgg cacgcaaaac accgcccgaa agccgccgat gcccgccgtg
    30601 acaccgccgc cggcagggtt tccgccgaaa aggaacagca atcagaacgt tttgttaaac
    30661 tcgacaaacg ccctgttttt ctcgtattcg ttaaacacat cgttgctcca cgtttcgcgg
    30721 tgcgacagcg tcaggcgcgg cgtgatgcct ttgaaatgca atgcccggtg ccaaaggctc
    30781 aaggatgtgt ccgattcttt atccctgcgc ctttcccctt taaaactgct gaagaagccg
    30841 ggtttttcat aatgccgttt cgccacgccg aggcggaaca gcgaagacag gccgctgccg
    30901 ccccattcct gcccccaggc aaagcgcagg ccgtaacggt tgaaattgtc gccacggtcg
    30961 gcggggttgc gctcgcggta aaaatccaaa ccgcccgtcc aatattggcg cgcattccgg
    31021 taaaacacca gcgaattgga aatttgcaaa tgggtattgt cggaacgcgc ccggcgcgta
    31081 ttcttcaaac gcccccactc cgccgaagac agcgtttgcc atctcggggt ttgccaacgg
    31141 ttgaaataaa ggcgtgcgcc gttggcgtaa gaataagcgt cgttgccgta ggtgcggcgt
    31201 tcgtggaaca ctgccagccc gacatcttta cgccggtcgg caaaaccgat gccgccggaa
    31261 acacctgccg tcatatcgtt gaatttctta ttccccggat aagccctgcc ggacacgtcg
    31321 ccgcccgccg tcgtgtacca gccgtttttc agcgaccatt ttttctccgc gccgaaccgg
    31381 taattgaccg ccgtgccgtc cacctgtttc gggaaagtcc agttgccgta ctgctgctgt
    31441 ttcggggctt ggttgatatt gtgttcgcgg gtaatgctga aaccgccgtt taccttccac
    31501 gcatcgcgtt cgcgcaatgc cttgcggtac agctcgacct gctccataag ctgcggcggc
    31561 agatcttctg ttttcaggcg gtcgaactgg tctgccgccg cctcgttctg cctgtcttca
    31621 aacaatgccg ccgccaaacg catacggacg gcgggcgcgt cgggttgggc ggcaatcaat
    31681 tcccggtaat gggaaaccgc ctccttcacc ctgccctctg cctgcgccaa aatcccttgt
    31741 gcataaagtg ccaacatctt atcctgccgc gcctgttgta ggtaaatcgg caaaatcacg
    31801 cggataccgg caatattgtt tgagaccact gcggaataca tggcacgcga caacaattcg
    31861 ggatttttca gcagggtttc gccgtcaacc tgcaaaactt ttcctttttc ccgcacctgc
    31921 cccggtacct tctccctgtc gatcggtttg acctccgctt catgaagcct gaactcggga
    31981 cggcttctca aatccggttc gcgcggtgtt tcttcggcgt atgccgcgct tgccaataaa
    32041 ggcagcagca tccatctgtt tcgtgcaggc ataaatgtct tcccacaaaa ataaaaataa
    32101 aaaataacca ttatcaaccg gcggattgta tcagtttttt atgccgctgt tccaaaccct
    32161 gccgcccgat gccgcgacaa tgccgtctga agccagaatc gggtttcaga cggcggcaaa
    32221 aaaccggcgg ttttccgccg gtttcatcat tatgccccgc ccttcggggc ggcagggttc
    32281 ggatcggttt gcggggcggc gcacggggcc gtgcaggtgt gccgcctgtg tgtcaaaaca
    32341 tttcccggcg catcaaaaca accatttgac cgacaatgcg gcttctttgt cgccgtccgt
    32401 ccttttgccg tatccgatgc gtgcggacat atggcctttc caaccggctt caatgccaaa
    32461 ccgcccttcg agcgccgtcc tgcctgccag cgtctgtttt tcgccgtcca tttccatgcc
    32521 gaaagatttt gacctgtgca aaacattaaa agcggcaaaa ggctgaagat tgacaccgtt
    32581 acgcaaagca aaacgggttt ttgcccgaat gccggcgcgg atttgccact gaccgctgcc
    32641 gagcagcccg accgccgccc ctcgctgtcg gtaaagccgc cgtttacgcc caagtaggta
    32701 aactgcgcct gcggttgcag gtaaaactgc atattactgc cttatatatt gcgtccctaa
    32761 gaaggggcga ttaacaaaaa ttaacgcccc ttcttaggga cgcaatatat aaggagtgag
    32821 tacctgttct tctattaaag aattaatctt ttgaggcatt ttctgttgaa gtttattaaa
    32881 cacatcttca gaaattccaa actgctttat tatctcttcc ctaacccgtt ttttattttc
    32941 ttcccaagcc tcgggtcttg ttttgtataa atcctgtaat tgctttttaa ctgcctgctt
    33001 taaatcagtt cggtaattat ctgtaatata tttcttaaca acaggcatat tatcgacggc
    33061 gaggatgccg ctattgtagt ttttacccgt aatattgatt tcgtcataca aggcttcaga
    33121 tgatttttga tcggttttac cgctggagaa ataatggaaa acaccgtttg ccggcttgtc
    33181 tgaaaatgaa aaatcggcag tatcgccaac atttgtagga cgagtatccg aaccgggctg
    33241 ataactgaaa gcaccgaagc aatcaaaaga cacactgcac gggccgcgcc aaacgtggga
    33301 atttctgtct tccgccgctt ctaaaataaa tttgttttta atctcggctt ctttgacggg
    33361 atcgtacgca tccgaacccc aagcatatgg cgtgcaaaac agcgataaaa cagataatgt
    33421 taatagatta acccgaccgc ttgcttgctt tcagtgtcat gagaaatttt cctttgtcaa
    33481 gtgtt
//
LOCUS       DAWTHD010000009        32486 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWTHD010000009 DAWTHD010000000
VERSION     DAWTHD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32486
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.009"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(158..1192)
                     /gene="purM"
                     /locus_tag="WM704_000325"
     CDS             complement(158..1192)
                     /gene="purM"
                     /locus_tag="WM704_000325"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFA9339834.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGLSVGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
     gene            1433..1621
                     /locus_tag="WM704_000326"
     CDS             1433..1621
                     /locus_tag="WM704_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339835.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            1881..2870
                     /locus_tag="WM704_000327"
     CDS             1881..2870
                     /locus_tag="WM704_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFA9339836.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            3212..3691
                     /locus_tag="WM704_000328"
     CDS             3212..3691
                     /locus_tag="WM704_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFA9339837.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(3753..6800)
                     /locus_tag="WM704_000329"
     CDS             complement(3753..6800)
                     /locus_tag="WM704_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFA9339838.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTCASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISEAAPILLDTIAEYMGKSRAEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLA
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYRIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(6830..6979)
                     /locus_tag="WM704_000330"
     CDS             complement(6830..6979)
                     /locus_tag="WM704_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339839.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            complement(7318..7491)
                     /locus_tag="WM704_000331"
     CDS             complement(7318..7491)
                     /locus_tag="WM704_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339840.1"
                     /translation="MTTAVKPPADLVRPCPKLPHLEGNTGADVLPWSLQVIGLYKDCR
                     ARHGALVRALGAD"
     gene            complement(7475..7816)
                     /locus_tag="WM704_000332"
     CDS             complement(7475..7816)
                     /locus_tag="WM704_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339841.1"
                     /translation="MNGLIKNPKLIAVSVCAVFVAGAWQYDRAAQYRRGYGAAMLEVS
                     ERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADGV
                     RELNAAVDDGG"
     gene            complement(7800..7958)
                     /locus_tag="WM704_000333"
     CDS             complement(7800..7958)
                     /locus_tag="WM704_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339842.1"
                     /translation="MMNPTEFLKARIAEWEAKSKEAGGNADFKAFEFAESEIKNYKAM
                     LKTYERPD"
     gene            complement(7958..8497)
                     /locus_tag="WM704_000334"
     CDS             complement(7958..8497)
                     /locus_tag="WM704_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFA9339843.1"
                     /translation="MKELKWIEEARKYLGAHEKVNGKSNPVLLAMLQEMGNFNQEQKA
                     WWKETDTPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLTAAAATGAQGAGEA"
     gene            8704..8940
                     /locus_tag="WM704_000335"
     CDS             8704..8940
                     /locus_tag="WM704_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFA9339844.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            8940..9287
                     /locus_tag="WM704_000336"
     CDS             8940..9287
                     /locus_tag="WM704_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFA9339845.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(9342..9494)
                     /locus_tag="WM704_000337"
     CDS             complement(9342..9494)
                     /locus_tag="WM704_000337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339846.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGAAPLTALIWKPAD
                     ILNALK"
     gene            complement(9655..9987)
                     /locus_tag="WM704_000338"
     CDS             complement(9655..9987)
                     /locus_tag="WM704_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339847.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(9988..10458)
                     /locus_tag="WM704_000339"
     CDS             complement(9988..10458)
                     /locus_tag="WM704_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339848.1"
                     /translation="MLRFLTERRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(10529..10834)
                     /locus_tag="WM704_000340"
     CDS             complement(10529..10834)
                     /locus_tag="WM704_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339849.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEYIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(10946..15091)
                     /locus_tag="WM704_000341"
     CDS             complement(10946..15091)
                     /locus_tag="WM704_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFA9339850.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLIW
                     YGDFVTIEHKTTTRQGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLRQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKVSGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTEQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            15331..15609
                     /locus_tag="WM704_000342"
     CDS             15331..15609
                     /locus_tag="WM704_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFA9339851.1"
                     /translation="MESQTFASVFDALCDTPAEAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(15637..16068)
                     /locus_tag="WM704_000343"
     CDS             complement(15637..16068)
                     /locus_tag="WM704_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9339852.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(16070..16924)
                     /locus_tag="WM704_000344"
     CDS             complement(16070..16924)
                     /locus_tag="WM704_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFA9339853.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVRIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(16921..17520)
                     /locus_tag="WM704_000345"
     CDS             complement(16921..17520)
                     /locus_tag="WM704_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFA9339854.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(17520..17786)
                     /locus_tag="WM704_000346"
     CDS             complement(17520..17786)
                     /locus_tag="WM704_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339855.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(17798..18127)
                     /locus_tag="WM704_000347"
     CDS             complement(17798..18127)
                     /locus_tag="WM704_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339856.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRREQEAAQA
                     GEARAAD"
     gene            complement(18188..18961)
                     /locus_tag="WM704_000348"
     CDS             complement(18188..18961)
                     /locus_tag="WM704_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339857.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPEQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(18987..19415)
                     /locus_tag="WM704_000349"
     CDS             complement(18987..19415)
                     /locus_tag="WM704_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339858.1"
                     /translation="MNREAVYSALWSKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(19412..19894)
                     /locus_tag="WM704_000350"
     CDS             complement(19412..19894)
                     /locus_tag="WM704_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFA9339859.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQREVKQ
                     NRLSGRVLRVRTDNLRRSIHQRVNVSGNVVSGEVNTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGRPLKPPRYVHVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(19894..20424)
                     /locus_tag="WM704_000351"
     CDS             complement(19894..20424)
                     /locus_tag="WM704_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFA9339860.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(20427..20786)
                     /locus_tag="WM704_000352"
     CDS             complement(20427..20786)
                     /locus_tag="WM704_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339861.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     AAEAAEAAEPAEAEKAE"
     gene            complement(20793..22292)
                     /locus_tag="WM704_000353"
     CDS             complement(20793..22292)
                     /locus_tag="WM704_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339862.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKISVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(22333..23490)
                     /locus_tag="WM704_000354"
     CDS             complement(22333..23490)
                     /locus_tag="WM704_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFA9339863.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVNLSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
     gene            complement(23558..25705)
                     /locus_tag="WM704_000355"
     CDS             complement(23558..25705)
                     /locus_tag="WM704_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFA9339864.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVAGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPGHPNCRCAVVPVLAGDMPES"
     gene            complement(25702..27123)
                     /gene="terL"
                     /locus_tag="WM704_000356"
     CDS             complement(25702..27123)
                     /gene="terL"
                     /locus_tag="WM704_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFA9339865.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(27185..27634)
                     /locus_tag="WM704_000357"
     CDS             complement(27185..27634)
                     /locus_tag="WM704_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339866.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(27927..28754)
                     /locus_tag="WM704_000358"
     CDS             complement(27927..28754)
                     /locus_tag="WM704_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFA9339867.1"
                     /translation="MNNSVQISNISIHQTEIGLFSLNDLHRASGGEDRHAPRRWLQNI
                     QTNDLIKELEKDGKPSIQSKQRLGTFVCRELVIAYGMWISPKFALQVIQTFLAVSDGI
                     KSAKTTADDRTGLRQAVAALVGRKGIDYSSAYSMVHQRFNVESVEDIPAGKLPEAVAY
                     VHALTLHTGLTGEVLDREPLPAPQPALPISGNALYDLAVAVSYGARAIQMGRDVSLPL
                     KQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKIRPILPEIRNLSAV"
     gene            complement(29045..30004)
                     /locus_tag="WM704_000359"
     CDS             complement(29045..30004)
                     /locus_tag="WM704_000359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339868.1"
                     /translation="MAYINILAGDFHKGKAVLKKDCIVLSRGGKVALLDIAGYEVQDG
                     GVIEVVFFDGRRMLVEKNDAFLQAVKVALYNEPQNPEERRQQYNQRQVNSAAKAKKAK
                     KIKLIVGGIFVLLFVAMCAVPKKELTPEEKAAAEKQKIDGKIASGVREFKYDKKAYPK
                     LYKQWGEKAVNEMNGYLPRIAEHIAREDSCDAVETVDISDSRSNPKAKQMVFFVDCRN
                     GKRFFVSTDDLNSGRKSTAEQDKKIDSSAVISQCDEAIKAQLNHPRTFDPHILDTATG
                     VNQNGNVLVTRGFTAKNGLGMQIDYRAYCVITDNKVEVSIEQK"
     gene            complement(30065..30472)
                     /locus_tag="WM704_000360"
     CDS             complement(30065..30472)
                     /locus_tag="WM704_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339869.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GALHLQTDLGLAAWYGAIRQAAGDDFDLLFEKV"
     gene            complement(30739..31284)
                     /locus_tag="WM704_000361"
     CDS             complement(30739..31284)
                     /locus_tag="WM704_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFA9339870.1"
                     /translation="MQNLPQQGSPAKARGKTRGGGSRRDDGGKAARIHPRRARRLPDI
                     GRRPVDATGRGMRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAYRETVRRIAQGAGA
                     MPSEGAVAVYVRLIPKANKDGGANKTVIDLDNALKVALDALQGVAYHNDRQVRRIAAD
                     YAGEPVAGGGLAVEVGELDEK"
     gene            complement(31419..31568)
                     /locus_tag="WM704_000362"
     CDS             complement(31419..31568)
                     /locus_tag="WM704_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339871.1"
                     /translation="MDTLLSIITALSFAGAATLAVWLLVEAADAVLRRKRDGKGEDDF
                     DGFGY"
     gene            complement(31745..32239)
                     /locus_tag="WM704_000363"
     CDS             complement(31745..32239)
                     /locus_tag="WM704_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFA9339872.1"
                     /translation="MTVQNTQTETVRTEAAPQQGGNTNPGYYKNREHECVGFAQYLNF
                     NLGNAFKYIWRHKEKGGREDLEKALRYLERQRADAPKFKKLKHRRYEKMYAGLKDCGF
                     DGGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLLEKMPPEAGRAPHPESPMPPETA
                     GGGI"
     gene            complement(32253..>32486)
                     /locus_tag="WM704_000364"
     CDS             complement(32253..>32486)
                     /locus_tag="WM704_000364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339873.1"
                     /translation="EMREGAECGRAGEALPANQSLRRRGVSDGIGGGSRKKDGGAWGI
                     SPRNAPDLSGKAGKTLWEYPSLPDIEKRVKTQI"
BASE COUNT         6845 a         9916 c         8168 g         7557 t
ORIGIN      
        1 attatatcat tttatagcat tatcgaaata tgatgaagaa gtttaagccg ccataccggg
       61 caaaatatca gtttgcagaa ctgtgttgcg gatacgcgtt tggaagatgc ggaaatgccg
      121 tctgaaacaa attcagacgg catttgatgc ttggaagtca ggcaacttgg gtttgatgct
      181 cgttcccttg acgctctcgg atgcaaccca aacggtacac tgtctcgccc tgcccgctca
      241 ggaatgaccg aaccgcatcg gcatcttcgg cagcaacgat gacgaccata ccgatgccgc
      301 agttaaaggt ccggtacatt tcttgggttt ccacattgcc cgccttttga agccattgga
      361 agagcttggg caattcccac gattcggcat cgatttgggc aaccgtgttt ttaggcaaca
      421 cgcgcggcac gttttcggta atgccgccgc cggtaatgtg cgccatacct ttaatggtaa
      481 atttttccaa agcggcaaga atcggtttca catacagacg tgtcggcgca ataacggctt
      541 cacgtaaagt tttgccatta tcaaactcgg catccagatc gggattgtcg cgttcgatga
      601 ttttacggat aagggaatag ccgtttgaat gtgcgccgtt ggaagccaaa cccaatacca
      661 catcgcccgc gccgacgctg aggccggtaa tgacattctc tttttccacc acgccgacgg
      721 caaaacccgc caaatcgtat tcaccaacgg gatacatacc cggcatttcg gcagtttccc
      781 cgccaatcag ggcgcagccg gattcttcgc aaccttgggc aatgcctttg atgacatcgg
      841 tcgcacgcgg aacatccaat ttaccgcagg caaaataatc caagaaaaac aatggttcag
      901 ctccttgaac caaaatatcg ttgacactca ttgcaacaag gtcgatgccc accgtatcgt
      961 gtttgtccca atcgaaggca agtttgagct tggtacccac gccgtccgta ccgctgacca
     1021 atacggggtt ttgatatttt ttgccgattt cgaccaatgc gccaaaaccg cccaaatccc
     1081 ccaatacttc cggacgcatg gtgcgtttgg caaacggttt gattttttcg accagttggt
     1141 cgcctgcgtc gataccgacg cccgcatcgc ggtaactcaa tgaagtactc atcgtttttc
     1201 cttggtaaat ggggattgga cggtaaaata acggggcgta ttctacctta tttcgcgttt
     1261 gcaggttcag atttttagac aatattgtaa acaatccgcc atatgcccgc gcgtgtcggg
     1321 tttggcgggg ccgtccgcag gattaacggg cagaaacccg cctgcccttc ccctcaattt
     1381 cttatatatc gcgttccatc aaaagacgca ttgcttttct taaccattcc ttttggcaga
     1441 cgggcggaag ggtttttttg atgccatcat caaaatcaat attttcttct ttccggttaa
     1501 aaccccgaca ttaggggcgg cgaatccgat tgcgggcgga agcacccgct tccgattcgg
     1561 tgcggaacaa atggcggtgc tttatgtgcc gttttgtgtg ttgaaacaca taggcagata
     1621 aaaagccgcc cgctgaaaag cagacagctt atattttgtg ccaccgaatt gtcccaataa
     1681 tcattaacaa tatgatttat atatttttat tggtgcggac ggagcgcatc tacgcttatt
     1741 ctacgtccgc acttctttcg tctttttaaa tctatttatc cctacatatc aaagcattaa
     1801 atttaatata gccctatttt gccccgcttt atatctgttt tgctctatta tatttgcacc
     1861 cgaattgcac ccgaataaat atggcaacca tcactcaacg taacggcaaa tggcgcgtac
     1921 aaatccgtat gaaaggcgtt tcccgttccg ccacatttga gcgggcatca gacgcgaagg
     1981 cgtgggcggc gcgaatagag tcgcaaatta tggacggcat ccaaggcaat gccccgcgaa
     2041 atactatctt tgccgacctt atccggcggt atttatccga ggttacacca tcaaagcggg
     2101 gcgcacggga agaatcatac cgcattggac gcgcactaaa aacacctttg gcaaaggtgc
     2161 ggcttgccga cctgcgccct caagacttcg ccgattggcg agatcaacgg ttgcaggagg
     2221 tatcccccac cagcgtcgga cgcgaattaa ccactttatc cgccgtctgc gaacacgcca
     2281 tgaaagagtg gggacttctt cgcgaaaacc ctgtacgcaa aatcagcaag ccgaaaaaaa
     2341 gccgggcaag gacaagacgg ccaaccgagc aggaaattgc cgatatttgc gccgccctcc
     2401 tataccggcc taatgagaaa ccgaaaatgg cggtgcaacg ggttgccgtt gccgtcctat
     2461 ttgccatcga aaccgccatg cgggcaggcg aaatctgcgg cctaaaatgg gcagatgtga
     2521 atatgcggcg tcgtattgcc cacctcccaa taactaaaaa cggcgacagc cgtgacgtgc
     2581 cattatcctt gcgagccgcc gaactaatag agcaactgcg cggaattgat gacacatggg
     2641 tgtttagcct ggatgccaaa agccttgatg tattattcag gcgagcgcgg gataattgcg
     2701 gcatacaagg cctgcatttt cacgacaccc gccgcgaagc cctcacgcgc ctatcaaaaa
     2761 aagtgcctgt ggaggtcttg gcaaaaatca gcgggcatcg ggatttaagg attttactca
     2821 atgtttacta ccgccccgac atggcggata ttgcaaaaat gttggattaa aatattttta
     2881 tttaaaaaca tagggcacac tgcgtattga aaataaagag gaaaattaat tatcttcggt
     2941 ttcaaaggtt cattaaaaaa gaatctataa aaaatgccgt ccgaaagccc ttcggacggc
     3001 atttttattg aaaggctctc ttcaaccgct ttacacgaag gcggtttttt tgtatagtcc
     3061 ggtccgcggg gtgcggaatc ttgaaaatag tcaggcaatg ccgtatattc cgacgcaagg
     3121 atttattctc aacatcagct taaggggatg acaatggggc atatttattc ggaaagccgt
     3181 gatttccgcc tttcggacgg ctgggacggc aatgactgac aacctaatac agatagcaac
     3241 gccgatattg actgttatcg gcgtttttgt tgccgcttac ggcatcatga ggaatacgga
     3301 aaacgccaaa aagcgcgcca ccatcgacat gatcatggcc gaacgtaaca atgccgccct
     3361 tcaagaagcc ataaccatag taaacgggct ggcaaaaaca gacggatgca tactcgccac
     3421 ctatacatca gataccccgg acaagaagaa agaccgtgaa gccatactga cagttttaaa
     3481 ccagcgcgaa tttgtctgtg cgggcgtatt gggcggggcg ctgcacgaga aaatgtataa
     3541 agatttcgaa tactccatgc tgttacgcga ctgggacaac ctaagcagct tcatttttga
     3601 aatacgccgt atcaggagcg cgccgacggc ctttcaagaa tttgaagccg tagcccggaa
     3661 atggaagaaa aagcctctga aaaccaaata gcttaatagc ttgacgtacg ccgcaacata
     3721 ggccgtccga aagcccttcg gacggcattt tctcagacaa atttaaagtc ccgtttcatc
     3781 tgcttcaaga gcttcgccaa gtcattttta tggatgaagt cgcctcctgt cgaattgatg
     3841 atgacggtgc tgccgcctcc ctgctgatcc gccatttcac ggatggtttg cgcgtgttcg
     3901 gcaggcagga ccatctcgtt ttcgtgcagt tgggtcagcg ggttgatgcc tgccggaatg
     3961 tcccagccgc ctgccgccga gggaatgcgc gtcgtggttg tggtcgtttg agaaccgccg
     4021 ccgccgccca tcagtcccat tacagccgac atcatcgccg ccatagccgc aacggcaaga
     4081 atcgggccga catatgggat atgcgcctgg gaagccgccg cgcctgaagc cgcttgaaca
     4141 gcattactac tgacaaccgc cgttgtctcg gttgccttag ttgccgccgt cttggctgcc
     4201 gccgctgtct ccagcgtttc cttagttccg aaaatcatct tgtaaatggc cgattcctca
     4261 accatgcgcc gcatcaagcc cgtcaaaggt ttcgtaacca tttcctgaat aaaggtttgc
     4321 cccatacttt tgaagaaacc gttcatcgcc tgcctgaagt tctgcgccct cgccagcatg
     4381 gcggtgaacg cctgccccat ctgctgctgt gcttcctgcc aaacgttcct gccgccgtct
     4441 tgcagcattt ccatgacgtt gggcgcgtct ttgcggcgtt ggttttcgcg tttgccctcg
     4501 ttcttcgcct gcgtccgttc gtgcccccgc cccaattcgc ccatttgggc cttgagcttg
     4561 ccgattgccg cctgactgta tgccgggtct tgttcggcaa gtgcgatccg ttcctgcaat
     4621 gcgtcatagg caatccggta acgtcggttt tcaaactcga tttccaagtc caggcgttcg
     4681 agttgcgaaa tacgtccgtc tgccagggct tggtctgccg cgtctttctc catctccagc
     4741 ttgtgcttgt ccaatttctc ccattcggcc gcctgattca ttttggcttc ggttgactgc
     4801 ttcgataatt ggtcttcaag cgtcaggatt ttttcgcgga ttttcacgcc cgttttgccg
     4861 ttggcgtcca ccgttgccag cttcgcccgc caatattccg cctcacgcgc caaatcccat
     4921 tcttggtgtg cgagcgtttc gcgctgcatt tcgcggtgtg cgagttttcg ggctttgatt
     4981 tccccttccc aagcctgcat cgggtctttg gccgcgcccg gaccgcccga acgggatttg
     5041 ccccctttgc cgccgccctg atttgcgccc ggctttttcc gggcggtttt ggcgtgtcct
     5101 ccgccgccgc gtcccttgag ggcatcggct tcgtgaatat cggcggcccg ttcgcggacg
     5161 gcatttgcca ttgcgccggc gcggtctttc gtcaagctgt catgaatacg ctcgccaagc
     5221 ccgccgtcgt ccatccgccc catccgaacc ttgttcagct ttccaatgcc cgatatcccg
     5281 gccatcgacg cggctttgtt ggcaaagtca atcatgctgt taatcatgcc taccgctttg
     5341 tttatcatcc gttcgatcgc ggaaataaac acgttgccta tggccttgcc gagattggcg
     5401 aagaattgcg gcatatcgtt tgcggcttct ttaatcagca tccagccggt tgcgaatatg
     5461 ttgatataaa cgttcacata cgcgccgatt gcgcttgaga tgacgctcat cacgcgcccg
     5521 aacaacgacg gccagccgcc cgcgccctcg ttcagccggc ctgtcagccc gtcaaaccac
     5581 gatttgacgg ttcccaccgc ttccccgacg gtttccgtga tgatttgcca aaccgcccga
     5641 atcacatcgg aaagattcga ccagccgccg ccgaaaacat cgatttcatc gccgaatttc
     5701 gcaatcagcc cgacaaccgt gccgattgcg acggcaataa tcccgaacgg gtttgccagc
     5761 agtgcaagat tcaaagccag tgtcggtgca acggcggcgg caacggcaac ggcaaaacct
     5821 gcgacgatag ggacgaccaa attaaggtta tcggcaatca gtttaataac ggcggcaatc
     5881 cccgacattg tgccgctgtc gttcagcagt tttgaaacca tgctttgcca gttgttcgag
     5941 aacaccgtca aagcctgccc catcgtcacg ggcattttgg ccgcctgctc cccgaacttc
     6001 tccgacgcgc cggaaatggc tttgaaaatc acatccgccg tcaatttccc ttcgctgccc
     6061 agctttttga tttcagcgcg ggatttgccc atatattccg caatggtatc cagcaaaata
     6121 ggtgcggctt cggaaatgga tttaaattca tcgccctgca atacgccgct gcccaaagcc
     6181 tgcgataact ggaacagcgc ggcggcctgt tgtcgcgcgc caacgccgcc gatggtcatc
     6241 gcgttgttgg ttgcctcggt gaattttaaa atttcctgct gcgtgtagcc gtagtctttc
     6301 aacgcgcggc ttgtggaaac gtacaggttt gccgttgatt caagcgatgc acaggtacgg
     6361 tttgccgtat cgagaagctg ctgctgcacg gccagatact ccgtttcaga cgacgtgact
     6421 tgtcggactt ggctgtttat cgactgcatc gcatcggcgg tatcaagcac cgatttggcg
     6481 aagtctgcgg ttgctaatcc tgccagcagt ttggtcagcc ccgataagcc tgagccgatt
     6541 ttacccactt cggccctaac cttttcagac gcctgttgcg cctgattgcc cgccgcttca
     6601 aatcccgctt tcacatcggc gtgcaaattg cgcatggtct cgtgcatact gtcgattgca
     6661 gctttaaagc cctttgcggc attatcgccg ctgctgcgca tcttttccgc cgccttatca
     6721 gcatcggcgg cggccgaatc aaaaccctgc ttcgtatggt tttctaccgt tatcctgact
     6781 ttggcttcca aatcgctcat ttacagcccc aaataaaaaa gccgtctttt cagacggcat
     6841 aacgtttcaa catttcccga taattcgcca attcccgtcc ggcaaactca aaggccttca
     6901 aatcggcacg atcgcccgca tttcggcgcc cgtccggccg gcaggcggtt tgtttttcac
     6961 aaaatccttc ggcgttcatc tctgcaccct gtcaaaataa aaggcttttt tgcaaccgct
     7021 ttacacgaag gcggtttgac gcgcagggaa tacaaatgcc gcctgaacct ttcagagtgc
     7081 gaagcctgcc cgcagggtgc ggcatcggtt gttgtccgga tcggtacggc attttcattt
     7141 ccgcccatta ttggtgcgct taataatttt gtataacgcc cccttcgcgc aaacaaaacg
     7201 gcaaaaattt gccgcgtccg cccgatatgt aaaaaatcgg ggggaatgcc gacgcttccc
     7261 ccgatgcggc cgccaacccg gaaccgacgg ttcggttgca aacttccggt tggcaactca
     7321 atccgcgccc aacgcccgta ccagcgcgcc gtgccgcgcc ctgcagtcct tgtacaagcc
     7381 gatgacttgc aacgaccacg gcagcacgtc cgcgcccgtg ttcccttcaa ggtgcggcag
     7441 tttcgggcag ggccgcacca aatcggcggg cggcttaacc gccgtcgtca acggcggcgt
     7501 tgagttcgcg cacgccgtca gcatcaaaac aggcattgcg gtacacaggt ttttcaatga
     7561 ttttaagcgt ttgcacatag cgcacccttt ctttttcctc gcgcgccgcc ttttgcgcct
     7621 gatacgcggc ggacgatttg cgggcgtgtt cggcgtgttc gaccgcggcg gctttgaggc
     7681 gttccgaaac ctccagcata gccgcaccgt atccgcgccg gtattgcgcg gcgcggtcgt
     7741 attgccacgc gccggcaacg aacacggcgc aaaccgacac cgcaatcagc ttcgggtttt
     7801 taatcaggcc gttcataagt tttcaacatc gctttgtagt ttttgatttc gctttcggca
     7861 aactcaaaag ctttaaaatc ggcgtttccg cccgcctctt tgcttttggc ttcccattcg
     7921 gcgatacgcg ctttcagaaa ctcggtagga ttcatcatta cgcctctccc gcgccttgcg
     7981 cgccggtggc ggcggcggca gtcaaccggt aacgcccttc ggcgggggac gacggcacag
     8041 gtttgccgcc aatcagcttg gacggccaga agtagccgtc aatgtccgca gggtcaaacg
     8101 ggatgatgga taccatattg ccctgattgc cgcccaagcc caagattctg ccttccgcgt
     8161 ctttgccgac aacgaagaac acgtgtccgc cgccccggcg cggtttgacc gcgatgcagc
     8221 cgtatgcggg ggcttcgagt ttcgtcaaac ccgacattga ccaggctttg gcgcgatacc
     8281 agtccctgat gaccgcgcgt cccgctttgc ccaggcagtg tccgacaaac aggccgcacc
     8341 acggggtatc tgtttctttc caccacgctt tctgctcctg attaaaattt cccatttctt
     8401 gcagcatcgc taaaagcaca gggtttgact tgccattcac tttttcgtga gcgccaaggt
     8461 atttacgggc ttcttcaatc catttcaatt ctttcatttc ttttccttcc gataaaagac
     8521 gagatgtaaa ctttcggttt acaccccggc caagcctttg attgcaggtt tccgcaacac
     8581 cgatacatcc ggtttcacac gttccgtttc catctccact cctgttacac gccgccgctt
     8641 ggcaaaatcc gtttcaggcg gcataatgta tcgacaacgt atctacaaca cgaaagcaaa
     8701 accatgttgc gactgcaaaa atggggcaac agcgcggcag tccgtctgcc cgcccgcctc
     8761 ctgaccgccc tcaacgccaa aatcggcgac gcgctggaaa cagaaatccg caacggcgag
     8821 ctgctcatcc gccccgtcaa acgctaccgc ctgtccgacc ttttggcgga aacggaagcc
     8881 gccccgccgc gcgcggaagg ctgggaagaa atgcccgatg tcggacagga gggcgtttga
     8941 tgtatattcc cgagcgaggc gacattttcc atcttgcctt cgaccccgcc gccggtacgg
     9001 aaatgaaagg cggccattac gccatagccc tgtcgcccag agcttacaac cgcgcaaccg
     9061 gcttggttta cgcctgcccg atttcacaag gcagggcggc ggcggcaaga agcggcggta
     9121 tgatttccac actgctcggc gcgggaacgg caacacaagg caatgtccac tgccaccgga
     9181 tgaaggcatt ggattggaaa atccgccgcg ccgccttccg cgaaaccgtc cccgattacg
     9241 tgattgaaga cgtattggca cgcatcggcg cagtactgtt cgactgactt cagagtgcga
     9301 agcctgtccg cagggggaag catccgtttc catacccgcc cctatttcaa agcattcaaa
     9361 atatccgccg gcttccaaat caacgccgtc aaaggcgcgg cgcccaaaac cgcataaaac
     9421 atacggcgca acaccgcata ctcggcaaac agcttcaaca tcttgcgaaa ctccctgttt
     9481 atgctaaact tcaaacagat ttctccttac ctcggataag gttaaattca gaaacgccgc
     9541 agggattccg cccctcgcgg cgtttcgccg ttttcaaacc tgccgcgtaa tgtcgtgcgt
     9601 cagccggacg cgcccggaac acagcgtctt cacgcgccgc gataccgctc gaaatcaatc
     9661 ttttccatcc gaagaccctc cgtctttcac acccgtaagc ttttccgcca agccgcccag
     9721 ataaccgcgc aacaccttgg gcgcgagcgc gcgcaccgta tcgagcgcgt gccccgtcat
     9781 catccccgcg aacacccccg ccgccgcgca cgtccacgcc tgatccagcg gcacgaaccg
     9841 ctccgccgcg gccgccgccg cgattgcaga aataaaagcc tccagcacag cctgcacggg
     9901 gcggccgcgt tcctgaaggc ttgcccacac cgcgcccagc acgccgccgg ccgccgcaaa
     9961 cagcaaaccg aactgcaaga accccattca cgacccctca tccgccaaac cccgcgactt
    10021 ttgcgccgaa aacaaaaacc tcaacgcatt attccccgcc agcgcgcaga aaaacgccaa
    10081 cagcggcgga acgaccatac ccgtgttcaa aggcgggtac gccccccaaa acgccaccga
    10141 aaccaaaaac cacacaaagc ccgaaaacaa aagcaggtag gccgaagcca cacaagccct
    10201 cacgccccgc gcaaacagca atgccgtctg aagcagcccc gccgccgcca gcacccccac
    10261 cgtccacgcc tccggaatat cctggaattt gtaataaatc cgccacgcgt aaatctcgtc
    10321 gggcgcaagc gcgaacaccg ccgcgtaacc cgacaagccc aaaccgctga ccgcctccag
    10381 cacccgcgtc gccgtgccga acaaccacgc ctgaaacccg gcgggcaaaa accgccgttc
    10441 cgtcaaaaac ctcaacatac ccgtcacaca ccccccggca aaacaccgcg aagccgaagc
    10501 cccgcagtgt ccgacgccgt ctgaaaactt attccgcgta aaccggttgc gcgtctttgg
    10561 ggatgcccgc cgcaccttcc aacgccttgc ggtacaccca accggggtcg ggcgcggtta
    10621 tgtccgtttc ctgaaaatcc aatgtgcgcg ccaacaggta gcgtttgccc gatttaaacg
    10681 cattttccga cacatagccg ttcaacgtcg ccgtaacata accgttgcgg taatccgcgc
    10741 cgatatactc gacgacgtgg taggccgcac tcgcgccggt cataccgtcc tcgactgcaa
    10801 aattcaaggc aacaatcttt ttatcttcaa tcatttcaaa tctccaaaag cccgctaggg
    10861 gcgggaacga aaatgccgtc cgaggtcttc agagggcatt gcccggacaa aacaggaaaa
    10921 acccaaacgg cggcatacgg gggcgtcagc caacctgcca aacctcgttt tcgcgcgatt
    10981 ccggaatcag caccctgata ttgcgcagct cgatatactg ccaccagtgt tccacaaaaa
    11041 acagcaggtt ggatttcgga acggcagtat taaactcctc ctgaaacaga gaatactgtt
    11101 ggggcgcatg accccggtat ttcttaacca ctacaaactt atccgacctg tcgaacgcaa
    11161 gcatcgtact ccagtcggca ttggtaaaag gccggtactc gaacgatatg ccgtagatat
    11221 cgtccggcaa ctgaagcagc ccgccgtaag cattccaatg tttatacatc tgattttggt
    11281 aatcgtgtac acgccggtcg ccgcccaaat aaacctgctt cttactgtcg taccggaaac
    11341 tttccgcaaa ggtgcgccaa accatcctcc cgagcagctg cttgtattcg tagtcggatt
    11401 gcgccaaata cgaaaccgac acgaacggcg aaaacaccga ttcgtgggaa gcgagcgaca
    11461 acttcaggct gacgggtttg cctgcgggga tgacttgaat ctggtactcg aggcgggttc
    11521 tgtaccttga gccggattgt tcggtataat ccccgccccc ccttgcccgg gggggcaggt
    11581 cttcgtacct cccgaaagtt cggccgcgcc aaatataatt ggtatagttc tcggtttccc
    11641 tgacttccct cggcgcaagc acacccccgt tcaacagcag ctttgcctgc accctgttgt
    11701 tgccgaacgt ttccgaaacc acataaaaat tcggaatcaa aaccctcggc agcgggtctt
    11761 tatccaaatc caacacccaa acgttaccct ccgtccaacg cagcctgtgc gccctcaaca
    11821 cgtcgccctc gacggacgaa gccctgaccg tgccgttgaa ataacccgaa tcggcctcaa
    11881 tccgtccgcg tattaccgca ttgtcggccg tcaggatgcc gtctgaagac accgtaaaat
    11941 tacccccgcc gatattcagg ctgccgcccg tgatgcttcc gagattggaa gataccgcgc
    12001 tcaaatcggc aacctgaatc ttatcggcag tcaccgcgtt tgccccgatt ttatcggcgg
    12061 taacggcggc agccccgatt tcccgcgccg taacgcttcc ggctttcagg cggtttgcgt
    12121 ccagcgtgtc cgccgcgatt ttgccgccgt ggatatcccc ggcgttcaac ttatcgacaa
    12181 ccgccttgcc gttgaccacc aattcgccgt tgatgcccac gcggtttttc tgcgtgtcca
    12241 ccacgaacgg gaacacatcc gccttcccgg ccgcaccgat gccgaagcgg tcggcattca
    12301 cgataaactt gctttcgggc gtgccgtttt tcggcgtagt cgccaagccg aagcccgcca
    12361 ctttgccgtt tgcatcaacc ttgaccgtgt attgcgcctc caagccgttg atgcttcggg
    12421 cgtgtgtctg aaccgccgcc gcattgccgt ccgccgtcga tcggactgcc tcgacgcgct
    12481 cgttgacgct gcccgcgccg ttgccgtcaa tcaggttgat tttgtcgcgc aaagccttgt
    12541 tcagactgct ttccgacagg tcggttgccg atacgtcgta aacggtaaac gatatgctgc
    12601 cgctgacttt caggttatct ttgccgaaac tgtcgtaacc cgccgccctc aagtaatagg
    12661 tctttccttt ctccagcggt ttgccgccgc atttggcgat ggttataaac gtttccgcgc
    12721 cgtcataggc tttgtttgcg tccgcggccg ggcaggctgc gttttcgcaa acccaaacga
    12781 taatccctgc aaagtcttct tcgtcaggtt tttggcaggt aaaaaacgcc tggcgcaggc
    12841 cgctgtcgat agagatgccc tgcaatgcct gcaattgcgg attttgcgcc gcgatttgcg
    12901 cccaattgcc cgttttaccg gtaacggcac gcccgcgaac cttgaagaca ataccgcgta
    12961 cctggccgcc gtccgccttc atatccgcct gagtgtaggt gtagctgttg tcaacgatgc
    13021 cgtcaaccgc acgcagacgg cgtcggctgc cgcctgcgta gatttccacg tcgtaggtat
    13081 ccgcgccgtc caatttatcc caagcgatga cggcttcttt gccgtatgcc cacgatgatg
    13141 tcaggcgcag gttttggatt tgaccgagcg gcgcgccttc gatggtgtag gaatacgccg
    13201 gcacttccgc caaatcctga atgccgccgc cgaaaacgtt gtacgaaacc agtttgaccc
    13261 aaaccgtgcg gccaatccag ttgcgcggaa cggcgtattt gaacaatgct tcgtcgatgc
    13321 gcgcaaaccg gctgcccgcc gcgtgtgcgt tgacggcgga gccgtacgcg ccgcgcgtca
    13381 ggttgcccaa tgtgtagcgt cccacgccct tcagttcggc gtcggcgtat gccagaaatt
    13441 cgccgtcgac gtagcacaat gtcagcaaat cgcggctgtc ctgctccgtg ccgcccgtca
    13501 gctgccccgc cgaaatttcc acgcccaacg tatttgtgcg gtcgaaaacc gcgccgtcgg
    13561 gcaaatcggc ggtcagcgcg ccgaaacgcg ccttgcggtt gaccgcgccg acgcgggtgt
    13621 agctgtcgcc gtccgccgat acccacactt cggcgccgcc ccacataccg cccccggcgg
    13681 ttgccagcca gatttgcggt tcgccgcccg tcagttgcaa cggcgcttcg aaaattaccg
    13741 gcacatgggc gttgcccggc gaaacgttgt agtcggccga ataacccgaa gacggctgcg
    13801 tcggatattc cgacacggta taaacaccgg ccgggcagtc ttcggccttg acggataaaa
    13861 ccccttctcc gtcttcttcg atttccgtaa tgcggacggg cgttttattc aggccgagcc
    13921 ccgcgtcggt cagggttacg atgtccatcg gctcaagcag gcagtatttc caacccagct
    13981 taaactcata ttcgttgcgc acgtacaggg cgcgttgcag cagttgttgc gccacttttt
    14041 gcgccacctt cgcatcgcaa atgccgtgca tcctgaccgc gtctttcggg cgcaatccgt
    14101 actgctcgat attcgcctgg tctttcgctt ccgcgatggc gacgttgtag tcgttgtccc
    14161 tgtcaaggta ctcgacctga atctgattaa acgcatcggc attggttttg cgcccggcct
    14221 ttacagggtc ttgcgcgccc gaaacgatga aatcgtcatc ggtcaggtcg taggcggcct
    14281 tattgtcggc aacgtacacc gcgccgttcc ccgaatggct gccgtccccg taggggatga
    14341 ttttcaggcg gccttgcgaa aataccgccg cgctgttggt ctgctccagc agttcggaaa
    14401 tattccgttg cgcctccccc tgttccgtgt aggcagggct taggaaaatc ccgacggcgc
    14461 ggcaataatt gctgtaccgg tcggtatcgc cgatgttttg ggacgggaaa ccgcagccgt
    14521 agcgttggtt cgtcagcaaa tccaatacga tttcgcgcgg gtttgcatcg gggatgttgc
    14581 cggaatagcc cgatttcccg atgacctcga aattgtgctg atatatttgc gccgatttcg
    14641 tcagttcgta gttcgggctg cacaaataag ccgtgcccga ataattcaag gcttggcctt
    14701 ggtgcttcgc ctgttgcagg tgcgtccaca acggctgctc gtcgccgccg cgcataagcg
    14761 tcaggcgcaa ctgtgccggc gaatcgaatt tctccttgtc gcgccaaacc cgccccacgc
    14821 cctgaatctc gccctcgcac aaagcaagca tgacggcggc ttcgtaggta taggaaatac
    14881 cgacctgttt cacaccgccg ccgcccttgc cgccctgacg cgtcgtagtt ttatgctcga
    14941 tggtgacgaa atcgccgtac caaatcaaat tgcccgccac gcgcgtcctg ccgtagatga
    15001 cgggcagggt cagcccctgt gatgaccgct gaacctgtaa cgataaaatc cgctcttcgg
    15061 cagatgtaac ggttgacgat ttaccaccca taaaaccacc tcgaaacttg caaaaattat
    15121 caaaatcgat ataatttact tatatcaaaa cagatataaa atatgaaacc gttaaatttt
    15181 tcaggcggtt cattggattg cctgcggcaa ttccccgaaa acgccaaaca ggcggcaggt
    15241 tatcaacttc aagaaaagcc ggaaaaccgc gggcgccgat tcggaatcgg cggaaaaacg
    15301 ttacaacaaa ttgattcggg aaaagaaata atggaaagcc aaactttcgc ctccgtattt
    15361 gacgcactgt gcgacacgcc tgccgaagcc gccaatatgc ggttgcgcgc cggcctgatg
    15421 atgcacatcg ccgataccgt ccgcgaaaac ggttggacgc aaaaacaggc cgcagaacat
    15481 tgcggcctga cccggccgcg catcaacgac ctgctgaacg ggaaaatcga caaattttca
    15541 ttggatgcgc tcgtaaacat caatgccgga ctcgggcagt gcatttcctt atccttcgcc
    15601 cccgcgtaaa gcccgtcctt tcagacggcc gcaaaatcaa aatgaaaatg taaaaaaccg
    15661 aaccccgcgc ccgataagtt cggcttggcc gatgtcgtcc gacaccacgc cgcgcccgat
    15721 gtagctgtga ataaccttgc cgccgcccgc caatatgccg ccgtgcgaaa acgaccgccc
    15781 gaaacgccat actgcaatgt cgcccgcctg cggcgattcc gtttcacggc aaaattgcgt
    15841 gacgaacccc aaataacgct cgcaatcgcg gtgcagatgc caatcttgag ggtaagggcg
    15901 cgggtcgaac ccttcgggaa gcagtccgac cgccccgtag acggcgacca ggagcatcgc
    15961 gcaatcaaca cccgcgccct tgaccattgc atgatgatgg tacggcgtgc caagccacga
    16021 ccgcgcctct tcgacgatcc gcgccctcaa atccgtttcg gacggcatat cacaccaccg
    16081 tatctgccga agggatgaac gggaagccgc gaaaatgcac gatattgtca aacttatcct
    16141 tgcaggtatc gcgccgcttg tcgcagcccg gataaacctt gaacgcatcg cccgcctgcg
    16201 gcgggtaggg caggcgaagg gcaagttcga acgtgttgcc gccgtgtgcc ttgaccgtcc
    16261 tgctcaaacc cgcgttgcgc ccgcccgcaa acttaatcac gccctgcgaa aaccagccgt
    16321 caggctgcgt cagattgtgc ttcagcacgg ttcccgtccg gctgttttcg gttacgcgcc
    16381 cgtttaccgt gaatttctca cggttgacct tgcagccgtc gtcatagagc gtcctcatac
    16441 agcccgcctg ataaatgttg cgcgggctgg aaacgttcaa aagctcgatg tcggatttca
    16501 cgtcaacctt cacggacgac ctgccgcccg atacgtccga cacgcgccct gaaaaaatat
    16561 tcaccgcacc gacggggcgc agtccggcat caaaaaacac acggtctatc ctgacccgcg
    16621 cgccgtccaa tacgccgccc agggcggcct ccgcccattg caggccctca agcctgtaat
    16681 cgggcgcggc tgaaatctgc agggtgttgg aatccacttc caatccgacg gcggtacggg
    16741 ttgcgccgcg cttgataacc agctcgtgcg ccccgtaggc ctgaccgtcc caaacgacgg
    16801 gcatatccgc gccggtatgc cgcagcaccc ggccgcccga aagcgtaatg gtgtacaaat
    16861 ccgccatctg aaactcgtcg ccgccgtgca gcaaatcaat cagttctttt gtcgccgcct
    16921 tcatagtttc acactcgtaa actcaatctt tttggccgcc cacaagctgc ccaaaacgtt
    16981 ttcaaaatcc accgtgtcag acgtaaacct cacgcggaaa taaaagccgc ccgtccatgt
    17041 aatcgggcgg cccggcggtt gcggcgtgtt gaaaaccaaa acgcccttgt cggtaacggt
    17101 gtaatcgcgc ccgtacgcca acgccgtgcc gccgaccttg acggcgggcc gttccttgac
    17161 cgccgacaca ggctcgataa atccgcccat cgaacggaca agctgataac gcgcaacgcc
    17221 ctgcaccgtg tttccgacag gctggtcggt tacggcgttg tcggccgggt cttcgtaaag
    17281 gaaactttca aagctgcctt tgcgggcgtt gaagaatccc gccagttttt ccaactcgtt
    17341 tacggacgcc tttgtccgca atacctcgaa cgacagcgaa aaccgccatt gcgggtaggt
    17401 gtagtaggcg gttcggaact cacggccgct tgccgatttc tgcgtccccg tactccatac
    17461 cgccgttttc ttccgccccc acttcaagcc ggggaactcg gggaaaaccg cattgcccat
    17521 cagatgattc ctttcgcttt gagcagggcg tcaaattcgt cttcggacag ttcgccgccg
    17581 ccaagcatac cgacggcttc ggcttcgtcc gcttcgcttt gcacactgcc cgacgacggc
    17641 ttgacgccca tatacgacgc tgccaagata tgcacgggcg gatgttcgcg ccaatactcg
    17701 ttcaggtgtt tgatacgcgg caaatccaag ttgtcggcga cgtagtccca cgtccagccg
    17761 gtagaggcgc aaacgtgggc aatcaccgcg ccgaaactta atccgccgcc ctggcctccc
    17821 ccgcttgtgc ggcttcctgc tccctgcgtt tcaagcccga aacgtccatc acggcggcaa
    17881 acacttcgtt catgttgccg atatcgatca aatcggccgc ttcttcgcgc gttaaatccg
    17941 ggtaattgcg cttcagggcg gcgtgggcgc aatcgataac ggtagagatt tgtccggcat
    18001 ccgccgcatt gccgtcaaat gagccgatgc gctcctgcaa ctgctccaac gcgcccagcg
    18061 caataggcgg aatcacataa tccgcgccgt tcagttctac ggttacgcct ttaatccgta
    18121 cggtcatttt tatttccttg actttggttg aataaaaaaa gccgcctttt cagacggcat
    18181 ataaacatta ctcctgaatc cacagcgtac cgattttaaa gcccgcctcg tcggttgagg
    18241 cggtaaagtc gatttcgggg acggagaagt cgtcgttttt agtcgagaac aagcccaatt
    18301 tgccgctggt tacgctttcc agttccaaca gggctttttt gcctttgaac tgcgtcaggt
    18361 acctcatctt aaacgtcggc gtgttgcccg tcgccatatt ggtcagctcg attttcttcg
    18421 ccgggggcat cgtttgcgta taggtaaagc tcggataaac ggttttgcct ttatccgagc
    18481 tgttaaaggc gtaaacgccc gattccgaaa ccgtatattg gccttgttcc ggggaggatg
    18541 ctactttgat ataagccgtg ccgtcctggc ccatcacgcc cgcgtcctca gcgaacttgc
    18601 cgccgccggg cgcggctgcc tgaaggtaag aataattgtc gccatcgagg actttgcccg
    18661 tagtttccgc ccaaagcgcc ttcatcgtgc cggttgcata ttccgtaccg aaaaacaggg
    18721 tattgagggt caggccgtta atcaacgcgc ctttcatttt gcccgacacc ttgaccttgc
    18781 cttgcgccac agccaaagga taacggtttt gaccgtagaa ctctttcaat tccgccgaca
    18841 aatcgacgga catttcctgc aagcccataa tccgcacggg cgttgcgttc tgtacacggt
    18901 tgccgtaagc atccgtaatc atttgggcga acacttcgcc gctgccaaac gtcaactgca
    18961 tgacatccct ttcaaaaaaa gccgcattac gcggcgcaaa tcacaatcgg aataatacaa
    19021 accgcctgtt cgccaagcgt cccctcgtcg gtttccaccg taccctcgac gcggcaatac
    19081 tcgatatccg cgccgtccgc cgttaaatcc gtcttgcccg taacggggtg gacggcgttc
    19141 acggcattgc acaccgcgtc aatcagcgga ttcatgacgg gcgcgggcgg ttggcccgcc
    19201 gtctggacgt acaggtaaac atcggcgcgc aaaatccact tggtctcccg ccccgtcaat
    19261 gtcaacgcct gcatatcgcc ctgcgccata aataacgcgg gctgatcgta gcgtttcaca
    19321 tcgttccagt gcaccagctt ccggctcttg gttacgaagc cgtccaatgc gtccagtttc
    19381 gaccaaagcg cggaataaac cgcctcacga ttcatttcaa cacctttcca atcgattttt
    19441 gcaggtcgtc cgcgaacttc ggcgtcaaat cgcgcaatgc cgaacggagg aacgaccgct
    19501 ccggcaactt cacatcacgg gtatgggcgc ggacgtgaac atatcgcggc ggtttcagcg
    19561 gccttccgaa cgcctgcctg acctggcgca gcgaagcctt aacgttgaca ctgcccgcga
    19621 aaccatactc atgcgcgatg ccgtaacgga cgttcgtgtt gacttcgccc gaaacaacat
    19681 tgccggaaac gttcacgcgc tggtgtatgg agcgtctcag gttgtccgtc cttaccctca
    19741 acacccggcc cgataaccgg ttctgcttga cttcgcgctg caggcgcaat gccgacctgc
    19801 ccaccgattt gacgaccgca tcttgaacat cggcggcata tgctttaaaa accgccgcca
    19861 gaataccgcc gccgataaat tccatcttca gcatcacacg ccctttcgtt tgtactcatt
    19921 gagtatcgca aacgccgacg gcggcatacc gccggaatta ccgaacgtag aaaaagcgac
    19981 ggtttcgcct gcaagcgtct tgctccgtac gcccttgccc tcgatttcgt tcacgcgctg
    20041 caccgcgata atcatcaccg cttcgcgtat gtcggcgggc atgcgctcat aaccggcgcg
    20101 gtacgatacc tcgacatttt tcagcccccg cgcgaaacct tcggggcgca tcagcagcca
    20161 gttgtcaaac tcccagccgc cggcatccgc gccgttgatt ttgacggacg acaccgacag
    20221 gacgggataa ttgtccaaca cgatgcggtc tttgccgttg ccgtcgtacc gcccgacata
    20281 atcggcggcc aggaggctgc gcccgatata ggcttccgcc gccgccgaca cgccgtcaat
    20341 gacgctgagg aagaaatcgt cccgccggtc atgttcaacg ccgatacgct gcttgaactc
    20401 ctcaagcgat accggggcgg ccatcgttat tcagcctttt cggcttcggc aggttcggca
    20461 gcttcggcag cttcggcagc ttcggcttcg gcttcggcag gttcggcagc ttcggcaggt
    20521 tcggattgtc ctgccggctg cccggtttta ggtttacgcc cgcgcttggc tttttcaggc
    20581 ccttcggcag gctcggaggc aacgttgcca aaaccgaact gatacaggaa ttccgccgcc
    20641 tccgacggca cttccacgat accgttttca tctgccgcgt agctttggct gccgaaggaa
    20701 acgtcggtaa acccttcggg cgcttttaat ttgactgtca tctcaaaatc tccaaaagaa
    20761 aaagccgtct gattttcaga cggcatatcg gcttaaccca cgttggtaat cataccgaag
    20821 gcaggcatga acataccttg cagcacctcg tccgcataga cgccgtattc gtacatacgg
    20881 gtgcgcagcg gccattcgat ttggtaatac tcttggcgcg tacgcacttg cagcagattg
    20941 ccgacgccct gaacgtaggc gggcaggcgg cttgagtaga acaggtaagt accggcaggc
    21001 aggttcgggt gtaccacgat gttcaggtcg tcgcccgtga tcttgttcat atacgaaccg
    21061 acaacgacac cggcgcggat gttcgccgta ttgttcacgt caacgttcaa cttaatcaac
    21121 ggcgcgccgc tgtttccgat aatcagcttg gtcagcgccg ccaaatcgcg ggcgttgacg
    21181 tagacggtat cgggggacag gcggtatcgg gtaaagaaat gcgcgaacgc ctcttcaaat
    21241 tcatacacgc cgcccgcgtt gtcggaagtc aggccgccgc ctttgttgtc cgaccagaac
    21301 gcgcccgaat caggcagggc gatttgggtc aacaggccgt caaattccaa aatagaagtg
    21361 gaattgtctt cggacggcag ggaagcggcg gtctgggtac cttcggcatc agccgaaatt
    21421 tccactttgg cggcagtggt aaccgcgccc agtttttcag aaccggccgc accccagtac
    21481 caagcatagg caaccgcgcc gcgaacagcc ggaatcacgg cggttacttt tttgcctgcc
    21541 tcaataccgg aaacggaagc cgccgcagat tttcgggcgg aaccgccgcc gaacgtatcg
    21601 gttgtaccat ccgcgtttcg gcgtgtgatt ttggcaggga cttgggcagt tttaatgttc
    21661 gggctttgac cggttgcacc gttgtttgcg cctgccacgt cccaatacgc ctgcaagccc
    21721 aaagccacac agattacaga caaggtgctg acgctgattt tccccgccgc gtcggcggaa
    21781 acgacggcgg tcggtgtagg tgtaacgcct gctttcaggc tggtgttgcc gcccagcaaa
    21841 atcatttctt cggcaaccat agtagcctga agggtttggg cgaccgccaa cgctttcacg
    21901 tcctcgaaac cgcgcgcggc gtaatccgct tcaaaggtta cttggttttc caagccgatg
    21961 gcgcggaatt gcgcgttgcg ttccaccgtt tcgtgattga tgacgccgcc gcgcctgcct
    22021 tcgccgatac cggcgcgttg gttgccgacg ttgatattcg tgacggcttt ccagttcgag
    22081 ccgatggtgc ggccgccgcc cacgcgcggg atacggttgc gcaacggggt caataccggg
    22141 tagagttttt gagacggcgc ggaaaggtca taggtttgca ggccggcggt aaagccggcc
    22201 ggctgggtaa aacctttgtc aggcggcccg ccgcccgctt gtgccgactt catcagttca
    22261 atcgtttctt gtgtgagttg gttcacgttc atttatcgct cctgataata aaaaaaccgc
    22321 ctgtaagcgg tgttacagac ggcctgtttg ttttgcctta atgagtgtcg ccacgtcatc
    22381 cagcgtgccg tcattcttta caatcggctg aaaacctttt aacgggtctt cgccgttatc
    22441 ttctgcctta ctgatagctt tcgtactgcc tttcggcggt actgcctgtt tcttcaggct
    22501 ttcgatttcc gcctgcgctt tggcaagggc gtcattcgat tttttcagcg cgtcttgtgc
    22561 ctttgccagt tcgtcagccg attccgcttt ggcaagatcg cctgatttgc cggctttggc
    22621 cgccaaaccg tcggccggct tatcggcttc gcttgccgcc aacgctttca gcgattcggc
    22681 aaggccggcc gccgattctt tgatttgcgc ggtaacggct tcatcgatgt tgtcgtagga
    22741 tgcgtcctca accagccatt tcagcgacat caatacatca gccggtgatt tgacttggta
    22801 cattgatttg gcggccgtcc cgcctttcgg cttgtcggct ttagccaata ccgctttcaa
    22861 ggcggcgatt tcagattcgg acaaattcac gcttgccgat tttccggctt cgtccttttt
    22921 gccggcgtcc ttatcgcctt tatcttcggt tttaggccct ttatcgccgt ctttaggatt
    22981 ttcatcttcc tcttcggttg gtttgtcaga cggcttgccg tctttatccg ccgcccctgc
    23041 ctcatctttc ggtttgtccg ccttaaagca ggtaaacacc gcgtcaggat tggcagggcg
    23101 gtcgacaagg ctgatttccg tcagcttcaa gcccgtgatt tgcgacttgt tcaaatcatc
    23161 gcgggcggta acgctgccgc cgatggaaaa gcctttgtaa acgcctgtct tgactttcgt
    23221 caccgcaaca gggtcaacga tatgcgcccc gaaaaatgtg cgcccgtcgt cttccacgtt
    23281 gatttcaatc gccgttcccg ccgcgtttga gccgtgcatc tcgcgcaccg cgccaaactt
    23341 catataatcg ggaatcgccg ccttcattgc ttccgccgcg acgacttcgc cgtccgaatc
    23401 gaccgcttcg ctcgaggcat agccccaaac tttgaccgtg ccgtcgtcct gcgcctccgt
    23461 cttggcgatt tgcgcgtata acttcgtcat tggtttgctc caaaaaaaag ccgccccgct
    23521 aaggggcggc aaaacacacc cgcctgaccc gaaggaatca agattcaggc atatcccctg
    23581 ccaaaacagg cacaaccgcg caccggcagt tcggatgacc gggtatcgtc aacgcaccgt
    23641 gggagaaagg ctcgtgcaaa ccgattacgc ccatcccgcc gttggcattg cagacatcgg
    23701 acaccttgtc gtcttttgcg gttatccact gcttgccggc aaccagcccc gttccttccc
    23761 agcctatcag gttgcccata ccgtccgcca tcgccgtttc cgttcgggca atggttcggg
    23821 cgcgtgcgtt gccgaaagca tgggattctt tcaggcggcc tgccaattcc tgcacactgt
    23881 cgccgtttcg catggcttcg gctacttggg cgcgtatcat ttcgcgcgtc ccttcggtga
    23941 tttgccactc ggcggcagga tttcggacaa gcccgccgcc cgcccgcttc atgccgacca
    24001 tttcggcggc gcgttcatgc gcccacttga cggcgcggct gcgaatgttc gtaaccatac
    24061 cggcggcagg ttcaggcatt acgcgcaaca aggcggcaac cgccccgtct tccgcaacac
    24121 gcctgattat cggctcgacc acatcggaca atcccgacca atcgccaaaa tccaagccgt
    24181 ttgcaacaac ccctgccgcg cggctcaatt ccgccgccaa atccccggcc tgccaatcga
    24241 cggccgcacc ctcaatcagc gccgcgattt gttcggccaa gccgtcaatg cgtgtcagca
    24301 aataagcctc aataagcgcg gcagattcgt cttcgctcat cgggctttcc gactttccca
    24361 acttttcagc cccttggttc ggcggctctt cgggctttcg gccgtcttgc ttatccggtt
    24421 caggctgccc ctgccccggt aacggctcct tgcccagttc ggcgcggatt tcgtcggcgg
    24481 tcaagatgcc tgcgtttttg tagatggcgt agatttcagc ctgttctttc gggttgagcg
    24541 attcctcccc cttccagaca aactcatacg ccgccatatc catgtaacgg gcaagcacgt
    24601 cgtcaatcag ggcttttacc cagtttttca ggctgcccat gccgtctgaa agcgactgct
    24661 cgcggctcgt ctctgccacg ctgcggttta cctgcgccac gaacggcgta ggctcgacac
    24721 taaacgcaaa gcagacgaca cgcgccagcc attcgtcgta aacgtccttc aacggcggct
    24781 gcttcgtctc gcggaagttt cgggacaact cgcccggcac gaaacgcatt ttgcgccgct
    24841 gcgccgtttc gcccgacagc agcaaatccc agtattcttg aaaccgcctg atgtcgtccg
    24901 ccgaccacgt ttcaggcacg ccgactaaag catcgggcac gctgcccgcc gtgtagtatt
    24961 ccagcgcgtg aacctgccgt tttaaggcaa tattcacggt catgatgatt tgctcgacgg
    25021 gcgaatagcc gtaaaccttg taacttcggt tattccgcga acggtaaatc aactcgtcag
    25081 ccgtgtaatc gaccgccgcc atgccgtgca ggatttgctg atacgccgta tcgggcggta
    25141 acggcatacg ccccgtattg tccaaaacgc gcttaatcgt cgccccgtcc atcacttcga
    25201 gggcgtacaa gccgccgccc agtgttttgc gcgggtagat gcacggcgcg tcaatgacaa
    25261 acaggtcttc cagcaagatg cgcagccagt ccgcccacgt atgttcttta tcgggcgacc
    25321 ggaagaacgc aacggcttca tcgacctttc ggtctttccg ttgcgattcg tcgtcttcgg
    25381 ttgattcgac gtcgcgcttt tggattgtcc acttaaggca ctccatttgg tctttacgcg
    25441 cctcgataac caaacgcaat acatcgtagt tgtcggcaag ggcgcgtaat tgcgcaaagc
    25501 ctaccgcttc acgttcgcgc ggcttggaat gcccgacgtt gtagaacggc tcgtaatcaa
    25561 accgccgccc ctctgcctgc tgcgcggcag gggccggagg ctcgcccgcg tcgaaccacc
    25621 cgtccgcgtt gccggtaaag gcgtaacgga cgccggcggc aacgcgggca ataaagcctt
    25681 gcgataaagg tgtcttttta ctcatttcac agcctcgacc tgcgaccgca ggaaatcaat
    25741 catgcccgtc cgggtatcca gcagctcgcc aaacgcacgg cccaaacagt ctatttggtc
    25801 gtcatgccgg ccgttcggga acatgcgcat ttccgcaatc agcgcgtccg tgtcccatgt
    25861 gccgtcgtcc aacaccatca cattgccgat gttgacctgt gccgcgaacg gtccggcgcg
    25921 tgtaaccttg tcgcccgatt cggggccggc ggatacggaa aaacccgcca actggcgggt
    25981 caggtatagt gtttgggatt tgcctgcctg accggggtct tgcgggatgg atattttcgt
    26041 tttcacgccg tctttttgcg ccgtgttgcg taatatcctg tcccgctcgt ccgcgccgta
    26101 ccggccgcgc acgacgttgg cgatgatata ccgcccgtct tccgtaacgc caagcctgcc
    26161 gcctgccgtg tagtcgccgc cgtttgcggt tgaagccaag tcccacgcgc gtacccatct
    26221 gatattcccg gcaggcaacg ccttgacaaa ttgcaggttg tcaggcttga acgtaccgcc
    26281 gtcaggcggc gcaggttttt gcaaatactg cccggcaaac acatacggcg cggcctgctc
    26341 cattcggcgc agtgtttcaa tatcatgctt ttcaggccac aacgccgtgc cgtcttcctg
    26401 aatggcaggc aggcacaaat gctcccactc ttcgccgttg ccgccgtcaa gcagccagcc
    26461 cgccaagtct ttctcgtgca ggcgttgcat aatcaggata atcggcgtgt cagggctgtt
    26521 cttccgggat tcgaccgtgt tttgaaacca gtcgatgacg ttctgccgcc tgacctcgct
    26581 tcgcgcttca tctgctttgt gcgggtcgtc aatgatgatg cagccgccga atccctcccg
    26641 atgcctgccc gcaccgaaac ctgtaatcgt accgcccgca cctgttgcgt acatcacgcc
    26701 gcctgcggtt gttttccagt gatggccgct ttcgcctgcc agtgccaaat caggaaaaat
    26761 cgcccgatac tcttcgtgtt gcaccaagtt ccgaatctgt acggagttat tgaccgccag
    26821 cgccgccgaa tagctcgcgt ggataaactc gcaatcaggc acgcgcccca tcgcccacgc
    26881 gataaagttc acgaccgcga tttccgtttt cgagtatcgc ggcggaatat tgataatcag
    26941 gcgtttcgtt tcgccgttga aaacacgctc gagcgcgttg cagattggcg catggtgatt
    27001 cgcccgctgc caaacatagc cgcgcctttg ataaaacatc cggcgcgtga acatatacag
    27061 gcttgcagaa cttaaactgc gaattactga tgtttcaaca tcgtcgaatt gccctagtgc
    27121 catttcattc taacttcctt tggagaaacg gccatacagc ccctcttatg attagcgttt
    27181 tatgctaaac cctacgcaac acatcttccg ctatcttgcg atattcctcg gcatcaagcc
    27241 gtacagccgc cctcatgctc ccatcgcccg atttaacgtc aagttccgtc ttatcgccgt
    27301 atttcttcgg cgcaatcttg gaagccgccc actttcgggc atctatctgc aatttcgcct
    27361 ttgaaaccgc cgcgctctct gcttgtgcac tatcggcaat ctcgatgatt tcttcagcga
    27421 aatagtccgc ctgcttttct ctcgcgcgcg cgtattggtg gcgaaattct tcattagctt
    27481 ccaaccaacg gtaaatagtt ttcattggcg gcacaccatc ttcggcacat attgaacgta
    27541 ggcttctgcc atttgcgatt ttttcgcata tcttatcggc catgtctttc gtgtaatctg
    27601 tcggacggcc taatttgcgt ttcgtgccgc tcataaaccc tcctcaaaaa agaaaccgtc
    27661 taactctgac ccctctcaga attagacggc aaacacacac tcgccacata ggaaaaacgg
    27721 aacgccgcta cctgtacagg cagaagctca aattcggaca gcctaaaacg caaaaacccg
    27781 cacattgtta tgtacgggct taaaaattca tatccttcgg gcgtgcgaaa atccccgcaa
    27841 gggtaacgat ttgaattata cacctattgc cggaaaaaac aacaggccgt ctgaagattc
    27901 agacggcatt tgtattccct actgcatcaa accgccgaca ggttgcggat ttcgggcagt
    27961 atcgggcgga ttttttctgc gtgttccgcg tcggcgtgtg cgcttaaggc ttcgagggcg
    28021 tttgcggcgg ctttgaggcg gctgcgcgtt tccgcccaga ccgtccacat cgtaaccgcc
    28081 tgcctgcagc cgagctgctt cagcggcagg gaaacgtctc tgcccatttg gatggcccgc
    28141 gcgccgtagc tgacggcgac ggcgaggtcg tacagggcgt tgccgcttat gggcagggcg
    28201 ggttgcggcg cgggtaacgg ttcgcggtcg aggacttcgc ccgtcaagcc cgtgtgcagg
    28261 gtcagcgcgt ggacgtaggc gacggcttcg ggcagcttcc cggcggggat gtcttcgacg
    28321 gattcgacgt tgaagcgttg gtgtaccata ctgtacgctg aggagtagtc tatgcctttg
    28381 cgtccgacaa gtgcggcaac ggcttggcgc aatccggtac ggtcgtcggc ggtggttttg
    28441 gcggacttga tgccgtctga aaccgccaag aacgtttgaa tcacttgcaa ggcgaatttc
    28501 ggtgaaatcc acatcccgta ggcaataacc agttcgcgac aaacaaaagt gccaagtctt
    28561 tgttttgatt gaatagacgg ttttccgtct ttttcaagtt ctttaattaa atcattggtt
    28621 tgtatatttt gaagccagcg gcgcggtgcg tgtctgtctt ctccgcctga agcgcggtgt
    28681 aaatcattca gactgaataa gccgatttcg gtttgatgga tagaaatatt agagatttga
    28741 actgaattgt tcataatgaa gattcctgta gaagtttacg aaattagccc aaacgggcga
    28801 ccgcgaggtt cgtaagcctt ctacaaacag gctgtgctta ttccctgttg aacaggtatt
    28861 gtattcgcag ccctcgcggt caaaagtaaa cttcggttgc gctatcgaaa caaacaacca
    28921 aacaaggaaa ctttagacat gaaaaaatca cattgacgga gtgattgccg ttgtagaaga
    28981 ggttacgaca cctcgtgagt gggaatataa aacaaaaccc cctgcacatg caaggggttt
    29041 tttactattt ctgttcaatc gaaacttcca ctttattgtc tgtgataacg caatatgccc
    29101 gataatcaat ctgcattcca agcccgtttt tagccgtaaa tccacgggta accaagacat
    29161 tgccattctg atttactccg gttgccgtgt ctaggatgtg cgggtcaaac gttctgggat
    29221 gattcaattg agctttgatt gcctcatcac actgactgat gacggctgaa ctatcaatct
    29281 ttttatcctg ttcagcggtt gatttccgac cggaattaag gtcgtcagta gatacaaaaa
    29341 agcgtttgcc attcctgcaa tctacaaaga aaaccatttg ttttgctttc gggttgctgc
    29401 gagaatctga aatatcaaca gtttctactg catcgcatga atcctcccga gcaatatgtt
    29461 cggctatccg tggcaaataa ccattcattt cattgacagc cttctcgccc cactgcttgt
    29521 acaacttcgg ataagctttc ttatcgtact taaattcccg tacgcctgaa gcaattttcc
    29581 catcaatctt ctgcttttcg gctgccgcct tctcttctgg cgttaattcc tttttcggca
    29641 cagcgcacat agcgacaaac agcaaaacaa aaataccgcc cacaatcagc ttgattttct
    29701 ttgctttttt tgccttagcc gcagaattaa cctgcctttg gttgtattgc tgccgccgct
    29761 cttctgggtt ttgtggttca ttataaaggg cgaccttcac cgcttgcagg aatgcgtcgt
    29821 tcttctccac cagcatgcgg cggccatcaa aaaacacaac ctctatcacg ccgccgtcct
    29881 gcacctcata acctgcaata tcaagcagcg caaccttccc accacgagaa agaacaatac
    29941 aatctttttt taagacggct ttccctttat gaaaatcgcc agccagtatg ttgatatatg
    30001 ccatttcccc atctcccatt aagaagtgtt aatttccaca atcataccgt aaccgaaaag
    30061 aaaatcaaac cttctcaaac agcaaatcaa aatcgtcccc tgccgcctgc cgtatcgccc
    30121 cgtaccacgc ggccaagccc aaatccgtct gcaaatgcag ggccccctcg ccgcgccgcc
    30181 tgatttcagc ctgcaaacgt tcctcgtagg ctgcctgcga cttcgcaccg atgccgaacg
    30241 aaatacggac ggcctcttgt tgcggcgcat ccaccttcgc ccatgcctgc aaagtaagaa
    30301 acatggcatc ttcgccgtat ctcaagccga tttcaggctt atgcgggcaa gcttcctccc
    30361 ccatataacg gccttcgata ctcaaacatc tatttaggct gcgcgtatca cggtaacgac
    30421 gctcaaaagc acgcgccaag tcgttcataa attcaaattc ttgctgattc atagcttgat
    30481 taaccctttt tcatgcaaca aaaccaaagt ccgcattaca ccttccgcga aggccgtctg
    30541 aatttcccct tccgcacaat ccgccaagcg gttctataca tcaaaactcc caaatgattc
    30601 caaactctac cgcagcccgc gcctgcaccg tttcggatat ggcggacctc ggcaggaacg
    30661 ccgaaccgcc cgcaattgcg gcaaacaata caacctatat ccgccacttt ttgcagatgt
    30721 gatttttccg cttcggtttt acttttcatc caactccccc acctccaccg ccaaaccgcc
    30781 gcctgcgacc ggctcgccgg cgtaatcggc ggcaatgcgc cgcacctgcc tgtcgttgtg
    30841 ataggcaacg ccttgaagcg cgtccagcgc aaccttcagg gcgttgtcca aatcgatcac
    30901 cgtcttgttt gcgccgccgt ctttgttcgc tttgggtatc agccgcacat atacggcaac
    30961 cgcgccttcg gacggcatcg cgcccgcccc ttgcgcgata cggcggacgg tttccctata
    31021 cgccgccgcc tccgcgctcc tgaccgccct gttgcgccaa atccgccaat atcggtttgc
    31081 cgatacgggg taaggcagga taaggcgtat cacgcgcatt cccctgccgg ttgcgtccac
    31141 aggccggcgc ccaatatcgg gcaggcggcg tgcgcggcgc ggatgtatcc gtgcagcctt
    31201 gccgccgtca tcgcgccggc tcccgccgcc gcgcgttttt ccgcgcgctt ttgccggact
    31261 gccttgttgc ggcaggtttt gcacgatttg taataataga cccccccttc cttgcgcggg
    31321 acggcattaa accctttctc caacggcttg gtcccgccgc aggccttgca ggtgcgcatt
    31381 tcttccgcca ttttgatttt tccttttgtt tcggttgctt aatatccgaa gccgtcgaag
    31441 tcgtcttcgc ctttgccgtc gcgcttgcgg cgcaaaaccg cgtcggcggc ttccaccaaa
    31501 agccataccg ccaacgtcgc cgccccggca aacgacagcg cggtgatgat gcttaacagg
    31561 gtatccattg ttttatttcc tttcggtcgg ttttatgggt cggggtcgga ttcccgccgc
    31621 ctccgtcatt cccgcgcagg cgggaatccg gaccttggaa caacagcaat attcaaagat
    31681 tatctgaaag tccgggattc cggattcccg ctttcgcggg aatggcggcg gtcgggttgg
    31741 cgggtcaaat gcctccgccc gccgtttcag gcggcatcgg gctttcaggg tgcggggccc
    31801 gccccgcttc gggcggcatt ttttccaaca aatcttcgac acaggcggcc gcccacgcaa
    31861 aattgtcttc gccgtcgcgg atgtaataag cggcggagat gacggcaagc agcgcggcct
    31921 ccgtgccgcc gtcgaacccg caatctttca gaccggcgta cattttttca tagcggcggt
    31981 gtttgagttt cttgaacttc ggcgcgtcgg cgcgttggcg ttccaagtac cgcagggctt
    32041 tttccaagtc ttcgcgcccg cctttttcct tgtgccgcca gatgtatttg aaggcgttgc
    32101 cgaggttgaa gttgaggtat tgcgcaaacc cgacgcactc gtgttcgcgg tttttgtaat
    32161 agcccgggtt ggtattgccg ccttgttgcg gcgcggcttc cgtccggacg gtttcggttt
    32221 gcgtgttttg caccgtcatt ccgtatttcc tttcaaattt gcgttttaac gcgtttttca
    32281 atatcgggta ggctgggata ttcccaaagc gttttacccg ccttcccgct caaatccggc
    32341 gcattgcggg gggatattcc ccaagcaccg ccgtcctttt tgcgactgcc gcccccgatg
    32401 ccgtctgaaa cgccccggcg gcgcagggat tggttcgcgg gtaatgcttc gcccgctctt
    32461 ccgcattccg cgccttcgcg catttc
//
LOCUS       DAWTHD010000010        32292 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWTHD010000010 DAWTHD010000000
VERSION     DAWTHD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.010"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(200..1039)
                     /locus_tag="WM704_000365"
     CDS             complement(200..1039)
                     /locus_tag="WM704_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HFA9339874.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAALAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="WM704_000366"
     tRNA            1324..1399
                     /locus_tag="WM704_000366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="WM704_000367"
     CDS             complement(1464..2657)
                     /locus_tag="WM704_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HFA9339875.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="WM704_000368"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="WM704_000368"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFA9339876.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="WM704_000369"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="WM704_000369"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFA9339877.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSNK"
     gene            5103..5528
                     /locus_tag="WM704_000370"
     CDS             5103..5528
                     /locus_tag="WM704_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HFA9339878.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /locus_tag="WM704_000371"
     CDS             complement(5602..7587)
                     /locus_tag="WM704_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIA DNA topoisomerase subunit B"
                     /protein_id="HFA9339879.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVAELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8178)
                     /locus_tag="WM704_000372"
     CDS             complement(7654..8178)
                     /locus_tag="WM704_000372"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFA9339880.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="WM704_000373"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="WM704_000373"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFA9339881.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHMLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="WM704_000374"
     CDS             complement(9712..10710)
                     /locus_tag="WM704_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFA9339882.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="WM704_000375"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="WM704_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFA9339883.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="WM704_000376"
     CDS             complement(12001..12771)
                     /locus_tag="WM704_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFA9339884.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="WM704_000377"
     CDS             complement(12774..13766)
                     /locus_tag="WM704_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFA9339885.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="WM704_000378"
     CDS             complement(13833..14516)
                     /locus_tag="WM704_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9339886.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKHRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14706..16040)
                     /gene="glmM"
                     /locus_tag="WM704_000379"
     CDS             complement(14706..16040)
                     /gene="glmM"
                     /locus_tag="WM704_000379"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFA9339887.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16170..17024)
                     /gene="folP"
                     /locus_tag="WM704_000380"
     CDS             complement(16170..17024)
                     /gene="folP"
                     /locus_tag="WM704_000380"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFA9339888.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEEWARVEPVLAEAAGWGVPVSLDTR
                     RTVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKY
                     QDVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     LPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKV
                     WEALGVNR"
     gene            complement(17164..18057)
                     /locus_tag="WM704_000381"
     CDS             complement(17164..18057)
                     /locus_tag="WM704_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFA9339889.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18148..20250
                     /locus_tag="WM704_000382"
     CDS             18148..20250
                     /locus_tag="WM704_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFA9339890.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAIGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPRLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20314..21792)
                     /gene="ubiD"
                     /locus_tag="WM704_000383"
     CDS             complement(20314..21792)
                     /gene="ubiD"
                     /locus_tag="WM704_000383"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFA9339891.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21812..22327)
                     /locus_tag="WM704_000384"
     CDS             complement(21812..22327)
                     /locus_tag="WM704_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFA9339892.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22405..22702)
                     /locus_tag="WM704_000385"
                     /pseudo
     CDS             complement(22405..22702)
                     /locus_tag="WM704_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22699..22806
                     /locus_tag="WM704_000386"
                     /pseudo
     CDS             22699..22806
                     /locus_tag="WM704_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(22964..23776)
                     /locus_tag="WM704_000387"
                     /pseudo
     CDS             complement(22964..23776)
                     /locus_tag="WM704_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23976..24194
                     /locus_tag="WM704_000388"
                     /pseudo
     CDS             23976..24194
                     /locus_tag="WM704_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702943.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24506..25171
                     /gene="fabG"
                     /locus_tag="WM704_000389"
     CDS             24506..25171
                     /gene="fabG"
                     /locus_tag="WM704_000389"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFA9339893.1"
                     /translation="MADDGFDIAVHCRSRRDEAEAVAEEIRALGRNARVLQFDVSDRE
                     ACREILTADIEANGAYYGVVLNAGLTRDNAFPAFSDDDWDVVPRTNLDGFYNVLHPLV
                     MPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGIIGAAKALAVEPAKRKITVNCV
                     APGLIDTEIVDENVPVGEILKAVPAARMGLPEEVAHAVHFLMDEKAAYITRQVIAVNG
                     GLC"
     gene            25165..25983
                     /locus_tag="WM704_000390"
                     /pseudo
     CDS             25165..25983
                     /locus_tag="WM704_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1"
                     /note="FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; frameshifted; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            25960..26510
                     /locus_tag="WM704_000391"
                     /pseudo
     CDS             25960..26510
                     /locus_tag="WM704_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706948.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
     gene            26564..26639
                     /locus_tag="WM704_000392"
     tRNA            26564..26639
                     /locus_tag="WM704_000392"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26597..26599,aa:Ile,seq:cat)
     gene            26714..27472
                     /locus_tag="WM704_000393"
     CDS             26714..27472
                     /locus_tag="WM704_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9339894.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27473..28537
                     /locus_tag="WM704_000394"
     CDS             27473..28537
                     /locus_tag="WM704_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9339895.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28771..30108
                     /locus_tag="WM704_000395"
     CDS             28771..30108
                     /locus_tag="WM704_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9339896.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            30175..30279
                     /locus_tag="WM704_000396"
                     /pseudo
     CDS             30175..30279
                     /locus_tag="WM704_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(30283..30882)
                     /locus_tag="WM704_000397"
     CDS             complement(30283..30882)
                     /locus_tag="WM704_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9339897.1"
                     /translation="MWKLYADYFAGRPDAETPPENSVKETLKRVGFGKVSFDDREIRF
                     AEKGMGLSLNGIAQGYITDKVVALLKANGVPAALVDMGEIRGFDTNGRRMWNVGIRNP
                     DDEEGVLANITMKDKAFATSGGYGTVMDKAGRLTHLFDPRTGVSTPRYKSMSVMADDA
                     AVADALSTAFSVMDLPLIRSVAESRRLKVRLAMPDNIVD"
     gene            complement(30915..31295)
                     /locus_tag="WM704_000398"
     CDS             complement(30915..31295)
                     /locus_tag="WM704_000398"
                     /inference="COORDINATES: protein motif:HMM:NF014477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9339898.1"
                     /translation="MGRHFGRRRFLTAAAVAVAGAAVSFLPNPFAAGGEKRNMDKKRD
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLAAIFTR"
     gene            complement(31304..32098)
                     /locus_tag="WM704_000399"
     CDS             complement(31304..32098)
                     /locus_tag="WM704_000399"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFA9339899.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
BASE COUNT         7341 a         9250 c         8450 g         7251 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcaag ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttgttcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacaggg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacatag
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgctgg cgcggcggca cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaagc tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcgacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcagaccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg agacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcgaacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaagtt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 ggctacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt attttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc catgaatgtt cgcgcacgcg
     8101 cttaccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag acatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgactttga taccgcacgt tttgacgcac tggaagaacg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactttcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacat gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtggc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg cgcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt gccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg atttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggt gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gtgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgcccccgc
    14641 cggacgttca gacggcattt aaacaggaaa accgccgctg ttgaaacggt tttgcctgat
    14701 tttgcctaaa tgccgccgat ggcggcggcg atacgctccg ccccgtcgcg cgcccaatcg
    14761 gcctgacgcg cctcgaccat cacgcgcacg acgggttcgg ttcccgaagc gcgcaacacg
    14821 acacgccctt tgccttcgag ttctttttcc acttcggcca acacgtcttt cgaagcctcc
    14881 tgccatttct gacccttttg gatgcgcacg ttaatcatcg tttgcggata cggctgccag
    14941 tcggcgcaaa cggtggcgag gtcttggttc aggatttgca gcgccgccaa gacttgcagc
    15001 gcggaaataa tgccgtcgcc ggtgttatgt ttgtccatgc acaaaatatg gccgctggct
    15061 tcgccgccga tgagccagcc gcgttggttc agctgttcca acacatagcg gtcgccgact
    15121 ttggcgcggc agaaatcgac gccctgctct ttcagggcga tttccatcgc catattggtc
    15181 atgaccgttc cgaccacgcc gccgatgttg ataccttctc gggcgcgggc tttggcgatg
    15241 acgtaaatca ggctgtcgcc gtcgtaaacc tgcctgtttt tatcgaccat catcaggcgg
    15301 tcgccatcgc cgtccaaggc aataccgtaa tcggcttcgt tttgcaacac ggcggcttgg
    15361 agggttttgg tatgggtcgc gccgcatttt tcgttgatgt tgtagccgtt gggttcgttg
    15421 ccgatgctga cgacctgtgc gccgagttcg tggaacactt tgggtgcaac gccgtatcct
    15481 gcaccgttgg cggtatcgat aaccaacttc aaaccccgaa ggtcggaatg gccggggaag
    15541 gtggatttgc aaaactcgat atagcggtcg tccgcaccgc tgatgcggcg ggcgcgtccg
    15601 agacgtgcgg acggttgggt tttcatttct tcatcgattt tggcttcgat ttccaactcg
    15661 acttcatcgg aaagtttcac gccgccttcg gcaaagaatt tgatgccgtt gtcggaatag
    15721 gtgttgtgcg acgcggaaat catcacgccg gcggacaggc gcaatgcgcg ggtcagataa
    15781 gccacaccgg gcgtgggcaa cggcccggtc tgtaccacat tcacacccgc cgccgtaaag
    15841 cccgctacca gcgcggcttc cagcatatag cctgaaatgc gcgtgtcttt gccgatgagg
    15901 acggtcggtt tctgatcggt atcgtgctgc accagcacct gccccgccgc atagccgagt
    15961 ttcaatacaa aatcgggcgt aatcggaaac tgaccgactt cgccgcgcac gccgtccgtg
    16021 ccgaaatatt tttttgccat catttgctcc gtgaatgtga accgttgtcc gagattatac
    16081 agtcagtttg cgccctgctg cctgcaccgt tgatgccgtc tgaagccgtc ccgccctttt
    16141 cagacggcat tcggcgcaaa aaccgccgtt taccggttca cgcccaacgc ttcccacacc
    16201 ttcaacgcgt ccgccgtcgc cttcacatcg tgcacgcgca cgatttgcgc tccgcgtgcc
    16261 acggaagcca aagccgctgc cacgctgccg tgtacgcgcg ccgccgcgtc cgcctcaccg
    16321 gtcagctcac ctatcatgct tttgcgcgac acaccgatca gcagcggcaa accggtttcc
    16381 gccatcaatt cgggcaaatg ccgcatcagt gcgatattgt gttgcaggtt cttgccgaag
    16441 ccgaaacccg ggtcgagcgt aatccgttgc ggcgcgatgc ctgccgcgac acaggtttct
    16501 gaccgtgttt tcagataacg tgccacttcg ccgaccacat cctgatattt cggattgtcc
    16561 tgcatggttt cgggcaagcc ccgcatatgc atcaggcaaa tgcccgtgtc cgcctggcgc
    16621 gccagcaatt cgaccgcacc ttcgtcggtc aacgccgcca catcattgat aatatcgatg
    16681 ccgccgagtg ccaacgcctt ttccataacc accgtgcggc gcgtgtccaa actgacggga
    16741 acaccccacc ccgccgcttc cgccaataca ggctcaaccc gcgcccattc ttcttcttcg
    16801 ggcggaacga aatccgcacc cggccgcgtc gattcgccgc cgatgtcgag aatgtccgca
    16861 ccctctttca aaagccgttc ggcatgcgcc aaagctgttt gggcgttttg cgaatacgcg
    16921 ccgccgtcgg aaaaagaatc gggcgtgaga ttcacgatgc ccatgatttt cggtttgtcc
    16981 aaaccgattt caaaccgtcc tgcccgccaa acgtgtcgtg ccatctgaac tcctcccgaa
    17041 ataaaaaaca gattatatgc cgtctgaaac cgtcttgtgc gcttcggacg gcaccgctat
    17101 tcgggcggca gacggcatgt tgtcccaatg tctgcgccgc ctttgaatct gccggcatgc
    17161 ctgctatccg cccgcctttt caaaacaggt tccgacgatt ccgcacgcgc ctgccgcctt
    17221 tgccaagccg tacaggattt cctgcggcat atcgcggctc cataatcccg taatattcgc
    17281 aatcacgggc agatggctga tttggcggac tttcacgatg gattcgacat ccaaacggta
    17341 gggatggcct ttggtatggc ccaacacgcc cgactcgccc agaatcaggt ggcggttgcc
    17401 gcgcgagacg acatattctg cggcattcaa ccaatcttcg gcagaatggt gcttgtcttt
    17461 acacagaacc aagggaatgt tcaggcttcc ggcttcattc aatacggcaa gatcggacat
    17521 cagcccgccg cccaaataca ggatgtccgc tcccgcattc aaagccgctt cgacatggcg
    17581 gacgttgcga acgcgcacca atacgggttt ccctgcgcca tgcgccgatg cggcctgttc
    17641 cgccaaccgt ctgcaccgtc cccgcccttc atccgcactt aaagtgtcgt ataagtttgc
    17701 cgaagtgaaa aacggatcca gaaacactgc atccgcattg ctccatactg acggttctgc
    17761 agcgatacgg acggtttccc cgccgccgaa agccacgcct ttggcggcaa cgcggctgtc
    17821 ttccgcccga ttttcccgac tgacggtttt ccatgtgtcc aaaatgcgga cggctttctc
    17881 gacctccggc agcgtctgta cctccctgac gctcaaaacc ctatcgtcgc cgattgcgcc
    17941 gatgacggta cgctcgtcgc cgtgagaaat gtgttctcgc agacctctgc tgcggatgaa
    18001 ggcgacaacg ccggcaatgt ccgcttcggc ggcacgcctg ctcatgacaa taatcatatt
    18061 tcctcctgac acaagagacg gcccgcccaa aataggattt ttgcaagccg tgttatactg
    18121 tggcgtgttt tacagattgt tcgggctatg gatttattat cggttttcca caaataccgt
    18181 ctgaaatatg cggtggcggt gctgacgatg ctgcttttgg cggcaatcgg gctgcacgct
    18241 tccgtatatc gcaccttcac gcccgaaaac atccgcagcc gcctccaaca aagcattgcc
    18301 catacccacc ggaaaatctc gtttgatgcg gatatacggc gcaggcttct gccccgcccg
    18361 accgtcatcc tgaaaaacct gaccattacc gaacccgacg gcggccgggt cgccgtttcc
    18421 gtcaaagaaa ccaaaatcgg attgagctgg aaaaacctgt ggtcggatcg gatacaggtt
    18481 gaaaaatggg tggtttcggg tgcggatctt gccctgacgc gcgacagaaa cggcgcttgg
    18541 aacatccaag acctgttcga cggcgcgaaa cactccgcct cagtcaaccg cattatcgtc
    18601 gaaaacagca ccgtccgcct caatttcctg cagcaacagc ttatcctgaa ggaaatcagc
    18661 ctcaacctgc aatcccccga ttcgtcgggg cagcagtttg aaagttcggg catactggtt
    18721 tggagaaagc tgtccgtccc gtggaaaagc agggggctgt tcctttcaga cggcatcggc
    18781 acgcccgaaa tctcaccgtt ccattttgaa gcttccactt cgctggacgg acacggcatc
    18841 accatttcca ccaccggcag cccttctgtc cgcttcaacg ccggcggagc ggatgccgcc
    18901 ggcctcggcc tgcgtgcaga cacttccttc cgcaacctcc acctgaccgc gcaaatcccc
    18961 gcactggcac tcaaaaacaa cagcatcaaa accggcacgg tcaacggcac gtttaccgcc
    19021 ggcggcgaat atgcccgatg ggacggttcg ttcaaactcg acaaagccaa cctgcactcc
    19081 ggcatcgcca acatcggcaa cgccgaaatc tccggcagct tcaaaacacc gcgccttcaa
    19141 accaatttct ccctcggctc gccgttggtt tggagtcggg acaacgggct ggacgccccg
    19201 cgcctgcaca tatcgaccct tcaggatacc gtcgaccgcc tgccgcaacc ccgtttcatc
    19261 agccggctcg acggttcgct gtccataccg aatctgcaaa attggaatgc cgaattaaac
    19321 ggcacattcg accgccaacc cgttgccgca aaattcaaat atacgcggga aggcgcaccg
    19381 cgcctggaag ccgccgccgc gctgcaaaaa ttaaacctcg ccccctatct tgacgaattt
    19441 cggcaacaaa acggcaaaat attccccgac atcctcggca ggctgtccgg caacgtcgag
    19501 gcacacctca aaatcggcag catccaactc cccggcttgc aactggacga tatggaaacc
    19561 tacctccacg ccgacaaaga ccatatcgcg ctcagccgtt tcaagtcagg gctttacggc
    19621 ggccataccg aaggcggcat cagcatcgcc aacacccgtc ccgccactta ccgcctgcaa
    19681 cagaatgcaa gcaacatcca aatccaaccg ctgctgcaag acctgttcgg cttccacagc
    19741 ttcagcggca acggcgatgc ggtcatcgac ctgaccgcaa gcggcgaaaa ccgcaaacag
    19801 cttatccgct cgctgcaagg cagcctgtcg ctgaatattt ccaacggcgc gtggcacggc
    19861 atcgatatgg acagcatttt aaaaaacggc ctttccggaa aaatctcggg cagcacaccc
    19921 ttctaccgat tcacgctcaa cagcgaaatt tcagacggca tcagccgcca catcgatacc
    19981 gaactcttct ccgacagcct ctatgttacc agcaacggct ataccaatct ggatacgcag
    20041 gaattgtctg aagatgtcct tatccgcaac gccgtccatc cgaaaaacaa accgattccc
    20101 ctgaaaatca ccggtacggt ggacaagccg tccattaccg tcgattacgg caggctgacc
    20161 ggcggcatca attcgcgcaa agagaaacag aaaatcctcg aagacaccct gctggaacaa
    20221 tggcagtggc tcaaacctaa agaaccgtaa ataccctgcg tacaaaaatg ccgtctgaaa
    20281 cacattaatc ttcagacggc atgaccgtaa aacctacaac cccaatttcc cccaaatccc
    20341 atcaatttta accgtaaccg cagggtcttt tttgattacc cgtccccatt cgcggtcggt
    20401 ttctcccggc catttgctgg tcgcatccaa acccattttg ccgccgagtc cgctgacggg
    20461 gctggcgaag tcgaggtagt cgatgggcgt gttttccacc aaaacggtgt cgcggacggg
    20521 gtccatgcgc gtggttaccg cccagatgac ttctttccag tcgcgcacgt ttacatcgtc
    20581 atccaccacg atgatgaatt tggtgtacat aaactggcgc aggaacgacc agcagcccgt
    20641 catcacgcgc ttggcgtgtc cggcgtactg ttttttcatg ctgaccaccg ccatgcggta
    20701 ggaacagcct tcgggcggca ggtagaaatc ggtgatttcg gagaactgct tttgcaaaag
    20761 cggtacgaac acttcgttca acgccacgcc caaaacggca ggttcgtcgg gcggtttgcc
    20821 tgtgtaggta gagtggtaaa tcgggttttc gcgcatggtg atgcgttcga ccgtaaacac
    20881 agggaaatgg ccctgctcgt tgtaatagcc cgtgtggtcg ccgtatgggc cttccaacgc
    20941 ggtttcgttt ggatgaatca cgccttccaa tacaatttca gcacgggcag gcacttgcaa
    21001 atcgctgccg atacatttca ccagctccgt ccgcgaaccg cgcagcagtc cggcaaactg
    21061 gtattcgctc aaagtatcgg gaacgggcgt taccgcgccc aaaatggtgg aggggtcgca
    21121 accgagtacg acggcgacgg gatacggcgt atcgggattg agtttgcgga attcctgata
    21181 atccaacgcg ccgccgcgat gcgacagcca gcgcataacc agcttgtttt tgccgatgag
    21241 ttgttggcgg taaatgccga gattttggcg ttttttgtgc ggcccgcgcg tgacggtcaa
    21301 gccccacgtt accagcggcg caacgtcttc cggccagcaa tgttgaatcg gaagctgata
    21361 caaatcaacg tcttctcctt cccacacgat ttcctgacac ggcgcgtttt tcaccacgtt
    21421 cggcgccatg ctccaaatat ctttcaacag cggcagtttg gaaaacgcgt ctttgatgcc
    21481 tttgggcggt tcgggttctt tcaaatacgc cagcgtctgc ccgatttcgc gcagcttgga
    21541 cacgctgtcc gcgcccatgc ccatcgccac acgttcgggg gtgccgaaca ggttcgccaa
    21601 cacgggataa tcatagcgcg taccgtcggg cttaaccggg ttttcaaaca acaacgccgg
    21661 cccttcggcg cgcaacacgc ggtcggcaat ttcggtcatt tccaaatgcg gggaaacggg
    21721 gtgggcgacg cgcttgagct tgccctgctg ctcgagcata gcgatgaagt cgcgcaggtc
    21781 tttgtatttc atgtttatcc tttttgtcct tttatcctga acaatccgat tcggataccg
    21841 cccctatcct tgcctgcgct tcggcatatc ccatgccgtg ataaaagtcg cgtaccggcg
    21901 gatgtccgcc gctttgatga agttgcagca aagggcgttg gttgtcggac tgggtaacga
    21961 cattgcagtg cagaccgaag gtgtcggttt cataaggggg cagccgattg caaatcatac
    22021 cgaaataaac ggcgttttca gggttgtcgt aaaagcggct ttgatagtcg ttaaaactct
    22081 tttcgctgac ggacacccac acgccgtatt ccagcgtttc ctgatgcccg ataatcggaa
    22141 taggcagcgc cgcgcggata aagcggtcgg tttggccgga atagcggatg atgcagaaat
    22201 cagaatcgca ttccgcttga taagcaatgc gttcttcttc actgagttga ttatagggat
    22261 agggggcggt aaagccggtt gcgggcatat cttcatgttt ttcgccgcag gaagtgcaag
    22321 tgtgcataag gtttcagact ttcaacacaa gtttgcggta aagccattcg ccggcaaaca
    22381 gcatccccat caatacatag gcaatcacgc ctgtataaac cgcccaccaa tcatatcgcc
    22441 ccaaccgggc caacaaagcg gcaagcgtcc cgttggcaat aaagaatacg caccaaacct
    22501 gcgttacccg gcgggtatag cgcacggctt tttcaggcag gtcgggctgt tgcagccgcg
    22561 caagtttttc aatcaccgtc tgcccggcaa acaggctgct gccgaacacc gccaacatca
    22621 tcaaattaac gaggacagga taccaataca ttgaatcacg ccgcccgaac accaatactg
    22681 cggcaaaaaa cagtgtaata tagtggatta aatttaaatc aggacaaggc ggcgaagccg
    22741 cagacagtac aaatagtacg gcaaggcgag gcaacgccgt accggtttaa atttaatcca
    22801 ctataccaca aagcgggata ggcaatgctt aatacggtca aaaaaatatg tccgaaaaaa
    22861 ccgggtttca ttttgaatcc gcacaaacgt tttcagacgg catccgataa aaacatgccg
    22921 tctgaaaaat aattagaaat acccgattag ccggcctgaa tcttcaatac cgcctgtacc
    22981 acgtcgttga cggtgcggac attgcggaaa tcttcagcct gcagcttgcg ggctgccaca
    23041 accattcacg attgcgatat acccggcggc attcccgact gccgttcagc agaaaacgca
    23101 cccattccaa accgctccaa tatgcctcgg gcagcatacc ggcttcagac ggcatatcgt
    23161 ccgaagcaga caaagtcagg ctgtaacgcg tccctttggt cagaaccatc gccaaagcat
    23221 aagcaaacgg cgcgcgcgtt gccgatacgg catatccttc cggcagcgga tcgtccgccg
    23281 ccaaaaccaa aaccgacccg catccctctt ccaacaggga cgccgcttcc gccaatgccg
    23341 tttccacacc gtcggcacac acggccaatg ccgtctgctc gctcatatcc cgcctcaata
    23401 tcgaccattg ccccgccgtc gcattgtgca ccgacaaacc gaacgaagtc ggcgacacgg
    23461 tatgcgattt caacagttcc aaccacaaat cgaaactacg cgccatttcc ccgtcgtgcg
    23521 aggcataaac taccggactg ccgggatggg cggaggcaat gtcccaagcc gcgtcgcata
    23581 ccaaacgcgc cgccttactc aaacggcggc gccgcatagc gggcaggaac ggcaattccc
    23641 gcctgacatc gggcaaaccg tcggcaaaat ccggacattc cgcccactgc ctctactggg
    23701 ccatatcgcg cattttgccg cccgaaaccc gccagtcggc gatgtcgaag tggaaccggc
    23761 aaataatcgg cggcatagtt tctttcaaaa atttacactg tgccgcattc taaccaaagc
    23821 ccatccccct gacaatgccg aaattcaaac gcatccacgc cattttttcc gacaacgccg
    23881 cccctcggca aaccgcccga attagcctga atttacattt atcattataa tgcccgatta
    23941 ccgacaccgc ctccctgctg ccgcacagcg ggcgtatggt tctgatagac cgcattaccc
    24001 gatacggcga tgattttgtc gaagcagggg cgcaggtaaa acccgaccac atcctgctgg
    24061 ccggcggcat gctgccctat acggcattta tcgaactgat ggcgtaggct gtcggcgcat
    24121 atgccggcct ccaagcccga aaaaaggcgc aaccggtccg gctcggtttt cctgcccggc
    24181 acgcgcaaac ttgaaatttt tacccaatcc gtccctgtcg gaacacattt gccggcaacg
    24241 gcgcatatgt ctatccaaga tgccggggta tgggcgtgtc cgactgcgaa ctgcgttgga
    24301 cggacgcgcc ggaaaccttg tctgaaacac tcctttcaga cggcattttg gcgcgcgcct
    24361 cactcaacgt gtacagcccc gaacaccctg tcgaaacaac ttaatgccgt ctgaacaggc
    24421 acgcacatac ggagaacaac catgactgaa accgtcctga ttaccggctc aaacagaggc
    24481 ataggcaaag ccgccgcact cggttttggc ggacgacggc tttgatatcg ccgtccactg
    24541 ccgcagccgc cgcgacgaag ccgaagccgt ggcggaagaa atccgcgctt tgggcagaaa
    24601 tgcgcgcgtg ttgcagtttg acgtgtccga ccgcgaagcc tgccgcgaga ttctgaccgc
    24661 cgacatcgaa gcaaacggcg cgtattacgg cgtggtgttg aacgccggcc tgacgcgcga
    24721 caatgccttc cccgcgtttt cagacgacga ttgggatgtg gtgccgcgga ctaatttgga
    24781 cggtttttac aatgtactgc atccgctggt tatgccgatg atacgccgcc gcaaagccgg
    24841 acggattgtg tgtatggcat cggtgtccgg cctgacgggc aaccgggggc aggtcaatta
    24901 cagcgcgtca aaagcgggca ttatcggcgc ggcaaaagcc ttggcggtcg aaccggcgaa
    24961 acgcaaaatc accgtcaact gcgtggcgcc gggtttgatc gatacggaga ttgtcgatga
    25021 aaacgtaccc gtcggagaaa ttttgaaagc cgtccccgcc gcgcgtatgg gactgccgga
    25081 agaagtggcg cacgcagtgc acttcctgat ggatgaaaaa gcggcgtata tcacgcgcca
    25141 ggtgattgcg gtgaacggag gtttgtgttg aataccagaa gggtcgcagt aacgggcata
    25201 ggcggcatta ccgccttcgg ccgggattgg caaagcatac aggcagcatt caaagccgaa
    25261 aaaaacgccg tcaaatatat ggattggcgc gaacgtttcc ccgaattgga agcgcaactg
    25321 ggtgcgccga ttgaggatta cgcgccgccg aaacattgga cgcgcaagcg gctcagaagt
    25381 atggggcgcg tgtcgtacct gtgcgtcgat gcggcggagc aggcgctggc ggatgccggt
    25441 ttgctcggag acgaaagcat taccgacgga cggatgggcg ttgcctgcgg ctcttccggc
    25501 ggcggcacca aagacatcgg agatgtggga gaattgttgc tgaccggcac gtcgcgcaac
    25561 ttcggcgcca atacctatgt gcgtatgatg ccgcacaccg ccgacgccaa tatcggcatc
    25621 tttttcggat taacaaggac gcatcatccc gacatcgagc gcgtgttctt cgggcagcca
    25681 aggcataggt tatgcttacg aggccatcaa atacggtctg accgatatga tgctggcggg
    25741 cggcggcgaa gaattttgcc cgtccgaagt gtatgttttc gactcgctct atgccgccag
    25801 ccgccgcaac ggcgaaccgg aagagacccc gcgtccatac gacgcgaacc gcgacgggct
    25861 ggtcatcgga gaaaaggcgc ggggattttc gtattggaag aattggaaca cgccaaacgg
    25921 cgcggtgcga aaatttacgc cgaactcgtc ggctacggcg ccaacagcga cggcatcaat
    25981 atcggcgaag gcgcagccgt attcatcatg acgcgcgatg cggatttttc cggcggtatg
    26041 cagcttctgg gttacggcgc aagcagcgat gcctaccata tttccacgcc ccgccccgac
    26101 gcaaaggcgc aatcctcgcc tttcagacgg cattgcagca cgccggcctt gcgcccgaag
    26161 acatcggccg gattaatctg cacggcaccg ggacgcacca caacgacagt atggaaagcc
    26221 gcgccgttgc agcggttttc ggcaacaata cgccctgcac gtccgccaag ccgcaaaccg
    26281 gacacacgct gggcgcggcg ggcgcaatcg aagccgcgtt cgcgtggggc attgccgacc
    26341 ggcaaagcaa tcccgaaggg aaacttccgc cccggctttg ggacgggcag aacgaccccg
    26401 acctgcccgc cattaacctg accggcgacg gctgggaaac cgaaaaacgc attgccgcca
    26461 gctcgtcgtt tgccttcggg ggaagcaact gcgtcttaat catcggatga aataagtttg
    26521 tcaatcccac cgctatgcta tacaatacgc gcctactctt gacgggtctg tagctcaggg
    26581 gttagagcag gggactcata atcccttggt cgtgggttcg aaccccaccg gacccaccaa
    26641 ttcccaagcc cggacgtatg tttgggcttt tttgccgccc tgtgaaacca aaatgctttg
    26701 agaaaccttg ataatgaaaa aagtcagcgt attgattgtt gccaaaaacg aagcaaacca
    26761 cattcgggaa tgtattgaaa gttgccgttt cgataaagaa gttatcgtta tcgacgacca
    26821 tagcgccgac aacactgccg aaattgccga gggtttgggt gcaaaagtct tcagacggca
    26881 tttgaacggg gatttcggag cgcaaaaaac atttgccatc gaacaggcag gcggagaatg
    26941 ggttttcctg attgatgcag acgaacgctg cacgccggaa ctatctgatg aaatctcaaa
    27001 aattgtccga accggcgatt atgccgccta ttttgtcgaa cgccgcaacc ttttccccaa
    27061 ccatcccgcc acacacggcg cgatgcgtcc cgacagcgta tgccgtctga tgccgaaaaa
    27121 aggcggttcg gtgcaaggca aagtacacga aaccgtacag accccctacc ccgaacgccg
    27181 tctgaaacat tttatgtacc attacacgta cgacaactgg gaacaatatt tcaacaagtt
    27241 caacaaatat acttccatct cggccgagaa ataccgggag cagggaaagc ccgtgagttt
    27301 cgttagggac attatcctcc gtccgatttg gggttttttc aaaatttata tcctgaacaa
    27361 agggtttctt gatggaaaaa tgggttggat tatgtccgtc aaccacagct attacacgat
    27421 gattaaatat gtcaaactat attatctgta caaatccggc ggaaaatttt aaatggaaaa
    27481 agaattcagg atattaaata tcgtatcggc caagatttgg ggtgggggcg aacaatatgt
    27541 ctatgatgtt tcaaaagcat tgggacttcg gggctgcacc atgtttaccg ccgtcaataa
    27601 aaatgatgaa ttgatgcgca gacgcttttc cgaagtttct tccgttttca caacgcgcct
    27661 tcacacgttc aacgggctgt tttcgctcta cgcgcttacc cgctttatcc gggaaaacca
    27721 catttcccac ctgatgatac acaccggcaa aattgccgcc ttatccgtac ttttgaaaaa
    27781 actgaccggg gtgcgcctga tatttgtcaa acataatgtc gtcgccaaca aaaccgattt
    27841 ttatcaccgc ctgatacaga aaaacaccga ccgctttatt tgcgtttccc gtctggttta
    27901 cgatgtgcaa accgccgaca atccctttaa agaaaaatac cggattgttc ataacggtat
    27961 cgataccggc cggttccccc cgtctcaaga aaaacccgac agccgttttt ttaccgtcgc
    28021 ctacgccggc aggatcagtc cggaaaaagg attggaaaac ctgattgaag cctgtgtgat
    28081 actgcatcgg aaatatcctc aaatcaggct caaattggca gggcacggac atccggatta
    28141 tatgtgccgc ctgaagcggg gcgtatctgc ttcgggagca gaaccatttg tttcttttga
    28201 agggtttacc gaaaaacttg cttcgtttta ccgccaaagc gatgtcgtgg ttttgcccag
    28261 cctcgtcccg gaggcattcg gtttgtcatt atgcgaggcg atgtactgcc gaacggcggt
    28321 gatttccaat actttgggtg cgcaaaagga aattatcgaa catcatcaat cggggattct
    28381 gctggacagg cttacgcctg aatctttggc ggacgaaatc gaacgcctcg tcttaaaccc
    28441 cgaagcaaaa aacgcactgg caacggcagg acatcaatgc gtcgccgccc gtttcaccat
    28501 caaccatacc gccgacaaat tattggatgc aatataagcc gctttcagac ggcctatgcc
    28561 gtctgaaagc ctttgatgca acaaaccgcc aaattatatt cgttcattgg aaagaaacac
    28621 cccgaattca tccttcaaaa taagaaaatc ccaatatccc ccgatatcgc gcagcctatt
    28681 ggcaaagttt tgcagcgtct tccccggctt gtgccgccgc gtcaagtgct ttgttacaat
    28741 gcactacctt cacatttctt aataaatttt atgagtaacc atacttcttg gtcgtccaaa
    28801 atcggtttcg tacttgctgc ggcaggttcg gccatcggtt tgggcgcgat ttggaaattt
    28861 ccttatacgg cgggcaccaa cggcggcgcg gtgtttttcc tgctgttttt gatatttacc
    28921 gtcttggtcg ccctgcccgt ccagcttgcc gaattttata tcgggcgcac gggcggtaaa
    28981 aatgccgtcg attccttcag ggttctgcgt ccgggtacgc aatggctttg ggtcgggcgc
    29041 atgggcgttg ccgcctgctt tattttgctg tcgttttaca gcgtggtcgg cggatgggta
    29101 ttgaattatg tcgtccacag ttttacaggg gcaatccatg cgggagcgga ctttgaagcc
    29161 ctgttcggca caacgatttc caatccggca ggttcgctgt cctatcaggc actgtttatg
    29221 ctgattacgg tttgggtggt caaaggcggc atttcagacg gcattgaaaa ggcaaaccgt
    29281 tatctgatgc cggggctgtt tatcctcttt attgcgctgg ctgtccgttc gctgacgctg
    29341 ccggatgcaa tggagggcgt gtctttcctg ctcaaaccca attggtcgta ctttaaagcc
    29401 gatacgatga ttacggcttt gggccaggcg ttttttgccc tgagcatcgg cgtttccgcc
    29461 atgattacct atgcttcgta tttgggaaaa gatcaggata tgttccgttc cggccatacg
    29521 attatgtgga tgaacctctt ggtttccctg cttgccggcc tggtgatttt cccggcggta
    29581 ttcgccttcg gttttgaacc gaaccagggg ccgggattga tttttatcgt attgcccgca
    29641 gtgttcatga aaatgccgtt cggtacggtt ttatttgcag tattcatgct tctggtcgtt
    29701 ttcgccacgc tgacttcggc attttcgatg ttggaaacgg tcattgcctc aaccatccgc
    29761 caagacgagc gcaaacgcaa aaaacatact tggcttatcg gcacggctat tttcattgtc
    29821 ggcatcccgt ccgcgctgtc tttcggcgca tggggtgagt ttaaggtttt cggcaaaacc
    29881 atttttgatt tgtgggacta tgttatttcc gccgtcatta tgccgattgg tgctttgagt
    29941 gtttccatct ttaccgcctg gattcaggac aagcagtctg tgttaaaaga tgccggcgcg
    30001 ggcagcaccg taccacgggc agtgctgctg ctgtggctga ataccttgcg ctaccttgcc
    30061 ccgattgcca ttattattgt tttcgtcaat tctttaggca tcctttaaac ataccgcccg
    30121 acggcaaaat gccgtctgaa agcctttcag acggcatttt gcttcagatt caacctattt
    30181 cgttcaaagt atagtggatt aaatttaaat caggacaagg cggcgaagtc gcagacagta
    30241 cagatagtac ggcaaggcga ggcaacgccg taccggtttt tgttaatcca ctatattgtc
    30301 cggcatggcc agcctaactt taagccgcct gctttcggca accgaccgta tcagcggcaa
    30361 atccatcacg gaaaacgccg tcgacaaggc atcggcaacc gccgcatcat ccgccattac
    30421 gctcatgctt ttataacgcg gcgtactcac gccggttcgc ggatcgaaca aatgggtaag
    30481 cctgcccgct ttgtccatca ccgtcccata gccgcccgaa gtggcaaacg ccttgtcttt
    30541 catcgtgata tttgccaaca cgccttcttc gtcgtccgga ttgcggatgc cgacattcca
    30601 catccgcctg ccgtttgtat caaaccccct gatttcgccc atatcgacca gcgcggcagg
    30661 cactccgttt gctttaagca gcgcaacaac cttgtccgtg atatagcctt gcgcgatgcc
    30721 gttcaaggac aaacccatac ccttttcggc aaaacggatt tcacggtcgt caaacgagac
    30781 tttgccgaag ccgacccgtt tcagggtttc cttcacgcta ttctccggcg gcgtttccgc
    30841 atcgggacgg ccggcaaaat aatcggcata cagtttccac aacgcctgaa ccgtcggatc
    30901 gaacgcgcct tccgtcagcg cgtgaatatc gcggccaggc tcaacagttc caaaaaatcc
    30961 gccggaggcg aagtcagata accgtcgcgg ttcagacggc tgatcaggct gtcttcacgg
    31021 taaaggctga acattttttc caaacgcgcc acttccgcca aaaccttatt gaccaaatcc
    31081 gccgcctgtc tgtcgtccac gccgaacagg cgcagctccg cgccggaacc cagcgcgaca
    31141 cctttccaga aaaacacatt ttcatcgcgt tttttatcca tgttgcgttt ttcgccgccg
    31201 gcggcaaaag gattcggcaa aaaagaaacc gccgcaccgg ccacagcaac ggcggcagcc
    31261 gtcagaaaac gtctgcgccc gaaatgcctg cccataccgc ctcctaaacc gacactaccg
    31321 ccttgatgtg cggatgcgga tcgtagcctt ccaactcgaa atcttcaaac ttgaaggcaa
    31381 acaaatcctt gacttcagga ttgattttca tcacaggcaa ggcgcgcggt tcgcgttcca
    31441 actgcaatgc ggcctgttcg aaatggttac ggtacaaatg cgcgtcgcca aacgtatgga
    31501 caaactcgcc cgcctccagc ccgcacactt gcgccatcat catcgtcaac agcgcgtagc
    31561 tggcaatatt aaacggcaca ccaaggaaaa tatctgcact acgctggtaa agctggcagg
    31621 acagtttgcc gtcggcaacg taaaactgaa acagcgcgtg gcagggcggc aaggccatct
    31681 catcgaccaa agccggattc cacgccgaca caatcaggcg gcgcgagtcg gggtttttct
    31741 taatttgttc caccacattg gcgatttggt cgatatgcct gccgtcgggc gcgggccagc
    31801 tgcgccattg gtagccgtaa accggaccca agtcgccgtt ttcgtccgcc cactcgtccc
    31861 aaatggaaac attgttatcc ttcaggtatt tgatattggt atcgcctttg aggaaccaaa
    31921 gcagctcgtg gattatcgag cgcaaatgca gctttttggt cgtcagcagc ggaaaacctt
    31981 tgcccaagtc aaaacgcatc tgataaccga atacggagcg cgtacccgta ccggtgcggt
    32041 ctgatttgtc cgtaccgttg tcgaggacgt ggcgcatcaa gtccaaatag gccttcatca
    32101 tcatctttca tcaaattaaa cggcacatat tgtaacattt ccggccaaca cccgagtccg
    32161 gacaaacgcg caggccgccc cgccggacgc tcaaaacgcg gatgaaggca ggcaggattg
    32221 ttggcaattt cagccctttt tcacagcaaa agcaggcgtg aaaacaaaat tatcttgatt
    32281 ggaatcaaaa aa
//
LOCUS       DAWTHD010000011        29745 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWTHD010000011 DAWTHD010000000
VERSION     DAWTHD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29745
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.011"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            58..339
                     /locus_tag="WM704_000400"
     CDS             58..339
                     /locus_tag="WM704_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339900.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            336..1379
                     /locus_tag="WM704_000401"
     CDS             336..1379
                     /locus_tag="WM704_000401"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFA9339901.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGMSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
     gene            complement(1973..4015)
                     /gene="recG"
                     /locus_tag="WM704_000402"
     CDS             complement(1973..4015)
                     /gene="recG"
                     /locus_tag="WM704_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFA9339902.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(4101..4544)
                     /gene="secB"
                     /locus_tag="WM704_000403"
     CDS             complement(4101..4544)
                     /gene="secB"
                     /locus_tag="WM704_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFA9339903.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(4565..4822)
                     /gene="grxC"
                     /locus_tag="WM704_000404"
     CDS             complement(4565..4822)
                     /gene="grxC"
                     /locus_tag="WM704_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFA9339904.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5041..6534
                     /gene="rng"
                     /locus_tag="WM704_000405"
     CDS             5041..6534
                     /gene="rng"
                     /locus_tag="WM704_000405"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFA9339905.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(6569..7820)
                     /locus_tag="WM704_000406"
                     /pseudo
     CDS             complement(6569..7820)
                     /locus_tag="WM704_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
     gene            complement(7902..8531)
                     /locus_tag="WM704_000407"
                     /pseudo
     CDS             complement(7902..8531)
                     /locus_tag="WM704_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(8799..10112)
                     /locus_tag="WM704_000408"
     CDS             complement(8799..10112)
                     /locus_tag="WM704_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFA9339906.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(10490..11062)
                     /locus_tag="WM704_000409"
     CDS             complement(10490..11062)
                     /locus_tag="WM704_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFA9339907.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            11221..12630
                     /gene="dacB"
                     /locus_tag="WM704_000410"
     CDS             11221..12630
                     /gene="dacB"
                     /locus_tag="WM704_000410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFA9339908.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(12695..13864)
                     /locus_tag="WM704_000411"
     CDS             complement(12695..13864)
                     /locus_tag="WM704_000411"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFA9339909.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(14047..14157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            14210..15103
                     /locus_tag="WM704_000412"
     CDS             14210..15103
                     /locus_tag="WM704_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFA9339910.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(15148..16212)
                     /gene="tsaD"
                     /locus_tag="WM704_000413"
     CDS             complement(15148..16212)
                     /gene="tsaD"
                     /locus_tag="WM704_000413"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFA9339911.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(16328..17515)
                     /gene="ccsB"
                     /locus_tag="WM704_000414"
     CDS             complement(16328..17515)
                     /gene="ccsB"
                     /locus_tag="WM704_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFA9339912.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATVLGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(17508..19460)
                     /locus_tag="WM704_000415"
     CDS             complement(17508..19460)
                     /locus_tag="WM704_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HFA9339913.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKGTGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(19738..20361)
                     /locus_tag="WM704_000416"
     CDS             complement(19738..20361)
                     /locus_tag="WM704_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HFA9339914.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            20568..21197
                     /locus_tag="WM704_000417"
     CDS             20568..21197
                     /locus_tag="WM704_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HFA9339915.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(21318..23714)
                     /locus_tag="WM704_000418"
     CDS             complement(21318..23714)
                     /locus_tag="WM704_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFA9339916.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLPQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            23866..24981
                     /locus_tag="WM704_000419"
     CDS             23866..24981
                     /locus_tag="WM704_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFA9339917.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSAEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            24984..25583
                     /locus_tag="WM704_000420"
     CDS             24984..25583
                     /locus_tag="WM704_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFA9339918.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            25584..26231
                     /gene="pilO"
                     /locus_tag="WM704_000421"
     CDS             25584..26231
                     /gene="pilO"
                     /locus_tag="WM704_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFA9339919.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            26249..26794
                     /locus_tag="WM704_000422"
     CDS             26249..26794
                     /locus_tag="WM704_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFA9339920.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPVAEQN"
     gene            26813..29008
                     /locus_tag="WM704_000423"
     CDS             26813..29008
                     /locus_tag="WM704_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFA9339921.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGNKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGSNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
BASE COUNT         7464 a         8646 c         7180 g         6455 t
ORIGIN      
        1 tttcagacga ccttttgcta taatcgggct tcatcgcccc gttcggtttg gaaccttatg
       61 aaaaccctcg tcctcctcct gctttccttc tccacgacca ccgctttcgc cgcatacggt
      121 ttgggtttgg ggcaggcacc gaaatatcct gccggctttc gcgcctacgg ttatgtttat
      181 tccggacggc agggctgggt tttaaaaaca gaggcggatg ccattaaatt agacacgctt
      241 ttcaaacgct ttgtgtaccg tccttccgcc gccaatcaaa accccgtcgg acagcgttcg
      301 gacggcatac ccgccaacca cacaaaggaa aaaccatgag caaaaaaatc aaagccggca
      361 ttgtcggcgc gacgggctac acgggcgtgg aactgctgcg cctgcttgcc gcccatccag
      421 atgtcgaagt cgccgccgta accagccgca gcgaagcggg aaccgcagtt gccgattact
      481 ttccaagtct gcgcggcgtg tacggcctcg ccttccaaac gcccgacgag gcaggtttgg
      541 aacaatgcga catcgtcttc ttcgccacgc ccaacggcat cgccatgaaa gacgcgccgc
      601 gcctgattga acagggcgtg cgcgtcatcg acctttccgc cgacttccgc atacgggaca
      661 ttccgacctg ggaacactgg tacggcatga cccacgccgc ccccggcctc gtttcccaag
      721 ccgtgtacgg attgagcgaa ctcaaccgcg aagccgtcgc acaggcgcgc ctcgtcgcca
      781 accccggctg ctacccgacc tgcgtatccc tgccgctcgt gccgctgttg cggcaatgcc
      841 gtctgaagcc cggtatgccg ctgattgccg actgcaaatc cggcatgtcc ggcgcgggca
      901 ggaaaggcaa tgtcggttcg ctgttgtgcg aagccggcga caacttcaaa gcctacggca
      961 cagccggaca ccgccacctg cccgaaatca ggcagaccat cgccgggctt caggacggca
     1021 tcgccgaagg attcgtgttc acgccgcacc tcgcgccgat gatacgcggt atgcacgcca
     1081 ccgtttacct ccacctttca gacggcagcg accccgaaac cgtcctgcgc gactactacc
     1141 gcgacagccc gttcatggac atcctgcccg ccggttcgac acccgaaacc cgcagcgtgc
     1201 gcggcgcaaa cctctgccgc atcagcatcc gtcaggcggc gcaatccgat gtgtgggtcg
     1261 tcctttccgt catcgacaac ctcgtcaaag gcgcggcggg tcaggcagtc caaaatatga
     1321 acattatgtt cggactggag gaaacacacg gcttgggcgg catccccctg ctcccctgaa
     1381 gcgcaaacgg caaaccgcag gcatcgtgcc tgcggttttt tgatgccgtc tgaaagcggc
     1441 ggtttttttg ggttcggacg gcttttggct catcattcag cgaaaacaaa aatctaaaat
     1501 accgtcattc ccgcaaaagc gggaatctag tttatccggc ttcagcaatt tccgacacat
     1561 ttccacacgc ttcgattccg tcatttctcc ggtttcaatc gtttccgata acaccgtggt
     1621 ttttcatttc tagattcccg cctgcgcggg aatgacggcg gggggcttgc cgttttcccg
     1681 ataagcctct gcggattctc attgccggat ttccgcccgc ttgggaataa cgggatttaa
     1741 aatcatgaaa atgtgtcaaa aataatatag tggattaaca aaaaccggta cggcgttgcc
     1801 ccgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg aatcggttcc
     1861 gtactatttg tactgcctgc ggcttcgccg ccttgtcctg atttttgtta atccactata
     1921 aaaaatcaga tttccgttac acttttttct aatatttcag acggcatttt gctcacacac
     1981 ccaaataacc ttccctgccg gaaagccacc ttgccaaatg cgcttcgacg atttcagggt
     2041 tttgttcaat cagcatcggg gcggtttcgc gcgcttgttc caaaaggtgt aagtcttctt
     2101 cgagcttggc gaagcgcagc ataggcacgc cgctttggcg cgcgccgaga aattcgccgg
     2161 ggccgcggat attgaggtct tggcgggcga tttcgaagcc gtcggtgtgt tcgtagatga
     2221 ctttcagccg cgctttggcg agttcgccca agggttcggc aaacaggagg acgcacacgc
     2281 tttcccccgc gccgcgccct acccgtccgc gtaattggtg aagctgcgcc aagcccatgc
     2341 gctcggcgtg ttcgatcacc atcagggcgg cgttgggcac atctacgccg acttcgatga
     2401 cggtggtggc aaccaagacg ttcagacggc ctgcggcaaa ttccgccatg acttcggcct
     2461 tttcggcggc cttcatgcgc ccgtgtacca gtccgatatt gggttcgggc aatgccgtct
     2521 gaagccgggc gagggtttcg gcggcggttt gcagttgcag ggtttcgctt tcttcaatca
     2581 atgggcagac ccaatatgcc tgccgccctt ttcggcaagt gccgaggacg aagccttcga
     2641 cttcggcgcg gcggacgttg ttgacgaggc gcgttttaat cggagtgcgc ccgggcggca
     2701 attcgtcgat gacggatacg tccaagtcag cgaagaaact catggcgagc gtgcgcggga
     2761 tgggtgtggc ggacatcatc agctgatgga cttcgcgccc tttgtttttg agtgcgaggc
     2821 gttgtgcaac gccgaaacgg tgctgttcgt ccacaatgct caagcccaaa ttgtgaaacg
     2881 ccacgccgtc tgaaaacagg gcgtgcgtgc cgacggcgat tttgacgctg ccgtcggcga
     2941 gtttggcttt ggcttcgtct ttggcttttt tgcgcaaact gccaaaaagg cagacaactt
     3001 caatgcccaa aggttcgagc cattgtttga acttgatgaa gtgctgttcg gcaaggattt
     3061 cagtgggcgc cattacagcc acttgcgcgc ctgattcgat ggcggtcagc gcggataagg
     3121 cggcgacgat ggttttgccg ctgccgacat cgccttgcag caggcggtgc atggggtggg
     3181 tttgcgccat atcgcggcag atttcggaaa caactttttc ttgtgcatcg gtcagggcaa
     3241 acggcagggc ttggcgcagg gcttgggtca atgtgccgtc gccgcgcaat gccgccgccg
     3301 tgccgctgac gcgcttctgc cgcgccaaac gcatggacaa ctgttgcgcc aaaagttcgt
     3361 cgaatttgag ccgctgccac gccggcagcg tgccgtctga aagttgatga atagtgaaac
     3421 tcggcggcgg cgaatgcaaa aggcgcaggc tttcggcaag gcgcggcagc ttcagacggc
     3481 ctaataaagc atcaggcaag gtgtcgtgca gcggcgtaac gtccaacgcc gtctgaatga
     3541 tgcggcgcaa agtgggctgg ttcaaaccgt ttacggtcgg gtaaaccggc gtgaggcttt
     3601 ccgccaaacc gccgccctcg gcatcgcgga ttttgggatg aatcatctcg tcgccgtaaa
     3661 atccgtgttt gatttcgccc acggcgcgga tgcgtttgcc gaccgccgtc tgcttctgat
     3721 ggctggcgta aaagtggatg aagcgcagaa aaaggacgct gccggaaccg tcggcaattt
     3781 gaacaatcag ctgcttgcgc ggtttgaacg ttacttcctg atggataacc tcgccctcga
     3841 cctgacacgg cacgccgatc ggcgcgtcct taatcggcat aatgtgcgtc tcgtcctcgt
     3901 aacgcagcgg caggtgcaac accaaatccc acgcggtatg gaggttgagt ttgtcgagct
     3961 tcttggcgga aacatcggtg attttgagct gtttctgggt ttcgggcgac atcataggca
     4021 gattcctttg gacgcgccta ttttatccga aaacaaaaat gccgtctgaa acggattcag
     4081 acggcatcgg caggcaggaa tcaagccccg gcggcttcgg cttcctgctg ttgttggtaa
     4141 atcgcttcaa aattaatcgg cgcaagcagg acgggcggga agccggcgcg cgttaccgta
     4201 ccggaaaccg cttcgcgcgc gtaagggaag aggatgttcg gacacgccac gcccaacagc
     4261 agctgcacat cttcttcggg gatgttttcc agacggaaaa taccgctttg ggttacttcg
     4321 ttcaaaaaca tcgtgcgctc gttatccaat ttggcggtaa cggttacggt aacgtccacg
     4381 tcgtagtagc cgtcttccag cttttggctg ccggtggaaa cgcgcatatc cacttcggga
     4441 tcgccctgtt ccaaaaagat ttgcggcgcg tgcggcactt ccaaagacaa gtctttgaca
     4501 tacagtcgct cgatgctgaa tacgggttgc agttcttcgc tcattttgtt ttcctagttg
     4561 tgggttaagg gttcagcagt ccgtccagcc cgccttcctg ctgaaggcgg tagaggtcgg
     4621 taaatcctcc gacgtgcgtt tcgccgatga aaatctgcgg cacgctgcgc tgtcccgaaa
     4681 gccgctgcat ttcggcaaag gcttcggggc ttgcatcgac acggatttcg tcgatatgtc
     4741 cgacacctgc cgcgtgcagc agccttttcg ccatcgcgca gtaggggcaa aacggacctg
     4801 tgtacatggt aacggtctgc atattgggtt tccgaaagtt ttgcaatgat aatcaatata
     4861 ggggcaatcg ctccgtttgg caagtgcgga acagatgcac gttcagacgg catgtgcgga
     4921 gtgtgtcaaa gtttcttttt taaagtatga taggcattgt gaaaaatatt tttgcacccg
     4981 ctctgcgcgg cggggcggaa cggatgcaaa aatattttta ttacattttc aggaaaaacc
     5041 atgttgtcag gactccccat ccccaaagac atcgcgcgcc cgcccgaaac gatattggtc
     5101 aacatcacgc cgcaggaaac gcgcgtggcg gtgttggagg aaaacaatat ctgcgagctg
     5161 cacatcgagc gcaacagcgg gcacagcctg gtcggcaata tctatctggg cgtggtgcgc
     5221 cgcgtgctgc ccgggatgca gagcgcgttt atcgacatcg gcttggaacg cgcggcgttt
     5281 ttacacatcg tcgatgtcct cgaacaacgc cgcaaccccg aagaaaccca gcgcatcgaa
     5341 catatgctgt ttgaagggca gtcggttttg gtgcaggtca tcaaagaccc gatcaacacc
     5401 aaaggcgcgc ggctttccac ccaaatctcg ctggcggggc gtttcctcgt ccaccttccg
     5461 caagaagacc acatcggcgt gtcccaacgc atcgaagacg atgccgaacg cagcagcctg
     5521 cgcgaacgcc tcgacaagct cctgccggaa aatgcctgcc ggggctacat catccgcacc
     5581 aacgccgaaa acgccaccga cgaacagctc cagtccgaca tcgactacct gaccaaagtg
     5641 tgggaacaca tccaagaaca ggcgaaaatc cggccgccgg aaaccctgct ttatcaggat
     5701 ttgcctttaa gcctgcgcgt gttgcgcgat atggtcggct gcgacacgca gaaaatcctc
     5761 gtcgattcca ccgtaaacca cgggcgcatg acgcgttttg ccgaacaata cgtccacggc
     5821 gcattgggca ggatagaatt gttcaaaggc gaacgcccgc tgtttgaaac ccacaacatc
     5881 gaacaggaaa tcagccgcgc cctgcaaccg cgcgtcaacc tcaacttcgg cagctacctg
     5941 attatcgagt ccaccgaagc catgaccacc atcgatgtca acaccggcgg cttcgtcggc
     6001 gcgcgcaatt tcgacgaaac catcttccgc accaacctcg aagcctgcca caccatcgcc
     6061 cgcgaattaa ggctgcgcaa ccttggcggc atcatcatca tcgacttcat cgatatggcg
     6121 caggaaagcc accgcgaagc cgtgttgcag gagcttgcca aagccctcgc cttcgaccgt
     6181 acccgcgtta ccctgcacgg ttttaccagc ctggggctgg tcgagctgac gcgcaaacgc
     6241 tcgcgcgaaa acttaaacca agtcctctgc gaaccctgcc cttcctgcca aggcaggggc
     6301 cgcctgaaaa cgccgcaaac cgtatgctac gaaatccagc gcgaaatcgt ccgcgaagcg
     6361 cgccgttacg atgccgaaag tttccgcatc ctcgccgccc ccaacgtcat cgatttgttt
     6421 ttggacgaag aatcgcaatc cttggcaatg ctgatagatt tcatcggcaa accgatttct
     6481 ttggcggtcg aaaccgctta cacgcaggaa caatacgata ttgtgctgat gtaaaaaatg
     6541 ccgtctgaag ccttcagacg gcatctgtct atttcagggt ttccttgtcc aacagcgcgc
     6601 gtatcaacag cccgtgtccg aaacgtcggc tgtcggtcag ttccaaatat ccgccgtatt
     6661 ttttggccag cgtgtcggca atcgacagcc ccagccccgc cccctgctgc tccgttccca
     6721 aaatacggta aaacggatcg aggacgcggg cgcattcgga ttcgggaatg ccgttcccgt
     6781 tatcctccac ccacacggcg agatatttcc cttcgtccgt gaaacccaaa tctatcctgc
     6841 ccccgttcgg cgtgtaacgt accgcgttgt cggcaaaggt tttaaccagc gtatagattt
     6901 ccgtttcgtc ggcagacact tcgacatcgc ctccgaccgc cacaccgatg tcctgacgtt
     6961 tttccaaaac cagcggcatc agttcctgca acacttggcg gaaacggctt tgcagaccga
     7021 atgtcgtttt cgccaagggg ggtttcgtcc gactgcgaac gcgccagtgc caaaagctgt
     7081 tcgagcaggt gtttgttgcg cctgatgctc tgctgcaaaa cggcggactg ccgccccgca
     7141 tcgggtggga gcggcatatt gttgagccgt tccgcctgaa gggaaagggc agtcatcggc
     7201 gtacgcaatt cgtgtgccgc gtcggcgaca aaccgctgac ggcggcgtat atcttcatca
     7261 acacgtttca aaagcagatt gatggcggtt acgaaccctc tgatttcact gggaatattg
     7321 tacacactca aagcaggcag gccgttgatt cggcgttgtt cgagactttg cgacaatttg
     7381 cggacggggc gcatggcttt gtgcgtaatc cacacagtca gcagtatcat cagcggcagt
     7441 gccgccaaca ggggcaacac gctttgccgt gccgcatcct ccgccaaatc ttcacggtat
     7501 tcgttttcct gcatgacggc aatccgtccc tgttcggtcg tgcggatata ggcgcggtaa
     7561 taatcgtcgt ccccgtccgc ccgaagcgtg tgcagaccgt ccgccagatg cgcgggcagg
     7621 ctgacaacag ggtcttcctg ctgcggcatc tgtaccaaaa tacgcgtatc gccgtcgcct
     7681 tcgggcaaag tttcggattt ggaatcaggg ccgacataca atgccgcctg acggagcagg
     7741 tcgtcctgca acgcttccgt ttcgtggaag gtttcgtagt aggaaaacat acctgcaagc
     7801 attgccagcg gaacaaacat ccaaatcagg gcaaggctga ttctgacctg aagcgaatgt
     7861 tttaaaattt taaaaaaacg gtccggcatc ttcgttcctg ttcagacggc atcttgacgc
     7921 ggcatcagcc agccgacacc gcgcacattt tgtatgcttt ccttacccaa tttcttgcac
     7981 agcccgtgaa tcagaaaatc caccgcattg ctttcgactt ccccgcccca accgtaaacc
     8041 ttgtcctccg aatccgagcg cgacaaaatc acacccggcc tcgccagcaa agcctgcaat
     8101 accgaaaact cctggttgct caatgccacc tgcctcccct cggcaataat ttctacctga
     8161 tacgttgcag gattgaggga taacgcacca tttgacagac acgcctgcgc ctgtccgctc
     8221 ccgcgccgca acaccgcccg catccgcgcc ttaaactccg ccatatcgaa cggcttgacg
     8281 atatagtcat ccgcaccgcc gtccaaaccg ttcagccggc tgtacaaatc atcccgcgcc
     8341 gtaacgatca gcacggggac ggtacagcct gccgcgcgta tttccgacaa aacatccaaa
     8401 ccgtcccgcc caggcaaacc caaatccagc agcatcaagt cataaggctg ggcagcggca
     8461 gccgccgcaa cctacgcgcc gtttttgacc caatccaccg catagccgcc gtctttcaaa
     8521 cttgccgaca ccgcttacgc aatcatcgca ttgtcttcca ccagcaaaac acgcatcaac
     8581 tttcccttca aaataaaccg tgcctattct aacaccccaa aattagccgc aatttagcgg
     8641 cctttacgct tgccggtatt tttcaaaact gcggcacaaa aaaaccgcgc cggcaactgc
     8701 cttcagacgg cattggggcg cgattgcaac acacggacag gcaggcaaag cctgcgacag
     8761 atcacaggaa cgattcgggc ttcaggcggc ttcgccgttt acggcagagg cacgattcct
     8821 gccgctatcg aactgatcaa tatcgccagc gacaaacccc acgcccagaa aaacgaataa
     8881 cggatgtgtt tgcccatcga caatttcgcc aaacccaaac ccatccacaa agccggcgaa
     8941 agcggcgtaa caaaagtacc gacgatactg ccgatcaaca tcgcatagcc tgccgcttcg
     9001 ggtgcaacgc ccgcctgcga ggtaatctgt tccacaatcg gaaacagtcc gaaataataa
     9061 gcgtccgtac tcaaaaccaa ctcaagcgga atacccaaca caccgatggc aatatgcaga
     9121 taaggcagca acgcgtccgg caggatatgc acaaggtctt tggaaatcgc gtccaacatc
     9181 cccgcgccct tcaaaatccc caaaaacgta cctgccgcca aaataatgga cgccatcatc
     9241 accgcgccgc cggcgtgggc ataaatccgc tccatctgtt cctgcgggct gcggtaattc
     9301 aaaagcaacg ccgccgttgc agccagcata aatacataac ccggcgggaa gatgcccgaa
     9361 aaaagcaggc tcatcgccgc caaaaacagc aggacattcc accaaaacag tttcggacgc
     9421 gccaattttt gttcttcttc cgacaaaggc gccggcttta tcaaatccgc cacggcgggc
     9481 aacgcgccca actcccggac aatccgcctt ttttcacgca cacccaaaaa cagggacagc
     9541 acaaggataa acaccacacc gataatttgc accgtcaaca aaggtttata caattcgccc
     9601 acatctgcgc ccaacacgct tgcaacccgc ccgatcggcc cgccccgcgg caaaaggttg
     9661 attagccccg cgctggaagt cagcagcaaa aacagcaggt aaggattcat atgcagacgc
     9721 ttgtaaagcg gcaaaagggc ggggacgacc gataaaaacg tcgtcgcgcc cgccccgtcc
     9781 aactgtgcca ccaccgacac caagaccgtc cccacactca ctgccacgat attaccccga
     9841 gtcagcttaa tcaaaccgcc tatcatcgga cggaacagcc ccacatcgtt catgattcca
     9901 aaaaacaaaa tggaaaacat aaacataatc acaatctgcg tcaccgattt cgtgccgccc
     9961 gaataaaatt cttttaattg ggatacatca aaccccgcca gcaacgcccc aatcagcggc
    10021 accaagatta atgcgatgat gggcgacact ttttccgtca gcaacagcca tacgatgacc
    10081 ccgataatca gcaatccgat aaacgtcagc attatttctc ctttatttta ttttgaacat
    10141 aaaaccgccc gtgcaggcaa aaccgccaac atatgcggga taagccttgc attccattca
    10201 cgcctgccga catttctgca tccgtccgtc atttcacggc ggcaaccgat acggaacaac
    10261 cggcaaaccg gtatcgggac ggcgcggggg catccgtccc ggtgcaccga ttcaaacgaa
    10321 actgccccct atcattgcgg agcgcggggc gtgccgtaca cgcgggattt taaacacgcc
    10381 gatcatttgc cgcgcattat ccggcaaacg gcaaaccttg acgctgcccg gcccatataa
    10441 aaaagccgca aaacccgaac cggttttgcg gcttttcgat ttggtttgtt tatttggcat
    10501 ttctttcaat caaaccgaca aactgattga tatatgactg aacaaaatcc cttgccgagt
    10561 caatcaactt accgttttca tcaaacagcg tcggcgaatt gcccaaaaat acttccggct
    10621 gtccggttac gggcatatca aaatacgaca gggcaaggcg caggtttttt tgggaactgt
    10681 aaccgcccat cttgccgacg gaatggctga tgatgccggc cggtttgttt ttccaagcca
    10741 cgtcggcatt cggtttcgag ccgatgtcca ccgcattttt caaacaggcg ggaatggtgc
    10801 ggttgttttc ggacgtaacg aacaaaatgc cgtccgaagc cttaatcgtt tcgcggaaag
    10861 ccgtgtagct ttcgggcagc ggcacatctt ccaccgcagg gtcgtcataa tcgaaattgt
    10921 aaagcggcag atgtccgatt tcgacgattt ccgcctgcca gccttcgggg aacatctccg
    10981 ccgcattcaa tgccactttg cgcgcaaacg aagcacggcg caggctgccc accaaaatac
    11041 tgattttctt agccacaatc attcctcctg aatattaagt ttgtgcgttt caatcatttc
    11101 ataatggtaa cgattattat atatgtgatt tcccctgcaa acaagccggt ccgccgcccc
    11161 ggcgttccca cttatccggc tttgccttat aattgctttt tttatgtaac agattcacct
    11221 atgaatttcc ccaaaacagc ggcctccctg ctgctgcttc tcgcctccct cgccgcacac
    11281 gcgctcgata ccggccgcat tccgcaaaac gaaatcgctg tatatgtcca agagcttgac
    11341 agcggaaaag tcatcattga ccaccgtgcc ggcatacccg tcaatcccgc gtccacgatg
    11401 aagctcgtta ccgcgtttgc cgccttcaaa accttcggca gcaattaccg ctgggcgacc
    11461 gagtttaaaa gcaacggtac ggtaaacgac ggcacgcttg acggaaacct gtattgggcg
    11521 ggcagcggcg accccgtttt caatcaggaa aacctgcttg ccgtccaacg ccagttgcgc
    11581 gacaaaggca tccgcaatat cacggggcgc ctgatgctcg accacagcct gtggggcgaa
    11641 gtcggcagtc ccgaccattt tgaagccgac agcggttcgc cgtttatgac gcccccaaat
    11701 ccgactatgc tgtctgccgg tatggttatg gtgcgcgccg aacgcaatgc cgccggcagt
    11761 accgacatcc tcaccgatcc gcctttgccg catatttttg cccaaaacaa cttgaaaatt
    11821 accgcctccc aagctgcctg cccttcggtc aaaaaactga tgcgcgcatc tttttcgggc
    11881 aatacgctga aattgcgcgg caatattccc gaaagctgtt tgggcaagcc tgtcggtgtc
    11941 cggatgttcg cgcttgacga actgatccgc caaagtttta ccaaccgctg gctgctcggc
    12001 ggcggacgga tttcagacgg catcggcata gccgacacac cggaaggcgc gcagacgctt
    12061 gccgttgcac actccaaacc gatgaaggaa attttgacgg acatgaacaa gcgttcggac
    12121 aatctgattg cgcgttccgt cttcctcaaa ctcggcggcg acggcaaact gcccgccgtt
    12181 tccgaacagg cggcgtctgc cgtccggcga gaacttgccg tatcgggcat cgatgttgcg
    12241 gatttggttt tggaaaacgg ttcgggtctg tccagaaaag aaagggtaac ggcgagaatg
    12301 atggcgcaaa tgttggaaac ggcttatttc agcccgtttg cacaagattt catcgacacg
    12361 ctgcccatcg ccggcacaga cgggacttta cgcaaccgct tcaaacaaag cggcgggctg
    12421 ttgcgcttaa aaaccggcac gctcaacaat gtccgcgccc ttgcaggtta ttggctgggc
    12481 gacaaaccga tggcggtggt cgtcatcatc aacagcggcc gcgccgtttc cctgctgccc
    12541 gacttggaca acttcgttgc caaaaacatc atctccggcg gcgacggctg gctggatgcg
    12601 aaactgatgt gcaaagaacg ccgcgcctga atcgggaaaa tataaaaatg ccgtctgaac
    12661 tgttcggacg gcatttttga ttttcaaacc ggaattacag ccccgctgcc gctttcaatg
    12721 aggccgcttt gtcggtgcgc tcccaagtaa actcaggttc ttcgcggccg aaatgtccgt
    12781 aggcggcaga ttttccgtaa atcgggcgca agaggtcgag catttggacg atgcctttgg
    12841 gacgcaggtc gaaatgttcg caaaccagcg caatcagttt ttcctcgctg attttacccg
    12901 taccgaaagt atcgatggaa atcgaagtcg gttcggcaac gccgatggcg taggaaactt
    12961 ggatttggca ttgggttgcc aagcctgcgg cgacgatgtt tttcgccaca taacggcagg
    13021 cgtaagcggc ggaacggtcc actttggacg ggtctttgcc ggagaatgcg ccgccgccgt
    13081 gcggagccgc gccgccgtag gtatcgacaa tgattttgcg gccggtcaaa ccgcagtcgc
    13141 cttgcgggcc gccgatgacg aagcggccgg tcgggttgat caggtatttt gtttcgtcgg
    13201 tcagcagttc gggcggcaaa acgggcttga taatctgctc aatcacggct ttactcagtt
    13261 cttcatggct gatggcagga tcgtgctggg tggacaggac gacggtatca atgcgtttca
    13321 ccttgccggt ttcgctgtcg taaaccacgg tcagttgggc ttttgcgtca gggcgcagcc
    13381 aaggcaggcg gccgtctttg cgcaattcgc tttgacgctg catcaggcgg tggctgtaat
    13441 agatggcaaa cggcatcagg gtcggggttt cgtcgcaggc atagccgaac atcaagcctt
    13501 ggtcgcccgc gccttggttc aggtcgatgc cttcgccttc gttcacgcct tgggcgatgt
    13561 cgggggattg ttggtcgtag tacacgccga ccgcgcagcc gttggcgtca aagcccagct
    13621 cggaggagtt gtagccgatg cgtttgatgg tttcgcgtgc gactttgatg tagtccactt
    13681 gggcggtggt ggtgatttcg cccgccaata cgcacaagcc tgtgttgacc aaagtttccg
    13741 ccgcgacacg cgctttgggg tcttgcgcca agatggcatc caaaatcgca tcggatactt
    13801 ggtcggctac tttgtccgga tggccttcgg aaaccgattc ggaagtaaac agatattcgc
    13861 tcattgaaat tttccttgaa aaacaaacca tcttcttcag acagcatgtt gtatgaacat
    13921 aatgtcgaca gcgggaaata tagcaaaatt tccctattcg tgccattcag ttgagaaata
    13981 ttcctattta aacggcgctt tggaatctct gtccgtacgt ttcgtacagg caaaaaaatt
    14041 cccgcatcaa gcgggtttgg attgctccgg cgagccacat cggtttttca accgtccacc
    14101 ttacctttcc gtttgaaaag caggttggca tggaattccc aactcttaat gcagcgcgca
    14161 tcgtagcaga aaaggcatat tgccgcaata cttccctttt tcagacggca tgtttcgttt
    14221 acaattcagg ctgtttcccc ctttgcgaac cgccatgcac atcctgttga ccgccctgct
    14281 caaatgcctc tccctgctgt cgctttcctg tctgcacacg ctgggaaacc ggctcggaca
    14341 tctggcgttt taccttttaa aggaagaccg cgcgcgcatc gtcgccaata tgcggcaggc
    14401 gggtttgaac cccgacacgc agacggtcaa agccgttttt gcggaaacgg caaaatgcgg
    14461 tttggaactt gcccccgcgt ttttcaaaaa accggaagac atcgaaacaa tgttcaaagc
    14521 ggtacacggc tgggaacacg tgcagcaggc tttggacaag ggcgaagggc tgctgttcat
    14581 cacgccgcac atcggcagct acgatttggg cggacgctac atcagccagc agcttccgtt
    14641 ccacctgacc gccatgtaca agccgccgaa aatcaaagcg atagacaaaa tcatgcaggc
    14701 gggcagggtg cgcggcaaag gcaaaaccgc gcccaccggc atacaagggg tcaaacaaat
    14761 catcaaggcc ctgcgcgcgg gcgaggcaac catcatcctg cccgaccacg tcccttctcc
    14821 gcaggaaggc ggcggcgtgt gggcggattt tttcggcaaa cctgcataca ccatgacact
    14881 ggcggcaaaa ttggcacacg tcaaaggcgt gaaaaccctg tttttctgct gcgaacgcct
    14941 gcccgacgga caaggcttcg tgttgcacat ccgccccgtc caaggggaat tgaacggcaa
    15001 caaagcccac gatgccgccg tgttcaaccg caataccgaa tattggatac gccgttttcc
    15061 gacgcagtat ctgtttatgt acaaccgcta taaaacgccg taacgaaaat aaaaatgccg
    15121 tctgaacagg tttcagacgg catcttgtca tttgacgatt tcggacaacg accaacgcgg
    15181 acggacgttg aacgcaccga cctccctgcc cttgcccagg tgcatcgcac cggcaaaggc
    15241 aatcatggca ccgttgtccg tgcagtatgc catcggcggg aaaaacacgc tgactttttc
    15301 ggacggatgt ttcggcttgc ctttgggggt cgggatttgt accgtcatgt tgccgaaagt
    15361 ttcacggagt ttgcggtttg caccgacccc gccggcgacc actacggttc tgaaccctgt
    15421 ctgcaacagg gcttttttca cttttgcctc caacacttct actaccgcat cttgaaacgc
    15481 acggcaaata ttgttgcggg tttgctcggg tatggtttcc gaaccgtttg cctcacgcac
    15541 tttttcaacg gcagtcaaaa cagcggtttt caatcccgaa aaactcatct gcaaatcgtc
    15601 ggaatgaatc atcgggcgcg gaaaaacaaa cgcttcgggc ctgcccgatt ccgcaagttc
    15661 cgacagtttc gcaccgcccg gatacggcaa acccagcagt ttcgccgttt tgtcgaatgc
    15721 ctcgcccgcc gcatcatcga cgctctcgcc caaaagctcg taatcgccta tgcccctgac
    15781 cgccataatc tgcgtatgcc cgcccgaaac caacagcgcg acaaaaggaa agtcgggttt
    15841 ttcctccgcc aacagcggcg acagcagatg tccttccaaa tgatggacgg ggatgacggg
    15901 cttatccaac gctaaagcca gcgcgttggc gtagctcgaa cccgccagca gcgcaccgcc
    15961 caaaccgggc ccctgcgtaa aggcaaccgc gtcaatatcg ccatacgatg cgcctgcctg
    16021 cgccaaacag ccttcagtca acggaacaag gcggcggata tggtcgcggc tcgccaattc
    16081 cggcacgacc ccgccgtatt ccgcgtgcat tgccatttga gtgtgcaggc agtgcgaccg
    16141 caatcctcgt tcggtgtcgt acagcgcaac gccggtttcg tcgcaagaag attcgattcc
    16201 taataccaac atggtctgat gccgttaaaa actgaaaaac gtattctagc gaatttcggc
    16261 acgactgccg tatccccaaa acggaacatg ccgtctgaag cccttcagac ggcatcgtcg
    16321 caccgtatca aagcgttccg taagaatgca gcccgctcaa aaacatattc acgccgataa
    16381 aggcgaatgc ggttacgaac aaaccgataa tcgcccacca cgccagcact ttgccgcgcc
    16441 agcccgccac cagccgcaaa tgcagccaca cggcgtaatt caaccaaacg atgaacgccc
    16501 aagtctcttt cggatcccag ctccaatagc gtccccaagc gtccgccgcc cacaacgcgc
    16561 ccaaaacggt ggcaatggta aagaacaaaa agccgacggc aatcgcctta tacatcacct
    16621 cgtcgatcaa tgccgacggc ggcagccaca gttttccgcc tttttcttcc gcacgcaggg
    16681 aaaccagttc ggcaataccg agcatcgcgg aaatgcagaa cgcgccgtaa ccgataaagt
    16741 ttgccggaac gtggattttc atccaccagg actggagcgc gggaatcagc ggctggatgg
    16801 tatgcgcctc acgcgacacg ctgtaccaca acacaaagcc gaccacgacc gccataaagc
    16861 cgaacacgaa gccgcccaat ttctgcacgg caaatttacc ctcgtaatac aaatacatca
    16921 gcgcggtaat gaccaaaaac agaatgaaca cttcataaag gttggacacg gggatatgcc
    16981 ctgcatcggg acggagcaga tagctttcgt gccaacgtac cagcagaccg gtaaagcctg
    17041 ctacggcaga cacccatgca aacacggttc ccatacccaa cagcgtgttg gtcggcacat
    17101 ttttcacgct tgccaaaacc gcgcccgaaa tataggcgaa cagggcgaag aagacaaaag
    17161 cacactgcca catgatcgcc gattggctgc tgaggaaata ccgcaacagg aaaatctctg
    17221 ccgatttaat gttgccgcca tacagaccga cggcggcata ggcaagcaat acgcttaaag
    17281 gaacaaacca gcgcatcggt ttgaaaaacc aacccaaaaa cacggcaata ccggcactcg
    17341 cccacaacat gaccgtttcg taaatgtcca tatgcatacc ggaacgggtc tgcacgaaaa
    17401 ccgtagccgc aaaaaccagc acggcaaata cccaatccca aagattcaga ttgcggatca
    17461 aagatttctg aatcagcagc tcgtgttccg gaagggtttt atagtgttca gtcatgattc
    17521 aagtccttgc cgagccgttg caggctctcg acgtgttttg gaaattcctt ctgcaaatcc
    17581 cgttcgctgc gggccgaaga catagcaaaa cggattttgc cgtctgaaaa caatacccac
    17641 gcccgttttt cgcgcacata aaacataaat accgtaccca aaaccaacaa taccgagccg
    17701 agatagacca aaagcgcacc cggcgaacgg gtcatctgca aacctgagga acgcacctcg
    17761 gaaaacccgt caagctggag cagcataggc gcgggatatt ccgtcagccc cgtataggca
    17821 tccatactgt gcagcaggaa acggttccgc gcttcatcct gccgccattc gggcaagccg
    17881 taccggcgta tggtttcatc caaagcagcg ttcatcacgc cgtaaagcat ttcgtagaaa
    17941 tagccctgca tcttatcctg ctgccctttc gggatattgg acgtaataaa ttcgtccaat
    18001 cccaaatagc ctttttgcgc aaagatattc agcgtgtttt ccgcagccag catgaattgt
    18061 tcgcggattt cggcaggtgc gtctttggtt gcgtcggcaa ccagacgttt gcgcccttcc
    18121 ccatctttca aaaactcacg caatgccata aaggtgtccg ctttcaactg cttgtccaag
    18181 gggatacgca gccagcggta ttgctgctgc aagccgctgc gcgtgccggt cagccaaaaa
    18241 taatctttgt cctgcaaaat cggcagcata tagtttttat attcgaccgc ctgccctgcc
    18301 gcatcacgga tgcggtacac gatggaaggg ccgatattgg tgtatttttt accttcctga
    18361 gtaacggcgc ggacatcgtt cagagtggat ttcaggcttt tttcccgttc cgcaccctcg
    18421 ctcatgtcct ccacattcat agaagtgaac tgatcgaact caagacgata tttgtgtttg
    18481 ccgatttcca acggaaactg gtgtatggag gttgccttca acacgacagg ttcgcgcgaa
    18541 gcatccctca aattccacgc cttgaatgtc aaatccgaac cgccgtcggc aaaactcgcc
    18601 tgataaatcg tgatgccgtg caaggtcaaa ggatggttca cgcggatggt gcgctcgagt
    18661 ttctcaccgg ttcccttgtc cgttacttca atatcgctgg caaaatcgcg cggcataccc
    18721 gtattgtaaa aatcgatatg gaattttttc agtttgactt caaaaggcaa gtcctgaacc
    18781 aacatcccgt tgtcggcatt caggaaaacc acatccgcac tttgcccctc ggaaatattg
    18841 acgttgcccc taaatgagag attggacgca cccaaaatac tttcgggctt gaaatccttg
    18901 gcataaaccg cctgattgtc cggaacaatc cgaccggcca gcatacccag cttcagcagc
    18961 aggttactgt ctatcaaccc gcccaggcaa atgacaatca aagcaacatg ggcaaagata
    19021 tagccccatt tgttcatcgt gccttttttg gcggcaatca gaaccgaccc gtcctcacgg
    19081 ctgacggttt ttccctgaaa accccgcacc tccagataac gtttggcaac ttcgggggca
    19141 atttttacat ccaacagcga agaatggcgc atcgccgcca gagatttttc tttaaccttt
    19201 tcccggaaag acttcatttc gcgccaaaac ggcggaacgt tacggattaa acacaaactg
    19261 gtagaaacca ccagaaacat catgataacg acaaaccatg ccgaagcata gacatcgtac
    19321 aaacccaaaa aatcaaaaat ccgagtccaa aacggtccga atttgaccaa ataatccgtc
    19381 tgcggctggt tttgctgtaa caccgtgccg ataaccgatg caatacccag cagactgagc
    19441 aaagcgaccg caaagcgcat ggagctgaaa aaagcgaacc acggacggga aagaagtgtg
    19501 ggggatatac gggatttact cattgtgtgt ttattccgcc atcaggaata tgggaaagca
    19561 gaattgggca gacagaaaac aacgtcccga ttctactgtc ttgatgcttt ttatttcaag
    19621 acaatgaaga cagcctgcat cgattccaac ggttgcgatt gaaaaaactt atcgcaaaat
    19681 tttcagaagc cgtctgaaaa ccttttcaga cggcttctga agcagaccct tggatgatta
    19741 acgcaaaccc tggataaagt tagcaactgc ttttaaatct tcttcggaca tacggtttgc
    19801 aatatcttcc atgatggtat ttttacgctg accggacttg taggcattca tctgttcaac
    19861 aatatatgcc tggtgctggc cgcccaaacg cggataagcc tgaatttcgc ttccgcctcc
    19921 cggcataccc gcaccgctcg gaccgtggca ggacatacac gccggcactt ttttatcgct
    19981 caaaccgccg cgatagattt tcgcgcccaa ttcgggattt tccttaggat tggcttcgcc
    20041 ggatttgggc tgctgtttgg catagaatgc ggatacgttc aaaatatcct gatcgctcaa
    20101 attcattacc accggtttca tcacagctgc cgaaccgtgg gtgcgtttgc cgtcgcggat
    20161 gccgatagtt tgatgataga tgtaagcagt atgctgtgcc gccaaacgcg gatacatcgc
    20221 aatgccgctg ttaccgtctg ctgcatggca agccgcacaa accgttgcgg caacctgttt
    20281 gcctttttcc acgtctgctt tgggagatgc ggaaaccgca ccggcagcca aaacaaaggc
    20341 caataaagtc aatcgtctca tggagtgctc ctgattacag cattggataa cgcaacaatg
    20401 ctctttttta tattcaaata tgggattttt gacccgatta aaaccgatga ttctgtaaac
    20461 gtgttattct atactaaatt tacattaaat taccactgtg tttcacataa aaccaaccgc
    20521 atatttttgc tgtcggacaa acggcggcgg aaaacaagga tatgcccatg aacctttttc
    20581 aaaacgccaa attcttcacg acggtaaacc atctcaaaga cctgccggac acaccgctcg
    20641 aaattgcctt tgtcggcagg agcaatgccg gaaaatccag tgccatcaat accctgacca
    20701 accatgtccg tcttgcctac gtttcaaaaa caccgggacg gacacagcat atcaacttct
    20761 tcgagctgca gaacggcaat tttatggtcg atttgcccgg ctacggttat gcccaagtcc
    20821 ccgaagcggt acgcgcacat tgggtcaatc tgctcggcga ctacctccgc catcggaaac
    20881 agcttatcgg gctggttttg attatggatg cccgccatcc tttaaaagaa ctcgacatcc
    20941 gtatgctgga ctttttccac acgaccggca gaccggttca catcctgctg tcaaaagccg
    21001 acaaattatc caaaaacgaa cagataaaaa ccctgtccca agtcaaaaaa ctgctcaaac
    21061 cttattccga caggcaaaac atcagcgtac agctgttttc cagcctgaaa aaacaaggta
    21121 ttgacgaggc caaccgaact gtcggaagct ggtttgacgc agcagatgcc gccgcttcct
    21181 ctccagagga aaactgacct caattatacg gaaaccgtat tccccccact tgaccggccg
    21241 caaacattta aaaaattgcc actgccaaat ctaaaatgcc gtccgaaaga cttttcagac
    21301 ggcattttgc ggagtcttta aaacagggaa tccaactgct gctgtttgga atccgtattg
    21361 ctcggaagca ccggcgtttc ctgtacgtcc tgacgcgttt cgtcagacct tccctgctgt
    21421 tcgttttcta ctgccgcttc atcatcttct tttgcccgtc gggaaggttg cggcgcaata
    21481 ccgctgttgt ccagcatcaa gcccggatcg gttaccatac gttccttcat atagtattcg
    21541 ccattgctgc tgaccacacc ttcaggcatt ttcatccctt tgccctgctt tcctttcaac
    21601 gcaaaacgca tatagtccac ccaaaccggc accgcaatcg taccgccgta gccggcacgc
    21661 cccatactct taggtttgtc gaagccgata tatacggcag taaccacatc agggttaaaa
    21721 ccgacaaacc acgcatcttt attgtcgttg gtcgtacccg ttttaccggc aatatccgtt
    21781 cttcccaacg cagctgcccc ccttgccgta ccgacacgga ccacatcctg cataatctta
    21841 tacataatat aggcattgcg cggatcgatt gcctgaggcg cattttgccc tgccaccaaa
    21901 ggttgcattt gggcgcgcaa cctgccgtct ctgtcataaa tcttatcgat cacgtgcgaa
    21961 gaaaccctat atccgccgtt cgcaaataca ctatatgcct ccgccacttt caacggcgtc
    22021 gtctcgcccg tacctaaagc catagacagg cttgccggca gctcggacgg cctgaagccg
    22081 aaacgccgga tatactgttg cgcgtaaccg acaccgatag acatcaaaat acggatggaa
    22141 accatattct tggaagccgt cagagcctgt ctcaaagtaa tgtagccgga atatctgccg
    22201 tctgaatttt taggtgtcca aaccgaaccg ttcggccctt tccccggcag ggaaatcggc
    22261 gcatcgttaa ccactgtgga cgcggtcatc cccttagata atgccgccga atagacaaac
    22321 ggcttaaagg tcgaacccgg ctgccgcatt gcctgaacgg cacgattgaa tgttttgctg
    22381 tgaaaatcat aaccgccgac cagcgcgcgc acagctccgg tttttgcatc cagcgaaacc
    22441 aaagccccct gcggcaacgg ctcttgaacc accgcccaac gcccgccgtt gtttttgaca
    22501 cggatgaccg cgcccctgcg gatacggtcc tcccccattt tctcattatc gaccgctcgg
    22561 gccgcaaaac ccaaggcgcg cctgtcaagc gcaacccgcc tgccgccggg cagctgtatg
    22621 acgacatttt tctttttagt aacatccaac acaacggcgg gaaccatttt atcgacggta
    22681 tagagtcccg acagatactg gctgacagtc tcctcgacat cttcactctt actcaaatcg
    22741 atatagtttt ccgcaccgcg gtagctgctg ccgcgatcga aattccgtag agccttgcgc
    22801 aatgcctcgg ttgccgcctt ctgatgatcg gtgcggaccg tggtataaac cttaaaaccc
    22861 tgcgtatagg catcttcacc atatttctca tacagttccc gacgcaccat ttccgccaca
    22921 tataaagcac tctgatcgat tttccgaaca aaccgctcat aatgcagttc ctcattcaat
    22981 gcctgatcgc gctgttgcac ggtaatcatc ttctcctcga gcatattgtt caaaatatac
    23041 ttctggcgca acttggcacg ctccggatta acaatcggat tataggcaga cggagccttg
    23101 ggcagtcccg caagcatggc ggcttccgcc aaagtcaaat ctcggacatt cttattgaaa
    23161 tagatttgcg cggcagatgc aaaaccatag gcgcgctgac cgaggtaaat ctgattgaaa
    23221 tacaactcaa ggattttgtc tttgcttaaa gactgctcga ttttataggc aagcaacacc
    23281 tcattgaatt tgcgtgtgaa cgttttttca ctgctcaaat aaaaattttt cgccacctgc
    23341 tgtgtaatcg tactcgcacc cgactgcacg ctgccggaca cgacattgcc gacggcagcg
    23401 cgggcaacac cccaaacatc caccccccaa tgccggtaaa agcgtttatc ctcggcggca
    23461 ataaccgcat tccgcaacac ctcggggaaa tcgccgattt ttgtaaattc gcgccgctgc
    23521 tccccataca taccgatgac ttctccatcc gccgaataaa tagtcaacgg cattttaggc
    23581 tggtaatgct gcaaagaatc caaagacggc agtttcggat acgttaccaa aatagcaatg
    23641 gcaaccagac ccactccaaa tacacaaaaa ccaaaaaaca aaccaaaaca agtagttaaa
    23701 atctttttaa tcatagctga ataataattt accattattg gtattaaata aagtaaaata
    23761 gcaaccgatt tctacaaagc acggtttcaa tgtgcaaaga acaaggaatc cattacggat
    23821 accgaaacgg ttactcactg tacaaataaa gcaggaactt tcatcatgcg cttgtttaaa
    23881 agcttgaaaa accctaaaaa aacagatgcc aagctcccta aaaaatcttc gggactcaat
    23941 aaccgcgcgg caatcggcat cgatatcgac cagcattcca tcaaaatggt acaattgtca
    24001 ggacgtagtt taaaccaaat tcaattggaa aaatacgtca ttgccaaatt accaaagaat
    24061 atcattcaag gcaataaagt ccaaaattac gatcaacttg ttacatattt gcaacaagcc
    24121 tatgccaaac tgggtacttc gtgcaaaaac atcgtcgcgt ccgtcccgca aaatctggca
    24181 accatcgaac aattgaccta cacggccaaa gatgcagaat tggacctgca ggggttcgtg
    24241 gagtcctcca tctccgaagc cagctcgata tcgctcgaag aagccaatta cgactatcag
    24301 gtcttgtccc aatcggccgt cggcgaagct gttttgtccg tcgcatcgag aaaggatgaa
    24361 atcgaacccc tgattgacgc attcaacgca gccggtatga aattatccgc gcttgatgtg
    24421 gacattttcg gacaatacaa cgcctacgcg ctgtggataa accattttgc ccccgagctt
    24481 gcggacgaaa aagtggccat tttcggcgta tatgccgcac agacctacgc cctggtcatc
    24541 caagacggaa aaatcctata caaacaggaa acctccgtca gcgcagaaca gctcaaccaa
    24601 ctcatccagc gcacctatca ggtaacagca gaaaaagcgg aagagatcat caactccccg
    24661 caaaaacctt ccgattacca agaaagcgtg gcaaactatt tcaaccagca gattacccaa
    24721 gaaatacaaa gggtcttgca gttttattac accacgcaga ccgcagacga tatgaccgac
    24781 atcaagcata tcctgctgac gggggaagcg gtgcgccaga aaggcatcgc ccaaaccgtc
    24841 gcctcgcaaa ccaatgcgga tgtacaatgc gtccatcccg cgcgttattt tgcgaacgac
    24901 ctcaaaacag acgaacaaca attcgaactt gatgcgccga cactgaccaa ggcgttcggt
    24961 ttggcggtac ggggattata attatgaaca atttaatcaa aatcaacctc ctcccctaca
    25021 gggaagagat gaacaaacgc aaacagcagc agtttaaaac gctgatgtac ggcgccgtgc
    25081 tgacgggcgt tgccgccgtt gcggcaacct acctgtttat cgacaatatg atcaataaac
    25141 agtcggaaaa aaacacgctg ctggaaatct ccatcgcaca cttggatacc gagctgtcgg
    25201 aaatacaaaa gctcaaacag gaaaaagatg ccttcctgat taagaaaaac aaaatcgggg
    25261 agctccagat caaacgcctc caagccgcaa aaatcctcga cagcctgaat gaggccgtcc
    25321 ccggaagcac ctacctaacc tcgctggatg ccgttaccgc cgactcttat cggctcagcg
    25381 gcaggacatc cagcgacaac cgcgttgccg ccatgatgag ggcgatgccc aataccggca
    25441 tattcaagca acccgaattg ttaagcatca agaaaaacaa ttcgcatcaa gaatttaccc
    25501 ttcaggcaac attacaacct atcgtaaagg cggccgaatc caaagagaat ccggcttcgg
    25561 gaaacgcaca ggaggcaaac tgaatggctt ctaaatcatc taaaaccaac ttggatctca
    25621 acaaccttca cctgctcaac cttcctgcca ggctttttat cgccctgctg gtcgttgccg
    25681 ccgtgctggg gctcggttat gccggattgt tcaaaagcca gatggaatcc cttgaggaat
    25741 atgaagcaaa agaaaccgaa ctgaaaaaca cctacaaaca gaaaagtatc gacgcggcca
    25801 gcctgaacaa ccttagggac gaacttgcct caatccgctc tgccttcgat atcatgttga
    25861 aacagctgcc gacagatgca gaaattccca atttggttca agagcttcat caggcgggtt
    25921 cgagcaacgg tctgcgcttg gacagcgtta tgccccaacc tcccgtagat gacggtccca
    25981 tcaaaaaatt accctattcc atttccatta ccggaaatta cgaacagatc agccaattta
    26041 cccgcgatgt cggcagtctc tcccgaatca ttacccttga gtcgctgaaa attgcccaat
    26101 ctccggaaaa cggcggcaat cctgacggca agagcagtat cctgaacctc agcgccattg
    26161 ccaccaccta ccaagcaaaa tccgtagaag agcttgccgc agaagcggca caaaatgccg
    26221 agcaaaaata acttacgtta gggaaaccat gaaacactat gccttactca tcagctttct
    26281 ggctctctcc gcgtgttccc aaagttctga agacctaaac gaatggatgg cacaaacgcg
    26341 acgcgaagcc aaagcagaaa tcataccttt ccaagcacct accctgccgg ttgcgccggt
    26401 atacagcccg ccgcagctta cagggccgaa cgcattcgac ttccgccgca tggaaaccgc
    26461 caaaaaaggg gaaaatgccc ccgacaccaa gcgtattaaa gaaacgctgg aaaaattcag
    26521 tttggaaaat atgcgttatg tcggcatttt gaagtccgga cagaaagtct ccggcttcat
    26581 cgaggctgaa ggttatgtct acactgtcgg tgtcggcaac tatttgggac aaaactacgg
    26641 tagaatcgaa agcattaccg acgacagcat catcctgaac gagctgatag aagacagcac
    26701 gggcaactgg gtttcccgta aagcagaact gctgttgaat tcttccgaca aaaacaccga
    26761 acaagcggca gcacctgtcg cagaacaaaa ttaagaagag gattactcca ttatgaatac
    26821 caaactgaca aaaatcattt ccggtctctt tgtcgcaacc gccgcctttc agacggcatc
    26881 ggcaggaaac attacagaca tcaaagtttc ctccctgccc aacaaacaga aaatcgtcaa
    26941 agtcagcttt gacaaagaga ttgtcaaccc gaccggcttc gtaacctcct caccggcccg
    27001 catcgccttg gactttgaac aaaccggcat ttccatggat caacaggtac tcgaatatgc
    27061 cgatcctctg ttgagcaaaa tcagtgccgc acaaaacagc agccgtgcgc gtctggttct
    27121 gaatttgaac aaaccgggcc aatacaatac cgaagtacgc gggaacaaag tttggatatt
    27181 cattaacgaa tcggacgata ccgtgtccgc ccccgcccgc ccagccgtaa aagccgcgcc
    27241 tgccgcaccg gcaaaacaac aggctgccgc accgtttacc gagtccgtag tatccgtatc
    27301 cgcaccgttc agcccggcaa aacaacaggc agcggcatcg gcaaaacaac aggcggcgac
    27361 accggcaaaa caaaccaata tcgatttccg caaagacggc aaaaatgccg gcattatcga
    27421 attggcggca ttgggctttg ccgggcagcc cgacatcagc caacagcacg accacatcat
    27481 cgttacgctg aaaaaccata ccctgccgac cgcgctccaa cgcagtttgg atgtggcaga
    27541 cttcaaaaca ccggttcaaa aggttacgct gaaacgcctc aataacgaca cccagctgat
    27601 tatcacaaca accggcaact gggaactcgt caacaaatcc gccgcgcccg gatactttac
    27661 cttccaagtc ctgccgaaaa aacaaaacct cgagtcaggc ggcgtgaaca atgcgcccaa
    27721 aaccttcaca ggccggaaaa tctcccttga cttccaagat gtcgaaatcc gcaccatcct
    27781 gcagattttg gcaaaagaat ccgggatgaa cattgttgcc agcgactccg tcaacggcaa
    27841 aatgaccctc tccctcaaag atgtgccttg ggatcaggct ttggatttgg ttatgcaggc
    27901 gcgcaacctc gatatgcgcc agcaagggaa catcgtcaac atcgcgcccc gcgacgagct
    27961 gcttgccaaa gacaaagcct tcttacaagc ggaaaaagac attgccgatc tgggcgcgct
    28021 gtattcccaa aacttccaat tgaaatacaa aaatgtggaa gaattccgca gcatcctgcg
    28081 tttggacaat gccgacacga ccggaaaccg caacacgctt gtcagcggca ggggcagcgt
    28141 gctgatcgat cccgccacca acaccctgat tgttaccgat acccgcagcg tcatcgaaaa
    28201 attccgcaaa ctgattgacg aattggacgt acccgcgcaa caagtgatga ttgaggcgcg
    28261 tatcgtcgaa gcggcagacg gcttctcgcg cgatttgggc gttaagttcg gcgcgacagg
    28321 caggaaaaaa ctgaaaaatg agacgagcgc attcggctgg ggcgtgaact ccggcttcgg
    28381 gggcggcaat aaatgggagg cccaaaccaa aatcaacctg ccggttgccg ctgccgcaaa
    28441 cagcatttcg ctggtgcgcg cgatttcctc cggcgcgttg aatttggaat tgtccgcatc
    28501 cgagtcgctt tcaaaaacca aaacgcttgc caatccgcgc gtgctgaccc aaaaccgcaa
    28561 agaggccaaa atcgaatccg gttacgaaat tccttttacc gtaactacag cctcgggcgg
    28621 cggcaactct accaacacgg aactcaaaaa agccgtcttg gggctgaccg ttacgccgaa
    28681 catcacgccc gacggacaaa tcatcatgac cgtcaaaatc aacaaagact cgcctgcaca
    28741 atgtgcttca ggcagcaaca caatcctatg tatttcgacc aaaagcctga atacgcaggc
    28801 tatggttgaa aacggcggca ctttgattgt cggcggtatt tatgaagaaa acaacggcaa
    28861 tacgctgacc aaagtccccc tgttgggcga catccccgtt atcggcaacc tctttaaaac
    28921 acgcgggaaa aaaaccgacc gccgcgaact gctgattttc attaccccga ggattataga
    28981 tacggccggc aacagcctgc gctattgatg cgtcaaaata agggcatatg ttttacagca
    29041 tatgcccttt ctttatgctt tttgccgcga ccgaaatgcc gtcattcccg cgcaggcggg
    29101 aatccaactt gtccggtttc ggttgttttt cgtctcgtaa cttttgagcc gtcattcccg
    29161 cgaaatcgga aatccagtcc gttcagtttc ggtcatttcc gataaattcc tgttgctttt
    29221 catttctaga ttcccacttt cgtgggaata acggcggaag ggataaatcc tcgcaatcca
    29281 aagcctcgtc atttccacaa aaaacagcaa cccgaaacac cccgtcattc ccgagcaggc
    29341 gggaatctag gtctttaaac ttcggttttt ttccgataaa ttcttgcctc attgaaattc
    29401 tagattcccg ctttcgcggg aatgacggga tatgggtttc cgtgcggacg tgttcggatt
    29461 tccgcctgcg cgggaatgac gattcgggta ttactgacga ttcatcgggc cgtagggtgg
    29521 gcttcagccc actaatttca ccgcatcaag ggttttgggg aataggcgta actcggcggt
    29581 ttcggttttt ggcgaagttg gttggaatcg gcgggctgaa gcacacccta cagtccaccc
    29641 tacaacccat cttacgcatc cgccttacgc atttatcctt cgcctgccga tatgccgtca
    29701 ttcccgcaca ggcgggaatc tagaaccgca acgcggcagg aatct
//
LOCUS       DAWTHD010000012        29548 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWTHD010000012 DAWTHD010000000
VERSION     DAWTHD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.012"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(255..596)
                     /locus_tag="WM704_000424"
     CDS             complement(255..596)
                     /locus_tag="WM704_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFA9339922.1"
                     /translation="MTDTDTQADRFEQMMRQAVDKLFEQHDGKLESMDGREQELVLIW
                     RAEADIGNGGILQFVCNWGLPAAEKTCSVLKKIGAVHSAMLIHRAADALGKEIRHLQS
                     EGKNLKEMWDI"
     gene            complement(654..2030)
                     /gene="argH"
                     /locus_tag="WM704_000425"
     CDS             complement(654..2030)
                     /gene="argH"
                     /locus_tag="WM704_000425"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFA9339923.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHLLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(2049..2918)
                     /gene="galU"
                     /locus_tag="WM704_000426"
     CDS             complement(2049..2918)
                     /gene="galU"
                     /locus_tag="WM704_000426"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFA9339924.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(2946..3545)
                     /gene="rdgB"
                     /locus_tag="WM704_000427"
     CDS             complement(2946..3545)
                     /gene="rdgB"
                     /locus_tag="WM704_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFA9339925.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(3538..3771)
                     /locus_tag="WM704_000428"
     CDS             complement(3538..3771)
                     /locus_tag="WM704_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339926.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(3879..4412)
                     /locus_tag="WM704_000429"
     CDS             complement(3879..4412)
                     /locus_tag="WM704_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFA9339927.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(4514..4972)
                     /gene="nudB"
                     /locus_tag="WM704_000430"
     CDS             complement(4514..4972)
                     /gene="nudB"
                     /locus_tag="WM704_000430"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFA9339928.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            5183..5259
                     /locus_tag="WM704_000431"
     tRNA            5183..5259
                     /locus_tag="WM704_000431"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5217..5219,aa:Pro,seq:ggg)
     gene            5522..7408
                     /gene="mafB"
                     /locus_tag="WM704_000432"
     CDS             5522..7408
                     /gene="mafB"
                     /locus_tag="WM704_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFA9339929.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
     gene            7439..7858
                     /locus_tag="WM704_000433"
     CDS             7439..7858
                     /locus_tag="WM704_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339930.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            7936..8019
                     /locus_tag="WM704_000434"
                     /pseudo
     CDS             7936..8019
                     /locus_tag="WM704_000434"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8016..8258
                     /locus_tag="WM704_000435"
     CDS             8016..8258
                     /locus_tag="WM704_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HFA9339931.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            8336..8581
                     /locus_tag="WM704_000436"
     CDS             8336..8581
                     /locus_tag="WM704_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9339932.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLIREGLHHDTGHIGWRAMSKGK"
     gene            8584..8974
                     /locus_tag="WM704_000437"
                     /pseudo
     CDS             8584..8974
                     /locus_tag="WM704_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9014..10471)
                     /locus_tag="WM704_000438"
     CDS             complement(9014..10471)
                     /locus_tag="WM704_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFA9339933.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(10486..11316)
                     /locus_tag="WM704_000439"
     CDS             complement(10486..11316)
                     /locus_tag="WM704_000439"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFA9339934.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(11393..11782)
                     /locus_tag="WM704_000440"
     CDS             complement(11393..11782)
                     /locus_tag="WM704_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFA9339935.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(11929..12456)
                     /gene="nspA"
                     /locus_tag="WM704_000441"
     CDS             complement(11929..12456)
                     /gene="nspA"
                     /locus_tag="WM704_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFA9339936.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYVGKVNTVK
                     NVRSGELSAGVRVKF"
     gene            complement(12899..14074)
                     /locus_tag="WM704_000442"
     CDS             complement(12899..14074)
                     /locus_tag="WM704_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HFA9339937.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(14131..16602)
                     /gene="ligA"
                     /locus_tag="WM704_000443"
     CDS             complement(14131..16602)
                     /gene="ligA"
                     /locus_tag="WM704_000443"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFA9339938.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     ANIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(16736..18022)
                     /locus_tag="WM704_000444"
     CDS             complement(16736..18022)
                     /locus_tag="WM704_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFA9339939.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(18216..18788)
                     /gene="ampD"
                     /locus_tag="WM704_000445"
     CDS             complement(18216..18788)
                     /gene="ampD"
                     /locus_tag="WM704_000445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFA9339940.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            18872..19867
                     /gene="mltG"
                     /locus_tag="WM704_000446"
     CDS             18872..19867
                     /gene="mltG"
                     /locus_tag="WM704_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFA9339941.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            19927..20547
                     /locus_tag="WM704_000447"
     CDS             19927..20547
                     /locus_tag="WM704_000447"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFA9339942.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            20769..22049
                     /locus_tag="WM704_000448"
     CDS             20769..22049
                     /locus_tag="WM704_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HFA9339943.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            22451..23482
                     /locus_tag="WM704_000449"
     CDS             22451..23482
                     /locus_tag="WM704_000449"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFA9339944.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKGKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            23688..24221
                     /locus_tag="WM704_000450"
     CDS             23688..24221
                     /locus_tag="WM704_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFA9339945.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            24206..24313
                     /locus_tag="WM704_000451"
                     /pseudo
     CDS             24206..24313
                     /locus_tag="WM704_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            24443..24625
                     /locus_tag="WM704_000452"
     CDS             24443..24625
                     /locus_tag="WM704_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFA9339946.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            24622..25383
                     /gene="kdsB"
                     /locus_tag="WM704_000453"
     CDS             24622..25383
                     /gene="kdsB"
                     /locus_tag="WM704_000453"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFA9339947.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            25406..25702
                     /locus_tag="WM704_000454"
     CDS             25406..25702
                     /locus_tag="WM704_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFA9339948.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EVSGEFWFRMAETLSTLPPNRSLDLRMNGGRLSTAVSILSVMIEDNPDIPQLWA"
     gene            25724..25921
                     /locus_tag="WM704_000455"
     CDS             25724..25921
                     /locus_tag="WM704_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339949.1"
                     /translation="MVHGHKARADGLVQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            26078..26170
                     /locus_tag="WM704_000456"
     tRNA            26078..26170
                     /locus_tag="WM704_000456"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26112..26114,aa:Ser,seq:gct)
     gene            26444..27229
                     /locus_tag="WM704_000457"
     CDS             26444..27229
                     /locus_tag="WM704_000457"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFA9339950.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIGSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            27266..28138
                     /locus_tag="WM704_000458"
     CDS             27266..28138
                     /locus_tag="WM704_000458"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HFA9339951.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(28198..28809)
                     /locus_tag="WM704_000459"
     CDS             complement(28198..28809)
                     /locus_tag="WM704_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFA9339952.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCALSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            28917..29141
                     /locus_tag="WM704_000460"
     CDS             28917..29141
                     /locus_tag="WM704_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFA9339953.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            29172..>29548
                     /locus_tag="WM704_000461"
     CDS             29172..>29548
                     /locus_tag="WM704_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339954.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNA"
BASE COUNT         6561 a         7110 c         8821 g         7056 t
ORIGIN      
        1 gacttgtatt ttttccgtgc atcgcgcccg taaggcggaa gcggcgggca atgcgtggcg
       61 gaatgtgggt aaaggcggca ttttgatttg tcggaatgct ggagaacctc tccctttaaa
      121 acgccgtctg aacaaggttg ccggaatagt attgccatcc cggcagatac agtttgtcgg
      181 gatcttgcca atattgttca tccagactgt tcggcagcga ggcggttttg ttgtcgagat
      241 gttttgttaa tccactatat atcccacatt tcttttaggt ttttaccttc cgattggagg
      301 tggcggattt cttttcccaa tgcgtctgcc gcccgatgga tcagcattgc gctgtgtacc
      361 gcgccgattt ttttcagaac ggaacaagtc ttttcggcgg cgggaagacc ccagttgcag
      421 acaaattgca gtatgccgcc gttgccgatg tcggcttccg cccgccaaat caaaaccagc
      481 tcctgttccc gtccgtccat gctttcgagt ttgccgtcat gctgttcaaa aagtttgtcc
      541 accgcctgcc gcatcatctg ctcgaagcgg tcggcttggg tatccgtatc ggtcatagta
      601 ttcttgcctt ttaaaaatgc cgtctgaaca tttcttcaga cggcatttgg gggttaagct
      661 gacatttccc gccagcgttt gacttggagg cgtacctgtt cgggcgcggt gccgcccaag
      721 tggttgcgcg cgttgaggct gccttcgggt gtcagaacgc cgtacacgtc gtctgaaatc
      781 agcttgctga agccttgcag ggcttcgagc ggcaattcgc tcaaatcaac gccggcttca
      841 tcggcgtggc gtacggcttg ggcgacgact tcgtgggcat cgcggaaggg catacctttt
      901 ttgaccagat aatccgccaa gtcggtggcg gtggcaaaac cctgcatcac ggcggcgcgc
      961 atattgccgg gtttgacggt tacgccgcgc atcatatcgg cgtaaatccg cagcgtgtcg
     1021 atgagcgtgt cggcggtgtc aaacagcggt tctttgtctt cctgattgtc tttgttgtat
     1081 gccaagggct gcgatttcat cagggtaatc aggccgataa ggtgtccgat gactcggccg
     1141 gatttaccgc gcacgagttc gggtacgtcg gggtttttct tttgcggcat gatggacgaa
     1201 cccgtacaga agcggtcggc gatgtcgata aagccgaaac gcggactcat ccacaaaatc
     1261 aattcttcag acaggcggct caagtgaacc ataaccagcg aggcggcggc ggtgaactca
     1321 atggcaaaat cgcggtcgga tacggcatcg agcgagttct ggcagatttg ttcgaaaccc
     1381 aacaattcgg cggtgatttc gcgctgaatc ggataggtcg taccggcaag ggcggcagcg
     1441 ccgagcggca tacggttgac gcgtttgcga cagtcggcca tgcgttcgaa atcgcgtccg
     1501 agcatttcga cgtaggcgag caagtggtgt ccgaagctga ccggctgggc gacttgcaga
     1561 tgggtaaaac cgggcatgac ggtttcggcg ttttgttccg ccaaatccaa taatgccgtc
     1621 tgaaggtttt gaatcaggtt ttggataacg gtaatctggt cgcgcagcca aaggcggatg
     1681 tcggtggcga cttggtcgtt gcggctgcgg ccggtgtgca ggcgtttgcc cgcgtcgccg
     1741 attttgtcgg tcaggcggcg ttcgatgttc atgtggacat cttccaaatc gagcggccat
     1801 tcgattttgc cgctgcggat ttcttcgagg atttccgaca tgccccgacg gatgtcggtc
     1861 agatcgtttt cactcagcac acccgaccgg gtcagcatct gcgcgtgggc gagcgagcct
     1921 tggatgtccc attcggcaag gcgttggtcg aaaccgatgg aggcggtgta ttgtttgacg
     1981 agttcggaaa cgggttcgtt gaaacgtccg gaccaggttt tgtcgtgcat aaggattcct
     2041 tgatggggtt attcggtgcg gtatttttcc aaaaggcggc ggaagggttc gccggtttcg
     2101 gggtgtttta aaccgtaggc gacggtggct tcgagatagc cgagtttgct gccgcagtcg
     2161 tagcgcgtgc cttcaaaggg gtgcgccaag acaaattcgt gatcgagcag cttggcgatg
     2221 ccgtctgtaa gctggatttc gttgcccgcg ccgcgcggca ggccggttaa aaggtcgaaa
     2281 atgcgcgggg tgaggatgta gcgtccgaca acggcaaggt tggagggcgc gtcttcgggc
     2341 ttgggttttt cgacaatgcc ggtaatgcgt tggaactgtt tgagctgttc ggtttcgacg
     2401 atgccgtatg agccggtttg cgacggttcg acggtttcta cgcccaaaat gctgttaccg
     2461 ctgcgcccgt acacttcgac catttgtttg agcgcgcctt tgggcgcatc gattaagtcg
     2521 tcggcaagga tgacggcgaa gggttcgtcg ccgatggcgg cgcgggcgca caagacggcg
     2581 tgtcccaatc ccagtgcttc cgcctgacgg atgtagaggc aggtaatgtt cggcggcagg
     2641 atgttgcgga cgtgttccaa caatttgtct ttatggcgca tttccaactt agtttcgagt
     2701 tcgtatgcct tgtcgaaatg gtcttcgatg ctgcgcttgt tgcgcccggt aacaaacacc
     2761 atttccgtgc agccggcttc cacggcttct tctacggcgt attggatcag cggcttgtcg
     2821 acgatgggca gcatttcttt cgggttggcc ttggtggcgg gcaggaagcg ggttcccatc
     2881 cctgcaacgg ggaagacggc tttttttatc ggtttcattc tttttccttt gtattgtttt
     2941 gatgtttaaa gggcgagttt gcggattaaa tcggcgagtg cctgcgcgcg gtggctttcg
     3001 cggtttttga cctccgaatc caattcggcg gcggttttgc cgtgttcggg cagataaaaa
     3061 tacgggtcgt aaccgaaacc gttttgcccg agcggcgtgt cgtgccattg tccgtgccat
     3121 atgccctcgg cgataatcgg gcgcgggtcg tctttatgac ggacaaaaac caatacgcag
     3181 acataagaac aacttttgtc ggccttgccg gcaagttcgg cggcaagttt caggttgttg
     3241 gcggtatcgg atttgggatt gctgccggcg taacgtgcgg aatggatgcc cggcgcgccg
     3301 tttagggcgg cggcacagat gccgctgtcg tcggcgagtg cgggcagccc gctgtgtccg
     3361 gcggcgtgcc gggcttttgc cagcgcgttt tcgacaaagg tgggataggg ttcggggcat
     3421 tcgggtatgc cgaatgggga ttgcggcaat acggtgatgc cgtaaggttt gaataagttg
     3481 ccgaattctt tgagcttgcc ggcattgccg cttgccaaaa cgattttttc cggtttttca
     3541 gacatagcgg tttccctgtg atgaagatgg ggcggcgcgt agggatttgt gccgcaggta
     3601 gagggcgagt gcgccgattt gcccgaacag cgcgccgaat gcgaaaagga aggacgcgac
     3661 ggcgaaggct ttgctgccgg cggtcagcag ataagcaccc aagaggacgg gcagcataaa
     3721 tgccagtgtg aacaaggcaa cagacaacag atagattttt cggcggttca tggcgttcgg
     3781 tcggaaacgg gatgttcgga ttatagccga ttaggacggc attccctaga ggctggaaaa
     3841 atgccgtctg aagcggcgtt cagacggcat ggcgggcatt atgcctgttt gttccaacgt
     3901 tcgatggatt ctttgatgac ttttttcgct tcttccgcac cgccccagga ttcgactttg
     3961 gtcgtacccg cttttttcag gtctttgtag tggttgaagt ggaactcgat ttgtttgatg
     4021 agctgttgcg gcaaatcgga caaagttttg taggcgttgc cgttattgcg gtcgtcggca
     4081 ggtacgcaga cgattttgtc gtccacttcg ccgtcgtcga cgaatttcat cacgccgata
     4141 acgcgcgctt ccaagaatac gccggttgcc agaggttgtt cggtaacgag caacacgtcc
     4201 aattcatcgc cgtcttcgtc caaagtttgg gggatgaagc cgtagttggt cggtttggcg
     4261 aagatggcgg gttcgatgcg gtcgagttgg aatgcggcga gtttgcggtt ccactcgatt
     4321 ttgtggttgc tgccggcggg gatttcgttg acgacgttga tgatgccgcc gtccacgtcg
     4381 cccggggtca ggatttggtt gaagtctgcc atttggtttc ctttatttgg gaaggtttga
     4441 agtttgaaag tatagcacaa acgtccggct ggaaatgcgt ccgatgccgt ctgaaaggga
     4501 aaccgggcgc gtgttaccgt ttgcccaaaa acctgcccag ttccaaaatc gcgcgcctgt
     4561 tggacgggga aaacactttt tccgccgctt cttccaagcc gaaccagccg taggagacgt
     4621 gttcctcggg ttgcaggacg acgggcgtat cgcgcgggat ttcggcccgg aagacgtgtt
     4681 cgcggttttc aaacacgcct ttggggtagc ggtgccgcca gtggtggtag atttcgtaaa
     4741 ccgtgctgtc gtgccggtct tggagctgcc cgtcctccag caggatgccg gtttcttccc
     4801 aaacttcgcg ccttgccgtt tgggcgacgg tttcgcccgg ttcgaggctg ccggttaccg
     4861 actgccaaaa tcctttcgga tgcgtgcgtt cgatgagcag gatgccgccg tccccgctat
     4921 aaaggacgac cagtgcggaa acggggtatt tgagcggttt tgccatcggc atctttcggc
     4981 gggctgcggt aatgaagggg cggattatag caaacgccgc acgttatggc gtttatcctt
     5041 ttccgtatcc ttttttctgc acggatggga cgcgccggtg tttgccggta aattttcctt
     5101 tgtgtcaaaa agataagggc ggttgtgatt ttaatgcttg ccaaagtgac gggcggaaac
     5161 tataatccgc gacttaccga gtcggagtgt ggcgcagtct ggtagcgcac ttgcatgggg
     5221 tgcaaggggt cgaaggttcg aatcctttca ctccgaccaa aaattccgaa agccgctttc
     5281 aaaagcggct tttttgccgt ccgtatgatt atgatgtaga gtacgcggcg acagacattc
     5341 aaatgccgtc cgaaaaccgt tcagacggca tctctttatc ttaatagtgg attaacaaaa
     5401 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc gaggcaacgc
     5461 cgtactggtt taaatttaat ccactatagt ttcattccgt accatcttaa ggaacatcaa
     5521 attgggcatt tcccgcaaaa tatcccttat tctgtccata ctggcagtgt gcctgccgat
     5581 gcatgcacac gcctcagatt tggcaaacga tccctttatc cggcaggttc tcgaccgtca
     5641 gcatttcgaa cccgacggga aataccacct attcggcagc aggggggagc ttgccgagcg
     5701 cagcggccat atcggattgg gaaacataca aagccatcag ttgggcaacc tgatgattca
     5761 acaggcggcc attaaaggaa atatcggcta cattgtccgc ttttccgatc acgggcacga
     5821 agtccattcc cccttcgaca accatgcctc acattccgat tctgacgaag ccggtagtcc
     5881 cgttgacgga ttcagccttt accgcatcca ttgggacgga tacgaacacc atcccgccga
     5941 cggctatgac gggccacagg gcggcggcta tcccgctccc aaaggcgcga gggatatata
     6001 cagctacgac ataaaaggcg ttgcccaaaa tatccgcctc aacctgaccg acaaccgcag
     6061 caccggacaa cggcttgccg accgtttcca caatgccggc gctatgctga cgcaaggagt
     6121 aggcgacgga ttcaaacgcg ccacccgata cagccccgag ctggacagat cgggcaatgc
     6181 cgccgaagcc ttcaacggca ctgcagatat cgtcaaaaac atcatcggcg cggcaggaga
     6241 aattgtcggc gcaggcgatg ccgtgcaggg tataagcgaa ggctcaaaca ttgctgtcat
     6301 gcacggcttg ggtctgcttt ccaccgaaaa caagatggcg cgcatcaacg atttggcaga
     6361 tatggcgcaa ctcaaagact atgccgcagc agccatccgc gattgggcag tccaaaaccc
     6421 caatgccgca caaggcatag aagccgtcag caatatcttt atggcagcca tccccatcaa
     6481 agggattgga gctgtccggg gaaaatacgg cttgggcggc atcacggcac atcctgtcaa
     6541 gcggtcgcag atgggcgcga tcgcattgcc gaaagggaaa tccgccgtca gcgacaattt
     6601 tgccgatgcg gcatacgcca aatacccgtc cccttaccat tcccgaaata tccgttcaaa
     6661 cttggagcag cgttacggca aagaaaacat cacctcctca accgtgccgc cgtcaaacgg
     6721 caaaaatgtc aaactggcag accaacgcca cccgaagaca ggcgtaccgt ttgacggtaa
     6781 agggtttccg aattttgaga agcacgtgaa atatgatacg aagctcgata ttcaagaatt
     6841 atcggggggc ggtataccta aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt
     6901 tgataggaag cttaataaat tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaac
     6961 gagaagaagg agtcagagta gtcagtttaa agcccatgcg caacgagaat gggaaaataa
     7021 aacagggtta gattttaatc attttatagg tggtgatatc aataagaaag gcacagtaac
     7081 aggagggcat agtctaaccc gtggtgatgt acgggtgata caacaaacct cggcacctga
     7141 taaacatggg gtttatcaag cgacagtgga aattaaaaag cctgatggaa gttgggaggt
     7201 gaaaacgaaa aaaggtggga aagtgatgac caagcacacc atgttcccaa aagattggga
     7261 tgaggctaga attagggctg aagttacttc ggcttgggaa agtagaataa tgcttaagga
     7321 taataaatgg cagggtacaa gtaaatcggg tattaaaata gaaggattta ccgaacctaa
     7381 tagaacagca tatcccattt atgaatagta atatttatga aaaattagga gattaatgat
     7441 gaaaagaatt aagtgctttt gtgataaatt tccatcagga gatacattta gaatgtgtat
     7501 cattctggat gactatgata atagggttga ttattatgta ggaatatatg attacattac
     7561 gtctacctta atgagcgata tttactatcg atccacgatt gatgagcatt tcaagattat
     7621 agaattaata gaaaataatc caaatgaaat ttatgatgat ggcggtggtc aacaattttg
     7681 cctagaattt catcatgata aggtcatttt ttaccacaat gaatttgatg aagaagatgg
     7741 ttatccagta ttaagctgtt cgctgcatac ttttaaaact gctttaattg cttggaatgc
     7801 ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag actgtgattg aggaataagc
     7861 ataattagct taatgaatag aatcagcgat atagattgga ctgcaaatcc acgcttatac
     7921 gctgtgccat gattaacagg aagagagcaa cttggatggg ctagtaggga tggtaagcat
     7981 ttaaatatat caattgatgg aaagattaca cacaaatgac taatttaaaa ttagatttct
     8041 actctgaagt tattataaaa gattcttgcc ctaatgattt gttagaaaat ggtgagacta
     8101 ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga agatggtata atctatggct
     8161 atactatttt actttttgat ataaaatatt gtatatatat agataagaaa tatattatac
     8221 cgactggaaa aaaattctct cgcgatgatt tttattagct aatttaagta atttaagcaa
     8281 gttttttaga tactgaaact ggtaattatg tttatgtaag cataaggagt tcagattgaa
     8341 ggattctaca gggcaaatga ggctggcaac caaggatttg gcggaagcca ttaaacgagg
     8401 agaagtacgt agttctgctt ttacaacaaa gcaactaaag gcaatcgaaa aaggagaatc
     8461 taaaatacca gattacactt ggcatcatca tcaagataca ggaaggatgc aattgattcg
     8521 tgaaggcttg catcatgata ccggccatat cggttggaga gcgatgagta aaggaaagta
     8581 actatgtgga aaatcataaa agaggatagt gatgatttag gatttgcaat taaatgctta
     8641 ttctctcaat ctattgattt aaatgaattc aagttatgga ttgaacaagt aatacgcgat
     8701 atgcccatcg aggacatccc tttttatatt tttgacttgg cggatttcaa tgggggaatt
     8761 ggagacattg acaatattgt aggttttgtt tcgagttaca gcctatcaaa atcgaaaaaa
     8821 aatgccttga caggcattgc cttcttaagg gggatagatg tctatagacc ctcccgtttc
     8881 aaaagaaaaa gcattaaaag ccttagagaa atatcctgaa atttatcata aatttaaatg
     8941 gttctttcct tttgtagagc ttccgcttct ttaaaggaca atatgccgtc tgaaaagttt
     9001 tcagacggca tttttatttc ttccagtagg cgggggtgaa gaggatgaag acggtgaaga
     9061 tttccagcct gcccaagagc attgcggtaa cgcagatcca tttctgcatc acgtccaaac
     9121 cggcgtaatt gccggcgggc ccgacttcgc ccagtccggg gccggcgttg gtaatgcagg
     9181 cgatgacggc ggtaaaggcg gtggtaaatt ccataccgct cgccatcagc aggaagctga
     9241 agaggacgac ggtcataaag tagatgaaga tgaaggacat aacggtcagc gcgaggcggt
     9301 cgggtatggc tttgccgctg attttgacgg tgcggacggc tttggggtgc agcagcacca
     9361 tcatttcgcg caggctgaat ttgaacagga cgagggcgcg tatggttttg atgccgccgc
     9421 cggtcgagcc ggagctggcg aggatgttgg cgaggaaaaa catccacagg gaaatcagga
     9481 gcggccattg tgcgaagtcg gtgttggaca gcccgtttgc caatccgatg gagacgaagt
     9541 tgaaggcggt gtagcgcagg gattcgccga agccggcgta atggccggtg tgccacaggt
     9601 acagggcggc ggcaaggatg ctgccggaga gcagcagcag catcgtccgg cattcttcgt
     9661 ctttccaata ggttttgagg ctgcggctgt tgagggcggc gaaatggctg gcaaaattga
     9721 tgccgccgaa aatagtgaaa acgatgatga ctgcttcgat gaggggggag ttgtaataac
     9781 cgatgctggc atcgtgggtg gaaaacccgc ccagcgagag ggttgccagc gcgtgacaga
     9841 cggcatcgaa ccaacccatc ccggcaaaat gcaggcaggc tgccgcgagg atggtgatca
     9901 gggtgtagcc gaaccagagt tttttcgcca cttgggaaat gcgcggcgac attttgcttt
     9961 ctttgtcaat gccggggatt tcggctttga ataactgcgt gccgcctacg ccgagcatag
    10021 gcaggatggc gacggcaagg acgatgatgc ccatcccgcc cagccagttg agcatatgcc
    10081 gccaaaagtt gacggagggg gcgagcccgt cgacgtgggg gatgacggtc gcgccggtgg
    10141 tggtcagtcc cgacatcgat tcaaaaaatg cgtcggtaaa gcccatattc gggaaataca
    10201 ggtacatcgg catcgcagcc atagcggcaa acgccagcca caacatcagg acgagggtga
    10261 agccgtcgcg cgggcgcagt tcgcgcctga accggagggt ggcgagccgg acgatgcacg
    10321 agccggaaag ggtaacggtc gcggtggtgg cgaatgcggt gtacgcgccg tccgaaaagg
    10381 cgtaggaaag ggcggcgggt atcagcagga taaaggaaaa cagcataccc agtcgggaga
    10441 ggacgtgggc gatgggcagg attttgtgca tagtggggcg gtccgttatt ttgcgaagct
    10501 tttccagtct atgccgccgg cggcttggac ttgggtaatt tcttccgttt cgaggttgac
    10561 ggcggtcagc tgcccgcccc acagcgcgcc ggtgtccagc gagatgacgt tgtcggcatt
    10621 cgtgtagccc agcgaggacc agtgtccgaa gatgatggtg tggtcgaggt tttgccgatc
    10681 gggggctttg aaccacgggc gcaggtaagg cggcattttt ttcactgtgg atttgtagtc
    10741 gaaatccagt tcgtttttaa aggtcagggc gcgcatccgc gtgaaggcgt tgacgatgaa
    10801 gcgcaggcgg gcatagcctt ccaaaccttc gtcccatgcg gccggtttgt tgccgtacat
    10861 tttggagaag aatttgacgt attttttgcc gcgcagttcg gcttcggctt ctccggcgag
    10921 cgattcggct ttggctatgc gccattgcgg caggatgccg gcgtgtatca tcacgcggcc
    10981 gccctcgcgt atcaaaagcg gttgcgcgcg cagccagtcg agcatttttc cgccgtcggg
    11041 gtgtttgagt atgggttcga ttgtgtcgct gcgtttgagc gcgccttcgc cgcagccgac
    11101 ggcgagcagg tacaggtcgt ggttgccgag gacgatttgc acgctgtttt cgtgccggat
    11161 gcagaattgc agcgtttcga gggatttcgg gccgcggttg acgatgtcgc ccgtcagcca
    11221 gagggtgtcc gtgccgtggt tgaagccgat tttgccgagc agcgcggtca gttcgtcgaa
    11281 acagccttgg atgtcgccga ttgcgtaatg tgccattgcg ggtgttgtga agtgggaaag
    11341 tgttgcggtt cggacggcac ggttggaaat cttatgccgt ccgaacgtgg aattatgcgt
    11401 tcaaaacgag gacggcttcc gcttcgacct gcacgccttt gggcagcgag gcaacgccga
    11461 cggcggcgcg ggcggggaac ggctcggtga taaattccgc catcacttcg ttgaagacgg
    11521 caaaattgcc caagtcggtc aggtaggcgt tgagtttgac gatgtcgtcc agcgaaccgc
    11581 ctgccgcttc ggcgacggcc tgcaggtttt ggaacacttg gcgcgcttcg gtgtggaaat
    11641 cgccgttgcc gaccacagtc atcgtggcgg gatcgagcgg aatctgaccg ctcatgtaaa
    11701 cggtgccgcc ggcgcgcacg gcctggctgt atgcgccgat ggcggcgggg gctttgtcgg
    11761 tatggatgac ggttttggac atttcggatt cctcaaaaaa tagggcggca gaagccgcag
    11821 cgttcgggat tatcgtacaa aaccgccggc ttgtgtagtt ttaggcggta tatgtgccgg
    11881 aaacaaaaaa ccgccgaagg ctcggcggtt tgcggaataa cgcgtatatc agaatttgac
    11941 gcgcacgccg gcggacagtt cgccggaacg gacgtttttg acagtgttga ctttgccgac
    12001 gtagttgtag cggtagccgg catccagatc gacattgggt gtaacggcat agcttacgcc
    12061 cgccaatacg ccgaggccgg cggaggtttt gctgaagctg tcgctgccgc ccaagtgggc
    12121 ggaagcgcgg ttgaggctca agcgcgcgcc gaaatacggt ttgacgggcg attgggtgtc
    12181 gaagtcgtaa atgacggacg cgccgatgct gtaaagtttg aaatcggtgg atggggcttg
    12241 tttatagttt ttgtagcgcg tgtaatcgac ggcgaagcgg aggtcgttga tgcggtagcc
    12301 tgcggagatg cgcgggctga agcctttggc agaacctaaa gagcttgagg ctttggcgtg
    12361 tgcggcatcg gcttggacgt aaaagccgga tgcgccttcc gccagtgcgg cggccgggag
    12421 tgcgagggca atcagtgcgg caagtgcttt tttcatattt tggttccttt atggtcaggg
    12481 agaaaaattg ttaataatcc gttaaagaat cctgccgtat tatactgaaa ttttcttttt
    12541 gcatcgtaat attttcaatg cctcaagata cgcggcggca tccggctgct ttgccgacgg
    12601 caaagccgtt aacccgcgcg ttgcccttaa acggcggcat cacgcggcgg atgggtgaaa
    12661 cttgcaaacg gtttggaaaa aaccgcaaag gcggggcggc ggcatttgtc agattgttgc
    12721 aggcgcaggc gtacggtttt ttgtgtgcgg cgttacctta ggcgtcggac atttccggcg
    12781 gcggctgtgc cgtctgaaac gcccggcggg ggatgcggct gcgtttccca tcgataggca
    12841 tatttgccgg ccgcgttcgg ggcgggtttt acccgggcgg ccgccgattt gtttgcgctt
    12901 ataaaaaaca ctgcaacaaa tcgtttaaaa acaagcgtcc tttttcggtc gggcggaata
    12961 cggtcgggtc ggtttccagc aggccttttt gccttgccgt ttcgatttgc accatgattt
    13021 tggcggcggg tacgcccgtg cgctcctgca acatcgcggc gggtacgccg tcggtcaggc
    13081 gcagggcgtt catcatgaat tcgaacggca aatcttcggc ggcgacggtt ttgcgttcga
    13141 cggcttcgcc cggctgactt tgcattaagg cgaggtagtc gttggggtgg cggcggcgga
    13201 cggtgcgctc gatgcggtcg ggataggaga ttttgccgtg cgcgcccgcg cctatgccca
    13261 aataatcgcc gaactgccag tagttcaaat tgtggcggca ctgcatggtg ggtttcgcaa
    13321 aagccgatgt ttcgtagtgg ataaaacccg cgccttccag tgtgccgtgt accgcgtctt
    13381 cgatgtcgag cgccgcttcg tcttgcggca aacctttcgg cggcgtatga ccgaacggcg
    13441 tgttcggctc catcgtcaga tgatatgcgc tgatgtgggt tgcgtctgtg gcgatggcgg
    13501 tttgcacgtc gtccaatgcc gtctgaacgg tttggttcgg cagggagtac atcaagtcga
    13561 tattgacttt atcaaataat tttaaggcgg tatcgatagc ggttaaggct tctttgccat
    13621 tgtggacgcg ccccagcctt gagagcatat cgtcgttgaa actctgtacg ccgatagaaa
    13681 gccgcgtgat gcctgcgtct ttaaatcctt gaaacttctc gatttcaaat gtacccggat
    13741 tggcttccaa cgtaatttcc gcttcgggct gcaagcgcaa cagcgaacgc acgccgctta
    13801 acaaacggtc aatcgattcc gcctgaaaca ggctgggcgt accgccgccg aaaaatatcg
    13861 tttccaccgg cctgccccaa atattgggca attcaagctg caagtcggtc agcagcgcgt
    13921 cgatataggc ggcttcgggc aatccgtttt tcaggctgtg ggaattgaag tcgcaatacg
    13981 ggcatttttt gatgcaccac gggatgtgga tatagagcga caggggcggc agggcggtga
    14041 gtcgggtgcg gtttggaaag gaaatggtgt gcatggtgcg gttcggaaaa gtggacgatg
    14101 ccgtctgaag gcggttcgga cggcatgggt tcagcagagc agggtaagca gttcggcttc
    14161 gctgaggacg gaaacgccca aggcattggc tttttccagc ttgctgcccg cggcttctcc
    14221 ggcgacgacg tagtcggttt ttttggacac gctgccggac actttgccgc ctgcggcttc
    14281 gattagggct tgggcttggt cgcgtttgaa ggtgggcagg gtgccggtta acacaaaggt
    14341 tttgcctgcc acggctttat tgatgccgtc tgaaccttgc gccgcctcgt cttcagacgg
    14401 catttgcgcg aagaaggttt ttaggttttc gagcagggcg gtgttttgcg ctttgctgcg
    14461 ccacgcctgc cagtcggcag ggagggcttt gtcgttttgc agtccttcta tactttgtcc
    14521 ggcgagttcc cataaggctt gggctttgtt ttcgctgatt ttgaaaccgg gcaggcgggt
    14581 aatccagcgt tgcggcccgg catattgtgc ggcaggcagg ctgacggctt gggcttgcgg
    14641 ggcaacgcct gcggcgagca gctcgtctat catcgcctgc tgttcggctt gggcgaagaa
    14701 gtgggcaatg gaacgcgcca ctaccgtgcc gatgtcgggc aggcaggcga ggacgggttc
    14761 gggggcgcgg cggacgcgtt ccaatgtgcc gaatgcctgc gccagcgttt tggcggtgcg
    14821 ttcgccgacg tggcggatgc cgagcgcgaa caggaagcgg gcaagttcgg gcgttttgcc
    14881 ggattctatg cctgcgagga tgttttgcgc ccacttggtc ggttgttttt tgccgttttg
    14941 ggtattgtat ttagacaggt cgcctgaaac cgtttctgcg tcgccgtttt cgttttcaga
    15001 tgacccttta tccgccgttt ccttcatttt ttgcagggtc ggaatatcga tgcggtagag
    15061 gtcggcgaaa tggcggacga ggtcttgggc gaccagctgt tcgatttgtt tttcacccaa
    15121 gccgtcgatg tccatcgctt tgcgcgaggc gaagtggatt aagccttgcg cgcgttgtgc
    15181 ctgacaaagc ataccgccgc tgcatcgggc gacggcttcg ccttcttcgc gttcgatttc
    15241 gctgcggcag atggggcagc gggccggcag gcggtagggc ttatggagcg gaacggattc
    15301 ggtttgcttg gcggacggtg tttcggcaaa caggtcgtcc tgctgatgcc cgatgccgtc
    15361 tgaaacggca acggcggttt cctgcatcgg gcggcgttcg aaaatcacgc gcacgacttc
    15421 gggaatcacg tctccggcgc ggcgcacgac gacggtatcg ccgacgcgca cgtctttgcg
    15481 cgatacttcg tcctgattgt gcagggtggc gttggtaacg gttacgccgc cgacgaatac
    15541 gggttgcagg cgggcaaccg gcgttaccgc gcccgtcctg ccgatttgca cgtcaatcgc
    15601 ttcgacaatg gtcagggctt cttcggcagg gaatttgtgg gcaaccgccc aacgcggcgc
    15661 gcgggagatg aagccgagtt cgcgctgttg cgccaagctg ttgactttga ccaccatgcc
    15721 gtcgatttcg tagggcagtt cggggcgttt ttgctgcatg tgttcgtaaa acgccaatac
    15781 ttcgccgata tttttgaaac agccgaaatt gccattgggc agactgaagc cgagttcttg
    15841 gaaataggcg agttcttgga tgtgttcttc agcttcgaaa ccgccttgct gacgggcaat
    15901 cgaatagggg aaaaagtgca gtttgcgttg cgcggtgatg cgcgaatcga gttggcgcag
    15961 gctgccggcg gcggcgttgc gcggattggc aaagggtttt tgcccgtttt cggcttggcg
    16021 tttattgagc ccggcaaagt cggctttgag catcagcact tcgccgcgta cctcgatgag
    16081 ttcgggcacg ttttcgccgt gcagccgcag ggggatgttg gcgacggttt tgacgtttcg
    16141 ggtaacgtct tcgccggtcg taccgtcgcc gcgcgttgcc gcctgcacca atacgccgtc
    16201 gcggtagagc aggctgatgg cgaggccgtc gaatttgggt tcgataacgt attcgggatt
    16261 gccgccgtcc aagccgtcgc gcacgcgttg gtcgaaggcg tacatttcgg catggtcgaa
    16321 cacgccgttt tcatcttgcg gggaaaaggc gttggtcagc gacagcatcg gcacttcgtg
    16381 gcgcacttcg gcaaagcctg ccaaaggctc tccgccgacg cgctgggtcg ggctgtcggg
    16441 cagcttgagc tcgggatggt ttcgttccaa cgcttcgagt tcgcggaaca ggcggtcgta
    16501 ttcggcatcg ggtatgctgg gcgcgtcgag ggtgtagtat tcgtaggcgt agcggttgag
    16561 gaggtcggtg agttcgtgta tgcgttgtgc ggtcgggttc atggattcgt acaggttcag
    16621 acggcatgga caaatgccgt ctgaacggta aaacgtgttg gaaatggtcg gattttacct
    16681 gaaaacaaaa ggcggatgca ccgttgccga tgtatccgcc tttgtgtttg cggagttatg
    16741 aaaacaggcg cagggcggtt ttgccgcccg gttcgatacc gactttgagc atctcggact
    16801 gacgcgccaa tacataagtg cgtacgtctt tgagccattg ggtcgaaact tcttccattt
    16861 tgtcgttgac cagattcagg ttcaactgac cggacaggcg taccgccaaa tccataaaca
    16921 aatcgtcgaa ggttttttcg cctgccggag agtgcgggat gtcgagcagc atactgaagc
    16981 ctttgtagga ctggttgtcc aaaagggcat tggtaaacgg ctcgttgttg agcgagcaga
    17041 tggagaacat ggtcgagccc gacgtgtcgg tatagtggaa cgcgccgtcg tcttccaaaa
    17101 cgaaacccac gcccgttacg gcggaacgca gttctacgcc gctgatgctg gtcggcgaaa
    17161 ccaaatggat ggcgatggtc tggtcgacgc gcgcgcagaa tgcgtccagt gcggaagcca
    17221 cttcgataaa ggcggcaagg tcggtgtgca gcgtctgacc gcccatgctt tgtgcgaatg
    17281 cgtccgcctg gcggttgaat gcggagagtt cttcctgcga ggcaagtccg ttgcggctga
    17341 ctgcctggat acccacgata aatgcctgat agcggatgcc cgggatgggt tcggcaatct
    17401 ggaaatggtc gtccatggtg cagccgacaa tctggtagcg gcagcggttg gaaaggcgcg
    17461 gcagtgcgtg cagttctttg gcttcggtca gcgcgatata ggagatgaag tcgaagcgca
    17521 cgtcaaacca gggcagctcg accttcgaca gctctttgag cgtaatcagc ggttttgccg
    17581 gcgtttgcgg aacgggaacg ggttttgccg gcgcgtcggc aggtttcggt gcggaatgtc
    17641 cggtttgggg ttcggaaacg gtgtgggcgg agttgccgat aatgccgatt tcttccaagg
    17701 cggtttcgat ttcggttttg aacggggagg cttttgcctg tttctgcttg gcgatgtaga
    17761 cggcatcctg ttcttgcagg ttgcgcatgg cggagtcttg gggtttggcc ggttttttga
    17821 ccgccggttg gggtttcggc atcatgactg gcccgccgga cggtttgccg tcgcggacat
    17881 ggctggtttt gctgttgagc agggcatctt tgtcggagtg tccgaactgg tcgcgcactt
    17941 ttttgcggta ttggttttcc tgatacatat tgtaggcgac aacggcgagg acggcggcga
    18001 ggaacagtac gatgtaaatc atggcaatca ctttaaattt cgggatgcag gatgcgcaaa
    18061 gtgcgggtac tgcggttaaa tcgggcttgc gtttccggca gtctgacgga acggccggtt
    18121 ataacgtttg aattataacg aaaattgcag ggtctgacag cagtgtgtcg aaataagcgg
    18181 aaattttccg aaatgccgtc tgaaatctgt ggttttcaga cggcatttct gtccacggga
    18241 aacccttttt cccgtatccg ccgccagtcg aaaaaatggc cggggtcggt tttgcggccg
    18301 ggtgcgatgt cttggtgtcc cgttaccgcc gtgatggggt agcggcggca gagggctgcc
    18361 aacagtgttt cgagcgaacg gtattgcgct tcggcaaacg gttcgaaatc gcagccttcc
    18421 agttcgatgc cgatggaaaa cgcgttgcac ttctcccgcc cccgaaacga ggatgcgccg
    18481 gcgtggtacg ccatgtcgcc gcatgatacg aactgcaccg tttcgccgtc gcgtgcaatc
    18541 aggaaatggc tggacacgtg caaagtgtgt atcaggctga agaacggatg tccgtcgggg
    18601 tcgagccggt tggcaaacag cttttccacc gcatccgtgc cgtattcgaa cggcggcagc
    18661 gaaatgttgt gcagcacgat cagggaaacc gtttctcccg tttccctcgg gctgaaattg
    18721 ggcgacgggg tatggcgtat gctttgaagc cagccgtttt gccagtgtgc tccggcgtga
    18781 ttgtccatga tgttcttcct gtccggcggg caatttgggt tatactgtcg cccgaatttt
    18841 aagacgtatt ccgaatgctg ggaatcctac catgttgaaa aaaatgttga aatggactgc
    18901 cgtttttttg accgtatcgg cagccgtttt cgccgcgctg ctttttgtcc ctaaagacaa
    18961 cggcagggca taccgaatca agattgccaa aaatcagggt atttcgtcgg tcggcaggaa
    19021 acttgccgaa gaccgcatcg tgttcagcag gcatgttttg acggcggcgg cctacgtttt
    19081 gggtgtgcac aacaggctgc atacggggac gtacagattg ccttcggaag tgtctgcttg
    19141 ggatatcttg cagaaaatgc gcggcggcag gccggattcc gttaccgtgc agattatcga
    19201 aggttcgcgt ttttcgcata tgaggaaagt catcgacgca acgcccgaca tcggacacga
    19261 caccaaaggc tggagcaatg aaaaactgat ggcggaagtt gcgcccgatg ccttcagcgg
    19321 caatcctgaa gggcagtttt ttcccgacag ctacgaaatc gatgcgggcg gcagcgattt
    19381 gcagatttac caaaccgcct acaaggcgat gcaacgccgc ctgaacgagg catgggcagg
    19441 caggcaggac gggctgcctt ataaaaaccc ttatgaaatg ctgattatgg cgagcctgat
    19501 cgaaaaggaa acggggcatg aggccgaccg cgaccatgtc gcttccgtct tcgtcaaccg
    19561 cctgaaaatc ggtatgcgcc tgcaaaccga cccgtccgtg atttacggca tgggtgcggc
    19621 atacaagggc aaaatccgta aagccgacct gcgccgcgac acgccgtaca acacctatac
    19681 gcgcggcggc ctgccgccaa ccccgattgc gctgcccggc aaggcggcac tcgatgccgc
    19741 cgcccacccg tccggcgaaa aatacctgta tttcgtgtcc aaaatggacg gcacgggctt
    19801 gagccagttc agccatgatt tgaccgaaca caacgccgcc gtccgcaaat atattttgaa
    19861 aaaataaacc atgccgtctg aaaagtttgt gttttcagac ggcataccct taccggaact
    19921 gcaagcatga aaccgcaatt catcactttg gacggcatag acggtgccgg aaaatccacc
    19981 aaccttgccg tcatcaaggc atggtttgaa cggagggggc tgcccgtgct gttcacgcgc
    20041 gagccgggcg gaacgccggt cggtgaggca ttgcgcgaaa tcctgctcaa ccctgaaacc
    20101 aaagccggtt tgcgtgcgga aacactgatg atgttcgccg cgcgtatgca gcacatcgag
    20161 gaagtcatcc tgcccgcgct ttcagacggc atccatgtcg tgtccgaccg ttttaccgat
    20221 gcgaccttcg cctatcaggg cggcgggcgg gggatgccgt ctgaagacat tgaaattttg
    20281 gaacattggg tgcagggcgg tttgcgcccc gatttgaccc tgctgttgga tgtgccgctg
    20341 gaagtatcga tggcgcgtat cggacaggcg cgcgagaaag accggttcga gcaggagcag
    20401 gcggatttct ttatgcgtgt gcgcggcgtt tatctcgacc gagccgccgc ctgtcccgaa
    20461 cggtacgccg ttatcgacag taaccgcagc ttggatgaag tcagaaacag catagaaaaa
    20521 gtgttggacg gacatttcgg ctgttgatgc ggcaaatatt gaaacaagcg catccgcccg
    20581 cgccgaaaac caaacggcag tgccgcaggt gaaaatggcg gtatgcgcca aactttcggc
    20641 atgatagaat tacgctcggt tacaaggcag gatgcgtcgg caatattaac gaaccgcccg
    20701 taacatgatg acccgaaagc gtttcggaca gttcgattca aatctttttc tcgcaacagg
    20761 attgacacat ggaaaactca ttgaaagaag ccgccctcaa gttccacgaa ttacccgttc
    20821 cgggcaaaat ttccgttacc ccgaccaaat ctctggcgac cgacaaagat ttggcgttgg
    20881 cgtactctcc gggcgtagcc gccccctgta tggaaatcca tgccgatccg caaaatgcct
    20941 acaaatacac cgccaaaggc aacttggtcg ccgtcatttc caacggtacg gccgttttgg
    21001 gcttgggcga catcggcgcg ctggcgggca aacccgtgat ggaaggcaaa ggcgtattgt
    21061 tcaaaaaatt cgccggtgtg gacgtgttcg acatcgaaat cgacgaaaaa gacccgcaaa
    21121 aactggtgga catcatcgcc gctttggagc cgactttcgg cggcatcaac ctcgaagaca
    21181 tcaaagcacc cgagtgtttc tacatcgaac gcgaattacg caaacgctgc aaaatccccg
    21241 tatttcatga tgaccagcac ggcacggcca tcattaccgc cgccgccgta ttgaacgccc
    21301 tgcgttatac cggccgtaaa atcgaagaag cgactttggt gtgctccggc gcaggtgccg
    21361 ccgcgattgc ctgcctgaac caactgctgg atttgggctt gaaacgtgaa aacgtaaccg
    21421 tttgcgactc caaaggcgtg atttaccaaa cccgcgaaga caaagaccgc atggacgaat
    21481 ccaaaaaatt ctacgccgtt gaagacaacg gccggcgcgt acttgccgat gcggttaaag
    21541 gcaaagacat cttcttgggc ctctccggcg caaacctgct gacgcctgaa atgttgaaca
    21601 cgatgaacga aaaacccatc gtgttcgcca tggccaaccc gaatccggaa atcctgccgc
    21661 cgctggcgaa agaaacccgt ccggacgtgg ttatcggtac cggccgctcc gacttcccga
    21721 accaagtgaa caacgtattg tgcttcccgt tcatcttccg gggcgcgttg gatgtcggcg
    21781 caaccaccat caacgaagaa atgaaacgcg cctgcgtgta tgctttggca gatttggcga
    21841 tggaagaagt aaccgaagaa gtggttgccg cttacggtaa gaaattcgaa ttcggcgcgg
    21901 aatacctgat tcctactccg ttcgattccc gcctgctgcc tcgcgtcgct acggctgccg
    21961 ccaaagcagc gatggaaagc ggcgtggcaa cccgtccgat tgcagatttg gaagcttacg
    22021 ctgccaagtt gggcgaatgg aagctgtaag ccgtttgtgg ttaaaatgcc gtctgaactg
    22081 ttttcaggcg gcattttgtt gtcagattga taaatgaaag atactggaaa atgaaagaga
    22141 tgaaacctgt ccgttatcat attggcgata tacccgaaac ttcaaaacaa accgcccccg
    22201 gtcatgacga cagggcagtg ggtgttgacg atgattgttt tcatgattcc tttggtcaat
    22261 atcgttatgt tttttgtttg ggcgttcggc agaggcaacc ccaaccgtgc caatttctgt
    22321 aaagcactgt tcttatttac cttgttggtt cgcttatcgg tttgattttc atattgctta
    22381 taggtgggtc tgcatcgggt acgcattatt aatgtgccgg ctgattctgc ttcgaagatt
    22441 tgtatcgaat atgccaaatt tttttaaatt tcataccgtt atcgaacggc attggcaaaa
    22501 accttatccg gttttgtctt ttctgctcaa gccgctttcc gggctgtttg ccaaaattgc
    22561 ggcaaaatgg cgggcggatt ttttatcggg aaaacggcaa agcgaaaagc tgtccgtgcc
    22621 tgtggtcgtg gtcggcaaca tccacgcggg cgggacgggc aaaacgccga ttgccgccgc
    22681 gctggtgtcg ggcttgcagg aaaaaggtgt caaggtcggc atcatcagcc gaggctacgg
    22741 gcgcaagggc aaggcggttt atgtattgaa tgccgcgagc cgtgcggaag atgcgggcga
    22801 cgagcctttg ctgctgttcc gtaaaacagg tgcgccgacg gcagtgggca gcagccgtgt
    22861 ggaggcaggc agggcgttgc tggcagcgca tccggagctt gaattgattg tggcggacga
    22921 cggtttgcag cattacgccc tgcaacgcga tgtggaaatc gcggtgtttc cggcggcgga
    22981 tacggggcgt acggatttgg acttgctgcc caacggcaat ttgcgcgaac ctttgtcgag
    23041 attggaaagc gtggatgcgg tggtggtcgg cggcagggcg gcggatggtt ttatgccgtc
    23101 tgaacatttg ttcggcagcc gtattgaggc gggtgcggtt taccgtttga accgtccgtc
    23161 tgaaaaactg gatatttcga cattgtcagg gaagcgcgtc gcagcggttg ccggtatcgc
    23221 caggccgcag cgtttcttcg atactctgac acacatgggc atccgccttg accaaacggt
    23281 tgctctgccc gaccatgccg atattttcaa tcgggatttg ccgcctgccg atgtggtgct
    23341 ggtaactgaa aaagatgcgg tcaaattttc agacggcatt tgcaccgata atgtttgggt
    23401 gctgccggtt tgtgcgataa tcgaacctga tttggcggag tttgtgctgg agcggttgga
    23461 gggtgtaccg aaggccgtct gaaagcgcgg tttggacgaa gtgataacgg actggaataa
    23521 gaacgcccta cgccgtcatt cccgcgcagg cgggaatcta agtctcgaat tttcaggaat
    23581 gcctaggaga cttcagaaat cccaaatctc cggatttcca cttgggcagg aatgagaaag
    23641 ccggttgtat tttttatctg cattaatcat tcgttaaagg agtggatatg aagctgaaaa
    23701 ccttgttatt gcccttcgcc gcactggcat tgtgtgccaa cgcatttgcc gccccgcccg
    23761 gcgacgcgtc gttggcacgt tggctggata cgcagaattt cgaccgggat atagaaaaaa
    23821 atatgattga aggctttaat gccggattta aaccgtatgc ggacaaagcc cttgccgaaa
    23881 tgccggaagc gaaaaaagat caggcggcag aagcctttaa tcgttatcgt gagaatgttt
    23941 tgaaagattt gattacgccc gaagtgaaac aggctgtccg caatacctta ttgaagaatg
    24001 cccgtgaaat atacacgcaa gaagaaattg acggcatgat tgccttttac ggttcgcctg
    24061 tcggtcagtc cgtcgttgcc aaaaatccgc gcttaatcaa gaaatcgatg agtgaaatag
    24121 cggtatcttg gactgcattg tcagggaaaa tcgcgcgaca tcatctgccc gagtttacgg
    24181 aagagttacg gcgcatcatc tgcggcggta tagtggatta aatttaaatc aggacaaggc
    24241 gacgaagccg cagaccgtac aaatagtacg gcaaggcgag gcaacgccgt accggtttaa
    24301 atttaatcca ctataaaaat cccgatgcgg gctgcaagca ggccggacag cttgggaaaa
    24361 ggtatcagaa gtaaataata gccgcctgaa atattgcgga gggcgtccga ttgattaaac
    24421 catcaaaccc gaaagcaacc ttatggaaaa aaaattctta gacatcctcg tctgccccgt
    24481 taccaaaggc aggctggaat atcatcagga caaacaggaa ttgtggagcc gtcaggcgaa
    24541 gcttgcctat ccgattaaag acggcattcc ctatatgctg gaaaacgaag cacgagcgtt
    24601 gagcgaagag gaactcaaag catgaccgaa ttcgtcgtat tgattccggc gcggctggat
    24661 tcgtcgcgcc tgcccggaaa agccttggcg gacattcacg gcaaaccgat ggtcgtgcgc
    24721 gttgccgaac aggcggcaaa aagcaaagcc gcgcgcgtcg tcgtcgccac cgatcatccc
    24781 gacattcaga cggcctgtca ggcgcacggt atcgaagtcg tcatgacttc aaaccggcac
    24841 gaaagcggca cgacgcgcct tgccgaagcc gccgccgcgc tgaagctgcc gccgcatttg
    24901 attgttgtga acgtacaggg cgacgagccg ctgattgccc ccgaactcat cgaccgcacc
    24961 gccgaagtac tcgtcgaaaa caacgtccaa atggcgaccg ccggccacga attgcacgat
    25021 ttcgacgaat tgatgaatcc caatgccgtc aaagtcgtcc tcgacaaaaa cggcaacgcc
    25081 atctacttca gccgtgcccc gattccctat ccgcgcgatg cgatgcgtgc cggaaaacgc
    25141 gaaatgccgt ctgaaaccgc cgtcctacgc catatcggca tttacgccta ccgcgtcggc
    25201 ttcctgcaac gctatgccga aatgagcgtc tcgccgctgg aaaccatcga atcgctggaa
    25261 caactgcgcg tcctgtggca cggctacccc atcgccgtcg aaaccgccaa agaagccccc
    25321 gccgccggtg tggatacgca ggaagatttg gacagggttc gcgctgtatt tcagaccgta
    25381 taaaacaggt tcaaagggaa aagatatgca gcaacatatt gaaaagtggc aacacttgag
    25441 ccgtgaagaa cagaaaatcc ttgccgaagt atggggtctc gtgcaaaacg atgatcagga
    25501 ggttcactat gaaatgctca aattgaacgc acctgatgaa gtcagcggtg aattttggtt
    25561 cagaatggca gaaacactca gcaccctgcc gcccaatcgt tccctcgacc ttagaatgaa
    25621 cggcggcagg ctgtcgaccg ccgtatccat cctttccgtc atgattgaag acaatcccga
    25681 cataccgcag ctttgggcgt aaaaaattac cgccctcaat tatttggtac acggacacaa
    25741 agcccgtgcc gacggtttgg tacaacagcc cgacaaagcg gcagaagcca acgaggagga
    25801 atacctgacc aaagccctgt cgcaaaacct gctgtcaaca ttggatgccg cacttgcacg
    25861 ttttcctgaa gacgcgtggt ttcaggaaat caaacaggat gcacaaaagc attttgcttg
    25921 aggacgtggc ggtcaggaat atttccattc aggaagaaaa gaagtgccta attgggtata
    25981 atcagggtaa atcttatttt atttcagaag attaatattt gttttccgtt tttccttgac
    26041 ggtatcggaa aagttgatta tagttacagc ttccttagga gtaatggctg agaggctgaa
    26101 ggcacttccc tgctaaggaa gcatgtggga tcaacctgca tcgagggttc gaatccctct
    26161 tactccgcca gataaaaaaa tagacgctgt gttttacagc gtctattttt ttatgcaatt
    26221 ttatagcggg ttggcgcaaa cccggtatgg tattgccctg tcttgattct gaattttgtt
    26281 aatccgagat gtttgccgtt tatttttgcc tcgttcaaac ggcggctctg atctgcgcgg
    26341 tttctgtttg ccgtattcgc ctatccgtac cgcaaatgtt atactgggaa aaattcaccg
    26401 attgtatttt acggcgtatt tgccgatagg atggaagaga caaatgagca gaatccggca
    26461 ggcttttgcc gctttggatg gcggaaaggc attgattccc tatattgcgg tgggcgaccc
    26521 cgatattcgg acaactttgg cattgatgca cggcatggtt gcaagcggtg cggatatttt
    26581 ggagttgggc gtgccgtttt ccgatccgat ggcggacggg ccggttattc agcgtgcggc
    26641 ggagcgggcg ttggcaaacg ggatttcgct gcgcgatgtc ttggatgtcg tcagaaaatt
    26701 ccgtgaaacc gacacgcaaa cgccggttgt tttgatggga tatttgaatc ccatacataa
    26761 gatgggttat cgggagtttg ctcaggaagc cgcaaaggcg ggggtggacg gcgtgttgac
    26821 ggtggattcc cccatcgaaa ccatcggttc tttatatcgc gagctgaagg ataacgaggt
    26881 cgactgtatt ttcctgattg cgccgacgac gacggaagac cgtattaaaa ccattgccga
    26941 gctggcaggc ggatttgtct attatgtttc gctcaagggc gtaacgggcg cggcaagttt
    27001 ggatacggat gaggtttcgc gtaaaataga gtatttgcgt cagtatatcg atattcccat
    27061 cggtgtcggt ttcggcatca gcaatgcgga aagcgcgcgc aaaatcggcc gggttgccgc
    27121 cgcgatcatt gtcggcagcc ggattgtgaa agagattgaa aacaatgcgg gcaacgaggc
    27181 tgccgccgtc ggtgctttgg tcaaagagtt aaaggatgcc gtgcgctgat tctgtcgcgc
    27241 attctaaatg ttttaggagt tgtccatgag ctggttagat aaaatcctgc cacccaaaat
    27301 caagaatcgt gggaaagacg gttcttccaa tgttcccgag ggtttgtggc gcaaatgccc
    27361 gtcttgttcg gcaaccgttt attcgaccga gttgcagcag aacaatcagg tctgcccgaa
    27421 atgcaaccac cacaatccgt tatcggcgcg ccagcgtttg aacctgcttt tggatgagga
    27481 aggcagggag gaagttgcgg gtaatgtcaa accgaccgat cctttgaagt ttaaagacag
    27541 caaaaaatat ccggaccgtt tgagtgcggc acgcaagctg accggggaag atgacgcgct
    27601 ggtggtgatg aaggggatga tgaacggtct gcccgtcgtc gttgccgcgt ttgaattccg
    27661 ctttatcggc ggttcgatgg gttcggttgt gggcgagcgt ttcgtacagg gtgtccgccg
    27721 tgcggttgcc gataattgtc cgtttgtctg tgtggcggct tccggcggcg cgcgtatgca
    27781 ggagggtgta aactcgctga tgcagatgac gaaaaccagt gccgcgctgc atttgctgac
    27841 ggaaaaacgc ctgccgttta tatcggtgtt gaccgatccg actatgggcg gcgtatctgc
    27901 cagcttcgca tttttaggcg atgtcgtgct tgccgaaccg aacgcgctga tcggttttgc
    27961 cggtccgcgc gtgattgagc agacggtgcg cgaaacgctg ccggaaggct tccaacgcgc
    28021 cgagtttctg ctggaaaaag gcgcgatcga ccagattgtc gaccgccgcg atatgaagcg
    28081 gcgcatcagt gatttgatta cgctgttgtg ccgtcaggac aaagtttccg ccacctgatg
    28141 gctgatgaat cgaatgccgt ctgaaaccga tgtttcagac ggcatttttg tgtctggtta
    28201 tttgtcgtgc ggcttcatcg acggggcata gcgtccggca cgttctttca ggcgttgtac
    28261 caaaccttgc gtgtcggcgg gtacgccgcc ctcgcagaat gcctgataca ggacggcgcg
    28321 caatgcgtcg ttgcgcccaa gtgtgccgcc tatcggtttc cattcggcgt ttcggggctg
    28381 tatccagcgg ctgttgaccg tatcgccgta tccgaatact ttgtaggagg aaagtttgcc
    28441 gttgccgaac ccgatggagg agagggcgca gaatatgcct tcggcagtca ggttgtcgta
    28501 gcctttgtcg gagcggatat tgagaatgta gcggatgctg ccgtcgggcg cgggcataat
    28561 ttgcaggctg tcgagcagga ttttcggctg tttgccgtaa ttttcatcca cataaatgtc
    28621 aaaccagccg tccgagtgcg catcgggcag aggcggcagt tcggcggtat gttctttaaa
    28681 ttcgcgggcg gcggcttctt cgggcgtttc gcggtagcgg gtgttgatcg gcgtgtcttt
    28741 ttggctgaag ccggcggcaa gggacgtgcc gacagtcagg gtcagaatca gaaggatggc
    28801 gcggcgcata agtttctcca aattgaaaac ggctttattt tatggtttgg cgggaagggc
    28861 tgcaagcaat cggggtataa tctgacctga ttttcatttt aaagcggtgt cgaaccatga
    28921 acagcgaaac tttagacgta accggattga aatgtcccct gccgattttg cgggcaaaaa
    28981 aggctttggc gcaaatgcgg cagggcgagg tattgaccgt tctggcgacc gacggcggcg
    29041 cgccggggga ctttgaggct ttttgccgcc aaaccggtca tgtgctgttg gattcttccg
    29101 aacaggacgg cgtgttcaca ctggtcgtca agcacaaata aatgccgtct gaaatgcgga
    29161 tgtcctgatt gatgttattg ctgtcggcat ttgtgcttgc tgcgtgtaat gcccaaaacc
    29221 ggcaatccgg tgtacggcat caggattttg cacgcgtagc cgtcgatttg tttcggagtg
    29281 cctgcgtcct gactcaaggt agtttcgaac cggtttccaa gtttgccgct gtcggacatt
    29341 ttgtccctgt cggaaaggaa gacctgtccc gtctgccgcc cgctgttgca gaacccgatg
    29401 tacaggcgtt gtggacgctt gaacgcgatg gcggcattta ttatttgagc ctgacgcgcg
    29461 acagttgcag tgtgaaagcc gagcgggcag acagtgccgc gcttttggaa catttctcag
    29521 ggcttgtccg acagccgccg aaaaatgc
//
LOCUS       DAWTHD010000013        28870 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWTHD010000013 DAWTHD010000000
VERSION     DAWTHD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28870
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.013"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            168..2429
                     /locus_tag="WM704_000462"
     CDS             168..2429
                     /locus_tag="WM704_000462"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HFA9339955.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASNRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            2432..3508
                     /gene="nuoH"
                     /locus_tag="WM704_000463"
     CDS             2432..3508
                     /gene="nuoH"
                     /locus_tag="WM704_000463"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFA9339956.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            3589..4068
                     /gene="nuoI"
                     /locus_tag="WM704_000464"
     CDS             3589..4068
                     /gene="nuoI"
                     /locus_tag="WM704_000464"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFA9339957.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            4080..4751
                     /locus_tag="WM704_000465"
     CDS             4080..4751
                     /locus_tag="WM704_000465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFA9339958.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            4748..5053
                     /gene="nuoK"
                     /locus_tag="WM704_000466"
     CDS             4748..5053
                     /gene="nuoK"
                     /locus_tag="WM704_000466"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFA9339959.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            5056..7080
                     /gene="nuoL"
                     /locus_tag="WM704_000467"
     CDS             5056..7080
                     /gene="nuoL"
                     /locus_tag="WM704_000467"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFA9339960.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRTKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGVAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            7223..8221
                     /locus_tag="WM704_000468"
     CDS             7223..8221
                     /locus_tag="WM704_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9339961.1"
                     /translation="MDKRLFDGTVRFEHVTGVGTMQVDFQPNQRVYTMIGENGVGKTK
                     FLECLFTVLLFTYRMENINIGFSWIDLQRLPLKIVELPNGKNEDFRRFSERSNQGFNI
                     NEDVLFSGYQHRLPFVYLPAQSRGWVKKTNFQTQSLGSSQGRASKYLGNLFSVFQSGH
                     SSFSLKSLNMENDLEEWIIQRARSANPYQANEDNRKIEIDTLLFLLNQFDGRIAPDFL
                     EIKGDDRIFIKLDNQKRELSGLSSGFTSILKIMQSIVAGYSYFTNEQQIANVRGVVLI
                     DEIESHLHNEWQIKIVPLLKKLFPNTTFFITTHSSLVISQLEQGGPIVWYVARRTV"
     gene            8233..8391
                     /locus_tag="WM704_000469"
     CDS             8233..8391
                     /locus_tag="WM704_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C nitrite reductase"
                     /protein_id="HFA9339962.1"
                     /translation="MIDYPSNASFVDLLNEAFGVDLNKKKIERAQERNQEQAKKPYWN
                     WYVRNWAD"
     gene            8402..8626
                     /locus_tag="WM704_000470"
     CDS             8402..8626
                     /locus_tag="WM704_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFA9339963.1"
                     /translation="MTVYLLDTNYLVYLADDDSDEEKRKAVLSDMAEKLQQDDNRFVI
                     TPLIRYEVLRGVDWGKSEKLSRLTGVLAQF"
     gene            8657..8863
                     /locus_tag="WM704_000471"
     CDS             8657..8863
                     /locus_tag="WM704_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339964.1"
                     /translation="MASNLYRFDKFEAERDNTPKNLEKRKFDMFHYATASVNNLEILS
                     HDTDVNKIKDLHERMRLEDSAELA"
     gene            8973..10469
                     /locus_tag="WM704_000472"
     CDS             8973..10469
                     /locus_tag="WM704_000472"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFA9339965.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            10479..11924
                     /gene="nuoN"
                     /locus_tag="WM704_000473"
     CDS             10479..11924
                     /gene="nuoN"
                     /locus_tag="WM704_000473"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFA9339966.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            12036..12335
                     /locus_tag="WM704_000474"
     CDS             12036..12335
                     /locus_tag="WM704_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFA9339967.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(12452..13348)
                     /locus_tag="WM704_000475"
     CDS             complement(12452..13348)
                     /locus_tag="WM704_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFA9339968.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(13332..13556)
                     /locus_tag="WM704_000476"
     CDS             complement(13332..13556)
                     /locus_tag="WM704_000476"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFA9339969.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(13536..13643)
                     /locus_tag="WM704_000477"
                     /pseudo
     CDS             complement(13536..13643)
                     /locus_tag="WM704_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(13669..14592)
                     /gene="rsgA"
                     /locus_tag="WM704_000478"
     CDS             complement(13669..14592)
                     /gene="rsgA"
                     /locus_tag="WM704_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFA9339970.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(14597..16453)
                     /locus_tag="WM704_000479"
     CDS             complement(14597..16453)
                     /locus_tag="WM704_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9339971.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMAFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(16479..16616)
                     /locus_tag="WM704_000480"
     CDS             complement(16479..16616)
                     /locus_tag="WM704_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339972.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            complement(16616..17200)
                     /gene="ruvA"
                     /locus_tag="WM704_000481"
     CDS             complement(16616..17200)
                     /gene="ruvA"
                     /locus_tag="WM704_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFA9339973.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(17282..17608)
                     /locus_tag="WM704_000482"
     CDS             complement(17282..17608)
                     /locus_tag="WM704_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9339974.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(17669..18355)
                     /locus_tag="WM704_000483"
     CDS             complement(17669..18355)
                     /locus_tag="WM704_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFA9339975.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKPEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(18483..18947)
                     /gene="trmL"
                     /locus_tag="WM704_000484"
     CDS             complement(18483..18947)
                     /gene="trmL"
                     /locus_tag="WM704_000484"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFA9339976.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(19012..19620)
                     /locus_tag="WM704_000485"
     CDS             complement(19012..19620)
                     /locus_tag="WM704_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFA9339977.1"
                     /translation="MASFRTDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRALKHLADLGMAQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGF
                     NQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            19604..20485
                     /gene="bioH"
                     /locus_tag="WM704_000486"
     CDS             19604..20485
                     /gene="bioH"
                     /locus_tag="WM704_000486"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFA9339978.1"
                     /translation="MRKLAKSVLQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKF
                     MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     GIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLARCGTPQALQEALDAA
                     ERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSH
                     AEVFAALYRDFVEGGLT"
     gene            20482..21270
                     /locus_tag="WM704_000487"
     CDS             20482..21270
                     /locus_tag="WM704_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFA9339979.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPLAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(21351..21458)
                     /locus_tag="WM704_000488"
                     /pseudo
     CDS             complement(21351..21458)
                     /locus_tag="WM704_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            21652..22953
                     /locus_tag="WM704_000489"
     CDS             21652..22953
                     /locus_tag="WM704_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFA9339980.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLCGEDTQRYASFWGDG
                     YDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLENHGQTVLDFSNTRITDLSVDMTGVE
                     SLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLILEVGKSVPKVRGLENLTAVNVMGIA
                     DFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSGFEDLEVFTAVDLFGFGADDIPHPD
                     RLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLDLWIEKARKPEWLAQNFDNPFRDWD
                     GAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPENTGEGLAEAVKAYTGGFNKMDKKHF
                     IDTVEREDIAEALETILDLIPDGSCADKEKLFEIFDKNRNF"
     gene            complement(22990..25293)
                     /gene="recQ"
                     /locus_tag="WM704_000490"
     CDS             complement(22990..25293)
                     /gene="recQ"
                     /locus_tag="WM704_000490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFA9339981.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKILKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(25356..26138)
                     /gene="trpC"
                     /locus_tag="WM704_000491"
     CDS             complement(25356..26138)
                     /gene="trpC"
                     /locus_tag="WM704_000491"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFA9339982.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(26189..27148)
                     /locus_tag="WM704_000492"
     CDS             complement(26189..27148)
                     /locus_tag="WM704_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFA9339983.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRRELLPDSTGKT
                     KTAYDFSAGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGV
                     ANTAPVFYHLGDTAEIAFALDTLAARYREIYAVGVSLGGNAPAKYLGEQGKKALPHAS
                     AAVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFD
                     DRFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEA
                     VTLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(27281..28819)
                     /gene="murJ"
                     /locus_tag="WM704_000493"
     CDS             complement(27281..28819)
                     /gene="murJ"
                     /locus_tag="WM704_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFA9339984.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
BASE COUNT         6079 a         7457 c         8443 g         6891 t
ORIGIN      
        1 aaattcaaaa aatggattcc cgcctgtgcg ggaatgacgg caaggtggca gtaaacgttt
       61 taaacagtat tgattgtcaa tgaaactcaa aaggccgtct gaagcccatt tttcagacga
      121 tctccataaa aaattattta tcaaataccc gtaactagga acgaaccatg ttacaaatcg
      181 aaatcgacgg caaacaggta tctgtggagc agggcgcgac ggtaattgaa gccgcgcaca
      241 agctcggtac ttatattccg catttctgtt accacaaaaa gctctctatt gccgccaact
      301 gccgtatgtg tctggtggat gtggaaaaag cccccaaacc gctgcctgcc tgtgccacgc
      361 cggtaacgga cggcatgatt gtgcgtacgc attcggcaaa agcccgagag gcgcaagaag
      421 gcgtgatgga gttcctgctc atcaaccatc cgcttgattg tccgacctgc gaccagggcg
      481 gcgaatgcca gttgcaggat ttggcggtgg gctacggcaa aaccaccagc cgttataccg
      541 aagaaaaacg ttccgtcgtc ggcaaggaca tgggtccttt ggtttccgcc gaggaaatga
      601 gccgctgtat ccactgtacc cgctgcgtac gtttcaccga agaaatcgcc ggtttgcagg
      661 aaattgcgat ggtgaatcgt ggtgaacact ccgaaatcat gccctttatc ggcaaagcgg
      721 tggaaaccga gctgtcgggc aacgtcatcg atttgtgtcc cgtcggcgca ttgaccagca
      781 aaccgttccg cttcaacgcg cgtacttggg aattgaaccg ccgcaaatcc gtttccgcac
      841 acgacgcttt gggcagcaac ctgattgtac aaaccaaaga ccataccgtc cgccgcgtgt
      901 tgccgttgga aaacgaagcc atcaacgaat gctggctgtc cgaccgcgac cgttttgcct
      961 acgaaggcct gtatcacgaa agccgtctga aaaatccgaa aatcaaacag ggtggcgagt
     1021 ggatggacgt ggattggaaa accgcgttgg aatatgtccg cagcgcgatt gaatgtatcg
     1081 ccaaagacgg caaccaaaac caagtcggcg tttgggctaa tccgatgaat acggttgaag
     1141 agctgtatct ggccaaaaaa tttgccgacg gcttgggtgt taaaaacttt gcaacccgtt
     1201 tgcgccaaca agacaaacgt ctttcagacg gccttaaagg tgcgcaatgg ttgggacaaa
     1261 gtattgaatc tttggctgac aacgatgccg tattggtagt cggtgcgaac ttgcgcaaag
     1321 aacagccgct cctgaccgcc cgcctgcgcc gcgccgccaa agaccgtatg gccttgagcg
     1381 tattggccgg cagtaaagaa gaattgttta tgccgcttct gtctcaagaa gccgcacatc
     1441 ccgacgagtg ggcaggccgt ctgaaaaacc tgtctgccga tgcggaacat gccgttaccg
     1501 ccagcctgaa aaacgctgaa aaagcagcgg tgattttggg tgcggaagtg caaaaccatc
     1561 ctgattacgc cgccatttat gccgccgcgc aagagttggc ggacgcgacc ggcgcagtgc
     1621 tgggcatttt gccgcaagcc gccaacagcg tcggtgcgga cgtattgggt gtgaactcgg
     1681 gcgagagcgt tgccgaaatg gcaaacacgc cgaaacaggc agtcttgctg ctcaacgtcg
     1741 agcctgaaat cgatacggtt gacggtgcaa aagccgtagc cgcgttgaaa caggcgaaaa
     1801 gcgtgatggc gtttacgccg tttgtcagcg aaacgctgct ggacgtgtgc gacgtattgc
     1861 tgccgattgc gccgtttacc gaaacatcag gcagcttcat caatatggaa ggccgtctgc
     1921 aatctttcca cggcgtggta caaggcttcg gcgattcacg tccgttgtgg aaagtgttgc
     1981 gcgtattggg caacctgttt gacctgaaag gttttgaata ccacgatacc gccgcgattc
     2041 tgaaagacgc gctggatgcg gaaagcctgc cgtccaaact ggacaaccgc agcacatggg
     2101 caggggaggg cgttcagacg gcctcaaacc gcctcgtccg tgtcggcggc gtcggtattt
     2161 atcatactga ttctatcgtg cgccgttccg caccgttgca agaaaccagc catgccgccg
     2221 tgcctgccgc gcgtgtaaat ccgaacacat tggcacgctt gggcctgcaa gacggacaaa
     2281 ccgctgtcgc caaacaaaac ggcgcaagcg tatcggttgc cgtcaaagcc gatgccggtt
     2341 tgcctgaaaa cgtggtgcat ctgccgctgc ataccgaaaa tgccgcgctg ggtgcgttga
     2401 tgggcactat tgaactggcg ggagcttgat tatgcaggaa tggttccaaa acctctttgc
     2461 cgcaacgctc ggtctgggcg atttgggcat caccgtaggc ttggtggtat ccgtcatcgt
     2521 caaaatcgtg attatcctga ttccgctgat tctgaccgtt gcctacctga cgtatttcga
     2581 acgtaaagtc atcggcttta tgcagcttcg cgtcggcccg aacgtaaccg gcccgcgggg
     2641 tctgattcag ccgtttgccg acgtgttcaa actcttgttt aaagaggtaa cccgtccgaa
     2701 gctgtcaaac aaagccttat tctacatcgg cccgattatg tcgctcgccc cgtctttcgc
     2761 ggcgtgggcg gtgattccgt ttaacgaaga atgggtgctg accaatatca atatcggcct
     2821 tttgtacatc ctgatgatta cctcgctgtc ggtttacggc gtgattatcg cgggctgggc
     2881 ttccaactcc aaatattcgt tcttgggtgc aatgcgtgct tccgcgcaaa gcatttccta
     2941 cgagattgcc atgagtgccg cgctggtgtg cgttgtgatg gtatcgggca gcatgaactt
     3001 ctccgacatc gttgccgcgc aggcaaaagg catcgcgggc ggttctgtgt tctcgtggaa
     3061 ctggctgccg ctgttcccaa tcttcatcgt ctatctgatt tccgccgttg ccgaaaccaa
     3121 ccgcgcaccg tttgacgtgg cagagggcga gtctgaaatc gttgccggac accacgtcga
     3181 atactccggc tttgcattcg cgctgttctt ccttgccgaa tacattttca tgattctgat
     3241 tgccgcgctg acatcgctga tgttcctcgg cggctggttg tctccgttcc cgcaaagctg
     3301 gggcattgtc ggtacgcctt ccgcattctg gatgttcgtg aaaatggcgg cggtgctgta
     3361 ctggtatctg tggattcgtg caaccttccc acgctaccgc tacgaccaaa tcatgcgttt
     3421 gggctggaaa gtgctgattc cgatcggctt cgcctacatc gtggttttgg gcgtgtggat
     3481 gatttcaccg ctgaatttgt ggaaataagt ttcagacggc atcttgaggc cgtctgaaca
     3541 aagcgatttt gaatacctaa cgaaatccct gttttgaggg aacataatat ggctaactta
     3601 gtaaaaacct ttctgcttgg cgaactggtc aaaggcatgg gcgtaacgct caaaaacttt
     3661 tttgcccgca aagacacaat ttattttccc gaagagaaaa cgccgcaatc cgtgcgtttc
     3721 cgcggtctgc acgcgcaacg ccgttatccg aacggagagg aacgctgcat cgcgtgtaaa
     3781 ttgtgcgagg cggtgtgtcc ggcaatggcg atcaacatcg aatcggaaga acgcgaagac
     3841 ggcacccgcc gcaccaagcg ttacgacatc gacctgacca agtgcatctt ctgcggtttc
     3901 tgcgaagagg cctgcccgac cgatgcgatt gtggaaaccc atatttttga ataccacggc
     3961 gagaaaaaag gcgacttgca tatgaccaag cccatcctct tggcaatcgg cgacaaatac
     4021 gaagccgaaa tcgccaaacg caaagccgct gacgcgccgt accgctaagg agcagacgaa
     4081 tgactttttc cgtgattctg ttctatatcc ttgccgccat cgttttgtac ggtgcggttc
     4141 gtaccgttac cgccaaaaac cctgtccatg ccgctttgca tctggtgctg accttctgcg
     4201 tgagcgcgat gatttggatg ctgatgcagg cggagttttt gggcgtgacg ctggtggtgg
     4261 tttacgtcgg cgccgtaatg gtgttgttcc tgttcgtcgt gatgatgctg aatatcgaca
     4321 tcgaggaaat gcgcgccggt ttctggcggc acgcgcctgt tgccggtgtg gtcggtacgt
     4381 tgttggcggt tgccctgatc ctgattctgg tcaacccgaa aaccgacctg gccgcatttg
     4441 gtctgatgaa agacattccg gccgattaca acaatatccg cgatttgggc agccgtattt
     4501 ataccgacta cctgttgccg tttgaattgg cggcggtatt gctgctgttg ggcatggtgg
     4561 ccgcgattgc attggttcac cgcaaaacca ccaatccgaa acgtatggat ccagccgacc
     4621 aagttaaagt acgcgccgat caaggccgta tgcgtctggt gaaaatggaa gcggtcaaac
     4681 cgcaagtcga atctgccgaa gaaagcgaag tttcagacgg cctcaagacg gaaggggagg
     4741 gcaaagcatg attactttga cgcattatct ggtattgggc gctctcctgt tcggtatcag
     4801 cgcgatgggc atctttatga accgcaaaaa cgtgctggta ttgctgatgt ccatcgagct
     4861 gatgcttttg gcggtgaact tcaactttat cgccttctcg caacatttgg gcgatactgc
     4921 cggacaaatt ttcgtattct tcgtattgac cgttgccgct gccgaatctg ccatcggttt
     4981 ggcgattatg gtgctggtgt accgcaaccg acaaacgatt aatgttgccg atttggacga
     5041 gttgaaaggg taatcatgaa cgatatgact ttatatttga taattgccct tgttccgttg
     5101 gcaggctcgc tgattgcggg tttgttcggc aacaaaatcg ggcgtgccgg cgcgcatacg
     5161 gttacgatac tcggtgtcgc tgtatctgcc gtgctgtcgg cttatgtgct gtggggattc
     5221 ctcaatggca gccgtaccaa gtttgacgaa aacgtctata cctggctgac aatgggcggc
     5281 ttggattttt ccgtcggctt cttggtcgat acgatgacgg cgatgatgat ggtcgtggta
     5341 acgggcgtgt cgttgatggt gcatatctat accatcggtt atatgcacga tgaaaaagtc
     5401 ggctaccaac gcttcttcag ctatatttct ttgtttacat tcagcatgtt gatgctgatt
     5461 atgagcaaca acttcatcca gctcttcttc ggttgggaag cggtgggctt ggtgtcgtat
     5521 ctcttgatcg gtttctattt caaacgcccg ggcgcgacat ttgccaacct gaaagccttt
     5581 ttgatcaacc gtgtcggcga cttcggcttt ttgctcggta tcggcttggt gcttgcctat
     5641 ttcggcggca gcctgcgcta tcaggacgta ttcgcctatc tgcccaacgt acaaaatgcc
     5701 accatccaac tgtttcccgg tgtggaatgg tctttgatta ccgtaacctg tttgctcctg
     5761 tttgtcggtg cgatgggtaa atcggctcag ttcccgctgc atgtctggct gcccgattcg
     5821 atggaaggcc cgacgccgat ttccgcattg attcacgccg caacgatggt tacggcgggt
     5881 ctgttcatgg tgtcgcgtat gtcgccgatt tacgaaatga gcagcactgc gctgtcggtc
     5941 attatggtta tcggcgcgat taccgccctg tttatgggct tcttgggtgt gattcaaaac
     6001 gacatcaaac gtgtggttgc gtattctacc ctgtcgcaac tgggctacat gaccgtggct
     6061 ctgggcgcgt ccgcctattc tgtggcgatg ttccacgtca tgacccacgc cttctttaaa
     6121 gccttgctgt tcttggcggc gggcagtgcg attatcggta tgcaccacga tcaagatatg
     6181 cgccatatgg gcaacctgaa aaaatacatg ccgattactt ggctgactat gctgatcggc
     6241 aatttgtctc tgatcggtac gccgttcttc tccggcttct actccaaaga ttcgattatc
     6301 gaagcagtga aatacagcac cctgccgggc agcggcgttg cctattttgc cgtcctcgcc
     6361 agcgtgttcg ttaccgcgtt ttacgcgttc cgccaatact ttatggtgtt ccacggcgaa
     6421 gaaaaatggc gcagcctgcc cgaacaccat tcagacggcc atggtgaaga acatcacggc
     6481 ttgggcaaaa acgacaatcc gcacgaaagc ccgctggtcg ttaccctgcc gctgattctg
     6541 cttgcgattc cgtccgtcat catcggctac atcgccatcg aacccatgct ctacggcgat
     6601 ttcttcaaag acgtgatttt cgtcaacgcc gacgcgcatc cgaccatgca catcatgaaa
     6661 gaagagttcc acggcgcatt ggcgatggtg tcgcacagtc tgcattcgcc cgtcctctat
     6721 ctcgctaccg caggcgtatt gagcgcatgg cttttgtacg tcaaactgcc gcacctgccc
     6781 gctaaaatcg cgcaggcgtt ccgtccggtt tacgttttgt ttgaaaacaa atactacctt
     6841 gacgccttgt atttcaacgt cttcgccaaa ggcacgcgcg cattgggaaa cttcttctgg
     6901 aaagtcggcg ataccgccat tatcgacaac ggcatcgtca acggctccgc caaactggtc
     6961 ggcgcaattg ccgcgcaagt gcgtaaagtc caaaccggct ttatctacac ctacgccgcc
     7021 gctatggtgt tcggcgtatt ggtattgctc ggcatgacct tctggggatt gttccgataa
     7081 ggatgaggct tcagacggcc tgcttatcgc tggtgtagag gtcgtctgaa aagaaggttt
     7141 caggcggcaa tagattagag tttcagacga catctgacat ttcagatagt caggccgtct
     7201 gaaaaataga agggcggata atatggataa acggctgttt gacggtacgg ttcgttttga
     7261 gcatgtaacc ggtgtcggca cgatgcaggt agatttccag cccaatcagc gtgtttatac
     7321 gatgattggc gagaatggtg tcggtaagac caaatttttg gaatgtttgt ttacggtatt
     7381 gttgtttact tatcgaatgg agaacataaa cataggtttt agttggatag atttgcaacg
     7441 tttacctttg aaaatagttg aattgcctaa tggaaaaaat gaggatttta ggcgcttttc
     7501 agagcgtagt aatcagggct ttaatattaa cgaagacgtt ttgttctcag gatatcaaca
     7561 tcgacttcct tttgtatatt tgccggcaca gagtcgtggg tgggtaaaaa aaacaaactt
     7621 ccaaactcag tcgctgggaa gttctcaagg gcgtgcaagc aaatatctgg ggaatttgtt
     7681 ttctgttttt caatctggcc atagttcatt ctcgcttaaa tccttaaata tggaaaacga
     7741 tttagaagaa tggattatcc agcgcgcccg atcagccaat ccgtatcagg caaacgaaga
     7801 taatcgaaaa attgaaattg atactttact gtttcttctt aatcaatttg atggtcgtat
     7861 tgcacctgat tttttagaaa ttaaaggaga tgatcgtata ttcatcaagt tggataatca
     7921 aaaacgcgaa ttgtcaggat tatccagcgg gtttacgtct attttgaaaa ttatgcaatc
     7981 tattgttgcg ggatacagtt actttaccaa tgagcagcaa attgccaatg tgcgcggcgt
     8041 ggtgctgatt gacgaaatag aaagccattt gcacaacgaa tggcagatca aaatcgtgcc
     8101 gctgctgaaa aaattgttcc cgaacacaac gtttttcatt accacgcatt cttctttagt
     8161 catcagtcag ttagagcagg gagggcctat cgtttggtac gtagcaagga ggacggtgta
     8221 gtctatggca agatgattga ctatccaagt aatgcttctt ttgtggattt gctcaacgaa
     8281 gcattcggcg tggatttgaa taagaagaag atagaacgcg cccaagaacg taatcaagaa
     8341 caggcaaaaa agccttattg gaattggtac gtcaggaatt gggcggatta ggagataaat
     8401 aatgacggtt tatcttttag ataccaatta cttggtttat ttggcggatg atgacagtga
     8461 cgaagaaaag cggaaagccg tattgagcga tatggcggaa aagttgcagc aggatgacaa
     8521 ccgttttgtg attaccccgc ttatccgtta tgaagttttg cgcggtgtgg attggggaaa
     8581 atctgaaaaa ttgtctcgat tgaccggtgt tttggcacag ttttagtctt tggacattac
     8641 acagaatgtt tctgatttgg caagtaatct gtaccgtttt gataagtttg aagccgaacg
     8701 tgataacacg ccgaagaatt tagaaaaacg taaattcgat atgtttcatt atgcgactgc
     8761 cagtgtgaat aatttagaaa tcttatccca cgataccgat gttaataaga ttaaagattt
     8821 acatgaaaga atgaggctgg aagattcggc agaattagct tgagacaaat agcagaaacc
     8881 tgaacaaacc tttttcagac ggcctcttaa aacgtcgtct gacaaaccaa cccgtacaac
     8941 gatattttta ttttaaccac aggttaacca ctatgttttc caattaccta ctcagcttgg
     9001 caatatggat acccatcgcc gcaggcgtgc tggttttggc gaccggtaaa gacagccgtg
     9061 cgccgctggc gcgtgtgctt gccttcatgg gtgcgcttgc cggtttcttg gtaacgttgc
     9121 ccctgtttac cggtttcgac cgtttgagcg gcggctatca atttaccgag ttccacgagt
     9181 ggattccgct tctgaaaatc aactacgcat tgggcgtgga cggtatttca gtgctcttta
     9241 tcatcttgaa tgcgtttatt acgctgctgg tggtattggc cggttgggaa gtcattcaga
     9301 aacgtccggc gcagtatatg gcggcattcc tgatgatgtc gggtttgatt aacggcgcgt
     9361 ttgccgcgca ggatgcgatt ctgttttatg tgttcttcga gggtatgctg attccgctgt
     9421 acctgattat cggtgtatgg ggcggcccgc gccgcgtcta tgcgtcggtc aagctgttcc
     9481 tctatacgct gacgggttcg ctcttgatgt tggttgccat ggtttacctg tactatcaga
     9541 caggcagctt ctctattgtc gatttccaaa acatcaaaca gattccgttg ggcgtacaac
     9601 agcttttgtt tgtagcgttc ttcctgtcat ttgccgtaaa agtgccgatg ttccccgtac
     9661 acacttggct gccggatgcc cacgttgaag cgccgaccgg cggttcgatg gtgttggcgg
     9721 caattacgct gaaactgggt gcgtatggtt tcttgcgctt tatcctgccg attatgccgg
     9781 acgcggctcg ctattttgcc cccgtgatca tcgtattgag cctgattgcc gtaatttata
     9841 tcggtatggt ggccctggtg caaaccgata tgaagaaact ggtggcgtat tcgtccatca
     9901 gccatatggg ttttgtaacg cttgggatgt ttttgtttgt tgacgggcag ttggacgact
     9961 gggcgttgaa aggcgcggtc attcagatga tttcgcacgg cttcgtgtcg gcggcgatgt
    10021 ttatgtgcat cggtgtgatg tacgaccgcc tgcacacgcg caatattgcc gattacggcg
    10081 gcgtggtcaa tgtgatgccc aagtttgcgg cgtttatgat gttgttcggt atggcgaacg
    10141 ccggcctgcc tgcgacttcc ggcttcgtgg gcgagtttat ggtggttatg ggcgcggtta
    10201 aagtgaattt ctgggtgggc gcgctggcgg ctatgacgct gatttacggc gcgtcttata
    10261 ccctgtggat gtacaagcgc gtgattttcg gcgcgattca caatccgcac gttgccgaaa
    10321 tgaaagacat caattgccgc gagtttgcga ttctggcggt tttggcggtt gctgttttgg
    10381 ggatgggcct gtatccgaac gcatttatcg aagtggtgca tcaggcggca aacgatttga
    10441 ttgcccatgt ggcgcaaagc aagatttgag gtgtgtaaat gaattggtca gatttgaatt
    10501 taatgcccgc actgcccgaa gtcgcgctgc tgtcgctact ggtgttattg ttgccggcgg
    10561 acttgtgggc cagtgatgac aaatgccgtt ggacgcatta cggcgcgttg gcaacggtgg
    10621 cggttacggc ggcggtgcag cttgccgtgt gggaacaggg cagcacgtct tcgttcaacg
    10681 ggatgtatat tgcggacggt atgtcgcgtt tggcaaaaat ggttttatat gccttgacgt
    10741 ttgtcctgtt tgtctatgcc aagccctaca accaagtgcg cggtattttc aaaggcgagt
    10801 tttacaccct gtcgctgttt gccctcttgg gcatgagcgt gatggtgagc gcggggcatt
    10861 ttttaactgc ctatatcggt ttggaactct tgtcgctcgc cctttacgcc ctgattgccc
    10921 tgcgccgcga ttccggtttt gccgccgaag ccgccttgaa atattttgtt ctgggcgcgc
    10981 tggcatccgg cctgctgctc tacggtattt ctatggttta cggcgcaacc ggttcgctgg
    11041 aatttgccgg cgtgctcgcc tcttccttca atgaagaagc taacgaatgg ctgttgaaac
    11101 tgggtctggt gtttatcgtc gtcgccgttg cgttcaaact cggcgcggta ccgttccata
    11161 tgtggatgcc cgacgtgtat cacggcgcgc ccacttctgt taccgccttg gtcggcactg
    11221 ccccgaaaat cgccgccgtc gttttcgctt tccgcatcct cgttaccggg ctgggaaccg
    11281 tgcatcatga ctggtctctg atgtttgccc tgcttgccgc cgcctcgctg ctggtcggca
    11341 accttgccgc catcatgcag accaatatca aacgtatgct cgcctattcc accgtatcgc
    11401 atacgggttt catcctgttg gcgtttatgg cgggcgcggt cggctttgcg gcgggcctct
    11461 attacgccat tacctacgcg ctgatggcgg cggcagggtt cggcgtgttg atggtgttgt
    11521 cggacgggga caacgagtgc gaaaacatca gcgatttggc agggttgaac caacaccgcg
    11581 tatggcttgc ctttttgatg ctgctggtta tgttctctat ggcgggcatt ccgccgctga
    11641 tgggttttta cgccaaattc ggcgtgatta tggcgctttt gaaacaaggc tatgtatggt
    11701 tgtcggtgtt tgccgtcgtg atgtcgctgg ttggcgcgtt ctactacctg cgcgtggtca
    11761 aagtcatgta ttttgacgaa tccggccgcg cccgtcccgc cgccggcggc aacaatgccg
    11821 caaaatccct cttaagcgtc aacgcgctgc tgttggtgct gtggggcatt atgccgcaaa
    11881 ccgttatcga ctggtgcgcc aaggcgttgg agaacacgct gtgagccgcc gaaacggcag
    11941 ccgtgtcaga ggctgccgtt tttgttaaga tatgccgttc cgcaatgcag ttcagacggc
    12001 atcgccgccg acaatgccca aacagaaagc ccatcatgac cgcatccatg tacatccttt
    12061 tggtcttggc actcatcttt gccaacgccc ccttcctcac gaccagactg ttcggcgtgg
    12121 ccgcgctcaa gcgcaaacat ttcggacacc acctgatcga gctggcggca ggtttcgcgc
    12181 tgaccgcctc tcttgcctac atcctcgaat cccgtgcggg agcggtacac aatcagggtt
    12241 gggagtttta cgccaccgtc gtctgcctgt acctcatttt cgccttcccg tgtttcgtgc
    12301 ggcggtattt ttggcacacg cgcaacaggg aataaacaag caaacaagca taggaatgcc
    12361 gtctgaaacc ctttcaggcg gcatttgttt cattcaagtg caggccggca tcgctgtgcc
    12421 ggcacgtttc agtcggcgat atacgccggt tttaatattt gcgtgcgact gcaaattctg
    12481 ccaactgccg caggcgcagg gctttgtcgc cgaagggttc gagcagcgcg actgcttcgg
    12541 caaccagttt gtgtgcgtat gagcgcgccg cttccaagcc catcagtttc acataagtcg
    12601 gcttgtcgtt gtcggcatct ttgcccgccg tcttgcccaa agtcgccgtg tccgcttcac
    12661 aatccaacac atcgtcgatg acttggaacg ccaaacccag ttttgccgcg taagcgtcca
    12721 atacggcaag ttccgcgtcg gacagatcgg gacacgccgt cgcccccaat aaaaccgccg
    12781 cacggatcaa cgcccccgtt ttcaggctgt gcatccgttc caaatcggct tgaaccattt
    12841 gtttgccgac atttgccaaa tcgattgcct gtccgcccgc catacccgcg ctgccgcccg
    12901 cttttgccaa caccgacaac attgccaact ggcgtgcggc gggcagttct gtcggacggc
    12961 tcaacacgtc gaaggcttgg gtttgtaaag cgtcgccggt cagaagggcg gtggcttcgc
    13021 catatttgat gtggcaggtc ggtttgccgc gccgcaggct gtcgttgtcc atcgccggca
    13081 tatcgtcgtg aaccaaagaa tagacgtgga tcatttcgat tgccgccatt gcctgtccga
    13141 ccgcatccgc cacggccccg cccaattccg aagccgccag caccagcatc gggcgcagac
    13201 gcttgccgcc gtccaaagcc gcatagcgca tcgcttcgtg cagcgtgtgc gggatttcgt
    13261 ttccagacgg caaaaagcgt tccagcagca gctctgtctg cgcctgcgcc ctctgttgcc
    13321 acgctttcaa atcattcgtc ggattcaagg ttcaactcct tcgtcccgtc cgcatccaaa
    13381 acctgtaatt tctgttcgac ttgggccagc ttggtttggc agtacctgac cagctcgttg
    13441 ccttcctgat aggcggcaag cgcgtcttcc aagggcattt cgccctgcat ggactgcgtc
    13501 agcgattcaa ggcgcgacaa ggcctcttca aacgatttcg gggtgttttt cttcatagtt
    13561 tcgggtgttc cggtaaaaag cggattgtag catttttgca aaacggatgc cgtctgaaac
    13621 ccgaatccgg cttcagacgg cattttttcc gccccggtcg gcaaggcgtt acccgagcag
    13681 ttcgtcggtg atgccctgca aaaaggcgag gcgttcgggg cttgccgccc cggtttcggc
    13741 ggcggctttg aaggcgcagc cgggttcggc gcggtgggtg cagttgtgga agcggcattg
    13801 cccgacaagg tggcggaaat cggggaaata gtgcggcaaa tcggcggctt ggaggtggtg
    13861 taaaccaaat tcttgcaaac ccggggagtc gatgagttgg gtttcgccgt tcaaatcata
    13921 aagccgggcg tgggtggtgg tgtgttttcc cgagtcgagt gcggcggaaa tgtcgccggt
    13981 gcgggcggtt tggctgccta aaagggcgtt ggtcagggtg gatttgccca taccgctttg
    14041 tccgagcagg atgttgctgt gcccttgcag gacggggcgc aggctgtcgg cgttttccag
    14101 cacgcgggtt tcgatgacgg ggtaacccag cgtttcgtag aatttgagtt tttcgagcca
    14161 gagggcggtt tcgggcaggt cggctttgtt caggacgatg acggcgcgga tgccggcggc
    14221 ttcggcggca agcagggcgc gctgtagcag ccgcacgctc ggactcggga cggcggcggt
    14281 tacgatgagg agttgggtaa cgttggcggc gatgagtttg gttttccacg cgtcttggcg
    14341 gtagagcagg ctttggcgcg gtaaaaaatc ttcaatcaca acttgttcgg cgttgacggg
    14401 gctgatgcgg acgcggtcgc cgcaggcgaa atcgacgcgt tttttgcggg tgctggcttc
    14461 gtaggttgtg ccgtcgggcg tgcggacgat gtagcggcgg ccgtagccgg cggtaatttg
    14521 ggcggtgtcg ttcatggttt ctttggggtt gggtgtggaa atgccgtctg aaaaggggtg
    14581 ttcggacggc atcggttcag tcgtgctgcc actcgacgtg ttcgctgagg aagccgccgc
    14641 tctggtgcgc ccagagtttg gcgtaaagcc cgcgtttttc gaggagttcg gcgtgtgtgc
    14701 cttcttcgat gatgcggcct ttgtcgagga cgacgagcct gtccatcgcg gcaatggtgg
    14761 agaggcggtg ggcgatggcg atgacggttt tgccttccat cattttgtcg aggctttctt
    14821 ggatggcggc ttcaacttcg gaatcgagcg cgctggtggc ttcgtcaagc agcaggatgg
    14881 gtgcgtcttt gagcatcacg cgggcgatgg cgatgcgctg gcgttgcccg ccggagagtt
    14941 tcacgccgcg ttcgccgacg tgcgcgtcgt agccgcgccg ccctttggca tcggaaaggt
    15001 cggggatgaa gccggcggct tcggcgcgtt cggcggcgga aaccatttcg gcatcggtcg
    15061 cgtcggggcg gccgtaaata atgttgtcgc gcacggaacg gtgcagcagc gaggtatctt
    15121 gtgtgaccaa accgatttgg gcgcgtaaag attcttgggt aacgccgctt atgtcctgcc
    15181 cgtcgatcga aaccgtgccg ctttgcggtt cgtagaaacg caaaagcagg ttgacgatgg
    15241 tggatttgcc cgcgccgctg cgtccgatca agccgacttt ttcgccgggt ttgatattca
    15301 ggttaaagcc gttgagcagc ggtttgccgg cttcgtagca aaaatcgacg tgttcgaact
    15361 tgattgcgcc ttgcggcacg ttcagcggca gggcttgggg cttgtcgagg atggtgtgcg
    15421 gtttggacag ggtcgccatg ccgtcgttga cgatgccgat gttttcaaac agccgcgcgg
    15481 attcccacat aatgtattgc gacagcccgt tgacgcgcaa cgccatcgcg gtggcggtgg
    15541 cgaccgcacc cacgccgacc tgtccgttgt gccaaagcca gatgcccagt gcggcggtgg
    15601 agagggtcag cgaagtgttg acgatgaagc tgcacgaatg cagcagagtc gccagccgca
    15661 tttgggcgcg caccgtaacc ataaattctt ccatcgactg cttggcatag acggcttcgc
    15721 gcgcgccgtg gaaaaagagt ttgacggtgg cgatattgga ataggcatcg gtaatgcggc
    15781 cggtcatcag cgagcgggca ttcgcctgcc gtgcggcggt ttgccccaat ctgggaatca
    15841 gcaggcgcat taccgaagcg aaaccgatta tccagccgat aaagggcagc agcagccatg
    15901 agtcgagcga ggcgagaatc acgcccgaag tgatgaaata caccgacaca taaacgacca
    15961 tatcggcaac cgtcatcacc gcgtcgcgca acgccagcgc ggtctgcatg actttggcgg
    16021 acacgcggcc ggcaaattcg tcctgataaa aaccgaggct ctggttcagc atcaggcggt
    16081 ggaagttcca gcgcaggcgc atagggaaca cgccctgaag ggtttgcagg cgcacgttgg
    16141 acgcggcaaa cgcccacacg accgaaaacg ccatcatcgc cgccattgcc gccagttccc
    16201 aacctttttc ggcaaacagt tcggcgggcg cgtatttgcc gagccactct acgattttgc
    16261 ccataaattg aaaaatcagg gcttccataa tgccgatgcc ggcggtcagc gcagctaggg
    16321 cggctatcca tttccgcacc ccgtccatac tgctccagac aaaccggcac aagccttttt
    16381 cgggcgtttt cggcgcggct tcggggtagg ggtcgattcg ggactcgaac caggaaaata
    16441 ttttgttcaa cattgttttc gatttcggta aaacagtttc agacggcatc aaacacaatg
    16501 ccgtccgaaa ggagggacaa taacgccatt ttacgggaaa agccgtcggg aagacagcgc
    16561 ggggcggaaa cgcagggttt cgtcagggca aacgccgcgc cgccttcgga cggcattatt
    16621 tcagcaggtt tttcagggca aggcgcacgc cttcgcccac gtccgtcccc ttcggaacgc
    16681 ctttgaccgc cgcttttgcc tcgcgttcgt tgtaacccag cgcaagcagc gtgctgacga
    16741 tgtcttccgt ttcgtcggcg gcgggtgagg cggcaaacag cccgtccgtt accgtatgcg
    16801 cgaccagctt gccgcgcagt tccaagacca tacgttcggc ggtttttttg ccgattcccg
    16861 gagcggagga gaggcgtttg acatcttctt ctgcaaccgc ccgcgccagc tcgtctgccg
    16921 tcatcgccga caaaatgccc aaagccgttt tcgcgccgat gccgccgacc ttaatcagtt
    16981 ggcggaaggt cttgcgttct tccgcagtgg caaaaccaaa taaaagatgt gcgtcttccc
    17041 gaatgataag ctgggtaaac agttgtacgc tttcacccac gggcggcagg ttgtagaagg
    17101 tctgcatcga tacgtcggct tcataaccga caccgttgac atcgatgacg atttgcggag
    17161 ggtttttttc aaccagtttg ccggtcagtc tgctgatcat gtgtgccgaa tcctgaagtg
    17221 tcgggtgcaa aatgccgtct gaaaccggtt cgggctccag acggcacgga ttgtatcaaa
    17281 ttcagtcgtc gcggcgggag gaaatcacgc ggccggtacg ggcatcgaca acgactttgt
    17341 attcctgtcc gtttttgacg atttcgacat catagtgcgg acggccgtcg tcgtgttcga
    17401 gatcgatgtc ggtgattttg ccgccgacac gcgcccaagc cgctttttcg gcttgggcgc
    17461 ggctgatgat tttgtcttgt ttgccgtgtt ggtgtgcggc gtgtccgtgg tcgtcatcgc
    17521 cgtgtccgtc gtggtgggcg agtgcggggg cggaaatgcc caacagtgcg gttgcggcgg
    17581 cggtcaagag aaggtgtttg atgttcatat ttgcccttgt aaatcagggg ttgaaaaatg
    17641 gggatattaa taaggtatcg aataaccgtc aaccggaggt cagtaccgcc cgaaccatac
    17701 cgcgtgcctg cgcttcggct tcggcggcgc gttcctgcga ggcaaacggg cccattttga
    17761 caacgtattc gtaacggcgt ttttcaaccg agaggttcgg gcttgatgac gaagcggcga
    17821 aattttgggc ggcttggttc agataggctt gtgcttcgtg ttccgtaccg aaagatttca
    17881 agtcgataaa gatgtctttg ttttcggcaa ccggtgcgga ttggcccggg acgatttgtt
    17941 cgattttgac gtgtgccgtc ccttggctga caaagcccaa tttttgcgcg gcggctttgg
    18001 atacgtcgat gatgcggttg ccgtggaagg ggccgcggtc gttgacgcgg acgatgacgc
    18061 ttttgccgtt tttggtgttg gttacgcgca catggctggg gatgggcagg gttttgtggg
    18121 cggcggtaaa ggcgttcata tcgtagcggt ctccgccgga agttttgcgc ccgtgaaacc
    18181 tgccgccgta ccacgaggcg ttgccggttt gcgtgaattc ggcgactttg tagctgcggt
    18241 tggcggaggc gtgcagtttt tccggcttga ccactgcgtc ggcggatgcc gtctgaaggg
    18301 agtgtgtgcc gaacgcggcg gtgaggagga aaagggtttt tcgggttaaa gtcaaaatgt
    18361 gttccgttct tgagttgaag acggatgggc attatgccct acggacgcgt tccgaaccgc
    18421 cgcacgctgc ggcagcggtt cggaatgtgt ccgaacggtt ttcagacggc atgaacctgc
    18481 gttcaaacgc cgcctgcgta accgtgttgc cgccacgctt caaagagaat cacggagacg
    18541 gtgttggaaa ggttcatact ccggctgccg ggccgcatcg gcaggcggat tttttgcgcg
    18601 gcgggcaggc tgtcgaggat gtcggcaggc agcccgcgcg tttccggccc gaacagtaaa
    18661 acgtcgcctt tttgaaacgc ggtttcatcg gggcgcgccg tgcctttggt ggtcagggcg
    18721 aaaatacgtc ggcctgccag cgacttgagg cagtcgtcga agttttcgtg caccgtcagg
    18781 ctggcgaatt cgtggtagtc gagcccggcg cgtttcattt tggcggaatc caatgggaag
    18841 ccgagcggtt tgacaaggtg caaatccgcg ccggtattgg cgcacaggcg gatgatgttg
    18901 cccgtgttcg gggggatttc cggctgatat aaaacgatgg taagcataaa tatcaatcac
    18961 ttataggcgc gtaaccttgc cataaggcgg atggggtgtc aaaaaattta gttatttttt
    19021 cattggcgtg cgtgccagcg tccagcagca gattcggttt gcgcccgatt ttttcagcgt
    19081 ctttgccaat tcgtccagcg tcgcgccggt ggtaaagaca tcgtcgatta acaaaatatt
    19141 acagttttcc ggtatcggtg tgcggatttc aaaggcgttt ttgatgtttc gccgccgttc
    19201 gccgcctttg agcgtgcttt gcggcgggcg gtggtgtcgg aatacggtgt gtcggggcag
    19261 tatctgccag ccgtagcgtt gcgccaacaa cccgacgatg ctctcgcttt ggttgaaccc
    19321 gcgttgcagc agccgctccc tgcttagcgg aacgggtagg acgaaatcga aacattcgtc
    19381 tgaaagccgg tcgggcggat tctgcatcgt caggtctgcc agcggctgcg ccatgcccaa
    19441 atcagccaag tgcttcagcg cgcgtatcat attgctgacg ggcggttcgt aatgcagcga
    19501 tgcccacatc cggtcgaatg cgggcggttt tttctgacat ccgccgcaca ccgcgccgcc
    19561 ttgaacgtgc ctgaaacaca gggggcagct gtttgccgcg tcggtgcgga aacttgccaa
    19621 atcggtattg cagccggcgc agatgccgtc tgaaacgccg gacgaaccgt ggcataatac
    19681 gcaacgcctg atagtgggcg cgtctgcgat gcgccgccaa caagagagaa aattcatgcc
    19741 tgatgctgcc aaaaaagttt acctgataca cggttggggg gcgaaccgcc acgcgttcga
    19801 cgatttgatg ccgcgcctgc ccgcaacgtg gccggtgtcc gccgtcgatt tgcccggaca
    19861 cggggacgcg cccttcgcgc aacccttcga tattgaggca gctgccgacg gcattgccgc
    19921 tcaaatcgat acgtcggccg acattctcgg ctggtcactc ggcggattgg tcgcgctgta
    19981 tctggcggcg cgccatcccg acaaagtccg ttcgctctgc ctgacggcga gtttcgcacg
    20041 gctgacggct gccgaagact atcccgaagg gcttgccgcg cctgcactgg gcaaaatggt
    20101 cggcgcgttc cgaacggatt atgccaaaca tatcaaacag tttctacaac tccagcttct
    20161 gcacacgcct gatgccgccg aaatcatcgg cagaatcctg cccgatttgg cgcgctgcgg
    20221 cacgcctcaa gccttacagg aagctttgga cgcggcggaa agggcggatg tgcggcattt
    20281 gttggacaag atagatgttc cggtactgct ggtgttcggc ggcaaagacg cgattacacc
    20341 gccgcgtatg ggcgagtatc tgcatcggca tttgaaagga agcaggctgg ttgtgatgga
    20401 aaaggcggca cacgcgccgt ttttgagcca tgcggaagtg tttgccgcgc tgtaccgcga
    20461 ctttgtcgaa gggggtttga catgaatccg caggacaaat gctggcaggt tcaccgccat
    20521 cttgccgaac ataccgacca acgcctgaca ctcgtccgca acgcgcccgg gcatatcctg
    20581 cttgccggtg cggatgcgga catcagccgc agcctgttgg cgaagcgcta tccgctggcg
    20641 gtatttgaag aatacgattc ccgtgcggat tttttggcgg ctgccgcagc cgcgcgcaag
    20701 ggcggtttct ggcaaaaact gacgggcagg ggcgtggtgc agcactgcca atccccgacc
    20761 gtgccgctgc ccgaagcgtg tgccgatatg ttgtggtcga acctcggact gttggcggcg
    20821 gaacaaatcc ttcccgtgct gcacaactgg gcgcgcgcgc tgaagacgga cgggctgctg
    20881 tttttcacct gcttcgggcg cgataccttg gcggaactga aatcccgtct gaaagaaaac
    20941 ggcattgaaa gccgcagcgt gatgtttccc gatatgcacg acttgggcga tatgctcgcc
    21001 gaaaacggct tttacgatcc agttaccgat acggcgaagc tggtgttgga ctacaaaaag
    21061 gcggaaacgt tttgggcgga tatggacacg ctgggcgttt ggcgggcggt ggcgtgggac
    21121 gacgaaaacg ccgcgcgttc gtgtgtcggg gcgatatttg aacgggaagg cggtttgggc
    21181 attacgctgg aaacggtgta cggacacgcc gtgaaaaaac tggtgctgcc gcagggggag
    21241 aacgtggtgc gtttttttcc gaagagatga tgtgcagatg acgtctgaag ccgtttccaa
    21301 gtttcagacg gcatttgtct gtgaaaaccg acagaaataa aggaaatgcc gatgtatagt
    21361 gaattaaatt taaaccggta cggcgttggc tcgccttgcc gtactatctg tactgtccgc
    21421 ggcttcgccg ccttgtcctg atttaaattt aatccactat gtgccgatgc cggagacgta
    21481 tattgcgtct ataacatcag actgaagcag tacactgcct gccaggttac ccgagttgaa
    21541 gaacacggtg gcaaaaaaac acatgcgacc ctgctggctt tggactggcg gggcaacaaa
    21601 ccgcttgggg cggcggagct ggcggatttg aaaccgcttt acaaagactt tatgtattgg
    21661 gaacgcggtc tgcacatgta taaggcaagt gccgtcgtgc cgacgggata tgtacgggtt
    21721 gggaataccg cgccgctgtg cggcgaagac acgcagcggt atgcctcttt ttggggcgac
    21781 ggctacgacg tgtaccgtca gttgagatgg cggcagatac ccgaaaaaca gagaaaggca
    21841 ttcaaaaaag ccgccaaaag caaaaatacc gtgatgtttg ccggacggga atacggcata
    21901 tccaaacaga atttgagcga tgtttgggat gattttgaag acgcgatgga actgaaggcg
    21961 tttccctgcc tgtcttcgct gtttctgacc aagtggcata aaaatctata tgaatatctg
    22021 gaggaatatc cgtttatcac ccgattgtgt ctggaaaacc acggtcagac cgtgctggat
    22081 ttcagcaata cgcgcataac cgacctttct gtcgatatga ccggcgtgga atcgctttat
    22141 ctgaacgaag gcttggacag cctgaacctg aaaggcgaaa tcaaggaaaa ctgcaaggtc
    22201 tgtaccgccg gaaagggcgc gggtctgatt ttggaagtgg gcaaaagcgt tcccaaagtg
    22261 cggggtttgg aaaacctgac cgccgtcaac gtcatgggca ttgccgattt cgatatgcaa
    22321 aacctgtcgg aaacgtatcc caagctcaaa accatacggc tgtggggcaa accgggcaat
    22381 atcgccaatt tttccgccgt atcgggattt gaggatttgg aagtgttcac tgccgtagat
    22441 ttgttcggtt tcggcgcgga cgacatccct catcccgacc ggctgccgaa gctgcacagg
    22501 ctgtggatga gcagcctgcc cgaagaggcg gcgaaggcag tgaagaaact ttataagaaa
    22561 cggaaggaag acgggctgga cctgtggata gaaaaagccc gaaaacccga atggctggcg
    22621 caaaatttcg acaacccctt ccgcgattgg gacggcgcgg agcatatccc gaaaagccat
    22681 gccaaaaaag cggcggagct gtaccggaaa acccgtgccg gcgtggtcaa actgctcggc
    22741 aatccgcctg aaaacacagg ggaggggctg gcagaggcgg tcaaagccta taccggaggt
    22801 ttcaacaaaa tggacaaaaa acactttatc gacacggtag agcgggaaga tattgccgag
    22861 gcgttggaaa cgatattgga cctgataccg gatgggtctt gtgccgacaa ggaaaaactg
    22921 tttgaaatat ttgataaaaa caggaatttt taaacagagg caatgccgtc tgaaacttgg
    22981 taacgggctt cagacggcat cccgttccaa caccgccaac accgccgcac cgtaacgtgc
    23041 ggctttttct tcgcctacgc cgtatacggc ggcaagctcc gccaaacctt ccggctgttt
    23101 ggcggcaatg gcgcgcaggg cggctttgct gagaatgcgg tagggttcgg actgttcgtg
    23161 ttttgcggtt tcgttgcacc attgaatcag ggcgcgcatc aggcagcgtt tgtgtttggc
    23221 ggtttcatcg atgccgtctg aaaacggacg gcagacggcg aggatgtccc gtccgtattt
    23281 ggcggcgcgt acgctgccca agccgtgtac ggcttcgagg tcggtttcgg tttcgggcgt
    23341 ggcggcaagc atatcggcaa ggctttcgtc ggagaggacg gtatgcaggg cgcagttttc
    23401 cgcccttgcc tgttcatacc gccaggcttc gagtttttga cgcagttgtt gttcgcgttc
    23461 ggtttgcgga cggatgacgg catcgtggct aaagtcggca gcgttttggc agactttgag
    23521 tataccgtgt ccgaaacgtt cggttttggc ttcgcccaag ccgtagatgt cgtgcagccc
    23581 gttgaggttt tgcggcattt tttcgacaag gtcgcgcagg gttttgtcgc cgaaaatcat
    23641 ataggcgggg atgccttcgg cttcagcctg tttcatgcgc caaacgcgca atgcctgcca
    23701 caggcgttct tcgcgttcgg tacgcagcca gttgtctttg agggtgcggg tggcgggctt
    23761 gtcgcgcttg agcgggcgca gcatcacttc ggtttcgcct ttgaggattt ttttggcggc
    23821 ttcggtcagt tgcaatgcct gatatcgggc aatgttgacg gtgaggtagc cgaggctgat
    23881 gcactggcgg atgacgctgc gccattcttt gtcggacaat tccgcgccga tgccgaatgt
    23941 ggacagttgc tcgtgccggt tgccgcgtat ccaatcgtcg cttttgccgc gcaaaaggtt
    24001 ggtgatgtaa ccggcggcaa agcgttgtcc ggcgcggtac acgcagctga gtaatttttg
    24061 caccaacacc gtgccgtcaa accgtacggg cggatgcagg cagttgtcac aatgtccgca
    24121 gggttcggat gcttcgccga aatgtttgag cagcagtacg cggcggcagg cggcggtttc
    24181 gcagacggaa agcatggcat cgagtttttg catttcgatt tgcttttgca cctcgtcgct
    24241 gttgccttcg gcaatccgtt cgcgcagcaa cacccaatcg ttcaagccgt aacacagcca
    24301 actcacggca ggcagcccgt cccggccggc gcgccctgat tcctgataga aatgttcgac
    24361 actctggggc atatcgagat gggcgacaaa gcgcacgtcg ggtttgtcta tgcccatgcc
    24421 gaacgccacg gtcgccacca cgataatatt gtcttcatgc gtaaagcggc gttggttttc
    24481 ctcgcgcacg tccatgctca aaccggcatg atacggaatc gcgtttaatc cgttttcacg
    24541 caaaaactgc gccgcatctt caaccttttt gcggcttagg caatacacaa tgccgctttg
    24601 ccccgtcatt tctttgcgga tgaaatccag caattgtttt ttgccgttgt ttttttcgat
    24661 aacctgataa taaatattcg tgcggtcaaa gctggagaca aattcggacg cgtcgtccaa
    24721 gtgcagataa tgcttgatgt cggcgcgcgt ggcggcatcg gcggtggcgg tcagggcgat
    24781 gcgcgggatg ttcggatagc gttcggcaag cataccgagc tgttgatatt cggggcggaa
    24841 atcgtgtccc caccggctga cgcagtgcgc ttcgtcaatg gcgaacaggc tgacggtttg
    24901 ttggtcgaga aaacgtaaaa agcggtcggt aaccaagcgt tccggcgcga cataaagcag
    24961 cttcagacgg ccttgggcaa gcttgtcggc aatctcgcgc gcctcatctg ccgatgtgcc
    25021 gctgttgact gccgccgctt caatcccggc cacatgcagg ctggccactt ggtcgttcat
    25081 cagcgcaatc agcggcgata cgacaaccgc cacgccttcg cgcatcagcg ccggaatctg
    25141 gtaacacaaa gacttgcccc cgcccgtcgg catcagcacc gtcaaactgc cgccgcctgc
    25201 caaagtattg atgacatcct cctgcctgcc gcgaaattcg ggatagccga atacttcgtg
    25261 caaaatctgt ctggcggtgg gtcggtgcgt cattgttccg tgtccggtaa gggtgttggt
    25321 cggtcggcgg caatatgccg tctgaaatcg ggattttaga agagtttgcc cacttccgcc
    25381 ccaatatcgt cggcacgcat aaacgtttcg ccgatcagga aggtatgcac gccgcgcgcc
    25441 cgcataaatt ccacatccgc cttgcctgta atgccgcttt cggtaacgac ggttttgcct
    25501 tccagcgcgg gcagcagcga cagggtttgg tcgagggaga cttcaaaagt ccgcaggttg
    25561 cggttgttta cgccccgcaa cggcgtggtc aggttgcggc atttttccaa ttcggtttcg
    25621 tcgtgcagct cgagcaggac ggtcatgccc aattcgtgcg ccagcgcctc aaagcgttcc
    25681 aattgtccct gttccagtgc ggcggcaatc agcaggacgg catccgcccc ccatgcgcgc
    25741 gcctgataaa cctggtattc gtcgatgatg aagtctttgc gcagcacggg cagcaatacg
    25801 gcttcgcgcg cctgtttgag gtattcgggc gaaccttgga aatagggttc gtcggtcagt
    25861 accgacaaac acgccgcccc ggcgttttca taggcgcggg caatctcggc agggcggaag
    25921 tcggggcgga ttaacccttt gctcgggctt gcctttttga tttcggcaat gacggcgggc
    25981 aggtttaggc ggtgtttgcc gcgtatcgaa tcgatgaagc tgcggacggg cgcggcttct
    26041 gcggcaagtg cgcggatgtg ttcggcgttg acggcggctt tttgggcggc aacttcctgt
    26101 gccttggtgg caaggatttt attgaggatg tcggtcatgt cgtgttccgt atttgtccgg
    26161 ggaaaggggg aatattagca tcaaaccgtt aacgcctgtt tgtgcggaag ctgtcaaaat
    26221 aggacaggac ggtctgcggc agccattgca ggtgcagcct gccgccggtg ctgctgacaa
    26281 agccggcgtg cccgccgtgt gcaggttgga acagggtaac ggcttcggac gcttcgtccg
    26341 cacggggcag ggcttcgggc ggcaggaagg ggtcgttggc ggcattgagc aggagcagcg
    26401 gtttggcaac gtgtttgagc agcggtttgc aggaagtttg gcggtagtag tcgtgccggt
    26461 cggcaaagcc gtgcagcggt gcggtgaaac ggtcgtcaaa ctcgcccagt gttttgcacc
    26521 ctgcggcaaa tgccgtctga aaaccttgga gcgaacgtgc tttgggtatc agtgtgcgga
    26581 ggaagtagcg cgtgtagagc agccgcgtga tgccgctgtc gaagcggctg cctgccgcct
    26641 ctgcatcaac gggggcggat acggcggccg aggcgtgcgg caatgccttt ttgccctgtt
    26701 cgcccaaata ttttgccggc gcgttgccgc ccagcgatac gccgacggcg tatatttcac
    26761 ggtaacgcgc ggcgagcgtg tccaaagcaa aggcgatttc ggcggtatca cccaagtggt
    26821 agaacaccgg ggcggtgttc gctacgccgc cgcagctgcg gaaatggacg actgcgccgt
    26881 gccaaccccg attgcgtacc gcgagcatca gttcggccgc gtaatggctg cggctgcttc
    26941 cttccaaacc gtgaaacagc acgaccagcg gcgcatcggg cgaaatgccg cctgctgaaa
    27001 agtcgtaggc ggttttggtt ttacccgtgc tgtcgggaag cagctcgcgg cggtatgcgg
    27061 gtgcggggtg ttgcaggaat ttggcggcaa tcgtgtcggc attgccgttg cggaggaaaa
    27121 agggcgtgtc cggcggtgtc aaaatcataa ggtatcggtt tttctgtttt cagacggcat
    27181 tggtgatgcg gcagcccgtc cggccggtgc ggacgcgggc gatgcgcgcc cggatatagg
    27241 cgcggaaaaa gcgttttccg aaaaaggata tcggcatcgg tcagctttcc acgcgtttga
    27301 aatggcgcgg acggaagccc aaagccgcca gagatgcgaa atacagtccg ccgccgacgg
    27361 caatcaggat gcagagctgc cccgctttcc gcattccgcc ggcgtgcgcc cattcgaacg
    27421 gcaggcaagc ctgcgccgcc cacagtccgc cgcacatcac ggcgagcgcg agcagcattt
    27481 tcgccaagaa cgccgcccaa cccctgccgg gccggtaaat accgtgtttg cgcaacagga
    27541 agaacaacaa tccggcgttg atgcacgcgc ccaggccgat ggcgagcgaa agcccggcgt
    27601 gtttcaacgg accgataaag gcgaggttca tcaactgcgt gcagatgagc gtgaagatgg
    27661 cgattttgac gggcgttttg atgttttgcc gcgcataaaa gccggatgcc aacactttga
    27721 tcataattaa accgattaaa ccgaaagaat aggcaatcag cgcgtgttgc gtcatttgtg
    27781 cgtcaaacag cgtgaattct cggtacataa acagcgtcgc caccagcggg aacgacaata
    27841 ccgccagtcc ggccgccgcc ggcagcgtca gcagcatgca caggcgcaaa ccccagtcga
    27901 gcagggcgga aaactgttcc gtatcttggt ttgccgagtg tttggacaaa gtcggcagca
    27961 aaattgtacc gagtgcagcc cccagcacgc cgccgggcag ctccatcatg cggtcggcgt
    28021 aatacatcca tgaaacgctg cccgattgca gataagacgc gaaaatcgtg ttgataacca
    28081 aagaaatttg cgccacgctc acgcccaaaa tcgcaggcgc catctgtttc atgacgcggt
    28141 tgaccgccgc atctttgaaa ttcagtttgg gcagtttcaa aaagcccagt ttcgccagcc
    28201 acggcagttg gaaaccgagc tgcaaaatac cgccgacaaa aaccgcccac gccagcgcgg
    28261 taacgggcgg atcgaaatac ggcacgaaaa acagtgcgaa tacgataaaa gagatgttta
    28321 aaaacgtggg cgtaaacgcg ggaatgccga acttatggta ggaattgagt atcgagccga
    28381 caaaagaaga caaagaaatc aataatatat aaggaaacgt aatccgcagc aggctgatgg
    28441 aaagttggaa cttgtccgcg tctttggtaa agccgggcgc ggaaacataa atcacccaag
    28501 gcgcggcaag tatgcccagc gcggtaacga cgatcagcac aaacgacagc attcccgcaa
    28561 cgtggcggat aaaagcctcc gtcgcctctt tagaacgcgt ttccttatat tccgccaaaa
    28621 tcggcacaaa cgcttgggca aacgccccct ccgcaaacac gcggcgaagc aggttgggca
    28681 gtttgaacgc gacaaaaaac gcatccgtcg ccatacccgc gccgaatgcc cgcgcaatga
    28741 ccgtatcgcg cacaaatccc aaaacgcgcg acaccatcgt caggctgccg acttttgcca
    28801 aagctccaag catattcatc gtgtttcctt ttcggttgaa accccgccct ttagggcggc
    28861 aggatcagac
//
LOCUS       DAWTHD010000014        26182 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWTHD010000014 DAWTHD010000000
VERSION     DAWTHD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26182
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.014"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            113..2140
                     /locus_tag="WM704_000494"
     CDS             113..2140
                     /locus_tag="WM704_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFA9339985.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AADPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2230..3783)
                     /locus_tag="WM704_000495"
     CDS             complement(2230..3783)
                     /locus_tag="WM704_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFA9339986.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIHKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFEGNK"
     gene            complement(4034..5227)
                     /locus_tag="WM704_000496"
     CDS             complement(4034..5227)
                     /locus_tag="WM704_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFA9339987.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGEAFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLNSRSAAADFPLGGILAQTGSGGSMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5224..6207)
                     /locus_tag="WM704_000497"
     CDS             complement(5224..6207)
                     /locus_tag="WM704_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HFA9339988.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6218..7654)
                     /locus_tag="WM704_000498"
     CDS             complement(6218..7654)
                     /locus_tag="WM704_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFA9339989.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGILAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7784..8949)
                     /locus_tag="WM704_000499"
                     /pseudo
     CDS             complement(7784..8949)
                     /locus_tag="WM704_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9390..9602
                     /locus_tag="WM704_000500"
     CDS             9390..9602
                     /locus_tag="WM704_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFA9339990.1"
                     /translation="METLLLDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9581..9742)
                     /locus_tag="WM704_000501"
     CDS             complement(9581..9742)
                     /locus_tag="WM704_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9339991.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9802..11329
                     /locus_tag="WM704_000502"
                     /pseudo
     CDS             9802..11329
                     /locus_tag="WM704_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11411..12508
                     /locus_tag="WM704_000503"
     CDS             11411..12508
                     /locus_tag="WM704_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFA9339992.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12622..13736
                     /locus_tag="WM704_000504"
                     /pseudo
     CDS             12622..13736
                     /locus_tag="WM704_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            13861..14316
                     /locus_tag="WM704_000505"
     CDS             13861..14316
                     /locus_tag="WM704_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HFA9339993.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLETDSAGTSG
                     WHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFDYIIAMDGKNLSELEKTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAGCRALLEKISK"
     gene            14393..14869
                     /locus_tag="WM704_000506"
     CDS             14393..14869
                     /locus_tag="WM704_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFA9339994.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14857..15783
                     /locus_tag="WM704_000507"
     CDS             14857..15783
                     /locus_tag="WM704_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFA9339995.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15848..16405
                     /locus_tag="WM704_000508"
     CDS             15848..16405
                     /locus_tag="WM704_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9339996.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGLYQNVPVQPVKIRRVVVGQ"
     gene            16492..18549
                     /locus_tag="WM704_000509"
     CDS             16492..18549
                     /locus_tag="WM704_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFA9339997.1"
                     /translation="MDKFKPRILKCCQPNPTQPNPSPDLSDTAGANTEAIYTSDGITA
                     NSTQLEQLKKLFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNL
                     PPETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYNTGS
                     DGFVYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYIAREL
                     LKDDGVIFISIDDNEAAQLKLLCDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWI
                     FVFAKSCQFIAATKGKERRYYETDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTG
                     KKYPANPNATWRVTKDTFQDYYNKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDF
                     GKVAVSSRLPENVGTLADAVAEYLAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPK
                     PSQLIKFLVSISSKSNDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAE
                     KSEARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFS
                     PEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLLNK
                     GFTSADLLAFIKKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYTNKKEIELNVI
                     IRV"
     gene            18639..21578
                     /locus_tag="WM704_000510"
     CDS             18639..21578
                     /locus_tag="WM704_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFA9339998.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(21672..22631)
                     /gene="ttcA"
                     /locus_tag="WM704_000511"
     CDS             complement(21672..22631)
                     /gene="ttcA"
                     /locus_tag="WM704_000511"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFA9339999.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(22723..23586)
                     /locus_tag="WM704_000512"
     CDS             complement(22723..23586)
                     /locus_tag="WM704_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFA9340000.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQAGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAK"
     gene            complement(23621..23983)
                     /locus_tag="WM704_000513"
     CDS             complement(23621..23983)
                     /locus_tag="WM704_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFA9340001.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            24058..24537
                     /gene="ybaK"
                     /locus_tag="WM704_000514"
     CDS             24058..24537
                     /gene="ybaK"
                     /locus_tag="WM704_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFA9340002.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            24559..25251
                     /locus_tag="WM704_000515"
     CDS             24559..25251
                     /locus_tag="WM704_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFA9340003.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIIQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            25255..26130
                     /locus_tag="WM704_000516"
     CDS             25255..26130
                     /locus_tag="WM704_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9340004.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
BASE COUNT         6409 a         6504 c         7212 g         6057 t
ORIGIN      
        1 tttcggccgg tttgtgcatt tcgtcatccg gaagccggta ttttgccggc aggttttgac
       61 tactttgccc gatgaccggg tttgtctgtt tttaacaaag gagtgagaag gtttgtccct
      121 gtctgaattt atagaacgcc gaacgtcatt taatccgatg gttattttga cgactttgtt
      181 ttttgtgtgt gttttggtgg tattggtttt aaccgtgccg gatcaggtgc agatgtggct
      241 cgaccgggca aaagaagtca tttttaccga gttcagctgg ttttatgttt taacgttttc
      301 catttttctg ggttttctgc tgatactctc ggtcagcggt ttgggaaaca tcaggctagg
      361 acgggatgaa gatgtgccgg aattcggctt cctgtcgtgg ctggcgatgc tgtttgcggc
      421 cgggatgggc gtgggcctga tgtttttcgg cgtggcagag ccgttgatgc attatttttc
      481 ggacattacg gtcggcgcgc cggaacacag gcagcagcag gcattgctgc acacggtgtt
      541 ccattggggc gttcacgcct ggtcggtgta cggtacgatt gcattggctt tggcttattt
      601 cggtttccgc tacaaactgc cgcttgccct gcgttcttgt ttttaccccc tgttgaaaga
      661 aaaaatttcc ggaaggttcg gcgatgccat tgatattatg gcgttgcttg ctactttttt
      721 cggcatcatc accacattgg ggttcggggc ttcgcaactg ggcgccggat tgcaggaaat
      781 gggctggatt gccgaaaaca gcttcggcgt gcaggtcttg attatcgccg ccgtaatgtc
      841 cctcgccgtc gtttcggcaa tatccggcgt ggggaagggc gtgaaggtgt tgagcgagtt
      901 gaacctgggc cttgcgtttt tgctgctgtt ttttgttttg gcggcggacc ccactgttta
      961 cctgttgtcg gcattcggcg acaacatagg gaactacctc ggaaatctgg tgcgcctcag
     1021 tttgaaaact tatgcgtacg aacgggaaca caagccgtgg tttgaatctt ggacggtgct
     1081 ttattgggcg tggtggtgtt cttgggcgcc gtttgtgggt ttgtttatcg cgcgcatttc
     1141 aaaggggcgc accatccgcg agtttgtctt cggggttttg ctcatccccg gcctgttcgg
     1201 cgttttgtgg tttaccgtct tcggcaatac ggcgatttgg ctgaatgacg gggttgcggg
     1261 gggaatgctc gaaaagatga cctcctctcc ggaaacgctg ctttttaaat tctttaatta
     1321 cctccccctg cccgaactga cgagcatcgt cagcctgctg gtcatttccc tgttttttgt
     1381 aacttctgcc gactccggga tttatgtcct gaacaatatt acctctcggg acaaaggctt
     1441 gagcgcgcca cggtggcagg cggttatgtg gggcgtgctg atgtctgccg ttgccgtttt
     1501 gctgatgcgc tcgggcggac tcggcaacct gcagtctatg accctgattg tttccctgcc
     1561 gtttgccctg ctgatgctga taatgtgttt cagcctgtgg aaaggcttga gtgcggataa
     1621 gaaatatttt gagaccaggg tcaaccctac cagtgtattt tggacgggcg gcaagtggaa
     1681 agaacggctg gtgcggataa tgagccagac gcaggagcag gatattttaa aattcctcaa
     1741 acataccgca tcgcccgcta tgcacgagtt gcaacgggag ctttcggaag aatacggctt
     1801 gagcgtccgg gtcgataaga tgtttcatca ggacgagccc gcaatcgagt tcgtcattcg
     1861 gaaagagacg atgcgcgatt ttatgtacgg gattaagtct gtcgggcagg atgtatccga
     1921 ccagttgatt aacgacggca agctgccgca tatccggcac cagacaactt acaaacccta
     1981 cgcttatttt ttcgacgggc gcgtcgggta cgatgtgcag tatatgaaca aggacgagct
     2041 gattgccgac attttgaaaa actacgaacg ttatttgatg ttgttggatg atgtcggtca
     2101 ggaactgatg gcgcacgagc aggtggaatt ggcagagtaa atatcctcgc tccggcgttt
     2161 gccaaaaaaa tgcggtgaat cagattcacc gcattttttt gccggacggg gcttcggacg
     2221 gcacggcgtt catttgtttc cttcaaactg ctccttcagc acccgtttca atactttgcc
     2281 cgtagcgttg cgcggcagcc cgtctttaaa gtggatctgt ttggggattt tgaaatttgc
     2341 cagcacggta cgcaggtggc ggcggatttc gtcctcgccc aaatccatac cttccttcaa
     2401 ttggacgaag gcgacgattt cctcgtcggc ataacggtct ttcacgccga tgacggcggc
     2461 ggcttcgacg gcatcgagtt tgtggatttc ttcttcgatc tcgcgcggat agacgttttg
     2521 acctttggaa ataatcaaat cttttttgcg gtcgacgata aagataaagc cgtcctcgtc
     2581 tatggtaacg aaatcgcccg ttttcaacca gccgttgacg atggtttcat cggtggcggc
     2641 aggcatattg aggtagcccc gcatcaccga accgcccctg acgatcagtt cgcccacttc
     2701 gccgcgcggc acttcgacca attcttcatc gacggctttg gcttccaaac cgggcagggg
     2761 gatgccgacg ctgcgggctt tttgccgttc gggcgtattg acggcgacga cgggcgaggc
     2821 ttcgctcagt ccgtagcctt ccagcaattt ggcgcggggg aacttggctt taaaatcgag
     2881 gatggtttgt tccgccaaag gcgcgccgcc gctgataaac aggcggatgc ggttgaacca
     2941 tctgaaatac caagggattt ttgccttgct catcgcggtg taaatcgcgg gtacgcccaa
     3001 aaacacggtt gcgcgtttga gcagggcctg tttcaaaacg ttggaaaagg ggaaaacgga
     3061 tttgaccaaa ataatcgaac acgccatata aatcggcagc agcaccatag ccgtcagcgt
     3121 gaagctgtgg aacatcggca ggaaaacgat aaagcggtcg cgtttggaaa ttttaaagat
     3181 gcgttcgatg ccgttcaggt tggcgaacag gttggcgtaa ctgattagcg cgcctttggg
     3241 atgccccgtc gtgccggagg tgtagatgat gtgtgccaaa tcatttatcc ggggttggcg
     3301 gcccaagtcg ggtttttcgg ggaagcggcg cacgttttca aaaaaggcat cgccttccgc
     3361 cgtttcgccg gccggccggc ttttgtccgt ccaaatgatt ttttcgacgg gcgtttgcgc
     3421 cttcaggccc gccaattctt ttgacaggcc ggccgaggcg aacaggaagc gcgccttgca
     3481 gtcgttcagg atatacgcgt attcgctgtt tttcaaaaat gtgttcatcg gtacggcgac
     3541 cgcgccgatg gcggagacgg cgaaataggc ggtaataaat tctgtggaat tggaaaccgc
     3601 cagcgcgacc gtgtcgccga acttcacgcc gatattttgc agatacgccg cgacggcttc
     3661 ggcttcctgc ttgagcgcgc ggtaggcggt tttttccttg ccgtcgaaca ctgccgtgcc
     3721 gttgccgttt ttgcggcagg cggcggcgag catttcgtag aaattggcat aagtccggtt
     3781 catgaaactt cctttatggt tgcggacggc gaacgccctc atttgaaaac aatatgccgt
     3841 ctgaaaacgg aaggcggcag tttatcatct ttgccttcag acggcatatc aaacgccgcc
     3901 tttgcgccgc atgccggagg ttcgcccccg tccttcctat gtctgacctg aatgccgtct
     3961 gaatcaatat gaagatatac gtttcagacg gcatccccct gttgtggaaa taccgtccgc
     4021 cccccctgtc cgattattgc ctgattgcgg cggcgcggac gagttcttcg aggctgtcgg
     4081 caagcatttc cgccgcgcgc cggtagcctt gggcggagaa gtgtacgccg tctttggcgg
     4141 cccatccttg gttgagccag tttttcatgc tgcatatgcc gcccattgcg ttttgccaag
     4201 accaaaacat cgtctgcccc tgacgggcga cgcgccgctg catctgttgg acttcggtca
     4261 ggaggacggg gcgcgtgccg catacgccga gcgtgttttt cagggattcg ggcgcgccga
     4321 tgatgaggat gccggcggcg ggcaggctgt cgcggatttg gcggacggta tccagccatt
     4381 tttgttcggt atcggcaatg tcgatgttgt tgttgaaggc ttcgttggtg ccgtaggaaa
     4441 ggataaccaa atcggcgccg gtttgggcaa ggtcgttcat acggtcggca cgccatttcg
     4501 accactgggt caattgtgcg ccgttgatgc ccatcgcgga aacggtaatg ccgccggcgg
     4561 gattttcgat gttgatgaag ccgatgtccc acggcatttc ggtctgtatg gccaggggca
     4621 gtgccgcgcc cgtatccagt acctgccagc cgccgccgtt ggcggagacg gtgttgccgt
     4681 tgacggtcag ggtttgttcg gcgagcagcg gtttggcaaa cagggaaacg cgctgtttgc
     4741 cggttttgcc gtcagacgcg gtcagggtca tgctgccgcc gctgccggtt tgggcgagga
     4801 tgccgccgag ggggaagtct gctgcggcac ttcggctgtt gaggctttgc cagttgccgt
     4861 tatagcggac ggccgccatg cgctgccctt tgacgttggc ggggtaaacc cagcctatgc
     4921 cgccgtcgcc ccatgttttt tgcaggcgtt tgcgcagggc gtcggtaaag aagtcgccgg
     4981 cggtatgcga gtcgccgatt tgcaggatgc ggaaggcctc gccgctgcct tgtgcgacgg
     5041 atcggagttt tttcatccaa ggcgaggcgg cggcgttgcc gtagtcggtc agcagcccgt
     5101 ttgtatcggt gtagggggcg gacggggaaa cggtaacggt gtcggggctg acggaggcga
     5161 cgggcagggc ttctgcctgc gtggcggcga acagggcgga aaatgcgatg aagtgtttgg
     5221 ggttcatggc tgtgtacttg atggttgcgt actcggttca aaaacgattt tttccattat
     5281 tttttccgcc agcagttttt gtccttcggc ggtaaagtgt atgccgtcct tactgcggta
     5341 gcggacgggt ttgccgttga cgttgacgga atcggtgtag cggcctttcc cgccgctcag
     5401 tgtttgcgcg gtgggaatca ggatgatttt gcctttcaag tgttccgaaa gcagtttgtc
     5461 gaggtagcgc atctgaccgt cgagcttgac ttttttcatg taggggatgc cgagccagac
     5521 gacttggacg cggtgcgtgt gtgcggcttc aaggatgcgg tcgacgcgtt tcaggtattc
     5581 ttgcgcccat tcgtcggaag cgaatttgag gtagcgtttg ccgacgggga aatcccacgg
     5641 gtcgttcggg ccgaggaaga cggcgagtac gctgatttcg ggatgttttt tcaaggtttc
     5701 ttcaatcgtt ttcggccagt cgaagaatga gggataggaa agccccgtgc tttgtttgct
     5761 gaggttggcg gattcgatgc cgtattgctg tttcaggctt ttttgcacga agggcgcaac
     5821 gccctgcatc agcgaatctc cggcgaaaaa gactttgtcg ccgctgcgga cggcggcggc
     5881 ttcggtgcct tgtttccatt ctgttccgcc ggccggcggg gcggcttcgg atgcggcggc
     5941 ttcaggcggc atatctgccg aaccgccgtc ttgagccgtc ggcggcgttt cgccggacag
     6001 gaaggttttg atgccgtctg aaagggcgta ggcgttttct tgcaacgccg ctccgctccg
     6061 ccaccatccg taggcggcaa gcggttcgag cgggctgttg cggtggtagg tctgctgcca
     6121 gtaggcgttg atggggtttt ggctgaacca cacggcaatc agggcagaca tcagtatgga
     6181 ggcgaaaagg gaaagaaagt ttttcatgtc caaaccctta aaaattggca taaataaagc
     6241 cgggtatccc cgaaggggcg aggacgataa tcagcagcag gatgatggaa atctggataa
     6301 accacagcca catcgggatt ttttccaaac ctttgaccgc gccgtcgaaa gcgcgttgca
     6361 ggtaagggta gagcagcatc aaggatgcaa acgaggcaag cagcagcata tccgcccgtt
     6421 gcggcgcgtt ccagccgccg gcattggcaa agagggcgct gaaaactgcg cctgcatcgt
     6481 cggggtttgc ggtattgaag acgacaaagc taaggcagac gaaatggaag gtaacaaacc
     6541 acgagagcgg cgcaaggtat ttcagacggc atagcgcgtc gcgtccgaaa taccggtcgc
     6601 ccgtgttgag cagcgccagt gccgtgccgt gcagcgcgcc ccaaatgagg aagttccagc
     6661 cgtagccgtg ccagatgcct gagagtacca ttgccgccat caggttgagc tgtgtccgca
     6721 aaaagccttt tttgctgccg cccaagggga tgtagatgta gtcgcgtatc caggtggaaa
     6781 ggctgatgtg ccatttgtcc caaaatgcgc ggatattcgc ggcacgaagc ggtgcggaga
     6841 agtttttggg cagccggaag cccagcagca tcgccatacc gataaccaaa tcggaatatc
     6901 cggaaaagtc taaaaagagt tggaaggtat agccgtacac gcctgccaac acgccccagc
     6961 cgtcgaattg ggcgggattt tcaaatacgg gcgacaccca gttttccgcc agtatccccg
     7021 ccagccacca ttttttggca atgcccagta aaatcaggga aacggcgagg gcggggcgga
     7081 cgggcgaacg cggtcggcgg gtgcggattt gcgccagtgc acccgcctgt tcgccgtccg
     7141 tgcttttgaa tgcggcggcg cggataatcg ggccggaggt aacggtgggg aaaaaactca
     7201 ggtgcagcag cagctcgtgc cacccaaaac gcgcggcgtg cggggcgcgg aagcagtaaa
     7261 ccagataggc gacagactgg aaggtataat aggaaagccc cagcggcatc aggatgtcga
     7321 ttgcgccgcc ttttccggca tattgtgcaa tcagcggacg gaaaaaatcg aaatatttga
     7381 aaaagcccaa aacggtaatc gaggcggcaa tgccgcaccc cagccaaaag cggcgcgtac
     7441 tttcgcgatc ggaacggagc agttcgccca aaaggtacac gcaggaggaa taaaggacga
     7501 tgattgccgc aaatacgggg ctaatatggt agagccagcc cataccggca gccaaaagca
     7561 gcaggttttg gacggacggg tatttcgcaa agccccagta aatcggcagg aagacgataa
     7621 aaaacagtgc gaactcgaca gacagcagcg gcataaacca tccttgggac aaaaaaatag
     7681 gcgttaaaac acggcaaagc ccgttatttt aagcaggggc ggatattcct tcaatcaaaa
     7741 tgccgtctga agggcgggag gggcttcaga cggcatcgct cggtcaggtt cggatatggt
     7801 ggttcaaatt caggcagacc cgcaaacctg ccgccgccgt cagcataccc gcaacgccga
     7861 tgcaggaaat gcccgagtat tgcgtaacct aatgtcccag cagtgcgccg ccgcctatac
     7921 cgacgttgta aagctccgaa taaatcgagt tggcaaggtc tgcggcatcg gaggcgaaat
     7981 ccaaaacttt ggcaaccatc cccaagctga cgaccacaat cgccgtcccc catacgaaca
     8041 ccaacgcgta aatggcggca ggaaaatgcg ccaaaggcag caaaagcccc gaagagaggg
     8101 cgataagcga caccgcgccc gccaaaaatg cgcgcggatg tttggcaaac catttgccga
     8161 acaggtaaga cgcggcaaaa cccgccaggc cgtacagccc cggaaccacc gtaacctgcc
     8221 gcgcggagaa accgccgatt tggatgacga agggctcgat gtagctgtat gcggtaaagt
     8281 gcgccgtaat caccggcacc gttaccgcat acagcagcat cagttttttc cgtttcaaaa
     8341 gcaggggcag gctgcttaaa gaaccggtat tcacgctcgg caggcgcggc aggcttttgg
     8401 caagcactgc cattaccgcc gccgaacaca ggccgatcag cagaaaactc gcctgccagc
     8461 ccaaatattg tccgaccata cgcccgagcg gaatccccgc caccatcgcc atcaccgtcc
     8521 ccgtgctgag cagccccaat gcctgattcc cttttccgac aagggcgatg cgtaccgcca
     8581 gcgaagccgt aatggaccag aacaccgcgt gcgtcagcac aatccccacg cggcttgcga
     8641 gtaggatttc aaaacgccac gaggcaaacg acaggatgtg gctgacagta atacagcgca
     8701 aacaggacca gcagcaggcc gcgccgttcc ataattgcgc gtcagcagca tcagcggcag
     8761 cggcagcgaa gtcagcgcga caatccacgc ataaaccgta atcatcacac ccgtttcggt
     8821 cgccgccatg ccgaagcttc gtccgatgtc gctcaaaagc gcgatgggga tgtattcggc
     8881 cgtgttgaaa ataaatgcgc cgattgccaa ggcaacgatg ctcaaccaag gtttgtgcga
     8941 agcgttcatc tcaattcctt atatgtcgca ccccgccaaa agggggcgca ttatggtgaa
     9001 ttaacaaaaa ccggtacggc gttgccccgc cttagctcaa agggaacgat tccctaaggt
     9061 gctgaagcac caagcgaacc ggttccgtac catttgtact gcctgcggcc cgccgccttg
     9121 tcctgatttt tgttaattca ctatactttt cttgtctgcc ggacggcaaa atccaaatca
     9181 gggggaagca gccgcctccc gttcggagaa acactcggga cgctgctttg aaaatcgttc
     9241 catatgttga aacattgccg cctcccgatt gggcaaggtt gccattatgt cgcaagcatc
     9301 cgtgccgcac cgtttccgcg tttgcccaag ttcacgctcc gacttacaat aacgcccgtc
     9361 agattgcagt accaacgaaa ggaaaaatcg tggaaaccct cctcctcgac atcggcggca
     9421 tgagctgcgg cggctgcgtc aaaagcgtta cccggatatt agaaagcgta aaaggcgtgg
     9481 caagcgtcga agtcagcctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa
     9541 ccgcagcgga agcactaatc gaagccgtag aagacggcgg ctatgatgcg gcgttgaaat
     9601 aaagcggcaa aaatgccgtc tgaagccttt gcgtttcccc gatgccgtct gaagcacgct
     9661 ccgcgccttc gggcggcatt tgatttagtt gaacgggcag gatgaattgg ttaaacttgc
     9721 gggcaatagt gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg
     9781 cgcgctgcaa cggagaacgg tgtgccgggc gtgtccgaca tcaaaaattc atatgatgat
     9841 ttgatgtacg aatccaaagc attttcccaa accgccatca acgccttgga ggcatgcgcc
     9901 cgcatgatgg gtttgaagcc tacggcagcc gcaaaagcca aggtcttgga gctgggctgt
     9961 tcgatgggca gaaacatcat caacgcaggc actttattac ccggatgcgg aatttgtcgg
    10021 tatcgacttg tccggcaggc aggttgcgca gggcaacgcc atcattgaaa aaatgggctt
    10081 ggaaaatgtg cgtctggaag aaaaagatat tttgaccgtc gatgaatcat tcgggaagtt
    10141 cgactatatc atcgtccacg gcatttggtc gcgggtgccc gacgcagtta aagacaaaat
    10201 cttttcgatt tgccggaaca acctgaccga atacggcatt gcctatattt catacaatgt
    10261 ttaccccggt tggaagcggc aggagcagtt gcgcgatatt atgcagtttg ccggcaggga
    10321 tgcgcttgga gaacccttgg aagcaaggac gcggaaaggc ttggatgcga tcaaggcgct
    10381 ggcggaaatt ttggaaaacg acaagggctt gggcggcggc aaacttccgg cgattcaaaa
    10441 aatattgaat cacaatactt attatgtcgc acacgaatat atggaaatat tcaacgaccc
    10501 gatttacgtt aacggtttca ttgaatgggc aaaccgccac cggctggcgt atatcgggga
    10561 taccgatttg catgtgtctt ttgtttcttg gatggcggag catacgcggg agcggattct
    10621 ggcattggca gggggtgatt atattgccaa agaattttac agcgatattt tatccgaccg
    10681 ccaattccac cgttcgcttt tatgccgtga ggaagtcggg gatactgtca ggcgtgatga
    10741 gtcggttgcc gtcgaagtga tagaaagttt gaatttccgc ccggcaagag gggaaacaat
    10801 caactttgat gaaaacgata ccctgctttc gggcatacgc gatgtgatga aaaccggaga
    10861 ggcgtttaaa acggaagatg ttgcggaaaa tcttgcccgc cgatttcccg gtctggaatt
    10921 tgaccgcatg aaaatcaatt cccagctttt attgcaaacc attctcgggc gtttttctgt
    10981 ttcatcagac aatgcgggca aaccattttt tgaagaccat aaaacctatg tgccggcgcg
    11041 ctttacaaac tatgccgccg cctttgtgga acacggtgcg ggagcgtttg tccggcctgc
    11101 caaccgttac aacgaaagca cgccctcatt cggatacggg catttgtaca ttatgcggca
    11161 attgtcgcgg ccgacgagca aacaggcatt gattgaaacg gttgccgaaa acttgaacat
    11221 cgtcagcgcc acgcccgacg gtttgacatt ccatccgcct gccgaagtgt atgtggaaga
    11281 aatattggca gacttggcag acaggcattt tctcgtttcg gcggattgac gggcaagtcc
    11341 cgacggcaat ccgtccgata tattttcaga caggaaagac acacactgcg aaaaacggta
    11401 cacgacacat atgaaccaga catttacctt gcccgatacg cgcccgtatc cccaaaatcc
    11461 gattaaaaac cacctgctgc tcaatgccta ccagttggcg cataattctt cccaggcttc
    11521 gcgcaaactc tcgtccgggc agcttcaaac cgaaatcagg gggatgcttg agcaaaacca
    11581 ctatatcaac ctttccctcg cgctgacgat gtcgcccgat gccggaactt atgccgcgct
    11641 gctttccagt gtgaacgcgg tactcgattg cgagaaagaa ggcgaagtgc agtggttcgc
    11701 cctgccggtc gtgctggtgt caggctgcaa gaaagaacgg gcaatcgaga tgaagctgcc
    11761 gacggaggca ttgtttgcct gcctgcaaaa ctatccgcac ctgcgcgcgt tgacgcaaga
    11821 gacacaatgg ctgccttatc ttgtgcattc ttccgatttg agtgcggtcg cgccggatga
    11881 gtggtggcgt gccaaacaaa ataccgaagc ggcggcgcaa cacttacgcc gtttcgcccc
    11941 gcgccctttg ctgttgcccg aagggcagtc cgtccacgtc gtttacgtgc tgggtttcgg
    12001 cagcggcaaa gttcaggcgg cattggggca gaaccttctt caggcaggct tgcccttaat
    12061 gcaggtatgg caggaaaatc ttgcatcgga aggcattacg ctgtttgcca atccgctttc
    12121 ccccgatacc ccggcgcgcg cgctttcaga cggcagccac acgcgccaac gtatggcgat
    12181 ggatgttttt gcggcaaacg cgatacgcgc cgtccggatg cagggtccgc gcgtcggcgt
    12241 ggtcgctgcg gcaaaggcgg gcggacagat tctattcgga tttaatgcga ccgacggcgc
    12301 gtttgaagtc gtgccgcagg tgttttgctg gcagctttcc tttaccgata acattgccgt
    12361 catccagcag aatttcctcg acctgatggc agaatgccgt gtagaacacg tttacctgct
    12421 gcacaatccc ttgggcgaac aggaaaacat cccgagctat gcggaagcgt tgaaacggga
    12481 agggcgcaat ccgtttttca gcgcatagtg atttgtagta gtgataggct ttctcatttc
    12541 tataataaat caaaataaag agcacaacac tttttcattc tgtgttgtgc cttgagtgaa
    12601 acgaaaggat gaattataaa tatgaagatt gtaattgcac cggattcgtt taaggaaagc
    12661 ttgacagctc aacaggtagc tgaagcaata aaaagaggct tccaacaatc gatagcagat
    12721 gtggaatgtc tcctctgtcc tgtcggtgat gggggagaag gcactgtaga tgctatccga
    12781 cattctcttg acctagaaga aaaatgtctc caagtgacag gaccttttgg acaaaaaaag
    12841 tcatgcgcta ttttcaaaaa gaacaactag ccctattcga agttgctgat ttggttggtc
    12901 ttggaaaaat cccgctagag aaacgaaatc cattacaaat ccaaactcgt ggtattggag
    12961 aattgattcg ccacctcatt agtcaagaaa ttaaagaaat ctatattggc gttggcggta
    13021 cggccggtaa tgatggaggt attgggattg ctgctggttt aggttatcaa ttttatgatg
    13081 aggatggaaa tgccttaccc gcttgcggtc aatccttact aaacccagct tctgtttcaa
    13141 cagaaaatcg ctatgaaatt cctgaagatg ttcacattcg tattttagca gatgttgtga
    13201 gtcccttatg tggacaccaa ggtgcgacct atacgtttgg caagcaaaaa gggctggatt
    13261 ctactatgtt tgaggccgta gatcaggcaa tacaagattt ttatgaaaaa gtcccccctg
    13321 caacattaaa acttaaagga gcaggagctg gtggaggcat cgctggtggt ttgtgtgcct
    13381 ttgctcaggc aagtatcgta tctggaattg acacctgcct ggacttgatt gactttgata
    13441 agaaagtgtc agatgttgac ttggttatcg ttggtgaagg aagactagat cgtcaaagtt
    13501 tagcagggaa agcgcctatt ggtgtagcaa aaagaacccc tgtcggagtt cctgttgtcg
    13561 ctatttgcgg cagtttcgct gaagatttgc cttccctacc atttgaaaat atccaagccg
    13621 ccttttctat tttggagaaa agtgaacctt tagaagatag tttgaaaaat gccagtcttt
    13681 atttggagca tacggcttct aatattggac atttattaaa tatgcctaag atttagccaa
    13741 gccatttctt ccagatggat gttttggcag gttcttcctt atgcggttac gattaaccgg
    13801 ccgatgccgt ctgaaagggt ttccgcttca gacggcatcg cgttggaaca gggatgggat
    13861 atgaaaaaac cgaaaatcct ttttgtctgc ctcggcaaca tctgccgttc gccgatggcg
    13921 gaatacattt tgcgccgccg tgccgccgaa gcgggcattc ccctcgaaac ggacagcgca
    13981 gggacatcgg gctggcacga cggcgaggat atgcaccgtg aaacggcaaa gatattgaaa
    14041 aaatacggca ttgacgcttc aggctttacc agccgcaaaa tccgccaaag cgatgcgacg
    14101 gcgtttgact acatcatcgc tatggacggc aagaatttgt ccgaattgga aaaaactttc
    14161 ggcaggcggc cggaaaaaat attcaagctg accgacctga tacccgaaag cggttacgac
    14221 catgtccccg atccgtggta catgggtgat tttgaagaaa catacaggct tgcggatgca
    14281 ggctgtcggg cattattgga aaagatttcc aaataaagca attgaataca aacacaaata
    14341 taaaaccgcc tcctgtcgta tcggtcgttc ggacggcata aacagggaat ttatgaaaac
    14401 aaatttcaaa cagaaaatta tcgaacaggc acgcagagag gacttgcagg taaccgcttt
    14461 gcgcgagcag gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaatgt
    14521 tttgtcacag atgcagcagc agagcgaagg cgtgcttgca ccgcctaccg cctaccgtgc
    14581 ccttgatttt tgggcggatc agggcgtttt acacaaagtg gcggcggtca acggctatat
    14641 tttgtgcagc cacgcgcagc acgagtgcaa cgaccattgc cacgaccacg aagaagccga
    14701 agcgcaccac agcgcgttta ttttggtctg caccgaatgc ggcgcggcgg acgagcaaac
    14761 cctcagccac gagtgggcgg cactgcgtgc aggcgttgcc gaaagcggtt ttgcgctgaa
    14821 agaagaacac gttgttttaa ctggaatctg taaaaaatgt cagaagtgaa aaaaaccaaa
    14881 gtccacctga tttcaggttt tctgggaaca ggcaaaacca ccgcgctcaa aagcctgatg
    14941 gcgcaaaaag acccgaacga aaaatgggtc atcatcgtca acgagttcgg cgaaatcggc
    15001 attgacggcg cggtattgag cgacaacggc atccctgtgg cagaaatcgc cggcggctgt
    15061 ttatgctgca ccgccggccc gcaaatgggc gtaaccgtgc agaaaatgct gcgcgacgcc
    15121 aaacccgacc gcctgatgat tgaggcaagc ggactggcgc acgccgccag cgtcatcgac
    15181 gaattgaaag ccaaacctct ggacagtctt ttggaaatcg gcgccgtctt taccgtcgtc
    15241 gatccgcgcc agttcatcaa tcccgattac gcgcaacagg cgttgtataa agaccaaatc
    15301 ggcatttgcg acgtattggc cgccggcaaa accgatttat gcacccccga acagcttgcc
    15361 gaatttcatg acaaagcggc aaaactgttc ccacccaaag ccaaagtggt cgaagtccaa
    15421 aacgcacaac tcgatatcca atggctcgat attcccgtag ttgaaaaatc acgctaccgc
    15481 ctcaaagccc tgccggacaa caccatgggc ttccagtcgc aaggtttcac attccccgcc
    15541 ggacgcgatt tcgacggcga aaaattgacc aacttcttca atgatttgcc caaaatgacc
    15601 gaaggactcg tccgcgccaa aggcgtgttc caagtattgg gaacgtgggt gtggctcaac
    15661 tgggtggacg ggcagtgggg cgcgaaccaa gtgtcttggc ggcgcgattc gcgtttcgag
    15721 ctgattgcca aatcgtttga cgcggattta atcgaacaaa aacttaaaga cgcattggaa
    15781 tagcgttcac gcgctttcag acggcatatg aaatgaaaat gccgtctgaa ggctttagga
    15841 ggacgggatg aaacccaaat tcaaaaccgt tttaaccgcg ctgcttttgg cggtttccct
    15901 gccgtctatg gcggcaaccc gcgtcctgat ggaaaccgat atgggcaata tccgtttggt
    15961 tttggacgaa tccaaagcct ccaaaaccgt tgccaatttc gtgcgctatg cccgaaaagg
    16021 cttttacgac aacacgattt tccaccgcgt catcggcggc ttcgtcatcc aaggcggcgg
    16081 attgaccgag gacttggtgc aaaaggcaac cgataaggcc gttgccaacg aatccggcaa
    16141 cggcttgaaa aacaccgtcg gcaccatcgc aatggcgcgg acggcagacc ccgattccgc
    16201 cgccgcccaa ttctttatca atctggcgga caacggttcg ctcgactaca aaaacggaca
    16261 atacggctac accgttttcg gcagggtaga aagcggaatg gacaccgttt ccaaaatcgc
    16321 ccgcgtcaaa accgccacgc gcggccttta tcaaaacgta cccgtacagc ccgtcaaaat
    16381 ccgtcgcgtt gttgtcgggc agtaacacgc agacagacgt tcagacggcg tcgcccgttt
    16441 cccaaaaaac gccgtttaaa gtaaaaaaat attttaaaac agacagttga tattgacaaa
    16501 ttcaaaccga ggattttaaa atgctgccaa cccaacccaa cccaacccaa cccaagccct
    16561 gatctaagcg acacagccgg ggcgaacact gaggcaatct acacttcaga cggcattacc
    16621 gccaactcta cccaactcga acagctcaaa aaactgtttc ccgcctgttt tgacgcagac
    16681 ggaaatttcc ttatcgacag attacaagcc gaaattgcgc cacagaccga catcggacgc
    16741 gaattttacg aaatgaactg gctgggcaaa tcatatgccc gcctgcttcg caacctgccg
    16801 cccgaaaccc tgataagtga agacaaaacg cacaacgcca agcccgaaaa tgcaggcagc
    16861 caaaatctgc tgattcgcgg cgataatctg gaagtgttga aacacttaaa aaacgcctac
    16921 gcaaacagcg tgaagatgat ttacatcgac ccgccctaca acaccggatc ggacggcttt
    16981 gtctatcagg acgaccgcaa attcacaccc gccgaacttg ccctcctggc gaatattgac
    17041 gaagacgaag ccgcgcggat tttagatttc accgacaaag gctcaaactc gcacagcgcg
    17101 tggctgacct ttatgtatcc gcgcctgtat atcgcccgcg aactgttaaa ggacgacggc
    17161 gtgattttta tctcgattga cgataacgaa gcggcgcagt tgaaattgtt gtgtgatgaa
    17221 gtgtttgggg aagggaattt tgttgcacaa ttgccttggc gaaaaagaac agctaaatca
    17281 gatgtgcctt ttggtatttc gcaggattat gaatggatat ttgtattcgc aaaatcttgc
    17341 caatttattg cagcaactaa aggcaaggaa cgacgctatt atgagactga tgatttcccc
    17401 gatcgtcctt ggcgtaccca cgacttaacg aaacaaacaa ctgcggcgga aaggccaaat
    17461 agttttttca caatggttga tcccaagaca ggaaaaaaat atccagcaaa tccaaatgca
    17521 acttggcgtg taaccaaaga tacatttcaa gattattata ataaaggaaa aatcgttttc
    17581 cctgatgatt atgatttctt aaatatcagc aatccagtta tgcgttactt taaagatgac
    17641 gacatgaaaa aagctggcga ggattttggc aaagtagctg taagcagtag attacctgaa
    17701 aatgttggta cattagccga tgcagtagcc gaatatttgg ctatttttag taggacgcta
    17761 cctgaaaata tcggaatgac taaagaaggc acaaaagaaa tcacagattt atttggtagc
    17821 aaaatattta ccttccccaa gcctagtcaa ttgattaaat ttttagtttc aataagttca
    17881 aagagtaatg acctaatcct agacttcttc gcaggcagcg gcacaaccgc ccacgccgtg
    17941 atgcagctta acgccgaagg acaaaacggt aaccgccgct atatctgtgt acagcttccc
    18001 gaaaaaaccg ctgaaaaatc cgaagcccgt aaagcaggct acccgaccat cttcgacatc
    18061 accaaagccc gcatagaaaa agccgccgcc aaaatccgcg tcgaacatcc cgattacacg
    18121 ggcgattcgg gcttcaaaat ctttcaaacg gcagacaatt tcaggcagca tccagacaag
    18181 gatttttcgc ccgaacaacc ggatttgccg cttaacgatg aattaagcga agaacagctg
    18241 caaacgcttc tgaccacctg gacgctgtat gacggggcgg cactgaccac gccggttgag
    18301 cctgtgcggt tgggcagtta tacggcgtat ctgtgcgaaa aacggctgta tctgcttaac
    18361 aagggtttta cttccgccga tttgctggcg tttatcaaga agctggacga cgatgcggat
    18421 ttcaatccca accgtgtgat tgtattcggc agcaatatgg caagcgccat gcagcacgaa
    18481 ctcgaccagg cggttcgcgg ttataccaat aaaaaagaga ttgagttgaa tgtgattatc
    18541 agggtgtaac cggaatccga aagtgcaggc tgcttttcgg cttggattcc ccttgcgggg
    18601 gaaggacgat acaacaatga cttcatggag aaacattcat gagcggtttt aattacgaga
    18661 aaaaccagcc gcaccaaatg cgggcggttt cggcggtttt gggcgtgttt gacggggcaa
    18721 cgcccaaata tcggacggca gacgaaaatc ccgaactttt gtttgctgca aaacaatacg
    18781 caaacaatat cctgaaagtg caaagccaaa acggtataga cggccgattc cccgaccgtt
    18841 cggacgacca aaatatcctt gatatttcca tggaaacggg cacgggcaaa acctatacct
    18901 acacacaaac catgttcgag ctgcaccgtt ggctgggcgt gttcaaattt atcgtggtcg
    18961 tgccgacttt gtccattaag gcgggaacac agcagttttt gcaaagcaag gctttggcag
    19021 agcattttga acaggatttc ggcggcgatt atgaaggcgt acgcctgaaa acctatgtgg
    19081 tggaaagcgc gaaaaagaat aagggcaaaa agtccaatgc gcccataacg attgagcaat
    19141 ttgtcaaagc ggaaaacaaa aaggaaattc atgtgctgct gattaacgcg ggcatggtta
    19201 attcgtcgtc catgaacgat acgggcgaca aggcattgaa ggatttgttt gacaatcccg
    19261 ttgatgcatt ggctgccgtg cgcccgttta tgattgtgga cgaaccgcat aaattcccga
    19321 cccgagatag cgcgaaaacg tggggcaata tcaaacgctt aaaaccgcaa tatattttgc
    19381 gctacggtgc aacatttaac gatgaatatt acaacttgct ttaccgtttg acggcagtag
    19441 acgcgtttaa cgacgggctg gtcaaaggcg tgcgcgtgtt tcaggaagaa atgcagggcg
    19501 gcatggatgc ggcggtaaaa ctggtgtcgt cggacggcaa agaagcgaaa tttgaattaa
    19561 acgaaaagga caaaaagcag acgttcaaac tggcaaaagg cgaagatttg gcgcaaatcc
    19621 atccggctat ttcggatttg aaaatcgaca aaatgaataa aaccgtggtg gtgttaagca
    19681 acggcttgga gttgaaaacg ggtgccgtca tcaaccctta ttcctattcg caaacggtgc
    19741 aggatgcgat gatgcagcgg gcggttgccg aacatttcaa gctggaacgc gcgcttttgg
    19801 cagaacgcgc gccacagccc aaaatcaagc cgctgacgct gttttttatt gacgatatcg
    19861 cgggctaccg cagcggcaac gagctttcag gcagcctgaa agataaattt gaaagctgga
    19921 ttcgcgcgga agcggcacgc cgtctgaaaa cggaaagcga cccgttttac cgcgattacc
    19981 tgcaaaagac gttggacgat gtatccgcct gccacggcgg ctatttttcc aaagacaata
    20041 cagacagcga cgatagaatc gagcaggaaa tcaatgaaat cctgcacgat aaggaaaaac
    20101 tgctgtcttt ggacaacccg cgccgcttta ttttttccaa atggacgctg cgcgaaggct
    20161 gggacaatcc caacgttttc cagatttgca aactgcgttc cagcggcagc acgacttcca
    20221 agctgcaaga agtcggacgc ggcctgcgcc tgccggtaaa cgagctgatg gcgcgggtgc
    20281 gcgatgtacc gtacaaactg aattattttg tcgatagcag cgaaaaagac tttgtgaagc
    20341 agcttgtcgg cgaaatcaac gacaattctt ttcaggaaga aatctccaaa aagtttaccg
    20401 aagagctgaa acaaaaaata ttgcaaaaat accccgatat caaaccgctg gtattggtaa
    20461 accaactgtt ttcagacggc atcattgacg acaatgaaaa ctttgccgaa gacggctatg
    20521 acaaattaaa agccgcctat cccgaagcct tccccaaagg tttggacaaa ggcaaagtca
    20581 gcaacgccaa agacgagggc aaagacacca tcatcatgcg cgaaggcaaa tatgaagaac
    20641 tcaaagcctt gtgggagctg attcaccata aagccgtttt gcagtacaaa atcaaagatg
    20701 aagccgaatt tgtcgattta tttaccgcct atttgcgtga aaacgccgcc aaattcccgc
    20761 aggcaggcat atgcacggcg gtaaacgaag cttatatcaa caacgggctt atgctttccc
    20821 gccgcataga cagtattgaa gatgaagatt ttatccgttt caacacaatg acttaccgtg
    20881 agtttctgga aaaactggca caaacggcaa aaatccagat gcagactttg catcaggcgt
    20941 tttaccgcgt ccgcgacgaa ctgaacattg gcgatttttt gaatatgcag accatcgccc
    21001 aaatcaaaaa cggcttcaac cggtttttgc ttcatcattc cttccataaa ttcgaactgg
    21061 attaccggct tgtcggcagc aaaatccatc cgaccaaatt taccaataaa gacggcaaac
    21121 cgcgcgcggt gaaaaaagca gatttgggca gatttgaaga tacggagcac cggcctgccg
    21181 ccggctatct cttcggcgag attttctacg attcggatat agaacatgaa aatgtcgcca
    21241 acaaccaaat tgaaggcgta atcgtattta ccaaaatacc gagaaactcc atcaaaatcc
    21301 ctgttgccgg cggcggcacg tattcgcccg actttgccta tatcgtgaaa accaaaagcg
    21361 gcgagattct gaactttgtg attgaagcca aagggactga cggggcggaa gatttgcgaa
    21421 aaagcgaaga gcggaaaatc aaacatgccg aaaagctgtt tgccgagatt tccaaagaaa
    21481 tcaaggtggt gttcaaaacg cagtttgacg gcgagaggat agccgaactg atcgggcaaa
    21541 atatgccagc aggcgggcat tctgaaaacg gacactgaga aacaaatgtt tggaagccgt
    21601 ctgcttcggg attttaaaat gccgtctgaa acgccaaacg ggcttcagac ggcatttttg
    21661 acggtggagg tttacgcgcc gcaggttttc ggcttgttcg ccagaatgtt gatgactttg
    21721 cgttcggctt tttgcggctc gattttgatt tcgctctcat cttcttcgct gccgtctgaa
    21781 aagcgttcgg gcattttttc gctgtcaaaa gccaaatcgc cgccgtgttt cagattttga
    21841 ccgcgttcca atccggcaaa gtcaaagagt tcggtatcgg caaggtggga agggacgacg
    21901 ttttgcaggg cggagaacat cgattcgata cggccgggga agcgtttgtc ccaatcgcgc
    21961 agcatatcgc cgatgacttg gcgttgtagg ttgggctgcg agccgcagag gttgcacggg
    22021 ataatcggga actgtttcaa ttcggcgtat ttgattaagt ctttttcttt cacatacgcc
    22081 agcgggcgga tgacgatgtg ttcgccgttg tcgctcacca gcttgggcgg catggctttg
    22141 agtttgccgc cgtaaaacat atttaaaaac atggtggcga ggatgtcgtc gcggtggtgt
    22201 cccaaggcga ttttggtgca gcccaattct tttgcggtgc ggtagaggat gccgcgccgc
    22261 aggcggctgc acagcgaaca agtcgttttg ccttcgtcca acacgcgttt gacggtggag
    22321 taagtgtctt cttcaacgat tttgtaggga acgccgatgc tttcgagata ggtcggcaat
    22381 acttcttcgg ggaagcccgg ctgtttttgg tcgagattga cggcgaccag ttcaaaatca
    22441 atcggcgcgc tggcttggag ccggcgcagg atgtctaaca gggcgtagct gtctttgccg
    22501 ccggagaggc agaccatgat tttgtcgccc ggctcgatca tgttgaaatc gttaatcgtg
    22561 tcgccgacgg cgtggcgcag gcgtttgctg agtttgttgt tttcgagttc ttgcttggtt
    22621 tttttggaca tggcggtggt ttgaaaaatt agggatgcgg cattgtaacc gaaaacaggg
    22681 gcggcaatgc cgtctgaagg gcttcagacg gcattagagg gcttattttg catttttggt
    22741 tttaaagaag agatgaaccg ctttgaagat accgccgttt gggacggtga gttctttttg
    22801 cgcggcggag aatttaatca cggcaagggc ggtaaagttt tccgaatctt gaacgctgtc
    22861 gagtacgatg ccggcttctt cgccgtccgc cgtcagcagg atgcccgctt cggctgccga
    22921 atttcccgac aataccgcca agccgcgttt gacctgcccc cgatactggg cgcgcgcgat
    22981 gatttcctgt cccgggtagc agcctttttt gaagtgtacg ccgccgatga tgtgctggtt
    23041 gagcatttgg gcgacggcgg tttctttggt agccgcgcat atccacggat aaccgctgcg
    23101 gatttcgtgc agccgccacg cgttttcggc ggcagcgtca taagggggta gggcggtttc
    23161 gggggcgatg tgcaaaatgc cccgatggtg caggatgacg gaacagatgc cgtctgagac
    23221 gcattcggag gtaaaggcga gactgggttc ttgcgcggca agcggttcgg cggatgctgc
    23281 caattccgcg ccgacggcgt aatcttcaag gatttcaaaa acggctttgg cgcgcaacac
    23341 aaacatccgc aggcgtttga ccgttgcttc aagcaaatct tgcgccataa tcagcagcaa
    23401 atcgtctcca cggttgacga caatcatatt ggcgatgacg cggcctttgg gcgtgttgta
    23461 agtcgcgtaa cacgcctgcc cggcctgaag attattgatg tcgttggaaa gctgtccgtg
    23521 caggaaggtt tggcggtctt cgccgctgac gcgcgccacg ccgaaaaagg gcagtaaggt
    23581 tttcatcatt tgcgtactct gaaaatataa ggaaatctgt ttatgcagtt gccgcgtctc
    23641 tcttcacggc ggttattttg atttcgacgc gccactccgg acgggcgagc cgcgcttcca
    23701 cgcaggcgcg ggcgggcgtt ctgccggcgg caacccaagc gtcccacacg ccgttcattt
    23761 ccgcatagtc gcccatatca cgcaggtaga tgaccgcatc caaaacgtgt gccttgtccg
    23821 agccgcattc cgccagccag cggtcggttt gggcaagcac gtcggcagtc tgttcggcag
    23881 ccgtttcacc gttttcggga accatgccgg agaggaaaat caagccgccc gcgcaaaccg
    23941 cttcggaata gcggggcgtt gtgccgaaat accggatatc catatcggtt tccttcgata
    24001 aaggggatat atggtaacat tgcgcttgac cgatttccat gttttgcatg acgaaaaatg
    24061 agtaaacaca cttatccgat aacacctgcc gtacgcgttt tgcgtgaaaa cggcatcgaa
    24121 tttgaacctt ttacctatgc ctatgaggaa cacggcggca cggcgcagtt tgcccgactg
    24181 ttcggcaaag acgaacactt ggtcattaaa accattgttt tgcaagatga aaacggtaag
    24241 gggccgattg tcttgatgca cggcgacaaa cagatttcaa cccgcaatct ggcgcggcat
    24301 ttgggtgcga aacacatcga acccgccgcg cctgcgcagg caaacaagtg gacgggctat
    24361 ctggtcggcg gcacaacgcc gttcggcatc cggacaaaat tggatattta cgtcgaacag
    24421 tcggtgatgg atttggaaac catctatatc aatggcggaa aacgcgggtt cattatcggt
    24481 atccgccccg atgatttaaa tattttgaac ccgaaaacaa tacaggcagc ggtttgaccg
    24541 ggaagtataa ggagcaatat ggacaaagat ttgtatgccg tattgggcgt gtcgccgcag
    24601 gcgggggcgg acgaaatcaa acgcgcctac cgcaagctgg cgatgaaata ccatcccgac
    24661 cgcaatccgg gcaatctgca ggcggaagaa aagttcaaag aaatccaacg ggcttacgat
    24721 acgctttccg acctgtcaaa acggacgcaa tacgacgcgt ccttcagacg gcatgaggaa
    24781 cgcgggcggc aggaagaggc attccgccgc gaacaggcgc gcagggagca gttttacggc
    24841 gaacagatgc gccgcgaaca ggcgttcaga cgggagtttg aacggcaggc ttcacgttcg
    24901 cgccatgctt acgaaccgtc cggcagcgga agcgggcgca actatatcct cgccgcctac
    24961 atcctgttcg gtttgggtgc aatcatacag ttcatgccca tagtcggcgt gattctcgcc
    25021 tatatgaaac ggaacagttt ggacagcatt gtctatgccg cacataccga atacctgatt
    25081 aaaacctttt ggcgcacatt ttggctttat cttttgggcg cgctgactgc ccgtttcggc
    25141 atcggcgtgc tggttattat tgcaacgacc gtctggtatt tctaccgcat catcgccggc
    25201 tttatccgct tcaacgacgg cagggcggtt gcgcccgaga aatggatata gagcatggcc
    25261 tacctgttaa tcagcatcgt gttcagcgtg tcggtttcca ttttgctgaa aatggcaagg
    25321 aagaaaaaaa tcgacatcgc gcaggcggtc gccgtcaatt atgtggtcgc ggtcatactg
    25381 accctgtcgg tattgaagcc ggatgtcggc aatatcggtg catttttgcc gacgtggccg
    25441 ctgtttgccg ctttgggcgt gctgctgccg tccgtatttg tcattatggg caaatccgta
    25501 gaatccgccg gtatcgtcaa atccgacgcg gcgcagcgtt tgtcgctgtt tttaccgatt
    25561 gttgccgctt tgacgctgtt tggcgaaaaa ctcagtgaag gcaaactgat cggactgtgc
    25621 ctcgcctttg ccgcactgtt ctgcctgctt tggaaaagcg acagcggcaa aaaatcaggc
    25681 agcgcgtggc ggcaggcgtt attgctgctc ggtgtgtggg cgggttacgg cattatcgac
    25741 atcctgttca aacagattgc caaaagcggc acggcatttg cgggcaacct gctggttgca
    25801 tttgcgctgg cgggtgtgct gatgtttgcc tgcctgtttg ccaaatcggt cagatggcgc
    25861 gttgagagcg tggtcggcgg catactcttg ggcggtttga attttatgaa catcgtaacc
    25921 tacatcactg cgcaccaaat gatgaaggac agtcccacgc tggtttttgc cggtatgaat
    25981 atcggtgtga ttgttttagg gacgctttcg ggcgcattgt tctttaagga aaaaatcaac
    26041 acaatcaata cggcaggcat tgtactgtca ttatgctcca ttgcctgcct gttctattgg
    26101 acagaagtta aggcactgtt cggcatataa tccggctgat tgatataaca aaatgccgtc
    26161 tgaaacagga aaatgctgct tc
//
LOCUS       DAWTHD010000015        24206 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWTHD010000015 DAWTHD010000000
VERSION     DAWTHD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24206
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.015"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            101..325
                     /locus_tag="WM704_000517"
     CDS             101..325
                     /locus_tag="WM704_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340005.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            381..1037
                     /locus_tag="WM704_000518"
     CDS             381..1037
                     /locus_tag="WM704_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFA9340006.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIGGNTVLLADPSPGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE
                     "
     gene            1165..2460
                     /gene="hisS"
                     /locus_tag="WM704_000519"
     CDS             1165..2460
                     /gene="hisS"
                     /locus_tag="WM704_000519"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFA9340007.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVIQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            2461..3090
                     /locus_tag="WM704_000520"
     CDS             2461..3090
                     /locus_tag="WM704_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFA9340008.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            3240..4697
                     /gene="der"
                     /locus_tag="WM704_000521"
     CDS             3240..4697
                     /gene="der"
                     /locus_tag="WM704_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFA9340009.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            4728..5627
                     /gene="rdgC"
                     /locus_tag="WM704_000522"
     CDS             4728..5627
                     /gene="rdgC"
                     /locus_tag="WM704_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFA9340010.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            5679..6068
                     /locus_tag="WM704_000523"
     CDS             5679..6068
                     /locus_tag="WM704_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340011.1"
                     /translation="MLKHLAFLLPAMMFALPAQTAVLSPYQETGCTYEGGIGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAAGREVFLEPFNSDSTKFRNMALSGTFK
                     QGLAHGRFAASQNGETLFYYEMRTRHD"
     gene            6163..6729
                     /locus_tag="WM704_000524"
     CDS             6163..6729
                     /locus_tag="WM704_000524"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFA9340012.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6795..7499)
                     /locus_tag="WM704_000525"
     CDS             complement(6795..7499)
                     /locus_tag="WM704_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFA9340013.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            7666..8253
                     /locus_tag="WM704_000526"
     CDS             7666..8253
                     /locus_tag="WM704_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9340014.1"
                     /translation="MVSTIRTGCGRNIFPKSSTGPPTQRHQNRHPRPHVPLSAERNSK
                     HPDRSLRFCPIPAKRFGHLRMAQHVFLPRFPFIDIDRIRWVRAGLKAFKHYIRENGLP
                     DLIHAHCMNYAGILAQKISEKYGIPYVLTEHSSTITRGLIRHHQWQPMEKAAAPASAR
                     LAVSRHFAHVLQHKYGCEWQYLPNIPGGIFKQTFE"
     gene            8281..8838
                     /locus_tag="WM704_000527"
     CDS             8281..8838
                     /locus_tag="WM704_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9340015.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGIAHAVTFLGALQPEAILDLMRNSDAFILASRTETFGVVYIEALSQGLPV
                     IAIHCGGAESIVSDGNGYLVSVDDADVLAFLKIYEHHADFSAEQLRTDCLATFGEDAV
                     IGRLIAVFRQAARNTARKRPKNRLK"
     gene            8864..9817
                     /locus_tag="WM704_000528"
     CDS             8864..9817
                     /locus_tag="WM704_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFA9340016.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9922..10245)
                     /locus_tag="WM704_000529"
     CDS             complement(9922..10245)
                     /locus_tag="WM704_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340017.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(10418..11776)
                     /locus_tag="WM704_000530"
     CDS             complement(10418..11776)
                     /locus_tag="WM704_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFA9340018.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNISAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(12134..13834)
                     /gene="recJ"
                     /locus_tag="WM704_000531"
     CDS             complement(12134..13834)
                     /gene="recJ"
                     /locus_tag="WM704_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFA9340019.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(13886..14617)
                     /locus_tag="WM704_000532"
     CDS             complement(13886..14617)
                     /locus_tag="WM704_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340020.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILVYAPLVDIGCG
                     GRGAGLAYGSDCSREFLRQMNGNAV"
     gene            complement(14664..15254)
                     /locus_tag="WM704_000533"
     CDS             complement(14664..15254)
                     /locus_tag="WM704_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HFA9340021.1"
                     /translation="MQGVRCKKPSERGGILLKYAIGKNSRIKMFEQEDEWISKTQMKK
                     QMNGLQDLGMELTRLSNDTLKKIGLDEDLYEAVVTYKKITSNGALKRQAQFIGRLMRD
                     TDPAPIEAFLAKLRGDDAAHNAFLQRVERARVRLLADEGALTQFMSDFPNAGAGRLRT
                     LVRNTKKEQEQDKPPKNFRALFQELKTVMENGGAEI"
     gene            15324..16655
                     /gene="pmbA"
                     /locus_tag="WM704_000534"
     CDS             15324..16655
                     /gene="pmbA"
                     /locus_tag="WM704_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFA9340022.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(16954..17157)
                     /locus_tag="WM704_000535"
     CDS             complement(16954..17157)
                     /locus_tag="WM704_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFA9340023.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            17402..17704
                     /gene="clpS"
                     /locus_tag="WM704_000536"
     CDS             17402..17704
                     /gene="clpS"
                     /locus_tag="WM704_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFA9340024.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            17706..19997
                     /gene="clpA"
                     /locus_tag="WM704_000537"
     CDS             17706..19997
                     /gene="clpA"
                     /locus_tag="WM704_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFA9340025.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLGLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(20037..23135)
                     /locus_tag="WM704_000538"
     CDS             complement(20037..23135)
                     /locus_tag="WM704_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFA9340026.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVVVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDENFEPEAMDSSAKEFLQAVINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(23366..23989)
                     /locus_tag="WM704_000539"
     CDS             complement(23366..23989)
                     /locus_tag="WM704_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9340027.1"
                     /translation="MTIKSGGIADGYQCRLKNVVWKTLGEVAEYSKNRICSDKLNEHN
                     YVGVDNLLQNREGKKLSGYVPSEGKMTEYIVNDILIGNIRPYLKKIWQADCTGGTNGD
                     VLVIRVTDEKVNPKYLYQVLADDKFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLPE
                     QEKIVAILGKFDTLTHSVSEGLPHEIALRRKQYEYYREQLLAFPKAA"
     gene            complement(23961..>24206)
                     /locus_tag="WM704_000540"
     CDS             complement(23961..>24206)
                     /locus_tag="WM704_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9340028.1"
                     /translation="QENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQKIVKILDK
                     FTELEATLEAELALRKRQYRYYRDLLLDFDNQIGGDS"
BASE COUNT         5825 a         6763 c         6007 g         5611 t
ORIGIN      
        1 cggtttaaat ttaatccact atatatttat ccgatatatc aacatctctc tatcttattc
       61 cgaattttat tgttgaaact tgcattaaaa accatacaag atgatgaaga acaaatatat
      121 tttaataaaa gcagcaatcg gcataaccgc catatccata ttttatcttc tgactcaagg
      181 aagcatcggc aaaactgaag aaccatcata tttcttgatg ttcatgtttc tcaactcgct
      241 ttggtttgaa gaaaataaaa cagttatggc tgccgttacg gcaatgattg ccgcccactt
      301 tatatttgtc gcactatccg attaataagc aaacaaaaag gctgcttgga aatttcaggc
      361 agcctttcag gaggatttta atggaacaaa aaaggcggtt tgccgcctcc ctgctcctcg
      421 ccgccgccct gccgctttgt gcacattctt ttccctttgc ggaagaaaac cccatagcct
      481 acggcaaagt caaaatacag agttggaaag cgcggcggga tttcaatatt gtaaagcagg
      541 atttggattt ttcctgcggg gcggcttcgg tggcgacact tttgaacaat ttttacgggc
      601 aaaagctgac ggaagaagaa gtgttggaaa aactgggtaa ggaacagatg cgcgcgtcgt
      661 ttgaggatat gcggcgcatt atgcccgatt tgggttttga ggcgaagggc tatgccctgt
      721 ctttcgagca gctcgcgcag ttgaaaatcc ccgtcatcgt gtatctgaaa taccgcaaag
      781 acgaccattt ttcggtattg cgcggcatag gcggcaatac ggttttgctt gccgacccgt
      841 cgccgggtca tgtttcgatg agcagggcgc agtttttgga ggcttggcaa acccgtgagg
      901 gaaatttggc aggcaagatt ttggcggtcg tgccgaaaaa agcggaggcg atttcaaata
      961 aattgttttt cacacatcat cccaagcggc agacggagtt tgcagtcgga caaatcaggc
     1021 aagggcgcgc agagtaagtc aaatgccgtc tgaaaactgt ttcagacggc atttttaccc
     1081 gtccgcaaac catctccatc ctcacaagcc cttaaaaatc cgctataatc gcccgcaatt
     1141 tgatttcagc accccctttt tgaaatggca caaaaaatcc aatccgtcaa aggcatgaac
     1201 gaccttctgc ctgtcgggca aaaagatttc aaactcacgg ctgtgttttg gcaggcgttt
     1261 gaggatacgg taaaccgttg gacgcgagct tacggttatc agcaaatccg tacgccgatt
     1321 gtcgagcaaa ccggtttgtt tgtccgctcc atcggcgagg aaaccgatgt agtcggcaag
     1381 gaaatgtaca ccttctccga ttcaaacgat tctttgagtt tgagcctgcg tcccgaaggt
     1441 acggcctctt gtctgcgtgc ggtggtcgaa cacaaccttc tgtacaacag cccgcaaaag
     1501 ctgtggtata tggggccgat gttccgccgc gagcgtccgc aaaaaggacg ctaccgccaa
     1561 tttcatcagg tcggtatcga ggctttgggt tttgaagggc cggatattga tgcggaaatc
     1621 atcgcaatgt ctgccgactt gtgggaaaaa ttgggtattc gcgaatacct gactttggaa
     1681 atcaacagct tgggcaaccg tgaggaacgc gcggcacacc gtgcggcatt ggttgaatat
     1741 ctgacccgct atgaagcgca actggacgaa gacagcaaac gccgtctgaa aaccaatcct
     1801 ttgcgcgttt tggatacgaa aaaccctgat ttgcaggaaa tctgcaatgc cgcgccgcgt
     1861 ttggtagatt acttgggcga ggcttcgcaa aaccactatg cacgcttcaa ggcgatgttg
     1921 gacggtttgg gtattcaata tattgaaaat tcgcgcttgg ttcgcggttt ggattattac
     1981 aatcagacgg tttttgagtg gacgaccgac aaactcggcg cgcaggcgac tgtgtgcggc
     2041 ggcggacgtt acgatggttt gattgaggaa cttggcggca agcctgcgcc gtccatcggc
     2101 tttgcgatgg gcatcgaacg gctgctgctt ttggtgagcg aatacggttc gctggaagtg
     2161 aacgccgcgc ctgatgtcta tgcaatgcac caaggcgaag gggcggactt gcaggtgatg
     2221 aaatacgcac aagccttgcg cgcgcaaggt ttcaatgtaa tacagcattc cggctatcaa
     2281 agcctgaagg cgcaaatgaa aaaagccgac aacagcggcg cgcgctttgc cctgattgtc
     2341 gcgcaagacg aactggcgga cggtaccgtt acgctcaaag acatgaacgg cgcacacggt
     2401 cagcaaaccg tttccgccac cgacttgaca gatactttac aacaatggaa gaacgcataa
     2461 atggcagccc atctcgaaga acaacaagag ttagacaact ttaaatattt ttggaaaacc
     2521 acgggcaaat ggctgtttgc cctgctgatt ttggcggcac tcggctactt gggatacacg
     2581 gtttaccaaa accgtgcggc ttcccaaaat caggaagcgg cggcggtgct ggcaaacatc
     2641 gtggaaaagg cgcaaaacaa agccccgcaa agcgaaatca atgccgaact gtccaaactc
     2701 caacaaagct acccccattc catttccgcc gcccaagcca cgctgatggc ggcggcaacc
     2761 gaatttgacg cgcagcgtta cgatgttgcc gaaggtcatt tgaaatgggt gttgtccaac
     2821 caaaaagaca gcctgattca agcgttggcg gcgcagcgtc tgggcgttgt gttgttgcaa
     2881 caaaaaaaat acgatgccgc gcttgccgca ctcgacacgc cggttgaggc ggacttcgcc
     2941 cccctgctga tggaaactaa aggcgatgtt tatgccgcac aggaaaaaag ccaggaagcc
     3001 ttaaaaaact acggacaggc tttggaaaaa atgcctcaag attctgtcgg tcgcgaattg
     3061 cttcaaatga aactcgattc gctgaaataa ataccgtctg aaatgccgct ctctcttcag
     3121 acggcatttc agacaacccc atcctacggc aattcccgta aaaccatccg ccgtgtccca
     3181 acggacacct gctcgtgttc agttctaccg aacggcactt gacacagaaa gacccaatca
     3241 tgaaaccaac catcgcgctt atcggccgcc ccaacgtcgg caaatctacc ttgttcaacc
     3301 gtttgacgcg caccaaagac gcgctcgtgc atgacctgcc gggtctgacc cgcgaccgcc
     3361 attacggaca cggcaaagtc ggcagcaaac cttattttgt catcgatacc ggcggtttcg
     3421 agccggttgt ggacagcggc attttgcacg aaatggcaaa acaaaccttg caggctgtcg
     3481 atgaagccga tgcggttgtg tttttggtgg acggccgtac cggtttaaca ccgcaagaca
     3541 agattattgc cgaccgtttg cgccaaagtc cgcgtcccgt ttatttggcc gtgaataaag
     3601 gcgagggcgg cgacagggcc gtacttgccg ccgagtttta cgagctggca ttgggcgagc
     3661 cgcacgttat ttccggcgcg cacggcgacg gcgtgtatta cctgattgaa gaaattttag
     3721 aaaacttccc cgagcctgaa gccgaagaag ccgatgcaaa acatcccgtt tttgccgtta
     3781 tcggtcgtcc gaacgtcggc aaatctacgc tggttaacgc cattctcggc gaaaaacgcg
     3841 tcatcgcctt cgatatggca ggcacaacgc gcgacagcat ccacatcgat ttcgagcgcg
     3901 aaggcaaacc gtttaccatc atcgataccg caggcgtgcg ccgtcgcggc aaagtggatg
     3961 aagcggtgga aaagttctcc gttatcaaag ctatgcaggc ggttgaagcg gcaaacgttg
     4021 ctgttttggt attggacgcg caacaggaca ttgccgacca agatgcgacg attgccggtt
     4081 ttgctttgga agcgggacgc gcgctggtag ttgccgtcaa caaatgggac ggcatcagcg
     4141 aagagcggcg cgaacaagtg aaacgcgaca tttcccgcaa actgtatttc ctcgattttg
     4201 ccaagttcca ctttatttcc gcgttgaaag agcgcggtat agacggtttg tttgaaagca
     4261 ttcaagctgc ctacaacgcg gcgatgatta agatgccgac gccgaaaatc acgcgcgtat
     4321 tgcaaaccgc cgtcggacgc caacagccgc cgcgtgccgg cttggtgcgt ccgaaaatgc
     4381 gttatgccca ccaaggcggc atgaatccgc cggtgattgt ggtacacggc aattcgctgc
     4441 acgcgatttc cgacagctat acgcgctatc tgacccagac cttccgcaaa gccttcaacc
     4501 tgcaaggcac gccgttgcgg attcaataca atgtttcgga aaacccgtat gaaaatgcgg
     4561 aagacaaacc gaagaaaaaa ccgctgcgcc gcgtcagcct gagcaaccgt attgagaaac
     4621 gcgaaggccg aaaggaagag aaaaaccgct tcaagaagaa aaccaaagtc agtgtgaaaa
     4681 aacaattcag caaataattc ccgacatttc aaacaatagg aaacattatg tggttcaagc
     4741 agattagttt ttatccgctc aacaaagaaa agctgcctga ggcggacgta cttgccgaca
     4801 aacttgctga agctgaattt acccattgcc aaggtttgga ctggttcagc gaaggcttta
     4861 ccgcaccggt ttctttttcg cccgaactcg ttttccctgc cgactttacc ttgcgcgtcg
     4921 ccctgaaaaa agaggaaaaa gtcctgcctg ccggcgtcat ccgcgatatt ttggaagaga
     4981 aggtagcgga aatccaaaac aatgaagccc gcaatgtcgg tcgcaaagaa aaacaagagc
     5041 ttaaagagca aattacagac gacctgctgc cccgagcgtt tacccgcagc agccgtacag
     5101 aagcggtgtt taacacccgc cacggctacc tgctcgtcaa taacgcggct tccgccaaag
     5161 cggaaaacat cctgaccaag ctgcgcgaag ctttgggcgg tttggaagcc tcgctgccga
     5221 ataccaaaca atcgccctct tccctgatga ccggctggct gttgcaaggg cattgcgaag
     5281 gcggttttga attagacagc gattgcgaac tcaaaggtac gggcgatatt gttcccgtcg
     5341 tcaaagtatc caaacaagac ttaaccgccg acgaagtggt tcaacacgtc aaaaacggta
     5401 aaaccgttac ccggctcggt ttggtttggc gcgaacaaat cgcctttatc ctcactcaag
     5461 acttcacact caagcgcatc caatacctcg acgtattgca ggaagaagcc gaaagcaacg
     5521 gcgacgatgc cgccggcctt gccttcgcct cgcaaatcct gatggcggaa tccgtcagca
     5581 ccatgttgga agagctggtt tcctatttgg gcggctggca agattgattt ttcagacggc
     5641 ttttataccg tcagccaaaa tccaatggaa taacccttat gcttaaacat ctcgcattcc
     5701 tactgcccgc catgatgttc gccctccccg cccagaccgc cgtcctaagc ccctatcagg
     5761 aaaccggctg cacctacgaa ggcgggatcg gaaaagacgg gcttccttca ggcaaaggca
     5821 tatggcgttg ccgggatggg cgcggttata ccggttcatt caaaaacggc aaattcgacg
     5881 ggcaaggcgt ttataccgtt gccgccggcc gcgaagtatt tctcgagccg ttcaattccg
     5941 acagtaccaa attccgcaat atggcattgt cgggcacgtt caaacaaggc ttggcacacg
     6001 gcaggttcgc cgcctcgcaa aacggcgaaa ccctctttta ttatgaaatg cgaacacggc
     6061 atgattaaag aagtgaaact gcccaaaaac aaataacccc ccgtttttca aacagcatcc
     6121 cccaatgctt tatccgaaca ccgcaaaaaa caaggaaacc ccatgagcat caagtccgac
     6181 aaatggatac gccgaatgag cgaagaattc ggcatgattg atcccttcga gccgaaccaa
     6241 atcaaagaag ccgacggaca gcgcatcatc tcctacggta cgtccagcta cggctacgat
     6301 atccgctgcg ccaatgaatt taaaattttt accaacatca acagcaccat cgtcgatccc
     6361 aaaaacttcg acccgaaaaa tttcgttacc gtcgaagacg actgctgcat catcccgccc
     6421 aattccttcg cactggcgcg cacggtcgaa tatttccgca ttccgcgcaa cgtcctgacc
     6481 gtctgtttgg gcaaatccac ctacgcccgc tgcggcatca tcgtcaacgt taccccgttc
     6541 gagccggaat gggaaggcta cgttaccctc gagttttcca acaccacccc cctgcccgcc
     6601 aaaatctacg caggcgaagg cgtggcgcaa gtcctcttct tcgagagcga cgaagtgtgt
     6661 gaaacttcat acaaagaccg caacggcaaa tacatgggac aaaccggcgt aaccctgccc
     6721 aaagcctgaa atacaaaatg ccgtctgaac ccttatcgtc gggtttcaga cggcatctac
     6781 ctatttatcc gtggttagaa ctgaaccgtc ccattgatgc tgatgctgat ttcctccaca
     6841 cccggtgcgg ctgaatccgt acccttcata ttgacgcttg ccgccatcgg catcgcgcgc
     6901 agcatttttg cccgaacagc cccatcgccc gcaatatggc tgccgatttg cccaaaattc
     6961 aatttgacga ttttataacc ggacgcaccc agaacgcccg ccagtttttc ggcacgcgcc
     7021 ttgaaacgca aaacggcatc cttgctgacc tgatcgatga cctcgtttcg gcgttcgcgc
     7081 gacacgctga aatcggtatc ttcaagcgaa gcatccgtct gaacatcagc aataaaacgg
     7141 tttaaagcat caaaatccct gccctccgcc ttaaattccg cacgctcctc ccagcctgtt
     7201 tgaatgcgtc tgccgttggt atattgatag cgcggcatcg cactgcgcga taccaattcg
     7261 gttttaaagc taccattttt cgattttctg gtaaaattgt tgaatttttt aacaaactcg
     7321 gcattgacgg catttttgtc ccgtccttcc gccgccacct ggaaacgcgc ggacattgta
     7381 tcctgagcca cctcgatacc cgccgattcg gaaaattcga caatattgta attcaatgcc
     7441 tcagccgccg ccggaaaaga taccgccagc agggaagccg ccaaaacaga acgcagcata
     7501 tccgtatcct tacttgaaaa accgccaatc taacatatag gtacgctaaa ttgcgttagc
     7561 ataagacaac aatttaaata ttcgatcggt ctgccttata ataaccgtct ttccgatact
     7621 gaatcatatt atgtccgaac cgttgcacgt cctcgtcatc cccttatggt atccacgatc
     7681 cgaacaggat gtggacggaa tatttttcca aaatcaagca caggccctcc aacgcaaagg
     7741 catcaaaacc gccatcctcg cccccatgtt ccgttatctg cggaaagaaa cagcaagcat
     7801 cctgaccggt ccttacggtt ttgcccgata ccggcaaagc ggtttggaca tctacgcatg
     7861 gcgcagcatg tatttcttcc ccgctttccg tttatcgaca tcgaccgcat ccgctgggtg
     7921 cgcgccggtt tgaaagcctt caaacactac atccgcgaaa acggactgcc cgacctgatt
     7981 cacgcccact gcatgaacta tgcaggcata cttgcccaaa aaatttccga aaaatacggc
     8041 attccctacg ttttaaccga acacagcagc accatcacgc gcggtctgat ccgtcaccac
     8101 caatggcagc cgatggaaaa agcagcggcg cccgcctccg cacgtctcgc cgtcagccgc
     8161 catttcgcac acgtcctgca acacaaatac ggctgcgaat ggcagtacct ccccaacata
     8221 ccgggtggaa tattcaagca aacatttgaa taaaaagaaa aaaataacaa accgcatttc
     8281 gtgttctgca ccgtctcgca ccttcgccgc ctcaaaggac acgatgtcct gctcactgct
     8341 tttgcccggg cgttggcaca atgcccgcaa ctgcgcctga acatcggcgg cagcggacag
     8401 gaagaacagc ggctgaaaca gcaggcggca gacttgggca tcgcccatgc cgttacattt
     8461 ttaggcgcac tgcaacccga agcaatcttg gatttgatga ggaacagcga cgccttcatc
     8521 ctcgccagcc gcaccgaaac cttcggcgtg gtctatatcg aagccctctc gcaagggttg
     8581 cccgtcatcg ccatacattg cggcggcgcg gaatccatcg tttcagacgg caacggatac
     8641 ctcgtttccg ttgacgatgc cgatgtccta gcctttctca aaatatatga acaccatgcc
     8701 gatttttccg ccgaacagct cagaaccgac tgcctcgcaa cattcggcga agatgccgtt
     8761 atcggcagac tgattgccgt attccgtcag gcggcgcgga acacggcgcg aaaacgcccg
     8821 aaaaatagat taaaatgacg caaaacaacc ttcggattca aatatgaccc ataccgtcca
     8881 cctgcacttg gaagaaaccg acaacccgac gctgcaacgc ctgtgcggtt ctttcgacag
     8941 caacctcgat tcgctcgcca aagcactcga catccatatc agccgccgtt tcgagcattt
     9001 taccttcaac ggtgcatttg cacacgccgg caaacgcgca ctgctcaaac tcttggaaac
     9061 ggcgcagacg cgcgacctaa acgacagcat catccgcctt gccgccgtcg aaacccaaac
     9121 cgaagatgcc gggcatcagg aaaaaaatca cgaacacgcc tattatttcc gaaccaagcg
     9181 cggcagcata ggcggcagaa cgccacggca gaacggctat atccgcgccc tgctcgacca
     9241 cgacatcgta ttcggcctcg ggccggcagg tacgggcaaa acctatcttg ccgtcgccgc
     9301 cgccgtcgat gcgatggaaa aacaccaagt cgaacgcatc attttagtgc ggcctgccgt
     9361 cgaagccggt gagaaactgg gcttcctgcc cggagacctg acccaaaaag tcgaccccta
     9421 cctccgtccg ctttatgatg ccctctatga cctgatgggc tttgaccgtg taaccaagct
     9481 gattgaaaaa ggcctgattg aaatcgcccc gctcgcctat atgcgcggca ggacgctcaa
     9541 cggcgcatac atcatcctcg acgaagcgca aaacaccacg cccgaacaaa tgaaaatgtt
     9601 cctgacccgc atcggcttcg gcgcgaaagc cgtcattacc ggcgacacca gccaaatcga
     9661 cctgcccaaa aacatcaaat cgggattgaa agacgcacgt gagaaactgc acggcgtggc
     9721 aggactgtat ttccatacct tcaccggcga agacgttgtc cggcatcctc tggtgcaaaa
     9781 aatcgtcgaa gcctacgaat cggcagaaca cgactgacac tccaatgccg tctgaaaccc
     9841 gatgcatgga aaatcgggca gagaaaaaca gagacggaag ccaagctccg tatccgtttc
     9901 tttatccgtc caaccgatac atcaatcgaa ttttctgcat cggattgcct catctccgca
     9961 aaacattgcc gaagtttcgc ggttgcacgc ctgacgcact ttctgccgcg ccacgccttc
    10021 attccgatcg cttgcctcaa acgtcttctg aaaaggcgta acgctgcata catacatggt
    10081 ggaatttgaa ccgaaaaggt cgattttgac ggtattgccg ccgctttcca atacggcgac
    10141 acggtgttcc aactgcgcca gacgtgcttc caaatcgctg attctgtcgg caaatgcagg
    10201 catggcggca cacgcggtca aaatgcccaa aatacatttt ttcatcaatt tttccttgtt
    10261 gcttgaaccc tgcccacccg gacatccttt ttccgggcgg cagggtcaga ctttatttgc
    10321 agggatgaag ccccttacaa atcagaataa tacatgaatc ggcactttat gcttcatcct
    10381 atgtgttgaa acttcattgc tccttgtctg ttgaccgtta ttcccaatcc gttcccacaa
    10441 ccttcggctt gggtttgcgc ctgcgacggc ggcgtttttt cgcctgtcct tcgttttttt
    10501 cgtgccgggc ggcggcctcg tttttggtca tttccgtccg ctgttccgcc gatgccgtct
    10561 gaaacgccgt ccaccactcg gcaagtgcgc ggtccgcatt gccggtttcg gcgcgcaaga
    10621 gcaggaaatc ataggcggca cggaaacgcg cctgtgcaaa cagtttgtgc ggacgtgcgc
    10681 ctttgcggtt ttcaaactgc ggctggaaca tccaaatttc acgcatcgtg gcggaaaagc
    10741 gttgcggtac gccccaaccg cgttcgacgg tttcgcgcat cgtattgatt gcatcggaca
    10801 gggcgggcgt aggtttcaaa ccctgttgca gattgctttt ccaatggcgg tccaactctg
    10861 gccacatcag ggccgccagt acgaaaccga ccgaaaccga tttgtcggca cgcagccgct
    10921 catcggtatt tttcagggca agcgccgtca tttttccggc gatgccgtct gaaacgcgca
    10981 aagcattgag cagcggatgg atgtccggta tgtcaaatcc gttcaaacgt ttcagacact
    11041 cgcgcgcgtg ccctgaaaac agcagtttca tgatttcgtc gaacagcctt gccacaggtt
    11101 cgtgcttcag acggcatatc gattcggcaa tcggtgcggc ggtttcttcc gacagctcaa
    11161 agcccaattt gcccgacagg cggatggcgc gcaaaatcct gacagggtct tcctgatagc
    11221 gttcggcggc attaccaatc ataaccagcc tgcgggcggc aacatcggca atcccgttgt
    11281 ggaaatccaa aatctcttct ttttcaggat cgtaatacaa ggcattgcag gtaaaatcgc
    11341 gccgcatcgc atcttcttcg atgctgccgt aggtgttgtc cttcatgatt ctgccgtgcg
    11401 cgttctgatg aaccttcgca ccgccgcgaa acgtcgttac ttcgataatc tccgcaccgt
    11461 tcatcacatg gacaatctga aaacgcctgc cgatgatgcg gctgcggcgg aagagtttgt
    11521 gcacctgttc gggcgtggca tcagttgcga catcgaaatc tttgggttcg atgccgagca
    11581 gcaggtctct gaccgcaccg ccgaccacat aagcctgaaa ccccgcccct ttcaggcggc
    11641 gtatgacgtt ttcggcggca aagctcaaca tttcggcact gatgttgtgt ctttcggcag
    11701 gaatgaccgt tttactttcc gcttttttac tgctccgacc ggaaggcagc atcttattca
    11761 accatttttt cagcataaat attctgaccc gaagagcggc atctccacgg gcaaccgtgt
    11821 tcagactgca tcaggcggta cgattccaca agatgccgaa aaccataagc aaaaggcgta
    11881 gaaactacgc cgtccgaatc acgccgccct cgcggcggca acgcgtcatt ataacagatt
    11941 gccccttaaa aaatcagacc ctgcttttgt ggcggagtct gaaatttgat gcaccttata
    12001 tattgcgtcc ctaagaaggg atgattaaca aaaattaaca ccctttactt tcttttccga
    12061 ttgggaacat tcccgtcgta caaacaagca aatgaaggaa cagaaatcac attcaaacgg
    12121 cgttccgccg cctctacgcc gcttcccagt agtcgatata aagctgcaat tcgaggttgt
    12181 cgcgccattc gttggcaacg gggcggtaca ccgtgcggat gtattcggga atgtcttcgc
    12241 tgcaacgcca gaacatcgct tcaaattcgc agccgtcttt ttgcaaccac gcttttttgt
    12301 gtttgccttc cgcgcccaaa ggctgctggc gaacgacgtg gaactcgtcg gtaaagctcg
    12361 gcggcgcaaa gccctgcccc caaacgtggc aggcgaggtt ttgcgcctgt tccaacgtga
    12421 tgtcgcaggc gggcaggctg ccgtcagtaa tataggtttg cgacaaatcg tcttcgcaca
    12481 ccatctcacg cacggcttct tcaaaggccg tctgaaacgc gggaatattg tgttcaagta
    12541 tgcttaaacc cgccgccatc gcgtgtccgc cgaatttcaa aatcaaatcg ggatggcgtt
    12601 tggacaccaa gtccaaagca tcgcgcaagt gcagattggg aatggagcgt cccgaaccgc
    12661 gcacttcgcc gttgtccgcc ggcgcaaaca cgatggtcgg acggtaaaaa cggtctttga
    12721 ggcggctggc aacaatgccg accacgcctt gatggaagtc gtcgcgatac gccaccaaag
    12781 tcgtctgacc tgaaggcagg gtttcgggaa aggcgttcaa cgcgtctcgc agcatagact
    12841 gctcgatttc gcggcgttcg atattgaggt tgtttaaccg agccgccagt tcctgcgctt
    12901 cggaatcatc tcgcgccaac aggcaggcga tgccgaccga catatcgtcc agccgtccgg
    12961 cggcgttgat gcgcgggccc aacgcaaagc ccatatcaaa cggctgagcc ttgcgccaat
    13021 cgcgccgcgc cacttcaaac aaggcgcgga tgccggggcg cattttgccc gaacgcatcc
    13081 gtttcaaacc ttgcgacacg aggatgcggt tgttgtggtc gagggggacg acatcggcga
    13141 cggtaccgag tgcgaccaaa tccaaaaggc cgcccagatt cggctctttg atgccgtctg
    13201 aaaaataatt gcggcggcgc agttcggcac gcaacgccgt caacacataa aaaatcacgc
    13261 ccacgcccgc caagcttttg cttggaaaac cgcagccttt ttggttcgga ttgacgatga
    13321 tgcagtcggg tacggtatcg gctggcaaat ggtggtcggt aacgatgaca tccaaaccca
    13381 gagcctgcgc gcgcgccacg cctgcaatac tggcaatgcc gttatccacc gtaatcagca
    13441 aatccacgcc ttgcgcggcg gcgatttcgg cgagttcggg cgttaagccg tagccgtgct
    13501 caaagcggtt gggcacgagg aaatccactt tcgcgcccat cgccgccaaa ccgtccaaac
    13561 cgacggcaca cgcggtcgcg ccgtcggcat catagtcggc gacaatcagg attttttcct
    13621 tgcgttcaac cgcatccgcc aaacggccgg cggcggcttc gcaattcgtc agcgattgat
    13681 aaggcaggag ggaagcgagt ttgtcgtcca actcggcagg actttgcaca ccgcgcgagg
    13741 cacaaagccg ggcgattaaa ggatcggcgc cggcggcgag caaatgattg aaaacgtcgg
    13801 tattgacggg gcgggtttgg attttgactg acatagcgcg gggttttaca gtgcgggtgt
    13861 gcttgaaatg ccgtctgaag gcttttcaga cggcattgcc gttcatttgt ctgaggaatt
    13921 cgcggctgca atcagagcca taggcaagac cagcaccgcg gccgccgcat ccaatatcaa
    13981 caaggggggc gtatactaag atatttccaa acagcttgga tttgtcggta tgtttttcag
    14041 taaccgtata ataaatatcg gcgggcacac tttgctcaaa atgataatcg gcgttcagtt
    14101 tttgcggtgt ggcgtagtat ttgcctttgg cggatacgca gcgcgtgtaa atggtccgat
    14161 tgtcgagttt gaccgcttta aactcaagct gtttcagctt ggcgatgtcg tcaggtctgc
    14221 cggtatcata gcgcaggcaa agacctccgg tactgaaatt ctggctgccg ggcgcttcga
    14281 atttgaccgg cagggcttgg tggcgggcat agctcggggt atcctcaact atttggaagg
    14341 gcttgtccaa cccggccttc aaaaggcccg tcagcttcgc cgaatcttcg ggattgacgg
    14401 cgaaccagta tttcccgccc atcatcacca ggctgccctt ttccaattgg gcattgtctt
    14461 cggcaaccac accgaaggcg cggatttggt ctttgtcaac gtgtttgcgg gtgattgttt
    14521 ggctgaccgg gttgttcatc ccccgcaaca tcatcgtaca gccgttcagt atcaaggttg
    14581 ccgccaaaac ggcggcggtc gttttacgga acaacatttt atcttccaat ctaaataacg
    14641 gcggatgccg tctgaaaata tgcctaaatt tccgcgcccc cgttttccat cacggttttc
    14701 aattcttgaa acagggcgcg gaagtttttc ggcggtttgt cctgttcctg ttcttttttg
    14761 gtgttgcgga cgagcgtcct cagcctgccc gcgccggcgt ttggaaaatc ggacataaac
    14821 tgcgtcagcg cgccctcgtc tgccaacaac cgtacacgcg cccgttccac gcgttgcaaa
    14881 aaggcgttgt gcgccgcatc gtcgccgcgc agcttggcaa ggaacgcctc gatgggcgcg
    14941 ggatcggtat cgcgcatcag acgtccgata aattgcgcct gacgtttgag cgcgccgttg
    15001 gacgtaattt ttttataggt aacgacggcc tcgtacaaat cttcatccaa accgattttt
    15061 ttcagcgtgt cgtttgagag cctggtcagt tccataccca aatcctgcaa accgttcatc
    15121 tgctttttca tttgggtttt gctgatccac tcgtcttctt gttcaaacat cttgattctc
    15181 gaattttttc caatagcgta ttttaacaga atgccgccgc gttcagacgg ctttttgcac
    15241 cgcacgccct gcaagcggca cgcttttgcc cctgccgccc gaaacggtta aaatgccgca
    15301 acgctccacc aagaaagatt gttatgctgt tcaaccacac cgcttccgaa ctcctcgacc
    15361 tctgccgccg cacgctcgac ttggcaaaag cagcgggcgc gaccgccgcc gaagccgact
    15421 tcagcgaatc gctcggacaa agcgtcagcg tgcgcttggg cgaaatcgaa caaatcgagt
    15481 tccagcagga caaatcgctg gacattaccg tttacgtcgg caaacgcaaa ggccgcgccg
    15541 gcaccgccga cttctccgaa caagccctgc gcgacaccgt ccgcgccgcc gccgacatcg
    15601 cccgccacac cgccgaagac ggttgcgccg gacttgccga ccccgagttg atggcgcaac
    15661 acatcggcga ccccgacctt taccacgaat gggatttgga tacggaagcc gccgtcggct
    15721 tggcaaaaca atgcgaacag gcagcgttgg acacggacag ccgcatcgaa aactccgaag
    15781 gcgcggcggt gcaaaccgga cactaccaat acgtttacgg caacacccac ggttttgccg
    15841 cacaccggca aagcacgcac cacagcattt cctgcagcgt cgttgccgcc gacgaaaacg
    15901 gaatgcagcg cgactactgg tacgattccg cctgccgcca tccggatatg gacagccccg
    15961 aaaccatcgg tcaaaccgcc gcccgccgca ctttgcgccg actcggcagc cgcagcatcc
    16021 cgacaggcag ctaccccgtc ctcttcgata ccaccgtttc gggcgggctg atcggacacc
    16081 tcgtcggcac actctccggc ggcgcgctct accgccaaag cagtttcctg atcgacagca
    16141 tcggcaaaaa agtcctgccc gatttcctca acctgcgcga agaaccgcac atcccccgct
    16201 ctttccgcag cagctatttt gatgcggaag gtgttgccac cgcgccgcgc ttcgtgattc
    16261 aaaacggcat tgtcgaaggc tatttcctcg gcagttacag cgcgcgcaaa ctcggaatgc
    16321 agaccacggg caacgccggc ggcgcgcaca acctgtattt gaaccatacg cacgaaacgc
    16381 aatccgacct gctgaaagaa atgggcacgg gattgctggt aaccgaattg atgggacagg
    16441 gtgcgaacac cattaccggc gactattcgc gcggcgcggc gggtttttgg gtggaaaacg
    16501 gcgtgattgc ctaccccgtc cacgagatta ccgttgccgg acgcttgcag gatatgtacc
    16561 gcgacatcgt cggcgtggcg gatgacgctt tgcggcgttc gtccaacaaa atcggttcga
    16621 ttctgattgc ggggatgacg gttgccggaa gctgaacgcc ctgccgatgc cgtctgaaga
    16681 cgaaggcggc gcgttttgcc ccgtgcctgc ccgtttgccg ttttatgccg caccgccttt
    16741 taccggcgac attgcacatt tttttcatca gcactgattt tcccttatcc gtcaaaatac
    16801 taagccactt gcccacaaca gggtacacac aagttcagga taaggcgggc ggggattggg
    16861 gacttttccg ccatcggcaa aacggcagac aaaaacggcg ggacattgcc tgtcccgccg
    16921 ttttcaaata tacatttgac cgtacatcag gaattaagct gcctgaatgt tggcggcctg
    16981 tttgccttta gggccggtgg ttacgtcgaa agagacgcgt tggccttctt tcagggtttt
    17041 gaaaccttcc atattgatcg ctgagaagtg tgcgaacaaa tcttcgccgc cttcgtcagg
    17101 agtgatgaaa ccaaaacctt tagcgtcgtt aaaccatttt acgataccgg ttgccattag
    17161 aaacttccta tacttaaaaa ttaacaaaat cagcaaaata aggcatacag caaacttaaa
    17221 cgttcagcgt ttccacccag ctttatatac ttggtcgagt gccgactttt gcttaaccgt
    17281 ctttttacat ttgttaagac aaaacgtcaa gctatgtttt caaaaaagtg ggaaaatagg
    17341 cgaaagtacc gaaaatacaa caaaaatgcc gtctgaacgc ggctttcaaa tgggacacgc
    17401 tatgaccgcg caacaccaat ccgacacgct tttgcaccgc ctgaacaccc tgccgccgaa
    17461 acgttacggc gtatttttat tgaacgacga ttacaccacg atggaatttg tcgttgaaat
    17521 cctgaccgaa atctttatgc tcgggcagga acaggcggta gcggtaatgc tctcggttca
    17581 tcacgaaggc aaaggcctgt gcggcactta cacgcgcgat atcgcccaaa ccaaacagca
    17641 acaagtcatg cagcgggcaa aagccgaagg acatccgctg caatgtattg tcgaggagat
    17701 ttaatatgct tgcccctgaa ttggaacaga ttttgcagca gctttaccgc gaggcgcgta
    17761 aggctcatta tgaatttatc agcctcgagc atctgctttt ggtactcatc gaagaagatg
    17821 ccgccgtgcc gaacgtctta aaactctgcg gtgcggattt aaaagcggtg tccgaacagc
    17881 tcgccgccag cgttgccgaa aacacccccc tgattcccga ccacctttta gacacggtcg
    17941 aaacccggcc cacgctcggc ttccaacgcg tcatccagcg ggcgatggtg catacccaat
    18001 cggcaggcaa aggcttggcc gagcctttgg acgttttggt cgcgctgatg agcgaaaccg
    18061 acagccacgc cgtttatttt ttgggtttgc aatctgttac gcgttttgaa gttttgcgct
    18121 gtattgccca cggctctccc gatgaagatg aagacgatgg caactatttt tcagacggca
    18181 tggacgacga taatgagaac cgcaccaaac cgggcaaaaa ccctttatcg gcgtacaccg
    18241 tcaacctcaa cgccgaagtc aaagccggcc gcatcgaccc tttgattggt cgcaaacacg
    18301 aaatggaacg gctggtgcaa atcctgtgcc gccgccgcaa aaacaatccg cttttggtcg
    18361 gcgaagcagg cgtgggcaaa accgcgctgg cggacggttt ggcacatcaa atcgtcaacg
    18421 acgacattcc cgacgcgctc aaagaggccg aagtgtacgc gctggatatg ggttcgcttt
    18481 tggcgggcac aaaataccgc ggcgactttg aagcgcgggt caaatccgtc ttgaaacagc
    18541 tcgaaaaaat cccgcacgcc attttattca tcgacgaaat ccacaccatc atcggcgcgg
    18601 gcagcaccgg cggcggcacg atggacgcgt ccaacctgct caaacccgca ttggcgaaag
    18661 gttcattgcg ctgtatcggc gcaaccactt acgacgaata ccgtactatt tttgacaaag
    18721 accacgccct aagccgccgc ttccagaaaa tcgacgtggt cgaacccacc gttgccgaaa
    18781 ccgttcaaat cctgcgcggc ttgaaaccga tgttcgaagc cttccaccaa gtccgctaca
    18841 cgcaaggcgc actcgaagcc gccgccgaac tctccgcccg ctacatcaac gagcgcttcc
    18901 tgcccgacaa agccatcgac gtaatggacg aagcaggcgc ggcgcaacgg attctgccca
    18961 aatccaaaca gaaaaaagtc atcggcaaag cgcaaatcga aaccgtcatc gccaaagtcg
    19021 cgcggattcc cgaaaagacc gtgtcgcacg acgacaaaca agtgttgcaa ttcctcggcc
    19081 gcgatttgaa caatatggtt tacgggcagg aagacgccat cgacgcgctg gtttccgccg
    19141 tcaaaatgtc gcgttcgggc ttggggctgc ccgacaaacc gataggcagc ttcctcttct
    19201 ccggccctac cggcgtcggc aaaaccgaag tcgccaaaca gcttgcctac tcaatgggcg
    19261 taccgctgca acgctttgat atgtccgaat acatggaacg ccacgccgta tcgcgactca
    19321 tcggcgcgcc accgggctac gtcggctttg aacaaggcgg ccttttgacc gaagccgtca
    19381 acaagcagcc tcattgcgtg ttgctcttgg acgaaatcga aaaagcccat ccagatattt
    19441 tcaacgtcct cctgcaagtc atggacgcag gcaaactgac cgataacaac ggtaagagcg
    19501 ccgatttccg caacgtcatc ctgatcatga ccaccaacgc aggcgcagaa agcctcagcc
    19561 gacccagcct aggctttacc gccaaacgcg agcgcggcga cgaaatgcag gcaatcaaca
    19621 aactcttcac gcccgagttc cgcaaccgct tggacgcgat catcccgttt gcacctttgt
    19681 ccgaacccat catcgtcaaa gtcgtggaca aattcctgct ccagctcgaa caccggctcc
    19741 tcgacaaaaa agtcgaagcc gaattcacac cggcattgcg taaatacttg gcagaaaaag
    19801 gtttcgaccc gcaaatgggc gcgcgcccga tgcaccgcct gattcaggaa aaaatccgca
    19861 aaccgctcgc cgacgaactc ctgttcggca aactcgccga cggcggcttt gtgcggatag
    19921 actgggatgc ggcgaaagaa gaagccgtat tgaagtttaa gaaaagcaag gtcaaaataa
    19981 aaaccgcctc cgcataaaat caaaaatgcc gtctgaaagg ttcagacggc attttgtcaa
    20041 atatcggccc ctattccggc aaacttctcc acaaacgccg cgattttttg gaagacgctt
    20101 tgtttcttcg tcagatattg cgggttgagc ggactcattt tcggcagggt ttcggtcagt
    20161 tccgtaccgt tttcgctggc atagccgcgt ttgagcgagc cggtcaaata gcgtttggcg
    20221 gcggtttcgt tcagaccttc ggcggcaatc aatcctgccg cttcgtgccg catcacctct
    20281 tgcgcgtagg tgtaaaaggt ttccagtatg gcgggaacgt cgggcacttt atccaaatcc
    20341 gtatcgttga tgaaatccac aatcaggccc tctttggcgc ggtggccgat gctggcgcgg
    20401 atgatgcggc ggatttcttc caccagctcc gctttgcctt tgattttttt gtgatgctcg
    20461 aaaaccagtt gcaggatgta atccagattg atttcctgcg atttgagcaa atccacctca
    20521 aaaaccacat cgtcccagtc gatttttgac tgttctttct gttcgcctgc tttttggcgg
    20581 cgcagccagt ctcggatgtc gttataggcg gaacggtagt cctgcaccgc cctttcagac
    20641 ggcatcggca ctttccgcat ttcctgcacg tcttcgtcgc tcaggtagta tttttcctga
    20701 aacgccttca tcgcatcttc gtccgccgcg tccacattct gcaattcgcg cagcgcggca
    20761 aattcatcgt agttctgcaa tacattttcc gcccgcaggt attcgccgaa gagtttggca
    20821 aaatcttttt tgtctttttc cgtttcgatt ttgtcgggat cggggaaacg ctcgtgcaat
    20881 tcttttgcta catccagata accgcgccgt gcttcgccgg tctggctgtc ggtatagccg
    20941 ttcatgtatt cttcgtagct tttttccagc actacatttt tggtgttttt gtcgccgaag
    21001 agcgtgatcg catcaatggt tgcctgctcc aagtcgcgga agcagacaat gttgccgaag
    21061 gttttggtag catcgtaaat gcggttggtg cgagaaaacg cctgcatcag gccgtgatag
    21121 cgcaggtttt tatcgacgaa cagcgtattc agcgtcggtg cgtcaaaacc cgtcaaaaac
    21181 atgccgacca caatcagcaa gtccacttcc cggtttttca cccgttttgc caaatcgcgg
    21241 tagtagttct gaaaggcttt gctgtccgtg ccgaaattgg ttttgaaaca ggcgttgtaa
    21301 tcgttgatga cagcctgcaa aaattctttc gcgctgctgt ccatcgcttc cggttcaaag
    21361 ttctcatcga caatttcacc gacggcgttt tgctcttcat tggccgcaaa ggaaaaaatg
    21421 gtggctgttt tcaacgggtg caggctgcct gcctgttgtg ttttgaacgc ttcgtaatag
    21481 cacttcgccg catccacgct gctgacggca aacatggcgt tgaagccttt gccgcccgca
    21541 ttcaagcggt gcgttttctg cctgaaccgg ctcaggatat attgcgtgat ttcgcggatg
    21601 cgttcggggt gcagcagggc ttggcggttt tcggcggcac tcagttcctt ctcgtcctgt
    21661 tccgcttcca cggctttgaa ctgcgggcgc acatcgttgt aatccacttt gaatttcaat
    21721 actttttcat cgcggatggc atcggtaatc acataagaat gcagctcccg cccgaacacg
    21781 cccgccgtgg tttccgcgcc caaagcgttt tcgggaaaaa tcggcgtgcc ggtaaagccg
    21841 aactggcaga attttttaaa tttctttttc aggttttttt gcgcttcgcc gaattgcgag
    21901 cggtggcatt cgtcgaaaat aaatacaacc tgccggtgat aaaccggcag attatcttca
    21961 cttttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt gccgtctttt
    22021 tccaaattgc gtttcaagcc cgccgtactt tccgagccgt ttacgctgtc gggcgaaaaa
    22081 cgttggtatt ccttcatcgt ctgatagtcc aaatccttcc tgtccaccac gaagaaaacc
    22141 ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagccggt cagcgttttg
    22201 ccgctgcccg tggtatgcca aacatagccg ccgctttccg gcccgctcca attcttcgcc
    22261 tgcgccgagc tgttgatttt ccacaaaatg cgttcggcgg cggcaatctg gtacggccgc
    22321 ataatcagca gcgtatcatt cgcatcgaac acgctgtaat gcagcaaaac gctcagcaat
    22381 acgcttttct gcaggaacgt ggcggtaaag tctttcaaat ccttaatcgg atgattgtcc
    22441 gaccgcgccc aattcatcgt gaaatcgaag ctgtttttgt cgcgcttggt ggtgttggcg
    22501 aaataacgcg tgtccgtgcc gttggaaatc acgaagattt gcaggaattt gaacagcgaa
    22561 ttttcgctgt tgaagctctc tttgctgtaa cggtgcacct gattgaatgc ctcgcgcacc
    22621 accacgccgc gctttttcaa ttcaatctgc accagcggca ggccgtttac caacacggta
    22681 acgtcataac ggtttgcatg cgtgcccgtc tgctcaaact ggttgataag ctgcacatgg
    22741 ttgcgggcaa ggtttttctt gtccagcaga taaatgtttt tcagacggcc gtcatcaaaa
    22801 gcgaaatcgt aaatatggtc gtcgtggatt ttgcgggttt tatcggtaat gttttcagcc
    22861 ggcctgtcca gatattccgt caaaaaccgc gcccattcgc cgtctgaaaa cgccacatcg
    22921 ttcagccgct gcaactgcgc gcgcaggttt tccagaagcc tgctttggct gttcaaatcc
    22981 ttgcggtatt cgtacccctg attctgcaaa tcggcgatta actccgcttc caaccggttt
    23041 tccgattggt agctgcccga ctgttcgatt ttttcatatt ggtcgagcac gatgaaattc
    23101 ggcgtttcgg cgatgggttt ggtttcgagg ttcatgggtc gtcctttgcg gtgtcgggtt
    23161 ttatagtgaa ttaacaaaaa ccggtactgc gttggctcgc cttagctcaa agagaacgat
    23221 tccctaaggc gcccaagcac caagtgaatc ggttccgtac tatttgtact gtctgcggct
    23281 cgccgccttg tcctgatttt tgttaattca ctataaacgg tttgatgttc ggattgggct
    23341 gcctgaaaaa gtgcaggctg ctttttcaag cagccttggg gaaggcaagc agctgttcgc
    23401 ggtaatattc gtattgtttg cggcgcaggg cgatttcgtg cggcaggcct tcgctgacgg
    23461 agtgggtcag ggtatcgaat ttgcctagga tggcgacgat tttttcctgt tcggggagcg
    23521 gggggatggg gattttatat tgcataatcg ctgctttgct gccacgcggc atttttgcac
    23581 ctttggcgtg cttcatatta aaagcaaaaa atttatcatc ggccaatacc tgatacagat
    23641 attttggatt aaccttttca tctgttaccc ggataaccaa aacatcaccg tttgttccgc
    23701 ccgtgcagtc tgcctgccag atttttttca aatacggacg gatatttcca atcaaaatgt
    23761 cattgacaat atattctgtc atttttcctt cgcttggaac ataaccggat aactttttac
    23821 cttctctgtt ttgtaagaga ttatccacgc caacgtaatt atgttcgttc agtttatccg
    23881 aacaaatacg gttttttgaa tattcggcaa cctcccccaa cgtcttccaa accacatttt
    23941 tcagacggca ttgatagccg tcagctatcc cccccgattt gattgtcaaa atctaaaagt
    24001 aagtcgcggt aataccggta ttggcgtttg cgcagggcta attccgcttc cagcgtagct
    24061 tccagctcgg tgaatttgtc aagtattttt acaatttttt gttgggtttc caatgatggg
    24121 atggggattt tatagttatc tgtatctggt gttgctattt gtggcatttg catttttgat
    24181 ccgatattac gaaaatagtt ttcttg
//
LOCUS       DAWTHD010000016        24125 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWTHD010000016 DAWTHD010000000
VERSION     DAWTHD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24125
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.016"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(119..2398)
                     /gene="nrdA"
                     /locus_tag="WM704_000541"
     CDS             complement(119..2398)
                     /gene="nrdA"
                     /locus_tag="WM704_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HFA9340029.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKITDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            2929..3240
                     /locus_tag="WM704_000542"
     CDS             2929..3240
                     /locus_tag="WM704_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFA9340030.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(3290..3400)
                     /locus_tag="WM704_000543"
                     /pseudo
     CDS             complement(3290..3400)
                     /locus_tag="WM704_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3503..4270)
                     /locus_tag="WM704_000544"
     CDS             complement(3503..4270)
                     /locus_tag="WM704_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFA9340031.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(4358..5185)
                     /gene="mutM"
                     /locus_tag="WM704_000545"
     CDS             complement(4358..5185)
                     /gene="mutM"
                     /locus_tag="WM704_000545"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFA9340032.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(5238..5903)
                     /locus_tag="WM704_000546"
     CDS             complement(5238..5903)
                     /locus_tag="WM704_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFA9340033.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            6082..8058
                     /locus_tag="WM704_000547"
     CDS             6082..8058
                     /locus_tag="WM704_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9340034.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            8126..8818
                     /locus_tag="WM704_000548"
     CDS             8126..8818
                     /locus_tag="WM704_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFA9340035.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            9293..10663
                     /locus_tag="WM704_000549"
     CDS             9293..10663
                     /locus_tag="WM704_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9340036.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITAIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            10896..11552
                     /locus_tag="WM704_000550"
     CDS             10896..11552
                     /locus_tag="WM704_000550"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFA9340037.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            11705..13390
                     /gene="rpsA"
                     /locus_tag="WM704_000551"
     CDS             11705..13390
                     /gene="rpsA"
                     /locus_tag="WM704_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFA9340038.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            13401..13715
                     /locus_tag="WM704_000552"
     CDS             13401..13715
                     /locus_tag="WM704_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFA9340039.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(13911..14318)
                     /locus_tag="WM704_000553"
     CDS             complement(13911..14318)
                     /locus_tag="WM704_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFA9340040.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            14468..15578
                     /locus_tag="WM704_000554"
                     /pseudo
     CDS             14468..15578
                     /locus_tag="WM704_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            15587..16414
                     /gene="fghA"
                     /locus_tag="WM704_000555"
     CDS             15587..16414
                     /gene="fghA"
                     /locus_tag="WM704_000555"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFA9340041.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNRENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            16944..18095
                     /locus_tag="WM704_000556"
     CDS             16944..18095
                     /locus_tag="WM704_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HFA9340042.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            18154..18579
                     /gene="ndk"
                     /locus_tag="WM704_000557"
     CDS             18154..18579
                     /gene="ndk"
                     /locus_tag="WM704_000557"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFA9340043.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            18723..19817
                     /gene="rlmN"
                     /locus_tag="WM704_000558"
     CDS             18723..19817
                     /gene="rlmN"
                     /locus_tag="WM704_000558"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFA9340044.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            19820..20581
                     /gene="pilW"
                     /locus_tag="WM704_000559"
     CDS             19820..20581
                     /gene="pilW"
                     /locus_tag="WM704_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFA9340045.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            20597..21862
                     /gene="ispG"
                     /locus_tag="WM704_000560"
                     /gene_synonym="gcpE"
     CDS             20597..21862
                     /gene="ispG"
                     /locus_tag="WM704_000560"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFA9340046.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(21916..22530)
                     /gene="clpP"
                     /locus_tag="WM704_000561"
     CDS             complement(21916..22530)
                     /gene="clpP"
                     /locus_tag="WM704_000561"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFA9340047.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(22626..23936)
                     /locus_tag="WM704_000562"
     CDS             complement(22626..23936)
                     /locus_tag="WM704_000562"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFA9340048.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         6254 a         6441 c         5626 g         5804 t
ORIGIN      
        1 tttccttttc tggaaaataa caacttagcc tcatatagta gtaggcacta atttaagaaa
       61 atccttgaag cctcctacta tctagaagcc ttggatatat ttcaaaaatt aacctgtttt
      121 aaatcttaca agccccacca gcgcagccgt catcttgaat atcggtctgc gtgtcgtccg
      181 cgccgtcgcg ggtgttgtgg tagtacaggg ttttgacgcc gtatttgtag gcagtcagca
      241 ggtctttgag catttgtttc atagaaactt tgttaccttc gaatttgccc gggtcgtaag
      301 cagtgttggc ggaaatcgcc tgatcgacga atttttgcat tacgccgaca agtttcaggt
      361 agccttcgtt gcccggaagc tgccacaggg tttcgtaggc atttttcaag gtttcaaact
      421 ccggcacgac ttgtttcaaa atgccgtctt tcgatgcttt gaccgttacc aatccgcgcg
      481 gcggctcgat gccgttggtt gcgttggcga tttgcgagct ggtttcagac ggcatgagcg
      541 cggtcagagt agagttgcgc aggccgtatt tgacgatgtc ggcacgcagg ctttcccagt
      601 cgtaatgcaa aggctcgccg cagacggcat ccaaatcttt tttgtaggtg tcgatgggca
      661 gtttgccttg cgaataaacg gtttggttaa agagcgtgca cgctccgtat tctttggcaa
      721 ggtttgccga tgctttgagc aggtaatact gtatggcttc aaaggtacgg tgggtcagac
      781 cgagcgcgga accgtcgctg tagcggacac cgtttttcgc cagatagtag gcgtagttga
      841 tgacaccgat gccgagcgaa cgccggtcca tagtagaggt acgcgcggct tctaccggat
      901 atccctgata atctaaaagt gcatcgagtg cgcgcacggt caaatcggca agcccttcca
      961 attcgtccaa gctgtttaat gcacccaagt tgaaggcgga cagtgtacac agggctattt
     1021 cgccgtcagg gtcgttgatg ttgtccagcg gtttggtcgg cagggcgatt tccatacaca
     1081 agttggactg atgaacaggc gcaacgcgcg gatcgaacgg gctgtgcgta ttgcagtgat
     1141 cgacgttttg aatgtagatg cgcccggtcc cggcacgctc ctgcatcagc gtggaaaaca
     1201 ggtcggcagc cggaatgata cgcttgcgta tgttcgggtc ttgctcgtat ttcgtataga
     1261 gccgctcaaa ttcgtcttgg tcggcaaaaa acgcttcgta caaccccgaa acctcgttgg
     1321 gcgaaaacag cgtaatgttg ccgcccttaa tcaggcgggt gtacagcagg cggttgattt
     1381 gcacgccata atcaagctgg cggatacggt tgtcttccac gccccggttg tttttcaaca
     1441 ccagcaggct ttcggcttca atatgccaca aggggtagaa caaggttgcc gcgccgccgc
     1501 gcacgccacc ttgcgaacaa gatttgaccg ccgcctgaaa catcttaaag aagggaatgc
     1561 agccggtatg ccgggcttca ccgccccgga tttcgctgtc cagcccgcgg atacgtccgg
     1621 cattgatgcc gatgcccgcg cgttgggaaa cgtatttcac aatcgcgctg gtagtggcat
     1681 tgatggaatc caaactatcg tcgcattcaa tcagcacaca gcttgagaac tggcgcgtag
     1741 gcgtacgcac gccgctcata atcggcgtgg gcaacgatac tttgaatgtg gaaacggcat
     1801 cgtaaaaccg tttgacgtaa cccaagcgcg cctctttcgg gtatttgctg aaaaggcaca
     1861 tcgccaccaa aacatataaa aactgcggcg tttcgtaaat ctggcgggta acgcggttct
     1921 gtaccagata tttgccttcg agctgtttga cggcggcata ggaaaaggac atatcgcgtt
     1981 cgtggtcgat ataggcgttc agttcgtcaa attcttcgcg gctgtaatcc tcaaggatat
     2041 gcctgtcgta ttttccggca tcggtgatct ttttaacatg gttgtaaagg tgcggcggct
     2101 cgtattcgcc gtaggctatt ttacgaagat ggaaaatcgc caaacgtgcg gcaaggtatt
     2161 ggtagtccgg ggtatcttcc gaaattaaat ctgcagcggc tttgatgatg gtttcgtgga
     2221 tgtcgtcggt gcggatgccg ttgtagaact ggatgtgcga tttcaactcg acctgcgaca
     2281 cggaaacatt ttccaatcct tccgccgccc aagtaacgac acggtgaatc ttatccaaat
     2341 caatggcttc caatcttccg tctcgtttgg ttacttttaa atcagtcggt gtattcatcg
     2401 cttcctcttc cactcttgat attcaagaca cggtcttttc aaataaatta aggcagacaa
     2461 tatagtggat ttttagcatt ttctccagtc ttgacaacga ttgcattttt cagtttcagg
     2521 caatgcgcga taaaacccgc ctgtcgtatt tttcctataa catttgtttt atctgataat
     2581 tctttaccga taaaaaacgg gtaaattttt tgccttttga ccggctccgg ctacaaggcg
     2641 atgaaataag gattttccga cgaaaaagga aaacttccgg ttttccgccc ctgcgaattg
     2701 ttaaattttg caaagtatga tttttgccac gccgccgccg acaaattcca ttttcttacc
     2761 gattggaatt tattattgag attaatatgt tatttgaatt tgcatatcaa acggcaagtt
     2821 ttgtcggctg aatgagtctg aaactgccga cagtttgccc gttccatcct tccatcctat
     2881 actggaagga attacaaact gaaaggatcg tcatgtccgt caataaagat gcttgccata
     2941 ctgttgtctg caatactggg actggtatca acaactgccg ctgccggtac gtcagaaccc
     3001 gcccaccgac ataccaaaca tatcagcaag gcaaacaagc agatgctgca ccccgaatgc
     3061 aggaaatatt tggaacgccg tgccgcgtgg taccgatcgc aaggcaacgt gcaggaattg
     3121 cgcgaaaaca aaaaggcgcg caaagcattc cgcaccctgc cttatgcgga acagaaaatc
     3181 caatgccggg cggcttatga ggctttcgat gatttcgacg gcggcaggtt ccgccgttaa
     3241 tcccatataa aatatgctgt ctgaacacaa gttaagatgg cattttccat agatatagtg
     3301 gattaaattt agaccggtac ggcgttgcct cgccttgccg tactattttt actgtctgcg
     3361 gcttcgccgc cttgtcctga tttaaattta atccactata caacaaacta tccttacggc
     3421 atttcttttg aggtactccc gttaccgcag ccttaaggca tccgcaaaag cgaatcatac
     3481 cgctacattc cgacaaccgc tttcagacgg caacatccgc atcgcctgcg acaacggcac
     3541 ggatgctttc ttcgatttta tcttttaaag cataacggtc ttcgctttcc gccgcgtccg
     3601 ccacgcaaac gaaatcgact ttgatagtca gttttttcat agacacgatt cgccacaggc
     3661 aagtcggcaa accgacatcg gcatacgacg gacgggccgt cctttttccc gtttcgtcat
     3721 aataacgcag tgcgaccgcc aaaacctttg cccccgcatc gatggcggat tggaacagcg
     3781 cagccttgaa cggcaggagc cccagtccgg aggaagttcg cgcctcaggg aaaaaactga
     3841 cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt aatcggttcg atgtcgcgcc
     3901 gcgaattgcg gttgatgaac accgttcccg cgttctgccc catcttgccc aataccggcc
     3961 agcttttgat ttcctgcttg gcgataaagc tactcggata aaccgcgctc atcgcgaaaa
     4021 tatccagcca ggacacgtgg ttggcggcaa ccaagacacc gttcggatgt tcgggtgcgg
     4081 gtctgcccac ctccaatccg atatccaaag ccgccaaagc gcccttgccc aacgctataa
     4141 ctgcccgatt gcgcgacttg gggcagccgc cgtcaatacc gcgcaggttt ttgccggttt
     4201 tgaacagcca gaccgtcaag cggcacaagc ggcgcagacg tgtaaaaaat gaagctttat
     4261 ttgaagacat gggcaattct ttcccgataa tcggtgtttg ttgttgaaac gttcggacgg
     4321 cataccgata aaatgccgtc agaaaccgtt ttccgcccta tttctgacag ttcgtgcaat
     4381 aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa accgccgcac cggaggcacg
     4441 gctgattgtg ccgcccgtac accgtatatt cctgttgaaa gtagccgctt ttgccgtcgc
     4501 tgtccacaaa atccctcagc gtactgccgc ccgtttcaat ggcgcgctgc aacaccgctt
     4561 tgacggtttc aaccaaaacc gcgcactctt tcttttttag gcggttggca ggacggtggg
     4621 gcgaaatgcc cgccctgaac agactctcgt tggcataaat attgcccacg ccgaccacga
     4681 cggtattgtc catcagagcg agtttgaccg cgcgtttctg cgccttcagc cccgcataca
     4741 gataatccgt gcaaaatgcc tccgacaaag gctccggccc cagtttttcc aacagcggat
     4801 gacgttcttc gattccctca taccaaagta tcgcgccgaa ctttctcgga tcgcggtaac
     4861 gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc gtgtctgtcc ggcctgccga
     4921 tacgtccgtc cgacggcgta aagatccgca agctgcccga catccccaag tgaatcagca
     4981 aaatccccgt ttggaaacgg acaatcagat atttggcacg cctgccgcag gacaaaacct
     5041 gccggccgga caaaatctcc cccaaatcgg gattgatctg ccagcgcagc ttcagttggc
     5101 gcaatatcac ggcttccacc gtttttcctt caatatgcgg cgcgatgccg cgcaacgtcg
     5161 tttccacttc cggcaattcc ggcataaccc ctcccgacat ttcttctgac agatgccgtc
     5221 tgaaagacgg ccgtccctta atccgcaacc cttgccgcac ccgccgcaag ggctttgccg
     5281 ccccaatacc cgtcccacgc gggcaggggc gttacgcccg ccgccctttt gaccaaaacc
     5341 aaaccgtaca ctgcggcaca ttgcggccac caacggtcgc ccgccttttc cataaaccgc
     5401 caaaagcgta tttgcccgag cgacgaaacg ggaggcagat acaccatgaa tttcccaaat
     5461 tcaatatcga aaccggcatc ggcaagctgc cttttcaact cgggcagcgg cagacaaaac
     5521 cgtttttccg gcagacggac accgtcaaac caacggcaga atccccaaag cgaatacgga
     5581 ttgaaacccg tcagcatcaa acgtccggac ggtttcaata tccggtgcgc ttccgacagg
     5641 atttgcgaag gtacgccgca ttccagcgta tgcggcaaaa gcagcatatc cgcaaaaaca
     5701 tccgccaaag ccatattctc cgccgacatc gacatatctc gcggcacaca gacaatgtct
     5761 tcagacaggc tcagccactg accgcccacc tgaaccgcac acattcccga aaaacggtat
     5821 gacgccacat accgattaaa gaagtcctgt tccaattttg caacataccg ccccatcgcc
     5881 gtatcttcaa accatgcatc catattgcgt ccgtttcaaa cagattgcct gccgatattt
     5941 tattccaaac aggatttctg tcaaaaaaca catcggccgc ccatttccga atccgcataa
     6001 agttcctgta aaacttgacg ctttttcagt caaacagtac catcggacga taaaatatgt
     6061 ttattccgcc gagtataaat catgtccaaa ctcaaaacca tcgccctgac cgcatcaggt
     6121 ctgtccgttt gtccgggttt cctatatgcc caaaacacct catcacacca agtcggttta
     6181 gcgattatgc ggttaaactc ttcaatactc gacctgccac cgacaaaaca atatttccaa
     6241 tccggcagcc tgtgggacga gctgcgccaa ggcttccgga tgggcgaagt caatcccgaa
     6301 ctggtacgcc gccacgaaag caaattcatc gcaagccgca gctatttcga cagggtcgtc
     6361 aaccggagcc gaccctatat gtaccatatc gccaacgaag tcaaaaaacg caatatgccc
     6421 gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg tcaccaaagc caaatcacac
     6481 gtcggcgcat cgggcctgtg gcagttcatg cccgctaccg gcaggcatta cggcttggaa
     6541 aaaacaccgg tttacgacgg caggcacgac gtttacgcag ccaccgatgc cgcactcaac
     6601 tatctgcaat atctctatgg actgttcggc gactggccgc tcgcctttgc cgcctacaac
     6661 tggggtgaag gcaacgtcgg acgcgccgtc aaccgcgccc gcgaccaagg gctcgaaccg
     6721 acctacgaaa acctgcgtat gcccaacgaa acgcgcaact atgtccccaa gctgctcgcc
     6781 gtgcgcaaca ttattgccac cccccaatct ttcggcatga atatcagcga catagacaac
     6841 aaaccctatt ttcaggcagt cgaaccgggc cgtccgctcg acaacgaagc catcgcccgg
     6901 cttgccggca tcacgcaaag cgagctgctc gccctgaatc ctgcattcaa cgtccccgcg
     6961 ttcatcccca aaaacaaacg caaactgctg cttcctgtcg cgtccgtcca aaccttccaa
     7021 agcaactacc tcaacgccgc acccgacagc ctgttttcat gggaagtcta tacgcctgcc
     7081 gccaaaacca gcctgtccga catctcgacg gcaaccggca tgagcattgc cgacatcaaa
     7141 cgcctcaaca acctgaacgg caaccttgtc aacgcaggac gcagcatcct tgtcgccaag
     7201 aacggcaaga cccttcatac ggcatcggaa tccgtcgttt ccatcgacat cgacaatacg
     7261 cccgacacct accgttccaa tatgccggca ggcacggtga acgtcagcat tgcccgaatc
     7321 caacccgccg ccgcacagac agcggacatt accgtcgcac ctttgccgca agaaaccgtc
     7381 cgtacggaac ccgatcccct tgtccgcatt gccgaacccg cccttgcgac agccgcagcg
     7441 caacctcaaa ccgaaaaaca gactgccatg ccgtctgaaa cccaaaccgc aacactcgcg
     7501 caggtcgtcc ccccaaacga catgcaggcg gcagacgaac tcatgcagct tgttgcccga
     7561 aacaacctgc gccggcaggc tgaagaaacc atctccgccg tcatcggcac gcctgacaca
     7621 gttgccgaac acaacatttc ctcatctccg caacataccg ttgccgccga cggcaaacgc
     7681 cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg gcgaagccga aacctccccg
     7741 ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc tgttcaacat tgccaaacgc
     7801 tacaacgtca gcgtagccga cctgattgtc gccaacaaca tcaaaggcaa caccatccaa
     7861 aaaggacagg tactccgcct ggcgcaggca gcccctgccc aaacccgtat tgaaaaagta
     7921 tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg ccgcgcgctt caatatccat
     7981 atcgacgaca tccgccggct caatcccaac ctgaacacca tcaatccggg acagagggtc
     8041 aaactgattg gaagctgatt cggatacggc acatgacagg actttctcag tcctgtcttt
     8101 ttcatcccac atttcccata ccatcatgaa actcatcaaa tacctgcaat atcaaggcat
     8161 aggaagccgc aagcagtgcc aatggctgat taggggcggc tacgtctcca tcaacggaac
     8221 ctgcatggac gacaccgatg cagacatcga ttcctcatac gtcgaaacgt tggatattga
     8281 cggggaagca gtaaccgtcg ttcccgaacc ctatttctac atcctgctca acaagcctga
     8341 agattacgaa acttcgcaca aaccgaaaca ctaccgcagc gtattcagtc tgttccccga
     8401 caatatgcgg aacatcgata tgcaggcggt cggcaggctg gatgcggata cgaccggcgt
     8461 attgctaatt accaacgacg gcaaactgaa ccacaacctg acttcgccga gcagaaaaat
     8521 tcccaagcta tacgaagtaa cgctcaaaca ccctacagga gaaacgctct gcgaaacttt
     8581 gaaaaacggc gtgccgctcc acgacgaaaa cgaaaccgtt tgtgccgccg atgccgtttt
     8641 ggaaaatccg accaccctgc tgctgaccat taccgaagga aaataccacc aagtcaaacg
     8701 catggttgcc gccgccggca accgcgtgca acaccttcac cgccggcgat tcgcacattt
     8761 ggaaacagaa aacctcaaac ccggggaatg gaaatttatc gaatgtccga aattctgaaa
     8821 caaaattcca taatttccct atattcaacc atatgcttat taaataagca gttttacaaa
     8881 aattaactca aaaaaacagt tagtttacaa tataatccgc ttgtctaagt aacgcctgtt
     8941 caagcaggat tatgagcaag acgttttccc cgtaatgtgt tttgccatct atacttcccc
     9001 ccttgtatag atggttttct atttcaaatt ccctctcttt catcatcatt gaaatgctat
     9061 cattaatatg attaacaaaa tttcacttcc gtacaccgat gtagaaaaat aacagattaa
     9121 tctgttcata aactccgatt cttcctcatc ggtttatact catcagaaat attcactgaa
     9181 atatatgcat tttacatgga aaatcactag gcagttgaaa aacattcagt caatttctgt
     9241 taagaatcac gtgtaaaatt tccgctttat ctcaatcgga cacggtttca atatgtcttc
     9301 cccttcaaat accaaccgtc aaacctggtc cagccgtttg acttatatcc ttaccgttgc
     9361 cggcgcgact gtcggtttcg gcgcgacgtg gcgtttcccg tatttggtcg gtgaaaacgg
     9421 cggcggcgcg tatgtgtttt tattctgtat cgcgatgctg gttatcggca tcccgatgat
     9481 tttggtggaa aacgtcatcg gacggcgcaa aggcgtgaac gcgctggatg cgttcggcgg
     9541 cccgatgaac ggcaaacctg ttgccaaaat ttggaaactg gtcggccgga tggggctgct
     9601 cggcgcgttc ggcatcatgg cttattatat ggtactcggt ggctgggtaa tcagctatat
     9661 cgttaatatt attggaggaa atttgaatat ttccagcccc gtcgacggtg tggttacaaa
     9721 aggcttcttt accgaacaca ttgaaaacag cccttgggaa attgcgtttt atacgctgct
     9781 ttttgtcgcc gtgaaccaat ggattttggt caaaggcgtt atcggcggca ttgaaaaaac
     9841 ggcgaaatac ctgatgcctc tgctgttttt gttcctaatc gcgatggtcg tccgcaacgt
     9901 tacccttccg ggcgcaatgg aaggggttgc tttctatctg aaacctgatt tcagcaagat
     9961 taccgccgaa ctgttcgtct tcgttttggg acaggtattt tttgccctga gcctgggttt
    10021 cggcgtgatg attaccttat ccagctatct ggataaaaac gaaaatctgg ttcagacggc
    10081 agttatcacg gcaattacca ataccatcat cgctgtactt gcgggcttta tgattttccc
    10141 gtcgctcttc agcttcggcg tcgcgcccaa ttccggcccg actttggtgt tccaaagttt
    10201 accgattgtg ttctcacata tgtgggcagg acctgtgttc gccgtcattt tcttctctct
    10261 gctcctgatt gccgcgttga caacttcgct gacgatttat gaagtgttga ttacggccat
    10321 tcaggaaaaa accaaaatcc gccgtaccgc cgcaatcacg attgtattgt ccgtcatctt
    10381 cgttttcggc aacatcccgt ctattttgag ctatggcccg tggaaagacg tttccgtgtt
    10441 cggcaaaaat attttcgatg ccttcgacta catcagcggc aacatcttgt ttatgctgac
    10501 cgcgctcggt tccgcattgt ttgtcggttt tgtgatgaag gacgaagcga aggaagaatt
    10561 gctttataaa ggcaaccata cgacggtcaa tatttggttt gcctatgtga aataccttgt
    10621 gccgctggtg attctgctga ttttcgtcag caacctgttc taatccgcag caatcaatgc
    10681 cgtctgaagg tcatacctct ttcagacggc attgcattga tgcccgccac gccggaatcc
    10741 gaaccggctt gccggcttct gtcggacgct ttccgggcag gcggcgttcc gtctgcttag
    10801 acaaccgtcc tttaaaacag gtagaatccg ccccaacggg aaacacaccc ttcagacggc
    10861 aaaacccata ccccaaacca tcaggaatcc cccttatgaa cagacaaaaa gtcatcgcca
    10921 tcgacggtcc gggcgcatcg ggcaaaggca cggtcgccgc ccgcgttgcc gccgcattgg
    10981 gatacgatta tctcgatacc ggcgcgctct accgcctgac cgccctatat gcacaaaaac
    11041 aaggcgtgga atggcacgat gaagaaaacg tttccgcact tgccaaaaaa ctgcccgccg
    11101 tattttcagg caaccgcatc ctacttgatg gcgaagacgt ttcagacggc atccggacag
    11161 aagccatcgg tatgggcgca tccgcagttg cgcaatggcc caaagtccgc gccgcactgc
    11221 tgcaacgcca acgcgatttt ctgaccgaaa aaggactggt tgccgacgga cgggacaccg
    11281 gatcggtcgt cttcccccaa gccgaactca aaatttttct gacagccgaa tctaaaatcc
    11341 gtgccgaacg ccgcgccaaa caaatcggca ttccctgcga aggtttcaca ttcgagcgca
    11401 tcctgtccga catcgagacc agagacgaag cagaccgaaa ccgcaaggtt gcccccctga
    11461 aacaacagcc cgatgccctg cttttggata cgagccgcct aactatagaa gaaactgtaa
    11521 aaaaagtgct tgattggtat cgtaaagttt aaattttcag gtataatcgc ccgcattgcg
    11581 cttcagacag catgaatttc catgtgccgt ctgaaacctt atttacccgc ctgccgtgcc
    11641 aaggactgca gacacccacc aacccaaccc gcaccccttg gcggtgtacc gaaaagagtt
    11701 atatatgtct atggaaaatt ttgctcagct gttggaagaa agctttaccc tgcaagaaat
    11761 gaacccgggt gaggtgatta ccgctgaagt agtggcaatc gaccaaaact tcgttaccgt
    11821 aaacgcaggt ctgaaatcag aatccctgat cgatgtagct gaattcaaaa acgctcaagg
    11881 cgaaattgaa gttaaagtcg gcgacttcgt taccgttacc atcgaatccg tcgaaaacgg
    11941 cttcggcgaa accaaactgt cccgcgaaaa agccaaacgc gcagccgatt ggatcgcttt
    12001 ggaagaagcc atggaaaacg gcaacatcct gtccggcatc atcaacggta aagtcaaagg
    12061 cggcctgacc gttatgatca gcagcatccg cgcattcctg ccgggttctt tggtcgacgt
    12121 acgtcccgtt aaagacactt cccattttga aggcaaagag atcgaattca aagtgatcaa
    12181 actggacaaa aaacgcaaca acgtcgttgt ttcccgccgc gccgttttgg aagccacttt
    12241 gggtgaagaa cgcaaagccc tgctggaaaa cctgcaagaa ggctccgtca tcaaaggcat
    12301 cgtcaaaaat atcaccgact acggcgcatt cgttgacctg ggcggcatcg acggcctgct
    12361 gcacatcacc gatttggcat ggcgtcgcgt gaaacacccg agcgaagtct tggaagtcgg
    12421 tcaggaagtt gaagccaaag tattgaaatt cgaccaagaa aaacaacgtg tttccttggg
    12481 tatgaaacaa ctgggcgaag atccttggag cggtctgacc cgccgttatc cgcaagccac
    12541 ccgcctgttc ggcaaagtat ccaacctgac cgactacggc gcattcgtcg aaatcgaaca
    12601 aggcatcgaa ggtttggtac acgtctccga aatggactgg accaacaaaa acgtacaccc
    12661 gagcaaagtc gtacaactgg gtgacgaagt cgaagtcatg attttggaaa tcgacgaagg
    12721 ccgccgccgt atctctttgg gtatgaaaca atgccaagcc aatccttggg aagaatttgc
    12781 cgccaaccac aacaaaggag acaaaatctc cggtgcggtt aaatccatta ccgatttcgg
    12841 cgtattcgtc ggcctgcccg gcggcatcga cggtctggtt cacctgtccg acctgtcttg
    12901 gaccgaatcc ggcgaagaag ccgtacgcaa atacaaaaaa ggagaagaag tcgaagccgt
    12961 cgtattggca atcgatgtgg aaaaagaacg catctccttg ggtatcaaac aactggaagg
    13021 cgatcctttc ggcaacttca tcagcgtgaa cgacaaaggt tctttggtta aaggttccgt
    13081 gaaatctgtt gatgccaaag gtgctgttgt tgccctgtct gacgaagtag aaggctacct
    13141 gcctgcttcc gaatttgcag ccgaccgcgt tgaagacttg accaccaaac tgaaagaagg
    13201 cgatgaagtt gaagccgtca tcgttaccgt tgaccgcaaa aaccgcagca tcaaactttc
    13261 cgttaaagcc aaagatgcca aagaaagccg cgaagcactg aactccgtca atgccgccgc
    13321 caatgcgaat gccggtacca ccagcttggg cgacctgctg aaagccaaac tctccggcga
    13381 acaagaataa ggttgcagac atgacaaagt ctgagttaat ggttcgcttg gcagaagtat
    13441 ttgccgccaa aaacggcacg catcttctgg caaaagacgt agagtacagc gtaaaagtct
    13501 tggttgacac catgacccga tcgcttgccc gaggtcaacg catcgaaatc cgcggtttcg
    13561 gcagcttcga tttgaaccat cgtcctgccc gcatcggtcg caatcccaaa accggcgagc
    13621 gcgtggaagt acctgaaaaa catgtacccc acttcaagcc tggtaaagaa ttgcgcgagc
    13681 gggtcgactt ggctttaaaa gaaaatgcca attaaacctt agcatcaaaa cgccgctggt
    13741 acgcggcgtt ttttctttgg tttaacttca tccgctgctt caatacctta agccaagcaa
    13801 gcaacggatt agagcgtgga ttttttacca ttgcttacaa tccgcttttt aaacaacaaa
    13861 ttgttgattt ctattacgaa caggacaaaa tcctgcttat tgcactaaaa ctaagccgtt
    13921 tcaggaattt gcggcaaatt tacagctttt accgagccta atgctttcgc tttttggtaa
    13981 aacgccaatt tgtattcaag caaatctaaa taacgtttta attcggcaat ttgacacttc
    14041 acattttcta tttgattttc aaacaaggaa aggcgttctt caatggtatc gtcgccaatg
    14101 acggtacatt ccgcaaagcg tttgatgtct tttaagctca ttcccgtatt tttcaagcat
    14161 tgcaataagt acaaccattg caaatcgtta tcggtaaaac aacggttacc gtattcatca
    14221 cgtccgatat taggcaacaa gccttctttg tcgtaaaagc gtaaagtgtg ggcggagatg
    14281 cctatttttt cggcagcttt ggcagtggta taagtcattt tccctccctc taaacaaaaa
    14341 acggtaaaaa aacacttgcc ttagagtgaa ctctaaaatg taaactgttg ctatgttgct
    14401 caggcaaggc tattttgtta atgaattaag aggaaagaca atggaaatga aacaaaccga
    14461 ttcaaccatc aaatctcgtg cggcggtggc attcgcccct aaccaaccct tacaaattgt
    14521 ggaaatcgac gtggaaatgc cgcgtaaaga cgaggtgtta attcgtaaca cccacactgg
    14581 cgtgtgccat actgatgcat ttaccttatc aggaagcgat cctgaaggcg tattccctgt
    14641 ggtgcttgga cacgaaggtg cgggtgtggt cgttgctgtg ggcgagggtg tgtcaagcgt
    14701 aaaaccgggt aatcacgtca ttccgcttta caccgcagaa tgtggcaaat gtgagttttg
    14761 cctctcgggt aaaaccaatt tatgcgtctc agtgcgtgat acacaaggta aaggcttaat
    14821 gccagacggc acgacgcgtt tttcttatca aggtcagccg atttatcact atatgggctg
    14881 ttcgactttc agtgaatatt ctgttgttgc cgaagtttca ctggcgaaaa tcaacccgaa
    14941 agccaaccac gaacaagtat gtttgctcgg ctgcggcgtt accactggta ttggtgcggt
    15001 acataacacg gcaaaagtac aagaaggcga ttctgttgcc gtgtttggct tgggagcgat
    15061 tggtttggct gtggtgcaag gtgcgcgtca agccaaagcc ggtcgcatta tcgccattga
    15121 taccaatcct gcaaaattcg agttggcaaa acagtgttgg tgcaactgac tgtttgagcc
    15181 cgaaagatta cgataaacca atcaaagatg tgttgttaga catcaacaaa tggggcattg
    15241 accacacctt tgaatgtatc ggcaatgtaa acgtaatgcg tcaggcatta gaaagtgcac
    15301 accgaggttg gggacaatcc atcatcatcg gcgtagcgga tgcaggacaa gaaatttcaa
    15361 cgcgtccgtt ccaatcggta acaggccgtg tttggaaagg ctcggcattt ggtggtgtta
    15421 aaggccgctc cgagcttccg aaaatggtgg aggattcaat gaaaggcgat atccaattag
    15481 aaccatttgt gacccatgca atgacccttg atcaaatcaa tgaagccttt gagttaatgc
    15541 acgaaggtaa atcgatccgc gctgttattc attactaagg tccacgatga aactgattga
    15601 acaacatcaa atttttggtg gttcgcaaca agtttgggcg catcatgccc aaacgcttca
    15661 atgcgaaatg aaatttgccg tctatttgcc ggataatcgg gaaaatcaac cgcttggtgt
    15721 gatttattgg ctttccggct tgacgtgtac ggaacaaaat ttcattacca aatcgagctt
    15781 ccggcgttat gcggcagaac atcaagtggt tgtggtcgcc cccgataccg gccctcgcgg
    15841 agagcaagtg ccgaacggtg ccgcttatga tttaggacag ggggcaggct tttatttgaa
    15901 tgcgaccgaa cagccttggg cggcgaatta tcaaatgtat gattacattt tgaacgagtt
    15961 gccccgtctg attgaggagc attttcctac caacggcaaa cgttccatta tggggcattc
    16021 gatggacgga cacggcgcat tggtattggc actacggaat cgggaacatt atcaaagtgt
    16081 ttctgccttt tcgcctattt tatcgccaag cctcgtaccg tggggagaaa aagcctttac
    16141 tgcttattta gggaaagacc gtgaaaaatg gcagcaatat gatgctaact cactcattca
    16201 acaaggctat aaagtgcaag gtatgcgtat cgatcagggc ttggaagatg agtttttgcc
    16261 gacacaattg cgtaccaaag attttatcga aacctgtcgt gcggcaaatc agccaatcga
    16321 tgtgcgtttc cataaaggat acgatcacag ctattacttc atcgccagtt ttattggtga
    16381 gcatatcgct tatcacgccg catttttgaa gtaagtcaaa gagcggtcag ttttcaaagc
    16441 agtttggaat agccggcacg agggcggtaa gaagtgccgg cataaacgta tgccgcctga
    16501 accagaagga gccggcccta cggattacca aaccctgccc ttgatttgat tagtatgatt
    16561 tttaagctta aaccctgatt gatcaaacat taaaatcgcc cgtatttcct acttcacttt
    16621 cgggcttcgt attattcgtt gccgaatttg cataagcaac ctgttcctgt tgtgttttcg
    16681 ccccctccaa gctgacggtg gcgtaacagc ttcgcgattc accatctgtt tcatttgtcc
    16741 tccttcaata ttaaaacgca gcctgaaata aaaggcattt tggctcatat ctgcaccata
    16801 ttaaaacgcc gcctttgctt ataccccctt tgtgcgcgtc attattcttt tccacgggaa
    16861 acgccaagtt tgaaggaaat catttataat accagcggta agcattttct ttcttagccg
    16921 caagaagtat aacaaggtta aatatgaata atagagacca actttttaaa gccccgccgt
    16981 ttgaaaacca cagcccgctg acctggtatc aggctgcctc acaactgccc aacttcatcc
    17041 gcgacgacgc acaggcggcc gccatcgaac accttgaccg gctttggacc gaattgatga
    17101 tgttcaaacg caaaagaaac cgttttttag gcaggagttt gcgttccccg caagttccca
    17161 aagggcttta tttctatggc ggagtcggac gcggcaagag ctttctgatg gacgcttttt
    17221 tcggctgcct cccttaccgc cgcaaacgcc gcgtccactt tcatgccttt atggcggaaa
    17281 tccaccggcg gctgaaagcc ctgaaaagcg aaagcaaccc gttgaaatcc gttgccgccg
    17341 agattaccaa agaaacccga gtattgtgtt ttgacgaatt tcatgtcagc gatattgcgg
    17401 atgcaatgat tttaggccgt ctgctggaaa acctgcttaa cgagggcgtt gttttggtgg
    17461 cgacttcaaa ctacgcgcct tccgaactct acccgcaagg tcaaaaccgg agcggttttc
    17521 ttcccacaat cgcgctcatc gagtccagtc tgaccgtctt aaacgttgac ggaggggaag
    17581 actaccggct gcgtaccctc cgccccgccg agattttctt tacgcctgcc aatgaagaaa
    17641 atgaggcaaa actggcaaaa ctgttcaaag aaatgacagg cattaccgat ttgaaccccg
    17701 gcatcagcac catccacggc cgggaaattc cccacaaagc cgagtccggt cgtaccatat
    17761 ggtttgattt ccgcgcactg tgcttcagtc cccgctcaca gtccgactat ctgtatttgg
    17821 ccgaacatta tgaaatggtt tttatttcag gtttggaaca actctcaccg caagaaaagg
    17881 cggaggcgcg acggctgact tggctgattg acgtactcta cgatttccgg gtcaaactgt
    17941 gtgccaccgg cgcggtagat gtcaaccata tctacacgga aggcgatttt gccgaagaat
    18001 ttacccgcac cgccagccgg atggtcgaaa tgcagtccga agtttatttg gaacagccgc
    18061 acctgacctt atctcccaag gcttcaggcg gataagttat ttttttggta gaataccgat
    18121 ttgattcttc ttaagtaaaa ataaggatat agcatggcga ttgaacgtac catatccatc
    18181 atcaaacccg atgccgtcgg caaaaacgtt atcggcaaaa tatacagccg ctttgaggag
    18241 aacggtctga aaatcgttgc cgccaaaatg aagcagctta ccctcaaaga agcccaagaa
    18301 ttttatgcgg ttcataaaga ccgccccttc tatgccggat tggttgaatt tatgaccggc
    18361 ggtccggtta tgattcaagt actggaaggc gaaaacgccg ttctgaaaaa ccgcgaactg
    18421 atgggggcga ccaatcccac cgaagccgca gaaggcacga tacgcgcgga ctttgccact
    18481 tcggtcagca ttaacgccgt acacggttcc gacagtgtgg aaaatgccgc tttggaaatt
    18541 gcctactttt tcagccaaac cgaaatctgc ccccgttgat acaatacacc gcccaattcc
    18601 tattcagacg gcataaaata tatccatgcc gtctgaaaac tctgttgcaa aagacttcaa
    18661 atcaaacttg cctgccctgc aattttttat ttgaagcctt aatttaagaa aaacacaaat
    18721 acatgaaaac caatctgctc aactacgacc ttcaagggct aacccgacat tttgccgata
    18781 tgggtgaaaa accattccgc gccaaacagg ttatgcgttg gatgcaccaa tccggcgcgc
    18841 aaaattttga cgaaatgacc gatttggcaa aatcgttgcg ccataaactg aacgaacagg
    18901 caagcatcga aattcccaag ctgatgatgt cccaagaatc ttcagacggc actcgaaaat
    18961 ggcttttgga tgtcggtacg gggaacggcg tggaaaccgt cttcatcccc gaatcggatc
    19021 gcggcacgct ctgcatttcc tcgcaagtcg gctgcgcttt ggaatgtaca ttttgttcga
    19081 ccggccggca gggcttcaac cgcaatttaa ccgctgccga aatcatcgga cagttgtggt
    19141 gggcaaacaa agcgatgggc gttacaccga aaaacgagcg tgtgatttcc aacgtcgtca
    19201 tgatgggcat gggcgagccg atggcgaact tcgacaatgt cgttaccgcc ttgagcatca
    19261 tgctggacga ccacggctac ggtttgagcc gccgccgcgt aaccgtttcc acttcgggta
    19321 tggttcccca aatggacagg ttgcgcgatg tcatgccggt ggccttggcg gtttccctcc
    19381 acgcttccaa tgacgaagtc cgcaaccaaa tcgtaccgtt gaacaaaaaa tatcccttga
    19441 aagaattgat ggccgcatgc caacgctatc tggtcaaagc gcccagggat ttcatcactt
    19501 tcgagtatgt gatgctggac ggtgtcaacg ataaggcaca acatgcatac gaacttattg
    19561 aactggtcaa agatgttccc tgcaaattca atttaatccc gtttaatcct ttccctaatt
    19621 cgggatatga acgttcaagt aacgaaaaca tccgtatttt cagagatatc ctgcaacaag
    19681 ccgaatttgt cgttaccgtt cgaaaaactc gtggcgacga tatcgatgcc gcctgcggac
    19741 agttggcagg tcaggttcag gataaaacgc gccgccaaca aaaatggcag cagattttaa
    19801 tcggacaaca ggggtaatta tgccttttaa gccatccaaa cgaatctctt tattacttgt
    19861 tcttgccttg ggcgcgtgca gcacttccta ccgcccctcg cgggcagaaa aagccaatca
    19921 ggtttccaat atcaaaaccc agttggcgat ggaatatatg cgcggtcagg actaccgtca
    19981 ggcaacggca agtattgaag atgccttgaa atcgaaccct aaaaacgagc ttgcctggct
    20041 ggtccgtgcc gaaatctatc aatacctgaa agttaacgac aaggcgcagg aaagtttccg
    20101 gcaagccctc tccatcaaac ccgacagtgc cgaaatcaac aacaactacg gctggttcct
    20161 gtgcggcagg ctcaaccgcc ctgccgaatc tatggcatat ttcgacaaag ccctggccga
    20221 ccccacctac ccgacccctt atattgccaa cctgaataaa ggtatatgca gcgcaaaaca
    20281 ggggcaattc ggattggcgg aagcctattt gaaacgttcc ctcgccgccc agccgcagtt
    20341 cccacccgca tttaaagaac tggcgcgcac caaaatgctg gccgggcagt tgggcgatgc
    20401 cgattactac tttaaaaaat accaaagcag ggtagaagtc cttcaggccg atgatttgct
    20461 gctaggctgg aaaattgcca aagccctcgg caacgtgcag gcggcatacg aatatgaagc
    20521 acaattgcag gcaaatttcc cctactcgga agaattgcaa accgtcctca ccggtcaata
    20581 aacagattca aactatatga acacactcca acgccgcaag acgcatcaag tcctcatcga
    20641 tcatattacc gtcggttcag aagcacccgt cgttatccaa tctatgacca acaccgacac
    20701 tgccgatgca aaagccaccg cattgcagat taaggaattg agcgatgccg gctcggaaat
    20761 ggtgcggatt accgtcaaca gccccgaagc cgcgtccaaa gttgccgaaa tccgccgccg
    20821 cttggacgat atgggctatg ccacaccact gattggcgat ttccatttta acggcgaacg
    20881 cctgttggcg gaatttcccg aatgcggcaa agcattgtcc aaataccgca tcaatcccgg
    20941 caatgtcggc aaaggcgtaa aaggcgatga aaaatttgcc tttatgattc ggactgctgc
    21001 tgaaaacgat aaagccgtcc gtatcggcgt aaactggggt tctttggatc agagcctcgc
    21061 caaacgtatg atggatgcca acctcgtttc ttccgcgccg aaaccgcccg aagaagtgat
    21121 gaaggaagca ctgattgtct ccgctttgga atctgccgaa aaagccgttc tattgggact
    21181 gcccgaagac aaaatcatcc tgtcgtgcaa agtcagcgcg gttcatgatt tgattcaggt
    21241 ttaccgcgaa ctgggcagcc gttgcgtcta tccgctgcat ttgggtttga ccgaagccgg
    21301 tatgggcagc aaaggcattg tcgcatcaac ggcggcatta tctgttttgc ttcaagaagg
    21361 aattggcgac accatccgca tttcactcac tccggaaccg ggcagcccgc gtactcagga
    21421 ggtcgtcgtc gggcaagaga ttttacagac tatgggtttg cgctcgttta cgccgatggt
    21481 taccgcctgc ccgggatgcg ggcgtaccac cagtaccgta tttcaagagc tggcacaaga
    21541 tgttcaaaat tacctgcgcc aaaaaatgtc tatatggcgt accctttatc ccggggttga
    21601 atccctgaac gttgccgtaa tgggctgcgt tgtcaatggc cccggagaaa gcaaattggc
    21661 cgacatcgga atcagccttc ccggtacggg ggaaacaccc gtcgcacctg tttatgtaga
    21721 tggggagcgc aaagtcacac tgaaaggtaa taatattgca agcgaattcc tagctattgt
    21781 agaggaatat gttaaaacca attatggcaa aaacagttct aaacgcaata aaggaaaagt
    21841 catcccgata cagtctctat aaaacaaatg ccgtctgaac gtttcagacg gcatttgttt
    21901 ctctcagatt tcttattaaa gccgcaaaga agcgcggttt tccaaaactt ggtcgatcaa
    21961 accatattct tttgcttctt cggcagacat gaaattatca cggtcggtgt cgcgctccaa
    22021 atctgccaaa tcgcggccgc aatgtttcgc catcaggcgg ttgagttttt ctttgatttt
    22081 caacaactcg cgtgcgtgga tttcaatgtc ggatgcctga ccgcccaagc cgccgctgat
    22141 taaaggctgg tgaatcataa tccggctgtt gggcagggcg aaacgtttgc ctttctcgcc
    22201 tgccgacaat aagaacgcgc ccatacttgc cgcctgcccc aagcacaaag tcgatacatc
    22261 gggcttgatg aaattcatcg tgtcgtaaat cgacataccg gccgttaccg agccgccggg
    22321 ggaattgatg tagaagaaaa tatccttatc cggattctca ctttccaaaa acaacagttg
    22381 ggcgaccacc agattagcag actcatcggt taccgggcca accagaaata cgatgcgctc
    22441 tttcaaaagc cgggaataga tatcgaatgc acgctcaccg cgaccgctct gctcgataac
    22501 ggtagggaca agatggttat caaaagacat ttcgtctcct ttcatgatgg aaaagcacca
    22561 aagcgggctt taaaaacggc ttcggtgctt tcaaaaactg ccttcagaca gcattttcag
    22621 aacaatcagg cttgcgcgcc catcacttcg tcaaaagaca aagctttttt gtttactttg
    22681 gctttgccca aaacgaaatc aacaacgttg ctttctactg ccaaagaagt cggggcttgc
    22741 aggcgggaag tatctgcgta gtaccagtca atcacttctt gaggatcttc gtagctttct
    22801 gcgaagttgg cgacaacggc tttgatttgc tcttcagtcg gttccagttt gttttcgtca
    22861 accagtttgg ccaaaatcag acccaaagat acgcgacgtt cggcttgttc tttgaacata
    22921 tccaaaggca aatccaagtt cgcggcatcg gtcatacctt ggttaacgaa gttttgtttc
    22981 atttcgtttg ccaggcgggc ggcttcttca ttgaccaaag caaccggaac tttcaactct
    23041 acggctttaa tcagcgcgtt cattacggat tctttggttt gttcgttcac gcggcgttcc
    23101 acttcgcggc ttacgttttt cttcacttct tcacgcattt tggcaacatc gccatccgca
    23161 atacccaagg cttttgcaaa atctgcatcg acttcaggca gagtgggctc ggaaacattg
    23221 ttcagcgtaa tggtgaacac ggcagtttta ccggcaacat ctttgccgtg gtattcttca
    23281 gggaagttga cggtaacgtc tttactttcg cccgctttca tgccgactac gccggcttca
    23341 aattcaggca gcatttgacc tgcgcccaat acgaaggcgt agtttttgga tgcgccgccg
    23401 gcaaaaggtt cgccgtcgat tttgccttca aagtcgatga tgacgcggtc gccgtttcgg
    23461 gcttcgcggt caacatggtt gaagcgggta cgttgtttac gcaggatttc tacagtttgg
    23521 tcgacttcgg catcgccgac ggaagcggtt actttttcga cctcttgtgc agacaaatcg
    23581 ccgataacga cttcggggaa cacttcaaaa atggcggcaa ctttgaaaga ctctttatcg
    23641 tcttgttctt caacgccttc aaaacgtgga tagcctgcca ctttcaactc ttgggcaacg
    23701 gcaacatcgt agaagcggcg ttgcaccagc tcgttgatca cgtcgttttg cgcgctcgca
    23761 ccgtacattt gggcaatcat ttttaaaggt gctttacccg gacggaaacc gtcgattttt
    23821 gcacggcgtt gggtttgttt cagtttttta tcggtttctg cgttgatttc ggaccaaggc
    23881 agggacaaca ctactttgcg ttccagattt tctaaaattt caacagttac gctcatcata
    23941 agcccttaaa tttgttgtgt tgataaaatg ataaaccttc ttccctacat ggggaagcaa
    24001 acagcgcaac ggtacgatat ttgaaccgca ttgccgcaaa ggggaaattt tagctggcaa
    24061 gtatatcaca atgtttcgcc tgaaacataa tatgccgaga cctttgcaaa aatccccaaa
    24121 atccc
//
LOCUS       DAWTHD010000017        23343 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWTHD010000017 DAWTHD010000000
VERSION     DAWTHD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23343
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.017"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..329
                     /locus_tag="WM704_000563"
     CDS             <1..329
                     /locus_tag="WM704_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9340049.1"
                     /translation="AEPETLLPAVKKKIMPDGIVYTDSPGSRGKLDAGGFTRCRINRS
                     KEFADRRNHINGIGNFWNQAKRALRKYNGIDRKPFPPLLRECEFRLNFGTPSRQLKIL
                     RDRCGI"
     gene            693..2781
                     /locus_tag="WM704_000564"
                     /pseudo
     CDS             693..2781
                     /locus_tag="WM704_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2906..4456
                     /locus_tag="WM704_000565"
     CDS             2906..4456
                     /locus_tag="WM704_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFA9340050.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4880..6664)
                     /gene="lpdA"
                     /locus_tag="WM704_000566"
     CDS             complement(4880..6664)
                     /gene="lpdA"
                     /locus_tag="WM704_000566"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9340051.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGSAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6682..7116)
                     /locus_tag="WM704_000567"
     CDS             complement(6682..7116)
                     /locus_tag="WM704_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340052.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(7217..8779)
                     /gene="aceF"
                     /locus_tag="WM704_000568"
     CDS             complement(7217..8779)
                     /gene="aceF"
                     /locus_tag="WM704_000568"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFA9340053.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8929..11592)
                     /gene="aceE"
                     /locus_tag="WM704_000569"
     CDS             complement(8929..11592)
                     /gene="aceE"
                     /locus_tag="WM704_000569"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFA9340054.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            12289..14001
                     /locus_tag="WM704_000570"
     CDS             12289..14001
                     /locus_tag="WM704_000570"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFA9340055.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFCPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRGNEAQAVAIGEIVARVTA
                     SLNA"
     gene            14064..14720
                     /locus_tag="WM704_000571"
     CDS             14064..14720
                     /locus_tag="WM704_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFA9340056.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14730..15185)
                     /gene="ruvX"
                     /locus_tag="WM704_000572"
     CDS             complement(14730..15185)
                     /gene="ruvX"
                     /locus_tag="WM704_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFA9340057.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(15178..15726)
                     /locus_tag="WM704_000573"
     CDS             complement(15178..15726)
                     /locus_tag="WM704_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFA9340058.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15728..16288)
                     /locus_tag="WM704_000574"
     CDS             complement(15728..16288)
                     /locus_tag="WM704_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFA9340059.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGTGFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            16615..18417
                     /locus_tag="WM704_000575"
     CDS             16615..18417
                     /locus_tag="WM704_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFA9340060.1"
                     /translation="MQYKPLLLALMLVFSAPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEIAATKA
                     QISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKG
                     TEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEKEAAQQKAEARRAE
                     MSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVF
                     YSTAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAG
                     SKIGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            18544..20019
                     /locus_tag="WM704_000576"
     CDS             18544..20019
                     /locus_tag="WM704_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFA9340061.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            20127..20228
                     /locus_tag="WM704_000577"
                     /pseudo
     CDS             20127..20228
                     /locus_tag="WM704_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            20349..22376
                     /gene="uvrB"
                     /locus_tag="WM704_000578"
     CDS             20349..22376
                     /gene="uvrB"
                     /locus_tag="WM704_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HFA9340062.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(22554..23312)
                     /locus_tag="WM704_000579"
     CDS             complement(22554..23312)
                     /locus_tag="WM704_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFA9340063.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
BASE COUNT         5803 a         5864 c         6279 g         5397 t
ORIGIN      
        1 atgccgagcc tgaaacgttg ctccctgccg tcaaaaagaa aatcatgccg gacggtattg
       61 tttataccga tagcccgggc agccgcggca agttggacgc gggcggtttt acccgttgcc
      121 gcatcaaccg ttccaaggaa tttgcagacc gtcggaacca cattaacggc attgggaact
      181 tttggaatca ggcaaaacgc gccttgcgaa aatacaacgg aatcgatcgt aaacctttcc
      241 cgccgttgtt gagggaatgc gaatttcgac ttaacttcgg cacaccgtcc cggcagctaa
      301 aaatcctgcg ggatcggtgt ggaatttagg gataatctag tacagccaca aaaataaacc
      361 aatatttaac agtcggatag ctccgcctga ttaagttttt cggtaatagt tgtgattatc
      421 aaatataatt gccgcccgaa atatccgata agtttgtttg ttttatcttt aattccccaa
      481 tacttaaaaa agagagcgtt ttcatgaaca aaaaacttgc cttaatgccg ctgttgattt
      541 tgagcgcgtt ttcatctgcc gccgacaata ctccgcagca tggcgaactc ggacaggttc
      601 atgtccgcgc cgatgccaag cgcgtcaaag ccgcgcattc ttactccatc gccagcgatg
      661 gcgacttgcg cgaccgcgta aatttgggcg tattgggtaa agccaacgcg tttaccgcgc
      721 cgattaccgt cgtcaactac gacgaacaag ccttcaacaa taccgaggcg cgtactttag
      781 tggatgccgt agccaaaaaa gacgcttccg tttgagagtt tggcggcgaa agcaacacgc
      841 tgaccggtct gtatttccgt ggttatcagc ttgatgcgcg ccaattcagc gtaaacggct
      901 tggcaggaat gtatggcacg caaggcacgg cgagcgtgca agtcggcttc gcacaactga
      961 ttaaaggcgc gtccaccgcc gtaaacggca taccctgaag gcgcggtatc cggttccgtc
     1021 aacatcgaaa ccaaaaaagc cgccgacgaa ggcaaccgca aaatcggttt ggcccggttc
     1081 ggcaacaacc gcgcccaagg taggttcgac ttagggcaac gcttcggcga aaacaaagca
     1141 ttcggcgtgc gtgccaacgg caaactgcgc cacggcgaca ccccgcgcca cggttacaga
     1201 gaagacaaca aagaatttgc cttaaatgcc gattatcgcg gcgaaaaact gcgcgtgacg
     1261 ttcgactcca tctacgcgaa acgcaaaatc aacggcggcc gcgcgcgtat gcaggatatc
     1321 caaaacgccg gcggacgctt gttcgacgcg cccgacggca aaatcaacct gctgccttct
     1381 tggaactggc aaaataccgt cggcgaaacc aatatgctga cgtttgaatg ggatgcgttt
     1441 gacaatacct aaattaccgg cggtatcggc tacaacaaag cgcgttatta cggtacgctg
     1501 atttccccaa ccgtttgcgg tacaagcggt gcgagcagcc aaaccaaaac ctgcatggct
     1561 gccaaccaat accacaccgg cacggcacgc ctgaccgacc aataccacac cggcacggca
     1621 cgcctgaccg accaatattt ccgtactttg agtatgaacc tgaccgcacg cggcgaattt
     1681 gaaaccggcc cggtcacgca taactggagt acggctttcg accgcgttat ccgccagcgg
     1741 aaaaccataa acggcagcgg gaacggcaac agcaagatag aagtgaaggc aaacgaaaat
     1801 attgcgaatc aattggcatc gttcaaagcc gactatccca attcttgggc gaactcggcc
     1861 aacttggatg ccaatatcaa agtcaacagc ttggctttgt ccgatacttt gggcttcgtt
     1921 gataacaaat accccctgac tttgggcgga cgtttccaag cggttgaata cactgataag
     1981 aagaaatcac agagcggcga tgccaaacgt ttcagcccga tgctgatggc ggcgtgggtg
     2041 ccgcaacctg atttggtcgt gtgcggcaac tatatggaag acttggagcg tgccgatatc
     2101 aaaaccgatg attccggcga aacgacaatg gcgaagccgc gcgtcagccg ccagtttgaa
     2161 atcggcgtgc gtaaaaactg gggcgatttt gtaaccacgt tgaacgcgtt ccaaatcaaa
     2221 cgcccgggct actggcgcgg caataccaaa aaaggtactg attttgcagc atataaagca
     2281 gcaggaggcg cagccggcga cgagcaaggt atggaacgca atcgcggtat tgagttcaat
     2341 gcctatgcca acctgttgaa caaaaccctg cgcccgactt tcggcgtgat gtatctgcaa
     2401 tcgtccgtga agaattatcc gaatgcgcgc gatatgctgg ttaacggcgt acaagtcgcc
     2461 aacccgcgcg tgattgtcaa aacaggcgtt gaacgggata caccgtttgc caaaggtttg
     2521 agcttgaacg gcaatgtttc gtatttcggc aagtcttatc aagacacgca aaaacaatac
     2581 gccttcccgt cctatacctt ggttgacgta ggcgcgcgct acaaaaccaa gctgggcaaa
     2641 aacaccctga ccgtcagcag ctcggtagaa aacctgttca acaaaaacta ctggcaggta
     2701 cagcgcggac aatttgaccg tagtttcgcc gtcgtcggtc tgccgcgtac ttactggctg
     2761 aaggcggaat tggatttcta agctgcctcc gtttccgaaa atgccgtccg aacaggtttc
     2821 agacggcatt tcttttaaag aaatatggcg ggatatgctt aaataatgct attctagtac
     2881 ctttgaaacc tgcttgtttg aagtgatgaa aaaacctttg atttcagttg cggcagtatt
     2941 gctcggcgtt gctttgggta caccttatta tttgggtgtc aaagcagaag aaagtctgac
     3001 gcagcagcaa aaaatattgc agaaaacggg ctttttgacc gtcgaatcgc accagtatga
     3061 tcgaggctgg tttacctcta cggaaacgac ggtcatccgt ctgaaacccg agttgctgca
     3121 taatgcgcag aaatacctgc cggataactt gaaaatagtg ttggaacagc cggttacgct
     3181 ggtaaaccat atcacgcacg gccctttcgc cggcggattc ggcacgcagg cgcacattga
     3241 aaccgagttc aaatacgcgc ctgaaacgga aaaagttttg gaacgctttt ttgggaaaca
     3301 agttccggtt tcccttgcca ataccgttta tttcaacggc agcggtaaaa tggaagtcag
     3361 tgttcccgct ttcgattatg aagaactgtc gggcatcagg ctgcactggg aaggcctgac
     3421 gggggaaacg gtttatcaaa aaggtttcaa aagctaccgc aacagctatg atgcgccctt
     3481 gttcaaaatc aagctggcag acaaaggcga tgccgcgttt gaaaaagcgc atttcgattc
     3541 ggaaacttca gacggcatca atccgcttgc tttgggcagc agcaatctga ctttggaaaa
     3601 attttcgctc gaatggaaag agggtgtcga ttacaacgtc aaattgaacg aactggtcaa
     3661 cctcgttacc gatttgcaga tcggcgcgtt tatcaatccc aacggcagca tcgcaccttc
     3721 caaaatcgaa gtcggcaagc tggctttttc aaccaagacc ggggaatcgg gcgcgtttat
     3781 cgacagcgaa gggcggttcc gtttcgatac gttggtgtac ggcgatgaaa aatacggccc
     3841 gctggacatc catatcgctg ccgaacacct cgatgcttct gccttaaccg tattgaaacg
     3901 caagtttgca caaatttctg ccaaaaaaat gactgaggaa caaatccgca atgatttgat
     3961 tgcggcagtc aaaggcgatg cttccggatt atttacccat gacccggtac taaatatcaa
     4021 aattttccgt ttcaccctgc ctcagggaaa aattgatgtg ggcggaaaaa tcatgtttaa
     4081 aggcatgaag aaggaagatt tgaaccaatt gggactgatg ttaaagaaaa ccgaggcaaa
     4141 catcagaatg agtattcctc aaaaaatgtt ggaagatttg gcggtaagtc aggctggaaa
     4201 tattttcagt gtaaatgccg aagatgaggc ggaagccaga gcaagcattg ccgatattaa
     4261 tgaaacattg cgcctgatgg tggacagtac ggtccaaagt atggcaaggg aaaaatatct
     4321 tactttagac ggtaatcaga ttgatacggt catttccctt aaaaacaacg ccctgaagtt
     4381 aaacgggaaa acgctgcaaa atgaacccga tcctgatttt gacgagggag atatggtttc
     4441 cggccagccg cattaatgcc gtttatgcca aatgccgtct gaaatgttca gacggcattt
     4501 ttgtcggtct cgaaatagga tggattttca tgtttcaaaa acaattgatt gtctctaatc
     4561 cgatatcagg acacgcttcc aatgtttaga tatggaattc ggacgggata ggggaactat
     4621 tgaaatccag ttgaaatctg ttattggaaa gcatggagga aaaataaaag gctgtatttt
     4681 aatatagtat tgataggcat gcttagcttc aaggtattca atagtgtagg tgtctgccta
     4741 actggaaaat aatcgtcttt tcccttgcaa ccagttaaaa ttgacagatg gttaaaacac
     4801 agccaaataa aaatgccgcc tgaagggttc agacggcatt tgttcggata aaccttatcg
     4861 gctgcttaga gcatcaggtt tattttttct tttgaggagg caggtcggta catacaccca
     4921 aagccacttc cgcagccata ccgatggatt cgcccaaggt cgggtgcggg tggatggttt
     4981 tgccgatgtc tgccgcgtcg cagcccattt cgatggcaag gcagacttcg ccgatcatgt
     5041 cgccaccgtt cggaccgaca atgccgccgc cgatgatgcg gccggtttcg gcatcgaaaa
     5101 tcagcttggt aaagccgttg tcgcaaccgt tggcaatcgc acgaccggaa gccgcccatg
     5161 ggaagttggc tttggtgatt ttgcggcctg atgctttggc agacaattcg gtttcaccga
     5221 cccatgccac ttcgggggaa gtgtaggcaa cgccgggaat aacgcgtgcg tcgaagtagg
     5281 ctttgtggcc ggcacagttt tcagcggcaa cgtgaccttc gtgaacggct ttgtgtgcca
     5341 acatcggttg accgacgata tcgccgatag cgtagatgtg cggtacgttg gtgcgcattt
     5401 gtttgtcaac ttcgatgaag ccacgatcgg taacggcaac accggctttt tctgcgctga
     5461 tgagtttgcc gttaggcgca cggcctgccg cgaccagtac ggcatcgtaa cgttgcggct
     5521 ctttaggtgc atttgcacct tcaaaggtaa cgtaaacgcc gtcttctttc ggctcgactg
     5581 caacggtttt ggtgttgacc ataatgttgt caaaacggta ttcgttttgt ttttgccata
     5641 ctttaaccaa gtcgcggtct gcgccttgca tcaggccgtc catcatttca accacatcca
     5701 ggcgagaacc cagcgtgctg taaaccgtac ccatctcgag gccgataatg ccgccgccga
     5761 taatcagcag tttgcccggt acttctttca gtgccagtgc gccgctggaa tcgatgatgc
     5821 gcggatcttc aggaatgaaa ggcagtttgg ttacgcggct gcctgctgca atgatacagt
     5881 ttttgaaggc aacgattttt ttctcgccgg taggggctgc ctgttcgtac gcgtcgccgg
     5941 cagtcagcga cacttccaag tggtgaggat ccaagaattg cccgtcgcct tggataacgt
     6001 ccactttacg gcttttcgcc atacctgcca aaccacccgt gaggcgggaa accacgccgt
     6061 ctttataggc gcgaagcata tcgatgtcga gttccggttc ggggtatttg ataccgttgg
     6121 cagccaagtg gcgcacttcg tcgataacgg cggcattgtg caacaaggct ttggaaggga
     6181 tacagccgac gttcaggcaa acgccgccca aagttttgta acgctcgacg atggcgactt
     6241 tcaagccttc atcggcagcg gcaaatgcag cggagtaacc gccgggaccg ccgcccaata
     6301 cgaccacgtc gtactcggca tcggcagcac tgccgaattg cgcggcttgc ggagcaggag
     6361 cggcagcttt gggtgcttct tgcgccgggg cggcagccgc ttcggcttta ggggcggcag
     6421 ccgcaccttc ggcttcgacg acgacaatca aaccgccttc ggagattttg tcgccgactt
     6481 tgactttcac ttctttgatc acgcccgcaa cttcggcagg cacgtccatc gtcgctttgt
     6541 cggtttccaa ggtaatcagg gtgtcgtcaa cggcgatggt gtcgccaacg ttcacttcaa
     6601 ccgcgataat atctacattt tcgtgtccgc caatgtcggg cactttcaat tcaactaaag
     6661 ccattatatt gacctttcaa cttagttaat ttctatcaac tctttagcaa taatcacttc
     6721 ttcaggaata cagccacaat cttcagctct gattgttact tccttctcat ctgggttttc
     6781 tggatgttca acctcatatt caaaaataga atattgacca aacaaatctc tatttaaaac
     6841 atatatatag ccattttcga tgccggagct tgttgcaaat ttcttggcaa tttccttgtc
     6901 tgttgtcgta gatatataac atccgtcata tagatctgtt tcaatttgat gggcgtaaac
     6961 tgcattcttc acacttggac catgtgtagc tttaccatca tatttaaact taccatcata
     7021 acgaattgca acttcagctt tattaccttt aggttttaac tgtccattat tttgctcatc
     7081 ttgttggcaa ctaatgcctc tatataaata agaaatttcc cataagttat taatttaaag
     7141 tttgaattta acaccatcac tcatcaatct caagcaatcc aaacattatc agactgcttg
     7201 agagggatgt tttattttat aaggtaatgc ggcggaagtc tttcaacagg ttcgccagga
     7261 atacggtgaa gcgcataccg gcggcaccgt cgatgacgcg gtggtcgaag gacaggctca
     7321 acgggcacat caggcgcggg gcaaactctt tgccgttcca aaccggtttg atttgggatt
     7381 tgcacacgcc caagatagcg acttcgggag cgttcacaat cggcgtgaaa cctgtgccgc
     7441 cgatgccgcc caagctggaa atggtaaagc acgcgccttg catttcttgc ggtttgagct
     7501 tgccttcgcg ggcttttttg gacaattcgg tcaattcttg gctgatttgt ttcaagcctt
     7561 tttgatccac gtctttgatg acgggaacaa ccaagccgtt cggcgtatcg gctgcgaaac
     7621 cgatgttgaa gtagtttttc agcaccaggt tgtcgccgtc caaagaagcg ttgaattcgg
     7681 ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa cgccaacggg gacagtttca
     7741 cgccttcgcg ttcccattct ttgttcagct gtttgcggaa ttcttccagc tcggtcatat
     7801 ccgcttcttc gtgtacggta acgtgcggaa tcaccaccca gttgcgggac aggttttgac
     7861 cggagatttt cttaatgcgg gacaattctt taacttcgac attgccgaat ttggagaagt
     7921 ccactttagg ccacggcagc aagtccagac cgccgcccaa agatgcgccg gctgcggcag
     7981 gttttgccgc gccgccctgc attacggatt tcacaaaggc tttgatgtcg tctcccatga
     8041 tacggccttt caagccgcta cctttgactt ggcccaaatc cacgcccaat tcgcgcgcca
     8101 gtttgcgtgc ggaaggaccg gcgtgtgctt tggcgaaagc ggcttcatcg attttggcag
     8161 cggcaggcga ggcagatgta ggcacggcgg cgggtgcggc agcttgagcg gcaggcgcag
     8221 gagctgctgc ggcagagccg gcggtttcca cttcgataat ggcagagcct tcggacactt
     8281 tgtcgccgac ttttaagaat acggctttaa cgacaccggc agcggtacaa ggtacgtcca
     8341 ttgtcgcttt atcggtttcc aaagtaatca gcgtgtcgtc ttcggcaacg gtgtcgccaa
     8401 ctttgatttc aacggcgatt acatctacat cggtatggcc gccgatatcg ggaacggcta
     8461 cttgaacggt tgcaccgcct gcggcagcgg gtgcaggttg tgcttcggcg gcagcagccg
     8521 gggcggcttc ggcagcggcg gcaccggttt caacggtcaa aattacgccg ccttcggaga
     8581 ttttgtcgcc gactttgact tttacttctt tcacgacacc ggccgcatcg gcaggcacat
     8641 ccatcgtggc tttgtcggtt tccagtgtaa tcagggtgtc gtcaacggcg atggtgtcgc
     8701 ccgctttaac ttctacggcg atgatgtcga cgctttcgtg accgccgata tcggggactt
     8761 tgatttctac gatactcatt tgagttcctt taatcttttt cagttggatg ccgtctgaaa
     8821 gctgcttcag gcggcatcgg tacggtttgt ctgttcaaac cattcgggca ttcggttttc
     8881 agacggcctg atgttggcat gaaacaaaca ggccgtctga aacatcaatc agcgtttcca
     8941 gctaggggct gaatcggctt tgatgccgta tttctcaatg gcttgttgaa cggtttcttt
     9001 gctgactttg ccttgttccg ccaatgcggc caatgctgcc acggcaacgt tgtagcggtc
     9061 cacttcaaag aagcggcgca ggttggcgcg gctgtcggaa cggccgaaac cgtcagtgcc
     9121 caagacatgg tagtcgttcg ggatgtacgc gcggatacgg tcggcatagc tgcggatata
     9181 gtcggtagcg gcgataaccg gaccgtcatg accttgcagt tgggaagtaa cgaaaggcac
     9241 tttttcagct tccagcggat gcaggcggtt gaagcgttcg gtttcgatgg cgtcgcggtg
     9301 cagcaggttg aaggacgggc aagaccagat gtctgcttcc acgccgaagt cggctttcag
     9361 cagctcggca ccggcaatca cttcttgcag aatcgtaccg gaacccatca attggacttt
     9421 cttgtcgcct ttgccgccgg ctttcagcag gtacatacct ttgaggatgt cttgttccgc
     9481 accttccggc atatcgggat gggtgtagtt ctcgttcatc agggtgatgt agtagaacac
     9541 gtcttcatta ttggcataca tacggcgcag accgtcttgt acgatgacgg ccacttcgta
     9601 ttggaaggtc gggtcataag atacgcagtt cggaatcagg tcggcctgga tgtggctgtg
     9661 gccgtcttcg tgttgcagac cttcgccgtt cagcgtcgta cggccggcag taccgcccag
     9721 caggaagccg cgcgcgtgca tatcgcccgc cgcccaagcc aagtcgccga tacgttggaa
     9781 accgaacatg gagtagtaga tgtagaacgg aatcatcgcg aagtcgctgt tggcgtagct
     9841 ggtcgcagcc gcaatccagt cggccatcgc gcccggttcg ttaatacctt cttgcaagat
     9901 ttgaccgtcg acggattctt tgtagaacat cagttggtct ttatcttgag gcgtgtattg
     9961 ttggcctttg ggattccaaa taccgtattg gcggaacata ccttccatgc cgaaagtacg
    10021 gctttcgtcg ggaacgatag gtacgacgcg tttgccgatt tttttgtctt tcagcaaagt
    10081 ggacaggatg cggacgaatg ccatcgtggt cgagaactcg cgttcaccgc tggatttgag
    10141 ttgtgcgtcg aatgccgaca gctcgggcac ttccaatact tcctgcgtcg gtttgcgttg
    10201 cggcaggtag ccgcccaaag catcgcggcg tgcgtgcagg tatttgtatt cttccgtatc
    10261 gggggcaaaa gtcaggtaag gcagatcgcc gctttcgatt tgttcgtcgg taaccggaat
    10321 gtcaaagcgg tcgcggaatt gtttcaggga cgctttgtcc atttttttgg cttggtgggc
    10381 aacgttctga ccttcgccgg atgcgcccat accgtaacct ttaatggttt tcgccaagat
    10441 aacggtaggt ttgccgtcgg catggttcgc tgcgcggtcg taggcgttgt acactttttg
    10501 aggatcgtgg ccgccgcggt tcaatgccca gagttgctca tcggtcatat cggcaaccaa
    10561 tgctttcagt tcgggcgtat tgaagaagtg ttcgcgcaca tacgcgccgt ctttggattt
    10621 gtaagtttgg tagtcgccgt ccaaacattc ttccatacgt tggcgcagga taccgtcttt
    10681 gtctttcgcc aagaggcggt cccaacggcg gccccaaatg actttgacga cattccagcc
    10741 ggcaccggca aagttgcctt ccaattcttg gatgattttg ccgttgccgc gcaccggacc
    10801 gtccaagcgt tgcaggttgc agttgatgac gaaaatcagg ttgtccaagc cttcgcgtgc
    10861 agccagcgcg attgcacctt gggattcggg ttcgtccatt tcgccgtcgc cgcagaaaca
    10921 ccatacttta cggcctttgg tttttgccaa gccgcgtgat tccaagtatt tcaggaaacg
    10981 cgcctgataa atcgccatga tggggcccaa gcccatggat acggtcggga actgccagaa
    11041 gtcgggcaag aggtgggggt gtggatagga aggcagaccg tgtccgtcca cttcttggcg
    11101 gaagttgttc agctggtctt cggtcagacg gccttcgacg aatgcgcgtg catagatgcc
    11161 cggggcaacg tgaccttgga agaagaccaa atcgccttct tcaccttcac ctttggcttt
    11221 ccagaagtgg ttgaaaccga cttcgtacat ggtggcggca gattggaaag atgcgatgtg
    11281 cccacccagt tccaaatctt tcttgccggc gcgcaatacg atggcggcgg cgttccagcg
    11341 tacgaatgcg cgaatgcggt gttcgatgtt ttggtcgccc ggaatgcctt tttcgttttc
    11401 aaccgaaacg gtattcaaat acggggtggt cgtgccgtgg ggcatacgta cgcctttgtc
    11461 gcggcagtat ttgagcaggt tttccaagag gtattgcgcg cgttcgccgc cttcatattc
    11521 gaggacggag cttaacgcgt ccagccactc ttgggtttcg atagggtcaa catcgtgtaa
    11581 ttgggtggac atagtatcta tcctttatgt tgagtgttta atcggaaggg agggtgtagc
    11641 caaccccttt attttcgttt gtgaaaaata ataagcaaaa acgaaaattt ctaaatcaaa
    11701 ccgaaacaaa ataatttaag tttaatgttt tcaaaaaatt ataaaagtct tggaagcaag
    11761 ggaaaacttg aaaaaaaatg ttcggtctgc tccgcacgaa atcctatcag caatcgggtc
    11821 tgctggcaaa taaccatgct tttaaaagca ggttttatac tgaagttggc gtttggtaag
    11881 gacaggtctt tttgattgcg aaaacattct gtttaatttt ggttatattt tttcggaatt
    11941 gtgggatatc gaatcgttat gtctctgtcg gcaattttcc tattgcagaa aatctataca
    12001 caagctttgt ggacaaccgt gttgataagc cacggcttgt gttaaaaaag tcttacgcaa
    12061 caaatgtacg cttattttga ttaaaaatta ggcaaattta aatctataaa aatcatgtaa
    12121 ttatgttcaa tatgatgttt ctggtcatgt ttttgccgtc tgttatgggg tttgtcccgc
    12181 gtttggggca gggggtgaat tttggaggtt ggtgcaaaac ctgctttttt cgcgttacaa
    12241 tctgcacccg atttactttt gaatctgcct gtttggaaaa atacttttat gaaagccagc
    12301 caattcttta tctctacttt aaaagaagcc cctgccgaag ccgcgtttgc cagccacaag
    12361 ctgatgattc gcgccggtct gattaaagcc aacgcgtccg gtctttatac ttggatgccg
    12421 atggggctgc gcgtgttacg caaagtcgaa aacgtcgtgc gcgaggaaat ggcgcgcgcg
    12481 ggcagcgtgg agctgctgat gccggtggtg cagcctgccg aactgtggca ggaatccggc
    12541 cgctgggagt tttacggtaa agaactgctg cgcctgaaag accgccacga acgcgatttc
    12601 tgcatgggcc cgacctgcga ggaagtcatc gccgacatcg tgcgcaaaga aatcaacagc
    12661 tacaaacaac tgccgaaaaa tttttaccac atccaaacca aattccgcga cgaagtgcgc
    12721 ccgcgtttcg gcgtgatgcg cgcgcgcgaa tttgtgatga aagatgctta ttccttccac
    12781 gccgactacg cctcgcttca ggcgacctat gatgccatgt atgacgctta ctgccgcatc
    12841 tttacccgtc tgggtttggc attctgcccc gtcgccgcag acaccggcag catcggcggt
    12901 acgggttcgc acgagtttca agtgttggcg gaaagcggcg aagatgtgat tgcatacagc
    12961 gacacttccg attacgccgc caatatcgag ttggcaccga ccttgccgct taaaggtgaa
    13021 cgtgccgccg ctcaggctgt gttgaccaaa gtacatacac caaacgtcaa aaccattgag
    13081 tctttggttg aattcctgaa tattccggtt gaacaaaccc tcaaatccat cgtggttgaa
    13141 ggcgaaaacg aaggcgaact cgtcctactg ctgttgcgtg gcgaccatga gtttaacgac
    13201 atcaaggcag aaaaactggc gggcgtaaaa tcgccactga ctatggcaag ccctgccgcg
    13261 attgttgaac aattcggcgc aaacggcggt tcgctcggcc ccgtcggctt cacaggcaaa
    13321 gtctatgccg atttcgctac cgaaaaaggc gcggactggg ttatcggcgc aaacgaagac
    13381 gactaccact ataccggctt caacttcggc cgcgatgccg ccgagcctga attcgtcgat
    13441 ttgcgtaatg tggtcgaagg cgacgaaagc cccgacgggc aaggccgtct gaaactggcg
    13501 cgcggcatcg aagtcggaca tgtcttccag ttgcgcggca aatataccca agctatgaac
    13561 gtcagcttcc tcgacaataa cggcaaatcg caaatcatgg aaatgggctg ctacggcatc
    13621 ggcatcaccc gcgtcgttgc cgccgccatc gagcagaata acgacgaaaa aggcatcatt
    13681 tggaccaaag cgatggcgcc gtttgaagtc gtcatcgtgc cgatgaacta caaaaaatcc
    13741 gacactgtgc gtgaagccgc cgacagaatc tatgccgaat tgctggcggc aggcgcggat
    13801 gtgctgctgg acgaccgcga cgaacgcgca ggcgtattgc tgaacgattc cgagcttctc
    13861 ggtatcccgc accgcatcgt catcggcgac cgcgccttga aagaaggcaa tgtcgaatac
    13921 gccgaacgcc gcggcaacga agcgcaggca gttgcaatcg gagaaattgt tgcgcgtgta
    13981 acagcttcat taaatgcgta agtctggtca aatgccgtct gaaaataatt tcagacggca
    14041 tttttatttt gggggaaagt gaagtggctt cagtattttt agaaggagaa gcagtcgagg
    14101 tcggcaatat tttctgtatc ggcaggaact atgccgcaca tgtcgaagag ttaaaaaacg
    14161 aaataccttc agaacctgtg gtgtttatga agccgtcagg cagcattttg aacagcggcg
    14221 gtacaatctt gctgcccgag ttcagtaggg atgtgcagtt tgagtgcgaa ctagtgttgc
    14281 tgattggccg ggattcagac ggcacgggcg agggtgaaga tattttggga tgcgtggcgg
    14341 ggtacggcgt gggtttggat ttgaccgcgc gggacatcca atgccgtctg aaggcgcagg
    14401 ggctgccctg gctgaaggca aaagggttca ggcattctgc gtgcgtgtcg gattttgcgg
    14461 cggcagacag aatcggcaat caagataagg tgttgttttc attaaaacaa aacggcgtat
    14521 tgaaacagcg cggggagact ggtttgatga tttacccgat tcgggaaatt ttacacaaac
    14581 tggcggcgga ttacggtttg gggaaagggg atttggtgtt taccggaacg ccctccggcg
    14641 tgggcgcaat cggtgcgggg gacaatttgg cattggagct ggacggcttg gtgtgcgcct
    14701 ctttcacgat agggtgttaa ccgcgccgct taaccttcac gcccgttgaa acattccgcc
    14761 gggccgccct cgagaaaacc gtgcaggatg gcttgcgccg ccacttggtc gagcaccgat
    14821 ttgcgttttt tgcccaagac ctgtgcttcc gaaagcaggc tttcggcata gacggacgac
    14881 agccgttcgt caacccaata gacggggaga ttgaacctgc cgttcagcct gcgtccaaac
    14941 ttgcgcgaca ggtgcgtcat ttcatgttcc gtgccgtcgg catgcacggg cagtccgacg
    15001 acaaaataac gcggctgcca ttcttgaacc agcttggcga ttgccgcgaa tttttcatcg
    15061 ttgctgccgc cggtaacggt ggccagcgga tgggacagcc ccaattccgc atcgccctgc
    15121 gccacgccga tgcgcgcttc gccgaagtcg aatgccagtg ccgttccttt tggaatttta
    15181 tgcatggccg gctcccgaaa acagggcgag cgggtcgatg ccgagtttgg cgaatgcggc
    15241 ggcgtaacgg tgttcgtagg ggatgtcgaa caggatgtgt tcgtcggcgg gaacggtcag
    15301 ccacgcattg tcggcaagtt cgcgttcgag ctgccctttg ctccagctcg aatagcctat
    15361 gctgatcaag gctttgtcaa ccgcgccttc gcgtgaaatg ttttcaagca catcgcggga
    15421 agaggtcagc gcgatgccgt ctgaaacgcc gatgctgctt tgccagttgc cgatcggagt
    15481 gtggacgacg taaccgcgct cgacctgcac cggcccgccc atcatcacgc tgtcgtgctg
    15541 catccgcatg gggatgtttt tgccggtggc ggaaaaaatc atgtccatcg taatcggaga
    15601 gggtttgttg atggcgatac cgagtgcgcc gtcttcatcg tgtttgcaga tatagacgac
    15661 cgattgtgaa aaaaacgcgt cttccatatc gggcatggcg acgagaaagt ggttggacag
    15721 gttcatatca gcgtttgtcg ggatttttta tcgggttgga aataatgcag gcgccggcaa
    15781 gcagtttgcc ggaagtatcg gcttgcaccc cgtcggtttg cggtatgccg ctgccgaaac
    15841 aggaaaccgc gcttaaggaa tttttcggcg atccttggac gattttatcg ctgtaaacca
    15901 aataggcgaa ggctttgcgt ttggggtcgt aataacggac aatctgccgg ctcttgaacg
    15961 cgaagcccgt accgcgcttg aaaacttctt tcggtttgcg cacggcggtt tcgtcaaaag
    16021 aaatcgaaga tgccgtctga acgcacgaaa ccgatgcgtc ggacgcgtcc tcttccaaat
    16081 tgaccatttc cttcaagccg cctttttttg catacgaaat ataacaggca accccttgaa
    16141 cgtcgggatc gtcgaatcct tccacttcga tacggtcgtt tttgcccaac atgttgaaaa
    16201 cggtacttgc ccgtccgatt ttatcggttt cgccgctgcc gcaggcagtc ggcaggacgg
    16261 cggcagacag cagtagcagt ctgttcatca aaatttcctt atcggttgaa tccccgtttt
    16321 ccggaaggcg gcagggtttg ctttgtttgg aggggcgcac gctcccaaat cgggcaacat
    16381 acggtacgca gcttacatgt gctaccctgt gtgtcggaac acaatatccg ttgcagcatt
    16441 gaaaatatgc cgcacaatat aaggctgttt gagccgattg caagtatccg cgcaaagaag
    16501 gtgcaagatt gggcaaacgg cattttcaga cggcataact gacagtataa tccgaacatc
    16561 cggccgcttt cgcgcggcct tcagcattat ccgatttttt ctgaaagccg aaccatgcaa
    16621 tacaaacccc tcctgcttgc cctgatgctt gtcttttccg cccccgccgt tgccgcccac
    16681 gacgcggcac acaaccgttc cgccgaagtg aaaaaacagg cgaagaacaa aaaagaacag
    16741 cccgaagcgg cggaaggcaa aaaagaaaaa ggcaaaaatg ccgcagtgaa agataaaaaa
    16801 acaggcggca aagaggcggc aaaagagttc aaaaaaaccg ccaaaaaccg caaagaagca
    16861 gagaaggagg cgacatccag gcagtctgcg cgcaaaggac gcgaagggga taaggaatcg
    16921 aaggcggaac acaaaaaggc acatggcaag cccgtgtccg gatccaaaga aaaaaacgca
    16981 aaaacacagc ctgaaaacaa acaaggcaaa aaaggggcaa aaggacaggg caatccgcgc
    17041 aagggcggca aggcggaaaa agacactgtt tctgcaaata aaaaagcccg ttccgacaag
    17101 aacggcaaag cagtgaaaca ggacaaaaaa tacagggaag agaaaaatgc caaaaccgat
    17161 tccgacgaat tgaaagccgc cgttgccgct gccactaatg atgtcgaaaa caaaaaagcc
    17221 ctgctcaaac aaagcgaagg aatgctgctt catgtcagca attccctcaa acagcttcag
    17281 gaagagcgta tccgccaaga acgtatccgc caagagcgta tccgtcaggc gcgcggcaac
    17341 cttgcttccg tcaaccgcaa acagcgcgag gcttgggaca agttccaaaa actcaatacc
    17401 gagctgaacc gtttgaaaac ggaaatcgcc gctacgaaag cgcagatttc ccgtttcgta
    17461 tcggggaact ataaaaacag ccagccgaat gcggttgccc tgttcctgaa aaacgccgaa
    17521 ccgggtcaga aaaaccgctt tttgcgttat acgcgttacg taaacgcctc caatcgggaa
    17581 gttgtcaagg atttggaaaa acagcagaag gctttggcgg tacaagagca gaaaatcaac
    17641 aatgagcttg cccgtttgaa gaaaattcag gcaaacgtgc aatccctgct gaaaaaacag
    17701 ggtgtaaccg atgcggcgga acagacggaa agccgcagac agaatgccaa aatcgccaaa
    17761 gatgcccgaa aactgctgga acagaaaggg accgagcagc aactgaacaa gctcttgagc
    17821 aatttggaga agaaaaaggc cgaacaccgc attcaggatg cggaagcaaa aagaaaattg
    17881 gctgaagcca gactggcggc tgccgaaaaa gccagaaaag aaaaagaagc ggcgcagcag
    17941 aaggctgaag cgcgacgtgc ggaaatgtcc aacctgaccg ccgaagacag gaacatccaa
    18001 gcgccttcgg ttatgggtat cggcagtgcc gacggtttca gccgtatgca gggacgtttg
    18061 aaaaaaccgg ttgacggtgt gccgaccggg cttttcgggc agaaccggag cggcggcgat
    18121 gtttggaaag gcgtgttcta ttccactgcg cctgcaacgg ttgaaagcat tgcgccggga
    18181 acggtaagct atgcggacga gttggacggc tacggcaaag tggtcgtgat cgatcacggc
    18241 gagaactaca tcagcatcta tgccggtttg agcgaaattt ccgccggcaa gggttatacg
    18301 gtcgcggcag gaagcaaaat cggcacgagc gggtcgctgc cggacgggga agaggggctt
    18361 tacctgcaaa tacgttatca aggtcaggtg ttgaaccctt cgggctggat acgttgatcg
    18421 gattgccgaa acggcaggct ttatgcaaat cgtgccgata tggtgcgctg ttccgtgaag
    18481 tttgccgttt gccgtacctg tgttttccgg tttttggaaa tcaagagaga aagttaaaga
    18541 agaatgtcga aacctgtttt taagaaaatc gcactttata ctttgggtgc aatcagcggc
    18601 gtggccgtaa gtctggcggt gcagggtttt gccgccgaga aggacgggcg ggataacgaa
    18661 gtcctgccgg tgcaatccat ccgtacgatg gcggaggttt acggtcagat taaggcaaac
    18721 tactatcatg acaaacccga tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc
    18781 ggtttggatc cgcattccga atatatggat aaaaaaggtt atgccgagat aaaggagtcc
    18841 accagcggcg aatttggcgg cttggggatg gaaatcgggc aggaagacgg ttttgtcaaa
    18901 gtggtttcgc cgattgagga cacgcctgcc gaacgggcgg aggtgaaaag cggcgatttc
    18961 attgtgaaaa tcgataatgt ttcgacgcgc ggtatgacgg tcagcgaagc ggtgaaaaaa
    19021 atgcggggca agccgggtac gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag
    19081 ccgatagtcg tcaacctgac ccgtgccatt attaaagtga aaagcgtccg ccatcacctg
    19141 atcgaacccg attacggcta tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc
    19201 gtcaataccg ccgcaaaaga gctggtaaag gaaaataaag gaaaaccgct caaggggctg
    19261 gtgttggatt tgcgcgacga ccccggcggg cttttgaccg gcgcggtcgg cgtgtcggcg
    19321 gcgtttctgc cgtctgaagc ggtcgtcgtc agcaccaagg gacgcgacgg caaagacggc
    19381 atggtactga aagccgttcc cgaggattat gtgtacggta tgggcggcga ccctttggcg
    19441 ggtattcctg ccgagttgaa aacgattccg atgacggtat tggtcaattc cggttcggct
    19501 tccgcgtcgg agattgtcgc cggcgcattg caggaccaca aacgcgcggt catcgtcggt
    19561 acgcagagct tcggtaaagg ttcggttcag actttgattc ctttgtccaa cggcagcgcg
    19621 gtcaagttga cgaccgccct gtattacacg ccgaacgacc gttccattca ggcacagggg
    19681 attgttcccg atgtcgaagt aaaagataag gaacgtactt ttgaaagccg cgaggcggac
    19741 ctggtcggac acatcggcaa tcccttgggc ggcgaggatg tgaacagtga aacccttgcc
    19801 gtaccgcttg aaaaagatgc ggataagccc gctgcaaaag aaaaaggtaa aaagaaaaag
    19861 gacgaggatt tgtcttcaag gcggattccg aaccctgcga aagacgatca gttgcgtaag
    19921 gctttggatt tggtcaagtc gcccgagcag tggcagaagt ctttggggct ggcggcgaaa
    19981 aagccggttt caaataaaga taaaaaagat aagaagtagg ttttgacgat gccgtctgaa
    20041 agggtttcag acggcatatt atgtttgaaa ggacaggtta tgagcgattt tttaccggaa
    20101 tttgaaccgt gcggcattta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
    20161 agacagtaca aatagtacgg caaggcgagg taacgccgga ccggtttaaa tttaatctac
    20221 tatacttggg tagcgattgg gtttcagacg gcatacattc aggatttcac cgccttatac
    20281 cgtctgaaag acgtttctga cccatcccta ctacacgctt tgtccgcaaa cgtatcacag
    20341 gcaagattat ggaagtcatc cgatacccaa attccccctt caaactccac caacccttcc
    20401 cgcccgcagg cgaccagccc accgccattg cgggcctgct cgaagggctt tcagacggcc
    20461 ttgcctacca aaccctgctc ggcgtaaccg gttcgggcaa aacctacacg atggcgaacg
    20521 tcatcgcgca aagcggcaga cccgccatca ttatggcgca caacaaaacc cttgccgccc
    20581 agctttatgc cgaaatgcgc gagtttttcc ccgaaaacgc agtggaatat ttcgtgtcgt
    20641 attacgacta ttaccagccc gaagcctatg tgcccagccg cgatttgttc atcgaaaaag
    20701 acagcgcgat caacgaacac atcgagcaga tgcgcctttc cgccaccaaa aacctgatga
    20761 cgcgcgacga cgtgattatc gtcgccaccg tgtccgccat ttacggtatc ggcgacccga
    20821 ccgagtatca acaaatggtg ctgtccgtaa aagaaggcga caccatcgag cagcgcgaca
    20881 tcatcgccac gctcgtttcc atgcagtacg aacgcggcga tttggacttc aaacgcggca
    20941 gcttccgcgt gcgcggcgac gtgattgacg tgtaccccgc cgaaagctcc gaaaacgcct
    21001 tgcgtatcag cctgtttgat gacgaaatcg accgcctcga tatgttcgac ccgctttcag
    21061 gcagcctgca ccagcgtgtc ggacgctaca ccgtcttccc gtccagccac tacgttaccc
    21121 cgcgcgacac cgtattgcgc gcctgcgaat ccatcaaaga agaattgcgc gaacgcatcg
    21181 aatttttcgc tcgcgaacaa cgccccgtcg agcaacaacg catcgaacag cgcacccgtt
    21241 tcgacctcga aatgctctac gaaatgggct tctgcaaagg cattgaaaac tactcccgcc
    21301 acttctccgg taaaaaagaa ggcgaaccgc cgcccacgct gatggactac ctgcccgaca
    21361 acgccatcat gttcatcgac gaaagccacg ttaccgttac ccaaatcggc ggcatgtaca
    21421 aaggcgacgc atcgcgcaag caaaacctcg tggactacgg cttccgcctg ccttccgccc
    21481 gcgacaaccg cccgctcaaa ttccacgaat ttgaaaaagt catgccgcaa accgtcttcg
    21541 tttccgccac ccccgcgaaa tacgaagaag aacacgccgg acaagtcgtc gaacaagtcg
    21601 tccgccccac agggctggtc gacccccaaa tcatcatccg ccccgtcgcc acccaagtcg
    21661 acgatttaat gagcgaaatc aacgaccgta ttcaaaaagg cgaacgtgtg ctcgttacca
    21721 ccctcaccaa acgcatggcg gagcaactca ccgactatta cagcgaactc ggcatcaaag
    21781 tgcgctacct gcacagcgac atcgacaccg tcgagcgcgt tgaaatcatt agagatttgc
    21841 ggctcggcct gtttgacgta ctcgttggca tcaacctgtt gcgcgaaggc ctcgacatcc
    21901 ccgaagtgtc cctcgtcgcc atcctcgatg ccgacaaaga aggcttcctg cgctcccacc
    21961 gcagcctgat tcaaaccata ggccgcgccg cgcgcaacgt gaacggcgtc gccatcctgt
    22021 acgccgacaa aattaccgac tccatgaaag ccgccgtcga cgaaaccgaa cgccgccgcg
    22081 aaaaacagat taaattcaac gaagaacacg gtatcgtgcc gcagcagatt aaaaaacagg
    22141 tcaaagacat catcgacggc gtgtaccacg aagaagacag tggcaaaggc cgtagacaag
    22201 gcaaaaacaa ggtcaaagtc ggcgaaatcc acaacgaaga agacgcgatt aaagaaatcg
    22261 ccaaactgga aaaagccatg cagcaggcgg ctagggattt acagtttgaa gaagctgctg
    22321 tgttgaggga taggattagg aatattaaag agaatttgtt gtttggggcg gagtgatttt
    22381 gaaatagtaa tcgcagcctg aatacatagc gtgtgcgcca ctggcgcgcg ctcattgtgt
    22441 tatcaggaat ccttgaaatc atgtacgcag cttgcatcac acatcctagc tatacataaa
    22501 caacaaggcc gtctgaattt tcagacggcc ttttccgttt acaactttac tacttattca
    22561 ataactacac gtgcattcag aggctgtacg gggcggttgt tttccgaacc gacggcgcgg
    22621 gtgaattttt ccgcttgcgc ttggtcgatg tggtcataag ttttcaacac caaccatgat
    22681 acgccctctg tgcacggcgg cgtggtcagc gaaccggcaa agcggtagta tttcaaccgt
    22741 ttcggcagta gggtggatgc gtcgaacggt tggttgagtt tcacttttcc tgcggtcatc
    22801 ggcatgacgt tccagatgga agacaggcgg ccgttggttt tgccggcttc atacagcacg
    22861 gctaatacta aaggctgttt gttttcgtct aagtggacga agtgagcttc catcgggaaa
    22921 gtgcggcctt tgatttggtt ttcgctcggc acgtggaagt ggaactgttt cagggtatag
    22981 gtgcggccgt tcacggtcag ggtattgccg ccttcgggat aattgacctg aatggtgtgg
    23041 ccgttgtttt ccacgtcaac catactcggt ttgtaattga ctttgatggc gggcagtttg
    23101 ccggaaacgg tttcggtaat gtttaccgga gattggtttt tgccggtgga gcacaaacgg
    23161 aattcttctg acagattgcc ccagctttcg ggagagtcgt gtccggtata gccccaatgg
    23221 gtgtgattgc cgtgtgcggc ggcggagaaa gcggtacagg ccaacagcag gacagcggtc
    23281 aggcggggta aagtgcgggg gaaacgaggc attgtaagat cctttctatt aaggagtaga
    23341 aaa
//
LOCUS       DAWTHD010000018        21603 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWTHD010000018 DAWTHD010000000
VERSION     DAWTHD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21603
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.018"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..1614
                     /locus_tag="WM704_000580"
     CDS             <1..1614
                     /locus_tag="WM704_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HFA9340064.1"
                     /translation="LLLMAFAWLWPGVFSHDLWNPAEPAVYTAVEALAGSPTPLVAHL
                     FGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFAGVFFAVIGLTSCGFAGFNFLG
                     RHHGRSVVLIHIGCIGLIPVAHFLNPAAAAFAAAGLVLHGYSLARRRVIAASFLLGTG
                     WTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLTAVASLAFALPLMTVYPLLLAK
                     TQPALFAQWLNYHVFGTFGGVRHIQTAFSLFYYLKNLLWFALPALPLAVWTVCRTRLF
                     STDWGILGIVWMLAVLVLLAFNPQRFQDNLVWLLPPLALFGAAQLDSLRRGAAAFVNW
                     FGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSPYYVPDIDPIPMAVAVLFTPLW
                     LWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWLDAAKSHAPVVRSMEASFSPEL
                     KRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDVRCRYRIVRLPQNADAPQGWQT
                     VWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            1672..1776
                     /locus_tag="WM704_000581"
                     /pseudo
     CDS             1672..1776
                     /locus_tag="WM704_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1821..2426
                     /locus_tag="WM704_000582"
     CDS             1821..2426
                     /locus_tag="WM704_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFA9340065.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            2498..2890
                     /locus_tag="WM704_000583"
     CDS             2498..2890
                     /locus_tag="WM704_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFA9340066.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            2895..3041
                     /locus_tag="WM704_000584"
     CDS             2895..3041
                     /locus_tag="WM704_000584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340067.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(3089..3535)
                     /locus_tag="WM704_000585"
     CDS             complement(3089..3535)
                     /locus_tag="WM704_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFA9340068.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEVGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(3667..3879)
                     /locus_tag="WM704_000586"
     CDS             complement(3667..3879)
                     /locus_tag="WM704_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFA9340069.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4168..6018)
                     /locus_tag="WM704_000587"
     CDS             complement(4168..6018)
                     /locus_tag="WM704_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9340070.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSGAGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            6395..7132
                     /locus_tag="WM704_000588"
     CDS             6395..7132
                     /locus_tag="WM704_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFA9340071.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            7134..7820
                     /locus_tag="WM704_000589"
     CDS             7134..7820
                     /locus_tag="WM704_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9340072.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            7979..8845
                     /locus_tag="WM704_000590"
     CDS             7979..8845
                     /locus_tag="WM704_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340073.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(8908..9477)
                     /locus_tag="WM704_000591"
     CDS             complement(8908..9477)
                     /locus_tag="WM704_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFA9340074.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            9635..10495
                     /locus_tag="WM704_000592"
     CDS             9635..10495
                     /locus_tag="WM704_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFA9340075.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            10565..10807
                     /locus_tag="WM704_000593"
     CDS             10565..10807
                     /locus_tag="WM704_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340076.1"
                     /translation="MTFFLLRVRIVFLFSFLIFEEIMNTRINFTATVGKRVSQCYVTS
                     VISTIGVTTSDAICSGGGTNKAKSQCNALLKAAGRC"
     gene            11170..11523
                     /locus_tag="WM704_000594"
     CDS             11170..11523
                     /locus_tag="WM704_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFA9340077.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            11513..12379
                     /gene="atpB"
                     /locus_tag="WM704_000595"
     CDS             11513..12379
                     /gene="atpB"
                     /locus_tag="WM704_000595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFA9340078.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            12436..12672
                     /gene="atpE"
                     /locus_tag="WM704_000596"
     CDS             12436..12672
                     /gene="atpE"
                     /locus_tag="WM704_000596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFA9340079.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            12742..13212
                     /locus_tag="WM704_000597"
     CDS             12742..13212
                     /locus_tag="WM704_000597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFA9340080.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            13217..13750
                     /locus_tag="WM704_000598"
     CDS             13217..13750
                     /locus_tag="WM704_000598"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFA9340081.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ALLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            13761..15308
                     /locus_tag="WM704_000599"
     CDS             13761..15308
                     /locus_tag="WM704_000599"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFA9340082.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            15333..16208
                     /gene="atpG"
                     /locus_tag="WM704_000600"
     CDS             15333..16208
                     /gene="atpG"
                     /locus_tag="WM704_000600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFA9340083.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDKIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKPPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            16246..17643
                     /gene="atpD"
                     /locus_tag="WM704_000601"
     CDS             16246..17643
                     /gene="atpD"
                     /locus_tag="WM704_000601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFA9340084.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            17654..18076
                     /locus_tag="WM704_000602"
     CDS             17654..18076
                     /locus_tag="WM704_000602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFA9340085.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            18248..19144
                     /gene="glyQ"
                     /locus_tag="WM704_000603"
     CDS             18248..19144
                     /gene="glyQ"
                     /locus_tag="WM704_000603"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFA9340086.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            19261..21324
                     /locus_tag="WM704_000604"
     CDS             19261..21324
                     /locus_tag="WM704_000604"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFA9340087.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEIIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAMPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAGRAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            21388..>21603
                     /locus_tag="WM704_000605"
     CDS             21388..>21603
                     /locus_tag="WM704_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9340088.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNSGLI"
BASE COUNT         4990 a         5802 c         5726 g         5085 t
ORIGIN      
        1 ctgctgttga tggcgtttgc ctggctgtgg cccggcgtgt tttcccacga tttgtggaat
       61 cctgccgaac ctgccgtcta taccgccgtc gaagcactgg caggcagccc cacccccttg
      121 gttgcccatc tgttcggtca aaccgatttc ggcataccgc ccgtgtatct ttgggttgcc
      181 gccgcattca aacatttgct gtcgccgtgg gcagccgacc cgtatgatgc cgcacgcttt
      241 gcaggcgtat tttttgccgt tatcggactg acttcttgcg gctttgccgg tttcaacttt
      301 ttgggcagac accacgggcg cagcgttgtt ttaatccata tcggctgtat cgggctgatt
      361 ccggttgccc atttcctcaa tcccgccgcc gccgcctttg ccgccgccgg actggtgctg
      421 cacggctact cgctggcacg ccggcgcgtg attgccgcct ctttcctgct cggtacgggt
      481 tggacgttga tgtcgctggc ggcagcttat ccggcggcgt ttgcgctgat gctgcccctg
      541 cccgtgctga tgtttttccg tccgtggcaa agcaggcgtt tgatgttgac ggcagtcgcc
      601 tcgcttgcct ttgccctgcc gcttatgacc gtttacccgc tgctcttggc aaaaacgcag
      661 cccgcgctgt ttgcgcaatg gctcaactat cacgttttcg gtacgttcgg cggcgtgcgg
      721 cacattcaga cggcattcag tttgttttac tatctgaaaa atctgctttg gttcgcactg
      781 cccgcgctgc cgctggcggt ttggacggtt tgccgcacac gcctgttttc gaccgactgg
      841 gggattttgg gcattgtctg gatgcttgcc gttttggtgc tgctcgcctt taatccgcag
      901 cgttttcaag acaacctcgt ctggctgctg ccgccgcttg ccctgttcgg cgcggcgcaa
      961 ctggacagcc tgaggcgcgg cgcggcggct tttgtcaact ggttcggcat tatggcgttc
     1021 gggctgtttg ccgtgttcct gtggacgggc tttttcgcca tgaattacgg ctggcccgcc
     1081 aagcttgccg aacgcgccgc ctacttcagc ccgtattacg ttcccgacat cgatcccatt
     1141 ccgatggcgg ttgccgtact gttcacaccc ttgtggctgt gggcgattac ccggaaaaac
     1201 atacgcggca ggcaggcggt taccaactgg gcggcaggcg ttaccctgac ctgggctttg
     1261 ctgatgacgc tgttcctgcc gtggctggac gcggcgaaaa gccacgcgcc cgtcgtccgg
     1321 agtatggagg catcgttttc cccggaatta aaacgggagc tttcagacgg catcgagtgt
     1381 atcggcatag gcggcggcga cctgcacacg cggattgttt ggacgcagta cggcacattg
     1441 ccgcaccgcg tcggcgatgt ccgttgccgc taccgtatcg tccgcctgcc ccaaaacgcg
     1501 gatgcgccgc aaggctggca gacggtctgg cagggtgcgc gcccgcgcaa caaagacagt
     1561 aagtttgcac tgatacggaa aatcggggaa aatatattaa aaacaacaga ttgagccgaa
     1621 tttctggatt aagtgccgga aatcgcgtat aattgcgcga ttaaaccttt atatagtgga
     1681 ttaaatttaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc
     1741 gaggcaacgc cgtaccggtt taaatttaat ccactataaa tcagccgttt tgcaggcatc
     1801 acacaggagc gacaattatt atgatgaccc tctattccgg cattacctgc cccttcagcc
     1861 accgctgccg cttcgttttg tacgaaaaag gtatggattt tgaaatcaaa gacatcgata
     1921 tttacaacaa acccgaagac ctcgccgtca tgaacccgta caaccaagtc cccgtgctgg
     1981 tcgagcgcga tttggtgctg cacgagtcca acatcatcaa cgaatacatc gacgaacgct
     2041 tcccccatcc gcagcttatg cccggcgatc ccgttatgcg cggccggggg cggctggtgc
     2101 tgtaccgtat ggaaaaagaa ttgttcaacc acgtccaagt tttggaaaac cccgccgccg
     2161 ccaacaagga acaggcaaaa gcgcgcgaag ccatcggcaa cggcctgacg atgctttccc
     2221 cttcgttcag caaaagcaaa tacatcctcg gcgaagattt ttctatgatt gatgttgccc
     2281 tggccccgct gttgtggcgg ctcgaccatt acgatgtcaa actgggcaaa agtgccgcgc
     2341 cgctgctcaa atacgccgag cgtatcttcc agcgcgaagc ctttatcgaa gcactgacac
     2401 ccgccgaaaa agccatgcgc aaataagtcc gaaatgcttg caaaacccgc cgttttgcag
     2461 gcattttcac attcaggcac accacacgga acccatcatg cccacctcaa ccaaacccta
     2521 catcctccgc gcactttgcg aatggtgcag cgacaacagc ctcacgccgc acatccttgt
     2581 ctgggtaaac gagcatacgc gcgtccccat gcagtacgtc cgcgacaacg aaattatgct
     2641 caacatcggc gcaaccgcca cgcaaaacct tcgaatcgac aacgattgga tcagcttttc
     2701 cgcccgtttc ggcggacaag cgcacgacat atggatacct gtcggacacg tcctcagcct
     2761 tttcgcacgg gagaccggag aaggtatggg gtttgaattg gaggagtacc gccccgacac
     2821 gccgtctgaa aacacctctg ccgaaaccgc gccccggccc gccaaaaaag gtttgaaatt
     2881 ggtcaaataa atctatgccg tctgaacgga atcgtgtttc agacggcatt ttgtccgatg
     2941 gggcgcaaac ggaatccgtt tatcggcaaa acctgtttcg gcgtatcgaa accgtgttgc
     3001 ccctgccgat tcgatattgg gacattgaaa atgccgtctg aacctgcgat acgggcttca
     3061 gacggcgttt tgtccgatat tcgggcaatc aggcggtcag tacggttttc aagattttgt
     3121 tgacttcgcc catatcggct ttgccggcga gacgggtttt caatacgacc atcactttgc
     3181 ccatatccgc catacctgcc gcgccggttt cggcaacggc tgcttcgacg gcggtgcgga
     3241 tttcgccggc ggagagcatt tgcggcaggt agcggtgcag cacgtcgatt tcggcgtttt
     3301 ctttgtctgc caaatcctga cggccgactt cagtgtagat tttcgcgctg tctttgcgct
     3361 gtttgaccat tttggtcagg atggcggtga ttttggcatc gtcggcttcg gtgcgttcgt
     3421 ctacttcaaa ctgtttgacg gcggcattga tgaggcggat ggtgccgagg gaaacttgat
     3481 ctttggcgcg catcgcggtt ttcatatctt cggtaaggcg ggttttcagg ctcatgatgt
     3541 cctcgctggg atgtcggatg gaaacggcgg gtttcgatgc cgtctgaaaa gcaagacaca
     3601 ccgcagagca ggtcttgcgg tgtgtcttca tatcacaggg ctgacctgta atctgtactt
     3661 gaacgtttag tacattttgg gcggcagctg ttggctgcgc aggcgttttt gcaggcgttt
     3721 tacggctgcc gcttttttgc gtttgcgttc ggtagtcggt ttttcgtagg cttcgcgggc
     3781 gcgcagctcg gtcagcaggc cggttttttc tacggcgcgt ttgaaacggc gcatagcgac
     3841 ttcaaagggt tcgttctctt ttacgcggat tgcaggcatt ttatttcctt taataaattc
     3901 ggttgtttca tctgcccatc atatcggtgg aaagggtagg gcagacggtg ttgtaggttt
     3961 tccgtatcgc tgtgcctttc ggcggcggga tacgcgccgt gacggaaaca tcacctccta
     4021 acgggtcggc ttgtgccgtg tcaagctgct tgaaaaagta ggaaaaacaa ttttcggatt
     4081 gtcttatatt tatggttcgc cgtcaatgcc gttcgggata aaaatgccgt ctgaaagacc
     4141 gggcgggttt cagacggcat cggcacgtca gcgtgcggga acggtgccca tacgctgttt
     4201 gagcgggatg tgcggcgcgc cgaagaggga ggcgtagtag gcggcattgg tcatcacttt
     4261 tttgacatag tcgcgcgttt cggaaaacgg gatggtttcg gcatataccg cgccttcgag
     4321 gggtgtgtcc gcctgccatc ggcgcgctct gccggggccg gcgttatagc ctgcggtggc
     4381 gaggatttcg ttgttttgca ggcggcgttt ggtgtccgcc atataccacg tccccatacg
     4441 gatattgccg tcggcggtgt agagctgggc ggcatccata ccgattttgc cggcgatttc
     4501 gcgcgcggtg gcgggcataa cctgcatcag cccctgcgcg cccgcgccgg attgcgcgcc
     4561 cataacgaaa cggctttcct gacgaatcag gccgtaaacc caagccggat cgacattaac
     4621 attttgcgcg tggcggatta cggtgtcttt aaacggcgaa atgtagcgca atgtgtagtt
     4681 gagtttgcgg tcggtgcgtt ccgcgctgtt gaccgccata tcgtaaaaac cgtggtcgaa
     4741 cgcggtttgc gcggcggtca gcagcttgtc ttcgtcaaag ccgcgtgtgg caaaacgcca
     4801 ttccgcctga gcctgacggc gcattttcgc atcgccggcg gttcggctgt ttcggaacag
     4861 caccagcgcg cgcttaatcg cgccgtcttc cgccatacgg aggacactgt ttttgccggc
     4921 atcgggcaca ttgttgcgcg tatcgatttt gcgccccaat tcttcccctg ccagtacggc
     4981 ataaaaattc ctgcccgtcg ctgccgcctg tttgtaaagt ttttccgctt cttgcgtgtt
     5041 gcccgttgcg gcgcggctgc gtgccagcca gtagagccag gtcgggcttt tttgcagttt
     5101 ttcgggcata tgcgagatca cggaggccag ctcgtcccaa cgtcgggcgc gcaaggcggc
     5161 gcgggcgtac cactcgattt ggtcgtcggt cagttggcgg cggtcggcaa ccttgccgta
     5221 atagtccaag gcggcaggca cattgaggct ttgcgactga taatgcccca atacgcccca
     5281 cgcgaaactg cgttgttcgg ggcttaaacc gctttccatt tcggacagca gggcggcggc
     5341 attcggcgat ttgcgtgctt ctttgccgat gacgttcaac agggcatatt cgcgcgaacc
     5401 ttgtgtaccg ccgtcaaacg ggctgcccaa tgcggcggca aggttgcgcg cgtccgtcgt
     5461 ttggcggccg gccagcagtc cgcgcacgcg cctccaggca tcgttgccgt ccaacaagcc
     5521 ggatgcggct gcctgttcca acagtttggt gcagcccgaa ggcagtttgc ccgtattttt
     5581 gaccagttcg gcggcacgcg tatagtcgtt gcggctcgaa tcggcgtagc attcgacttc
     5641 ttgggcgcgc ccttcaggtt tgagtttggc atattcctgt gcaaacagcg tccactgtct
     5701 gcgcgcgccc aaagacttca gccactcgtt gcggacattt tccgccatcg cgctgtcgcc
     5761 ggcgttttcc aaataggcgg cggctgccgc atcgttgttc tgtttcactg catccagtgc
     5821 ggacgggtag ccgccgtaat ctgccagcgt ttttccttcc ggttcggcag ggcgggtggg
     5881 aacgcttgcc gaaaggtcgg cagtttctat attgtctgcc ggggtcttgc cggctggcag
     5941 tgtgtttgtc gaagaacacg cggcaagcac cagggccgcc agcagcggca gggaatgctt
     6001 catagagggt aggtacatcg gatttcctta agaatcggaa ccctgaacgg tcagggttgg
     6061 aaaagacaaa atgccgtctg aacaggcgtt tgcccgaatt atatgccgaa actgcaccgt
     6121 ctttggaatg tttccgacat aatttatatt tttcaattat ttgccgtttg ggtgcgaacc
     6181 gctgcctttg cccgtttcag acggcattgt ccgaaatggt tgcccgcttc ctgctttatt
     6241 gacaaaaaaa cgctttcccg ataatatcct acgaaaatta acctgccgat ttgacacagc
     6301 ttgcgggcat aacagctaaa gcgttccgac aatttcagct ttatcttccg cgcccgttgt
     6361 gtccgacatc gggctttgtt gtatgggaaa gacaatgatt attttggaca aggtttccaa
     6421 gcattaccaa acgcgcgaca aaacccgttt tgccgccgtc gagccgacca gcctcgaaat
     6481 tcaagacggc gaaatcttcg ggctgatggg ctattccggc gcgggcaaat ccactttgtt
     6541 gcgcctgatc aacctgttgg aacgccccga cacgggcaaa gtcaacgtct gcggacaaga
     6601 actgaccgcg ctcgatgccg ccgcattgcg tcaggcgcgg cagaatatcg gcatggtgtt
     6661 tcagcagttc aaccttttaa gcaaccgcac cgtcgccggc aacgttgcct ttccactgga
     6721 aatcgccgga tggccgtctg aaaaaatcaa agcgcgcgtt gccgagtgcc tcgaaatcgt
     6781 cggcttgacc gaacgcgccg gccactaccc cgcccagctt tcaggcgggc agaaacaacg
     6841 cgtcggaatc gcccgcgcgc tcgcgcccaa gccgcaagtc atcctcgccg acgaacccac
     6901 ttccgccctc gaccccgcca ccacgcgcag tgtcttggaa tgtttggaag acatcaacaa
     6961 acgcttcaac gttaccatcg tcatcgtaac ccacgaaatg agcgtcatca gacgattgtg
     7021 cgaccgcgcc gccctgttgg ataaagggaa agtggtggaa atcgtcgaag tacgcggcaa
     7081 ccaaatccac gctcaatccg aaatcgggcg cgaactgatt cgggaggact gatatggcag
     7141 acttaacatt cgaacaagcc gtttccacca tcgtcggcat gaaagacgaa atcgtccgcg
     7201 ccttgggcga aactttcgtg atggtcggct tgtccaccac attcgccgtc atcttcggca
     7261 cgctcttggg cgtgttgctc ttcgtaactt ccagccgcca actgcattac aacaagccgg
     7321 taaacttcct gctcgacaac ctcgtcaacc tcatgcgcgc cttccccttc gtcatcctga
     7381 tgattgcgat gatacccgcc acacgcgcca tcgtcggcag caccatcggc ccggttgccg
     7441 cctcgctggt gttgagcgtg tcgggattgt tttattttgc ccgactggtg gaacaaaacc
     7501 tgcgcgaagt cccaaaaggt gtgattgaag ccgccgccgc gatgggtgcg ccgccgattg
     7561 ccatcgtcct caaagtcctc ttgaacgaag cgcgcgcggg catggtttcc agcattaccg
     7621 tgcttgccat cgggcttttg tcatacagcg cggcggcagg catgataggc ggcggcggct
     7681 tgggcgacct cgccatccgc tacggctact accgctacca aaccgaagtc atcgtcttca
     7741 tcgtcgccct cctcgtgctg ctggtcatcc tgattcaaag caccggcaac gcgttggcgc
     7801 ggaaactcga caaacgttaa acccgaatgc cgtctgaacg ccaaaaaccc cgccgctatc
     7861 cgaaaaatgc tataaaatcc ccctgttcgc ggcaaatgcc gtctgaacgc cgaatccgga
     7921 cggcaggact ccctgcctgt catttttgtt tgaaaccgcc acaacatcag gagaaaatat
     7981 gaaaaccttc ttcaaaaccc tttccgccgc cgcactcgcg ctcatcctcg cagcctgcgg
     8041 cggtcaaaaa gacagcgcgc ccgcagcctc tgccgccgcc ccttctgccg ataacggcgc
     8101 ggcgaaaaaa gaaatcgtct tcggcacgac cgtcggcgac ttcggcgata tggtcaaaga
     8161 acaaatccaa gccgagctgg agaaaaaagg ctacaccgtc aaattggtcg aatttaccga
     8221 ctatgtgcgc ccgaatctgg cattggcgga gggcgagttg gacatcaacg tcttccaaca
     8281 caaaccctat cttgacgatt tcaaaaaaga acacaacctg gacatcaccg aagccttcca
     8341 agtgccgacc gcgcctttgg gactgtatcc gggcaaactg aaatcgctgg aagaagtcaa
     8401 agacggcagc accgtatccg cgcccaacga cccgtccaac ttcgcacgcg ccttggtgat
     8461 gctgaacgaa ctgggttgga tcaaactcaa agacggcatc aatccgctga ccgcatccaa
     8521 agccgacatc gcggaaaacc tgaaaaacat caaaatcgtc gagcttgaag ccgcacaact
     8581 gccgcgcagc cgcgccgacg tggattttgc cgtcgtcaac ggcaactacg ccataagcag
     8641 cggcatgaag ctgaccgaag ccctgttcca agagccgagc tttgcctatg tcaactggtc
     8701 tgccgtcaaa accgccgaca aagacagcca atggcttaaa gacgtaaccg aggcctataa
     8761 ctccgacgcg ttcaaagcct acgcgcacaa acgcttcgag ggctacaaat accctgccgc
     8821 atggaatgaa ggcgcagcca aataaggcag tcgtataaaa tgatgccgtc tgaaccgcat
     8881 tccgtgttca gacggcattt ttgtccttta atccgccatt ccctgccatt ccgccaaatc
     8941 cggcgtatcg attccgaaca gcgacagcgt gtgtgcgata ctgtgcgcca ctatgtcgtc
     9001 cgccgtctgc ggcttgcggt acatcgcagg aacaggggga aacaccacgc cgcccatttc
     9061 cgttacccgc ttcatattgt ccaaatgggc aaggttcagc ggcgtttcgc gcaccatcag
     9121 caccagccgc cgcctttcct tcaaaaccac atccgccgca cgcgtcaaga gattgtcgcc
     9181 gaagccgtgc gcgacagagg caagcgtccg catcgaacag ggtgcgacca gcatcccgtc
     9241 cgttttaaac gtaccgctgg caatgcacgc cccgatattg ccgatcggat ggacgaaatc
     9301 agccaaggca tatacttcgt ctttcgtata atccgtttcc gaagcgcgcg ccatctccgc
     9361 gcctttcgat accacaaggt gcgtttcgac atcttgcgcg cgcaaaagtt ccaaagcctt
     9421 cacgccgtat tggaaaccgc tcgccccgct gatgccgatt atcaaacgcc gtaccatcat
     9481 ccgcctttcc cataaaaccg cctgcaacgg caaaccggct attatagtga aaaaacagaa
     9541 atccgataaa cacggataca aattgtcggc aacacccaat atccgataaa atacccgatt
     9601 taacatccta tctgaatagg cacgggaggg cggtatggca aaagtaaaag gcggattggg
     9661 gcgcggcttg gattcgctgc tcgccaacgg tgcggacaac agcagcggcg accggctgac
     9721 tgcggttgcg gttaaagata tccggcccgg ccgctatcag gcgcgcgtcc aaatcgatga
     9781 cgaagccttg caggaactgg cagattcgat taaggcgcaa ggcgtgatac agcccgtcat
     9841 cgtgcgcgaa cacggactgt cccgatacga actgattgca ggcgaacgcc gttggcgcgc
     9901 cgctcagatt gccggcctgt ccgaaatccc cgccgttatc aaaaccatca gcgacgaaac
     9961 cgcattggca atgggtttga tcgaaaacct ccagcgcgaa aacctcaacc ccatcgaaga
    10021 agcacaaggc ttgaaacgcc ttgccgacga gttcgggctg acccacgaaa ccatcgccca
    10081 agccgtcggc aaaagccgaa gcgcgatttc caacagcctg cgccttttaa gcctgcccga
    10141 atccgtgcag gaaatgcttt accaacgccg cctcgaaatg gggcacgccc gcgcactgct
    10201 gaccctgccc gtcgtcgaac agctcgaatt ggcgcaaaag gccgtcaaaa acggctggtc
    10261 ggtgcgcgaa gtcgaacgcc gcagccaggc cgcccttcaa aacaaacgtc ccgagccaaa
    10321 aaaaactgcc gccgccgaca tcggccgcct gaatgatttg ctgactgaaa aattgggtgt
    10381 caacgccgaa atcaagaccg ccaaccataa aaaaggcaaa atcatcctgc atttcgacac
    10441 gcccgaaacc ttcgaccata tcttgaagca gttgggcata gattaccggc cttaattttg
    10501 cgggatatgc cgtttgaaat atagagaata gctttccaga ttttaagtgg gaaatataat
    10561 tctattgaca ttttttctgc ttcgcgtaag aatcgttttc ctgttttcat ttttaatttt
    10621 cgaggaaatt atgaacacac gcatcaactt taccgcaact gtgggtaagc gcgtgagcca
    10681 atgctatgtt accagtgtaa tcagcacaat cggcgttacc acttccgatg caatttgttc
    10741 gggaggcgga acgaacaaag ccaaaagtca atgcaatgct ttgcttaaag cggcaggccg
    10801 ctgctaatcc tttattcgga aaaggtcgtc tgaaaatatt ttcagacgac cttttattta
    10861 ttgagcaaac tccccaaact gccgacaatc cgaccgataa actactacaa tttcgcgttg
    10921 attttttatt atttccacgt aaagcaaaca aatcacgtaa gaacattaaa agtgtaaaga
    10981 aattgcggac aaactcctta atatcctgta atcatatgta atatgtgtta gaatagcgga
    11041 gttggatttt ggggagtcgc tcgtctgtat cggcggcgag tttcacaaag attaacatat
    11101 tgcttgacgt aataaggctt ctaaattata ttagccgcgc cccaaacctg aggcggaaac
    11161 attcccttta tgaagcagat tatcatccta caatccgccg tattgtccat atgcgccgca
    11221 gttgcctttg ccgtatgggg ttttgccgga ttcctttcgg cggtcggggg cggtttgtcc
    11281 tacctgcttc ccacgtttgt tgcagtttta cttctaaaac ttttcagggg aaaccccttc
    11341 ctgcaaagca ggatgttcgt cttcggagag attttaaaag tagtgctgtc gctgttgtcc
    11401 atgcttgccg tatttgcaat atggcatcaa tcgctggtgt tcgtcccgtt tttgatgggg
    11461 ctgctcggtg tcagccatct ggttttttta gtattgttga gagtgaaaga ttatggcagg
    11521 tgaaaccatt accgctgccg actacatcaa gcaccacttg cagagcttga ccagtttgtc
    11581 ggatgttact cagggtcagg gactgaaaaa cattgctgat ttttcgttta ttaaccttga
    11641 tgccgttttt tttgccgtcc tgttgggcgt aatcggcagc ttcctgttgt ggcgcggtgc
    11701 aaaaaaagca acggcaggcg ttcccggacg tttccaggcc gccgtggaaa tcttgttcga
    11761 gtttgtggac gatatgtgta agagcatcat tcacagcgaa aagtctagaa aagccgtcgc
    11821 gccgttgggt ctgacgctgt ttgtttggat tttcctgatg aacgcgatgg atatgctgcc
    11881 ggtcgatttg ctgccgatgg tatggcaggg cattaccggc aaccatcacg ccctgctgcg
    11941 catcgtaccg actgccgatt tgaacaccgc tttggcactc gctgtcggcg tgttgctgat
    12001 ttgtatctat tacaacatca aaatcaaagg attgggcggc tggtttcacg agttgttcag
    12061 tgcgccgttc ggtgcaaaac tcgcacctgc gaactttctg ttgaacttgg tcgagtttct
    12121 ttctaaaacc gtatcccacg gtatgcggtt gttcggcaat atgtatgcgg gcgagctggt
    12181 attcctgctg atagccttgc ttggcggtgc ttgggcggct tccggcagcg ttgaagtcat
    12241 ggatccgatt ctgtttgtat tccacattat tgccggtttg gcatgggcga ttttccatat
    12301 tttggtgatt accctgcagg catttatttt catggcgttg gcgttcgtct atatcgggca
    12361 ggcgcacgat gcccactgat tttcccaagt agtttgtttt tgtagtttaa cctttgtttc
    12421 tttaaggagt ttaaaatggg tttgattgct atcgcatgtg gtttgatcgt tgcattgggt
    12481 gcattgggtg catctatcgg tatcgcaatg gtcggttcta aatatttgga gtcttctgct
    12541 cgccaacctg aactgattgg tccgctgcaa accaaactgt tcctgattgc cggtctgatt
    12601 gatgccgcat tcttgatcgg tgtcgccatt gcactactgt tcgccttcgt caacccgttt
    12661 gcaggtgcat aatcaggacg gattcgtccg ttcgtacagg tgcagcctgt ttgattaacc
    12721 ccaatacgaa ggttaagtaa cgtgaatatc aatgcaacat tattcgctca aatcatcgtc
    12781 tttttcggtt tggtatggtt taccatgaaa tttgtgtggc cgccgattgc aaaagctttg
    12841 gatgagcgtg ccgcaaaaat cgccgagggc ttggctgccg ccgagcgtgg taaaagcgat
    12901 ttcgagcagg ctgaaaaaaa ggttgcagaa cttttggcag aagggcgtaa tcaggtttcc
    12961 gaaatggttg ccaacgccga aaaacgtgcc gccaaaattg tcgaagaagc caaagaacag
    13021 gcttcttccg aggcggcgcg cattgcagct caggcaaagg ccgatgtgga gcaggaattg
    13081 ttccgcgcac gcgaatccct gcgcgatcag gttgccgtgt tggctgtcaa aggtgccgaa
    13141 tctattttgc gcagcgaagt cgatgcttcc aaacacgcaa aactgctcga taccctgaaa
    13201 caggagttgt aatcttatgg cagagtttgc aacgatcgcc agaccttatg caaaggcatt
    13261 gttcggtctg gctcaggaaa aaaaccaaat tgagtcttgg ttgggcggac tggaaaaact
    13321 tgcggcggtt gttcaggaag ggaaggtggc tttattgatt gaccgtcctg aaacgaatgc
    13381 ttcagaaaaa gcagatatcc tcatcgattt ggtcggtttg aaagacaagg agttaaaaaa
    13441 ctttgttatc gtcttggccg ggcagaaacg tttgtcgata ttgccggaag tgtatgctca
    13501 atatcaagac ttgaccttat cattcaacca tatcaaatct gccgtcattt acagtgccta
    13561 tccgttgacc gacaaacagg tcggcgagtt ggcgcaaatg ctgaataagc gtttcgacag
    13621 cgagctgaaa atctctgtcg aaatcgaacc ggagctgatt ggcggcataa aagttgaagt
    13681 gggtgatcag gttttggatt tgtctgtaca aggcaaactg agtgctttgt acacgactat
    13741 gacgaattag gagagttttc atgcagctta atcctgctga aattagcgat ctgattaaag
    13801 ccaagatcga aaatctgtct gtaaatgccg aagtgagtac ccgtggtacg gtgatttcgg
    13861 taactgacgg tatcgttcgc atccatggtt tgtcagatgc aatgcaaggt gagatgctcg
    13921 aattcccggg taacacttta ggcttggcga tgaacctgga gcgcgactcc gtcggtgccg
    13981 tagtgttggg cgagtacgaa catattaaag aaggcgacac ggttacctgt accggccgta
    14041 ttttagaagt gccggtcgga cgcgaattgg tcggacgcgt cgtcgatgca ttgggtcggc
    14101 ctatcgacgg caaaggcccg attaatacaa ctttgaccgc ccctgtcgaa aaaatcgcac
    14161 cgggcgtgat tgcacgcaaa tcggttgatc agccgatgca aaccggtctg aaggcgattg
    14221 actctatgat tcctgtcggt cgcggtcagc gtgagttgat tattggagac cgtcagacag
    14281 gtaaaacggc cgtggcattg gatgccatcg tcaaccaaaa aggtacgggt gttatctgta
    14341 tctatgtcgc tatcggtcaa aaagcatctt ctattgccaa cgtggtccgc aaattggaag
    14401 agcatggcgc gatggaacac acgattgtgg ttgctgcaac tgcatctgaa gcggcggcat
    14461 tgcaatatat cgcaccttat tcaggttgta cgatgggtga attcttccgc gatcgtggcg
    14521 aagatgcctt gattgtttat gacgatttgt ccaaacaggc tgtggcttac cgtcaaattt
    14581 ccctgctttt gcgccgtccg cccggccgcg aagcctatcc cggcgatgtg ttctacctgc
    14641 actcccgtct gttggaacgt gcggcacgtg tcaatgaaca cgaagtggaa aaattgacca
    14701 acggcgaagt aaaaggcaaa accggttctc tgaccgcgtt gccgattatc gaaacccaag
    14761 cgggcgacgt atctgctttc gttccgacta acgtcatttc gattaccgac ggtcagattt
    14821 tcttggaaac cgacctcttc aacgccggta tccgtcctgc aatcaatgcc ggtatttccg
    14881 tatcccgcgt aggcggtgct gcacaaacca aagtgattaa aaagctgggt ggcggtatcc
    14941 gtttggcgtt ggcacaatat cgtgaattgg cggcgttctc gcaatttgca tccgatttgg
    15001 atgaagctac gcgcaaacag ttggagcatg gcgaagtcgt aaccgaactg atgaaacaga
    15061 aacagttcag tacgttgaat acggctgaaa tggctttgac cctttgggca atcaacaacg
    15121 gttcgtattc tgatgttccg gttgccaaag ctctagcttt tgaatctgaa tttttgagct
    15181 ttgtccgtac ccaacatccg gaagttttgg aagccgtcaa tgcttcaggt gcaatgtccg
    15241 acgagagcga gaaaacgctt gaagcagcca tgaaatcctt caaatcttct tacgcctatc
    15301 aggcataagg cttgaagtga aaggagtctg aaatggcagt aggaaaagag attctcacca
    15361 aaatccgcag tgttcagaat acccaaaaga tcactaaagc gatgcaaatg gtgtcaacct
    15421 ctaaaatgcg gaagactcag gaacggatgc gtttggcgcg tccgtatgcc gaaaaagtgc
    15481 gtatggtgat gagccatctt gcgcaaacca ataccgatca tggtattccg ttactggaat
    15541 ctcatcggga aatcagacgt gtcggtttta ttttgattac gtctgataag ggtttgtgtg
    15601 gcggtttgaa cgccaacgtg ctgaaaaagt ttttggcaca agttcaagag tatcggaatc
    15661 aaggtattga agaggaaatc gtatgccttg gcagtaaagg tctgatggcg tgtcagagca
    15721 ttggtctgaa tgtggttgcc agtgccgtaa atttgggcga taccccaaaa atggaaatgc
    15781 tgctcggacc tttgacagaa cttttccaac ggtatgagaa acatgaaatt gacaaaatcc
    15841 atctggtgta ttcgggtttt gtcaatacca tgcgtcaaga accgaggatg gaagtattgc
    15901 tgcctatcgg tgagaacgtg attggcgatt cagctcccaa accaccgttc agctgggaat
    15961 accgctacga accgactgca cttgcagtgt tggaatatct ggttcgccgc tatttagagt
    16021 ctgtggttta tcaggcgttg agcgacaata tggcatccga acaggcagcc cgcatggttg
    16081 ccatgaaagc tgcaacagac aatgcaggca atgccatcaa agagttgcgt ctggtatata
    16141 acaaatcgcg tcaagctgcg attaccacgg aattgtcaga aattgtagca ggtgcggcgg
    16201 ccgtctgatg ccgtctgaat cctgtacgga aattaggata cgataatgag ccaaggcaaa
    16261 atcgtacaaa ttatcggtgc ggttgttgac gtggaatttc cacgcgacat gattccgcgc
    16321 gtttacgacg ctttgaaatt agacgaaaac ggtctgactt tggaagtcca acagcttttg
    16381 ggtgatggcg tagtccgtac catcgctatg ggcagctcgg acggtttgaa acgcggcatg
    16441 actgtgagca atactggttc gcccattact gtgccggtag gtaaaggtac gttgggacgc
    16501 attgtcgatg tattgggaac tcctgttgac gaggcaggtc caattgatac cgacaagagt
    16561 cgtgccatcc accaagccgc tcctaagttt gacgaactgt cttccacaac cgaattgctc
    16621 gaaacgggca ttaaagtgat tgacttgctg tgtccgtttg ccaaaggcgg taaagtaggt
    16681 ctgttcggcg gtgccggtgt gggtaaaacc gtgaacatga tggaattgat caacaacatc
    16741 gccaaagcgc acagcggctt gtccgtgttc tcaggcgtgg gtgagcgtac ccgcgaaggt
    16801 aacgacttct accacgagat gaaagattcc aacgtattgg ataaagtagc catggtgtat
    16861 ggccaaatga acgaacctcc aggcaaccgt ctgcgcgttg ctttgaccgg tttgactatg
    16921 gctgaatact tccgtgacga aaaagacgaa aacggtaaag gtcgtgacgt attgttcttc
    16981 gttgacaaca tctaccgtta cactctggcc ggtaccgaag tatccgcact gttgggccgt
    17041 atgccttctg cagtgggtta ccaaccgaca ttggctgaag aaatgggtcg tttgcaagag
    17101 cgtattacct ctacccaaac cggttccatt acttccatcc aagccgtata tgtacctgcg
    17161 gatgacttga ctgacccgtc tccggcgaca actttcgccc acttggacgc gaccgtcgta
    17221 ttgagccgtg atattgcctc tttgggtatt tacccggcag ttgacccgct tgactctact
    17281 tcgcgccaat tggatccgat ggtattgggt caagagcact acgacgtagc gcgcggtgta
    17341 cagtctactc tgcaaaaata caaagaattg cgcgacatca tcgccattct gggtatggac
    17401 gaattgtctg acgaagacaa actggctgta atgcgtgccc gtaaaatcca acgcttcctg
    17461 tctcaaccgt tccacgttgc cgaagtgttt acaggttctc caggcaaata tgtcgccctg
    17521 cgcgatacca ttgccggctt caaagccatc ttgaacggcg aatacgatca tctgcccgaa
    17581 caggcattct atatggtcgg cagcatcgaa gaagcggttg agaaagcgaa aaccttaaac
    17641 tgaggaggtc ggcatgagca tcatgcaagt tgaggtggta agtggcgagc agaaaatcta
    17701 ttcaggcgag gcaacattta tcgttgttcc gactgtacag ggcgaactcg gtatttatcc
    17761 gcgacacgag ccgattatga gtttggtgcg gccgggggct ttgcgtttga ccgttccggg
    17821 cgaggataaa gaggttttgg ttgctgtttc cggcggtgtt ttggaagtac agcctgataa
    17881 agtaactgtc ttggcggatg ttgccgtccg cagtgcagag atggatcagg cacgtgcgga
    17941 agaggcgaaa aaagccgcag aagcgggtat ttcccaagct aaagacgata aggctttggc
    18001 ggaagcacat aaagcattgg ctgctgcgat tgcacagctc aaaactttgg actatatccg
    18061 ttcgcacaag aaataaactt gtttggtttg aaaaagcacg accgtttggc cgtgcttttt
    18121 attttccgga tttaaattaa aaaattatcc atcccctggt ttcaaatcag gctgtcttcg
    18181 agataaatct attcaagcgg ctatggaaac agtaaaataa aacctttatt ttatcgaaca
    18241 ataagccatg cttaccttcc aacaaatcat cttcaaactg caaaccttct gggcagacaa
    18301 aggctgcacc gtcatccaac ccttcgacat ggaagtcggt gccggcacat cccatcccgc
    18361 cacctgcctg cgcgcgctcg gccccgagcc ttggtttgcc gcctacgtcc aacccagccg
    18421 ccgccccaaa gacggccgct acggcgacaa ccccaaccgc ctgcaacact attaccaatt
    18481 ccaagtcgcc ctcaaacccg cccccgccaa tatccaagac ctctatctcg actccctgcg
    18541 cgaattgggc atcgatccca aagtccacga catccgcttt gtcgaagacg actgggaaaa
    18601 ccccaccctc ggtgcgtggg gtttgggctg ggaagtctgg ctcaacggca tggaagtaac
    18661 ccagtttacc tatttccaac aagtcggcgg tatcgattgc acgcccgtac tcggcgaaat
    18721 tacctacggc atcgaacgct tggcgatgta cctgcaaggc gtggaaaacg tctatgacct
    18781 cgtttgggca aaaacgccgg acggcaatac cgtaagctac ggcgacgtgt accatcaaaa
    18841 cgaagtcgag caatctacct acaacttcga atacagcgat gccgactggc tgctgcgcca
    18901 gttcaacgac tacgaagcgc aagccaaacg cctgttcgcc gaagaaaacg ccgaccttgc
    18961 cttacctgcc tacgagctgg tcctcaaagc ggggcatacg ttcaaccttt tagacgcacg
    19021 cggcgcgatt tccgtaaccg agcgggcaac ttatattgga cgtattcgtg cattgagccg
    19081 cgccgtggcg caaaaatata tcgaaagccg tgagaaactg ggcttcccgt tgattaaaaa
    19141 ataacgatac gtcgtctgaa gcaggttttc aggcgacctt tattgcgtat ccattaatac
    19201 aggccgtacg gcatatgcag gctttataag catggcctac ggacaaacag aaagtctcac
    19261 atgacaaccc aaaccctttt aatcgaactc cttaccgaag aactcccgcc aaaagccctg
    19321 aataatctgg gcaaccattt tgccgcttcc gttgccgaag gcttggaaaa agcgcaactg
    19381 gttgacggcg cggccgaatt tacggcttat gcttcgccgc gccgtttggc ggttcaagtc
    19441 aaaaacgtca aagctgttca ggccgatcag aaaatcgtga aaaaaggccc tgccgtggcg
    19501 aatgccgtaa aagacggtac gccgaccaag gctttggaag gttttgcgcg cggggcgggg
    19561 gcgaaaatcg aagacctgac catcatccac gacggcaggc aggacgtgta cgcctacgaa
    19621 tacgtccaaa ccggcagacc gttgggcgga cttttggaag acattatcaa tcaagctgtt
    19681 aagaagctgc cgattccgaa agtaatgcgt tggggcagca gcacgtttac cttcgtgcgc
    19741 cctgttcacg ggctgattgt gctgcacggc ggcgatgtcg tgaacgtcag cgttttgggc
    19801 ctgcaaagcg gcaatcaaac cttgggacac cgctttcttt ccgacggcga aatcatcatt
    19861 gaaaacgccg acagctacgc cgcacaaatg cgcgggcaag gcaaagtcgt cgcttcgttt
    19921 gccgggcgca aagccgcgat tcagacggca ttggaagggc aggcacgccg tctgaacgcg
    19981 accgttgccg ccgatgaagc cctgttggac gaagtaaccg cgctggtcga atggcctgtt
    20041 gttttggaag ccggttttga ggaacacttc ctcgccatgc ctcaagaatg cttgattttg
    20101 acgatgcagc aaaaccaaaa atacttcccg ctgctcgacc aaaacggcaa gctgatgaac
    20161 cgcttcctgc tggtttccaa cctgcaaacc gaagacccgt cgcacatcat ccgaggcaac
    20221 gaacgcgtat tgcgcgcgcg cctgtctgat gccgagttct tctacaaaca agaccaaaaa
    20281 gcgaccttgg aaagccgcct gcccaagctg gcgaacgtgg tgtaccacaa caaaatcggt
    20341 tcgcaggccg aacgcatcga acgcctgcaa agcatcgccg cccatatcgc caaagcattg
    20401 ggcgcggatg ccgcggcagc cgggcgcgcc gcgcgtcttg ccaaagccga cttggtaact
    20461 gaaatggtcg gcgagttccc cgaactgcaa ggcacgatgg gcaaatacta cgcccgcttg
    20521 gacggcgaaa ccgaagaaat cgccgaagct attgagcaac actaccaacc gcgttttgcc
    20581 ggcgacaagc tgcccgaaag caaaattgcc gccgccgtgg cacttgccga caaactggaa
    20641 accctggtcg gcatttgggg catcggtttg attcctaccg gcgacaaaga cccctacgcc
    20701 ctgcgccgcg ccgccttggg tattttgcgg atgctgatgc agtacggttt ggacgtgaac
    20761 gaactgattc agacggcatt cgacagcttc ccgcaaggtt tgctcaacga gaaaacgccg
    20821 tctgaaaccg ccgactttat gcaggcgcgc cttgccgtgt tgctgcaaaa cgattatccg
    20881 caagacatcg ttgccgccgt actcgccaaa cagccgcgcc gtttggacga tttgaccgcc
    20941 aaactgcagg ccgtcgccgt attcaaacaa ctgcccgaag ccgccgcgct cgccgccgcc
    21001 aacaaacgcg tgcaaaacct gctgaaaaaa gccgatgccg cgttgggcgc agttaacgaa
    21061 agcttgctgc aacaggacga agaaaaagcc ctgtacgctg ccgcgcaagg tttgcagccg
    21121 aaaatcgccg ccgccgtcgc cgaaggcaat ttccgaaccg ccttgtccga actggcttcc
    21181 gtcaagccgc aggttgatgc cttcttcgac ggcgtgatgg tgatggcgga agatgccgcc
    21241 gtaaaacaaa accgcctgaa cctgctgaac cgcttggcag agcagatgaa cgcggtggcc
    21301 gacatcgcgc ttttgggcga gtaaccgttg tacagtccaa atgccgtctg aagccttcag
    21361 gcggcatcaa attatcggga gagtaaattg cagcctttag tcagcgtatt gatttgcgcc
    21421 tacaacgtag aaaaatattt tgcccaatca ttagccgccg tcgtgaatca gacttggcgc
    21481 aacttggata ttttgattgt cgatgacggc tcgacagacg gcacacttgc cattgccaag
    21541 gattttcaaa agcgggacag ccgtatcaaa atccttgcac aagctcaaaa ttccggcctg
    21601 att
//
LOCUS       DAWTHD010000019        21437 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWTHD010000019 DAWTHD010000000
VERSION     DAWTHD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21437
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.019"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            425..792
                     /locus_tag="WM704_000606"
                     /pseudo
     CDS             425..792
                     /locus_tag="WM704_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            789..1100
                     /locus_tag="WM704_000607"
     CDS             789..1100
                     /locus_tag="WM704_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFA9340089.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            1210..1423
                     /locus_tag="WM704_000608"
                     /pseudo
     CDS             1210..1423
                     /locus_tag="WM704_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1873..2428)
                     /locus_tag="WM704_000609"
                     /pseudo
     CDS             complement(1873..2428)
                     /locus_tag="WM704_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2425..3126)
                     /locus_tag="WM704_000610"
                     /pseudo
     CDS             complement(2425..3126)
                     /locus_tag="WM704_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950970.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3363..4154)
                     /locus_tag="WM704_000611"
     CDS             complement(3363..4154)
                     /locus_tag="WM704_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFA9340090.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(4383..7013)
                     /locus_tag="WM704_000612"
     CDS             complement(4383..7013)
                     /locus_tag="WM704_000612"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFA9340091.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVSDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            7280..7796
                     /locus_tag="WM704_000613"
                     /pseudo
     CDS             7280..7796
                     /locus_tag="WM704_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            7803..8246
                     /locus_tag="WM704_000614"
     CDS             7803..8246
                     /locus_tag="WM704_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFA9340092.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            8415..10472
                     /gene="ppk1"
                     /locus_tag="WM704_000615"
     CDS             8415..10472
                     /gene="ppk1"
                     /locus_tag="WM704_000615"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFA9340093.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(10621..11724)
                     /locus_tag="WM704_000616"
     CDS             complement(10621..11724)
                     /locus_tag="WM704_000616"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFA9340094.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(11804..13360)
                     /gene="dnaA"
                     /locus_tag="WM704_000617"
     CDS             complement(11804..13360)
                     /gene="dnaA"
                     /locus_tag="WM704_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFA9340095.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            13652..13786
                     /gene="rpmH"
                     /locus_tag="WM704_000618"
     CDS             13652..13786
                     /gene="rpmH"
                     /locus_tag="WM704_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFA9340096.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            13789..14154
                     /locus_tag="WM704_000619"
     CDS             13789..14154
                     /locus_tag="WM704_000619"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFA9340097.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            14219..14440
                     /gene="yidD"
                     /locus_tag="WM704_000620"
     CDS             14219..14440
                     /gene="yidD"
                     /locus_tag="WM704_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFA9340098.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPAP"
     gene            14613..16250
                     /gene="yidC"
                     /locus_tag="WM704_000621"
     CDS             14613..16250
                     /gene="yidC"
                     /locus_tag="WM704_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFA9340099.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(16331..17041)
                     /locus_tag="WM704_000622"
     CDS             complement(16331..17041)
                     /locus_tag="WM704_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9340100.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(17103..17693)
                     /gene="maf"
                     /locus_tag="WM704_000623"
     CDS             complement(17103..17693)
                     /gene="maf"
                     /locus_tag="WM704_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HFA9340101.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            17841..18341
                     /locus_tag="WM704_000624"
     CDS             17841..18341
                     /locus_tag="WM704_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFA9340102.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            18375..18554
                     /gene="rpmF"
                     /locus_tag="WM704_000625"
     CDS             18375..18554
                     /gene="rpmF"
                     /locus_tag="WM704_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFA9340103.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            18729..19256
                     /locus_tag="WM704_000626"
     CDS             18729..19256
                     /locus_tag="WM704_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFA9340104.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDSEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            19346..20392
                     /gene="plsX"
                     /locus_tag="WM704_000627"
     CDS             19346..20392
                     /gene="plsX"
                     /locus_tag="WM704_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFA9340105.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            20530..20781
                     /locus_tag="WM704_000628"
     CDS             20530..20781
                     /locus_tag="WM704_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFA9340106.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            20808..21026
                     /locus_tag="WM704_000629"
                     /pseudo
     CDS             20808..21026
                     /locus_tag="WM704_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21186..21368
                     /locus_tag="WM704_000630"
                     /pseudo
     CDS             21186..21368
                     /locus_tag="WM704_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5326 a         6014 c         5251 g         4846 t
ORIGIN      
        1 ggcgaggcaa cgccgtaccg gtttaaattt aattcactat aaaaataaga aaaagataaa
       61 aaattggtaa caaatgcggt aacaaatggt aacgaatcgg taacaacttt tggggttttc
      121 cggtttttta ccgtcttggc agtgggagcg tagcggaatg aaaagccaaa acgcacggaa
      181 ccgcgcctat tttgagcagg aatggcggtt aaaccggttg gttatattgc ggggcatagg
      241 aagacagcga aacgcgctaa aaacgcaatt tgagccatta aaagcgattg attaaaaaat
      301 aatcaaatta gccgccggtg tttcaggcgg cataaacgga gaaattgcag ggtataaaaa
      361 ggcggcttgc cgtgttgcct gtctttggta taatccccag tatcactaag taaacggttg
      421 cacaatgcgg atattcaaaa accaatggat agtgaaattt gccaagaagc acaaaatcag
      481 tgattcagag ctgatagagg cggtagagcg ggcagataac gggctgatag atgcagattt
      541 gggcggcgcg tgattaagca gcgcatagca aggcaagggc aaggcagaag cggcggttat
      601 cgcagtctga tatttttcag gcatggagaa cgggcgtttt tcatgaccgc ctttgccaag
      661 aacgaccgcg aaaacatcac tgataaagaa cttacagaat taaagaaaac cgccgccatc
      721 atcttggcaa tgacagaaac cgacatagag caagccaagt caaacggcac tttcacggag
      781 atacagccat gaaatataaa agcgggattt tcgcagcaat acacgaaacc atgcagggat
      841 tgcacgacat cggcgcaatc gacaaaaaga ctatgcgcgg ctttgacaag tcctgcctga
      901 ccgaaatcaa gccgttgagc ggcggggaca tcaaggcaat cagggagaag gaggcactat
      961 cgcaagccgc tttcgccatc tatctcaacg tggggaaaaa tcacgtttcg gcttgggagc
     1021 ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct gaccatcgtc aaaaacaagg
     1081 gcatcgaagc cattgcgtag ccgacttggc aaacggcaaa atcagcaagt tcacaataga
     1141 cgcgctgctg aatatgcctg ccaaaacagg caagaccgcc gaactgaata tcagggcgta
     1201 gccgcataaa tgcccgaccg catcaaacca agccgaaacg gcggcggtgc agacggcata
     1261 gcccgacagc aaggcacggc gcagacgggc gcgaaacccg aaacatcccc gaccgcgagg
     1321 cacggggatt ttttgcgccc gttgcagggg ggggattgga tttaagcggc gcgggcttga
     1381 aggcaaaacg ggcggggcgc agaactgttt aaatgccgtc tgaatctcaa acgatttcag
     1441 acggcatttt gaaacaacgg ctcaaattct cgatcccctt cccttaacgc cgacgttttt
     1501 tattaacgcg ccccttattt ctgacacttt gctcataaac cggcataacg gtcggcaaca
     1561 accgttttag attttctata caggcattgt ttacagcatc agcataagac accgtactct
     1621 ggaataccgc ctaaaccctg cggatagttt tggatgtggt atcgacattg tcggcagact
     1681 tgtttaactc gaccgttttc aaataatctt tgacagatgc tgcattcagt gtcgcggaat
     1741 ccgcgtattt tgaacaaaag gtcgggctac tttatcacaa tagcagaaag atggaaaacg
     1801 gcggggattg gtgaaaggtc gtctgaaatg atattcagac gaccttgaac ggttgtgttt
     1861 taaatatggt atttattcat ttgtttcacc atcaggcagt ccaaaccacc ttaagggaat
     1921 cgtgttaaat tggtaatggc agttaacact gataatataa tctaaaacat ctttaatatt
     1981 ccggttttgt aaaccaatca ttcaaaacat agacatatcc aacccgccaa cctaaagagt
     2041 gaactaactt tgtatattgt tttctcttaa aatcgcaagt ttgtaatttc tcatctactg
     2101 aaccgggagt ttgttggaat ttgatctcaa taataaataa ggtatcacgg atgatgataa
     2161 ataagccatt atcgggcagt aatcttttag acagatggtc tttccaatta atattgtatt
     2221 ccagtctttc caaaaatcgg taaaactcat attttttgaa acaataagcc ttcaattctc
     2281 cattaaacca aacttcataa cccgcattat catctgtttt tcttaagtcg tacccgggaa
     2341 tttcttcaaa caactttcgt aagtctgtac gttcttcgaa tctgagtcct gtttgtgtcc
     2401 ttgcaccgcc gattccgcca ataatcattt ttctaacctt ttctttgcta attcaaaaaa
     2461 atcttcttcc agttcgcaac cgcaaaatct ccgaccatgt tttaaggcgg caacgcctgt
     2521 tgtgccgctg cccataaaag ggtcaaaaat taaatctccg atatttgaag ccgatagtat
     2581 gcagcgttca agtaagggga gtggtttttg tgtcggatgt ttgccgaatg ttttttcggt
     2641 tttatttggt ggaggaaatg tccaaacaca tttcatctgt ttgccatcat tttgtgtttt
     2701 catggtttca taatgaaacg tgtgtttagc ttttttattt ttctttgcct ataagattgt
     2761 ttctgtcgaa tgggtaaaga aacggcagga caaattaggg ggaggattgg gtttttccca
     2821 agtaatattg ttcaaaatat ggtagccgac ggtttgcatc aggtagccga ttaaatagat
     2881 attatgaaat gtgccgcaaa cccaaattgt gccgtttggt tttaataagg cgtaacacag
     2941 tcggagccat tcttcgtaaa attccaaatc tgccgccatt cctttggatt tatcccagtt
     3001 gcccttattg acggaaacca tttgcccgtt ttgacaactg aaaccgtcat tggaaagaaa
     3061 gtaaggcggg tctgcgaaaa tcatatcaaa acagccgttt ggatgttttt ccaatatctt
     3121 ccgcatgacg ttaagggaat tttcgttgta gaggacaagg tcgtctgaaa cgattttttc
     3181 aaacgacttt gataaatggc agcttcttca gccggtttgt caaaatcaat cataaattgc
     3241 caataattct ccgacttttt tacgactgct gccctttgcg gcaatattgc gttgtacttc
     3301 aaccgttttt agaatggcag aagaataaat atttctcgta aattcagtat catgatttga
     3361 aatcaaaacg ccttgggagt tgggtgcaat ttgtcgattt ttttgtaaag tccgcgacca
     3421 atgaattcga tcatattttg atcacgcaaa atttgcaact gttggcggat tttgtctttg
     3481 atatggttgt tttggggaaa ttggatggat agtttgtttt caaattcata catttgcgac
     3541 aatgtgaatt cttcggtgag ttggtcgata catttcataa cagccaaaag ccagtctttg
     3601 cgctccgcat tttggttgcg taaaaacagg ttggattgcc attttttcag aacggtttcg
     3661 ggttcgataa tgcgggaatc gtctattaag aatatttttc cgctttcagg caaaggggcg
     3721 agattgatgg aacacataat gtggtgcggt cggtttttaa tgcctttatt tctgggaata
     3781 atcatatccg gcgtgatgaa atgtttgggt acaagcacca attgccgtat ggagtaatcc
     3841 gcttttttat atgcaagaaa gaaaaagttg gggttggtat ctgaccggat gcgctccaac
     3901 atagtgcgat atgcaccgtc aggcacgctg ttgcctatgg ttttttgatt tttactcttt
     3961 aattcatatt gctcgtggca atttgggcaa aagaggtctg caacaggttt gttattggca
     4021 aatctctgca tcggcttgct tccgcaacag gggcaatagc cgtttttttc cagccaagcc
     4081 tcgctcatta cacggatttt gtgggttgct ttattttgtt gctttcccaa ttgggtatcg
     4141 aaaaataaat tcatattttg gattttgaga ttaattttta gtagattaac aaaaatcagg
     4201 acaaggagac gaagccgcag acagcagggc aaggcggggc aacgccgtac cggtttaaag
     4261 ttaatccgct atatttgagg tctgtcttgc agacaaccca atctgcctta aaaaggccgt
     4321 ctgaaaccct gtttccaagt ttcagacggc ctttatccgt atggctaaac cttaaaagcg
     4381 gtttagacga cgatgttcac cagtctgccc ggtacgacga tgattttctt cgcaggcttg
     4441 ccttccatga atttcactgc gccttcgttg gcgagtgcgg cggcttcgag gtcggctttg
     4501 gaggcgtcgg cggcgacggt gattttgccg cgcagtttgc cgttgacttg aaccatcact
     4561 tcgatttcgg atttgaccag ggcggcttcg tcgactgtcg gccagcctgc ttcccacagt
     4621 ttcgcgccgt tcaattcgct ccacagggtt tcgcagatgt gcggcacgat gggccacaac
     4681 aggcgtacgg cggcttccaa tacttcttgg gcgacggcgc ggccttgttc gctgccggtg
     4741 tcggttttgt cgtattggtt gagcagttcc atcacggcgg cgatggcggt gttgaactgc
     4801 tggcggcggc cgtagtcgtc gctgactttg gcggtggtgg aatgcagttt gtgacgcagg
     4861 tctttgagtt ctttagacaa accgtcttgg ttgcctgcaa atgctttgac cgcgccgcct
     4921 tgcttcaggt attcgtaaac ggtacgccac agacggcgca ggaagcggtg tgcaccttcg
     4981 acgccgctgt cgctccattc gagggactgt tcgggcggtg cggcgaacat catgaacagg
     5041 cgggcggtgt ccgcgccgta ggcgttaatc agttcttgcg gatcgacgcc gttgtttttg
     5101 gatttggaca ttttttccgt gccgctgatg acgacgggca gtccgtcggc tttgaggacg
     5161 gcggaaacgg ggcggccttt gtcatcgaaa gtcagctcga catcggcggg gttgatccag
     5221 tctttgccgc ctttgtcgtt ttcgcggtag taggtttcgc agacgaccat accttgcgtg
     5281 agcaggcgtt caaacggttc gtcaacattg accaaacctt cgtcgcgcat cagtttggtg
     5341 aagaagcgcg cgtacaggag gtgcaaaatc gcgtgttcga tgccgccgat gtattggtcg
     5401 accgcgcccc agtatttcgc ggattctgcc gataccatgc cgtctgaaaa cttgggcgac
     5461 atatagcgga agaagtacca gctcgactcc atgaaggtgt ccatggtgtc ggtttcgcgt
     5521 ttcgccgcgc cgccgcagca cgggcaggaa gtttcgtaaa actcgggcat ttttgccagc
     5581 ggcgaaccca taccgtcggg tacgacgttt tcaggcagga cgacgggcag ttggtcggca
     5641 gggacgggaa cgtctccgca tttttcgcaa tggacgatgg gaatcgggca gccccagtag
     5701 cgttggcgcg aaatgcccca gtcgcgcagg cggtattggg ttttcggttc gcccgcacct
     5761 tggctttgca gcttggcggc aacggcatca aatgccgtct gaaaatccaa gccgtccaag
     5821 tcgccgctgt tgaccaatac gccgttttct ttgtcgccgt accattcttg ccatcggttt
     5881 gcgtcgaatg cgttgtcgct gacggcaatg acttgttttt tcggcagatt gtatttggcg
     5941 gcaaactcga aatcgcgttc gtcgtgcgcc ggaacagcca tcaccgcgcc gtcgccgtag
     6001 ccccacaaga catagttggc aatccacact tccagcttgt cgccgttgag cgggttgacg
     6061 acgtagcggc cggtcggcac gccttttttc tccatcgtcg ccatatcggc ttcggcaacc
     6121 gaaccggctt tgcattcggc gataaatgcc tgcaattcgg gtttgtcggc ggctgcggct
     6181 gttgccagcg gatgttcggc ggcaacagcg acataagtcg cacccatcag cgtgtcgggg
     6241 cgggtggtat aaacttgcag gaatttcgcg taatcgcctt ccaaaccttg cttgctgtcg
     6301 tctgaaacgg cgaagcgcac ggtcatgccg cgagatttgc cgatccagtt gcgctgcatg
     6361 gttttgactt gttccggcca gtgttccagc ttgtccaaat cgttgagcag ctcttcggcg
     6421 taatccgtga ttttgaagta atacatcggg atttcgcgtt tttcgatcaa cgcgcccgaa
     6481 cgccagccgc gtccgtcgat gacttgctcg ttggcaagga cggtttggtc gaccgggtcc
     6541 cagttcaccg tgccgttttt gcgatagacg atgccttttt caaacagctt ggtaaacagc
     6601 cattgttccc agcggtagta ttcgggtttg caggtggcga cttcgcgttc ccagtcaacc
     6661 gcaaaaccca ggcttttgag ctgggttttc atgtattcga tgttgtcgta ggtccaagcg
     6721 gcgggggcga cgttgttttt catcgccgca ttttccgccg gcatgccgaa cgcgtcccaa
     6781 cccataggct gcataacgtt gaagccgttt aaaagtttga agcggctcaa tacgtcgccg
     6841 atggtgtagt tgcgtacatg ccccatatgc agcttgccgc tagggtaagg gaacatcgaa
     6901 aggcaataat atttgggttt ggaagcgtct tcggagacgt tggaaatacg ggcgtcgtcc
     6961 cattttttct gcgccgcagg ctcgatggcg gcgggctggt aatgttcttg catggtcatt
     7021 ttgtttttcg cttgaaaacg ttaggaattt aaaggaggaa ttataacagg ttgccgatgt
     7081 cggcgaatcg gcaaattgcc ggcaggatgc gtaaattcgc acgcgcatca ttccgtatgc
     7141 cgtacaaata caccgcgttt actgatacgc acgtttttta tgctaatatt acgaaccaaa
     7201 atcaaacgtt taaaactctc ctgatgcggc tcttccgaac aaaaggcaga cgggcatcgg
     7261 gtaaaaaagg attctgcata tgaaaatcaa acaaatcgtc aaaccgagct tggcagtatt
     7321 ggcggcaggc attctgtctg cctgcgcaac cataaagcaa cgtcaaagcc gacggcacga
     7381 ccgacaatcc ggttttcccg gaaccctatt ccgtaacgct cgacaacaag cgcggcacat
     7441 tcccgactta tgacgaactg gatcagatgc gccccggtct gaccaaagac gacatctaca
     7501 aaatcccggg ccgcccgcat tacgacgaag gtatgtacgg cgtgcgcgaa tgggattacc
     7561 tgttccactt ccacaccccg ggcgtgggta tcgaccctga aaacacttcc ggcgtggaag
     7621 gcgttactac ctgccaatac aaagtgattt tcgataaaga caaatttgcc cgcagcttct
     7681 actggaaccc cgtcttcccg aaagatgccg tctgcccgcc gcccgcaccc aaagccgagc
     7741 cgcaagtcat catccgcgaa atcgtgccgg caaaaccgaa acgtatccgc caataagccg
     7801 ctatgccgcc ccgcctgctt tcagggatat tgtgctgcct gctgacgact gcccccgtat
     7861 tcgcacaggg gcagccggat gcggtcagtg cctatattca aaagaaaaaa gtcatcgtcg
     7921 acacatccaa ggcggaactc tgttttgccg acgaccgtca gtgccacccc gtcctcatcg
     7981 gcactgccac acccaagggg acgttcggac tgacgctgaa cagtaccgac aagcccggtt
     8041 acggcggcga agtcatcggt ttcaagcagg agggcgattt tcttttcgcc ctgcaccgcg
     8101 tttggaatca gataccgtcc gaaaggcgaa acgaacgcat cgcctccccg tccgtgtccg
     8161 acaggattat gaccaacggc tgcatcaacg tcagcgatgc ggtgtacgaa aaactgcgtc
     8221 attattttgt gttggaagtg atttgaaaca gacggatacc gcacgtaccg gtatccgttt
     8281 tcacattgcc ccgatgcctg aaacagaccg tccgtcacgt catgccgcct gaaaccggcg
     8341 cagatgccgc caagccttca gacggcattg tccgcccgct ccgaccgaac aacaaccatc
     8401 tttgggagaa ccttatgacc gaacaaaacc gcatcctctg ccgcgaactg agcctgctgg
     8461 cattcaaccg ccgcgtgttg gcacaggcgg aagacaaaaa cgtccccctt ttggaacgcc
     8521 tgcgcttcct gtgcatcgtg tcgtccaatc ttgacgagtt ttttgaagtc cgcatggcgt
     8581 ggctcaagcg tgaaaacaag ctgcaccccc ggcgcaggcc ggacaacggc aaaatgccgt
     8641 ctgaaaccat cgccgacgtt accgaagcgg cgcgctccct gatacggcac cagtacgacc
     8701 tgttcaacaa cgtccttcag cccgagttgg cgcgggaaag catccatttt taccgccgcc
     8761 gcaattggac gggcacgcag aaaaaatgga tcgaagacta tttcgaccgc gaactgctgc
     8821 cgatcctgac ccccatcgga ctcgaccctt cccacccttt cccgcgcccg ctgaacaaat
     8881 cgctcaactt cgccgtagaa ctcgacggca cggacgcgtt cggcaggcct tcgggtatgg
     8941 cgattgtcca agcaccgcgc atcctgccgc gcgttgttcc cctgccgtcc gaactgtgtg
     9001 gcggcggaca cggcttcgtc ttcctgtcgt ccatcctcca cgcccacgtc ggcaaactct
     9061 tcccgggcat gaacgtcaaa ggctgccacc agttccgcct cacgcgcgac agcgacttga
     9121 ccgttgacga agaagacgta caaaacctcc gcgccgccat tcaaaacgaa ctgcacgacc
     9181 gcgaatacgg cgacggcgtg cggctcgaag tcgccgacac ctgtcccgcc tacatccgcg
     9241 actttctgct cgcgcaattc aggctgaccg acgccgaact ctatcaggtc aaaggcccgg
     9301 tcaacctcgt ccgcctcaac gccgtccccg acctagtcaa ccgccccgat ttgaaatttc
     9361 cgccgcacac gccgggccgt ctgaaagcct tgggcaaaaa cagcccgatc ttcgatttgg
     9421 tgcgccaatc gcccatcctg ctgcaccacc cctaccaatc tttcgatccc gtggtcgata
     9481 tgatacgcga agccgccgcc gatcccgccg ttcttgccgt caaaatgacg atttaccgca
     9541 ccggcacgcg ctccgaactc gtccccgccc tgatgaaggc ggcactcgcc ggcaaacaag
     9601 taaccgtcgt cgtcgaactg atggcgcgtt ttgacgaagc caacaacgtc aactgggcga
     9661 agcagctcga agaggcggga gcgcacgtcg tgtacggcgt gttcggctac aaagtccacg
     9721 ccaaaatggc actggtcatc cgccgcgaag acggcgtgct caagcgttac gcccacctcg
     9781 gcacgggcaa ctaccaccaa ggcacatcgc gcatctacac cgacttcggc ctcattaccg
     9841 ccgacgaaca aatcaccgcc gacgtgaaca cattatttat ggaaatcaca ggcttgggca
     9901 aacccgggcg gctgaacaaa ctctaccaaa gtccgtttac cctgcacaaa atggttatcg
     9961 gccgcatcgc tcgcgaaacc gaacacgcaa aagccggcaa accggcgcgg attaccgcca
    10021 agatgaattc gctcatcgaa ccgactgtca tagaagccct gtatcgggca agcgcggcag
    10081 gcgtgcaaat cgatttgatt gtgcgcggta tgtgtacctt gcgcccgggt gtaaaaggct
    10141 tgtccgaaaa catccgcgtc cgctccatcg tcggcaggca gctcgaacac gcgcgcgtat
    10201 attgtttcca caacaacggc gcggacgaca cctttatttc cagcgcggat tggatggggc
    10261 gcaatttctt ccgccgcatc gaaaccgcca ccccgattac cgcgcccgaa ctcaaaaaac
    10321 gcgtcatccg cgaaggtttg gaaatggcac tggcagacaa tacccacgcg tggctgatgc
    10381 agcccgacgg cggctatatc cgcgccgcgc ccgccgaagg cgaaagcgaa gccgacctgc
    10441 aaaacgattt atgggatttg ctccgaggct gacccgcaca agcacttccc ccatacgctc
    10501 ccaaccccaa tccgccgcat tccagacggc attgcctttc cgacagccga acaaaacccc
    10561 gaatccgtct gcacggttcg gggcatatct ccaatacggg catcgtgttc cggaaaaccg
    10621 tcaaatccgc atcggcatca caatatattt gaaattcgga ttgttcggca cggtaaacag
    10681 cgtcgagcgg ttggcgtcgc cgaaggcaag ctgcatatcg tcggaatgga tgttgcgcaa
    10741 cacgtccatc aaatagccga tattgaaacc gacttcgagt tcgccgccct gataggcgat
    10801 ttcgatttct tcgcgcgctt cttcctgctc gttgttgctg cacacgacgc tcaacaggcc
    10861 gggttgcagg aacaggcgcg cgccacggaa tttttcgttg gcaagaatgg acacacgttc
    10921 cagcgcgccc aaaagttcgg cacgggacaa cacgaaaatc ttgtcgttgt ccaaaggaat
    10981 cacgcggttg aaatcgggga atttgccgtc gatgaccttg ctgacgatgg tcgtgccgtt
    11041 gcattggaaa cgcacctgct tgtccagcag ctcgatttga atcggatcgt cggggttgtt
    11101 caacagtttg aacagttcca gcaccgtttt gcgcggcaaa atcacttcgg cgcgcggcaa
    11161 atccgcatca atcgcgcagg ctgcataggc aaggcggtgt ccgtcggtcg ccacaaggcg
    11221 caactggctg ccctcaacct gcatcagcag accgttgaga taatagcgga tgtcctgcac
    11281 cgccatgctg tactgcactt gcgacagcat ggttttgaaa cgctcctgcc ccagcgagaa
    11341 agtcgcgctg atgtcctcgc cgacattcat catcggaaaa tcggcggcag gcagggtttg
    11401 cagggcaaaa cgcgatttgc ccgccttcag cgtcagacgg ttgtcgtccc aatccagcga
    11461 caccagcgca ccggcaggca gcgcgcgcaa aatgtcctga aatttcttgg cattggtagt
    11521 gatgcggaag tcgcccgcac ctccctcggg acccgcggtg tcgatttgga tttccaaatc
    11581 ggttgccaag agtttggtct gaccgcccct gccctcaatc agcacattgg acaggatggg
    11641 cagggtgtgt cggcgttcga cgatgccggt aacggcttgc agcggcttga gcaggctgtc
    11701 gcgctcggct tgtaaaatca acattttcgc tccttaaatc ggttggattt cctacccttc
    11761 ggacggcatg gtcaatgtca tgccgtctga aaggcgtgtc cggtcagttt tgaatcagaa
    11821 tcagcagttt ctcgtaatcc tgcgccaact cggggtcttc ctcgcgcagt ttcgccaccg
    11881 ccctgatgcc gtgcatgacg gtcgtatggt cgcgtccgcc gaacgaatcg ccgatagacg
    11941 gcaggctcaa agtggtcaat tctttggtca ggctcatggc aacctgacgc ggacgggcaa
    12001 tgttgcgcgt gcgttttttg ccgagtacgt cgctgatttt gatgcggtaa tatttcgcca
    12061 ccgcatcgat gatgatgtcg gcggtgatga ctttgtgctt ctcggcaata atgtcctgca
    12121 aagcggtacg cgccaaatcg atgtcgatga cgggacggtt cataaagcgg ctgctcgctc
    12181 cgacacggtt gaacgcgcct tccagttcgc gcacgttgga acggatcaga ttggcaatga
    12241 acagcgcggc ttcgtcttcg atactgatgc ccgccgcttc cgcctttttc tgcaaaatgg
    12301 cgatacgcat ttccaattcg ggcggctcga gttccaaagt cagcccccac gaaaagcggg
    12361 atttgaggcg gtcgtccata ccttcgattt tggcgggtaa aacatcgcaa gtgaggatga
    12421 gctgtttttt ctcattgtga aaatggttgt acagatagaa aaattcttcc atcgtacggt
    12481 cttttccttt gatgaactga atatcgtcga taatcagcag gtcgtattgc ttgtattgct
    12541 gcttgaacac gtcgtaggta ttgttgcgaa ccgccttcat aaagctgcgg atgtagtcgt
    12601 ccgaatgcat atagcgcact ttggcatcgg gacggttttt caacagctcg ttgccgaccg
    12661 cctgcacaag gtgggttttg cccaaacccg tgctgccgta caggaagaac gggttgtaac
    12721 tctgccccgg gttttccgca atcgcctgcg ccgcagccgc cgcaaggcgg ttgcccttac
    12781 cttctaccaa cgtatcaaac gtgtaatccg gagacaggtt ggtttgttcg taacgcgctt
    12841 cttccgcatc acgctgcgca tccgtccgcg ctttggcaac tgccgccgat tccggccggg
    12901 aagcaggccc ggcagcctga cgcggctcgt gcggcaggtt tttcatacgt tccgccaaaa
    12961 tatccgccgc cgttttcgac gcagcgggtt tgacaggctt ttcagacggc agctcgtcca
    13021 acagaacctc ctgcacgggc atctcctccg accccgcgtg caaggacggc tcggcaggtt
    13081 cgacagcacc ttcaaccgcc gccatctcat aacgcacgcc ttctcccggt ttgaatacga
    13141 aggcgggacg gccggcagcc aactcttccc tcaccgcttc tatttttccg gcaaactggc
    13201 tcttgagcat attgcaggca aactggttct tgccgtacac cacccatacg ccaccctcct
    13261 caccaaccgt aaggggcgca atccattgcg caaactgccc gtgaggcaac atatcgtgaa
    13321 gacggcggag gcacagcggc caaaactctg ctaatgtcat ggataggctc gaatcggtaa
    13381 aaatgaaatc gaaaacaaag aaaatataat attttcaaaa agaaaacaaa tctgttgaat
    13441 acgcgtcggt tcaaaacgca gcgaaccgat tataccgact cgccgatatt ttatccacaa
    13501 cccgtgcaaa aatttatcca cagaaaggcg gcagaaatcc gcaggcaatc gggcaatcct
    13561 cctgcaaagt ttctatattg attgacaaaa gcggcaaatt ggagtgtaat tcacggttta
    13621 attatctacc cattctattt taggaaccat catgaaacgc acttatcaac cttccgttac
    13681 caaacgcaaa cgcacccacg gcttcctcgt gcgctccaaa acacgcggcg gacgcgcagt
    13741 attggccgca cgccgtgcca aaggccgcaa acgcctggca gtgtaatttt ggactaccgc
    13801 ttcggaaggc agtaccgctt gttaaaaacg gatgattttt catccgtttt tgcattcaga
    13861 aaccgccgca gccgcgacct gctgcaagtt tcgcgctcaa acggcaacgg gctggaccat
    13921 ccccgcatcg gtctggtggt cggcaaaaaa accgccaaac gcgccaacga gcggaactat
    13981 atgaaacgcg tcatccgcga ctggttcaga ttgaacaaaa accggctgcc gcctcaggat
    14041 ttcgtcgtgc gcgtccgccg gaaattcgac agggctaccg caaaacaggc aagggcggaa
    14101 ctggcacaac tcatgttcgg caaccccgca accggatgcg ggaaacaggt atgatcagaa
    14161 cggtaccctg caggcaaggt tcagacggca acgggtttcc catacaagga acatcccgat
    14221 gaacttccta ttgtccaaac tgctgctggg gctgataagg ttttaccaat actgcatcag
    14281 cccgctgatt ccgccgcgct gccgttatac gccgacctgt tcgcaatacg cggtcgaagc
    14341 ggtcaaaaaa tacggcgcat tcaaaggcct ccggctcgcc atcaagcgca ttgcacgctg
    14401 ccaccctttc ggcggacacg gacacgaccc cgctccctga cccgacgcaa tattcaaatt
    14461 gcacgctttc cttttatttc ccatcggttt ctatataatg ccgtctgaac cttcgggctg
    14521 gcggcacggc cgtcgggtat gaagcccgcc cttattcccc gtctatcgga acacgcaacc
    14581 tgcggcattt ccgaccattc aggaaactct atatggattt taaaagactc acggcgtttt
    14641 tcgccatcgc gctggtgatt atgatcggct gggaaaaaat gttccccacc ccgaaacccg
    14701 tccccgcgcc ccaacaggcg gcacaaaaac aggcagcaac cgcttccgcc gaagccgcgc
    14761 tcgcgcccgc aacgccgatt accgtaacga ccgacacggt tcaagccgtt attgatgaaa
    14821 aaagtggcga cctgcgccgg ctgaccctgc tcaaatacaa agcaaccggc gacgaaaaca
    14881 aaccgttcgt cctgtttggc gacggcaaag aatacaccta cgtcgcccaa tccgaacttt
    14941 tggacgcgca gggcaacaac attctgaaag gcatcggctt tagcgcaccg aaaaaacagt
    15001 acaccctcaa cggcgacaca gtcgaagtcc gcctgagcgc gcccgaaacc aacggactga
    15061 aaatcgacaa agtctatacc tttaccaaag acagctatct ggtcaacgtc cgcttcgaca
    15121 tcgccaacgg cagcggtcaa accgccaacc tgagcgcgga ctaccgcatc gtccgcgacc
    15181 acagcgaacc cgagggtcaa ggctacttta cccactctta cgtcggccct gttgtttata
    15241 cccctgaagg caacttccaa aaagtcagct tctccgactt ggacgacgat gcgaaatccg
    15301 gcaaatccga ggccgaatac atccgcaaaa ccccgaccgg ttggctcggc atgattgaac
    15361 accacttcat gtccacctgg atcctccaac ctaaaggcgg ccaaagcgtt tgcgccgcag
    15421 gcgactgcca catcgacatc aaacgccgca gcgacaagct gtacagcgca agcgtcagcg
    15481 tgcctttagc cgctatccaa gccggcgcca aagccgaaac tgccgtcaac ctgtatgccg
    15541 gtccgcaaac cacatccgtt atcgcaaaca tcgccgacaa cctgcaactg gcaaaagact
    15601 acggtaaagt acactggttc gcatcgccgc tcttctggct cctgaaccaa ctgcacaaca
    15661 ttatcggcaa ctggggctgg gcaatcgtcg ttttgaccat catcgtcaaa gccgtactgt
    15721 atccattgac caacgcctcc taccgttcga tggcgaaaat gcgtgccgcc gcacccaaac
    15781 tgcagaccat caaagaaaaa tacggcgacg accgtatggc gcaacagcaa gcgatgatgc
    15841 agctttacaa agacgagaaa atcaacccgc tgggcggctg tctgcctatg ctgttgcaaa
    15901 tccccgtctt catcggcttg tactgggcat tgttcgcctc cgtagaattg cgccaggcac
    15961 cttggctggg ctggattacc gacctcagcc gcgccgaccc ctactacatc ctgcccatca
    16021 ttatggcggc aacgatgttc gcccaaacct atctgaaccc gccgccgacc gacccgatgc
    16081 aggcgaaaat gatgaaaatc atgccgttgg ttttctccgt catgttcttc ttcttccctg
    16141 ccggtttggt tctctactgg gtggtcaaca acctcctgac catcgcccag cagtggcaca
    16201 tcaaccgcag catcgaaaaa caacgcgccc aaggcgaagt cgtttcctaa atgccgcagc
    16261 atgaaaaatg ccgtctgaaa cctgttcaga cggcattttt attgttcacc ccctatcggg
    16321 gcggaaatct tcaacccgca tacatcacaa aaatcgtcgg gcgttttttc agattgggca
    16381 tttccttcat cctgcgccaa tccgcaaccg ttttactgac caccaactga gtcggcaacg
    16441 tcaaatccgt agccgtacac aagcgcgttt caggatgcag gttttccacc gcatcggcaa
    16501 gcagcgcatc attgcggtaa ggcgtttcaa taaaaatctg cgtctcgccg cactgccgcg
    16561 aacgttgctc caaagcattc aaactctgaa tgcgctcatt tttttcagac ggcagataac
    16621 cattaaacgc aaagttctgc ccattcgcac ccgaagccat cagcgccagc agcaggctgg
    16681 acgggccgac caaaggccgt acttcaaaac cgtgtttatg cgccaatgcc accaaattcg
    16741 cgcccggatc ggcaacagcc gggcaacccg cctcgctgac aatccccata ctgcgccctt
    16801 cttgcaaagg tttcagcaat tccggcaaag tcttcaaatc cgtgtgttca ttcaacgttt
    16861 gcagattcag ttcgcgtatc ggcgtcgtta tccccaaatg cttcaaatgc gcacgcgccg
    16921 ttttttccgc ttccacgaca aaatccgtca gcccgacaac cgcccgttgt tcatgctgca
    16981 acaggcacgg cgtgtcaggc gtacccaaag gcgtaggaat caaatacaaa acaggagaca
    17041 tcattccctc actcatcggt taaaaatgcc gtccgaaccc ttcagacggc ataaacgggc
    17101 agttacaaaa catccacgcc ctcgtttttc aaaaaatcga ccagacggaa aaccggcaga
    17161 ccaatcaagg cattcggatc ggtactttca atccgttcga tcaacagcgc gcccaagccc
    17221 tcgcttttcg ccgcacacga acaataaacc gcatccggct cgcgctccaa atagcggagg
    17281 atgtgcagct cgtccaactg cctcatcacg accaccgtct tatcgatatg ccgatgcatc
    17341 ctgcccgtaa ccgtatttag cagaacgacc gcactgtaaa actcaatctc cctgccgctc
    17401 aaatgcatca acatcttttg cgcgttggca aggttcatcg gcttgcccca ctgcctgccg
    17461 tcgcaccacg ccacctggtc cgcaccgaca atcaacgccc cggggaaacg cccggccaac
    17521 gaccgcgcct taccctcggc aaggcgcaat gccgtctgag gggcggattc ccccaacatc
    17581 ggcgtttcgt caaaatcggg ggatgccgcc tgaaaggcaa tgccgagcct ttccatctgt
    17641 tcgcggcgga aaaccgaact cgtacccaaa accaaaggca gttccaaacc catcccatcc
    17701 tccttaccgt tgaaaacacg cccgaagggg cggtaaaatc cagccatgcg ccgaaacacg
    17761 gacgcccgcc ttcggcgtac cgcaacattt ttcttaaaaa tattgacgtt agaacatcta
    17821 aattatatca taccccgttt atgtcagacc ctaatttgat tgacccggaa atttttgccg
    17881 ccgaaaggca gaacctgcaa ggcagttttc tgctggaaga attggacgag cgagtcagtt
    17941 tgcacgatta tcccgccgac aggcggacca aaatatcgtt tacactgacc ggcggtcgcg
    18001 accggctgca acgcctgttc ctcgacctga acgtcaaagc cgatatgccc ctgatttgcc
    18061 agagatgtat caaacccatg ccgttcatgc tcgatgaaag cagccgtatc gtcctgtttt
    18121 ccgacgaaga gtccttggac gaatccatgc ttgccgacga agaactcgaa ggcatactga
    18181 ttgaaaaaga actcgacgtg cgcgcattgg tagaagacca aatcctgatg tccctgccct
    18241 tttcgccgcg acacggacac tgcggcaata cccttccgga atccgccaac caagacaaac
    18301 ccaacccctt tgctgttttg gcgggtttga aaagcagtta attaggacac agtttattta
    18361 tctaggagct tgaaatggcc gttcaacaaa acaaaaaatc cccttccaaa cgcggtatgc
    18421 accgttcgca cgacgcactg accgcgcccg cactgtttgt cgacagcaca accggcgaag
    18481 tacaccgccc gcaccacatc tcccccaacg gtatgtaccg cggccgcaaa gtggtcaaag
    18541 ccaaaggcga ataatcccta ttcgactgac tgaaaaagcc agaacattgc catgcaatta
    18601 ctggcttttt ttacattgga cgcaccatcc gtccaaactt tcgccatacg tcaacacaca
    18661 ggggcaaagc gttccgtata ataccccgtg aaaatattcc aaaagcccca accaccaagg
    18721 aaattccgat gaaacagaaa atctggtaca cctacgatga catccaccgc gtcatcaaag
    18781 cattggcaga aaaaatccgg aacgccggcg tcaaatacga tgccatgatc gctatcggcg
    18841 gcggcggctt tattccggca cgcatgctgc gctgttttct ggaaattccg atttatgccg
    18901 taaccaccgc ctattacgac agcgacagcg aaggacaagt taccgaagaa gtcaaaaaag
    18961 tccaatggct agaccccgtc cccgaagtcc tgcggggcaa aaacgtgctc gtcgtcgatg
    19021 aagtggacga cagccgcgta accatggagt tctgcctgaa agaactgctc aaggaagact
    19081 tcgacactgt cggcgtcgcc gtcctgcacg aaaaaatcaa agccaaagca ggcaaaatcc
    19141 ccgaaggcat tccctatttc agcggcatta ccgtagaaga ctggtggatc aactatccgt
    19201 gggacgcact cgacatcgac gaacacaacc gccttgccga agccggccga ggctaaccct
    19261 ttcagacggc atattttccg aaccgatgcc gtctgaagcc cgcacgaccc ctgccgcaga
    19321 ccgaaaatct accggagaaa cccctatgat tacattggcc gtagatgcca tgggcggcga
    19381 ccaaggactt gccgttaccg tacccggcgc aaccgcattc ctccaagcac accccgatgt
    19441 ccgcctgatt atgaccggcg acgaaacgca gctccgccaa gccctgaacg cggcaggcgc
    19501 gccgatggaa cgcatcgaca tctgccatac cacccaggtt gtcggcatgg atgaatcccc
    19561 tcagtccgcc ctaaagaaca aaaaatactc ctccatgcgc gttgccatca accaagttaa
    19621 agaaggcaaa gcccaagccg ccgtatccgc aggcaacacg ggcgcgctca tggcaaccgc
    19681 acgtttcgtc ctcaagacca tacccggcat cgaacgcccc gccatcgcca aattccttcc
    19741 ttccgacacc gaccacgtta ccctcgccct cgacctcggc gcaaacgtcg actgcacacc
    19801 cgaacagctt gcccaatttg ccgtcatcgg cagcgaactc gtccacgcgc tccatcctca
    19861 aaaaggacag ccgcgcgtcg ggctggtcaa cgtcggcaca gaagacatca aaggtacgga
    19921 taccgtcaaa caaacctaca aactgctgca aaacagcaaa ctcaacttta tcggcaacat
    19981 cgaaagcaac ggcatcctgt acggcgaagc agatgtcgtc gtcgccgacg gctttgtcgg
    20041 caacgtcatg ctcaaaacca tcgaaggcgc agtcaaattc atgagcggag ccatccgccg
    20101 cgaattccag agcaacctgt tcaacaaact tgccgccgtt gccgccctac ccgccctcaa
    20161 aggcttgaaa aacaaactcg atccgcgcaa attcaacggg gccatcctgc tcgggctgcg
    20221 cggcatcgtc atcaaaagcc acggcggcac agacaaaacc ggtttccgct acgcactcga
    20281 agaagcctac cacgaagcca agtccgccgg cctttccaaa atcgaacaag gcgttgccga
    20341 acaactcgcc gcactggaag ccgcccaaaa cgaaaccgcc gccagtctgt aacacgcacg
    20401 atgccgtctg aacgcccccg cccctttcag acggcatccg gcacccgtcc aaacctgcgg
    20461 gcgcggacgg cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggagt
    20521 atttgaaaaa tgaagacatt agaaaaacgg atgaaagctc tagacaaacg gatgatgaag
    20581 ttcggaaaat cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat
    20641 attcattatt cggaacgttt tttggctttt gacatcctgt gtacttatat cgaagatttc
    20701 gatatccggc tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag
    20761 atagaaagca cgtccgatta accaataaag ccaatgattt gataagaatg aaaacatcga
    20821 cggtcgtttt tggcggattt tttatggcag acaacgggga gcgaatccaa atccccgttt
    20881 tggaaaatcc tgacattagg gaaatcaatc gctttttttc cgtatcaaat tttgagaaaa
    20941 aagccggcgt ccttgttttc agaatcatcc ccgagccgga atttggcaat accgaattaa
    21001 ctgtctattt tgaaaaagga tattatagtg gattaacaaa aaccggtacg gcgttgcccc
    21061 gccttggctc aaagggaacg attccctaag gtgcccaagc accaagtgaa ccggttccgt
    21121 actatccgta ctgtccgcgg cttcgccgcc ttgtcctgat ttttgttaat ccactatatt
    21181 tgccaataat tcaaacaatt ttagaaggcg gggatattaa agtaaaaaac cttaaaacag
    21241 aaaattacag cgggaaggca agggaaatct tgggagattg ctaccctatt gaacatatct
    21301 ctaaaaatat ttctacaatt caaaatatca tttccgaatt tatcagacaa aaacaaacac
    21361 cggcaccaaa taatttgatg gaaacccaac aatcaagatg ccgtctgaag cagcttgggc
    21421 tttcagacgg catttcc
//
LOCUS       DAWTHD010000020        21234 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWTHD010000020 DAWTHD010000000
VERSION     DAWTHD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21234
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.020"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            156..1589
                     /gene="ccoN"
                     /locus_tag="WM704_000631"
     CDS             156..1589
                     /gene="ccoN"
                     /locus_tag="WM704_000631"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFA9340107.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            1616..2227
                     /gene="ccoO"
                     /locus_tag="WM704_000632"
     CDS             1616..2227
                     /gene="ccoO"
                     /locus_tag="WM704_000632"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFA9340108.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            2232..2402
                     /locus_tag="WM704_000633"
     CDS             2232..2402
                     /locus_tag="WM704_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFA9340109.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            2426..3745
                     /gene="ccoP"
                     /locus_tag="WM704_000634"
     CDS             2426..3745
                     /gene="ccoP"
                     /locus_tag="WM704_000634"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFA9340110.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            4089..5498
                     /locus_tag="WM704_000635"
     CDS             4089..5498
                     /locus_tag="WM704_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9340111.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            5599..8808
                     /gene="recC"
                     /locus_tag="WM704_000636"
     CDS             5599..8808
                     /gene="recC"
                     /locus_tag="WM704_000636"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFA9340112.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSG
                     ELGKLWQQSFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVKSGETEVLAEIAQDRALQLLSEWI
                     VFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(8878..10446)
                     /gene="mtrF"
                     /locus_tag="WM704_000637"
     CDS             complement(8878..10446)
                     /gene="mtrF"
                     /locus_tag="WM704_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFA9340113.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVTSTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            10778..10990
                     /locus_tag="WM704_000638"
     CDS             10778..10990
                     /locus_tag="WM704_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340114.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(11083..11715)
                     /gene="mtrR"
                     /locus_tag="WM704_000639"
     CDS             complement(11083..11715)
                     /gene="mtrR"
                     /locus_tag="WM704_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFA9340115.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQTAGVTR
                     GTLYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIHYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            11963..13201
                     /gene="mtrC"
                     /locus_tag="WM704_000640"
     CDS             11963..13201
                     /gene="mtrC"
                     /locus_tag="WM704_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFA9340116.1"
                     /translation="MAFYASKAMRAAALAASVALALSSCGKADEAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEASLESARAQLATAQATLAKADADLARYKPLVAADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGTKDTVMIVNAQ
                     GGMEPREVTIAQQQGANWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWMPSENQA
                     AAPQSGVQTASEAKPASEAE"
     gene            13213..16416
                     /gene="mtrD"
                     /locus_tag="WM704_000641"
     CDS             13213..16416
                     /gene="mtrD"
                     /locus_tag="WM704_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFA9340117.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPNIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPETDEDL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNIQISAGSIG
                     SLPAVQGQTVTATVTAQGQLSTAEEFGNVILRANTDGSNVYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLLEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKAATKKAMGQISGAVIGITAVLMSV
                     FVPLAMFSGATGNIYKQFALTMAASIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDNWTHGYEGWVAKVLRKTLRMMVVYVGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     MLKDWNERKTPGSDAESVAGKLTGMMMGTLKDGFGIAIVPPPIMELGNGSGLSINLQD
                     RNNTGHAALLAKRNELIQKMRTSGLFNPSTVRAGGLEDAPQLKIDINRAAAAAQGVSF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADGDARMQPADILNLTVPNSSGIAVPL
                     SSIATVSWQMGTEQSVRFNGYPAMELSGSPATGVSTGQAMEAVQKMVDELGSGYSLEW
                     GGQSREEAKGGSQTIALYALAAVAVFLVLAALYESWSIPLAVLLVMPLGLAGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            16469..17872
                     /gene="mtrE"
                     /locus_tag="WM704_000642"
     CDS             16469..17872
                     /gene="mtrE"
                     /locus_tag="WM704_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFA9340118.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSVRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWS
                     FAPSIALPIFTWGTNKANLDAAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     AALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(17984..18173)
                     /locus_tag="WM704_000643"
                     /pseudo
     CDS             complement(17984..18173)
                     /locus_tag="WM704_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(18158..18457)
                     /locus_tag="WM704_000644"
     CDS             complement(18158..18457)
                     /locus_tag="WM704_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFA9340119.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLSPQPD"
     gene            18735..19514
                     /locus_tag="WM704_000645"
     CDS             18735..19514
                     /locus_tag="WM704_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HFA9340120.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(19552..19658)
                     /locus_tag="WM704_000646"
                     /pseudo
     CDS             complement(19552..19658)
                     /locus_tag="WM704_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(19753..21087)
                     /gene="gdhA"
                     /locus_tag="WM704_000647"
     CDS             complement(19753..21087)
                     /gene="gdhA"
                     /locus_tag="WM704_000647"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFA9340121.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCHVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT         5038 a         5251 c         5794 g         5151 t
ORIGIN      
        1 aagggttcgc cggcggcgcg aatgtaattc cgcttggcgt aataaggcag atatgttaaa
       61 tttagaaaaa attacgcttg cccggattgc accttaaaca ggctgcggaa gcggcaggat
      121 aaaaatcttt attcccataa ttcatggaga ctctcatgga cacacaaact tacaactaca
      181 aagtggtgcg ccagttcgcc atcatgactg tagtttgggg gattgtgggc atgttggtcg
      241 gcgttatcgt cgccgcccag ctttttgctc ctgccctcga cttgtctaat atcggacctt
      301 ggttccactt cggccgcctg cgtccgctgc acaccaatgc ggttattttc gcatttggcg
      361 gttgcggtct gatcggcaca tcatactacg ttgtccaacg tacttgtaat acccgtcttt
      421 tcggcggttg gctgccggca tttacctttt ggggttggca ggcggtaatc gttgccgccg
      481 tcgtcagctt ccctatgggt tggacccaag gtaaggaata tgccgaactg gaatggccga
      541 ttgacattct gattactttg gtttgggtgg cttacgccat cgtattcttc ggtacgattg
      601 ccaaacgcaa ggtaaaacac atttacgttg ccaactggtt ctacggcggc tttattttgg
      661 cggtcgcact tttgcacatc gtcaacaata tcagcatccc ggccggtttg atgaagtcat
      721 accccgtcta ttccggtgcg attgatgcta tggttcaatg gtggtacggg cataacgccg
      781 tgggcttctt cctgactgcc ggctttttgg gtatgatgta ctatttcgta cccaaacaag
      841 cagcccgccc cgtttactcc taccgcctgt ccgtcgttca cttctgggcg ttgattttca
      901 cctatatgtg ggcgggtccg caccatcttc actacactgc gctgcctgac tggacgcaat
      961 ctttgggtat ggttctgtcc ttgattttgt tcgcaccctc ttggggcggt atgattaacg
     1021 gcattatgac cttgtccggc gcgtgggaca aactgcgtac agacccgatt cttaaattcc
     1081 tgattgtatc cttgtccttc tacggtatgt ctacctttga aggcccgatg atgtcgatta
     1141 aaacggtcaa tgcattgagc cactatacgg actggaccgt cgcgcacgtt catgcgggtg
     1201 cgttgggctg ggtaggcttt gtaaccatcg gttccgtcta ttacatgatt ccccgtctgt
     1261 tcggcaaaga gcagatgcac agcaccaagc tggtagaggc gcatttctgg attgcgacta
     1321 tcggtgtggt tctgtatatc gccgcgatgt ggattgcagg tgtgatgcag ggtttgatgt
     1381 ggagttctct gaacgatgac ggtacgctga cctactcgtt tgtcgaatcc gtaaaacgca
     1441 ccatgcctta ctacatgatc cgttttgcag gtggtttatt gtatctgagt ggcatgtgca
     1501 ttatggcgta caacgtgtac cgcacagcca tcggtggtaa agcagtcgat gccgaaatcc
     1561 ctgcggtttc ccaaacacag caccactaaa atactaagaa agataggcta ccaaaatgaa
     1621 attacaacaa ttggctgaag aaaaaatcgg cgttctgatt gtgttcacgc tgcttgtagt
     1681 cagtgtcggt ctgttgattg aagttgtgcc cttggccttt accaaggcgg caacacagcc
     1741 ggcgccgggc gtgaagcctt acaatgccct gcaggttgcc ggacgcgata tttacatccg
     1801 tgagggctgt tacaactgcc actctcaaat gattcgtccg ttccgtgcgg aaactgagcg
     1861 ttacggtcat tactctgttg ccggagagtc ggtttacgac catccgttcc aatggggttc
     1921 caaacgtacc ggtcctgatt tggcacgtgt gggcggccgc tattccgacg aatggcaccg
     1981 catccacctg ctgaatcccc gtgatgtcgt gcctgagtcc aatatgccgg cattcccgtg
     2041 gcttgcacgc aataaagtcg atgtcgatgc aaccgttgcc aacatgaagg ctttgcgtaa
     2101 agtaggtact ccttacagtg atgaggaaat tgcgaaagcg cctgaggctt tggcaaacaa
     2161 atccgagctg gatgctgtag tcgcctatct gcaaggattg ggtctggctt tgaaaaacgt
     2221 aaggtaacat catggatatt aacggtattc gcgctctttt cacggtatgg atctttatct
     2281 gtttcctgtt agtactctat atcgtcttca acaggcggaa taagaaaaac tacgatgatg
     2341 ccgcaaacag catttttgct gaaaaccaag atgcgcaaga taagaaaagc gaaaaccgtt
     2401 aatattgtga taacggagca aaacaatgaa cgcaacatcc caatttacca gtaatttctg
     2461 gaatatatat attgcagtta ttgtcttact gagctttatc gctttggctt ggctgctgct
     2521 gtctcaaaat gttgtcaaac gtccgaagaa gggcgaagaa gtacaaacta cgggtcacga
     2581 gtgggacggc attgccgaat acgacaatcc gctgccccgc tggtggtttt ggctgtgtgt
     2641 tttgacgtgg ctgttcggta tcggttatct ggttatgtat ccgggtgtcg gcgactacaa
     2701 aggtctgctg aaatggacca gccataacca atatgaaaaa gaggtcaaaa aagccgatga
     2761 gcaatacggc aaactgtatg ccaagtttgc ggatatgccg attgaaaaag tggcaaaaga
     2821 cccacaggcc aagcaaatcg cccaaaacct gtttaacact tattgtatcc agtgccacgg
     2881 ctctgatgcc aaaggttcta aaggttttcc gaatctgacc gatagcgatt ggttgtgggg
     2941 cggcgatccc gataaaatcc acgagaccat cgaaaaaggc cgtgttgcga ctatgcctgc
     3001 ctggggtcct gctttgggcg aagaaggcgt gaaagacgtt gcccattatg tgatgtccct
     3061 ttcaaaaccc aaaggtcagt atgatgagga acgtgccgcg cgcggacaag ccttgttcag
     3121 cggtccgcct gccaactgtt tcacttgcca cggcgataag ggacaaggta tccaaggttt
     3181 gggtccgaac ctgactgatg acgtgtggtt gtggggcggt acgcaaaaat ccattatcga
     3241 aaccattacc aacggtcgca gcagccaaat gcccgcttgg ggacatttct tggataaaga
     3301 caaactgcat atcatgactg cttatgtatg gggtctttcc aataaagacg gtaaagctcc
     3361 ggtgaaaaaa gccgagcctg caccggcggc agaacccgca ccatctgctc ctgcagaagc
     3421 agcacaagcc gcttctgaag ccaaacctgc cgcagcagaa cctaaagccg aggaaaaagc
     3481 cgcacctgcc gccaaagcgg atggcaaaca ggtttatgaa accgtttgtg ccgcctgcca
     3541 tggcaatgca attccaggta ttccccatgt aggcaccaaa gccgactggg ccgaccgcat
     3601 caaaaaaggt aaggacacgt tgcacaaaca cgcgatcgaa ggttttaata cgatgcccgc
     3661 aaaaggaggt cgcggcgatt tgagcgatga tgaggtcaaa gctgcggttg actatatggt
     3721 caaccagtca ggcggtaagt tctaaccgtt gttttccgac tttgtcggat gcagaaatcc
     3781 ggattcatat cgaatccgga ttttttattt tgaacggaaa tcctgttttt gatttatgcc
     3841 gtctgaaagt ggggattttg atgcttaagc agtcagaccg actgatattt ttgtatggtt
     3901 taaagaatgg tgcagttggg tgtcggacag cattgtttga tgctttcaga cggcatatag
     3961 gtttgtttct tcaaaattga atcttttttc atatgttgca aaaaaattga ttttagtcat
     4021 aaagaattag tctttattgt ttataatcgc gcatcatcac gatagcctta atttcaaaag
     4081 gacgattaat ggatacacaa atcaaaactg aagccggtca gcagaataac cggcattatc
     4141 tgagcgtttg gaggtggcat ttttatgccg gtttattggt tatgcccttc ttgaccctgc
     4201 ttgccgttac aggtttgggt atgctgttgt ttgccaatat taccggtaaa gagggagagc
     4261 gtattcacgt tttccctcag gcagtggtgc aacccctctc tgttcaggca gaagcggcac
     4321 gcggtgccat taatccggag acttcgtccg tcgtccaata tattgcaccg cgtgccgatg
     4381 atatggttgc tgtgttccgt gtcaacaatg agggtaaggc aaccatggtt gcggtcgatc
     4441 cttatacggc aaaagtggtc aacaccatgc cgcgcaatca gggttggtat cacactatgg
     4501 atgaaatcca cggcgatatg atgctcggtg cggcaggcga ttatcttttg gaaacggcag
     4561 cttcactgac cattattatg gttgtcagcg gcttgtacct ttggtgggcg aaacagcgcg
     4621 gcattaaagc gatgctgctg ccgccaaaag gcagggcgcg ttcttggtgg cggaatctgc
     4681 acggcgcgtt tggaacttgg gtatcgttga ttttactgtt gttctgcctg tcgggtattg
     4741 cttgggcagg tatttggggc ggcaagttcg tgcaggcttg gagtcagttc ccggccggca
     4801 aatggggtgt cgaaccgaac cccgtttcag tcgtgccgac ccacggcgag gtattgaatg
     4861 acggcaaggt taaggaagtg ccgtggattt tggagcttac gcctatgcct gtctcaggga
     4921 cgactgtggg tgaaaacggc attaacccca gcgagccgat gacattggaa accgtcgacc
     4981 gtttcgcgcg ggaaatcggt ttcaaagggc gttatcagtt gaatttgccc aaaggcgagg
     5041 acggggtatg gactttgtcg caggattcta tgagttatga catgatcagc ccgtttgccg
     5101 accgcacggt gcatatcgac cagtacagcg gcaagattct tgccgacatc cgttttgacg
     5161 attacaaccc gttcggcaaa tttatggcgg caagcattgc gctgcatatg gggactttgg
     5221 gctggtggag cgtgttggcg aacgtcgtgt tctgccttgc cgtgattttt atcggcatca
     5281 gcggctgcgt gatgtggtgg aaacgccgtc cgtccggcgt ggcgggcatt gttcctccgg
     5341 cgcaaaaaat caaactgccc gtctggtggg cgatggcatt gccgctgctg ttgattgcac
     5401 tgcttttccc gaccgcgctg cttgccattg ccgtgatttg gctgttggat accttgctgc
     5461 tgtcgcggat tcctgtgttg aggaaatggt ttaaatgacc cgatgccgtc tgaagcgttc
     5521 agacggcatt ttgtttgcgg gcggcttgcc cgagaagcta tataattccg catcactgtc
     5581 attttgaaaa agcaagccat gttttacctg taccaatcca accgcctcga atcgctcgct
     5641 gcactgtttg cccgcattca aaaagtcaaa ccgctgaaat gtgccttgca gcccgaacag
     5701 attgttgtgc agagccaggg gatgcgccgc tacctcaaca cctgcctcgc ccgcgatttg
     5761 ggcgtggcgg cgaatttggc gttcagcctg cccgccggcc tgacgtggaa gttgatgaaa
     5821 aaactgattc ccggtattcc ggaactcagc ccgtttgcac ccgaagtcat gcgctggcgg
     5881 ctgctggatt tgttccgcag cgcagaattt caaaacggag cggaatttga agatgtgagg
     5941 aatgtgctgc aagactatct gggcagcgga gaatcggcgg attaccagct tgcgggacag
     6001 cttgcagaca tattcgacca atacctcgtg taccgtcctc agtggataga cgcttggcag
     6061 cagggcagga tactcggttt gggcgacgac gaaatctggc agtccaaact gtggcgttac
     6121 ctcgacgacg gcaggcagag cgcgccgcac cgtgtcgcgt tgtgggaaaa actgcttgcc
     6181 gcgttggaca aggataagct gcccgagcgt tatttcgtgt tcggcatttc cacgatggcg
     6241 ccgatgtatt tgcagctttt gcacaagctg tccgaacatt gcgacgtgtt cgtgttcgca
     6301 ctcaatccga gcgggatgca ttggggcaac gtcatcgaag cggcgcaaat cctcaaaggc
     6361 ggcggcgatc ccgatctgac gcaggcaggg catccgctgc ttgcctcatt gggcaagcag
     6421 gggcgcgact ttttcgactt tttgaacgaa atggaaatag aaggcgaaac gccggtattt
     6481 gaagaaggcg gacgcgatac gcttttgcac gccctgcaaa ccgatattca aaacctgaaa
     6541 atgccgtctg aaaatgtggg aagcgtcaac acgggcgacg gctcgatacg catcgtctcc
     6601 gcacacagcc ctttgcgcga attgcagata ctcaaagaca agctgttgaa aattctgcat
     6661 gaacatcccg attggcagcc gcatgatatc gccgtattaa ccccgaacat cgaaccctat
     6721 acgcctttta tcgaagccgt gttcggacag gcgcagcccg gcgcgcaggc attgccgtat
     6781 tccgtttcgg acgtgaaaat cagccgccgg caaccgtttt tttatgcgtt ggagtgcgtg
     6841 tccgacttgc tggaaagccg ttttgaagtc ggcaaagtgc tcgcgctttt ggaaaccgcg
     6901 cccgtgttgc gccgtttcgg actgaccggg gacgatttac cgcttttgca cgacatggtt
     6961 gccgatttga acgtccgctg gggtttggac ggagaaatgc gcggcggcac ggatcagctg
     7021 tttacctgga agcaggcggc agaccgtatg atattgggct ggatgctgcc cgaaggcggc
     7081 aacccgatgt ggcaggatgt cagcgcgtgg tatgccgacg tgaatcaaac cgccatgttc
     7141 ggacgttttg ccgccttcct cgaaaccctg tcggatattg cccggatatg gcggcaacct
     7201 gcgacggtcg gcgaatgggt ggcgcgttgc cgggatttgc ttgaaacatt gtttcaagcc
     7261 gggcccgatg accaaaaggc agtccaaaac ctcgaaaacg aatgggtcaa atggcaggaa
     7321 gagagcactt tggctgggtt tttcggacag ttgccgcaac aaaccgtcat ccgccatatc
     7381 cgccgcttcc tcgacagcga aagcgaggca ggctttttac gcggcggcat caccttttgc
     7441 agtatggtgc cgatgcggag cctgccgttc aaagtcatct gcctgttggg tttgaacgac
     7501 ggagattttc cccgcaatac caaagccgcc gtattcgacc tggttgccaa acatcccgcc
     7561 aaaggcgacc gcgcccgccg cgacgacgac cgctacctgt tcctcgaagc cctcatcagc
     7621 gcgcgcgaaa tcctgtattt gtcctacatc gggcgcgaca tccgcaaaga cgaagagctt
     7681 gccccgtctt ccctgttggg cgaactcatc gacacagttg ccgccatgac cggcacaaac
     7741 agccgccaac ttgcacaaaa ttggatagaa cagcatccgc tgcaagcctt ctcgcgccga
     7801 tatttccaag aaggcggacg ttcagacggc atattcggca cgcgtaccga ctacgccgcc
     7861 gcgctcggac aaacgcccga accgccgcgc ccttttttcg accaacccgt agaaaacgcc
     7921 gaacccgttg ccgaaatcgg acaggacgaa tttatccgct tctggcgcaa ccccgtcaaa
     7981 gtatggcttc agcagcagct tgcgtggagc gaaccccata tcggcgaagc ctgggagcct
     8041 gccgaaccct tcgagccgca acacgcggag caaatcgccg aaacctatat cggggcgcgc
     8101 cgcgaaggac aagatttttc ccaaaccgcc gcccgtatcg cggcggaaag cctgctgcct
     8161 tccggagaat tgggaaaact ttggcagcag tccttccaaa ccgccgccaa acaaatcgac
     8221 acggcggttt taaacagccc caaactgccc ccgtttccat acgccatacc gtcggacggg
     8281 caaatcctga aaggcagctt gggcaatctg taccgctgcg gtcaggtgtt ttatgcctac
     8341 ggcaaaccca atgcgccgca acgtatcgct tttttgttgg aacacctgat attttgcgcc
     8401 gttatgccgt ctgaagccga aacgcggcaa acctttatcg tcaaatccgg agaaaccgaa
     8461 gtattggcgg aaatcgcgca agacagggca ttgcagctgt tgtcggaatg gattgtattt
     8521 ttcaatatcg ggcaaaaccg cccgctgccg ttttttgcca aaacctcgct tgccgccgcc
     8581 gaagcgtttg cccaaaaaca agattgggaa gccgccctga aaaaagccca aaccgcctat
     8641 tacggcagca aagtcagcaa agggcagaaa gactataccg aagtcgccct cgtgttcggc
     8701 aacgcaagcc aaaacccgct cgaacagccc ctgtttgaaa acctcgcccg cctgctcgcc
     8761 gacacgcttg ccgcagcgga aaagagggaa gggaccggag aagcctgacg ggatggaaag
     8821 gcaaatgccg tctgaaaaca gaaaaaaggt tttcagacgg tattttgttt atcgtgttta
     8881 aggtgcggga tacaaagtgg gcgcgccggg gccgacgggc aggcccaaaa caaataccca
     8941 aatgcagaat aaggcgatcc atgcaattaa gaagaaagcg gaatacggca acatcataga
     9001 aatcagagta cccacgcccg catctttttt gtatttgatt accgtcgcca taatcagccc
     9061 gaaataactc atcatcggcg taataatatt ggtaacggaa tcaccgatgc ggtaagcggc
     9121 ttgaatgact tcgggcgcgt agccggccaa catcagcata gggacgaaaa tcggtgcggt
     9181 taccgcccat tgcgcggaag cggagcctat catcagattg ataaaagcac aaattaaaat
     9241 aaaaccgata aacaacacgc tgccggccaa gccgacttct tttaagaaca ccgccccttt
     9301 aacggcaata tattgcccaa tattcgtcca attaaaaaat gcgacaaact gtgcggcaaa
     9361 aaagatgatg accaaataaa gccccagagt actcatcgat tcggccatcg cattaacgac
     9421 ttcccgttcg ccgcgcaaac ttcgggttat ccggccataa acaatgcccg gcagcgcaaa
     9481 caacaagaaa ataaaaacaa caatcgattt taaaaacggc gaaccggcaa ccaatcctgt
     9541 ttcaggatga cgcaaaatac cgtcggcagg gacgatgctc caagccaata gggcggataa
     9601 ggcaacaaac accacgcctg cccaaattaa tcctttatat tccaaaggcg tgatttcatt
     9661 ggaatgccgg atgtcttttt cttcttgtga caaatctgac tgataagggc ccaattgcgg
     9721 ttcgacgatt ttttcagtaa caaaataacc aatcaaagca atcacaaacg tactggttac
     9781 cataaaaaac cagttggctt cagggcctac gacgtagtcg ggatggatga tttgcgccgc
     9841 ctgttgggta atgcctgcca agagcggatc gagtgtgcct aagaacagat tggccgaata
     9901 accgccggaa acgccggcga aagccgcagc cagaccggcg agaggatggc ggccgagcga
     9961 atgaaagatg acggcggaca aagggattag gacgacatag cccaattcag aagccgtatt
    10021 ggataaaatc cctgtaaaaa caaccataaa agtagtgagt ttgcgtgggg attttgtgag
    10081 caataagcgc attaatgcgg aaatcaagcc cgatttttcc gcaatcccca cgcccaataa
    10141 agaaaccaac accgttccca acggcgcgaa accggtgaaa tttttaacgg tatgcgtcag
    10201 gattttgatc aaaccgtcgg catcgagcag gctgacaacg tgaatcaaac cgtcatcggc
    10261 acgtcctttc gccccaacag gacgcggatc ggggacggat agtccgaaat acgcaccgac
    10321 ggcagaggca atcagcaata acacaatgaa aataataaaa agcgtaaccg ggtgcggcaa
    10381 catattgccc agccattcga ctgtgcgtaa aaatcgtccg ctacgacgcg cgtcggtttg
    10441 actcatctgc ttctccttaa aaaaatatat tgagtgcatt aaatttttga atttaacatg
    10501 aagtgttttg taagcaaact aaaataatcg tatatgtata taaccgtaat agattaaaac
    10561 ggaaaatgcc cacatcgtta ttctcataaa ggcggtaatc cgaaaacttg acgttaaagc
    10621 cttatcagga atgactggaa ctcaaaaaaa cgaattcccg cctgcgcggg aatgacggga
    10681 ttttcggttt ttgtttgcaa gaatgccgga gaaaaatgca gtttacattc gaatggtatt
    10741 gttattaatt gtaaataaat attggaaggt aaatgatatg aaaaatgaaa ttcaaaaaat
    10801 catggataaa tatgatccat ggcatgaaga tgattttgag tcgtatgaag acattgccaa
    10861 agatgtatcg ttgatgacag ataaaacgtt cattgaacat tatttgttag aagtttattc
    10921 agaagaaaac ggacattttg accaagagaa tatccatgcg atgatagggg aaattaaaaa
    10981 tgcaatttag tgaatatgta tttttagaag aatgctttgt gtcattttga tgccgtttga
    11041 aagggtttgt tcgtttcaga cggcattgtt accaaggctt gattatttcc ggcgcaggca
    11101 gggatggttt tccaagttgt ccatcattat cccgatgatg cgcggggcgg ttttgcccaa
    11161 atcgaaactt tcgccggaag agaaccaacg ccaaatcagc ccgtccaacg tcgatttgat
    11221 gaagatgacc gccgtttcct tgtccaaatc gtcagccaaa tcctgatttt ccaccgcttc
    11281 ggtcaaaacg gcggtaattt tctcgcgcca gattgcctga tgcttgcggg caatggcgat
    11341 aacggcggcg ttttgttccg tatgttcgca ctttaaaaac aggatgttgt ggaatttgta
    11401 gtggatgtcg ttgctttgca gccgctcgaa aaagtgcagc agcgtgtggc ggaataccgt
    11461 ccaagaacct ccttcggcat ctgcggcatc ttgcgcgatg cagttttcga tgtcgtcgca
    11521 gatacgttgg aacaacgcgt caaacaagtc ttccttattt ttgaaatgcc aatagagcgt
    11581 gccgcgcgtt acgccggcgg tttgggcgat ttcgttgagc gaggtacgcg caatcccttt
    11641 gcggtaaaag gtttccaagg cggcaagcat caggtgttct ttggttttca aggcttcggt
    11701 tttggttttt ctcataatgg cgttttcgtt ccgtcgcggt ttgacggttg ggattataaa
    11761 aaagactttg tatccgtgta atcgtgtatg tataataacg cccctgaaat tgagacagtt
    11821 tcccagtgcc gaagtgccga acccacctgc tttgcagccc gtttgaatac agggatgccg
    11881 aagcaggcgg ttttgtattt tgaaacggga atttctgtta aatctaaaat cctttaataa
    11941 aatcatacag ataggataga taatggcttt ttatgcttct aaggcgatgc gtgcggccgc
    12001 gttggctgcc tctgtcgcgt tggcactgtc atcttgcggc aaagcagatg aagcggcaca
    12061 gggcgggcag cctgctggtc gggaagctcc ggctcccgtc gtcggcgttg ttaccgtcca
    12121 tccgcaaacc gttgcgttga ccgtcgagtt gccgggacgt ttggaatcgc tgcgtaccgc
    12181 cgatgtccgt gcgcaagttg gcggcattat ccaaaaacgc ctgttccaag aaggcagtta
    12241 tgtccgtgcc ggacagccgc tgtatcagat cgacagttcc acttatgaag caagtctgga
    12301 aagcgcgcgc gcgcaactgg caacggcaca ggcaacgctt gccaaagcgg atgcggattt
    12361 ggcgcgttac aaaccgctgg ttgccgccga tgccatcagt aaacaagagt acgatgcggc
    12421 ggtaacggcg aaacgttctg ccgaggcggg cgttaaagcg gcgcaggcgg cgatcaaatc
    12481 cgccggcatc aatctgaacc gttcgcgcat taccgcgccg atttcgggct ttatcggtca
    12541 gtccaaagtt tccgaaggta cgctgttgaa tgcgggcgat acaactgttt tagccaccat
    12601 ccgccaaacc aatccgatgt atgtgaacgt tacccagtct gcatccgaag tgatgaaact
    12661 gcgccgtcag atagccgagg gcaagctgct ggcggcggat ggtgtgattg cggtcggcat
    12721 caaatttgac gacggcacgg tttatcctga aaaaggccgc ctgctgtttg ccgacccgac
    12781 cgttgacgaa tcgaccggtc agattaccct gcgcgccgcc gtaccgaacg atcagaatat
    12841 cctgatgccc ggcctgtatg tgcgcgtgct gatggatcaa gtggcggtgg ataatgcatt
    12901 catcgttcca cagcaggcgg taacgcgcgg tacgaaagat accgtgatga ttgtgaacgc
    12961 ccaaggcggt atggaaccac gcgaggtaac gattgcacaa cagcagggtg cgaattggat
    13021 tgttacgtca ggtctgaagg acggggacaa ggtggttgtg gaaggcatca gtatcgccgg
    13081 catgacgggt gcgaaaaagg taacgcctaa agaatggatg ccgtctgaaa accaagctgc
    13141 tgcgcctcaa tccggcgttc agacggcatc tgaagccaaa cctgcttctg aagcggaata
    13201 aggaaggcat cgatggctaa attttttatc gaccgcccca ttttcgcgtg ggttatttcg
    13261 attttcatca ttgcggcagg tattttcggc attaaaagcc tgcctgtttc gcaatatccg
    13321 tctgttgccg caccgaatat taccctgagg gctacttatc cgggtgcatc cgcacaagtg
    13381 atggaagaca gcgtactttc cgttatcgag cggaatatga acggcgtgga aggtttggat
    13441 tatatgtcca cttctgccga ttcgagcggc agcggcagcg tgagcctgac ctttacgcct
    13501 gaaaccgacg aagatttggc tcaggtggag gtgcagaaca agctttccga agtattgagc
    13561 acgctgccgg caaccgtcca gcaatacggc gtaaccgtat ccaaggcgcg ttccaatttc
    13621 ctgatgattg tgatgctttc gtcggatgtg cagtcaaccg aagagatgaa cgactacgcg
    13681 cagcgtaata tcgttcccga gttgcagcgt atcgaaggcg tggggcaggt acgcctgttc
    13741 ggcgcgcaac gcgcgatgcg gatttgggtc gatcctaaga aactgcagaa ctacaatttg
    13801 tcgtttgccg atatcggcag cgcgctgtcg gcgcagaata tccagatttc cgcaggttcc
    13861 atcggttcgc ttcccgctgt tcaggggcag actgttacgg ctaccgtaac ggcgcaaggg
    13921 cagttgagta cggcagaaga attcggcaac gtcatcctcc gcgccaatac tgatggttct
    13981 aatgtttacc tgaaggatgt ggcgaaagtc ggattgggta tggaagacta ttcttcctca
    14041 acccgtctga acggggtaaa taccaccggt atggcggtga tgctgtccaa cagcggtaat
    14101 gcgatggcga cggcaaaggc ggttaaagaa cgcatggcga cgttggaaaa atactttcct
    14161 cagggtatga gctggaaaac cccttacgat acttccaaat tcgtcgaaat ttcgattgaa
    14221 aaagtcatcc ataccttgtt agaggcgatg gtgctggttt ttgtcgtgat gtacctcttc
    14281 ctgcaaaaca tccgttatac gctgattccg accatcgtcg taccgatttc cctgttgggc
    14341 ggtttcgcct tcatctctta tatgggcatg tcgattaacg tattaacgat gtttgcgatg
    14401 gttttggtca tcggtatcgt ggtcgatgac gcgattgtgg tggttgaaaa cgtcgagcgc
    14461 attatggcgg gtgaaggcct gccgcctaag gctgcaacca aaaaagcaat gggtcagatt
    14521 tccggtgcgg tcatcggtat tactgctgtt ttgatgtctg tattcgttcc tctggctatg
    14581 ttcagcgggg caacgggcaa tatctataaa cagtttgcac tgactatggc ggcatcaatc
    14641 gcattctctg ccttccttgc gctgacactc acacctgctt tgtgtgccac aatgttgaag
    14701 accatcccga aagggcatca cgaagagaaa aaaggtttct tcggttggtt taacaaaaaa
    14761 ttcgacaact ggacacacgg ctacgaaggc tgggttgcca aagtattgcg caagactttg
    14821 cgcatgatgg ttgtttacgt tggtttggcg gttgtgggcg tattcctgtt tatgcgcctg
    14881 ccgacttcgt tcctgccgac cgaagaccaa ggcttcgtta tggtcagcgt gcaactgcct
    14941 gcgggtgcga ccaaagagcg caccgatgcg acgctggcgc aagtaacgca gttggcaaaa
    15001 agtattcctg aaattgaaaa catcattacc gtttccggct tcagcttttc gggcagcggt
    15061 cagaatatgg caatgggttt tgccatgctc aaagactgga acgagcgtaa aaccccgggc
    15121 agcgatgcgg aatcggttgc aggcaaattg accggtatga tgatggggac gcttaaagac
    15181 ggtttcggca tcgccatcgt tccgcctccg attatggagt tgggcaacgg ttccggtttg
    15241 agcatcaacc tgcaagaccg caacaatacc ggtcatgccg cattgctggc caaacgcaac
    15301 gagttgattc agaaaatgcg taccagcggt ttgtttaacc caagtaccgt ccgtgccggc
    15361 ggtttggaag acgcgccgca gttgaaaatc gacatcaacc gtgccgcggc ggcggcgcaa
    15421 ggcgtttcgt ttgccgacat ccgcaccgca ttggcaagcg cgctgagttc gtcttatgtc
    15481 agcgacttcc cgaaccaagg ccgtctgcag cgcgtgatgg tgcaggcaga cggggatgcc
    15541 cgtatgcagc ctgccgacat cttgaatctg actgtgccga atagctcggg cattgccgtt
    15601 ccgctttctt cgattgccac cgtttcttgg cagatgggta cagaacagag cgttcgtttc
    15661 aacggttatc ccgctatgga actgtccggt tcgcccgcaa ccggtgtttc caccggtcag
    15721 gccatggagg cagttcaaaa aatggttgac gaattgggca gcggttacag cctggagtgg
    15781 ggcggacagt cgcgcgaaga ggcaaaaggc ggttcgcaaa ccattgccct ttacgcgctt
    15841 gccgccgttg ccgtcttctt ggtgcttgca gcactttatg aaagctggtc tattccgctg
    15901 gccgttctgc tggtgatgcc tttgggcttg gcgggcgcgg ctgcgggcgt aaccgggcgt
    15961 aacctgtttg aaggattgtt gggcagcgtt ccctcgtttg ccaacgacat ctacttccaa
    16021 gtcggtttcg ttaccgtgat gggtttgagt gcgaaaaacg cgattttgat tattgaattt
    16081 gccaaagacc ttcaagcgca agggaaaagc gcggtcgaag ccgctttgga agcggcccgc
    16141 ctgcgtttcc gtccgattat tatgacttcg tttgccttta ttttgggcgt ggttcccctg
    16201 tatattgccg gtggtgcaag ttctgccagc cagcgcgcca tcggcacgac cgtattctgg
    16261 gggatgctca tcggcacgct tttatccgtg ttccttgttc cgcttttcta tgtggtggtg
    16321 cgcaaattct tcaaagaaac cgcgcacgag cacgaaatgg cggtcagaca tgcatcaaaa
    16381 gcgggtataa ccggttcgga cgataaacaa tattaagcaa aataccgtct gagaaccgcc
    16441 ggttttcaga cggcatcagg acttttttat gaatactaca ttgaaaacta ccttgacctc
    16501 tgttgcagca gcctttgcat tgtctgcctg caccatgatt cctcaatacg agcagcccaa
    16561 agtcgaagtt gcggaaacct tccaaaacga cacatcggtt tcttccgtcc gcgcggttga
    16621 tttgggttgg catgactatt ttgccgaccc gcgcctgcaa aagctgatcg acatcgcact
    16681 cgaacgcaat accagtttgc gtacagccgt attgaacagc gaaatctacc gcaaacaata
    16741 catgatcgag cgcaacaacc ttctgcccac gcttgccgcc aatgcgaacg gctcgcgcca
    16801 aggcagtttg agcggcggca atgtcagcag cagctacaat gtcggactgg gtgcggcatc
    16861 ttacgaactc gacctgttcg gacgcgtccg cagcagcagc gaggcggcac tgcaaggcta
    16921 ttttgccagc accgccaacc gcgatgcggc acatttgagc ctgattgcca ccgttgccaa
    16981 agcctatttt aacgagcgtt atgccgaaga agcgatgtct ttggcgcagc gtgttttgaa
    17041 aacgcgcgag gaaacctaca agctgtccga attgcggtat aaggcaggcg taatttccgc
    17101 cgtcgccctg cgtcagcagg aagccctgat cgaatccgcc aaagccgatt atgcccatgc
    17161 cgcgcgcagc cgcgagcagg cgcgcaatgc cttggcaacc ctgattaacc aaccgatacc
    17221 cgacgacctg cccgccggtt tgccgttgga caagcagttt tttgttgaga agctgccggc
    17281 cggtttgagt tccgaagtat tgctcgaccg tcccgacata cgcgccgccg aacacgcgct
    17341 caaacaggca aacgccaata tcggtgcggc acgcgccgcc tttttcccat ccatccgcct
    17401 gaccggaagc gtcggtacgg gttctgccga attgggcggg ctgttcaaaa gcggcacggg
    17461 cgtttggtcg tttgcgccgt ctattgccct gccgattttt acctggggta cgaacaaagc
    17521 caaccttgat gcagccaagc tgcgccaaca ggcacaaatc gttgcctatg aatccgccgt
    17581 acaatccgcc tttcaagacg tggcaaacgc attggcggcg cgcgagcagc tggataaagc
    17641 ctatgccgct ttaagcaaac aaagccgcgc ctctaaagaa gcgttgcgct tggtcggcct
    17701 gcgttacaaa cacggcgtat ccggcgcgct cgacttgctc gatgcggaac gcagcagcta
    17761 ttcggcggag ggtgcggctt tgtcggcaca actgacccgt gccgaaaacc ttgccgattt
    17821 gtacaaggcg ctcggcggcg gattgaaacg ggatacccaa accggcaaat aaccgccagg
    17881 gcaatcaaat gccgtctgaa acgataaggt ttcagacggc atttctttct ttaaaacggt
    17941 ttccggtgcc ggtctgcctt ttaagacggc aggccgtatc tgtttagaag gtatgtttga
    18001 cggttgcggt caggctgcgc ggcgtgccgt aaacatgaat atccggcatc gtgcggtagc
    18061 gtttgttgaa cacgttttca aagtccaaac cgatgcgggt ggatttaccg attttgtaat
    18121 gcgcggtcaa gtccagtgta gcgtatgggg cgttgggtca atccggctgc ggcgacaggg
    18181 aagaaaccgc catacccgtc ggcatcccga agccgaatac ggcaacggca agcgcaatca
    18241 gcagcgtata aagccccgcc tgatagcctt tcagcttcag gacggtcagc gcggcaaaga
    18301 aaaagacgat gggtaagagt gcggcggcgg cagtcagata caggctgccg ccgattgccg
    18361 tgtagttttg aacccaagtt tccataattg aacatctccg aaaatatttt tctaatcgtc
    18421 ggcaatagtg gtcaaaccaa ttaaagcaac gttgcattac tttacgaaac tttaatattc
    18481 aggtcaatat atttttgggc ggttcggcag atttgaatcg gagctttcat ttaaatcccg
    18541 ttaaaacaaa tatttgcatg aacaaaaatt gtagtttggt gtagtttttt cctgtgtttc
    18601 gggggcggtg cgaggtaagt gccgttgacg gcgggaacgg cgtgtcatta gaatccgccc
    18661 tgattggcca gtccaatttg atgtttgatg tatttcggaa gggttctgca gggaatcttt
    18721 ataaaggagg agtgatgaaa ctggtaaggc ctcagaaaat cagcgatcag gtattgtcgg
    18781 tattggaagg ccgtatcgtg gaaggggttt acgcggaagg gggcaagatt ccgcccgaac
    18841 gcgttttggc ggaagagttc ggcgtttcgc gtccgtcggt ccggtcggca ttgaatattt
    18901 tggttgcccg tcagattttg gaagcgaggc agggcgatgg ttattatgtt tcggtcaagc
    18961 cgcagcagga ttttttgcaa agttggcagg agcttttggg caaacattcc aattgggaac
    19021 aggatgtttt tgatttcagc tgccacatcg agggctgtat ggcggcattg gcggcagaac
    19081 gccggacgga tgccgatttg aagcggattc ggttttggct tgaaaagttt gaggaagcgt
    19141 gcggaagcgg caatctggaa catcagggcg aagccgatgt cagcttccat cagacgattg
    19201 cggatgcggc gcacaatttg ttgttcagcc atttgtcggg cggtttgttg aaaatgctgt
    19261 accggcagac gcgcagcagc cttatttatc tgaatcagga agaagatccg cgtccgaaac
    19321 tgatggcgca gcaccgcgtg ctgtatgagg cgatatcgaa ccgccggccg ggcgaggctt
    19381 cggaggcggc aaaggctcat ttgaattatg tggcgagcag catattgaaa gacagggaat
    19441 atcagagccg taaccgccac gcggatactt tggcgcaaaa cgatttgaag cgcgtgcagg
    19501 attgggaggt atgaataccg ttcagacggt ctggcatatt cggatgtatc ggtatagtga
    19561 actaaattta aaccagtacg gcgttgcctc gccttgccgt actatttgta ctgtctgcgg
    19621 ctcgccgcct tgtcctgatt taaatttaat tcactatagt attacccgat tcgaacgggc
    19681 ggataatgaa atgccgtctg aacagcattt gcggttcgga cggcattttg tttgcggggc
    19741 ggaggcgttt gcttagaagc cttgcgccag catcgcatcg gcaactttga cgaagccggc
    19801 gatgttcgca ccgttgacgt agttcacttt gtcgccgact ttgccgtatt tcaggcagga
    19861 ctcgtggatg ctttgcatga tgccgaacag tcggctgtcc acttcttcgc gcgcccaaga
    19921 caggcggatg gcgttttggc tcatttccaa gcccgaagtt gccacgccgc cggcgttgga
    19981 ggctttgccc ggagcgtaga ggatgccggc tttgataaat tgctcgaccg cgcccaaagt
    20041 cgacggcata ttcgcgcctt cggcaacgac gtggcagccg tttgccaaca gggttttggc
    20101 ggcttcttcg tccaattcgt tctgggtcgc gcagggcagg gcgatttcgg cggcaacgcc
    20161 ccacggtttt tggttttcaa agtattgcaa accttgctct ttggcgtagg tggcaacgcg
    20221 ttcgcggcgg acttctttca attcgatcaa ggcggcgagt tgcgcttcgg tcataccgat
    20281 gtcggggaag aggacgaagc cgtcggagtc ggagacggtc agcactttcg cacccagttg
    20341 gatggctttt tcagcggcgt attgcgccac gttgccggaa ccggaaatca gaacgcgttt
    20401 gccttcaaaa ctatcgttgc gggtttgcag catcgcttgg gcgaaataga cgcagccgta
    20461 gccggtcgcc tcggggcgga tgaggctgcc accccattcc aaacctttgc cggtcaggac
    20521 ggaagtgaat tcattgcgga tttttttgta ttgtccgaac aggtagccga tttcgcgtcc
    20581 gcctacgccg atgtcgccgg ccggaacatc ggtgtccgcg ccgatgtggc ggtagagttc
    20641 ggtcataaag gcttggcaga agcgcattac ttcggcatcg gatttgcctt tggggtcgaa
    20701 gtcggaaccg cctttgccgc cgcccatagg cagggtggtc aaggcgtttt tgaacacttg
    20761 ttcaaaagcg aggaatttca acacgcccaa atcgacggtc gggtggaagc gcaggccgcc
    20821 tttgtaaggg ccgatggcgg aactcatttg cacgcggtag ccgcggttga cttggacttg
    20881 tcctttgtcg tcctgccagg ttacgcggaa catcacgacg cgttcgggtt cgacgatgcg
    20941 ttccagcagg ctttgctggg tgtatttcgg attttttgcc aaaaacggat caaggctcat
    21001 gaagacttct tcaaccgcct gatggaacgg ctcctgattg gggttgcgtt gtttgaggtt
    21061 ggcaaacagg gtgttcaggt cagtcattcg gatactcctt ttggttgata ttgtactaat
    21121 gggaaaacaa atttgtagtt ggcaatataa acctattttt ttctgatgcc aagcctaatt
    21181 ttgcacaaat tgttgacatt atttttccca tattttcgat atatgatgtt tatt
//
LOCUS       DAWTHD010000021        21043 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWTHD010000021 DAWTHD010000000
VERSION     DAWTHD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21043
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.021"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..743)
                     /locus_tag="WM704_000648"
     CDS             complement(<1..743)
                     /locus_tag="WM704_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9340122.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLNQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTGKQDAK
                     LIAQYCRSAQESELVKRQKPTDEQYRLSRMIAAYAQIKSECAAMKNRHHAAKDEEAAK
                     AYAQIIKAMNEQLEVLKEKIKEQTEKPNCKEGVKRLETIPAIGRMTAAVLFHHLTSSK
                     FETSNKFAAFAGLSPQQKESGTSVRGKGKL"
     gene            751..1397
                     /locus_tag="WM704_000649"
                     /pseudo
     CDS             751..1397
                     /locus_tag="WM704_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(1469..3214)
                     /gene="recD"
                     /locus_tag="WM704_000650"
     CDS             complement(1469..3214)
                     /gene="recD"
                     /locus_tag="WM704_000650"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFA9340123.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(3281..3976)
                     /gene="lolD"
                     /locus_tag="WM704_000651"
     CDS             complement(3281..3976)
                     /gene="lolD"
                     /locus_tag="WM704_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFA9340124.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3969..5216)
                     /locus_tag="WM704_000652"
     CDS             complement(3969..5216)
                     /locus_tag="WM704_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFA9340125.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            5442..5720
                     /locus_tag="WM704_000653"
     CDS             5442..5720
                     /locus_tag="WM704_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340126.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5776..6393)
                     /gene="recR"
                     /locus_tag="WM704_000654"
     CDS             complement(5776..6393)
                     /gene="recR"
                     /locus_tag="WM704_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HFA9340127.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(6460..7998)
                     /locus_tag="WM704_000655"
     CDS             complement(6460..7998)
                     /locus_tag="WM704_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFA9340128.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(8047..8421)
                     /locus_tag="WM704_000656"
     CDS             complement(8047..8421)
                     /locus_tag="WM704_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFA9340129.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            8571..10199
                     /locus_tag="WM704_000657"
     CDS             8571..10199
                     /locus_tag="WM704_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFA9340130.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            10278..11510
                     /locus_tag="WM704_000658"
     CDS             10278..11510
                     /locus_tag="WM704_000658"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFA9340131.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11929..12237
                     /locus_tag="WM704_000659"
     CDS             11929..12237
                     /locus_tag="WM704_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340132.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            12319..13350
                     /gene="ruvB"
                     /locus_tag="WM704_000660"
     CDS             12319..13350
                     /gene="ruvB"
                     /locus_tag="WM704_000660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFA9340133.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(13595..14278)
                     /gene="rpe"
                     /locus_tag="WM704_000661"
     CDS             complement(13595..14278)
                     /gene="rpe"
                     /locus_tag="WM704_000661"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFA9340134.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            14424..14858
                     /locus_tag="WM704_000662"
     CDS             14424..14858
                     /locus_tag="WM704_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFA9340135.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14871..15734)
                     /locus_tag="WM704_000663"
     CDS             complement(14871..15734)
                     /locus_tag="WM704_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9340136.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15742..16359)
                     /locus_tag="WM704_000664"
     CDS             complement(15742..16359)
                     /locus_tag="WM704_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFA9340137.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(16522..17136)
                     /locus_tag="WM704_000665"
     CDS             complement(16522..17136)
                     /locus_tag="WM704_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFA9340138.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            17343..19112
                     /locus_tag="WM704_000666"
     CDS             17343..19112
                     /locus_tag="WM704_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HFA9340139.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            19109..19765
                     /locus_tag="WM704_000667"
     CDS             19109..19765
                     /locus_tag="WM704_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFA9340140.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19921..20829)
                     /locus_tag="WM704_000668"
     CDS             complement(19921..20829)
                     /locus_tag="WM704_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9340141.1"
                     /translation="MRFPLPITNAVLKITNGAMEFKSEDIGTIKLTKATFHLKKDTSL
                     TTPEGTTLLSGGTLTLSNTGISLSGTTSVFEKGTFTNGGIITLANQSYADKLTIEGNY
                     VGKDGVLKVNTEWNSPGDDQGGNSQSDLLEITGDASGKTTVISVGKDGKENIIDGSIG
                     ELSDRYKRSAAVVKVLGQDKGAETGKLNIEDAKHTYTMRDTFSGTAKTTGAGELQLVS
                     HKDEAGATEYFWTLTTPNQDKTIITPSAPAYALVPRQNLESGYAMLDTLHQRRGENQT
                     LSRDRQGNYRQDAEATDIKATKAPWV"
BASE COUNT         4822 a         6052 c         5239 g         4930 t
ORIGIN      
        1 agtttgcctt ttccccttac gcttgtcccg gattcttttt gttgcgggct taagcccgcg
       61 aatgctgcaa atttgtttga tgtttcaaat ttcgaagatg ttagatgatg aaacaatacg
      121 gctgcggtca ttctgcctat tgccggtatg gtttcaagac gcttcacgcc ttccttgcag
      181 ttaggcttct ccgtctgctc ttttatcttc tcctttaaaa cttcaagctg ttcattcatg
      241 gctttgatga tttgcgcata tgctttggcc gcttcttcat cttttgccgc gtgatgacgg
      301 tttttcattg ccgcgcattc gcttttgatt tgcgcgtatg ctgcgatcat ccgtgaaagc
      361 ctgtattgct cgtccgtagg cttctgcctc tttacaagct cgctttcctg cgccgaccgg
      421 caatactgcg ctatcagttt tgcatcctgt ttgcctgttt tggtgcgttt gaacctgctt
      481 tctgcatact tgcttatttt cagcggattc actacgtaaa cgctgtaata ctgcgcgaag
      541 tagtcggcaa cttcttcata ataattgccg gttgcctcca tgcagatatg cagattctga
      601 catcccaagc ttttcaaccg gtccgaaaac tgatttaaac cttttgaatc gttgtcaaac
      661 tttgccgaat attcggcatt gccgacaatg gccgttgcgt caaatgtcag cttggatatg
      721 tccaatccta cagtgttacg caatatataa ggggttgccg ttccgcagtt gggcggcagc
      781 atacacgttc ctttcggcgt attctcgccg ttgcgcggcg aatatctcga cctgcttgcc
      841 catgcgccgt caacgggttt tcagacggca ttcgatatcg ggacgggctc gggcgtactt
      901 gccgccattt tggcgaaaca gggcattcct tccgtcatcg gcacggatac caatccgagg
      961 gcggttgcct gcgcccgtgc caatattgcc cgtttgggtt ttgaaaaaca ggttgaaatc
     1021 cgcgaaaccg atttatttcc cgaagggttt gccgacctga ttgtctgcaa tccgccccgg
     1081 cttcccgcca agccgacttc cgccgtcgaa tccgcgttat acgaccccga atctgcgatg
     1141 ctggctgcgt ttttgcgtga tgcgccaaac atctgaatcc cgacggagaa atccgtctta
     1201 tcatttccga cctcgccgtg catttggggc tgcggtctgc cgactttttg gaaaaggcat
     1261 ttatccgggc gggtttgcgt gttgaggatg ttttgaaaac caaaccggtt cacaaaaaag
     1321 ctgccgattc gaacgatccg ctggcttttg cccgaaacag ggaaactact ttcctgtgcc
     1381 gtttgaaaaa ggcataaggg gcggtggcgt gcttccgacg gtaggcatcg gaacgcccgc
     1441 gccgccggca cggcatcatt cgggcggatt attcttgtga aaatacccgc tcaagcatac
     1501 tgcccaacgc cgtctgacgg actttgacgg tgtttaccgc ctgacaaaat gtctttttgc
     1561 cgccgaaaaa tacgaatttt tccctggcgc gggtaatggc ggtatataac agctccttac
     1621 tcaatccgga caatgcatcg tccccttcgt ccgaaggtgc gtcggaaggc ggcagcagcc
     1681 agacttcccg atattccgaa ccttggcttt tgtggacggt catggcgaat gcgggttcaa
     1741 attcgggcag gcagcttacc gctacctttt taaatccgtc cgcatcggca aaataggcgg
     1801 caaggctgcc ctgccgtccg acatcttcca taatcagtcc gatgtcgccg ttgaacagct
     1861 caagcgcgta gtcgttctgt ctgatcataa tcggctctcc ggcgaaatag gccaaatgtt
     1921 ccggtatgtt cattttgcgg cgtacatagc tgcaataggc ttcgttgaag tcttccgcat
     1981 cctgccgcca agctgccaga accacgatat ccgagatgcc tgcgtatgcg gcttcgatat
     2041 tgccgtcttt taccgcctgc caataggctt tgtgcgcccg gtacaacctt tcgacccgag
     2101 cattcggact gcattccgaa tgttccagtt cgtccggaaa ccggtcaaac aatgcccacg
     2161 ccccttcatc gcccgatacg gcggcacggg caaggcagcc gatgccgctg ttgtcgccga
     2221 agcggtggct gaacgacaga tgggcggtgt tttgcgccaa cacgggcgga tttgcgctga
     2281 cgctgaaacc gtgttccgga aggaaaccgg ccagcctttg gtgcgtttct ccgtccaaaa
     2341 cggttttttg cgacaaaacc gacagcaccg ccccgattcc gacggacggg agctggtttt
     2401 catcgcccag cagaatcacg cgcgcgccgg ttttgaccgc tttcaaaagt tgcagcatca
     2461 atgcggtatc caacatggag gcttcatcga caatcagcac gtcgaacggc agcggacgga
     2521 tatggtcgaa cgccgcctgc attttgggcg ggctcagctt aagcagtcgg tggacggttt
     2581 gcccttccag tttgagcaaa tggcggcgga cggcctccgg cgcgtcaaaa ccgttgattg
     2641 cacggtgcag cgcgcgcgcc atatgtgccg ccgctttgcc cgtcggtgcg gcaagcgcga
     2701 tatggggaag attttcgttt tcaccgcaaa tcagtgccag cagtttggca accgttgtcg
     2761 ttttgcccgt tcccggccct ccggtaatca ccataaaaaa ctgcaacagt gccaaggcgg
     2821 ccgcatcgcg ctgtccttcg cttcccgcgc cttgaaacca ttttgcgagg ttttgccgcg
     2881 cgcctgccgc gtcgggggcg gacgtgccgg ctgccgccaa gcgttttatt tcggcagcca
     2941 aatcgtattc caactgccac atcctgccca aaaacagcct tctgccttcc aaaatcaaag
     3001 gcgcggcgga tgttccgacc acgggtgcga gtgccgacag cgcgtcagcc tcgtccccgc
     3061 tcaaacggat aaacgaatga ccgttttgca atgcctgaaa caggcgttcg gtacagtttg
     3121 caagcacttc gttgcccgaa cccgcatagc gttccaaaaa acggattgcc gcccttgccg
     3181 ccgcttgggc aaattcatct gtctgcagtt ccatcgtatt ccttgtcgaa atgccgtctg
     3241 aaggcacggg gcttcagacg gtgcgtgttg tttcggctgt ttaggcgttt gcgccctgtc
     3301 tggggtgcag gctgccgtct ttcatgacca tcacgcgctc gaagcggccg gcgagttcgt
     3361 cgtcgtgcgt tacgaccacc agccccgttc ccaattccgt tttcagttcc agcatcatat
     3421 ccaaaacatt ccttgcgttt gcccggtcga ggttaccggt cggttcgtcg gcaagcaggc
     3481 atttgggttg cgtaaccaag gcgcgcgcaa tggcggcacg ctggcgttcg ccgccggaca
     3541 attcgcccgc acggtgcgtc gaacggtgtt tcagtccgac cttgtcgagc atcgccattg
     3601 ccgtttctac cgcctcttta cggctttttt tgccgatcag aagcggcatc atcacgtttt
     3661 ccagtgccga aaattcaggc agaagatgat ggaactggta cacgaaaccg agatggcggt
     3721 tgcgcaaatc gcccaaacgc cgctggttta aggcacgcaa atcctcgccc atcagcagca
     3781 ccctgccttc agacggcata tccagcccgc ccaaaatatg cagcagcgtc gatttgccgc
     3841 tgcccgaaga gccgatgatg ccggtgcttt cccccgtgcg gatttccaaa tccaagccgt
     3901 acagcaccgg aacgtccaaa ccgccgtcac ggtagcgttt gcccacgcct tcgcatttca
     3961 aaatcaaatc actcataacg caaagcctcc gccggttggg tttttgacgc gcgccagctc
     4021 gggtacagtg tcgccacgaa agacagcccc aaggaaatgc aggcaatcag ggcaacgtcg
     4081 cccatatcga catcactggg caggtagtcg ataaaataaa cctgcgaatt gatgaggtgg
     4141 acaccgagca ggttttcaaa aaacgccacg accctgccga cgttccaacc ccaaagcacg
     4201 ccgcagacca cacccgccag cgtgccgaaa aagcctgaaa acgcgccctg caccataaaa
     4261 atcttcatca cgccggcagg ggaaagaccc aaagtccgca aaatcgcaat gtccgcctgc
     4321 ttttccgtaa ccgccatcac caaggaagag acaaggttga acgccgccac ggcgataatc
     4381 agcgtcagga taatgaacat catccgtttt tccagttcga ccgcttcaaa atagctgcgg
     4441 ttgctgtacg tccaatcgcg cacccaaacc gtgtcccttt gcgcctccgg aatcagcgtt
     4501 gccgtcaagg cgggggcgtt ttgcggatcg gcaagcttca gccgcagccc cgcaacttcc
     4561 ttatccaagc ggtacagtac gcgcgcgtct tggatatgcg tcatcgccaa tgagttgtcc
     4621 acttcgtaaa cacccgtctt gaccaggccg accacggtaa actgcttcag gcgcggcacg
     4681 actccggcag gggtaacgtt gccctccggc gtaattaccg taaccttgcc gccgacttcc
     4741 gcccccaaag cctccgccaa accgacaccg aggataatgt caaactcgcc cggaatcagg
     4801 tcttcaaact tgcctgccgg cattttgtcg ccgtattcca ccactttgcg ctcttcagac
     4861 ggcaaaatgc cgcgtatctg aacgcccctg atttcgcccg cgtttgccag caatgcctga
     4921 ttggaaacat agggtgcggc agccaaaatg cctttgcggt tttcggcaaa ccgaagcagg
     4981 ttgcgccaat ccgtatccgt attgtcgata tagccgattt cggcgtgcgg cgcgacattc
     5041 aggagctgcc cgcgtatttc tttctgaaag ccgttcataa ccgacaagac gacaatcagc
     5101 gcggttacgc ccaaggcgat tccggcaatc gaaaccatcg tgataaacga cataaagccg
     5161 ttgcgctttt tcgccctgag atacctcaag cctatccaag cctctagaga aaacataacg
     5221 ctaccttaaa aatgtctgca aacgtgccgc cccggacggc ggtttggggg aggcggcaaa
     5281 agtttattgt accgtaaaac cccggcagcg tccgaacggc aggctgtgta aataaagacg
     5341 cggtatgtgg caaattcgga aaaacaggac ggaaaacttg cacaacgcgc cgaactaccc
     5401 tatccttctc ttgaaacaaa accttttctt taaggaaaac aatgaatatc aggaaaatct
     5461 ccgctttgtg tgctgttgcc gtttttactg tttcgacagc ctatgccaaa gaaatcaaaa
     5521 tcgatgccaa caacacgcct tattccgaag ccgacgcgca aaagctggcg gcaacggcag
     5581 tcggtatggg cgttaaggaa cctgtcagcc tgaacggcgg cagcggcagc attaccgttt
     5641 ccggcagcag cgcggcgcag tgcgtcttta cggtcggcag cgggggcact ttgcagattc
     5701 aagggctcaa ctgcaagtaa accgcccgaa aaaatgccgt ctgaaggctt cagacggcat
     5761 tttgcattgg cggcgttacg ccccgccttc tttaatcaga cgccgttcgt acaccgcctg
     5821 cgccagcgtt cccgcatcga catattccaa ttcgccgccc aatgggatgc cctgcgacag
     5881 cctgctgacc ttgtaaggca ggtttttgaa aaactcggac aggacatacg ctgtggcatt
     5941 gccttctgcg gtaaaggcgg tcgcgataat gatttcttcg atttccccgc cgtccagccg
     6001 ttgcgccagc ctgtccaatg cgatggcgga tacgtccatt cccaatgccg tattgatttg
     6061 ccccatcagg acgaaataca gcccgtcgtg gcagtttgcc gcttctatat tcgacacgtc
     6121 ggcaggcata tgcaccacca tcagccgccg cccgtcgcgt gtttcatcgg cacaaatatc
     6181 gcacaatccg ccttcgcaaa atgtattgca ccttgcgcaa tggcgaacct gcctcaatgc
     6241 cgtctgtaag gcatccacca gctcttcagc ctctttgcgc ttttgctgca acagatggta
     6301 cgccatccgc tgtgccgatt tcggcccgac gttgggcaat actttcagcg caccgatcag
     6361 gcgttggaat gcatcctgtt ttttggaatt catcatactc cgccatatgg gaaaacggtc
     6421 ggaatattcc gaccgttatg ttgtcaacaa aagtgtcaat tactgaccat cgccgttgtc
     6481 aaccgattgt gctcctttag tctgtttgat ttttccgttg aaatagcgga tcagcaggtc
     6541 gaaagtattg gcagactgct gttgcgccaa agcctgtttc gcaggaggaa gctgtgcggc
     6601 aatatcctcc ggaggcgtga ctgcctgcac ctcgacaatc acgggtgccg gcagaccggt
     6661 cagtctgaca taggcgggtt tgccgtttgc cggttttgct ttcagcagtt ccgcataagc
     6721 ctcgggcggc atggactgcc ttgcctgctg cgcgcccaaa acggacactt ccgaccattt
     6781 cacgtcaact gccttgccgc cgttcagttg ggtaagcact tcttttgcct tgttttcggc
     6841 aagtttggcg gcttcggtac ggatataggc ctgacgcacc gcatctttgg cttcttcaaa
     6901 cagtaggttt ttttcttcgc ggacttcttt ggcgcggacg acccacgcgg tttcgctgtt
     6961 gatggtcagc acttcggaat tgtgtttttt cttcaatacg tcgtcgctga atacggcatt
     7021 gattaggttt tcgggcatgc cggacatttg tgcgtcctgc ctgctcagcc aagtttcttg
     7081 ggtttccact ttcaaaccgc tgtttttggc ggcttcggca agcgaggagg gatgattgaa
     7141 cgcatcgtcg cccagctttt cttttgcctt gttgaagtcg gcaaccgcct ttttcatttt
     7201 caattcgttt tcgacggcgg ctttttcctg ctcgaaagaa ggtttggctt catgtgccgg
     7261 caaacgcgcc acgcgctctt caaacgcatt tttcacttcc gtttcactga cggtctgctt
     7321 gtctgcaaaa tccttcagat tcaaggcgac atattccaat ttgaccgcct gcggcagcag
     7381 atagtctttt ttgttcgcat tataaaattt ctgcaaatcg gcttcagacg ctttgacttg
     7441 ggcgatgaac tcgtcggggt tgaaagtgtg cgaacggatg gtgcggttga cctgcgtcag
     7501 cctgatcagc tgttccgcct gcgcgtcgcc gaccaatacg ccgttttgga cgaggcttac
     7561 caaattctgc aaggcaaact gatcgcggat ttcttcgaca aactggtctt cagacatatg
     7621 gcgttgcgac aggtattgac tcaaaagcgc gtgactgaat ttgccgtttg cgtcgtggaa
     7681 attgggatcg tccacaatca tctgcttgat ttgttcggaa gaaaccgaaa tgcccatcag
     7741 cttcgcaccc tgtttcaggt aggcgcgttg cagcagggat tggaacaccg cgtcgcgcca
     7801 agggctgccg ccgtccgcct gctcgttctg catggcgttg ttgattgagt gctcgctgat
     7861 tttttcgtcg cccacttgga cgatgtagtc ggcgcccgga tgggaaaccg tgctgacgcc
     7921 gaagccgaca aaagttaatg caatcaggcc taataagact tgggcgggtg ttctgtattt
     7981 ttcgatggaa tggaacatat tttcagattg ggaatgggaa attcaagtcg ggtattgtaa
     8041 cggtttttat ccctgtctgc acggggcttg ccggttgaag atgccgtcgt aggtttcttc
     8101 gctgaagccg acgtaaaccc tgctgccgca ctccaatacg ggacgcttga tcaggctcgg
     8161 catttcggac atcagtttga cggcctccgc cgtcgaggac agggcttttt gctgtgtttc
     8221 ggcatcgagt ttgcgccagc ttgtcccgcg tttgttgaac agggttgcca aaggcacttg
     8281 ttccagccac gagcagattt ccgcttcaga cggcatctgt tttttgaaat cccgaaaccc
     8341 aaactccaag ccgtatccgg caagcctgtt tttggctttt ttgactgtgt cgcaatttgg
     8401 aatgccgtga aggattatca tttggaaacc ttttgcctga aataataaaa ccgatatttt
     8461 actataagtg tctgaaaatt tgcccgtctg tttcagacgg cggggcggtt atgttacaat
     8521 ccgaaaattc gaaaaattta atctcttgtt caataaaggt tttacccata atgatttcta
     8581 ccaacggcat taccatgcag ttcggcgcga agccgctgtt tgaaaacgta tccgtaaaat
     8641 tcggcgaagg caaccgctac ggcttgatcg gcgcgaacgg ttcgggcaaa tccaccttca
     8701 tgaaaatcct cggcggcgat ttggaacaga ccgccggcga agtggcgatt gaaaacggcg
     8761 tgcgtttggg taaattgcgc caagaccagt ttgcctacga agacatgcgc gtgctggacg
     8821 tggtgatgat ggggcatacc gaaatgtggg cggcgatgac cgagcgcgat gcgatttacg
     8881 ccaatcccga agccaccgaa gacgactaca tgaaagccgc cgaactggaa gccaagttcg
     8941 ccgaatacga cggctacacc gccgaagcgc gcgccgccga attattaagc ggcgtgggca
     9001 tttccgaaga tttgcacaat gcgaaaatgg cggaagtcgc cccgggcttc aaactgcgcg
     9061 tattgctggc gcaggcgctg ttctccaagc cggatgtatt gctcttggac gaaccgacca
     9121 acaacttgga catcaatacc atccgctggc tggaaggcgt gttgaaccaa tacgactcca
     9181 ccatgatcat catctcgcac gaccgccact tcttgaacga agtttgcacc cacatggcgg
     9241 atttggacta caacaccatc actatttatc cgggcaacta cgacgactat atgctcgctt
     9301 ccgcccaatc gcgtgagcgc gccctgaaag acaatgccaa agccaaagag aaactgcaag
     9361 agctgcaaga attcgttgcc cgtttttccg ccaataaatc caaagcccgt caggcaacca
     9421 gccgtctgaa acaggccgac aaaatcaaat cggaaatggt cgaagtcaaa ccttctaccc
     9481 gtcaaaaccc gtatatccgt tttgaagccg atgaaaaagc caagctgcac cgtcaggctg
     9541 tggaagttga aaaactggcc aaacgctttg aaacccagtt gtttagaaac ctcaacttca
     9601 tccttgaagc aggccaacgc ctcgccatca tcggcccgaa cggcgcaggt aaatctacct
     9661 tgctgaaact cttggccggc gcgtacaacc ccgaatattc agacggcctg ttgccggacg
     9721 aaggcagcat caaatgggcg gaaaaagcca gtgtcggcta ctatccgcaa gaccatgaaa
     9781 acgactttga cgtcgatatg gacttgagcg aatggatgcg ccaatggggt cgggaaggcg
     9841 acgacgaaca agtcatccga ggcactttgg ggcgtttgct tttcggcagc aacgacgtgg
     9901 taaaaaaagt gaaagttctc tccggcggcg aaaaaggccg tatgctttac ggcaaactgt
     9961 tgctgttgaa acccaatgtc ttggtcatgg acgaaccgac caaccatatg gacatggaaa
    10021 gcatcgaatc gctgaacatg gcactggaca aatacaacgg cacgctgatt ttcgtctccc
    10081 acgaccgtca gttcgtttcc tccctggcaa cccaaatcat cgaactggac ggcaaaggcg
    10141 gatatgaaca ctacttgggc gattacgaaa gctatctcga gaaaaaaggc gtagcataag
    10201 cgcacccctc cggttgaaac aatgccgtct gaagccgctt cagacggcat tgttgataac
    10261 tttaaaatag gaagcatatg cagacttacc tcgtcggcgg cgccgtccgc gattatcttt
    10321 tgggcctgcc cgtcaaagac cgcgattggg tggtcgtcgg cgcagacgcg caaaccatgt
    10381 tggcgcaagg tttccagccg gtcggcaaag attttcctgt attcctccat cccaaaacgc
    10441 acgaagaata cgccctagcc cgcaccgaac gcaaaaccgc caagggttac gccggtttca
    10501 gtttccacgc cgacaaagac gttacgctgg aacaggactt gatgcgccgc gacctgacca
    10561 tcaacgcgat ggcgcaagat gcggacggca agattatcga ccctttcggc gggcaacggg
    10621 atttggcggc aggcattttg cgccacgtct cacccgcctt cgccgaagat cccgtccgca
    10681 tccttcgtgc cgcccgcttt gccgcgcgtt acggctttga aatcgccgaa gaaaccataa
    10741 agctgatgcg gcagatggtg gaaaacggcg aagcggacgc attggttgcc gaacgcgtct
    10801 ggcaggagtt ggcgaaaggt ttgatggaaa aaaatccgcg caaaatgatt gaaatgttgc
    10861 gcgaatgtgg cgcactccaa gtcttgctgc ccgaagtcga cgccctcttc ggcgtgccgc
    10921 aacgcgccga ctatcatccc gaaatcgaca gcggcatcca taccctgatg acgctgcaac
    10981 gcgccgccga tatgggtttg agcctgcccg aacgctatgc cgccctgctg cacgacttgg
    11041 gcaaagccaa aacaccgccc gacatcctgc cgcgccacca cggacacgac atcaacggcg
    11101 tagaacccgt gcgcgaagtc aatcaacggc tgcgcgcgcc gaggcagtgt gccgagcttg
    11161 ccgaattggt ttgccgttgg cacattattt tccaccaagt cggacagctt aaaagccaaa
    11221 ccattctgaa cgtattgaaa aaaaccgatg ccttccgccg tcccgagcgt tttcagacgg
    11281 cattgaacgt ctgcattgcc gacacgcaag gccgtctgaa ccgagaacac acgccctacc
    11341 cccagcgcgc gcactggctc gccctgctcg aagccgccaa tcaggcggat tcgggcaaaa
    11401 tcgccgccga atgccgcgcg cagggaaaag cgcactttat cgccgaacaa atcgaccggg
    11461 cgcggctggc gcaaatcgcc ccgctgcaaa aagcgtttcg gggcgcgtag gaaaaagaaa
    11521 acgccaaacg cggcatcttt tgaggttcgc ccgcattgcc gaaacagcgt cggagaaagg
    11581 gatggatgac atcggtttat tcggctgcct tttaatattc ccttgatgca ggcctgatta
    11641 gtcggacaaa aatacctttt gttcaacgga accgatttct atccggacgg aaacagccgg
    11701 ctttcccctt gcaaacggat ggaatcaagc gggtattcaa acacagccgt ttatttttat
    11761 tccgccgcca agttatggga tagggtttgc gtgtttcggg cagacaagcc gcgcaacctt
    11821 agacgcagtc cgaagttttg aaggcggcat ccgggccggg ttttgaaaca gccgccgcca
    11881 tatccgctat aatccatcct tcagccattc cgcccccgac ataaaatcat gaccctgaaa
    11941 accgatttat tgcctaaaat caacaacgaa gattatcaac gcctcatcct caaacatagc
    12001 gcggaattca gcggcggaga aacccgcctg ttgaacgaaa tcctcgaaaa attcaatttc
    12061 gacgttgttc aggcgcaggc attggcgcag gcggtaatgc agcaagttcg cttcgatccc
    12121 aacgcctacc acatcgacag cgacgacgaa gacaccaccg gcatctgccc ccactgcatc
    12181 aacccgccta tgccgcccct gcgcgactat ctcgtttggc gcgaaacacg cggataaaac
    12241 gcttttgacc gttatctttt caatgccgtc tgaaacgccg ccgaccgttc ggacggcata
    12301 cccgacaaag ggaacactat gctgcaaacc gacaacctga ccgccgcgca accgcaacgc
    12361 atcgttgccg cccaaaccgc ctccgcacag gaagaactgc tcgaacgcgc cctccgcccc
    12421 aaaacgctgg acgactacat cgggcagcat aaagccaaag agcagctcgc catcttcatc
    12481 caagccgcca aaaaacgcgg cgaagcactc gaccacgtcc tgcttttcgg cccgcccgga
    12541 ctgggcaaaa ccacccttgc ccacatcatc gccaaagagt tgggcgtaaa tttgcgccaa
    12601 accagcggcc ccgtcctcga acgcgccggc gacctcgccg cccttttgac caaccttgat
    12661 ccgcacgacg tattgtttat cgacgaaatc caccggctaa gccccgtcgt cgaggaaatc
    12721 ctctatcccg cgctcgaaga ctaccggctc gacattatga taggcgaagg acccgccgca
    12781 cgttccgtca aaatcgacct gccgcccttc accctcgtcg gcgcgaccac ccgcgccggt
    12841 atgctgacca acccgttgcg cgaccgcttc ggcatcgtct cccgcctcga gttttacgaa
    12901 aaccgcgacc ttaccaccat cgtcagccgt tcggcacaac tgttgcagct cgatatgggc
    12961 gaagaaggcg cgatggaagt cgccaaacgc agccgcggca cgccccgcat cgccaaccgc
    13021 ctgttgcgcc gcgtgcgcga tttcgccgac gtgaaaaaca acggcgtaat cgatgccgcc
    13081 gtcgccgatg ccgctttaag tatgctggac gtggacgcgc aggggctgga cgtgatggac
    13141 aggaaattcc tcgaagccgt tttgcacaaa ttcggcggcg gccccgtcgg tttggacaac
    13201 gttgccgccg ccatcggcga atctacggac accatcgaag acgttatcga accctacctc
    13261 atccaacaag gctttttgca acgcaccccg cgcggcagga tggcgaccga acgcgcctac
    13321 ctgcatttcg ggctgcccgt cgaaaaataa accatgccgt ctgaaaccga accgggtttc
    13381 atacggcatt gttatcagac gtttgcaccg gcaccggttt catcacttaa cagaaacctt
    13441 ttgtccggca ggacagattc tccccgatga aaataactgt ttccctggca aacggttgaa
    13501 acccgtctgg ctgccggttg aaaaataacg caatgctgtc tgaaacagag ctaattttca
    13561 gacggcattt ctatttcaat cattggctca aggttcagcc tgccgctttt tccagttccg
    13621 ccctcatcgc agcgattacc gccttgtaat ccggtttgcc gaaaatcgcc gaaccggcaa
    13681 caaaggtatc cgcaccggct cgggcaacgg cggcaatatt gtcagttttg atgccgccgt
    13741 ccacttcaat ggcaatacgt ctgccgcttt tcccttcata gcggtcaagc atggcgcgaa
    13801 cctgtctgat tttttccaaa gtgtgcggaa tgaagctctg accgccgaac ccggggttga
    13861 ccgacatcag caaaaccata tccagcctgt ccaacacgtt ttccaacaca tatacgggcg
    13921 ttgccggatt caacaccagc cccgcctgac agcccatgtc ccgaatcagg ctcaagctgc
    13981 ggtcgatatg gcggctcgct tcgggatgga aagtgataat cgatgctcct gctttggcaa
    14041 acgattgaat taaatcgtca acgggttcga ccatcagatg cacatcgatc ggcacgcttg
    14101 cataaggctt caacgccgcg cagaccatag gtccgaaggt caggttcggc acataatggt
    14161 tgtccatcac gtcaaaatgg atcagatccg cacccgccgc aatgacattt gccacctctt
    14221 ctccgaggcg ggcaaagtct gccgacaaaa tactgggtgc gatacggtag gcggtcatat
    14281 tttttccttc aatgccttta tgggcggact caacgcggga aagcctcgaa acagcattat
    14341 taacaaaagt taactgccat aataccatct ttcacacggc aatcaagtat attgtccgaa
    14401 atatccggat taaaggaaaa gccgtgccgc tgcctgctcc ctgccgtttt gccaaacctg
    14461 ccgcctcttt tttaagtatg gctttgcttt cctgccagct ttcccacgcc gccacggctt
    14521 atatcccccc gaacgatttt caaccgaact gcgacatacg ccggctcggg ctgacacagg
    14581 gtcagcacaa tgagctgcgt aaaatccgcg ccgccttcaa aatggcgggc gacagggcgc
    14641 gtttgaaggt tatgcattcc gaacacagcc gccgccgctc tgtcgtcgaa atcatttctt
    14701 cggatgtttt taatcggaac gaggcgcgcg attatgtcga aagccgctac cactccagca
    14761 tggattttgc ggtggacgaa ttggaaatcc aacaccgctt cttccatatt ctcacaccgc
    14821 aacagcagca aatgtggctt tcttcctgcc tcaaataatc cccgaaacac tcacggcgca
    14881 cgttgctccg gcagcctgcc cgcccagtcg caggcaaact gccaagcgga acgtcccgag
    14941 cggttgcccc gcatctgcgc ccactgcaac gccgccgtcc gggcggcttc gtcatatggc
    15001 acatcaaaat cttccaacca gcttcgcact gccgccagat aatcgttttg atcgaacgga
    15061 taaaaactga gccacaatcc gaatcggtcg gacaaagata ctttttcttc cacggcctct
    15121 ttttgatgga tttctccgcg cacgcctgtc gtacctgcat tttcatccaa atattcgggc
    15181 atcagatgcc gtctgttgga agtcgcgtac accatcacgt tggcgcaacg ttgggacaga
    15241 ccgccatcca atgccgtttt caatgcctta taggtttcgt cgccgctttc aaatgacaaa
    15301 tcgtcgcaaa acacaatgaa tttttccggg cgttccttca aaagtgtcaa cagatacggc
    15361 aggccgatca aatcgctttt atcgacttcg atcaggcgca atcccttatc cgcatattcg
    15421 tgcagcaggg ctttgaccag cgaggatttg cctgttccgc gcgcgccgct catcaatacg
    15481 ttattcgcag gtctgccggc aataaattgt tcggtattac gcaccagcaa ttcggtctgc
    15541 ctgccgactc cggcaagccg cgacaaaggg aaaacgtgcg gatcgggcaa gtgttccaaa
    15601 aaaccttttt tgcccgcact ctgccagcgg aaggcaagcg cgttccaatc cgtatgcccg
    15661 ggttcgggcg gaagcacggc atccaaacgc cgcaaaacgg cataggcttt atcgagaaat
    15721 tcgttcaatt ccatctctac ctcactttgc atatctttgc gccatcagcc gttcgacggt
    15781 gtcgacgatt gcctgcgtat tcggatcgat ttcgatgttg atcctgtctc cggcctttct
    15841 gctgccgaac agcgtccgtt ccaaagtttc gggaatcaga tggacattga aacggctgtc
    15901 ttcgactttg cctatggtca ggctgcaacc gtccaagccg acgaaccctt tggtcaggat
    15961 atagggtttg agttcgtgcg ggagcgcaaa ccaaaccgtg cggttgaacc cgtcccgttc
    16021 gatttcgaca atgggcacgg ttgccataat gtgtccgctc atgacgtgtc cgccgatttc
    16081 gtcgccgaaa cgtgccgccc gttcgatgtt gacgcaatcg ccttccttca gcagccccaa
    16141 attggttttt gccaaagttt ctgccattaa atcgaagctg acgcggtttc cttcgatttc
    16201 agtaatcgtc aggcagcagc cgttattggc gaccgatgcg ccgtgttgca ggttttctgc
    16261 cgcctcttgc ggaagctcga cgacataagt ctgaaacgcc tccgacgggc ggtggattgc
    16321 cgtcagtttt cccaatcctt gaacaatgcc tgtaaacata atcctgtttc cctgtgtcgg
    16381 taaaaatggt gcaaattgta gcatttcccc gcgaaaaatg ccgtctgaaa ctccttcaga
    16441 cggcattgtg cctccggttc gggcaaaaac cgcccggtac gccttgacct ttcctttccg
    16501 cgccggtcgg cggtcttgca cttatccctc ctgcaaatcg gtttgcgtgt tcaagtcggc
    16561 aaaatgcccg tcaaactcga atctgaccgg ccgcgccctt tgctgctgca accagcttct
    16621 taatgttttc atacccgaaa acagataagg aatcgcgctt tgcagaattt gcggacggat
    16681 atacataatg ttgtagtgca tcgttaccgg cgtttccaca taaaacgcat tgcacaacgg
    16741 tgtgcgtttg gagacgcttt caaagcgcgc gaccaaatcg tccggcagat acggcatatc
    16801 gcaaggtaca atcagcagcc aatcggccgc cgccaactgc aaatcgttgg ctgcggtaca
    16861 caatgccgaa agcgggccga aatgctgcca ctgccgggca tccggaaaaa catgcgggct
    16921 gcggcgggcg tattcttcca aattccggtt ggcactgatg acgatatggc tgacctgcgg
    16981 cctgatttta ccgataacgc ggtcaatcag cggtctgcct tccaacagtg ccagcccttt
    17041 gtcttccccg cccatacggt cggccaggcc tcctgccaaa atcaatgcga acgtttttaa
    17101 tgcgggcggg cttttgggaa agtttgaggt tttcatagtt tcagtctgcc gtttccgata
    17161 ttttagaagc gcgtttgaaa acggcgtcgt ataaattcat gttatttgaa ttttatcata
    17221 acgtgattta acactgaaag cattgaggct taagttttcc ttaacctgtc ctttctgagc
    17281 agttgtttct aattccaaag aatgatattg tttgcaccgt ttaaaacgac tttcctccga
    17341 atatgatact gccaacccga ttttcagacg gcatccccct ttccctgcgc ctgaaactcc
    17401 tgaccggctt gtgggtcggt ttggcggcac tgtctgtcgt tttaactctg ctgctttctt
    17461 tccgcctgga aaatgccgcc tcggtcatcg aagaggcggg caatctgaag atgcaggcat
    17521 accggcttgc ctatatggcg ggcgaaggct cgccccgcgc acaaatcgac aatcagattg
    17581 ccgaatttga aaaaagttta aaacgcattt cccaaagcga tgccatccat ccgctgattc
    17641 cctccgacaa tccccttgcc tacgatttga tacagtccat gctgattatc gactggcagg
    17701 ccaacatcct ccccccgttg caggcatacc gccgccccac acaaatcgag ctttaccgct
    17761 ttgccggcaa tatcgaactg tttttgcagg cgttggaaaa tgccggcgaa aaaaacactt
    17821 ggtggctcag gcggtttcaa tgggtcatta tgctgatgac gctggtgtcg tccgtgctga
    17881 tgcttttttg gcaccagatt tgggttatcc gtccgctgca ggcgttaagg gaaggtgcgg
    17941 aacgcatcgg tcagcggcat ttcgacatcc ctgttcccga agacggtacg cccgaattca
    18001 aacaggtcgg gcggtgtttc aaccaaatgg cgctcaggct gaagacttta tacgatgatt
    18061 tggaaggaca ggttgccgaa caaacccaca accttgaaaa gcaaaaccgc aacctgaccc
    18121 tgctgtaccg gaccacgcgc gacctgcatc aatcctatac gccgcgacag gcggcggaag
    18181 aatttttgaa ccacatcctg cccgccgtcg gcgcgcaatc cggcaacatc tgcctggaaa
    18241 acggttccga cacagacatt tccgtccata cggccgaaca cggcaaaaag ccccctttgg
    18301 aaaaatacca tgacgaaacc ttccccatcg aatatcaaaa cgagaaactg ggcatgcttt
    18361 cgctcggctt ttcagacggc acttccctca ccggagacga ccgcacgctg cttcaaaccc
    18421 tgattcggca attgggcgta tcgctcgccg gggcgaaaca ggaggaagaa aaacgtctgc
    18481 ttgccgtatt gcaggaacgc agcctgattg cacaggggct gcacgacagc atcgcgcagg
    18541 cattaacctt cctgaacctg caggtacaga tgctggaaac cgcctttgcc gaaaacaaac
    18601 gggaggaagc cgcagaaaac atcggcttta tcaaaacagg cgtgcaggaa tgttatgaag
    18661 atgtccgcga actgctgctc aatttccgta ccaaaatcag caataaagaa tttcccgaag
    18721 ctgttgccga cctgttcgcg cgcttcacgc aacaaaccgg gataacggtc gaaaccgttt
    18781 gggaaaacgg ttcgttcctg cctacacagg acgagcagct ccaaatgatt ttcatcctgc
    18841 aagaaagcct gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt
    18901 ccgaatacgg cggacgcttt accatgacca tccaagacaa cggacaaggt ttcgacacgg
    18961 agaaaatagg agaacctacg ggcagccatg tcggactgca catcatgcag gagcgtgcca
    19021 aacgcatccg tgccgtttta gaaatccgtt cccaagcaca acagggaacc accgtctcat
    19081 tgacgggcgc acccaaagaa agcctaccat gaccatcaaa atcatcctga tagacgacca
    19141 caccctcttc cgcagcggca tcaaagccct tttgtcgcgc caacacggtt ttgaagtcat
    19201 cggcgaagcc gcagacggac tctcgggcat caaaatgatc agtcggctgc aacccgatgt
    19261 cgtcctgctc gatttggata tgccaggcat gaacgggcgc gaggccttgt cccaaatcat
    19321 cagcatcaat ccgcagcagg cagtcattat gctgaccgtt tccgaagaca gcgacgacct
    19381 taccgaatgt atgcgcatcg gcgcgcgcgg ctacctgctg aaaaacatca acgccgattt
    19441 tcttctcgaa agcatacgca aagccgccga aggcgataat gtattctcgc ccgagatgac
    19501 cgccaaactc gtcaaaagcc tgatttcccc ccaacctgcc caaaggacgc aggcactctc
    19561 ctcacttacc cctcgtgaac tggaaatctt gggctatctc gccgcaggac acagcaacaa
    19621 aatcatcgcc cgccacctcg atcttgccga atccaccgtc aaagtccacg ttcaaaacct
    19681 gctccgcaaa ctcaacctca gcagccgggt gcaggccgcc gtttacgcca tccggcacaa
    19741 cgtcccccaa cctgtgccgg aatagacgtt cagacggcat cgggtcagga aaaaagcaat
    19801 gcgccccttt gacggggtgc aatatataag ggacggcaca ccggatgccg tccctttaag
    19861 tacgccatta ccgccgtaac ccggacggaa tggcttaggc agtttatttc caagaatatt
    19921 tcacacccac ggtgccttgg tagcctttat gtctgttgct tccgcatcct gccgataatt
    19981 gccttgcctg tcccgagaaa gggtttgatt ttcgccacgg cgttggtgga gcgtatccag
    20041 catcgcatag cctgattcca agttttgacg tggaacgagt gcgtaggccg gtgcgcttgg
    20101 cgtgatgatg gttttatctt gattcggcgt ggttaaagtc cagaagtatt ctgtcgcgcc
    20161 cgcctcatct ttgtgtgaaa ccaattgcaa ttcccccgcc cctgtggttt tggcagttcc
    20221 gctaaaagta tcgcgcattg tgtaggtatg tttagcgtct tcgatattta atttaccggt
    20281 ttccgccccc ttgtcttgtc ccaaaacctt aacaacggcg gcactgcgtt tgtagcggtc
    20341 ggaaagttcg ccgatcgagc cgtcgataat attttctttg ccatctttac ccactgaaat
    20401 gaccgttgtt tttcctgaag catcgcctgt gatttccagt aaatcagatt gagaatttcc
    20461 tccttgatca tcacccggcg agttccattc tgtattgact tttaacacac cgtctttacc
    20521 gacataattt ccttcaatgg tgagtttgtc tgcataggat tgattggcaa gcgtaatgat
    20581 gccgccgttg gtgaatgttc ctttttcaaa tacagaagtt gtacccgata aagaaatacc
    20641 tgtattagat agggttagcg tgccgcctga taataatgta gtcccttcgg gggttgttaa
    20701 tgatgtgtct ttttttaggt gaaaagtagc ttttgttagt tttatggttc caatatcttc
    20761 tgatttgaat tccattgcgc catttgtaat ttttagcaca gcattggtaa tagggagggg
    20821 gaagcgcatt gtaaatgatt tttctgttcc atctggattg attaatgtac cagatgcgcc
    20881 aaagatctct ccttcaggga tagaaacagt tccatcagaa attgatggtt tatttgcgct
    20941 tttatcactg tgtttatgaa ttaatgtaat tggggctgta ctagattatc cctaaattcc
    21001 acaccgatcc cgcaggattt ttagctgccg ggacggtgtg ccg
//
LOCUS       DAWTHD010000022        20911 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWTHD010000022 DAWTHD010000000
VERSION     DAWTHD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20911
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.022"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(2..466)
                     /locus_tag="WM704_000669"
     CDS             complement(2..466)
                     /locus_tag="WM704_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340142.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGAGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDARLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="WM704_000670"
     CDS             complement(543..3380)
                     /locus_tag="WM704_000670"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFA9340143.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGITSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="WM704_000671"
     CDS             complement(3485..4744)
                     /locus_tag="WM704_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFA9340144.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="WM704_000672"
     CDS             complement(4823..6214)
                     /locus_tag="WM704_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9340145.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGTGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(6476..6580)
                     /locus_tag="WM704_000673"
                     /pseudo
     CDS             complement(6476..6580)
                     /locus_tag="WM704_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="WM704_000674"
     CDS             complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="WM704_000674"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFA9340146.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7496..8176)
                     /locus_tag="WM704_000675"
     CDS             complement(7496..8176)
                     /locus_tag="WM704_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFA9340147.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="WM704_000676"
     CDS             complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="WM704_000676"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFA9340148.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="WM704_000677"
     CDS             complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="WM704_000677"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFA9340149.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTIVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9944..10444)
                     /locus_tag="WM704_000678"
     CDS             complement(9944..10444)
                     /locus_tag="WM704_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFA9340150.1"
                     /translation="MTRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="WM704_000679"
     CDS             complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="WM704_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFA9340151.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="WM704_000680"
     CDS             complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="WM704_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFA9340152.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14320..15555)
                     /locus_tag="WM704_000681"
     CDS             complement(14320..15555)
                     /locus_tag="WM704_000681"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFA9340153.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15560..16357)
                     /locus_tag="WM704_000682"
     CDS             complement(15560..16357)
                     /locus_tag="WM704_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFA9340154.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16360..17106)
                     /locus_tag="WM704_000683"
     CDS             complement(16360..17106)
                     /locus_tag="WM704_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFA9340155.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17162..17719)
                     /gene="frr"
                     /locus_tag="WM704_000684"
     CDS             complement(17162..17719)
                     /gene="frr"
                     /locus_tag="WM704_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFA9340156.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17861..18853)
                     /locus_tag="WM704_000685"
     CDS             complement(17861..18853)
                     /locus_tag="WM704_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9340157.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18858..19373)
                     /locus_tag="WM704_000686"
     CDS             complement(18858..19373)
                     /locus_tag="WM704_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFA9340158.1"
                     /translation="MTACLQKPAKMRKNGKRKTLSIIVGVVDKKKNLKHLAMVYGIDY
                     CADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNRIDPLNITYLRVRGMWGIENPW
                     FVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLAIQDSKLAISDIKIKNPNNPAR
                     LRNAKLITYHL"
     gene            complement(19291..19695)
                     /locus_tag="WM704_000687"
     CDS             complement(19291..19695)
                     /locus_tag="WM704_000687"
                     /inference="COORDINATES: protein motif:HMM:NF021068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFA9340159.1"
                     /translation="MNIIDAIINLANNPVVGVESHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(19804..>20911)
                     /locus_tag="WM704_000688"
     CDS             complement(19804..>20911)
                     /locus_tag="WM704_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFA9340160.1"
                     /translation="RLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARLLHLQHGE
                     WIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTI
                     GTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIGAAIAIAA
                     AKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQINARMVKS
                     RSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRH
                     FTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEI
                     SALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
BASE COUNT         4238 a         5305 c         5991 g         5377 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtgcat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct gcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccatgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtt tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc tccgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaaacgggc gagcatgatg ctgtcggcgg ttttggcatc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctggcggct ggcgcagccg
     1201 gtttggagct gcactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctggtga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcaccgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgataccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggtcacggt ttcggcgcgt
     2941 acgccgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc caagaatttt
     3061 tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggccgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcggt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtaaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaacacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgataccgaa gctggtttgc acagcctgtg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcgaatgtg ccgaacagcg caaatgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
     5821 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccagaat accgcgcccg ggccgccggt taccactgca gtcgccacgc
     5941 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg
     6001 aaatatcgcc ttcgtggcct gtgccgcctt catacttctt tgacggcaca aacgcctgtt
     6061 tcagcgcata gcccaacata gtgaactgca agcctttcag cataacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccaaac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc acttttgtag tgtccggtaa tttcagcaca gaatattcaa taagacaata
     6301 tgttcttttg aaaaatactt ttggtttttt caccgaagac aggccggttc aagctgcgga
     6361 aattgtttgc aattatttaa aagcagaggc ggaggtcaca atgaaatgtc cgaatgggga
     6421 tgtggcgggc ggcagaaatc atcaatgctg ccgactgcca tacttctgaa atctatagtg
     6481 gtttaaattt aaaccggtac agcgttgcct cgccttgccg tacttcttgt actgtctgcg
     6541 gcttcgtcgc cttgtcctga tttaaattta aaccactata caaaatgatg catcgatcaa
     6601 acaacatacc gctttaaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
     6661 cattgaaagg gtgcggtcag cggatgatgc cgcgcgtcga ttgtgcgaag aagtcttgaa
     6721 atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtccgccttg gcttcttcaa
     6781 acggaatgcc gcgatggtag agggttttgt atacgtcttt gacggcggaa atctgttctg
     6841 cggtaaaacc gttgcggcgc ataccttcgc tgtttaggcc ggccggttcg gcgcggtagc
     6901 ccgatgccat aaagtagggc ggcacgtctt tgtgtacgcc tgcggcgaag gcggtcatgg
     6961 cgtagtcgcc gatgcggcag aattggaaga ccagcgtgta gccgcccaaa acgacgtagt
     7021 cgccgacggt aacgtgtccg gcaagcgagg cgttgttggc gaaaatggtg tggttgccga
     7081 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
     7141 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
     7201 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc ggaatttcgc
     7261 cgaggctggc aaattgaaaa atgcggttgt tttcgccgat ggtggtgtgg ccgttgatga
     7321 cggcgtgcgg accgatttct gtattcgcac cgattcggac gttggggccg ataacggtgt
     7381 acgcgccgac tttgacgccg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
     7441 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaatcaca
     7501 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
     7561 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtaaata tttctagcgt
     7621 agccggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc aaacgattac
     7681 ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
     7741 gtgtagattt gggctttttt ggaaaaactt tcatcaatac ttttcctgca gcatcaaatt
     7801 gagcatcttt ccctcccgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
     7861 attggttgtg gtcaaacttt cctccgctat tattttgtat tttagataat gcaaccttga
     7921 ttaattcagc agattctttc gtaccatttc ttaaaaagca accttgatat ttcaatgcag
     7981 gattcgtttg tggttgtatg ctatcatata accggtttct gtctttaaac tcctgccgaa
     8041 agcaaatatc aattttgttt cttaaaacga tctcaaatat agaaacaaca tgaaaaagtt
     8101 ctcttgataa agataaattc aataaataca attcagctgc tttgtctttc ctaacggcat
     8161 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taattcacgc
     8221 taacccttga ttctttattt ttaaattgac gtttcaaact atatttgata tagttagata
     8281 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
     8341 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
     8401 gcacataatc acggcttcga cggcgacttg tccgtccact ttggcaacgg cgttgaattt
     8461 gccgatgccg cgccggctgg tcaggagttc gacttcaaag acgagttggt cgccggggat
     8521 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaaaaatt cgttttcctt
     8581 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcacgcc tgcgccatcg cttcgatgat
     8641 gagtacgccg ggcataacgg gcaggtcggg gaaatggcct tggaattggg gttcgtttat
     8701 ggttacgttt ttgatggcgg tcagggtttt catcggctcg aaggcggtaa tgcggtcgag
     8761 ctggagaaac gggtagcggt gggggatgag tttttggatg tctttggctt cgatggggag
     8821 ttgtacgtcc atgtctgtcg tattccttga ataaagtcgg tttggattta ttgcgtgtct
     8881 ttgctgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
     8941 tggatgtaaa cggcgttgcg cgcccattct ttatgggtgg acatcgggaa gatgccggcg
     9001 atgtgtttgc cgctttcggt aatgctgtgg gtaacggacg tgccgccgcc gatggtggtt
     9061 ttgtcggcga tttcgatgtg tccgaccgtg ccgactccgc cgccgatgat gcagtagctg
     9121 cctatggtta cactgccgga gatgcccgtt ttggcggcga tgacggtgtg cgaaccgatt
     9181 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacaatggtg
     9241 tcgctcatcg cgccccggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
     9301 gttaccgcgc cggtttgcgg gattttgaac cacgaatcgc cggcgaaggc gagtccgaaa
     9361 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgt gtctgccgag tgtgcagccg
     9421 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
     9481 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgttcgc gccgatgtag
     9541 gcgttcgcgc cgatttcaca gcttgcggga acggtcgcgc ccggttcgac gacggcggtc
     9601 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgcgcgac tttggcgaaa
     9661 tagagatagg gatcgtcggc gacaatcagg ttgcgccctt caaatccgtc tgctgctttg
     9721 gcggaaacga tgatcgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
     9781 gcgaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
     9841 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgcaggtg
     9901 gccggaatca tggttttcct gtttggtatg ccgtctgaaa gggttagcgg gcgttcattt
     9961 ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg tcctgcaaaa
    10021 tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg ttttgctgga
    10081 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    10141 tgcggaacgc ttcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtcct
    10201 taagtttgcc gccggcgagc tgcctttcca aatccaagcc ttcgcgttgc agtttttgca
    10261 attcgtcctg acgggcggaa aattcgccgt ccagcgtttt ttggatgttg cgcgcctgct
    10321 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    10381 gcgtgcctgt ggtgcagcat aaaccgatca gggtggcggc aaacgcgcgg gtcaaacggg
    10441 tcatggtaaa actccttcgg atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    10501 tttgcgggat tagaacgtcg tgccgagctg gaattggaag cgttggattt cgtcttccgg
    10561 ttttttcttc agcgggtagg cgtagctgaa tttcatcggg cccaaaggcg agagccaggt
    10621 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gcgcgttttc
    10681 cgagtaaacc gatttgttgt taccgttttc ggcggcggta taggttctgc cgtcccacac
    10741 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgt gcgtctttcg caccgggcat
    10801 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    10861 gccgtattcg tcatacactt tcgggccgag cgtgccgctt tcgtagccgc gcaccgaacc
    10921 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    10981 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    11041 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    11101 atttacgccg gtcaggtagc cgcgcgtcgg ccataaggcg ctgtcggtct tgttgcgccc
    11161 ccagccgaca gtgcctttgt acagcaggcc tttgaagctg ccgtctgcgc cgtcggtttt
    11221 gccgtattgt ttgataaagt cggcatagcg tttgggtgct ttgttgtagg tgttgacggt
    11281 caggtgttcc gccgccagcc cgaaattgac gcggtcgtat tcggtaacgg ggatacccat
    11341 ccttacgccg ccgccggcgg tggtggtttt atattgtttg acgctggtcg atgctttgcg
    11401 cgggtcgaag gcttttccgt aaatatcgta gcccaggctg accccgtctg ccgtgaagta
    11461 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgcgagg cgcgcagggc
    11521 ggccgacttg cccgtaccga acaggttgtc ctgcgatacg ccggcggaca tgaccaagcc
    11581 ggtatcctga acccagcccg cgctcaagtc gagcgagccg gtggagcgtt cggtcaggct
    11641 catgttcaaa tcgactttgt cgggcgtgcc ggcaagcggg acggcatcaa actgtacgtt
    11701 gtcgaagtag cccaaaagct cgacgcgctc tttggaacgt tgcagcttgg aggtgtcgta
    11761 aggcgcggat tccatttggc gcaattcgcg gcggacgact tcgtcgcggg ttttgttgtt
    11821 gccggtgatg tggatttcgt tgacgtagat ttttctgccc ggttcgatgt gcaggacgaa
    11881 atcgacggtt ttggttccgg cgttcggcag cggctgtacg ctgatttcgc tgtatgcgta
    11941 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    12001 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggccttgg ggacttcgtt
    12061 ggtgtcgcct tcaatcgaca ctttgcccca gcggaaacgt ccgccttcgt ggacggtgat
    12121 tttgatggtc tgcctggttt tgtcttcgtt ggtttggatg tcggtatcga ggatacggaa
    12181 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcgaattt
    12241 ctggcggtcg aaccggtcgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    12301 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    12361 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    12421 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    12481 caggccggcg actgcctggt tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    12541 gaacgattcg aggtttttct tgatggcgtc gttttgcagc attttggcgc cggtgatgtt
    12601 gagcgagccg atggtggggc gttcgataac ggtcagcaga agctgcccgt ccgcagtttc
    12661 gactcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    12721 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tgctcggctc
    12781 ggtacgctgc aagccttcga cacggatgtc ttggatggtg aagtcggcaa atgccaaagg
    12841 cgatatgccc aacatcatca gtgcggaggc aatctgtttc agtttcatcg taagttcctt
    12901 gtggtgcgga atgcggtttc agactgcatt ccgaaacgta aaatctaacc gatcagccgg
    12961 gtaacgtcgt tgaagaaggc ggccgccatc atcagcatca tcagggcgag cccgaagcgc
    13021 aaaccgatgt tttggacacg ttcgcccaaa ggtttgccgc gtatccattc gacagtataa
    13081 aacacgaggt gcccgccgtc caaaacgggg acgggcagca ggttcagcac gccgaggctg
    13141 atgctgacca acgctaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    13201 gcaatgtcgg caatggtcag cggcccggaa atatggctga cagaggcgtt gccgctgatt
    13261 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    13321 ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    13381 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcgggctg ttcgacagta
    13441 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggtcag ggtgattttt
    13501 ttgcccgggc tttggcgggt caggtttgcc cattcctgcc atgaggcgat gggtttgccg
    13561 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    13621 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    13681 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    13741 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    13801 gtttgcgcgc tgctccagtc ttggacggaa acgccgttga cggattgtat tttgtcgccg
    13861 ctttggaagc cggtgcgggc ggcaatggtg tcgggttcga ctgtgccgac atagggccgc
    13921 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    13981 gtcagcggac cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    14041 tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt gacgtagccg
    14101 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    14161 ttgccgaagc cgacggaaaa acgcacaacc ttgacgccgc acaacctggc gacgatgtag
    14221 tgtccgaatt cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtt
    14281 tgcaaatggt tttcctttga taacggtgtt cggacggtat cagcgcagtg tgccgataaa
    14341 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaac agtccttcta tatcgcccat
    14401 gccgtttgaa aagtcttgtg caagacagtg ggcgacggtt ttggcaatgt cggtaaactt
    14461 aatctgtccg tccaaaaagg cggcgacggc ggtttcgttg gcggcgttca atacgcaggg
    14521 cgcggctccg cctgcgttta tggtttcata ggcgaacttc aggcagggga agcggccgaa
    14581 gtcgggcttt tggaaggtca gcgcggacaa tgcgccgaaa tcgagtttgc cgacacccga
    14641 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    14701 cagttgcgcc agcacggagc cgtcgcggta gcgcaccata ctgtgtatca cggattgggg
    14761 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    14821 ttccaagccc ttgtttgcca tagtggctga atcgacggag attttgcgcc ccatacgcca
    14881 attggggtgt ttgaccgcct gctcgggcgt aatgctgtcg aacgtgctta aatcggttgt
    14941 taaaaacggg ccgccggaag cggtcaggat aatcgaatcg atgccgtgtt cgttcagacg
    15001 gtctgtgtaa tcgcgcggca aaacttggaa aatggcgttg tgttcgctgt cgacgggcaa
    15061 cactgccgcg ccgtttgcgc gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    15121 ttctttgttt gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    15181 ccccgccgcc ccgacgatgg cgcacatgac gccgctgact tcgtcggcag acgcaacgtc
    15241 aaccaatgcc tgcgcgccgt gtaaaacctg agtcgccgtg ccgtcgcgtt tcaacagggc
    15301 ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc ggaacgtttg
    15361 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    15421 ttcgggatgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    15481 tatggtcagg acttgtggtg tcataatgag gataacttta taccggatgc cgtctgaagc
    15541 gttttcagac ggcatagaat caatttaaaa ccgacattat cgccgcatag acgctgataa
    15601 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc aggttgctgc
    15661 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa caggtcgccg catacgctga
    15721 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    15781 agccggcact tcgtacggca gtcatgtaca ctgccacgca aaccgcaccg ccgattgcgc
    15841 cttcccagct cttgccgggg ctgattgccg gtgcgatttt gtgtttgccg agcgccttgc
    15901 cgctgaaata cgcgcaaacg tcggcaaccc agaccaaacc catcacggcg agcagcggca
    15961 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgagcaaaa
    16021 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccaca
    16081 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    16141 agccgcccgc ataggcaact acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    16201 cggttttgca caaaccggcc atacgggcat actcccacaa ggcggtcagg gcaatcagcc
    16261 cgcaaaatgc agcccacagc cattgcggcg cgtaaaacag catgcccagc atcagcggca
    16321 gcagccacat agcggttatt acccgttgtt tcagcatatt cagttccttt gctgtccgat
    16381 aggcagttgc tcggaagtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    16441 ggcatctaag gcggtttcgt cgaaatcggg ccacaggatg tcggtgaaat acagttctgc
    16501 atacgccatc tgccagagca ggaaattgct gatgcgcgtt tctccgcccg tgcggatgaa
    16561 caaatccggt tccggcgcgt cgcccagcat caagtgtttg gccagcgtgt cttccgtaat
    16621 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc agcggccgcc
    16681 gtaatccgca gcaatgctca gggtcagacc ggtattattt gccgtcaagg cttccgcctc
    16741 ttcgatacct tgcagaatct gccgattaaa gcgttcgcgg ctgcccaata ttttcaggcg
    16801 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    16861 caggaacgaa acttcgtctt cggggcgacg ccagttttcg gttgaaaagg caaacacggt
    16921 cagatattgc acgcccagtt tggcgcaatg cttcaccata ttttccaacg cgtccaagcc
    16981 gcgtttgtgt cccattatac gcgggagaaa acgttttttc gcccaacggc cgttgccgtc
    17041 cataattacg gcgatgtgcc tcgggatggc ggtgtgttcc aaaatggtct gcgtgctgct
    17101 cttcatatct gcctttcgcg gttcggcgtt caaatgccgt ctgaacgccg cgccgtgaga
    17161 tttaaaccgc catcaggtct tcttctttgg cagccaaaac cttatcggct tcggcaatgt
    17221 acttgtcggt cagtttttga accgcctctt cgccgcgacg tgcctcgtct tcggaaattt
    17281 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatag
    17341 agacgcgtcc ttcttccgcc tcgccgcgca cgactttaat caggtctttg cggcgttcct
    17401 cggtcagcat gggcatcggc acgcggatca aatcgccgac agctgccggg ttcagtccca
    17461 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    17521 cgccgatggt gcgcgcgtcc aaaagggtta cgttggcaac ctggctgacg gggaccatgc
    17581 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    17641 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    17701 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaggcggg cagccgttcg
    17761 aacgcgctcc aagccgttca gacggcataa agaccgttaa ctgcgaatag taccgttatt
    17821 cgggcatgac gacaaggcag atgcctgtag gaagggtgtt tcacctttct agggtttttt
    17881 taattgccgc tgcaatttcg taggcaaggt tgacggggac ggcgttgcca atcattttgt
    17941 atgcgtcgtt gacattttga tagatgaatt tgaagttgtc gggaaagcct tggattcttg
    18001 caacttcgcg taccgtcatc ctccgatata gtgtttcttt gccggcagca aaacggtagt
    18061 cgtttgcccc gtgcttttcc atctttgggg cttgcgggtg cagttggcac tgcctgcctg
    18121 aagcttggac ggtaaaaccc tgttcatccc acgctttaac tcggttccgg ctcataaaaa
    18181 taggggaaaa actgccggta aaatattcgt tgttgttgac tgcgtcgggg ttggtcttgt
    18241 tttgcggggc ggaaggtacg gctgtgtcct gcaaatccca aataacgtct ttcaatgtaa
    18301 tcttgtcttt gtcttcgacc gtcgaacctt ttggaaaaga aaattttatt tccaagtctt
    18361 tacggaaacc gatgtagaag acccttttgc gttcctgtgc tacaccgtag tctttggcgt
    18421 tggccatagt caaggttacg tcgtatccgc atccgtcaaa cattttcagc aggttttgta
    18481 cggctccgtt gtggcggttt gccagcattc cgctgacgtt ttccgctaaa aagaattttg
    18541 gctgtttgct tttcaaaata cggatgtagt cgaaaaacaa ctgtccgcgc gcatcgtcga
    18601 tgccgcgcaa agctcccgct tcagaccaag actggcaggg tgggccgccg ataatcccgt
    18661 cgatttcttc agggaaatct tcttctttaa tcttgcgtat atcgccttct atcaaatggg
    18721 tctttggatg gtttgccttg aaggttgccc aaatggtttt atcgtattcg ttggcggcgg
    18781 ggatttcaaa tcccgctttt tcgaaaccca aatccaaacc gccgcaaccg ctaaacaaac
    18841 taatgatttt cataggatta taagtggtag gtgatgagtt ttgcatttct taatcgcgcc
    18901 gggttgttgg gatttttaat tttaatgtcg gaaatggcca gtttggaatc ctgtatggca
    18961 agcagcttgt ctgtattgtt aaaactgttc catttgtctt cgttgataat ggccatgaaa
    19021 ttgaaacttt tttccatatt ccgttggtaa atatagttga agacaaacca tggattttcg
    19081 attccccaca taccccgtac tctcagataa gttatgttga gcggatcaat gcggttgacc
    19141 ctgcccagct ctttggtttc tgcaaactgt atgccgccga tgttgccgat tccttcctta
    19201 atttgatttt ttatcttcaa atagcattcg gcatcggcgc agtagtcgat tccgtaaacc
    19261 attgccaagt gtttgaggtt tttctttttg tcaacgacgc cgacaattat agataatgtc
    19321 tttctcttcc catttttccg catctttgca ggcttttgta agcatgctgt catcaacaga
    19381 gagctttgat tttgggtggc tgctattcag cgccagtgcg gaatctttac tttcaatttt
    19441 cttaacttca atggcatccc cgtttcgcaa catggcatca ggagggttgg agttgttgcc
    19501 caaatatgag aatacttttg cgtgtcgggc tatgcgttgg gtttcattta ggttgaaact
    19561 gccggaaaac aagtctttta catattcttc caaagcatca cctgcctgat tggcacgatt
    19621 gttgctttga gaatgggatt cgaccccgac aacagggttg ttagccaaat tgatgatggc
    19681 atcgataatg ttcatggttt ttccttgggg ttttgcggca gattatagca aaaccgtcag
    19741 ggtgtttatc ggaccgatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccgg
    19801 ccgtcagccg tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc
    19861 atccagccat ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg
    19921 catattggtg ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc
    19981 ttccagttcg gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat
    20041 gagggcggcg gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt cgagcagccg
    20101 gatttcgctg ccgttgagtt tgggagattg cagcttggcg gcggtggtca ggagcagctc
    20161 ggtggtgttg acgattttgc ggtgggcgtg ctgcatggct tccatcatgg aggggctgat
    20221 gcggctttcg cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat
    20281 ttggcgcatt ttgaccatat tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct
    20341 gatttcggca atcattttgc tgcagtcggc caggttgtcg gcaagcatga aacgccacat
    20401 cagtgtggat ttcagcggca gcagtttggc ggcggcaatg gcgatggcgg cgccgatgag
    20461 gacgttcatc gcgcgcatca ggccgctgtc gagccattcg ctgccgttgt cgccgatgag
    20521 catgcacatc gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca
    20581 gccggccagt gcgcttgccg tgccgatggt caggtagaag aggaggttgc cgtggaaata
    20641 atgctggttc agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg taccgagcat
    20701 acgttccacc gccttggagt aaatcgcgcc ttggaactgg agcatgccga ggacgacgaa
    20761 gacggtcatc cctatccatt cgccgtgttg gaggtggagt agccgggcga gtgcggtggc
    20821 gaacaggacg gctccgccga gccgcacggc atgtatgagg cggcggtggc ggtagcgttc
    20881 gtaggagttg agccagcggc cggaaaggcg t
//
LOCUS       DAWTHD010000023        20082 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWTHD010000023 DAWTHD010000000
VERSION     DAWTHD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20082
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.023"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            282..1754
                     /gene="pyk"
                     /locus_tag="WM704_000689"
     CDS             282..1754
                     /gene="pyk"
                     /locus_tag="WM704_000689"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFA9340161.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1913..2512
                     /locus_tag="WM704_000690"
     CDS             1913..2512
                     /locus_tag="WM704_000690"
                     /inference="COORDINATES: protein motif:HMM:NF015316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HFA9340162.1"
                     /translation="MPFLFVHRPPFYGNRNDPFHTEKTVLLLGTAFAAASVHASGYHF
                     GTQSVNAQSTANAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGL
                     PVQGSKNGKITKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKL
                     GLTSIAVEPVAAWKLNERHSFGAGIIAQHNSAELRKYAD"
     gene            2534..3340
                     /locus_tag="WM704_000691"
     CDS             2534..3340
                     /locus_tag="WM704_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFA9340163.1"
                     /translation="MLQATPSNPTAAAQIKADGHADVKGSDWGVGYQLAWMWDINDRA
                     RVGVNYRSKVSHTLKGDAEWAADGAAAKQQWNDNMLTPLGYTANEKARVKIVTPESLS
                     VHGMYKVSDKADLFGDVTWTRHSRFNKAELFFEKEKNIANGKKSDRTTITPNWRNTYK
                     VGLGGSYQISEPLQLRVGIAFDKPPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHVV
                     DAAYTHIHINDTSYRTAKASGNDVDSKGASCARFKNHADIIDLQYTYKFK"
     gene            complement(3423..3716)
                     /locus_tag="WM704_000692"
     CDS             complement(3423..3716)
                     /locus_tag="WM704_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340164.1"
                     /translation="MKANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(4028..4177)
                     /locus_tag="WM704_000693"
     CDS             complement(4028..4177)
                     /locus_tag="WM704_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340165.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(4195..4392)
                     /locus_tag="WM704_000694"
     CDS             complement(4195..4392)
                     /locus_tag="WM704_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340166.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(4402..5418)
                     /locus_tag="WM704_000695"
     CDS             complement(4402..5418)
                     /locus_tag="WM704_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFA9340167.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMATQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(5517..6731)
                     /gene="gltS"
                     /locus_tag="WM704_000696"
     CDS             complement(5517..6731)
                     /gene="gltS"
                     /locus_tag="WM704_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFA9340168.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(6839..6943)
                     /locus_tag="WM704_000697"
                     /pseudo
     CDS             complement(6839..6943)
                     /locus_tag="WM704_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6984..7265)
                     /locus_tag="WM704_000698"
     CDS             complement(6984..7265)
                     /locus_tag="WM704_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340169.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(7284..8408)
                     /locus_tag="WM704_000699"
     CDS             complement(7284..8408)
                     /locus_tag="WM704_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9340170.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(8471..9478)
                     /locus_tag="WM704_000700"
     CDS             complement(8471..9478)
                     /locus_tag="WM704_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFA9340171.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(9471..10472)
                     /gene="dcm"
                     /locus_tag="WM704_000701"
     CDS             complement(9471..10472)
                     /gene="dcm"
                     /locus_tag="WM704_000701"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFA9340172.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(10503..12776)
                     /locus_tag="WM704_000702"
     CDS             complement(10503..12776)
                     /locus_tag="WM704_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HFA9340173.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            12891..13907
                     /gene="galE"
                     /locus_tag="WM704_000703"
     CDS             12891..13907
                     /gene="galE"
                     /locus_tag="WM704_000703"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFA9340174.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            13908..14948
                     /gene="rfbB"
                     /locus_tag="WM704_000704"
     CDS             13908..14948
                     /gene="rfbB"
                     /locus_tag="WM704_000704"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFA9340175.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            15053..15919
                     /gene="rfbA"
                     /locus_tag="WM704_000705"
     CDS             15053..15919
                     /gene="rfbA"
                     /locus_tag="WM704_000705"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFA9340176.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            16057..16614
                     /gene="rfbC"
                     /locus_tag="WM704_000706"
     CDS             16057..16614
                     /gene="rfbC"
                     /locus_tag="WM704_000706"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFA9340177.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRIVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(16658..18676)
                     /locus_tag="WM704_000707"
     CDS             complement(16658..18676)
                     /locus_tag="WM704_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HFA9340178.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSSF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAALQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(18871..19992)
                     /gene="dnaJ"
                     /locus_tag="WM704_000708"
     CDS             complement(18871..19992)
                     /gene="dnaJ"
                     /locus_tag="WM704_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFA9340179.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         4973 a         5524 c         4893 g         4692 t
ORIGIN      
        1 ggcttagacg actttttctc atagggataa ttctaactta atttgaattt ccctagttat
       61 ctaggacagc cccaaaataa atttagccta aatcataaaa ccgtgtaatt ttgcaccaaa
      121 aaaggcggtt tgcaggtaaa atatcaaacc attaatttaa atcatatttt tacagaatat
      181 tccgccgcgt tcaccaaatg gacatcaaac cggttccaaa tttctgtaat tttgcaacaa
      241 aataccgcaa aacacccgat tgagaccgaa aggactttca tatgaaccag acaagccgcg
      301 atctgacccg catcagccac aacaccaaaa tcgtcgccac ccttgggccg ggcagcaaca
      361 acgtcgaact gttggaagac atgatccgcg tcggcggcct gaacgtcgtc cgcttcaact
      421 tcagccacgg cacgcccgaa ttccatcagg aaaacgcccg catcgtgcgc gaggcggcaa
      481 aacgcgccgg acaggaaatc gccatcattg ccgatctgca gggcccgaaa atccgcgtgg
      541 gcaaaatcgc cggcggcggc atcgaattga acaaaggcga aacattggta ctcgatgccg
      601 cgctcgaagg cgaaggcacg cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg
      661 acgttgccgc aggcgatgtc ttgtggctgg acgacggcct gctgaccctg accgtggaat
      721 ccgtcgaagg cagcaggatt atcacaaggg tggaaaacag ccacatcctg aaaagcaaca
      781 aaggcatcaa caaacgcggc ggcggtttgt ccgcaggcgc gttgaccgaa aaagacttcc
      841 gcgacctgaa aaccgcgatt gccatcggtt gcgactatct cgccatcagc tttgtgaaat
      901 ccgccgaaga cctgcacatc gcgcgcgcca aagtcgaaga ggaaatgaag ggcagcactg
      961 ccgtccgccc cggtttggtt tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg
     1021 aaatcatcct tgccggcgac ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg
     1081 gacacgccgc cgtccccgcc ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc
     1141 gcttcagcat tacggcgacg caaatgatgg aatcgatgat taccaacccc gtgccgaccc
     1201 gcgcggaagt cagcgatgtg gcaaacgcgg tattggacgg caccgatgcg gtgatgtgtt
     1261 ccgccgaaac cgccgtcggc gcgtatccgt ttgaaaccgt cagccaaatg gcgattatct
     1321 gcgcggctgc ggaaaaagaa caggattcgc tcaacggcgt tgccgaacag acagagtatc
     1381 ccgaagccgt cagcaccaac ttagcgattg ccggcggcgc ggtcagcgtg gcgcgcgcgg
     1441 ttcacgccaa agccatcgtc gccctgaccg aaagcggttc gaccgccttt gaaatcagcc
     1501 gccacaatat taccctgccg attttcgcgc tgaccccgag cgtttccgcc caacgccgta
     1561 tggcgatgta ccggggcgtg cgcccgctga ttttggcaac cagcaccgac cacgacacgg
     1621 cgttgaacga agtcgaaacg atgctggtgg aacacaacat cctgcattcc ggcgaccagt
     1681 acatcatcac cagcggctcg caaatgcgcg aatccggttc gaccaatacg ctggaagtgc
     1741 tgcgcgtcaa ataatccgcc ctgagtggaa aatgccgtct aaagccgata ctgtcgggct
     1801 tcagacggca ttttatttgc attccgtcag gtaaaaccga aagaattgtg caaatccggc
     1861 aacatcggat aaaatcgagt gcctatacta aagcgaaaca aggcatttcc gactgccttt
     1921 tttatttgtc caccgtccgc ctttttacgg aaaccgaaat gacccctttc acactgaaaa
     1981 aaccgtcctg ctgctcggca ctgcctttgc cgccgcatct gtccacgcat ccggctacca
     2041 cttcggcaca cagtcggtca acgcgcaaag cacggcaaat gccgccgacg cgtcgaccat
     2101 cttctacaat cccgccggcc tgaccaaact cgacagcagc cagatttccg tcaacgccaa
     2161 catcgtgctg cccagcattc attatgaagc agattccgcc accgacttta ccgggcttcc
     2221 cgtccaaggt tctaaaaacg gcaaaatcac caaaaccacg gtcgcacccc acatttacgg
     2281 cgcatacaaa gtcaacgaca atctgaccgt gggcttgggc gtgtacgtcc ccttcggctc
     2341 tgccaccgaa tacgaaaaag attccgtgtt gcgccacaac atcaacaaac tcggtctgac
     2401 cagcatcgcc gtcgaacctg tcgccgcgtg gaaactcaac gaacgccatt ccttcggcgc
     2461 aggcatcatc gcccaacata attccgccga actgcgcaaa tatgccgact gaggaatccc
     2521 aaaaaaagcg caaatgctgc aagcaacacc ttctaatcct actgccgctg ctcaaatcaa
     2581 ggccgacgga cacgccgatg tcaaaggcag cgattggggc gtcggctacc aactggcgtg
     2641 gatgtgggac atcaacgacc gcgcgcgcgt gggcgtgaac taccgttcca aagtttcaca
     2701 cacgctcaaa ggcgatgccg aatgggcggc agacggcgcg gcggcgaaac aacagtggaa
     2761 tgacaatatg ctcacaccgc tcggttacac ggcgaatgaa aaagcccgcg tcaaaatcgt
     2821 aacgcctgag tctttgtccg tacacggcat gtacaaagtg tccgacaaag ccgacctgtt
     2881 cggcgacgta acttggacgc gccacagccg cttcaataag gcggaactgt tttttgaaaa
     2941 agaaaaaaat attgctaatg gcaaaaaatc cgaccgcacc accatcaccc ccaactggcg
     3001 caacacctac aaagtcggct tgggcggttc ttatcaaatc agcgaaccgc tgcaactgcg
     3061 cgtcggcatc gcttttgaca aaccgcctgt ccgcaacgcc gactaccgca tgaacagcct
     3121 gcccgacggc aaccgcatct ggttctccgc cggcatgaaa taccatatcg gcaaaaacca
     3181 cgtcgtcgat gccgcctaca cccacatcca catcaacgac accagctacc gcacggcgaa
     3241 ggcaagcggc aacgatgtgg acagcaaagg tgcgtcttgc gcacgtttca aaaaccacgc
     3301 cgacatcatc gacctgcaat acacctacaa attcaaataa acgttacacc gtttgaatat
     3361 aaaaatgccg tctgaaacag atttccgttt cagacggcat ttttatccgg cagccgatac
     3421 gcctatctcg gtattcggga gaatatatac agaacctcct tcatatcaaa atggtaggga
     3481 cgctcgggca acaggacggc ggcaggcagc ttggtgatgc cgttcaaacc gcgcgggaac
     3541 agcgcaatcc gctctttgcc cttttccgca taatctgctt tgcggttgtg gatggaaact
     3601 acttcgcgat ggcggtaaag cagcgtgttc atttcgatat aggcttcgtt gtggatggct
     3661 gccgactgtt tctgcaacac gtcaatctgc ctgttgagct tgtcgttggc tttcatcagc
     3721 ctgttgaaga acgacggttg ctcctgcagt ttggcggtac aggcttcgat actttccgat
     3781 accggcccga actgttcgag gtatttttcc agacagtttt ccaagtcggt caggcagccg
     3841 gccatcttgc gggaaaattc ttcttcaggc tttccctgcc gaatggacaa tatagcggat
     3901 taacaaaaat caggacaagg cgacaaagcc gcagacagta caaatagtac ggcaaggcaa
     3961 ggcgaggcaa cgccgtaccg gtttttgtta atccactata tttgggaggg gcgtaacccc
     4021 ttccgaatca ggatggcaca tagggcgacg ctttatgtgc cgtcctgtgt gttgaaacat
     4081 cagcatagga aacacaggaa aagccggctg ttttgcgcct gcgtaccgta ttggtcaaaa
     4141 tgccgtctga aaccgacaac agggtttcag acggcatttc cgtcaatttt ccctttatct
     4201 gccgaacagg ttggcgatat tttccatctg ttcttcggta aaaccgtttt cgcccaatct
     4261 ggcggcaact gcccattcgc ccgacaaacc gtggtccgca ccgagctgcc gccatttttc
     4321 gactttggct tcggctgtgg ttttttcggg cagcggtttg acgggaatcc tgcttttggg
     4381 gacggtattc atcggtttgc cctaatcgat ggaatagccg cgaagatact gtatccgttc
     4441 gcgcgtcatc cgcgtgtcgc attggagctt gaggtattcg gcgtattcct gccggtctgc
     4501 ctgcgcggcg gcttgtcggc agttgctgat tttttcctgc gcccacttgc gctgttcgcc
     4561 gaccaactct ttttgcacgt cggtatcgag tcctccccaa agtttggtaa tttcggattc
     4621 cgcacgttgg ttttgtacgc gcgcctcttc cacttcgccc cgtgatacgg taacggtatc
     4681 ggcacgctcg acgtcgtcgg gatgcaggat ttcgggttcg ggcgcgcctt ctgcggcttg
     4741 gggtacgccc gcatcgccgc cggcggcatt gtgttccaaa atgtcttcgg gggtgggttt
     4801 ggacggttct tcttcacggg ctttgccgct caaaaccctg accgcgtctt cttttgtcac
     4861 cgccttgccg tctatcatca cgatactctt cacgccgtaa ggcagcaacg cggcagacag
     4921 cgtttgcgtc gccataccga ccgtgttgtc gataaatgcc gtccgagcgt ctttggcggg
     4981 caggaagcgg acggctgccg tcaatacgcc gtctttaaac tcgacattgc cgcccgtctt
     5041 ctgctgcacg atgtctgcca aagacgtttc cccatacagc agggggctgt ttgcctcggc
     5101 atcggcaagc gtttcagacg gcacggtaat gttcaaatcg gcgatacaga acgtgcgccc
     5161 gccttcctgc gtttccgaag cgtgttccaa agaaaacgcc aaaccgtagg cggcggcgat
     5221 aattttgtcg gcatcgacaa actgcctgcc gtcttcgcgc gcgaaagaac gcgcttcctg
     5281 cgtgagcgtt tcctgaatac tgccgcgtat gtcctgcaac acggcggggt tggcgcattc
     5341 caacgccttg ggcggttctt ccctgccgca cgctgcaagc agcagggcaa acggcagcgc
     5401 aatgagtttc cgatacatga tgttccttcc aaacggtgca gaccgatggc ggctatcata
     5461 gcaaaagcac ctgcaggcgg taaaccttgc aggcgctttg tcggaaaggc ggaaaatcag
     5521 cctttaaaga aattcacaaa accggtgaga atcgcggcat taatcaaatc gacaaagaac
     5581 gcgccgacca taggcacaat caaaaacgcc ttatgcgacg cgccgaaagt atgcgtgacg
     5641 gactgcatat ttgccaccgc cgtcggcgtt gcgcccaaac cgaaaccgca gtggccggca
     5701 gccaatactg cggcatcata gtcgcgcccc ataaagacat aggtaacaaa agtcgcgtac
     5761 aaaaccatca ctgcggtttg tactgcaaga atcacggtta cagaccccgc caaaccggtc
     5821 agctcccaca gtttcaaatt cagcaacgcc attgccaaga aaagcgaaag cgaagcattg
     5881 ccgaacacat cgatggcacg gtcgaacata ttgaccttga atgcggcggt aaggatgttg
     5941 cggataacca cgccgccaaa cagacaccac acgaatttgg gcaggtcgaa caggtattct
     6001 ttgtcgaagc cgtccataat ctcggcaaac gccagacacg cggcaaacat ggcaagcgtt
     6061 tcaacggcag attccgccgt aatcaggcgg gtgcgttttg cctgctcgaa cacgtcgtcc
     6121 gcgttgtcgt cctgatcctg ttttgtgttt tcaaccggtt tgcggcccat tttgttgatc
     6181 aggcggcgcg caaccggccc gccgatcagg ccgccgaaca ccagcccgaa agtagccgaa
     6241 gcaataccca aaccggttgc gccgaccaag ccgtattgcg tttcaaaatt aggtccccac
     6301 gcacctgacg tgccgtgtcc gcccgtcagc gacaccgaac cggtaatcag accgatgagc
     6361 gggtccaaac ccaaagccgt agccagtccg accccgacaa agttttgcac caagataaat
     6421 ccgcccacaa tcgcggtaaa aaccaccagc ggcaaaccgc ccgccttcaa acgggaaaaa
     6481 tccgcgctca agccgatgga cgtgaagaaa atcagcataa acgcattttg cagcggtttc
     6541 tcaaatttga agctcacgcc gtacgcctcg tgcagcgcaa acaggataat cgcggcaatc
     6601 agcccgcccg ccacgggttc ggggatgtta aaatcacgca agattttgat tttcttaacc
     6661 aaaaccttgc cgaccaacaa aaccaaagtg gcggcaatca aggtgtaata actgttaaac
     6721 gcccattcca tatcgtttga cctcctaaaa tgtttttctt tatatccgcc cgaaggggac
     6781 gcaagcgcga agatattagg ggaatagtta aggcttgtca aagaatcgcc gttttttata
     6841 gtggattaaa tttaaaccag tacatcgttg actcgccttg ccgtactatc tgtactgtct
     6901 gtggcttcgt cgccttgtcc tgatttaaat ttaatccact atacaactat tttttacaaa
     6961 caaaatgccg tccgaaactc cgttcagacg gcatcatggg aatacacata tttgaaataa
     7021 cagaaaaagc gggggcaaaa cccgccgctg catctgtcgc tgtacatccc tgctgtcgga
     7081 cttaccgctt caacttcgca aacgcatccg ccatcgccga attggttggg acacgctcgt
     7141 tgcgttgtgg tttgcggccg gctgtccgtt cgcggctgcg gtttttccag ctcggacaga
     7201 atgtccagaa tggtcggcag tccgaagtgt tcgtcggtga agtcggacgc tttgatttgc
     7261 ttcacgcgct cgcggttaac tgcctaaata tgaaattctt ggtcttcttg tagcataagt
     7321 tctcgatgat atgccaagta agatttacgt ttgtcgttga gcagattgtt atctagcttc
     7381 acatcacacc aacgtcgcca aacatcatct gataaccgct tagatttcac catatttcct
     7441 tcatcatcaa acgaaatata tttcaaatca aataggctat ccaaggtacg acttaatagc
     7501 aagccgttat ttgggtcgta tgcttcggta tcatccgaca ggatgaaagg cttaatatga
     7561 ctggcaatca atacaggata ggcaagtttt tccagcatac atttcacatt accgtaatgt
     7621 tcttcacttt cttcttgaag ttggtttttg tataggcgat gtaaataagg gtcgcgtttc
     7681 cttacagtaa tttcatccaa attaccaaaa atacgttggg catcctcagc aaaatataag
     7741 tcatcaccta ctcgttttaa gtcgtctaat ttgtctaata ggttccataa ataacttatt
     7801 tgattgtatt ttcgttcaat aaatcctgat tctattccct gttgaaaata atcattcaat
     7861 tctgtttcgg ttagataatc ttgttgaaag gtttttaagt ctactaacat catggcggca
     7921 atttcttttt tatttagctt gccttgtgga tgctcgacca gcgttttaat caggaaattt
     7981 atttgtcgaa tattagagtt ttcagttact gaacgctgaa agccagagtg cgtatatacg
     8041 attttggaaa gtagggtttg tcgttttcgg ttagtacgag cttgtacata atctcgtgat
     8101 aaaggtgtat agcttgataa aaacggttct ataaatccca tttttacaaa ttgattaata
     8161 gctttacgaa ccgaggccaa attcatggcc ttgcttgttc tctgcgaagc cataacggaa
     8221 tcttgggcaa tgttttgctg caatgcttca tatagttcgt tgctgtatgg ctggtcttga
     8281 ttcgcatcaa taaagtcaag acaaatattg agtgcagtac aaaatttttg atcgttataa
     8341 tttgtaaagg cattggtaaa cgcccaataa tcttcataaa tttggctgtt tggctgatta
     8401 ttactcatct ctgtctccaa gaatattcga taccaatatt ctgtttttgt atttaagtaa
     8461 tatctatcat tcatgtattt tctactaaaa acagaatttc cttatggctt tgcttctttt
     8521 cttttcctgt aaccttataa acgtgaggcg tttccaagat ttgtacattt cgcccatttt
     8581 tacctaacat ttcttctaat tcatctcggt ttggatatga agcattgttg tagcttaaca
     8641 gccaatattt aaatggtttc aaatgattga ttaccctttt aaataattcg acagcctgct
     8701 ttttatccat gaaatgattg gcaaatggtt tgggtatgga tgatgtaatg taactgtcca
     8761 ataatccgta aaagccaaaa taattgttca tcgtgcctgt gtaaggtggg tctaaataga
     8821 cggcatcagc ttgtatatta ggtaataaat caaatatatc ttgattaaag gcgtgatgcc
     8881 gccagccatt attgaaaaca gcttggttat agtcatccaa attttgcaga aaatgacttt
     8941 gaaaactttc attgtggtag gcgcgtcgac ggccatattt agcataactg tattcttcat
     9001 cacgcagttg tttaactttt tcccaaggaa ttgtgaatcg tgaatagggc attttacgta
     9061 tcatggcgcg acgcattaaa gtaaacgcca aagcacgttt atactggtta ttcagcttcc
     9121 ctatattttt acggtacaaa tcaagttgtt ggcattcatc gtgaaaatag aatttttcgg
     9181 aataacgctg actcataaaa ccggcatgcg ggctgcctga aaaaatcatt gcgacatcat
     9241 cgtcatttaa tgtttcatgg ttgttttcta ttaatgctaa agcaatttgg taattaattg
     9301 ccaaaatatc gttagtaatc acacgatagc cgcgtttttt ggcttcgtag gcaaaggaac
     9361 agcctccact aaatacatct gcaactgtat ctacatcaga cggaagctgg tcacaaatcc
     9421 aggaagctat tttctcttta ttaccgatat agttaatttt cggatatttc ttattcattt
     9481 tcactcattt ttaaaatact ttcagcaata gcttttgcca ataaaggcgg cacggcgtta
     9541 ccaacctgct gctgctgggc aattttgctg ccgcaaaaaa taaaattatc agggaaagat
     9601 tgtaaggcag ctaattcacg aacggttaaa gcccgattct gttcatagtg aaaaactttg
     9661 cgcatatctc ctgtaataca aacggctggt ttgttgctgt tgtaacggat gtatttacgg
     9721 atatcacctg ttttcggacg taatggttca ggaatatcgt tacggttacc tccattttta
     9781 acaaatgcca ttttttctaa catttgtgcc gaatgattca tagcttcatg atttgcaacg
     9841 tgtggattgc tttcgccagc agccagtttt ggaaaatgtc ctattgctga tccaacagtc
     9901 tgatgggaaa tctgcaaagg ttcgggaaag gaaattttgc ctttatccct cctcccgata
     9961 aatatcactc ggctacgtat ctgaggaaca ccgaaatcgg ctgcactcag tatcttacat
    10021 tccaccgaat aaccgatatt ctgaaatgct tgaataatct caatacgtgt tttacctgaa
    10081 ttgtgtgtat agagtcgcgc tacattttcc ataacaaaaa aatatggttg gacaatttta
    10141 actattcgga caaactcttt aaataaatgg ttgcgtgggt catctgtaaa tgtccgtcca
    10201 atctttcctg ccatactaaa accttgacaa ggtggtcctc caataaccaa atcaactgat
    10261 tgtccgttaa gacaattggt taaatcttgt tcggttagtg tggttaaatc tttttgcagt
    10321 aattgatgac gggggaagtt ggtacggtaa gtctgacaat aatcagactc catttcaaca
    10381 gaaagcaatt gttggaatcc ggcttgttca aaacccaagg ataggcctcc tgctcctgag
    10441 aaaaggtcaa tataggtaag tggtgtgtgc ataagtcaaa atccataaac tcttcgatta
    10501 ttttaccgct tcaacttcgc aaacgcatcc gccatcgccg aattggtcgg ggcgcggtcg
    10561 ttgcgttggg gtttgcggcc ggctgtccgt tcgcggctgc ggttttcaga cggcatttta
    10621 tgttttgcgc cgcccggttc gtcatccaag cgcatggtca gcgcgatgcg tttgcgtgcg
    10681 gcatcgactt ccagcacttt cacttttacc acgtcgccgg ctttcaccac gtcgcgcggg
    10741 tcttggacga acttattaga cagggcggag atgtgcacca agccgtcctg atggacgccg
    10801 atgtccacga acgcgccgaa gttggcgacg ttggagacca cgccctcgag tatcatgccg
    10861 acttgcaagt cgctgatttc gtggatgcct tcggcaaacg atgccgtctg aaacgcaccg
    10921 cgcgggtcgc ggccgggttt ttccagctcg gacaggatgt ccaaaatagt cggcagaccg
    10981 aagcgttcgt cggtgaagtc ggatgctttg atttgcttca cacgctcgcg gttgccgatg
    11041 agttcggcgg cggtaatgcc ttgttgcgcc agcattttgg cgacgacggg ataggcttcg
    11101 gggtggacgg cgctcgcgtc cagcggctct ttgccgccgt taatccgcaa aaagcctgct
    11161 gcttgctcga aggttttttc gcccaagcgc ggtactttta gcaatttttt tcggctgtcg
    11221 aacgcgccgt tttcatcacg gtaggcgacg atgttttggg caagggtttg attcaagccg
    11281 gaaatccgcg ccaagagcgg ggcggaggcg gtgttcgcgt ccacgccgac ggcgttcacg
    11341 cagtcttcga ccaccgcgtc cagcgatttg gcgagccggc tttggttcac gtcgtgctga
    11401 tactgcccca cgccgatgga tttggggtcg attttgacca actcggcaag cgggtcttgc
    11461 agcctgcggg cgatggacac tgcgccgcgc agggaaacat ctaagtcggg aaactcgcgc
    11521 gccgccagtt cggacgcaga ataaatcgac gcgccggctt cggaaacgac gattttgtgc
    11581 aggctgcttt cgggcattcc gcgcaccagt tcgcccgcga ttttgtcggt ttcgcggctg
    11641 gcggtgccgt tgccgatggc gatgagcttc acaccgtgtt gtttaatcag gcgcgacaac
    11701 gttgccaaca tattgttttc ttgatgcaaa tagacaatga ccgtatccag cagtttgccc
    11761 gtgtcgtcca ccacggcgca tttcacgccg ttgcggtagc cggggtcgag tcccaaagtg
    11821 gtcagccgcc cggcgggcgc ggcgagcagc aagtctttga gattgcgggc gaacacggta
    11881 atcgcgtcag tgtcggcagc ttctttcaaa cgattgagag cttcgagttc cagcgacaaa
    11941 aagattttcg cgcgccaagt caggcgcacg gtgtcgcgca gccatttgtg gccgtctgaa
    12001 accttgaagc ggcgggcgat gatttgctcg tattcgcttt gctgcgtaat cggcgtgtcg
    12061 tcgggctggt atttgagcgc gatgttcaac acgccttcgt tgcgaccgcg caaaaccgcc
    12121 agcgcgcggt ggctgggcat ggcgcgcacg ggttcgcggc ggtcgaaata atcgctgaat
    12181 ttttcgcctt cggtttcttt gccttcaacg acttgcgtgt ggatttcggc ttcgttccac
    12241 agcttgtcgc gcagcgtgcc gatgagttcc gcgtcttcgg caaactgctc catcagaatc
    12301 gcgcgcgcgc catccagcgc ggctttggcg tcggggatgt tttcgttcag gtagccttgc
    12361 gcggcggctt ccacgtcctg cggctgttcg gcaagcaaca cgtccgccag cggctgcaaa
    12421 ccgtgttcgc gagcgatttg cgctttggtg cggcgtttgg gcttgtaagg cagatacagg
    12481 tcttccagcg cggttttgtt gtcggcggct tcgatttgcg ccctgaggtc gtctgaaagc
    12541 ttgccttgct cttcaatgct ttttaacaca acggccttgc gctcttccaa ctcgcgcagg
    12601 tattgcagcc gctcggcaag ctggcgcagc tgcgtatcgt ccagcccgcc cgtggcttct
    12661 ttacggtaac gcgcgataaa ggggacggtc gcgccgtcgt ccaaaagctc gatagcggca
    12721 ttgatttgca cggtagtggc ggagagttct tgggagagga ttcgggtgat gttcataaat
    12781 tgcctccgtg atgccgtctg aacagccgac ggcgcaatgt gggtaatcgt ttggaaaaga
    12841 tgaagataat actgatatac tttgcccgat acaatctgaa aggaatgttt atgaccgtcc
    12901 tgattaccgg cggcaccggc tttatcggtt cgcacaccgc cgtctcgctc gtccaatccg
    12961 gttacgatgc cgtgattttg gataatctgt gcaactcgtc tgccgccgtc ctcccgcgcc
    13021 ttcggcaaat taccggcaga aacataccgt tttatcaggg cgacatccgc gactgtcaga
    13081 ttttgaggca gattttttca gaacatgaaa tcgaatccgt catccatttt gccggtttga
    13141 aggcagtggg ggaaagcgtt gccgagccga caaaatatta cggcaacaat gtttacggca
    13201 gcctggtgct ggcggaagaa atggcgcgcg cgggcgtgtt gaaaatcgta ttcagctcgt
    13261 cggcaaccgt ttacggcgat gcggaaaaag tcccctatac ggaagatatg cgcccgggcg
    13321 ataccgctaa tccttacggt gcgtccaaag cgatggtgga gcggatgtta accgacatcc
    13381 aaaaagccga tccgcgttgg agcgtgattt tgttgcgcta tttcaacccg atcggcgcgc
    13441 acgaaagcgg acttatcggc gaacagccca acggcgttcc caacaatctt ttgccctata
    13501 tctgtcaagt ggcttcgggc aggctgccgc aactgtcggt attcggcggc gactatccga
    13561 cccccgacgg tacgggaatg cgcgactaca tccatgtgat ggatttggca gaagggcata
    13621 tcgcggcaat gaaggcgaaa ggcggcgttg ccggcgtaca tttgttcaac ttgggttcgg
    13681 gacgcgccta ttccgttttg gaaatcatcc gcgcctttga ggccgcatcc ggtttgcaca
    13741 ttccttaccg aatccaaccc cgccgcgccg gcgacttggc gtgttcctat gccgacccgt
    13801 cccataccaa acaacaaacc ggctgggaaa ccaaacgcgg cttgcagcaa atgatggaag
    13861 attcgtggcg ttgggtcagc cgcaaccccg gcagatatgg ggattaaatg caaaccgcag
    13921 gcaaaaaaaa catcctcgtt accggcggcg cgggttttat cggctcggca gtcgtccgcc
    13981 atattatcca aaacacccga gattccgtcg tcaacctcga caagttaacc tacgcgggca
    14041 atctcgaatc gctgaccgac attgccgata atccccgcta cgcttttgag caagtggata
    14101 tttgcgaccg cgccgaactc gaccgcgtgt tcgcgcaata ccggcccgat gccgtgatgc
    14161 acttggcggc ggaaagccac gtcgaccgcg ccatcggttc ggcaggcgaa tttatccgaa
    14221 ccaatatcgt cggcacattc gacctgctgg aagctgcgcg cgcctattgg cagcaaatgc
    14281 cgtctgaaaa acgcgaagcc ttccgtttcc accatatttc taccgatgaa gtctatggcg
    14341 atttgcacgg cacagacgat ttgtttacag aaaccacgcc atacgcgccg tccagcccct
    14401 actccgcctc caaagccgcc gccgaccacc ttgtccgcgc gtggcagcgg acttacaggc
    14461 tgccctccat tgtcagcaac tgttccaata attacggccc ccgacaattc cccgaaaaac
    14521 tcattccttt gatgattctg aacgcacttt ccggcaaacc actgcctgtg tacggcgacg
    14581 gggcgcaaat ccgcgactgg ctgtttgtcg aagaccacgc gcgcgcgctg tatcaggtcg
    14641 ttaccgaagg tgttgtcggc gaaacctaca atatcggcgg acacaatgaa aaaaccaatc
    14701 tcgaagtgat caaaaccatc tgcgccctct tggaagaact cgcccccgaa aaaccggccg
    14761 gcgtggcacg gtatgaagat ttaattactt tcgtacaaga ccgccccggc catgatgccc
    14821 gctacgccgt cgatgcggcg aaaatcaggc gggatttggg ctggctgcct ttggaaactt
    14881 ttgaatccgg cctccgcaaa accgtgcaat ggtatttgga caacaaaacc cggcggcaaa
    14941 acgcataaaa cggcaactgc cgttcggaac gcttaggtac gggcaaatag ttttcagacg
    15001 gcatcccgac gcaatgccgt ctgaaaaccc atcgcaaagg aagaaagaaa agatgaaagg
    15061 catcatactg gcaggcggca gcggtacgcg cctatatccg attacgcgcg gcgtatccaa
    15121 acaactgctg cccgtgtacg acaaaccgat gatttattac cccttgtcgg ttttgatgct
    15181 ggcgggaatc cgcgatattt tggtaattac cgcacccgaa gacaacgctg ccttccaacg
    15241 gctactcggc gacggctccg acttcggcat ccgcctgcaa tatgccgttc aacccagccc
    15301 cgacggcttg gcgcaggcat ttatcatcgg cgaagaattt atcggcaacg gcaatgtctg
    15361 cctgattttg ggcgacaata ttttctacgg tcaatcattt acgcaaacat tgaaacaggc
    15421 cgccgccaag acccacggcg caaccgtatt cggttatcgg gttaaagacc ccgagcgttt
    15481 cggcgtggtt gaatttgacg aaaacttcaa cgccttatcc atcgaagaga aaccgcaaca
    15541 gcccaaatcc gattgggcgg taaccggctt gtacttccac gacaaccgcg ccgtcgagtt
    15601 cgccaaacag ctcaaaccgt ccgcacgcgg cgaattggaa atttccgacc tcaaccggat
    15661 gtatctggaa gacggctcgc tgtccgttca aatattggga cgcggtttcg cgtggttgga
    15721 caccggcacg caagaaagcc tgcacgaggc ggcttccttc gtccaaaccg tgcaaaatat
    15781 ccaaaacctg cacatcgcct gcctcgaaga aatcgcctgg cgtaacggct ggctgacgaa
    15841 aaaagatgtg gaaacacggg caaagccttt ggaaaaaacc gcctacgggc aatacctgct
    15901 gcgcctgatc ggcaaataaa taccgtctga acgccccgac ggctttcaga cggcatccaa
    15961 accgtgccgc gccgccacct taaccgacct gccgcacggc gcgcataaaa aatcaaaccg
    16021 caaaaaacag cggtaacgat accgccaaag gactttatgg acatcatcga caccgccctc
    16081 cccgacgtaa aacttttaaa accgcaagtc ttcacagacg ggcgcggttt ttttatggaa
    16141 acattccgcg acggatggtt caaagaaaat attgccgatc gaactttcgt gcaggaaaac
    16201 cactccaact ccagcaaggg cgtattgcgc ggcctgcact accaaaccga aaacacccaa
    16261 ggcaaactcg tacgcatagt tgtcggcgaa gtgttcgacg tggccgtcga tatgcgcgaa
    16321 ggttcgccga ctttcggcaa atgggcgggc gcaaccttgt cggcgcaaaa ccgataccag
    16381 ctttggatac ccgaaggctt cgcacacggt ttttgcgttt tgggcgacgc ggcggaagtc
    16441 gtgtacaaat gcacggacta ttacaaccct gaaaccgaac aggttttaat atggaacgac
    16501 ccggcaatcg gcataggctg gccgcttcaa accgcgccgc tactttcgcc caaagacctt
    16561 gccggcaaaa cgtgggcaca agccgaaaag ctccgcctta cgctttcccg ataaaaaatg
    16621 ccgtccgaac gtttcagacg gcattttctc cgacaggcta cctacccgcc ttcagtacgc
    16681 gctgtgcaaa gaaaaacatc ccggtaacga agaacgccaa gcccaaccaa gaagcggcgg
    16741 catcccagtt ttgcagattc aacgcgagcg gcgcatccga gccgccgttg gtcacagaga
    16801 aggcaataat aaacgccata attacaccaa tcaggctttc accgacaatc aggcctgccg
    16861 cgaaaagcgt accgatacgg tcggcgtttt tcagacggcc ttcgcggttt tccgccttct
    16921 tgccaatgat gtgtttcaac accgccgcca acaccgcgcc tgccacgatg ggcatattga
    16981 cggacggcgg cagataaatg cccataccga ccgcaaggac gggcagggca agtttaccgc
    17041 ctgatgattt tttcaacacc aaatcgacga cgattaatac cgctccaatc gcgataccgg
    17101 taaagatgta cgcccattcg aggttgtggg cgaaaatgcc cgacgcaatg gtcgtcatca
    17161 aagtcgcttg aagggctgcc aaagcctgcg ccgcatccat gccttcgcgc ggcattgcgc
    17221 cggtaaagcc gtaggcttcg taaagcagtt ccaacacggg cgaaataacc agcgcaccaa
    17281 cgatacagcc gataatcagg gcgacttgct gccgccaagg cgtagctttg agcaggtagc
    17341 cggttttcaa gtcttgcagg ttgtcgttgg aaatcgaagc cacgcagatt actgccgatc
    17401 cgcaaaaaag cgtcaacgcc agtaagaatt tgcggttggc ctcatccgcc aacaaacctc
    17461 cggattcgcc taccagcagc aaaaccagtg aaataacgac gatggacacg atgcccacgc
    17521 cggaaatcgg gctggaagac gagccgacca aacctgccat ataaccgcag gcggcggcga
    17581 ccaaaaagcc gatgacggaa gccaaaagcg tgcaaaccgc caccaaaagc cacgccatgc
    17641 cgcccgtaat gtgcgaatcg ccgataaagt ggtaaaacga cacgcctaga ataaacatca
    17701 tagacaacac ccagaaaatc atggctttag gcgacaaatc ctgttcggcg cgttctgtag
    17761 cgggcgcacc gccgccaaaa ctcttgaacg acatcttcat gccttccacc attggcttga
    17821 gcagcatcaa aagcgtccaa accgccgcga tacctatggt tcccgcaccg ataaaacgca
    17881 ctttttcctt ccacagcttc atcgcaaacg ccgccatttc catatcggaa ggttgcggaa
    17941 tgtgtgagga gaaatacggc acggcaatac cccaagcaat cgaaatgccc agcaggatgg
    18001 cgatgccgcc cgtcagtccg accaaataac ccgcgcccaa caaggccagt gaaaagccca
    18061 tcggcagttg gaaaattgcc gtaccgcttt taaaccaata actcgcgcta tcggcaatca
    18121 cacgcagacc tccggcgcaa aagctcatca atcccgccaa cgcgccgccg gccgccagct
    18181 ctttgatacc gctgccgccc tgacggtcat ccccttcttc atgaccgccc actttcaaaa
    18241 tttcagcagc cgccacacct tccggataag gcaaatcgct tttcaccacc atcgcgtaac
    18301 gcagcggaat ggtgaaaatc acccccaaaa tcccgccggc aatacataaa agcgtcgtct
    18361 gccaaaacgg gaaactgctc cagtagcccg ccatcagcaa accgggcagg acgaagatga
    18421 tggttgaaag cgtacccgca gccgaggctt gggtctgcac catgttgttt tccaaaatat
    18481 tgctgccttt gaaaaatttt aaaaccgcca tcgaaatcac cgccgccgga atcgacgagg
    18541 caaaggtcag cccgactttc aaaccgaggt aaacgttaga cgcagtaaaa attacagtga
    18601 tcaatgcgcc gagtatcatg cctcggagcg tcagttcgcg gtattcttct accgaaccgg
    18661 ataaagattt attcattatt cttcctttga caacagacgt tcacatattg ttggcatcac
    18721 gccatgatgt caagttttaa aaagaacagt taaaaacaat tatcccaccc tgcccatacc
    18781 caattgaaaa taaaaactat ttccaaacga acaaaaacag ccgtatcagg gaggttcccc
    18841 gatacggctg cttgtttccg aaccttaaaa tcaatcaaac aaatcgcgca gcttgtctaa
    18901 aaacgatttc ttgcgcggcg tttggttttc caagcccgta gaaatccgct caaattcttc
    18961 caaaagctct ttttgacggt cggtcaaatt gacaggcgtt tcgacaacaa tatggcagta
    19021 caaatcgccg gtcgcgctgc tgcgtaaaga tttgacaccc ttacctttca cgcgcatcct
    19081 cctgccggtt tgggtttctt tggggacggt gagcttgact ttgccgtcca aggtcggcac
    19141 ttctaactcc ccgcccaaag cagccgtggc aaaactaatc ggcagttcgc aatgcaaatc
    19201 caaaccatcg cgttggaaaa tcttatgcgc ccgaatgcgg acggttacat acaggtcgcc
    19261 ggcaggcgcg ccgtgcatac ccggcccgcc ttcaccgctc aaacggatac gctgcccgtc
    19321 atcgataccg gcgggaatat tgacttccac cgtcttgacc gccttattcc gccccacgcc
    19381 acggcatttg acgcaaggtt ctttaatatg tttgcccgca ccgcggcagg tcggacaagt
    19441 ctgctgcata cggaaaatcg cctgctggat gtgcaccgta cccgaacctt tgcaagtcgg
    19501 gcaggtttcc ggggatgccc ccggtttcgc gccgctgccg ttgcagacat cacacgcttc
    19561 ataagtcgga atattgatgc gtttcttcac accttttgcg gcttcttcaa gcgtgatttc
    19621 gataccgacc tgcacgtcct caccctgata atcaggctgg gcgcgccccg aaccacctcc
    19681 aaacatttgg ctgaaaatat ccccaaagtc aaaaccctgc gcaccgccaa atccgccaaa
    19741 ccctccgaag cccccctgtc cgccgccttc aaacgccgca tgaccatact ggtcgtacat
    19801 ggcacgcttt tccttgtcgg acaaagtttc atacgccttt tgtacttctt taaacttctc
    19861 ttccgcctct ttattgtcag gattgcggtc gggatggtat ttcatcgcca atttacggta
    19921 ggctttttta atctcatcat cggtagctgc tcttgccaca cccagcgtcg cataaaaatc
    19981 ttgattactc attttttcat ctaaaaataa aatcacggct taaaatgagg gcaattgcgc
    20041 aaaacacaag acaaacagac tgagaccttt gcaaaaatcc cc
//
LOCUS       DAWTHD010000024        19495 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWTHD010000024 DAWTHD010000000
VERSION     DAWTHD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19495
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.024"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            6..81
                     /locus_tag="WM704_000709"
     tRNA            6..81
                     /locus_tag="WM704_000709"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WM704_000710"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WM704_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFA9340180.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WM704_000711"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WM704_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFA9340181.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WM704_000712"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WM704_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFA9340182.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WM704_000713"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WM704_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFA9340183.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2470..2970
                     /gene="rplJ"
                     /locus_tag="WM704_000714"
     CDS             2470..2970
                     /gene="rplJ"
                     /locus_tag="WM704_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFA9340184.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3028..3399
                     /gene="rplL"
                     /locus_tag="WM704_000715"
     CDS             3028..3399
                     /gene="rplL"
                     /locus_tag="WM704_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFA9340185.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            3590..7768
                     /gene="rpoB"
                     /locus_tag="WM704_000716"
     CDS             3590..7768
                     /gene="rpoB"
                     /locus_tag="WM704_000716"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFA9340186.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(7733..7873)
                     /locus_tag="WM704_000717"
     CDS             complement(7733..7873)
                     /locus_tag="WM704_000717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340187.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7921..12096
                     /gene="rpoC"
                     /locus_tag="WM704_000718"
     CDS             7921..12096
                     /gene="rpoC"
                     /locus_tag="WM704_000718"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFA9340188.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12385..12798
                     /locus_tag="WM704_000719"
     CDS             12385..12798
                     /locus_tag="WM704_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340189.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGN"
     gene            12816..13445
                     /locus_tag="WM704_000720"
     CDS             12816..13445
                     /locus_tag="WM704_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340190.1"
                     /translation="MSEQKIEFYGADKEQNYALLKTWVYEQPYSVVRGYFGYSRKDGN
                     PIEEDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSFQYEGRAFGGNGVLSKESLDNH
                     NGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIERIPYRESGSSLGLKDRVSYFGV
                     NEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHKHQAVIGFGGEKK"
     gene            13683..13925
                     /locus_tag="WM704_000721"
     CDS             13683..13925
                     /locus_tag="WM704_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340191.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            13837..14826
                     /locus_tag="WM704_000722"
     CDS             13837..14826
                     /locus_tag="WM704_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9340192.1"
                     /translation="MCRRFAMQCRLKFLKARQAAMFEAADLYRELLSERPDLVYPRFD
                     LGVMLFEDKQYREALVQLHRAEEVLPPDMRQLAREYIRQAEAVQAWHPSFNMNYEQTD
                     NVNNASLSRDIVINGRKWIKSEDSLPKRANGIRYELGIDRMFNMAGNHFARLGISGSG
                     VHYWNARDFSEQAFHAEVGYRYRNSRLEWGFRPFVKQNRLGNNRYTANTGIALDYSRR
                     LNEKWRSTQSFQYGRKQYHDEYLAKRYNSKTISVSGTFSYYAMSAWQLYGGISGMFDN
                     TVEKEQASRRYGVSLGTVKILDGGLGLKLGAGYTKRIFKAPATLIYNFTRRDD"
     gene            14833..14982
                     /locus_tag="WM704_000723"
     CDS             14833..14982
                     /locus_tag="WM704_000723"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9340193.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKNNSNMPAFYSHSGKGWFVS
                     MEKTY"
     gene            15161..15532
                     /gene="rpsL"
                     /locus_tag="WM704_000724"
     CDS             15161..15532
                     /gene="rpsL"
                     /locus_tag="WM704_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFA9340194.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            15650..16120
                     /gene="rpsG"
                     /locus_tag="WM704_000725"
     CDS             15650..16120
                     /gene="rpsG"
                     /locus_tag="WM704_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFA9340195.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            16139..18244
                     /gene="fusA"
                     /locus_tag="WM704_000726"
     CDS             16139..18244
                     /gene="fusA"
                     /locus_tag="WM704_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFA9340196.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            18332..>19495
                     /gene="tuf"
                     /locus_tag="WM704_000727"
     CDS             18332..>19495
                     /gene="tuf"
                     /locus_tag="WM704_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFA9340197.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT         5261 a         4294 c         5053 g         4887 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaatgttaa agtgaatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggagggttt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgccgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcga attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ttagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 tccccgtgtt tctggtttta ttggagggag ggctaataga cccacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gttggtcaac aggttcgtgt aaatgaagga ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgag aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggc aaagccaatc catccccccc agttggtcct gctttgggtc
     1201 aacgtggttt gaatatcatg gaattctgta aagcatttaa tgctgcaacc caaggtatgg
     1261 aatctggttt gccaattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac tcctccggct tctattctgt tgaaaaaagc cgccggtttg caaaaaggta
     1381 gttctaatcc tctgacaaac aaggtaggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtt cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggacg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagttcttc gctcttctgt tgaagctaac aaactgtacg caatcgacga
     1621 agcaattgcc ttggttaaaa aagctgctac cgctaaattt gacgaatctg ttgacgtatc
     1681 tttcaacttg ggcgttgatt ctcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggt actggtaaga caacccgtgt ggctgtattt actcaaggtg taaatgcaga
     1801 agctgctaaa gaggctggtg cggatgtcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgttgttat tgcttctcct gatgcaatgc gtatcgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaaa taggtacggt
     1981 tacccctaat gttgccgaag cggtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcgttcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggacgc tatcgttaaa gccaaacctg ctgctgctaa
     2161 aggtcagtac ctgaaaaaag ttgctgtatc cagcactatg ggtttaggtg ttcgcgttga
     2221 tacatcaagc gtgaataact aatctaagaa attttcaagc aactctgttt ttgagttgct
     2281 tgaatttggg ctacttaaaa ttaagtagat gtccaagacc gtagggatcg taagatttaa
     2341 tcgtaactgc cctacgcaga cggtagtcct gaaacacatt gcaagattgc ttgtaagatg
     2401 tctttttagg ttaccgcgct ggtgggatat cgttttggta tcctgtttat aaacagtggg
     2461 aggtagacct tgagtctcaa tattgaaacc aagaaagtgg cggtcgagga aattagcgcg
     2521 gcaattgcta atgctcaaac cctcgtagtc gctgaatatc gcggtatcag tgtttccagt
     2581 atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt atttgcgcgt tctgaaaaat
     2641 actctggctc gtcgcgcagt gcaaggtact tcatttgtag aattggccga tcaaatggtt
     2701 ggtccgttgg tttacgctgc ttctgaagat gctgttgctg ctgctaaagt gttgcaccaa
     2761 ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt cttacaatgg cgaagtaatg
     2821 aatgctgctc aggttgctga gttggcttct attccgagcc gcgaagagct gttgtccaaa
     2881 ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc gcggtttggc tgctttggca
     2941 gagaaaaaag ccggcgaaga agctgcttaa tcgattttgt ttctgttaat caattatttt
     3001 ttaatacaat atttggagta aaatagcatg gctattacta aagaagacat tttggaagca
     3061 gttggttctt tgaccgtaat ggaattgaat gacctggtta aagcttttga agaaaaattc
     3121 ggtgtttctg ctgctgctgt tgcagttgca ggtcctgctg gtgccggtgc tgccgatgct
     3181 gaagaaaaaa ccgaatttga tgtcgttttg gcttctgccg gcgatcaaaa agtcggcgtg
     3241 attaaagttg tccgtgcaat tactggtttg ggtctgaaag aagctaaaga catcgttgac
     3301 ggcgcaccta aaaccattaa agagggtgtt tctaaagctg aagccgaaga catccaaaaa
     3361 caactggaag cagcaggcgc taaagtcgaa atcaaataat ttgatgcttc ttatgaaggc
     3421 tggcagtttt ctgccagcct tattttgctt cttaaaataa gcattaagta ttgtttacat
     3481 ttatttgcat agtttttatc aaatcattgc aaataaatgt aaatatcaga ttgatgcgta
     3541 ccgttgtttt cagacggcct attattgaaa attacttttc ggagtgtgta tgaactattc
     3601 gtttaccgag aaaaaacgta tccgtaagag ttttgcaaag cgggaaaatg ttttggaagt
     3661 tccttttcta ctggcaaccc aaattgattc ttatgcgaaa tttttgcagc tggaaaatgc
     3721 ttttgacaaa cgtaccgatg acggtctgca agcggcattt aattctattt tcccgattgt
     3781 gagccacaac ggttatgctc gtctggagtt tgtatattac acgctgggcg agcctttgtt
     3841 cgatatccct gaatgtcaat tgcgcggaat cacttatgct gcccctctgc gcgcgcgtat
     3901 ccgtttggtg attttggata aggaagcgtc taaaccgacg gtaaaagaag ttcgtgaaaa
     3961 cgaagtgtat atgggcgaaa ttccgttgat gaccccgagc ggttcttttg tgattaacgg
     4021 cacagagcgt gtgattgttt cccagttgca ccgttcgccc ggtgtgttct tcgagcatga
     4081 caaaggtaag acacactctt ccggcaaatt gttattctcc gcccgcatca ttccctaccg
     4141 tggttcatgg ttggattttg aatttgatcc gaaagatttg ctgtatttcc gtatcgaccg
     4201 ccgccgtaaa atgccggtaa cgattttgtt gaaggctttg ggctacaaca atgagcaaat
     4261 cttggatatt ttctacgaca aagaaacgtt ttatttgtct tcaaacggtg ttcaaaccga
     4321 tttggtcgca ggccgtctga aaggcgaaac tgccaaggtc gatatcttgg ataaagaagg
     4381 caatgtattg gttgccaaag gtaagcgcat tactgcgaaa aatatccgtg atattaccaa
     4441 tgcaggcctg acccgtttgg atgtagaaca ggaaagcctg ctgggcaaag cattggctgc
     4501 cgatttgatt gattcggaaa ccggcgaggt attggcttct gccaatgatg aaattacaga
     4561 agagttgttg gccaaatttg atattaacgg cgtaaaagaa attacgaccc tttacatcaa
     4621 tgagttggat cagggtgctt atatttccaa taccttgcgc acggatgaga ctgccggccg
     4681 gcaggcggcg cgtgttgcga tttaccgtat gatgcgtccg ggcgaaccgc ccaccgaaga
     4741 ggcggtcgag caattgttta accgcttgtt cttcagtgaa gacagctacg atttgtcccg
     4801 cgtaggccgt atgaaattta atacgcgcac atacgaacaa aaactgtccg aagcccaaca
     4861 aaactcttgg tacggccgcc tgttgaacga gacgtttgcc ggtgctgccg acaaaggcgg
     4921 ttatgtcctg agcgtcgaag atattgtcgc ctcgattgcg actttggtcg agttgcgtaa
     4981 cggccatggc gaagtggacg atatcgatca cttgggcaac cgtcgagtac ggtcggtagg
     5041 cgagctgact gaaaaccaat tccgcagcgg tttggctcgt gtggaacgtg ccgtaaaaga
     5101 acgtttgaat caggcggaat cagaaaactt gatgccgcac gacttgatta atgcgaaacc
     5161 cgtttctgcc gccatcaaag aattcttcgg ctccagccaa ttgagtcagt ttatggatca
     5221 gaccaatccc ttgtctgaag taacccataa acgccgtgta tctgcattgg gcccgggcgg
     5281 tttgacccgc gaacgtgccg gcttcgaagt gcgggacgtg catccgaccc actatggacg
     5341 tgtatgtcct attgaaacgc ctgaaggtcc gaacatcggt ttgatcaact cattgtctgt
     5401 ttacgcgcgc accaatgatt acggtttctt ggaaacgcct taccgccgcg ttattgacgg
     5461 caaagtaacc gaggaaattg attacttgtc tgccatcgaa gaaggccgct atgtgattgc
     5521 acaggcgaat gccgatttgg attcagacgg caatctgatt ggcgatttgg ttacctgtcg
     5581 tgaaaaaggc gaaaccatta tggcaacgcc cgaccgcgtc caatatatgg acgtggcaac
     5641 cggtcaggtg gtatccgttg cggcatccct gattccgttc ttggaacacg atgacgcgaa
     5701 ccgcgcattg atgggtgcca acatgcaacg tcaggcagtg ccttgtctgc gtcctgaaaa
     5761 accgatggtc ggtaccggta tcgagcgttc cgttgccgtt gactctgcta ctgcaatcgt
     5821 tgcccgccga ggcggcgtgg tcgagtatgt cgatgccaac cgcgttgtga tccgtgtcca
     5881 tgacgacgaa gcgactgccg gcgaagtggg tgtcgatatt tacaatttgg ttaaattcac
     5941 ccgttccaat caatctacca acatcaacca acgtccggct gtcaaagcag gcgatgtttt
     6001 gcaacgcggc gatttggtgg ccgacggcgc gtccaccgat ttgggcgaat tggctttggg
     6061 tcaaaatatg accatcgcct tcatgccgtg gaacggttac aactacgaag actcgattct
     6121 gatttccgaa aaagtggctg cggacgaccg ctatacttcg attcacattg aggaattgaa
     6181 tgtcgttgcc cgcgatacca agctgggtgc ggaagacatt acccgcgata ttccgaactt
     6241 gtccgagcgt atgcaaaacc gtttggacga atccggtatc gtttacatcg gtgcggaagt
     6301 agaagccggc gatgtgttgg taggcaaggt aacgcctaaa ggcgaaaccc agctgacgcc
     6361 ggaagaaaaa ctgctgcgcg ccatcttcgg cgaaaaagcg tctgacgtaa aagatacttc
     6421 attgcgtatg cctaccggta tgagcggtac ggttatcgac gttcaagtct ttacccgcga
     6481 aggcatccaa cgcgacaaac gcgcccaatc cattatcgat tccgaattga aacgctaccg
     6541 tttggatttg aacgaccaat tgcgtatttt cgacaacgac gcattcgacc gtatcgagcg
     6601 tatgattgtc ggtcagaaag ccaacggcgg tccgatgaag ctggccaaag gcagcgaaat
     6661 cacgaccgaa tatctggcgg gtctgccgag caggcacgat tggtttgata tccgtttgac
     6721 cgatgaagat ttggccaagc agttggaact gattaaattg agcctgcaac aaaaacgcga
     6781 agaagcggat gagttgtatg agatcaagaa gaaaaaactg acccaaggcg acgagctgca
     6841 acccggcgta caaaaaatgg tgaaagtctt tatcgccatc aaacgccgtc tgcaagccgg
     6901 tgacaaaatg gcgggccgcc acggtaataa aggtgtggta tctcgcattc tgcctgtgga
     6961 agacatgccg tacatggcgg acggccgtcc tgtggacatc gtactgaacc cgttgggcgt
     7021 accttcccgt atgaacatcg gtcagatttt ggaagttcac ttgggttggg cggcaaaagg
     7081 tatcggcgag cgcatcgacc gtatgctgaa agagcgacgc aaagccggcg agctgcgcga
     7141 gttcttgaac aaactctaca acggcagcgg taagaaagaa gatttggaca gcctgactga
     7201 tgaagaaatc atcgaattgg cttccaatct gcgtaaaggt gcatctttcg cctctcctgt
     7261 attcgacggt gcgaaagagt ctgaaatccg cgaaatgttg aacttggctt acccaagcga
     7321 agatcctgag gttgagaaac tgggcttcaa cgacagtaaa actcaaatca cgctgtatga
     7381 cggccgttca ggcgaagcat ttgaccgcaa ggttacagtc ggtgtgatgc actatctgaa
     7441 actgcaccac ttggttgacg aaaaaatgca cgcgcgttct accggcccgt acagtctggt
     7501 tactcaacaa cctctgggcg gtaaagctca gtttggcggc caacgtttcg gtgagatgga
     7561 ggtttgggca ttggaagcat acggcgcggc atacacgctg caagagatgc tgactgtgaa
     7621 gtctgacgac gtgaacggcc gtaccaaaat gtacgaaaac atcgtcaaag gcgaacacaa
     7681 aatcgatgcc ggtatgcccg agtccttcaa cgtattggtc aaagagattc gctcactggg
     7741 tttggatatc gatttggaac gctactgata cgggtttcag acggcataag gggagctgtt
     7801 ctgcaggtat gcggggcagc ccgacaatgt ttaaaaacga aatgccgtct gaaagcactg
     7861 tacctttatc catatcgaaa atccgccacg cggtaaaaat atttccttca aggagcagaa
     7921 atgaatttgt tgaacttatt taatccgttg caaaccgcag gtatggaaga agagtttgat
     7981 gccatcaaaa tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtt
     8041 aaaaaacctg aaaccatcaa ctaccgtacg ttcaaacccg agcgcgacgg cttgttctgc
     8101 gccaaaatct ttggcccggt caaagactac gaatgcttgt gcggaaaata caaacgattg
     8161 aaatttaaag gcgtaacctg tgaaaaatgt ggcgtagaag ttaccctgtc caaagtgcgc
     8221 cgcgaacgca tgggccatat cgaattggct gcacccgttg cccatatttg gttcttgaaa
     8281 tccctgcctt cccgcttggg tatggtattg aacatgacgt tgcgtgatat cgaacgcgta
     8341 ttgtactttg aagcattcgt ggtgaccgat cccggcatga ctccgttgca acgtcgtcaa
     8401 ttgctgactg aagacgatta ctacaacaaa ctggacgaat acggcgacga cttcgatgcc
     8461 aaaatgggtg cggaaggtat ccgcgaattg ttgcgaacct tggatgtagc aggcgaaatc
     8521 gaaatcctgc gccaagagct tgaatcgacc ggttctgaca ccaaaatcaa aaaaatcgct
     8581 aaacgcttga aagtattgga agccttccat cgttccggta tgaagctgga gtggatgatt
     8641 atggatgtgc tgccggtatt gccgcctgat ttgcgtccgt tggttccgtt ggacggcggc
     8701 cgttttgcca cttccgattt gaacgatttg taccgccgcg tcatcaaccg caacaaccgt
     8761 ctgaaacgcc tgctggaact gcatgcgcct gacatcatcg tccgcaacga aaaacgtatg
     8821 ttgcaagaag cggttgattc gctgttggat aacggccgtc gcggtaaagc catgaccgga
     8881 gccaacaaac gtccgctgaa atcgttggca gatatgatta aaggtaaagg cggccgcttc
     8941 cgtcaaaacc tgttgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc
     9001 ccatacctgc gtctgcacca atgcggtttg ccgaaaaaaa tggcgttgga actgttcaaa
     9061 ccatttattt tccacaaatt ggaaaaacaa ggtttggcgt ctaccgttaa agctgcgaaa
     9121 aaattggtag aacaagaagt acctgaagta tgggatatct tggaagaagt catccgcgaa
     9181 catccgatta tgttgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgag
     9241 cctattctga tcgaaggtaa agcgattcag ttgcacccat tggtgtgtgc cgcatttaat
     9301 gccgactttg acggtgacca aatggcagta cacgttccat tgagcttgga agcgcaaatg
     9361 gaagcacgca cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg
     9421 attatcgtac cttcccaaga catcgtattg ggtctgtact acatgacccg cgaccgtatc
     9481 aatgccaaag gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg tgcataccat
     9541 accaaacagg tcgagctggg tacaaaaata accgtacgtc tgcgcgaatg ggtgaaaaac
     9601 gaagcaggtg agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg
     9661 ttgagcgaaa tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa
     9721 aaagaaattt ccaagctgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt
     9781 atcttcgccg accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc
     9841 attgccgttg acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat
     9901 gccgaagtta aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc
     9961 tacaacaaag tggtggatat ttggggtcgt gccggcgata agattgctaa agcgatgatg
    10021 gacaacctgt ccaaacaaaa agttatcgac cgtgacggca acgaagtcga tcaagagtca
    10081 ttcaactcca tttatatgat ggcggactcc ggtgcccgtg gttctgcggc tcagattaaa
    10141 cagttgtccg gtatgcgcgg tttgatggcg aaacctgacg gatcgattat tgaaacgccg
    10201 attacctcaa acttccgcga aggtctgacc gtattgcaat actttattgc gacccacggt
    10261 gcgcgtaagg gtttggcgga taccgcgttg aaaaccgcga actccggtta cctgacccgt
    10321 cgtctggtag acgtaactca agacttggtc gttgttgaag acgattgcgg tacttcagac
    10381 ggctttgtca tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt
    10441 attttgggtc gtgttaccgc gtctgacgtt gtcgatccgt caagcggcga gactttggtt
    10501 gaagccggta cgttgttgac tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat
    10561 gaagtcaaag tccgtactcc gattacttgt aaaacccgcc acggtctgtg cgcgcactgt
    10621 tacggccgcg acttggcgcg cggcaaactg gtcaatgccg gcgaggcagt cggcgtgatt
    10681 gcggctcagt ctattggtga accgggtacc cagttgacca tgcgtacgtt ccacatcggt
    10741 ggtgcggcat cccgtgcggc agcagccagc caagtcgaag ccaaatccaa cggtacggca
    10801 cgtttcagca gccaaatgcg ttatgttgcc aacaacaaag gcgagttggt tgtcatcggc
    10861 cgttcttgcg aagtcgtgat tcacgatgac atcggccgtg aacgcgaacg ccacaaagta
    10921 ccttacggtg ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg
    10981 gcaacttggg atccgcatac ccgtccgatg attaccgaac atgcaggtat ggtgaaattc
    11041 gagaacatgg aagagggcgt taccgttgca aaacaaaccg atgatgtaac cggtttgtcc
    11101 gctttggtag tgattgacgg taaacgccgt tcctctagtg cttccaaact gctgcgtccg
    11161 actgtgaaac tcttggacga aaacggcgtg gaaatctgta ttcccggtac gtccactccg
    11221 gtttcgatgg cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt
    11281 aaaggcgacg tattggcgcg tattccgcaa gcctcttcca aaacccgcga cattaccggc
    11341 ggtctgccgc gcgttgccga gctgtttgaa gcacgcgtgc cgaaagatgc cggtatgctg
    11401 gcggaaatta ccggtaccgt ttccttcggt aaggagacca aaggcaaaca acgtctgatt
    11461 atcactgacg tggacggtgt agcatacgag actttgattt ccaaagagaa acaaattctg
    11521 gtacatgacg gtcaagtggt aaaccgaggc gaaaccattg tggacggcgc ggtcgatccg
    11581 cacgatattc tgcgtctgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg
    11641 caagaggttt accgcctgca aggtgtgaag atttccgata aacacatcga agtcatcatc
    11701 cgtcaaatgt tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt tattaccgga
    11761 gagcaggtcg aacgcggcga tatgatggcg gccaatgaaa aagctttgga agaaggcaaa
    11821 gagccggcac gttacgaaaa catattgctg ggtattacca aagcctccct gtccaccgac
    11881 agcttcattt ctgccgcatc gttccaagaa accacccgtg tgttgaccga agctgccatc
    11941 atgggcaaac aagacgaact gcgcggtctg aaagagaatg tcatcgtcgg tcgcctgatt
    12001 cctgccggta ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag
    12061 caggagactg ctgaaaccca agtaacggat gaataatctt cggcgcgttc attcaataaa
    12121 aaaccgcaag ccttgagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt
    12181 tcgtcatttt gaggcgtgtg gatataagcc cgataagtgt cccatatctt cgtgccgtca
    12241 aattatgcat cgaatagaac gagttaaatc cgaaggttat tgtgttaaat attgcaaaaa
    12301 tactttaaaa atttcgcacg gtgggagggt agaattccca gacttaaagc ttaagttttt
    12361 ttaacaaagt ttgggggagt ctaaatgaaa gtatctcaat cagcaatgaa taaacttgtt
    12421 attgctgtta ttgcttctat ggggctaaca gcgtgcgcgg gtggcggttc cgattcatct
    12481 atgtcggttc aaccaagcgt ttcggaacaa ctgaaagaca atgcaaatgt tgatgcgaaa
    12541 gatgaaaaag ttattgaata tctgaagaaa tcatcattaa aagatgtacc aaaagaattg
    12601 caagccaaag tattaaaggt taaaggtgat gaatatacag gagtgcgaaa acaatatgca
    12661 ggtaagctcg gtaagggtga atctgtcaag gcaatgctat ttctagacgg cgaagagccg
    12721 tttagtaagg aacaacttca gaagatggat gtatatgtta atggtaaaaa atacgaaggt
    12781 agtaaggggg ggaactagat gttctcccca aaggtttgag tgagcaaaag attgagtttt
    12841 acggtgcgga taaagaacag aattatgcgc tattaaaaac ttgggtttat gagcaaccgt
    12901 attctgtggt tagaggctat tttggctaca gtcgaaaaga cggtaatcct atagaagagg
    12961 atggacaaaa tcctgaagag attccttttg acttgtatct gggcgacatt agaggcgtgg
    13021 caacagatga agacaaatta cccaaggctg ggagctttca atatgaaggt cgggcatttg
    13081 gcggtaatgg ggttttatcc aaagaatcgt tagataacca taatggcgta tttagataca
    13141 caattgattt tgatcgtaga aaaggctctg gctcaattga aggaatggaa caatatggca
    13201 agatcaaatt agaagaagct gctattgaga gaatccctta tcgagagtcc ggctccagct
    13261 tggggttgaa agaccgggtt tcttatttcg gcgtgaacga aggggtggct atgttagaga
    13321 aggataatga gattaaaaag tatcatttgg gtattttcgg cgaagcggct aatgaagtag
    13381 caggtgctgt aagccaagag cataaacatc aagctgttat cggtttcggc ggcgagaaga
    13441 aatagagaaa gcactatttc tatttagtgc cgtttgtccg ggcaagatgc cgtctgaagt
    13501 ttcagacggc attttcttca aaaaacagtc cgccaaatcg aatactgcca agcggcaatg
    13561 caatacctca aaacagcaat aatttctttt aaaacagttg attcttactg aacataaggc
    13621 cgggcaatac gaaaagaaaa atggcgttgc tgggcatttt atttgaattt gttatgaaat
    13681 tgatgaaatc taaaaatatc ttttttatgg gatacctaat cgcttcggcg acgtttgccg
    13741 aggatatcgg cgtgcctgtc gaaccgatta acgtcggtag tcgggctgcg atgccgtctg
    13801 aaggggaaag cctcgccctc ctgccgtttg cagaggatgt gccgccggtt cgcgatgcaa
    13861 tgccgtctga agttcctaaa agcgcggcag gcggcgatgt tcgaggcggc cgatctatac
    13921 cgtgagttgt tgtcggaaag acccgatctt gtttatcccc gttttgattt gggcgtgatg
    13981 ctgtttgaag acaaacaata tcgtgaggca ttggttcagt tgcaccgggc cgaggaggtg
    14041 ttgccgcccg atatgcggca gcttgcacgg gaatatatcc gtcaggcgga agcggtgcag
    14101 gcgtggcatc cttcgttcaa tatgaactat gaacaaacag ataatgtaaa caatgcgtcc
    14161 ctttcacggg acatcgtcat caacggccgg aaatggataa aatcggaaga cagcctgccc
    14221 aaacgcgcca acgggataag gtatgaattg ggtattgacc ggatgtttaa catggcaggc
    14281 aatcactttg cccgcttagg catcagcggt tccggtgtgc attattggaa tgcccgggat
    14341 tttagcgaac aggcgtttca tgccgaagta ggctaccgtt atcggaatag ccgtctagag
    14401 tggggattcc ggccctttgt caaacaaaac cggctgggca ataaccgtta tacggcaaat
    14461 acgggtattg cgttggatta cagccgccgt ctgaatgaaa aatggcgcag cacccaatct
    14521 ttccagtatg gccgcaaaca ataccatgac gaatatttgg caaaacgtta caacagtaaa
    14581 acgatttcgg tgagcggtac gttcagctat tacgctatgt cggcttggca gttgtacggg
    14641 ggtattagcg gtatgtttga taatactgtg gagaaagagc aggcttcacg gcgttatggt
    14701 gtaagtttgg gtacggtgaa aatattagac ggcggtcttg gactgaagtt gggtgcgggt
    14761 tacacgaaac ggatatttaa agcccctgca acattaattt acaattttac tcggcgtgat
    14821 gattaacatc ggatgagcgc ggctttatgg cataaaaaac tgtcgtggaa aggatttaca
    14881 ccccaaataa atttccgtta caacaagaac aacagcaata tgcccgcctt ttattcgcac
    14941 agcggcaagg gatggtttgt cagtatggaa aaaacgtatt gacagtattt tctccagccg
    15001 tccgactgat tgtgagggat gtcggtaaat atttatcggc aaacaagaaa atcatctttt
    15061 ttcttgtcgt tatgcttgac tgtctgcttg caataaaaat ataattccac tcttgccgac
    15121 atggtgtcgg caagtattta actcaacagg acgagaaaat atgccaacta tcaaccaatt
    15181 ggtacgcaaa ggccgtcaaa agcccgtgta cgtaaacaaa gtgcccgcac tggaagcctg
    15241 cccgcaaaaa cgcggcgtgt gcacccgtgt atacacgact acccctaaaa aacctaactc
    15301 tgcattgcgt aaagtatgta aagtccgcct gaccaacggt tttgaagtca tttcatatat
    15361 cggcggtgaa ggccacaacc tgcaagagca cagcgtcgta ctgattcgcg gcggccgtgt
    15421 aaaagacttg ccgggtgtac gttaccacac tgtacgcggt tctttggata ctgcaggtgt
    15481 taaagaccgc aaacaagccc gttctaaata cggtgctaag cgtcctaaat aattaccggg
    15541 actcaaatag gcacgtcggc cgcctaagct gaacaacggc cgagtaagtg aatactcaat
    15601 tgggtattca tgggaataga cccgactgaa tagattaaag gaaattaaaa tgccaagacg
    15661 tagagaagtc cccaagcgcg acgtactgcc agatcctaaa ttcggtagcg tcgagttgac
    15721 caaattcatg aacgtattga tgattgacgg taaaaaatcc gttgccgagc gtatcgttta
    15781 cggtgcgttg gaacagattg agaaaaaaac cggcaaagca gcaatcgaag tatttaacga
    15841 agccattgca aactccaaac ctatcgtgga agtgaaaagc cgccgtgtag gtggtgcaaa
    15901 ctaccaagtt cctgttgaag ttcgtccttc acgccgtctg gctttggcaa tgcgttgggt
    15961 tcgcgacgcg gcccgcaaac gtggtgagaa atccatggat ctgcgtttgg caggcgagtt
    16021 gattgatgcg tccgaaggcc gtggcggtgc gttgaaaaaa cgtgaagaag tacaccgtat
    16081 ggctgaagcc aacaaagcat tctctcactt ccgtttctaa ttttgaaagg ctaataaaat
    16141 ggctcgtaag accccgatca gcctgtaccg caacatcggt atttccgccc atatcgatgc
    16201 gggtaaaacc acgacgacag aacgtatttt gttctatacc ggtttgaccc acaagctggg
    16261 cgaagtgcat gacggtgcgg ctactaccga ctacatggaa caagagcaag agcgcggtat
    16321 taccattacc tccgctgccg ttacttccta ctggtccggt atggcgaaac aattccccga
    16381 gcaccgcttc aacatcatcg acaccccggg gcacgttgac tttaccgtag aggtagagcg
    16441 ttctatgcgt gtattggacg gcgcggtaat ggtttactgt gcggtgggcg gtgttcaacc
    16501 gcaatctgaa accgtatggc ggcaagccaa caaataccaa gttccgcgct tggcgtttgt
    16561 caataaaatg gaccgccaag gtgccaactt cttccgcgtt gtcgagcaaa tgaaaacccg
    16621 tttgcgcgca aaccccgtac ctatcgtcat tccggtaggc gcggaagaca gtttcaccgg
    16681 tgttgtcgat ttgctgaaaa tgaaatctat catctggaat gaagccgata aaggtacaac
    16741 ctttacctat ggcgatattc ctgccgaatt ggtcgaaact gccgaagaat ggcgtcaaaa
    16801 tatgattgaa gccgcagccg aagccagcga agaactgatg gacaaatact tgggcggtga
    16861 agatctggcc gaagaagaaa tcgtaggcgc gttgcgtcaa cgtactttgg caggcgaaat
    16921 tcagcctatg ctgtgcggtt ctgcatttaa aaacaaaggt gttcaacgta tgttggacgc
    16981 agttgtagaa ttgctgccag ctcctaccga tattcctccg gttcaaggtg ttaatcctaa
    17041 cactgaagaa gccgacagcc gtcaagccag cgatgaagag aaattctctg cattggcatt
    17101 caaaatgttg aacgacaaat acgtcggtca gctgaccttt atccgcgttt actcaggcgt
    17161 agtaaaatcc ggcgataccg tactgaattc tgtaaaaggc actcgcgaac gtatcggtcg
    17221 tttggtgcaa atgactgccg cagaccgtac tgaaatcgaa gaagtacgcg ctggcgacat
    17281 cgcagccgct atcggtctga aagacgttac taccggtgaa accttgtgtg cggaaagcgc
    17341 gccgattatc ttggaacgta tggaattccc cgagccggta atccatattg ccgttgagcc
    17401 gaaaaccaaa gccgaccaag agaaaatggg tatcgccctg aaccgcttgg ctaaagaaga
    17461 cccttctttc cgcgttcgta cagacgaaga atccggtcaa accattattt ccggtatggg
    17521 tgagctgcac ttggaaatta ttgttgaccg tatgaaacgc gaattcggtg tggaagcaaa
    17581 tatcggtgca cctcaagtgg cttaccgtga aactatccgc aaagccgtta aagctgaata
    17641 caaacatgca aaacaatccg gtggtaaagg tcaatacggt cacgttgtga ttgaaatgga
    17701 acctatggaa ccgggtggtg aaggttacga gtttatcgat gaaattaaag gtggtgtgat
    17761 tcctcgcgaa tttattccgt ctgtcgataa aggtatccgc gatacgttgc ctaacggtat
    17821 cgttgccggc tatcctgtag ttgacgtacg tatccgtctg gtattcggtt cttaccatga
    17881 tgtcgactct tcccaattgg catttgaatt ggctgcttct caagcgttta aagaaggtat
    17941 gcgtcaagca tctcctgccc tgcttgagcc gattatggca gttgaagtgg aaactccgga
    18001 agaatacatg ggcgacgtaa tgggcgactt gaaccgccgt cgcggtgttg tattgggtat
    18061 ggatgatgac ggtatcggcg gtaaaaaagt ccgtgccgaa gtacctctgg cagaaatgtt
    18121 cggttactcg accgacctgc gttctgcaac ccaaggccgc gctacttact ctatggagtt
    18181 caagaaatat tctgaagctc ctgcccacat agctgctgct gtaactgaag cccgtaaagg
    18241 ctaatcaggc aaataggccg tctgaaaggc tgaaatgatt tttcagatgg cattgttctt
    18301 taatcgatct ttaatgtaaa ggaattagct catggctaag gaaaaattcg aacgtagcaa
    18361 accgcacgta aacgttggca ccatcggtca cgttgaccat ggtaaaacca ccctgactgc
    18421 cgctttgact actattttag ctaaaaaatt cggcggcgct gcaaaagctt acgaccaaat
    18481 cgacaacgca cccgaagaaa aagcacgcgg tattaccatt aacacctcgc acgtagaata
    18541 cgaaaccgaa acccgccact acgcacacgt agactgtccg ggtcacgccg actacgttaa
    18601 aaacatgatt accggcgccg cacaaatgga cggtgcaatc ctggtatgtt ctgctgccga
    18661 cggccctatg ccgcaaaccc gcgaacacat cctgctggcc cgtcaagtag gcgtacctta
    18721 catcatcgtg ttcatgaaca aatgcgacat ggtcgacgat gccgagctgt tggaactggt
    18781 tgaaatggaa atccgcgacc tgctgtccag ctacgacttc cccggcgacg actgcccgat
    18841 cgtacaaggt tccgcactga aagccttgga aggcgatgcc gcttacgaag aaaaaatctt
    18901 cgaactggct accgcattgg acagctacat cccgactccc gagcgtgccg tggacaaacc
    18961 attcctgctg cctatcgaag acgtgttctc catttccggc cgcggtaccg tagtcaccgg
    19021 ccgtgtagag cgaggtatca tccacgttgg tgacgagatt gaaatcgtcg gtctgaaaga
    19081 aacccaaaaa accacctgta ccggcgttga aatgttccgc aaactgctgg acgaaggtca
    19141 ggcgggcgac aacgtaggcg tattgctgcg cggtaccaaa cgtgaagacg tagaacgcgg
    19201 tcaggtattg gccaaaccgg gtactatcac tcctcacacc aagttcaaag cagaagtgta
    19261 cgtattgagc aaagaagagg gcggccgcca taccccgttt ttcgccaact accgtcccca
    19321 attctacttc cgtaccactg acgtaaccgg cgcggttact ttggaaaaag gtgtggaaat
    19381 ggtaatgccg ggtgagaacg taaccattac tgtagaactg attgcgccta tcgctatgga
    19441 agaaggtctg cgctttgcga ttcgcgaagg cggccgtacc gtgggtgccg gcgtg
//
LOCUS       DAWTHD010000025        19409 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWTHD010000025 DAWTHD010000000
VERSION     DAWTHD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19409
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.025"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..947)
                     /gene="mafB"
                     /locus_tag="WM704_000728"
     CDS             complement(<1..947)
                     /gene="mafB"
                     /locus_tag="WM704_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFA9340198.1"
                     /translation="MKPLRRLIKLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRRGNSMEFVNGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSVAGVEKNTREAVDRWIQENPNAAETVEAVFNV"
     gene            complement(951..1892)
                     /gene="mafA"
                     /locus_tag="WM704_000729"
     CDS             complement(951..1892)
                     /gene="mafA"
                     /locus_tag="WM704_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFA9340199.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSATRYSYPA
                     YDTTATTKSDALSGVTTSTSVLNAPAAALTKNNGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            complement(2011..3111)
                     /locus_tag="WM704_000730"
     CDS             complement(2011..3111)
                     /locus_tag="WM704_000730"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFA9340200.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            3436..5010
                     /locus_tag="WM704_000731"
     CDS             3436..5010
                     /locus_tag="WM704_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFA9340201.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            5024..5212
                     /locus_tag="WM704_000732"
     CDS             5024..5212
                     /locus_tag="WM704_000732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340202.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(5250..6671)
                     /gene="hemN"
                     /locus_tag="WM704_000733"
     CDS             complement(5250..6671)
                     /gene="hemN"
                     /locus_tag="WM704_000733"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFA9340203.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIK
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPGDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            6806..7540
                     /gene="fnr"
                     /locus_tag="WM704_000734"
     CDS             6806..7540
                     /gene="fnr"
                     /locus_tag="WM704_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFA9340204.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(7653..8603)
                     /locus_tag="WM704_000735"
     CDS             complement(7653..8603)
                     /locus_tag="WM704_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFA9340205.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            8967..9677
                     /gene="rmpM"
                     /locus_tag="WM704_000736"
     CDS             8967..9677
                     /gene="rmpM"
                     /locus_tag="WM704_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFA9340206.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            10452..11408
                     /gene="thiL"
                     /locus_tag="WM704_000737"
     CDS             10452..11408
                     /gene="thiL"
                     /locus_tag="WM704_000737"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFA9340207.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            11401..11886
                     /locus_tag="WM704_000738"
     CDS             11401..11886
                     /locus_tag="WM704_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFA9340208.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(11946..13622)
                     /gene="ettA"
                     /locus_tag="WM704_000739"
     CDS             complement(11946..13622)
                     /gene="ettA"
                     /locus_tag="WM704_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFA9340209.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGDIKIGYLPQEPELDPEKTVREEVESGFGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            13776..14795
                     /locus_tag="WM704_000740"
     CDS             13776..14795
                     /locus_tag="WM704_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9340210.1"
                     /translation="MIGDRRAGNHFGFSKANTFQIKKKDLLYVGIYASNSKTRFARAG
                     KKHDLDVELRLSRRSDGLYPAKLADEPHFSNARRRPAQFGLVFHPAAAGGDAGSADSG
                     YYSDRTWKPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIAL
                     LDVSSNMAMQPFKMMVGDMVNEEQKSYAYGIQSFLANTDAVVAAILPFVFAYIGLANT
                     AEKGVVPQTVVVAFYVGAALLIITSAFTISKVKEYDPETYARYHGIDVAANQEKANWF
                     ELLKTAPKVFWTVTPVQFFCWFAFRYMWTYSAGAIAENVWHTTDASSVGHQEAGNRYG
                     VLAAV"
     gene            14808..15212
                     /locus_tag="WM704_000741"
     CDS             14808..15212
                     /locus_tag="WM704_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFA9340211.1"
                     /translation="MICSFILAKVPNKYHKAGYFGCLALGALGFFSIFFIYNQYALIL
                     SYILIGIAWAGIITYPLTIVANALSGKHMDTYLGLFNGSVCMPQIVASLLSFVLFPML
                     GGHQATMFLVAGAVLLLGAFSVCLIKETHGGV"
     gene            15216..16177
                     /locus_tag="WM704_000742"
                     /pseudo
     CDS             15216..16177
                     /locus_tag="WM704_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            16424..18681
                     /locus_tag="WM704_000743"
                     /pseudo
     CDS             16424..18681
                     /locus_tag="WM704_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            18694..19344
                     /gene="pgmB"
                     /locus_tag="WM704_000744"
     CDS             18694..19344
                     /gene="pgmB"
                     /locus_tag="WM704_000744"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFA9340212.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYLAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGETEFAELTCRKNDNYLEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPSPDIFLAAAEGVGA
                     DIRRCRRRRHQSLWRFAHRRGQNRRLGQRHRAGVRHRRADLRLLAKCVGTVGQIKF"
BASE COUNT         4065 a         4997 c         5571 g         4776 t
ORIGIN      
        1 acgttgaaga cggcttcgac cgtttcggcg gcattggggt tttcctgtat ccaccggtca
       61 acggcttcgc gcgtattctt ttcaacgccc gccacgctgc ccaagccgcc gatgacggcg
      121 aatttgccct cggcgggcaa gggggcgatg ttgcgcatcg cggctttgtc tatggcatag
      181 cgcgttccgt acagtatgtc gcctatgccc aaggcttcgc ccgcgctgat aaaggggttg
      241 agcgcgccgg cggcgacgcc gttgacaaac tccatgctgt tgccccggcg gtcgagcttg
      301 gcattgtgct cgaacatttt tctgttggct tcatcggcgc ggtcggagaa attgctgccg
      361 aggttgttgt agttgtcgga tatgcgttgc cggatgctgc gggtgtcggt cggattgagt
      421 ttgatactgc gggctgtgcc gttgacgtga taggtgtatt cgtctcgtgc gcccgtaggt
      481 ttggggtaat tgccgccctt cgggccgtcg taggcatcgg cgggatgatg ttcgtgtcct
      541 tcccagttga gccggtacac ggtaaagccg tcgtcaacgt tgcctttttc ttcgcttgcg
      601 ctgtcggcgg cgtggttgtc gaagggggcg tgttcttcgt gtccgtgtcc ggaaaagcgg
      661 acggtgtaac caagattgcc ttggattgcc gcctgttgga tgagcaggtt gcccatctgg
      721 tgggtatagt cttggatgac gttgattttg ccggtgcggt cggaaacgct gccgcgcggg
      781 tcgccgaaga ggtggtattt gccgccgggt tcgtagtgct gccgttgggt gttatcggta
      841 atgaacgggt cttgcgccaa gtccgccgcg agggcgggct gtatgagtgc ggccgccgct
      901 acggcacagg cggcaaggag cttgatgagt cttcgcagcg gtttcacggt ttatcctcct
      961 ttgcggcggc ggatgacttc gttgccgaca tcggggtttt taccgttgtt ttgtttgaag
     1021 tcgggacggt tttgggcggt tgtgtcgccg tagggggtga tgtcggagaa atcgaccatc
     1081 aggcggtctg aggctttgac ggttttgccg acgctgtaag gtcccatcca gagggcgtat
     1141 tgttcttggt attgggattc gtaggcggcg gttttagggg caatcagcag tttccggctg
     1201 tcgcggtcga cggcgaaata ttcgagcttg gtttgggctt taagggtttc ggcgttgtag
     1261 aggtgcagtt cggtacggct gcggacggtg ccgaatacgt cgacggttac gaatacgtcg
     1321 gtgtcggcgt attcgggcgg tacgacttcg atgccgcgca ggtagaagac ggtttggatg
     1381 aggttggtca ggaaggaaac gtcgcggggg ttggcgagca gggtttcgtt gcggtagtcg
     1441 cccgtgccgt tgacggacag tccggcggag cgttcgcctt tgcgtccgtt gtttttcgtc
     1501 agggcggcgg cgggggcgtt taagaccgat gtggaagtgg ttacgccgga gagcgcgtcg
     1561 gatttggtgg tggcggtagt gtcataggcg gggtagctgt atcgggtggc gctgtcgggg
     1621 ttgttgtggt agccgccgcg tatcagtgcg tcgatggagt agcgtccgcc gcttatgttg
     1681 cccgaacctt ggtcgcccat aacggagacg taaagggcgg ctttgcgtcc tttcagggcg
     1741 gacaagtcca tttctttgac ggcggcgcgg gacgatgcgg cgacgagttc ctgttcgacg
     1801 gcaaagcgtt tgccgccgcc gtgggcgggt atgccggtca gtgtgccgca ggcggtgagt
     1861 acgaggggga tgaggaggag cagggttttc atggcagggt ttgttttgtt ttgaacgggt
     1921 ttgagtgtaa atggatttta agggtttgta aacgaaaggg gtggaaatgc cgtctgaacg
     1981 gcggaaatgg ctttcagacg gtatttgcgc tcaataataa tatcccgcgc ccagaataca
     2041 cggtttggat gcgccggtcg ctttgtgcgg actaccggga atgcggttaa tccaacacgc
     2101 cgccaaccac gcaaatgcgg ccgcctccac ccattgagga tcgaggttca gttcggcggt
     2161 gctgtgcagg gaaacgcgtg tgccgaaaca ttctgccaaa tccgccatta aaacaggatt
     2221 gcggatgccg ccgccgcaaa tgtacatttg acgggcatct gccgctgcgt gtgagacggc
     2281 gtcgcaaacg gtttgcgcgg tgaatcggga aagcgtccgc aatacgtcgt atcggttttc
     2341 gccgccgtca aggtaggttt cgagccaatt tagggcaaac agttcgcgcc ccgtgctttt
     2401 tgggtggggt tgtgagaaat acgggtgggc gagcagcctg ccgagcagtt gcggcaatat
     2461 gttgccttgt gccgcctttg caccgttttt gtcgtaaggc agctgccagt gtgcctgcgt
     2521 ccacgcgtcc atcagcatat tgcccggccc tgtgtcgaag ccgaaggcgg gtgcgccggg
     2581 ggggagtacg ctgatgttgg caatcccgcc gatgttcagt accacgcgtg tttccctgtc
     2641 atcgcggaac agggcttcgt gaaaggcggg gacgagcggc gcaccttgtc cgccggcagc
     2701 aaggtcgcgg ctgcggaagt cgccgacggt aaaaatccgc gtcagttccg ccagcagcgg
     2761 caaatcggca agctgtatgc tgtaaccgtg ttccggcgcg tgtcggacgg tttgcccgtg
     2821 gcagccgagg gcggtaatgt cgcacggagc gaggttttga ctgcacagca gttcggcggc
     2881 ggtttgcgcg tacaggcggc tgagttcttg cgacaacatc ctgctgcggt gcagttcgtc
     2941 tgtgcctgtg tcctgcaaat ccagcaattt gcggcgcaac cggtcagggt agggggtaaa
     3001 ggcgtgccct tccgcgccca gccatttgcc gccgtccatc cgtaccagca cggcatccgc
     3061 cccgtccata ctggttcccg acataatgcc gatgtaaagc tgtgtttcca tcatcactcc
     3121 caaactggtg caaaacgcca ttttaacgtg tattgacgct cgtataccga tttgccgccg
     3181 cagtgtaaat aaagtgtaaa taaatgtttt aagacgggtg gaaaaatatt ataatgcgcc
     3241 cgcaacatcc agtagtagaa gtgtcataca aaccgtttcc ggcagcagtt ttgcattcgg
     3301 tcaggtttgg gggtattcgg atgcggttca gaaggaagga tgcgtctgcc atatcccgaa
     3361 acggcagttc gaccggaggc agcaatacag tgtcggcaac actcatgatt tccaccacat
     3421 taaaggaaga ttgccatggc tcaaatccaa atgagcgcaa atgttaaaac catcaacgcc
     3481 gtctttgccg ccatgctggt aggtacagtc ggctatttta tttattgggg cttgggttat
     3541 acccattaca attacgccgc cttattcatt attgccacga tgttcggtgt gtttatggcg
     3601 ttcaacatcg gcggcaacga tgttgccaat tctttcggca ccagcgtcgg tgcaggcacg
     3661 ctgaccatcc cgcaggcttt gctgattgcg gcggtatttg aggtcagcgg cgcggtcatc
     3721 gcgggcggcg aggtaaccga taccatacgc aaaggcatcg tcgatttgaa gggtgttgat
     3781 ttcgaaccca tacagtttgt gtttattatg atgtccgcgc ttttggcggc ggcgttgtgg
     3841 ctgctgttcg cgtcccgcaa aggcctgccg gtttcgacga cgcacgcgat tatcggcggc
     3901 atcgtcggca gtgcgttgtg tatggcgttt acgcataatg cggacggggt ggcgttgata
     3961 cggtggggca agctgggcga aatcgggatg tcttgggtat tgtcgcccgt gttgggcggc
     4021 gcggtgtcct atttcctgtt ttcgcgcgtc aagaaaaacg tcttagatta caacgcttgg
     4081 gcggaaggaa cgctcaaggg catcaagcag gaaaaaaagg cctataaaga acggcaccgc
     4141 ctgtttttcg agggtttgtc cgaagccgaa aaagtcgagt acgccaccaa aatggcgcac
     4201 gacgcgcaaa tttacgacga acccgaattc gatcctcaag agctgcaatc ggagtattac
     4261 cgcggtcttt atgcgttcga caaccgtaaa aacaatgtcg attcctacaa ggcgctgcat
     4321 tcttggattc cctttatcgc ttcgttcggc acgatgatga tttccgctat gctgattttc
     4381 aagggcttga aaaacctgca tttggggatg agcaacgtca acagcttcct gaccatcttt
     4441 atgataggcg cggcggtgtg gatggggacg tttgtgtttg ccaaaagcct caagcgtaaa
     4501 gacttgggca aatcgacctt tcagatgttt tcatggatgc aggtctttac cgcctgcggc
     4561 ttcgcattca gccacggtgc gaacgatatc gccaacgcca tcggcccgtt tgccgcgatt
     4621 atggatgttt tgcggacgaa cagcgttgcc gcgcaaagcg ccgtccctcc gattgcgatg
     4681 ctgactttcg gcatcgcgct gattgtcggt ttgtggtttg tcggtaaaga ggtgattaaa
     4741 accgtcggta cgagtttggc ggaaatgcat cctgcttcgg gttttaccgc cgaactgtcc
     4801 gccgcatccg tcgtgatggg cgcgtcgctg atggggctgc ccgtgtccag tacgcatatc
     4861 ttggtcggcg cggtactcgg tatcggtctg gtcaaccgca atgccaactg gaaactgatg
     4921 aaacccatcg gtttggcgtg ggtcattacc ctgcctgccg ccgctgtatt gtcgatggcc
     4981 agctatttga ttttgcaggc agtgttctga ttgtaaaata ctgatgccgt ctgaacccgt
     5041 gttcagacgg cattttgttg atggaatgtg cgggcttgtg ccttatgcac aatctgttct
     5101 gtcgggatat gctgtttggt atcttggttt cggaacggtc ggacacaaag gttatgatat
     5161 gccgccgcct gttcttgaaa acacttatcc tcggcagcaa aatgccgtct gaaaaagcct
     5221 ttcagacggc ttttgtacgt tagccgcaat cacaccgttt gcgaatattt cgctttggtt
     5281 tctttgtggc gcaggtggta gtcgaagacc atggcgatgt tgcggatgag gaagcgtcct
     5341 ttcggtgtaa cggtcagccc gtggctgttc aggcgcacca atcccaaacc ggcgagtttt
     5401 tccaaatccg ccagttcgtc tttgaagtag cggtcgaacg ggatgccgaa catactttcg
     5461 taaatcctgt agtcgagcgc gaagcggcac attaaatcct gaatgatgtt gcggcgcagg
     5521 atgtcgtcct gattgagctg gtagccgcgc atgatgggca gtctgccttc gtcgatggcg
     5581 gcatagtagg catcgatgtc gcgttcgttt tgggaatagg tgctgccgat tttgccgatg
     5641 gacgacacgc cgatggcgac caaatcgcaa tccgcgtagg tcgaatagcc ttggaagttg
     5701 cgttggagga agccttcttt gagggcgatg gagagttcgt cgccaggttt ggcgaaatgg
     5761 tccatgccga tgaagacgta gccgcgctct gtcagggttt ggacgcagta ttgcagcata
     5821 tcgagcttct cttcgctgtc ggggacggcg gcggtatcga tgcggcgttg cggtttgaac
     5881 acgtgcggca ggtgggcgta gtggtaaagg gcgaggcggt cgggatcgag cgacaaaacg
     5941 gtatctatgg tggttttgat gctttcggaa gtctgatgcg gcaagccgta aatcaaatcg
     6001 acgctgacgg atttgaaccc tgcctcacgg gcggcatcga tgacttcttt ggtttcttcg
     6061 tagctttgga tgcggttgac tgccgcctgc actttggggt cgaaatcctg aatgccgacg
     6121 ctcatgcggt tgaagccgag tctgccgagc atgaggacgg tgtcgcggct gactttgcgc
     6181 gggtcgattt cgatggaata ttcgccggac ggtatcagtt cgaaatgttt gcggatcatg
     6241 cggaagacac gttcgatctg ttcgtcgctc aaaaaggtcg gcgtgccgcc gccgaagtgc
     6301 agttgggcaa gctggtgccg tccgttcaga tgtggagcga gcagttccat ttctttttca
     6361 agatatttga tgtaggtatc ggcgcggctt ttgtctttgg tgatgatttt gttgcagccg
     6421 cagtagtagc agatggtgtt gcaaaacgga atgtgaatgt aaagggaaag cggtttgttt
     6481 aacgcgccca taccgcgcaa atgtaaaact ttgatatatt cgccttcgcg gaaaccgtca
     6541 tggaaacggt cggcggtagg gtaggaagta tagcgcgggc cgctggcggg caggctggca
     6601 atcagcgcgc ggtcaaactc ggggcggtca tcgtttacat tgtgattgtt ctgtatctga
     6661 atgattttca tggtgtgtgt gcggttttat gatgttagtc aaattttgga tagtttggta
     6721 gaatgccaca gtatgataaa cctgtcttga tatgtgtcaa caagcacata atcgggatgg
     6781 ggcaaaacac acgaaaaggt acggtatggc ttcgcataat actacacatc agatgaaaac
     6841 gctgtgttct tcctgttctt tgcgggaact ctgcctgcct gtcgggctgc tgcccaacga
     6901 gttcagccaa ctcgatgccg tcatccgcca aagccgccgc ctgaaaaaag gcgaatacct
     6961 gttccgtgcc ggcggagcct ttacctcgct ctttgccatc cgttcgggct tcttcaaaac
     7021 aaccgtcgcc agtcaggacg gccgcgatca ggtaacgggt ttctttatgt cgggcgaact
     7081 catcggcatg gacggcatct gttcctatgt gcacagttgc gacgcggtcg ccttggaaaa
     7141 cagcgaggta tgcgagttgc cgtttaccca tatcgaagag ctcggtcaga atatccccag
     7201 cctgcgtaca cactttttcc gcatgataag ccgcgaaatt gtgcgcgatc aaggtgttat
     7261 gctgttgttg ggcaatatgc gcgccgaaga gcggattgcc gccttcctgt tgaacctttc
     7321 ccaacgcctt tattcccgag gtttcgccgc caacgacttc atcttaagaa tgtcccgcga
     7381 agaaatcggc agttatctcg ggctgaaact tgaaaccgtc agccgcacat tatccaagtt
     7441 ccatcaggaa ggattgattt ccgtcgaaca caaacacatc aagattctca atctgcaggt
     7501 gctgaaaaaa atggtatccg gttgttcgca cgccatttga ttaacctgta cgaacatttc
     7561 agacggcatt ctcaataaac acagggcaga cgaaaacatc tgccctgttt gttgtatctg
     7621 ccgcaaagtg ccgcctgaaa accggcagcc gcctaaatcg aaaaatcctc gctgatgggc
     7681 gtgtacagaa tcctatccac cttctcgcgt gtcaggtgcg gcgcaaacgc ttggataaag
     7741 tcgtaggcat atccgcgcaa ataagtatcg ctgcgcaaag ctatccacgt cggcgacggc
     7801 tcgaacaggt gtgccgcatc cacaagctgc aaatcgccgt ccgtatccgg gttgtatgcc
     7861 attttcgcca tcagtcccac gcccaaaccc aagcgcacat aagtcttcaa tacatccgta
     7921 tccgccgccg ccaatgcgac atccggccgt tccaaacggg ctttggcgaa tgcccgcgcg
     7981 atgctgctgc ccgcattgaa tgcaaattca taagtaatca gcggaaacct cgccaaatct
     8041 tcaatacgga ggggattcct gcattcgagc agcggatggt cgttcggcac aatcaccgcg
     8101 tgtgtccagt cgtagcagga aagccttccc aactcggggt ggtcgtctat ccgttccgta
     8161 acaatcgcca aatcagattc tcccgaagta accatttggg cgatggcggc agggcttccc
     8221 tgtttgatgg tcagattgac tttcggatag cgtttcacaa aatcggcaac aatcaacggt
     8281 agggcatagc gcgcctgcgt atgcgtcgtg gcaaccgtca gcgaaccgct gtcctgtccg
     8341 gtaaactcgc tgccgatatt tttaatgttc tgtacatcgc gcaaaatacg ttccgcaata
     8401 tccaaaacca ccttgcccgg ctgcgagacc gaaaccacgc gcttgccgct gcggataaaa
     8461 atctgaatgc cgatttcttc ttccagcaat ttgatttgtt tggagatgcc gggttgtgat
     8521 gtgaataagg cttcggccgc ttcggaaacg ttcaggttgt gccggtaaac ttctaaggcg
     8581 tatttcaatt gttgtaattt catggcgggt cggtgtgggt ctgtgtcggg tggctgaaca
     8641 ttgtttataa tttatcatat tttcttgccg gtacggtatg gggctttgcc gttgtgtttg
     8701 ttgtttttgt gcaacggcaa tcgtgcgata tggaaaaaat ccccctaaag taatgacacg
     8761 gaattgattt ttcggcatga tagactatca ggaaacaggc tgttttacgg ttgttttcag
     8821 gcgttgagta ttgacagtcc gccccctgtt tctttatagt ggagactgaa atatccgatt
     8881 tgccgccatg tttctacagc ggcctgtatg ttggcaattc agcagttgct tctgtatctg
     8941 ctgtacaaat ctaatgaggg aataaaatga ccaaacagct gaaattaagc gcattattcg
     9001 ttgcattgct cgcttccggc actgctgttg cgggcgaggc gtccgttcag ggttacaccg
     9061 taagcggcca gtcgaacgaa atcgtacgca acaactatgg agaatgctgg aaaaacgcct
     9121 actttgataa agcaagccaa ggtcgcgtag aatgcggcga tgcggttgcc gtccccgagc
     9181 ccgaacccgc gcctgtcgcc gttgtggagc aggctcctca atatgttgat gaaaccattt
     9241 ccctgtctgc caaaaccctg ttcggtttcg ataaggattc attgcgcgcc gaagctcaag
     9301 acaacctgaa agtattggcg caacgcctga gtcgaaccaa tgtccaatct gtccgcgtcg
     9361 aaggccatac cgactttatg ggttctgaaa aatacaatca ggctctgtcc gaacgccgcg
     9421 catacgtagt ggcaaacaac ctggtcagca acggcgtacc tgcttctaga atttctgctg
     9481 tcggcttggg cgaatctcaa gcgcaaatga ctcaagtttg tcaagccgaa gttgccaaac
     9541 tgggtgcgaa agcctctaaa gccaaaaaac gtgaggctct gattgcatgt atcgaacctg
     9601 accgccgcgt agatgtgaaa atccgcagca tcgtaacccg tcaggttgtg ccggcacgca
     9661 atcatcacca acactaaggc taggtaatat cttgccgatg catgaggtta gcggattttg
     9721 taccgggtac tgttgcaata ttcgtgaaac gtcggccggt atcgatgatg tgaaacaaac
     9781 cccgcttttg cggggtttgt ttttttgggt ggttttctga aacggctatc gtcagaatcg
     9841 gggtgcaggt tcggattcgg attcagattc atgtttgtgt cccattgccg cgctttatag
     9901 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaata gtacggcaag
     9961 gcgaggcaac gccgtaccgg tttaaattta atccactata tcggttgaaa ctctgatttt
    10021 aaggcggtag gatgtgggtt tgcccatagc aagggaatcc tttctgtatc aagccccgaa
    10081 agggataatt catacaaatt cacgcctttc cccctcattg ggaaatggat ggaatcgtgc
    10141 ccgatgtgtg cggcactgta tgccggatat ggttttatca tcatcccttt tcggttgaaa
    10201 ccccgccagt tgcagcgatt gagcctaatc ggtggcggaa gttgccgctt tgcattcggg
    10261 gcggcgtgca gtgcggtgct ttgatatgcc gtttgtgtgt tgaaacaggg tggtcggtgc
    10321 atacgggtac ggtatggcta aagctaaaag tgaaatacgc tgaaacactg aatgagccgc
    10381 tttattgttt gtacgacctt tgctgctttg ctatgattta aattggattc gcacgccgga
    10441 tattttggga tatgaaagaa ttcgacttca tcaaacggta tttgcaaaca ggtacggata
    10501 aggatgtcgt attgggcata ggcgacgatg cggcgattgt ccgcccgcgc gaaggcttcg
    10561 atttgtgttt cagtgcggat atgcttttga aggacaggca tttttttgca gatgtcaaac
    10621 ctgaaggctt ggcttggaag gttttggccg tcaatatttc agatatggcg gcgatgggtg
    10681 cgataccgcg ttgggtgctg ctgagcgcgg cgttgcccga attggatgaa gtatggctgg
    10741 aacggttttg cggcagcttt ttcggtttgg caaaaaagtt tggcgtaacg ttaatcggcg
    10801 gcgatacgac caagggcgat atggcgttca atgtaaccat tatcggcgaa ttgccgaagg
    10861 gtagggcgtt gcggcgtgat gcggcggttg cgggcgacga tatttgggtg tcggggcgtg
    10921 tcggtatggc ggcggcggct ttgaactgcc gtctgaaacg gtgtgtgttg ccggatgatg
    10981 tgttcgccga atgcgaacaa aagctgctcc gtcccgagcc aagggttggg ctggggcttg
    11041 cgctgttgcc gtttgcccgg gcggcacagg atgtttcaga cggccttgcg caagatttgg
    11101 ggcatatcct gaccgcttcc ggcgtggggg cggaaatttg ggccgattcg ctgccatctt
    11161 tatctgtatt gaaagatatt ttgccccgag cgcaatggct gtcttatact ttggcgggcg
    11221 gcgacgatta cgagctggtt tttaccgctc cggaaagttg ccgcagccgc gtgcttgatg
    11281 cggcggaacg gtgcggcgtg ccggtaacgt gcatcggcaa aatcaacgga ggatgccgtc
    11341 tgaaggtttt agatgccggc ggcagggaat tggaactaca ttctttagga ttcgatcatt
    11401 ttggctgatt ttaaacctga ctttgcgtgg ctgttgaaac ggccgttgtg ttttttggct
    11461 ttcggtttcg gcagcgggct ggctccgttt gcgccgggcg cattcggcac tttggcggca
    11521 ctgcctttgg cgtttgtgct gattttgctc ggcatagacg ggctgctgct ggcttttgtg
    11581 tgtatcgtgc tgtttatgtg gggcatacgt atttgtgcgt gtgcggaacg tgaaacgggt
    11641 gtcagtgacc acggtgggat tgtttgggac gagattgtcg ccatgctgtt tgtgctggcg
    11701 tttgtgccgt tcaggtggac gtggtggctg gcggcatttg ttctgttccg tctgtttgat
    11761 gcgctcaaac cgccccccgt cggttggttt gacaagaatc tgcacggcgg tttgggcatt
    11821 atggcggacg atatggcggc tgcggtgatg accttgattg tcttgaggat tgcaatgctg
    11881 ttttaaacgg ggctgccttg taaaaatgcc gtctgaaagc ctttcagacg gcattgtttc
    11941 ggaggttaac gcgttaccgg tttgtatttg atgcgtttcg gtttcgcgcc ttcttcgccg
    12001 agtcggcgtt tcttgtcggc ttcgtattct tgatagttgc cgtcgaagaa cacccatttg
    12061 gagtcgcctt cacacgccaa gatatgcgtg gctatgcggt cgagaaacca gcggtcgtgc
    12121 gaaatcacca tcacgctgcc ggcaaattcc aacaatgcgt cttccaacgc gcgcagggtt
    12181 tccacgtcga gatcgttgga cggttcgtcc agcagcaaca cattgccgcc gcccaacaag
    12241 gtttttgcca agtgcagacg gccgcgttcg ccgccggaaa gctgccttgc gattttgctt
    12301 tggtcgctgc ctttaaagtt gaagcgtccc aaatattggc gggcggggat ttcaaactgt
    12361 ccgacctgca aaatatcgcg accttcggca atgttgtcga acacggtttt gtcgttttgc
    12421 aaaccttcgc ggctttggtc aatcaagctc attttcacgg tttgcccgat tttcacttcg
    12481 cccgaatcgg gctgctcttt gcccgcaatc attttgaaca gcgtcgattt acccgcgccg
    12541 ttcgggccga tgatgccgac aatcgcgccc gccggcactt tgaagctcaa atcgtcaatc
    12601 agcactttat cgccgaacga tttggaaaca ttcacaaatt caatcacttc gttacccaaa
    12661 cgctcggcaa cagggataaa gatttcctga gtttcgttgc gtttttggta ttcgtagttg
    12721 ctcatttctt caaaacgcgc caaacgcgct ttgggcttgg cttggcggcc tttggcattt
    12781 tggcgcaccc attccaattc ctgcttcatc gccttcacgc gcgcggcttc ggatttcgcc
    12841 tcgttttcca agcgtttttc tttctgctcc agccaagacg agtaattgcc tttccacgga
    12901 atgccgtgtc cgcggtcgag ttccaaaatc cattcggcgg cgttgtcgag gaagtagcgg
    12961 tcgtgcgtta ccgcgaccac tgtgccgggg aagcgcacga ggaattgctc cagccattcc
    13021 accgattccg catccaagtg gttggtcggc tcgtccaaga gcagcatatc gggcttgctc
    13081 aacaaaagtt tgcacaaagc gacgcggcgt ttttcaccgc ccgaaagatt gccgattttg
    13141 gcatcccaat caggcaggcg cagcgcgtcg gcagcgattt ccaattcgtg ttccgcaccg
    13201 ccgccagttg acgaacccgc cgcgataatc gcttccaagc ggccttgttc ttccgccagc
    13261 gcgtcaaaat ccgcatcggg attggcgtac tcggcataca cttcttccaa acgtttctgt
    13321 gcggcagcca cttcgccgaa accgctttcc acttcctcgc gcacggtttt ttccggatca
    13381 agctccggct cttgcggcag atagccgatt ttgatgtcgc ccatcggcac ggcttcgccc
    13441 tcaaattctt tatccacgcc cgccataatc cgcagcacgg tggacttgcc cgtgccgttc
    13501 aagcccaaga ggccgatttt cgcgccgggg aagaaagaaa gggaaatatc tttaatgatg
    13561 gttttctgcg gcggcacaac cttgctcacg cgcagcatag aatagacgta ttgttgggac
    13621 atagttttct cgattcggtc aaacaaattt cagacggcca ttttaaccga taatttgatt
    13681 taagccagtt tatccgcgaa ccggtattgc caaaatcggg caggattcat aaaatccgcc
    13741 tatccctttg aaattataca gacaaaaaaa taataatgat aggggatcgc cgcgccggca
    13801 accatttcgg attttccaaa gcaaatactt ttcaaatcaa aaaaaaggat ttactttatg
    13861 tcggaatata cgcctcaaac agcaaaacaa ggtttgcccg cgccggcaaa aagcacgatt
    13921 tggatgttga gcttcggcta tctcggcgtt cagacggcct ttaccctgca aagctcgcag
    13981 atgagccgca tttttcaaac gctaggcgca gacccgcaca atttgggctg gtttttcatc
    14041 ctgccgccgc tggcggggat gctggttcag ccgatagtgg ctactactca gaccgcactt
    14101 ggaagccgcg cttgggcggc cgccgcctgc cgtatctgct ttacggcacg ctgattgcgg
    14161 tcatcgtgat gattttgatg ccgaactcgg gcagcttcgg tttcggctat gcgtcgctgg
    14221 cggccttgtc gttcggcgcg ctgatgattg cgctgttgga cgtgtcgtcg aatatggcga
    14281 tgcagccgtt taagatgatg gtcggcgata tggtcaacga ggagcagaaa agctacgcct
    14341 acgggattca aagtttctta gcgaatacgg acgcggttgt ggcagcgatt ctgccgtttg
    14401 tgttcgcgta tatcggtttg gcgaacactg ccgagaaagg cgttgtgcca caaaccgtgg
    14461 tcgtagcatt ctatgtgggt gcggcgttac tgattattac cagtgcgttc acaatctcca
    14521 aagtcaaaga atacgacccg gaaacctacg cccgttacca cggcatcgat gtcgccgcga
    14581 atcaggaaaa agccaactgg ttcgaactct taaaaaccgc gcctaaagtg ttttggacgg
    14641 ttactccggt acagtttttc tgctggttcg ccttccggta tatgtggact tactcggcag
    14701 gcgcgattgc agaaaacgtc tggcacacta ccgatgcgtc ttccgtaggc catcaggagg
    14761 cgggcaaccg gtacggcgtt ttggcggcgg tgtagtcggt tgcggcggtg atttgttcgt
    14821 ttattctggc aaaagtaccg aataaatacc ataaggcggg ttatttcggc tgtttggctt
    14881 tgggcgcgct cggtttcttc tctatcttct tcatctacaa tcaatacgca ctcatcctgt
    14941 cttatatctt aatcggcatc gcttgggcgg gcattatcac ttatccgctg acgattgtgg
    15001 ccaacgcttt gtcgggcaaa cacatggata cttatttggg cctgtttaac ggctctgtct
    15061 gtatgccgca aatcgtcgct tcgctgttga gtttcgtgct tttcccgatg ctgggcggcc
    15121 atcaggcaac catgttcttg gttgcaggcg cagtcttgct gctgggagcc ttctcagtct
    15181 gtctgattaa agagacccac ggcggggttt gagcgatgag cgataccccc gctacccgcg
    15241 atttcggcct gatcgacggg cgggccgtaa ccggctatgt gctgtccaac cggcgtggta
    15301 cgtgcgtctt cgtgctggac ttgggcggga ttgtgcagga attttccgtt ttggcagacg
    15361 gcgtgcgcga aaaccccgtg gtgtcgttcg acgatgcggc ttcctatgcg gacaatccgt
    15421 ttcagattaa caagcagata gggcgcgtgg ccggacgcat ccgcggtgcg gcgttcgaca
    15481 tcaacggtag gacttaccgc gtggaggcca acgaaggcag gaacgcgctg cacggcggtt
    15541 cgcacgggct ggccgttacc cgtttcaacg cggtggcggc agacggccga cggttatccc
    15601 aacgatttgg atatttccta ccgcttggac gaggatggcc ggcttaccgt tacctatcgc
    15661 gccaccgcgc tcggcgacac ggtgttcgac ccgacgctgc acatttactg gcggctggac
    15721 gcgggcctgc acgatgcggt tctgcatatt ccgcagggcg gacatattcc ggccgatgcc
    15781 gaaaaactgc ccgtcttaac ggtttcagac ggcctcgaag tatttgattt cagccggccc
    15841 aagccgccgg atgccgccgt tgccgccctg cgccgcgaaa cgggccggtt ttgacgacgc
    15901 ttaccgcgtg ccgtccgata taggccgtcc cgccgccgtg ttgcaagccg gacgccgccg
    15961 tcgtatcagc atatacagcg accgcaacgg cttggtcatc tttaccgccg ccccgcagga
    16021 tttcgcgcgg cacgatgcgg gcgtttacga cgcgctggcg accgaggcgc agacgctgcc
    16081 cgacagcctg cattggcccg aattcggcaa tatccgtctg aacaagggtg atactaggga
    16141 ggcgacgatt gcttacggca tcgaatccct ttcttaggag cttcctaaca ccggtttcag
    16201 acgacctttt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttgccgtact
    16261 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca cgccttgccg
    16321 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactataa
    16381 gatttcacca ttccctcaaa tcaatccaaa caggagcttt acgatgtaca caagaattat
    16441 ggaaatcagc ccttggacgc tgcgttcggc aaaactggaa aaagaacaca aacggctgca
    16501 agagagcctg accagcttgg gcaacggcta tatgggtatg cgcggcagct ttgaggaaac
    16561 ctactccgcc gacagccact tgggcaccta catcgccggc gtgtggtttc ccgacaaaac
    16621 ccgcgtcggc tggtggaaaa acggctatcc caaatatttc ggcaaagcca tcaacgcgct
    16681 caatttcagc aaagtcaaaa tctttgtcga cgggcaagag gtggacttgg cgaaaaacga
    16741 cgttgccggc ttctccgtcg aactggatat gcaacacggc gtgttgcgcc gctcgttcac
    16801 cgtattcggt gtgcgtttcg atgtgtgcaa attcctgtcg gtcgcgcaaa aagagctggc
    16861 tctcacccgc tgggaagccg tgtccgttga cggcaaaacc caccaagtcc gcatcgattc
    16921 catcatcgat gccgacgtga aaaacgaaga ctccaactac gaagaaaaat tctggcaggt
    16981 attggacaaa ggcgtttcag acgaccgctc ctacattgcc gcccaaaccg tcgccaaccc
    17041 tttcggtgtg gaacaattta tcgtcaacgc cgggcaaacc ttcgccggca gcttcaaagc
    17101 cttcggcggc agccaaaccg attggcaggt ctctaattct tttgaagccg aagtcggcgg
    17161 cacgcccgaa acctttgaaa aacgcgtgat tgttaccacc agccgcgatt atcagggctt
    17221 ggaagcagtg aaagccgccg gccgcgcctt atctgaaaaa atcgcaggtg ttgcgtttga
    17281 aaccttgctg gatgcgcaca aagcaggctg gcagcatcgt tgggaaatcg ccgacgtggt
    17341 catcgaaggc agcgacgaag cacagcaggg catccgcttc aacctgttcc aactgttctc
    17401 cacctactac ggcgaagacg cgcgtctgaa catcggcccc aaaggcttta ccggcgaaaa
    17461 atacggcggc gcgacctatt gggacaccga agcctacgcc gtgccgctct accttgcact
    17521 ggcggaaccc gaagttaccc gcaacctgct gcaataccgc cgcaaccaaa ttgccgcagg
    17581 tgcagcataa cgcacgcgaa cagggcttgg cgggcgcact ctatccgatg gtaacgttta
    17641 cgggcatcga gtgccacaac gaatgggaaa tcaccttcga ggaaatccac cgtaacggcg
    17701 cgattcctta cgccatctac aactacacca actacaccgg tgacgagtgc tatcttgcca
    17761 aagaaggctt ggaagtcttg gtcgaagtgt cccgcttccg ggcggaccgc gtccacttct
    17821 ccaaacgcaa cggcaaatac atgattcaag gcgtaaccgg tccgaacgaa tacgaaaaca
    17881 acatcaacaa caactggtaa accaacaccc tcgccgcatg ggtattgggc tacacccgcg
    17941 aagccttggc gaaatacccg cgtccgggtt cgaacgtgag cgccgccgag ttggaaaaat
    18001 gggcggacat cagcgcgaat atgtaccgtc cgcatgacga agaactcggc gtattcgtgc
    18061 agcacgacgg cttccttgac aaagacatcc gccccgtatc cgcgctttcg cccgacgatt
    18121 tgccgctcaa ccagaaatgg tcgtgggaca aaatcctgcg ttcgcccttc atcaaacagg
    18181 cggacgtatt gcaaggcatc tacttcttcg gcgaccgttt cgacatggac gaaaaacgcc
    18241 gcaacttcga cttctacgaa ccgatgaccg tgcatgaaag ctcgttgtct ccatgtatcc
    18301 actccatcct cgctgccgaa ctgggcaaag aagaaaaagc cgtggaaatg taccggcgca
    18361 ccgcccgcct ggacttggac gactacaaca acgacaccga agacggcctg catattactt
    18421 ctatgaccgg ctcgtggctc gccatcgtcc aaggtttcgc ccaaatgaaa acttggggcg
    18481 gcaaactcag cttcgcaccg ttcctgccga gtgcgtggac aggctatgcc ttccacatca
    18541 actaccgcgg ccgtctgatt aaagtcgccg tcggcaaaaa acgtcgtctt caccctgctc
    18601 aaaggcgatc cgctcgaatt gcaggtgtac ggcaaagata tcacgctcaa cggcagccac
    18661 accgttgcat tggaaaaata aggagggcgc aaaatgactt ttaccgcagt gctcttcgat
    18721 ctcgacggcg tgattaccga taccgccgaa taccactacc tcgcatggaa aaagcttgcc
    18781 gaagaattgg gcatcggcat cgaccgcaag tttaacgagc agctcaaagg cgtgtcgcgc
    18841 gacgattcgc tcaaacgcat cctcgcgcac ggcggcaaaa ccgtcggcga aaccgagttc
    18901 gccgaactga cctgccgcaa aaacgacaac tacttagaga tgattcaggc agtcaaaccc
    18961 gaagacgtgt accccggcat tttgccgctg ctggaagcat tgagggcaaa cggcaaaaaa
    19021 atcgccctcg cgtccgccag taaaaacggc ccgttcttgc tcgaacgcat ggggctgacc
    19081 cacttcttcg acaccgtcgc cgaccctgcc gccgtcgcgc attccaaacc ctcccccgac
    19141 atcttccttg ccgccgccga gggcgtgggt gcggacatac gccgctgccg gcgtcgccgc
    19201 catcaaagcc tctggcgctt tgcccatcgg cgtgggcaaa accgaagact tgggcagcga
    19261 catcgcgctg gtgtccgaca ccgccgagct gacctacgcc tacttgcaaa atgtgtggga
    19321 acagtcgggc agataaaatt ctaaggaagt aaaaaggccg tctgaacagt gtttcagacg
    19381 gcctttctgc ttttagaaca gaatgataa
//
LOCUS       DAWTHD010000026        19308 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWTHD010000026 DAWTHD010000000
VERSION     DAWTHD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.026"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            395..1237
                     /locus_tag="WM704_000745"
     CDS             395..1237
                     /locus_tag="WM704_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFA9340213.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1266..1730
                     /gene="nrdR"
                     /locus_tag="WM704_000746"
     CDS             1266..1730
                     /gene="nrdR"
                     /locus_tag="WM704_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HFA9340214.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            1762..2871
                     /gene="ribD"
                     /locus_tag="WM704_000747"
     CDS             1762..2871
                     /gene="ribD"
                     /locus_tag="WM704_000747"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFA9340215.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            3066..4487
                     /locus_tag="WM704_000748"
     CDS             3066..4487
                     /locus_tag="WM704_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFA9340216.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTTVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIP
                     LALSSLAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLLQSIFSTVWTPYIFR
                     AIEENATPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFTVVSCMLPP
                     LFYTLTEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGTRGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKNLHKLFHYLKKQGFPL"
     gene            4655..5896
                     /locus_tag="WM704_000749"
     CDS             4655..5896
                     /locus_tag="WM704_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFA9340217.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSATIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            6027..7202
                     /locus_tag="WM704_000750"
     CDS             6027..7202
                     /locus_tag="WM704_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HFA9340218.1"
                     /translation="MLNTALSPWPSFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7250..9160
                     /locus_tag="WM704_000751"
     CDS             7250..9160
                     /locus_tag="WM704_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFA9340219.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVTTVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVKITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            9216..10508
                     /locus_tag="WM704_000752"
     CDS             9216..10508
                     /locus_tag="WM704_000752"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFA9340220.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAIYCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(10560..11396)
                     /locus_tag="WM704_000753"
     CDS             complement(10560..11396)
                     /locus_tag="WM704_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFA9340221.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKRQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(11691..11894)
                     /locus_tag="WM704_000754"
     CDS             complement(11691..11894)
                     /locus_tag="WM704_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340222.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(11872..12237)
                     /locus_tag="WM704_000755"
     CDS             complement(11872..12237)
                     /locus_tag="WM704_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFA9340223.1"
                     /translation="MAAYGVSDGIFTEQAVRTHRRFCGNFGCGGGRCYNNALFLKGDI
                     MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNGGTEIRKTAKIRGGE
                     VIEFDGARLEIADGYDPEA"
     gene            12638..16072
                     /gene="dnaE"
                     /locus_tag="WM704_000756"
     CDS             12638..16072
                     /gene="dnaE"
                     /locus_tag="WM704_000756"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFA9340224.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            complement(16724..17346)
                     /locus_tag="WM704_000757"
                     /pseudo
     CDS             complement(16724..17346)
                     /locus_tag="WM704_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(17334..17654)
                     /locus_tag="WM704_000758"
     CDS             complement(17334..17654)
                     /locus_tag="WM704_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340225.1"
                     /translation="MRSQPEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMQSEGYPVLFVLDGEAFFPRCTT
                     SCSR"
     gene            complement(17724..18267)
                     /locus_tag="WM704_000759"
                     /pseudo
     CDS             complement(17724..18267)
                     /locus_tag="WM704_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(18213..18428)
                     /locus_tag="WM704_000760"
     CDS             complement(18213..18428)
                     /locus_tag="WM704_000760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340226.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            18588..19247
                     /locus_tag="WM704_000761"
     CDS             18588..19247
                     /locus_tag="WM704_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFA9340227.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
BASE COUNT         4303 a         5928 c         5087 g         3990 t
ORIGIN      
        1 agcaaccctg tcgccgtcat tcccgcgaaa gcgggaatcc agtccgttca gtttcggtca
       61 tttccgataa attcctgttg cttttcattt ctagattccc actttcgtgg gaatgacggc
      121 ggaagggttt tggttttttc cgataaattc ttgaggcatt gaaattctag attcccgcct
      181 gcgcgggaat gacggctcaa aagttacgga acgaaaaaca accgaaaccg gacaagtcgg
      241 attcccgcct gcgcgggaat gacggaatct taagtttccg tctttgtttt ctgttttcgc
      301 gggaataatg gggaacaagc cgtatttcag acggcatttt cagttcgggg tataatccga
      361 atacttgcga ccatctgaat catttggaca aactatgtgt caactgctgg gcatgaactg
      421 caatacgccg accgatatta tgttttcctt tgaaggcttc cgccgcaggg gcggcattac
      481 cgaccatcac gccgacggtt tcggcatcgg ctttttcgaa ggcaaaggcg tgcgcctgtt
      541 ccatgacgac aagccgagtg caaattcccc cgtcgccgac ctcgtgcgcg cctaccagat
      601 taaatcggaa aacgtcgtcg cccacatccg caaagcctcg caaggtcaaa cctcgctggc
      661 gaacacccat ccctttatgc gcgaaatgtg gggcggctac tggctgtttg cccacaacgg
      721 gcatttggtc gattttttcc ccgaacaagg agaatttttc caccccgtcg gcacaaccga
      781 ttccgaacgc gcgttctgcc acatcctcaa ccgcctgcgc acccgctttg ccgcccgtcc
      841 cgacgacgac acgctgtttg acgcgattgc ggggctgacg cacgaaatcc gcaagttcgg
      901 gctgtttaac ttcatgcttt cagacggcat ttccctgttt gcccacgcca gcacgctgct
      961 gcactacatc gtccgccaag ccccgttcgg caaagcgcgc ctgctcgacg acgatgtaat
     1021 ggtcgatttt gccgaagtaa ccacgccgga cgaccgcgtc gccgttatct ccaccctgcc
     1081 gctgactcgc gatgaatcgt ggtcccaact tgccgtgaac gaactggtca tgttccgcga
     1141 aggcaacatc gtccggcacg accgtcccga aaatcccgtc tatatgagtg ccgaagaagg
     1201 cctggaaatc gcccgcgccg ccggcgtatc ggtttgacgt tcagacggca tcaggaagaa
     1261 acaccatgaa atgcccgttt tgcagccacc cagacacccg agttgccgat tcccgcctga
     1321 tggaagagcg caataccgtc cgccgccgcc gccactgccc cggctgcggc aaacgcttcg
     1381 gcacgctcga aaccgtcgaa ctcaaaatgc ccgccgtcat cggtccggac aagaaacgtt
     1441 cgcccttcaa tgcacaacgc ctccgcaacg acctgaccgc cgccgcccga aaatccgccc
     1501 tgacacccga acagattgac gaaaccgtcc gcctgacgga acacaggctc tacacttcgg
     1561 gtcagcgcga catatcctca tccgaacttg tcgaaatcgt gctggaggag ctgttcgggc
     1621 aaaacacgga agccgccgtc cgtttcgccg ccctgcacaa acgcttcgac agcccggaac
     1681 atttcgcctc gtggctggcg caagccgtca aaacaggcgg caaaacctga ttcccccaac
     1741 ccatactgat acggtatccc tatgttttcg gacacaaata tatccatgat ggaaaacgcc
     1801 ctccgacttg ccgctttggg gcgtttttcc acttcaccca acccgcgcgt cggctgcgtt
     1861 atcgcacacg gcagacaaat tgtcggacag ggcttccacg tcaaagcggg cgaaccccat
     1921 gccgaagtcc acgccctgcg tcaggcgggg gaaatggcaa aaggcgcgac cgcctttgtt
     1981 accctcgaac cgtgcagcca ttacggacgc acaccgccct gcgccgaagc cctgctccgt
     2041 tccggcgtaa cacgcgtcgt cgccgccatg cgcgacccca acccgccggt tgcaggcaaa
     2101 gggcttgtcc tgctcaaagc agcgggcatc aagacggaat gcggactact tgaaaacaaa
     2161 gcacgcgaac tcaaccgggg atttttatcg cgcatcgaac gccgccgccc ctttgtccgc
     2221 ctcaaatgcg ccgtttcgct ggacggcaaa accgcccttt cagacggcag cagcttttgg
     2281 attaccggcg aggaagcgcg tgccgacgta caggttttgc gtgccgaaag ctgcgcggtg
     2341 ctgaccggca tcggcacggt gttggcagac aatccccggc tcaacgtccg cgcttttcca
     2401 actttgcgcc aacccgcacg catcgtttta gacagccgcc tgcgcctgcc cccgaacagc
     2461 catttggtta ccgacggaca atctccgacc tacatcgcca ccttggaacg cgatgaagac
     2521 aaactccgcc cctatcggga acacgcacac atccgcatcc tgatgccgtc tgaaacggca
     2581 gacggcaaaa tcgacctgca ccatctgatg cgcctccttg ccgacgaagg tttcggcgaa
     2641 atcatggtcg aagcaggctc cgaactcaca tccgcatttt tggcggaaaa tttggcggac
     2701 gaaatcgtgc tgtaccgttc gcccaaaatc ctcggcggcg gcaaagacct gttttccctg
     2761 cccgaaaacc gtgccgccct ttccgcaccg cccttgtgga cacccgtttc aagcgaaatc
     2821 ctcggacacg acatcaaaac cgtgttccgg aaaaacggca acgcctttta aagggtttgc
     2881 cgccgttttg ctatataata acgccgataa aaaacggccc gttcagaccg ccgcccgccc
     2941 ggaaaagcgc aacccggact gccgcacccc gccggcagcc gcgacggcct gaaagccgtc
     3001 aaattccgat caagaaaggc ttcagacggc acaggcagca tcacgccgcc gcccggacat
     3061 caaaaatgga cacaaaagaa atcctcggct acgcggcagg ctcgatcggc agcgcggttt
     3121 tagccgtcat catcctgccg ctgctgtcgt ggtatttccc cgccgacgac atcgggcgca
     3181 tcgtgctgat gcagacggcg gcgggactga cggtgtcggt attgtgcctc gggctggatc
     3241 aggcatacgt ccgcgaatac tatgccgccg ccgacaaaga cactttgttc aaaaccctgt
     3301 tcctgccgcc gctgctgttt tccgccgcga tagccgccct gctgctttcc cgcccgtccc
     3361 tgccgtctga aatcctgttt tcgctcgacg atgccgccgc cggcatcggg ctggtgctgt
     3421 ttgaactgag cttcctgccc atccgctttc tcttactggt tttgcgtatg gaagggcgcg
     3481 cccttgcctt ttcgtccgcg caactcgtgc ccaaactcgc cattctgctg ctgttgccgc
     3541 tgacggtcgg gctgctgcac tttccggcga acaccgccgt cctgaccacc gtttacgcgc
     3601 tggcaaacct tgccgccgcc gcctttttgc tgtttcaaaa ccgatgccgt ctgaaggccg
     3661 tccggcgcgc gccgttttcg cccgccgtcc tgcaccgggg gctgcgctac ggcataccgc
     3721 tcgcactgag cagccttgcc tattgggggc tggcatccgc cgaccgtttg ttcctgaaaa
     3781 aatatgcggg cctggaacag ctcggcgttt attcgatggg tatttcgttc ggcggggcgg
     3841 cattattgct ccaaagcatc ttttcaacgg tctggacacc gtatattttc cgtgcaatcg
     3901 aagaaaacgc cacgcccgcc cgcctctcgg caacggcaga atccgccgcc gccctgcttg
     3961 cctccgccct ctgcctgacc ggaattttct cgcccctcgc ctccctccta ctgccggaaa
     4021 actacgccgc cgtccggttt accgtcgtat cgtgcatgtt gccgccgctg ttttacacgc
     4081 tgaccgaaat cagcggcatc ggtttgaacg tcgtccgcaa aacgcgtccg atcgcgcttg
     4141 ccaccttggg cgcgctggcg gcaaacctgc tgctgctggg gcttgccgta ccgtccggcg
     4201 gcacgcgcgg cgcggcggtt gcctgtgccg cctcattctg gttgtttttt gttttcaaga
     4261 cagaaagctc ctgccgcctg tggcagccgc tcaaacgcct gccgctttat atgcacacat
     4321 tgttctgcct ggcctcctcg gcggcctaca cctgcttcgg cacaccggca aactaccccc
     4381 tgtttgccgg cgtatgggcg gcatatctgg caggctgcat cctgcgccac cggaaaaatt
     4441 tgcacaaact gtttcattat ttgaaaaaac aaggtttccc attatgaaaa tcatcctgac
     4501 tacttctatg tcggttctgg gaggcacggg cacgacatca tcgattgcca aatgtcgtgc
     4561 aaaaccgacc ccgttcagtc cgacgaaatc gtccgccgct tcaggcgcga catttcctat
     4621 cggaaaatcg tcaacctgat tgaaagattg gcaaatgagt aaagccgtca aacgcctgtt
     4681 cgacatcatc gcatccgcat cggggctgat tgtcctgtcg cccgtgtttc tggttttgat
     4741 atacctcatc cgcaagaatc tgggttcgcc cgtcttcttc attcgggaac gccccggaaa
     4801 ggacggaaaa ccttttaaaa tggtcaaatt ccgttccatg cgcgacgcgc ttgattcaga
     4861 cggcattccg ctgcccgaca gcgagcggct cacgcccttc ggcaaaaaac tgcgcaccgc
     4921 cagtttggac gaactgcctg aactgtggaa cgtcctcaaa ggcgagatga gcctggtcgg
     4981 cccccgcccg cttttgatgc agtatctgcc gctttacaac aaatttcaaa accgccgcca
     5041 cgaaatgaaa ccgggcatta ccggctgggc gcaggtcaac gggcgcaacg cgctttcgtg
     5101 ggacgaaaag ttctcctgcg atgtttggta caccgacaat ttcagctttt ggctggatat
     5161 gaaaatcctg tttctgacag tcaaaaaagt cttgattaaa gaaggcattt cggcgcaagg
     5221 ggaagccacc atgccccctt tcgcggggaa tcgcaaactc gccgttatcg gcgcgggcgg
     5281 acacggcaaa gtcgttgccg agcttgccgc cgcactcggc acatacggcg aaatcgtttt
     5341 tctggacgac cgcacccaag gcagcgtcaa cggcttcccc gtcatcggca cgacgctgct
     5401 gcttgaaaac agtttatcgc ccgaacaatt cgacatcacc gtcgccgtcg gcaacaaccg
     5461 catccgccgc caaatcaccg aaaaagccgc cgcgctcggc ttcaaactgc ccgttctgat
     5521 tcatcccgac gcgaccgtct cgccttctgc aacaatcgga caaggcagcg tcgtaatggc
     5581 gaaagccgtc gtacaggccg gcagcgtatt gaaagacggc gtgattgtga acactgccgc
     5641 caccgtcgat cacgactgcc tgcttgacgc tttcgtccac atcagcccgg gcgcgcacct
     5701 gtcgggcaac acgcgtatcg gcgaagaaag ccggataggc acgggcgcgt gcagccgcca
     5761 gcagacaacc gtcggcagcg gggttaccgc cggtgcaggg gcggttatcg tatgcgacat
     5821 cccggacggc atgaccgtcg cgggcaaccc ggcaaagccc cttacgggca aaaaccccaa
     5881 gaccgggacg gcataagcga ttaaaataaa cttccgtaca aaccgatttt gacaacacct
     5941 gcggcgcgcg cccgattctt cggaacacgc ccccttcaga cggcataggg tcggaaatgc
     6001 cgtccgaacc cgacggacca aacaccatgc tgaacaccgc cctttcccca tggccgagct
     6061 tcacccaaga agaagccgat gccgtttcca aagtcctgct gtccaacaaa gtcaactact
     6121 ggacgggcag cgaatgccgc gaatttgaaa aagaatttgc cgcctttgcc ggcacgcggt
     6181 acgccgtcgc cctttccaac ggcacgcttg cgctcgatgc cgcactcaaa gcaatcggca
     6241 taggcgcggg cgacgatgtg attgttacct cgcgcacctt cctcgcctcc gcctcctgca
     6301 ttgtgaacgc gggcgcaaac cccgtgtttg ccgacgtgga tttgaacagc caaaacatca
     6361 gcgcggaaac cgtcaaagcc gtgctgaccc cgaataccaa agccgtcatt gtcgtccacc
     6421 ttgccggtat gcctgccgaa atggacggca tcatggcttt ggcaaaagaa catgatttgt
     6481 gggtgattga agactgcgcc caagcgcacg gcgcgaccta caaaggcaaa tccgtcggct
     6541 ctatcgggca cgtcggcgcg tggtcgttct gtcaagacaa aatcatcacc accggcggcg
     6601 agggcggtat ggttacgacc aacgacaaaa ccctgtggga aaaaatgtgg gcgtacaaag
     6661 accacggcaa aagctacgat gccgtgtacc accgcgaaca cgcgcccggt ttccgctggc
     6721 tgcacgaaag tttcggcacc aactggcgca tgatggaaat gcaggctgtc atcggccgca
     6781 tccagctcaa acacctgccc gaatggacgg cgcgccgcca agaaaacgcc gccaagctgg
     6841 cagaaagttt gcgcaaattc aaaagcatcc gcctgattga agtcgccggc tacatcggac
     6901 acgcgcaata caagttctac gccttcgtca aacccgaaca cctgaaagac gactggacgc
     6961 gcgaccgcat cgtcagcgaa ctgaacgcgc gcaacgtccc ctgctatcaa ggcggctgct
     7021 ccgaagtgta tttggaaaaa gccttcgaca acacgccgtg gcggcccaaa gagcggctga
     7081 aaaacgccgt cgaactgggc ggcaccgccc tgacgttctt ggtgcacccg acgctgaccg
     7141 acgacgaaat cgcgttctgc aaaaaacaca tcgaagccgt cctgaccgaa gccgcacgat
     7201 aacccttcag acggcatatg ccgcctgaaa gcacataccg ccccacgata tgaatctgga
     7261 aaccctgatc gccctgccgc gcaacatcaa gaaaatctgt ttcctcatac acgattttct
     7321 gatgattttc accgcctttt ggttcaccca aagcctgaag gccgactact cggacgaatg
     7381 gttcgatttt gccaactggc agtctttttt gctgactgcc ttgctgacca tcacattatt
     7441 tatccgaatg gggctttacc gcgccgttac acgctttgtc agtttccacg tcctgaccac
     7501 cgcctttgcc ggcagcctcg cctccgccgt attgttcttc ctcaacacat tgatttttga
     7561 agaaaggctg cgcctcgccc tgccgattgt ctatttctta ctgctgtttg tttccgtgac
     7621 cggctcgcgt atggttttgc gcggactcct gtccgaccac cagaaaaaaa cacatgaccc
     7681 cgtcatcatt tacggcgcgg ggcgttcggg tcggcagctc cttgaagccg tcaaacagat
     7741 gcgcgaatat tccgccgccg cctttgtgga cgacaacccc aagctttggc acaccgtcat
     7801 ctacgacctt gccgtttacc agcccgatgc catcgccttc ctcatcgaac gctacggcgt
     7861 ggaaaaaatc ctgctcgcca ttcccagcgc gactcaggaa caacgccgcc gaatcatcag
     7921 caaactgaaa gcctatccgt gcgaagtgtt gaccattccc ggaatgaaag acctgatgga
     7981 cgggaaaatc agcatcggca cgctcaaaaa aatctctgtg tccgacctgc tcgggcgcga
     8041 ttccgtcgca cccgacgacc gcctcatgaa tgccgacacc gaaggcaaaa ccgtcatggt
     8101 aaccggcgcg ggcggctcca tcggttcgga actctgccgc cagattatcc gccgccgccc
     8161 cgaaaggctg ctgctgttcg agctgtccga attcgccctg tacaccgttg aaaaagaatt
     8221 gtgcgaatac tgcgcccgaa acggcatcgc cgccgaaatc cttccctttt tgggttcggt
     8281 gcaaaaccgc acgctgctca cgcacattat gaccgccttt tccgttacga ccgtctatca
     8341 cgccgccgcc tacaaacacg tccctatggt cgagttcaac accgtcgagg gcatacgcaa
     8401 caacatcttc ggcacactcg aatgcgcgct tgccgccacg gcttcgggcg tggaaacctt
     8461 cgtcctcatc tccaccgaca aagccgtccg ccccgccaac acgatgggtg cgggcaaacg
     8521 catggcggaa ctctgccttc aggcactcgc cgccgaaccc ggacaaaaaa cccgcttcag
     8581 catggtacgt ttcggcaatg ttttaggctc gtccggctcc gtcgtcccgc tgtttgaaaa
     8641 acagattgcc gaaggcggcc ccatcaccct gacccacccc gacatcacgc gctatttcat
     8701 gaccataccc gaagcggcgc aactggttat ccaagccggc gcgatgggca gaggcggcga
     8761 cgtgttcgtc ctcgatatgg gcgaatccgt caaaatcacc gaccttgccc gccaaatgat
     8821 taccttaagc ggactcaaac ccaaaacgcc cgaacagccc gacggcgaca tcgaaatcct
     8881 cattaccggg ctgcgccccg gcgaaaaact ctacgaagag ttgctcatcg gcgacaacgt
     8941 ccgcaaaacc ggccatccgc gcatcatgac cgccgacgag accatgctgc cgtggcacga
     9001 actctccgcc ctgctcgacc gcatccgcac cgcctgcgac cgttacgacc agcaggcaat
     9061 ccgcgacctg ctcgtcaacg ccccgaccgg ctttaccccg accggcggca tctgcgacct
     9121 gctttgggta tgcgaaacac acagaaaaaa tgccgtctga accttcagac ggcataccgt
     9181 acaaaccaat ctgccttaca cacactgagt ttgacatgca gttctcggca ttcggcgaaa
     9241 aattcacgca acacagcggc atcctgcaac tgatggacga cctcggcgac gcgctcaaaa
     9301 gcgacaagcc cgtcaatatg ctcggcggcg gcaacccggc gcgcattccc gaaatcgatc
     9361 gggcgttcgc cgacatattt tccaagctgg cggcggaaca cgccgtcgag aacatcggca
     9421 actactccaa tccccaaggc gatgccgcgc tgattgacgc gctgaccgcc ttccccaacc
     9481 gcgaatacgg ctggaacctg accgtcggca atatcgcgct gaccaacggt tcgcaaaacg
     9541 cgtttttcta tttattcaac ctcttcggcg gcaaattcaa gctttcagac ggcacatccg
     9601 cagaaaaagc cattttgttg ccgctcgcgc ccgaatacat cggctatgcc gacgtgcatg
     9661 tcgaagggcg gcacttcgtt tccgtcaaac ccaaaatcga aaacgtcgaa cacgaaggcg
     9721 aagcaggctt cttcaaatac cgcgtggact ttgacgcact ggaaaacctg cccgaactca
     9781 aagcgggcaa aatcggcgcg atttactgtt cgcgcccgac caacccgacc ggcaacgtgt
     9841 tgaccgacgg cgaaatggcg cgtttggacg ctttggcgcg tgaacacggc attccgccga
     9901 ttatcgacaa cgcctacgga atgccgttcc ccaacattat tgacagcggc gtaacgctga
     9961 attggcacga aaacatcatc ctctgcttca gcctttccaa agtcggcctg ccgggcgttc
    10021 gcaccggcat catcgtcgcc gcgcccgaag ccgtcaaagc cgtcagcagc ctgaacgcga
    10081 ttgtgaacct tgcccccacg cgcttcggcg cggccatcgc cgccccgctg ctggaaagcg
    10141 gcgagatgaa acggcttgcc gaccaagtca tccggccgtt ttaccgcaat caggcgcaaa
    10201 ccgccgtttc gctgctcaag cgcgagctgg gcgcgtaccc gatgaaaatc cacaaacccg
    10261 aaggcgcgat tttcctgtgg ctctggtttg aaaacctgcc cgtttcgtcg caaaccctgt
    10321 acgaaatgct caaagccgaa ggtacgctga ttattccggg cgaacatttc ttcgtcggca
    10381 tcgacacgca agactacccg cacgcgggcg agtgcatccg tatgagtatc gcacaggacg
    10441 ctcaaacgct ggaaaaaggc atcgccgcca tcggcaaaac cgtccgaaaa ccgtacgaca
    10501 acgtttaaaa cgcaaaaaat gccgtctgaa gacctttcag acggcatttt catccgcatt
    10561 caatgtcggg aaaaatgcgc ccgcaccggt ttgcagtttt ccggaagatc gggacacgcg
    10621 ccgaggatgc cgccgctgaa gtcgtctaaa cggtggaagt cgctgcccgc gcttgccaac
    10681 aatccgaagc ggtctgccaa aagcgcgtag ttgaggcggt cgtttttgca gcagttgccg
    10741 ctgtggactt cgatgcccgc gccgccgagg tttttaaatt cttcaaacag gttgcgcttg
    10801 gcggttgccg acaaatcata acgcatcgga tgggcaatca ccgccatgcc gcccgcgccg
    10861 ttgacggcgg agacgcagtc tgccagcgtc gcccattcgt ggcgtaccgc gcaggatttg
    10921 ccgtcgccca agtatttggt gaacgcctgc cgcttgtttt tgacgtgtcc gtttttgatg
    10981 aggaactcgg cgacgtgggt gcggctgacc atttctttgt ttgccgccag cgccagcgcg
    11041 ccgtcgtatg cgccgccgat gcctttcttt tcgagcttgg cggcgatggc ttcaagacgt
    11101 ttcagacggc ctttgcggac atctgccaac aggttttgca ggttttcgtc ctgctcgtcg
    11161 aaatccaaac cgacaacgtg tatggtacgc ccgcgccacg ttaccgaaat ttccacgccg
    11221 ttaatcaggc gcaaaccgag cctgtcggct tcggcacgcg cttcggagat gccgcccgtg
    11281 tggtcgtggt cggtcaacgc cagcagcgtg cagccgtttt gatgtgcgag gcggacgact
    11341 tcggcggggg ggagcatacc gtcggaaacg gtggaatggc agtgcaggtc tatcatgggg
    11401 tgttatgtgg tggtgaatga aggacgggat gtttcaatgt tgtcggacag tgtggcggcg
    11461 gcggtcattt taaaaaacag gtgtatggca aatgcaaagg aaaagccgct atgccgtcat
    11521 tcccgcgcag gcgagaatcc agactttgat ttattggaaa tgtttaaggt taaccgctat
    11581 ttcaaacttc cggattcctg ctttcgaggg aatggcgata tgaacgtttt caattttaat
    11641 ctactataaa agaccgtctg aaaacgtggt tttatcaagc ccaaccgttt tcagacggca
    11701 tcgtccggcc aacccgcttc cttcagtttt ttccgttgcg catcgtagcg tgccttgtcc
    11761 gcccagtaaa tcgtctgaat gcacgcctcg ccgcagtcgc tgccgcagca ttcccacgac
    11821 tcggggcgga cgggttcgtc caaaagcggc tcgcccaaaa gggcttcggc tttatgcttc
    11881 agggtcgtat ccatcggcga tttccaagcg cgcgccgtca aactcgatga cttctccgcc
    11941 gcgtattttg gccgttttgc ggatttcggt tccgccgttg cgcaacacca gcccttcggc
    12001 gataaatgct tttgcctgtc cgccgctttc ggcaagtccg gccaatttca agaggtcgca
    12061 caaggcgatg tattcgttgt cttcgagata gacggtggct tccataatgt ccccttttag
    12121 aaagagggcg ttattgtagc acctgccgcc gccgcaccca aaattgccgc aaaaccggcg
    12181 atgtgtccgc actgcctgtt ccgtaaaaat gccgtctgaa accccgtatg ccgccatccg
    12241 tccaaagaaa tcctgcccaa cggcaggctg caaatcctgt tccccgacga atccgcattg
    12301 acgctgatgc acatcctcaa acgcggactg cccgatacac cggcaatcgg catcaaaacg
    12361 aaatcaaaaa cctgtttgaa cgtaaaatct atttaactgc ttgccgtccg tccgaataag
    12421 gtatatagtt ccttataact attttgatag tcctttatat ctatcaatac tccttgggaa
    12481 gcctccgcca cacggcagga ggcatttttt tgccgtcgta aaagctcaaa aaacatttgc
    12541 aggtcatgcc gtctgaatcc gaaacggcat tacctgcacc gccatctaaa gacaaccctg
    12601 ctacaatacg ccttttattg tccacgccga ttttgccatg accgagccga cctacatccc
    12661 cctgcgcctg cataccgaat tctccattac cgacggtatg gtgcggatta aaaaactgat
    12721 tgccaaagcg caagaatacg gtttgcctgc tttgggcatc agcgatttga tgaacgaatt
    12781 cggtttggtg aaattctata aagcctgccg cggcgcaggg attaagcccg tcggcgcggc
    12841 ggacgtgtgg ataggcaatc cgaatgcgcc cgacaagccg ttccgcgcca tgctggttat
    12901 ccgcaacgat gcgggttatc tgcgcttgag cgagcttttg acggaggctt atgtcggcca
    12961 agaccgcaat atccatcatg cggaactcaa tcccgaatgg ctggaaaacg gcgacaacag
    13021 cggcttgatt tgtttgagcg gcgcgcatta cggcgaagtg ggcgtgaatc tgttgaacgg
    13081 caatgaagac gcggcgcgtg cggcggcgtt gaaatatgcg gcatggtttc ccgatacgtt
    13141 ttatttagag ctgcaacgcc tgcccgaacg ccccgaatgg gaggcttgcg tttccggcag
    13201 cgtgaagctg gcggaggaac tgggtttgcc ggtggtagca acgcatccga cacagtttat
    13261 gaaccgcgac gatttcaacg cgcacgaggc gcgggtgtgc atcgcgggcg gctgggtgct
    13321 gacggacaag aaacgtccgc gcgatttcac gccgagccag tttttcattc cgccggaaac
    13381 gatgctggag cgtttcgccg acttgcccga agccttggaa aacacggtgg aaatcgccaa
    13441 acgctgcaac ttgcacatca cattgggcaa aaacttccta ccccttttcc ccacgcccga
    13501 cggtttatca ctcgatgact gtctcatcaa actctccaac gagggtttgc aggaacgcat
    13561 ggttcagctt tatcccgacg aggcggaacg ggcggcaaaa atgccggaat atcaggagcg
    13621 gctggacttt gagctgaaca tcatcatcca aatgaaattc cccggctatt tccttatcgt
    13681 acaagacttt atcaactggg cgaaaacgca cggctgtccg gtcgggccgg gacgcggttc
    13741 gggcgcgggt tcgctggtgg cgtattcgct gaagattacc gacctcgacc cgctgaaata
    13801 cgcgctgctg ttcgagcgtt tcctaaaccc cgaacgcgtt tccatgcccg actttgacgt
    13861 ggacttctgt caggcaaacc gcggccgcgt gattgaatac gtgcgcgaaa aatacggcgc
    13921 tcaggcggtc agccagattg tgactttcgg cacgatgtcg tccaaagcgg tcatccgcga
    13981 cgtggggcgt gtgctggaac tgccgtttat gctgtgcgac aaactgtcca agctgattcc
    14041 gttggaagcc aacaaacccc tgagtttgga cgacgcgatg aaggcgcagc cgcagattca
    14101 ggaattgctt gaagcggaag aagcggacga actgattacg ctggcgaaaa aactggaaga
    14161 cctgacgcgc ggtttgggta tgcacgcagg cggcgtgttg attgcgccgg gcaagatttc
    14221 cgattacagc cccgtgtatc aggcggacga atccgcctcg cccgtatcca tgtacgacaa
    14281 gggcgacgtg gaagacgtgg gtttggtgaa gttcgacttt ttgggcctgc gcaacctgac
    14341 cattatcgaa atggcgcaga acaacatcaa aaacaccgcc ggcgatatcg tcgatgtcgg
    14401 caagattccg cttgacgacc agactgccta ccaaatcttc cgcgatgcga acaccaccgc
    14461 cgtcttccag ttcgagtcga ccggcatgaa aaaaatgctg aaaacggcgc atacgaccaa
    14521 gtttgaagaa ctcatcgcct tcgtatcgct ctaccgaccc ggcccgatgg acaacattcc
    14581 cgacttcgtc gcacgcatga agggacagga attccaatac atccacccgc tgctggaagg
    14641 catcctcgcg ccgacctacg gcattatggt gtatcaggaa caagtgatgc aggcggcgca
    14701 gattatcggc ggctactcgc tcggcggcgc ggacctgctg cgccgcgcca tgggtaagaa
    14761 aaagcccgaa gagatggtga aacaccgcga aatcttcgcc gaaggtgcgg caaaacaagg
    14821 catttcgcgc gaaaaatccg acgaaatctt caactacatg gaaaaattcg ccggctacgg
    14881 tttcaacaaa tcccacgccg ccgcctacgc cctgatttcc taccagaccg catggctcaa
    14941 agcccactat cccgccgaat ttatggcggc gacgatgtcg tccgaattgg acaacaccga
    15001 ccagctcaaa catttctacg acgactgccg cgccaacggc atcgagttcc tgccgcccga
    15061 cattaacgaa tccgactacc gcttcacgcc gtatccgaac atgaaaatcc gctacgcact
    15121 cggcgcgatt aaaggcacgg gcgaagccgc cgtcgaatcc atcatcgccg cgcggcaaag
    15181 cggcggcaaa tttaccggcc tgttggactt ctgcgagcgc gtcggcaaag aacacatgaa
    15241 ccgccgcacc ctcgaagccc tgatacgcgg cggcgcgttc gacagcatcg aacccaaccg
    15301 cgccatgctc ttggcgaaca tcgaccttgc catggacaac gccgaccaaa aagccgccaa
    15361 cgccaatcag ggcgggctgt ttgacatgat ggaagacgcc atcgaaccgg tgcggctcat
    15421 cgacgcaccc atgtggagcg aatcggaaaa actcgccgaa gaaaaaaccg tcatcggctt
    15481 ttacctgtcc ggccacccgt tcggcccgta tgcccaagaa gtccgccaaa tcgccccgac
    15541 caaattaggc cgtctgaaac cgcaagacag cgtgcgcctc gccggattcg ttaccgccgt
    15601 gcgcaccatg atgggcaaac gcggcaaaat cgccttcgtc agcctcgaag attcgagcgg
    15661 gcaggctgaa atcatggtcg gcggacaaac gctggaaaac tgcgccgact gcctcaaagc
    15721 cgaccaagtg ctgattatcg aatccaaagt cagccgcgac gattacggcg gcggcgacgg
    15781 gctgcgcatc atggcaaacc aagtcatgac cctgcaaacg gcgcgcgaac gctacgcccg
    15841 cagcctcagc ctcgcccttg ccccgcatca cgacatcggc ggactggtac ggctgctcgc
    15901 cgcccaccaa ctgcccgaca cgccgcgcat cccgcttcaa ttgtcgtatg ccaacgaaaa
    15961 agcgtcgggc aggcttcaag tgcctccgaa atggacggtt acaccgagtt ccgcattgtt
    16021 cggcgaactg gaaacattgc tcggcagccg gtcggtgcgc gtgaattggt aaccaaaaat
    16081 acaaatgccg tctgaagccc aaaaccggtt tcattcgtac tttattcgaa tgattgaata
    16141 aaagtaactg ccaagaaaaa cgtatttttt ggttatttcg ccagcctaaa taaatcaacc
    16201 ggggcgattg atatccgtgt acatgacaca gacagcacca aagggaaaaa cggcattttc
    16261 aaaagtatcg gtgtcaaaac cgccctttca ttccaaaaat accaaatcga cgaaccgggc
    16321 aaagaaatca gaccgtgccg cctgaaaaaa cgcccgcccg tccgttaaat taatctatcc
    16381 ctgtttcagg cggcctgaag cagggatttt tatatcaaga taaaatgaga acgggagcga
    16441 caaccacggg caaagtcggc gcgaactatc aaaaattgta gcactgcgaa atgaggacgg
    16501 gagcaataac ccttaggtag ctcttgttat agcggattaa cgaaaatcag gacaaggcga
    16561 cgaagccgca gacagtacaa atagtacgga accgattcac ttggtgcttg ggtgccttag
    16621 ggaatcgttc tctttgagct aaggcggggc aacgccgtac cggtttttgt taatccgcta
    16681 tattttccga tgcaaaacaa agcagtcata tatttgattc cccctaccgt tgccacgctt
    16741 ccctcaaata ttcgacgcag tcggtcagcg agtaaaacgg gacattgccg tggtcggcat
    16801 tcggatattc ccggaaaaat acggcggtcc cgcgcctgtc caactctgcc gccatttgtt
    16861 cggcctgccc tgccatgtcg cgttcttccc tgcgtttgca accgctgctc cgctcgagtg
    16921 cgccgatgtt gaggcaggca tcgatgccgt ccagccggtt ttcagacggc ataaagtcga
    16981 gtatccgcct gttgtgccac caaatcgagg ggatacgagc caatgccgtc tgaaacagcg
    17041 gtgggaaagc agggaataca gtccgaacag tgcgccgaac gagtgtccga atacggcggt
    17101 ttcattacgg ttaagggtgt agcggctttc taaaaaggcg gtcagttcgc tgtcgataaa
    17161 gtcggcgaag cggtctgcct gtccgaactg ccgccgttcg tctgctgtgg cgttgtctcc
    17221 aagcggcggc gtgtagtcgg cggcacgctg cgccaggtcg cgcacactgc ctgtcgtgta
    17281 gccgataccg acaatcaggc agggggcgtt gcttcgggta acggggttgt tcatcagcga
    17341 ctgcatgatg ttgtacagcg cgggaaaaag gcttcgccgt cgaggacaaa gaggacggga
    17401 tagccttcag actgcatttc gccgagtgtt gccgtctgaa tccgatagat tcgccccgtg
    17461 caggtggatt tgatttcggt ttcaaaggct tggggcagta tggcaggttg gaatgtgtcg
    17521 gtcggtatgg gtttcatgat gttcggcttg tgggtcagac tggcggcaag cggtaaaacc
    17581 gaaccggcaa agcggtctgc ctgccggttc ggtctgtttt ccttcgcaat gccatactcc
    17641 ggttgtgagc gcatagggta tgccgcgcag cttgttgtgg tttgattatg ctgcggctgc
    17701 cgtttcagac ggcatattgg tctttaaaaa ctgtaacgca ggtttgccgt caggctgcgc
    17761 tccgaaccgg gaatgttaaa ggtgttcgtg ccgccgacgc gggcgtagta atggcggttg
    17821 aagatgttgt cggcattgat ttgcagcttc agcttgggcg tgaagcggta tgccgccatc
    17881 gcatcgaacg tggcataacc gcctgcatgc atccctgcag atgaagtaat gccgctcatc
    17941 gcgttcacgc cgccgccgac ggtcagcccg ggcgtaactt ggtaagtcgt ccacaggttt
    18001 gcgctgtgtt tgggcaccag caggaagatg ccgtcgtcgc gtggattggc ggcggttttg
    18061 atttggctgt gcagatagct gtaacctgca tggatttgcc atttcggtgt aatcgcaccg
    18121 ctgatttcgg tctcaacgcc ttccatcacg cgtttgccca atgcggcgta acgggtgttg
    18181 cggttgttcg ggttcggcgg cgtttttgtc tttcaggcgg cagggggaga cgcgggtatt
    18241 gaggcggtca tccatatagc tgcctttaac gagctgtaat cgccgttttt ctcggtggca
    18301 atcgcgttgt acgaacgctg ccctttgata tggacggttt ccaaatccgc cgtttgtgcg
    18361 gcaaaaaccg gagacgagag tgctgccaaa accgtggcgg cggtcatatt gatgcggaaa
    18421 actgacataa actgtcccat ctgtataaat gataatggtt ctattttaat aaagcacaac
    18481 gcggcttgtt cggaaaaaca tatcgctcag ccgacaaatt ttgtcgaaaa tgcgacacgt
    18541 ctgcgttttc cgcataaaat ctgctttttt actgcaacca acctgccatg accccgccca
    18601 aactcatcat cttcgactgg gacggcacgc ttgccgatac gacccaaccc atcatcgaca
    18661 ccatgcgccg cagcttcgcc gaatgcggtt ttccgccgcc cgaagcggag cgcgtccgca
    18721 gcctgatcgg atacagcctg cccgaaatca tccgcgccct gctcgaaatg ccttctgaag
    18781 ctgccgttgc cgacatcgcg cgcacttatt ccgcgcatta cctcaatccc aacaaccgca
    18841 atatgacact ctttcccgat gccccgccct gtctggacaa gctcaaagcg caaggcttcc
    18901 ggcttgccgt cgccacgggc aaagggcggg cgggtttgga caacgccatc agccaaaccg
    18961 ccaccggcgg ctattggctg gccaccgcct gcgcggggga atacccctcc aaaccctcgc
    19021 ccgaaatggt attcggactc tgcggcgaac tgggactcga cccgaaagag gcattggttg
    19081 tcggcgatac ggcgcacgac ctgcatatgg cggcaaacgc aggcgcggcg gcagtcggcg
    19141 ttgccaccgg cgcacattcg cgcgaacagc tccttggcgc accgcatctc gccgtattgg
    19201 acggtttgtc cgaactgccc ggttttcttg cacgacatta cgcctgattg gtttccgcat
    19261 ccggcacgcg gcaaaaatgc cgtctgaagc ctgttcagac ggcatgag
//
LOCUS       DAWTHD010000027        18841 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWTHD010000027 DAWTHD010000000
VERSION     DAWTHD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18841
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.027"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(82..1305)
                     /locus_tag="WM704_000762"
     CDS             complement(82..1305)
                     /locus_tag="WM704_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFA9340228.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            1742..2155
                     /locus_tag="WM704_000763"
     CDS             1742..2155
                     /locus_tag="WM704_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9340229.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            2202..2831
                     /gene="nth"
                     /locus_tag="WM704_000764"
     CDS             2202..2831
                     /gene="nth"
                     /locus_tag="WM704_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFA9340230.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            2969..4468
                     /locus_tag="WM704_000765"
     CDS             2969..4468
                     /locus_tag="WM704_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFA9340231.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            4671..6191
                     /locus_tag="WM704_000766"
     CDS             4671..6191
                     /locus_tag="WM704_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFA9340232.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRADV"
     gene            6248..7333
                     /gene="nagZ"
                     /locus_tag="WM704_000767"
     CDS             6248..7333
                     /gene="nagZ"
                     /locus_tag="WM704_000767"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFA9340233.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDSEGAET
                     AETHAEHIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            7558..7634
                     /locus_tag="WM704_000768"
     tRNA            7558..7634
                     /locus_tag="WM704_000768"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7592..7594,aa:Pro,seq:cgg)
     gene            complement(7970..8608)
                     /locus_tag="WM704_000769"
     CDS             complement(7970..8608)
                     /locus_tag="WM704_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFA9340234.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(8605..8973)
                     /locus_tag="WM704_000770"
     CDS             complement(8605..8973)
                     /locus_tag="WM704_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFA9340235.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
     gene            complement(9244..9678)
                     /gene="queD"
                     /locus_tag="WM704_000771"
     CDS             complement(9244..9678)
                     /gene="queD"
                     /locus_tag="WM704_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFA9340236.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(9766..10281)
                     /locus_tag="WM704_000772"
     CDS             complement(9766..10281)
                     /locus_tag="WM704_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFA9340237.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(10309..10968)
                     /gene="queC"
                     /locus_tag="WM704_000773"
     CDS             complement(10309..10968)
                     /gene="queC"
                     /locus_tag="WM704_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFA9340238.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(11153..11358)
                     /locus_tag="WM704_000774"
                     /pseudo
     CDS             complement(11153..11358)
                     /locus_tag="WM704_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            11457..12683
                     /locus_tag="WM704_000775"
     CDS             11457..12683
                     /locus_tag="WM704_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFA9340239.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(12704..12985)
                     /locus_tag="WM704_000776"
     CDS             complement(12704..12985)
                     /locus_tag="WM704_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9340240.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            13064..13180
                     /locus_tag="WM704_000777"
                     /pseudo
     CDS             13064..13180
                     /locus_tag="WM704_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13178..14899)
                     /locus_tag="WM704_000778"
     CDS             complement(13178..14899)
                     /locus_tag="WM704_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFA9340241.1"
                     /translation="MDYLQNLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDL
                     RALRSKYCLSLKGENLADIVRFADDMGLTGRALRLDLDELGSLRLPCILHWDLNHFVV
                     LESVSSDGAAVMDPASGRRKVKTEEISRKFTGIALELWPNTRFEAGEEKQEIRILPML
                     RGISGLGRTLFQLLALAAAMEVFALVSPFFMQWVIDHVVVTGDRNLLATLALGFTLLL
                     LVQNTVSVMQGWLGMHFSTTLNVQWKSNVFKRLIDLPTDYFAKRHLGDVVSRFGAVDS
                     IQGTLTSTFFVLVLNSLTAVFTFVLMIVYSPILTGLVAATLVVYIAVRWVAYYPLRRA
                     TEENIIHAAKQSSYFMETVRGIRAVKQFGKGPQRYSAWMGLLVDTVNTGLTVQKLGIW
                     FGLANKLLFGLANILIIYLAAGMVLDGVFTVGVLMAFLAYKGQFEGRIGSLVDQFVQI
                     KMLSLHAERLADTVLNETESEATPDVGIPDISDDIEISVENVSFRYADNEPYVLQNVS
                     FKIGRGESLALIGRSGCGKSTFLDILSGNLPPESGKVMINGHDIYSLPPRFIRNLSAM
                     VRQDDVL"
     gene            complement(15001..15216)
                     /locus_tag="WM704_000779"
     CDS             complement(15001..15216)
                     /locus_tag="WM704_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340242.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(15230..15577)
                     /locus_tag="WM704_000780"
     CDS             complement(15230..15577)
                     /locus_tag="WM704_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340243.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(15762..17036)
                     /locus_tag="WM704_000781"
     CDS             complement(15762..17036)
                     /locus_tag="WM704_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFA9340244.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLFLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(17097..17451)
                     /locus_tag="WM704_000782"
                     /pseudo
     CDS             complement(17097..17451)
                     /locus_tag="WM704_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17444..17671)
                     /locus_tag="WM704_000783"
     CDS             complement(17444..17671)
                     /locus_tag="WM704_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340245.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(17680..18834)
                     /locus_tag="WM704_000784"
     CDS             complement(17680..18834)
                     /locus_tag="WM704_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFA9340246.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
BASE COUNT         4572 a         5067 c         4677 g         4525 t
ORIGIN      
        1 acctcggcac acacgccgca aacaccggta cgcaaaaatg ccgtctgaaa gatgttcaga
       61 cggcatttct caagaaaaag attatttgtc cgccctatat ccgtacaccg caaaaaacac
      121 gatatacagg taacagataa cggaaacgac aaacgaagac atcagggtgt aagtatccgc
      181 cgcccaacct tgaattaccg gcacgaccgc accgccgaca atcgcggtac acagcacacc
      241 ggaagccgcg ttggtgaatt tgcccaaccc tttggttgcc aaagagaaaa tcgtcgggaa
      301 cataatcgag ttgaaaaaac cgatggcaag cagcgaccac atcgccacat ccgcattgcc
      361 gctgcccgtc gccatcgcga cggcaagcag cacgaccgca gccgatgcgt taaacgccaa
      421 ataacggttg ggcgcgaatt tcgccattac cgccgaaccg aggaaacgtc cgaccatcgc
      481 accgccccaa taaaacgaca ggtaatgcgc ggcagaagca tgatccagcc ctttcagata
      541 acccaacaca ttgaccatca gcgaaccgat agacacctcc gcaccgacgt agcagaaaat
      601 gcctgccgta ccgaacacga gatgtttgta ttgccatacg ccggttttgc cgtcgtggtt
      661 gtgcgcgctt tcctcggcgg caattttgcg cgcgtcgggc agccggatca ttttcacgaa
      721 aacggcaagg ataatcagca gccccgccag tcccaaatag ggaatctgta cggaagaaat
      781 ctgttccgcc ttgctgacgg tttgggttgc gtccgccaaa atcaggaacg cgccgatttg
      841 aggcgcaatg gtcgtaccca aagcgttaaa cgcctgaacc agcgtcagcg ttgccgattc
      901 cttgccgggt ttcgccagca gggtaacata aggattaccg gcgacctgaa gcagcgttac
      961 gccggaagcc aaaataaaca acgcgcccaa aaataccgcg taagaatggc tgcccgcagc
     1021 aggataaaac agcaggcacc cgaccgccgt cagcagaaag ccgccgataa cgccgttttt
     1081 gtagccgact ttgccgacaa aagcccccat cgggatggac attaccgcat aggcggtaaa
     1141 aaagcaaaat tgaatcagca tcgcctgaac gtaagagagg tcgaaaattt ctttcaaatg
     1201 agggataagg atgtcgttca tgcaggtaat aaaacccatc atgaagaaca gcgtggtcaa
     1261 gacgaccaat ggggacgtat ggttgttttg tgattgcgca gacatgattt gcctttcgtg
     1321 gtgtcggaat attgcccgtt tcaagaaaat agaaattgac atttcttaac ggcaatgcaa
     1381 atcatactga tatttgaaca ttttgcaaac tttatcggat gcaggcaatg cgccgatctg
     1441 aaattcaagc agcagaaatt aatattttac agttgtttgc ataggtttta atagatttta
     1501 aaaaattccg gcgggcttgc aaaaaccgtt cccaataaaa tgaaaatcct ccgccgtatc
     1561 cggaaaattc ggaaatgccg catcggggca tcaaaaccgg agcggaaaga atgtcgtatg
     1621 cccgacaaat aaaggggcat acggctgtgc cgtatttttg caccgtcctc cgcccttcgg
     1681 cgggggcgtg aaaataccgt ataatccgtc gccgattttt acctgtttgg gactgtttgc
     1741 aatgataccc tcctacacct acgccgccct cgctttttcc gccttcactt ccgccaccct
     1801 cctgcccggc acgtccgaag ccgccttcgc cctgttcgtc cgcaacttcc ccaaacacgc
     1861 atacggcgcg ttgctgtgcg ccggccttgc caacggattg ggcagtatgg tttcctattg
     1921 gatgggacgt ttgctgcctt cccgaaaaat gccgtctgaa aaaacactga atctgatacg
     1981 gcgtttcggt atttggctgc ttgcgtttgc gtggctgccc gtcgtcggcg acgcactgcc
     2041 gcttaccgcc ggctggctgc ggttgaatcc gtggacaagc gggctgatgc tggttatcgg
     2101 caaaacggcg cgttacgcct tcattctgtg gggaatgcaa tattacgccg cctgaaccgt
     2161 gcgttataat gccgaccgca caaaccgact gcacaaaaaa aatgaacaga caaatccgcc
     2221 aagaaatctt cgagcgtttc cgcgccgcca acccccatcc gaccaccgag ctgaatttca
     2281 actccccttt cgagctttta atcgccgttc tgctttcggc gcaggcgacc gatgtcggcg
     2341 taaacaaggc gacggcgaag ctgtttccgg ttgccgatac gccgcaggcg atgctggatt
     2401 tgggcttgga cggcgtgatg gaatacacga aaaccatcgg gctgtataaa accaagtcca
     2461 aacacattat gcaaacctgc cgcatcctgc tggaaaaata caacggcgaa gtgccggaag
     2521 accgcgaggc tttggaatcg ttgcctggcg tggggcgcaa aacggcaaac gtggtattga
     2581 acacagcgtt cggacacccc gttatggcgg tcgatacgca tattttccgc gtgtccaacc
     2641 gaaccaaaat cgcacccgga aaagatgtac gcgaagtcga agacaaactg atgcgcttca
     2701 ttcctaaaga atttctgatg gacgcgcacc actggctgat tttgcacgga cgctacacct
     2761 gcaaggcttt aaagccgcaa tgccaaacct gcatcatcaa cgatttgtgc gaatatcccg
     2821 ccaaatcctg accgccgccg cccttcaaaa tgaaacgctt gaaaattcgg acggacgttg
     2881 tagaatttca acctatgccg tctgaagccg acaaaatgcg cttcagacgg cattaaagcc
     2941 ggtaccggca tccgacaaag gacaaaacgt gttcaaaaaa taccaatact tcgctttggc
     3001 ggcactgtgt gccgccttgc tggcaggctg cgaaaaggca ggcagctttt tcggtgcgga
     3061 caaaaaagaa gcatccttcg tagaacgcat cgaacacacc aaagacgacg gcagtgtcag
     3121 tatgctgctg cccgactttg cccaactggt tcaaagcgaa ggcccggcag tcgtcaatat
     3181 tcaggcagcc cccgccccgc gcacccaaaa cggcagcggc aatgccgaaa ccgattccga
     3241 cccgcttgcc gacagcgacc cgttctacga atttttcaaa cgcctcgtcc cgaacatgcc
     3301 cgaaatcccc caagaagaag cagatgacgg cggattgaac ttcggttcgg gcttcatcat
     3361 cagcaaaaac ggctacatcc tgaccaatac ccacgtcgtt gccggtatgg gcagtatcaa
     3421 agtcctgctc aacgacaagc gcgaatatac cgccaaactc atcggttcgg atgtccaatc
     3481 cgatgtcgcc cttctgaaaa tcgacgcaac ggaagagcta cccgtcgtca aaatcggcaa
     3541 tcccaaaaat ttgaaaccgg gcgaatgggt cgctgccatc ggcgcgccct tcggctttga
     3601 caacagcgtg accgccggca tcgtgtccgc caaaggcaga agcctgccca acgaaagcta
     3661 cacacccttc atccaaaccg acgttgccat caatccgggc aattccggcg gcccgctgtt
     3721 caacttaaaa ggacaggtcg tcggcatcaa ttcgcaaata tacagccgca gcggcggatt
     3781 catgggcatc tcctttgcca tcccgattga cgttgccatg aatgtcgccg aacagctgaa
     3841 aaacaccggc aaagtccaac gcggacaact gggcgtgatt attcaggaag tatcctacgg
     3901 tttggcacag tcgttcggtc tggataaagc cagcggcgca ttgattgcca aaatccttcc
     3961 cggcagcccc gcagaacgtg ccggcctgca ggcgggcgac atcgtcctca gcctcgacgg
     4021 cggagaaata cgttcttccg gcgaccttcc cgtcatggtc ggcgccatta cgccgggaaa
     4081 agaagtcagc ctcggcgtat ggcgcaaagg cgaagaaatc acaatcaaag ccaagctggg
     4141 caacgccgcc gagcataccg gcgcatcatc caaaacagat gaagccccct acaccgaaca
     4201 gcaatccggt acgttctcgg tcgaatccgc aggcattacc cttcagacac ataccgacag
     4261 cagcggcaaa cacctcgtcg tcgtacgggt ttccgacgcg gcagaacgcg caggcttaag
     4321 gcgcggcgac gaaatcctcg ccgtcgggca agtccccgtc aatgacgaag ccggtttccg
     4381 caaagctatg gacaaggcag gcaaaaacgt ccccctgctg gtcatgcgcc gtggcaacac
     4441 gctgttcatc gcattaaacc tgcaataacg cacataattt aaccaaaata tttttaaatg
     4501 ataaaatgcc cgtcaatacc gattaacggg cattttacaa aaatcagttg gttgatttta
     4561 aacaaatatt ttttacgaca aaaaatattt ccacccattt tgccaacaaa acccaaaccg
     4621 gaggcggaca gatacccacc catccggacg cttaacaccc cccataaacc atgcagctga
     4681 ttgactattc acattcattt ttctcggttg tgccaccctt tttggcactg gcacttgccg
     4741 tcattacccg ccgcgtactg ctgtctttag gcatcggtat tttggtcggc gttgcctttt
     4801 tggtcggcgg caaccccgtc gacggtctga cacacctgaa agacatggtc gtcggcttgg
     4861 cttgggcaga cggcgattgg tcgctgggca aaccaaaaat cttggttttc ctgatacttt
     4921 tgggcatttt cacttcactg ctgacctact ccggcagcaa tcaggcgttt gccgactggg
     4981 caaaacggca cattaaaaac cggcgcggcg cgaaaatgct gaccgcctgc ctcgtgttcg
     5041 taacctttat cgacgactat ttccacagcc tcgccgtcgg tgcgattgcc cgccccgtta
     5101 ccgacaagtt taaagtttcc cgcgccaaac tcgcctacat cctcgactcc actgcctcgc
     5161 ccatgtgcgt gctgatgccc gtttcaagct ggggcgcgtc gattatcgcc acgcttgccg
     5221 gattgctcgt tacctacaaa attaccgaat acacgccgat ggggacgttt gtcgccatga
     5281 gcctgatgaa ctattacgcg ctgtttgccc tgattatggt attcgtcgtc gcatggttct
     5341 ccttcgacat cggctcgatg gcgcgtttcg aacaggctgc gttgaacgaa gcccacgacg
     5401 aaaccgccgc ttcagacgct accaaaggtc gtgtttacgc attgattatt cccgttttgg
     5461 ccttaatcgc ctcaacggtt tccgccatga tctacaccgg cgcgcaggca agcgaaacct
     5521 tcagcatttt gggggcattt gaaaacacgg acgtaaacac ttcgctggta ttcggcggca
     5581 cttgcggcgt gcttgccgtc gtcctctgca cgttcggcac gattaaaacc gccgattatc
     5641 ccaaagccgt gtggcagggt gcgaaatcca tgttcggcgc aatcgccatt ttaatccttg
     5701 cctggctcat cagtacggtt gtcggcgaaa tgcacacggg cgactacctc tccacgctgg
     5761 ttgcgggcaa catccatccc ggcttcctgc ccgtcatcct cttcctgctc gccagcgtga
     5821 tggcgtttgc cacaggcaca agctggggga cgttcggcat tatgctgccg attgccgccg
     5881 ccatggcggt caaagtcgaa cccgcgctga ttatcccgtg tatgtccgca gtaatggcgg
     5941 gggcggtatg cggcgaccac tgttcgccca tctccgacac gaccatcctg tcgtccaccg
     6001 gcgcgcgctg caaccacatc gaccacgtta cctcgcaact gccttatgcc ctgacggttg
     6061 ccgccgccgc cgcatcgggc tacctcgcat tgggtctgac aaaatccgcg ctgttgggct
     6121 ttggcacgac cggtattgta ttggcggtgc tgatttttct gttgaaagat aaaaaacgcg
     6181 ccgacgtttg accttccccc ctgttcagac ggcatatgcc gtctgaagtt ttttggagca
     6241 aaccgcaatg accgtccccc atattccgcg cggccccgtc atggcagaca ttgccgcctt
     6301 ccgcctgacc gaagaggaaa agcaacgcct gctcgatccc gcaatcggcg gcatcatcct
     6361 cttccgccgc aacttccaaa acatcgaaca actcaaaaca ctcaccgccg aaatcaaagc
     6421 cctgcgcaca cccgaactca ttatcgccgt cgatcacgaa ggcggcaggg tgcaacggtt
     6481 tatcgaaggt tttacccgcc tgcccgcaat gagtacgctc ggcgagattt gggacagtga
     6541 aggcgcggaa actgccgaaa ctcatgccga acacatcgga tgggttttgg caaccgagct
     6601 ttccgcctgc ggcatcgatt tgtccttcac gcccgtctta gatttggact ggggaaactg
     6661 cgccgtcatc ggcaaccgca gcttccaccg caatcccgaa gcggtcgccc gcctcgccct
     6721 cgcccttcaa aaaggtttgg caaaaggcgg tatgaaatca tgcggcaaac atttccccgg
     6781 acacggattt gtcgaagggg acagccattt ggtgctaccc gaagacgggc gcagcctcga
     6841 cgaactcgaa gccgccgacc tcgccccctt ccgcattatg agccgcgaag gtatggcggc
     6901 ggtcatgccc gcccacgtcg tttatccgca agtggacaca aagcccgcag ggttttccga
     6961 aatctggctc aaacaaattt tgcgccgcga tatcggattc aaaggcgtga ttttttctga
     7021 cgatttgacc atggagggcg cgtgcggggc aggcggcatc aaagaacgcg cccgcatttc
     7081 ctttgaggca ggttgcgaca tcatattggt ctgcaaccgt cccgacttgg tcgatgaatt
     7141 gcgcgacggt ttcaccattc ccgacaacca ggatttggcc ggacgttggc aatacatgga
     7201 aaactcgctc ggtcatgagg cggtacaagc cgttatacag acaacgggtt tccaagcggc
     7261 gcaagccttt gttgccggcc ttgcctcgcc gcaggacacg gcgggcggcg taaaagtcgg
     7321 agaggccttt taaaccggct gcgtttcaac aatctgccga tttcaaccgt tcgtcaggga
     7381 aaggcaatga ttttttcggt caaatagaaa gttatcttat tgaattactt tgagataagg
     7441 atgcttatca atactgtatt caaacacagc cctttaaatc cgcccgacac gcggcaaaaa
     7501 ggcaaaacgg tataggcacg ccgtattttt tgcgctataa tcacacctta attaattcgg
     7561 aatgtagcgc agcccggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc
     7621 tctcattccg accatattca gaccgcctcg acatcgattg ccggggcggt ttttcccttt
     7681 tggcggcaac aaaaaaatgc cgtctgaaac agaatgctgt ttcagacggc atttcgtttt
     7741 ccccaatcct gccatttgcg cgcactgaca aagggtatcg gcaaggttca aaagaccatc
     7801 ggaatctagg aagcctggcg tgtacccaac cgcggcatct tattcattaa ggcttcgtcc
     7861 tgattgggcg gcggaaatgt cggcagtttc acgcggaagc ggcacacttt ccccgtatgt
     7921 caaacatcgg caagaaggta cgcctgccct ttgggttaca ctgcttaaac tactctatcc
     7981 ccgcccattt gtgcgtctgc acgctcaact gccaatgcac gggcgcgtcg gggcggctgt
     8041 ttaaaatacc gatttggcgg atggtgtcgt agatgttcat cacgccgttt tgttcgcagg
     8101 gagaaagata gtaatggcgc gcacggattt tgcgttccat attttcgcaa aacgcaagga
     8161 catcgccgtc ggcaacaatc cgtacttcgt cggcttcggc gatgcagttg gtttcatact
     8221 tggcggcgta gcaggctttg gggctggtgg cgacatagtc gatttgcggc ggcgcgggct
     8281 tgagcccgtt ggtttcgaga cagaggaaat agccttccgc cttgagcgtg tccagcagca
     8341 tatcgagatg cggctggatg gtcggctctc cgccggtgat gatgatgttg cgggcggcat
     8401 aagttttcag acagcctaag atgtcggaca ggctcatcat accaaatgtc aaataatcgg
     8461 tatcacacca gccgcacgcc agattgcatt tgcccaagcg gacgaaaacg gcaggcatac
     8521 ccgtgtttcg gccctcacct tgcaggcttt caaagatttc gacgatgcgg tactggggat
     8581 tttcgggggc gacgctgatt tttttcatac cgccaacacc gccgccgcca aaaatgcggg
     8641 caaaccttgt ttgacgagta tgcctttgtt gccggacgaa aacgcacccc acgcggcggc
     8701 aatcaggacg aagccgagaa acagtaccgt cgcgccgtac acggcattat ccggagcggc
     8761 aaactgcgcc cacaaaagcc cgacggcaag aaagccgttg tacagccctt ggttgccaaa
     8821 cagggtctgc acgcgtttct gttccataaa ttcgtaaggc agcttgaaca tttccgccgc
     8881 tttttcgccg ggaatctgcg tcatttcaag ccaggcgatg tagaaatgtt cggcggcgac
     8941 gaggaggacg agaatggtgg agaggagttt catgggtgtt cctttcaatg ggtgtgccgg
     9001 tatgaaatgc cgtctgaaaa aacggggaat ccgtgcagga gaaaaaaccg ctttacggta
     9061 tagtggatta acaaaaatca ggacaaggcg gcgaagccgc agacagtaca gatagtacgg
     9121 aaccgattca cttggtgctt cagcacctta gaaaatcgtt ctctttgatc tgaggcgagg
     9181 caacgccgta ccggtttaaa gttaatccac cataccttat ttgaattgac tatatcgata
     9241 cgttcaaaat attccctccc ccccttcata ctccgcgcac gatgtcggcg tttcccacaa
     9301 tttgacgcgg cacacgttca gccccgcgtt tttcagacgg cagtacattt cgaccgccat
     9361 attttcggca gttgtgcggc agggcaggcg cagggttttc atgttccagc cttccaaaag
     9421 cgcggcgatt tgagattcgc gcccgttgcc gccgtggtag atgaaggcgt ggtcgaaatg
     9481 gtcggtaatg tgttgtttca caatggcttt caagtcggta aagtccatca ccataccgtc
     9541 tttcgctccg cctttgatgg tgccgtctga aacggtgatt tcgagtttgt aggtatgtcc
     9601 gtgcaggttt tggcatttgc cgtcatgccc gtcgagcata tgcgaggagt cgaaggtaaa
     9661 tatcttggta attttcatcc cgattccttt tcagtgaaaa ccccgccgct ttccgcccgg
     9721 agggtggctt gatgtgtcgt tttgcgtgga aacatatccg ctgcttcaaa tgggcaaata
     9781 gccttgaaag ccgatttcgg cgggtttgcc gtgcggattc ggggtcaggc ggatatggaa
     9841 attgccgatg tgctcgagcg caagcaggtt gtccacgtct ttcaagttgt ctttatgctg
     9901 ccgaacatag tcggtcaaca gggtttgcag ggctttttgt ttggcttcgg caggcgtggc
     9961 ggcggcgaac agcccgaacg cgtgcgcctc ggcaagctgc cccgcgcgat agccgccgac
    10021 attggcaaaa tacaggcgcg gagcgttttc aggcggcatt ttcgttttgg gcgtttcgga
    10081 tacggcgatg tcgtaaccgt ccgcccattc gacaacctgc caaccgtcga tgtggatttt
    10141 gtccgtatcg ccgaaccacg cggctttgag tgcggggacg gcttcgcggt agtcgtcgca
    10201 cacggcaaat tggatgtcgt gcacttcgat attcgaccgg ccggcattgc cgccgaggta
    10261 aaacatatgg agcttgggca tatcgtattc cttgtgcttt gcgcgcgttc agacggcctt
    10321 tttactttcc agacattccg ccagcccgcg ttcgcgcaag atacagctcg ggcattcgcg
    10381 gcagccgccg acgataccct tgtagcaggt gtgggtctgt tcgcggatgt agtccaacac
    10441 gcccatttcg tccgccaacg cccacgtttg cgccttggtc aggtacatca gcggcgtgtg
    10501 gatttgaaaa tcgtagtcca tcgccagatt aagggtaacg ttcatggatt tgacgaacac
    10561 gccgcggcag tcgggatagc ccgaaaaatc ggtttcgcac acgcccgcga tgatgtgccg
    10621 gataccctgc cctttggcaa aaatggcggc gtaaagcagg aaaagcgcgt tacgcccgtc
    10681 cacaaaggta ttgggaacgc cgttgtcggc ggtttcgatg gcggcggttt cgtccatcag
    10741 ggcgttgtgc gtaatctgcc gcatcaggct caaatcgagt acggtttgac tgacacccaa
    10801 atcctgcgcg atccactctg cgcgttccag ctcgacggca tggcgttgcc cgtatcggaa
    10861 ggtgatggct tggacgtttt cgcgcccgta ggtttggatt gcctgaatca ggcaggtggt
    10921 cgaatcctga ccgcccgaga agatgaccaa ggctttttgg tttgacatat cgaatcctag
    10981 ttttggtaac gcgggaggtt tgcgaaccgc gaagttggac agtaaagacg cggattatac
    11041 aggcaaacgc gttcagacgg catctgaacg ccatgccgtc tgaacacgcg gcgtatgccg
    11101 acttgacggc gagttccgcc aagcgtctgc cttgtgcgaa ggcgatattg ttttcttcgg
    11161 cggtcaggac gggagccgct gtcgggaata tctttatttg acggcttcgc agacggcgga
    11221 cactttgggg acggtgcgca atacggcttc gcaaccttca acgctgtcga tgccgcgagt
    11281 gattcggcgt gcgggattgc gggtgctgcc gttttgggaa tagtagagga cgaggatttt
    11341 gaggggattt gggttcatgg gcggattccg ttaaaatagt ttggtgttca gacggcataa
    11401 cggttgtcca gatgccgtct gaaacagttt gaaaacatga aacctggtaa gagattatga
    11461 cctttttaca acgttggcaa ggtttggcgg acaataaaat ctgtgcattt gcatggttcg
    11521 tcatccgccg tttcagtgaa gagcgcgtac cgcaggcagc ggcgagcatg acgtttacga
    11581 cactgctggc actcgtcccc gtactgaccg taatggtcgc ggtcgcttcg attttccccg
    11641 tgttcgaccg ctggtcggat tcgttcgtct ccttcgtcaa ccaaaccatt gtgccgcagg
    11701 gcgcggatat ggtgttcgac tatatcgacg cattccgcga tcaggcaaac cggctgaccg
    11761 ccatcggcag cgtgatgctg gtcgtaacct cgctgatgct gattcggacg atagacaatg
    11821 cgttcaaccg catctggcgg gttaacacgc aacgcccctg gatgatgcag ttcctcgttt
    11881 attgggcgtt gctgactttc gggcctttgt ctttgggtgt gggcatttcc tttatggtcg
    11941 ggtcggttca agactccgta ctctcctccg gagcgcaaca atgggcggac gcgttgaaga
    12001 cggcggcaag gctggctttc atgacgcttt tgctgtgggg gctgtaccgc ttcgtgccca
    12061 accgcttcgt gcccgcccgg caggcgtttg tcggagcttt gattacggca ttctgcctgg
    12121 agacggcacg tttcctgttc acctggtata tgggcaattt cgacggctac cgctcgattt
    12181 acggcgcgtt tgccgccgtg ccgtttttcc tgctgtggtt aaacctgctg tggacgctgg
    12241 tcttgggcgg ggcggtgctg acttcgtcgc tgtcttattg gcagggcgag gccttccgca
    12301 ggggattcga ctcgcgcgga cggtttgacg acgtgttgaa aatcctgctg cttctggatg
    12361 cggcgcaaaa agaaggccga accctgtccg ttcaggagtt cagacggcat atcaatatgg
    12421 gttacgatga attgggcgag cttttggaaa agctggcgcg gtacggctat atctattccg
    12481 gcagacaggg ctgggttttg aaaacggggg cggattcgat tgagttgagc gaactcttca
    12541 agctcttcgt gtaccgcccg ttgcctgtgg aaagggatca tgtgaaccaa gctgtcgatg
    12601 cggtaatgac gccgtgtttg cagactttga acatgacgct ggcggagttt gacgctcagg
    12661 cgaaaaaaca gcagcagtct tgagtatttt tgaaactgta tttttatcct aaagacattt
    12721 tcctatctgc tgattccacc gtttcctttc tgtgtgccgc cataattttt ataatagaca
    12781 agccattcaa gtttgcattg actgcttttt cattggcaat atccaaatga ctggccgctt
    12841 catctaaaaa taggattttc ggttcgcaat ataaggcccg cgccaatacg atacgttgtt
    12901 tttgtccgcc tgacagtgcg ctgcccatat cgccgatcaa ggtttcatag cccataggca
    12961 tggcggagat ttcttcgcga atcatagcaa ggcaggcgca atgttcgatt tttcctctat
    13021 ccggttctgt atcgaaaaaa cagatattct ctgcaattga gccagcaaat gcaaatatag
    13081 tggattaaat ttaaatcagg acaaggcggc gaagccgcag acagtacaga tagtacggca
    13141 aggcgaggca acgccgtacc ggtttaaatt taatccacta taaaacatcg tcctgcctga
    13201 ccatcgcact caaattgcgg ataaaacgtg gcggtaagct gtaaatgtcg tgcccattta
    13261 tcatgacttt gcctgattcg ggaggtagat tgccgcttaa aatatccaaa aatgtcgatt
    13321 taccgcagcc cgatcgtccg attaacgcaa gcgattcacc acgtccgatc ttgaagctga
    13381 cgttttgtaa aacatatggc tcgttatcgg cataacggaa agaaacattt tccactgaga
    13441 tttcgatgtc gtctgaaata tcaggaatgc ctacatccgg cgtagcttcc gattctgttt
    13501 cattcaatac ggtgtcagct aaccgttccg catgaagtga cagcatttta atttgcacaa
    13561 actgatcgac cagcgagcca atacgtcctt caaactgtcc cttataagcc aaaaaagcca
    13621 ttagtacgcc gacggtaaag acgccgtcca ataccatgcc cgcagctaag taaataatca
    13681 aaatatttgc caaaccgaaa agcagcttgt ttgccaagcc gaaccaaatt cccagtttct
    13741 gcaccgtcag tcccgtatta accgtatcca ccagcagccc catccatgca gaatatcgtt
    13801 gaggaccttt accaaattgc ttgactgctc ggataccgcg taccgtttcc ataaaatagc
    13861 tgctttgttt ggcagcatgg atgatgtttt cctccgtcgc ccgtcgcaac ggataatagg
    13921 ctacccaacg gactgcgata taaactacca gtgtcgctgc aaccaaacct gtcagtatcg
    13981 ggctgtacac tatcattagc acaaaagtaa aaacagccgt taggctgttt aaaaccaaca
    14041 cgaaaaacgt tgaagtcaga gttccctgaa tactatccac cgccccgaag cgcgatacca
    14101 catcgcccaa atgccgttta gcgaaataat ctgtaggcaa atctatcaaa cgtttgaaaa
    14161 cgtttgattt ccactgaaca tttaacgtcg ttgaaaaatg catgcccaac cagccctgca
    14221 tgacagacac cgtattttgt accagaagta gcaaggtaaa gcccaaagcc agcgtcgcta
    14281 acagattacg atcgcccgtt accactacat ggtcgatgac ccattgcata aaaaacggcg
    14341 acaccaaagc aaacacttcc attgctgctg ccaaagccaa aagctgaaac aatgtccgcc
    14401 ccagcccaga aatcccgcgc aacatgggta ggatgcggat ttcctgcttt tcttcccctg
    14461 cctcgaaacg cgtgtttggc cacagttcca aagcaattcc cgtaaacttg cgcgatattt
    14521 cctccgtctt gactttgcgt cgtcccgaag ccggatccat gacggcagcc ccgtccgaag
    14581 ataccgattc cagcaccaca aaatgattca aatcccaatg tagaatacag ggcaggcgca
    14641 aactgcccaa ttcgtctaaa tccagcctca acgcccgtcc cgtcagcccc atatcatcag
    14701 caaaacgaac aatatctgcc aaattctcac ccttaagtga cagacagtat tttgaacgca
    14761 gtgcgcgcaa atccgtatag aaaccataaa atccggccac agccgctaga catgccaagc
    14821 cacactccgc tacttctgtt tgcagtataa cgggcagctt ttttgtcaag cccaaagaca
    14881 ggttttgcag ataatccata gatttccttg ttgaatcaga aaagagagat gggaagacgg
    14941 gaatggtttg gaaaggccgt ctgaaaaggt ttcagacggc ctgcagcccc atattgactt
    15001 tcaatgcgac cggttttcct cgcgtttcaa atacagcaga tacgcaagaa aagacaaagc
    15061 ccctacaggt cctgcgacgg taaccgaaaa aaacctgcct ttgtaaaaca caccgtcgaa
    15121 gtttaaaaaa tagcaaagga tgtcggtcag gatgctgatg gcaagataaa tcagcacagc
    15181 ggctttccag tgtttcaggt aatgtgcgaa atccatttca aactcccgat caacagttgc
    15241 acgggtcgca atggcagtct tctccgcatt ggcagttgcg cctgtcgatc atttttcccc
    15301 attttgcacc ggcaaaaccg ccgatatgcc cacccaatgc agaaccgccc tcagctccta
    15361 cccatccgcc gcctgcgccg ccgatggcaa ttccggcagg atttccgcct gttaccaatg
    15421 cgccgcccgc cattcccgtg aatgctccgg caaacgaccc taaagcgccg ccgaccttgc
    15481 ttccgaccca ttctccggag actctgcccc tgtgccccca agtaccttct gtgccgccga
    15541 aaacagcggt catttcgttc atggtcaatt ctctcatttc aaaactcctt ttatcgtcaa
    15601 tcgaaaaatt gaaagggcag ataccctatc cttgctttta cagacagagt atctgtaaaa
    15661 gggggtagtt tcaggcagct tcagtgttta tgtttttatg tagtgtttta caggtcgtct
    15721 gaagaggttt cagacgactt tttatctttc agcctcaagc cttacaacct gcccgacata
    15781 ctataaatcg gctccaatac ccattcgtac agccgccgtt tctcgtgtag gatgtctgct
    15841 tccaacgtca tgccgatttg cagcggtttt tcctcaccgt atgcagtgat ggttggtttg
    15901 tcgggtttta ttttcacgag ataaacaggt tcgttgctct tcgccaaatc ggaggatacc
    15961 atgcccaatc ccgacaattc ctgtctgccc agtgccgttt ttgccactga tacgacactg
    16021 ccggaagcaa gcccgaattt ttgataggga tatgcctgat aacgcaggac aaccttgtct
    16081 ttcggcttga taaagcctgc tgcactgctg gggatatata gatgggcata taactcggta
    16141 cgttcgggaa caatgctcaa gaacagtttg gaaggatcaa cctgccgtcc gacttcgacg
    16201 ttcggtattg ctatataacc cgaccgtcct gcacggatga tttgttcaga gcgcatttca
    16261 aaatccaaaa cttcttgaga aatatcggca atggtgcgtt caagctggct ttgttctgtc
    16321 tcatgccgtt tggggaggct ggccaatgtc agattctgcg tgcggatttc ctgaagcagc
    16381 ccggcttctt ctcggcggta ggcatcaagt ttggctttct gctctaaaag ctctgcctcg
    16441 acattcatca tttcttgttt tgacactgca tcattggcgg ataggaaacg atatttccgc
    16501 aacatttctt ccgcaagtct aatgcgcctt ttctggccgt ctatctgttg cgaaatatgg
    16561 agtttctggt tttccaaacg ttcgacagtt gctttaaggc tgcgcgtttc attctcgtgt
    16621 atcagcttca gacgacccag ttcctgttct gccaacgttt tcttcaaaac tgcctccgtt
    16681 ttcaactgct gctgcacgct acctcctgcg ccgaaacgtg aggtcgaaag cgcaaatagc
    16741 ttgtcgccag ccttaacctt ttctccatct tccacgaatt tcgctgtaat tgtccccgta
    16801 tccggtgcat acaccctgat tacgcccgat gcaggtagaa tttgtccctc cattgttgtc
    16861 tttcgcgtat agttaccgaa tatcaaaaac aggataatca gtaacgcaga tatcgatgca
    16921 aatgtcgtcc acagggaaaa tgacaacggg cgtgtcagaa tcactttacc cgtcaggctg
    16981 gtttggcggg caatggcgac ttcagggcgg aagaaaggtt gcttgggtct attcataaaa
    17041 ttgaagttaa gaaagtttca gacgacccct agagatcgtc tgaacaatga gaaatatcag
    17101 cagtaatctg taccgtcagt gtagccgttt cctgatttat ctgcttttgt tgcgggagca
    17161 gttaatccat gttcaatctc aaagattggt cttccgttat aaggaggtgc attaacggca
    17221 tcatttaccc aatcacgagt cagattgtat acaccatttg caccatatag caccgaaaaa
    17281 atttttcggc aggaaatgaa ctgtcgctgc agcagcaggt attgcaacaa aatcccccca
    17341 tgtgggacct cctttggttg tggcagcatt agctacattt ccagctatat tgtctgttac
    17401 aggacctccc cctgaaacca gcttcaattc atgaagttga agttcaatca tttttatact
    17461 ccttttcttg gttggtattc ctaaaaattc ggctaacaaa aacatatggc agatatattg
    17521 aaaaaaaatt caaagtaccc tgaataaaat tcaaattcca actatatttg ttaatgtagt
    17581 cgagaagaaa catatctgat aaagaatata gcaattgata acaagctatt actaatatta
    17641 cgaaaaatat aaactgcttc cagtttttca tagaatccct cacaaaattt ccagaaaatc
    17701 taactctatc aactgataga tcaacttcct aacttcttca tgttttgcct gattgaagtt
    17761 aaccagtaga tttttcaaca ataacggttc attcttaccg atgtgttcta acactttttt
    17821 ccccaactca tctacgctta tcttcatccc attcccaatc aaatatccct tttccaacgt
    17881 atccaaatta ttggcattta gtctcaacct gacgtcgtct gaaagcggag tagcgttggg
    17941 attcgcgaac tgttcgagat gaaaagcggt atcggtacgt tctttgccga gaaagtcttc
    18001 actgaaagct tcgtaattga cggggtcggc aatcatggcg gcaatttgtg cggcagtatc
    18061 gttgatacgc gtcctatctt gctcccagtc tgagaaactg tggcgcagac tttctatggt
    18121 gggaaatttc ttcattagcc actcgaggta attatagccg tttggtggga aggtaccgac
    18181 agcgaagtgg aaggtttcac agccgagcgg gataggtctg tgccaccaac cgcgtgggat
    18241 gtagaggaca tcacctgctt caaggataat atccatatcg atatgttcag gaatggaaat
    18301 atcagtatct ttagtctgtt gcatatacaa tggcataggg aaatcagggg cagtaagttg
    18361 ccaacgtttc ttgccgaaaa gctggacggc atacatatct cgggcgtccc aatggttttt
    18421 ataagattcg tcgctgccaa aagcaagata tccactaaca atagtatgtg cgccggcaaa
    18481 gcgggcgact tgacgggcga tatggtctga aaacggctcg ttgttaatat ggttatagac
    18541 taacgacgca ccgttcttca tatgttcgta gataacggat ttaataaaac ggtagcgagt
    18601 tttgcccaaa tcgtcgaaac tttcgacgta ttcttcttta ggaacgattg cgcctttttt
    18661 acgcagatga aacagcggtg cggttgggtc tgctcgttgg tatatctcgt tgatatattt
    18721 acaagatgcg gcttcgagat tccgaaccgc tcctttaaag agcttgggct tttgatacag
    18781 ataagtctgt cggaacgttt taggactaat gccgaagtcg agatggatgc ccattacttc
    18841 c
//
LOCUS       DAWTHD010000028        18714 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWTHD010000028 DAWTHD010000000
VERSION     DAWTHD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18714
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.028"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            332..1384
                     /locus_tag="WM704_000785"
     CDS             332..1384
                     /locus_tag="WM704_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFA9340247.1"
                     /translation="MRQYLPYSVRWCFFINPGAIILHRQIFLELMLPAAYGGFLTTAL
                     LDRTGFSGNLKPAATLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAWLIWLDR
                     NTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSVLLGTETLK
                     ECRLKDPVFIPNVIYKNIAITLLLHAAAELWLPAQTAGFTALAVGFILLAKLRELHHH
                     ELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMTGGVMMVWLTA
                     GLWHSGFTKLDYPKLCRIAVSILFASAVSRAVLMNVNPIFFITVPEILTAAVFMLYLL
                     TFVPIFRANAFTDDPE"
     gene            complement(1431..3620)
                     /locus_tag="WM704_000786"
     CDS             complement(1431..3620)
                     /locus_tag="WM704_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFA9340248.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPIGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTGQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            3760..4545
                     /locus_tag="WM704_000787"
     CDS             3760..4545
                     /locus_tag="WM704_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFA9340249.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(4644..6578)
                     /locus_tag="WM704_000788"
     CDS             complement(4644..6578)
                     /locus_tag="WM704_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFA9340250.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6644..7822)
                     /locus_tag="WM704_000789"
     CDS             complement(6644..7822)
                     /locus_tag="WM704_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFA9340251.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8048..8437
                     /locus_tag="WM704_000790"
     CDS             8048..8437
                     /locus_tag="WM704_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFA9340252.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFEKYDSTKLKQNKYFKITLIKASPDHFTLQAAPDTATNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(8531..12016)
                     /gene="smc"
                     /locus_tag="WM704_000791"
     CDS             complement(8531..12016)
                     /gene="smc"
                     /locus_tag="WM704_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFA9340253.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIAAELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            12346..13526
                     /locus_tag="WM704_000792"
                     /pseudo
     CDS             12346..13526
                     /locus_tag="WM704_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            13735..14457
                     /gene="lpxH"
                     /locus_tag="WM704_000793"
     CDS             13735..14457
                     /gene="lpxH"
                     /locus_tag="WM704_000793"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFA9340254.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            14726..16312
                     /locus_tag="WM704_000794"
     CDS             14726..16312
                     /locus_tag="WM704_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFA9340255.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16478..16681)
                     /locus_tag="WM704_000795"
     CDS             complement(16478..16681)
                     /locus_tag="WM704_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFA9340256.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            16797..17294
                     /locus_tag="WM704_000796"
     CDS             16797..17294
                     /locus_tag="WM704_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFA9340257.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            17376..18566
                     /locus_tag="WM704_000797"
     CDS             17376..18566
                     /locus_tag="WM704_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HFA9340258.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKV"
BASE COUNT         3995 a         5381 c         4951 g         4387 t
ORIGIN      
        1 gggcatttca gtatctgaca aaccttgcgg ttcgtttccc tctccctaac cctctcccac
       61 aggagagggg atcaggttgc tgaaaatcag agggcgaagg attggggatc aaatggaagg
      121 taaaacctga aaatgttcgg gtttgacagc ccgattccct ctccccgtgg gagagggcta
      181 gggagagggc aagcaagccg caggcttgcc tctttggcga aaaatacaac cctgtccgcc
      241 caataaatcc atacccgaaa gcatcttcat acagaatcaa gccaagcgat gaataaattt
      301 tttacccacc ctatgcgccc gtttttcgtc ggtgcggcag tacttgccat actcggtgcg
      361 ttggtgtttt ttcatcaacc ccggcgctat catcctgcac cgccaaattt tcttggaact
      421 tatgctgccg gctgcatacg gcggtttttt gactaccgct ttgttggacc ggacgggttt
      481 ttcaggcaac ctgaaacctg ccgctacttt gatggcggtg ttgttgcttg ttgcggctgt
      541 tttattgccg tttttaccgc aacttgccgc atttttcgtc gccgcctatt ggctggtgtt
      601 gctgctgttc tgcgcctggc tgatttggct cgaccgcaac accgacaact tcgctctgtt
      661 gatgttactt gccgcattta ccgtttttca gacggcctat gccgtcagcg gcgatttgaa
      721 cttactgcgc gcgcaagtgc atttgaatat ggcggcggtc atgttcgtat ccgtccgcgt
      781 cagcgtcctt ttgggcacgg aaaccctgaa agaatgccgt ctgaaagacc ccgtattcat
      841 ccccaacgtt atctataaaa acatcgccat caccctgctg ctgcacgccg ccgccgaact
      901 ttggctgccc gcgcaaaccg ccggttttac tgcgcttgcc gtcggcttca tcctgctcgc
      961 caagctgcgc gaactgcacc atcacgaact cttacgcaaa cactacgtcc gcacttatta
     1021 cctgctccag ctctttgccg ccgcaggtta tctgtggaca ggcgcggcga aactgcaaaa
     1081 cctgcccgcc tccgcgcccc tgcacctgat taccctcggc ggcatgacgg gtggcgtgat
     1141 gatggtgtgg ctgactgccg gactgtggca cagcggcttt accaaactcg actacccgaa
     1201 actctgccgc atcgccgtct ccatcctttt cgcctccgcc gtttcgcgcg ctgttttaat
     1261 gaacgtgaat ccgatattct tcatcaccgt tcccgagatt ctgaccgccg ccgtgttcat
     1321 gctttacctg ctgacgttcg taccgatttt tcgagcgaac gcgtttacag acgatccgga
     1381 ataaaaatgc cgtctgaaac ggttttcaga cggcatcgga ttgccaatga tcaagcctcc
     1441 tgcggatcga catccaccga ccatcggatt ttgccgtcgc ggttctgctg caacacctgc
     1501 acccacaaac tcacggcgcg gtgcaaatct tgtcgggatg ttgattcgag gaaaacttgc
     1561 gcacgttcgc gttcggcaag gcgcaccatc agcatcgggg cggcaccgaa ccgggagacg
     1621 ctttcgggca agagcggggc gagggtttct ttggcggcgt tgagaaattc catcgcatcg
     1681 gcaacacgcg gcgcatcggc acggacggcc gtctgaaaac cgaaaggcgg catggcgaac
     1741 atttgccgtt cgttcaattc gttttcggca aacacggcgt agtcctgcgc tttgacggcg
     1801 gcgaagacag ggtgttcggg cagttgggtc tgtatcagca ccttgccggg tttgtcggcg
     1861 cgccccgccc tgccggacac ttgcatcagc tcggcaaaca gcctttccgg cgcacggaaa
     1921 tccgcgctgt acaggctgcc gtcggcgttc aacacgataa cgaggttgag ccgcgcgaaa
     1981 tcatggcctt tggcaagcat ctgcgtgccg accagaatgt cgatttcgtc gttggcgatg
     2041 cggcggtaca aatctgccca gtcgtttttg tgcgccgtac tgtccctatc gacgcggacg
     2101 acggctgcct tgggcaggaa ggcgcgcagg gtttcttcga cgcgctgcgt accgtgtccg
     2161 acggcggtca ggtcttggtt gccgcagtcg ggacatttga acgggatggg ttcgcggtgg
     2221 tcgcagtggt ggcagcgcag ttggcgggcg cgttggtgca gcaccatttt ggcggagcag
     2281 ttcgggcagc cgaaggtatg gccgcagtcg ccgcaaaaca gcgcaggcgc gaagccgcgc
     2341 cggttaaggt acaccagcga cataccgccc gcttcaaagt tctgtttcaa aagctgcaag
     2401 gcttgcggtg agaagccgtt atcgagtttc agacgaccta tgttgaggat gtccacttgt
     2461 ggcagttgcg cggaagcatg ggcgcgttcg gtcagttgca gcaggcggta cgcgccgctt
     2521 tgcgccttgt gccaactctc caagctgggc gtggcactgc ccaacacgac gggacagccg
     2581 ccctgcttcg cccgccacac cgccaaatcg cgggcgtggt agcgcaattc gttgtcttgt
     2641 ttgaacgagc cgtcgtgttc ctcatcgacc acaatcagcc cgacatcagg caaaggcgta
     2701 aacaccgcca gccgcgtgcc gatcaccagc ttcgcctgcc ccaacatcgc gcgcagataa
     2761 tcctgcgtgc gcctgcctgc cgccatccgg ctgtgcaaca cggcggtcgg cacgtcggca
     2821 aaacggtttt ccacccgctt taaaagctgc ggcgtgaggt tgatttcggg caacagaaac
     2881 aacacctgtc gcccctgcgc caacactttc gccatcgcat cgaaatacac ctcggtcttg
     2941 ccgctgccgg taataccgta cagcagaaac gaccggaagc gtcccaatgc cgtctgaatt
     3001 tcatcggaag ccttctgctg gccggtattt aatacaaatt cagagtgcga agcctgcccg
     3061 cggtacggcc ttaaaacagg tttcgccgct tccgtcgttt caatccagcc ctgctccgcc
     3121 caatcctcaa tcaatttcgc cgcctgcgcg tttgcctgtt tcaacgccgc catcgtcatt
     3181 tcgcccgaca gcagcgcgtc ccacaaagcc gcttttttgt tgaaccgagc cggcggcggc
     3241 gtttgcgccc tgcccgcttc gttcaaagca taaaacaacg gcggctgcgg catttccacc
     3301 gcgcgcgttt ccttcaaacc ctgcggcaat gcggcaaata cagcctgccc aatcggatag
     3361 tggtaataac gcgacgtaaa cgccaacaaa tcacgccagc tttgagacag cggcttttct
     3421 tccacaaaga ccgtctgaac gctcaaaatc cgcgccgcat ccatatcggg cgcaatatcc
     3481 gtttcccaca ccatcccgac aacagtttta ttgcgaaaag gcacaagcac ccgcgttccc
     3541 ggaggaagcg gctcggaatg cgaataagtc aaaaggccgt ctgaaagcgg cacgtttaca
     3601 gcgatgcgat ggtagatcat acaggcaggc aaatcatttt tttgcgtatt ttaacagccg
     3661 gaagctattt tcagacgata aattgagcct cggcgcaatg caggcgcaac agaggtttga
     3721 tacaatgcca cttttccaac acgctcaatc atctttgata tgaaaaccaa gttaatcaaa
     3781 atcttgaccc cctttaccgt cctgccgctg ctggcttgcg ggcaaacgcc cgtttccaat
     3841 gccaacgccg aatccgccgt caaagccgaa tccgccggca aatccgttgc cgcttctttg
     3901 aaagcgcgtt tggaaaaaac ctattccgcc caagatttga aagtgttgag cgtcagcgaa
     3961 acaccggtca aaggcattta cgaagtcgtc gtcagcggca ggcagattat ctacaccgat
     4021 gccgaaggcg gctatatgtt cgtcggcgaa ctcatcaaca tcgacacgcg caaaaacctg
     4081 accgaagaac gcgccgccga tttgaacaaa atcgacttcg cctccctgcc tttggacaaa
     4141 gccatcaaag aagtacgcgg caacggcaag ctgaaagtcg ccgtcttctc cgaccccgat
     4201 tgtccgttct gcaaacgctt ggaacatgag tttgaaaaaa tgaccgacgt gacggtttac
     4261 agctttatga tgcccattgc cggcctgcac ccagatgccg cgcgcaaggc gcaaatctta
     4321 tggtgtcagc ccgaccgtgc caaagcgtgg acggattgga tgcgtaaagg caaattcccg
     4381 gtcggcggca gcatctgcga caatcccgtc gcggaaacca cttccttggg cgaacagttc
     4441 ggcttcaacg gcacgccgac cctcgtcttc cccaacgggc gcacccaaag cggttacagc
     4501 ccgatgcccc aactggagga aatcatccgc aaaaaccagc agtaaacccg caatgcaaaa
     4561 ggcttggaac catgttccaa gcctttttca aatgccgtct gaagcctgtc gcggcagctt
     4621 cagacggcat ctttgttcaa acctcaatcc tgcgccaatg cgtctatcgg attgagtttg
     4681 gctgccttat tggcgggcat aaagccgaac gcgatgccga ttccggtcga acaggcgacc
     4741 gccccgataa cggatgccgc cgaaatgtcc atcgggaaat cggttacaaa atgattgaac
     4801 acgaggctga cggcggcgga caaacctacg ccgaccaagc ctccgatgat gcagattaac
     4861 accgcctcaa tcaaaaactg ctgcaaaata ttgccgcgcc gcgcgccgat tgccatccgt
     4921 atgccgattt ctttggtgcg ctcggtaacg gacaccagca tgatgttcat cacgccgatg
     4981 ccgccgacta ccaatgaaat cagggcgatg gaggaaatca gcagcttcat cgtaccggtg
     5041 gtgctttcga ccatctgcct gatgctgtcg ctgttgttca taaagaagtc ttccgtgccg
     5101 tgccgtgctt tgagcagctc ggccagccct ttttcggcaa cccgggtatt ggcattgtct
     5161 ttgattttga cggtgatgga gttggtgtgg ctctcgcctg tgatttggtg catcaccgtc
     5221 gtatagggcg accaaagcat cagcacgtcg gaattgccga aagcgttttc gtcttttttc
     5281 atcacgccga tgacggtcaa ggggcgtttc ctgaacaaaa tggttttacc caacggatcc
     5341 gagtccgcaa agagtttgtc tttgacattt tggtcgatga cgacgacttg cgcgtcttct
     5401 ttcacatcgt tctcatcaaa cagccgcccc gtttccagct tcagcccgcg cacgtcgaaa
     5461 tattgttcgc ccacgccgta caaagaagcg gtcaggtcgg tattgcggta ggtcagcgtg
     5521 ccgccgctcg aagtcatggg cgtggcggag gcaacgtagc tttgtttggc gatgattttt
     5581 gcgtcgtcta tggtcagggt tttgattttg ccgctgcgcc tgtcgccgaa gccgcgcccg
     5641 gggaagatgc tgatggtgtt cgtccccatc gaactgatgt cttcgaggat ttttttctgc
     5701 gaaccgttgc ccaatgcgac gacggagaca accgaagcga taccgatgat gatgccgagc
     5761 atggtcagaa gcgaacgcat tttgtgcgcc aatactgctt gcaccgacat tctgaaggct
     5821 tcgacaaact ggtcgtaata aaacgaccac gaggcttttt cctgaatcct cccgacattg
     5881 cttgcgggga tttcgggatt tttcgaggtg tcggaaatga tttcgccgtc ccggatttcg
     5941 atgacgcggt tggcgttggc ggcaatgccg gggtcgtgcg tgaccataat gacggtatgc
     6001 ccggcttcgt gcagcctgcg gatgatttcc atcacatttt tgccgctggc ggtatcgagt
     6061 gcgccggtcg gctcgtcggc gaagatgatt tctccgccgt tcatcagggc gcgggcgatg
     6121 gagacgcgct gctgctgtcc gcccgagagt tcgccgggct tgttgccctc tttacttgcc
     6181 aaacccaaat cctgcaagag tttgtccgcc cgcgcggaac gctctttgcc gcccataccc
     6241 atatacacgg ctggcagcgc gacgttgtcc cttgcggtca gcgagcttaa gaggttgtag
     6301 cgttggaaga tgaaaccgaa gcgttcgcgg cgcaatgccg ccagttcgtc aggctgcatt
     6361 ttggcagttt cgatgccgtc gattcggtac gaaccggaac cggcggtatc caaacaaccg
     6421 agtatgttca tcagcgtgga cttgcccgaa ccggactgcc cgatgatggc gacaaaatca
     6481 cccttctcta tcgacaggct gatgtctttc aaaatatgga cgcggttctc gccgctgccg
     6541 aagcagcggt tgatgttttt acattcgatc aagctcataa tattccgtaa atcaataaca
     6601 aatgccgtct gaaaccgttt ccgtgttcag acggcatatt cgtttatcgg cgcggcgggc
     6661 cgcctagggc gcgttcgccg ctttcctgct gctcggcggc ggttatttcg gagatgacca
     6721 ctttgtcccc ctctttcaac ccgcttttca cttcggtatt catactgtct ctcataccgg
     6781 tccggatttc gcgttccgcc gccttgccgt ccgcacccaa cacgcgtacg aacgccttgc
     6841 cgccgcgatt tttcacggtc agcgacggaa taagcaacac atttttcaca ccgtcgattt
     6901 caaccgtatt ctgcgtcgtc atccccgtgg cgagtttgcc gtccggattc ggcacaaacg
     6961 aacgggcata ataatagacc gcattggaag ccgtatccgt actgctgttg tagccgcccg
     7021 acgacatcgt ggtcagcccg gggtcgacgc tgtcgagctt cgccttaatc ggcgtatccg
     7081 gttcggacaa aatcgtaaac gaaatatcct gccccgcctt caccttggta atatcgccct
     7141 cggcaatctg cattttgttc aacatcatat ccagattcgc caattggaca atcgtcggcg
     7201 tagactgcgc cgcgttcaca gtctgcccct cttccacggg aatcgccacc accgtgccgt
     7261 ccatcgtcgc ggtaatgcgc gtgtagccca aatccgactc ggcggtattg atggaaattt
     7321 tgctctgtct gattaaagcc ttcaactcgg caacattggc tttggcggcg gcaagcgcat
     7381 cctgcgcgct ttccaaatct tctttagagg tcgcatcatc cttccacaac gccgcctgac
     7441 gcttatattt cttctccgcg ctgcccaatg caatctgtgc cgacaccagc ttcgcctgat
     7501 acgtttccaa tttggatttt tccatatcga gcgtgttggt ctgcgtggtc gaattgattt
     7561 ccgcaatcaa atcgcccttt ttgacctgtt gcccgagttt gacataaagc tttttaatct
     7621 gccccgaagc ctgcgcgccg accgatacca ggttggacgg cgaaatctcg cccgtcgcgg
     7681 aaaccgtccg gctgatgccg ccgcgcctga ccgtttccgt aatataagaa gcctgcggct
     7741 cgggcttcag ataatgccat ccgccccaaa ccgctgccgc cgcgactgcc gcaacagccg
     7801 accatttcat cattttcgcc atattttcaa ttctctgtat gcgtcaatcc cataaaccgg
     7861 aagcggattc taccgcaagt ccctgcccca tccaaggcgg caaaccgccg acaataaaaa
     7921 taaccatttg ttttaaatgg cttatacaat ctgctttccc gaaaatttca atccgtttac
     7981 atattccgcc atttccgata cggcgttata atgccgccca caaacaacca aatggaaaca
     8041 cattgctatg aaaaacgttc aaaaaggctt tacgctgctc gagctgatga ttgccgtcgc
     8101 catcctcggc atcctgacgc tcatcgccta cccttcctat aaaacctaca tccggcgcgc
     8161 ccgcctgtcg gaggtcaaat ccaccctgct ggtgaacgcg caaaacttgg agcgttacta
     8221 ccgccaaaaa gggacgtttg aaaaatacga ctcaaccaaa ctgaaacaaa acaaatattt
     8281 caagattacc ttaatcaaag ccagccccga ccacttcacc cttcaggccg ctcccgatac
     8341 ggcgaccaac gaaggcgaaa cctgcaccgt cacgctcaac gacggcggta ccattgccgc
     8401 ttccggtaca aaccaatcct gccccggctt cgactgattg gctgttgcag tcggcattgt
     8461 gcctaaacca tctataaaaa tgccgtctga acgctgttca gacggcattt ttgacatccc
     8521 gatacgcctt tcaaacggct tccgccattt ccaacgcctg cttgatgtct acggcgacga
     8581 cgcgcgacac gcctttttcc tgcatggtta cgccgaccag ctgctccgcc atttccatgg
     8641 tcaggcggtt gtgggagatg tagaggaact gggtttgcac cgacatttct ttgaccagct
     8701 tgcagaaacg cgaggtgttg gcatcgtcca gcggggcatc gacttcgtcc agaaggcaga
     8761 agggggcggg gttgaggctg aacagggcga acacgaggct catggcggtg agggcttttt
     8821 cgccgccgga gaggaggtgg atggtgctgt ttttcttgcc gggcggacgc gccatgatgg
     8881 acacgccggc ggtcaggagg tcgtcgccta tcattttgag ggtggcttcg ccgccgccga
     8941 acagggtcgg gaagaaggtt tggactttgc cgttgacggc gtcgaaagtt tctttgaaac
     9001 gcgctttggt tttgtcgtcg atttgggcga tggcttcttc caaaagcgcg atggcggcct
     9061 gcacgtcttc gctctggctg cggtagtagc cgtcgcgttc gcgcgcttct tcgagttctt
     9121 gcagggcggc gaggttgacg gcgccgaggg cttcgatttg ccgggtaagg ctgccgatgc
     9181 tgctgttcaa tactttcgcc gattctttcg ccaacgcttc gagcacgtcc agatcggcag
     9241 cgcgttcggt caggttttga tggtagcgtt tggcgttgat cagggcctcc tgctgctgca
     9301 acagggcggt ttgggtggcg gcctgaagct gcggcagctt ggtttgcagg gtttgtacgc
     9361 gggcgtattg ctccctgccc tgttcctgaa tctgcgcgag tttttcctgc acggcgatgt
     9421 attcttcgtc cagcgtgtgt acggtttcgg tcaactcgtc gagcttgatg tgctgctcgt
     9481 cgttttggaa ctcggtttca taggcgaggg caagctcttg ccggcgttcc tgccagtcca
     9541 gggtttgctg ttcgagccgg gcgatttgct gccggtagtt ttgtttttgc tggttgagtt
     9601 tgtggacggc gacttcggca agcccgtatt ggcggttggc ttccaacagg gcaagctgcg
     9661 cctgtttcag acggccttgc tgctcttggc ggctgtgcgc ggtggtttgc tgctggtgtt
     9721 cgagttcggc ggcggcttcc tgcaaagtag cgatgtcgtc tgaaagcccg tcggacgtgt
     9781 gttgcaacac ggtctgctct tccgccaact gtgccagttc gcgctcgatg tgttcgcggc
     9841 ggatttgccc ttggttggtg cgcgccagaa gttcggcggt gcgttgctgc gcttgactgt
     9901 attggcgcgt gtgttgctgt tgctgctgca tcagattttt gtgctgcact tcggacgagc
     9961 gcacggcagc ctccgcctgt ttgaacgcgg cttcggcggc ggaaagtccg ggggcgaggt
    10021 tttccagttc ggccgcgatg ccgtcgaggc gtgctttttg ggcaatcagg ctttcctgcg
    10081 cgggcttggc atagagcagg acgctgactt tatcgacctg atggccttcg ggtgtgagcc
    10141 agatttgatg cgtgcccaaa tcgctttgat gcgcgagggc gtagctcaaa tcgggcgcgc
    10201 acaatacgcc gtcgagccag tggtgcaatg ccgtctgaaa cggcggctgc gcttggattt
    10261 ggttcagcaa tgcctgtacg ggcagggatt ttttgatgcc gccggagagg ccgtctgaaa
    10321 gccatgccgc ctgcccctgc ggcaaaggtg cgggcggtac gaaactattc ggcacgctac
    10381 gggcgtgcag gcgttcggca aggatgacgg acagggcgtg ctgccactcg gcgggcgcgg
    10441 tgatgtgttg ccacagttgc ggcgcggcgg cgtggtcggt tgcctgccaa aagtcggcgg
    10501 cttcctgctg ttgcgacagg atttgcgaca acgcctgctg ctgcgcctgc aaggtaatgt
    10561 gttgctgctt caggctttgg aagcggtttg aggccgtctg aaacgcctcg cgggcggcgt
    10621 gcaaggcttc ttcggcggca atgatttgtt cttcgtaatg ctcttgccgg ctttgcaaca
    10681 aggcggcggc ttcctgagcg gcggcggttt cggcctcgtc gggcaggttc agggcttggt
    10741 tttcctgctt cagacggcct ttgcgctctt cgtgcttggc aacggtttgt tcggcatggg
    10801 caagctgctg ctgcttcaac gccagttcgc ggcggatgcg gtttgcctcg tcctgctggg
    10861 tttggaaggc agcgttgagc gtggcttgga cttcttccaa ttcgggcaga cgctcttcgt
    10921 gttcggcaac ctgcatcgcc cattccgcca attcggtttg cttttcttcg acctgcaact
    10981 cgttttcttc aagctgcacg cggatttgct gctgctcctg ccggatgcgt tgcaactgcg
    11041 cctgcgccgc ctgcttgtcg cgttcgatgc gttggtgcag gttttgccgg tggcggattt
    11101 gttcttccaa acgggcaatc tgctcgcgca acacgccgcg tttgttgctc aattcgtgta
    11161 ctgcctgctg ctgcgactgt tcggcagtct gcaaggcgtg tacttcgtta tttaacgcct
    11221 gaacctgtgc ggcggtttca tcctgctgcg cctgcaaaga ttgatgctgc gcggtcgcct
    11281 tgtcggcggc ggcaagcgat tgctgccatt gggcgtaatc gagcaaatcc tgttgttgat
    11341 tcaactgcgc ggtcagggat ttgtagcgtt cggcggtttc cgcctgtttt tccagctttt
    11401 ccacctgacg cgccaactcg ttctgcaaat cgcccaaacg ctgcaaatgc tcgcgcgtgt
    11461 ctttcagacg gccttccgtc tccttgcggc gttccttata tttggacacg cccgccgcct
    11521 cctcgatata ggcgcgcaac tcctccggcc gcgcttcgat gatgcgcgaa atcatcccct
    11581 gctcaatgac ggcataaccg cgcgcgccca cgcccgtacc caagaacaaa tcggtaatat
    11641 cgcggcggcg cacggtctga ttgttgatga aataagtcga ttccccctga cgcgtcagct
    11701 gccgcttgat gctcacctcg gcatactgcc cccacgcgcc ctgcaaactg tggtcgctgt
    11761 tgtcaaatac cagctccacc gaagccctcg gcgcaggacg gcgcgtcgcc gcaccgttaa
    11821 aaatcacgtc ctgcatactc tcgccgcgaa gctgcttcgc cgaagcctcg cccaacaccc
    11881 agcgcaccgc gtcaatcaca ttcgacttgc cgcagccgtt cggcccgata accgcgacaa
    11941 gctgccccgg aacatgaatc gtggtcgggt cggtaaaaga tttgaagccg gagagtttga
    12001 tgtgggtcag gcgcataatg gtcggaaaaa ataaaaaaga agcgtatttt aacggaaatc
    12061 ccgccgcacc accccatatc ttgccggcaa aaccttaccg catcccgccc cggtgcggca
    12121 ggattttgca catcccggcc atccgtatta aaatctttgc gtttcccaca ctttcctgac
    12181 aaatatacgg aggaggattc catgaaaaca aaaacagtca agcaagcaag caagcaagca
    12241 agcaagcaag caagcaaaat agtctaaccc gcctttcttt aatcgctcta cttaatttcc
    12301 acttcttttt atcctgttcg atcttttgcg gctgcacctc aagaaataaa ctattcgtta
    12361 tcaggcggta aagtttacgc agataagttc agtatcgcca ttggggttga ggcaaaagct
    12421 cagggggata ataccaaact tagcaacctc aatcggcaca tctgccgaag caaatgcacc
    12481 cggggcattg gctttagggg gaaattctga agcatcgaaa aaatttagta tagcagaagg
    12541 ctatctggca tctagtgatg gctatggtgc aatagcgatt ggttctgccg ccaagataaa
    12601 gcaattggaa aaaggcacaa taaatcatat tgtgggaaac gataataaag gtctttatgt
    12661 cgatgcagac ggcaatgtaa caaagataac cgtaaggact gagtcggaaa aagatattct
    12721 ctcaagatac ggtcaaactt acggtgcggt ggcactaggg tttagatctt cttcacataa
    12781 tctttttgcc agttcatttg gagcgttttc tacagccaca gctattgaaa gcttggcagt
    12841 cggcgacagc agccaatcaa cgggctaccg cagtgctact tttggcagtc acagcagggc
    12901 tttggcagaa gaaagtttgg cattaggtta tgaaactcgg gcaaatgctt atggttccgt
    12961 tgctttaggt gcagaatctg tggcgaatga agaaaatacc gtatcagtgg gttccgatac
    13021 attgaaacgg aaaatcgtca atgtcgccga tggcacggaa gatttatgat gcagtaaatg
    13081 tccgccagtt gaaccgctta agcaaacgta caaaccgcgt cggcgcaagt gctgcggcgt
    13141 tggcttcgtt aaaacctgca caattaggca aaaacgacaa attcgccttt tctttgggct
    13201 ttggaagcta taaaaatgcc caagcggtgg caatgggggc ggtgtttaag cccgctgaaa
    13261 atgtgttgct taatgtggcg ggcagttttg ccggaccgga ccgggctttc ggcgcggggg
    13321 tttcttggaa attcggcggc aaaccgacac ctgcggttgc cgcacaaaac gcggcgcatc
    13381 ctgcaaaagt tttgcaattg cggcaggaag tggcggcgtt gcgcgcccgg caggcagaaa
    13441 ccgaccgcaa actgcacaaa caggccgaaa tggaaaacga gctgcaacag ttgcgccgcg
    13501 cgttgtccga attgaaaaaa cattgaagca ataaacgtaa ggcgggacgg cggctctgcc
    13561 tttttccgat gccgcacccg cgcgatgccg tctgaagcct tcagacggca tttttaacgc
    13621 gcccgaaccc cggttccgcc aatgcccgat gcttggcgcg gcatttgccg tattgccccg
    13681 ccgccgggcg cgttataatc cgccacgaca tccgcccgat ttggaaacct gcctatgcga
    13741 ccctcctatt tcatttccga cctgcatttg agcgaaaagc accccgaact gaccgaactg
    13801 ctgctgcgtt ttttacgttc tgccgccgcc gggcaggcgc gggcggttta tattttgggc
    13861 gatttgtttg atttttgggt gggcgatgac gaagtttccg agttgaatac ttcggttgcg
    13921 cgggaaatca ggaaattgtc cgacaaaggc gttgccgtgt tctttgtcag gggcaaccgc
    13981 gatttcctaa tcggtcggga tttttgccgg caggcgggta tgacgctgct gccggattac
    14041 tcggttttgg acttgttcgg cagcaacacc ctgatttgcc acggcgacac attgtgcacc
    14101 gacgacaagg catacctgcg tttccgccgc atcgtgcatt gcaggcggct gcaaaaactg
    14161 ttcctgatgc tgcccctgaa gtggcgcacg cgccttgccg ccaaaatcag gcgtgtcagc
    14221 aaaatggaaa aacaggtcaa gcccgccgat attatggatg tcaatgccgc ctttaccgcg
    14281 cggcaggttc gcgcctttaa cgcggaaagg ctgatacacg gacacaccca ccgcgagcat
    14341 atccatcacg aaaacggctt tacccgcatc gttttgggcg actggcataa cgactatgct
    14401 tcaatcctcc gcgtggacgg ggacggcgcg gtattcgtgc cgccggaaga atgctgaaaa
    14461 tgccgtctga agcctttcgg gcggcttttt ctacacgccc ctttcccaca ctccctccgc
    14521 cgcatcatca ccatccttaa cccgcattaa tagccattat cgataaaatg cttgaccgat
    14581 tacataagat tacgtaaagt gtgcaaacgc ataataattg gtcttaccaa tttatctttt
    14641 aaccaaaata tcgggtgtgc gggatttgaa cgtctttcca aacctcgacg cgccttatga
    14701 tgcaaatgcc taaaaaggat tcgttatggc acttttcctc agcatattcc ccatcgtcct
    14761 gctgatttgg ctgatggtga aaaaaaacag tatgccttcc tacgtcgcac tgccgattac
    14821 cgcagtgctg atttacgcca tcaaactttt ctacttcggc gatgcgggaa tgctgctcaa
    14881 cgccaccgcc gcttccggcc tcgtcaaaac gctcacgccg attaccgtga ttttcggcgc
    14941 gattatgttc aaccgtatga tggaaaccac gggctgcatc gatgtcatcc gcaaatggct
    15001 ggcgaccatc agcccgaacc ccgtagcgca actgatgatt atcggctggg cttttgcctt
    15061 tatgattgaa ggcgcatccg gtttcggcac gcctgccgcg attgccgcgc cgattctgat
    15121 gagcttgggc ttcaatccgt tgaaagtggc gattttcact ttggtgatga attccgtgcc
    15181 ggtatcgttc ggcgcggtcg gtacgccgac ctggttcggt ttcgcaccgc tgaacctgag
    15241 tgccgaagac atcctcgcca tcggcaggca gaccggcgtg atgcacttct tcgcaggttt
    15301 cgtcatccct gtcatcggtt tgggcttcat cgtgccttgg tctgaaatcc gcaaaaactt
    15361 gggcttcgtc gccattgccg tcttctcctg caccattcct tatgccgcat tggcgatggt
    15421 caacgaagaa ttcccgtcgc tcgtcgccgg cgcaatcggc ctgatggtgt ccgtattcgc
    15481 cgccaaccaa ggctggggct tgagcaaaga ccacgccaaa gacccgaatg ccgaaaaagt
    15541 gccgttcgcc caagtcgcca aagcactcgc ccctttgggt atgctgatcg gcatgctggt
    15601 ggttacccgc atcaaacagc tcggaatcaa aggcattttg accagcaaag aagaatggtt
    15661 cagcttccaa ctgccgtttg atttgtccaa aattaccgtc agcgactccc tgacgattac
    15721 cttcggcaat attttcggac aagatgtcag cgcgtcttac caaacgctgt acgtcccggc
    15781 ttggattccg ttcgtgctga ccgtttggat ttgcatcctg ctgtataaaa ccaaattcaa
    15841 agatgcctgg acgatttatg ccgtaacctt caatcaaacc aaaaaaccgc tgcttgccct
    15901 gatgggtgcg ctgattatgg ttcagctgat gctggtcggc ggcgacaatt cgatggtgaa
    15961 aatcatcggt aaggaatttg ccgcaatggc gggcgaacac tgggtttatt tttcgccgta
    16021 tctgggcgcg atcggtgcgt tcttctccgg ctccaacacc gtgtccaacc tgaccttcgg
    16081 tccgattcag cagcaaatcg cccttgatac cggcctgtcc gtgaccctga ttctggcgtt
    16141 gcagtccgtc ggcggcgcga tgggcaatat ggtgtgcctc aacaacatca tcgccgtatg
    16201 taccgtattg gacgtgaaaa attccgaagg tgcgattatc aagaaaaccg ttatcccgat
    16261 ggcgatttac ggcgtgattg ccgtcgtcgc ggcaatgatt ttcttcctct aggcgaaacg
    16321 ccgccatcca tagcggcagc acgcggggaa cggaaaccgc cgctcgtccg ggcggcgcgt
    16381 cgggcagaat acccgtgccg gaacagcccg ccccgccaaa caaatgccgt ctgaaaccgg
    16441 aaaaggcttc agacggcatt tttatcgccg tttgcattca ggcgtagtaa tgatgcggca
    16501 tcttgccgtc aaggcggtag cgcgtgccgc aatacgggca ggcaacgctg cccgattctc
    16561 cttcgcccaa aggtaaaaac accctcggat gcccgttcca ctgctcgttg tccggtcccg
    16621 agcaatacag cggcagattt tccggcaaca cggaaatttc ctgcggattc agattgtcca
    16681 tttgattttc ctttgcgtgg ttggcgtgcg cccattttac gccatcggca ggctaaagga
    16741 tattttcggc gcaaagccgc aatccgctat aatccccact tttcagacgg cataccatga
    16801 ctgcgcttac ccttcctgaa gacatccgcc aacaagaacc atccgttctg ctctataccc
    16861 tcgtttccgc ctacctcgaa cacaccgccc aaaccggcga cgaatccctc tcctgcctgt
    16921 ccgacgacca gcacacgctg accgcattct gctacctcga cagccaagtc gaagaaggcg
    16981 gcttcgtaca gcttatcgcc tccggctacg gcgaatacat tttccgcaac ccgcttgccg
    17041 acagcctgcg ccgctggaaa atcaaagccg tgccgaaagt cttggacaaa gccaaagccc
    17101 tctacgaaca acacggcgaa accatcgaaa cgctcgccga cggaggcgca gacatcgaat
    17161 ccctgcgcaa acagttcccc gactttgaag aatgggacgg cgcatactac gaagccgccg
    17221 aacaagacct gcccctgctt gcagaacaca tcctgtcaaa ccgggcggca ttcgcccata
    17281 tcgggcaggc gtgattgcgt ctgtttccag cccgtgtaaa acagcgtaaa atcggcaaac
    17341 ccgaattatc ttccgtcccc atcaaggagc aagctatgtt cttcaagcac atcgaagccg
    17401 cccccgccga tccgattctc ggtttgggcg aagcgttcaa agccgaaacc cgccccgaaa
    17461 aagtcaacct cggcatcggc gtgtacaaag acgcatccgg cgcgacaccc attgtcaaag
    17521 ccgtcaaaga ggctgaaaaa cgcctgttgg aaagcgaaac caccaaaaat tacctgacca
    17581 tcgacggcgt tgccgactac aacgagcaaa cccaaatcct gctgttcggc aaagaccacg
    17641 aaatcatcgc cagccgccgc gccaaaacag cgcaaagcct tggcggtaca ggcgcattgc
    17701 gtattgcggc cgaatttgcc aaacgccagt tgaacgcgca aaccatctgg atttccaatc
    17761 cgacctggcc aaaccacaac gccattgcca aagcggtcgg tatccaagac aaaccttatc
    17821 gctactatga tgccgccaaa cactgcttgg attgggacgg catgattgaa gatttgaacc
    17881 aagcgcaaaa aggcgacatc gtcctgctgc acggctgctg ccacaaccct accggtatcg
    17941 accctacgcc cgaacaatgg gaaactttag caaaactttc tgccgaaaaa ggctggctgc
    18001 cgctgtttga ctttgcctac caaggctttg gcaacggttt ggaagaagat gcctatggct
    18061 tgcgcgtgtt cttgaaacac aatacagaat tgctgattgc cagctcttat tccaaaaact
    18121 tcggcatgta caacgagcgc gtcggcgcgt tcactttggt ggccgaagat gaagaaacag
    18181 cagcccgcgc ccacagccaa gtcaaaacca tcatccgtac cttgtattcc aacccggctt
    18241 cacatggtgc gaacaccatt gcactggtgt tgaaaaatga tgatttgaaa gcacaatgga
    18301 ttgccgaact cgatgaaatg cgcggccgca tcaaagccat gcgccaaaaa tttgtcgagt
    18361 tgctcaaggc caaaggttca acccaagact ttgattttat tattgaacaa aacggtatgt
    18421 tctctttcag cggcttgact cccgaacaag ttgaccgttt gaaaaacgag tttgccattt
    18481 atgccgtccg ttccggccgc atcaacgtcg ccggcattac cgacaacaac atcgattatc
    18541 tgtgtgaaag catcgtgaaa gtatagtcaa ttaaaatcaa aataggacag tagtgcatcg
    18601 tcaaatcggg cgtaatcaga caaaacggtt cgcagatacc gcttaatatt cgcccacact
    18661 ttctcaatcg ggttgggctc gggcgaacaa ggtgcgggag gcaatacctt atgt
//
LOCUS       DAWTHD010000029        18696 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWTHD010000029 DAWTHD010000000
VERSION     DAWTHD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18696
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.029"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..214)
                     /locus_tag="WM704_000798"
     CDS             complement(<1..214)
                     /locus_tag="WM704_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340259.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLV"
     gene            complement(276..2900)
                     /gene="alaS"
                     /locus_tag="WM704_000799"
     CDS             complement(276..2900)
                     /gene="alaS"
                     /locus_tag="WM704_000799"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFA9340260.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3104..4234
                     /locus_tag="WM704_000800"
     CDS             3104..4234
                     /locus_tag="WM704_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9340261.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4275..5177)
                     /locus_tag="WM704_000801"
     CDS             complement(4275..5177)
                     /locus_tag="WM704_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9340262.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5316..5813
                     /locus_tag="WM704_000802"
     CDS             5316..5813
                     /locus_tag="WM704_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFA9340263.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5875..6780)
                     /gene="mtrA"
                     /locus_tag="WM704_000803"
     CDS             complement(5875..6780)
                     /gene="mtrA"
                     /locus_tag="WM704_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFA9340264.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6909..7244
                     /locus_tag="WM704_000804"
     CDS             6909..7244
                     /locus_tag="WM704_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFA9340265.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7423..7845
                     /locus_tag="WM704_000805"
     CDS             7423..7845
                     /locus_tag="WM704_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340266.1"
                     /translation="MSAVFIAPYLTAFHEQEKIFEYADLTVTAPNRSGRAIKLEADGR
                     QYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7951..8514
                     /gene="pgsA"
                     /locus_tag="WM704_000806"
     CDS             7951..8514
                     /gene="pgsA"
                     /locus_tag="WM704_000806"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFA9340267.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVAAVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            8595..8670
                     /locus_tag="WM704_000807"
     tRNA            8595..8670
                     /locus_tag="WM704_000807"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8628..8630,aa:Gly,seq:gcc)
     gene            8696..8771
                     /locus_tag="WM704_000808"
     tRNA            8696..8771
                     /locus_tag="WM704_000808"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8729..8731,aa:Gly,seq:gcc)
     gene            8797..8872
                     /locus_tag="WM704_000809"
     tRNA            8797..8872
                     /locus_tag="WM704_000809"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8830..8832,aa:Gly,seq:gcc)
     gene            8898..8971
                     /locus_tag="WM704_000810"
     tRNA            8898..8971
                     /locus_tag="WM704_000810"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8930..8932,aa:Cys,seq:gca)
     gene            9072..9161
                     /locus_tag="WM704_000811"
     tRNA            9072..9161
                     /locus_tag="WM704_000811"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9106..9108,aa:Leu,seq:taa)
     gene            9265..10317
                     /gene="sohB"
                     /locus_tag="WM704_000812"
     CDS             9265..10317
                     /gene="sohB"
                     /locus_tag="WM704_000812"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFA9340268.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(10368..11222)
                     /locus_tag="WM704_000813"
     CDS             complement(10368..11222)
                     /locus_tag="WM704_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFA9340269.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFDERQLYRHYGLVSRLRVLISNREAFGYLCVGAAMG
                     ILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWS
                     ERNIKAGT"
     gene            complement(11233..11664)
                     /locus_tag="WM704_000814"
     CDS             complement(11233..11664)
                     /locus_tag="WM704_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFA9340270.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(11899..12768)
                     /locus_tag="WM704_000815"
     CDS             complement(11899..12768)
                     /locus_tag="WM704_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFA9340271.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(12844..13755)
                     /gene="hisB"
                     /locus_tag="WM704_000816"
     CDS             complement(12844..13755)
                     /gene="hisB"
                     /locus_tag="WM704_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFA9340272.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(13772..14851)
                     /locus_tag="WM704_000817"
     CDS             complement(13772..14851)
                     /locus_tag="WM704_000817"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFA9340273.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(14897..16186)
                     /gene="hisD"
                     /locus_tag="WM704_000818"
     CDS             complement(14897..16186)
                     /gene="hisD"
                     /locus_tag="WM704_000818"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFA9340274.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(16183..17070)
                     /locus_tag="WM704_000819"
     CDS             complement(16183..17070)
                     /locus_tag="WM704_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFA9340275.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(17162..17827)
                     /locus_tag="WM704_000820"
     CDS             complement(17162..17827)
                     /locus_tag="WM704_000820"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFA9340276.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(17906..18559)
                     /locus_tag="WM704_000821"
     CDS             complement(17906..18559)
                     /locus_tag="WM704_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFA9340277.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRALL"
BASE COUNT         4178 a         4574 c         5226 g         4718 t
ORIGIN      
        1 ataccaaact tgtatcaagg atgggcaatt ctatagttaa acctatttct tttgcgagaa
       61 tatttaattg tttgtcaatt aaccatcgac ggacaatagg agttgcaatg ctacgtacat
      121 atcttggagg aagagcatct acacgaatta gtgtttcttt taaagttgat atatcctctt
      181 ctaaaagttt ttgtaactct gttttagtat tcattctaac aatccaaaat ataagccatt
      241 tgaacaagac tcaaatggct tattaagatt taaatttaac ttaatttctg acaaagccaa
      301 ccttccgcgc ttgccagcat ttcgggcaat ttgtcggcat ccgtaccgcc ggcttgcgcc
      361 aagtcgggac ggccgccgcc tttgccgccg acttgttcgg ctgcaaattt aaccagatct
      421 cctgctttga cttttccggt cagggctttg gatacgccgg cgcacaggga gactttaccc
      481 tcgtttactg ccgctaaaag aatcacggcg ttgtcggatt ttccggtcag gtcggttacg
      541 gtttcgcgca gggcggctgc gtcggcttcg atttgtgcgg caacgagttt ggccgcgccc
      601 aggtcttttg cgtcgtccaa gagtttggcg cctgcgtgga cggcgagttc ggctttggcg
      661 cgtgccaatt ctttttccaa tgctttggcg tgtgccgcgc ctgcttggat tttcgccagt
      721 acgtcttttt cggtttgggc tttggtttcg gcaatgatgt cttttaccaa acgctcttgc
      781 tcttgcgccc atttgagtgc gttcaggccg gtgatggctt cgatacggcg tacgcctgcg
      841 gcaataccgc cttcgctgat gattttgaag aggccgatgt cgccggtgcg tgaaacgtgt
      901 gtgccgccgc acaattcggt agagaaaccg cccatttgca gtacgcgtac ttcgtcgccg
      961 tatttttcgc cgaagagcat catggcgtcg gttttttgcg cgtcttccat gctcataatg
     1021 gctgcgttga cggcaacgtt ggctaaaacg gcttcgttga cgcggcgttc gacttcagcg
     1081 atttcttcgg cggttaccgc ttggggatgg gaaatgtcga aacgggtgga ttcggcggta
     1141 accaaagagc ctttttgttc gacgtgtccg cccaatacgt cgcgcagggc tttgtgcatc
     1201 aggtgggtcg cgctgtggtt gcgcatattg gcattgcgga tttcgtcatc cactttggcg
     1261 gtaacgctgt cgccgacttt cagacggcct gaagtttgta cgccgaattg tccgaatacg
     1321 gccgctttga ttttttgggt atcgcgtact tcaaagcggt tttcgcctgc gaagatatag
     1381 ccgacgtcgc cgacttggcc gccggattct gcatagaacg gggtaaagtc gataacgacg
     1441 gcaccgctgt cgccttcgtt caattcgacg acttgcccgc cgtctttgta gagggcgagg
     1501 actttggatt cggtttggcg ttcgctataa cctttaaact cggtgtcttg accgtcgtaa
     1561 ggcagttggg cgttggcttt gaagctttgg gcggcgcgtg cgcgtgcgcg ttgggcttcc
     1621 atttcgcgct cgaagcctgc ttcgtccggt tcgatattgc gttcgcggca gatgtcggca
     1681 gtcaggtcgt atgggaagcc gtaggtatcg tagagtttga agataatttc gccgccgagt
     1741 gttttgccgc ctttggccag cgcgttttcc aacaaagcca taccggtttc cagagtttgg
     1801 gcaaaacggc tttcttcgtt tttcaaagct tcttcgattt gcgcctgttt ttctttcaat
     1861 tcaggatacg cgccgcccat ctcttgaacc aaatcggcaa cgagtttgtg gaagaacggt
     1921 ttgctttgac ccagtttgta accgtggcgc acggcgcggc ggatgatgcg gcgcaatacg
     1981 tagccgcgtc cttcgttgga aggcaatacg ccgtctgcaa tcaggaatga gcaggagcgg
     2041 atgtggtcgg cgataacttt caggctgggt tcttccatac tgaacggcgc gccggtttcg
     2101 cgggcaacgg ctttgagcag gtcttggaac aggtcgattt cgtagttgct gtggacgtgc
     2161 tgcatcacgg cggccatacg ttccaagccc ataccggtat cgacggacgg cttgggcagt
     2221 ggattcatat tgccttgttc gtcgcggttg aactgcatga atacgcagtt ccaaatttca
     2281 atccagcggt cgccgtcttc ttcggggctg ccgggaatgc cgccccagat ttctttgccg
     2341 tggtcgtaga aaatttcgga gcaggggccg caagggccgg tgtcgcccat ttgccagaag
     2401 ttgtcggacg cgtatttcgc gcctttgttg tcgccgatgc ggacgatgcg ttcagacggc
     2461 ataccgattt cgttcaacca gatgttgtag gcttcgtcgt cttctgcgta aacggtcgcc
     2521 aagagtttgt ctttggggat gttgagccat tcgggggaag tgagaaactc ccaagcgaag
     2581 tggatcgcgt cgcgtttgaa gtaatcgccg aaggagaagt tgcccatcat ttcaaagaag
     2641 gtgtggtggc gggcggtgta gccgacgttt tccaagtcgt tgtgcttgcc gcctgcgcgt
     2701 acacattttt gcgcggtggt ggcgcggctg tacgggcgtt tgtcgaaacc taaaaatacg
     2761 tctttaaact ggttcatgcc tgcgttggta aacagcaggg tcgggtcgtc gtgcggcacg
     2821 aggctggaag agcggacgac ggtgtggcct ttggtttcaa aaaattttag gaatttttgg
     2881 cgcagttcgg aggttttcat aattttttca atgtctctca aatgtcttgt catggtaaaa
     2941 gcagggaaaa cgaacggcgg tatattaccg caaatccctg tttctagcta tggaagcggc
     3001 ggctttcaga cggcattgcg ggattttgaa tgccgtctga agccctgtta ccaatatcgg
     3061 ctataatggc cgctttctcc aacccgatat gcaaggaatg ataatggtca aacatctgcc
     3121 actcgccgtc ctgactgctt tgctgcttgc agcgtgcggc ggttcggaca aaccgcctgc
     3181 cgaaaaaccg gcaccggcgg aaaaccaaaa cgtattgaaa atttataact ggtcggaata
     3241 cgtcgatccg gaaaccgttg ccgattttga aaagaaaaac ggcatcaagg ttacttatga
     3301 tgtgtacgac agtgatgaaa cgctggaaag caaggtgctg accggaaaat ccggttacga
     3361 cattgtcgcg ccgtccaatg cgtttgtggg caggcagatt aaggcaggtg cgtatcagaa
     3421 aatcgataag tcgatgattc ccaattataa acatctcaac cctgaaatga tgaggctgat
     3481 ggacggggtc gatcccgacc acgaatacgc cgtgccgttt tattggggga caaatacctt
     3541 cgccatcaat accgaacgcg tgaaaaaggc tttgggtacg gacaagctgc cggacaacca
     3601 gtgggatttg gtgttcaacc ccgaatacac gttcaaactc aaacaatgcg gcatcagcta
     3661 tttggacagc gcggcggaaa tttatcccat ggtgttgaac tatttgggta aaaacccgaa
     3721 cagcagcaat acggaagaca tcagggaggc aaccgccctg ctcaagaaaa accgccccaa
     3781 tatcaaacgc tttacttcgt ccggctttat cgatgatttg gcgcgcggcg atacctgcgt
     3841 aacaatcggt ttcggcggag atttgaacat cgccaaacgc cgtgccgaag aagcgggcgg
     3901 caaggaaaaa atccgcgtga tgatgccgaa agagggcgtg gggatttggg tggattcttt
     3961 cgtgattccg aaagatgcga aaaacgtcgc caacgcgcac aaatacatca acgacttcct
     4021 cgatccggaa gtgtcggcga aaaacggcaa tttcgttacc tacgcgcctt cgagcaagcc
     4081 ggcgcgcgat ttgatggagg acgaatttaa aaacgacaat acgattttcc cgagcgggga
     4141 agatttgaaa aacagcttta tcatggtgcc tatccggccg gcggcattga agtttatggt
     4201 gcgccagtgg caggatgtga aggcggggaa ataaagcccg atatgccgtc tgaaggatgt
     4261 tcggacggca ttttttatct ttggcggaag agggcttgca gccgctgttt gaaggcaatg
     4321 gggcggatgc tgctcaaaat gccgctgagg ataatgatgc acataccgag tatttcctgc
     4381 cagaaaagct cttcgcccag aaaaaatgcg gcagacaggg cggaaaagac gacggtcata
     4441 taggaaagcg aggcaaccgt gaatttgtcg ccgactttgt aggcgcgcgt catcgacagt
     4501 tgggcaatca gcgcggacac gccgatgccc gacagataaa ccgccgatgg aaaggacagg
     4561 gtgtgccagc cggtcagcgt cgcccaaacc gacgacatcg ccacgccggt tgcggaaagg
     4621 taaaagacga cgcgccagcc gggttcgccc gccaaagaca gttcgcgcac tttcaaatac
     4681 gcccagccgg acatcgcgcc gcccgccagc ccggcgagtg ccgccggttc ctgaccgctg
     4741 cggaacgagg gattaagcag caataccacg ccggcaaaac caaggagcag caccgcctgc
     4801 gtgtaaacgg aaatccgttc tttcaaaatc aggaaggaaa ataccgccaa aaaaatcgac
     4861 gaggtgtaac tcagggtaac gccggttgtc aaaggcagat gcgttaccgc gtaaaacagc
     4921 agcagcatcg cccccgtccc gaccatactg cggtttaagt ggtttttcca atggggcgtg
     4981 cggaaggtgt cgcgccgcaa tacggcggca gcaccgagcg taacggttga aaacagcatg
     5041 cgccaaaaga ccaattcgcc gctgccgagg gcaaattttg ccgatgcctc tttaatcaat
     5101 acgttcataa cggtgaagca ggccgccgcc accagcatcc agcccgatcc taaaatgtct
     5161 ttttttgcgg tatccataaa cggtcgttgc gataaggacg gtcggattgt aaaccttgcg
     5221 gcaaggcttg tggaatgtgt ttttgcctgc ttctgatgcc gaaattttat ttttcttgcc
     5281 gaacaatttg ttttctcaag gcaaacttga ttataatggc gggtatgaaa aaatacctta
     5341 tccctctttc cattgcggca gtcctttccg gctgccagtc tatttatgtg cccacattga
     5401 cggaaatccc cgtgaatcct atcaataccg tcaaaacgga agcacctgca aaaggttttc
     5461 gcctcgcccc ttcgcattgg gcggatgttg ccaaaatcag cgatgaagcg acgcgcttgg
     5521 gctatcaggt gggtatcggt aaaatgacca aggttcaggc ggcgcaatat ctgaacaact
     5581 tcagaaaacg cctggtcgga cgcaatgccg tcgatgacag tatgtatgaa atctacctgc
     5641 gttcggcggt agacagccag cgcggcgaaa tcaatacgga acagtccaag ctgtatatcg
     5701 agaatgcctt gcgcggctgg cagcagcgtt ggaaaaatat ggatgccaaa cccgataatc
     5761 ccgcatttac caactttttg atggaagtga tgaagatgca gcccttgaaa tgacgcggta
     5821 cgcaaatgcc gtctgaaagc tttttcagac ggcatttgcg tttgaagccc cgatttattt
     5881 ttgcccgcct tctttccggt attgccccgg cgaaacgtga tattgccgtt tgaacgcctt
     5941 gccgaaatgc gtttccgact gaaagcccac cgacagcgcg acctccaaaa ccgaatccgg
     6001 ggttttcttc agcagcaatg cgcctttttg caggcggata tggttcacaa aggcgtgcgg
     6061 gctgagtccg acttggcttt tgaagcggcg catcagttgc gcgcgcgaca tattggcggc
     6121 ggcaaccatt ttgtcaatat tccattcgtc ttccggtttg tctatcacct tttggatcaa
     6181 atgtcccaaa cgtttgtcct gccaaccttt caatacgccc gagagttcga catccttatc
     6241 ctgttcgaga taggcgcgca ggataagcac cagcaggacg gacggtaatg cgttgaccac
     6301 ggaaaccgtc cccgtcaaag gtttttcgct ttccagttgc agcattgaaa ccacatactg
     6361 caaacttgga tgggcaatgt tcagaaaaac ggtttccggc agcccgttca tcaaatcggc
     6421 gtgggtgtcg tagcggaaac gggcgcaaaa caggctcata tccagcccgt tgccgcactg
     6481 tttgaccata aatgtgccgt tttgccgtat gtccggttgt aaactttctc cgtattttcc
     6541 gtcgtggctc aacacatgac ccaagccgcg cgggaaaaat acaatatcgc ccgtgccgac
     6601 cggacgcggg gaagtttcgc cgtcgatgca gagataaccg ctgcccgccg taacaatgtg
     6661 taccagccct tcgcattgca aggtttcatg ccgtaccgac cattgtccgc ccaaaaggca
     6721 ctgcacatcc gcactgcccg tcaattgggc gagatcgacc agtttgtcca gaatgtccat
     6781 aaatcttttt gaaccataaa atgagatgat taaacgagaa acacaactga ataattgcaa
     6841 taatacgcac atcaaaaaca gatacgcaag cgcgtatcag ggttaaacgc aagagaaagg
     6901 aaaagaaaat gtttaaagat tggaaagaac ataccgcatt ggttaaaaaa tcattcggcg
     6961 agctgggtaa ggcgcatcct aaaatgctgc aagcctacgg cgcattggaa caggccgccg
     7021 ccgccgaagc actcgatgcc aaaacgcgcg aactgattgc catcgccgtt gccatcacca
     7081 cccgttgcga gagctgcatc agcgttcacg ccgccgccgc cgccaaagcc ggtgcgaccg
     7141 acagcgagat cgcaggtgca ttggcaaccg ccattgccct gaatgcgggt gctgcttaca
     7201 cttacgccct gcgtgcattg gaagcggttg aaacgcaaaa ataatccgtt tcggataaga
     7261 aatgccgtct gaaaatattt cagacggcat tttgcctgtt atgcttcccg atattttgca
     7321 gacaacatat gcagccgttt ttttcagacg gcatttgaga aacatggaac aagtatgaaa
     7381 tatcaccgct tggcattgtt tgccgccata agctgcctgt tgttgtccgc cgtttttatt
     7441 gcaccttacc tgacggcatt tcacgaacaa gaaaaaatat ttgaatatgc ggatttgacc
     7501 gttaccgccc ccaaccgcag cggacgggca atcaaattgg aggcggacgg gcggcagtac
     7561 cggctttcct gctacgggtt cgacagtttg tgcacaggcg gcaatatcgg cagagccatc
     7621 agggcgcggc aggttaagat tgttttaagc gaaaccgtcg gcaaaggttt tttaaacggc
     7681 gttctgttgg aataccgcaa cagtggcagt gtctatagca ataaagattt ttcccgcacg
     7741 gaagaccgcc ttgtcgaagt gttggcacaa cctgccgttt tcagcctgaa accgggtatt
     7801 ttgcttttat tgcccgccat tttcctgcgt ttgaaaaaaa tgtgaaaaca gattggcggg
     7861 gcgggggaat gactatgcgg caattttacg ttttgggtta tgaggataac cgttataatc
     7921 ataattttac agtccgcaca gagaaaaccg atgccttgga acatccccat cttcctcaca
     7981 tggttgaggg tcttgctcat tcccgtactg attgtccttt tctacctgcc gttctcatgg
     8041 ttttcagaag aagcagtcaa tgttgccgcc gccgtcattt ttgccgttgc cgccttgacc
     8101 gactggtttg acggattttt ggcaaggttg tggaaacaga cttccgattt cggcgcattc
     8161 ctcgaccccg tcgccgacaa gctgatggtt gccgtatcgc tgctgctgct ggtcaaactc
     8221 gaccggacct atgttttgtt cgcgatgatt atcatcggca gggaaattac catttccgca
     8281 ttgcgcgaat ggatggcgca aatgggaaaa aggagcagcg ttgccgtcgc cgccgtcggc
     8341 aagttcaaaa ccgccgcgca aatgctggcg attttctttt tgctgctgaa ttttcccgat
     8401 ttttacggat ttaatcttgt tgttatcggc aacatattga tgtttatcgc atctttgctg
     8461 acagtctggt cgatgctgta ttatctgaaa atggcgtgga aagaaattgc ctgaaaaaaa
     8521 cataaaaata gcttgacggt aaaaacataa tccataataa ttgcgtcttc ttcgatgtcg
     8581 gagagtgaaa tcgggcggga atagctcagt tggtagagcg caaccttgcc aaggttgagg
     8641 tcgcgagttc gagactcgtt tcccgctcca aaaatttcat tcttccgtaa tcattgcggg
     8701 aatagctcag ttggtagagc gcaaccttgc caaggttgag gtcgcgagtt cgaggctcgt
     8761 ttcccgctcc aaaaatttca ttcttccgta atcattgcgg gaatagctca gttggtagag
     8821 cgcaaccttg ccaaggttga ggtcgcgagt tcgagactcg tttcccgctc caagttttgt
     8881 agacaagcca gttttagggc gagatagcaa agtggttatg cagcggattg caaatccgtc
     8941 tacgccggtt cgattccgac tctcgcctcc atatatctta cggcggggtg gcagagtgtt
     9001 tatgctatga ggggtgcaac cttcatatag gccggttaaa atccgcgccc ccgcctccac
     9061 ctttcacaaa tgcccgggtg gtgaaatagg tagacacaac ggacttaaaa tccgtcggga
     9121 ctaaacatcc catgccggtt cgattccggc tccgggcacc aagctgaaaa tgacaatgcc
     9181 gctccaagcg gttatttttt tatctgtcgg atggggatgg acaatattga tatgttcatg
     9241 cctgaacaag aggaaatcca atcaatgtgg aaagaaattt tactgaatta cggtattttc
     9301 ctgctcgaac tgcttaccgt gttcggcgca attgcgctga ttgtgttggc tatcgtacag
     9361 agtaagaaac agtcggaaag cggcagtgtc gtactgacag atttttcgga aaattataaa
     9421 aaacagcggc aatcgtttga aacattcttt ttaagcgagg aagagacaaa acatcaggaa
     9481 aaaaaagaaa agaaaaagga aaaggcggaa gccaaagcag agaaaaagcg tttgaaggag
     9541 ggcggggaga aatctgccga aacgcaaaaa tcccgccttt ttgtgttgga ttttgacggc
     9601 gatttgtatg cacacgccgt agaatccttg cgtcatgaga ttacggcggt gcttttgatt
     9661 gccaagcctg aagatgaggt tctgctcaga ttggaaagtc cgggcggcgt ggttcacggt
     9721 tacggtttgg cggcttcgca gcttaggcgt ttgcgcgaac gcaatattcc gctgaccgtc
     9781 gccgtcgata aggtcgcggc aagcggcggc tatatgatgg cgtgtgtggc ggataaaatt
     9841 gtttccgctc cgtttgcgat tatcggttcg gtgggtgtgg tggcggaagt gccgaatatc
     9901 caccgcctgt tgaaaaaaca tgatattgat gtggatgtga tgacggcggg cgaatttaag
     9961 cgcacggtta cttttatggg tgaaaatacg gaaaagggca aacagaaatt ccggcaggaa
    10021 ctggaggaaa cgcatcagtt gttcaagcag tttgtcagtg aaaaccgccc cgggttggat
    10081 attgaaaaaa tagcgacggg cgagcattgg ttcggccggc aggcgttggc gttgaacttg
    10141 attgacgaga tttcgaccag tgatgatttg ttgttgaaag cgtttgaaaa caaacaggtt
    10201 atcgaagtga aatatcagga gaagcgaagc ctgatccagc gcattggttt gcaggcggaa
    10261 gcttccgttg aaaagttgtt tgccaaactt gtcaaccggc gagcggatgt gatgtagttt
    10321 gccgaacgga tggaaatgcc gtctgaagtg tgttcagacg gcatttttca agttccggct
    10381 ttgatgttcc gttccgacca ctctatccgt tgtccgatgt ctttcaaatt ggaatattgt
    10441 tcgaccaatc gcggcacatc gtccaaactc atggcaaaca tccacagata agtgccgacc
    10501 gaataaatat gccccgcgct gccgtagcct ttgagcgtca tcatcacaaa agcaaagccg
    10561 aacaaaatac ccatcgccgc gccgacgcag agatagccga aggcttcgcg gttggaaatc
    10621 agcacacgca ggcgcgaaac cagtccgtaa tggcggtaca gctgccgctc gtcgaagtgg
    10681 ttgtcgcgtt ccaagctgtt gttcaggcgg aaatacaggt tttcgctgat ggcggcaaaa
    10741 cgtggcaaaa gccataaaaa caacgcaagt atgcccaccg ccgacacgcc gacccaaaat
    10801 tccagcacca gcagcatgat gcacgcgccg aatatggata cgacggatgt cgcggcaatc
    10861 ggcaggtgtt cttcaaaaaa gctgacaaat tcacgcgaca gggcaacccg tgcagttacc
    10921 gctgaatgcg ggacttgccg ctgccgttgt tccaacacaa ccggcacggc gatttcggta
    10981 taaatccgcg taaacgtgcg cgtatcggca atccgccgtg ccgcaccgac cagccacatc
    11041 aaaaatacaa ccaaagcgta cagcaacgcc tgccacaccc tccccgcaat cacggcatta
    11101 atcgcccagc cgccaaacac cggatacccc agcatcaaaa ggttttccag tccgaccggg
    11161 gaaaatgtgc caatcaatcg cttgcggtgg gttttggcta tgtgtttcaa cattttccac
    11221 atatttgtcc ctctattttt tattttccga gattgttttt tccatcactt ccgccagtgc
    11281 atccaaatcg gcaaacagcc gggttgtgcg cttgtcgccg aatgtggaaa atactttgtc
    11341 gctgaattcc tgcgcgtttt ctgttaaagg tgcggcatgg actttgcctt tttcggtcag
    11401 cgacagcaac cgttcgcgcc tgtcctgttc gccttcctgc cattcaatca acccttgtcc
    11461 ggcaagggtt ttgcatacgc ctgaaacggt ctgtttgggc agactccact cttctccgat
    11521 gtgcttttgc gtgcggctgc cttcggttgc cagggtataa agtacggtaa agaggttgta
    11581 attcaaatcc tgctgcccga tccatttgtc aaagacattg caaatcaggt tgatacggat
    11641 tccaagttgg tcgagtcggt tcataattgg tcttgatatt gactaaattt gtgcggatta
    11701 tagtggatta acaaaaaccg gtacggcgtt gcctcgcctt gtcgtactat ttgtactgtc
    11761 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatagtcat aatcgggact
    11821 gatttcaagc gtggggcgat aaaaatgccg tctgaaacgc tttctggctt cagacggcat
    11881 attgtttgga aagggaagtc agtgttctgc caatttcagg taaacgccgg aaacgtcctg
    11941 ttcgccgtag ccggcttcaa ctgctttgcg gtagctggca gcaacggttt cgacggcggg
    12001 cagggtgttg cctgcctgtt caagctcttt gacggcgagg ttaaggtctt tggaagcgtg
    12061 tttgagtgca aaggcagggg ggaactcacg gtttgcccat agtgattttt ttgtttgaaa
    12121 cataggcgag tccattgccg agccgccgat ggcttcgacg atggtgtcgg tatcgatgcc
    12181 gaactgccgc gccatcagca tcgcttcgct gtacgcttcg ccgaaaatgc ctaagagcga
    12241 gttcaagacg agtttcgcgc ccgagccttt gccgacatcg ccgaaatgga aggttttttt
    12301 gccgacaagg gaaaatattt tttgcagcgg gtttaaaacg gcttcgctgc cgccgaacag
    12361 aatcagcagt gtgccgttgg tggcgggtcc gaccgatccg gaaacgggtg cttcggcaaa
    12421 ctgtccgcct gcggcttcga caagtgcttt gacggcgagg ttttcggtcg gggagatggt
    12481 gctcatgttg acgatgattt tgccggccaa tccgtcgcgg actccgttca ggatgtcgca
    12541 cacggcggca tagtcggaaa ccatcaggaa aatgacggga caggcgcgga cgagttcggc
    12601 ggtgctgccg taaacttttg ctcctttggc ggagatgggg gcagttttgt cgggcgagcg
    12661 gttgtatacg ccgacttcga tgccgccgtc caagagccgc gttaccatag gcagacccat
    12721 ttgccctaag cctacccagc cgatttgtgt gtatgtttct gcggacatag tgtttctcct
    12781 ttgttggggc gctgtaatgc cgtctgaagg cttcctgcgc ttcagacggt ctgtttgggg
    12841 atgttatgcg gtcagcgtgc ctttggtcga gggggtttgc ccagccattc tcgggtcgtg
    12901 ttcgactgcc atacgcaggg cgcgcccgaa ggctttgaat acggtttccg cctgatggtg
    12961 ggcgtttttg ccgctgaggt tgtcgatgtg cagggtcatc atgctgtggt tgacgatgcc
    13021 gtggaaaaat tcttcaaaca aatcgacatc gaaacgtccg attagtgcgc gggtaaattc
    13081 gatgttgtac acgagtccgg ggcggccgga aaggtcgatg acgacgcggc tgagggcttc
    13141 gtcgagcggg acgtaggaat gtccgtaacg gcggatgcct tttttgtcgc cgagtgcctg
    13201 ccggattgct tgtccgagtg tgatgccgat gtcttcggcg gtgtggtggt cgtcgatgtg
    13261 caggtcgcct ttgcagctga tgtcgatgtc aatcatgccg tggcgggcga tttggtcgat
    13321 catgtgttcg aggaagggaa cgccggtatc cagcctgctt ttgccgctgc cgtcgaggtt
    13381 gatggagacg gtgatttggg tttcgcaggt attgcggttg acggtaacgc tgcggcagcc
    13441 ggcagcagat gcggtttcgg caattcgggt ttcggcagtt ccggtgcttt ctgcggcggt
    13501 ttcggggaca gtgcgttcgc ggtgttttct gtcgagccag cctttgggtt tgccggtgtg
    13561 tttttcgagt tttgccatca ggctgggacg gatgccgcgc gcatcggggt cttctgcctg
    13621 cttttcaagg cgttgtttga gtttgtaaag tgcgacgggg gtacggtagc cgcagagttt
    13681 ggcaagcttg ggcagcgaac ctgcttcttg ggcgagagtc aggaagttgt tcaggtggag
    13741 ttgtgtcttg gtcataggga ttccgtttgg tttatcggta gagccggcgg ataacgtcga
    13801 ggacggcatc gttttgtgcg gagctgccta tggtaatgcg caggcagtgt tccaaaagcg
    13861 gatgtgcgcc gtgcagtttt ttgacgagga tgcggttttg tttgagtgtg tcaaacaaca
    13921 aatcggcatc gggtacgcgt atggtaatga agtttgcctg acttggaaag gcgttcagac
    13981 ggcatatttt gcccaattcg gcgaacatcc gttcgcgttc gtttttcagg ctgtcgatgt
    14041 tggcagaaat gatgccgtag tgttgcaggg cgagtttggc ggtggtcagg ctcagttggt
    14101 tcatattgta gggcggcagg attttttgca gttcgccgat gacttcggga cagcctgtcg
    14161 cataaccgat acgcagtccg gcaaaaccga ttttgctgag ggtgcgtaag actatcaggt
    14221 tgggaatcct gcccgcctgc ggcaggaagc tgtcgccgtt gaatgcgccg taggcttcgt
    14281 cgacgacgac gatgccgtct gaagcttcga tggcggcttc gatttcggca cgcgtgaaac
    14341 atacgccggt cgggttgttc gggtaggcga taaaggtcag ggcagggcgg tgcttcctga
    14401 cggcttcgag gacggcgggc aggttgaggg tgaaatctcc gttcagtgga acgccgacat
    14461 aatccatgcc gtacagcgcg gcgttgtggc ggtacatgat gaaaccgggt tcggctgcca
    14521 acattgccgc gccgggtttg gcggtcagca tggtgatgaa ctgtatcagt tcgtccgaac
    14581 cgttgcctaa ggcgacggcg gcgcagtcag ggatgtcgaa cgccgaacgc aatgcttcct
    14641 gcaaaccgca gccggagggg ttgggataga ggtggatggg ggcggatgcc agttgcgccc
    14701 gccattcccg catcagggct tcgtgcccct caaaaggatg ggcggggctt tccatcgcat
    14761 cgagtttggc aaaaccgggc ggaacatcgg tgattttgta tgcggacatg gcgaggatgt
    14821 cgttgcggat gaaggagcgg acggatttca tcgtgtttcc ttaatggttg gaatatgcct
    14881 gtacgccgtt tcggcattat ttcatacgga actctgccgc gcgtgcgtgg gcggtcaggc
    14941 tttcgccgtg tgccagcacg ctggcggttt cgcctaattt ttgcgcgccc tgttccgaaa
    15001 cctgaatcag gctggagcgt ttttggaaat catatgtccc caaaggcgag gaaaagcggg
    15061 cggttcggct ggtgggcaac acatggttcg ggccggcgca gtagtcgccg aggctttcgc
    15121 cggtgtagcg tcccatgaaa atcgcgccgg catggcggat ttttttcgcc cattcctgcg
    15181 ggttttcgac tgacagttcc aagtgttcgg gggaaatgta gttggagatt tcgcaggctt
    15241 cgttcaagtc tttaacgagt atcatcgcgc ccctgttgcc gagcgaggcc tcgatgatgt
    15301 cgcggcgcgg catggtttcg atgagcctgt ccatggcggc ttctacttcg tcgagatacg
    15361 cttgcgacgt gccgatgagg atggcttggg caatttcgtc gtgttcggcc tggctgaaca
    15421 aatccatcgc cacccaatcg gcgggtgtcg tgccgtcggc gatgaccagt atttcagacg
    15481 gccccgccac catgtcgatg ccgaccacgc cgaacacgcg gcgtttggcg gcggcgacga
    15541 aggcgttgcc cggaccggtg attttatcga cttgggggat ggtttccgtg ccgtaggcga
    15601 gggcggcgat ggcttgcgcg ccgccgacgg tgaagacttt ggttacgccg gcgacgtatg
    15661 cggcggcaag cacgatgtcg ttgcgttcgc ctttcggtgt cggtacgacc ataatgattt
    15721 ctttcacgcc tgcgacgtgg gcgggcatcg cgttcatgat gacggaactc ggatatgccg
    15781 ccttgccgcc tgggacgtaa atgccgacgc ggtcaagcgg ggtaatctgt tgccccagca
    15841 gcgtgccgtc ttcatcggtg tagctccacg attccatttt ttggcgttgg tggtagcttt
    15901 cgacacggcg ggcggcggtc tgcaatgctg tctgaacgtc gttcggaatg cgctcgaacg
    15961 ccgcgttcaa atcggcttgc gtgagtatta aatcatcgat gcttttagcg tttgtctgat
    16021 cgaatttgtt ggtgtattca atcaaagccg catcgccgcg cttttgcacg tcggcgcaaa
    16081 tgtcggcgac gatgcgttcg gtttcggggt tttgcgcggt ttcaaaagcc agcagggctt
    16141 tgagtccggc ttggaaatcg ggcgattggg tgttgagttt tttcatgatt tgggattctc
    16201 ctttgttatt ttgggacttg ggatcgtctg aagcgtctct tgctcggttt tgggtctgat
    16261 atacgctttt cctcccccgc taccaaactt caattcgggc aggcattttt gttgcagacc
    16321 gagtttggct aatacctctt cgctacgaga ggtagtacgg aaacggtaac gggtttgttt
    16381 tctgccgttt ttggtttcct cagtcattgt ttcaaaaccg tccacttcgt acgcactgac
    16441 aacagcgttt tgcagaccgg tgtgaacgcc gataacgtat ttcacttttg aagcaacatc
    16501 tttaccgata acccagttgc ccaacgtacg cgatttgagg ttggcatcgt cttggttgtc
    16561 gaaaaggtag tctaattctt cgtcagtatc taaatcgaaa gcattgtgga tcttgatggc
    16621 gagaatcagc ccgtcggggt taatctcgct aagtgggaga gaagagaatc caaagcggcg
    16681 atctagttct tctacgctaa taccacccgg accatgcccg gcaattttat ttttgagttt
    16741 tttacccaag acagatttaa caaaatgaat taaggcagat tcggcggcga gcgcttcgac
    16801 ttcagtcaga tgatagctga tgatatagcg accgagtttc ttgcatttgg agatggcttt
    16861 gagtttccga tcgataatct cgccggagac tgggtcttgt gaacgactag caacccattc
    16921 atgctcgaag atacgattac cgcaaccttt tccgatgtag aggattttgt cttttttcag
    16981 gtcggtcaga caatagacat aaaaccgacg ctcgccgtta ttcaaaacgg aaagggtcga
    17041 atctgagaat ttttttattt ttgcaaccat tttaacttct cctatacagt ttactgccgg
    17101 tgatattttc agacggcctg tcggcgggaa cggatagggt cgtctgaaaa cgcatcttta
    17161 ttcaaatgaa tgcccacttc gccttcactg cgccgccgaa cgactgaata atcggctcca
    17221 gcagcgcgta tttcgttttc aaagcagcct tgttgaccac caggtagctg gaaatgtcga
    17281 cgatgtgttc gactgcctcc aagccgtttg ccttcaaggt gtttcccgtc gaaaccaagt
    17341 ccacaatcgc atcgctcaag ccgaccagcg gcgcaagttc catcgagccg tacagtttga
    17401 taatgtccac atggacaccc ttgccggcaa aatgagatgc cgcgatttca ggatacttcg
    17461 tggcaatctt cagacggcat ccgggttgcg aagcggcttc gtaatcaaac cctttacgca
    17521 cagcaaccat catgcggcac ttggcaatct ccaaatccaa aggccggtaa agccccgtgc
    17581 cgccgtgttc gatcagcacg tctttgcccg caatgccgaa gtccgccgcg ccgtagcgga
    17641 cataagtcgg cacatcggtt gcgcggacaa tgacaaggcg gatgttttca tggttcgtcc
    17701 cgataatcag cttgcgcgat ttttcgggct cttcagtcgg agcaatgccg gcagcggcaa
    17761 gcagcggcag cgtctcttca aaaatgcgcc ctttggataa ggcgatggtc aaagcattat
    17821 cctgcatggt gtggatgtcc tttgtcggtt gaaacaataa tgccgtctga acaggcaccg
    17881 gcggtgcaga cggcatacgg ttttatcaga gcagggccct tacatcggca gcaatcgttt
    17941 ccggctcgct tccgtaaggc gagaaaatgg caacctcacc gtttttgtcg ataagatacg
    18001 caccggaaga gtggtcgacc aaatagtttt cgctgtcgtc tttttgattg attttggcag
    18061 aaaccacgcg gtattgctgc ttgatgaccg gcaggttttg gccgcccgtt gccgtcagac
    18121 cgataaagtc cggattgaac tgtttggcat acttgccgat gatttcaggc gtgtcgcgtt
    18181 ccggatcgat gctgacgaac accactttca cgtccttagc ctgcccgccc aactgcttca
    18241 aagtgtcgct gtacgtcaaa agctctgtcg ggcagacatc gggacagtgc gtaaagccga
    18301 aagacagaat cacgaccttg cctttcaaat cgctcaggct gaaaggcttg ccttcgccgt
    18361 cggtcagcgt gaaatcgccg ccgatgtctt ccttacgcat atccgtaccg cgcgtttgcg
    18421 gctttgccgc attttccgca gccggcgcgg atgcacttga agaagcggct tgcgccgcac
    18481 tgttgtcttg aggtttgcag gcggcaagcg cggcaagtac gaaaacgccc ggcaaaaagg
    18541 aacgcggtac ggaaaacatg acaatctccc aagaataaat agaaaaacct gattcgtacg
    18601 gaatttgaat atttccatct taacgcgttt ggcagattta atgtagtatt tttttacgtt
    18661 gtccgtacgg caggaattgc cgaatcatac agaatc
//
LOCUS       DAWTHD010000030        18500 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWTHD010000030 DAWTHD010000000
VERSION     DAWTHD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18500
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.030"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            29..241
                     /locus_tag="WM704_000822"
     CDS             29..241
                     /locus_tag="WM704_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9340278.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            426..940
                     /locus_tag="WM704_000823"
                     /pseudo
     CDS             426..940
                     /locus_tag="WM704_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            937..1263
                     /locus_tag="WM704_000824"
     CDS             937..1263
                     /locus_tag="WM704_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFA9340279.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1284..2546
                     /locus_tag="WM704_000825"
     CDS             1284..2546
                     /locus_tag="WM704_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFA9340280.1"
                     /translation="MGNSRLSQLPAPAAIEETDFEGIFARKKAALTALCPESIRETVA
                     QTLELESEPLTIDLQQQAYQELLVRNRINEAVKANLLAYAQGSDLDHIAAQYGLSRKT
                     IRAADPDANPPVAAEYETDDAFRARVQAHPEKYAAGPRTAYEAHAIDAHPQITHARAV
                     RRAAGTVEVYIKTQSGTPDETILTAAREYLSAETRRPLCDNVQVTAAQPKDAAVEYSA
                     EYHPAANIQAERQTACEALDNLWRQNAHIGASVALSKIIGALDTPGVKKNHTAQPRCR
                     HRMRQRRIHPNHVHARARINEQHRPREQQPPATRTGKADRTRNCRRLPPTRPRPMRPR
                     IFTLSRLRKKHRHGRGLGLCRNRRSPPQPHRRLCRNPRPKRHAVRHPRPLPHLAAGRN
                     PNYRTRRRVQVGRLGLVRRQPHIRQARG"
     gene            2506..2733
                     /locus_tag="WM704_000826"
     CDS             2506..2733
                     /locus_tag="WM704_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340281.1"
                     /translation="MFDGSRTFGRREGDWAEYRIVLTRPVSIRQTARIRAMLAEIAPL
                     RCELTALDYRNHPHRWNGKIRFNGEYGFGTT"
     gene            2773..3342
                     /locus_tag="WM704_000827"
     CDS             2773..3342
                     /locus_tag="WM704_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFA9340282.1"
                     /translation="MANATEQNQFDQAVRLIEPGDSVVVGPGAPVNQPLQALANRTLL
                     LKNQTEALQTASDTKAAASTAVNAGDGLTGGGSLAQSRTIALGAPGQITATSQNTVPK
                     NGHTHAIDTARTDRAGIVRLDNAISEAEDTAATPKAVKTALDQARAAAATADLKVSLS
                     DNQTVTGQKTFTAETQFQSGIRLSANPTH"
     gene            3774..5024
                     /locus_tag="WM704_000828"
     CDS             3774..5024
                     /locus_tag="WM704_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFA9340283.1"
                     /translation="MEKTQFGRHPRRRDRVLSQSRPKPRRLSQSRRHDLCTKHLLDLY
                     RALGNTNKLPDLTRTDIGITAWFPSDQIQTGWLAFDDIRERVTESAYPELYRLLTEKY
                     GRIQNVPQAEDRFIRNAGNGLAVGTKQEDEIKRHVHKVFSHWANHPDAAAVGYEDRNE
                     RQRSALVSTWTDGDLNDNGFLTPRLDSKMATGGAENRPKALVLKLCIKAADTLGEAVF
                     RIKSHGETANVGALDASRLAQGLQEKADRDHTHTTAQIQGLDEKISAAVAAQFTRQTI
                     GGVDIVRFPDGTMIQTGSYRFARGGSPIGNEVVFPIAFADGNVKCFVSERHSGRVNGE
                     RQHNWLFIRAKNHAAAIITNWYESSCDWMAIGKSASGNAASPTPIVPEIPEIDEEPQR
                     ESGRSSTGLRNPRRHRGLDFPVGS"
     gene            5123..5452
                     /locus_tag="WM704_000829"
     CDS             5123..5452
                     /locus_tag="WM704_000829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340284.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5468..5779)
                     /locus_tag="WM704_000830"
     CDS             complement(5468..5779)
                     /locus_tag="WM704_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HFA9340285.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5787..6069)
                     /locus_tag="WM704_000831"
                     /pseudo
     CDS             complement(5787..6069)
                     /locus_tag="WM704_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6151..6764
                     /locus_tag="WM704_000832"
                     /pseudo
     CDS             6151..6764
                     /locus_tag="WM704_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6915..7172)
                     /locus_tag="WM704_000833"
     CDS             complement(6915..7172)
                     /locus_tag="WM704_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340286.1"
                     /translation="MPSECGAAAILPRIPFIGGAALVEPRVVLPAFQLADIRLPAVVA
                     VEKYAVDQGIYSGLTKTGTALPRLAVLFVLSAASSPCPDFC"
     gene            complement(7226..7609)
                     /locus_tag="WM704_000834"
     CDS             complement(7226..7609)
                     /locus_tag="WM704_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFA9340287.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGNTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(7714..7854)
                     /locus_tag="WM704_000835"
     CDS             complement(7714..7854)
                     /locus_tag="WM704_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340288.1"
                     /translation="MPQYRNWQSGLDAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            complement(7899..8351)
                     /locus_tag="WM704_000836"
     CDS             complement(7899..8351)
                     /locus_tag="WM704_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFA9340289.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            complement(9129..10775)
                     /gene="pgi"
                     /locus_tag="WM704_000837"
     CDS             complement(9129..10775)
                     /gene="pgi"
                     /locus_tag="WM704_000837"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFA9340290.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDRHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            complement(11001..11849)
                     /gene="hexR"
                     /locus_tag="WM704_000838"
     CDS             complement(11001..11849)
                     /gene="hexR"
                     /locus_tag="WM704_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFA9340291.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(11898..12884)
                     /locus_tag="WM704_000839"
     CDS             complement(11898..12884)
                     /locus_tag="WM704_000839"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFA9340292.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            complement(12865..13560)
                     /gene="pgl"
                     /locus_tag="WM704_000840"
     CDS             complement(12865..13560)
                     /gene="pgl"
                     /locus_tag="WM704_000840"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFA9340293.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            complement(13711..15156)
                     /gene="zwf"
                     /locus_tag="WM704_000841"
     CDS             complement(13711..15156)
                     /gene="zwf"
                     /locus_tag="WM704_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFA9340294.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            15474..15592
                     /locus_tag="WM704_000842"
                     /pseudo
     CDS             15474..15592
                     /locus_tag="WM704_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            15676..17511
                     /gene="edd"
                     /locus_tag="WM704_000843"
     CDS             15676..17511
                     /gene="edd"
                     /locus_tag="WM704_000843"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFA9340295.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            17721..18359
                     /locus_tag="WM704_000844"
     CDS             17721..18359
                     /locus_tag="WM704_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFA9340296.1"
                     /translation="MSKLTPRQILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
BASE COUNT         4416 a         5629 c         4711 g         3744 t
ORIGIN      
        1 tatgcgaaac aaaataaacc gcaacgacat ggaactcggc tacacgcctt acaaccttcg
       61 caccctgcgc aaccgctgca aactgacaca ggccgagttg gcgcaaatcg tcggggtgaa
      121 gcactacata caggtaggcc gctgggaagc cgaacccgat accgaaacgc ggcgggccga
      181 tatgccgctg gaaaaatggc ggcagttcct cgattggata gaaaaaacaa acgccgtctg
      241 aaaccttcag acggcatttt ttacccaccc ccaaacctaa aaaccacaac caagccctac
      301 accgcttgcc cccacctcgc cgccgcccca aaataacctc actctgttgg aagcctgccg
      361 gaaacggcaa aagccgcccc gttccgccat tacggggcgg tttcccacat caagacgaga
      421 cagaaatgac cgaccgccaa atagacaacc tcatcaaacc cgccaccatc gccgacaccg
      481 accccgcgtc cgcgcacaac acggcggcat taccaccgac tggctgccct atatcgtccc
      541 ctttgcaggc ggcgtgtcag tatggcgcat cccaagtgtc ggcgaagcct gcaccatctt
      601 atcccctgcc ggcgaacccg agaacggcgt agttttatgc tgccaagcct ccgaccgata
      661 ccccgccccg tccgccgacc ccgccgaaac cgtcgtccga tttcccgacg gcacgcacat
      721 ccgctacaac cacaacagcg gcgcgatgga attaaaagcc gttacaagcc tgaccatcga
      781 caccccccaa accaccataa ccggacacct gaccgtcaac caaaccacca ccgcccaagg
      841 gctgctgacc taccaaaatg gcatgaacgg ccaaggcggc agcctgtccg agcacaccca
      901 ccccgacgac tccggcggta caacggaaaa accccaatga ccgacgcaga aaacggacgc
      961 gggcaagaca ccctcgccca catcgcccaa tccatccgca acatcctgtt cacaagaata
     1021 ggaacgcggc tgatgcgcga agaatacggc agttttattc ccgacttgat cgatatgccc
     1081 gccggtcacg ccgccatcgc cctcatccac caagccgccg ttaccgccct cgcaagatgg
     1141 aaaccccgca taaccgtccg ccgcatacag gcagacaccg ccgacctcgc cgccggcaaa
     1201 atcaaattaa ccctagacgt aacccttgcc gacggcggcg aacgaaccta ccgcatcaaa
     1261 taataaaaac aacaggataa acaatgggaa acagccgatt aagccaactc cctgcgcccg
     1321 ccgccatcga agaaaccgac tttgagggca tcttcgcgcg caaaaaagcc gccctcaccg
     1381 ccctatgtcc cgaaagcatc cgcgaaaccg tcgcccaaac cctcgaatta gaatcagaac
     1441 ccctaaccat cgacctgcaa cagcaagcct atcaagagct gctcgtccgc aaccgcatca
     1501 acgaagccgt caaagccaac ctcttggcat acgcacaagg cagcgacctc gaccacatcg
     1561 ccgcccaata cggactttca cgcaaaacca tccgcgccgc cgaccccgac gcgaacccgc
     1621 cggttgccgc cgaatacgaa accgacgacg cattccgcgc ccgcgtccaa gcccatcccg
     1681 aaaaatacgc cgccgggccg cgcaccgcat acgaagccca cgccatcgac gcacaccccc
     1741 aaatcacaca cgcccgcgcc gtgcgccgcg ccgccggcac ggtggaggtt tacatcaaaa
     1801 cccaaagcgg cacgcccgac gaaaccattt tgaccgccgc gcgcgaatac ctgtccgccg
     1861 aaacacgccg ccccctttgc gacaacgtgc aagtaaccgc cgcccaaccc aaagacgcgg
     1921 cagtagagta ttccgccgaa taccaccccg ccgccaatat tcaagccgaa cggcagaccg
     1981 cctgcgaagc cttggataac ctatggcggc aaaacgccca catcggcgca tccgtcgccc
     2041 tgtctaaaat catcggcgcg ttagacaccc ccggcgtgaa aaaaaatcac actgcacagc
     2101 cccgctgccg acatcgaatg cggcaacggc gaatacatcc gaatcacgtc cacgctcgag
     2161 cgcgaataaa tgaacagcac cgtccccgcg aacaacagcc ccctgcaaca cgcactggca
     2221 aagctgacag aacgcgaaac tgccgccgtc tcccgccaac tcgaccccgc ccgatgcgac
     2281 cccggatttt taccctttca cgccttcgca agaagcatcg gcacggaaga gggctgggac
     2341 tttgccgaaa ccgacgaagc ccgccgcaac ctcatcgcag gctttgccga aatccacgcc
     2401 cgaaaaggca cgccgtacgc catccgcgcc ctcttcccca tcttgcggct gggcgaaatc
     2461 caaattatcg aacgcgacgg cgagttcaag tgggacggct cggtcttgtt cgacggcagc
     2521 cgcacattcg gcaggcgcga gggtgactgg gcggaatacc gcattgtctt aacgcgcccc
     2581 gtcagcatcc gccaaaccgc ccgcatccgc gccatgttgg cggaaatcgc ccccttgcgg
     2641 tgcgaactta ccgcgctcga ctaccgcaac catccccacc gctggaacgg caaaatccgc
     2701 tttaacggcg aatacggttt cggcacgaca taacgcgccc ccgaaaatca acaaacaaaa
     2761 ggaagcccca aaatggcaaa cgcaaccgaa caaaaccaat tcgaccaagc cgtccgcctc
     2821 atcgaacccg gcgacagcgt cgtcgtcggc ccaggcgcgc ccgtcaacca accactgcaa
     2881 gccctcgcca accgcaccct tttgctcaaa aaccaaaccg aagcccttca gacggcatcc
     2941 gacaccaaag ccgccgcaag caccgccgtc aacgcaggcg acggactgac gggcggcggc
     3001 agccttgccc aaagccgcac catcgcactg ggcgcgcccg ggcaaatcac cgcaacaagc
     3061 caaaacaccg tcccaaaaaa cggccacacc cacgccatcg acaccgcacg caccgaccgc
     3121 gccggcatcg tccgattaga caatgccatt agcgaagccg aagacaccgc cgccaccccc
     3181 aaagccgtca aaaccgcgct cgaccaagcc cgcgccgccg ccgcgaccgc cgacctcaaa
     3241 gtctccctct ccgacaacca aaccgttacc ggccaaaaaa cctttaccgc cgaaacacaa
     3301 ttccaaagcg gcatccggct gtccgccaac ccgacgcact gaaacggcgg ctacaaagcc
     3361 tatatcggcg cggataacga aaacgcccac atcgtcttcg gcgacgacac cctgcgcctg
     3421 cacggcgcaa ataaccgcat ttcctacaac aaccacgaca tcttccacaa agccaacaaa
     3481 ccgcgtttca acgaagacat cgaaggcaaa ccgaacacgc tgtccggcta cggcatcggc
     3541 aacttcaaag tcgaaacatt ccggggcgat ttgaacagcc tcaaaaccga cggcgtctat
     3601 tccctgccga cggcggtcgg cagctcgaac ctgcccgttg aaaacaccgc ctgccatatc
     3661 caagtcatcg ccggcacgca acccggctgg tgcaggcagt tgggctatcc cgcctacacg
     3721 tccgacgtgt acgaacgcta ccagaccagc agcgcaaacg acgactggag cgcgtggaaa
     3781 aaactcaatt cggaaggcat ccccgccggc gcgatcgtgt cctttcccaa agccgtccga
     3841 aaccccgcag gctatctcaa agccgacggc acgatctttg cacaaaacac cttctcgacc
     3901 tttaccgcgc cctgggcaac acaaacaaac tgcccgacct gacccgtacc gacatcggca
     3961 tcaccgcgtg gtttccgtcc gaccaaatcc agacgggctg gctggcgttt gacgacatcc
     4021 gagagcgcgt aaccgaatcg gcttatcccg aactttaccg cctgctgacc gaaaaatacg
     4081 gacgcatcca aaacgtcccg caggcggaag accgctttat ccgcaacgcg ggcaacggct
     4141 tggcggtcgg aacgaagcag gaagacgaaa tcaaacggca cgtccacaaa gtattttcac
     4201 actgggcaaa ccacccagac gccgccgccg tcggttacga agaccgcaat gaaaggcaaa
     4261 gaagcgcgct cgtatcgact tggacggacg gcgacttaaa cgacaacggc tttttaaccc
     4321 cgcgattgga cagcaaaatg gcaacgggcg gcgcagaaaa ccgccccaaa gccctggttt
     4381 taaaactgtg catcaaagcc gccgacacct tgggcgaagc cgtgttccgg ataaagtccc
     4441 acggcgaaac cgccaacgtc ggcgcactgg acgcgtcaag gctggcgcaa ggtttgcagg
     4501 aaaaagccga ccgcgaccac acccacacca ccgcccaaat ccaagggctg gacgaaaaaa
     4561 tcagcgccgc cgttgccgcg caattcacac gccaaaccat cggcggcgtg gatattgtca
     4621 gattccccga cggcacaatg atacagaccg gcagttacag gtttgcaaga ggcggcagcc
     4681 ccataggaaa cgaagttgtc ttccccatcg cctttgccga cggcaacgtc aaatgcttcg
     4741 tatccgaacg ccattcggga cgcgtcaacg gcgaaaggca acacaactgg ctgtttatcc
     4801 gcgcaaaaaa ccacgccgcc gccattatta ccaactggta cgaaagcagt tgcgactgga
     4861 tggccatcgg caaatccgcc tcgggaaacg ccgccagccc caccccgata gtccccgaaa
     4921 tacctgaaat cgatgaagaa ccgcaaagag agagtggaag atcatcaacc ggactccgaa
     4981 acccccgccg ccaccgaggc ctcgacttcc ccgtggggtc gtaagactag cgggcggcag
     5041 ggcaggctgt agagacgggc ttcagcccac cgatccaagc aatccgaccg aaaccggccg
     5101 cgccgccaat cctgcgaaac ctatgccccg ccaatcctgc cactcttcgc cattcccgcg
     5161 aaagcgggaa tccggaaccc aaacgcggca ggaatctatc ggaaagaata acccgaccgc
     5221 cgtcattccc gcgcaggctg gaatccagac ccacaacgtt aaggcggttt atcagaaaaa
     5281 accgaaaccg aacgccctag atttccgcct gcgcgggaat gacgaagagt tggggagtga
     5341 cgaaaggcgg gaacaaccgc gcaaaaagcc gccgactcct tcagacggca tcggcaataa
     5401 aaaccgcacg gccgaaaccg cgcgggaaag gatagccggg cgcgcccgat aagcagcggc
     5461 cgcagggtta agtccttgca cctgttttca tcgaagacaa aggcggttcg ggaaataagg
     5521 gcgcaatatc aaaagttttg cgttgcctga tttgccacaa atcataaccg gcctgcatat
     5581 tcagccaaaa atcaggcgaa gtgttgagca gttggcttaa acgaaccgcc atctcgggcg
     5641 taatcgaaga ttgggcgttg acaatgcgcg ataaggcaac ccggctgaca cccaggcgtt
     5701 tcgctatatc ggtaatgctt gcgccgttaa tatactcttt taaaacaagg cccgggtggg
     5761 caggattgtg cattttcata ttattcctag tgataatcct gataatcgac aacttcgaca
     5821 ttgccgtcgt tgaaacgaaa agtcaaacgc caatttccgt tgactttgaa ccgcccaatg
     5881 tcccgacaaa ctgcccttga ggggatgaag attccaactc ggcacagcca tatccgaagg
     5941 cgtttgcgcg gcattcagcg cggttagaag cagattcagt ttgacggaat gcccggcttg
     6001 gatgcccgat agcgagcctg ttttaaaaaa gcgttcaagc cctttgtgtt tgaaagaaac
     6061 aatcatcatc ccaccccgtc tgtatcgcca aaggatacaa aacttttatt tgattgcaaa
     6121 gcgacgatat acaaaaaagg aaaccccgaa atgaccatct atttcaaaaa cggcttttac
     6181 gacgacacat tgggcagcgt ccccgaaggc gcggttgccg tcggcggcga agagtacgcc
     6241 gccctcttcg caggacaggc gcagggcggg caaattgccg ccgattccga cggccgcccc
     6301 gttttaaccc cgccgcgccc gtccgagtac cacgaatggg acggcaaaaa atggaaaatc
     6361 agcaaagccg ccgcccgttt cgccaactcc cgtttcgcca aacaaaaaac cgccttggca
     6421 ttcagcctcg cggaaaaggc ggacgaactc aaaacagcct cttggcgggc tatccccaag
     6481 tggaaatcga cagcttttac aggcaggaaa aagaaaccct cgcgtggcag gcggacaacg
     6541 acgccccgac cccgatgctg gcgcaaatcg ccgccgcaag gggcatagaa ttggacgttt
     6601 tgattggaaa ggttgtcgaa aaacccgccc gcctggccgt tgccgtcgac gcgattatcg
     6661 gaaagcggca gcaacttgaa gatagattaa ataccatcga aaccgcgcca gcattagatg
     6721 cgttagaaag ggaaatcaaa gaatgggcgc taaacgtcgg ctgagagaat atatttatca
     6781 tctactagtg gccatagacc aactgttcaa cgctataacc ggcggcgcgg cagacgaaac
     6841 cctctcaagt cgcacctatc gcggcgcgcg gctcgcccaa caacccaaaa cccgctggaa
     6901 gattttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
     6961 agtacggcaa ggcgaggtaa cgccgtaccg gtttttgtta atccactata tataccctga
     7021 tcaacggcgt atttttcgac cgccaccact gccggcaggc gtatatcagc taactgaaag
     7081 gcaggcagca cgacgcgcgg ttcaaccaaa gccgcgccgc caatgaaagg gatacgcggt
     7141 aaaattgccg ccgcgccgca ttcagacggc atcggcaata aaaaagcccg aaaaaatcgg
     7201 gctgaaagtt aaggctgcat cgcctttaag gcggctttcg ccaaaaaacc gctcgggttt
     7261 catgacgggc ggcagtgcag gcatcgacac ggttgaggat atgttgcggc caactgacgt
     7321 taaaccgcac ctgtccggtt aatgcggcat cgtccgcttc aaccatcgtc cacaacaccg
     7381 catcggcata atcgggattg tcccgattaa cctctatgct ttgcgcctcg ggaatcggca
     7441 ggttttcctg caaaagccct tcgagatgga acattgcggc ggctttggca tcggcaagcg
     7501 cgtcggcaac cgtattgccg acaggatagc agcccggcag atcgggaata atcacgcccc
     7561 agttgctttg atcggggtct tggaacagca cggcgggata gtaaatcatt acttcctttc
     7621 tcggatatgc ggtatcggca aagtccaaac gcaacgaagg ggatttaaaa acgacaaaaa
     7681 cgcaaaagcc gccgacattc ccgcatccaa gtttcagtca atcagataac cttggatttc
     7741 tttggttttc gcattgattt ctctggtacg gcagtcagat tgtgccaccc cgtattcgtc
     7801 gccgtcggcg catttggcat ccaaaccgct ttgccagttg cggtactggg gcataacggt
     7861 ttcggcaata tcggcgggca gtgcctgaac cgcgctgttc agagccgctt tggcagttct
     7921 ttcttgtcct cttccctgac catttcgtcg ataagggctt ttttacggtt gtgcagctct
     7981 tccaagcgtg cttcggtttc tgccgtttta cacgccaact cactgacttt tatgccgttt
     8041 ggcgtttcac cttcggcttt cgcattgttg gcacagatct tgtccatacc gcttttccat
     8101 gttttctgcg aggcttgcag cttattctgt acagtttgag gcaatccttt ccaaaactgc
     8161 tcgaactcgc tgcgcgacag cttgtagcgt tcttcccatt cggatacgcg ggcggcttcc
     8221 tgctcccgac ccaatgcctc ctgcgccgcc gcttcttctg cctccctcgc ttcctgcgcg
     8281 gtggcttctg ccgcttcaag tttttcgttc ctttgtttcg ctttgtccaa cggctcttta
     8341 atcagtgcca tagacaccag ctcgctgagc ggatggatga tatcgtgtgc ctgactgagt
     8401 tcggcaaaga ttttgctttt gtcgtctgtc ggctggacgc tgtaagaaat agttttgaca
     8461 taatacgcgc cttctttgcg gtagtagggt tcaaaaaagt cgggcgtttt cttatagctg
     8521 ttgcctatag attggttggc ggcgacggca taatcgacaa catcgtcggg cacgtccagt
     8581 tttaacgcgg cttcacattt gagtttgctg gacgtgtcgg ttttctcggt agttcggact
     8641 tcatcgacgg ttatgcccaa acgttcgacc attctgcgga tatgcgatat gccgatatcg
     8701 gcaaatgctt ggtctttgaa tgcgtcgtcg tcaaaagttt tgacggtttc accttcgaca
     8761 ttgtcgcgga ccagtttgac caaaatctct ttagtctctt cgcttccgca ggaaatttcc
     8821 tttccggaaa acgggttgcc tattcccaaa cggtcgcagc ccgacaaaat cagcatactt
     8881 gccaatgcca gtgcgggtaa attttttttc attagatttc ctttgccgtc caaacacgga
     8941 ctgtctgttg cgtatttcca cacacgctac ggcacttgaa gtacatggct gcggaacata
     9001 tatgtttaaa tttatgcaaa ccgccatatc gcgattgcac acgccctgca aatttaccga
     9061 cggttttcct gttcgataaa aatgccgtct gaaacggtcg gcgttcagac ggcatttttc
     9121 cgcaggtttt atttgcggtt ggtctgcagg tagaggttaa tcaggcgttc ggtcgagctg
     9181 tcgtgttttt gaggttcggt ttcgcctgtc agttcgccca aaatggtttt agccagttgt
     9241 ttgccgagtt ccacgcccca ctggtcgaag ctgttgatgc cccaaatgat gccttggacg
     9301 aaggttttgt gttcgtacat ggcaatcagg ctgcccatat tgcgggggtt gaccttatcc
     9361 atcaggatga ggttggtcgg gcggttgccg gagaaggttt tgtgcggaac cagctcttcg
     9421 atgcgcgcct catccatacc ctgcgctttg agttcggcgc ggacttcgtc gggggttttg
     9481 ccgcacataa aggcttctgc ttgtgcaaag acgttggcaa gcaggatttc gtggtgtccg
     9541 ggcaggttgc tgcgtttttc aagcgaggca atcaggtcga tgggggtgat gtgcgtgcct
     9601 tgatgcagca gttggaagaa ggcgtgctga ccgttaatgc ccgtttctcc ccagataatc
     9661 ggcgaggttt cgtgtccgac tgctttgccg tccaacgtaa cctgtttgcc gttgctttcc
     9721 atatcgagct gctggatgaa tttgggcagg cggtgcaaat gttggtcgta aggcgcgatg
     9781 acgtggctgc cgccaccgta gtagttgata taccagatgc cgatgagggc gaggatgacg
     9841 ggcaggttgc gctcgagcgg ggtgttgatg aagtgtcggt ccatcaggtg cgcgccgttg
     9901 agcatttcga tgaagttttc ttcgccgaga tacagcataa tcggcaatcc gatggcggac
     9961 cacaggctgt accgaccgcc gacccaatcc caaaattcaa acatattggc gatgtcgatg
    10021 ccgaattcgg cgacggcttt tcgattggtg gaaacggcgg cgaagtgttt ggcaacggct
    10081 tcttcgtcac ccgcatgatt caaaaaccat tcgcgcgcgg ttagcgcgtt ggtcagcgtt
    10141 tcctgcgtgg taaatgtttt ggaggcgatg atgaacagcg tggtttccgg gtggactttg
    10201 gacaatacgt cgcgcagttg cgagccgtcc acgttggaga cgaagtgcat attgaggcgc
    10261 ggatgaccga aaggtttgag cgcggtacac atcgtcagcg ggcccaaatc cgatccgccg
    10321 atgccgatgt tgacaacgtc ggtaatgact tggttggtat agcccagcca gcttccgctg
    10381 cggacttcgt gtgcaaattc gcccatacgt tgcaaaacgc ggttgacttt gggcatcaca
    10441 tcttcaccgt caaccataat cggcgaattg gtgcggttgc gaagggcgac atgcaggacg
    10501 gcgcggtttt cggtggtatt gattttttcg ccgtggaaca tctgccgcat ccgctccggc
    10561 acgcctgctt cacgcgccaa ctcgaacagt agggacatgg tttcgtcgtt gatgcggttt
    10621 ttggagtagt ccagcgtcag tccgccgact tgcagccagt agcgttccgc ccgctgcggg
    10681 tcttgctcga acatttcgcg catatgcaat gttttgctgt cgtcaaagtg gttccacaat
    10741 tttgaccagg ccggtaagtc gtgaaggtgt ttcatctgta tgctcccgat tgaggttttc
    10801 cgtgacgggg ggcgtttccg cacatcgatg cgcacataag gcgcaaccct gcgtaccgcc
    10861 ccgatttgga aggggttgcg gcaaacccga accctgccgc cttaaagggc ggggtttccg
    10921 ccattaagga aattctgata aaaggcttgc cggagcctgt ccggccgttc agacggcatt
    10981 ggcgggatgc cgtctgaaca tcagtctttg tcgtaatcga tgtgcttgtt gtgtatgctt
    11041 tttttgcttt tctgcaattg caggctggca gcatcgccca agcgcagggc aagtccgatg
    11101 gcgagaatat caatgacggc aagctgcaag aggcgggaaa ccatgggcgt gtagagttcg
    11161 gcgttttcct gtgtggcaac gctcaacacg cagtcggcaa gttgcgccag aggcgaatcg
    11221 ttgcgggtca gtgcgatgac ggacgcgccg ttttctttgg cgatgctgac cgcatccaaa
    11281 agttcgatag acgaacccgt gttggaaatg gcaaccaaaa catcctgatc gctcaaaacg
    11341 gatgccgcca tcagttgcgt gtgcgtatcg acataggcga cggtggacat gccgaaacgg
    11401 aaaaatttgt gctgcgcgtc ctgtgccaca atgccggaat tgcccacgcc gtaaaattcg
    11461 acgcggcggg cgtgcatcag ggtggcgatg ccgttttcga gttcggattc ttttaagaac
    11521 ctcctctccc ccaacaaggc tgcggcggcg ttgcccaaca ctttttcaac cacattcgcc
    11581 atatcgtcgt cggcattgag ttcttcgtgg acataaggca taccttcgtg cccgatgctg
    11641 gcggacaggg agagcttgaa ttccggcagc cccttataac cgaggctgcg gcagaaccgg
    11701 atgacggtcg gctggctgac cgaggcgcgg tcggcgattt cggcgacggc ggcgtggaca
    11761 aaccacttgg gttctgccaa ggcgcattcg gcaactttgc gttccgcacc ggacaggtcg
    11821 gccagtgatt cgctgatttt gcttaacata atgatatgcc cttcgataat gcggccctgc
    11881 tgcaagggct gccgtggtta gacgtttttc aggtggttgc cgagcgccgc cgccgcgccg
    11941 gcaatacccg gaaattcgct caagacgaca taaacgggaa tcgcggcaag gtaggcttca
    12001 aaacgccctt tgttttcaaa gcggctgcgg aagggggagg ttttgaagta atccaacatt
    12061 cgcgggataa tgccgccgca caaatacacg cccccgcgcg cgcccaaagt cagggcgagg
    12121 ttggaagcga ccgtgcccag catcgcgcaa aaaatgtcca atgcctgccg gcacaggggc
    12181 gattcgcaat ttaatgcctt ttcggtgatt tcagacggca tcagttttgc cggttcggct
    12241 ttctgcttgg ccgccagcgt ttcataaatc aggctcaaac ctgcgccgct gaggaaacgt
    12301 tcggcggaaa cgtggcggta tttgtttttg gcgtactgcc aaatcaacac ttccatatcg
    12361 tcaaacggcg ggaaactggt atgcccgccc tcgcccgcca acgccaccca gcctgcgggg
    12421 ctgtgcacca atccgctcac gcccaagccg gtaccggggc cgataacggc cttgggggcg
    12481 aattcgacag gcttttgccc gcctacctgc atcaggtctt tgcttgaagt ctgcgttacc
    12541 gccaatgcct gcgcggtaaa gtcgttcaaa aggatgaggg tgtccagccc caaagcctga
    12601 cgggtggttt cgatggaaaa cgcccaatgg tggttggtca tctgcaccca gtcgcccaaa
    12661 atcgggttgg cgatggcaaa tgccgcgtgc cgtacgcccg ttgcaccact ttggttcaga
    12721 taggcacgca ccgcgtcggt aaccgtgtcg tattccttac acggcaggac ggcgactttc
    12781 tcaatcacgc acggcgcggt ttccaatgcg aaacgggcgt ttgtgccgcc gatgtcggca
    12841 accagcctcg ggtaatcggc gtgtttattc ggcgtagaag acatggcagt tcactccttg
    12901 atggttcaaa acgaggctga tcggatattc gcggttttcg ccttgtgcgg cttggtcgaa
    12961 cacggctttt ttctcttcgc cctgtatcgc caaaaataca tgcccagtat gggcaatcgc
    13021 atccaaagtc atgctgatgc gctcgtgcgg cgcggtaacg ggcgtggtat gcaccagcgc
    13081 gacacctgcc gaaccgtcga ttgccgtctg aaactgcgga gcttttggga aaatcgaagc
    13141 cgtatgcccg tcgtttccca tacccaaaat caaaacatcg ggctgcttgt aatgtttcag
    13201 cgcataatcg acaacagcat cgggatgtaa ttcggtttca gtttttccgt cttccaccat
    13261 aggaatccac actgccgcag cggctttgtt ctttaacagg tattcgcgca ccagaccggt
    13321 attgctgtcg gcgtggttgg tcggcacgat gcgttcatct gccaaggtaa ttccgacgtt
    13381 tttccaatcc aaatcttttt gcgacagggc gttgaaaaat gcaatcggcg aacgtccgcc
    13441 ggaaactgcc aacaccgcac cgcccttctc gtccagtgcg ccctgcaaag catccgccac
    13501 tgcgtcagcc aaagactgcg ccgcttctgc cgcattttcg tattcgtgcc aaacaaacat
    13561 atttgtgtcc ttttgtgatt tcagacggca tatcccgtta cagggcggct gccgggcaat
    13621 ggcttagccg cttccgcctc gaagcgggac tgctttttct gcccgccctc cttcagacgg
    13681 cattgcgaac ccgatgccgt ctgaagcgca ttactgttct tcgtgccact tgttgccgtc
    13741 gcgcgccaac agttcgcggg cggcttcagg gccccaagag tgtgcgccgt aaccgtgcgg
    13801 cggcgtggtg ttatttgccc agttttccaa aatcggcatc acatattccc acgcggcttc
    13861 gagttcgtcg cggcggttga acaaagcgag tttgccgtta atcacatcca acagcaggcg
    13921 ttcgtaagcc tcggcgcggc ggccttcaac ggccttgccc aaatccacgc ccaaaggcgt
    13981 tacttccact ttgttgcccg cacccggggt tttcacctgc gtgtaaaggc ggacggattc
    14041 attcggttga agctcgataa ccaaacggtt cggcgcggtt tggctgttgt cgaaaatatg
    14101 gttttgcagc gggcggaagt tcaaaacgat ttccgccact ttgcccgcca tgcgtttgcc
    14161 ggtacgcagg tagaagggaa cgcccttcca gcgttcgttt tcgatttcgg ctttaatggc
    14221 gacgtaggtt tcggtaaagc tgtcttgcgg aacgttgatt tcttcaagat agccgttcat
    14281 gcctttggca gcggtatatt gtccgcgcac gacattttca ttgacagatt cgatggtcag
    14341 cggcttcaat gacttgatga ctttgacttt ttcatcgcgc accgcgtcgg catccaagct
    14401 ggcgggggct tccatcgcag tcatgcacag catctgcatc aaatggtttt gcaccatatc
    14461 gcgcaacgcg ccggtaatgt cgtaaaactc gccgcgctct tccacaccga gctgttcggc
    14521 gatggtcagc tgcacgcttt cgatgtattt attgttccac agcggctcga acattacatt
    14581 ggcaaaacgc agcgcaagca ggttttgcag gctttctttg ccaaggtagt ggtcgatgcg
    14641 gtaaatttgc ccttctttga aataacgcgc cacatcggta ttgatttgtt gggaagaagc
    14701 caaatccgta cccagcggtt tttccaaaac cacgcgcaca ttgtcggcat tcaaaccgat
    14761 cgcagcaagg ttttcgcagg cttgcgcgaa gaatttcggc gcggtggaca gatagatgac
    14821 gacgttgtcg gtttctttgc gtgctttgac caaatcgccc aaagcggcaa aatcgtccgg
    14881 ctgcgtaaca tcgactttga gatatgcgag acgttcaaca aacgaagccc acgcctcacc
    14941 ggaaaaattt tctttcacat ggattttgga actggtttcc accttcgcca gaaaaccttc
    15001 ggtatccaac tcgctgcggc tgacccccaa aatacgccct tcgggatgaa gcagaccggc
    15061 aacatgcgcc tgatacaggc agggcaaaag tttgcgcatt gccaaatcgc cggtcgcacc
    15121 gaacaacacc aaatcaaaat ttgtctgtgt actcatcgca ttatctcgtc aggaaaggat
    15181 tttttcgatg ccgtctgaaa cctgtttttt ccatcacgca gcatcgcaat atcggaaaca
    15241 aaggcaggcg gcataatgag tagtaatact acacaccgct acactttttg tctattccca
    15301 tttttacaat ttatttgacc tagtccaaaa atcaggcagg tttcccctat tccgttacaa
    15361 caatcagaag attctgcgat ttaaatcaaa tttcttttca atgtctgatt tttttgtaac
    15421 aaaattacaa attttgtact ataatagcac ccgcttccca ctttcagacg gcgtaccttt
    15481 taaaatatag tgaattaaat ttaaatcagg acaaggcggc gagccgcaga cagcacaaat
    15541 agtacggcaa ggcgaggcaa cgccggaccg gtttaaattt aatccactat actcaccgtc
    15601 taacataccc gatacaaaaa tcagaaacgc acaaacaaat ccccaatacc accccgttcc
    15661 gccaggagac cgaacatgaa ccacactcct atccacccca aactcgccga aatcaccggg
    15721 cgcattatcg aacgcagccg tccgacgcgt gaaaaatatc tggcgaaaat ccgcagtgcc
    15781 aaacaaatgg gacgcttaga gcgcaaccag ctcggctgca gcaatttggc gcacggttat
    15841 gccgccatgc ccaaaagcat caaaatcgaa atgcttcagg aaaccgtccc caacttaggc
    15901 atcatcactg cctacaacga catggtttcc gcacaccagc cgtttaaaga cttccctgac
    15961 caaatcaaag acgaagcaca aaaaaacggc gcgaccgcac aagtcgccgg cggcacgcct
    16021 gccatgtgcg acggcatcac gcaaggctac gccggcatgg aattgtcgct gttttcccgc
    16081 gacgtgattg cgatgagtac cgccgtcggg ctgtcgcacc aaatgtttga cggcagcctg
    16141 tttatgggcg tgtgcgacaa aatcgttccg ggtctgatga taggtgcgct ttcgttcggt
    16201 catattcccg gtatcttcgt tccggcaggc ccgatgtcca gcggcatcgg caataaagaa
    16261 aaagcccgta cccgccaact tttcgccgaa ggcaaagtcg gacgcgacga acttttgaaa
    16321 agcgaaatgg gttcttacca cagcccgggc acctgcactt tctacggcac ggcaaactcc
    16381 aaccaaatga tgatggaaat gatgggcgta cacctgcctg ccgccgcctt tgtccacccc
    16441 tataccgacc tgcgcgaagc cctgacccgc tacgccgccg gacacctcgc gcgcggcatc
    16501 aaaaacggca cgattaaacc tttgggcgaa atgttgaccg aaaaatcctt catcaatgcc
    16561 ctgatcggtt tgatggcgac cggcggctcg accaaccaca ccatgcacct cgtcgctatg
    16621 gcgcgcgccg caggcgtgat tttgaactgg gacgacttcg acgaaatttc ttccatcatc
    16681 ccgctgctca tccgcgtgta tcccaacggc aaagccgacg tgaaccactt taccgcagca
    16741 ggcggcctgc ctttcgttat ccgcgaattg ctggacgcag gcttattgca cgacgatgtc
    16801 gataccgtcg tcggacacgg tatgcgccac tacactaaag agcctttcct catcgacggc
    16861 aaactcgaat ggcgcgaagc ccccgagacc agcggcaacg acgacatcct gcgtaaagcc
    16921 gacaatccgt tctccccgga cggcggtctg cgcctgatga aaggcaacat cggacgcggc
    16981 gtggttaaag tgtcagccgt acgcgaaggc tgccgcatca tcgaagcacc cgccatcgtg
    17041 ttcaacgacc agcgcgaagt gttggcggca tttgaacgcg gcgagttgga acgcgatttt
    17101 atctgcgtcg tccgctacca aggcccgcgt gccaacggta tgcccgaatt gcacaaactg
    17161 accccgcctt tgggcatcct gcaagaccgc ggcttcaaag tggcgctgct gaccgacggc
    17221 cgtatgtccg gcgcgtcagg caaagtgccc gcgtccatcc acatgacgcc cgaagccctg
    17281 atgggcggca acatcgccaa aatccgtacc ggcgacctga ttcgcttcga ctccgttacc
    17341 ggcgaactca acgtcctgat taacgaggcc gaatggaacg tccgagaagt cgagcgcatc
    17401 gatttgggcg cgaaccaaca aggctgcggc cgcgaactct tcgccggctt ccgcagtatg
    17461 accagcagtg cggaaaccgg tgcgatgagc ttcggcggag aatttgcctg atgcgcgttt
    17521 cagacggcct tttaaaacag aaggccgtct gaaaaattat tgaagccgtt taaaatagat
    17581 gcagacgtag cgtgagcttt gcccgcgaac aaaacccaag caacatcaga atgttttacc
    17641 ccaaaggttg tctgaaaact taaccccccc catcccgttt gacctcaaca aaaacgacac
    17701 cccccacttg gagaaccgaa atgtccaaac tgaccccccg ccaaatcctg accgccggcg
    17761 cagttgtgcc tgtgatggcg attgacgact taagcaccgc cgtcgatttg tcccgcgccc
    17821 ttgtcgaagg cggcatcccc accctcgaaa ttaccttgcg cacccccgtc ggactcgagg
    17881 ccatccgcct gattgccaaa gaaatgccca acgccatcat cggcgcaggt acggtaacca
    17941 atcccgaaca gctcaaagcc gtcgaagacg caggcgcggt tttcgccatc agcccgggtt
    18001 tgcacgaatc cctcgccaga gccggccgca acagcggcat tcccctgatt cccggcgttg
    18061 ccactccggg cgaagtccaa ctggctttgg aacacggcat cgacacactc aaactcttcc
    18121 ctgccgaagt cgttggcggc aaagcgatgc tcaaagccct ctacggacct tacgccgacg
    18181 tacgcttctg cccgaccggc ggcatcagcc tcgccactgc gcccgattac ttggcattgc
    18241 ccaacgtctt atgcgtcggc ggctcttggc tgacaccgaa agaagctgta aaaaacaaag
    18301 actgggacac cattacccgc ctcgccaaag aagcggcggc attgaaaccc aaagcctgaa
    18361 ccgcatcgta aaaatgccgt ctgaagccct ttccagtctc agacggcatt ttgccgcttg
    18421 gcgactcagt cggcatacac agcagcaccg cccctaaaat gcccatccat ctccagaaca
    18481 atgcgaatga cgcgcgatat
//
LOCUS       DAWTHD010000031        18419 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWTHD010000031 DAWTHD010000000
VERSION     DAWTHD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.031"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            38..532
                     /locus_tag="WM704_000845"
     CDS             38..532
                     /locus_tag="WM704_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340297.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
     gene            652..1785
                     /locus_tag="WM704_000846"
     CDS             652..1785
                     /locus_tag="WM704_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340298.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            complement(2288..2563)
                     /locus_tag="WM704_000847"
     CDS             complement(2288..2563)
                     /locus_tag="WM704_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9340299.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMPSET"
     gene            complement(2560..3843)
                     /locus_tag="WM704_000848"
     CDS             complement(2560..3843)
                     /locus_tag="WM704_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9340300.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(3992..5410)
                     /gene="glnA"
                     /locus_tag="WM704_000849"
     CDS             complement(3992..5410)
                     /gene="glnA"
                     /locus_tag="WM704_000849"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFA9340301.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(5411..5626)
                     /locus_tag="WM704_000850"
                     /pseudo
     CDS             complement(5411..5626)
                     /locus_tag="WM704_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
     gene            5721..6530
                     /gene="aroE"
                     /locus_tag="WM704_000851"
     CDS             5721..6530
                     /gene="aroE"
                     /locus_tag="WM704_000851"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFA9340302.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            6533..7234
                     /gene="mtgA"
                     /locus_tag="WM704_000852"
     CDS             6533..7234
                     /gene="mtgA"
                     /locus_tag="WM704_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFA9340303.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(7508..7583)
                     /locus_tag="WM704_000853"
     tRNA            complement(7508..7583)
                     /locus_tag="WM704_000853"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7548..7550),aa:Phe,seq:gaa)
     gene            complement(7774..8508)
                     /gene="lptB"
                     /locus_tag="WM704_000854"
     CDS             complement(7774..8508)
                     /gene="lptB"
                     /locus_tag="WM704_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFA9340304.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(8579..9109)
                     /gene="lptA"
                     /locus_tag="WM704_000855"
     CDS             complement(8579..9109)
                     /gene="lptA"
                     /locus_tag="WM704_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFA9340305.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(9090..9671)
                     /gene="lptC"
                     /locus_tag="WM704_000856"
     CDS             complement(9090..9671)
                     /gene="lptC"
                     /locus_tag="WM704_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFA9340306.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(9668..10204)
                     /locus_tag="WM704_000857"
     CDS             complement(9668..10204)
                     /locus_tag="WM704_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9340307.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(10270..11244)
                     /locus_tag="WM704_000858"
     CDS             complement(10270..11244)
                     /locus_tag="WM704_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFA9340308.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            11341..12396
                     /gene="tal"
                     /locus_tag="WM704_000859"
     CDS             11341..12396
                     /gene="tal"
                     /locus_tag="WM704_000859"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFA9340309.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            12547..12837
                     /locus_tag="WM704_000860"
     CDS             12547..12837
                     /locus_tag="WM704_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340310.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            12854..13741
                     /gene="gluQRS"
                     /locus_tag="WM704_000861"
     CDS             12854..13741
                     /gene="gluQRS"
                     /locus_tag="WM704_000861"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFA9340311.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(13811..14827)
                     /gene="dusA"
                     /locus_tag="WM704_000862"
     CDS             complement(13811..14827)
                     /gene="dusA"
                     /locus_tag="WM704_000862"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFA9340312.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            14947..16101
                     /locus_tag="WM704_000863"
     CDS             14947..16101
                     /locus_tag="WM704_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFA9340313.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(16151..16417)
                     /locus_tag="WM704_000864"
     CDS             complement(16151..16417)
                     /locus_tag="WM704_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFA9340314.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(16525..17403)
                     /locus_tag="WM704_000865"
     CDS             complement(16525..17403)
                     /locus_tag="WM704_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9340315.1"
                     /translation="MRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
     gene            complement(17444..17674)
                     /locus_tag="WM704_000866"
     CDS             complement(17444..17674)
                     /locus_tag="WM704_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340316.1"
                     /translation="MRTHIRTCVYHDSGTKGFKHGIRHKRHGCRRGGTSVFQRGEDGK
                     PQRVSRIRKRSATPGQARERTKTHDVSRTQQP"
     gene            complement(17671..18408)
                     /locus_tag="WM704_000867"
     CDS             complement(17671..18408)
                     /locus_tag="WM704_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340317.1"
                     /translation="MRYACNQELINFAEHIVKQVQRLGLYCNTDDPANEESVLFACRE
                     ASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLAVRV
                     AKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFTGRILHAANLLYDVGIQ
                     ADKELSAMYGRPLNPVRPRRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCDNGA
                     GFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT         4186 a         4926 c         4997 g         4310 t
ORIGIN      
        1 aatttaatcc actataattt ggaagaggca gcaacctatg gaaaatagat ttgaaaaaat
       61 cgttaaggat atttttagtt ttacccaatt tgtttccgaa gcggaaaata aaattcctaa
      121 ccaatcctcc cgttattacg actgctggtg ggaattggaa atcctgaatg cctccgcatt
      181 ggaagattgg gaaatatcgg gaaagcctga aaaatgggag aaatggacag aaaaatatca
      241 gaccgaagcg ttacagtcag ttaaggatct atttgccgaa gaagttcctg aaaataatat
      301 agtttgcgat atttctcatt ttttgcattt tgccgatcgg gcagaagccg acattcccaa
      361 tcaatcacgt ttatatgccg aatattggtg ggaaatggaa acgttcaaaa tgtcggtttt
      421 aaaagattgg gagcgttcag gcagaccact agattgggaa aaatggcaaa ggcaatatca
      481 atccgaagca tttgaattag tcaaaaattt gaaccgaagc aaacaagagt aacaagccgc
      541 agcctgcata ggggttttta aagtacggca gcaaccgtag attagattaa aaatcaaaca
      601 atcaaagccc gcctgatata atcgatatat cctgttaaac gaaaaataaa tatgtttaaa
      661 aaattcaaac cggtattgtt gtcatttttt gcacttgtat ttgccttttg gctgggaacg
      721 ggcattgcct atgagattaa tccgcgttgg tttttgagcg atacggcaac tgaaatacct
      781 gaaaatccga atacttttgt ggcgaaactt gcccgcctgt tccgaaatgc cgacagggcg
      841 gttgtcatcg tgaaggaatc gatgaggacg gaggaaagcc ttgccggagc tgtggatgac
      901 ggtccgttgc agtcggagaa ggattatctc gcgctcgctg tccggctcag tcgtttgaaa
      961 gaaaaggcga aatggtttca cgtaacggag caggaacatg gggaagaggt ctggctggat
     1021 tactatatcg gcgagggcgg tttggttgcg gtttcgcttt tgcaacgctc gccggaagcg
     1081 tttgttaatg ccgaatatct gtatcggaac gatcgtccgt tttctgtaaa tgtgtacggc
     1141 ggaacggctc acggggaaaa ttatgaaacg acaggagaat atcgggttgt ttggcaaccg
     1201 gacggttcgg tatttgatgc gtcggggcgc gggaaaatcg gggaagatgt ttatgagcat
     1261 tgcctcgggt gttatcagat ggcccaggta tatttggcga aataccggga tgtcgcgaat
     1321 gatgagcaga aggtttggga cttccgcgaa gagagcaacc ggattgcatc ggactcgcgc
     1381 gattctgtgt tttatcagaa tatgcgggaa ttgatgcccc gggggatgaa ggcgaacagt
     1441 cttgtggtcg gctatgatgc ggacggtctg ccgcaaaaag tctattggag tttcgacaat
     1501 ggaaaaaaac gccagagttt cgaatattat ttgaaaaacg gaaatctttt tattgcacaa
     1561 tcttcgacgg tagcattgaa agcggatggc gtaacggcgg atatgcagac ctatcatgcg
     1621 caacagacgt ggtatttgga tggcgggcgg attatccgcg aagagaaaca gggagacaga
     1681 ctgcctgatt ttcctttgaa cttggaagat ttggaaaaag aggtgagccg ttatgcagag
     1741 gctgcggcga gacgttcggg cggcaggcgc ggcctttctc actgaacgca aataccgtct
     1801 gaaaccggtt ttcagacggc attggagagt caaacgcttt ttgcaatgcc gtatatgccg
     1861 aacccggttc gctacaaatt gaaaaccaca atccaaaaaa cggaaataca cattatcaaa
     1921 caaacaaata agttgacata ttgtgcgata ttgagggctg taccgtattt tcccgttctt
     1981 tatatgagcg gcaggcatcc tcataaaaga agggataccg ataaggaaaa atccctcggt
     2041 ataaaaaata tactgattaa tcaatacgaa tccaacaaac agttcatcat tttcttttct
     2101 gttggcgtat aaaaaccgct cggaaaaaaa taaaatatca aaacggcaaa ataaaaatac
     2161 tgatatatgc cttcactttc atagggcgac gctctatgtg ccgtcctgtg tgttgaaacg
     2221 tcggttttcc ttaccgcctg ccccgctttt ccctgattgc tgccaactgc cgtccgaacc
     2281 tgaaccgtca ggtttcagac ggcatttttt ctccctgttc ccacaattgc cgtttttgtg
     2341 cggcggtcag ttttttgacg gcgatttcct gtttgagcgc agtgccttta tccatgccgc
     2401 ctgcaacgat acgcatcgcc accggtttga atacgcgggt atatttcgcg ccttttccgg
     2461 ctgtgtggat ggcaagccgc tgttgcggat tcgggctgat accgcaatag aacgcgctgt
     2521 tttcacacaa tatcaggtaa acgctccagt ttgacgcgtt catctgcctg catcctgggt
     2581 tttttccccg ttccaaggcg caactttcag cagcagcagc atacccggca gggcaagtat
     2641 gaaacacagc cggaaaaacg gtacataacc catccattcg atcagatagc ctgcaaagga
     2701 attgatgacc gtgcgcggca cggcggacag gctggtaaac agcgcaagct gcgttgccgt
     2761 aaacgcggga ttggtctcgc gcgccatata cgatacaaac gccgccgtcc ccaaccccac
     2821 gccgaccgct tccgcgccga taactgccgc cagcatcagc ctctcgcctg tgccgaccgt
     2881 gtcgaaatgt ccgaaccctg ccagccatac aaaccccaaa accgttattg cctgcaccgc
     2941 gccgaatagc cacaaggctt tgtttacgcc gattttcagc atccacacgc cgcccaagat
     3001 acctgccgcc actgccggcc acagtcctgc atttttggca atcaaaccga tgtcggtctt
     3061 gctgaaaccc atatccagat aaaacggcgt tgccaacgcg gttgccatac tgtcgccgag
     3121 tttgtaaagg aagataaaca gcagcacgca caccgccgaa gcgatgccct tgcgcgtaaa
     3181 aaattcttta aacggctcta ccacggtctg cttcaacgtt ttaggaacgg aaggcggcaa
     3241 cacgggttcg tgcgcgagaa acagcgtcat cagaaggccg ggcagcataa ataatgaagt
     3301 gataacaaat acttctgacc acggcatcct gtctgccaac accaaactca atgaaccggg
     3361 aatcagggcg gcaacccggt aggcgttcac atgaaccgag ttgcccaaac ccaattcttc
     3421 gtccgacaaa atctcgcgcc tgaacgcatc caatacaatg tcctgactgg cggaaaaaaa
     3481 agcgacaagc accgacaggc cggcaatcaa cggcagatga ttccgggggt ttaaaaaggc
     3541 atatgccgcc aaagccgcca gtaaccctgc ctgcgtcagc agcatccacc cgcgccgccg
     3601 tcccaaaacg ggcagcctga ccgcgtccat cagcggcgac cacaaaaatt tccaagtaaa
     3661 cggcagaccg atcaacgcca tcagcccgat gctcttcaaa tccacctgct cgctgcgcaa
     3721 ccacgccgga atcaggttaa tcagaaagta cagcggcagc cccgaggcaa accccgtaaa
     3781 aatacagacg agcatattac gggaaaagat ttgcccgata aaacctgatt tcgatgcagt
     3841 catcgttcgt acagccaaaa aacaaaagcc tattgtagca aatatcgccc gcctgctgaa
     3901 aaatgccgcc acgcgccgcc ggtttgccgt tccaatgcaa aatgccgtct gaaactgtgt
     3961 tcagacggca ttgctactga aacccgacgg tttacaggct gtaatacatt tcaaattcca
     4021 gcgggtgcgg cgccatacgg atgcggcgga catcttcctc tttaaaggcg atgtagctgt
     4081 cgatccagtc tttgctgaac acgccgccgc gcagcaggaa ttcgtggtcg gccttgaggg
     4141 cggcaagtgc ttcttccaaa gaagcgcaga cggtcgggac gagcgcgtct tcttccggcg
     4201 gcaggtcgta caggttttta tcggcagggt cgcccggatg gattttgttt tgaatgccgt
     4261 ccaaaccggc catcagcagg gcggcaaatg ccaaatacgg gttggcggtc gggtcgggga
     4321 aacgcgcttc gatgcggcgc gccttgctgc tgttcacaga cgggatacgg atggaagcgg
     4381 aacggttttt ggcggaatag gccaatttgg tcggtgcttc aaagtgcggc acgaggcgtt
     4441 tgtaggagtt ggtggacgga ttggtaatcg cgttcagggc tttggcgtgt ttgatgatgc
     4501 cgccgatgta gtagagcgcg gtatcggaca aaccggcata gccgtcgcct gcgaacaggt
     4561 tttgaccgtc tttccaaatg gattggtgga cgtgcatacc gctgccgttg tcgcccataa
     4621 tcggtttggg cataaaggtg gcggttttgc cgaaattgtg ggcaacgttt tggatgacgt
     4681 atttcatatc ttgggtttgg tcggcgcgtt tgaccaaagt ggcgaaacgg gtgccgattt
     4741 ccatttggct gccggtaccg acttcgctgt ggtggacttc gacttcgatg ccgagtcctt
     4801 ccaaaatgtt caccatggcg gagcgcaaat cttgaccgca gtcaatcggc gcgacgggcg
     4861 cgtagccgcc tttgacggcg gggcggtggc cggtgttttg accgtccata tgcaggccgc
     4921 ttgcccacgc gccgctttcg gacgtgattt cgtaacgggt tttgtgcatg tcggtttcaa
     4981 attctacgcc gtcgaagacg aagaattcgg gttcggggcc gaaataggcg gtgtcgccga
     5041 taccggaaga tttcaaatag gcttcggcgc ggcgtgcgat ggagcgcggg tcgcggtcgt
     5101 aaccctgacc gtcggcaggg tcgatgacgt cgcaggtaat gacgacggta acatcatcat
     5161 aaaaaggatc gacgaaggct gtggacgcat cgggacgcag ctgcatatcg gaagcctcaa
     5221 tgcctttcca gccgccgatg gacgagccgt caaacgccgg tccgttttca aaccactctt
     5281 cggggtcttc caacacgatg cgcgcaggca cggtaaagtg gtgctgcttg cctttggtat
     5341 cggtgaagcg caaatcgaca aagcgggctt cgctttcttc aatcaatttt acggcgtttt
     5401 taatggacat cttcagctcc tgaaaaaggg aaatggcaaa atatgcggaa cgcggcggct
     5461 tgacgcaaag tcggcacgat tttaccacgc cgatgccgtc tgaaaccggt tttgcgtcaa
     5521 attccaagcc ggaaaatata gtttcaaacg gcaagattgt caacaatatg cttcggtttg
     5581 tctgttcaaa agcggaatgc ccgccctttt gtccgaatgc cgacaaaaca gatgccgtct
     5641 gaaggcggtt tcagacggca tcgttcccat cttcgcgccg ccgttataaa atgaccgccc
     5701 cgcctttatg gaatatgact atgcacgccc tcccccgcta cgccgttttc ggcaaccccg
     5761 ccgcccacag caaatcgccg cagattcatc agcaatttgc ccttcaggaa ggcgttgaca
     5821 tcgaatacgg acgcatttgc gccgacatcg gcggtttcgc gcaggcggtt tcgacatttt
     5881 ttgaaacagg cggttgcggg gcaaacgtta ccgtaccgtt caaacaggaa gcgtttcatc
     5941 tggcggacga acattccgac cgcgctttgg ctgccggtgc ggtcaatacg ctggtgtggt
     6001 tggaagacgg cagaatacgc ggcgacaata ccgacggtat cggtttggcc aacgacatca
     6061 cgcaggtcaa aaacattgcc atcgagggca aaaccatctt gcttttgggc gcgggcggcg
     6121 cggtgcgcgg cgtgattccg gttttgaaag aacaccgccc tgcccgtatc gtcattgcca
     6181 accgtacccg cgccaaagcc gaggaattgg cgcggctttt cggcattgaa gccgtcccga
     6241 tggcggatgt gaacggcggt ttcgatatca tcatcaacgg cacgtccggc ggcttgagcg
     6301 gtcagcttcc tgccgtcagc cctaaaattt tccgcgactg ccgccttgcc tacgatatgg
     6361 tttacggcga agcggcaaaa ccgtttttag attttgcacg acaatcgggg gcgaaaaaaa
     6421 ctgccgacgg actgggtatg ctagtcggtc aggcggcggc ttcctacgcc ctctggcgcg
     6481 gtttcaaacc ggacatccgc cccgtcatcg aacacatgaa agccctgtaa ccatgttccg
     6541 catcgtcaaa tggctgattg ccctgcccgt cggcatcttt atcttcttca atgcctatgt
     6601 gtacggcaac atcattacct accgcgccgt cgcgccccat cggactgcct ttatgtcgat
     6661 gcggatgaag cagtttgaac aagaaggtcg cgatgtcgca ctggattacc gctgggtgcc
     6721 ctacaaccgc atttccacca acctgaaaaa agccctgatt gcttccgaag atgtccgttt
     6781 tgccggacac ggcggcttcg attggggcgg cattcaaaac gccatcaggc gcaaccggaa
     6841 cagcggcgaa gtgaaggcgg gcggatcgac catcagccag cagcttgcca aaaacctctt
     6901 cctcaacgaa agccgcaact atctgcgcaa aggggaagag gcggccatta cggcaatgat
     6961 ggaagctgtt accgacaaaa acaggatttt cgaactgtat ttaaactcaa tcgaatggca
     7021 ctacggcgtt ttcggcgcgg aagctgcgtc ccggtatttt tataaaaaac cggccgcaga
     7081 cctgaccaaa cagcaggcgg cgaaactgac ggcactcgtc cccgccccgc tctactacgc
     7141 tgaccatccg aaaagcaaac ggctgcgcaa caaaaccaat atcgtgctca gacgcatggg
     7201 ttcggcagaa ttacccgaaa gcgatacgga ctgattgttt cagatatgga aatgccgtct
     7261 gaactgcggt tcggacggca tatgttttct ggttcttata gtggattaaa tttaaaccgg
     7321 tacggcgtta ccccgccttg ccgtactatg ttgtactgtc tgcggcttcg tcgctttgtc
     7381 ctgatttttg ttaatccact aacctgcccg acagccgcaa agaccgtatc gtgcatggca
     7441 ggtatttgcg gtgcgggact gctgccgaaa acggcaggca aaaaataacc gcttataagc
     7501 ggttttttgg tggccagagg cggaatcgaa ccgccgacac acggattttc aatccgttgc
     7561 tctaccaact gagctatctg gcctgtatct gtctcgttaa gaggtgcgta ttaaaccaaa
     7621 tttcgggacg acacgcaagc ggatttgtcg atatttttta cccaaccgac taagtcgatg
     7681 ttttaagttt aaattaattt cacagaaaaa tagtgcaaat cggttttttg ccgttcgttc
     7741 ggcgatgttc tgctttgcag acggcaaaac gcttcaatat ttgaagttct cgcccaaata
     7801 gacggcgcgc acctgttcgt tgccgaccaa atcgtcgggc ttgccggaag ccagcaccgc
     7861 accgtctgaa atgatgtagg cgcggtcgca gatgctgagg gtctcgcgga cgttgtggtc
     7921 ggtaatcaat acgccgatgc cgcgcgattt gaggaaaccg atgattttct ggatgtcgat
     7981 gacggcgatg ggatcgacgc cggcaaaagg ttcgtccaaa aggatgaaac gtggtttcat
     8041 ggcgaggaca cgggcgattt cgacgcgccg ccgttcgccg ccggacagcg acggcgcagg
     8101 attgcggcgc aagtgtccga tattgaggtc ggcgagcagt ttttcgattt ctctgtcgat
     8161 ttgattttta tctttggttc tgatttccaa gatggcgcgg atgttttgtt cgacggtcat
     8221 tttgcggaat atcgaggctt cctgcggcag gtagccgaca cccaggcggg cgcgttcgtg
     8281 tatgggcagg tggcgcaatt cccgcccgtc cagcatcacg ctgcctgcgt cggcagcgat
     8341 gagcccgact atcatgtaga agccggtggt tttgcctgcg ccgttgggcc cgagcagtcc
     8401 gatgacttcg ccgctttcga tctcaaggga gaaacttttg acgacttggc gttttttgaa
     8461 acttttctgc aggttttgga cgacgaggcg gctgttgttt gcgctcataa atctcttctc
     8521 ggcatttcgg tcggcaaatg ccgtctgaac tgcgtttccg tttcagacgg catcggggtt
     8581 attcggtttt ttgtgtgctt gaaggctgga tgacgacgct gaccctgccg gttttggaag
     8641 cggatttcgc acccgatttc gtgctgccgt tgatggtata gacttcggtt ttggtgttgt
     8701 aggtaatgac cgcaccttcg gcaacgtcgc cgccgcgctg cactttggca ttgccggtca
     8761 gaacgacggt gcttcctgcg gaggaatagg taacgttgtt tgcctgaccg cgcaccgtcc
     8821 ctttgccccc gtccaacgtt tggctgaagc ggacgggcga accttccgcc ctcacggatt
     8881 cgccgccttt gccgccgcgt gtgacgttga cgcgcgaggc ggaaatgttg agcgtaccct
     8941 gtctgatgat gacattgccg ctaaatgtgg tactttggtt ggcttgatcg agcgaacctt
     9001 ggtcggcttc gatttggatg ggccgtctgc tgtcgctttg aagggcaaaa gcgggagaag
     9061 ttgcaaaaat tacaattaaa acaaatagct tacatatctt ttgtatcata aatcgcggct
     9121 ttcactttag atgagaagtt caacatgcct gttttgtggt tgtaggtcat accgcccgcc
     9181 tgaccgtgcg acgcgccata ttggaaactg acaggcgtat cggtttgggc atattgagat
     9241 tcggtatcga cgtgcagttt ttcggtttcg actttacccg cctgccgcct gccgtcggcg
     9301 gttttggtca gcacaacgtt gtttttaaaa agaacctgtt tgttttcggt atggtaaacg
     9361 gcttcatcgc tgccgacttc gtacaacagc ctgccttctt ggaagaacac gagatgcggc
     9421 gaatcaaaat ggatgtcgct gttttcggga aactgtttcg cacctttcgc gctcaaatgt
     9481 tctttcaagt atccctgttc gtcaaaccgc cttccgtcca agccgtccat tgtgtattga
     9541 ggttcgtcgg gattgagcct gacttcctcg atttcgactt cgctgatacg gcccaaccat
     9601 gccgacaggc tgcccaaggc aaccgccaat atcaatggga acgcaattcc gtaccgccat
     9661 cttactttca tttgatgtac ccgttcaaag ccgcgcccaa cgtacctttc gcgcggataa
     9721 tcaggtcaca cacttcgcgc accgcgcccg caccgccggg gcgtttggca atataggcgg
     9781 cgtgttgcag cgtaaaccaa tgcgcgtcgg ggacggcaac cggcaatccg cagcgcacca
     9841 tcaccggcaa atcgaccacg tcgtcgccga caaaggcgca ctcggcttct tccaagcctg
     9901 cctgcgcgcg cagctcttca tacgcggcgc gtttgtccga aatgcctttg aagtagtaat
     9961 ttatgcccaa ctgtttgacg cggatgccga cggagggcgc gtcccgacct gtgataatcg
    10021 cagtctgcac gccgcttgcc tgaagcattt tcaggccgtg tccgtccagt gtgtgaaacg
    10081 atttgatttc ttcgccgtcg tcgcggataa agatgcgccc gtcggtcaaa acgccgtcca
    10141 catccaatat cagcagtttg attttggcgg cgcgtgcctg taattcggga gaaattgcct
    10201 gcattgggat ttccttgttt tcagacggca tatcttacca caaatgccgt ctgaaacggc
    10261 tgcaacgcct tatacaatcc gcgccgccaa aaggtcgtgc atattcagcg cacctgtcag
    10321 cacgccgtct gcatcggtaa ccagaagccc gttcacatgg tttgcctgca tgactttcag
    10381 ggcttcggtg gcgaggcgtt cggcggagat ggttttaggg tgtgtatgca tgacttcgtc
    10441 tatcgaaaga ccggtaaaat tgtcgcattc ttgaaacagg cggcgcaaat cgccgtcagt
    10501 aaacacgcct ttcagacagc cttgcccgtc cgttaccgcc aacatgccca aacctttctc
    10561 gctcatgctg acgatggctc ctttcaaggg cgtgccgagt cggacggcgg gcaggccgcc
    10621 gcctttgtgc ataatgtcgg caacgcgcaa aagcaggcgt ttgccgaggc tgccggcagg
    10681 gtggatcaag gcgaagtcgt cgggcgtgaa cgcgcgggcg cgcagcagga cgaccgccaa
    10741 cgcgtcgccc aaagccataa cggcggtggt gctggtggtc ggggcaagcc ccaacgggca
    10801 ggcttcttgc gaaaccgatg cggtgatgtg gatatcggca tggcgcgcca tggttgaatc
    10861 ggggcgggcg gtgatgcaga cgagggtaat gtctttgcgt ttgagcgcgg ggatgatggc
    10921 ggtgatttcg tcgctttcgc cggaattgga aatggcggcg acgacgtcgt tgtccacaat
    10981 catgcccaaa tcgccgtgtg ccgcttccgc agggtggacg aaaaacgcgg gcgtgccggt
    11041 cgaggccatg gttgccgcca ttttgcgccc gatatgtccg gacttgccca tacccgtgat
    11101 gacgaccctg cccttgcagt gcaacaacgc gtctgccgca aggacgaagt tttcgtccaa
    11161 ttccgccgca atttcgcgca agccttccgc ttcggtgtgc aacacttcgc gcgcccagtc
    11221 gagatatttt tcgttttctg ccattgccat ccgcctttgc tgtaacaaaa ctacccaaaa
    11281 tttccgaatc ggggtagcat aagccttcaa accccgccca tccatccaaa ggaatcaaaa
    11341 atgactattt tatcggacgt taaagcatta ggacaacaaa tctggctgga caacctttcc
    11401 cgctcgctcg tgcaaagcgg cgaattggcg caaatgctca aacaaggcgt gtgcggcgta
    11461 acttccaatc ccgccatttt ccaaaaagcc ttcgccggcg acgcgcttta tgccgacgag
    11521 gtcgccgccc tcaagcggca aaacctcagc cccaaacaac gctacgaaac catggcggtt
    11581 gccgacgtac gggcagcctg cgacgtttgc cttgccgaac acgaatccac cggcggcaaa
    11641 accggtttcg tcagtctcga agtttctccc gaacttgcca aagacgctca aggcacggta
    11701 gaagaagccc gccgcctcca tgccgccatc gcgcgtaaaa acgccatgat taaagtgcct
    11761 gccaccgacg caggcatcga tgcgctcgaa accctcgttt cagacggcat cagcgtgaac
    11821 ctgaccctgc tgttctcacg cgcccaaacc ctcaaagcct acgccgccta cgcgcgcggc
    11881 attgccaaac gcttggcagc cggacaaagc gttgcccata tccaagttgt cgccagcttc
    11941 ttcatctcgc gcgtggacag tgcgctggat gcaacgctgc ccgaccggct caaaggcaaa
    12001 accgccatcg cccttgccaa agccgcctat caagattggg aacaatattt caccgccccc
    12061 gaatttgccg cactggaagc ccaaggcgca aaccgcgtgc agcttttatg ggcatctacc
    12121 ggcgtgaaaa acccagccta tcccgacacg ctctacgttg acagcctgat cggcgtgcac
    12181 accgtcaaca ccgtccccga tgccacgctc aaagccttta tcgaccacgg cacggcgaaa
    12241 gcgacgctga ccgaaagcgc ggacgaagca cgggcgcggc tcgccgaaat tgccgcgctc
    12301 ggcatcgatg tcgaaacctt ggcggcgcgt ttgcaggaag acggtttgaa acagtttgaa
    12361 gaagcctttg aaaaactgct cgcgcctttg gtttaggcat ttccccgatg ccgtctgaaa
    12421 ggctttcaga cggcatcgcc cttttgaccg gacacagtgc ggcatatcaa ggatccgccc
    12481 taaaaatatt aatgggcaag aagttaatgc acgacattac ccgccaacac aaaaggaaat
    12541 agaatcatgg caaaagcact cgaaatcatt tcacccgatg aaatttattc agatctgatt
    12601 tttaaggatc cggtacctcc ccatactata tatacagaat tgatgaaact ggttggtaga
    12661 aatgcaggca atgagagaat tgcagataga gccttcgatt ttttctcgcc tgctcttatg
    12721 gatgattcag ctaccgaaga gcaatataat gccctatatg atttaacctt gcttgaagag
    12781 cccgggatgg aactggataa agatgagata acggctttga ttaatagtct taaataaagg
    12841 ggctgtaccg gatatgtaca caggacgctt cgcccccagc ccgaccgggt tgctccacat
    12901 cggctctctt ctgaccgccg tcgcttccta tgccgatgca cgttcaaacg gcggcaaatg
    12961 gctggtccgt atggaagacc tcgatccgcc gcgcgaaatg ccgggggcgg caagccatat
    13021 cctgcacacg ctcgaggcat tcggattcga gtgggacggc gaagtcacct atcagagccg
    13081 ccgttacgcc ctgtatgaag aaaccctata ccgtctgaaa accgccggac tggtctatcc
    13141 ctgccattgc aaccgcaaag actggcaggc cggggcaagg cggggcacag acgggttcgt
    13201 ctataacgga cgctgccgcc accccggcca acgccctgca ctgcaaggca aacagccgtc
    13261 gtggcgcatc cgcgtccccg accgcgacat cggcttttca gacggcatcg tcggcagtta
    13321 cacccaaaac ctcgcccgcg acatcggcga tttcgtcctg ttccgtgcag acggttactg
    13381 ggcataccag ctcgccgtcg ttgccgacga tgccgaacag ggcgttaccc acatcgtgcg
    13441 cggacaagac ctgctcgttt ccacaccgcg ccaaatctat ttgcagcagt gtttgggcgt
    13501 tccgacaccg caatatgccc acctgccgct gctgaccaac gcgcaagggc agaaatggtc
    13561 gaagcagacg ctcgcccccg cattggattc aaaccgccgc gaacaacttc tccgccaagt
    13621 gttccgctac ctcaagctgc ccgaagcacc ggaaaccgac cgccctgccg aattgctcga
    13681 ctgggcggtg gcacactggg atatggacaa agtgccgaaa cacgccatta ccgcccccta
    13741 aacaccaccc gacaaacgca caaatgccgt ctgaaacctt cagacggcat ttcacacata
    13801 cagccccgcc ctattcgcgc gtatttgccc gttcgacttc tttccacgct tcgaaaatca
    13861 ggccgccgtc gttgtctttc aagagcgttg catcggaaag catacgccgc caagtccgcg
    13921 cgcctttcaa accgtgcatc agcccgaggc tgtggcggac gatgtggcgc aggattgtgc
    13981 cgcgtccggc ttggatttgg acttggctgt atgtgtagag gcgctgcacc aaatcggcgt
    14041 attcgatcgg ggcgcggttg tcaccgtaaa acagcctgtc ccattcgcgc atcaccatcg
    14101 ggttgtggta tgcctcgcgc ccgaccatca cgccgtcaac gtgttgcaag tgtccggcga
    14161 ttgcttcgtt ggtggtgatg ccgccgttga tgatgatttc cagctcggga aactcctgct
    14221 tgaggcggta aacgtaatcg tatttcaagg gcggaacatc gcggttttct ttgggcgaaa
    14281 gaccgtccag ccaagcgttg cgggcgtgga cgatgaaggt tttgcaggcg gttttgtcgc
    14341 gcagcgtgcc gacgaaatcg gcaacggttt ggtattcggt ctgcctgtcc acaccgatgc
    14401 ggtgtttgac ggtaacgggg atgccgaccg cgtcgtgcat ggcgttgagg cagtcggcaa
    14461 ccagcccgac ttcgttcatc agacacgcgc cgaacgcgcc tttctgcacg cgcggactgg
    14521 gacagccgca gttgaggttg acctcgttgt agccgtattc ttcggcggct ttggcggctt
    14581 tcgccaaatc ggacggatcg ctgccgccca gttgcagggc gacgggctgc tcgccttcgt
    14641 taaacatcaa aaaacggtct ttgtcgccgt aaacaatcgc gccggcgttg accatttcgc
    14701 tgtaaagcca agcatttcgg gtaatctggc gggcaaggta acggtagtgc ctgtccgtcc
    14761 agtcgagcat cggggcgacg cataaacgcc ttttcccttt atgttcaata agttccttgt
    14821 ttatcataag gttttcacat tcttttaact caaacattct ttattatttg cttggatttt
    14881 tgcccatttc actatatttg acgtaccata cttgcaccat aatttttaat tcttggaatg
    14941 gtgcaaatgg caacaataac caagcggcgc aatccttccg gcgaaacagt atatcgggtt
    15001 caggtacggg tcggcaagaa aggctatcct gctttcaatg agagcaggac gttcagtaaa
    15061 aaggctttgg cggtcgaatg ggggaagaaa cgggaggcgg aaatcgaagc cggcccagaa
    15121 ctgcttttca agcgcggcaa ggtcaagatg atgacgctgt ccgaagctat gcggaagtat
    15181 cttaacgaaa cgcttggagc gggtcggtca aagaaaatgg gcttgcgttt tctgatggag
    15241 ttcccgattg gcggcatcgg catcgataag ctgaaacggt ctgatttcgc ggaacacgtt
    15301 atgcagaggc ggcgcggaat ccccgaactg gacattgcgc caatcgcggc ttctacggca
    15361 ttgcaggagc tgcaatatat ccgttccgtg ctaaaacacg ccttttatgt gtgggggctt
    15421 gaaataggct ggcaggaatt ggatttcgcg gcaaacgggc tgaaacgctc gaatatggtt
    15481 gcgaaatctg caatcaggga cagattgccg accacggaag aactgcaaac cctgacaact
    15541 tatttcctgc ggcaatggca aagcaggaaa tcttccatac cgatgcacct gattatgtgg
    15601 ctggcgattt atacgtcaag gcggcaggat gagatttgcc gcctgctgtt tgacgactgg
    15661 cacaaaaatg attgtacccg gccggttcgt gatttaaaaa atccgaacgg cagcacaggg
    15721 aataataagg agtttgatat tctgcctatg gctttgccgg tcattgacga gctgccggag
    15781 gaatcggtca ggaagcgtat gcttgccaac aaaggcatcg ccgacagcct tgtaccgtgc
    15841 aacggaaaat cggtttccgc cgcttggacg agggcgtgca aggttcttag aattaaagac
    15901 ttgcgcttcc acgatttacg gcacgaggct gctacccgta tggctgaaga cggcttcacg
    15961 attccgcaaa tgcaacgggt aacgctgcat gacggttgga acagcctgca gaggtatgtg
    16021 agcgtacgca aacgctcgac gcggctggat tttaaagagg caatgatgca ggcgcaaagc
    16081 gatataaaat ccgggaagtg atgttaaatt aaaggggatg tgccgcatcc cctgattcct
    16141 ccgtcttgca tcaagaccgg aatttccgat attccgattt tgccttttct tccttgtctg
    16201 cgattgcctt tgcaagctct cggacgctga cgaaatattc cgattttcgg ctgtcggaca
    16261 atcggaaaat cggaaacgga agctcgcacc gtacccccga tgctttaaac gcgttaaagc
    16321 cgatatgggc aaaaaagtct ttgtgtactt ccctcggcgg gatatacggg cggccgccgt
    16381 atgcgattaa aagcccttgt tctttactga tgttcatata tacctctttc tgttaattct
    16441 gctttgtccg ggtttccgct tttttacggc ttggcggatg ttcagcctgc gtcggtgcat
    16501 aagtcctcct gttcgggcgg ttgttcagcc ggccgcccct ccgaaaagtg gcaggctgca
    16561 cgggaagtag tgtttttttc cgtatttctc tgcctgtatc aggttgcaca ggcggtcgca
    16621 aaagccgtca aattcggcat gcggccagcc ttctatctcg ttgaccattt tgctgaacct
    16681 caattcccgg gcaatccgct ttgccgctgc gtcacggcat tcgtgttcca agaggacggg
    16741 gtcttccttt tccttcggcg gtgcaggttc ttgcacggtt tgccacaagt ccggttcgat
    16801 ttgccaagtg tcggcggcaa gcagggtgtc tgttttgcag tcgtacagtt cgcggatgca
    16861 gccgccgtcg gttttgtctt tgtcgataac cagtaaaagc acttcgatcg gcgtgtcttc
    16921 aaacgcgttt tgtatgcggt tgagttctgc cagccggttg ccgatgattt cgcggaatcg
    16981 ctgctcgctt ttgcggtagg cgatgccggg gaacaggatg aagaatccga agcggcgggc
    17041 gttttccaat ccttttaaaa cgaagatttc atcggctacg cccgatttct tccacgggta
    17101 ttcctgtgcg atacggcttt tttcgtcttc gctcaaatcc tttagcttga tggaaaacgg
    17161 cggattcatg acggtgcagt cttgcggttc gccgtcggta taaaggaaaa ggcttgtgtt
    17221 gtatactttg gcggcagggt agttttgcaa caaggcttta cacgcttccg cctgtatctc
    17281 tacggcgcgg aaatcggacg gttcgatgta ctgctccagc tgcccgcttc ctgccgcgcc
    17341 gtcaaatacg cccgggtgtt cgccgcagta tcggcggact ttggcggcaa ccagccgcct
    17401 caagctgccg cccgtgatgt attcggcgta gccgccggct tttttacggt tgttgtgtcc
    17461 ttgaaacgtc atgggttttc gtccgttctc tggcttgtcc gggtgtggcg gagcgtttgc
    17521 ggatgcggct gacgcgctgc ggtttgccgt cttcgccgcg ctgaaaaacg cttgttccgc
    17581 cgcgccggca gccgtgccgc ttatgcctga tgccgtgttt gaagcctttt gtgccggaat
    17641 cgtgatacac gcaggtccgg atgtgcgtcc tcatgccgcc gcttccctgt gcatgaggct
    17701 gatcaggttt gccgtccgtt tgaaatgttc ggtccagttg aagcagctga agccggcgcc
    17761 gttgtcgcaa tgccggatga cgtcttccga gtcttttacg gcccggacca acgcgccgcc
    17821 tttgtccgca acaagcattt tcatcatcgg ccggcgcacg tcgcttatcc gtcgggggcg
    17881 cacgggattc agcggcctgc cgtacatcgc cgaaagctcc ttgtccgcct gtatgcccac
    17941 atcgtaaagc aggttcgcgg cgtgtaggat ccggcctgta aattccgcgt acggcatttt
    18001 aaggcggcgg gcttccgcgc gcgaatccgc gccgttgacg actttgccga ccgccgaaat
    18061 caaaccgccg tcaagttcta aaaacgccat ggctttcttg gcaacccgca cggccaatga
    18121 gtatgccgat acttcggcta tcagccgtgc aggcgcgtct ttcagaaatt cttcgtacgc
    18181 ggcgaagaga tttgacagcg gacgcagcac gagttggcgc tgattcgggc taaggtcgtc
    18241 gaaatcctta gtccattgcg cgaccgcctg cgatgcttcg cggcaggcaa acagtacgct
    18301 ttcctcgttc gcggggtcgt ccgtgttgca gtacaggccc aagcgttgca cctgtttgac
    18361 gatatgttcg gcgaagttga ttaactcctg attgcaggca tagcgcatat cctgcaggg
//
LOCUS       DAWTHD010000032        18321 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWTHD010000032 DAWTHD010000000
VERSION     DAWTHD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18321
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.032"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(214..1494)
                     /gene="hemL"
                     /locus_tag="WM704_000868"
     CDS             complement(214..1494)
                     /gene="hemL"
                     /locus_tag="WM704_000868"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFA9340318.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1558..1733)
                     /locus_tag="WM704_000869"
                     /pseudo
     CDS             complement(1558..1733)
                     /locus_tag="WM704_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            complement(1997..2071)
                     /locus_tag="WM704_000870"
                     /pseudo
     CDS             complement(1997..2071)
                     /locus_tag="WM704_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2268..3596
                     /gene="miaB"
                     /locus_tag="WM704_000871"
     CDS             2268..3596
                     /gene="miaB"
                     /locus_tag="WM704_000871"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFA9340319.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            3614..3778
                     /locus_tag="WM704_000872"
     CDS             3614..3778
                     /locus_tag="WM704_000872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340320.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(3843..5756)
                     /gene="dxs"
                     /locus_tag="WM704_000873"
     CDS             complement(3843..5756)
                     /gene="dxs"
                     /locus_tag="WM704_000873"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFA9340321.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(5841..6758)
                     /gene="xerC"
                     /locus_tag="WM704_000874"
     CDS             complement(5841..6758)
                     /gene="xerC"
                     /locus_tag="WM704_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFA9340322.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6952..8016
                     /locus_tag="WM704_000875"
     CDS             6952..8016
                     /locus_tag="WM704_000875"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HFA9340323.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVISEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            8155..9012
                     /locus_tag="WM704_000876"
     CDS             8155..9012
                     /locus_tag="WM704_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFA9340324.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGDRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            9151..9828
                     /gene="tsaB"
                     /locus_tag="WM704_000877"
     CDS             9151..9828
                     /gene="tsaB"
                     /locus_tag="WM704_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFA9340325.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGGYPATGAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            9825..10265
                     /gene="rimI"
                     /locus_tag="WM704_000878"
     CDS             9825..10265
                     /gene="rimI"
                     /locus_tag="WM704_000878"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFA9340326.1"
                     /translation="MNIRPASPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            10259..10996
                     /locus_tag="WM704_000879"
     CDS             10259..10996
                     /locus_tag="WM704_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFA9340327.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            11058..11699
                     /gene="pyrE"
                     /locus_tag="WM704_000880"
     CDS             11058..11699
                     /gene="pyrE"
                     /locus_tag="WM704_000880"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFA9340328.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GKLSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            11767..12195
                     /locus_tag="WM704_000881"
     CDS             11767..12195
                     /locus_tag="WM704_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340329.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            12192..13502
                     /gene="argA"
                     /locus_tag="WM704_000882"
     CDS             12192..13502
                     /gene="argA"
                     /locus_tag="WM704_000882"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFA9340330.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            13598..15613
                     /locus_tag="WM704_000883"
     CDS             13598..15613
                     /locus_tag="WM704_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HFA9340331.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(15726..16682)
                     /locus_tag="WM704_000884"
     CDS             complement(15726..16682)
                     /locus_tag="WM704_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9340332.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            16918..17889
                     /locus_tag="WM704_000885"
     CDS             16918..17889
                     /locus_tag="WM704_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9340333.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            17922..18245
                     /locus_tag="WM704_000886"
     CDS             17922..18245
                     /locus_tag="WM704_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFA9340334.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT         4103 a         5589 c         5004 g         3625 t
ORIGIN      
        1 cagacggcat ttttacagta gattgaaatc aaaaatgtac acgcccgtca ttcccgcgca
       61 ggcgggaatc cggaaggtcg ggcttgccgt tattttcaat cattacagaa actgaaaggt
      121 ccggattccc gcctgcgcgg aaatgacggc ggtgtgcatt cttacttcaa tctgccaaac
      181 aaatcgaaca gagaaactct gtccgtcaaa acatcatgca gccatcgcct tgaacacttc
      241 aaccgcaacc gcaaccgttt cgtcaatcag ctcgggcgta tgcgcggcgg aaacgaaacc
      301 tgcttcataa gcggacgggc cgaaggcaat gccgcggtcg agcatgccgt ggaagaagcg
      361 tttgaaagcg tcgatattgg agcgcgccat atcggcatag tttcgcggca cgtgtgcggc
      421 gaaatacaga ccgaacatac cgcccacgct gtcggcggca aactcgatac cgtgcgcttt
      481 ggcggcggca ataccgttgg caaggcgttg tgtcaaggcg gtcaggtttt catagaagcc
      541 ttcgcgctgg atgatttcca gcgttttcaa gccggcggcg acggcaatcg ggttgcctga
      601 taatgtacct gcctgataca cgccgcccaa cggggaaata cattccatga tgtctttgcg
      661 tccgccgaac gcggcaagcg gcataccgcc gccgatgact ttgcccatcg tggtcaggtc
      721 gggcgtgatg ccgtgcagcg actgcgcgcc gccgagcgcg acgcggaaac cggtcatcac
      781 ttcgtcgtaa atcaacaccg cgccgtgttt ttcggtcaat ccgcgcaagg ctttgacaaa
      841 ggcttcggtc gggcggacga ggttcatatt gccgacgaag ggttcgacaa tcacgcaggc
      901 gatttcgtcg ccgctttggg caaaggcttc ttcgagttgg gcgatgttgt tgtattcgag
      961 taccaaagta tgtttggtaa agtcggcagg cacaccggcg gaagaaggat tgccgaaggt
     1021 aagcagaccg ctgcctgctt tcaccaacag gctgtcggaa tggccgtggt agcagccttc
     1081 aaatttgatg attttgtcgc ggccggtaaa accgcgtgcc agacggatgg cagtcatcgt
     1141 cgcttccgtg ccggagctga cgaggcgcag ccgttcgaca gacggcataa tttcggcaat
     1201 ttgttcggca atggcgattt cgccttcggt gggcgcgccg aacgacaaac cgcccaacgc
     1261 agcttcgcgc acggcttcga cgacttcggg atgcgcgtgt ccgacaatcg caggccccca
     1321 agagccgaca taatcggtgt agcgcgtgcc gttttcgtcc caaacatacg cgccttcggc
     1381 ttttttgatg aagcgcggca cgccgccgac gctgccgaat gcgcgcacgg gcgaattcac
     1441 gccgccgggg atgatggctt tggcgcggtc gaataaaatt tcgttacggt tcatatttat
     1501 cctcaaatgc cgtctgaacg gcaggtttcg ggcttggaag cagaaagccc cattttatca
     1561 tttttcaggt tgcgacaagg atttgcccgc ttctttgcgg attacgccaa ccgtatcccg
     1621 gatgacggaa cgctcgtctt tttcctcttt atatgtgtaa agcggtagtc ccggacgact
     1681 ccctccccgt cgtaatccac gcaccattcc caatgccgcc gctccgattt catatagatg
     1741 aaattggtcg gcaaaaaatt ataaatcggc aggctgacct cgtgataggc ataacagccg
     1801 aaagggttgc gcttcccgaa acgtgcctct acctccgccc gggtcgtttt gcctttaaca
     1861 accgtttgtg cgattccctc ttccgtctga tatagtggat taacaaaaat caggacaagg
     1921 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg tactggttta
     1981 aatttaatcc actataacgc aggaactgat gttccctgtc gccgaaattg ctggtacacg
     2041 cacacagcag caatgccgcc catacacaca cctcccgtta aagccaaaca ttatacagcc
     2101 gttccgaccg attaaattca tattttaaaa caatattttg cctcccaaac ccacatcgtg
     2161 ctataatccg caccgatttt cagacggcat cgtcgtgccg tctgaaattt tttcattcca
     2221 acaaccatca gccccgcgat tacggctgcc cgagaaagac acaaaccatg aaaaaagtat
     2281 ttatccgcac cttcggctgc cagatgaacg agtacgacag cgaaaaaatg ctgtccgtcc
     2341 ttgccgaaga acacggcggc atcgaacagg ttacccaagc cgacgaagcc gacatcatct
     2401 tgttcaacac ctgttccgtg cgcgaaaaag cacaggaaaa agtgttctcc gacttggggc
     2461 gcgtccgtcc gctcaaagaa aaaaaccccg gcctcatcat cggcgttgcc ggctgcgtcg
     2521 cctcgcaaga aggcgaaaac atcatcaaac gcgcgcctta tgttgacgtg gttttcggcc
     2581 cgcaaacgct gcaccgcctg cccaaaatga ttgtggacaa agaaaccagc gggctgtcgc
     2641 aagtcgatat ttccttcccc gaaatcgaaa aattcgacca cctgccgccc gcccgcgtcg
     2701 aaggcggcgc ggcatttgtg tcgattatgg aaggctgttc caaatactgc tccttctgcg
     2761 tcgtccccta cacgcgcggc gaagaattct cccgcccgct caacgacgta ttgaccgaaa
     2821 tcgccaacct tgcccagcaa ggcgtgaaag aaatcaacct cttgggacaa aacgtcaatg
     2881 cctatcgcgg cgaaatggac gacggcgaaa tctgcgactt cgccaccctg ctgcgtatcg
     2941 tccacgaaat ccccggcatc gaacgtatgc gcttcaccac cagccacccg cgcgagttta
     3001 ccgactcgat tatcgagtgc taccgcgacc tgcccaaact ggtttcccac ctgcacctgc
     3061 cgattcaaag cggttccgac cgcgtattga gcgcgatgaa acgcggctac accgctttgg
     3121 aatacaaatc catcatccgc aaactgcgcg ccatccgtcc tgatttgtgc ctgagcagcg
     3181 acttcatcgt cggtttcccc ggcgagaccg aacgcgagtt cgagcaaacc ttgaaactgg
     3241 tgaaagacat tgccttcgac ttgagcttcg tgtttattta cagcccgcgc cccggcacgc
     3301 ctgccgccaa cctgccggac gacacgccgc acgaagaaaa agtgcgccgc ctcgaagcct
     3361 tgaacgaagt catcgaagcc gaaaccgccc gcatcaacca aaccatgatc ggcacggttc
     3421 aacgctgcct ggtcgaaggc atctccaaaa aagaccccga ccagctccaa gcccgtaccg
     3481 ccaacaaccg cgtcgtcaac tttaccggca cgcccgacat gatcaaccaa atgatcgatt
     3541 tggaaatcac cgaagcctac accttttccc tgcgcggcaa aattgtcgaa gcctaaaacc
     3601 ctcacgccga aaaatgccgc ctgaagcgtt tcagacggca ttttgccttg tatcggcaga
     3661 cggcaccgcc gccgttatac ggacaagcgg aataaatttc caaacgcgca acggcacacc
     3721 aagcaccgcc cgaaagccgc cgcccgaacc cttcctgccg cctgcggggt ttcaataaaa
     3781 aaaccgcaga aacaaacgcc gtttaaaaac gccgcccgat aacgacggcg cggtcaagcg
     3841 gtttaatttg ccgcatcacg gtccggcagc cactcgcgca cccggcgttc caccgcttcg
     3901 gcactcaaac ccaaatcgtc caaaagtttt ttcggatcgc cgtgttcggt tacggtatcg
     3961 gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaacac ttccaagacc
     4021 gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg gtcgtggctt
     4081 cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat atcggcgacg
     4141 gtggcgttca gtttttcggc aaccgccaat gcgggggcga ccatactgcc gaaggcaatg
     4201 aaggcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac ggtttccatg
     4261 ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc ggcgggcgca
     4321 tccgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct cggcgcggca
     4381 acaatcatgt tcggcacaca gcgcaagaag ctcaaatcgt acaagccggc atgggtcggg
     4441 ccgtccgcgc cgacgatgcc cgcacggtcg acggcaaaca aaacgggcag gttttgcagg
     4501 gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata aatcgccacg
     4561 acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg ctgctcggcg
     4621 atgccgacat cgaaatagcg gtcggggaat cgttgttcaa actccaccag tccgctgccc
     4681 tcgcgcatgg cgggggtaat cgcaaccagt cgggaatctg ccgccgcccg gtcgcacagc
     4741 catttgccga atacttgggt ataggtcggt ttggcggcgg gcttgggttc tttttcagac
     4801 ggcatttgcg ccccgccttc tttaggcagg ttggcgacgg cgtggtattt gacggggtcg
     4861 ttttcggcga gtttgtagcc gttgcccttt ttggtgatga cgtgcagcaa ctgagggcct
     4921 ttgcggctgc gcaagtcttt caatacgtcc accagattct cgacgttgtg tccgtccacg
     4981 gggccggtgt agcggaagcc gaaattttca aacagcgaca gcgactgttt ggcgtgttcg
     5041 gcttcttcgg caagggtttt gattttgtgt tcgacttttt gggcaaactc catcgcgccg
     5101 ggtattttgt ctaatacctt gcccgtttgc gctttgacgg tactcaacag tccgtgcata
     5161 tcgcgcacga cgttgctggc aagatatttg ggcaacgcgc cgacgttggg ggaaatcgac
     5221 atttcgttgt cgttgaggac gaccagcaaa tccacatcca tatcgcccgc gcaattcaag
     5281 gcttcaaacg cctgccccgc cgtcatcgcg ccgtctccga tgatggcgac gctgcggcgg
     5341 tcgccgccca agagtttgtc cgccgccgcc atgcccaaag ccgcgccgat ggaggtggag
     5401 gaatgcccca cgccgaacgc gtcgtactcg gactcgcaac gtttcggaaa acccgccaaa
     5461 ccgccgtatt ggcgcatggt gtgcatctgg tttttcctgc ctgtcaggat tttgtgcggg
     5521 tagctttggt gtccgacatc ccacaccagc ttgtcttcgg gcgtgtcgta cacatagtgc
     5581 agggcgatgg tcagttcgac cgcacccaga ttgctggcga aatgcccgcc ggtctgcccg
     5641 acagattcca gcagaaaggc gcgcaactcg ccggcaaggc gcggcagctg ttttttgtcc
     5701 aggcggcgca aatcttgcgg gctgtcaatc aggtcgagta gggggcttgg gttcatggtg
     5761 tgtctttttt atgtgtcgtc cgggtgcaac ggtcaattat atatcaagag cgtgcggctg
     5821 acggctgatt ttgccgtatg tcattcgtcc tgccgcttgg cgcgcgggtg ggcttcgtca
     5881 tagaggcggg cgatgtggtc gaaatcgagc ttggtataaa tctgcgtggt cgaaaggctg
     5941 ctgtgtccga gcagctcctg caccgccctg atgtcgcgcg aagcctgcaa caggtggccg
     6001 gcgtagctgt ggcgcatcat atgcggcgaa acgtgcctgc cgtcgccgta ttgcgccgcc
     6061 cacgattcga ggcgtttttg gatttggcgt tggctcaggc gcgtgccgtt cctgccggta
     6121 aacagggctt tgccgtccga tgccgtctga cgcagcggca gatagttttt caaggcttcc
     6181 acgcttttgc cggtcagcgg aacctgacgc tgcttgcgcc ctttgccggt aacgtgtacc
     6241 cacgcttcgt ccaaatatac atcatctgca ttcaagccgt gtatctcgct cacgcgcaaa
     6301 ccgctgccgt acatcagctc gaacagcgcg tggtcgcgca ccgccagcgg gtcgccgccg
     6361 tccacgggca aatccaacat ccggttcagc cattcctgcg gcagggcttt gggtacgcgc
     6421 tcgggctgct tcggcggttt gatgtcggcg gtcgggtcgg cgcgcatcag cccgcgtttg
     6481 accagccagg cgcaatactg ccgccacgcc gacagcttgc gcgccagcgt ccgaccgtcc
     6541 aaaccgcgct gcgacagccg ccgcaacgcc gccgtaaaat cgcgccgcga caagtcctgc
     6601 ggcacgccgc ctgcatcttc agacggcatt tgtgccaaca gtgcaaacag ttcttccaaa
     6661 tcgcgccggt atgccgcaac cgtgtgctcc gacttgccct cgcgcacgat gttttccaaa
     6721 taagcgtcaa aatacgccgc aaacccgtcc aaaaccataa ccgtcccaca caaatatcaa
     6781 aaaaccagtg aaaacattat cataaacaac acaccaaaat cccgaaccgc ccaaaatccg
     6841 ccccatctgc ccgccgttat cgttaatata aaatcaatga gccgtttatg gttttttgcc
     6901 gtaaaaaaca ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc
     6961 gtatccatgc gccaactgct tgaccacgcc gccgaaaaca gctacggcct gcccgcgttc
     7021 aacgtcaaca acctcgaaca aatgcgcgcc attatggaag ccgccgacca agtcaacgcg
     7081 cccgtcatcg tacaggcgag cgcaggtgcg cgcaaatacg cgggcgcgcc gtttttgcgc
     7141 cacctgattc tggcggcagt cgaagaattt ccgcacatcc ccgtcgtgat gcaccaagac
     7201 cacggcgcat cgcccgacgt gtgccaacgc tccatccaac tgggcttctc ctccgtgatg
     7261 atggacggct ctttgctcga agacggcaaa accccttctt cttacgaata caacgtcaac
     7321 gccacccgta ccgtcgtcaa cttctcccac gcctgcggcg tgtccgtcga aggcgaaatc
     7381 ggcgtattgg gcaacctcga aaccggcgaa gcaggcgaag aagacggagt gggcgcggca
     7441 ggcaaactct cacacgacca aatgctcacc agcgttgaag atgccgtgcg tttcgttaaa
     7501 gataccggcg ttgacgcatt ggcgattgcc gtcggcacca gccacggcgc atacaaattc
     7561 acccgtccgc ccacaggcga cgtattgcgt atcgaccgca tcaaggaaat ccaccaagcc
     7621 ctgcccaata cacacatcgt gatgcacggc tccagctccg ttccgcaaga atggctgaaa
     7681 gtcatcagcg aatacggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc
     7741 gtcgaaggca tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgacct gcgcctcgct
     7801 tccaccggcg cggtacgccg ctaccttgcc gaaaacccgt ccgactttga tccgcgcaaa
     7861 tacttgggca aaaccattga agcgatgaag caaatctgcc tcgaccgtta tcttgcgttc
     7921 ggttgcgaag gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat ggcaagccgt
     7981 tatgccaagg gcgaattgaa ccaaatcgtc aaataacagg ttgcctgtaa acaaaatgcc
     8041 gtctgaacgc cgttcggacg gcatttgatt tttgcttctc tgacctgcct cattgatgca
     8101 gtatgcaaaa aaagatacca taaccaaaac gtttatatat tatctattct gtgtatgact
     8161 aggagtaaac ctgtgaaccg aactaccttc tgctgccttt ctttgaccgc cggccctgat
     8221 tctgaccgcc tgcagcagcg gaggggcgga ggcggtggtg tcgccgccga catcggcacg
     8281 gggcttgccg atgcattaac cgcgccgctc gaccataaag acaaaggttt gaaatcccta
     8341 acattggaag cctccattcc ccaaaacgga acactgaccc tgtcggcaca aggtgcggaa
     8401 aaaactttca aagccggcgg caaagacaac agcctcaaca cgggcaaact gaagaacgac
     8461 aaaatcagcc gcttcgactt cgtgcaaaaa atcgaagtgg acggacaaac catcacactg
     8521 gcaagcggcg aatttcaaat atacaaacag gatcactccg ccgtcgttgc cctacggatt
     8581 gaaaaaatca acaaccccga caaaatcgac agcctgataa accaacgctc cttccttgtc
     8641 agcgatttgg gcggagaaca taccgccttc aaccaactgc ctgacggcaa agccgagtat
     8701 cacggcaaag cattcagctc cgacgatgcc ggcggaaaac tgacctatac catagatttc
     8761 gccgccaaac agggacacgg caaaatcgaa cacctgaaaa cacccgagca gaatgttgag
     8821 cttgcctccg ccgaactcaa agcagatgaa aaatcacacg ccgtcatttt gggcgacacg
     8881 cgctacggcg gcgaagagaa aggcacttac cgcctcgccc ttttcggcga ccgcgcccaa
     8941 gaaatcgctg gctcggcaac cgtgaagata ggggaaaagg ttcacgaaat cggcatcgcc
     9001 gacaaacagt agtatttgaa aaatgccgtc cgaacacgat aatttaccgt tcggacggca
     9061 tttttgcgcc ataccgccct aggacacgac ggcacggcag gcaagccgat ttcactatat
     9121 aatgccgtct gaaccaacga gaaccacacc atgcaagccg atttcaaccg ccccgtcctc
     9181 gccgtcgata ccggaacttc ctatttgtcg ctcgcgctgc gcgccgacgg cgaaatccgt
     9241 ctgttccatc aggaagtcgg catccgccag tccgaactga tcctgccgga aatccgcacc
     9301 ctattccgaa atgcgggcat taccgccgcc gatttggggg cgattgttta tgcgaaaggc
     9361 cccggcgcgt ttaccggact gcgtatcggc atcggcgtgg cgcaaggttt ggcaacgccg
     9421 ttcgacacgc ctttaatcgg catccccacg ctcgatgccg ccgcctcgct gccgccgccg
     9481 caaagctgca tactcgccgc tgcggacgcg cgtatgggcg aagtgtttta tgcgtggttc
     9541 gacacgctga accgccgccg tttgagcgat tatcaagtcg gtagggcggc agacattgcc
     9601 ctccccgagg ggtacgtctt ttcagacggc atcggcagtg cgttcgcact ggaaaaccgt
     9661 ccgcccttct caggcaaacc cgatatgccg accgccgccg attttctcgc gctcgccctc
     9721 ggcggcggct atcccgcaac tggcgcggca cacgccgaac tgctctacgt ccgcaacaaa
     9781 atcgccctga ctgccaaaga acaggcggaa cggaaggcac gaacatgaac atccgcccag
     9841 cctcaccgtc cgactgcgcc gcactggccg cgctcgatac cgtctgcaac ccgtccgcgt
     9901 ggacgcaacg ccaatttgag tccgcgcttg tttcgccgtc cgaacaggtt ttccttgcgg
     9961 aaaaagacgg caggattgcc gcctttatcg tttggcagaa cctgcccgac gaatccgaac
    10021 tgcacctgat tgccaccgcg cccgaatgcc gccgacgggg cgttgcgtcc gccctgctcg
    10081 aatattggta cgcacatctg cccgaaggca cgcaacgcct gctgctcgaa gtccgcgcag
    10141 gcaacgccgc cgcacaggca ctgtatacca aacacggctt cagcattgcg ggcaggcgga
    10201 aaaactatta ctgcgccgcc gacgggcaaa ccgaagatgc cgtcttaatg gagaaaatat
    10261 gttaagcgcg cgctacctcc acctgcacga ggctttgggc ttgggtccga tgtggctgaa
    10321 acaggccgcc gccgtcctgc cgcccaaaaa cacacccgca accccggcac aggcacgtcc
    10381 ccaaaccgtc cgcgccgcca cgatccgccc ttcccaaccc cataacgttc aggcgcggct
    10441 cgaaacgatg aaagcgttgg aaaccgccgc cgtacatacg cgcaaacccg cgcctgaaac
    10501 cgaaacgccc ccgcccggcc tttcagacgg catcgccccc gttcccgccg cttcgggcat
    10561 taccaagctt gccgtcgtca gcctgtgtcc gccgatcgag gatgcggttt acgggcaact
    10621 gttccacggc aaagcgggca tcctgctcga caacatcctc aaagccgcag gactggatgc
    10681 cgcctatgtc cacaaaacct gttgggtgaa aaccgccgcc gtcggtaacc cgatgccgtc
    10741 tgaacaggcc gtcgcggatg cgctgggtca aatcgccggg gaactcgacg gctgccgcgc
    10801 ccccgccgtc ctattcctcg gacaggcgtt tgtcaacctg gaacggcagg cgatgattga
    10861 aactttgtgc ggcagccgtc ccttcttcat catcgaccat cccgcccggc tgttgcgcca
    10921 acccgaactc aaagcccgaa actggcaggt gttgaaacag ttgaaacgcg ccttgcggca
    10981 aggcggcggc agttgaagcg cgccgcacgg ggcggtagaa tcgcaactgc gtcccaatat
    11041 ctgacagaaa gcacaaaatg actgatttcc gccaagattt cctcaaattc tccctcgccc
    11101 aaaatgtttt gaaattcggc gaatttacca ccaaagccgg acggcggtcg ccctatttct
    11161 tcaatgccgg cctcttcaac gacggcgcgt ccacgctgca actggcaaaa ttctatgcac
    11221 aatccatcat tgaaagcggc atccgattcg atatgctgtt cggccccgcc tacaaaggca
    11281 ttattttggc ggcggcaacc gcgatgatgc tggcggaaaa aggcgtgaac gtcccgtttg
    11341 cctacaaccg caaagaagcc aaagaccgcg gcgaaggcgg cgtgttggtc ggcgcgccgc
    11401 ttaaagggcg cgtgctgatt atcgacgacg tgatttccgc cggcacatcc gtacgcgaat
    11461 caatcaaact gattgaagcg gagggtgcaa cccccgccgg tgtcgccatc gcgctcgacc
    11521 gcatggaaaa aggcacgggt aaattgtccg ccgttcagga agtggaaaaa caatacggcc
    11581 tgcccgtcgc ccccatcgcc agcctgaacg atttgtttat cctgttgcaa aacaaccccg
    11641 aattcggaca gttcctcgaa cccgtccgca cctaccgccg gcagtacggc gtagaataaa
    11701 gcatatgccg tccgaaccac ctgccgcttc agacggcatc aaaccgacac acaccgagaa
    11761 aacatcatgc ccgcctgttt ctgtccgcac tgcaaaaccc gcctctgggt caaagaaacc
    11821 cagctcaacg tcgcccaagg cttcgtcgtc tgccaaaaat gcgaagggct gtttaaagcc
    11881 aaagaccatc tggcaagcac gaaagaacct atattcaacg atttgcccga agctgtttcg
    11941 gatgtcaaac tcgtccaccg catcggcacg cacgccatta gcaagaaaca gatttcccgc
    12001 gacgaaatcg ccgatatcct caacggcggt acaaccctgc acgatacgcc gcccgcaacc
    12061 gccgctgccg cacctgccgc cgcaccgcag gtttccgtac cgcccgcccg tcaggaaggg
    12121 ctcaactgga ctattgcaac cctgttcgca cttatcgtcc tcattatgca gctttcctac
    12181 ctcttcatcc tatgaacgcg cccgacagct ttgtcgccca cttccgcgaa gccgccccct
    12241 acatccgcca aatgcgcggc acgacactgg tcgccggcat agacggccgc ctgctcgaag
    12301 gcggcacctt aaataagctc gccgccgaca tcgggctgtt gtcgcaactg ggcatccgac
    12361 tcgtcctcat ccacggcgcg taccacttcc tcgaccgcct cgccgccgcg caaggccgca
    12421 cgccgcatta ttgccggggt ttgcgcgtta ccgacgaaac ctcgctcgga caggcgcagc
    12481 agtttgccgg caccgtccgc agccgttttg aagccgcatt gtgcggcagt gtttcaggat
    12541 tcgcgcgcgc gccttccgtc ccgctcgtat cgggcaactt cctgaccgcc cgtccgatag
    12601 gcgtgattga cggaaccgat atggaatacg cgggcgttat ccgcaaaacc gacaccgccg
    12661 ccctccgttt ccaactcgac gcgggcaata tcgtctggat gccgccgctc gggcattcct
    12721 acggcggcaa aaccttcaat ctcgatatgg tgcaggccgc cgcttccgtc gccgtctcgc
    12781 ttcaggccga aaaactcgtt tacctgaccc tttcagacgg catttcccgc cccgacggca
    12841 cgctcgccga aaccctctcg gcacaggaag cgcaatcgct ggcggaacac gccgccagcg
    12901 aaacccgacg actgatttcg tccgccgttg ccgcgctcga aggcggcgtg catcgcgtcc
    12961 aaatcctcaa cggggccgcc gacggcagcc tgctgcaaga actcttcacc cgcaacggca
    13021 tcggcacgtc cattgccaaa gaagccttcg tctccatccg gcaggcgcac agcggcgaca
    13081 tcccgcacat cgccgccctc atccgcccgc tggaagaaca gggcatccta ttgcaccgca
    13141 gccgcgaata cctcgaaaac cacatttccg aattttccat cctcgaacac gacggcaacc
    13201 tgtacggctg tgccgcactc aaaacctttg ccgaagccga ttgcggcgaa atcgcctgcc
    13261 ttgccgtctc gccgcaggca caggacggcg gttacggcga acgcctgctt gcccacatta
    13321 tcgataaggc gcgcggcata ggcataagca ggctgttcgc actgtccaca aataccggcg
    13381 aatggtttgc cgaacgcggc tttcagacgg catcggaaga cgagctgccc gaaacgcggc
    13441 gcaaagacta ccgcagcaac ggacgaaacc cgcatattct ggtgcgtcgc ctgcaccgct
    13501 gaccgcaacg gaaagccgcc gcagaaatgc cgcctgaacc ccgtttcaga cggcatttcc
    13561 ccgattataa agacctgccc gaccctcaag gaccccgatg aaatcctacc ccgaccccta
    13621 ccgccatttt gaaaacctcg attccgccga aacgcaaaac ttcgctgctg aagcgaatgc
    13681 cgaaacgcgc gcgcgttttt taaacaacga caaggcgcgc gcactttcag acggcatttt
    13741 gaatcaaatg caggacacgc ggcagattcc gttttgtcag gaacaccgcg cgcggatgta
    13801 ccatttccat cagaatgcgg aatatccgaa gggcgtgtac cgcatgtgta cggcggcgac
    13861 ctaccgttcc ggctatcccg agtggaaaat cctgttttcg gtggcggatt tcgatgagtt
    13921 gctcggcgac gatgtgtatt tgggcggcgt gtcgcacttg gtggagcagc ccaaccgcgc
    13981 gctgctgact ttgaacaaat cgggcggcga tacggcgtat acgctggaag tggatttgga
    14041 agcaggggaa ttggtagagg gcggttttca ctttccggca ggcaaaaacc atgtgtcgtg
    14101 gcgcgatgaa aacagcgtgt gggtgtgtcc ggcttgggac gaacgccagt tgaccgaatc
    14161 gggctatccg cgcgaagtgt ggctggtgga acgcggcaag agtttcgagg aaagcctgcc
    14221 ggcgtaccaa atcgataaag gcgcgatgat ggtaaacgcg tggcgttacc tcgatccgca
    14281 gggttcgccg attgatttga ttgaagcgtc ggacggtttt tacaccaaga cgtatttgca
    14341 ggtgtcgtcc gaaggcgggg cgaaaccgtt gaacctgcct aatgattgcg atgtggtcgg
    14401 ctatctggcg ggacatcttt tgctgacgct gcgcaaggac tggcaccgcg cgaaccaaag
    14461 ctatccgagt ggcgcgttgg tggcggtgaa actgaatcgg ggcgaactcg gggcggcgca
    14521 gcttttgttt gcgcccgatg aaacgcaggc attggaaagc gtggaaacga ccaagcgttt
    14581 tgtggtggca agcctgctgg agaatgtaca aggccgtctg aaagcgtggc ggtttgccga
    14641 cagcaaatgg caggaagccg agttgccgca cctgccctcc ggcgcgttgg aaatgaccga
    14701 ccaaccgtgg ggcggcgacg tggtttatct tgccgccagc gatttcacca cgccgctgac
    14761 gctgtttgcg ctggatttga acgtgatgga actgaccgtc atgcgcctcc agccgcagca
    14821 gtttgtttca gacggcatcg aagtgcggca gttttgggcg gtgtcgtccg acggcgaacg
    14881 cattccttat ttccacgtcg gcaaaaacgc cgcgcccgac acgccgacct tagtctatgc
    14941 ttacggaggt ttcggcattc ctgaattgcc gcattatctg ggcagcgtcg gcaaatattg
    15001 gctggaagag ggcaatgcct ttgtattggc aaacatccgc ggcggcggag aattcggccc
    15061 gcgctggcat caggcggcgc agggaatcag caaacacaaa agcgttgatg atttgttggc
    15121 agtcgtgcgt gatttgtccg aacgcggcat gagttcgccc aaacacatcg gcttgcaggg
    15181 cggcagcaac ggcggcctga ttaccgccgc cgccttcgtg cgcgaaccgc aaagcatcgg
    15241 tgcgctggtg tgcgaagtac cgctgaccga tatgatccgt tatccgctgc tgtccgccgg
    15301 ttcaagttgg acggacgaat acggcaatcc gcagaaatac gaagcctgca aacgccggct
    15361 gggcgaattg tcgccgtatc acaatctttc agacggcatc gattatccgc ccgcactcat
    15421 taccaccagc ctcagcgacg accgcgtcca tcccgcccac gcgctcaaat tctacgccaa
    15481 actgcgcgaa acctcgccgc aatcttggct ctactcgcct gacggcggcg gccataccgg
    15541 caacggcacc caacgcgaat ccgccgacaa actcgcctgc gtgttgctgt ttttgaaaga
    15601 atttttggga taagggcagg gggcgacact accgccgcga atgaaaaaag gtcgtctgaa
    15661 attgcttttt cagacgacct tttttaatgg ttgtttcaaa tcaaaatatc tatgccgtcg
    15721 gcccatcagc actttttcac atccgaaggc aaaaatccgt aatgccgcct gaacgcctcg
    15781 ttgaaccgtc ccgcgtggcg gtagccgcaa aagtgcatcg cggcttggat ggtgctgccc
    15841 gattcgatga gggcgagcgc gtgttccagc cgcaggcggc gcaggcgtcc ggcgacggtt
    15901 tcgccggttt gcgctttgaa atagcgtttc aggtagcatt cgttcagccc gacgcggcgg
    15961 gcgatttcgg cgatggtcag cgggcgggcg aattcgctgt tcaaaatatc ggcggcttcg
    16021 tctatgcgcc ggcggcggta gccgttgtcg tggcggcgga aggtgaagcg taataatcgg
    16081 gcggagagtt ccagcgcgtc ggcttcgtcg gcgagcaggc cgaagccgtc cgattcgaac
    16141 gggcgttgca gcagggggca ggccgccgcc gtcagtgtcc ccgcgttttg cgccagccgt
    16201 tgcagggcga atcggcctat cgtttgcggc gaaaacaggc gttcgtccag taaaccttcg
    16261 tcgtgccagc ggcgcagttt ttccagcgaa aaatccaaat gcagcgcgca catgccgccg
    16321 ctgtctggca gcagggtttc ggatacgtcc gccagttcgc cgcgtaccag ccagatttcg
    16381 ccggcagacg ggcggtattc cctgccgccc atttgtaacc ggttctgccc cgataccatg
    16441 acgaacaagg cgcagttgtg gctgaaattg tggatttcgg tgggaaacgc acccgttccg
    16501 ccgccgcgca tccgtgataa ggtgatgccc gagtcgaagc ggttgatgca cattttcaga
    16561 tgcaaaccgg gctgttttgc ctgcgcaatg agtgcgctgt cggaacagcc gtccaacgcc
    16621 cagccggatt tatcggagcg gacataggtt tggtactggc ggtagatggc ggcggtgttc
    16681 atgattggat aggaacgagt tgtctaacaa atgaattaaa taggaattat taccaataat
    16741 caagcacagg gattggttgg aacggaaaag gtcgtctgaa agggtgtttc agacaacctt
    16801 ttccgtttcg ggaatttgtt ttgccgtatc gggaattttg cgttttgcgg cgtggtttct
    16861 gcaggttgtt tgcttaataa taaacattct tattcgtatg caaaggaacc gcacaccgtg
    16921 aaaccgcgtt tttattgggc agcctgcgcc gtcctgccgg ccgcctgttc gcccgaacct
    16981 gccgccgaaa aaactgtatc cgccgcatcc caagccgcat ccacacctgt cgccacgctg
    17041 accgtgccga ccgcgcgggg cgatgccgtt gtgccgaaga atcccgaacg cgtcgccgtg
    17101 tacgactggg cggcgttgga tacgctgacc gagccgggcg tgaatgtggg cgcaaccacc
    17161 gcgccggtgc gcgtggacta tttgcagcct gcatttgaca aggcggcaac ggtggggacg
    17221 ctgtttgagc ccgattgcga atccctgcac cgccacaatc cgcagtttgt cattaccggc
    17281 gggccgggtg cggaagcgta tgaacagttg gcgaaaaacg cgaccaccat agatttgacg
    17341 gtggacaacg gcaatatccg caccagcggc gagaagcaga tggagaccct gtcgcggatt
    17401 ttcggtaagg aagcgcgcgt ggcggaattg aatgcgcaga ttgacgcgct gttcgcccaa
    17461 aagcgcgaag ccgccaaagg caaaggacgc gggctggtgc tgtcggttac aggcaacaag
    17521 gtgtccgcct tcggcacgca atcgcggttg gcaagttgga tacacggcga catcggcctg
    17581 ccgcccgtgg acgaatcttt acgcaacgaa gggcacgggc agcccgtttc cttcgaatac
    17641 atcaaagaga aaaaccccgg ctggattttc atcatcgacc gcaccgccgc catcgggcag
    17701 gaagggccgg ctgccgtgga agtgttggat aacgcgctgg tatgcggcac gaacgcttgg
    17761 aagcgcaagc aaatcatcgt catgcctgcc gcgaactaca ttgtcgcggg cggcgcgcgg
    17821 cagttgatac aggcggcgga acagttgaag gcggcgtttg aaaaggcaga acccgttgcg
    17881 gcgcagtagt tttattcaaa acaaaccaca aggagaaccc catgcaaaac gaaaccatca
    17941 acctgaaaca gcaccttgcc gccatcaaag aatactggca gcccgaaatc atcaaccgcc
    18001 acgggttcca attccgcctg gtcaaacttt tgggcgatta cggctggcac acgcacggat
    18061 acagcgacaa agtgctgttt gccgtggagg gcgacatggc ggtggacttc gccgacggcg
    18121 gcaacatgac gatacgcgag ggcgagatgg cggtcgtgcc gaagtcggtg tcgcaccgcc
    18181 cgcgttcgga aaacggctgc tcggtggtgc tgattgatct gcccgacccg tccgaggccg
    18241 cctgaaaacg aagtttccga aggaagctga gtttctgcga agctaaaagc agcctacacc
    18301 ttcaatatgc caaaagcata g
//
LOCUS       DAWTHD010000033        18261 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWTHD010000033 DAWTHD010000000
VERSION     DAWTHD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.033"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            331..1029
                     /locus_tag="WM704_000887"
     CDS             331..1029
                     /locus_tag="WM704_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFA9340335.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1086..2090)
                     /locus_tag="WM704_000888"
     CDS             complement(1086..2090)
                     /locus_tag="WM704_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFA9340336.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2160..4565
                     /locus_tag="WM704_000889"
     CDS             2160..4565
                     /locus_tag="WM704_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFA9340337.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQASDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSQDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4667..5668
                     /locus_tag="WM704_000890"
     CDS             4667..5668
                     /locus_tag="WM704_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9340338.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6149..8029)
                     /locus_tag="WM704_000891"
     CDS             complement(6149..8029)
                     /locus_tag="WM704_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFA9340339.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQEGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGLFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8185..8817
                     /locus_tag="WM704_000892"
     CDS             8185..8817
                     /locus_tag="WM704_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFA9340340.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8889..10259
                     /gene="purB"
                     /locus_tag="WM704_000893"
     CDS             8889..10259
                     /gene="purB"
                     /locus_tag="WM704_000893"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFA9340341.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTHGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10339..12207
                     /locus_tag="WM704_000894"
     CDS             10339..12207
                     /locus_tag="WM704_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFA9340342.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTIELSTVFLSNIYLGFRLGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRALRHISIILFLILTATSFLPAAFYTDILNQPNTYYLSTLRFPELLVGSLLAVY
                     GQTQNGRRQTENGKRQLLSLLCFGALLVCLFVIDKHDPFIPGITLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFISFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLMLVGYNLYSRGILKQEHLRPLPG
                     TPVAAENNFPETVLTLGDSHAGHLRGFLDYVGGREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFKARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAINQYLRPIRAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPENTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
     gene            complement(12343..12957)
                     /locus_tag="WM704_000895"
     CDS             complement(12343..12957)
                     /locus_tag="WM704_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFA9340343.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGI"
     gene            complement(12993..15143)
                     /gene="dinG"
                     /locus_tag="WM704_000896"
     CDS             complement(12993..15143)
                     /gene="dinG"
                     /locus_tag="WM704_000896"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFA9340344.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15325..16623)
                     /locus_tag="WM704_000897"
     CDS             complement(15325..16623)
                     /locus_tag="WM704_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFA9340345.1"
                     /translation="MPSETALPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16613..17524)
                     /locus_tag="WM704_000898"
     CDS             complement(16613..17524)
                     /locus_tag="WM704_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFA9340346.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17632..18030
                     /locus_tag="WM704_000899"
     CDS             17632..18030
                     /locus_tag="WM704_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFA9340347.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18079..>18261
                     /locus_tag="WM704_000900"
     CDS             18079..>18261
                     /locus_tag="WM704_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFA9340348.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEEHD"
BASE COUNT         3914 a         4989 c         5252 g         4106 t
ORIGIN      
        1 ttaatccact atatttgaaa ggggtgtaac ccttccaaat cagggcaacg catagggcgg
       61 tgctttatgt gccgtcctgt gtgttgaaac atcattgttc cccaacagcc gtatccgtcc
      121 ggggcggcgc gtattgtacg acagaaaccg cttcggatgg catcgggttt gatgccgtct
      181 gaagcggttt cccgaaacga aaacgtcctt tttcggcggc aaactgtatc aacacgcgaa
      241 aatacaataa aataccaata tccgatttat ttccaacatc acggataaaa taccaatatc
      301 cgatttattt ccaacatcac ggagagccgt atgaaatcca gacacctcgc cctcggcgtt
      361 gccgccctgt tcgcccttgc cgcgtgcgac agcaaagtcc aaaccagcgt ccccgccgac
      421 agcgcgcctg ccgcttcggc agccgccgcc ccggcaggac tggtcgaagg gcaaaactac
      481 accgtccttg ccaacccgat tccccaacag caggcaggca aggttgaagt gcttgagttt
      541 ttcggctatt tttgtccgca ctgcgcccgc ctcgaacctg ttttgagcaa acacgccaag
      601 tcttttaaag acgatatgta cctgcgtacc gaacacgtcg tctggcagaa agaaatgctg
      661 ccgctggcac gcctcgccgc cgccgtcgat atggctgccg ccgaaagcaa agatgtggcg
      721 aacagccata ttttcgatgc gatggtcaac caaaaaatca agctgcaaga gccggaagtc
      781 ctcaaaaaat ggctgggcga acaaaccgcc tttgacggca aaaaagtcct tgccgcctac
      841 gaatcccccg aaagtcaggc gcgcgccggc aaaatgcagg agctgaccga aaccttccaa
      901 atcgacggta cgcccacggt tatcgtcggc ggcaaatata aagtcgaatt tgccgactgg
      961 gagtccggta tgaacaccat cgaccttttg gcggacaaag tacgtgaaga acaaaaagcc
     1021 gcgcagtagg cccgtttgaa aaatgccgtc tgaaacttgg ttttcagacg gcattttgat
     1081 tgggtttaga acgtaaagcc cgtttccagt tcttcatcgc cgaccagttc gaccaagagc
     1141 gcgtagagcg gggcgagttc ggcataacgg cgcgatacgc ggcgcagata gtttaagaaa
     1201 cgcgggattt ccggacggta tttgtctttg ccgtcgcggt agtacaggcg tgcgaagatg
     1261 cccgcgacct tcaagtgccg ctgcacgccc atccattcga accggcggta aaactcgtca
     1321 aactcggcag ggacaggcaa gccggcagcc cgcgcctttt cccagtagcg gataaccaaa
     1381 tccaaaacaa actcttcttc ccactcgata aaggcatcgc gcaacagcga caccaaatcg
     1441 taggaaatcg ggccgtaaag cgcgtcttgg aagtctaaaa cgcccggcct gccgcgcgtc
     1501 agcatcaggt tgcggacgat aaagtcgcgg tgcacgtaga ctttgggctg ggccaacaag
     1561 ggcggcagca gcgtatcggc ggtttgctgc caaagttggc gttgtttgaa tgttaattcg
     1621 cgccccaatt cttttgcgac aaaccattcc gggaacaggt tgatttcgcg cagcatcgtt
     1681 tcacggtcat attcgggcaa aaccccttca cggcttgcct tctgcaatcc gaccagctcg
     1741 ccgatggcct ccagcaacag ggctttgtgt gccgcttcgc cctgctcctg aagcatcgcg
     1801 gtcaaaaacg tcgtattgcc caagtcgttc aataccacaa accccaaatc cgtgtccgcg
     1861 tgcaatacct gcggcacatt gaccatgtca aacagcttct gcaccttcaa ataaggcgcg
     1921 acactcatct tgtcgggcgg cgcatccata cagacgacgc tgccgccgtc tgaaaacgcc
     1981 gcacggaaat agcggcggaa atcagcatcc gccgccgcga aggacagatc gaagtcccgt
     2041 tcgggataaa cggtctgaag ccaatttttc agttcggttt gccgttgcat aacagtatcc
     2101 gggcatttcg ggttacaata aacactattc taaccggtaa accgacttga ggggcgattt
     2161 tggctcgttt attttcactc aaaccactgg tgctggcatt gggcttctgt ttcggcacgc
     2221 attgcgccgc cgataccgtt gcggcggaag aggcggacgg gcgtgtcgca gaaggcggtg
     2281 cgcagggcgc gtccgaatcc gcacaagctt ccgatttgac cctcggttcg acctgcctgt
     2341 tttgcagtaa cgaaagcggc agccccgaga gaaccgaagc cgccgtccaa ggcagcggcg
     2401 aagcatccgt ccccgaagac tatacgcgca ttgttgccga caggatggaa ggacagtcgc
     2461 aggttaaggt gcgcgcggaa ggaagcgtta tcatcgaacg ggacggcgca gtcctcaata
     2521 ccgattgggc ggattacgac cagtcgggcg acaccgttac cgtaggcgac cggttcgccc
     2581 tccaacagga cggtacgctg attcggggcg aaaccctgac ctacaatctc gatcagcaga
     2641 ccggcgaagc gcacaacgtc cgtatggaaa ccgaacaagg cggacggcgg ctgcaaagcg
     2701 tcagccgcac cgccgaaatg ttgggcgaag ggcgttacaa actgacggaa acccaattca
     2761 acacctgttc cgccggagat gccggctggt atgtcaaggc cgcctctgtc gaagccgatc
     2821 ggggaaaagg cataggcgtt gccaaacacg ccgccttcgt gttcggcggc gttccccttt
     2881 tctatacgcc ttgggcggac ttcccgcttg acggcaaccg caaaagcgga ctgctcgtcc
     2941 cgtccgtatc tgccggttcg gacggcgttt ccctttccgt cccctattat ttcaaccttg
     3001 cccccaactt cgatgccact ttcgcccccg gcattatcgg cgaacgcggc gcgacgtttg
     3061 acggacaaat ccgttacctg cgtcccgatt acagcggaca gaccgacctg acctggttgc
     3121 cgcacgataa gaaaagcggc aggaacaacc gctatcaggc aaaatggcag caccggcacg
     3181 acatttccga cacgcttcag gcgggtgtcg atttcaacca agtctccgac agcggctact
     3241 accgcgactt ttacggcggc gaagaaatcg ccggcaacgt caacctcaac cgccgcgtat
     3301 ggctggatta tggcggcagg gcggcgggag gcagcctgaa tgccggcctt tcggttcaga
     3361 aataccagac gctggcaaac caaagcggct acaaagacga accttacgcc atcatgcccc
     3421 gcctttctgc cgattggcat aaaaacgcag gcagggcgca aatcggcgtg tccgcacaat
     3481 ttacacgctt cagccaagac ggccgccaag acggcagccg actggtcgtg tatcccggta
     3541 tcaaatggga tttcagcaac agctggggct acgtccgccc caaactgggg ctgcacgcca
     3601 cttattacag cctcgacagt ttcggcggca aagcatcccg cagcgtcggg cgcgttttgc
     3661 ccgttgtcaa tatcgacggc ggcacaacct tcgaacgcaa tacgcgcctg ttcggcggcg
     3721 gagtcgtgca aaccatcgag ccgcgcctgt tctacaacta tattcctgcc aaatctcaaa
     3781 acgacctgcc caatttcgat tcgtcggaaa gcagcttcgg ctacgggcag cttttccgcg
     3841 aaaaccttta ttacggcaac gaccgcatca acgccgccaa cagcctttcc accgccgtgc
     3901 agagccgtat tttggacggc gcgacggggg aggagcgttt ccgcgccggt atcggtcaga
     3961 aattctattt caaggatgat gcggtgatgc ttgacggcag cgtcggcaaa aatccgcgca
     4021 gccgttccga ctgggtggca ttcgcctccg gcggcatagg cgggcgtttc accctcgaca
     4081 gcagcatcca ctacaaccaa aacgacaaac gcgccgaaca ttacgccgtc ggcgcaggct
     4141 accgccccgc ccccggaaaa gtgttgaacg cccgctacaa atacgggcgc aacgaaaaaa
     4201 tctacctgca ggcggacggt tcctattttt acgacaaact cagccagctc gacctgtccg
     4261 cacaatggcc gctgacgcgc aacctgtctg ccgtcgtccg ctacaactac ggttttgaag
     4321 ccaaaaaacc gatagaaatg cttgccggtg cagaatacaa aagcagttgc ggctgctggg
     4381 gcgcgggcgt gtacgcccaa cgctacgtta ccggcgaaaa cacctacaaa aacgccgtct
     4441 ttttttcact tcagttgaaa gacctcagca gcgtcggcag aaaccccgca ggcaggatgg
     4501 atgtcgccgt tcccggctac atccccgccc actctctttc cgccggacgc aacaaacggc
     4561 cctgaccgtc ggaaacccgg caggagcacc gttcctgcac aagatagcat tccaccgaca
     4621 accccaaacc cgccatcaaa ggcaggattc aaacgataag gaaagaatga tgaaaatcaa
     4681 agccctgatg attgccgccg cattgctggc agcagccgat gtccacgccg caccgcaaaa
     4741 ggcaaaaacc gcgcccgcca aagcggtcaa agctgccgcc acggcgcaaa aagaagccgc
     4801 acccgcacaa cagcagggcg gtatccgctt ttcagacggc attgccgccg ttgccgacaa
     4861 cgaagtcatc accaaccgcc ggcttgccga agccgttgcc gaagccaaag ccaccctgcc
     4921 cgaagacgcg cagataagtg aatccgagct gtcccgacag gtgctgatgc agcttgtcaa
     4981 ccaatccctg attgtacagg cgggcaaacg ccgcaacatt caggcaagtg aagcggaaat
     5041 cgatgccgtc gtcgcacaaa atcccgccct caaaaacctc agccccaccc aacgccgcga
     5101 gcttgccgac aacatcattg ccgaaaaagt ccgccagcag gcagtgatgc aaaacagccg
     5161 cgtgagcgaa gctgaaattg atgccttcct cgagcaggcg caaaaacaag gcatcaccct
     5221 gcccgaaggc gcaccgttgc gccaataccg cgcccaacac atcctgatta aagccgacag
     5281 caaaaacgcc gccgtcggcg cggaaagtac catccgcaaa atctacgacc aggcgcgcaa
     5341 cggcacggat ttcgcaggtt tggcacgccg atattcccaa gacgcaagcg cgggcaatgg
     5401 cggcgacttg ggctggtttg ccgacggcgt aatggttccc gcctttgaag aagccgtcca
     5461 cgcgctcaaa cccggacagg tcggcgcgcc tgtccgcacc caattcggct ggcacatcat
     5521 caaattgaac gaagtgcgcg atgccggcac gccggaggaa cgtatccgca attccgtgcg
     5581 gcaatacatc ttccaacaaa aagccggaca ggcaaccgtc aacctgttgc gcgacctgca
     5641 ttccggcgcg tatgtcgaca tccgctaagg cggtttgaat ccaaaagtca taccgatcgg
     5701 gcaaaagtcc gggcggtatg gcttttttga atttcgagtt acttttacac cgccattcgt
     5761 cattcccgcg caggcgggaa tctagcaacg aaaagtaaca ggaatttatc gggaatggcc
     5821 ggagtttaaa ggtctggatt cccgccttcg cgggaatgac gattcagaag ttacacgaaa
     5881 ctcaaaaaaa aaaaacgaaa ccgaacgaac cggattcccg cttttacggg aatgacggca
     5941 tatcggtttc cgtgaggaca aacctagatt cccgcctgcg cgggaatgac gggattttag
     6001 attgcgggta tttatcggga atggcgattt aaaaattacc taaaactaaa cttaaaagca
     6061 actgaaactt aaaaaactaa aatttcgcct gcgtgggaag gatgggattt cagatttctg
     6121 tttttgtggg aatttgccgt atcgggcttc agacggcatt gcctgccgtt ttgcccccgg
     6181 gtgcggctgc cttgatgtag ttgagcgaga caaactgctt ctcggcatcc aattcggtga
     6241 ttttaaacaa tgcctgcgat ttgggcagcg cgtcaaacgg aataccggtc gcgcgcgttg
     6301 tcagtggcag accttcgatg cggacgaggt cttctttcag aatcgtcgcg gtcagttcgc
     6361 tgatgccttg ttgttgcagg tacacgaggc tccagtaggc ttccatctgc cgttggaagt
     6421 cggcgtaggc ggcgtaggcg gtgtcgaaat cgcgcagggc ggcgaagagc gcggcatcgc
     6481 ttttttgata cagcggctcg gcagtgtcgt cgatcaggct gatcagctgc ttttggttga
     6541 tgtagtcggc ggcgcggcgc agcggcgagg taaaccagcc gtaatgctgc acgcccatgc
     6601 cgatgtgcgg ctcggattgg gtgctcatgc gcactttgcc tgccggttgg acgcggaaaa
     6661 ggccgggcag cccgttttcg tctagcattt gcgcccaagt gctgttggca agaatcatca
     6721 tctcgctgac cagcgtatcg atgggcgaac cgcgttcgcg gcggacgacg gagacgttgc
     6781 cttcttcatc caattcgatg ctgtaatcgt attgcggcgc gcggtcgggt tcgtatttgc
     6841 cgcgcgcttt ttgcagggcg gtggcgaatt gatagaacca aatcaggtct tgatggtggg
     6901 cgaacatcat ttcgcctgct tggtccaagc cggtttctgc gttgaaatgc ggctcgatgg
     6961 cttggatacg caggtttgcg gcgatgttga cggcttcgat tttgcaggtc ggctcgccga
     7021 cgttgaactc gccgtccaca tcgaaataaa tgctgacggc agggcggtat gcgcctgcat
     7081 cgaggctgaa cgcggtaatc cagttttcgg gcagcatcgt gattttgccg ccggggaaat
     7141 aaaccgtact caaccgctgc ataatgattt gttccattcc gccgccttgt cggacggcaa
     7201 gcgacggcgc ggcgatgtgg atgccgacgc gcttcgtgcc gttgcccaaa tcggtcaggc
     7261 tcaaggcatc gtccacttcg gtggtcgatt cgtcgtcaat ggaaaaggcg gtaacgtcgg
     7321 ccttgggcag gtcgggcatt tcgggaaggg agaggtcggg gaaacctgtt cctttgggga
     7381 agtatttgat ttcaaagcct tcttgcaggt attgcggaat ggacgtaatg ccgcccgttt
     7441 ttttcgccag ttcgtaggcg gaggttttca gtgcgtcggc ggctttggta aaggctttgt
     7501 aggtcagcga ctgtttgtcg ggcgcgtgta agatggttct caaatccgcc gcgatttcag
     7561 acggcatctc gccgcgtttc aaggcttccg cccaagcgtc gatttgcgcg tcttgctgtt
     7621 ttttgcgttc gatggcggca agcgcttgtt tcaaagtttc ttcgggcgcg gctttgaaca
     7681 cgcctttggc ttttttgtag aaatacaccg gcgcggcgta cagcgcaatc aaagtcgccg
     7741 ccagctcggt tttggtcggc gcatggccgt aatattcttc ggcgatggct tcggcggtaa
     7801 actcctcttc gccgcatact tcccacaata aatcggtgtc gatgtccgcc gcctgcgcct
     7861 gagcgttttc caaaaacgcc gccatatcgc cgtcaaactc ggcaaagaca ttgtttgcct
     7921 tcactttggt gcgtttgccg tgttgggtat cgacttggta ggtagcgtcg tttttttgga
     7981 cgacgacggc aactttgaac tggccggact cttcgtaaaa aatattcatt tttcggattt
     8041 ttctgtggaa actcaagcgg gcgattttag cagattaccg aaaatgccgt ctgaaaaaag
     8101 gttgggggaa ggcgggtgcg gctttgcggt gcttgcgttg cgtttttgac ggggtacaat
     8161 cgccgttttt gaacggagga cggaatggag aatgtaaacc gcgtgccgga gcaggcacgt
     8221 tatgatgccg aacgcaggca ggcagacgaa gcattggcgg acgtgtttcc ggcagtcagt
     8281 attttcggca gcgcgcgcac gccgcaggat catgcggatt atgcgtttgc ctgccgtctg
     8341 gcgcggcggc tgtcggattc gggtattgcc gtcatttcgg gcggcgggcc ggggattatg
     8401 gaggcggcaa acaagggcgc gtttgccggg aagtcggttt cggtagggct gaacattgcc
     8461 ctgccgcacg agcagaaacc gaatccgtat caggacatcg ccttgcggtt ttcccgtttt
     8521 gccgaacgca aggcagtgtt tttccgctat tcccaagcat atgtcgtgat gccgggcggc
     8581 ttcgggacgc tggacgaatt gtttgaaatc ctgaccttgg tgcagacggg caaagtgccg
     8641 ccgcgtccgg tcgttttggt tggaaaggcg ttttggtcgg gcttggcgga gtggataaac
     8701 gcgcagcttt tggcgcgcgg tatgatttcc gaaggggccg cttctttgtt ctccatatcg
     8761 gacgacgaag acgaaatcat tgcgtatctg tcggaacacg ggcttcagac ggcatagcgt
     8821 cctgagagtg atgtataatt gcaaacaatt tgtcaatttt tgatgtcttt tccgaacagg
     8881 atgccgatat gatcaacccc atcgcctcgc tttcccccct agacggccgt tatgcccaat
     8941 ccgttgaagc attgcgcccg attttttcag agtacggact gatgaaggcg cgcgtcaaag
     9001 tcgaattaaa ctggctcaaa gccctggccg ccgagccgga gattgccgaa gtgccgccct
     9061 tcagtgccga aacgcttgcc gaaatcgaca aagtcatcga aaacttttca ttagaagacg
     9121 cggccgccgt caaagccatc gaagccacga ccaatcatga tgtcaaagcc atcgaatatt
     9181 ggctgaaaaa acgttttgcc gaagtgccgg aagtcgccgc cgtgagcgag ttcatccact
     9241 tcgcctgcac cagcgaagac atcaacaacc tgtcccacgc cctgatgctg caagaggcgc
     9301 gcgaaaccgt catcctgccc aaacttgccg aaatcatcgg caaactcacc gctatggcgc
     9361 acgaccttgc cgccgtcccg atgatgagcc gcacccacgg ccagcccgcc acaccgacca
     9421 ctttgggcaa agaaaccgcc aatgtcgtgt accgcctgca acgccagttc aaaatccttc
     9481 aggcgcaaga attcctcggc aaaatcaacg gcgcggtcgg caactacaac gcccacatgg
     9541 ccgcctatcc cgatgtggat tgggaaacac actgccgcaa cttcgtcgaa atcagcctcg
     9601 gcctgacctt caacccctac accatccaaa tcgagccgca cgactatatg gcggagtttt
     9661 tccaaaccct cagccgtgtc aacaccatcc tgatcgactt taaccgcgac gtttggggtt
     9721 atatttcatt gggttatttc aaacaaaaag tcaaagcggg cgaagtcggt tcttccacca
     9781 tgccgcacaa agtcaacccc atcgactttg aaaactccga gggcaacctc ggcatggcaa
     9841 acgccgtatt gggctttttg tccgaaaaac tgccgatttc ccgctggcag cgcgacctga
     9901 ccgacagcac cgtattgcgc aatatgggcg tgggcgtggg ctatgccgtg ttgggtttcg
     9961 ccgcccacct gcgcggtctg aacaagctcg aacccaaccc tgccgcgctt gccgccgatt
    10021 tggatgtcac ttgggagctg ctcgccgagc cgattcaaac cgtaatgcgc cgttacggtg
    10081 tcgccaatcc ttacgagaag ctgaaagacc tgacgcacgg taaagacggc attacgcccg
    10141 aagtgttgaa actctttatc gaatcgttgg aaattccggc agaagccaaa gccaaattgc
    10201 ttgagctgac ccctgcgttg tatgtgggca aggctgaagc gttggcgaaa cggatttgag
    10261 tgcttattga aaccgatgcc gtccgaacgc gcgttcagac ggcattttta agataacggg
    10321 acatacgggg cgatattgat gcaagctgtc cgatacaggc ctgaaattga cggattgcgg
    10381 gccgtcgccg tgctatccgt cattattttc cacctgaata accgctggct gcccggagga
    10441 ttcctggggg tggacatttt ctttgtcatc tcgggattcc tcattaccgg catcattctt
    10501 tctgaaatac agaacggttc tttttctttc cgggattttt atacccgcag gattaagcgg
    10561 atttatcctg cttttattgc ggccgtgtcg ctggcttcgg tgattgcctc tcaaatcttc
    10621 ctttatgaag atttcaacca aatgaggaaa accatagagc tttctacggt tttcttgtcc
    10681 aatatttatt tggggttccg attggggtat ttcgatttga gtgccgacga gaaccccgta
    10741 ctgcatatct ggtctttggc agtagaggaa cagtattacc tcctgtatcc ccttttgctg
    10801 atattctgct gcaaaaagac aaaatcgcta cgggcgttgc gccacatcag catcatcctg
    10861 ttcctgattt tgactgccac atcgtttttg ccggccgcgt tttataccga catcctcaac
    10921 caacccaata cttattacct ttcgacactg aggtttcccg agctgttggt gggttcgctg
    10981 ttggcggttt acgggcaaac gcaaaacggc agacggcaaa cagaaaatgg aaaacggcag
    11041 ttgctttcat tactctgttt cggcgcattg cttgtctgcc tgttcgtgat cgacaaacac
    11101 gatccgttta tcccgggaat aaccctgctc cttccctgcc tgctgacggc gctgcttatc
    11161 cggagtatgc aatacgggac acttccgacc cgcatcctgt cggcaagccc catcgtattt
    11221 gtcggcaaaa tctcttattc cctatacctg taccattgga tttttatttc cttcgcccat
    11281 tacattacag gcgacaaaca gctcggattg cctgccgtat cggcggttgc cgcgttgacg
    11341 gccggatttt ccctgttgag ctattatttg attgaacagc cgcttagaaa acggaagatg
    11401 accttcaaaa aggcattttt ctgcctttat ctcgccccgt ccctgatgct tgtcggttac
    11461 aacctgtatt caagagggat attgaaacag gaacacctcc gcccgctgcc cggcacgccc
    11521 gttgctgcgg aaaataattt tccggaaacc gtcttgaccc tcggcgactc gcacgccgga
    11581 cacctgcggg gttttctgga ttatgtcggc ggcagggaag ggtggaaagc taaaatcctg
    11641 tccctcgatt cggagtgttt ggtttgggtg gatgagaagc tggcagacaa cccgttgtgc
    11701 cgaaaatacc gggatgaagt tgaaaaagcc gaagccgttt tcattgccca attctatgat
    11761 ttgaggatgg gcggccagcc cgtgccgaga tttgaagcgc aatccttcct gatacccggg
    11821 ttcaaagccc gattcaggga aaccgtcaag aggatagccg ccgtcaaacc cgtctatgtt
    11881 tttgcaaaca atacatcaat cagccgttct cccttgaggg aggaaaaatt gaaaagattt
    11941 gctataaacc aatacctccg gcctattcgg gctatgggcg acatcggcaa gagcaatcag
    12001 gcggtctttg atttggttaa agatattccc aatgtgcatt gggtggacgc acaaaaatac
    12061 ctgcctgaaa acacggtcga aatacacggc cgctatcttt acggcgacca agaccacctg
    12121 acctatttcg gttcttatta tatggggcgg gaattccaca aacacgaacg cctgcttaaa
    12181 tcttcccgag gcggcgcatt gcagtagcct gccttcttgc cggatattgc ctttggcagc
    12241 ctatgccgct gtttgccctt tgaggcggct tttattttgc cgttttgagg ccggggtcgg
    12301 gaataaccgt tttttgatga ttttccctcc ccggctgtgt catcaaattc ctagttgcct
    12361 ttccaaactc tccaccagat tgtcatccag tttcaaagcc tgcgacaggc gggcgaggaa
    12421 gacggtttct ttccgcgaca aatcggcaca gaccaacctt gccgccagat aggtctccgc
    12481 cgccaacgcc tcatcgttgc cgacggcggc ggcgatgtcc ccgatgcttg cgggcaggcg
    12541 gtattcggcg gcgagccatg cggcagtttc ggggtctgtg ccgctttcct gttcgatagt
    12601 ccggcgttcg gcttcgtcta tcatgccgtc tgaagcggcg gcggctatca tggtacgcaa
    12661 tacggtgcgg ctgtatgttt cttcggtttt tccggcaggt tggaaatcgc tttgtgttac
    12721 ggttgcccgc cctttgtttt gctgccacat ctgatagccc cggtaggcga ggtagcccaa
    12781 agcggcggtc gaaccgattt tggtgatggt tttgcggttt ttaccgttca gcagcatgga
    12841 ggcgacaccg gcaaccagcg cgcctccgcc gaatgaattg agcgggctgc cggagaatgt
    12901 gctgcctttt ttttgaaccg tgcttaagac ttggttgagc aaccgggtga agttcatgat
    12961 ttttcctttc tgttgcggga agggcggtat gtttacccta tccttttaaa cggtggcagg
    13021 ccggccaata attgttggcc gtaccgctgc gttttgatgc ggttgtcgag gatggttacg
    13081 cggccgtagt cttgttcggt gcggatgagg cggccgacgg cctggatgag tttgatgccg
    13141 gcttcgggga cggtgatttc gatgaagggg ttgccgccgc gttgttctat ccaacggttt
    13201 tgggtttttt cgatggggtt gtcgggcatg gcgaagggga gtttggcgat gatgacttgc
    13261 acgcaggcgg tgccgggcag gtcgagccct tcggcgaagc tgtcgagtcc gaagatgatg
    13321 ctggctttgc cttcttctat ggcttggtgg tgtttttgca ggaggacggc tttgggtaat
    13381 tcgccttgta cgagcaagag cggcaggtag tctccgggca ggtgcagggc gacatcctgc
    13441 atttgtttgc gcgaggaaaa caggacgagt gtgccgatgg cttcggtggg cgaaatcagc
    13501 ttgggcagcc attcgatgac ggcggcggtg tgggcttcgg ggtctttggg gctggcgtat
    13561 atggagggga tgtagagttc gccctgtttt tcaaagtcaa aggggctttt gagggcgagg
    13621 gtggtggttt cgggcagcca cagcaggccg gtttggcgca aaatcaagtt gaagctgccg
    13681 agcgattgca gggtggccga agtcagcacc gcgcctgccg cgcgccgcca caggctgttg
    13741 gcaaggtggg acgcgctgct gatggggctg gcattgaaaa tatagtcgtt tttgtcgtcg
    13801 gcacggcggg ctatccattt cgccaacggc tcctcgccct caatggaaac agtggagagc
    13861 aaatcccaaa ccgcgctgat ttgttcgata cgggcgataa aaaggccgaa ctcgccggtc
    13921 aggcggtcga tgagcgcacc gtcctgttct ttttcgcggc gtgcggcgga aagcgcatcg
    13981 ttcagcccga caacgtgttt gagcaggctg cgtgcagcaa tggccgtatt ggaaacggtg
    14041 gtttcgaggc cttcggggat tttgccgtct tcccacagcc aagtcggttc gctgttggtt
    14101 cgtctgtcgt tttcagacag ccccatactt aaagacggct cttccgccaa atggaattgc
    14161 cattcgtgca ggctgtcgag caaggatgcg gcggcttcgt cggcaaggtt ggcaagttcg
    14221 gctttatcgg taagcgcggc aattttgccg gtaagctgcg gcagtttttc cagcgtccaa
    14281 acggcaatat tccatgaatg ttcggcggca aaacggctga gggctttttt gggcaggtgg
    14341 tgcgcttcgt ctatgcaata gaaactgttt tcgggcgcag gcagaatcac gccgccgccc
    14401 atgctgatgt cggcaagcag aagatcgtgg ttggcaacaa cgacatcgac ggtttccaac
    14461 atatcgcgtg ccaggtaaaa agggcattcc ggacggttgg gacaggcggc tttcaggcag
    14521 ccgtggcggt cgttggtcac tttgagccaa atcgcgtcat cgattttttc cggccaagcg
    14581 tcgcggtcgc cgttgaaccg tcgggcggaa aattcgtcgg cgatgtcgcg cagcagcttc
    14641 aattcttccg gcttgggttt gctgtcccac aagacggcgg gggcttcaaa gccgagcagg
    14701 ttttgctggg cgttgctttg cgtcagttga tagagtttgt aggggcagag atagcggccg
    14761 cgccctttgg caagtgcgaa ggtcagttcc aaaccgcttt tttcgaccag aaacggcagg
    14821 tcgcggtcta ccaactgctc ctgcaaggca accgtcgcgc tgctcacaat cagccgcttg
    14881 ccgcgtgttt gcgccatgat gccgcccgca agcaggtagg caagcgattt gcccacgccg
    14941 gtcggccctt cgattacggc aatgctttcg ccctcgcgct tgggcggctc gccgccttct
    15001 tcgcgcgtca acgtccgcga aaaagcgttg gcaaccgccg caatcatttc ccgctgcgaa
    15061 gcacgcggac ggaaaccggg caggtttttg ccgatatttt ggtaatggtc gcggatggcg
    15121 tttttttcta aatcggtgag catagtatcg gtgggcggat gatttggggt cattttaaca
    15181 ttgcgcggat gcggtacggt atcgttgtcg gacggtggtt cgggcatatc tgattattga
    15241 aagtaaaaaa aacggaagat attgggtcat ccgtacaaaa tgccgtctga agcgggaaac
    15301 gggcttcaga cggcattgcc gcgcttatac gccggcgacg gggcgggcgg gatagccgtg
    15361 ggtatcgacc gaacaggcaa accgccaagg cgtgtggacg gtgtcggcgg acaggtgggc
    15421 gagttcggga atgtgccgtt tgataaaagt accgtccggg tcggtacggt atgaggcggc
    15481 ggcaatgtcg gggcaggtgt gccgtgaggc ggcaagccgc cagttgcctt ggttgattgc
    15541 cgcatcgaaa tcggtcagct gccgggcaaa ccatatctcg ccttcgcggc gggggaggtt
    15601 taaaacgtgg cagaaaaaat ccgcgctcaa gcgtctcagg gcggggtgga ggctgccggt
    15661 tttgtgcaaa cagcgcatcg cggcatcgat aatcggaatg ccggtccggc cctgctgcca
    15721 aagcgtcagg cgcagggtgt gttccggatt gccgtctgaa gggtcgtcat ccgcgtgctg
    15781 caaggcaagt tgaaggaaaa aatcgcggcg gatgatgttg tccgcccacg cgttcagacg
    15841 gcgttcgagg ctttcccgcg cgagcaggcg cggcgagatg cagccgacac tcaaatacgc
    15901 gcccatcagc gaagtgtttt tgcgcgaagg gaaatccttt aaaacggagt aggaagccgc
    15961 ctgttcgaga aaccgccgcc actgccgcca agccgccgtt tcgccgccgt tttgcggcag
    16021 gaagatgccg tctgaaagcg cggcaggcgg cggggcgggc aggttttcgg ggaagggttg
    16081 gcggtatgcc gcgaataggt ccgaaccggc ggggggctgc ttggaaaagc ggtcgagcca
    16141 tacttcgcgg tagcggttga aatcggtgtg tgccgtaccg ttgtctggtg tcaggtcggt
    16201 tttgccgaaa acggaacggt cgttgacgaa ggttaacgcg atgccgtgtt tgtccaattc
    16261 gtgccaaagg gcgttgtcgg cgagtttgtc ggcaaaagta tgggattcgt cggcgatgac
    16321 ggcgcggata ttgaggcgga cggcgagccg gacaagcccg gcaggcgatg ccgccgtgta
    16381 gagcgggatg ccgcgccttg ccagcccttg ggcgagttcg gcggcggatt ggcggtagaa
    16441 cgcggcgcgg cgcgggttgt ctgtttcggc atcgtcaatc caaatgccga taacgggcga
    16501 accttcggca acggcggcgc ataaggcggc gttgtcgcgg atgcggaggt tttggcggaa
    16561 ccagacgagc gtgtgcgcgg cgcgcgtgtc cgcataaagg gggagggcgg tttcagacgg
    16621 catttcggca gcctttcctg ctggcgattt tttcgttcag aaaatcgatg aaactgcgga
    16681 ctttcgcact caaaaatgcc ctgtccgcat aaacggcgtt cagccggtcg gtcgggacgg
    16741 cgtatccggg cagcagcctc accagcgggc cgcagcgcaa atcgtgttcc gccgcccaaa
    16801 gcggctgata accgatgcac gcgcccgcct taatcatttc gcgcatcatc agcgtgttgt
    16861 cggtacggat gacgggggtc agttcaagcc ggtatttctt gccgtccgat ttgcgggtga
    16921 ggtcgagttt ctgctggttg gtgtaggtcg gcaggacggc aggcagcccc gccacttctt
    16981 ccggtgtttc cggcacgccg ttgcgcctca ggaagtcggg cgaggcaagc agggcaaatt
    17041 cgatttccgc cagcgggcgc gcaatcagcg aaggggaaag ggtttgggaa acgcgcaacg
    17101 ccaaatccac gccttcggca atcaaatcga cgtggcggtt gtccaaaatc agttccaacg
    17161 ccacttcggg ataacgttcg cggtattccg ccagccagtt gcatatctgg ctgccggcaa
    17221 accacagcgg catcgttacg cgcagcagcc cctgcggttt ttccgtcccc ccggcggctt
    17281 tttgcgcggc atcgtcgagc gtgtcgagcg cgtaactgca ttgccggtag tattcttccc
    17341 cggcttcggt caggctgagg ttgcggctgt tgtggtgcag gagtttggct tggacggtgt
    17401 tttccaagtg actgacgtgt ttgcttgcca ttgcggtgga gatgccgagc gcgtcggcgg
    17461 cgcgggtgaa gccgccgctt tggacgactt ggcggaaaac tttgaggctg aacagggtgt
    17521 ccatattttc ttgtgtggaa aagttgtatc aataaaagca gtatatattt gaaaaaggga
    17581 aacatctata ctctgccgcc tgaaatgaag acaaatatca aaggagcttt tatgtccgat
    17641 tgctgcaacc gtatccaacc ggttttgctt tctgttttgc gtatcgtaac cgcctacctg
    17701 tttttgttgc acggtacgtc gaaaatcttc gccttcccca ttgaaatggg cagcggttcg
    17761 cccggcgggc tgttgctgct tgccggtatt ttagaaattg tcggcggcat tttgctggtg
    17821 ttgggcctgt ttgcgcgccc tgccgcgttt gttttgtccg gccagatggc ggttgcctat
    17881 tttatggcgc acgcttccgg aaatgctttg ttcccgattg ccaacggcgg cgagtccgca
    17941 gtgctgttct gcttcgtatt tctctatatc gcggcagcgg gcggcggagc atggtcgctg
    18001 gacaggctgt ttttcaagcg taaagcctga accggactgc ctaaagtgta ttttgttgaa
    18061 tgtttttgag gaaaagaaat gagccgtcaa tccctgcaac aggctgccga aagccgccgt
    18121 tccgtttatt cgttaaataa aaacctgccc gttggcaaag atgaaatcgt ccaaatcgtc
    18181 gaacacgccg ttttgcacac acctttttcg ttcaattccc aatctgcccg cgtggtcgtg
    18241 ctgtttggcg aagagcatga c
//
LOCUS       DAWTHD010000034        18240 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWTHD010000034 DAWTHD010000000
VERSION     DAWTHD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18240
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.034"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(161..553)
                     /gene="rpsI"
                     /locus_tag="WM704_000901"
     CDS             complement(161..553)
                     /gene="rpsI"
                     /locus_tag="WM704_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFA9340349.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(566..997)
                     /gene="rplM"
                     /locus_tag="WM704_000902"
     CDS             complement(566..997)
                     /gene="rplM"
                     /locus_tag="WM704_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFA9340350.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1175..1354)
                     /gene="ssrS"
                     /locus_tag="WM704_000903"
     ncRNA           complement(1175..1354)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WM704_000903"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1360..1665)
                     /locus_tag="WM704_000904"
     CDS             complement(1360..1665)
                     /locus_tag="WM704_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFA9340351.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            complement(1676..2005)
                     /locus_tag="WM704_000905"
     CDS             complement(1676..2005)
                     /locus_tag="WM704_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340352.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(2059..3048)
                     /locus_tag="WM704_000906"
     CDS             complement(2059..3048)
                     /locus_tag="WM704_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFA9340353.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3171..5873)
                     /gene="ppc"
                     /locus_tag="WM704_000907"
     CDS             complement(3171..5873)
                     /gene="ppc"
                     /locus_tag="WM704_000907"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFA9340354.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5916..6809
                     /locus_tag="WM704_000908"
     CDS             5916..6809
                     /locus_tag="WM704_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HFA9340355.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPCNPECPVCGAAR"
     gene            complement(7209..7433)
                     /locus_tag="WM704_000909"
     CDS             complement(7209..7433)
                     /locus_tag="WM704_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFA9340356.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7434..8822)
                     /locus_tag="WM704_000910"
     CDS             complement(7434..8822)
                     /locus_tag="WM704_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFA9340357.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(8908..9729)
                     /gene="prmC"
                     /locus_tag="WM704_000911"
     CDS             complement(8908..9729)
                     /gene="prmC"
                     /locus_tag="WM704_000911"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFA9340358.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            complement(10146..11000)
                     /locus_tag="WM704_000912"
     CDS             complement(10146..11000)
                     /locus_tag="WM704_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9340359.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTTADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            complement(11113..11844)
                     /locus_tag="WM704_000913"
     CDS             complement(11113..11844)
                     /locus_tag="WM704_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9340360.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            complement(11846..12514)
                     /locus_tag="WM704_000914"
     CDS             complement(11846..12514)
                     /locus_tag="WM704_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9340361.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            complement(12504..13163)
                     /locus_tag="WM704_000915"
     CDS             complement(12504..13163)
                     /locus_tag="WM704_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9340362.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            complement(13307..14791)
                     /gene="tldD"
                     /locus_tag="WM704_000916"
     CDS             complement(13307..14791)
                     /gene="tldD"
                     /locus_tag="WM704_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFA9340363.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      14866..14970
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15036..16262
                     /gene="cytX"
                     /locus_tag="WM704_000917"
     CDS             15036..16262
                     /gene="cytX"
                     /locus_tag="WM704_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFA9340364.1"
                     /translation="MSGNASSPSSSAAIGLVWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVKLSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAEIPVAVGVTLIGTVLAVMLPVTEYKNFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKAQSLQRNPS"
     gene            16259..17359
                     /locus_tag="WM704_000918"
     CDS             16259..17359
                     /locus_tag="WM704_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFA9340365.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTLTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            17381..17998
                     /gene="thiE"
                     /locus_tag="WM704_000919"
     CDS             17381..17998
                     /gene="thiE"
                     /locus_tag="WM704_000919"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFA9340366.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFPTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         3817 a         5098 c         5164 g         4161 t
ORIGIN      
        1 ctccagtaac agccatccct gacggtcagt tcgtccgtgt agtcgatact ggtgctgttt
       61 ttgatttttg cccaatccgt aatctttaga ttaccaatgg gaaaccgcct gttacaaata
      121 aaaaaccctg cgataagcag ggttttttga atttccaaaa tcaacgtttg gagaattgtt
      181 ttgcacggcg tgctttgcgc agacccggtt ttttacgttc gacttcgcgc gcatcgcggg
      241 taacgaaacc ggcttgagac aaggcaggtt tcaaagcagc gtcaaaatca atcagggcac
      301 gggtaatacc gtggcggatt gcgccggact ggccggtttc gccgccgccg acaacattta
      361 ctttgatgtc gagagattcg gcgttttcag tcagaaccag aggttggcgg acaaccatac
      421 ggctggtttc gcgtgcgaag aattcgtcaa cgggacggcc gtttacgatg atttgacccg
      481 tacctttagt caggaataca cgagccactg aacttttgcg gcggcctgtg ccgtagtagt
      541 atttaccgtt catgtcgtgt ccttattatt tcagttccaa aactttgggt tgttgcgcag
      601 catgggcatg ctccgcacca gcgtacactt tcagtttttt aatcatggcg taacccagcg
      661 gacctttggg cagcatacct tttacagcct gctccaaagc gcggcccggg aattgatctt
      721 gcatttcgcg gaaagtgcgc tcgtagatgc cgcctggaaa accggaatgg cggaagtatt
      781 ttttatcttc gaatttggca ccggttacac gcagtttgtc cgcattgatg acgatgatgt
      841 aatcgccggt atcgacgtgg ggggtgtatt caggtttgtg tttgccacgc agacggctgg
      901 cgacttcggt tgcaacgcga cccaagactt tgtcttgggc atcgatgacg aaccattcgc
      961 gcttcacctc gtggggtttc gctgaaaagg ttttcatagt ggaaatccag atagatatag
     1021 aaagttatgg attttaaaga caggatttga ttttgtcaat cgcattaccg cgttacggaa
     1081 ggattttccg gatttcggca gactgcatac tgctttttcg ggcggggcgg cggccaatgt
     1141 gaaaaaccgc atcgttgcga tgcggttttg aatgggaatc cccgcgagag ccgtttcggc
     1201 cgaatccgct tgaaccttgc tgacaaggcg gctgccccgg gtagtttcgg gtgcgtccgc
     1261 aaaaggacgc tcgcgcccac tactgctccc ggcaacctta agcgaactta ttggttcaaa
     1321 ggaatatatg ccttcgcgga caccgcaggg aaaaaggggt tattcctgcc ccaagcggga
     1381 tagtgccttt tggcaggcgt tgtccatatc ggttatttta cgcgcaaaat cgccgattgc
     1441 caaatcgccg ccgttcaggg aggttttcaa taggtcgtgg acgacgttga gcgcggccat
     1501 aatgacgatt ttttcgctgt ccgcgacgcg gccgccttcg cggatggctt cggctttgcc
     1561 gttgagcatt ccgactgcct gcaacagtgt gtctttttct tctgccggcg tgttgacggt
     1621 cagccgggcg tgcatgactt cgatgtagac ttgttcgatg ttcatccttt aatccttatt
     1681 gctgcgtttc ctgccgttgg gggaggcgcg ctgccagtgc gctgattttc tccctgctct
     1741 gttcgagcag ggcgcggtat cgtgcatttt cttccgtcag gctgtcgatt ttgttttgca
     1801 ggtcttcttt gagtttgccg acttggacga gcagggcttc gctgagttcg tcgacggcgg
     1861 tctcgtgttc gagtttttgc cgctcgtgcg cccgttcgag ttcggcgacg gtttctttga
     1921 ggcggcggtt ttcgctgacg agggtttcga atttttgtac caacgtataa acgctgcttt
     1981 cgagtttttc gatattttgt ttcatgacct tacctgtctg tatgccgtct gggggttcag
     2041 acggcatctg tttgttgttt attcgaaacg cgcgctgcgt tccatcagcc tttcgacgac
     2101 ctgctgaggg gtcatttctt tgcggatgag ttgcagcagg gtttgggtaa tcggcatatc
     2161 gatttggtat ttgcaggcgg tattgaagac ttcttctatg gtgctgaccc cttcggaaac
     2221 gtgcccgatt tcgaccagca cctgatgcag ttccttgcct tctgccaaac ccaagccgac
     2281 gcggcggttg cgcgaaagtg caccggtaca ggtcaagatg agatcgccga tgccggcaag
     2341 ccccatcatg gttttgggct gcgcgcccat tgcggaggca aggcgggtga tttcggccag
     2401 tccgcgcgta accagtgcgg cacgggcgtt gagtccgtat tcgagtccgt cggagagtcc
     2461 ggtagcgatt gccatgacgt ttttgactga accgcctact gccacgccga taacgtcggt
     2521 actgccgtaa agcctcatga cggtcgtgtt gagctgcggt acgagttctt caatccactc
     2581 ttggttttcg gaggcgagga cgacggcgca gggcagttgt ttggcgagtt cctgtgcaaa
     2641 actcggaccg gaaagtacgc cgattttctt attgtcgggc aatacttctt tcaagacttg
     2701 gaaggtcagc agccctgtat cctgctcgaa gcctttgcag gcggcgagga cggggaggtg
     2761 tcccgcgccg tactgtttga gcagctctgc gctgcttctc aatccggcaa cggaggttac
     2821 gataaggaca agtccgctgt ctttgagcgc ctctgccaaa tccgcacaca cttccaaggt
     2881 ttcgggaaag gaaaagccgg gcagtccgcg tttgttttca cgcgcttcct gcatttggcg
     2941 gacttggtct gcgttgcgcg tccacaggga tacgcggttg ccgtgttggg aaaaatgcag
     3001 ggcgagcgcc gtaccccacg aacctgcgcc gataacggta attttcattg gtcgtctttc
     3061 aacatatcac tgccgttcac tttaaaacaa tcggtgtttc tctgcaagtg cggtcaggta
     3121 aatgccgtct gaaaggcgtt cagacggcat tttgccccga tgcggcacta tcagccggta
     3181 ttgcgcaaac cttgcgccac gccgttgatg gtcaggtgca ccatcagcag ggcgtgcgga
     3241 ttgtcgggtt ctttgcgcag gcgtttgagc atggcgactt gcaaaccgtt gagcgcgttc
     3301 aggtatggaa tcctcaaagc gagcgaacgg gcgaggctgc ggttgtcgcg caaaagttct
     3361 tcggtttgca gcaggtcgag cagggctttg cggctgcgtc ggtattcttc cttaatcatg
     3421 ccgaagatga cttttgcctt ttcgggtgat tcgctcaaac cggcgtagtt ttccgccaag
     3481 gtgatgtcgg ttttcgccat cacttgttcc atattggaga gcatcgcttg gaagaagggg
     3541 ttgttttggg cgtgtccgcg cagggcggcg agggtttcgg ggctgccttc gcacaaggtt
     3601 tccaccgcgc tgccgaaacc gtaccaagcc ggcagcatga ggcggttttg catccaggag
     3661 aatacccacg gaatcgcgcg taaatcctga attcgcgcca gggttttgcg gctggcggga
     3721 cggctgccga ggttgagggt ggcgatttcc tgaatcgggc tggtttgcag gaagtagtcg
     3781 atgaagtcgg ggtgggtaat cagttcgcgg tagtatttga acgatacgtc cgacaatgcc
     3841 tgcatcagtt tggcatcagg gtctttttta tccggcagga tgctggcttc caaagtcgcg
     3901 gcaaccaagg tttccaagtt gcgttgggcg ttgccggggt cggcgtattt ggcggtgatg
     3961 acttcgcctt gctcggtaat gcggatttgt ccggcaacgc tgcctgcggg ttgcgccaag
     4021 atggcttggt aagacgggcc gccgccgcgt ccgacgctgc cgcctcgacc gtggaacaag
     4081 cgcatacgga catcgtattt tttgaagagt tcgaccaagc ccaattccgc ctgatgcaga
     4141 caccatgagc tggtaacgta gccgccgtct ttgttggagt cggaatagcc gagcatgatt
     4201 tcttggatgt tgccgcggct ttcgagcagg gcatcatacc agtcgaggcg gaacatggtt
     4261 tccatgaccg gacaggcatt ttcgagcgct tcgatggttt caaacagagg cacgatgttg
     4321 atgcggctgt gcggtttgcc gttttccacc gccaacaggc cgctttcttt cagcagcaat
     4381 gccaaggcga gcaggtcgcc gggttgttcg cagttggaaa taatgctttg tgttacggca
     4441 tcttcgccaa attcgtcttt gattttgcgc gcttcgttga aaatcgccaa ttcgcggcgg
     4501 gtatggtcgc tgtatgtgat gaacgggctg tacagcggac gttggtggcc caattcgcgc
     4561 aacaaggcgg cttgtttttg ctcttcgttc aggctgttgt agtcttccaa gcctgcgtgt
     4621 tggaaaagct cggcaaccac atcggcgtgt ttgcctgcgt gttggcgcag gtcgagcggc
     4681 atcatgtgga agccgaacac ggacacggaa cggatgaggt ctgccaaacg gccttcggca
     4741 agcaggcggc tgccgttgtc gataagggaa cgttgcaatt ttttcaaatc atcgagaaat
     4801 ttttgggccg aagcataagg ctcgagaaag ccgaatttgc agcccatgcc caaaccgagc
     4861 gagcgcgctt tgcccatagc gcgcgccata atgtaggcaa tggcgcggcg gtaaggttct
     4921 tcggtgcggg cgatttcttc gtcaggcgag agggctgcca gtgccattac gtcgtcgttg
     4981 actttgacgc ggcggatgga aagcggcagt tcgcggtaaa gtttgtcgag ttcgctgcgg
     5041 taaaaacgga acacggcatc ggcgtggcgg cggaaggcaa agcgcagggt ttcgccagaa
     5101 acaaacggat tgccgtcgcg gtcgccgccg atccagccgc cgattttaag gatgttcgga
     5161 acgcggacat cgggataggc cgtctgaaag tcgtgttcca tcttgcggta gagtttgggc
     5221 agggcttcaa aaaagctcat cgggaagatg gacacgccgt tgttgatttc gtcgttgacg
     5281 ctgagtttgt ggcggcgcgt ttcgctggtc tgccacaagc ccagaagcac ggtgtcgatt
     5341 tcgcggcgca gccgtgccag cgcgtcggca ttggtgcagc gttcgcgttg cggcagcagc
     5401 gcgcggatgc ggcggttgaa attcaaaacg gtttggcgtt gcacttcggt cgggtgcgcg
     5461 gtcaaaacgg cggtaacgga cgtattgtcc aactgccgct gcaccgattt gccgtcggct
     5521 ttccccgctt tgagcctgcg gacggtttcc gtcaggctgc cttctgctgc gttgtggccg
     5581 gcatcttcgt ggatttggcg gcggcgttcg tggtgcacgt cttcggcgat attcagaatc
     5641 tgggcgaaca gcccgcaggc aagcgtcaga tcgtaggtct gccgttcgtc caattgcggc
     5701 aatacttttt caatcaatgc cgcgctgtcg tcggaagtgg acaagagttt gaccgtttcg
     5761 acaaccaacg gcgaggcttc ttcgtgcagg aggttgaaca gggactgttt caaaaattcc
     5821 gcgtccgccg ccaaagccgc gtccttcgga ttgttcagga tatgcagttg catgattttc
     5881 ctctcattgc cgtaaatact gtaaatgtac ctcaaatgcc gcatccgtgc caaaccgttc
     5941 acactttaac cactcatgtc ccgaaatgcc gtctgaagtt gaacgccgcc cgacggcggc
     6001 gttacaatcg cccgcaactg ttttttccga acatcatcat gaccgcgacc gaacacgaca
     6061 acgacgacgc actcctgctg cggtacagcc gccacatcct cttggacgaa atcggcatcg
     6121 aagggcagca gaagctttcc gccgcgcata ttttggtcgt cggctgcggc ggattgggcg
     6181 ccgccgccct gccctatctc gccgcctcgg gcgtcggcac gctgaccata gccgattccg
     6241 acacggtcga gctgcacaac ctgcaacgcc aagtcgcatt tgacgagggc gatgtcggca
     6301 agcctaaagc cgaaaccttg gcaggccgcc tgaaacgcat caaccatact gtcgatgtcc
     6361 gcgccgtcaa cgaaaaactc gacggctgcc gcctgaccgg tttggttcaa accgccgaca
     6421 tcgttttaga ctgctgcgac aactacgcca cgcggcaggc ggtcaaccgt gcctgcgtgc
     6481 aaacgaaaac accgctggtt tcaggggcgg cggtacgttt tgaaggacag cttgccgcat
     6541 accgtcccga cttgcccgac tcgccgtgtt acgcctgcct gttcgacggc ggatcggctt
     6601 cagacggcat ctgttccctc ttcggcgtgc tctcgccgct ggtcggcatc gtcggctgca
     6661 cacaggcggc ggaggctctg aaaatcctgc tggaagcggg cgaaccgtcg cacggcaggc
     6721 tggcggttta ccgtgccttg gaagggggct ggcaatattt cgacctgccg tgcaaccccg
     6781 aatgcccggt ttgcggcgcg gcacggtaaa accctgccgc cgtttcagac ggcatccgaa
     6841 cggatgcggc ggaacggttt taaaaattta aaaatttaca tttctttgca aaaaaataaa
     6901 cttaccttat aattgcagtt gttttagcaa tgtctgtttc gcagactcat tgagtaagac
     6961 gttttccccg taatgtgttt ggccgtctgt cccctttggg ttcggacggc tttttttggc
     7021 tgtgtttgaa tacccggttg gttttatctg tttgcagcgg gggaagccgc tcatcgccgt
     7081 tcggacaaga accggtttca tcggataaaa ggcattttgt ccgactgatt aaggttacat
     7141 cttagggttg caccggcgga atattcaaac acagccttta tttaaggaaa tccggatacg
     7201 gcggcgcatc aataatgcgg cggaatctcg tcgcgcaggg aatacggctc ttgcgcgtcg
     7261 ggattcctgt cctgcatttt ttgatacagc agcctcaact gagcctgctg caaatccaac
     7321 gtctgccgca attccgccac catcgcgttc agaccggaga ttacgtcctc ctgaagcgcg
     7381 gtttggattt ccagttcgac aatacggcgt tccaactctt gaaccgcgtc catttacagc
     7441 accatcgcgg caatccagcc ggcaatcaga agcgggatgt tgtagtggat gaaggtcggg
     7501 atgacggaat cgcggatatg gtcgtgccgc ccgtcggcgt tcagccccat cgtcgggccc
     7561 agcgtggaat cggacgcggg cgaaccggca tcgcccaacg cccccgccgt gccgacaatg
     7621 gcggcggtgg caagcggcga aaagcccaaa ccggtacaca gcggcacata aatcgcggca
     7681 ataatcggca aagtggaaaa ggacgaaccg atgcccatcg ttaccaaaag ccccaccacc
     7741 agcatcgcca acgccgccat acctttgctg ttgccgaata tcgccatact gctttccacc
     7801 agcggctgaa tatgcccggt cgcattcatc acggcggcaa aaccctgcgc ggcaatcata
     7861 atgaagccga ccatcgccat catcttgatg ccttcgccga atacgtcgtt tgccttgtcg
     7921 cggttaatga cccccaacat cataaatacg gcgaaaccga gcatcgcgcc caacaccagc
     7981 gaatcttcat acatcaactg tatggcaaag catacggcga tggcggcggc ggcaaccagg
     8041 ctgcggtagg cggacggctg cggacggttt gccgcatcgg cgttgcccgc cgtatcggca
     8101 ttgttgcttt ggtacagtcg cggtttgcgg tagtggacaa acgccagcag gagtccggcc
     8161 agcattccca atgcgggaat cgccattgcc gccatcacgt tgatgttttt cacatcaagc
     8221 tgcggcgcgg cggaatggat gttgcccaac aggatttcgt tcaaaaaaat cgcgccgaag
     8281 ccgtaaggca ggaacatata agtcgtaacc agcccgaaag tgatgacgca cgcaatcagg
     8341 cggcggtcga ttttcaggcg gttaaacacc aaaagcagcg gcggaacaat catcgggata
     8401 aaggcgatgt ggatgggtat gacgttctga ctcataatgc ccatcacaag gatgatggaa
     8461 agcagcagcc atttgaccgc gccctcgccc gaacgcacgc tgtcgggcat accgccccgg
     8521 ttcagcttgc ggacgaccgc gccggcaagc tgctgcggca ggccggaatg ggtaatcgcc
     8581 atcgcaaacg caccaagcat cgcataagaa agcgcaatct tcgcgccgcc ttccaaacct
     8641 ttgttgaaca cggggataat ccccgcctga ctgacctgtc ccgccgcatc ggcaatgttt
     8701 tgcagcggca tacccgccac cgcgccgccg acaaacgcgc cgaccgtcag gctcaatacc
     8761 acgtgcacgc gcgacagcga cagcaccagc ataacgatta cggcaactac gactgcattc
     8821 attgtttatc gctccaaacc tataaatgtt tacatatcaa aacacatcat aacccaataa
     8881 cggaaaatcc gccaattttg caaacaatta tttcaaatgc ttcatatact tccccagcgt
     8941 aaccctgtcc aaaccggcca aatccggcag gatttccact cccgaaaaac cattctccgc
     9001 caacacgccg cgcaccgccg cgccctgatc gaaaccgtgt tccaacaaca aaaaaccgcc
     9061 ttccgccaaa cggtcgggcg cgccttgcgc caaggtgcgg atgcagctta ggccgtctga
     9121 aaagtcggtc agcgcgattt gcggctcaaa ccgcaaatcg ccttgcgaca aatgtttatc
     9181 gccgttttcg atatagggcg ggttggacac gatgatgtcc cattgccttt cagacggcat
     9241 atcggtgtcg aaccacgaac cgtgtgcaaa ttcgacccgc gcgcccaaat ccgccgcatt
     9301 tttccgcgcc gtttcaaggg cgggcgtgct gatgtcggat gcgcgcacaa acgcatcggg
     9361 gcgttcgagc gcgacggtta cggcaactgc gccgctgccc gtccccaaat cccacacgcg
     9421 cccgttttcg ggcaggcgcg ccaatacggc ttcgaccaag tgttcggttt cggggcgcgg
     9481 aatcagcacg ttcgggttga ctgtaaagcg tctgccataa aattcgcgca cacctaaaat
     9541 ataggcaacc ggctcgccgt tcagacggcg ttgcgccagc ctgtccgccc gctgtcggat
     9601 ttcgtccggc atttcttccc cgccccgcgt caacaactgc acgcgcgtat attccgaaac
     9661 atattgcagc agcatccttg cttcaatttt aggcagtttt gacaagccca accattcgtc
     9721 aaacgtcatt tttcgtcccg tctgccgccg atgcggcttg aacatttttt ccggctttgc
     9781 ggcacgcttg ttcaaaaaat atcattttgc attgaaatta tataataaag gaaaacacct
     9841 tatcgccgtt tcaataccgt aggaatttat ccgaataaca gaaacgctcc gccgtcatcc
     9901 cgcgaaaacg gtcatccgga aacataaggc tgaagcgttt tatctggaat gacgatttgg
     9961 aagtttcccg aaattcaaaa acaggcaaaa ccggacgaac ttgatttccg cacgcgcaag
    10021 aataataaaa taaataaaac gatgagatag ttgatttttc ctttgaattt gcaatatttt
    10081 cggcggctga aaaaaaatcc gcacccgcct tcaaatgcgg atacggattt tcttgtcaaa
    10141 caaacttatt cggccaacaa tgcttccggt ttgactttct cgccatacac gggcaagagg
    10201 gttttttcat aggcggcttt cagacggccg tcttttttca tggcggcgat ttcgccgttg
    10261 acccagttca ggaggtcggc gttgcctttt tgaacggcgg gggcgataaa ttcggcagga
    10321 ccgagattgc cgatggcgac ttcaaagttc gggttttctt tcgcccacgc ccacagcagg
    10381 gcgttgtcgt gcgcgagtgc tacgccgcga ccgtctttca gcgcgtcgaa ggtttcagtg
    10441 ttttggtcga atttcaacag cttgacttcg ggatggcttt tggtgaagaa agcgtcggcg
    10501 gtggtgcctt tgttgaccag cagggtttgg tctttcaatt gcgccatgtc ggtaatcggt
    10561 ttgtttttgg gggaaaccac gcccaacgcc actttcatgt aaggatcggc gaaatcgacg
    10621 gcttcggcgc gttcgggggt ttgggtaaag ttggcgagga tgaggtcgac tttgccggaa
    10681 cggacgtatt cgacgcggtt tgcggcctcg gtcaggacga attcgacttt gtcggggctg
    10741 ccgagcaggt ctttggccag gtctttggcg atttcaacgt caaagccttg gtttttgccg
    10801 tttgcgtcaa catagccgaa cgggggtttg tcgccgaata cgccgatgcg gataacgcct
    10861 ttttctttga tggcggcaac ggttgccgca ccgctgcttt gtgaagattg ggcatcgccg
    10921 gagccgcccc cgcaggcggt cagaccgacg gcgatggcgg cggaagccaa aagggctttg
    10981 agtttggcgt tcaatttcat tggaaatctc ctttttgaat gtttgcttgg gatgtttcaa
    11041 cacacaggac gacacataaa gcactgccct atgaaatgcc gtctgaaaag ggttcggacg
    11101 gtttgcggta aatcagtagt ccatacctgc cagaaattgg cgggcgcgtt cgctttttgg
    11161 tgcggaaaaa aaggtttcgg ggtcggacga ttcgacgatg ccgcctttgt ccataaagac
    11221 gatgcggtcg gcaactttgc gtgcgaaccc catttcgtgg gttacgatga gcatactcat
    11281 cccttcgcgg gcgagttcca aaaccacttc caagacttcg cgcaccattt cggggtcaag
    11341 tgcggcggtg atttcgtcca gcaggatgac ttccggattc aggcacaggg cgcggacaat
    11401 ggcgatgcgc tgtttctgac cgccggaaag ttcgcgcgga taggcgtttt tgcggtctag
    11461 cagtccgacg cgttccaaca gtttgccggc ttgcgcctct gcttcggcac ggtcgcggtt
    11521 ttgtaccttt accgggccta agaggatgtt ttcgatgacg gtcatgtggg caaacagttc
    11581 gtagctttga aagaccatac cgactttttg ccgggcggtt tgccaggaaa cgtctttgcc
    11641 gaattcgccg acaccgtcca tcacgatgct gccgccttgg tgcggctcca aaccgttgac
    11701 gcagcgcagg agggtggatt tgccgcagcc ggacgggccc agcagtacga tgacttcgcc
    11761 tttttccaag tccaagtcta aggattggat ggcggttacg ctgccgtatt gtttgtgcag
    11821 cttgcggatg ctcagtaaag ccatgtcagt gttcccattt ttgttcaagt tttgccgcca
    11881 gtagagatag cggccagcaa cagaagaaat acagcataaa aatcaggccg taaacccaaa
    11941 atgaagcatt gggctgcgtc agcaacgagt tttcaataat ttgctgcccg actttgacga
    12001 cttcgatcac gccgataagc caggcgagcg agctggtttt gatcatgcgc gtgaacaggt
    12061 tgaccgcgcc gggcaatacg cggcggatgc tttgcggcag ctcgatgcag cggaatacct
    12121 gcccccggct caagccgggc gcaagacccg attcgacctg gtgtttttca atcgattcca
    12181 gtgcgccgcg caccaagtcg cccatttcgg caacgcccca cagggaaaat acccagacgc
    12241 agacccaaaa tccgccgatg tggatgcctg tccagacgga taggccgaaa tacaggccga
    12301 acagccacac caaaatcggc acgattcgga tggtttcgag ataaaaccgt ccgacaaagc
    12361 ggacaagccg gttgcgcgaa cgcaaaacca agccgaacag cgtgcccaat acgcaagaag
    12421 ccgcaacaga gattaaagaa atttgtgccg ttaagagcag gccttcgccc aaccgtgccg
    12481 cattttttcc ttcaaacagc cattcaaacg ccatatttcg cactccgtac gcggttttca
    12541 atccgccgcg ccaacaaaga aacaggcagc aggataatca aataagcggc aaacagcagg
    12601 aacaaggctt cattggtttt gtagtccata ccgatgacgt ctttggtaac aaataacaat
    12661 tccgcaatgc cgaccgtgct gacgacggat gtttctttca ttaaaaacaa tatatttgcg
    12721 ccgatggcgg gaacggcgac cgcccaaacc tgcggcaatt cgacatagcg gaacacttga
    12781 aagcggctca aaccgattgc cttgcccgct ccgacctgcc ctttggggac ggcgaggatg
    12841 ccggcgcgga cagcttccgc catatagctt gcgcccaaga aaaccagcgc gattacgccg
    12901 caggtgaaac cgtcccattt gatgcccatt ttcggcagac cgtaatatag gaaaaacaat
    12961 tggatcagca ggggcgtatt gcgcgacagc tcgatatagg cgcgcgccaa tgcgtagaag
    13021 gggcggatgc ggtatgccgt taccacggcg acgggcaggc cgaacagcaa cgacaaaaca
    13081 acgccgtaaa cggacaattc cagcgtcagc tttgccgcat cggcgaattt gggtacggca
    13141 tcgattaaat aaggccagtt catcagaaat cagcttaacg aaaggcggta ttttaatgaa
    13201 cttgtaagat aatgttaaag aatgcttagg aatatcaaac atcggaaaaa agaataaggc
    13261 gatgccgtct gaaaaccttt cagacggcat cgtgccggat tccgcgtcag attgcgctgc
    13321 cgccgacggt cagcccggca tcgatacgca gggtcggctg ccccacgccg acggggacgc
    13381 tctgcccttc tttgccgcac acgccgacac cgctgtcgag cgcggtatcg ttgcctatca
    13441 tcgaaacgtg tttcagcact tcggggccgt tgccgataat ggtcgcgcct ttgacggggt
    13501 attgcagcct gccgccttcc acccaccacg cttcggacgc gccgaagaca aatttcccgc
    13561 tggtgatgtc cacctgtccg ccgccgaagt tgacggcgta aatgcccttg tcgatggacg
    13621 cgatgatttc ttccggctcg tagctgccgt tttccataaa ggtgttggtc atacgcggca
    13681 tcgggacgga ggcgtagctt tggcggcggc cgttgccggt cgattgcgta cccgtcaggc
    13741 gggcgttggt ttcgtcctgc atatagccga ccaagatgcc gtcttcaatc aatacggtgc
    13801 ggcgtgtttc gttgccttcg tcgtcgatgt tgagcgaacc gcgccggccg gcaatatcgc
    13861 cctgatcgac gacggtaacg cctttggcgg cgacgcgttc gccgatccgg ccggaaaaga
    13921 cgctggtccc cttgcggttg aaatcgcctt ccaaaccgtg tccgaccgct tcgtgcagca
    13981 acacgcccgg ccaaccgttg cccaaaacga cggtcatctc gccggcaggc gcaggacggg
    14041 attcgagatt ggtcagggct tgtttgacgg cggcatcgac aaactgccgc accaaggttt
    14101 catcgaaata agccaagtcg tagcgtcctc cgccgcccgc gccgccctgt tcgcggcgtt
    14161 cgccctgttt ggcgatgacg gtaacgttca ggcgcaccat cgggcggatg tcggcggcgt
    14221 gtttgccgtc cagacgggcg aggtaaacca tatcgtattc gcaggtcaaa ccggccatca
    14281 cttgcacgat gcgcggatcg gcggctttgg cgattgcttc cactttgttc aacagcgcga
    14341 ctttggcggc ggaatcgagg ccggcaatgg ggtcggatgc ggaacaaacc ggcttgccgc
    14401 gcgtttcaga cggcattttg gcggacacct tgccgcctgc cgccccaatc gcgcggacgg
    14461 cgcgggcgga acggtttatc gaatcgatgc acaggctgtc ggcgtaggca aaggcggttt
    14521 tgtcgcccga aacggcgcgc acgcccacac cctgatcgat ttggaagctg cccgatttga
    14581 cgatgccctc ttccaaatgc cagctttcat aagcggtgcg ctggcagtag atatcggcat
    14641 aatcgacgtg gtgcgcgccg atgatgcaca ggcttttggc gagcagttcg ggggaaaggc
    14701 ggttggcttc gagcagccgc gcctgtacgg cggaataggt cggatgcata gtgtcggcgc
    14761 ataaaaaatc aggggcttga ttatacggca tttgttatgg aaatgcgccg tgccgtcgaa
    14821 aatgtaagaa ttttgccaac gcacgttgct ttgtgtaaac ttaaagcctt cttgtcggag
    14881 tgccgcccgc cgggcggctg agattgcgaa agcaaaatcc gtagaacctg tcggggtaat
    14941 gcctgcgtag gaaacaaaac ctccccgccc tttcgggctt ccgttccctt ttccgcactt
    15001 ccccgaccac cttccttctt tttaaaggac gtcatatgtc gggcaatgcc tcctctcctt
    15061 catcttccgc cgccatcggg ctggtttggt tcggcgcggc ggtatcgatt gccgaaatca
    15121 gcacgggtac gctgctcgcc cccttgggct ggcagcgcgg tctggcggcc ctgcttttgg
    15181 gtcatgccgt cggcggcgcg ctgttttttg cggcagcgta tatcggcgca ctgaccggac
    15241 gcagctcgat ggaaagtgtg cgcctgtcgt tcggcaaatg cggttcagtg ctgttttccg
    15301 tggcgaatat gctgcaactg gccggctgga cggcggtgat gatttacgtc ggcgcaacgg
    15361 tcagctccgc tttgggcaaa gtgttgtggg acggcgaatc ctttgtctgg tgggcattgg
    15421 caaacggcgc actgatcgtg ctgtggctgg ttttcggcgc acgcagaacg ggcgggctga
    15481 aaaccgtttc gatgctgctg atgctgcttg ccgtgttgtg gttgagcgtc gaagtgttcg
    15541 cttcgtccgg cacaaacgcc gcgcccgccg tttcagacgg catgaccttc ggaacggcag
    15601 tcaaactgtc cgccgtcatg ccgctttcct ggctgccgct ggccgccgac tacacgcgcc
    15661 aagcacgccg cccgtttgcg gcaaccctga cggcaacgct cgcctatacg ctgacgggct
    15721 gctggatgta tgccttgggt ttggcggcgg ctctgtttac cggagaaacc gacgtggcga
    15781 aaatcctgtt gggcgcgggc ttgggcataa cgggcattct ggcagtcgtc ctctccaccg
    15841 ttaccacaac gtttctcgat acctattccg ccggcgcgag tgcgaacaac atttccgcgc
    15901 gttttgcgga aatacccgtc gctgtcggcg ttaccctgat cggcacggtg cttgccgtca
    15961 tgctgcccgt taccgaatat aaaaacttcc tgctgcttat cggctcggta tttgcgccga
    16021 tggcggcggt tttgattgcc gactttttcg tcttaaaacg gcgtgaggag attgaaggct
    16081 ttgactttgc cggactggtt ctgtggctgg caggcttcat cctctaccgc ttcctgctct
    16141 cgtccggttg ggaaagcagc atcggtctga ccgcccccgt aatgtctgcc gttgccattg
    16201 ccaccgtatc ggtacgcctt ttctttaaaa aagcccaatc tttacaaagg aacccgtcat
    16261 gacccgtatc gccgtcctcg gaggcggcct ttccggaagg ctgaccgcat tgcagcttgc
    16321 agaacaaggt tatcagattg aacttttcga caaaggcacc cgccaaggcg aacacgccgc
    16381 cgcctatgtt gccgccgcga tgctcgcgcc tgcggcggaa gcggtcgagg caacgcccga
    16441 agtcatcagg ctgggcaggc agagcattcc gctttggcgc ggcatccgat gccgtctgaa
    16501 cacgctcacg atgatgcagg aaaacggcag cctgattgtg tggcacggac aggacaagcc
    16561 attatccagc gagttcgtcc gccatctcaa acgcggcggc gtagcggatg acgaaatcgt
    16621 ccgttggcgc gccgatgaaa tcgccgaacg cgaaccgcaa ctcggcggac gtttttcaga
    16681 cggcatctac ctgccgaccg aaggccagct cgacgggcgg caaatattgt ctgcacttgc
    16741 cgacgctttg gacgaactga acgtcccttg ccattgggaa cacgaatgcg ccccccaaga
    16801 cctgcaagcc caatacgact gggtaatcga ctgccggggc tacggcgcga aaaccgcgtg
    16861 gaaccaatcc cccgagcaca ccagcacctt gcgcggcata cgcggcgaag tggcgcgggt
    16921 ttacacgccc gaaatcacgc tcaaccgccc cgtgcgcctg ctgcacccgc gctatccgct
    16981 ctacatcgcc ccgaaagaaa accacgtctt cgtcatcggc gcgacccaaa tcgaaagcga
    17041 aagccaagcc cccgccagcg tacgttccgg gctggaactc ttatccgcgc tctatgccgt
    17101 ccaccccgcc ttcggcgaag ccgacatcct cgaaatcgcc gccggcctgc gccccacgct
    17161 caaccaccac aaccccgaaa tccgctacag ccgcgaacgc cgcctcatcg aaatcaacgg
    17221 ccttttccgg cacggcttta tgatttcccc cgccgtaacc gccgccgccg tcagattggc
    17281 agtggcactg tttgacggaa aagacgcgcc cgaacgtgat gaagaaagcg gtttggcgta
    17341 tatcggaaga caagattaaa gccgcccgaa aggacaccct atgaccttcc cgcccctaaa
    17401 atccctactt aaattctacg ccgtcgttcc caccgccgat tgggtggggc gcatggtcaa
    17461 agcaggtgcc gacacggtgc aactgcgctg taaaaccttg cacggcaacg aattgaaacg
    17521 cgaaatcgcc cgctgcgtcg ccgcctgtca aggcagccgc acacagcttt tcatcaacga
    17581 ccactggcgc gaggcaatcg aagcgggcgc atacggcgta catctcgggc aggaagacat
    17641 ggacaccgcc gaccttgccg ccatcgccgc cgccggtttg cgtttgggtt tgagtacgca
    17701 ctccgtcgcc gaactcgacc gcgccctgtc cgtacacccc ggctacatcg ccagcggcgc
    17761 gattttcccg accacgacca aacaaatgcc caccgccccg caaggcttgg acaaattgcg
    17821 cgaatatgtg gaacaggctc gcggcacgcc cgtcgtcgcc atcggcggca tcgatttgaa
    17881 caacgcccga gccgtgctgg ccaccggagt ttcctcactc gccgccgtcc gcgccgtaac
    17941 cgaagcggca aatcccgaag cggtggttaa agcgtttcag gctttgtggg atggataaaa
    18001 ccgaaagaag aaaattcaat tgccgtgtag gcaaaactta gcccgttatc gcaaatatgc
    18061 ttaactttaa atgtggcata tcatcaaatt ccgtcattcc cgctcaggcg ggaatccgcc
    18121 gtaaaacttg agaaaccatc atttgaaaaa cagtttccga atttcaaaaa tggattcccg
    18181 cctgcgcggg aatgacggca accggccggt tgcgtatcaa aaaataaagt aattcggcta
//
LOCUS       DAWTHD010000035        18065 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWTHD010000035 DAWTHD010000000
VERSION     DAWTHD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18065
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.035"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..1257
                     /locus_tag="WM704_000920"
     CDS             <1..1257
                     /locus_tag="WM704_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFA9340367.1"
                     /translation="PKKYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASA
                     EGSVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVVVGSEAKVNAERGIAIGKEAKA
                     GGKTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGD
                     FSTAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHS
                     VSLGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVND
                     FDAVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFG
                     TYKGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKV
                     LMEQLMQDNRQLKARLEKLEARLNAM"
     gene            complement(1396..1911)
                     /gene="coaD"
                     /locus_tag="WM704_000921"
     CDS             complement(1396..1911)
                     /gene="coaD"
                     /locus_tag="WM704_000921"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFA9340368.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2041..2724)
                     /locus_tag="WM704_000922"
     CDS             complement(2041..2724)
                     /locus_tag="WM704_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFA9340369.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2881..3147)
                     /locus_tag="WM704_000923"
     CDS             complement(2881..3147)
                     /locus_tag="WM704_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFA9340370.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3221..3691)
                     /gene="rlmH"
                     /locus_tag="WM704_000924"
     CDS             complement(3221..3691)
                     /gene="rlmH"
                     /locus_tag="WM704_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFA9340371.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3744..4130)
                     /gene="rsfS"
                     /locus_tag="WM704_000925"
     CDS             complement(3744..4130)
                     /gene="rsfS"
                     /locus_tag="WM704_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFA9340372.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4189..4794)
                     /gene="nadD"
                     /locus_tag="WM704_000926"
     CDS             complement(4189..4794)
                     /gene="nadD"
                     /locus_tag="WM704_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HFA9340373.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPAAARYIRKHGLYEK"
     gene            complement(4791..5390)
                     /locus_tag="WM704_000927"
     CDS             complement(4791..5390)
                     /locus_tag="WM704_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFA9340374.1"
                     /translation="MSELSEILSYNQKFVESGGYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5419..6960)
                     /locus_tag="WM704_000928"
     CDS             complement(5419..6960)
                     /locus_tag="WM704_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HFA9340375.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7351..7860
                     /locus_tag="WM704_000929"
                     /pseudo
     CDS             7351..7860
                     /locus_tag="WM704_000929"
                     /inference="COORDINATES: protein motif:HMM:NF014500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            7835..8737
                     /locus_tag="WM704_000930"
     CDS             7835..8737
                     /locus_tag="WM704_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFA9340376.1"
                     /translation="MHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYML
                     GKVLGRAIHVPVPELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISE
                     KLVSADETWVQTAKMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPVCS
                     VILITGAGGMFGGVLRASGIGKALADSMADLGIPVLLGCFLAALALRIAQGSATVALT
                     TAAALMAPAVAAAGFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLK
                     TWTANQTLIALIGFALSALLFAIV"
     gene            8757..9275
                     /locus_tag="WM704_000931"
     CDS             8757..9275
                     /locus_tag="WM704_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFA9340377.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9461..10378)
                     /gene="thrB"
                     /locus_tag="WM704_000932"
     CDS             complement(9461..10378)
                     /gene="thrB"
                     /locus_tag="WM704_000932"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFA9340378.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10415..11143)
                     /gene="ubiG"
                     /locus_tag="WM704_000933"
     CDS             complement(10415..11143)
                     /gene="ubiG"
                     /locus_tag="WM704_000933"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFA9340379.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECVRVEDLAGAEPHSFDAVTCMEMMEHVPDPAAIVRACAKLVRPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFISPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11295..12536
                     /locus_tag="WM704_000934"
     CDS             11295..12536
                     /locus_tag="WM704_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFA9340380.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12688..13742
                     /locus_tag="WM704_000935"
                     /pseudo
     CDS             12688..13742
                     /locus_tag="WM704_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(13809..15461)
                     /locus_tag="WM704_000936"
     CDS             complement(13809..15461)
                     /locus_tag="WM704_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFA9340381.1"
                     /translation="MVAYAFLFLFVTAAVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15604..16167
                     /gene="gmhB"
                     /locus_tag="WM704_000937"
     CDS             15604..16167
                     /gene="gmhB"
                     /locus_tag="WM704_000937"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFA9340382.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16197..16940
                     /locus_tag="WM704_000938"
     CDS             16197..16940
                     /locus_tag="WM704_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFA9340383.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16937..17626
                     /locus_tag="WM704_000939"
     CDS             16937..17626
                     /locus_tag="WM704_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFA9340384.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAAASLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWI
                     KIHGRELFALG"
     gene            17708..17839
                     /locus_tag="WM704_000940"
     CDS             17708..17839
                     /locus_tag="WM704_000940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFA9340385.1"
                     /translation="MPPVRGRMAASAPSAPFHFTPPFRNPPDPTCGMKRLSRREVFY"
BASE COUNT         4300 a         5462 c         4595 g         3708 t
ORIGIN      
        1 cccaagaaat atgcagtagc attaggatct agttctactg ccagtggaga gtattcctat
       61 gcgtcgggct ataattctgt tgccagtgga aataaatcct atgctgcagg ctatgcttct
      121 gttgccagtg cagaaggcag tgttgtaatt ggcgattcaa ggcaagtaaa accggaagca
      181 gatcaagggg ttgcagtagg ttctaaagca acggtaaaga acaaagctaa acaaagggtt
      241 gtggtaggtt ctgaggcaaa ggtaaatgca gaacgtggta ttgcgattgg taaagaagcc
      301 aaagcagggg gcaaaacaac gaacacactg ttagatggac cggcatatta tgcggacgct
      361 attgccgtcg ggtatcaagc agaggctggc aaaggtggcg ccatcgcttt gggaaaacaa
      421 gcaaaagcaa cgaagcaaaa tggaatggca cttggcgtag aatcagaagc tgccggtgat
      481 tttagtaccg ccgtgggtaa tgaaagcaag gcaaaggggc aaggcggcgt aggtctcggt
      541 aaccaatcta aggcagaggc agattttgcg gtggctgtgg ggaataaggc tgaagcgact
      601 aaggaaaact cacttgtcat tggccgatat gcaagggcaa acggaaatca ttctgtgagt
      661 ttaggatctc gtagtgaaat aaaagatggt gtgagcaatt ctgtggcacc gggttatggt
      721 tcagtagcta gtgagaataa tgtcgtctct gttgcctata aagaaacacc acaatctaca
      781 gaactttcct atagaaaaat tgtaggtgtt gatgatggtg tcaatgattt tgatgccgtc
      841 aatgtacgcc agttgaaagc gatgcaagga caaaatatgg ctgagctatt ttcagtacgc
      901 agcgaagtgc gtggtgtggc agcgagtagt gcagccctat cggctctgac tccattatcc
      961 tatgatgcaa acaatcctac gcagtttatg gtcggtttcg gcacatacaa aggacgtcaa
     1021 gctatggcat taggattgtc ccatttcgtc aatgaccgct ttatggtaaa agtaggcgga
     1081 accttaggcg gcaataagac gggacatatg atgaatgtag gcttgacttg gaaatttgga
     1141 acgagccaag gtgttccggc tacaagtgat aaggtgctta tggaacagtt gatgcaagat
     1201 aatcgtcaat taaaggcacg attggagaaa ttagaagcac gtctgaatgc tatgtagcct
     1261 tgtggcgata acggtttatt gagtatatcg ccaatgataa agtgcataat tttcaaaccg
     1321 ttgataatat acaataaatt gtaaccaata cctcaaacgg gtttgaaccc gaatatgcct
     1381 gacaggaact ttctgtcagg cattgttgtt gtgatgttct gcaatcattt tttggtacac
     1441 ggccggcggc acatatcttt tgacggtttc catccagccc tcgggcccga ccagtccttt
     1501 gatcatagtg gacgacactt cggcgatttc gcgcggcggc atgaggaata cggtagatat
     1561 ttcgggtgcg aggtcgctgt tgatatggcg catggagcgt tcgtattcat aatcggaggt
     1621 ggaacggatg ccgcgcacga tgaatcccgc atctacctca cgggcgtaat gcaccaaaaa
     1681 ccggttttga aacacttcaa ttctgacgtt tgggaagttg tcggtaatgg cgcacaacat
     1741 atcctgcctt tcagcggcgg tataggtgtt gcgtttgtcg ggattgatgc cgatggcgac
     1801 gatgagttcg tcaaacatag attgcgcctg ccgtatcatc cacagatgcc ccaatgtggg
     1861 cggatcgaaa ctgccggcat aaacggcgcg gcgcggtgta ttcggtaaca tttgattcct
     1921 cccggcttca tagccggctg tgtttttatg tgtttgcatc cgtattgtat gcccaaaaca
     1981 aaatgccgtc tgaaaataag tgctttcaga cggcatggtt tgcatggtgt ttcgcctaca
     2041 tcagtcgtcg ccgtttaagg acagcaggat gttgagcagg ctgctgaaga tgttgtaaag
     2101 cgagataaac agtgtcagtg ccgcgctgat gtaactgtct tcgccgccgt cgatgacggt
     2161 gcgtacctgc cacataatca ttaaggaact gaacaagaca aaaccggcgg aaatggtcag
     2221 ggcgagtgcg ggaataccca aaaacagatt ggcaaccacg gcgaccatca gaatgaccgc
     2281 acctacggtc aggaagcgtc cgagcgcgtt catatcgagc cgggttcggc gtgccaatgc
     2341 ggacatcgtt aaaaagacgg cggcggtcat cgcggcggca atgccgacga ttttcgcacc
     2401 gtcggcaata tggagtgcgt attgcagcac ggggccgatc aatacgccca taccgaatgt
     2461 gaataccatc agcagggtaa cgccggtatt gctgtaacgg tttttttcga tgaagtggat
     2521 cataccgtag aaaaacgcca atacgacggc aaatcctatc cagcgcgaac cgaaggcggc
     2581 gtaaaaattg aaaccggcat tggcggcaag tgccgcgcct gcggctgccg gaatgaatga
     2641 aaatccgagc aggcggtagg ttttctgcag gacggtgttt ttagaaaccg tatgcgcggt
     2701 gtagtcgtaa acgtcgtgtt gcatatcatc tgctcctgaa agcgcggttg gaaataacgg
     2761 gggattttaa cattgcccaa tgtcaaaatt tgtccggttg cgtgaagata aagttgtccg
     2821 gcgtatttta aaggccgtct gaagcagttt cggacagcct gtgttcaaaa cggaaaaccg
     2881 ttattgcgga acgtatccct gcacggcatc cgcgccgtcg ccgaagaaat actgctccat
     2941 ctgctgagcc aggtattcgc gcgcgcgcgg atcggcaagg cttaagcggt tttcgttaat
     3001 cagcatggtt tggtggcgcg tccacgccgc ccatgcttct tgcgatacgt tttcaaaaat
     3061 gcgtttgccc aattcgttgg gcagcggcgg aaatttcatg ccttcggctt ctttgttgag
     3121 cttgacgcag aataccattc gtgccatagc ggattccttt gctgtgttca gaaataacgg
     3181 ggcgatttta accgattagg gggtacagac aaaagccttt ttattcccga tgataaggat
     3241 ggttgtgcag gatggaaacg gcccggtaga gctgctcggt cagaaggacg cgcaccatgc
     3301 cgtgcggcag ggtgaggctg gacaggcgca tcatcatgcg ggcttgttgt ttgaggcggt
     3361 cggtcatgcc gtccgcgccg ccgatgacga agcagacgtg ttcgccgttt tgccgccagc
     3421 ttttgaggtg ttccgccagc tcgacggagg tcggtgcttt gccgcgttcg tcaagaacga
     3481 cgaggaacgc gccttgcgga atggcttcaa ggatgcgttt ttcttccgcc gccatacctt
     3541 ggacggcatt cacgcccgcg ccgcgttttt cgggtttgat ttctttgagt gcgtaggcgg
     3601 cgtcgcgtcc gaagcgtttg gcgtattcgg cgacggcctc atcaacccaa cgcggcattt
     3661 tggtgccgac tgccaagacg gtaatgttca atgctttccc ccttacagga aatgccgtat
     3721 gaatattcag acggcatcgg gaatcagtct gcagcgtgcc acggcttctg cattccggcg
     3781 tggaaactcg gtttctcgcc gccccagatg gtgtcaatgt cgtagaagtc gcgcacggca
     3841 gggagcatga cgtggacgac gaggtctcct gcatcaacca acgtccattc gccgctgtct
     3901 ccttcggtac tgaggatttc aaaaccggct tctttcaaat cgacggcaac gttgttggcc
     3961 agtgctttga cttggcgcgt actgtcgccg ctggcgataa tcattctggc aaacagcgaa
     4021 gttttgtctt gcgtttcgag aacggaaatg tctttggctt tgatgtcttc gagggcattg
     4081 acggcgaccc cgaccatttt ttgcaggtct tgcagttctt gttcgttcat tgttttccta
     4141 acgggatgtt ttcagacggc attatagcct ctccttactg atttgacttt atttttcata
     4201 caaaccgtgt tttcggatat aacgggcggc ggcaggcggg atgccgtctg aaacaccggc
     4261 ggcactcagg ttgcggcgga tttccgttga cgacacatta tgcatcgggg ctgacaagat
     4321 gcggacgctg ccgtcctgaa gggcattgcc cagccacgcg tgcagttcgc gcggggtttg
     4381 gtgcaggctg tcgccctgcc gcatggcgac ggcgatattg gtttcgcgca cgagcaactg
     4441 ccattttttc caagtatgca gcttcagcag gctgtcgctg cccatcagcc accaaagttg
     4501 cgcggacggg aactgccggc ggaagatttg gacggtatca aaagtatagg ttgcactttc
     4561 tcggacgatg tcgcagtcgc tgacggcaaa acgcgcgtct tctgccgtcg ccagttcgac
     4621 catggcaagg cggtcggcgg cggaagcgga ggcggcgtct ttgtgatacg ggccgcctgc
     4681 cggcaggaaa acaaccgcgt ccaacccgat ttcgtcggca aaggcacggg cgatatgaaa
     4741 atgcccgttg tgtatcgggt cgaaagtgcc gccgaacagt ccgattttct tcatgatgtt
     4801 tccattcctt ccgataagtc catgccgtct gaaacactgc cgtccacaac cagcgtcagt
     4861 ttgccggtaa cgggatggat gaccagtccg tgaacggcga tatggcgcgg catcagcgga
     4921 tggttgcgga taaggtccac cgtgtggcgc acgctgtctt cgacgttgtc gaaaccggtc
     4981 agccagccgt cgaggtcgac acctgcataa cgcagggttt cgatacggtc ttcgggaatc
     5041 cggctttccc ggacgcgccc gaggaattct tcggcattca gcccctgcat accgcaatcg
     5101 tgatgggcga tgaccataat ctctctgacc ttcaattcaa acacggcaac caaaaggctc
     5161 cgcatcaccg aaccccacgg gtgcgtaacc agcgcgccgg catttttaat cagcttggca
     5221 tcgccgtttt tcaaacccaa cgcgtcgggc agcagcccga taatccgcgc atccatacag
     5281 gacaaaaccg ccagcccgcg ttcggggtat ttgtcggtaa agtatttttc atatcctccc
     5341 gactcgacaa acttctgatt ataggaaagg atttcgctca actcgctcat tttgccgtcc
     5401 tctgaaaaag ggttcgcatt ataacgtttc cgtccgtttt ccgccttcgc cgttgtccaa
     5461 cagcaggaaa atgcacaccg caaatgccga caacagcaat gtcaacacca ttgcccgcgc
     5521 ataattgtcc tcacccgcac gccccaaata ggcataaatc aaagtcgtca acgtctgcca
     5581 ttccggacgc gacaggaaca aggttgccgc aaattcgccc acacacgtcg ccgccgccaa
     5641 agtcagaccg cgccgcaacg ccggtttcaa gagggggaac gtgatacggc atgccgtctg
     5701 aaagccgttt gcgcccaaac ctgccgccgc cctgccgtaa tccggcggca gtgcatccca
     5761 ggccgataaa acatcttttg ccacaaacgg atacgccagc agcgcataca tcgccagcag
     5821 cagcggtaac gaagccgtcc accccggata aagcagcagc acgcccgccg aaacacaaac
     5881 cggcgacacc ataaacggta aaaacaccag tccgcgcatc cacgccagcc gccgcgccgc
     5941 cgccgcatac accacaccca aaaccgccgc cgcaaacacc gccgccgccg aaaagcgcaa
     6001 agtattccac actgcctgcc acgtttcact ttccattaac acacgccgcg attcgccggc
     6061 cgaccacgct ttcacaacaa ttgccgacaa aggaaacagg cagcacacgg acaacaccgc
     6121 caccgaaaat gccagcaata catattcccc caccgattgc ggcggcgacg gcatcacggg
     6181 ggaaaccgcc ttatccgaaa ccgcgcgcct gccgaaccac gcatacagca accctgccgc
     6241 cgccgttacc cccaacacca gccacaccag cgccgaagcc cccgccatat cgagttcgaa
     6301 cataaccaac tggtaaattt ccacttcgac cgtggcataa cggctgccgc ccaacagcaa
     6361 tgccagcccg aaccccgaaa aacaatacag gaagacaagg cacacgccgc cggcaagcca
     6421 cgggcgcaaa acgggcattt caatgtccca aaaccgccgc cacgcccccg cgcccaacgt
     6481 ccgtgccgtc tgaagccgtg ccgcaggcac ttgagcaaac ccctgatacg ccgccctgac
     6541 caacacgggc aggttgaaaa acacattgcc gtacaacaac agatacggcg tatcctgccg
     6601 gccgcgccac aacagcccgt ccgccccgaa cagagccagc acgcccacgc ccgccaccag
     6661 cgtcggcatc acaaacggca gcatcagcag gcgcagcacc aaagcccgcc ccgggaacgc
     6721 cagccgcgcc agcacccacg cgacaggcac gcccaaaggc agcaccagca cacaggttgc
     6781 cgccgcctga aacaccgtcc acgccaaacg tttgagcata taggcatccg acagcaccgc
     6841 gcgccacgcc aaaccgtcat acgccgccac cgcccacaaa ggcgcaacga ccattaccgc
     6901 caaaaaagcc gaaggcagca gggaaaaagc accccgtacc gcccaacgcc gtccatccat
     6961 cgccttcccc acttgaaaca ctgatgttgc gattgtaccc aaaagcccca cataccgtat
     7021 atttcaatcc gactacgtac cgtatccgcc ttcctcccgc cgtctgaaat atagcggatt
     7081 aacaaaaatc aggacaaggc ggcggaccgc aggcagtaca aatagtacgg aaccggttcg
     7141 cccggtgctt cagcacctta gggaaccgtt ccctttgagc taaggcgggg caacgccgta
     7201 ccggtttttg ttaatccact ataaatcgtt caaataaaca ggaatataac ttcagacaaa
     7261 caacttaccg cccgatttgt gctatcgttt tcgcacaact taaaaaaacc tgacaatttt
     7321 gtacttttat tacagagaaa ggctttacaa atggacggcc ggacacagac gctgtccgcg
     7381 caaaccctgt tgggcatttc ggcggcggca atcatcctca ttctgatttt aatcgtcaaa
     7441 ttccgcatcc gcgcgctgct gacactggtc atcgccagcc tgctgacggc tttggcaacc
     7501 ggtttgccca caggcagcat cgtcaacgac gtactggtca aaaacttcgg cggcacgctc
     7561 ggcggcgtgg cgcttctggt cggtctgggc gcaatgctcg gacgtttggt agaaacatcc
     7621 ggcggcgcac agtcgctggc ggacgcgctg atccggatgt tcggcgaaaa acgcgcaccg
     7681 ttcgcgccgg gcgttgcctc gctgattttc ggcttcccga ttttcttcga tgccggacta
     7741 atcgtcatgc tgcccatcgt attcgccacc gcacggcgca tgaaacagga cgtactgccc
     7801 ttcgcgcttg cctccgtcgg cgcatttatc cgtcatgcac gtcttcctgc cgccccatcc
     7861 gggcccgatt gccgcttccg aattttacgg cgcgaacatc ggccaggttt tgattttggg
     7921 tctgccgacc gccttcatca catggtattt cagcggctat atgctcggca aagtgttggg
     7981 gcgcgccatc catgttcccg ttcccgaact gctcagcggc ggcacgcgag acagcgaccc
     8041 gccgaaagaa cctgccaaag caggaacggt cgtcgccgtc atgctgattc ccatgctgct
     8101 gattttcctg aataccggcg tatcggccct catcagcgaa aaactcgtga gcgcggacga
     8161 aacctgggtt cagacggcaa aaatgatcgg ttcgacacct gtcgcccttc tgatttccgt
     8221 attggccgca ctgttggtct tgggacgcaa acgcggcgaa agcggcagca cgttggaaaa
     8281 aaccgtggac ggcgcactcg cccccgtctg ttccgtgatt ctgattaccg gcgcgggcgg
     8341 tatgttcggc ggcgttttgc gcgcttccgg catcggcaag gcactcgccg acagcatggc
     8401 ggatttgggc attcccgtcc ttttgggctg cttccttgcc gccttggcac tgcgtatcgc
     8461 gcaaggttcg gcaaccgtcg ccctgaccac cgccgccgcg ctgatggctc ctgccgttgc
     8521 cgccgccggc tttaccgact ggcagctcgc ctgtatcgta ttggcaacgg cggcaggttc
     8581 ggtcggttgc agccacttca acgactccgg cttctggctg gtcggccgcc tcttggatat
     8641 ggacgtaccg accacgctga aaacctggac ggccaaccaa accctcatcg cactcatcgg
     8701 ctttgccttg tccgcactgc tgtttgccat cgtctgacag acggaaagga tagtaaatga
     8761 ctacgcattt tgtcgttacg ggcgtatgcg gctgcggcaa gaccaccgcc gcgccgtccc
     8821 tgcagaaaca cctcggtcaa tgtccctatg ccgaaggcga cgagttccac acccaagcca
     8881 accgcgacaa gatgggcgcg ggtattccgc tgaccgatga agaccgctat ccgtggttgg
     8941 gcaatctgcg cgactggatg acgcaacagg cgcaaaacgg cgcggaccac accatcgtaa
     9001 cctgttccgc cctcaaacgc ggctaccgcg acattctgcg cggagccgaa ggcaaagccg
     9061 ccttcatcca cctcagtccg ccgcaagaca tcaacctcga acgcatgatg tcgcgcaaag
     9121 ggcactacat gaaagcaggg atgctcgatt cgcaactgga aatcctcgag gaactgggcg
     9181 aaggcgaata cggggtcaaa atcgccaacc ccggcacgcc cgaagcggtc gaagccgata
     9241 ttctgaactg ggttgcctcg gaaaacctgc tttgaagcaa tatgccgtct gaagcccggc
     9301 acaggatggg tttcagacgg cataaacatc gggaacagaa tgaattacat tgatttaaaa
     9361 tggaaaatac ccggctatcg tctcattttc gttttaatca gccataaaaa tgccgtccga
     9421 aaccctttca gacggcattt ctgtcaaacg ccggacgcac tcaatccaaa ctcaacagca
     9481 ggttgcggaa cgcgttcggg tctttgataa acgtcatctc gcccgcctgc ggaaaatgaa
     9541 aatccaacag gcgcgacacc caaaaacgga tgcagccggc gcgttgggcg gtcgggaaat
     9601 acgccttttc cccggcactc aaggggcgca cgccctcata accgccgata aacgcctttt
     9661 tcaacgcctc atccaactta ttgtccgccg tccttgccca atcgttgacc gcaatcgcca
     9721 agtcatacat aaaattgccc cggcaggcat aatagaaatc gatgaagccc gatacctgac
     9781 cgccgtcaag caacacattg tctttaaaca ggtcggcgtg gatgatgccc gaaggcagat
     9841 ggttgccgag attgtccttc agcgcatcga tttcggaaca cagcagcgcg gcatcgtctt
     9901 gcgacaggac gggcagcagc cgggcgcacg cttccgtcca ccacgcatcg taacgcgggt
     9961 tttccatttc caaagggaaa tcggcggcgg caaggtgcat tttcgccaac atcgcaccgg
    10021 tatggaaaca ctgctcagcc gtcggcagcg cggtatccga acctttcagg caggcaacca
    10081 ggcaggccgg cttgcccgcc aaaacggaat cgagccggcc gtctttgcgc gcaaccggcg
    10141 cggcaaccgc cacgcccttc atactcaaat gccggttaag ctccagaaaa aacgacagct
    10201 cttcctgttt caacacttca aacacggtca gcacataacg tcccgaagtc gtcgtcagaa
    10261 aataattgct gttggtaatc ccctgcgcaa tgccctgcag ggaaacaaat tcccccaaat
    10321 cgtaaccgct caggaagccg cgcatttcat catcggaaac actggtatag acagacatat
    10381 caacttactt gctcaaaaac gggcaaaccc gccgtcagaa cgccggacgg caggcaaaca
    10441 tataattcac atcggtcgaa tcgcacaggg cataagtttg cgacaacaca tggtaagtca
    10501 tacccttcgt atccgccaca tccaagcccg cctgacggca catccgcgcc agctcggcag
    10561 gcgagatgaa ttttttccag tcgtgcgtac ctttggggac aaacttcaac agatattccg
    10621 ccgccacaat cagatgcagg tacgatttcg ggtttttatt gatggtggaa aaaaacacca
    10681 tgccgtccgg cctgaccagc ttggcacagg cacgcacgat ggcggcggga tcggggacgt
    10741 gttccatcat ttccatgcac gttaccgcat cgaacgagtg cggttccgcc ccggcaaggt
    10801 cttccacgcg gacacattcg tattcgatat cggcgacatt gttcaaagcc gcgtgcaggc
    10861 gggcggtttc caacgactgc tccgccatgt cgataccttt gacaaacgcc gcgccgcgcc
    10921 gcgccatgct ttccgcgagg atgccgccgc cgcagcctac atctaaaacc cgtttgccgc
    10981 gcaaatccgc gtgtccgtcg atataatcca gccgcagcgg attgatgtcg tgcaaggttt
    11041 tgaactcgcc cgacttgtcc caccatttgt cggcaatccg gctgaatttg gcgatttccc
    11101 cctcatcgac attatatttt ttgtcggaca ttttccctcc catctgacga accgcccact
    11161 ccaaaaccca agattatact atggaacaca atgccgtctg aaagcctttt cgggcggcgc
    11221 ggcgcaaatg ccttacaaat ccttacactt tacggcataa tggcggctcg ctttttctgg
    11281 cagaaagaca aaatatgccc accaaaaccc cttcactgtt cggcggcgcg atgattatcg
    11341 ccggtacggt catcggcgca ggtatgctcg ccaaccccac cgccacctcc ggcgtatggt
    11401 ttaccggctc gctggccgtg ttgctgtaca cctggttttc tatgctttcc agcggcctga
    11461 tgattttgga agtcaacacc cattatccgc acggcgcaag tttcgacacg atggtcaaag
    11521 acctgctcgg acgcggctgg aacatcatca acggcatcgc cgtcgccttc gttttatacc
    11581 tgctcaccta cgcctacatc ttcgtcggcg gcgacctgac cgccaaaggc atcggcagcg
    11641 cagtaggcgg caaaatttcg ctcaccgtcg gacaactcgt cttcttcggc atcctcgcct
    11701 tttgcgtatg ggcatccgca cgcttggtcg accgctttac cggcgtcctc atcggcggca
    11761 tggtattaac ctttatttgg gcaaccggcg gcctggttgc cgatgccaaa ccgtccgtcc
    11821 tcttcgacac ccaagccccc gccggcaccg gctactggat ttacgccgcc accgccctgc
    11881 ccgtctgcct cgcttccttc ggcttccacg gcaacgtttc cagcctgctc aaatacttta
    11941 aaggcgacgc gcccaaagtg gcgaaatcca tctgggcagg tacgttggtt gccttggtaa
    12001 tttacgtcct ctggcaaacc gccatccaag gcaacctgcc gcgcaacgag ttcgcccccg
    12061 tgattgccgc cgaagggcaa gtctccgtcc tgattgaaac cctgtccaaa ttcgcccaaa
    12121 ccggtaatat ggataaaata ttgtccctat tttcctacat ggcaatcgcc acctcgtttt
    12181 taggcgtaac cttaggcctg ttcgactaca tcgccgacat cttcaaatgg aacgacagca
    12241 tgtccggccg caccaaaacc gccgcgctga ccttcctgcc gcccctgatt tcctgcctgc
    12301 tcttccccac cggcttcgtt accgccatcg gctgcgtcgg cctggcggca accgtctgga
    12361 caggcatcat ccccgccatg ctgctctacc gttcgcgcaa aaaattcggc gcaggcaaaa
    12421 cctataaagt ttacggcggc ttgtggctga tggtttgggt cttccttttc ggcatcgcca
    12481 acatcgccgc acaggtattg agccaaatgg aactcgtccc cgtatttaaa ggataaaggc
    12541 aaaatgccgt ttgaaacccg ccggcggctt caggcggcat tgccgcaaca aacggcaacc
    12601 gtattccggc acacagcgca ttaccctacc ccctcacgca caaatcccgc cccgacaggc
    12661 gcgggacgca accataaagg aacaatgatg aagctcaaaa tagacattgc aaccaacaac
    12721 ttcaaacacg gcggcggcac ggaacgctac acattggatt tggtaaaggg tctgaacaga
    12781 caaaacatca caccggccgt ttatgcgacg aaatttgatc acggcattcc cgaatacgcc
    12841 atgatcgaac cccatcttgt cgatcaacgc cggacgctga aaaaactacg ctcgttcctc
    12901 ttttcaagcc ggctcgctca aaccagaaaa aacagtgccg ccaaactgat tgcctgccac
    12961 cacgccgatt acgccgacct cctcatctgc ggcggcacgc acttgggcta cctgcaccat
    13021 atggcgcaaa aaccgaacct gctcgaccgc ctcgccatac gccgcaaccg cagcaactac
    13081 gccaccgcca aactgattgt ggcgcattcc catatgatgc ggcgcgaact ggtcggacta
    13141 tacggcgttc cccccgaaag aatccaagtc gccccccccc cgcagatacg gaacgcttct
    13201 ttccacaacc tagagaaact gccgccctgc gcgccaaata cggttttgcc gaccatgaaa
    13261 ccgttttcct attcccatcg accggccaca cgcgcaaagg cctggaactg ctcgccgact
    13321 ttttcgaaca aaccgaactg cccgtcaagc tcgccgttgc cggttcaccg cttccccgcc
    13381 cgatgaaaaa cgtcgtcgga ctgggcttct gcaccgatat gcccgaactc taccgcgccg
    13441 ccgactttac cattatggca tccctgtacg aacccttcgg gctggtcggt gtcgaatccg
    13501 ccctatgcgg cacacgcgtc gtcctctccg aaaacatggc atgtacagag gtcatgaacg
    13561 aagaagccgg attctttttc tcacgacaaa acccggaaac cctcgcgcaa gccgttgccc
    13621 gggccgtcag ccttaaaaaa cagggcggac accgcctgtc cgacccgatg cgggcgctga
    13681 actacaaccc gtccctgtcg caccacatcg accgactgac cgacatgctg gcatccgtat
    13741 aacaaaatgc cgtctgaacg ggttttcaga cggcattttc gcaaccgttg cagccgtgcg
    13801 gcaaaaagct aacgggcaat tttcaaaacg tcgtacagat agttgcggta atttccgacc
    13861 aactcccggt tcgggcagtc ttccaaccct ccgccgcaca aaccgcgcat cgccatattc
    13921 gcacgcatga agccgtcttt ggcttccaaa ccagccgcct ccatcagtac gccgcctgca
    13981 aaggcaaggt cgaggaaatc ctgccgctgt acgaatccgc cggcaatatt gctcctgacg
    14041 gcaaactgcg ttacataatc cggctgagcg taatcccatt tcttcctgac ggacacgccc
    14101 tcaaacggta cctgatgatc gccgaaataa ccgaaaacaa agggtttgcc gcgttcgtgc
    14161 agataacggt cgaaactttc caccgctttg tccaaatcgg caatgcgccc gatgtagtcg
    14221 ttaagcgcgg atacggtttt cgcattcaaa tcgggcgcat ccaaatcaaa cacattgtcg
    14281 gtatccgtcc gatacggccc gtgctccttc atggtcagca catatacgaa catcggctgc
    14341 cgcacgtttt ccaaatcggg atggcgtttt tcgagaatca tccgcgcata ctgcatcatt
    14401 tcctcactgg aaatgtgcca caggtttttg cccatcggcg cgggatagcc caaatcttgc
    14461 ggctggaaca tcagattaaa gccgaaatgg tcatatgctg ccttggcatt gtagttgccc
    14521 ttggtaaacg gcgagagcgc cacgcaaaaa taaccgtgtt cgcgcaggtt gcggacaaaa
    14581 ccggtctgca aatgcggtac gaccgaataa aacacgccgc ttgccaacgc gccgaaatct
    14641 gtggacggaa cacccgccaa aaacgcaaat tcggacttcc acgttgcgcc gccgaaagta
    14701 tgcacgcgca aaggtgagga aaatacagta tcttcctgcc gtccgaacat tttcaaatcg
    14761 ggaattttgg cggcggcaaa atcgaaacag tgcggatcga gcgtcgattc catcagggta
    14821 acgacaatat cgggcttttc atccgacatg ccgtacggcc gggtttcaga cggcatctgc
    14881 ctggcaaacg cttccccgtc gccctcgaac acgggtactt ggaaaaacac cgcccgacag
    14941 gacatcggca ggttcaaaaa cacgtcgcgc ccgtcgtccg gcagcgagtc cagccacgtc
    15001 tttaccgcgc cggggtgctt ggaaaaatgc cgcaccgaca cgaacgccgc cgcaaacaga
    15061 accgcgcccg cccaacgcca cggcataccc aaagaatccg caccgctcca gccgaaaacc
    15121 gcatatgccg ccaaagccag cagccccgcc ataccgataa ccgcttcctt ataatgaaac
    15181 agcgtttccc aattccgcca gtcggcaatc agcaaaaagt cggaaatcaa cagcggctgt
    15241 ttgtaataat ggattttcag cctgtggaac agtatcagca ccacaaacca gaccgaagcg
    15301 aaattcaagg cgcgctgcca ctgcgccgtc aacatcagca taccgcccgc caaaaagaca
    15361 aacagcgccg acgcgaaaaa atacgtccag cggtagtgcg acctgacaat cagcagcacc
    15421 gccgccgtta caaacaaaaa taggaaagca taggcaacca tcccgcccca ctcctcaaaa
    15481 cggcatacct gtgccgttgc gaattattat cgaaagcatg cattataatg cagcacaggc
    15541 gcatttccac cgatatttca gtataatgcc atccccgaac ccgcccaacc caaaggaacc
    15601 gtgatgaaac tcatcattct cgaccgcgac ggcgtcatca accgggaccg cgacgacttc
    15661 gtcaaatccg ttgacgagtg gatacctgtc gaaggcagca tggatgcggt ggcattcctg
    15721 acgcaggcag gctgcaccgt tgccgtcgcc accaaccaat ccggcatcgg ccgcaaatat
    15781 tttaccgttc aagacctcac cgaaatgcat gccaaaatgc accgcctcat ccgtcaggcg
    15841 ggcggggaaa tcgacggcat ctggttctgc ccgcataccg gcgccgacgg ctgcaactgc
    15901 cgcaagccca aaccgggcat gattgaagac atcctcgaac gcttcaacgc ccaagcttcg
    15961 gaaacttggc tggtcggcga cagcctgcgc gatttgcagg caatcgatgc cgtcggcgga
    16021 aaacccgcgc tggttctgac cggaaaaggc aaaaaaacgc tctcccaaca cggacacgaa
    16081 ttgcccggac acacacaggt tttcgatacc ctgctcgatt tctcacaata catcatgcag
    16141 gaaaacgccg caccgcaagc cgactgaaca taccgcattt ccgacaaggc aaaaccatgc
    16201 tcatcatccg caacctgatt tactggctga tactctgttc cagcctgatt ttcctctttc
    16261 cctttatgct gctcgcctcg cctttccggg acggggcgca caagatggcg cgggtctggg
    16321 tcggcatcct caactggtcg ctcaaacaca tcgtcgggct caaataccgc atcatcggcg
    16381 cggaacacat tccggaccgc ccctccgtca tctgcgccaa acaccaaagc ggctgggaaa
    16441 cgctcgcgct ccaagagatt tttccgccgc aggtttacgt tgccaagcgc gagttgttca
    16501 aaatcccctt tttcggctgg ggcttgaaac tggtcaaaac cataggcata gaccgcaaca
    16561 accgccgcga agccaacgaa cagctcataa aacagggttt ggcgcgcaaa aacgaaggtt
    16621 attggattac cattttcccc gaaggcacgc gccttgcgcc cggaaaacgc ggcaaataca
    16681 aactcggcgg cgcgcgcatg gcgaaaatgt ttgagatgga catcgtcccc gtcgccctca
    16741 acagcggcga attttggccg aaaaattcct ttctgaaata tccgggggaa atcaccgtca
    16801 tcatctgtcc gaccatcccg cacgcaagcg gcagcgaagc cgaattgatg gaaaaatgcg
    16861 aacacctcat cgaaacgcag cagccgctca tttccggcgc aggcccgttt gccgccgaaa
    16921 tgccgtctga aaccgcatga cctccctgat tcacaccctt tcagacggca tagaactgac
    16981 cgtcgaaatc aaccgccgtg ccaagaaaaa cctgattatc cgccctatcg gcacacatac
    17041 cgtccgcatc agcgtcccac cctgcttctc cgtctccgcc ctcaaccgct ggctgtatga
    17101 aaacgaagcc gtcctgcggc ggacactggc gaaaacaccg ccgcacaata cggcaaaccg
    17161 actgcccgaa catatctggt tccacggcag gcagcttgcc cttaccgccc atcaagacac
    17221 gcaaatcctg ctgatgccgt ctgaaatcct cgtccccgaa ggcgcgcccg aaaaacagct
    17281 tgcgctgctg cgggactttt tggaacggca ggcgcacagt tacctgattc cccgtctcga
    17341 acgccacgcc cgcaccacgc aactcttccc tgccgccgcc tcgctgacct ccgccaaaac
    17401 cttctggggc gtgtgccgca aaaccacagg catacgcctc aaccggcggc tggtcggcgc
    17461 acccgaatac gttgccgact atgtctgcat acacgaactc tgccacctcg cccatcccga
    17521 ccacagcccc gccttttggg aactgacccg ccgcttcgcc ccctacacgc ccgaagcaaa
    17581 acagtggatc aaaatccacg gcagggagct tttcgccttg ggctgacaca gccccgggcc
    17641 gacgctttca gacggcatcc gtgccggaac aggcacgcgc ccgcccgatt caaaccgcga
    17701 tgacgctttg ccgccggttc ggggcaggat ggcggcatcc gcgccgtccg caccgtttca
    17761 tttcacaccg cccttccgaa acccgcccga tccgacgtgc ggtatgaaac gcttgagccg
    17821 gcgcgaagtc ttctactgat ttgcccgcga aaataccgtc cgaaaggttt tcggacggca
    17881 tttttttgcg ttttcctgga tggcggcgga ttcgtaaaag gcgggcaggg tggattgcag
    17941 gatgggttga ggcctgccaa atccgccaac tctaccgaat acgccgccgt cattcccgcg
    18001 caggcgggaa tttggttttg tcggtgcgga aacttatcgg ataaaacggt ttcttagatt
    18061 ccacg
//
LOCUS       DAWTHD010000036        17958 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWTHD010000036 DAWTHD010000000
VERSION     DAWTHD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17958
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.036"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..1477)
                     /locus_tag="WM704_000941"
     CDS             complement(<1..1477)
                     /locus_tag="WM704_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340386.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGRS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAMDVE"
     gene            complement(1477..2232)
                     /locus_tag="WM704_000942"
     CDS             complement(1477..2232)
                     /locus_tag="WM704_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340387.1"
                     /translation="MSMYMDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVR
                     QFAQAVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGI
                     LLNRRTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAF
                     VSILYLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGL
                     IESCVDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            complement(2229..2834)
                     /locus_tag="WM704_000943"
     CDS             complement(2229..2834)
                     /locus_tag="WM704_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340388.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            complement(2824..4314)
                     /locus_tag="WM704_000944"
     CDS             complement(2824..4314)
                     /locus_tag="WM704_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFA9340389.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAAKQDVNLHQKDSDMEFVPAKVNGTKADAEDEPKEAEPITLEQ
                     TSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAGNAIYAIDVLH
                     EEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDKYDEYTNCGIG
                     NKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            complement(4311..5198)
                     /locus_tag="WM704_000945"
     CDS             complement(4311..5198)
                     /locus_tag="WM704_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFA9340390.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQKEVQS"
     gene            complement(5439..5621)
                     /locus_tag="WM704_000946"
     CDS             complement(5439..5621)
                     /locus_tag="WM704_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340391.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            5621..5956
                     /locus_tag="WM704_000947"
     CDS             5621..5956
                     /locus_tag="WM704_000947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340392.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(5993..6652)
                     /gene="ung"
                     /locus_tag="WM704_000948"
     CDS             complement(5993..6652)
                     /gene="ung"
                     /locus_tag="WM704_000948"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFA9340393.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            6733..7140
                     /locus_tag="WM704_000949"
     CDS             6733..7140
                     /locus_tag="WM704_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFA9340394.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            7155..8102
                     /locus_tag="WM704_000950"
     CDS             7155..8102
                     /locus_tag="WM704_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HFA9340395.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(8209..9279)
                     /locus_tag="WM704_000951"
     CDS             complement(8209..9279)
                     /locus_tag="WM704_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFA9340396.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            9464..9739
                     /locus_tag="WM704_000952"
     CDS             9464..9739
                     /locus_tag="WM704_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFA9340397.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            9741..10364
                     /gene="lipB"
                     /locus_tag="WM704_000953"
     CDS             9741..10364
                     /gene="lipB"
                     /locus_tag="WM704_000953"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFA9340398.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            10357..11340
                     /gene="lipA"
                     /locus_tag="WM704_000954"
     CDS             10357..11340
                     /gene="lipA"
                     /locus_tag="WM704_000954"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFA9340399.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            11815..12279
                     /gene="bfr"
                     /locus_tag="WM704_000955"
     CDS             11815..12279
                     /gene="bfr"
                     /locus_tag="WM704_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFA9340400.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARSELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            12307..12780
                     /gene="bfr"
                     /locus_tag="WM704_000956"
     CDS             12307..12780
                     /gene="bfr"
                     /locus_tag="WM704_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFA9340401.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            13108..13428
                     /locus_tag="WM704_000957"
     CDS             13108..13428
                     /locus_tag="WM704_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9340402.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            13477..16035
                     /gene="glnD"
                     /locus_tag="WM704_000958"
     CDS             13477..16035
                     /gene="glnD"
                     /locus_tag="WM704_000958"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFA9340403.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPVPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPVRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            16182..17645
                     /gene="guaB"
                     /locus_tag="WM704_000959"
     CDS             16182..17645
                     /gene="guaB"
                     /locus_tag="WM704_000959"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFA9340404.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTYKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
BASE COUNT         4928 a         4805 c         4052 g         4173 t
ORIGIN      
        1 gctcgacatc catggcatct aatgagtcct gtttttggtt attctgcttt tcaagataag
       61 cctgtaattt ctgatcctgc tcacgctgga atctaacctg tggggcgaca agagcagtaa
      121 agttaccttg cgaagcgcag cggcataggt taatcaaaag accaaggggg ctagtaactt
      181 cttttttctg tccctggatc tcatccaaga tttcctgcct caaagcccag gaactttttg
      241 agagatgttt tgtaaaaatc aaatccgcct gttgacgcat ttgcacatta aaaagttttg
      301 gataaatcaa atcaacatca tcaaccacta ccacatgatc ttgttcggaa gaacgtggtt
      361 tgttgggttc aacagtcatg tagttgtagt tataaacccc tttgtaattt atataataaa
      421 taatctgccg attcggcaat tctgaacttc cccaatcgac acttatgaat ttcccactac
      481 gacctttctt aaaattccaa ttcgacacat ccgaatattc cctttcgaca cttccggatg
      541 tcatacttgg gaataggggg gcatcaccct cgaaaccgct ctgaaaccct ttatccaatc
      601 catttggccc tttccgccaa tcacatttgg ccctttccgc caaactgaga atttcgcgtt
      661 ttttggagtt atatggacta aagccgtcgt cctgtttgcc agctagaagt tttttgatgg
      721 atataacaat atcgtgacca tttaccaggg taaaaatacg gcggctggcc aagaaatttc
      781 tctcataaat gagatttaaa ttcttcagcc tattgcgagc tgtcaattga gatttataag
      841 agagagaggt tctttcctcc caatcagcag cagtcaaagt agcaaaggca tgacgttgta
      901 aatgtggatt ttgagaaacc aaaccaaccg catcatttaa cacacacgac aataatacag
      961 ccgagttgat acagcctgta atagaaacca ggtctcggtg ataagcaatc cttttagaaa
     1021 agtgttttct aaaatacagg gaacttgtgt tctgtaaatc agccagcgta agtgcaagtt
     1081 gatcgtcagt accgctcgat ttagcgatca actcagctat ggcatccaag ttcactttga
     1141 ttgctaaagc agcccccaca cctttacggc atgtttgaat aaggtttgtc tcaagaagct
     1201 tgtttttaca agtgcgctgc tctctcactg tcaaaccagt ttcagcttcc atctgagcaa
     1261 tagacttgaa aatccaacca tcccgttcgg caacctcttt gcttactctt gtccaataga
     1321 ttaactgaga aagcattgca gcagtcttca gagagcctgt aatatcaatt aaacatcgat
     1381 agatggcaac ccttcgacca ccttgcaatg cttgccagca ttgtgtcagt tcagagttaa
     1441 atactttggg agggggtaat ttggtggcgg taaccatcac acacatccgt tcaaatattc
     1501 agaagtaaga tcagcaagca ttttcaagct gtatctacaa cgtcgcgctg ctggcatacc
     1561 atcaacacag ctctcaatca aaccaaaaga ggttaattcg gcaatagctc tttcctgttg
     1621 ccgtcttgac aacattgttg aataaaaaac gtgcttttca gctgcatgag tccaaggaga
     1681 ccagtcacca cgcttactca aatcttgata ttcagcttca ctttgaagaa acgacaaata
     1741 caaaatggat acaaaagcaa gacctctatc ggctaatgtc ttcaggtgaa gtttattaat
     1801 cgaaataggg ggcgaaacca tgtcaagtac atctgattta ccatgtttac ttaccaaatc
     1861 ctcaaaaaga gcatcattca atgtgaattg tgattcggtt ggcgctacgg ttctccgatt
     1921 aagcaaaata cctttctgca gaaggctatt gcgaatattc cgccactgct ttgcggtaat
     1981 acctgcttca ttaaacaaca gatcattatt caggcttacc cattcgccag gcagacattt
     2041 ctcgacaaac caactcaaaa aatatccctc tgaaacagaa ccgactgcct gagcaaactg
     2101 acgcacatat gtggccggtt cctcccacaa ggcaaccaag ctacggataa agttatcggt
     2161 tgacatgttc ttagatggta caaaaccagt ctcataatca taggaagtag agaaatccat
     2221 atacatactc atactccaaa gaactttaca tcgttaatta ttgaatgtaa ttgggcaagg
     2281 ctataatcgg ggaattcatc aatcaatagc tggtatctaa taaacaaatc tggctctttt
     2341 tccgacttac tccatgcctt caataccctg acgcgaacat caatagatgg catcacaatg
     2401 cgccctttaa cgacaggaat attcatttct atacgcaatt ttttaatttc cgccgtagaa
     2461 attaatgaac atagattcat aatcatcaga ttgctggcct tatgttctaa catccattta
     2521 agacgctgca tctgttccat aaacctttgt tcgtaatgtt ccatttctga aattttgctt
     2581 tttaaaacct tcagatcaat ggagtaggtc acaacatcag gacacatgga tgctaatttc
     2641 tcaatccgct tcgggttcat tcgtttgagc gtgttcaaaa catcctcatg ttcacgagca
     2701 accaaagcgt tataagaaga ttcacgcggc gtatttggtg cggatgccaa ccttgtaagt
     2761 gcaagattgg tataaaccgg atccgtatta aacagtctat aaatgggatt actaattaaa
     2821 ggcttactcc tcactcttag ctcccgtttt tctcaagttg ttgtactgtg tgctcagaga
     2881 taatatttct acgatattgg cgtgaaattc atggctggta ttagtttgcc aattcagtaa
     2941 agaggcaata atgttctctt tattaccaat tccgcagttc gtatattcgt cgtacttatc
     3001 ggggtcctcg tatgcaatac gaagcttaga atcactttta agtgtcagca ggggattgga
     3061 actatcacca acatcaaaca taaaggtgtc gattccacta agcttccata agaatgtgaa
     3121 aacatttccc gcttcttcat gcaaaacatc aatagcatag atggcatttc cggcacttga
     3181 tgctgaaata tgctcaaaag ccggatattc gacataccag ccgtatggaa agtccttatg
     3241 aatcttaaag caatcggaca gactcacttt ggctaacagt ttacgaacag cagaaaacag
     3301 ttttttgctg gtttgctcca aagtaatcgg ttcggcttct ttcggctcat cctctgcatc
     3361 agctttagta ccatttactt tggctggcac aaattccata tccgaatcct tttgatgcaa
     3421 attgacatct tgtttggcgg ctgtttcaat tttgtcttta tcagaagaat tttctgtttc
     3481 agaaaaggcg tcagagccat attcaaattt aaatctgtcg agcacgaact gataaagctc
     3541 atgcacgtct aactctgttg cagaatattg gttatgccac tgctccagta catccgacca
     3601 caaatattgg aaagaatcgt ccaactgaat tgatttagaa attgagaata gggtagagtg
     3661 aagtttttta atttcttcta cttttgcatg ggaaagtttg aatgcgtatt cttgcagctc
     3721 ttgtaaatta tcggaggcaa aaaagaataa tcctaaagtc ttatgagaaa ccgtaaggcc
     3781 tttatcagaa aacatcactt ccagttcgcg caaagaaagt ttccctgagt cagattcatc
     3841 ttggaaaata tcgcggattt cggcatattt acaggcctta tcccaaaaac acatttcagc
     3901 acggagttca ttttcaacaa gatgcgcaac ctgcaatgta gtttcgtcag agtaggggat
     3961 gtaaatacaa ggcatcatac ggaagcggtt atcaccagtt tcttccaaga gttcgcacaa
     4021 gattttgaga cgggtattac caccctgtgc cagtacatat ttactttcac caggtctctg
     4081 ggtaacgtgt acaggttgtt gaatacctga agaacggatg gactctttga tggccgcgta
     4141 gctggaaaca tcatgtaaac ggcgagggtt tttgtcaaag aaatcgattt cgctgattgg
     4201 caacgacatt aacatgcccc gagaaggagt atcctcttgg ccaaaactca tatttgtctg
     4261 tgcaggggta ccgatgtgca ggctcaaggt tgctttattt tggtccaaat tcatgattgc
     4321 acctcctttt gcagcaggct ggccatgtca ttgaaacagc ttccacgcag gttcttctgt
     4381 tcgataccag ggaaaatctc ataaacaata cggtgcatga tttcataagc aggcagcatc
     4441 tttccaagct gtttacgctc atgacaatga accgggactc ttaaagttgt agcttcctta
     4501 tatgctttgg cagcaggaac tgctacgctt aacaatttac gtctgtcatc tagagtattg
     4561 ctaaagaaat ctttgataga agatgcgatg aatttcgcat cattggtacg atcttgtgcg
     4621 tagatgatgg ctcgtaattg aggcatcggg atacgcattg cttcgccttt ttgcagacgc
     4681 tttagaagtt cttgcgtacc ggtaaggaac tcacgggcac tcaaaacttc gggcataatt
     4741 gggcaaagca ggcatgtagc agcaaatgct gcggtatcta agagaggacc aactgcaccc
     4801 tgagtatcga ttaaaaccac atcgtagtta ttctgtatgt attgggaatg cagtttgctt
     4861 ttgagcaaga aggcacggtc gggacgagag gttacttcag tctcgacaaa agcagaaatg
     4921 ttattggaaa taacaatgtc taaattcgga aaaacggtat tggaaattgt cgagcggatg
     4981 atttcttcgg tattttcacc gagaagtaat tcaacaatac catttggagc gcggtggctt
     5041 attggatagt attttgacaa tgatggctga acatcgcagt caatcattaa gacacgcagt
     5101 ccgatatcag cgagaacagc agagacatta gcaatgagtg ttgttttacc tacgccacct
     5161 tttgtagcgg ctgcagacaa tattacgggt gcggacataa tatttcctta tatttaattg
     5221 atttataaag agaaaaacat ccgcaatcga acaggtttaa aatatgaaat ttacatattt
     5281 attctagttc gcataatgta tattatgtta aatttataat ggattgaata gatattttta
     5341 caatctcatt caaaactcag acacttaaaa aatttaatca gaacaaataa aaaagcaaac
     5401 aaaaatatcc atctttcaaa ctcatatttt gcaataacct agataagcaa gaacaggggc
     5461 agaacggatt cgcctttgat gtggtggtag gcaaccattc caatatgtcc cagcgacaaa
     5521 aacagcaatg aaaatgccag cgtgccgtga attcctccgc cattgccgat ttgacgggca
     5581 atgcctgcgg cggaacagcg agcatcaggg catacgccca atgcccaatt tggtgacggc
     5641 ggtgttggcg gggcggtttt tacgttggcg gattccctac cctacgcacc aaacagacaa
     5701 tatcagcagc aggattctga ctgcacggat atgttggtat gaatcgtccg cccaagcgga
     5761 aaaggctgtc gcgcccaaaa cagtggcaag aataaattgc ttgaatttca tgatgttgct
     5821 tcttttgcac aaagcgttcg gaatagtgaa gcccgcagcc tatggctttg cggcagtcgc
     5881 agctttacaa gcggtattgc aggaaacctt aaaacacgat cattgcatca agagctgttc
     5941 gaattccgcc aaatgaacct gacacgcaaa ctttgacgct tgaatacggc attcatagct
     6001 tccagtttat cggttcgata ccgtgttgac tcaaatagct gtttgcctgt gaaaaatggc
     6061 ggcagccaaa aaaaccgcga tatgccgaca gaggagacgg atgaggcgcg gtcagtatca
     6121 aatgattttg actgtctatc agcctccctt tttgttgtgc atacccaccc cacaacatga
     6181 agacaaggtg cttgcggtgt gtcgcaagct gcctgatgac ggtatcggta aagcgttccc
     6241 agcctaaaag ggcgtgcgaa tgcgcctgtc ctgcacgcac cgtcaaaacc gtgttcagaa
     6301 gcaatatgcc ctgctccgcc cacgctgtca ggcagccgtg cgcgggaatg gaaaagcctt
     6361 cgatgtcggt ttccaattct ttgtaaatat tgagtaaaga cggcggtatg cggacaccct
     6421 gccggacgga aaatgccaaa ccgtgcgcct gccctacccc gtgatacgga tcttgtccga
     6481 gaatgacgac tttgacccgg tcgaacgctg tcagccggaa tgcgttgaac acatcggcct
     6541 ccggcggata gataatttgc cccgacaaac gttcctgcct gaccgcattt aaaatttcct
     6601 gaaaatacgg ctgctgtttt tcgccgccga gtgcatcgtg ccaagtgtcc atatctgccc
     6661 tatcctttca aattcggcta tactgctgat attgcgttat tttgtcaaac gacgcgcttt
     6721 gtggggaaat gtatgactgt atggtttgtt gccgctgttg ccgtcttaat catcgaatta
     6781 ttgacgggaa cggtttatct tttggttgtc agcgcggctt tggcgggttc gggcattgcc
     6841 tacgggctga ctggcagcac gcctgccgcc gtcttgaccg ccgcactgct ttccgcgctg
     6901 ggcatttggt tcgtacatgc caaaaccgcc gtgggaaaag ttgaaacgga ttcatatcag
     6961 gatttggata ccggaaaata tgccgaaatc ctccgataca caggcggcaa ccgttacgaa
     7021 gttttttatc gcggtacgca ctggcaggcg caaaatacgg ggcaggaagt gtttgaaccg
     7081 ggaacgcgcg ccctcatcgt ccgcaaagaa ggtaaccttc ttatcatcgc aaacccttaa
     7141 cactcggagg aattatggaa tttttcatta tcttgttggc agccgtcgcc gttttcggct
     7201 tcaaatcctt tgtcgtcatc ccccagcagg aagtccacgt tgtcgaaagg ctcgggcgtt
     7261 tccatcgcgc cctgacggcc ggtttgaata ttttgattcc ctttatcgac cgcgtcgcct
     7321 accgccattc gctgaaagaa atccctttag acgtacccag ccaggtctgc atcacgcgcg
     7381 ataatacgca attgactgtt gacggcatca tctatttcca agtaaccgat cccaaactcg
     7441 cctcatacgg ttcgagcaac tacattatgg caattaccca gcttgcccaa acgacgctgc
     7501 gttccgttat cgggcgtatg gagttggaca aaacgtttga agaacgcgac gaaatcaaca
     7561 gtaccgtcgt ctccgccctc gatgaagccg ccggggcttg gggtgtgaaa gtcctccgtt
     7621 acgaaatcaa ggatttggtt ccgccgcaag aaatccttcg cgcaatgcag gcacaaatta
     7681 ccgccgaacg cgaaaaacgc gcccgtattg ccgaatccga aggccgtaaa atcgaacaaa
     7741 tcaaccttgc cagtggtcag cgtgaagccg aaatccaaca atccgaaggc gaggctcagg
     7801 ctgcggtcaa tgcgtccaat gccgagaaaa tcgcccgcat caaccgcgcc aaaggcgaag
     7861 cggaatccct gcgccttgtt gccgaagcca atgccgaagc catccgtcaa attgccgccg
     7921 cccttcaaac ccaaggcggg gcggatgcgg tcaatctgaa gattgcggaa caatacgtag
     7981 ccgcgttcaa caatcttgcc aaagaaagca atacgctgat tatgcccgcc aatgttgccg
     8041 acatcggcag cctgatttct gccggcatga aaattatcga cagcagcaaa accgccaaat
     8101 aagccgtgat gaaaataccg tctgaagctc tgttttcggg tttcagacgg tatttttatt
     8161 taaacccgaa cagggttgta ttccgcttca aatatgtttc ggaaccgtct acctgccccg
     8221 gtaaaaactg ccggcaaaat atttctgcgc gccctcgcga agcaagacca aggttacggc
     8281 ggccaaaggc aatccggcca acattccgac aaagcccatc agctctccga acgccatcag
     8341 cgaaaagata acccaaaacg gcgacaggcc gatacggtct cctacaattt tcggcgtaat
     8401 gaaaaaactt tcgagaaact gaccgacggc aaaaaccgcc caaacagcca agattccgtt
     8461 ccacgaaccg aactggagca aggctgcaac agtggcaagc agcaatcccg taaacgcacc
     8521 caaatagggg acaaacacca aaataccggc aaccataccg atggcaaatc ccgaatccag
     8581 tccgactagc atcaatccca aaccgtaaac caagcccata atcagcatca ccagaagctg
     8641 accgcgcaaa aattcgccca atacctcgtt caaattaccc gtaatgcgcg tataagcacc
     8701 ggcaaaacgc ctcggaacca gtttggcgat gccgcacgac caccgctgcc aatccagcag
     8761 gaaatagtaa agcagcaagg gcggcagcag caggttgccg atactgctga caatattgcc
     8821 gccctgtttc atcaaaacgg gaaaccacgc cttaagcgcg ttgctcaact cgcccgtatg
     8881 cgcctgaaac cacgcaataa tagatgcctg atcgatttcc acatatccgc cgattgtatt
     8941 tttcaaccac ggcagcagcg tgttctgtat aaaaccgatt aattggggca ggcgagatgc
     9001 caaattattg aactgcccga ccagcatagg gacaataatc aacaataatg ccaacaacaa
     9061 aatcaaggaa aacaccatca cagacatcga agcggatgca cggttcaaac cctttttctg
     9121 caaccattcg accaaagggt ccaacacata cgccagcacc gccgcaaccg caaacggagt
     9181 caaagtatcg ccgagcgcgt aaaccagcca gaccaaggcg gcaaacgccg cgccggcacc
     9241 catccacggc ttgatgcccc gtccttttct ccgatacata aaatccaccc ttccgaaaat
     9301 aatattgcgc gccagtatag cagaaccgcc gcgccgtcaa aaaagcctcc gccttcccga
     9361 tagcctgtta tcgtggttta ggctaaaata ctgcacatcc gatgccctct gaaaggcaaa
     9421 cccgccttca gacggcatac cgtacacgat aaggcagcac atcatgaccg aacaaaaaaa
     9481 caaagcatcc ctcatcgaat ttccctgcgc cttcccattg aaagtgatgg gcgcggtgca
     9541 tcccgagttc gagcaggcgg tattggacac cgtccgcctt cacgcccccg acacgcaggc
     9601 gcaccacatc accacgcgtc cgagcagcaa aggcaactat accggtgcca ccgttcaggt
     9661 aaaggttgaa aaccaagaac aattggacaa catctaccgc gcgctgactt cgcacgaact
     9721 ggtcaaagtg gtactttgag atgaaaatca tacacaaagg tttggtcgaa tatctgccga
     9781 cttttgaagc gatgaaaacc tttaatgccg gacggaatgc agacaccgaa gacgaattgt
     9841 gggtcgtcga acacccgccc gtctttacgc aaggactggc gggaaaaccc gagcacctgc
     9901 tgattaggga cgacatcccc gtcgtccaaa tcgaccgggg cgggcagatt acctatcacg
     9961 ggcccgggca attggtcgtt tatacgatga ttgatttcaa acggcgcaaa accagcgtcc
    10021 gcaacatcgt ttccgcgctt gaaaacagca tcatcgccac attggcggaa tacggcatcg
    10081 aagcggcggc agatcccaaa cgccccggca tttacgtcgg cgaacgcaaa atcgcctcac
    10141 tggggctgcg tatcaaaaac ggttccgtct atcacggact cgcactcaac gtcaatatgg
    10201 atttaagccc gtttacccaa atcaacccct gcggctacgc cggtatggaa atgacgcaaa
    10261 tcgcggattt tgtccaaccc tgccccgcgc cggacgaagt cgcctccaaa ctcaccgcac
    10321 accttgaaac acaactcaca ccgaaagcag acaacaatga gtgaaatcaa aaccgacgac
    10381 cccaaacgcg gcatcaaact cagaggcgcg gacaaaaccg cccgcatccc catcaaagtc
    10441 gtcccccttc aggaaaaact gaaaaagccc gaatggatac gcgccaaact cccatcgcgc
    10501 aaattctttg aaatcaaaga cattttgcgc gaacaaaaga tgcacaccgt ttgcgaggaa
    10561 gcctcctgcc ccaacatcgg cgaatgcttc agcaaaggca cggcgacctt catgattatg
    10621 ggcgacatct gcacccgccg ctgcccgttc tgcgacgtgg gacacggccg tccgaatatg
    10681 ctcgaccccg acgagccgaa aaacctcgca gaatccgtca aagccatgaa cctgcgttac
    10741 gtcgtcatca cctcggttga ccgcgacgac ctgcgcgacg gcggcgccca acacttcgcc
    10801 gactgcatca aagccatccg cgaaaccagc ccgaacacca aaatcgaaat cctcgtcccc
    10861 gacttccgag gccgcttgga catcgcactg aaaatccttg ccgaaacccc gcccgacgtg
    10921 atgaaccaca acttggaaac ccatccgagc ctgtacagaa aagcccgtcc cggtgccaat
    10981 tatcaacatt ctttagactt attaaaacgt tataaagaaa tgatgccgca tatcccgacc
    11041 aaatccggca ttatggtagg cttgggcgaa acagacgaag acgtgcgtga aattatgcgc
    11101 gatatgcggg cgcacaatat cgagatgatt accatcggac agtacctcca gccttcagac
    11161 ggacacttgc ctgtcttgcg ctacgttaca cccgaacaat tcaaaatatt tgaaaaagaa
    11221 gcatacgaac tgggctttac caatgccgcc atcggcgcga tggtacgttc aagttaccat
    11281 gccgacgaac aggcggcgga agccttacgc gaaagccacg gcggttgcgg gcatcattaa
    11341 actcggaaca ccgttccgaa gtaaaaaata atttcctacg ctgctgtctg ccgccttgtt
    11401 tgatgtaaac aacatcaatt tgaattgtcc atgccgtctg aaagtccttc agacggcata
    11461 atttattccc aaaatccgat aatctcaaaa gaatctgtcg gttaatgaga aagattcatt
    11521 ataaattttg ataaaaaacc gtgctctatt tgttttttgc tatggttaac aagtgtaaat
    11581 gttctttatt gatgagatgg attcttagtt ttagcagcaa aaaaaaccga taagaaacag
    11641 catactgatt tggaaggcat cggggaatac gccctattta ttttgataat tgtttgtatt
    11701 ttaaatagaa aatttgtcat gccgacagct tttgcataca atgcacgcat cccgaacagc
    11761 gttacagaac gccgtttaac cgcacgaaac ccaaattaag aaaagagaga tattatgcaa
    11821 ggcaatcaag ctgttgttga ttacatgaac gaattgctgt ccggcgagct ggcggcacgc
    11881 gaccaatact tcatccactc ccgcctctac tccgaatggg gctacaccaa actttttgaa
    11941 cgtctcaatc acgagatgga agaagaaacc acacacgccg aagacttcat ccgacgcatc
    12001 ctgatgctgg gcggtacgcc gaaaatggca cgctccgaac tgaatatcgg cacggacgtg
    12061 gtttcctgcc tcaaagccga cctgcaaacc gaatacgaag tgcgcgacgc tttgaaaaaa
    12121 ggcatcaaac tgtgcgaaga ggctcaagac tatgttacgc gcgacctgat ggttgcccaa
    12181 ctgaaagaca ccgaagaaga ccacgcacac tggttggaac agcagctgcg cctgatcgag
    12241 ctgattggcg aaggcaacta ctaccaaagc caactgtaat ccgtattagg aataaaagga
    12301 gtccgtatga aaggcgaccg tttggttatc cgcgagctga acaaaaactt aggcttgctg
    12361 ctggtaacca ttaaccaata tttccttcac gcccgtattt tgaaaaactg gggctttgaa
    12421 gaactgggcg aacatttctt caaacaatca atcgtagaaa tgaaagctgc cgacgatttg
    12481 attgaacgca tcctcttcct tgagggattg cccaatttgc aagagctggc caagctgttg
    12541 attggtgaat ctaccgaaga aatcatcgcc tgcgatttga ccaaagaaca ggaaaaacac
    12601 gaagccctgc ttgccgccat cgccacagca gaagcgcaac aggattatgt cagccgtgat
    12661 ttgttggaaa aacaaaaaga tactaacgaa gaacacatcg actggctcga aacccagcag
    12721 gaactgatcg gcaaaatcgg tttgccgaac tacctgcaaa cagcggcgca agaggactaa
    12781 aacacaaacc actgccaaat acagcagtcc cctgcatcat cagacggcat atccgtataa
    12841 acgggtatgc cgtttttctt tgccggttta cgaataaaat ctaaattgat ttgaatcaat
    12901 actcgataac ggtttttgtt tgataatacc gatatgaaat ctagattgtt tgtttgctgt
    12961 ttcccaatcc gtcttgattt tacctctttc ctcttgatgt gtgtgtgtgt ttgggtgtgg
    13021 ctgccgccac cccttttttt tggcttttat gtgaagtaaa atccgtaaca gcaaatactt
    13081 aacttttaac catttatccg gaaacaaatg atgggcaaca aattgactct gcctgccgaa
    13141 ttgcctgacg aacaggattt gcgggcggta ttggcataca atatgcggct tttccgcgtg
    13201 aacaagggtt ggtcgcaaga ggaattggcg cggcaatgcg gtttggacag gacttatgtg
    13261 tcggcagtcg agcgcaaacg ctggaacatt gccctgtcga atatcgaaaa aatggcggcg
    13321 gctttgggcg tggcggcgta tcagttgctg ctgccgccgc aggaacggtt gaacctgatg
    13381 accaattccg ccgatacccg acaagtgccg tctgaaagcg gtatttgacc gccgaaccat
    13441 ccgcctccct ttttcccgcc aatcgaaaga ataaaaatgc ctgaaaacct gtcttcagca
    13501 ctggaaacct tcaaacagca gcgcaatgcc gccgaagcgc attatttgaa agccaaccgc
    13561 gtgtcggtat ttttcagaga atacacggcg gcagtcgaaa ccttgctggc ggcattgtgg
    13621 gcagaacatt ttcaaaacag cgcgttatgc ctgatggcgg taggcggctt cgggcgcggc
    13681 gaaccgtatc cctgttcgga tgtggatttg gcggttgtct cccctgtccc gctttcagac
    13741 ggcattcagg aacagattgc ccgatttatc caaactctgt gggattgcaa actaatgccg
    13801 tctgtaaaaa gcggcagcgt tgacgaactg tgcgaaagcg tgcgcgacga tattacgggg
    13861 gacacagcgt ttttagaggc taggtttttg tttggcaacc gccaaacagc agacgaattg
    13921 gcggaaaaaa tgaacgtgca acgcaatgta gcggcattta tcgaagcaaa attggtggag
    13981 atggaacacc gccacgccaa atcgcaaggt tcgggggcgg tgttggagcc gaatatcaaa
    14041 agctgtccgg gcggtctgcg cgacatccac accctgcttt ggatagcgaa ggcgcaaggc
    14101 ttggcggcca acctgcccga cctgctcaaa cagcggattt tgacacgtgc cgaagccggt
    14161 atgctttcgc acggctaccg ccgcctcgcc cacatccgca tccggctgca tttaaacgcc
    14221 aagcgcgccg aagaccgcct gcttttcgat ttgcagccgc aagttgccga aagtatgggt
    14281 tatcaagatg aaaaccgccg ccgtcaaagc gaagaactga tgcgcgtgtt ttaccgtgcg
    14341 gtcaaaaccg tcaaacaact gggcggcatc ctcacgccca tgctgcgaag ccgcgtttcc
    14401 tccacgccgg tgcgcgttac cctgcggatt gacgacgact acatccaagt caacaaccaa
    14461 atcgccgcgc ggcacaccga tatttttttc agacggcccg aacacatttt caaaatcgtc
    14521 gaaatcatgc agcagcgcaa cgacattact gcgctcgaac cgcaaaccct gcgcgcatgg
    14581 tggggggcga cgcgcaaaat caaccgcagc ttctaccaaa attctgaaaa ccgtcgccgc
    14641 ttcgccggtt ttttccgcag cggcaacgga ctgacccaaa ccctgcgctt tctcaacctc
    14701 tacggcgtgt tgggccgcta tctgcccgcg tgggaaaaaa tcgtcggcct gcttcaacac
    14761 gacttgttcc acatctatcc cgtggacgac cacatccttg ccgtcgtccg caacgtccgc
    14821 cgccttgccc tagatatgca cagccatgag ctgccctacg cctccgcgct gatgcagtcc
    14881 tttgaaaaac aagacatcct ctaccttgcc gccttcttcc acgacatcgc caaaggacgc
    14941 ggcggcgacc atgccgtaca aggcatcgca gacgcgcgcc aatttgccgc cgaccacttc
    15001 ctgaccgaag aagaaagcga cctgctcgcc tggctggttg aaaaccacct ccttatgtct
    15061 gccgtcgccc aaaaagaaga catccaagac cccggcgtac tcgatgcctt ctgcaaacgc
    15121 gtccaaaccc acgaacgcct cagcgcgctc tacctcctga ccatttccga catacgcggc
    15181 accaatccca agctgtggaa cgcctggcgc gccagcctgc tggaaagcct gttccacgcc
    15241 gccggacgct gcctcgcagg caacgacggc aacccgcacg ccctcttcgg ccgccgccgg
    15301 caggaagccg ccgacttact tacccgcgcc gccgtccccg aaaaacagca gaaaaaacta
    15361 tggaacgcgc tcggttccgc ctatttcgcc cgccatcagt cccgcgaaat cctgtggcac
    15421 gccgccaacc tcgtacacga ctttgaaccc cccatcgtcc gcagccgcat cctaccccaa
    15481 agcgacagct ttcaagtgat ggtctttatg cccaacggcc cgcgcctgtt cgcccgcctc
    15541 tgccgcatct tcagccgcca cggcttcgat atcctcgccg cccgcgcctt tatcaccgaa
    15601 cacgactaca tcctcgacac cttcatcgtg caaatcccct cgcagcacgc ccccgaagac
    15661 taccccgaca tccaaagcgc gctcgaagcc gaactcaaca gctttatcca cggacacacc
    15721 gttgccgaaa cccaaagctg caaccgccgc atcagccgcc gcagccgcta tatgcctatt
    15781 gcgccaagca tcaccatcac ccccgaagaa gactatccgg accggtattc cgtcgaaatc
    15841 accgccgtca accgcccctt cctgctcgcc gacatggcgg aagtcttctt cgcccacaac
    15901 gtcagcctgc gctacgccaa aatctccacg ttggacgaac gcgtcgaaga cagctttacc
    15961 gtcttcagcc ccgacttgaa aaacccaaaa atccagtcct cattgaaaca ggctttgctg
    16021 gaacagttgg cataagcacc ctgccggttt tcaaacggca tcccgccgcc acgccgtatg
    16081 ccgtctgaag gcgggggata ttgcaaacaa tataaactgt caagccgccg gcaacgtgct
    16141 ataatgccgt gcatcttgca caaaccgaaa gaccgagcat tatgcgtatc gtagaaaaag
    16201 cctatacttt cgacgatgtt ttgttggttc ccgcacattc gaccgtgctg ccgcgagacg
    16261 ttaaacttca aaccaagctt acccgcgaaa tcacactcaa cctccccctg ctttccgccg
    16321 cgatggatac cgttaccgag gcgcgcctcg ccatttcgat ggcacaggaa ggcggcatcg
    16381 gcatcatcca taaaaacatg ccgcccgaaa tgcaggcgcg cgccgtttcc aaagtaaaac
    16441 gccacgaaag cggcgtggtc aaagaccccg taaccgttgc gccgacaacg ctcatccgcg
    16501 aagtcttgga aatgcgcgcg cagcgcaaac gcaaaatgtc cggcctgccc gtcgttgaaa
    16561 acggcaaagt cgtcggcatc gtaaccaacc gcgacctgcg ttttgaaaac cgcgtcgatt
    16621 tgcccgtttc cgccattatg accccgcgcg aacgtctggt taccgtcccc gaaggcacaa
    16681 gcatagacga agcgcgcgaa ctgatgcaca cgtacaaagt cgaacgcgtt ttggtcctga
    16741 acgaaaaaga cgagcttaaa ggcttgatta ccgttaaaga tattttaaaa accaccgagt
    16801 ttcccaatgc caacaaagac tccgaaggcc gtctgcgcgt cggtgcggca gtcggcaccg
    16861 gcggcgacac cgacgagcgc gtcaaagcct tggtcgaagc cggcgcggac gtgattgtcg
    16921 tcgatactgc ccacggtcat agccaaggcg tgatcgaccg cgtgcgttgg gtcaaagaaa
    16981 cctatccgca catccaagtc atcggcggca acatcgccac tgccaaagcc gctttggatt
    17041 tggttgccgc cggcgcggat gccgtcaaag tcggtatcgg tccgggttcg atttgcacca
    17101 cccgtatcgt ggcaggcgtg ggtgtgccgc aactgaccgc cattcacaac gttgccgaag
    17161 ccctcaaagg cacgggcgtt ccgctgattg ccgacggcgg catccgcttc tccggcgaca
    17221 tcgccaaagc cctcgccgca ggcgcgtaca gcgtcatgct cggcggtatg tttgccggca
    17281 cggaagaagc cccgggcgaa atcgaactct accaaggccg ctcgtacaaa tcctatcgcg
    17341 gtatgggttc cttgggcgcg atgagccaag gttctgccga ccgctacttc caagacaaaa
    17401 ccgacagcac cgacaaatac gtccccgaag gcatcgaagg ccgcgttccc tacaaaggcc
    17461 cgattgtgaa catcatccac caactgaccg gcggtttgcg ttccagtatg ggttatttgg
    17521 gttgcgccaa tattgccgaa atgcacgaaa aagccgaatt tgtggaaatc acttccgcag
    17581 gtatgagcga atcgcacgtt cacgacgttc aaattaccaa agaagcgccg aattaccacc
    17641 gctgatttga acagcctttt caaggcaaaa tgccgtctga aacctaattt ccgtgtttca
    17701 gacggcattt tttgtccgtt caaggcaagc cgcacgggct gattgcgcgg acagaaaaag
    17761 ccattctgac ggacacgccg aaagaatgaa aatgccgtct gaacgccgtt tgcaccccgt
    17821 ctgccgaata ccgtcggcct ttaaaacggc ggtggaaata aacgcaatct ttcaatacgg
    17881 gcgtttcgat tggtcggaaa tgatgcgccg gccgccatat caaaaaatcg ggcggccgct
    17941 tcgggaaagt atgccccg
//
LOCUS       DAWTHD010000037        17764 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWTHD010000037 DAWTHD010000000
VERSION     DAWTHD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17764
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.037"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="WM704_000960"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="WM704_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFA9340405.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(414..596)
                     /locus_tag="WM704_000961"
     CDS             complement(414..596)
                     /locus_tag="WM704_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340406.1"
                     /translation="MDASAQTKPDSTFFRGASLRKVMDKKYPVLFTKKCRLKILEAQN
                     KPISPTALFFDFVVLP"
     gene            complement(670..783)
                     /locus_tag="WM704_000962"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="WM704_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="WM704_000963"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="WM704_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFA9340407.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="WM704_000964"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="WM704_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFA9340408.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="WM704_000965"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="WM704_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFA9340409.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="WM704_000966"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="WM704_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFA9340410.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="WM704_000967"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="WM704_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFA9340411.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="WM704_000968"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="WM704_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFA9340412.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="WM704_000969"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="WM704_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFA9340413.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="WM704_000970"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="WM704_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFA9340414.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="WM704_000971"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="WM704_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFA9340415.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="WM704_000972"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="WM704_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFA9340416.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="WM704_000973"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="WM704_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFA9340417.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="WM704_000974"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="WM704_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFA9340418.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="WM704_000975"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="WM704_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFA9340419.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="WM704_000976"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="WM704_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFA9340420.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="WM704_000977"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="WM704_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFA9340421.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="WM704_000978"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="WM704_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFA9340422.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="WM704_000979"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="WM704_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFA9340423.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="WM704_000980"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="WM704_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFA9340424.1"
                     /translation="MAKHEIEERGNGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="WM704_000981"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="WM704_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFA9340425.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="WM704_000982"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="WM704_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFA9340426.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="WM704_000983"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="WM704_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFA9340427.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="WM704_000984"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="WM704_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFA9340428.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="WM704_000985"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="WM704_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFA9340429.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="WM704_000986"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="WM704_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFA9340430.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="WM704_000987"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="WM704_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFA9340431.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="WM704_000988"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="WM704_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFA9340432.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="WM704_000989"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="WM704_000989"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFA9340433.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="WM704_000990"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="WM704_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFA9340434.1"
                     /translation="MRYRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="WM704_000991"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="WM704_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFA9340435.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="WM704_000992"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="WM704_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFA9340436.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="WM704_000993"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="WM704_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFA9340437.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="WM704_000994"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="WM704_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFA9340438.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4889 a         3366 c         4386 g         5123 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtcaagc ttggtacatt tttgccaagt ttaactttgt ctgaaagaca ggccaatcgc
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa
     1141 gttggtatga cccgcgtgtt cgacgagcag ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagata ctgacggcta taccgccgtt
     1261 caagttacct ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt cggtcaactg
     1441 gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg gcacgatcaa acgtcataac
     1501 ttcggtgccc aacgtacttc ccacggtaac tcccgttctc accgcgttcc aggctctatc
     1561 ggtatggcgc aagacccggg tcgcgtgttc cccggtaagc gcatggccgg ccaatacggc
     1621 aacaccaaag caactgttca aaaattggaa gttgtccgtg ttgatgcaga acgccaactg
     1681 ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcccagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcaccagc
     1861 tggtaaatgc ctacttggca aacgcccgct ctggtaaccg tgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgc gcccgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc cggtatggcg actatcctgt
     2101 cccaattggc gcgtgacgag cgtttgtttg tgattgaggc gttgactgcc gaaactccca
     2161 aaaccaaagt ttttgccgaa caagtaaaaa atttggctct ggagcaagtg ctgtttgtaa
     2221 ccaaacggct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtattggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta taaaaaagta atcatcacta
     2341 aagatgcggt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 aatcttggcg cctgtcgttt ctgaaaaaag caacgtattg gctgaaaaat gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg tcgagctgct
     2521 gttcggtgtt caagttgctt ctgtaactac cgttaccact aaaggcaaaa ctaagcgttt
     2581 tggtcgtatt ttgggtcgcc gcagcgatgt taaaaaagct tatgtaagct tggctgacgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcacc ttatgcaccc ttgcttgaaa agaaaaattc tactgccggc
     2821 cgtaacaata atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggcatttctg ctaaagtaga gcgtatcgaa
     2941 tacgatccta accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgtcgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagtctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gacggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagc tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtctttcag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtaa cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgcgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg cgggtagtca aaatacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctctttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggc cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctact ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgttatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatta tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggtg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggcatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg tgaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctctttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttccga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga taaaacgctt
    17581 gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca ggtttccgta
    17641 tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat
    17701 caggtaaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg gaatgacggc
    17761 atat
//
LOCUS       DAWTHD010000038        17424 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWTHD010000038 DAWTHD010000000
VERSION     DAWTHD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.038"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(394..1236)
                     /locus_tag="WM704_000995"
     CDS             complement(394..1236)
                     /locus_tag="WM704_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9340439.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1471..2391
                     /gene="pyrB"
                     /locus_tag="WM704_000996"
     CDS             1471..2391
                     /gene="pyrB"
                     /locus_tag="WM704_000996"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFA9340440.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2401..2859
                     /locus_tag="WM704_000997"
     CDS             2401..2859
                     /locus_tag="WM704_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFA9340441.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            2921..3586
                     /locus_tag="WM704_000998"
                     /pseudo
     CDS             2921..3586
                     /locus_tag="WM704_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3991..5214)
                     /locus_tag="WM704_000999"
     CDS             complement(3991..5214)
                     /locus_tag="WM704_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9340442.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     GISGKYLYSPWQWCRLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KISPDKEVSGYGIPAIDVNFYRVFMQHPQIVSRKETAAAPRLLSGPEGRLLYTKDARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
     gene            5398..5553
                     /locus_tag="WM704_001000"
     CDS             5398..5553
                     /locus_tag="WM704_001000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340443.1"
                     /translation="MALLNIEQGYNTTLHNRSPYLINFGLSCLIGLEKAAAAPRDVPR
                     DNKCSAF"
     gene            5584..6129
                     /gene="def"
                     /locus_tag="WM704_001001"
     CDS             5584..6129
                     /gene="def"
                     /locus_tag="WM704_001001"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFA9340444.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LVADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDAVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            6217..7143
                     /locus_tag="WM704_001002"
     CDS             6217..7143
                     /locus_tag="WM704_001002"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFA9340445.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            7222..8481
                     /gene="rsmB"
                     /locus_tag="WM704_001003"
     CDS             7222..8481
                     /gene="rsmB"
                     /locus_tag="WM704_001003"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFA9340446.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            8447..9046
                     /locus_tag="WM704_001004"
     CDS             8447..9046
                     /locus_tag="WM704_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFA9340447.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            9046..11166
                     /locus_tag="WM704_001005"
     CDS             9046..11166
                     /locus_tag="WM704_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFA9340448.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            11159..12436
                     /locus_tag="WM704_001006"
     CDS             11159..12436
                     /locus_tag="WM704_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFA9340449.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            12527..13714
                     /gene="dprA"
                     /locus_tag="WM704_001007"
     CDS             12527..13714
                     /gene="dprA"
                     /locus_tag="WM704_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFA9340450.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            13740..14201
                     /locus_tag="WM704_001008"
     CDS             13740..14201
                     /locus_tag="WM704_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFA9340451.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            14279..16585
                     /gene="topA"
                     /locus_tag="WM704_001009"
     CDS             14279..16585
                     /gene="topA"
                     /locus_tag="WM704_001009"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFA9340452.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPGNGFAMKYQLISRNAKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPDEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIELPAPQE"
     gene            16833..17424
                     /locus_tag="WM704_001010"
                     /pseudo
     CDS             16833..17424
                     /locus_tag="WM704_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         4125 a         4707 c         5042 g         3550 t
ORIGIN      
        1 aaatagtacg aaaccgattc acttggtgct tcagcacctt agggaatcgt tctctttgag
       61 ctaaggcggg gcaacgccgt actggttttt gttaatccac tataaatagc gagcatcgat
      121 tcacggtgcg ctttagtgca aagcgtacaa cgcgagcctg aaccaccagc cgcaatagga
      181 aaagaaagcc gatagagtgc aatgcttgcc aacgcgcaag cgagcttgca agaacgcttg
      241 gctcaacgag agcaggcaag acagaaagca ggataggaga ggcaacgcaa aggtctcggg
      301 ctttgatttc gccgtaaacc tgagtgccgc cttgtccgga cggggtggtt caggcggcgg
      361 gtgccgacag ggtgcagatg aggaggagta ttttcatttg gggtcgcaac ggaagtggta
      421 ggcgcagatt tcaaaaccgt ttttgaaata caggcggtgc gcgtcggtac ggtcgtggtt
      481 gacgtggacg ttaaggtgga ttttggtaac ccctgtttcc gcgctgattt tgcggacttc
      541 ttccaaaagg cgcgaggcgt agcctttgcg gcggctttgc ggcagggtaa cgatgtcgtc
      601 gatgtggatg tggcggccgc cggcgagggt gcaggcttcg cggaagccgc agacagcgac
      661 ggcgttgtgt ttgccttctt caaaaatgcc cagcaggcgg tagccttggg ggcgttgggt
      721 tttgttgatc tgttcggtaa agcggccgat gtcggtcagg gcggaacgca aaacactcaa
      781 ggctgcaaag gcggtggcgg tgtcgtccgc gccgatttcg cgtaaaacgt aggatgcgcc
      841 cgaggcggtc tgttcctgtg ttttctcggc ggcgtgtttt tcttcgattg cctgtgccag
      901 caggacgcgt tcgttggcag ggtcgttttg tccgccctgt tcgcgttctt cgagcagggc
      961 tttgcagtcg atgacgcgca ggtcgttgtc ggcggcaaag tccatcagga agcggaacat
     1021 ctgcggattg tcggtttcca gttttttatc gacggcgacg cagcggatgt tgtccaccag
     1081 catggggcgc acccatttcg aataggagag gcggttgtct ttggtgaacg aggacaggat
     1141 gccgcacagg cgttccgccc agtcgccggg gcggaaaatc ttgccggaac tcgttgtgcc
     1201 gtggatgacg acttcgtagg ggttgcagat taacatggcg gcttcctgaa aagaaatgtc
     1261 tggcgcgatt ataccttatg cttatgcggg cgtgtttgga tatgccgtct gaaaaataca
     1321 ggattcgtgc ggtaaaactt tgtggaggca aatgtgcgat aatacgcgcc gtgtcgccgc
     1381 ttttgcgaag ctgttccgca aacatacggg cggcgtggac gtataaccgg atgcccgcct
     1441 gacgcgggtt ttttacggaa ggggggcaaa atgcctaatc cgctttacag acagcacatc
     1501 atctccattt cggatttgtc gcgcgaacag ttggaatgcc tgcttcagac ggcattgaag
     1561 ctgaaggcgc atccgcgcgg cgatttgttg gaaggcaaac tcatcggttc gtgctttttc
     1621 gagccgtcca cgcgcacgag gctgtcgttt gaaacggcgg tgcagcgttt gggcggtaag
     1681 gtcatcggct tttcggacgg cgcgaatacc agtgccaaaa aaggcgaaac gcttgccgat
     1741 accgcccgta tcatttccgg ctacaccgac gcgattgtcc aacgccatcc caaagacggc
     1801 gcggcgcgcg tggcggcgga gttttcgcgc gtccccgtca tcaacgccgg cgacggcacg
     1861 aaccagcacc ccagccaaac cttgttggac ctggtaacga tttatgaaac gcaggggcgt
     1921 ttggacaagc ttaaaatcgc catggcgggc gacttgaaat acgggcgcac cgtgcattcg
     1981 ctgtgtcagg cgttgaaacg ctggggctgc gaatttgcct tcgtctcgcc gcccagcctc
     2041 gcgatgcccg actacattac cgaagagttg gaagaagccg actgccgata ccgcgccctc
     2101 ggcagtttgg aagaggcggc ggaatgggcg gacatcctgt atatgacccg cgtccagcgc
     2161 gaacgtttcg acgaacagga atttgccaaa atccaaggca aattcaacct cgaagcgtct
     2221 atgctcgccc gcgccaaacc gaacctgcgc gtgctgcacc ccctgccgcg cacggacgaa
     2281 atccaccccg atgtcgatgc cgcgccgcac gcctattatt tcgagcaggc gaccaacggc
     2341 gtttatgcgc gtatggcgat attgtcgctg gtgttgaacg aagaagtgtg aggaaccgat
     2401 atggaagcgc aaaaactcag tgtcgaagcc atcgaaaaag gcacggttat cgaccatatt
     2461 cccgccggca gggggctgac catcctgcgc cagttcaaac ttttgcacta cggcaacgcg
     2521 gtaaccgtgg gcttcaacct gcccagcaaa acccagggca gcaaagacat catcaaaatc
     2581 aaaggcgtgt gcttggacga taaagccgcc gaccgcctcg ccctgttcgc ccccgaagcg
     2641 gtggtcaaca ccatcgacaa tttcaaggtc gtacagaagc ggcatttgac cctgcccgac
     2701 gaaatcgccg aagtgttccg ctgcccgaat cccaattgcg ccggccacgg cgagccggtc
     2761 aaaagccggt tttatgttaa gaaacacaac gggcagacgc ggctgaaatg ccactactgc
     2821 gaaaaaacct acaaccggga ttcggtggcg gaagcctgac ggattcccga gcccggcggc
     2881 ggaattttgc ctgccgcctg ttttgccaat ctgaaatgga atgatgatgc acgcttctgt
     2941 ccaaagtcgt ttcgcaccga tactttatgt tttgattttc tttgccggtt ttttgaccgc
     3001 gcaaatctgg ttcaatcaga aagcctatac tgaagagctg cctccgcttc tgtccgcatt
     3061 gtccgccgtc gcgctggtgt ggctggcgtg ggcgttcgtg tcggtgcgtt caaaggctaa
     3121 ggcagaaaag ttctaccgcg aaaaaatgat acagaacgaa agcatacccc cgtcctgcac
     3181 gcttctttgc aacacttgga acacaagccg caaatgctcg ccctgctggt caaaaaccac
     3241 ggcaaaggca tggcggaaca ggtcaggttc aaggcggaag tgctgcccga cgacgaagac
     3301 gcgcgcacga ttgccgccga gttggcaaaa atggatatgt tcgcattggg gacggacgcg
     3361 gtcgcctcgg gcgaaaccta tgggcgcgtg ttcgccgata ttttcgagtt gtcggcggct
     3421 ttggaagggc gcgcgttcaa agggatactg aaactgacgg cggaatataa aaaacatctt
     3481 cggcgatgcc tgccgttcgg aaacggcgtt ggatttgggc gcgctcaatc aggcgttgag
     3541 ggaaatctcg aaaacgccgg aaaagcctaa gcagatattt tattgaaggt gtcccgatgt
     3601 atggggagcg cacgggtggc gcggcggcaa agcggcaaga ttgggtttgg atacccggtt
     3661 ggttttatag tagattaaca aaaatcagga caaggcggcg ggccgcagac agtacaaata
     3721 gtacggaatc ggttcgcccg gtgcttgggc accttaggga atcgttccct ttgagccggg
     3781 gcggggcaac gccgtaccgg tttttgttaa ttcgctatat ctgtctgcaa gggagacagc
     3841 cgctgtgatt tccgttcggg cggaaaccga ttccatcgga taaaaggcat tttgtccgac
     3901 taaatcaggg ttttgcatca acgggatatg aaaacacagc ctaaataaaa atgccgtctg
     3961 aatgcttcag acggcattgt tgccgagggg ttattggaaa gggtcggaat cgacgcgggc
     4021 gcggccctga tccggcatat tgtccaaatc ccgtcccgga ttggcggcgg tgtcgccttc
     4081 ggaaatatcg gagatgtttt ccaaaataat ggcgtaagcc agtttcggcg cagtgtggta
     4141 aaccattgcc aagcccactt cttcggcagg tgtcgaaatc agctcgacgg tatccctgct
     4201 tttaggttct tcggtcaaat tgttggagag gttgacctgc atcgttttct tgcgtttgta
     4261 aaggcttaaa actgcgcctt tgtccaaacc gtcgtccccg cctttgtcta tcgtaatggt
     4321 tttgaactgt ccgccgaccc ccacgccttc aaacacggaa acgatttttg cctgaaccgg
     4381 gcgggacggt tcgtgcggca ccatattgaa gcggtcggtg tcttccggca ttttcatcag
     4441 gtagtcgccc tgctgtattt cggaaatggc ggtttcgacc accagcggct gtatcgaacg
     4501 ggtgcgcacc ggggtaatca aaggatgggt gcgggtgtag tattcgttgt ctttcagccg
     4561 ttcttcagcc tgtttcgagc gttgttcgag ggcggagtcg gtatagtcga gggagcgcac
     4621 gatgccgctg aatgcgactt cttgtccgag gaatttaccc gtatccggat cggtgatgtt
     4681 tttattgatt cggtaggtca gataacggcc cggctctttc aggcctttgg tgtaaaccct
     4741 ggcgtctttg gtgtacagca gcctgccttc cgggcccgag agcaggcgcg gcgcggcagc
     4801 ggtttctttg cgggaaacga tttgcgggtg ctgcataaag acgcggtaga agttgacatc
     4861 gatggcggga ataccgtatc cggacacttc cttatccgga ctgattttga cgacggggat
     4921 gccgtctgtc tgttccaaat caaggcgcgg ttcgccgcca acgtagcgca acaccaatac
     4981 ctggcccgga taaatcaggt cgggattgtg gatttgatcc cggttcgcgc cccacaggcg
     5041 gcaccattgc cacgggctgt acaggtattt gcccgaaata ccccacaggg tgtcgccctg
     5101 tttgaccgtg tagcgttgcg gcgcatccgg gcgcacctcc agatttgccg ccaaagtttg
     5161 tgttgagaat gccatacctg ccgcgcagag cagggttata atacgacgtt gcataaccgt
     5221 tccccttatc tgataaattt cggtttgtct tgcttgattg ggttggaaaa agcggcggca
     5281 gcccctcggg atgtgccgca tgataacaaa tgttccgcat tttaacatcg aattatccgt
     5341 accatcacgg taattatgaa aaacaggcgg cgtatccgcc gaaggaaaga gaaaattatg
     5401 gctttactga atatagagca gggttataat acgacgttgc ataaccgttc cccttatctg
     5461 ataaatttcg gtttgtcttg cttgattggg ttggaaaaag cggcggcagc ccctcgggat
     5521 gtgccgcgtg ataacaaatg ttccgcattt taacatcgaa ttatccgtac catcacggta
     5581 attatgaaaa acaggcggcg tatccgccga aggaaagaga aaattatggc tttactgaat
     5641 atcttgcaat atcccgacga gcgtctgcac acggtggcaa agcctgtcga acaagttgac
     5701 gagcgcatcc ggaagctggt tgccgatatg tttgaaacga tgtacgaatc gcgcggcatc
     5761 gggctggcgg cgacgcaggt cgatgtgcac gaacgcgtgg tcgtgatgga tttgaccgaa
     5821 gaccgcagcg aaccgcgcgt gttcatcaac cccgtcatcg ttgaaaaaga cggcgaaacc
     5881 acttacgaag agggctgcct gtccgtaccg ggcatttacg acgccgttac ccgcgccgaa
     5941 cgcgtcaagg tcgaggcttt gaacgaaaaa ggcgaaaaat tcacgctgga ggcggacggg
     6001 ctgctggcga tttgcgtgca gcacgagtta gatcacctga tgggcatcgt gtttgtcgaa
     6061 cgcctttccc aactcaagca ggggcggatt aagaccaaac tgaaaaaacg tcagaaacat
     6121 acgatttgat tcgcttcgtc atgccgtctg aacgctgcaa ggctttcaga cggcacagcc
     6181 ttgtccgaca actttacgca cacgcaggaa cacgctatga aagtcatctt tgccggcacg
     6241 cccgattttg ccgccgccgc cttaaaagcc gttgccgccg ccggttttga aatcccgctg
     6301 gtgctgaccc agcccgaccg tccgaaaggg cgcggtatgc aactgaccgc cccgcctgtc
     6361 aaacaagccg cgctggaact cggtttgcgc gtggcacagc ccgaaaaact gcgcaacaac
     6421 gccgaagccc tgcaaatgct caaagaggtc gaggcggacg taatggtggt tgccgcgtac
     6481 ggcttgattc tgccgcagga cgtgttggat acgccgaaac acggctgcct caatatccat
     6541 gcttcgctgc tgccccgttg gcgcggcgcg gcgccgattc agcgcgcgat tgaggccggc
     6601 gatgccgaaa ccggcgtgtg cattatgcag atggacatcg gtttggacac cggcgatgtg
     6661 gtcagcgaac accgctacgc catccaacct accgatactg ccaacgaagt ccacgacgcg
     6721 ctgatggaaa tcggtgcggc ggcggttgtt gccgatttgc aacagcttca aagcaaaggc
     6781 cgtctgaacg cggtcaaaca gcccgaagaa ggcgtaactt acgcccaaaa actaagcaaa
     6841 gaagaagcgc gtatcgattg gagcgaaagc gcggacatta tcgaacgcaa aatccgcgcc
     6901 ttcaaccccg tgcctgccgc gtgggtcgaa tatcagggca agccgatgaa aatccggcgt
     6961 gccgaagtgg tggcgcaaca aggtacggca ggcgaagtgt tgtcctgttc ggcggacggt
     7021 ttggtcgttg cctgcggcga aagcgcgctg aagattaccg aattgcagcc tgccggcggc
     7081 aggcgtatga atattgcggc gtttgcggcg ggacggtcta ttgaagcagg ggcgaagctg
     7141 taaatccctt cagacggcat tccgatccgc aaacgggaat gccgtctgaa accatcagtt
     7201 gaagaaagcg aatcacataa tatgagtatg gcacttgccc aaaaacttgc cgccgacagc
     7261 attgcggcgg ttgccgaagg gcgcaacctt caggacgtgt tggcgcacat ccgcgcggcg
     7321 catccgcagc tgacggcgca ggaaaacggc gcgttgcagg atatcgccta cggctgccag
     7381 cgttatttgg gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc catcgacaat
     7441 ccgcagctcg aaagcctgct tttggcggcg ttgtaccaac tgcattacac gcgcaacgcg
     7501 ccccacgccg tggtcaacga agctgttgaa agcatagcca aaatcgggcg cgggcagtac
     7561 cgttcgtttg ccaacgcggt tttgcgccgc tttttgcgcg aacgtgaaaa attggcggct
     7621 tcctgcaaaa aagacgatgt ggcgaaatac aacctgccgc tgtggtgggt tgcctacctg
     7681 aaaaaccatt atccgaaaca ctggcacaac atcgccgccg cgctgcaatc ccatccgccg
     7741 atgacgttgc gcgtcaaccg ccggcacggc aacgccgaaa gctatttgga aaaactggct
     7801 gtggaaggta tcgcggctaa ggcgttggac gaatatgcgg ttacgttgga agaagccgtg
     7861 ccggtaaacc gtctgcccgg ttttgccgaa ggactggtgt cggtacagga cttcggcgcg
     7921 cagcaggcgg cgtatctcct taacccgaaa gacggcgaac ggattttgga cgcgtgcgcc
     7981 gcgccgggcg gcaagacggg gcatattttg gaactggcgg actgccgcgt taccgccttg
     8041 gacatcgatg caggccgtct gaagcgtgtg gaggacaata tcgcccgttt gggctttcag
     8101 acggcatcgg cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga tggaaagcct
     8161 ttcgacgcaa tccttgccga cgtgccgtgc accgcttccg gcgtggcgcg gcgcaatccc
     8221 gacgtgaaat ggctgcgccg accgaccgac gcgctcaaaa ccgcccgccg gcaggaagcc
     8281 ctgctggacg cactttggca ggcgttgaaa cagggcggca gaatgctgct tgccacctgt
     8341 tccgtgttcg tcgaagaaaa cgacgggcaa ctgcaaaaat tcctcaaccg ccatgccgat
     8401 gccgaaccga tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg cttttattac
     8461 gcgcttattc aaaagcatta aacaatggct tgtgctgttg ccgatgctct ccgttttgcc
     8521 ggacgcggcg gcggagggca ttgccgcgac ccgcgccgaa gcgaggataa ccgacggcgg
     8581 gcggctttcc atcagcagcc gcttccaaac cgagctgccc gaccagctcc aacaggcgtt
     8641 gcgccggggc gtaccgctca actttacctt aagctggcag ctttccgccc cgacaatcgc
     8701 ttcttatcgg tttaaattgg ggcaactgat tggcgatgac gacaatattg actacaaact
     8761 gagtttccat ccgctgacca accgctaccg cgttaccgtc ggcgcatttt ccaccgatta
     8821 cgacactttg gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga aagtcctgaa
     8881 caaaggcgcg ttgtccggtg cggaagcagg ggaaaccaag gcggaaatcc gcctgacgct
     8941 gtccacttca aaactgccca agcctttcca aatcaacgca ttgacttctc aaaactggca
     9001 tttggattcg ggttggaaac ctctaaacat catcgggaac aaataatgcg ccgcttccta
     9061 ccgatcgcag ccatatgcgc cgtcgtcctg ctgtacggat tgacggcggc aaccggcagc
     9121 accagttcgc tggcggatta tttctggtgg atagtctcgt tcagcgcaat gctgctgctg
     9181 gtgttgtccg ccgttttggc acgttatgtc atattgctgt tgaaagacag gcgcaacggc
     9241 gtgttcggtt cgcagattgc caaacgcctt tccgggatgt tcacgctggt cgccgtactg
     9301 cccggcttgt tcctgttcgg catttccgcg cagtttatca acggcacgat taattcgtgg
     9361 ttcggcaacg acacccacga agccctcgaa cgcagcctta atttgagcaa gtccgcactg
     9421 gatttggcgg cagacaatgc cgtcagcaac gccgttcccg tacagataga cctcatcggc
     9481 accgcctccc tgtcgggcaa tatgggcagt gtgctggaac actacgccgg cagcggtttt
     9541 gcccagcttg ccctgtacaa tgccgcaagc gggaaaatcg aaaaaagcat caatccgcac
     9601 caattcgacc agccgcttcc cgacaaagaa cattgggaac agattcagca gaccggttcg
     9661 gttcggagtt tggaaagcat aggcggcgta ttgtacgcgc agggatggtt gtcggcaggt
     9721 acgcacaacg ggcgcgatta cgcgctgttc ttccgccagc cgattcccga aaatgtggca
     9781 caggatgccg ttctgattga aaaggcgcgg gcgaaatatg ccgaattgag ttacagcaaa
     9841 aaaggtttgc agaccttttt tctggtaacc ctgctgattg cctcgctgct gtcgattttt
     9901 cttgcgctgg taatggcact gtattttgcc cgccgtttcg tcgaacccat tctgtcgctt
     9961 gccgagggcg caaaggcggt ggcgcagggt gatttcagcc agacgcgccc cgtattgcgc
    10021 aacgacgagt tcggacgttt gaccaagctg ttcaaccata tgaccgagca gctttccatc
    10081 gccaaagaag cagacgaacg caaccgccgg cgcgaggaag ccgcccgcca ctacctcgag
    10141 tgcgtgttgg atgggttgac taccggtgtg gtggtgtttg acgaaaaagg ccgtttgaaa
    10201 accttcaaca aggcggcgga acagattttg gggatgccgc tcgcccccct gtggggcagc
    10261 agccggcacg gttggcacgg cgtttcggca cagcagtccc tgcttgccga agtgtttgcc
    10321 gccatcggtg cggcggcagg tacggacaaa ccggtccagg tggaatatgc cgcgccggac
    10381 gatgccaaaa tcctgctggg caaggcgacg gtattgcccg aagacaacgg caacggcgtg
    10441 gtgatggtga ttgacgacat caccgtgctg atacgcgcgc aaaaagaagc cgcgtggggt
    10501 gaagtggcga agcggctggc acacgaaatc cgcaatccgc tcacgcccat ccagctttcc
    10561 gccgaacggc tggcgtggaa attgggcggg aagctggacg atcaggacgc gcaaatcctg
    10621 acgcgttcga ccgacaccat catcaaacag gtggcggcgt taaaagaaat ggtcgaggca
    10681 ttccgcaatt acgcgcgcgc cccttcgctc aaactggaaa atcaggattt gaacgcctta
    10741 atcggcgatg ttttggccct gtacgaagcc ggcccgtgcc ggtttgaggc ggaacttgcc
    10801 ggcgaaccgc tgatgatggc ggcggatacg accgccatgc ggcaggtgct gcacaatatt
    10861 ttcaaaaatg ccgccgaagc ggcggaagaa gccgatatgc ccgaagtcag ggtaaaatcg
    10921 gaaacggggc aggacggacg gattgtcctg acggtttgcg acaacggcaa gggattcggc
    10981 aaggaaatgc tgcacaatgc tttcgagccg tatgtgacgg ataagccggc gggaacggga
    11041 ctgggtctgc ctgtagtgaa aaaaatcatt ggagaacacg gcggccgcat cagcctgagc
    11101 aatcaggatg cgggtggggc gtgtgtcaga atcatcttgc caaaaacggt agaaacttat
    11161 gcgtagcagc gatattttaa ttgtagacga cgaagtcggc atccgcgacc tgctgtcgga
    11221 aatcctgcag gacgaaggct attcggtcgc gttggcggaa aatgccgaag aggcgcgcaa
    11281 gctgcgccat caggcgcgcc cggcaatggt gctgctggat atttggatgc ccgattgcga
    11341 cggtatcacc cttttgaagg agtgggcgaa aaacgggcag ctcaatatgc cggtggtggt
    11401 gatgagcggg catgccagca tcgataccgc cgtagaagcg accaaaatcg gcgcgatcga
    11461 ttttttggaa aaaccgattt ccctgcaaaa gctgctgtct gcggtcgaaa acgcgttgaa
    11521 acacggtgcg gcgcaaatcg aaacgggacc ggtgttcgac aaactgggca acagtgccgc
    11581 gattcaagag atgaaccgcg aagtcgaagc ggcggcaaag cgcacgtccc ctgtattttt
    11641 gacgggcgag gcgggttcgc cgtttgaaac cgtggcgcgc tatttccata aaaacggtac
    11701 gccgtgggtc agtccggcaa gggtcgaata tctgatcgat atgccgatgg aactgttgca
    11761 gaaggcggag ggcggcgttt tgtatgtcgg cgacatcgcc caatacagcc gcaatatcca
    11821 aaccggcatt acctttatca tcggcaaggc agaacgctgc cgtgtcaggg tgattgcttc
    11881 gtgcagctac gcggcaggtt cagacggcat ttcatgtgaa gaaaaattgg ccgggctttt
    11941 ttcggaatcg gtcgtccgta ttccgcccct gagtatgcag catgaagaca ttcccttcct
    12001 gatacagggg attgcctgca atgtggcgga aagccaaaaa atcacgccta ccgcattcag
    12061 tgacgatgcg cttgccgtat tgtcccgtta cgactggccg gggaatttcg agcagttgca
    12121 aaacgtcgtg acaacgctgc tgctggaggc ggacgggcag gaaatcggta tcggcgcggc
    12181 ttcttcggca ctggggcgcg gtgtgcctgc cgacggaacg gggcatatgg cgtgcgggtt
    12241 cgactttaac ctgccgctgc gcgaattaag ggaggaggtg gagcggcgtt atttcgagta
    12301 ccacatcgcc caagaaggtc agaatatgag caaagtggcg cagaaggtcg gtttggaacg
    12361 cacgcacctt taccgcaaac tcaaacagct cggcatcggc gtttcgcgcc gggcggggga
    12421 aaaaaccgaa gaataggccc ggacggccgg tttcccggct gcgggctttt gttttcagac
    12481 ggcatttgga gaaaatgccg tctggaatcg taaggggatg gattttatga cagaggacga
    12541 acgtttcgcg tggctgcaat tggcgtttac gccctatatc ggcgcggaaa gtttcctgct
    12601 gctgatgcgc agtttcggca gcgcgcaaaa tgccttgtcc gcaccggcgg aacaggttgc
    12661 cccggcggtg cggcataagc acgcgcttga ggcttggcgc aatgcggaaa aacgcgcttt
    12721 ggcgcggcag gcggcagaag cggcgttgga atgggaaatg cgggacggat gccgcctgat
    12781 gctgcttcag gatgaagatt tccccgaaat gctgacgcag gggctgaccg cgccgccggt
    12841 tttgtttttg cgcggcaacg tgcggctgct gcacaagcct tccgccgcca tcgtcggcag
    12901 ccgccatgcc acgccgcagg cgatgcggat tgccaaagat ttcggcaggg cgttgggtgg
    12961 gaaaggcatt cctaccgtgt cgggtatggc ttcgggcatc gacaccgccg cccatcaggg
    13021 cgcactggag gcagaaggcg gcaccatcgc cgtgtggggg acgggcatag accgcattta
    13081 tccgccggcc aacaaaaacc ttgcctatga aatcgccgaa aaagggctga ttgtcagcga
    13141 gttccccatc ggcacgcggc cgtatgccgg caactttccg cgccgcaacc gcctgattgc
    13201 cgcgctgtcg caggttacgc tggtggtcga ggccgccttg gaatccggtt cgctgattac
    13261 cgccggcctg gcggcagaaa tggggcgcga agtgatggcg gtacccggtt cgatagacaa
    13321 cccccacagc aaaggctgcc acaaactgat taaagacgga gcgaaactga cggaatgttt
    13381 ggacgacatc ctgaacgaat gcccggggct attgcaaaat acgggtgctt cgtcatattc
    13441 tataaataag gatacgcccg acacgggccg ccgcgccgtt cagacggcat acgccccgcc
    13501 gcctgccgca aaaatgccgt ctgaaggggc ggcaggcggc acagccgtcg gcggcatact
    13561 ggataaaatg ggtttcgacc ccatccatcc cgacgtgctt gccggacagt tggctatgcc
    13621 tgccgcagat ttgtatgccg cactgttgga attggaattg gacggcagcg ttgccgcaat
    13681 gcccggcggc agataccagc gtatccgaac ttgaacgcac tttatattaa ggaacacgaa
    13741 tgaccgaagt catcgcctac ctcatcgaac atttccaaga tttcgatacc tgcccgccgc
    13801 ccgaagactt gggtatgctg cttgaagaag cgggtttcga tacgatggaa atcggcaaca
    13861 ccctgatgat gatggaagta ttgctcaaca gctccgaatt ttccgccgaa cccgccggca
    13921 gcggcgcatt gcgcgtgtac agcaaagaag aaaccgacaa cctgccgcag gaagtgatgg
    13981 ggctgatgca gtatctgatt gaagaaaaag ccgtcagctg cgaacagcgg gaaatcatca
    14041 tccacgcgct catgcacatc cccggcgacg aaattaccgt agataccgcc aaagtgctga
    14101 ccctgctgct tttatgggca aacaagagcg agctgcccgt attggtcggc gacgagctga
    14161 tgagcgcgct tttactcgac aacaaaccca cgatgaactg aagcggcttc agacggcgcc
    14221 ggcccgcccg atgccgtctg aaacaccggc gtcaaaacca ccatccagag aacgacaaat
    14281 ggcgaaaaac ctcttaatcg tcgaatcccc gtccaaagcc aaaaccctga aaaaatattt
    14341 gggcggcgat tttgaaatcc tcgcgtccta cggacacgtc cgcgacctcg tccccaaaag
    14401 cggcgcggtc gatcccggca acggctttgc gatgaaatac caactcatca gccgcaacgc
    14461 caaacacgtc gatgccatcg tcgccggagc caaagaagcc gaaaacatct acctcgcaac
    14521 cgacccggac agggaaggcg aagccatttc ctggcatctt ttggaaatcc tcaaatccaa
    14581 acgcggcctg aaaaacatca agccgcagcg cgtcgtgttc cacgaaatca ccaaaaacgc
    14641 cgtgctcgat gcggttgccc atcctcgcga aatcgaaatg gatttggtcg atgcgcaaca
    14701 agcccgtcgc gctctggact atctggtcgg tttcaacctc tcgccgttgc tgtggaaaaa
    14761 aatccgacgc ggtttgagcg cgggccgcgt gcaaagccct gctttgcgcc tgatttgcga
    14821 acgcgaaaac gaaatccgcg cgtttgaagc gcaggaatat tggacggtac atctagacag
    14881 ccacaaaggc cgcagcaagt tcaccgccaa actcgcccaa tacaacggcg cgaaactcga
    14941 acaattcgac ctgccggacg aagccgctca agccgacgtg ttgaaagaac tcgaaggcaa
    15001 agaggctgtc gttactgcca ttgaaaagaa aaagcgcagc cgcaaccccg ccgcgccgtt
    15061 caccacatcc accatgcagc aggatgccgt gcgcaaactc ggcttcacca ccgaccgcac
    15121 catgcgtacc gcccagcagc tttacgaagg tatagacgta gggcagggcg ccatcggtct
    15181 gattacctat atgcgtaccg acagcgtgaa tttggctgac gaagcgttaa ccgaaatccg
    15241 ccattacatt gaaaacaaaa tcggcaaaga atatctgccg agtgccgcca aacaatacaa
    15301 aaccaaatcc aaaaacgccc aagaagccca tgaagcgatc cgcccgactt ccgtgtaccg
    15361 cacgcccgaa agcgtcaaac ccttcctgag tgcagaccag ttcaaactct atcaaatgat
    15421 ttggcagcgt accgttgcct gtcagatgac gcccgccaaa ttcgaccaaa ccaccgtcga
    15481 tattaccgtc ggcaaaggcg tgttccgcgt aaccggacaa gtgcaaacct tcgcaggctt
    15541 cctcagcgtt tacgaagaaa gcagcgacga cgaagagagc gaagacagca aaaaactgcc
    15601 cgaaatgagc gaaggcgaca aattgccggt cgacaaactc tacggcgaac aacacttcac
    15661 caccccgccg ccacgctaca acgaagccac actggttaaa gcccttgaag aatacggcat
    15721 cggccgcccc tcgacctacg ccagcatcat ctccacgctc aaagaccgcg aatacgttac
    15781 ccttgagcaa aaacgcttca tgcccaccga cacaggcgac atcgtcaata aattcctgac
    15841 cgaacacttc gcccaatacg tcgattacca cttcaccgcc aaactcgaag accagcttga
    15901 cgaaatcgcc gacggcaaac gccgctggat tcccgtgatg gacaaattct ggaaaccgtt
    15961 catcaaacaa gtggaagaaa aagagggcat cgaacgcgcc aaatttacca cgcaggaact
    16021 taacgaaacc tgcccgaaat gcggcgaaca caaactgcaa atcaagttcg gcaaaatggg
    16081 ccgcttcgtc gcctgcgccg gctatcccga atgcagctac acgcgcaacg tcaacgaaac
    16141 cgccgaagaa gccgccgagc gcatcgccaa agccgaggct gaacaagccg aactcgacgg
    16201 acgcgaatgc cccaaatgcg gcggtcggtt agcgtataaa tacagccgta ccggcagcaa
    16261 attcatcggt tgcgccaact accccaaatg caaacacgtc gagccgttgg aaaaacccaa
    16321 agataccggc gtccaatgcc cgcaatgcaa aaaaggcaac ctcgtcgagc gcaaatcccg
    16381 ctacggcaaa ctgttttaca gttgcagcac ctatcccgac tgcaactacg ccacttggaa
    16441 cccgcccgtt gccgaagaat gcccgaactg ccattggccg gtcttgacca tcaaaaccac
    16501 taaacgccgg ggtgtggaaa aagtctgtcc gcaaaaagaa tgcggctgga aagaacagat
    16561 tgaactgcct gcaccgcaag agtgaagcgg ttgggattaa gatgaaagaa aatgccgtct
    16621 gaaaggggtt tcagacggca tttgtaaatt agaaggggct gtcccagatg gctggggaaa
    16681 tttaaattaa gtcagaatta tccctatgag aaaagccgtc caagccggta caaacaaaag
    16741 aaacccatcg gactgtttgc cgcaggcgta actgcaagaa cggcagcagg gttggcagca
    16801 gttaatcaaa acacggcagc ctgttatttt ctatggcgaa atagtgtttg gcacatcatc
    16861 gggaaaactt cctttacacg catttggacg aaatctgcga aattatggag gcatacgaca
    16921 tatcgtttac gattacttat ccgtcaaaac agtccgcatt tggaaatgta tgacggcgaa
    16981 gcaggggcgg gtgaaagtta tttccgtaaa ggcagacgcg gccgcagtgc cgccggcaaa
    17041 gccgccgtat tcgggctttt gaagcgcaac ggcaaggttt gtacggttac tgtcggacac
    17101 tcgaaccgct attttattgc ctattatctg cggacgggtg aaacccggca gcattgttta
    17161 tacggattgt tcgtagttac gatgtatcag attgtaagcg gatttggccg tttccgtatc
    17221 aatcgcggca cacattttgc cgaacggcaa aaccatatca acgcaattgg gaacttttgg
    17281 aatcgggcaa acgtcatttg cgcaagtttg acggcattcc caaagagcat ttcgggctgt
    17341 atttgaagga gtgcggacgg cgttttgaca acagtggggt aaaagttcga atttccattt
    17401 taaaacaatt ggtaaagcag gatt
//
LOCUS       DAWTHD010000039        17361 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWTHD010000039 DAWTHD010000000
VERSION     DAWTHD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.039"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(174..998)
                     /locus_tag="WM704_001011"
     CDS             complement(174..998)
                     /locus_tag="WM704_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFA9340453.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPVPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(1111..2889)
                     /locus_tag="WM704_001012"
     CDS             complement(1111..2889)
                     /locus_tag="WM704_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFA9340454.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            complement(2886..3392)
                     /gene="rfaE2"
                     /locus_tag="WM704_001013"
     CDS             complement(2886..3392)
                     /gene="rfaE2"
                     /locus_tag="WM704_001013"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFA9340455.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNSLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            complement(3604..3679)
                     /locus_tag="WM704_001014"
     tRNA            complement(3604..3679)
                     /locus_tag="WM704_001014"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3644..3646),aa:His,seq:gtg)
     gene            complement(3707..3783)
                     /locus_tag="WM704_001015"
     tRNA            complement(3707..3783)
                     /locus_tag="WM704_001015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3747..3749),aa:Arg,seq:tct)
     gene            complement(3808..3885)
                     /locus_tag="WM704_001016"
     tRNA            complement(3808..3885)
                     /locus_tag="WM704_001016"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3848..3850),aa:Pro,seq:tgg)
     gene            4108..4962
                     /gene="folD"
                     /locus_tag="WM704_001017"
     CDS             4108..4962
                     /gene="folD"
                     /locus_tag="WM704_001017"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFA9340456.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            complement(5024..5605)
                     /locus_tag="WM704_001018"
     CDS             complement(5024..5605)
                     /locus_tag="WM704_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFA9340457.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            5780..6895
                     /gene="asd"
                     /locus_tag="WM704_001019"
     CDS             5780..6895
                     /gene="asd"
                     /locus_tag="WM704_001019"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFA9340458.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            complement(6988..7518)
                     /locus_tag="WM704_001020"
     CDS             complement(6988..7518)
                     /locus_tag="WM704_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9340459.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(7528..7872)
                     /locus_tag="WM704_001021"
     CDS             complement(7528..7872)
                     /locus_tag="WM704_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340460.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            complement(7859..8638)
                     /gene="xth"
                     /locus_tag="WM704_001022"
     CDS             complement(7859..8638)
                     /gene="xth"
                     /locus_tag="WM704_001022"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFA9340461.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            complement(8809..10230)
                     /locus_tag="WM704_001023"
     CDS             complement(8809..10230)
                     /locus_tag="WM704_001023"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFA9340462.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            complement(10246..11298)
                     /locus_tag="WM704_001024"
     CDS             complement(10246..11298)
                     /locus_tag="WM704_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFA9340463.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            complement(11295..12242)
                     /locus_tag="WM704_001025"
     CDS             complement(11295..12242)
                     /locus_tag="WM704_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9340464.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            complement(12296..13450)
                     /gene="obgE"
                     /locus_tag="WM704_001026"
     CDS             complement(12296..13450)
                     /gene="obgE"
                     /locus_tag="WM704_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFA9340465.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13729..14259
                     /locus_tag="WM704_001027"
     CDS             13729..14259
                     /locus_tag="WM704_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFA9340466.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            complement(14294..15169)
                     /gene="rsmI"
                     /locus_tag="WM704_001028"
     CDS             complement(14294..15169)
                     /gene="rsmI"
                     /locus_tag="WM704_001028"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFA9340467.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            15216..15563
                     /locus_tag="WM704_001029"
     CDS             15216..15563
                     /locus_tag="WM704_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFA9340468.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            15568..16161
                     /locus_tag="WM704_001030"
     CDS             15568..16161
                     /locus_tag="WM704_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFA9340469.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            16225..16833
                     /locus_tag="WM704_001031"
     CDS             16225..16833
                     /locus_tag="WM704_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFA9340470.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            16841..>17361
                     /locus_tag="WM704_001032"
     CDS             16841..>17361
                     /locus_tag="WM704_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340471.1"
                     /translation="MPFEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPPPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETP"
BASE COUNT         3968 a         5025 c         4440 g         3928 t
ORIGIN      
        1 caaaaccact cggaagccgt ccgttccgaa ccattaaaca ctgtattccc tcacaattca
       61 ctttcacact tggagtcggc atatacgaga catacattcc ctttttaatc agatactcaa
      121 aaccgaaacg ccaaacccac cctagcggtg ggtttggcgt ttttggtctg cacttatttg
      181 caagacttga ggttcagttt gccgtatagg gacgtgattt tacggatttc gtccgcatcg
      241 gcggcattca cgcccgtaaa caaaaccgtc atacgcgaca cgctcaaaga atcgtcctgc
      301 ctgtcggctt cggcaaagtg ttcgacaata tgcgccccgc cgaatccggc atcggcaagt
      361 cggttttgca gggcttgggc gttttcccgg ctgttgccga cacccaaact caatgcgccg
      421 tcaaacggta cggggttgaa acctttggca gacagctccg ccgcctgatt ttcggcatcg
      481 gcggaaacgg gcaggacgac gcggtaggtt ttgtcggcag gtttggcttt ggcggtgcgt
      541 ttttcgacgc tcctgctggc aacgtgcgac catttgccca aaagcccttt gatgcggtgg
      601 tagtcgtctt cgtccatcgt gaggcttgcc tgcgcggcgc ataaggcatc cgccgcgccg
      661 ttttcacgtt ccgcctgcgc cttttcggcg gcgagttttt cgcggcgggc tttttcttca
      721 cgctgttttt tctccctcag ttttttctgt tccgcttctt ttttcaagcg caactgctcc
      781 gcctgttctt cgctcaggat gtcgcccggt ttgagcagtg ccgccgtatc ctccacggat
      841 gccgccgcat tgccggcggc gggcgttgcg gggggcgtgt tttcgacggc ccggttttcc
      901 ggcacgccgc ccgccgcttt aacggccaat ttatagccca ccgtgccgcc gaatacggca
      961 atattgagta ccgccaaaag ggtgaagagc catttcatta gaatttcctc aatgtttgaa
     1021 accccgcccg cccgggcggc agtctgtcgg gcggcagaat ccggacagat tcggacggat
     1081 gccgccccgt gttgaaaaaa ggcatatttt ttaagcgtgt tccgattccc cgccttcggc
     1141 ggcaatcagg ttcagcagcc cgtggatgac gaggttgtcc gccacgcgca cggtattttc
     1201 cgccaaaaat gcaggcggca gggcttcggc gactttcgcc gcgccgccgc cggtaatgat
     1261 gacatcgaca ggcttgcccg cgccgttttt ttctttcaaa cggccgtgca tcatcattat
     1321 cgagccgcaa accgcgtcca tcatgccgct tgcgacggcg ttgcccgttg tggtcgggaa
     1381 agggtaacgt ttgccggcgg ggcggttgag gttggcggtt cggacggcga gcgattcttt
     1441 catcaggtgg aagccgggca tgatggttcc gccgagataa tgtccgtcat cggtgagcgc
     1501 gtcaaccgtt accgccgtgc cgcaactgac gacgacgcag gcgttgcggc tgaagcggcg
     1561 gctgcccaag gcgttgaacc aacggtcgga accgtgttct tcggggtggc ggtagtggtt
     1621 gcgtatgccc aaagcctgtg cggaagacgg cagccactcg atttttcggg cgagctgttc
     1681 cttcacttgt gcctttttgg attctccgca cacggcgcaa ccgacgatgc ggacatttcc
     1741 atccgccttt tccgcccact ccgcgcccaa aggcgacaaa tcgcggtacg gcgcgctgcc
     1801 gacggttgcg aacgtgccgt tttccaccca cgcccacttg agccggctgt tcccgccgtc
     1861 caacagcaaa aaacgttccg aatcccgccg cttcggcacg gaaaccggtc tgtcgtcggg
     1921 ccgcaggctg atttcgccgc tgacgaccgt ctgttcgccc tctgccgttt ccaagtgcag
     1981 aacgcctcgt ccgtccacgc ctttaaccgt gccttcgcac acggtttcgc cgtcgcgcaa
     2041 cagcaatacc gccttgccgt ggtcgcggtt ggccgtttca tactcattta aaaatggcgc
     2101 gaacccttct tccgcatatt gttccaacac cgcgcccagt tccgcaagca atgtttccag
     2161 caatacggcg gcatcggcat tgccccgccg cgatgccgtc tgaaacagcg actgcacgga
     2221 agcggcgttt tccacttcct tgggcagcac gaaattgatg ccgataccga ccacggcaac
     2281 cgttttaccg cccgccctga ctgtttcaat cagaatgccg cccaatttgt cgcgtccgac
     2341 gaccaaatcg tttggccact tgatttgcgt ttccaaaccc aaacacccca aagcgcgccg
     2401 gcacgcaagt gccgcaacag gcgacagcga acccaactca tactgcggcc ggtcaaacgc
     2461 ccagccgaaa ctgaacatca ggcactcgcc caaacggtgc gaccacttcc gcccctgccg
     2521 ccccctgccc ttactttgca ggtgggtcac gcatatggtt ttgtgcgcct tgtccggcgc
     2581 aatccgcgcc aattccagta tctcgtcgtt gctggacgcg cactcgtgct tcaatgccgt
     2641 ctgaaaaccc gacctttccc ccagatcgcg caaaccttcg gcatcgaaaa ccgccaaggg
     2701 gcgcaccagc cgccaatagc cgtcgtgttg gcgcaacagc ccgcgtatat gcgccggcat
     2761 ctgctgccaa aaaccgttga gctgctgcgg cttcatgtcc gcctcacgcg ccaattgcga
     2821 tacgtgttgc ggcaaaccgt cggcaagctc cgccaacacc cgccagtgcg aaggcttcaa
     2881 aaccgtcatt ttccgccctc tgccgcacgg atttttgcca aagtcttcgt tgtcgaagtc
     2941 tgatgcagaa acggaattga aaacacctga ccgccgcgtg ccagcgtttc cgccgcaccg
     3001 acaatcttat ccacgaccca atcgccgccc ttgaccaaca cctccggctt gaccgcctca
     3061 atcaacgccg ccggcgtatc ctcgtcaaac caagtaacca aatccacact ttccaacgcg
     3121 gcggcaacgg cggcacggtt ctccaaagaa ttaaccgggc ggtcgccgcc cttgcccaaa
     3181 cgccgcaccg aagcatcggt attcaacgcc agcaccaacg ccgcccctgc cgaacgcgcc
     3241 tgcgccagat aagtaacgtg ccccctgtgg aggatgtcga aacagccgtt ggtaaacacc
     3301 agcgggcgcg gcaacaacgc caaacgcgcc gccaacgcct cgggcggaca gattttcgat
     3361 tcaaaatcag ggacagacca agcgtcaacc atcaaagcct ccgacaaaaa ccataaaaga
     3421 cagaaaaacc cacatgatac agaagcatat gcgaaaggca aagccggcgg cgcaaatgcc
     3481 gtatgaagcg gtttccgtat gaccggcccg gctttcagac ggcataccgc cgcaccggta
     3541 cgacggcgcg gacagtacgc gcaaacagga aaagacccgt accgaaaagt acgggccttt
     3601 atctggggtg gctgatgggg ctcgaaccca cgacaaccgg aatcacaatc cggggctcta
     3661 ccaactgagc tacagccacc ataaaaacgg ttttcaatca aattcttggc acgcccgaca
     3721 ggaatcgaac ctgtaacccc cgacttagaa ggtcggtgct ctatccggtt gagctacggg
     3781 cgctcatgcc gattcgtgct gattgattgg tcggggcggt gggattcgaa ctcacgaccc
     3841 tctgctccca aagcagatgc gctaaccggg ctgcgctacg ccccgacttg aagaagcgga
     3901 atatacagtt cagggaaaga tgcgtcaaca tttattttca aaacaccaag atgaaaaata
     3961 tagttttttg atttgaaaaa atatttaatc cgtccaaaca gccgtatttt atttcggggc
     4021 aaatttattt tcggcattct gctgtaaaaa caaacggaaa atgcgataat tttcagcatt
     4081 ttctacctgt ttaacaaaag gacggatatg tcggcacaac tgatcaacgg taaagaagtt
     4141 tcgcaaaaac acctgcaggc gattgccgaa gcggtggcgc aacgccaaca ggacaatctg
     4201 cacacgcctt gcctggccgt ggttttggtc ggaggcgacc ctgccggcgc agtttatgtc
     4261 cgcaacaaga aaaccgcctg ccaaaaatgc ggcatcaagt cgctgtcgta cgaactgccc
     4321 gaatcaacat cgcaggaaga actgctggca ttggtcgacc gtctgaatgc cgattccgaa
     4381 gtggacggta ttctggttca gcttccactg ccgaagcacc tcgacagcca agcgatcttg
     4441 gaacgtattt cgccggataa ggacgtggac ggcttccatc cttacaatgt cggcaggctg
     4501 gcggtcaaaa tgccgctgat gcgcccgtgt acgcccaagg gcgtgatgac gcttttggag
     4561 gcttacggca ttgatccgaa ggggaaaaaa gcggtcgtgg tcggcgcgtc gaatattgtc
     4621 ggccgcccgc aggcgttgga attgctgctg gcgcgcgcaa cggtaacggt ctgccacagt
     4681 gcgaccgaaa atcttgccga tgaagttgcc gccgccgata ttttggtggt cggcgtgggc
     4741 attccgaact ttgtcaaagg cgggtggatc aaacccggcg cggtcgttat cgatgtgggc
     4801 atcaaccgtt tggacgacgg cagcctgtgc ggcgacgtgg aatttgaaac ggcaaaagaa
     4861 cgggcggcga tgattacgcc cgttcccggc ggcgtgggtc cgatgacgat tgccacatta
     4921 atggaaaaca ctttgcacgc agcttcgctg cacgatgctt gagcggttct gaagataaaa
     4981 atgccgtctg aaagcctttc agacggcatt ttgccgcgtc cgtttatttg ggcagcttga
     5041 cgacaaccgt atccgccaat atgtcgtaaa gcgtgcggcg gtcgcgtttg accataaaga
     5101 gcaggacaaa gttggcaagg aatgccagca ggttgatggc gttttccccg ttttcaccta
     5161 ctgcaagacc gataacggcg gcaataatgg caaccaaaac cgaccacgcg atttcgcgta
     5221 ccaaaaccgt gccgacaaaa cccggattgc ggccgtcggt tttcaacacg cggattctca
     5281 tgattttctt acccaatgac tgcccgtccc tgctcatata gtagatttgg atgacggtgt
     5341 acgccaaaat gcccgccagt ccgacccaaa aggaagtcat gcccaaaagc agcccgaata
     5401 tttcctcacg accgccaatc ctgccttcat ttttgacggc gaaggcaatc agtccgacaa
     5461 acggcaccaa caaaatcaaa aaggtaaaca attggttcag cagcgcggca agtatccggt
     5521 cgcctgcgcc ggcaattccg acttcaattt cctgcccgtt gcggttgtcg gataccgcgt
     5581 cggtgtagtc gtttttttct tccatatccg ttcctgataa ttgttcttaa acagatggaa
     5641 ttctaccgcc ctactgctgc aaacgccaat cccaaaaaga aaattcgata aagaacttta
     5701 cattttccca atacggcgtt aaaacgcttt ctttacgcca tacataattt tattaacgat
     5761 tttcctcaag gagcaataca tgaaagtagg tttcgtcggc tggcgcggta tggtcggttc
     5821 ggttttgatg cagcgtatga aagaagaaaa cgacttcgcc cacattcccg aagcgttttt
     5881 ctttacctct tccaacgtcg gcggcgcggc acctgatttc ggtcaggcgg ctaaaacatt
     5941 attggacgcg aacgacgttg ccgagctggc gaaaatggac attatcgtca cctgccaagg
     6001 cggcgattac accaaatccg tcttccaagc cctgcgcgac agcggctgga acggctactg
     6061 gattgacgcg gcgtcctcac tgcgcatgaa agacgacgcg attatcgccc tcgaccccgt
     6121 caaccgcaac gtcatcgaca acggtctcaa aaacggcgtg aaaaactaca tcggcggcaa
     6181 ctgtaccgtc tccctgatgc tgatggcttt gggcggcctg ttccaaaacg atttggtcga
     6241 atgggcgacc agcatgacct accaagccgc ttccggcgcg ggcgcgaaaa atatgcgcga
     6301 actcatcagc ggcatgggcg cgattcacgc ccaagtggcg gacgagcttg ccgatccttc
     6361 cagcgcgatt ctcgatatcg accgcaaagt gtccgatttc ctccgcagcg aagactatcc
     6421 gaaagccaac ttcggcgtac cgctcgccgg cagcctgatt ccgtggattg acgtggattt
     6481 gggcaacggc cagtccaaag aagaatggaa gggcggcgtg gaaaccaaca aaatcctcgg
     6541 acgcagcggc aatccgaccg tcatcgacgg cctgtgcgtc cgcatcggcg cgatgcgctg
     6601 ccacagccaa gccatcactc tgaagttgaa aaaagacctg cctgtttccg aaatcgaagc
     6661 gattttggca ggcgcgaacg actgggtgaa agtcgtcccc aacgaaaaag aagccagcat
     6721 ccgcgagctg acgcccgcca aagttaccgg cacgctgtcc gtccctgtcg gacgcatccg
     6781 caaactgggc atgggcggcg aatacatcag cgcgttcacc gtcggcgacc aacttttgtg
     6841 gggtgcagcc gaaccgatgc gccgcgtgtt gcgtatcgtg ttgggcagcc tgtaagccct
     6901 gtttgaatgg aaatgccgtc tgaagcctgt ttcagacggt attttccttg caaccccggt
     6961 taaccgaaat tccgataaag cgaccgttca cgccagcaac atttcctgca tcagcttcat
     7021 accccactgc cagccgccga gcataccgtt caaactgccc gaatgcgggg aaaccaacag
     7081 gcgggcgttc cacaaatccg cctgtttttg cgcccaaccg tgcggcacgc cgccgtgttc
     7141 ggacaccacc aatgcggcac ggcaaggaca gcggacgcgt tggaaagtgt gttccgcatc
     7201 gtcgggaaaa atatcgggac gcggagatac gagaatgata ccggcgagtt tcttctgcgt
     7261 caggatgtcg gcgcggtaca gccacgccaa gaatgcggcc gcgcccgcgc cgtgtgcgac
     7321 aacggcgata tgcctgccct gtatccgttc gaatgccgtc tgaagcgcgc tttgccacgc
     7381 ttccgtgctg ccgtttgcgg acgcttcgga catctgcaca acgggataac tgaccgccca
     7441 acggtctatc cacatttccg cttcatccgc atcgcgtatc agccacagcg tcaaatcctc
     7501 aagctcgaaa ctttccattg tccgcgacta tttgagcagg tcccggaggg taaaggcgat
     7561 gagcagcgaa gcgggtacgc tcaatatggc gcagacggtc aggcagacaa aaatattcac
     7621 cacccgccgc cagcctttcc aacccaaaca tttggccgca atcagcaggc agggcagaca
     7681 aatcagcagc cacaccaacg cccatatcgg gtttgccttg gtcggcgcaa gccagccttg
     7741 catccgcgac aacataaaca ccgcccacac caatatgggc aggataaacg cagcgacaac
     7801 ccatgccgcg cctattcctg tttttccgtc cacattccaa tcatatttac ccaaaacctt
     7861 attcggcagc atagtcatac tccacaacta aaggggcgtg atccgagaat ttttcatctt
     7921 tataaacgtg tgcggacacg gctttggcgg caagttcggg cgtaaccatc tgataatcga
     7981 tgcgccaccc gacatctttc gcatacgcct gcccgcggtt gctccaccat gtataacccg
     8041 gcacatcggg atagagcgtg cgccacatgt ccgtccagcc cagcgtatgg atgactttgc
     8101 ctatccactc gcgctcttcg ggcaggaagc ccgaattttt ctgattgcct ttccaatttt
     8161 tcaggtcgat gttttggtgg gcgatgttcc agtcgccgca gacgacgatg tcgcgccctt
     8221 cgtttttcat cgcttcgagc atagggtaaa acgcatcgag gaaacggtat ttcacctgct
     8281 ggcgttcttc cgcgctgctg ccgctgggca aataaagcga aatgacgccc aacctgccga
     8341 aatcgcaacg cacaaaccgc ccttcgcggt cgaattcctc aatgcccatg ccgatttgca
     8401 cattgtcggg tttgcgtttg ctgtacaccg ccacaccgct gtaaccgcgc ttctcggcgc
     8461 aatgccaata gccgtgcatc ccgtgcggat ttttcatatc ggcagacaaa tcagcctcct
     8521 gcgctttgag ttcctgcacg cagacaatgt ctgcgcccga tgcggcgatg tattcgtaaa
     8581 aacctttttt gtaggcggag cggatgccgt tgacgttggc ggaaatgatt ttaagcataa
     8641 taaaaataag ttctcacaat gttcccatac acaaagcggc acccggtgcg ccgcccggta
     8701 tttcccattc ggtcctgcct gctgaaaacg gcggggcttc aagcgggaaa tgccgtctga
     8761 aaacgaaaaa agggcaaaag gcggtacttt tgccctttcc gatacagttc aaccgcgccg
     8821 ccaagtcgtg ccgccggcgc tgtcttccaa aatgattttg tgttcgttca gaaggtcgcg
     8881 gatgcggtcg gactccgccc agtttttatc cgcgcgagcc tgtttgcgtc gggcaatcaa
     8941 gtcttcgatt tcttcgttgg agaggccgtc tgaaactgcg ccgccttgga ggaactcaat
     9001 cgggttgcgt tgcagcaggc cgatgatgcc gcccaaagct ttcaggcagc cggcgaggtg
     9061 tgcgtcattg gttttgttca cttcgcctgc cagctcaaac aacacggcaa ccgcttcgac
     9121 cgtaccgaaa tcgtcgttca tggcggcgta gaaacggcgg gtgtagccgt tggcgttttc
     9181 agacaaatca aactcagccg ccggtgtgtt tttcaaggtg gtgtacagac gggtcaacgc
     9241 gcctttcgcg tcgtccaaat gcgcgtcgga gtagttcaac gggctgcggt agtgggcgcg
     9301 caggatgaag aaacgcacga cttcggggtc gtattgtttc aatacttcgc ggatagtaaa
     9361 gaagttgccc agcgatttgg acattttttc gccgtccaca cggatgaagc cgttgtgcag
     9421 ccagtatttg acgtggctgg caatactttg accgtggtgg gtttgcgcgt ggtcatgacc
     9481 gcaggtgtga ccgctggcac cgacgctttg ggcgatttca ttttcatggt gcgggaactg
     9541 caaatccgcg ccgccgccgt gaatgtcgaa cgtatcgccg aacaggtttt cactcatcgc
     9601 ggaacattcg atgtgccaac ccggacggcc gttgccccac gggctttccc acgccggttc
     9661 gcctgctttc gccgctttcc acaatacaaa atcaagcgga tcgcgtttga aaccgtccac
     9721 ttccacgcgt tcgcccgcac gcaggtcgtc caacgatttg cccgataatt gtccgtaagc
     9781 ggcaaactcg cgcacggcgt agtaaacgtc gccgtttgcg gcaggatatg ccttgccgtt
     9841 ttgaatcagg gtctcaatca tggcaatcat ttgcgggatg ttttccgtcg ccttcggctc
     9901 gatgtcggga cgcaacacgc ccaaagcatc ggcatcttcg tgcatcgcct gaatgaaacg
     9961 cgcgttcagt tcgccgatgg tttcgccgtt ttcagccgca cgggcgatga ttttgtcgtc
    10021 gatgtcggtg atgttgcgca cataagtgag cggataaccg cactcgcgca accagcgggc
    10081 aatcatgtcg aacaccacca tcacacgggc atggcctaag tggcaatagt cgtaaacggt
    10141 catgccgcag acgtacatac gcacgttttc agggtcgatg ggggcgaagg gttctttttg
    10201 acgggtaagg gtgttgtaga tggcggtcat attcatcaag taagtttaga aatgagtaac
    10261 agtaatgtca tatccgcgat tttgggcgaa atctaaaaat tcattggctt cattaacggc
    10321 aggaaattca cgcataattt cagttttaat attttccaac aacgcttctg caatcggata
    10381 ttggcctctc aatgcttttt cgtaccaact tatcaaatca tcctcagtta cgatgttttg
    10441 aattcggctt ttccagccta tttgcgtcag taatgaaaca atcaccgatt gttctgcctt
    10501 tttgcagaca atgacaattc tatctgcgct aatggaggat acaatatctt ctgccaactc
    10561 ttcgtctaaa gagagatgtt tgacctgtat ggccaatccg aagttagacc acatatccaa
    10621 ccctctatcg gcagcattgg ttacgcctac acgatggatt tttgcaggaa gttttaaatg
    10681 ctcatttttc ggcatagtga tgattttttc ggcaaaatct tgatattcct cccataaaaa
    10741 caggttttcc ttaggaaaat caattgaaac cgttatgcct aattctgaaa ccaatgcatc
    10801 aaacaacgca taaacaacga tttcatatat cttgtctata cttcttttca atcccggctc
    10861 gagccaaaat aaatttagaa attcagacag ttggaaggag tatctgtctg tattgccgac
    10921 ataagccaat gcttcgctca tttgggaaaa acgattgaaa aactgtttat aaatgtacga
    10981 ttctaccccg ccgtccgact gtctgtttaa tgttcccaat accgctagtt tttcaggcgg
    11041 tattgcattt ttttcaaaca gattatcttg gaattttgca gatgaagtag aaatccgccc
    11101 taaaaaccga cggcagattt catccctcca gcgtttagat tggttgcggt aagtatctaa
    11161 atttaaaaaa tccaactgtt ttatacaacg gtcatgatat aaaatttctg caatttgaat
    11221 gggtttataa aggtggacac gggatttttt gataatgccg tccaatgctt ctttggcttg
    11281 ttgttcttca agcgtcatcc tgcctccaat aaatccaatt gccctttttg ttcgaccttg
    11341 ccggaaagag aagcgagcat attttccgcg atggcttcta ttacagggac ggatacagaa
    11401 tttccggcga ctttcctgac ttgcgagtag gggatattga tttcaaaatc atcgggaaaa
    11461 ccttgtaatc ttaacatttc ccttcctgtt agccttctta ctccgtttac caccaaataa
    11521 ttgtaacttc ccccggccct taaggcacag gaatatggaa gtgcggaaac attcccgcca
    11581 atattttcat gccaaattga tggggtgggc ggagcttttt ttaaggctgc caaacgtttt
    11641 tgtttcaagg tatctgataa aaaatagctt ggttcgacat ctcgatcgtt ttccagcagt
    11701 tcgcccaatg gccgatattg gtttatgggt tcgggaaagt aaaaagggat gttgtctgaa
    11761 aaacccacaa tataaattct ctcgcgtttt tgcggcaatc cgaaatccaa agtatttaaa
    11821 accttaaaat aaaccgtata accaagctgt ttaagggttt ctaaaacaat acggaaagtc
    11881 ctgcccgaat cgtgcgtagt cagccgctta acgttttcca gaagaaaagc cttcggctgt
    11941 ttggttttca ggatttcggc aatattgaaa aacaatgttc cgcgcgtatc ttcaaatccc
    12001 aatcccttac ctgcgatact gaaaggttgg cacggaaatc ccgcaagcag aatatcatga
    12061 tcgggaatat ctgaaggatc gatgccgtta atgtcgccaa aaggtttttc tccaaagttt
    12121 gcttcataca cttgtcgggc atatttgtcc cattccgatg agaatacatt ggtgcacccg
    12181 tatttttcaa agcctaggcg tattccgcca atgcctgaaa ataaatctat tgttttgtac
    12241 atagcacatc tctgcagaat gagccgcctg aaaactttca gacggccttg tccttttact
    12301 ccggcttaaa cacgccagta tccgttttag gctgctgttc gataatttca acatttgccg
    12361 ctgctttctc cgcttcggct ttttcagcct cgatacgttt tttctcggcc aggtattggt
    12421 tgatttggtg taccaactcc tgcgtgcctt gatgtgtcag cgcgctgatt tggaagaggc
    12481 gcggggtttc catgtcgaat tggaaacggt catcaggttc ggggtagtcc cagccgacgg
    12541 cttcgaggaa ggcggctgtt cgcgcccggg cttcttcttc gtcgagcata tcgagtttgt
    12601 tcagcaccag ccagcgcggt ttgccgtaaa gttcttcgtc gtatttgcgt aattcgttga
    12661 tgatggcgag tgcttcttcg gcgggattga cggtttcgtc gaagggtgcc aaatcgacaa
    12721 cgtgtaacag cagaccggtg cgtgataggt gtttgaggaa gcggtggccg aggcctgcgc
    12781 cttctgccgc gccttcaatc aggccgggga tgtcggccat cacgaagctg tggttttcgt
    12841 cgatgcgtac cacgcccaag ttcggatgca gggtggtgaa ggggtagttg gcgattttgg
    12901 ggcgtgcggc ggagacggcg gtaatcaggg tggatttgcc ggcgttgggc atgcctaaca
    12961 agccgacatc ggcgaggact ttgagttcga gttgcaggga acgggtttcg ccttcttcgc
    13021 cgggcgtgga ttgttttggg gcgcggttga cggaggattt gaagtggatg ttgcccaagc
    13081 cgcctttgcc gcctttggcg aggcagacgc gctgtccgtg gtaggtgagg tcggcaacga
    13141 tttcgtcggt gtcgaggtcg cggataaggg tgccgacggg cattttgagg acaatgtcgt
    13201 ccgcacctgc gccgtagcgg tcggaaccgt ggcctttttc gccgtttttg gcttggtagc
    13261 gtttgacgaa gcggtattcg acgagggtgt tggtgttttc gtcggcttct gcccagacgc
    13321 tgccgccttt gccgccgtcg ccaccgtccg ggccgccgcg gggtacgaat ttttcgcggc
    13381 ggaaactggt tgcgccatta ccgcctttgc ctgcggcgac ttcgattttt gcttcgtcga
    13441 tgaatttcat taaatgctct tgtttgttgg ttttaaatag ggtttcaggc ggattgccat
    13501 atgttttgat gccgtctgaa cagattttct gccgacatta taaggtataa gcggtatttc
    13561 aatataccgc ggagaagcta tttgttctgc ccatcttaat gaatttttaa gcaaatcttc
    13621 agcctgcaaa caaaatatgt ccaacttctt tggtacaatc gcgccttttt gacattccga
    13681 cccgacggaa tgtccgttca aaccgttaca tatgataagg atttttttat gaacacaaac
    13741 caacctgccg tttacgaccc gttgacacgc gcgctgcact ggctgaccgt tgccggcttc
    13801 atcggcattc tgaccaccat tgtcctgtgg acaatttacg aagaggcgga atgggcgggc
    13861 agcctgttcg gcctgcacaa atctttcggt ttcctcatgc tgacggtgat tgcattgcgc
    13921 atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa gcgacagcaa ggcggcagcg
    13981 gcaggacacg gcattctgta tctgctcatg cttgccgttc ccgttatcgg catgatccgc
    14041 caatacggtg gcggacgcgg cccgttgaaa gtgttcggcg ttgaagtaat gcagggttcg
    14101 ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt tccacggcaa tttgggctgg
    14161 ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg tcgtccatcg tgttcaaggc
    14221 aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat tccgttcgca ctatggtgcc
    14281 ggtccatccg gcatcatttg tttttccacg acagtgccaa atcgtacaaa gcctttttgc
    14341 cctcacctgt aatcttggcg gcaagctccg ccgcctgctt ggtcggcagc tcggccgcaa
    14401 ggattttcat cgcattttgc gcagactcgg acaagccttc gtgtttttca tcctgcgccg
    14461 gataaagcac caacaccatc tcgccgcgcg attggttgcc gtccgccgcc aatgccgtct
    14521 gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt tttcgtgatt tcgcgcgcca
    14581 gcatcagacg gcgttcgggg aacaattccg ccatatcggc aagcgttgcc ccgattcggt
    14641 gcggcgtttc aaacatgacg acaggaaatg ccgcccgcac ccatttggca aacaatttcc
    14701 tacgttcgcc cgatttcggc ggtacaaaac cgttgaaata aaaatcggat tccgccacac
    14761 cggccacact caacgccgcc attaccgcgc ttgcgcccac gacgggaacg actttgaacc
    14821 ctgcttcgcg cacgcggcgg gcgagtttcg cgcccgggtc gcacacggcc ggcgtacccg
    14881 catcggaaac ctgcgccaca accaggccgt ctgaaaggaa accgattacc ttgtccgcca
    14941 tctgccgctc gttgtgttcg cgcacactga ccaacctgcc ctgaatgccg tacgcgctca
    15001 aaagctgcgc agtaacgcgc gtgtcttcgg cacaaatgat gtccgccttt tgcaataccg
    15061 ccaaagcgcg cagggtaatg tctgccaaat tgccgatggg cgtggcaacc acgtataatg
    15121 tccctccgac gacgctgtcg ggggctttct gcaagtgttt ctgaaacata aaaaatgccg
    15181 tctgaaaaac agacattata aaggttaaac cgattatgcg cttaaaccac aaacagggta
    15241 cggcagggga agatgccgca cttgccttcc tccaatccca aggctgcacg ctgcttgccc
    15301 gcaactggca ctgcgcctac ggcgaaatcg acctgattgt caaaaacggc ggcatgattc
    15361 tgtttgttga agtaaaatac cgcaaaaacc aacggttcgg cggcgcggca tacagcattt
    15421 ctccgtccaa attattgaaa ctgcaacgaa gtgtagagta ttatctgcaa cagaacaggc
    15481 tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga aggaaaccgc ccgcccgagt
    15541 ggataaagaa tattacaggt tgacgatatg acgacattac aagaacgcgt tgccgcacat
    15601 tttgccgaaa gcatccgtgc caaacaggaa gccgaaaaaa tactggtcga gccgaccgta
    15661 caggctgccg agctgatgct gcaatgcctg atgaacgacg gcaaaatcct ggcctgcggc
    15721 aacggcggtt cggctgccga cgcgcaacac ttcgccgccg aaatgaccgg gcgttttgag
    15781 aaagaacgca tggaactcgc cgccgtcgcc ctgaccacag acacctccgc gctgaccgcc
    15841 atcggcaacg actacggttt cgaccacgta ttcagcaaac aagtgcgcgc gctcggacgt
    15901 gcaggcgacg tcctcgtcgg catttccacc tccggcaatt ccgccaacgt catcgaagcc
    15961 gtcaaagccg cgcacgaacg cgatatgcac gtcatcgccc tgaccggccg cgacggcggc
    16021 aaaatcgccg ccatgctcaa agacaccgac gttctgctca acgtccccca tccgcgcacc
    16081 gcccgcattc aggaaaacca catcctgctg atacacgcca tgtgcgactg tatcgactcc
    16141 gtactgctgg aaggaatgta acccttttca gacggcatgg cgcaaagcaa tgccgtctga
    16201 aacgcccaag aaaggaagtc cccgatgaaa cccaaaccac acaccgtccg caccctgatt
    16261 gccgccgtcc tcagccttgc cctcggcggc tgcttcagcg cagtcgtcgg cggggccgcc
    16321 gtcggcgcga aatccgtcat cgaccgccga accaccggcg cgcaaaccga tgacaacgtt
    16381 atggcgttgc gtatcgaaac caccgcccgt tcctacctgc gccaaaacaa ccaaaccaaa
    16441 ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc acctgctgct gctcggacaa
    16501 gtcgccaccg aaggcgaaaa acagttcgtc ggtcagattg cacgttccga acaggccgcc
    16561 gaaggcgtat acaactacat taccgtcgcc tccctgccgc gcactgcggg cgacatcgcc
    16621 ggcgacactt ggaacacgtc caaagtccgc gccacgctgc tgggcatcag ccccgctaca
    16681 caggcgcgcg tcaaaatcat tacctacggc aatgtaacct acgttatggg catcctcacc
    16741 cccgaagaac aggcgcagat tacccaaaaa gtcagcacca ccgtcggcgt acaaaaagtc
    16801 attaccctct accaaaacta cgtccaacgc tgactcggca atgccgtttg aaccgccttc
    16861 agacggcatt gcccgacacc ccaaaagcac aatcaaaatg gcaaaaaaac cgaacaaacc
    16921 cttcaggctg acccccaaac tcctgatacg cgccgtattg ctcatctgta tcaccgccat
    16981 cggcgcattg gcagtaggca tcgtcagcac attcaacccg aacggcgaca aaaccctcca
    17041 aaccgaaccg caacacaccg acagcccccg cgaaaccgaa ttctggctgc caaacggcgc
    17101 cgtcggacaa gatgccgccc aacccgaaca ccaccacgcc gcctcatccg aacccgcaca
    17161 gccggacggc acagaagaaa gcggcagcgg actgccgccc cctgccgcac ccaagaaaaa
    17221 ccgggtcaaa ccgcgccctt cggatgcggc ccgggcagcc gattcgttaa ccggcaccgg
    17281 aacacaagct gaaaacacac tcaaagaaac ccccgtactg cccacaaacg ccccccatcc
    17341 cgaaccccga aaagaaacac c
//
LOCUS       DAWTHD010000040        17300 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWTHD010000040 DAWTHD010000000
VERSION     DAWTHD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17300
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.040"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            59..538
                     /locus_tag="WM704_001033"
     CDS             59..538
                     /locus_tag="WM704_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340472.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SAGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            633..1256
                     /gene="rsmG"
                     /locus_tag="WM704_001034"
     CDS             633..1256
                     /gene="rsmG"
                     /locus_tag="WM704_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFA9340473.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1356..2129
                     /locus_tag="WM704_001035"
     CDS             1356..2129
                     /locus_tag="WM704_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFA9340474.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(2876..3460)
                     /gene="rnhB"
                     /locus_tag="WM704_001036"
     CDS             complement(2876..3460)
                     /gene="rnhB"
                     /locus_tag="WM704_001036"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFA9340475.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(3528..5423)
                     /gene="mnmG"
                     /locus_tag="WM704_001037"
     CDS             complement(3528..5423)
                     /gene="mnmG"
                     /locus_tag="WM704_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFA9340476.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYAPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAIPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(5596..7023)
                     /locus_tag="WM704_001038"
     CDS             complement(5596..7023)
                     /locus_tag="WM704_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9340477.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            7182..8174
                     /gene="pdxA"
                     /locus_tag="WM704_001039"
     CDS             7182..8174
                     /gene="pdxA"
                     /locus_tag="WM704_001039"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFA9340478.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(8404..11163)
                     /locus_tag="WM704_001040"
     CDS             complement(8404..11163)
                     /locus_tag="WM704_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFA9340479.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     VPAAETSEKRSTANRSGSRANNRRRNPRRSKREGSKIEVREAAGKTAGQKARADKAET
                     RNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            11656..12648
                     /locus_tag="WM704_001041"
     CDS             11656..12648
                     /locus_tag="WM704_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFA9340480.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
     gene            12703..12803
                     /locus_tag="WM704_001042"
                     /pseudo
     CDS             12703..12803
                     /locus_tag="WM704_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            12799..13971
                     /gene="lpxB"
                     /locus_tag="WM704_001043"
     CDS             12799..13971
                     /gene="lpxB"
                     /locus_tag="WM704_001043"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFA9340481.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
     gene            13979..14137
                     /locus_tag="WM704_001044"
     CDS             13979..14137
                     /locus_tag="WM704_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340482.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            14183..14497
                     /locus_tag="WM704_001045"
     CDS             14183..14497
                     /locus_tag="WM704_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340483.1"
                     /translation="MFHLFFVNFYFRQLCPTPNYTDWVKIKFKQFSYLKFIYGYATEN
                     QDKDIDNTLELGELKQDDEILDYGGALALIRRRYNLPTSFIIDIVCREIELEFLDQES
                     FN"
     gene            complement(14611..15420)
                     /gene="dapB"
                     /locus_tag="WM704_001046"
     CDS             complement(14611..15420)
                     /gene="dapB"
                     /locus_tag="WM704_001046"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFA9340484.1"
                     /translation="MIPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(15430..15807)
                     /locus_tag="WM704_001047"
     CDS             complement(15430..15807)
                     /locus_tag="WM704_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFA9340485.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            15965..16468
                     /gene="fur"
                     /locus_tag="WM704_001048"
     CDS             15965..16468
                     /gene="fur"
                     /locus_tag="WM704_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFA9340486.1"
                     /translation="MIVGKQAEIAYYSVYYFTGILNIMEKFSNIAQLKDSGLKVTGPR
                     LKILDLFEKHAEEHLSAEDVYRILLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGK
                     AVYELDKGDHHDHIVCVKCGEVTEFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSD
                     CQAKGKR"
     gene            16538..17263
                     /locus_tag="WM704_001049"
     CDS             16538..17263
                     /locus_tag="WM704_001049"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFA9340487.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
BASE COUNT         3396 a         4297 c         5498 g         4109 t
ORIGIN      
        1 cgggcggcaa ggcggtgcgc ggtataatgc ggcctgcttc ggaagagggg ggacggcgat
       61 gtttgtgaac gagaaatatc cttatgcgac cctgtttgcg gggctggtgt ttttgacgct
      121 gccgtttgcg ttggcggtgc atgatgcctt tgcatgtgcg ttcggacggg cggggctgct
      181 ggtgtcggtg tcggccggcg gattcggccg gcgtggcggt tgggacggca ctgtttggtt
      241 tgtgttcggt gtgtttgcgt ttttgaatgt ggttgtgtcg gcgggcctga cgaaactggc
      301 gtacaaaaag atgatgcggc ggcattcgcg ttacgcgctg tttctgtcgg gcgtggcggc
      361 ttgcgcggcg gtggcttgga ttttcaagct gttgttgggc agtgccgcct tgggcgggct
      421 gagcggggag gcggtgtcgg aatatgcgtt tgccgtgtgg ctggtgtcta tgctgacgct
      481 gccgaaacgc ctgacgcgcg cgccggtgca gcctgtggtg ttccacagga aaaaatagga
      541 cggaacggaa atgccgtctg aaacccgata cgcggtttca gacggcatgt ttttccgcta
      601 acattacgcc tgaatatgga caggaagcag atatggaacg taaagaacgc ctgcgtgcag
      661 gcattgccgc gatggggccg gatatttcgg aaacggcgca ggacaggctt ttggcctatg
      721 tggatttgtt gaaaaagtgg aacaaaacct acaatctgac cgccctgcgc gacgaggaaa
      781 aaatgattgt ccatcatctt ttggacagcc tgacgctgct gccctatatc gaaggcgcac
      841 agacgatgct ggatgtcggt tcgggcggcg gccagcccgg cattccggcg gcggtgtgcc
      901 gtccggatgt gcagataacc ttgttggatg cgaatacgaa gaaaacggct tttttgcggc
      961 aggcggctat cgagttgggg ctggacaatg tgcgcgtggt gtcggggcgc gtggaggcgg
     1021 tttcggacgt gcgtgccgac gtggttacca gccgtgcgtt tgcggaactg gcggattttg
     1081 tgtcgtggac ggcgcacctg ttgaaagacg gcggctactg ggcggcgatg aagggcgtat
     1141 atccgcaggg ggaaatcggc cgcctgccgc aggatgtgtg cgtcgaaaaa gtccaaaggc
     1201 tcgacgtgcc gggcttggat gcggaacgcc atatcgtcat cctgagcaag cgttgagcgc
     1261 gcttcagacg gcatgaatac cttttttgtg cggataaagg taaaattccg cactgttttt
     1321 ctttttttca acatcagacg ggacacgggc gggacatgag tgcgaacatc cttgccatcg
     1381 ccaatcagaa gggcggtgtg ggcaaaacga cgacgacggt aaatttggcg gcttcgctgg
     1441 catcgcgcgg caaacgcgtg ctggtggtcg atttggatcc gcagggcaat gcgacgacgg
     1501 gcagcggcat cgacaaggcg ggtttgcagt ccggcgttta tcaggtctta ttgggcgatg
     1561 cggacgtgca gtcggcggcg gtacgcagca aagagggcgg atacgctgtg ctgggtgcga
     1621 accgcgcgct tgccggcgcg gaaatcgagc tggtgcagga aatcgcccgg gaagtgcgtt
     1681 tgaaaaacgc gctcaaggca gtggcggaag attacgactt tatcctgatc gactgcccgc
     1741 cttcgctgac gctgttgacg cttaacggct tggtggcggc gggcggcgtg attgtgccga
     1801 tgttgtgcga atattacgcg ctggaaggga tttccgattt gattgcgacc gtgcgcaaaa
     1861 tccgtcaggc ggtcaatccc gatttggaca tcacgggcat cgtgcgcacg atgtacgaca
     1921 gccgcagcag gctggttgcc gaagtcagcg aacagttgcg cagccatttc ggggatttgc
     1981 tttttgaaac cgccatcccg cgcaatatcc gccttgcaga agcgccgagc cacggtatgc
     2041 cggtgatggc ttacgacgcg caggcaaagg gtgccaaggc gtatcttgcc ttggcggacg
     2101 agctggcggc gagggtgtcg gggaaatagg tcaatccaaa tcgggctgcc cgtgccttta
     2161 tgctgtttgg ccgggtgcgt taatatggcg gattaaaata aaaatactta atatcgtcat
     2221 ttatagcgga ttaacaaaaa ccggtacggc gttgccccgc cccggctcaa agggaacgat
     2281 tccctaaggc gcccaagcac caggcgaacc ggttccgtac tatccgtact gtctgcggcc
     2341 cgccgccttg tcctgatttt tgttaatccg ctatatcgtc attcccgcaa aaacaaaaaa
     2401 atcaaaaaca gaaaactgaa atatcgtcat tcccgcgcag gcgggaatct aggtctgtcg
     2461 gtacggaaac ttatcgggaa aaacggtttt tccaaccctg agactccgga ttcctgtttt
     2521 cgcgggaatc cggttttttg agtttcagtc atttttgata aattcttgca gctttgagtt
     2581 tctagattcc cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa
     2641 aacggaaacc ggacggattg gattcccgcc tgcgcgggaa tgacggattt taggtttttt
     2701 tgattttctg tttttcgcgg gaatgacggt tgggaagttt cccgaaactt aaaaacaact
     2761 gaaacccaaa aaactaaatt accgcctgcg ggggaatgac ggttcgggtt ctttctcttt
     2821 gaagttgcga tgctggaaat gccgtctgaa gttcagacgg catttttgtg ccggtttaaa
     2881 acaaggcctg ctgcgcgagc aggtttctga cgggggcgaa gtcgcggcgg tgttcgggca
     2941 gcacgccgta ttgtttgagg gcttccagat gctgctttgt gccgtaacct ttgtgtttgt
     3001 caaaaccgta ttggggacgg cgttgcgcca gtgcgtacat ttccgcatcg cgtgcggtct
     3061 ttgccaaaac ggatgcggcg gagatttcga tgattttgct gtcgcctttg acgactgctt
     3121 cggcagggat gcctaaatgt tcgggaatgc ggttgccgtc gatgaatatt ttttcgggac
     3181 gcgcagccaa accgtaaacg gcacgtttca tggcgagcat ggtggcgtgc aggatgttga
     3241 ggcttgcgat ttcttcgggc gtggaggcgg cgacgtgcca cgcgaccgcc tgttctttaa
     3301 tcatttcggc aagcgcgtcg cgttttttct cgctgagttt tttggagtcg gtcagtccgg
     3361 gcaggtcgaa tgtttccgga aggatgacgg cggcggcaaa cacgctgccg accaaaggtc
     3421 cgcgtcctgc ctcgtccacg ccggcggtca gtatgtgcat gatgtttcct gtcgggatgg
     3481 tacgatgccg tctgaaaatg gtttcagacg gcatcgtacc gatgtgttta tttcgcgtct
     3541 ttaaacccgc gcttcaaatg caccatcagc agtgcgacgg cggcaggcgt aacgccggaa
     3601 atgcggcttg cctgcccgac ggtttcgggt ttgtgctggt tgagcttttg ttgcacttct
     3661 gccgacaagc ctttgactct gccgtaatcg atgccgtcgg gcagttttaa ggtttcgatg
     3721 tcgcggcggc tgtcgatttc ttcgttttgg cggtcgatat agccttggta tttgacttgg
     3781 atttcgactt gttcgaccac ttcggcggag aggcgttcag acggtatcgc gccttcgagc
     3841 gtcatcagcg cggcgtaatc gaggttcggg cggcgtaaca aatcgtgcag gttggcttcg
     3901 cggctgagtt tttgtccgaa cacgcggatt tgttcgtctt cggcgagttt ttgcggcgcg
     3961 taccacgttg ttttcaaacg ttggatttcg cgttcgacgg cttcgcgttt ttcgttgaac
     4021 atgcgccatt gcgcttcacc caccaagccg attttgtagc cgtcttcggt caggcgcata
     4081 tcggcgttgt cttccctgag ttgcaggcgg tattcggcgc ggctggtaaa catccggtaa
     4141 ggttcgttca ggcctttggt aatgaggtcg tccaccaata cgccgaggta ggcttgttcg
     4201 cggcgcagca ggagcggatc ttgtccgcgg acgtattgca cggcgttcgc gcccgccagc
     4261 aatccttgcg cggcggcttc ttcatagcct gtcgtgccgt tgatttgccc ggcgaaaaac
     4321 agtccctcaa tggttttggt ttcgaggctt gctttgaggt tgcgcggatc gaagtagtcg
     4381 tattcgatgg cgtagccggg gcgcaggata tgggcgtttt caagaccctt catactgcgc
     4441 acgagcgcga tttggatgtc gaacggcagg ctggtggaga taccgttggg gtagtattcg
     4501 tgcgtggtca gaccttcggg ttcgaggaaa atctggtggc tgtctttgtc ggcgaagcgg
     4561 ttgattttgt cttcgataga cggacaataa cgcggaccca cgccttcgat tttgccggta
     4621 aacatcgggc tgcggtcgaa gcctgagcgg atgatgtcgt gggtttgcgt gttggtatgc
     4681 gtaatccagc aggacacttg gcgcgggtgc atttcggcgt tgccgcgcac ggacatgacg
     4741 ggaacgggcg tgtcgccggg ctgttcggtg agttgggaaa agtcaatcgt gcgtccgtca
     4801 atacgcggcg gcgtgccggt tttcagacgg ccttgcggca gcttcaattc gcgcaaacgt
     4861 ccgccgagcg atttggcggc tgggtcgccg gcgcggccgc cttcgtagtt ttccaaaccg
     4921 atgtggattt tgccggacaa gaacgtgcct gcggtcagca ccacggcgcg tgctttaaac
     4981 tccgcgccca tcgcggtgat cacgccgctg atgcgttcgc cctcgagtgt tacgtcttcg
     5041 acggcttgtt ggaaaaggtc gaggttttct tggttttcca acatttcgcg gatggaggct
     5101 ttatacagga tgcggtctgc ctgcgagcgc gtggctcgta ctgccgcgcc tttgctggcg
     5161 ttcaggcggc ggaattggat acctgatttg tcggttgcca acgccattgc gccgccgagc
     5221 gcgtcgagtt cgcgtaccaa atgccctttg ccgatgccgc cgatagaggg gttgcacgac
     5281 atttgtccga gcgtttcgat attgtgtgtg agcaaaagcg tctgcgcgcc catacgggcg
     5341 gcggcaagcg cggcttccgt gccggcgtgt ccgccgccga cgacgatgac atcgtaggtt
     5401 ttggggtaaa tcatgtgggt catgtgtgta ttgcctatcg gtttttcaga cggcattcca
     5461 agcaggatgc cgtctgaaaa acgaaacgga ttcaaaagta aagggttggg attgtacgcc
     5521 tgttcgccct gtttttacag tgcgcggaaa gggaaaagcc gcttcgcggg gaagcggctc
     5581 cggtaagggt gggatttacc aaacatcgga tttgatgcgg cgtttcaggc ccggatgttc
     5641 ggaaagtttg aactcggggt ctttgcccat tttcagcttg gcggtgtaat cgcgcagcag
     5701 cataaacgcc aatggcgaga gcagcaggat ggcgacgagg ttgatccacg ccatgatgcc
     5761 catcgccata tccgccatat cccagaccaa aggcacattg gcaaccgcgc cgaaatagac
     5821 ccacgccaaa accagcatac ggaaaacggc ggtaatcagc caatggcttt tgatgaattg
     5881 gacgttggac tcggcatagg catagttgcc gataacggtg gaaaaggcaa acataaacag
     5941 gatgacggcg aggaaacccg cgccccattg ccccacttgg ctgacaatcg ccgcctgcgt
     6001 cagcgccgca ccgctcaaat cgccataagg ctgttggtaa atcaagatga tgaaggcggt
     6061 gcaagaacaa acgatgatgg tatcgacaaa cacgcccagc atttgaatca taccttgcga
     6121 aacagggtgt ttcacttcgg cggcggcggc ggcgttcggc gcggaaccca tacccgcctc
     6181 gttggaatac aggccgcgtt tgatgcccat catcatcgtt tgcgaaatca gaccgccgag
     6241 taagccgcct gctgccgcgt cgaatttgaa cgcgcccgaa aaaatctgac cgaacacgtc
     6301 cggaatcatc ggaatattgg tcaaaatgat gaaaagcgcg ataaagaggt acaaaaccgc
     6361 catcaggggc acgacgattt ccgccgcttt ggatatgcgc ctgatgccgc cgaagataat
     6421 cggcgcggtt aaaatcacca gggcgacgcc gacataatga ggctcccagc cccacgccgc
     6481 tttgacggta tcggcgatgg tattggtctg aaccgcttca aacacaaaac cgaaacagaa
     6541 aatcaggctc agggcgaaca acacgcccag ccatttctgc cccagaccgt gcgtgatgta
     6601 gtaggcaggg ccgccccgga aatggtggtt gtcgcagtcg cggactttaa agagctgcgc
     6661 cagcgaagat tcgacaaacg ccgaactcat gccgattaag gcggttaccc acatccaaaa
     6721 caccgcgccc ggcccgccga ctttgatggc gattgccacg cccgcgatat tgcccacgcc
     6781 cacgcggctg gcaaggccgg ttacaaatgc ctgaaacggc gtgatgccgt gagggtcgtc
     6841 cccctgtttg cggccgccga gcatttcttt gatgctgcgc ccgaacaggc ggaattggac
     6901 aaagcccgtg gttacggtga agaaaagccc cgtacccaaa agcatataaa ccaagtatga
     6961 ccacatcgga tcgttgatgg cgccgaccca gccgtgcagc cattcggtaa agttctcgtt
     7021 catatcgctt ccttaaagtt gaaacccgca catatggcgg tatgcaagca gggtttaaat
     7081 tttgtaaacg cccattctag cagattgtca acaaaatcag aaaaatttac atcgccgcgc
     7141 gcctgtggcg ttagaatcgc gttttgtttg gagcaaacac gatgaaacag cctgtttttg
     7201 ccgttacttc cggcgagcct gccggcatcg gccccgatat ttgtttggac ttggcgtttg
     7261 cacgcctgcc ctgccgctgc gcggtattgg gcgacaaaca cctgttgcgc gcgcgcgccg
     7321 aagccttggg caaaagcgtc gtcctgcgcg acttcgatcc ggaatcaggc ggcgcgcacg
     7381 gagaaggcgg gctggaagtg ctgcacatcc ctgccgccga agcggttgag gcagggtggc
     7441 tcaatcccgc caacgccgcc tatgtgctgc aacttttgga caccgcgctc gcaggcattt
     7501 cagacggcat tttcgacggc atcgttaccg cgccgctgca caaaggcatc atcaacgccg
     7561 cgcgcgcaag cacaggtttt ttcagcggac acaccgaata tctggcggaa aaaagcggca
     7621 cggggcaggt cgtgatgatg cttgccggca aaggcctgcg cgtcgccctc gtaacgaccc
     7681 acctgccgct gaaagacgtt gccgccgcca tcacgcaacc gctgattgaa agcgtcgcac
     7741 gcattttgca tcacgactta aaacacaaat tcggcatcaa aaatcccaaa atcctagtcg
     7801 ccgggctcaa tccccacgcc ggcgaaggcg gacacctcgg acacgaagaa accgacacca
     7861 ttatccccgc gctcgaaaac ctgcgccgcg aagggataaa cctcgccggc ccgtatccgg
     7921 cggacacatt gttccaaccg tttatgctcg aaggtgcgga tgccgtattg gcgatgtacc
     7981 acgaccaagg gctgcccgtg ttgaaatacc acagcttcgg gcagggcgtg aacatcacgc
     8041 tggggctgcc ctttatccgc acctccgtcg atcacggcac cgcgctcgat ttggcggcaa
     8101 ccggcagggc ggactccggc agcctgataa ctgccgtgga gaccgccgtc gagatggcgc
     8161 gcggcagcct ttaaagatga taaaagaccc gtcattcccg cgcaggcggg aatccggacc
     8221 ttcgggcggc ggcaatattc aaaggttatc cgaaagtttg aggttctaga ttcccgtttt
     8281 cacgggaatg acgaaaggtg gcgggaatca cgggaatggc gaagtttcag gcggtattgc
     8341 aggtatccga aaccatgcaa aaagaggttc tgcggaacag aacctctttt tgccgccgtc
     8401 ggttcagcct tgccgggttt cgacttggat catttcttcg gcagggacgg ttgcggcttc
     8461 agacggtttt ggctgttcgg aacggcgcaa accgcgtccg gcttggactt cgggttgtgc
     8521 cgcccatgcc ttcaatgcgg cagggtcggt ttcgatcagg acgagtccgc cggtttgtgc
     8581 ggtttcccgt gcctgttccg ccgcagccgt aaaggttgcg gtttcagacg gcatttcctg
     8641 tgctgcggct ttcggtgccg cgccttcggg caggatgtcg gcggcggcac ggcggatttt
     8701 ttccgccgca tcataaatca gtgcgtcgcc gtttgaaacg gcggaagatg ccgtctgaac
     8761 gggtgtggcg atgagcggat cggcaatgct gacggtaatc ggcgcgtttg cgtcggtttc
     8821 gccgaaaacg tgcgcggcgg cggaacggac tttgtcggcg gtgtcgtgaa tattcaggta
     8881 ctgctcgatt ttggcggcag acgggatatt gcgttttttg ccgttttgac ggcggtcgcg
     8941 ctgattgttg cgttcgcggc gttctttggc atcgcggctg tcgcgttcgc ggcggttgcg
     9001 ttcggatttg ggcttgctgc ctttgtcttc tgcggtatac ggttcgggcg gcgtgttttc
     9061 cgctgtctga acggctgttt cggcaacggc ggcggtttcc ggtgcggttt gaccacgttc
     9121 gttgcggctg ccgttgcggc ggcgttttcc ggtttgacct tcggtttcgg gcggtgcggc
     9181 atctgcaacg ggtgcggcag gctgtacgtt gcggctttgg atttccgctt cgttggcgcg
     9241 ttcggtggca cggtcgccgc gttcgttgcg gcggcggttg ccgttgttgc gcgtttcggc
     9301 tttgtcggcg cgcgctttct gtccggcagt tttgcccgcc gcttcgcgga cttctatttt
     9361 gctgccttcg cgtttgctgc ggcgcgggtt tcggcggcgg ttgttggcgc ggctgccgct
     9421 gcggtttgcc gtgctgcgtt tttcggaggt ttcggcagcg ggtacggctt gggtttcgct
     9481 gccgccgaaa atgcgtttga gccatgcttt gaagctgtcc caccaggagg tttttttctc
     9541 gggggcggca gtcggggcgg ggctggtgtg gcgcacgcct ttgacggcgg gttcggggcg
     9601 ggcggctttg gctttttcgc cgccgaaagg tttggcggat tcgtcttctt caggctcggc
     9661 aacgcgcttg tagctcggtt cgccgtcttc ttctacgtcg tcggtgcgga tgcggttgat
     9721 ttcgtagtgc ggattttcga ggtggatgtt cggaatcagg acgacgttca catccaaacg
     9781 ctcttccatt gcaaacagct cggcgcgttt ttcgttcagc aggaaggtgg cgacatcgac
     9841 gggcacttgg gcgcgcactt cgccggtgtt gtccttcatc gcttcttcct gaaccatgcg
     9901 caaaacgtgc agggcggtgg attcgatgcc gcggatcacg ccggtgccgg cgcagcgcgg
     9961 acaggcggcg tggctgcttt cgcccaaagc gggtttcagg cgttggcggc tcaattccaa
    10021 aagtccgaaa cgggagagtt tgcccatctg tacgcgggcg cggtcttttt tgagtgcgtc
    10081 gcgcaggacg ttttccacat cgcgctggtg tttggggttt tccatgtcga tgaagtcgat
    10141 gacgaccaag ccgcccaagt cgcgcaggcg catttgtcgg gcgacttctt cggcagcttc
    10201 catattggtt ttgaaggcgg tgtcttcgat gtccgcgccg cgtgtggcgc gtgcggagtt
    10261 cacgtcgatg gagacgaggg cttcggtgtg gtcgatgacg atcgcgccgc cggagggcag
    10321 gctgacgctg cgcgagaacg cgctttcgat ctggtgttcg atttggaagc gggaaaacag
    10381 cggcgtgtgg tcttcgtaga gtttcagacg acctgcgttg cccggcatga cgtagctcat
    10441 gaactcggca acttggtcgt aaacttcttg attgtccacc aaaatttcgc cgatgtcggg
    10501 acggaagtag tcgcggatgg cgcggatgag caacgagctt tccataaaga gcaggtaggg
    10561 gtcgtgatgc gcttttcctg cttcttcgat tgcctgccag agttgtttga ggtagttcaa
    10621 atcccattcc aactcttccg cgctgcggcc gatgccggcg gtacgcgcga tgatgctcat
    10681 gccgttcgga atgtcgagtt gcgccatcgc ggctttgagt tcttgacgct cttcgccttc
    10741 gatacggcgg gatacgccgc cgccgcgcgg attgttcggc atcaatacca gatagcgtcc
    10801 ggcgaggctg atgaaggtgg tcagcgccgc gcctttgttg ccgcgctcgt ctttttcgac
    10861 ttggacgatg acttccatgc cttctttgag cacgtcctga atgcgcgcgc gcccgccttc
    10921 gtagccgagg aaatacgaac gcgatacttc tttaaacggc aaaaagccgt ggcggtcggt
    10981 tccgtaatcg acgaaacacg cttccagcga cggctcgatg cgggtaatga tgcctttgta
    11041 gatattgcct ttgcgctgtt ctttgcccag cgtttcgatg tccaaatcca gcaggttttg
    11101 tccgtcgacg atggcaacgc gcagctcttc ggcctgcgtt gcgttgaata acattctttt
    11161 catgatcacc tcgtggacag gcggcattcg ggcgcgccgg tccggttcgg cattccgtaa
    11221 ggctgggttt ccgatgtctt cggataaaac cggtaatcag tttttgagtt gaaaatccgc
    11281 agggatgcac gttccgggga accgtgtgcg gaagggtcgg ataaagaagg ctataaagat
    11341 cgatgcggcg gtctgcctgc cgcgttccga acgctgcggt cggaaaaatg ggcgccggct
    11401 tcttcttgtt atcgtgatgc ctgtgttttc gggcggtttg cgttcggggc ttggggctcc
    11461 gctgccgtct tacttccgcg ccgaaacggc aaaatcaatt caaacttgac tacgttctgc
    11521 gcctgccggc tgggaacagg cgcagggaaa atgctttgcg gagtgcgttt ttaatataaa
    11581 attccgtttt aaagtaaacc gtttcagggg gcgcggcgga cgcgctttct gctgaaacgg
    11641 atgttcggat tatagatgaa aacgcacgaa ataagcaaag gttcggtcag cctgataggg
    11701 gttgccgaac atgaggcggg tcaacgcctt gataactatc tgataaaaat cctcaagggc
    11761 gtgcccaagg gctatatcca ccgcattatc cgcgccggcg aggtgcggtt gaacaagaaa
    11821 cgctgcaaac ccgacagccg tattgcggag ggagatacgg tgcggattcc gcctgtgcgc
    11881 gtggcggaga aggaaatgcc gtctgaaagg cgtgccgccg taccggcgcg tgcgtttgag
    11941 gtggtgtatg aggacgacgc gctactggtg gtcaataagc cttccggtgt ggcggtacac
    12001 ggtggcagcg gcgtgagttt cggcgttatc gaacagttgc gccgcgcccg tccggaggcg
    12061 aagtatttgg agttggtcca ccgtttggac aaggatacga gcggcttgtt gatggtggcg
    12121 aagaaacgca gcgcgctcgt caaactgcac gaagctatcc gtaacgacca ccccaaaaaa
    12181 atctaccttg cgctgggggt gggcaaactg ccggacgaca atttccatgt caaactgccc
    12241 ctgttcaaat ataccggcgc acaaggcgaa aagatggtac gcgtcagcga ggacgggcag
    12301 tcggcgcata cggtgttccg tgtgttaagc cgtttctcag acggcatttt gcacggtgtc
    12361 gggctgtcgc acctgacttt ggtgcgggca acgctgaaaa cgggccgtac gcaccaaatc
    12421 cgcgtgcatc tgcaatctca aggctgtccg attgcgggcg acgaacgcta cggcgattat
    12481 caggcgaacc ggcgtttgca gaaattgggt ttgaagcgca tgtttttgca cgcgtccgag
    12541 ctgcacctga accatccgct cacgggtgag ccgctggtgt tgaaggcgga gccgccgccg
    12601 gatttggcgc agtttgcggt gatgttggaa aacgggacga aaatgtgaac cccgatgccg
    12661 tctgaaggct tcagacggca tcggggcgtg aaagtatagt ggattaacaa aaatcaggac
    12721 aaggcggcga agccgcagac agtacagata gtacggcaag gcgaggcaac gccgtaccgg
    12781 tttttgttaa tccactatat gtggggacag acgaatatgg ttgataaaaa aagccctttg
    12841 attgccgtca gtgtcggcga agcgtcgggc gacctgttgg gggcgcacct gatacgcgcc
    12901 atccgaaagc gttgcccgca ggcacggtta accggcatcg gcggggaact gatgaaggcg
    12961 gaaggtttcg agagccttta tgatcaggag cggctggcgg tgcgcggctt tgtcgaagtg
    13021 gtcaggcggc tgccggaaat tttacggata cgcagggagc tggtacggga tttgctgtcg
    13081 ttgaaacctg atgtctttgt cggtatcgat gcgcccgatt ttaatctggg tgtggcggaa
    13141 aagctgaaac gggcgggcat tccgaccctg cattatgtca gtccgtcggt ttgggcgtgg
    13201 cggcgcgaac gcgtgggcaa aatcgtgcat caggtcaacc gcgtgctgtg cctgttcccg
    13261 atggagccgc agctttatct cgatgcgggc ggacgtgcgg agtttgtcgg tcatccgatg
    13321 gcacagctta tgcccttgga agacgaccgt gaaacggcgc ggaaaacttt gggcgcggat
    13381 gtcggcatcc ccgtattcgc cctgctgccc ggcagccgcg tcagcgaaat cgactatatg
    13441 gcgccggtgt tttttcagac ggcattattg ttgttggaac gctatcccgc cgcccgcttc
    13501 ctgctgcctg ccgcaacgga ggcgacgaag cggcgtttgg cggaagtttt gcagcggccg
    13561 gagtttgccg gattggcgct gacggtaacc gacagacagt ctgaaacggt gtgcagggcg
    13621 gcggatgcgg tgctggtaac gagcggtacg gcgactttgg aggtggcgtt gtgcaagcgt
    13681 ccgatggtca tcagctacaa gatttcgccg ctgacctatg cttatgtgaa acgcaaaatc
    13741 aaagtgccgc atgtcggcct gccgaatatc ctgttgggta aggaggccgt gccggaatta
    13801 ttgcaatctg aagcaaaacc ggaaaaactg gcggcggcat tggcggactg gtacgaacac
    13861 cccgataagg ttgccgcgct gcaacaggat ttcggggcgt tgcacctgct gttgaaaaaa
    13921 gatacggcgg atttggccgc gcgtgcggtt ttggaagagg cgggatgttg agcggttaat
    13981 ggattatttt cccgaagcag cacgtattac aaaaaaaggg ggagaaattg tgattaatgg
    14041 cacatcaaac aataagtatt taagaggaat tccaaatgaa acagaactgg cccgaatggg
    14101 attaaggtta aaatataatg gttcgttaac tgattaattt tattatatat gattatagtt
    14161 tatactaata cgcttacgta ccttgtttca tttgttcttc gtaaatttct attttaggca
    14221 attgtgtcct acaccaaatt acacagactg ggtaaaaatt aaattcaagc agttcagcta
    14281 tttgaaattt atctatggat acgctacgga gaaccaagat aaagatatcg ataatacctt
    14341 ggagcttgga gaattaaagc aggatgatga aatcttggat tatggaggtg cactggcatt
    14401 aataaggcgt aggtataatc ttccgaccag ttttatcata gatatagttt gccgggaaat
    14461 agagttggaa tttttagatc aggagagttt caattaaacg agccgtagct tgtcatgctg
    14521 cacaagcaac tttattgata tgccgatacg aagcctgtcg gcaaaatgcc gtctgaacaa
    14581 tatcttttca gacggcattt tgtatggggg ttaacggttg ttcagcccga gtacgtcctg
    14641 catatcgtac aaacccgttt tgccgtttac ccaaactgcg gcgcggacgg caccggcggc
    14701 aaaggtcatg cggctgccgg ctttgtgggt gatttccacg cgttcgccgt cggtggcgaa
    14761 gagggcggtg tggtcgccga ctatgtcgcc tgcgcggacg gtggcaaagc cgatggtgga
    14821 aggatcgcgc ggaccagtgt ggccttcgcg gccgtaaacg gcgcattgtt tgaggtcgcg
    14881 gccgagcgcg ccggcgatga cttcgcccat tcgtaatgct gtgccgctgg gggcatcgac
    14941 tttgtggcgg tggtggcctt cgatgatttc gatgtcgtag ccttcgttga ggacgcgggc
    15001 gacggtgtcg aggatgtgga aggtgaggtt gacgccgacg ctgaagttgg cggcgaaaac
    15061 gatgcctgtt ttttcggcgg cggcgcggat ggcggctttg cccgcatcgt cgaagcctgt
    15121 tgtgccgatg atgatgttga cttgtttttc aacgcatttt tgcaggtgct tgagggtcgg
    15181 ctcggggcgg gtgaagtcga tgagtacgtc gctttgggcg agaacggcgt caacgtcgtc
    15241 tgaaatggcg atgccggttt tgagtccgac ggcgtagccc gcgtccagcc cgagggcttc
    15301 tgagcctgag tgttcgagcg cgccggaaag gacggtgtcg ggatggttgt tgacggcttc
    15361 gacgaggacg cgtcccatgc ggccgtttgc gccggcgatg gcgattttga gcggtatcat
    15421 gtgtgttcct tattgtttgt ctgcgttttg tttcgcgcgg agggcttcgg cggcattttg
    15481 gagggcgtcg ccttcggtgc ggacgagtac gccgttttca aaatagacgg tcaggttgct
    15541 gcgttctttg atgatgccgt tgcgggaggt gttgaaggta tagtcccagc ggtcggtatg
    15601 gaaagcgtcg cgcagtatgg ggctgccgag caggagcagg acttggtctt tggtcatgcc
    15661 ggggcgcagg gcggcaacgg cgcgcggttc gagttcgttg ccttggatga ttttgagttt
    15721 gtaggaggga aacagcgaga cgcgttcgac gctgcacgcg gtcaggctga acagggcgga
    15781 aagggcgagg atgagggttt tgttcacggg agaacctttc tgtgcaaatc gggatgtcta
    15841 gtgtagcact gcttgaatat tttataaaag cgaacgataa tcatacgctt aagcggtatt
    15901 ttaccctgtc tgtgtccgac tgcccgtccg ccgttgcggt tttgctattg cgaactgcta
    15961 tggtgtgata gtgggcaagc aggctgaaat tgcgtattat agcgtctatt attttacagg
    16021 gatattgaat attatggaaa aattcagcaa cattgcgcaa ctgaaagaca gcggtctgaa
    16081 ggttaccggc ccgcgtttga agattttgga tttgttcgag aagcacgcgg aagagcattt
    16141 gagtgcggaa gatgtgtacc gcattctgct ggaagagggc gtggaaatcg gtgtggcgac
    16201 gatttaccgc gtgctgacgc agttcgagca ggcgggcatt ctgcaacgcc accattttga
    16261 aacgggcaag gcggtttatg agttggacaa gggcgaccac cacgaccata tcgtctgcgt
    16321 gaagtgcggc gaggtaacgg aattccacaa tcccgaaatc gaagccctgc aagacaaaat
    16381 cgccgaggaa aacggctacc gcatcgtcga tcacgcgctt tatatgtacg gcgtgtgcag
    16441 cgactgtcag gccaagggca aacgttaaat ccggacggtt tgttgttcag acggcattca
    16501 tgattttgga tgccgtctgt gtttttggag aactgccatg cgtattccgc tgcttgcccc
    16561 tgacaattat gcctttcccg atcctgccta tgctttggcc cggtgcgacg ggctggtcgg
    16621 cgtgagcagg gatttggacg cggggcggct gctcgaggcg tatcggaacg gcgtgtttcc
    16681 ttggttttcg cgggacggtt ggtttttttg gtatgcggtc gggccgcgtg cggtaattgt
    16741 tcccgaacgt ctgcacgttc cgcgctcgct ggcgaaaacg ctgcgtaacg gcagctatcg
    16801 ggttgcggtc aacggctgtt ttgcggaagt tgtcgcgcat tgtgcggcag cggcgcgccc
    16861 gaatcaggat gggacttgga ttgcgcccga gtttcagacg gcatatttga agctgcacga
    16921 aatgggacac gcgcattctt tcgagtgcca ttatcccgat gaaaacggta aaacgaggtt
    16981 ggcgggcggc ttttacggcg ttcagatcgg cagggtgttt tatggcgaat cgatgtttgc
    17041 gttgcaaccg gatgcgtcga aaatcgcgtt tgcctgcgcc gtgccgtttt tggcggattt
    17101 gggcgtggaa ctgatagact gccagcagga cacggaacat atgcgccgtt tcggttcgga
    17161 gctgctgccg tttgcggatt ttgccgaacg tctgcggatg ttgaacgccg tgccgttgaa
    17221 agaggaaatc gggcggcgcg aagtggcgtg caaggggctt tgatggcggc ttatgcttcg
    17281 gtcaggttca aatatagtgg
//
LOCUS       DAWTHD010000041        17232 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWTHD010000041 DAWTHD010000000
VERSION     DAWTHD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.041"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(326..772)
                     /gene="smpB"
                     /locus_tag="WM704_001050"
     CDS             complement(326..772)
                     /gene="smpB"
                     /locus_tag="WM704_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFA9340488.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(827..1837)
                     /gene="waaF"
                     /locus_tag="WM704_001051"
     CDS             complement(827..1837)
                     /gene="waaF"
                     /locus_tag="WM704_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFA9340489.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSRFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDNEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(1968..2043)
                     /locus_tag="WM704_001052"
     tRNA            complement(1968..2043)
                     /locus_tag="WM704_001052"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2008..2010),aa:Asn,seq:gtt)
     gene            complement(2234..2986)
                     /locus_tag="WM704_001053"
     CDS             complement(2234..2986)
                     /locus_tag="WM704_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFA9340490.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            3336..3893
                     /locus_tag="WM704_001054"
                     /pseudo
     CDS             3336..3893
                     /locus_tag="WM704_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            complement(3871..4686)
                     /locus_tag="WM704_001055"
     CDS             complement(3871..4686)
                     /locus_tag="WM704_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFA9340491.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTFDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADVAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(5106..6251)
                     /gene="dapE"
                     /locus_tag="WM704_001056"
     CDS             complement(5106..6251)
                     /gene="dapE"
                     /locus_tag="WM704_001056"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFA9340492.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            complement(6364..6984)
                     /locus_tag="WM704_001057"
     CDS             complement(6364..6984)
                     /locus_tag="WM704_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFA9340493.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(7018..7527)
                     /locus_tag="WM704_001058"
     CDS             complement(7018..7527)
                     /locus_tag="WM704_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFA9340494.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            complement(7622..8173)
                     /gene="azu"
                     /locus_tag="WM704_001059"
     CDS             complement(7622..8173)
                     /gene="azu"
                     /locus_tag="WM704_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFA9340495.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(8456..8878)
                     /locus_tag="WM704_001060"
     CDS             complement(8456..8878)
                     /locus_tag="WM704_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HFA9340496.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            9010..11760
                     /gene="secA"
                     /locus_tag="WM704_001061"
     CDS             9010..11760
                     /gene="secA"
                     /locus_tag="WM704_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFA9340497.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            11905..13677
                     /locus_tag="WM704_001062"
     CDS             11905..13677
                     /locus_tag="WM704_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFA9340498.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LGDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            13864..15792
                     /gene="rpoD"
                     /locus_tag="WM704_001063"
     CDS             13864..15792
                     /gene="rpoD"
                     /locus_tag="WM704_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFA9340499.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(15832..16017)
                     /locus_tag="WM704_001064"
                     /pseudo
     CDS             complement(15832..16017)
                     /locus_tag="WM704_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(16183..16398)
                     /locus_tag="WM704_001065"
     CDS             complement(16183..16398)
                     /locus_tag="WM704_001065"
                     /inference="COORDINATES: protein motif:HMM:NF014040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFA9340500.1"
                     /translation="MEADTKTGRLKPVEYKRGKPKPDPGDEIQLCAQGLCLEEMTGQT
                     VSEGALWYMQTRHRVPVVFSDGLRPLQ"
     gene            complement(16368..16832)
                     /gene="cas1"
                     /locus_tag="WM704_001066"
     CDS             complement(16368..16832)
                     /gene="cas1"
                     /locus_tag="WM704_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HFA9340501.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQERKKVAQLPVHSIGHI
                     FCFGNVLVSPFLLGFCGENNVNLAFFTENGCYLGRLQGRQNGNVLLRRAQYRVSEQSP
                     MPIACNIIAAKIRAIRKRARACALSGQCMFWRKNRASAAYWIWWKRIQKQAV"
     gene            complement(16892..>17232)
                     /locus_tag="WM704_001067"
     CDS             complement(16892..>17232)
                     /locus_tag="WM704_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340502.1"
                     /translation="NNTYIFILIAIAVLFLITITLRRITKYQTRYPLVFNYAISGMGH
                     RLSLEKISISQCPQCGGEMKYYNKPIEWENIRYSDGSKKRRVTRTTPALECKRNREHW
                     YKVDPAEDKI"
BASE COUNT         4396 a         4893 c         4170 g         3773 t
ORIGIN      
        1 agcataaaac tgaacaaaaa atgtcattta gttcaaattt tctctattta tttcttattt
       61 attaaaagta aacgtttgct ttttgctatt ttgtcaagcc ggtttgaaaa tgtgtataat
      121 tgccctcgtt atttacaaaa atttcaggaa aaatgaccgc actttaccct tggctaatgc
      181 caatttatca tcaaattgct caaacctttg acgaaggttt ggggcatcat gccgtgctga
      241 ttaaagcgga tgctggttta ggtgtagaac gtttacgcat caggcggcag ccttgcccgt
      301 accgtctgaa gcattgtttc cacaatcagc gcgtatgctt aatcaaccgc tgtttctcgc
      361 gtttccagtc ggcttccttc atactttgtc gtttatcgtg ctgtttctta ccttttgcca
      421 aaccgatttc catcttgatt tttccgcgtg aaaaatgcaa atccagcggc acgatggtgt
      481 aacctgcacg ttcggttttt ccgattaact tattgatttc cgactgtttt aataacagtt
      541 tgcgcggacg tacggcatcc ggtttgatgt gtgtcgaagc cgtaggcaaa gccgtaatat
      601 ggcagccgac cagataaaac gcatcttttt tccaatagat ataactctct ttaagctgta
      661 cgcgcgcggc gcggattgct ttgacttccc agccttccaa gaccaaaccg gcttcaatcc
      721 ggtcttcaat gaaaaaatcg tgaaatgctt ttttattgtt cgcaatagcc ataaacatcc
      781 tatcaatatc cgccgtcaga cggtataaat ccgaaaacag aagccatcat accgcctctt
      841 caaccgcctg cacaatcttc tcgggataca gcctgttgag gcagtcggta tgccccaacg
      901 ggcattcacg tttgaaacag ggcgaacatt ccaagtgcag gctgacgatt ttcgccctgt
      961 cgctcaaagg cggcgtatgc gtcgggctgg aagaaccgta aaccgccact accttcctgc
     1021 ccaaagcagc cgccaaatgc atcagcccgc tgtcgttgca cacgaccgtg tccgccaacg
     1081 acagcaaatc cattgcctgc gacaaatcgg ttttgccgca caaattgaca cacatgccgt
     1141 ctgaaaggca gttgatttct tcggcgattt cattatcttt ttgcgaaccg aacaaccaaa
     1201 cctgccaacc cgcctccgaa tagtgtttgc ccaactcggc aaaatgcctt gccggccaac
     1261 gctttgccgg cccgaattcc gcacccgggc aaaaagccag aacaggcttt ccaagattca
     1321 aaccgaaagt ttcgacagaa atttcccgcc gccgttcatc aatggaaaac tcggggaatc
     1381 tcgaatgccc gtcaaaatct tcctgactct gatgcgcgag agccgtatat cggtccacca
     1441 tcaaaggcag acgttcctta tccagcctgc gtatatcgtt caacagaaaa taacggcttt
     1501 cgccgacata acccgttctt tttccgatgc ccgttgccag cgcgatgact gccgacttca
     1561 aagaacccgg caacacgata accctgtcgt atccgcgccg tcccaactcc ctgccgaccc
     1621 gccaacggcg tttcagctcc aacgcaccgt gtccgaacgg attttcaaga atctcattca
     1681 cttccggcat acgctcgaac accgccatcg accacttcgg tgcgaacaca tcaatcgtgc
     1741 aaccgggatg aagtttcttc aaacggcgga acaagggctg ggtcatcacg cagtcgccta
     1801 tccaactggg agaaataatc aggattttga tagacataaa acgaaaccga aatcagacag
     1861 gcagaatttt accgcgaaac cgttggaaaa cctatctcgc cgcattccga gcgccggacg
     1921 tgcaaatgtg aaaaagcccg aacattcaag tttgggcttc aaaattctgg ctccccgacc
     1981 tgggctcgaa ccagggacct gcggattaac agtccgtcgc tctaccgact gagctatcgg
     2041 ggaatggggc gtattatagc gtccggaaaa aatgtgtcaa tccttaattt tggaaaaatg
     2101 ggcgacaaaa cgacaagcat atgaatcaga aagacattaa gaccgatgcc ttaaaaggat
     2161 tgccgttgta tgaattttca cagccgtcat cacaccatat ttaagcccga tgagccgttc
     2221 tgcatttccc cgcttaaaac aatgccgtct gaacttcgcc gcgttccaaa gccagtaaaa
     2281 actgtttgcg gttcaacccg cccgcgtagc cggtcagttt gccgtcgctg ccgatgacgc
     2341 ggtggcaggg aatcatgaca gacaccttgt tctgcccgtt ggcggcggca acggcgcgga
     2401 cggctttggg gttgcccaaa cgctgcgcct gctccttata actgcgcgtt tcgccgtaag
     2461 gaatcgccaa aagcacgtcc cacgcctgtt tttgaaacgc cgtaccaatc atttccaagg
     2521 gcgtggcaaa ggttttcaga cggcattgga aatacaaatc caattcctga cgcaaaaatt
     2581 gcatccgccc gtcttcccga aacacaaacc gtccgcgcaa ggctttttgg acggcggcaa
     2641 tttcctgttc caaatacttc tgtccgacaa actccagcag gcacaatccc ttaacgccga
     2701 acaccgccaa catctcgccc aaaggcgtgg caatagcggc acacaccagc tcgttcaagc
     2761 tgtcgggata acgtaattcc aacagacgga tggcgcggcg gatacggaca tattcttcag
     2821 gcgcgcagcc gatattgtcc caaaaatccc gctcgaactg cccggcttcg cattccgtca
     2881 gattgggata cggcataacg ccgcactcaa aaacccgggt ttcgaggcaa tggcggattt
     2941 catcccattt tgacggcaga ttgtttaagg aaggcagggt aatcattcgt ttgctccgta
     3001 tccctatcat aaatttgacg gcgaaatccc cgatttccgc cattcccgca cgccggtaca
     3061 ggaacaggct gcggcggaaa ccttgagggt ctggttgcgg caatgcctaa atatacaata
     3121 tttccaaagc atcagggaaa ggaatgtttc aacacacagg acgacacata aagcgtcgcc
     3181 ctatgaaaaa tttcagacgg ccttcaaagg gtcgtctgaa accacgattt ttgcatttgc
     3241 gcattccggc acatcatcca accgtttcgg cacattcctg ccgccgttga cagcctataa
     3301 tgaatccact tattcatcaa gcaaaggaat catctatgca aacccgcatc ctctccgccg
     3361 tactgctggc tttttcaacc gctgcctttg ccgggggcgc attcacgctg caattcgaca
     3421 acccgtccga agacggcggc ttcacgcaaa accagctttt gagcgcgcct tacggcttcg
     3481 gctgttcggg cggcaatgct tcgcccgcgc tgtcgtggta aaacccgccc gcagggacaa
     3541 aaagtttcgt cctgaccgtt tacgataaag acgcgccgat cggtctggac cggatgcact
     3601 gggtggtcgc cgacattccc gccgatgtcc gccgcctgcc cgcaggcatt accgcacaat
     3661 gaggcaggcc gcccaaaggc gcgctgcaaa cccgcacgga cttcggcgcg ccgggctacg
     3721 gcggcgcgtg tcctcccgaa ggcaggaaac accgctacga attcacgctt accgcgctga
     3781 aagtcgccaa gctgccgaac atcaccgccg aatccacacc cgtgctggtc ggtttctcca
     3841 ctaaggcaaa cagcttgggc gaagcgaaat tcacggtcga acaccgacgc tgacctgccc
     3901 cgtgcgccga tatgccgccg gctccgcttc cgagcggacc accgacggta cgcaaccgaa
     3961 cagctctttg aagcgcgcgc tgaaacggga cgggcagtca tagccaaccg cgccggcaat
     4021 ctgcgctacc gaccattgcg taacctgcaa cagcgtcagc gcgcgcatca tgcgtacatc
     4081 cgtcagcagc gtacggaaat tcgtgtcctg ccgcgccaac cgccgccgca acgccgcctc
     4141 gctgcaatta agccgctgcg ccagcattgc cgacgaccag tccgcagcca catcggcagt
     4201 aatcagtttg cggatttgcc gcatcaggtt gacgcctccg tacacggcaa aaccgatgcc
     4261 gtcatgctgc aaccacgcca acacgccgca aagcgcggcc tccgccgcat tgtgcggcac
     4321 ttcttcatcc gccagtaccg cagccgcata atcaaacgcc gcgcccatac gcccggggtg
     4381 cggcagcttc accgcatcgc acactgcctg cgccgtcccg tattgtgcgg cgaacctttc
     4441 tacggtttcc tgttcgaaag caatccattg cgcctgatag aggccgtctg aatcgggaat
     4501 attgatgacg tcaaacgtct gcccgcctgc caagccgaca gcctcacccg ccgcaatcct
     4561 caattcgcgc cccgcccacc gcagcttctt gcatccgcgc cgcacgataa ccacggtcgg
     4621 ttcatatacc gccacgctgc ggtacaaggc ggtatgatgt tgcacgatgc cgcctaaagc
     4681 acccaatcgt tcacgcgtat gaaagtaatt tgacggcacg gttatattcc tcaatgtttc
     4741 aacacacagg gccgcacata aggcgcagcc ctgtttaccc tgatttggaa ggggttactt
     4801 cccaaaaaat aaaccaaaat tctactgccc tgaagggcag ggtttcaacc atattccgtc
     4861 ggtgtgcctg caacttagct ccctcccctg tggggagggt cggagggagg gtaaaacgtg
     4921 gcagatacag acaatatttc cgttgccgtc ccaatgccct ctccctaacc ctctcccacg
     4981 ggagagggaa tggattaccg ttcaaataaa tcgctctaca taaaaatcaa tgcgttatct
     5041 caaactcatg ttgggtctaa tcagattgtc ggacatccat atccgacaac cagcccatct
     5101 ttttttcaga cggccttttc agccaacaac cgcgccaata tcctctcata caccgccgac
     5161 agcttcggaa tatcgtccag ccgcacattt tcgttgattt ggtggatggt ggcattggac
     5221 gggcctaatt cgatgagttc tttcgcaatg gctttaatga agcgtccgtc cgaagtgccg
     5281 ccggtggtgg acaattcggc ctcaacgccg caggtttcgg caatggcggt gcgtgccacg
     5341 tcggtcagtt tgcccgcgtg ggtcaggaag ggctgtcccg aacacgacca ctgcaaatcg
     5401 tattgcacgc cgtgtttgtc caaaatggcg tggacgcgtt gtttcagccc tgtttcggtg
     5461 gactctgtgg agaagcggaa attgaatttg acgttcagct cgcccggaat gacgttggtc
     5521 gcgcctgtgc cgccgttgat attggaaatt tgaaagctgg tcggcgggaa gtattcgttg
     5581 ccttcgtccc aaacttcctg cgtcaactct aacaaggccg gggcaaaagt atgcacggga
     5641 ttcaccgcca aatgcggata ggcgatatgc ccttgcttgc ctttgacggt cagattgccc
     5701 gacagcgaac cgcgccggcc gtttttaatc atatcgccca atttgtccac ggcggtcggt
     5761 tcgccgacga tgcagtagtc aatcaactct ccgcgtgctt tcaacacatc gacgactttg
     5821 gtcgtgccgt ccagtgcatc gccctcttcg tcggaagtaa tcaagagtgc aatgctgcct
     5881 tgatggtcgg gatgttcggc aacaaagcgt tcgcaggcgg taacgaaaca ggcgatgctg
     5941 gttttcatgt ccgccgcgcc gcgcccgtat aatcttccgt cgcgctcggt cggttcgaac
     6001 gggggcgaat cccatttttc aacaggacct gtcggcacaa cgtcggtatg ccctgcaaaa
     6061 cagacgacag gagctttcgt gccgcgtcgc aaccagatgt ttttggtgtc gccgaaatgg
     6121 agttcttcag ccgcaaaacc gattttgtgc aggcgttcgg caagcagttt ttggcaatct
     6181 cggtcgtcgg gggtaacgga cgggcgggaa atcaacgctt tggcgagttc tagggattgg
     6241 gtttcggtca ttttgttcac tttggaaatc aggtcgtctg aaatgtcctg aagctgattt
     6301 tcagacgacc tccgggttgg attggtgtat aagacgggca ttcgtacccg tcagttttct
     6361 gaatcatttg ccgtgacagg cttcgtaaag cggcagtaaa tcttccaccg tttcggctat
     6421 ccattttgct acatcctgct tgcccaaatc gtcgcgctca atgtgtttgc cgatgcagaa
     6481 aaagtcttcg tcgttttgca accttcggtc ggattcgctt tgttgtgcga cggtgcggta
     6541 gtcgtcatat tcgctttccg cgccgtgcca catatcgaat gcggcgtatt tttcggtatc
     6601 gaaatcatcc agccagcggt tgtactcggg cagcgcaatc ggcgacacgt cggctttgta
     6661 gcaatgccag tccaagctga cgctcaggcg gcggcggttg agcagtatcg acaggatggc
     6721 ggcggaattt ttgtattggg cgtatttgaa gtaggcgaag aaatgggcgc gaacctgcca
     6781 gccgttacac cagcgttcga tatgcggcgg ggcgaaaggt tcgctcaaat ctgcggcgac
     6841 ctgctgtatc aactgctgcc atatctgcca gttttccttg tagtcggctt tgatttgcgg
     6901 aatgctctcg ggctggtatt ttttgagctg ggcaaactgg aaaaacggga tattgaacaa
     6961 atcgcagctt ttgggcgtca gcataatata tccttgagac gattgtttca gacggcatta
     7021 tttgcaccgg cgcgccgcca tgatttcgcc gatttcggtc agcttctctt cggggataaa
     7081 cgttttgccc atgtcgaaca aaggctcttc aatcgccaga tgcacatcat atccctccac
     7141 aaagcgtttg aacgcttccg catcggggat ataagcgttg tctgcttcga gtttggcaaa
     7201 ttcggcggaa acagccgccc agttgtcgta cagcccgata tgttggcgca acagctcgtc
     7261 cacgccttca cgggcttgcg gcgcgtattg cagcagcagc gggaaaaagt tttcttcttc
     7321 gtcctcatgg tgcagcggcg cggcaacgtt gaaataccgg gagatttggc ggatggtttg
     7381 caaaacaagc tgattgcagc cgttttcggc gatatagccc gacaacatag caatctggcc
     7441 gcaaaaacgg cgcactttgc cgtggcaggc atacagcatt tcaatcggtt cggcaaaggt
     7501 aacgctttgg gtttcaaacg gattcatgtt ttcgttctca acggggactt ttcaagcagt
     7561 cattttataa taaaacagcc tgcacaaagc aggctgtctg tcttttgaaa ctttaagcgg
     7621 attaatcgac caaagtcact ttgccgttca tcaaagcacc gtgacccggg aaggtgcagg
     7681 caaatttgta gtcgccgtca gccaatttgg caggatccaa agtcagggaa gactcttcgc
     7741 cgccgccgat cagtttggtg tgggcaacaa cgcgcgcatc gtcaggtttg acatagtcgg
     7801 tatcggcagc acctacgccg tctttaaata cgccgtccat gtcttcagct ttggcaatca
     7861 caaggttgtg acccatgctg gctttgggtt gcgtaccggt atgtttcaga gtgatggtga
     7921 actctttaca tgctttgctg acttggatgt ctttggtgtt gaactgcata ttgtcgttgg
     7981 attcgacagt tgccgcacag ttgccggcag caggggcttc ggcagcatct gcaggagcag
     8041 cttcggcggc aggcgcttcg gaagcgggtg cttcaccggc aggagttgcc tcggcagcag
     8101 gcgcggcagg ttcttgagag caggcagcca aaccgataac ggcggcagaa atcagagcca
     8161 gataagcttt catggtaaat ctccaatcga taaaataata ttcggtttta cggaaatcaa
     8221 agtgcaaccg ccattaacaa aaccttgaaa aagattccgc cgtgttgcac aaacagatgt
     8281 ttcggggcgg cattttgcta caaacttcat ttgaaatcaa agcctgtttg caagtttaca
     8341 atcgtttacc caaaaaaagg gcaatcttac cccgaaccta tttctttaat attagggcag
     8401 ccgtcccccg ccgcgcaaat attaacggat atttacaaca tccctgccgg ccgccttatg
     8461 ccccgtactt cctgaccagc tcctccagtg cctccgccaa ttcgggatgc cgttcttcca
     8521 actttgccgc cgccgaatcg aaactctcca acgcagcctt actcaaatgc agggtattgg
     8581 ttttcggcgg tttttccggt tttggaacca gcctgaccga aacagaccgt atcgaagcat
     8641 caagccctgc caactgcggc aataccgacg gggcaatcat cttcaaacgc gatgccgcca
     8701 tattgtttgc cgccaaaagg acaagcctgc cgtcttcgat acatgccgtc tgaaagtggg
     8761 ggtgcaggtt ggcaggcagc agttttttca cggcagcatc caaccgccgc cactgtcccg
     8821 cctgtttcaa aagtccggaa agcagcgcgt cccgcctgcc caactgttcc aaattcataa
     8881 aacatacacc ccaaaagatt gaaataccgc aaacgcgcct ttatttcaga cggcattagc
     8941 actttgcaca aacgcttgtg ttaaaatcgc gttttcgccc cactattata tcaggcgcag
     9001 gaattattca tgctgacaaa cattgccaag aaaatcttcg gcagccgcaa cgaccgcttg
     9061 ctgaaacaat accgtaaatc cgttgccaga atcaacgcgc tcgaagaaca gatgcaagcc
     9121 ctaagcgatg ctgatctgca agccaaaact gccgaattca aacaacgcct cgccgacggt
     9181 cagactttgg acggcatttt gcccgaagcc ttcgccgtct gccgcgaagc gtcccgccgc
     9241 gtactcggta tgcgccactt cgacgtgcag cttatcggcg gtatggtgct gcacgacggc
     9301 aaaatcgccg aaatgcgtac cggcgaaggc aaaaccctgg tcgccaccct cgccgtctat
     9361 ctcaacgcgc tggccggcaa aggcgtgcac gtcgttaccg tcaacgacta cctcgcctcg
     9421 cgcgacgcag gcattatgga gccgctctac aatttcctcg gtctgaccgt cggcgtgatt
     9481 atttcagata tgcagccgtt cgaccgtcaa aacgcctacg ctgccgacat tacctacggc
     9541 acgaacaacg aattcggctt cgactacctg cgcgacaata tggtaaccga ccaatacgac
     9601 aaagtgcagc gcgaattgaa ttttgccgtt gtcgatgaag tggactccat cttgattgac
     9661 gaagcgcgca ccccgctgat tatctccggt caggcagacg acaacatcca gctctaccaa
     9721 atcatgaaca ccgttccgcc ccacctcgtc cgtcaagaga cagaagaagg cgaaggcgac
     9781 tattgggtcg acgaaaaggc acatcaggtc atcctgagcg aaacaggtca cgaacacgcc
     9841 gaacaaatcc tgacccaaat ggggctgctg gcagaaaacg actcgctgta ctcggcggca
     9901 aatatctccc tgatgcacca ccttatggca gcattacgcg cgcactcgct gttccacaaa
     9961 gaccaacatt acgttatcca agacggcgaa atcgtcatcg ttgacgaatt tacaggccgt
    10021 ctgatgtccg gccgccgctg gtccgaaggc ctgcatcaag ccgtagaagc caaagaaggc
    10081 gtggaaatca aacgcgaaaa ccaaaccctc gcctccatca ccttccaaaa ctatttccgc
    10141 ctgtacacca agctctccgg tatgaccggt acggcagata ccgaggcttt cgagttccaa
    10201 agcatctaca acctcgaaac cgtcatcatc ccgaccaacc gccccgtaca gcgcaaagac
    10261 ttcaacgacc agattttccg ttccgccgaa gaaaaattcg aagccgtcgt caaagacatc
    10321 gaggaatgcc acaaacgcgg gcagcccgtc ctcgtcggta cgaccagcat cgaaaactcc
    10381 gaactggtct cccgccttct gcaaaaagcc ggactgccgc acaacgtcct caacgccaaa
    10441 gaacacgaac gcgaagccct gattgtcgcc caagccggca aagtcggcgc aattaccgtc
    10501 gccaccaata tggcgggacg cggtacggac atcgttttag gcggcaacct gaagcaccaa
    10561 accgatgcca tccgcgccga cgaaaccttg agcgacgaag agaaacaggc acaaatcgcc
    10621 gcactcgaaa acggctggca ggcagaacac gataaagtga tggaagcagg cggtttgcac
    10681 atcatcggta cggaacgcca cgaaagccgc cgtatcgaca accagttgcg cggacgttcc
    10741 ggccgtcagg gcgaccccgg atccagccgt ttctacctct cgtttgaaga cccgctgttg
    10801 cgcctcttcg cactcgaccg cgccgccgcc atcctcaacc gcctcgcccc cgaacgcggc
    10861 gttgccatcg agcacaacct gctgacgcgc caaatcgaag gggcgcaacg caaagtcgaa
    10921 ggcagaaact tcgatatgcg caaacaggtt ttggaatacg acgacgttgc caacgaacag
    10981 cgcaaagtca tttacagcca gcgcaacgaa attctgacca gcaaagacat cggcgacctg
    11041 atgcaggaaa tccgttctga tgccgtcagc gacctcgtgg atacctatat gccgcccgac
    11101 agcatggaag aacaatggga catcccgaca ctggaaaacc gcctggctgc cgaattcagg
    11161 cttcaggaag acatccaatc ctggctgaag gcggacaatg cgattgacgg tcaagacatc
    11221 aaagagcgcc tgatcgaacg tatcgaaaac gaatatgccg ccaaaaccga actggtcggc
    11281 aagcaggcaa tggctgattt cgagcgcaac gtgatgttgc aggccatcga caaccaatgg
    11341 cgcgaacatc ttgccgccat ggactacctg cgacaaggca tacacctgcg cagctatgcc
    11401 cagaaaaatc cgaagcagga atataaacgc gaagccttta ccatgttcca agacctgtgg
    11461 aacggcatca aattccatat cgcctccctg cttacttcgg ttcaaatcga acaaaaccct
    11521 gtcgcggcgg ttgaagagca acccgtcggc aacatccagt ccatccattc cgaatcgccc
    11581 gatatagaag aacttttggg tcagtcgcaa accgatctgg ttaccgaagc ctttaatccc
    11641 gatgggacgg atttcagccc cgaagccttg gaagcgcggg gacaaatcgt ccaccgcaac
    11701 gacccctgcc cctgcggcag cggtttgaaa tacaaacaat gccacggcaa attggcttaa
    11761 gcgtttgaac gcaaatgccg tctgaacatc cggtttccgg cttcagacgg cattttgcct
    11821 gaaccgccac atccgaccgc cattccgaaa aatcccgatt tcgtaccgtc cgtaccgaaa
    11881 acagacatcc cgtcccccca catcatgatt ccatccgact tcattgacga gcttctggcc
    11941 aaaaccgata ttgtcggcat catcgacgag caggttccgc tgaaaaaagg cggggcgaac
    12001 tatatggcgt gttgcccgtt ccacaaggaa aaaacaccgt cgttttcggt cagtccgacc
    12061 aagcagtttt atcattgttt cagttgcggg gcgcatggtt cggcgattgg ttttgtgatg
    12121 gaacatcagg gactgtcgtt tccggaggcg gttcagttcc ttgccgaccg cgtaggtatg
    12181 attgtgccta aagtgcgcgg gcagaacgat aatcccgaag tccgtgccga gcgcaagaaa
    12241 aaacagcaga cgctggaaga aacgacggcg gcggcagctg atttttacgc gcagcagctg
    12301 aaattcaatc cggctgcgaa agcttatttg gacaaacgcg gcttgagcgc ggaagtcatc
    12361 gcgcattatg gtttgggcta cgcgcccgac ggctggcagc ctttggcgca agtgttccaa
    12421 ccgtatccga ataccgcgtt ggtggatacg gggatggtga ttgacaatga ggggcggcat
    12481 tacgaccgct tccgccatcg gattatgttc cccatccgca atccgcgcgg gcaggtcatc
    12541 ggtttcggcg gcagggtgct gggcgactcg aaaccgaaat atttgaattc gcccgatacg
    12601 cctttgttcg ataaggggaa aaacctttac ggcttgtatg aggggcgtgc tgctgtaaaa
    12661 gaggcagaac ggattttggt ggtcgaaggc tatatggacg tggtcgcgct ggcacagttc
    12721 ggcgtgggct acggtgtggc ggccttgggc acggcgacga cggcggaaca cgtcaaaatc
    12781 ctgatgcggc aggcggacag tatttatttc tgtttcgacg gcgacagcgc ggggcgaaaa
    12841 gcggcttggc gcgcgctgga aaacgcgctg ccgcagttga aagacgacaa atcgctgcat
    12901 tttttattcc tgccggaaga acacgacccc gacagctaca tccgcgccta cggcaaagcg
    12961 caatttgaag acgcgcttct gaatcaaagc aagcctttgt cggaatattt ctgggaacac
    13021 ctttcagacg gcattcatct caatacgcag gaaggcaagg cggaattggt gaaaaccagt
    13081 tcgccgcttt tggtgcagat taccgcaccg gcattggctt atttgttaaa acaacggctt
    13141 agcgagctgg tcggcatcga ccccgacaac ctcgcgcaac tgctcggaca ggaagcgcca
    13201 aagcggcacg tcaaacaaaa aaactacaaa ctgcctccga tttccgtcaa acagcccgtt
    13261 atgccgacat tggtgcaacg gcaaatccgc agcctcttga taaatccgga ttgggctgca
    13321 tatatagacc tgcccgatta tctggcgttg gacggcgatt tcgcctgcct tgccaacctc
    13381 gccgaaacca ttaaaaacca tccttccgtg ccggcaaccg cacaggtttt ggaacatatg
    13441 cgcggttcgc cctacgaaga aacaatcaac cgcatcttcc ggtcggccct tcaatcggaa
    13501 gaaatggaag gcggcggcga agaagattgc gaaaacttcc aaatcggcat caaaaaactg
    13561 ctcaatgagt taaaatacag ccaaattgaa acattaaaac aaaaaagcct gcaatccggc
    13621 ttaaatgaaa gcgagaaaaa acttttgctg tcgctgctga ccgcaaaaca aaattgaccg
    13681 gcggattccg ccatctgtaa accgttatgc cgtctgaaaa gcattcaccc cggctgcaac
    13741 aacgacacct gcagaacacc catcccaaaa agccttcaga cggcatcaga gtaccctact
    13801 ttgccacgcc ttcaggtgcg tccaaacgca aaccgtcggc atcttgccaa cagaaagcag
    13861 acaatgtcca aaaaccaaaa tcacgaagaa taccaagacg atgcccgccc cctgacgatt
    13921 gaggaacaac gtgcgcgcct gcgccaactc atcattatgg gcaaagaacg cggctacatc
    13981 acctactccg aaatcaacga cgcactgccc gacgatatgt ccgatgccga tcaaatcgac
    14041 aacatcgtca gtatgatttc cggtttgggt atccaagtta ccgaacacgc ccctgatgcg
    14101 gaagacatat tgttaagcga caatgccgcc gttaccgacg atgatgccgt cgaagaagcc
    14161 gaggccgccc tttccagcgc agattccgag ttcggcagga cgaccgaccc cgtccgtatg
    14221 tatatgcgcg aaatgggaca ggtcgacctg ctgacccgcg aagacgaaat catcatcgcc
    14281 aaaaaaatcg aaaacgccct gaaaaatatg gttcaggcca tctccgcctg cccgggttcc
    14341 attgcggaaa tcttagaact catcgaaaaa atctgcaaag acgaaatccg cgtcgacgaa
    14401 gtcgtagaag ccattatcga cccgaatgaa gtattgctca acgaattggg cttggggcac
    14461 ttggaaacca cagcacccga gaaaccttcc aacgacaatt cggacgaaaa cgaagacgac
    14521 gaagaatcgg aagaagatgc ggatgaagtc tcggcagcca atctcgccga attgaaacaa
    14581 aaagtcatcg gccactttgc ccaaatcgaa aaagactaca aaaaaatgat cggctgtttg
    14641 gaaaaacacc acagccggca caaagactat ctcgcctacc gcgacgcgat tgccaacaaa
    14701 ctgctggaag tccgtttcgc cacccggcaa atcgacagcc tcagcagcag cctgcgcgga
    14761 aaagtagaaa acatccgcaa actcgaacgc gaaatccgcg acatctgcct cgaccgcgtc
    14821 catatggaac gcgactactt catccaaaac ttcctgcccg aaatcaccaa tctgcaatgg
    14881 attgaagaag aaatcgccaa aggcagggtt tggagcaacg cactcgaccg cttccgccac
    14941 gccatcctcg aaaaacaaac cgagttggcg gatatggaaa aagaaacccg catttccatc
    15001 gaagagttga aagaaatcaa caaaaatatg gtgtcgagcg aaaaagaaac cgcagccgcc
    15061 aaacaggaaa tgattcaggc aaacttgcgc ctcgtcattt ccattgccaa aaaatacacc
    15121 aaccgtggct tgcagttcct tgatttgatt caggaaggca acatcggcct gatgaaggcg
    15181 gtcgacaagt tcgaataccg ccggggctac aaattctcca cctacgcaac ctggtggatc
    15241 cgccaggcaa ttacccgctc gattgccgat caggcgcgta ccatccgtat tccggtacat
    15301 atgattgaaa ccatcaacaa gatgaaccgc atctcgcgcc aacaccttca agaaaccggc
    15361 gaagaacccg attccgccaa acttgccgaa ctgatgcaga tgcccgaaga caaaatccgc
    15421 aaaatcatga aaatcgccaa agagccgatt tcgatggaaa cccccatcgg cgacgacgac
    15481 gattcgcact tgggcgactt catcgaagat gccaacaatg ttgcgccggc cgatgcggca
    15541 atgtacacca gcctgcacga agtaaccaaa gaaatcctcg aaagcctgac accgcgtgag
    15601 gcaaaagtcc tgcgtatgcg tttcggcatc gatatgaaca ccgaccacac gctggaagaa
    15661 gtcggcagac agtttgacgt aacgcgcgaa cgcatccgtc aaatcgaggc aaaagcactc
    15721 cgcaagctgc ggcatccgac aagaagcgac cgtttgagaa gtttcttgga cagcgaagac
    15781 agcaagctgt aaaccaaaaa accgcaggtt tcaaatacct gcggtttttt cttacacaat
    15841 aaacaacgct tccacatatc ccacactccc atcccgtttc gccaacaact ccggctgaca
    15901 gatttccacc agagagcagg ctttgcagcg tttgccgtag tcgggcggcg gggtttgtcc
    15961 gctgtttaag agttcgcgga cggcggctat agttgcgagg gtttgggcgc gtaggccgag
    16021 acctttgcaa aaatagtctg ttaacgaaat ttgacgcata aaaatgcgcc aaaaaatttt
    16081 caattgccta aaaccttcct aatattgagc aaaaagtagg aaaaatcaga aaagttttgc
    16141 attttgagaa tgagattgag cataaaattt tagtaaccta tgttattgca aaggtctcag
    16201 gccgtctgaa aacacgacgg ggacacggtg gcgggtttgc atataccaca gcgcgccctc
    16261 tgaaacggtt tgccccgtca tttcttccaa acacaaacct tgggcgcaaa gctggatttc
    16321 atcccccggg tcaggtttgg gcttgcctcg tttgtattcc acaggtttca gacggcctgt
    16381 ttttgtatcc gcttccacca aatccaatat gccgctgatg cccggttttt ccgccaaaac
    16441 atgcactgtc cactcaaagc gcacgccctt gcacgtttcc ggatcgcccg aatctttgcc
    16501 gcaatgatat tgcacgcaat cggcatggga ctttgctccg acacccgata ctgcgcccga
    16561 cgcaacagca cattgccgtt ctgccgcccc tgaagccgtc ccaaataaca cccgttttcg
    16621 gtaaaaaacg ccaaattcac attattttcg ccgcaaaacc ccaacagaaa cggcgacaca
    16681 agcacattcc caaaacagaa aatatgcccg atggaatgca ccggtaactg cgccaccttc
    16741 ttacgctcct gctccaccac cagtgtctcc cgctccttat gcagatagct gccttgggtg
    16801 gtgatgtaga gtgtgttttg cagtttgcgc atgggaaaac tccttaggtt ttgaccgtgc
    16861 ttttggggtt aaacgagggg ctagctaaaa attagatttt atcttctgcc ggatcaactt
    16921 tataccaatg ctcacgattt cttttacact ctaatgctgg tgtagttctt gttacccttc
    16981 ttttcttgct accatcagaa taacgtatat tctcccactc gattggctta ttataatatt
    17041 tcatttctcc accgcactgt ggacattgag aaatactaat tttctctaga cttaaacgat
    17101 gccccatacc gctaatagca taattaaaaa ctaatgggta tctagtttga tacttagtaa
    17161 ttctgcgtaa tgtaatagtg ataagaaata atacagcaat agctattaaa ataaatatat
    17221 aagtattatt ta
//
LOCUS       DAWTHD010000042        17155 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWTHD010000042 DAWTHD010000000
VERSION     DAWTHD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17155
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.042"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            67..804
                     /locus_tag="WM704_001068"
     CDS             67..804
                     /locus_tag="WM704_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFA9340503.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            1053..2456
                     /locus_tag="WM704_001069"
     CDS             1053..2456
                     /locus_tag="WM704_001069"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9340504.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            2743..3120
                     /gene="sdhC"
                     /locus_tag="WM704_001070"
     CDS             2743..3120
                     /gene="sdhC"
                     /locus_tag="WM704_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFA9340505.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            3114..3455
                     /gene="sdhD"
                     /locus_tag="WM704_001071"
     CDS             3114..3455
                     /gene="sdhD"
                     /locus_tag="WM704_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFA9340506.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            3458..5221
                     /gene="sdhA"
                     /locus_tag="WM704_001072"
     CDS             3458..5221
                     /gene="sdhA"
                     /locus_tag="WM704_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFA9340507.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            5341..6048
                     /locus_tag="WM704_001073"
     CDS             5341..6048
                     /locus_tag="WM704_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFA9340508.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            6052..6300
                     /locus_tag="WM704_001074"
     CDS             6052..6300
                     /locus_tag="WM704_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFA9340509.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            6412..7695
                     /gene="gltA"
                     /locus_tag="WM704_001075"
     CDS             6412..7695
                     /gene="gltA"
                     /locus_tag="WM704_001075"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFA9340510.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            7900..10728
                     /locus_tag="WM704_001076"
     CDS             7900..10728
                     /locus_tag="WM704_001076"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFA9340511.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTVKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            10828..12009
                     /gene="odhB"
                     /locus_tag="WM704_001077"
     CDS             10828..12009
                     /gene="odhB"
                     /locus_tag="WM704_001077"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFA9340512.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGFGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            12270..13703
                     /gene="lpdA"
                     /locus_tag="WM704_001078"
     CDS             12270..13703
                     /gene="lpdA"
                     /locus_tag="WM704_001078"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9340513.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            13823..14110
                     /locus_tag="WM704_001079"
     CDS             13823..14110
                     /locus_tag="WM704_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340514.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            14182..15348
                     /gene="sucC"
                     /locus_tag="WM704_001080"
     CDS             14182..15348
                     /gene="sucC"
                     /locus_tag="WM704_001080"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFA9340515.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            15359..16249
                     /gene="sucD"
                     /locus_tag="WM704_001081"
     CDS             15359..16249
                     /gene="sucD"
                     /locus_tag="WM704_001081"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFA9340516.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(16632..17018)
                     /locus_tag="WM704_001082"
                     /pseudo
     CDS             complement(16632..17018)
                     /locus_tag="WM704_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         4373 a         4214 c         4400 g         4168 t
ORIGIN      
        1 ttgcatgggg tagaatgcgc ggcgattcac aattatttct caaaccaatc tattaaggag
       61 ctcaaaatgg ctttgcaaga tcgtaccggt caaaaagtac cttccgtagt attccgcacc
      121 cgcgtcggcg acacttggaa agatgtgtct accgatgatt tgttcaaagg caaaaaagta
      181 gtcgtattct ccctgcccgg tgcatttacc ccgacttgtt cttcttcaca cctgccgcgt
      241 tacaacgaat tattcggcgc gttcaaagaa aacggcgttg acgcaatctg ctgcgtatct
      301 gtaaacgata ctttcgtaat gaacgcttgg gctgccgaag aagaatcaga caacatctac
      361 atgattcctg acggcaacgg cgaatttacc gaaggtatgg gtatgctggt cggtaaagaa
      421 gacttgggct tcggcaaacg ctcttggcgt tactccatgc tggttaacga cggcgtggtt
      481 gaaaaaatgt tcatcgaacc tgaagaaccg ggcgatcctt tcaaagtatc cgatgcagat
      541 actatgctga aattcgttgc tcccgattgg aaggctcaag agtctgtggc aattttcact
      601 aaaccaggtt gccaattctg tgccaaagtc aaacaagctt tgcaagacaa aggtttgtct
      661 tacgaagaaa tcgtattggg caaagatgca accgttactt ccgttcgcgc tattaccggc
      721 aagatgactg cccctcaagt cttcatcggc ggcaaataca tcggcggcag cgaagatttg
      781 gaagcttact tggctaaaaa ctgatagctg tttgcttaag gcagtttgat taaactgtct
      841 gatataccgg atagagttat tcgggcggtt ctatactgcc gctccgaata actctatatt
      901 tacaagaaga tttggatatt gtcgcactca atcgaaattt tgtttttatt tatctgaata
      961 atgtttttga ttgggaagat atttaaatgc tgtctgaaac cgatatgttc tgtgtcggca
     1021 atgtttcaga cgaaaacgga aggacaaaga ttatgaaaaa aattcaagcg gatatcgtcg
     1081 taatcggcgg cggtactgcc ggtatgggtg cgtttcgcaa tgcccgttta cattcggata
     1141 atgtttacct gattgaaaac aatgtgttcg gcacaacctg cgcgcgcgtg ggctgtatgc
     1201 cttccaaact cttgattgcc gccgcagaag cgcgtcacca cgcattgcat accgacccct
     1261 tcggcgtgca tttggacaaa gacagcatcg tcgtcaacgg agaagaggtt atgcggcgcg
     1321 tcaaatccga gcgcgaccgt tttgtcggct ttgtcgttac cgatgtggaa gaatggcctg
     1381 ccgacaagcg catcatgggc tcggctaaat tcatcgacga gcataccgtc caaatcgacg
     1441 accatatcca aattgcggca aaaagtttcg tgattgctac cggttcgcgt cccgtcatcc
     1501 tgccgcagtg gcagtctttg ggcgaccgtt tgattatcaa cgacgacgtt ttctcgtggg
     1561 atacgctgcc taagcgcgtt gccgtgttcg ggccgggcgt tatcggtttg gaactgggtc
     1621 aggcattgca ccgtttgggt gtgaaagttg aaattttcgg tttgggcgga atcatcggcg
     1681 gcatttccga ccccgttgtt tcagacgagg cgaaagccgt gttcggagaa gaattgaaac
     1741 tgcatctgga tgctaaaacc gaggtcaaac tcgacgcaga cggcaatgta gaagtccatt
     1801 gggagcagga tggcgaaaaa ggcgtatttg ttgccgaata tatgttggca gccgtaggtc
     1861 gccgtccgaa cgttgacaat atcggtttgg aaaacatcaa tatcgacaaa gacgcgcgcg
     1921 gcgtacccgt tgccgatccg ctgaccatgc agaccagcat tccgcatatc tttattgcgg
     1981 gcgacgcgtc caaccaactg cccctgctgc atgaagccgc cgaccaaggt aagattgccg
     2041 gcgataacgc gggccgctac ccgaatatcg gcagcggttt gcgccgcagc accattggcg
     2101 tggtgtttac cagtccgcaa atcggctttg tcggtttgaa atacgcgcag gttgccgcgc
     2161 aataccaagc cgacgaattt gtcatcggcg aagtatcgtt caaaaatcaa ggtcgcagcc
     2221 gcgtgatgct ggtaaacaaa ggccatatgc gcctgtatgc cgaaaaagcc accggccgct
     2281 ttatcggcgc ggaaatcgta ggccctgccg ccgaacattt ggctcacttg ttggcatggg
     2341 cgcatcaaat gaagatgacc gttccgcaaa tgctggatat gccgttctac catcctgtta
     2401 tcgaggaagg tctgcgtacc gcgttgcgcg atgccgatgc gaaattgaaa gcctgaccga
     2461 tatggcaaaa caatgccgcc tgaaattttt ttcagacggc attttatttt tggggatggg
     2521 gttgggggtc ggatgctgat accgtgtcgg gaagggagcg gcaaaactaa aaatctttct
     2581 tttaatctgc tgtttccacg cgtgtttgtc aaaatctatc agtttgtttt taaaatacac
     2641 tgttcaaaat gggataaaac aggtaaatta acgttgatgt aacccagtgt agcaatgggt
     2701 ttacggtttt tgagtcgata tataactaca gaggaattga ctatgtctgt caaactgcgt
     2761 cctgtttatc tggatttgcc gaacatccgt ctgccgatac ccgggatagt ttccatcctt
     2821 caccgcatta gcggggtcgg gctgtttatt atgctgcctt tcctgctgta ttttctgtcc
     2881 ggtaccctga gtcaagagtc tgcatttgaa acttaccgtg ccattgtttc ccatcctttg
     2941 gtcaagctgg ttttaatcgg tatattgtgg gcttatctgc accattctct cgccggtatc
     3001 cgctttttat ttttggatgc gcacaaaggc cttgagctga atactgcgcg caataccgct
     3061 aaagccgtat ttgcttccgc attggttttg actgtcgttt tgggagcgtt gttatggtag
     3121 aacgtaaatt gaccggtgcc cattacggtt tgcgcgattg ggtaatgcag cgtgcgactg
     3181 cggttattat gttgatttat accgttgcac ttttagtggt tctatttgcc ctgcctaaag
     3241 aatatccggc atggcaggca ttttttagtc aagcttgggt aaaagtattt acccaagtga
     3301 gctttatcgc cgtattcttg cacgcttggg tgggtatccg cgatttgtgg atggactata
     3361 tcaaaccctt cggcgtgcgt ttgtttttgc aggttgccac cattgtctgg ctggtcggct
     3421 gcctcgtgta ttcagttaaa gtgatttggg ggtaagtatg ggttttcctg ttcgcaagtt
     3481 tgatgccgtg attgtcggcg gtggcggtgc aggtttacgt gcagccctcc aattatccaa
     3541 atccggtttg aattgtgccg ttttgtctaa agtgttcccg acccgctcgc ataccgtagc
     3601 ggcgcagggc ggtatttccg cctctctggg taatgtgcag gaggaccgtt gggactggca
     3661 catgtacgat accgtgaaag gttccgactg gctgggcgac caagatgcga ttgagtttat
     3721 gtgtcgcgct gcgcctgaag cggtgattga gttggaacac atgggtatgc cttttgaccg
     3781 cgttgaaagc ggcaaaattt atcagcgtcc tttcggcgga catactgccg aacatggtaa
     3841 acgtgcggta gaacgtgcat gtgcggttgc cgaccgtacc ggtcatgcga tgttgcatac
     3901 tttgtaccaa caaaacgtcc gtgccaatac acaattcttt gtggaatgga cggcgcaaga
     3961 tttgattcgt gatgaaaacg gcgatgtcgt cggcgtaacc gccatggaaa tggaaacggg
     4021 cgaagtttat attttccacg ccaaggccgt gatgtttgct accggtggcg gcggtcgtat
     4081 ttatgcttct tctaccaatg cttatatgaa taccggtgac ggtttgggca tttgcgcccg
     4141 tgcgggcatt ccgttggaag atatggaatt ctggcaattc cacccgaccg gcgtggcggg
     4201 tgcgggcgtg ttgattaccg aaggcgtacg cggcgagggc ggtattctgt tgaacgccga
     4261 cggcgaacgc tttatggaac gctatgcgcc gaccgtaaaa gacttggctt ctcgcgacgt
     4321 ggtttcacgc gcgatggcga tggaaatcta tgaaggtcgc ggctgtggta aaaacaaaga
     4381 ccacgtctta ctgaaaatcg accatatcgg tgcagaaaaa attatggaaa aactgccggg
     4441 catccgcgag atttccattc agtttgccgg tatcgatccg attaaagacc cgattccggt
     4501 tgtgccgact acccactata tgatgggcgg cattccgacc aattatcacg gtgaagttgt
     4561 tgttccgcaa ggcgacgagt acgaagtacc tgtaaaaggc ctgtatgccg caggtgagtg
     4621 cgcctgtgct tccgtacacg gtgcgaaccg tttgggtacg aactccctgc tggacttggt
     4681 ggtgttcggc aaagctgccg gtgacagcat gattaaattc atcaaagagc aaagcgattg
     4741 gaaacctctg cctgctaatg ccggtgagtt gacccgccaa cgtatcgagc gtttggacag
     4801 tcaaaccgat ggtgaaaacg ttgatgcatt gcgtcgcgaa ctgcaacgct ccgtacaact
     4861 gcacgccggc gtgttccgta ccgatgagat tctgagcaaa ggcgttcaag aaatcatggc
     4921 gattgccgag cgtgtgaaac gtaccgaaat caaagacaag agcaaagtgt ggaataccgc
     4981 gcgtatcgaa gctttggaat tggataacct gattgaagtg gcgaaagcga ctttggtgtc
     5041 tgccgaagca cgtaaagaat cacgcggtgc gcacgcttca gacgaccatc ctgagcgcga
     5101 tgacgaaaat tggatgaaac acaccctgta tcattcggac accaatacct tgtcttacaa
     5161 accggtacac accaagcctt tgagcgtgga atacatcaaa ccggccaaac gcgtttattg
     5221 atgcgttttc agacggtctt cgcctcaaag gtcgtctgaa acctaaccat acccacattg
     5281 aactgcttga atttataata caaaatcatt gggcagttga tgagaaaagg aacacttctc
     5341 atggaaaaaa tgagttttga aatttaccgt tacaacccgg acgttgatgc caagccttat
     5401 atgcagcgtt acgagttgga attggaaccg accgacgtga aacttttgga cgctttggta
     5461 cgcctgaaag cacaagacga taccttgtct ttccgccgct cctgccgcga agggatttgc
     5521 ggatcggacg gtatgaacat caacggcaaa aacggcttgg cgtgtctgac cgatttacgc
     5581 agcttgaaac agcctgtcaa aatccgcccg ctgcccggtc tgcccgtcat ccgcgacctg
     5641 attgtggata tgacccagtt cttcaaacaa taccattccg tcaaacctta tgttgttaac
     5701 gacaatccga ttgatgcgga caaagagcgt ctgcaaactc aggaagagcg caaagagttg
     5761 gatggtttgt acgaatgtat tttgtgcgcc tgctgttcga ccgcctgtcc gtcattctgg
     5821 tggaatcccg acaaattcgt cggtccgtcc ggcttgctga acgcctaccg cttcattgcg
     5881 gacagccgag ataccatcac taatgagcgt ttggataatt tgaacgatcc gtaccgtttg
     5941 ttccgctgcc acaccattat gaactgcgtg gacgtatgtc ctaaacactt gaatccgacc
     6001 cgggccatcg gtaagattaa agagattatg ttgaaacggg ttgtttaaga aatgatggtt
     6061 tttgacgaca ttgccaaacg gaaaatccgt tttcaaaccc gccggggatt gttggaacta
     6121 gatttaatct tcggcaggtt tatggaaaaa gaattcgagc atttgagcga taaagagctg
     6181 tccgagtttt ccgaaatcct tgaatttcaa gatcaagaat tgcttgcctt gattcacggg
     6241 cattcggaaa cggacaaagg gcaccttatc ccgatgcttg aaaaaatcag acgggcatga
     6301 ggcatctgaa atttgtctga gggcagattt caaaatgcaa aggccgtctg aaagcaaaga
     6361 acgtgctgcg gatgcagtaa cgtgggttat aacttgcaaa ggagcgataa tatgtccaaa
     6421 tcaatcaaac tcaacgtacc gggtcgggca ggtttggagc tgccggtatt ggaagccagc
     6481 atcgggcatg atgtggttga cattcggggg ctgacaaaaa atacaggttt gtttgccttc
     6541 gaccccggat ttgtttcaac cgcaagctgt gagtctaaaa ttacttacat cgacggcgat
     6601 caaggcttgc tttattatcg cggatacccc atcgagcagc tggccgaaaa gtccgattat
     6661 ttggaagtct gctacctgtt gatttacggc gaactgccga ctcccgagca aaaggcagaa
     6721 tttgacaata cagtccgccg ccacacgatg gtgcatgaac agctgacttg gttcttccgg
     6781 ggtttccgcc gcgacgcgca tccgatggcg atgatggtcg gcgtggtcgg cgcgctgtct
     6841 gcgttctacc aagacagctt ggacattact aatcccgaac accgcaaaat cgcgatttac
     6901 cgccttattt ccaaaatccc gaccattgcg gcaatgtgtt accgctattc aaacggtctg
     6961 ccgttcaatt atccgaagaa taatctttct tattcagaaa acttccttca tatgatgttt
     7021 gccacaccgt gtgaagacta caaacctaat cccgttttgg cacgcgcgct cgaccgcatc
     7081 tttattttgc atgccgacca cgagcaaaac gcctcaactt caaccgtccg tctggcaggc
     7141 tcttcgggtg cgaacccgtt tgcctgtatt gctgccggta tcgcctgcct gtggggtgct
     7201 tcgcacggcg gtgcgaacga agccgtgttg aaaatgctgg atgaaatcgg cgatgtgtcc
     7261 aatgttgccg catacatgga aggcgtgaaa cagcgcaaat accgcctgat gggcttcgga
     7321 caccgggtat accgcaatat ggatccgcgt gccagcatta tgcgcgaaac ctgctatgaa
     7381 gttttgaagg aattgggctt ggaagacagt ccgaaattca aactggcgat ggaattggaa
     7441 cagattgcgc tgaaagaccc gttctttatc gaacgcaaac tgtatcccaa tgtcgatttc
     7501 tattccggca tcgtcctgtc cgcgctgggc atcccgaccg aaatgtttac cgtcatcttc
     7561 gccctgtcgc gcagcgtggg ctggatttcg cactggcacg agatgattag cgatccttcg
     7621 ctgaaaatcg gccgcccgcg ccagctttat accggttcgg aacgccgcga ttatgtgccg
     7681 gcaggcgaga ggtaacatac attaatatat tgtcaaacag gcaatatcag agaaccggat
     7741 tgtttcctga atccgtctga ttgtagtcgg atgaaatcaa gacaagcaat ccggtttaaa
     7801 atagggtaga ataaaatgtc ttttcagacg gcatcagttc agccgtcagg acgcggactt
     7861 ctaccctttg tttatatttt aaagaaaaga gcgcacgcca tgatggacga aaaactcaat
     7921 ttctcttatc tgttcggttc gaacgcaccc tacattgagg aattgtacga ggcttttttg
     7981 gaaaaccccg atgcggttga tgaaaaatgg aagcagtatt tcaccgattt gagcaaacag
     8041 ccggggacgg ttgctgtcga tgtcgcacac acaccgattc gcgaatcatt tgttactttg
     8101 gcgaaaaaga aaattgcatc tgccgttgcg ggcggtgcgg atgaggcaat gctgaaaaag
     8161 caagtcagcg ttttacggct gatttctgcc tatcgtatcc aaggcgtggg tgcggcccaa
     8221 cttgatccgc tcaaacgtat ccctccgcgc gatattgaag ccctcgatcc gaaattccac
     8281 ggtctgtcag atgccgatat ggcgcttcga ttcaatatgg gcgagggtga ttttgccaat
     8341 cgcggcaaac tgcccttgtc ccaaatcatc agcaacctca aacaaaccta ctgcggccac
     8401 atcgcattgg aatatatcta tattcccaat accgaagagc gccgttgggt acgcaactat
     8461 tttgaaagcg tattgtccac accgcattac aatgccgatc aaaaacgccg tatcttgaaa
     8521 gagatgaccg ctgccgagac tttggaacgt tatctgcata ccaaatatgt cggtcagaaa
     8581 cgtttcggtg tcgaaggcgg cgaaagcgcg attgccggtt tgaactacct gattcaaaac
     8641 gccggtaagg acggcgtgga agaagtcatc atcggtatgg cgcaccgtgg ccgtctgaat
     8701 gttttggtga acattttggg caaaaaaccc ggcgatttgt ttgccgaatt tgaaggccgt
     8761 gccgaaatca aactgcctag tggcgacgtg aaataccata tgggcttcag ctccgatatt
     8821 gccacccctc acggcccgat gcacgtttct ttggcgttca acccgtcaca cttggaaatc
     8881 gtcaatccgg tagtggaagg ttctgcacgc gccaaacaaa aacgtttggg cgaaaacggc
     8941 cgcgataaag tcttgccggt attgattcac ggcgattccg catttatcgg tttgggtgtc
     9001 aaccaagcaa cattcaacct gtctaaaacg cgcggttata ccacaggcgg tacggtccat
     9061 atcgtcatca acaaccaaat cggctttacc acttccgata tccgcgatac ccgttcaacc
     9121 gtacactgta ccgatatcgc aaaaatggtt tccgctccgg ttatccatgt gaacggcgat
     9181 gatcccgaac gcgtttgctt tgccattcaa gccgctttgg attaccgcaa aaaattccat
     9241 aaagacatcg taatcgacgt tgtctgctac cgtaaatggg gtcacaacga gggcgatgat
     9301 ccgactttga cccaaccgat gatgtacaaa aaagtatcgc aacatccggg tgcgcgtgct
     9361 ttgtacaccg agcaactgat tgccgaaggc gtggtaactc aagttgaggc cgacggttac
     9421 atccaagcct accgtgatgc tttggacaaa ggcgaacatg ttgagcaaac aacgttgagc
     9481 aacttccaac gcacacaaat cgactggagc aaataccaag gtaaagattg gcgcgaaaaa
     9541 atcgaaaccg gtttgcctgc cgccgacatt gagcgtctta ctgagaaatt caccgctgtg
     9601 ccggaaggct ttgccctgca tccgactgta aaacgtgtga tcgaagcgcg taaagccatg
     9661 gcatccggca aacaagccat cgactggggt atggccgaaa ccctcgcata cgcaagcctg
     9721 ctgaccaaag gtcatggcgt gcgtatctcc ggtgaggact ccggccgcgg tacattctca
     9781 caccgtcatg ccgttctgca cgatcaaaaa cgcgaaaaat gggacgacgg tacttatgtt
     9841 cctcttcgca acatgggcga aggcttgggc gagttcctgg ttatcgactc tatcttgaac
     9901 gaagaagccg tgatggcgtt cgagtacggc tttgcctgct ccgctcctga caagctgacc
     9961 atttgggaag ctcaattcgg tgacttcgcc aacggcgcgc aagtaactat tgatcaattc
    10021 ctgtcttcag gcgaaaccaa gtggggtcgc ttgtgcggtc tgaccaccat cctgccgcac
    10081 ggctacgacg gtcaaggccc tgaacactct tctgcacgcg tagaacgttg gttgcaactg
    10141 tgttctgaga acaatatgca agtcatcatg ccgtctgaag cgtcgcaaat gttccatctc
    10201 ttgcaacgcc aagtcttggg ttcataccgc aaaccgctgg tgattttcat gtccaaacgc
    10261 ctgttgcgct tcaaaggtgc aatgagcccg ctggaaaact tcaccgaagg ttcgactttc
    10321 cgtccggtta ttggtgatac cgccgaacgc gcaagcaacg acagcgtgaa acgcgtggta
    10381 ttgtgtgccg gtcaggttta ctatgacttg gaagccggtc gagccgaacg taaactggaa
    10441 gatgatgtcg ctatcgtccg cgttgagcag ctgtatccgt tcccatacga cgaggttaaa
    10501 gccgaactgg cgaaatatcc gaacgcaaaa tctgtggttt gggcgcaaga agagccgaaa
    10561 aaccaaggcg cgttctacca aatccgccac cgcatcgaag acgttatcag cgaagagcaa
    10621 aaactgtctt atgccggccg tccaagcagc gcatcgcctg cagtgggcta ctcaagcaaa
    10681 cacattgctc aattgaaaca attggttgaa gacgctttgg cgttataaac caagtagcat
    10741 tccgtctgag tctgctcagg cggaatgccc atatgcagaa ttaaaaacac acaacaggcc
    10801 gtctgaaagg gccattggag acacaaaatg attattgatg taaaagtacc tatgctgtct
    10861 gaaagcgtat ctgaaggcac actcttggaa tggaagaaaa aagttggcga agcagttgcc
    10921 cgtgacgaaa tcctgatcga tatcgaaacg gacaaagtgg ttttggaagt accttctcca
    10981 caagccggcg tattggttga aatcgtagcg caagacggtg aaaccgttgt tgccgaccaa
    11041 gttttggccc gtatcgatac agctgctact gtcgctgctg aagcacctgc agccgctcct
    11101 gccgaagctg ccccggctgc cgttcctgct gctgcacaaa acaacgccgc catgcctgcc
    11161 gctgcaaaac tggctgccga gaccggtgtt gacgtgaacg tattgcaagg tttcggccgt
    11221 gacggtcgcg tattgaaaga agacgtacaa aatgccgctg ccaaacctgc cgcagccgtg
    11281 gcccctgccg ttgcacttcc tgccggcgca cgtcctgaag aacgcgtacc aatgagccgc
    11341 ctgcgtgccc gtgttgcaga acgcctcttg gcttctcaac aagaaaacgc cattctgact
    11401 acattcaacg aagtcaacat gaaaccgatc atggacttgc gtgcgaagta caaagaaaaa
    11461 ttcgagaaag aacatggcgt aaaactgggc tttatgtcct tcttcgttaa agccgctgtt
    11521 acagccctga aaaaataccc ggttgtgaat gcttctgttg acggcaaaga cattgtgtac
    11581 cacggctact tcgacatcgg tatcgcaatt ggcagcccac gcggtttggt tgtaccaatc
    11641 ctgcgcgatg ccgaccaaat gagcattgcc gacatcgaac aagcaattgt tgattacgcg
    11701 aaaaaagcca aagacggcaa aatcgctatc gaagacctga ccggcggtac attcagtatt
    11761 accaacggcg gtactttcgg ttctatgatg tccaccccga tcatcaaccc gcctcaatct
    11821 gcgattttgg gtatgcatgc cactaaagag cgcgctgtgg ttgaaaacgg ccaagttgtt
    11881 gtccgtccaa tgatgtatct ggctctgtct tacgaccacc gtatcattga cggccgcgaa
    11941 gctgtattga ccttggtagc cattaaagac gcgttggaag acccggtccg cctgttgttg
    12001 gatctgtaat cgtttcagac ggctttttat ttgttaatga aaaggccgtc tgaattttta
    12061 tttagatgta gcgtaatgta gtatcgtgct acaataggct caacgaacga ttgaggccgt
    12121 ctgaaacatt tgattcgaat gaatcggcag atatggactt tcagacggct ttttcttaaa
    12181 accatcaaaa cgcagtcatt caaaataaaa aaagaaacga aaagtatcgt ttttattttg
    12241 agatactgct aaaagcaaag gatgacacga tgtctcaata tgatgtagta gtgattggtg
    12301 caggtccggg cggatacgtt gccgccatcc gtgccgcaca actgggtttc aaaactgcct
    12361 gtgtcgatgc aggcgttaac aaagcaggca atgcccctgc attgggcggt acttgcttga
    12421 acgtaggctg tatcccttct aaagccctgt tgcaatccag cgaacatttc cacgctgcgc
    12481 aacacgattt tgccgaacac ggtatcactg tcggcgacgt aaaatttgac gtggccaaaa
    12541 tgattgagcg caaagatgcc atcgtgacca aactgaccgg cggcgtgaaa ttcctgttcc
    12601 aaaaaaacaa agtaaccagc ctgttcggta ctgcttcctt tgccggtaaa aatggcgatg
    12661 cttaccaaat tgaagtcgat aacaaaggcg agaaaaccgt tatcgaagcc aaacacgtca
    12721 tcgtagcaac cggttccgta ccgcgtccgt tgccgcaagt cgctatcgac aacgtgaacg
    12781 tattggacaa cgaaggcgca ttgaacctga ccgaagtacc tgccaaactc ggcgtaatcg
    12841 gttccggtgt gattggtttg gaaatgggtt ccgtatggaa ccgcgtgggt gcggaagtta
    12901 ccattcttga agccgcgccg accttcctgg ctgccgccga ccaacaaatc gccaaagaag
    12961 ccttcaaata cttcaccaaa gagcaaggtc tgagcatcga attgggtgtg aaaatcggcg
    13021 acatcaagtc tgaaggcaaa ggtgtttccg ttgcttacga aactgctgcc ggcgaagcca
    13081 aaaccgaagt attcgacaaa ctgatcgttg ccatcggccg tattccaaac accaaaggcc
    13141 tgaacgctga agccgtaggc ttggaaaaag acgagcgcgg ctttatcaaa gtggatggcg
    13201 aatgccgtac caacctgcct aacgtatggg caatcggcga cgtggttcgc ggcccgatgt
    13261 tggcacacaa agccagcgac gaaggcgttg ccgttgccga gcgcattgcc ggtcaaaaac
    13321 cgcatatcga cttcaacaac gtaccgtttg tgatttacac cgaccctgaa atcgcttggg
    13381 tgggtaaaac cgaagagcag ctcaaagccg aaggcgtgga gtacaaaaaa ggtacttcag
    13441 gtttcggtgc aaacggccgt gcattggcaa tgggcaaagc caaaggtacg gttaaagtgt
    13501 tggcagatgc caaaaccgac cgtatcttgg gcgtacacat gatcggcccg gttgtcagcg
    13561 aattggttac cgaaggcgtg actgcgctcg aattcttcgc cagcagcgaa gatatcgccc
    13621 gtattatcca tgcccaccca accttgtccg aagtggttca cgaagctgca ttggcggccg
    13681 acaaacgcgc tttgcacggt tgatagacat taaggccgtc tgaaattttc agacggcctt
    13741 aaggcctccg gcaaattgaa tgttccgaga gcttcgtttt ctgatttata attccgtcag
    13801 acaaacagca tttacattca ttatgaacaa agaaatagtc ggtattttct ttataccgat
    13861 gggcatcatc agcatgtgta tggctgcatt gtggcagatg tatgtgatga tgaccgaaac
    13921 ttatacgctc aaccgtttca aagataaaga attggtttgg cgcgtggcat tgttgtttat
    13981 cagtttcagc cttgccgttt atctgctctg tccgaattcg cgtaaaaaag gcatcgtctt
    14041 ttttattctc gggggaggcg gtgcagtcat gtatctgctg gcgcggatgt ggttgccctt
    14101 cagtaaatag taggaccgtc tgaaaatatg ggattggccg cagggcgatt cctaaaaccc
    14161 actcacctta aggagaaatc catgaattta cacgagtatc aggctaaaga actgctggct
    14221 agctacggtt tgcccgtaca aggcggtatt ttggcgcaca acggcgaaga agccgctgca
    14281 gcttacgaca aattgggcgg caaattcgct gttgtcaaag cacaggtaca cgccggcggc
    14341 cgcggtaaag cgggcggcgt aaaagtcgtt aaaagccgcg aaaaagccaa agaagtggct
    14401 gaaagcctga ttggcaccaa cttggtaact taccaaaccg atgccaacgg ccaacctgtc
    14461 aacagcgttt tggtttgcga agacatgtac cctgttcaaa ccgagctgta cttgggcgca
    14521 gtggttgacc gttctacccg ccgcgttaca ttcatggctt ctaccgaagg cggcgtggaa
    14581 atcgaaaaag ttgctgctga aactccagaa aaaatcttca aagtaaccgt tgatccgctg
    14641 gtcggcctgc aaccttgcca agctcgcgaa gttgccttcc aactgggctt gaaagacaaa
    14701 caaatcaacg agttcgccaa actgatgacc ggcgcgtaca aagcgtttgt cgaaaacgac
    14761 ttcgccctgt ttgaagtcaa cccgctggca gttcgcgaaa acggcgcact ggcttgcgtg
    14821 gacggcaaaa tcggcatcga cagcaacgcg ctctaccgcc tgccaaaaat cgctgaattg
    14881 cgcgacaaat ctcaagaaaa cgaacgtgaa ctgaaagctt ctgaattcga cctgaactat
    14941 gttgccctgg aaggtaatat cggctgtatg gttaacggtg ccggtttggc gatggccacc
    15001 atggacatca tcaaactcaa aggcggccaa cctgccaact tcttggacgt tggcggcggc
    15061 gcaaccaaag accgcgtggt tgaagcgttc aaactgattt tggaagacaa atccgttaaa
    15121 ggcgtattga tcaacatctt cggcggtatc gtacgttgcg acatgattgc ggaagccatc
    15181 gtggcagccg ttaaagaaat caacgtcaac gttcctgtcg ttgttcgttt ggaaggcaac
    15241 aacgccgaac tcggcgcgaa aatcctgaac gaatcaggtc tgaaactgac ttctgcagac
    15301 ggtctgaatg acgcagccga aaaaattgtt gcagccgtaa acgcctaagg agaaaagaat
    15361 gagcgtattg attaataaag acaccaaagt attggttcaa ggtttcaccg gtaaaaacgg
    15421 tactttccac tccgaacaag ctctggctta cggcactaaa gttgtcggcg gcgttacccc
    15481 aggcaaaggc ggtcaaaccc acctagacct gcctgtattc aacaccatga aagaagccgt
    15541 taaagaaacc ggcgctgacg catccgtgat ttacgttccc gctccgtttg tgttggattc
    15601 tatcgttgaa gccgttgatt caggcgtagg cttggtcgtt gtgattaccg aaggcgttcc
    15661 aactctggac atgctcaaag ccaaacgtta cttggaaacc aacggcaacg gtacccgttt
    15721 ggtcggccct aactgcccgg gcgtgattac tccgggcgag tgcaaaatcg gcattatgcc
    15781 gggccacatc cacactccgg gccgcatcgg tattatttcc cgttccggta cattgactta
    15841 cgaagccgtg gcacaaacca ccaaactggg cttgggtcaa tcaacctgta tcggtatcgg
    15901 cggcgacccg attcctggta tgaaccaaat cgacgcactg aaactcttcc aagaagaccc
    15961 agataccgat gccatcatca tgattggtga aatcggtggt actgcggaag aagaagcagc
    16021 cgaatacatc caatccaacg taaccaaacc tgttgtcggt tacatcgccg gtgttaccgc
    16081 tcctaaaggc aaacgcatgg gccacgccgg tgcgattatc tccggcggta aaggtactgc
    16141 cgaagaaaaa ttcgccgcgt ttgaaaaagc cggtatcgcc tacacccgca gccctgccga
    16201 gttgggcacg acgatgctgg aagtgttgaa agcaaaaggt ttggcataat caggtttgac
    16261 aactgattga acatcaaatg ccgtctgaaa ccggaaatcg ggtttcagac ggcattttgt
    16321 ttgtcatttc aaaaagaggc agcctcaaca tactcacatt atttttgccc tttgaggcag
    16381 tcagaatata gtggattaaa aacaaaatag tacaacactc aacgttgaag gtctaaccat
    16441 ggcatactct gcggacttaa gaaacaaagc tttaaaccat agcggattaa caaaaatcag
    16501 gacaaggcgg cgggccgcag acggtacaaa tggtacggaa ccgatccgcc cggtgcttca
    16561 tcaccttagg gaatcgttcc ctttgagccg gggcggggca acgccgtacc ggtttttgtt
    16621 aatccgctat acagcaggcg ttggtggaaa tcccgaataa gcggttcaaa acgtgtcgct
    16681 gccgcggcag ccatatacag cgccttacgc accgcagacc ttccgccgaa gcagcggctt
    16741 ttgaatttgg tttccccgct ctccctcggg tgcggggcaa tgccgaccaa acccgctatc
    16801 cgtttgtgcg acagccgccc caattcgggc agcatcgcca tcagcgtagc cgtcgttatc
    16861 gaaccgatgc ctttgatttg ctccgccact tgggctttgc cgtcaaaatg cgtgcgggtg
    16921 tggttgtcga tttgtttgtc cgattcgtca atcagccggt caaaacgggc aatcagttgt
    16981 ttgacgcttt cgacttgcgt ttcgtgaacc tgatgcagac attttgtaat agctgccatt
    17041 acgcctacta ggatatatac cttctatata tagtgaatta acaaaaaccg gtacggcgtt
    17101 gccccgcccc ggctcaaagg gaacgattcc ctaaggtgat ggagcgccgg gcgga
//
LOCUS       DAWTHD010000043        17061 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWTHD010000043 DAWTHD010000000
VERSION     DAWTHD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17061
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.043"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(52..390)
                     /locus_tag="WM704_001083"
     CDS             complement(52..390)
                     /locus_tag="WM704_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFA9340517.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            542..4498
                     /gene="purL"
                     /locus_tag="WM704_001084"
     CDS             542..4498
                     /gene="purL"
                     /locus_tag="WM704_001084"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFA9340518.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            complement(4569..5780)
                     /locus_tag="WM704_001085"
     CDS             complement(4569..5780)
                     /locus_tag="WM704_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFA9340519.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5854..6165)
                     /locus_tag="WM704_001086"
     CDS             complement(5854..6165)
                     /locus_tag="WM704_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9340520.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            6397..7149
                     /gene="gloB"
                     /locus_tag="WM704_001087"
     CDS             6397..7149
                     /gene="gloB"
                     /locus_tag="WM704_001087"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFA9340521.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDNFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAEHTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            7383..8837
                     /gene="mgtE"
                     /locus_tag="WM704_001088"
     CDS             7383..8837
                     /gene="mgtE"
                     /locus_tag="WM704_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFA9340522.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(8923..9831)
                     /gene="hslO"
                     /locus_tag="WM704_001089"
     CDS             complement(8923..9831)
                     /gene="hslO"
                     /locus_tag="WM704_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFA9340523.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AISCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSANDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            10076..10657
                     /locus_tag="WM704_001090"
     CDS             10076..10657
                     /locus_tag="WM704_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9340524.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGSAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGAPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            10676..10894
                     /locus_tag="WM704_001091"
     CDS             10676..10894
                     /locus_tag="WM704_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340525.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            11039..11383
                     /locus_tag="WM704_001092"
     CDS             11039..11383
                     /locus_tag="WM704_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFA9340526.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            11383..12075
                     /locus_tag="WM704_001093"
     CDS             11383..12075
                     /locus_tag="WM704_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFA9340527.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            12142..13362
                     /gene="argJ"
                     /locus_tag="WM704_001094"
     CDS             12142..13362
                     /gene="argJ"
                     /locus_tag="WM704_001094"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFA9340528.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            13422..14831
                     /locus_tag="WM704_001095"
     CDS             13422..14831
                     /locus_tag="WM704_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFA9340529.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            15019..16410
                     /gene="hrpA"
                     /locus_tag="WM704_001096"
     CDS             15019..16410
                     /gene="hrpA"
                     /locus_tag="WM704_001096"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFA9340530.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDDTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEEN"
     gene            complement(16772..>17061)
                     /locus_tag="WM704_001097"
     CDS             complement(16772..>17061)
                     /locus_tag="WM704_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340531.1"
                     /translation="EQFYMILKKKIYDDYDKSLEAAAWQTILNQSIGISELLRFFDKL
                     SSKDIENMGRFWLEFCCNFPKYTQSLPFLIRQALLNHGFSVNDIIEYQASW"
BASE COUNT         3881 a         5104 c         4379 g         3697 t
ORIGIN      
        1 aaaacaaatg ccgtctgaaa gcctttcaga cggcatttcc attccaaacg ctcaaaccgc
       61 cgcgtccgaa cgttcgcccg tgcggatacg gattgcctcc tcgaccggca gcacaaaaat
      121 cttgccgtcg ccgattttgc ccgaacgcgc cacctcgaca atcacgtcaa tcgcgcgttc
      181 cacggcatca tccgccaaca ccaactcgat tttgaccttg ggcaggaaat cgacggcgta
      241 ttccgcaccg cgatagattt ccgtatgccc cttctgcctg ccgaaccctt tgacctcgct
      301 gacggtcatg cccgtaatgc cgatttccgt caacgcctcg cgcacgtcgt cgagcttgaa
      361 cggtttgaca atcgcctcga tttttttcat aaaatttcct ttgaacaaac aatacaaaca
      421 catccgaaaa acgggaaccc cccgtcagat tgtcaacatt ttaaaccaaa atacccaagc
      481 aatacagccc ccgttgcgca tataatgaca gcttttccaa ccgcatttga gagccgaatc
      541 catgtctgtt gttttgccct tgcgcggcgt taccgccctt tccgatttcc gtgtcgaaaa
      601 actcttgcaa aaagccgccg cactcggcct gcccgaagtc aaattaagca gcgaattttg
      661 gtatttcgcc ggcagcgaga aagcacttga tgccgcgact gtcgaaaaac tgcaagcctt
      721 gttggcggcg caaagcgttg aacaaacgcc cgaagcgcgc gagggtttgc atttgttttt
      781 ggtaacgccc cgtttgggta cgatttcgcc gtgggcttcc aaggcgacca atatcgcgga
      841 aaactgcggt ttggcaggca tcgaacgcat cgagcgcggt atggcggtgt ggctggaagg
      901 tgcgcttacc gatgaacaga aacagcaatg ggcggcgttg ctgcacgacc gcatgaccga
      961 atccgtgctg cccgattttc agacggcatc caaattattc caccatctca aatccgaaac
     1021 cttctccacc gtcgatgttt tgggcggcgg taaagaggct ttggtcaaag ccaataccga
     1081 aacgggtttg gcactctccg ccgacgaaat cgattatctg gttgaaaact atcaggcttt
     1141 gcagcgcaat ccgtccgatg tggagctgat gatgttcgcg caggcaaaca gcgaacactg
     1201 ccgccacaaa atcttcaacg ccgatttcat cctcaacggc gaaaaacaac cgaaatccct
     1261 gttcggcatg atacgcgaca cgcacaacgc gcatcccgaa ggtacggtcg tcgcctataa
     1321 agacaattcg tccgtgatca aaggcgcgaa aatcgagcgt ttctatccga atgcagcgga
     1381 aaaccaaggc taccgtttcc acgaggaaga cacccacatc atcatgaaag tggaaacgca
     1441 caaccacccg accgccatcg cgccgtttgc gggcgcggca acaggcgcgg gcggcgaaat
     1501 ccgcgacgaa ggtgcgacgg gcaaaggttc gcgtccgaaa gcgggcttga ccggctttac
     1561 cgtgtccaac ctcaacatcc ccgacctcaa acagccgtgg gaacaagact acggcaagcc
     1621 ggaacacatt tcctcgccgt tggacatcat gattgaaggc ccgattggcg gtgcggcgtt
     1681 caacaacgaa ttcggccgcc cgaacctctt gggctacttc cgcacctttg aagaaaaatt
     1741 tgacggtcag gttcgcggct accacaaacc gattatgatt gccggcggtt tgggcagcat
     1801 tcaggcgcag cagacgcaca aagacgaaat ccccgaaggc gcactgctga tccaactggg
     1861 cggcccgggc atgctcatcg gcttgggcgg cggtgcggct tcttcgatgg ataccggtac
     1921 aaatgatgcg tctttggact tcaactccgt acaacgcggc aaccctgaaa tcgaacgccg
     1981 cgcgcaggaa gtcatcgacc gctgctggca gctcggcgac caaaacccga ttatctccat
     2041 ccacgacgtg ggcgcgggcg gcttgtccaa cgctttccct gaattggtca acgatgccgg
     2101 acgcggcgcg gtattcgagc tgcgcgaagt gccgttggaa gaacacggtt taacgccttt
     2161 gcaaatctgg tgtaacgaat cgcaagagcg ttatgtcttg tcgattttgg aaaaagattt
     2221 ggacaccttc cgcgccatct gcgaacgcga acgctgcccg tttgccgtag tcggcacggc
     2281 gaccgacgac ggtcatttga aagtgcgcga cgatttgttc tccaacaacc ccgtcgattt
     2341 gccgctgaac gtcttgctcg gcaaaccgcc caaaaccacg cgtaccgaca aaacggttac
     2401 gccgtccaaa aaaccgtttc acgcgggcga tatcgacatc accgaagccg cctaccgcgt
     2461 tttgcgcctg cctgccgtag ccgccaaaaa cttcctgatt accatcggcg accgcagcgt
     2521 cggcggcatg acccaccgcg accaaatggt cggcaaatac caaacccccg tagccgactg
     2581 cgccgttacc atgatgggct tcaacaccta tcgcggcgaa gcgatgtcta tgggcgaaaa
     2641 accggccgtc gccctgtttg acgcgcccgc ctcgggcaga atgtgcgtcg gcgaagccat
     2701 caccaacatc gcggcagtca acatcggcga catcggcaac atcaaacttt ccgccaactg
     2761 gatggcggcg tgcggcaacg aaggcgaaga cgaaaaactc taccgcaccg tcgaagccgt
     2821 ttctaaagcc tgccaggcat tggatttgag cattcccgta ggtaaagaca gcctgtcgat
     2881 gaaaaccgtg tggcaggacg gcgaagagaa aaaatccgtg gtttcgccgt tgagcctgat
     2941 tatttccgcg ttcgcgcctg ttaaagacgt gcgaaaaacc gttacgcccg aattgaaaaa
     3001 cgtcgaaggc agcgtattgc tgtttatcga tttgggcttc ggcaaagcgc gcatgggcgg
     3061 ctcggcgttc ggtcaggtgt acaacaacat gagcggcgac gcgcccgatt tggacgatgc
     3121 aggccgtctg aaagcgtttt acagcgtaat tcaacagctt gtcgccgaag acaaactttt
     3181 ggcgtatcac gaccgcagcg acggcggttt gtttgcgacg ctggcggaaa tggcgtttgc
     3241 ggcacggtgc ggtattagtg ccgatataga ttgcctgatg gataaattcc tgccgattca
     3301 tctcccggat tttcaaggcg accctgccga agacttatct gacgaacttt ataatcatgc
     3361 cgccattaaa atcttattca atgaagaatt aggcgcggtt atccaaatcc gccaaaaaga
     3421 tagggattat gttgatgcgg catttgaaac ggccggctta accgatgcgg tcagccggat
     3481 tggctctcct gattttgaca atgagtttat ttctttcttt ggttacggtt atttcttgga
     3541 acaaaaccgc gccgacctgc aacgcgcgtg gcaggaaacc agccacgcca tccaacgcct
     3601 gcgcgacaac cctgcctgcg ccgacagcga gttcgccctg attggcgaca acgaacgcag
     3661 cgcattgttt gccgacgtga agttcgacgt gaacgaagac atcgccgcgc cgtttatcaa
     3721 cagtggcgcg aaacctaaaa tcgccatcct gcgcgaacag ggcgtaaacg ggcaaatcga
     3781 aatggccgcc gccttcaccc gcgccggatt cgatgcctac gacgtgcata tgtccgacct
     3841 gatggctggc cgcttccgcc ttgccgactt caaaatgctg gcggcgtgcg gcggcttcag
     3901 ctacggcgac gtactcggcg cgggtgaagg ctgggcgaaa tccatcctgt tccaccctgc
     3961 cttgcgcgac caattcgccg ccttctttgc cgatccgaac acgctgacat tgggcgtgtg
     4021 caacggctgc caaatggtca gcaaccttgc cgaaattatc ccgggcgcag aaacatggcc
     4081 gaagttcaaa cgcaacctga gcgagcagtt tgaagcgcgc ctgaatatgg ttcacgtccc
     4141 caaatcagcg tcgctgattt tgaacgaaat gcaagactcc agcctgcctg tcgtggtcag
     4201 ccacggcgaa ggccgcgccg acttcgcgct tcacggcggc aatatttccg ccgatttggg
     4261 catcgcgctg caatatgtgg acggtcaaaa ccaggttacc caaacctatc cgctcaaccc
     4321 caacggctcg ccgcagggca tcgccggcat taccaacgcc gacggccgcg ttaccattat
     4381 gatgccccac cccgaacgcg tgtaccgtgc cgcacaaatg agccggaaac cggaaggctg
     4441 gacggaactg tccggctggt accgcctctt tgccggcgca cgtaaagcct tgggctaaac
     4501 cgtctgccca aacaaatgcc gtctgaaaaa ggttttgaaa cccgtttcag acggcatttt
     4561 gaccattctc aagccctgcc ggtactcaat cccgctttac cgttttcccg caaagttttc
     4621 agcaatactt tccacgccga caatttaggg ttggactgtt ttccttgtcc gatcaaatcc
     4681 atttgcgtca tataccagtt catttctaac attgcacccg cttttttatc aacaaatgct
     4741 acgccagttt ggatgcggtc tgcttgtacg gtttggtaag ttctgttttg cattttgttc
     4801 gccaaatcag ggactaaggc aaacgggcgg gctatgccga ccaaatccaa atgaccgctg
     4861 gataaggcat cttccatggc ggtttgcgaa cggaatccgc cggtgataat cagcggggct
     4921 tggctggctg cacgggcttt ttcggcgtaa tcgatgaaga aggcttcgcg tttgcgggtg
     4981 ctgtctttgg cggcgagcat ttgcgggctt tcgtagttgc caccggaaac ttcaataaaa
     5041 tcaatgccca tttccgacag tttttgcacc acctgcaccg attcgctttc gtcaaatccg
     5101 cctttttgga aatctgccga attgagtttt acgcccacca agaaatcttt gcccgcggca
     5161 gcgcgaatag cggtgtaggt ttccaaaagg aagcgcatac ggttttccaa actgccgccc
     5221 cattggtctt ggcggcggtt gtggtgcggc gagaggaatt ggctgatgag ataaccgtgt
     5281 acggcgtaaa tttgtacgcc tgaaaagcct gcctgttcgg caattttggc agtttgtacg
     5341 aattgctgaa tcagcccgtt gatttcatcg gcactcaatt cgcgcggcgg attgataaag
     5401 ccattcatgc ccaccagcgg cacggcgctc ggtgcaagcg gtgttttatt gaccaccgca
     5461 ggcgactgtt tgcccgcatg attgatttgc atgatgagca gcgtgtcgtt ttgcatgcgg
     5521 gctttcgccc attttttcag catttcaagg gcgcggtcgt ctgaaatcaa cacatcgttt
     5581 atcgaacctt tgccgctttc agccaccatc acattgcccg tcaccaaaac gcctgcgccg
     5641 ccttctgccc aagcgccgta caggcggaca agtttttcag acggctggtc gttttgggca
     5701 agttgctctt ccatggcgga tttgaagata cggtttttgg cggttttgcc gttttgaaaa
     5761 gtgaatggcg tgaataacat gatcgttcct ttaagttgat aagtttaaaa atgtaaacgt
     5821 atttgggtaa aaataaaggg ccaacggata accctatttt gaaagcgggc aattttgagc
     5881 catcaaatcg gcaagctgtt tcaatctcgc caccgcatcc gccatggcaa tactgtaaaa
     5941 ccgctctttg ccgacctgct ccaccgccac cgctccggct tgcagcatga tttttaaatg
     6001 gtgcgacaca gcagggcgcg acagatgcag atgctcggtc agctcattca cattcatcct
     6061 accgtgtttc cacaatacat gcaggatctg atggcggttt tcatcgctca atacggtaaa
     6121 aatgggtatg cactcgcgca ttaaattcat ggtttgttgc ggcatattcg atctattgag
     6181 tttatatatt taaaaattcg aacgtatttt agagggaagg tcggcagtta tcaaaggcag
     6241 tttggtttgc agtttggcaa tatattgttg ctgaaaggtt tttataagaa aaaatgccgc
     6301 ctgaaacagc atgatggcgt ttgctagggt tccgctgtat attaacgcct ttcggataca
     6361 cctttcagac ggcatccgaa ccatcaagga acactcatga aaattacccc cgtcaaagcc
     6421 ctgaccgaca actacatctg gatgatacag cacggcaacc atgccgtctg cgtcgatcct
     6481 tccgaaccct cgcccgtctt ggaattcctc gtacgcaacc gactcatgct cgcccaaacc
     6541 tgggtaacgc acccccatcc cgaccacgaa ggcggtgcgg cggcactctg gcgcggctac
     6601 atggaatcgc ccgtttacgg cgaatccgac atcgaagccg ccacccacac cgtaaccgcc
     6661 ggcacacgat tcaccttcgg caacggtcag gttaccgttt gggcaacacc cggccacaca
     6721 gaccgccaca ccagctacct tctcgaaact tcagacggca tacacgtttt ctgcggcgac
     6781 accctttttt ccgccggctg cggacgcgtg tttaccggca cggtcgaaca gctttacgac
     6841 aacttccaac ggttcaacca attacccgaa ggcaccctgt tctatccggc acacgaatac
     6901 accgccgcca acctgcgttt cgccgcccat atcgagccgg acaacgccga cattcagacg
     6961 gcattgaagg cggcggaaca cacgcccacc ctgcccgtta ccctcgcgca cgaacgccgc
     7021 gtcaacccgt tcttacggac agaaatcccc gccgtccgcc aacgtgccga agccctggtc
     7081 ggcaaaacgc tgaacagcgg tttggaagta ttcgccgccc tgcgcgaact gaaaaacgcc
     7141 taccgctgac ctgccctccg aaaaatgccg tctgaaaccc gcgtttcaga cggcatttgc
     7201 gttaaaaata gtaaaccgtt tcaaaaggga gtagaatagt gctgtttcca accctgcgcc
     7261 tgtaccgtca ggcttttatt atggaccttc ccagttcgtt tttactgaac accccttccg
     7321 attccaaacg acaataacca tcccgccgga atgcctcccc gcacacggcg ggcggagcat
     7381 ttatgagcat cgaacacact cctccgacac acgacggcga aaccggtcaa aaccatgccg
     7441 aacgcccttc cgccgatttc gaccgtgtcc actccctctg cgaaatcctc gaacctgctt
     7501 ttgaacaaat cgaaaacggc acaccgctcg aagatgcgcc gctgcgcgac aagctgaccg
     7561 agctgaccgt cctcttggcc gagttgcacc ctgccgacgt ggccgacgtc ttggagtccc
     7621 tgccgccgcg cgaacgcaat atcgtttggc ttctggtcaa acccgaagac gacggcgaag
     7681 tattgctgga agtatccgac gcggtgcgag aaacgctgat cgagtcaatg gacaaggacg
     7741 aattgttggc ggcggtcgat gatttggatg cggacgaatt ggcggagctg gcagacgatt
     7801 tgccgcacca agtggtttat gaagccttac agacgcgcga tgaggaagag cgcgcccaag
     7861 tcaaggcggc aatgtcgtat gaagacaacc aagtcggtgc gattatggac ttcgagttgg
     7921 tcagcatccg cgccgatgtc gcctgcgaag tggtgctgcg ttatctgcgc cgcttcgaca
     7981 gcctgcccga ccataccgac aagatttttg tcgtcgatga aaacgacgtg ctgcagggcg
     8041 tgctgcccat ccgcaaactt ttggtcgccg atcccgaaga tttggtggaa aacgtgatgg
     8101 cgaaagatgt cgtgcgtttc cgcgccgagg acgacgtgga agaagcggcg caggcgtttg
     8161 aacgctacga cttggttacc gcgcccgtcg ttgatgaaaa caaaaagctc atcggcagga
     8221 ttaccatcga cgagatggtg gacgtgatcc gcgaagaatc cgaagcggat atgttgaaca
     8281 tggcaggttt gcaggaagag gaagacctgt tcgcccccgt cttggattcc gtcaaaaacc
     8341 gctggatgtg gctcgccgtc aacctctgca ccgccttcct cgccagccgc gtcatcggcg
     8401 cgtttgaagg cagtatcgaa aaaatcgtcg cactcgccgc gctgatgccc atcgtcgccg
     8461 gcatcggcgg taactcgggc aaccagacga ttaccatgat tgtccgcgcg atggcgatgg
     8521 ggcagctgac ggatatgcag gcgggaaggc tgttgaaaaa agaagtcggc gtcgccttgg
     8581 tcaacggcat tatttggggg acggtgatgg gagcggtttc gtggctgctt tacggcagcc
     8641 tcggcatcgg tttggtcatg gttgccgcga tgacgctcaa cctcctgctg gcggcaacgg
     8701 tcggcgtatt gattcccgtg gtaatggaaa aattcggacg cgatcccgcg ctgggcagct
     8761 cggtgctgat taccgccgtt accgactccg gcggcttcct cattttcttg agtctcgcca
     8821 ccctgttcct gctttaaatg ccgtcccgac ccgcgcaaaa atgccgtctg aagcagaagc
     8881 tgcttcagac ggcattcgac cattcatcct tgttgctcaa gattattgga cggtatgtcg
     8941 gggcagccct ttggcaacgc cgaccacatc ctccccgaac agcgcgttga catcggtttc
     9001 atcaaacacg tatttgctgt ggcagaagtc gcaatcgact tcgatgctgc cttgttccgc
     9061 caccacacct ccgacttctt ccccgcccag catcaacagc atatcgctga ccttgccccg
     9121 agaacaggta catgaggatt caaacgtttc cggctcgaac acgcgcggcg gcgtttcgtg
     9181 gaacaggcgg tataaaacgt gttgcgcgtc caattctgcc agctcctccg ccgtcagcgt
     9241 gcgcgccagc gtactgacgt gttcccatgc ctcttcatcc aacacctctt caggcagacg
     9301 ctgcaccagc agcccgcccg ccgcttcatc gtttgcagac agggcgatgt gcgtatcaag
     9361 ctgttcggaa cgtttcatat agttcaccaa catttgcgcg atgctgccgc cttccaaagg
     9421 cactacgccc tgccagggtt cgccgtcttt gggttgcagc gtcaatacga acacgccgtt
     9481 gccgcccaaa aggtcgccga ggctttcgtc atcggctatt tctgcggttt cgtcccaccg
     9541 cgcggttgca cggacggtac ggtcggaagt cgcttccgca accagcattt tcagttttcc
     9601 ctgcccctga acctgcacaa tcagcgtgcc ttcgttttta aggttgcccg acagcagaac
     9661 gccggcagcc agtagctctc ccaaggcgca gctgatggcg gcgggatagt ttttctgttt
     9721 tacaatgtgc tgccacacgt tttccaaacg gacgtgcagc ccgcgcacgg gcatatcgtc
     9781 gaagataaag cgggtacgcg catcggcgcg gttgatggcg gtttgattca tgattttctc
     9841 tgactgattg ttcggatggc ggctatatgg ttgcggtcgg cgcgaaaaca aggcggacgg
     9901 cggatgcgct tcccaaattc tcaataaatt atataaaaat caacatatta actcaatcta
     9961 acaagccgtt ttttgccaaa cagccgtttt tttatataca atcaacaaga tattttcgac
    10021 tgatacagca taacatcgca cggcggcacg atgcctcctg cgcggaaaca ccgatatgga
    10081 ttcttttttc aaaccggcag tttgggcggt tttgtggctg atgtttgccg tccgccccgc
    10141 ccttgccgac gagttgacca acctgctcag cagccgcgag cagattctca gacagtttgc
    10201 cgaagacgaa cagcccgttt tacccgtcaa ccgagccccc gcccggcggg cgggcaatgc
    10261 cgacgaactc atcggcagcg cgatggggct tttgggtatt gcctaccgct acggcggcac
    10321 atcggtttct accggttttg actgcagcgg attcatgcag cacatcttca aacgcgccat
    10381 gggcatcaac ctgccgcgca cgtcggcgga acaggcgcgg atgggcgcac ccgttgcccg
    10441 aagcgaattg cagcccgggg atatggtgtt tttccgcacg ctcggcggca gccgcatttc
    10501 ccatgtcgga ctttatatcg gcaacaaccg cttcatccac gcgccgcgca cggggaaaaa
    10561 tatcgaaatc accagcctga gccacaaata ttggagcggc aaatatgcgt tcgcccgccg
    10621 ggtcaagaaa aacgacccgt cacgctttct gaactgattt cccaaggaat acgcaatgag
    10681 tatgcccgaa atgcccaaat ggtacggcga tgacggacag atcgtatcct gtaccgaaaa
    10741 ggtcaaagtg atgtccgaaa atatggccga gctgtatcag acggcacaag acgcgtttga
    10801 agacgcgctg ctgatgggtt gcggcgaacg tcagttgcgc gcttacctgc tcgcgctgat
    10861 tgaaggtttg gaaaatccct accgcaaagt ctgaacacgc cccggttgct gcggcacggt
    10921 ttatccgtgc cgtttttgcg tttgtgcgcg gcttcggctt ttcagacggc atatttgacg
    10981 ttatgattaa acagttaaca agatttatca caacgccgtc aaaagacaga cacacaacat
    11041 gaacatcatc cgcgcgctcc tcatcatcct cggctgcctc gccgccggcg aaaccgccgt
    11101 tttcctagca ggcatcaaac tgcccggcag catcgtcggc atgggcgtgc tgtttgcgct
    11161 tttgcaggcg ggttgggtca aaacgtcttg gctgcaacag cttaccgacg cgctgatggc
    11221 aaacctgacg ctgttcctcg tgccgccctg cgtggcggtc atcagctatt tggatttgat
    11281 tgccgacgat tggttttcga tactggtttc cgcctccgcc agcactttgt gcgtactgct
    11341 ggttacgggc aaggttcacc gctggatacg gagcattatc tgatgagcga aatcctcagg
    11401 cagcccggca tcctgctttt cctcacgctt gccgtgtacg cgcttgcgat tatcgtgcgc
    11461 acgcgcacgg gcaatatctt ctgcaacccc gtactcgtca gcactatcgt gctgattgcc
    11521 tacctgaaaa tcctcggtat cgattatgcg gtgtaccaca acgccgcgca attcattgat
    11581 tttcggctga aacccgccgt cgtcgtgctt gccgtgccgc tctaccaaaa ccgccgtaaa
    11641 atcttcaacc agtggctgcc cgtcatcgtt tcgcagcttg cgggcagcgt tacgggcatt
    11701 gttacgggga tgtattttgc caagtggctg ggcgcggaac gcgaagtcgt cctcccgctc
    11761 gcgtccaaat ctgttaccaa ccccatcgcc atcgaaatca ccagctccat cggcggcatt
    11821 cccgccatta ccgccgccac cgtcatcatt gccggtctgg tcggacagat tgccggttac
    11881 aaaatgttga agaacacggt cgtcatgccc tcgtccgtgg gtatgtcgct cggcacggct
    11941 tcgcacgcga tgggcattgc cgcctcgctc gaacgcagcc gccgcatggc ggcatacgcg
    12001 gggctggggc tgacgttcaa cggcgtgctg accgcgctga ttgcgccgct gcttatcccc
    12061 gttttggggt tctgaacccg tttcagacgg catttcaacc catgccgtct gaacgccgac
    12121 acactcgcaa ggagaaccgt tatggctgtc aacctgaccg aaaaaaccgc cgaacaactg
    12181 cccgacatcg acggcattgc cctctacacc gcccaagcag gcgtgaagaa gcccgggcat
    12241 accgacctga cactgattgc cgtagccgcc ggcagcaccg tcggtgcagt cttcacgacc
    12301 aaccgtttct gtgccgcgcc cgtccacatc gccaaatcgc accttttcga cgaagacggc
    12361 gtgcgcgccc tcgtcatcaa cacgggcaac gccaacgcgg gtacgggcgc acagggcaga
    12421 atcgatgctt tggcagtgtg tgccgccgcc gcccggcaaa tcggctgcaa accgaaccag
    12481 gtgctgccct tctccaccgg cgtgattctc gaaccgctgc ccgcagacaa aatcatcgcc
    12541 gccctgccca aaatgcagcc tgccttctgg aacgaagcgg cacgcgccat catgaccacc
    12601 gacaccgttc ccaaagccgc ctcgcgcgaa ggcaaggtcg gcgaccaaca caccgtccgc
    12661 gccacaggca ttgccaaagg ctcgggcatg attcatccca atatggcgac catgctcggt
    12721 ttcatcgcca ccgatgccaa agtttcccaa cccgtcctcc aactgatgac gcaggaaatc
    12781 gccgacgaaa ccttcaacac catcaccgtt gacggcgaca ccagcaccaa cgacagcttc
    12841 gtcatcatcg ccaccggcaa aaacagccaa agcgaaatcg acaacatcgc cgacccgcgt
    12901 tacgcccaac tcaaagaatt gttgtgcagc cttgcgctcg aactcgccca agccatcgtc
    12961 cgcgacggcg agggcgcgac caagttcatc accgtccgcg tcgaaaacgc caaaacctgc
    13021 gacgaagccc gccaagccgc ctacgccgcg gcacgttcgc cactggtcaa aaccgccttt
    13081 ttcgcctccg accccaacct cggcaggctg ctcgccgcca tcggttatgc cgacgttgcc
    13141 gacctcgata ccgacctcgt ggaaatgtat ctcgacgata ttttggttgc cgaacacggc
    13201 ggacgcgccg caagctacac cgaagcacaa gggcaggcgg tgatgtcgaa ggacgaaatc
    13261 accgtccgca tcaagctgca tcgcggacaa gccgccgcca ccgtctatac ctgcgacctg
    13321 tcgcacggat acgtttccat caacgccgac taccgttcct gacccgacac ggcttcagac
    13381 ggcatacata aaatgccgtc tgaaccgtcg gacaacatac catgacctcc acattccccc
    13441 gccgcctcgc ccgcaaaatc cgccaaaccc gccgcctgtc gcgcaaaagc atcgcctttc
    13501 tgttccttct ggcaggttcg gcactcgtcg ccctgaccgc actgtttttt gcccatcttg
    13561 ccgattttgc gctggaactg aacgccaaac tggttcaaca atacccgtgg ttcgcatgga
    13621 ttgccctgcc gccgggactg cccgtcatcg tctggctcac acgcaaattc gcccccttca
    13681 ccgccggcag cggcatccct caagtcatcg cctcactgtc gctgccctac ggcgcacaaa
    13741 aaacgcggct gatacgcctc gggcaaacgc tgctgaagat tccgctgacc tttttgggta
    13801 tgctgttcgg cgcgtccatc ggacgcgaag gcccgtccgt gcaggtcggc gcggcagtaa
    13861 tgggcgcgtg gggcgcgtgg tgcaaaaaac acggtttggc gtttaagggt atgcaggaaa
    13921 acgatttgat ggcggcgggc gcggcgggcg gtttggcagc agcgttcaac gcgccgctgg
    13981 cgggcgtgat tttcgccatt gaggaactgg ggcgcggcat catgctgcgc tgggagcggc
    14041 agattctgct cggcgtactc gccgccggtt tcatacaggt cgccattcag ggcaacaacc
    14101 cgtatttttc gggtttcaac ggcggcgttt tggaaaatat tttgatgtgg atcgtggcgg
    14161 cggggctggt ttgcggcgcg gctggcggca tatttgcccg tatgctctat cgcggtgcgg
    14221 cggcgtttgc accgcgcaag atacgcggct tcatccgcaa ccgtccgctg ctgctggcgg
    14281 cactgatggg gctgctgctc gccctgctcg gcacgttcta ccaaggcaaa acctacggca
    14341 ccggctacca cgaagccgcc caagccctgc acggcatcta cgaagccccc gtcggactcg
    14401 ccgccgccaa atggctcgcc accgtattca gctattgggc aggcatcccg ggcggcattt
    14461 tcactccctc gctgaccata ggtgcggttt tgggcgagca tatcgccgcc atcgccgaca
    14521 tatcgcaggg tgcaaacatc atcgtcctca tctgcatggc ggcattttta gcgggcgcga
    14581 cacaatcgcc gattacttcc gccgtcgtcg tcatggaaat gaccggcggg caaagcctgc
    14641 tgttttggat gctgattgcc tgcattttcg cctcgcaggt ttcgcgccag ttttcgccgc
    14701 gtccgttcta ccacgcatcg ggaatgcgct tccgccggcg cgtgcttcag gaaaccgccg
    14761 cccaaaccgg caatgcgccc gcaagaccgc aagccgcaaa cagcaaaacg ggaatgccgt
    14821 ctgaaaatta aaacgccccc gatcaaacgc cggcagccgc cttgattcaa ataccgttcc
    14881 gctgcgcttg aaatttcagc aacaatgccg tctgaacgac agaatgcggt tttcagacgg
    14941 catttcccca tcccgatatt gctcaaacaa aaccgaagcg tttgctataa ttgcactttt
    15001 taccgcacac gcacgcccat gtttcccgat ttctcccaaa ccctctccaa agaccgccac
    15061 ttcctgcaat ccgccttcaa aaatcccaac aaatacggcg gcttgtccaa aatcgaagaa
    15121 aaataccgaa aatcgcacga aatcttcttg aaacgtttgg ccgcattgcc aaaacccgaa
    15181 ttcgacgaca ccctgcccgt tcacgaaaaa ctcgaagaaa tcaaaaaaac cgttgccgaa
    15241 aatcaggtaa cgattatctg cggcgaaacc ggttcgggca aaaccacgca gttgcccaag
    15301 atttgtttgg aactcgggcg tggggcggca ggattgatcg ggcataccca gccgcgccgt
    15361 ttggccgcgc gttcggtggc ggagcggatt gccgaagagc tgaaatccga aatcggcagc
    15421 gcggtcggct ataaagtgcg ctttaccgac cacacctcgc gcgatgcctg cgtcaagctg
    15481 atgaccgacg gcatcctgct ggcggaaacc cagaccgacc gttatctcgc cgcctacgac
    15541 acgattatca ttgacgaagc gcatgaacgc agcctgaaca tcgacttcct cttgggctac
    15601 ctaaaacaac tcctgccgcg ccgccccgat ttgaaagtca tcatcacatc agccaccata
    15661 gacgcagaac gcttctcccg acacttcaac ggcgcgcccg tactggaagt gagcgggcgc
    15721 acctatcccg tcgaaatcct ctaccgaccg ctgaccggca aagacgaaga cgacgcagaa
    15781 gtcgagctga ccgacgcgat tgtcgatgcc gccgacgaat tggcacgcta cggcgaaggc
    15841 gatattttag tattcctgcc gggcgaacgc gaaatccgcg aagcggcaga agccctgcgc
    15901 aaatccacac tgcgccgcaa tgacgaaatc ctgcccctgt ttgcacgcct gtcgcacgcc
    15961 gaacagcaca aaatcttcca cccttcaggc gcaaaatgcc gcatcgtgct ggcgaccaac
    16021 gtcgccgaaa cctcgctcac cgtgccgggc atcaaatacg tcatcgacac cggcctcgcg
    16081 cgcgtcaaac gctattccgc gcgggcaaaa gtggaacaac ttcacgtaga aaaaatctcc
    16141 caagccgccg cccgccaacg ctccggccgc tgcggacgcg tctcagcagg cgtgtgtatc
    16201 cgactgtttt cagaagaaga ttttaacagc cgtcccgaat ttaccgatcc cgaaatcgtc
    16261 cgcagcaacc tcgccgccgt catcctgcgc atggcatcgc tgaacttggg cgacgtagca
    16321 gcattcccat ttttagaaat gcccgattcg cgatatatca atgacggttt tcaggtgttg
    16381 ctggaattgg gggcagtgga ggagaattag attgaaagca ggctacctga aaatgtgaag
    16441 cagcctgcac ttttaaggtt aaattgaata aagttcagat gacttaaacg tagaaaacca
    16501 agcggtcagc taactgacgg cttggttttt gacccacccc tgaccctaat tcttacaacg
    16561 gtgtttcagg tattcagttt ccctttctcc tagctgtatt cacagcaaca gttgtaagcc
    16621 gagattttga tttacctttg cgctatgaat gcaaacaggc atttcatagg gcttaatcaa
    16681 taatcatgat gataaatcgt caggcttaat attgcccaat gaatagcatt ttatttaagg
    16741 ctgctttcct gccttggggg tttaaggtat tttaccatga agcctgatat tcaataatat
    16801 cgtttacaga gaatccatga tttaacaagg cttgccgaat aagaaaaggt aaagattgag
    16861 tatatttagg gaagttacaa caaaattcca accaaaacct gcccatattt tcaatatctt
    16921 tactacttaa tttatcaaaa aatcgaagta actcgctaat tcctattgat tgatttaaaa
    16981 tagtttgcca tgctgctgct tccaaacttt tatcataatc gtcataaatt ttctttttta
    17041 aaatcatata aaattgttca t
//
LOCUS       DAWTHD010000044        16939 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWTHD010000044 DAWTHD010000000
VERSION     DAWTHD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.044"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(86..418)
                     /locus_tag="WM704_001098"
     CDS             complement(86..418)
                     /locus_tag="WM704_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HFA9340532.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            597..1433
                     /gene="cysT"
                     /locus_tag="WM704_001099"
     CDS             597..1433
                     /gene="cysT"
                     /locus_tag="WM704_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFA9340533.1"
                     /translation="MFYVTAQNTRRTARLQTQPRSDRIVPVASGGFAVCDDGGGNRLG
                     RLSEHDCRAERAGGGAAEFADVVLCDADQCVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLKELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGSIPMISEILPLIITGKLEQFDAQGASAVALFMLLVSFVILFALNVLQWALGRRSGA
                     KG"
     gene            1622..2482
                     /gene="cysW"
                     /locus_tag="WM704_001100"
     CDS             1622..2482
                     /gene="cysW"
                     /locus_tag="WM704_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFA9340534.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAVLGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPAVAGLMFVLLFGAHTALGSRLEAQGIRIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            2479..3555
                     /locus_tag="WM704_001101"
     CDS             2479..3555
                     /locus_tag="WM704_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFA9340535.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IHPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(3611..5137)
                     /gene="ilvA"
                     /locus_tag="WM704_001102"
     CDS             complement(3611..5137)
                     /gene="ilvA"
                     /locus_tag="WM704_001102"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFA9340536.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGMAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            5286..6455
                     /locus_tag="WM704_001103"
     CDS             5286..6455
                     /locus_tag="WM704_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFA9340537.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            complement(6581..7153)
                     /locus_tag="WM704_001104"
     CDS             complement(6581..7153)
                     /locus_tag="WM704_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFA9340538.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(7220..8203)
                     /locus_tag="WM704_001105"
     CDS             complement(7220..8203)
                     /locus_tag="WM704_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFA9340539.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(8371..8446)
                     /locus_tag="WM704_001106"
     tRNA            complement(8371..8446)
                     /locus_tag="WM704_001106"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8411..8413),aa:Gln,seq:ttg)
     gene            complement(8459..8534)
                     /locus_tag="WM704_001107"
     tRNA            complement(8459..8534)
                     /locus_tag="WM704_001107"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8499..8501),aa:Gln,seq:ttg)
     gene            complement(8542..9387)
                     /gene="ispE"
                     /locus_tag="WM704_001108"
     CDS             complement(8542..9387)
                     /gene="ispE"
                     /locus_tag="WM704_001108"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFA9340540.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9397..9978)
                     /locus_tag="WM704_001109"
     CDS             complement(9397..9978)
                     /locus_tag="WM704_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization factor LolB"
                     /protein_id="HFA9340541.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDRDGALAVDGKGNVYQAEGTE
                     DLSRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(10118..11956)
                     /locus_tag="WM704_001110"
     CDS             complement(10118..11956)
                     /locus_tag="WM704_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340542.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            complement(12283..13119)
                     /locus_tag="WM704_001111"
     CDS             complement(12283..13119)
                     /locus_tag="WM704_001111"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFA9340543.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            complement(13242..14033)
                     /gene="panB"
                     /locus_tag="WM704_001112"
     CDS             complement(13242..14033)
                     /gene="panB"
                     /locus_tag="WM704_001112"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFA9340544.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKVHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(14175..14250)
                     /locus_tag="WM704_001113"
     tRNA            complement(14175..14250)
                     /locus_tag="WM704_001113"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14215..14217),aa:Thr,seq:tgt)
     gene            14416..15207
                     /locus_tag="WM704_001114"
     CDS             14416..15207
                     /locus_tag="WM704_001114"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFA9340545.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            15262..15903
                     /locus_tag="WM704_001115"
     CDS             15262..15903
                     /locus_tag="WM704_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9340546.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15982..16905)
                     /locus_tag="WM704_001116"
     CDS             complement(15982..16905)
                     /locus_tag="WM704_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFA9340547.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILFVDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLRPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTKTDLPYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
BASE COUNT         4028 a         4953 c         4072 g         3886 t
ORIGIN      
        1 tccgcccatt gcaaacagat gcaatccgcc gattatttaa aaaacggcaa agccttgcac
       61 cccttgcggc aagcctgcca tgcctttaat gtccgcaggc gcaagcgtcg ccgtggtgtc
      121 cgcaatcttt agccgcttcg agccacgcgt cgtcatcgcc gtcaaactct tccacttcgt
      181 caaactcgcc gcgttccacc atcgcgcaca actcttcaag gctttccgcc ccttccaccc
      241 aaaaacaggg aatgcccggc ggcgtatcgg gcgcaagcag tgccgccacg ccttcatgcc
      301 acgcaatcca atagccgttg tccgtacgga caaattgtgc gttttcgttg accgactcgc
      361 cttccgtacc ttccagcata cgttccgcaa tgtccgcctg aaaagataaa atctgcataa
      421 gtgttccttt atatgatggt tttccgtcaa aacaaagcgt tattttagaa catcgcgccc
      481 gcttgaaaaa ctgccaaccg ctttataaca aattcgtctt tgcaccaaac tttccatttt
      541 ttccgttttt caggcggcat tgttaaagta gtccttcctt ttccttattt tcagcattgt
      601 tttatgttac cgctcaaaac acccggcgta ctgcccggct tcaaactcag cctcggtctg
      661 accgtattgt gcctgtcgct tctggtggtt ttgccgtttg cgatgatggc ggcggaaatc
      721 ggctggggcg gctttcggaa cacgattgcc gagccgaacg tgctggcggc ggtgcggctg
      781 agtttgcgga tgtcgtttta tgcgatgctg accaatgtgt cgtgttcggc acactggtgg
      841 cttgggtgtt ggtgcgctat gaatttccgg gcaagggcct ggcgaacgcg ctggttgatt
      901 tgccgtttgc gctgccgacg gcggttacgg gtatcgcgtt ggcggccctg tatgcgccca
      961 acggttggat aggccgtttt ttcgaacctt tgggcatcaa aatcgcgttt acgcccgtcg
     1021 gcatttggat tgcgctggtt gtcgtcagcc tgccctttat cgtccgcgcc gtgcagccgg
     1081 tattgaagga attgtcgggc gaatatgagg aagcggcggc gactttgggc gcaaaccgtt
     1141 tcacaacgtt ccgccgcgtg ctgctgccgg aaatcacgcc ggcgctcttg accggcgcgg
     1201 gcatgatgtt cgcgcgggca acgggggaat acggttcggt gatttttatc gcgggcagca
     1261 ttccgatgat ttccgaaatc ctgccgctga ttattacggg caagctggaa caattcgacg
     1321 cgcagggcgc gtcggcggtg gcgttgttta tgctgctggt ttcgtttgtg attctgtttg
     1381 cgctgaacgt gctgcagtgg gcgttgggca ggcgttcggg tgcgaagggt tgaggtcgtc
     1441 tgaaaacgaa tccgctcccc aaaacacaga attcgtaggt cggatacttg tatccgacaa
     1501 acatttgcga ttcagcaacc ttgtcggatt caagaatccg accgacgaaa gctgaaaccc
     1561 ctcaatccct ttcagacggc atccgaacac cttaaacaac aaaccagaga acaccaccgc
     1621 catgaaaccc tattccgcca atcccaacct gaccgaaccg cgccggctgc gcatgttgct
     1681 gattgccgcc gtgctgggct ttctgctgct gatgctggtc gtgccgctcg tcgccgtgtt
     1741 ttacgaagcc ttaaaaggcg gttgggattt gtacctgaaa tccttaagcg atcccgaagc
     1801 gtggtctgcc gtcaaattga cgctgattac cgcgctgatt gccgttcccg tcaatgccgt
     1861 cttgggcgtg gcgatggcgt ggctgttgac ccgttttgac tttctcggca agcagttgct
     1921 gaccaccctg ctcgatttgc cgttttccgt atcgcccgcg gttgccggtt tgatgttcgt
     1981 cttattgttc ggcgcgcaca cggcgttggg cagccggctg gaagcgcaag gcatacggat
     2041 tatcttcgcc attcccggca ttgttttgac ggcgctgttc gttacctttc cctttgccgc
     2101 acgcgaaatc atccctttaa tgcaggcaca gggcgacagc gaagaacagg cggcattgat
     2161 actcggcgca agcggttggc agatgttttg gcgcgttacc ctgcccaaca tcaaatggac
     2221 gctgctctac ggcatcatcc tcaccaacgc ccgtgcgatg ggcgagttcg gcgcggtcag
     2281 cgtggtatcg ggacacatac gcggcgaaac caacactatc ccgcttttgg tcgaaatctt
     2341 ctacaacgaa tacaacttca ccggcgcatt ctccctctcc ggcgtattgg cacttttagc
     2401 actggcgacg ctggcggtgc agaacatcat caccaaatta caagacaaaa aactcgccgc
     2461 cgccgaaagg aacgcagtat gagtattacc attcaaaact taaacaaaaa acgcttcggc
     2521 aattttcacg cgctgaaaaa catcaacctc aacgtccccg ccggcaaact cgtttccctg
     2581 ctcggtccct ccggctgcgg caaaaccaca cttttacgca ttatcgccgg actggaaaac
     2641 gccgacggcg gccaaattct ctttgacggc caagacgtaa ccgccaagca tgtgcgcgag
     2701 cgcaaagtcg gtttcgtgtt ccaacactac gccctcttcc gccacatgaa tgtgtttgac
     2761 aacgtcgctt tcggtttgac cgtattgccc aagcccgaac gcccgtccaa aggacaaatc
     2821 cgcgccaaag tcgaagaatt gctcaaactc gtacaactct cccacctcgc caaatcctat
     2881 ccgcaccaac tctccggcgg gcaacgccag cgcatcgccc tcgcccgcgc gcttgcggtc
     2941 gaaccgaaac tcctgctttt ggacgaaccc ttcggcgcgc tggatgccaa agtgcgcaaa
     3001 gaattacgca cctggctgcg cgacattcat cacaacctcg gcgtaaccag cattctggtg
     3061 actcacgacc aagaagaagc cctcgaagtt tccgacgaaa tcgtcgtgat gaaccatggc
     3121 aaaatcgaac aaaccggcag tgccgaagcc atttaccgca aacccgaaaa cgccttcgtt
     3181 accgagttcc tcggcgaagc cgacgcattt gaaggacgca tcgaaaaagg catctggcat
     3241 tacaacggct tcgcgtggaa attggacgcg caatacaaat ggcaggagca aaccgccacc
     3301 ggctatatcc acccgcacga atggcagatc gccgccgaac acgaaacccc gatgatccgt
     3361 gccgaaatcg aaaaaatcca cgccgtcggc gcattgacgc acgttttggt gaaacacggc
     3421 aaacaagacg tgcatatcac actggctggc agcgatgccg cgcgctaccc aatcgccgaa
     3481 ggcaaaaaat taaacttaat tccgaaacag gtttatgttt tttctcaaaa cgagttgatt
     3541 gaatattcga tttaaccctg aaagcgcaat gccgtctgaa aggctttcag acggcattgc
     3601 gtgtcaaacg tcaggcaaga aacagcttgt acgcggcatt ttgcgtttcc tcgtgatagc
     3661 tgtatcccag actttccaag aaaccgtcaa atgcggcggc atcgtgcggc ggcacgtcga
     3721 taccgaccaa aatccgcccg taatccgcac cgtggttgcg gtaatggaag agcgtaatgt
     3781 tccaaccgcc ctgcatatgg ttcaaaaagc gcgccaacgc gcccggacgc tccggaaact
     3841 caaaactgac cagacgctcg tgccctactt tgtccgtccg ccctccgacc atatagcgga
     3901 tatggatttt ggcaatctca ttattggtca aatcgacatt gggcaatccc gcctcatcca
     3961 accggctgcc gataaccgcc aaatcctgcg ggcctgccgc ttgaagtccg acaaagatat
     4021 gcgctttttc atcgtcgccg tagcggtagt tgaactcggt aatattccta tttcccaata
     4081 tattgacaaa cttaaggaag ctgccgcgtt cttcggggat ggtaacggca aaaatacctt
     4141 cgttgccctc gcccaattcg ctccgttccg aaacgtggcg caaacggtga aaattcatat
     4201 tcgcaccgct ggtaacggca atcagggttt ggttttccgc accttctcgg gcaatatagg
     4261 ctttcagacc cgccaacgcc aacgcgccgg ccggctcggt aatgctgcgc gtgtcatcga
     4321 aaatatcctt gaccgcgccg caaaccgcat cggtatcgac tgtaatgatt tcatccaaaa
     4381 gttctttgca gaggcggaag gttccgtttc cgacgacttt gaccgccgta ccgtctgaaa
     4441 acagcccgac atctttcaaa tggacgattt cgcctgcttc gaccgactgc ttcatacagc
     4501 aggaatcgtt ggtctgtacg ccgataactt taatttcggg acgaacctgt ttgataaatg
     4561 ccgccatgcc cgccgccaaa ccgccgcccc ctatcggtac gaatacggcg cggattggat
     4621 cgggatgctg gctgacaatt tccatcccca ccgtcccctg tcccgcaatc acatcgggat
     4681 cgtcaaacgg cgcgatatag gtcagccctt cctgttccgc cagctccatc gcataatcgt
     4741 aggcatcgtt gtatgaaaca ccccgcaaaa ccacctcgcc gccacggctt ttaaccgcat
     4801 ccactttaat tttcggcgta gtctccggca tgacgataac ggcacggcag cccaaacgct
     4861 gtgcggacaa cgccacgcct tgtgcgtgat tgcccgcgct tgccgcaatc acgccgcaag
     4921 cgagcgcatc tttcggcaac ttggacattt tgttgtacgc gccgcgtatt ttgaacgaaa
     4981 aaaccggctg caaatcttcg cgtttcaaaa ggatgttgtt tttcaaacgt acagaaaggc
     5041 tgcgtgccgg ttccaaaggc gtttcgaccg ctacatcata gacagatgcc gtcaggatgc
     5101 ggatgaggta atcggaataa ggaaggggcg tgttcataat tcaatgcggg ataatcggtt
     5161 tattaaaatc gcaaaaccca aaaccatacg cccaagacgg cgcgaaagca agaaaaatcc
     5221 gcccgatcag acaccctaag cgtataatcg gcaggctgaa acactcacac aatttagaat
     5281 atttcatgac agcacataaa atcctgcccg tccttcttcc catcatctta ggcgtttctc
     5341 acgcaacggc tgcatcgccc gcgcccaaca gaccgacggt acacgccgcc cccacgctcc
     5401 aaacacccga aaccctcacg gcggcacaca tcgttatcga ccttcaaagc aggcagactt
     5461 tatccgccaa aaacaccaat acccctgtcg aaccggcggc actaacccaa ctgatgaccg
     5521 catatttggt tttcaaaaac atgaaatcgg gaaatatcca atctgaagaa aacttaaaaa
     5581 tacccgaatc cgcatgggct tcagaaggaa gcagaatgtt tgtacgtccc ggcgatacgg
     5641 tcagcaccga caaactctta aaaggcatga ttgccctatg cgcaaacgat gccgccctaa
     5701 cccttgccga ccggctgggc aacggctcga ttgaaaattt tgtgcaacaa atgaacaaag
     5761 aagcccgacg cttgggcatg aagaacaccg tattcaaaaa cccgacaggc ttgggtagag
     5821 aaggacaggt ttccaccgcc aaagacctct ccctgctgtc tgaagcattg atgcgcgact
     5881 ttccggaata ttacccgctg ttttccatca aatcgttcaa gtttgaaaac atagaacaaa
     5941 acaaccgcaa tatcctttta tatagggaca acaatgtaaa cggcctgaaa gccgggcaca
     6001 cagaaagcgg cggctacaac cttgccgtgt catactccgg caacggcagg cacatccttg
     6061 tcatcacact aggttcggaa tcggcggaaa cccgcgcatc ggacaacagc aagctgctga
     6121 accgggcatt gcaggccttc gatacgccca aaatatatcc gaaaggcaaa accgttgccc
     6181 aaatccaaat ttccggaggc agcaaaaaaa ccgtccgcgc aggcttcctc aaagaagcct
     6241 acatcactct gccacataaa gaagcgaaaa tggcagaaca gattttggaa accatacagc
     6301 cgattcccgc cccggtaaaa aaagggcaga ttttaggaaa aatcaaaatc aggcaaaacg
     6361 gacataccat tgccgaaaaa gaaatcgtcg cactggaaaa cgtagaaaaa agaagccggt
     6421 ggcaaaggct ttggacgcgt ctgacagggc agtaatctgc cgtttcaaat atcccgcttt
     6481 tccaacaaat aaagaaatgc cgtctgaaac acggttcaga cggcataaaa caacagggcg
     6541 gtacgtattg catacgcgcc gccctgctga tggaagtaag ttagcgcttt ttaccggtaa
     6601 cggtagcaac agccagattt tcgttacgtt tcagggaaac gctttctaca ccttcaggca
     6661 gtttgatgtc tgacaagtgc aaaatgtcgc cggcaaccac ttcggcacaa tccaaatcca
     6721 agaaagcagg gatattggca ggcaacgcaa gcacttcaac agccgtgttc agcagagata
     6781 cgcgaccgcc ttgcaattta acggcttgag aattttcagc gttaacaatg tgcaggggaa
     6841 cgcggatgcg tacaagttga tcggctttca cagcttggaa gtcgatgtgt tggacttcgc
     6901 ggcggaacgg gtgcatttgg aaatcgcgga cgataacgtc tttggtttcg ccgttcagag
     6961 acaatttaat caacgcagta tggaaagatt ctttttccaa tgcgtaaaat acggttttgt
     7021 gatctacggc aatggcaaca ggctcttgac cttcaccgta cagaataccg gggatttggc
     7081 cttcgcgacg caggcggcgg ctcgcaccag tgccttgtgc ttcacgaaca gaggcttgaa
     7141 tttcataagt catgttaaat actccaagtt aggtaaaatc gccgtcatcg gccgcgacca
     7201 gcttaagacg gcatcggggt tacggcagca acatgctgcc tgtcatcact tcttcattga
     7261 aaagatatga gacggattct tcattgctaa tgcggcggac ggtttcggcc aacaaacctg
     7321 caatcgttac ctgacggata cggtcgcagt ttttagccgc ttcagacaaa ggaatggtat
     7381 cggttacgac cacctggtcg atttcggatg aggcaatacg gctgaccgcc tctccggaga
     7441 atacggcgtg gctggcataa gccaagacac gttctgcacc gcgctctttc aaggcgacgg
     7501 cggctttgca cagcgtattt gcagtgtcaa tcatatcgtc cacaatcaga caggttttgc
     7561 cttgaatatc gccgatgatg ttcatgactt ccgccacatt ggctttcggg cggcgtttgt
     7621 cgatgattgc caagtcggca ttcagggatt ttgcaacggc gcgggcgcgg acgacaccgc
     7681 cgatgtccgg gctgacgacg gtcagatttt caatccgctg ctgcttgatg tcgttcaaca
     7741 aaatcggagt ggcataaata ttgtccaccg gaatatcgaa gaagccttga atctggtcgg
     7801 catgcaaatc gacagtcaaa acacggtcga tacctgccga atacagcata tttgccacca
     7861 gtttggcaga aatcggaacg cggacggaac gcggacggcg gtcttggcgc gcatagccga
     7921 aatacggaat ggctgtggta atacgacctg ccgaagcacg cttcagtgca tccgccatcg
     7981 tcaggatttc catcaggttg tcattggtcg gcgcacaggt cggctgaagg atgaaaacgt
     8041 cgcgcccgcg tacgttttcc aacagttcga cggcaacttc gccgtctgaa aacttggata
     8101 cggaagcatt gcccaaagaa atgtccaaat gcctgacaac acgttgtgcc aattcgggat
     8161 tggcattgcc tgtaaatacc atcaaactgt cgtacgcagc catattctca cctgatttta
     8221 tgtttaactt ccgctcagaa aacacaatgc ttccctccgc ttgtaccaag cccggcatta
     8281 tacctgcaac atccgacaca aacaaaggcg tttcaatatt tttatttcta tgaaataaaa
     8341 agcgtgtaag caggcttaca cgcttttgtc tggctgggga ggaaggattc gaaccttcgc
     8401 atgctggaat caaaatccag tgtcttaacc gcttgacgac tccccaactt gtttggcttg
     8461 gctggggagg aaggattcga accttcgcat gctggaatca aaatccagtg tcttaaccgc
     8521 ttgacgactc cccaacaatg tttatacgga caacaaagga tgttttgaaa gaccctctgc
     8581 caaatatgcc tcgtacaaac ctgaaacttg tcggtatata ttgtatgcgc tattcctagc
     8641 ttgaaatgct gcaaatatac aggctcctga gccggtcatc attgcagaac cgtatttcga
     8701 caactcggaa taagctttcc aaacttcagg gtattcttta aataccactg cctgcatatc
     8761 gtttctgaac ggttgcaggt tttggaaagt cggcattatg cttgaggcag aatcccgtgt
     8821 caagccttcg tatgtgaaaa tttttgcggt ggaaacgtgg acgggaggtt tgacaataac
     8881 ataccactgt ttcggaatat ccattcctat cagtttttca cctatcccgc ttgcaaaagc
     8941 attttttccg aaaataaaaa acggtacgtc cgcacccaaa gccgcaccca aatcaatgag
     9001 ctgccactgc gtcaaaccac actgccacca acggttcaac accagcaaaa ccgttgcggc
     9061 atcggaactc ccccctccca agccggcgcc ggtcgggatt tttttgtcca gccatatttc
     9121 cacgccggca aggttgcgcg catatttttg cagcaacgat gcggcacggt agctcaaatc
     9181 ggcttcctgc ggtattccgc cgacaggatt gtgcaggatg attttgccgt cgtccctcgg
     9241 tttcaaatat acggtatcct gcaaatctat caggcagaat atgctttcga tattgtgata
     9301 accgtcttcc cgcctgccgg taatcctcaa atcgagattc agttttgcag gtgcgggaaa
     9361 cgcctgccgt ccgtccgcaa tattcatctg tccgccttat ctcgtgcgcg ccgcacagcg
     9421 ttccggggtt tcggtttcag acggcattcc aatttcggtg aaaaccagcc tgatgttcaa
     9481 atttccgtta ttcagttgca acgttcggac ttgcccccca ctgtcggcgg ttctgccgat
     9541 tgtccaaccg tattgttcca atatgccgtc tgaacggatg cggtaaggcg cgcccgccac
     9601 acgcctgcct tccgcccaga tatgcagata ttggattggc agtttgaaac cgaccagctg
     9661 cctgctcaag tcttccgtac cctctgcctg atagacattt cctttgccgt ccactgccaa
     9721 tgcgccgtcc ctgtcttgac acaactgtcc gagcgtgccg cccaaagggg tgttgatatt
     9781 gatggtttcc acgggcggtt ggtatgtcca atcgaaattt gcatacgaac ctttcccttc
     9841 cgctttgact gccaaccgcc cttccgctgc aaaactgctg atgtgttcgg acggctgcca
     9901 caggttttcg ttattttgag gtaattgcgc gcaagcggtc aaaagcagga tgaccgatgc
     9961 ggatacggtg tgtttcatca gagtttcctt aacgggtgtc atcctgccgg ccgacgggtg
    10021 cggcgtgccg tgcaaaacgc gtgcgaactg cgtgccgttt gcccgaatgc cccggcttcc
    10081 cggcaaacct tatgccgtct gaaaggatgg gcctgcatta tttccggggt tttcgggaag
    10141 gctcgggcaa ggcgattccg tagcgtttga gcgtctcccg ccatattttc ttgtctcccg
    10201 taaggtgtgc cgcctgcgtc catacgtcaa ccgcctgatc gcgttcgccc aatgcccaca
    10261 acacttcgcc caaatgggcg gcaacttcgg gctcggggtc gttttcaaac gaataccgca
    10321 gatacggcag cgcgctttcc gcgtcgcctt tcaggtaata cgcccagcct atgctgtcgt
    10381 taacggcggt atcgtccggg ttgatttggt atgccgtctg aagcagggcg aaaccctcgt
    10441 ccaaacgttt ggaatcggaa agcaggctgt agcccagatt attcataatt tgtgcattat
    10501 cgggcgtaag tttgagcgcg gtttcaaggt cggcaatcat ttttccccgt ttgccgaact
    10561 gttcgtaaat aatggaacgc tgtgccaatg cttccgccaa aggttccgtg cttcccgccg
    10621 ccgaaagttt ggcgatgatg ttgttcagcc cgatcagggc ttcccgtttg tcgggcagct
    10681 tcgacagggc gagcatctgt attttggaca aattgtctgc cgtaaaatac cgcccctgct
    10741 gttcgggaag tttccgcacc ctgccgatct gccgcaaagc cgcccggcct ccgtccaatt
    10801 cggcagccgc cgcagccgcc agcacgcctt tgtcgaacag gtattccggc gcggacactt
    10861 ttttcaacca ctgcctgact ttggcgtaat ccctgcggtc ggcatatatc atcgccgccg
    10921 tcattgccgc cctgccccgc tgttcccccg tccccctgcc gtatgccttt tcggcgtagc
    10981 cgtcgataac ggacgcacct tcttttcggt ttgccgccaa tatcgccgcc tgaatataca
    11041 ggtttgcatt cgggttgtgt tccaacagca cgttcaaacg cgcataggca tcatccggct
    11101 tacgcaggga aaccagattc ataatttcca tttcctgcca gacggccgaa aggttttggg
    11161 tgtctgtctg ctcgaaaaag ccgtcgagta tttcgggata tttgcgtgca gtcagacgca
    11221 acgtcattaa agtggggggc aatatttccg tatcgagctt cgccaaacgc tgcaaagctt
    11281 cgattgcctt ttccttttcg cgtccctgta cgccgaacac cgcatcggca accgccgctt
    11341 cgggcagatg ttcatacttc aacgccgcac ggcgaaccgc tttcgatgct ttttgagcca
    11401 ccccaccctg ctgcacggcg gcttgcacca gcagcaaaaa tatcctgcgt ttttgcacat
    11461 cgtccgattg cgccagcacc tcttccaacc cgtccagatg ctgatttccc ccttccctca
    11521 atacgttccg cagccacccc gcccgttttt gcgcctcacc cggtataggc tcgatctgcc
    11581 gccatttctg ataaatcatt tccgcctgtt caaacgcgtt cagcgacacg gccatttcca
    11641 aggcgcgttc ggcgacttcg ggggattttg tgcgttccaa catcagcata taggttgcca
    11701 gagccgttcc cgcctgccct ttctgcaaag ccgtttcacc gcccaacagc gtaaacaccc
    11761 tgttgacccg ttcgcccact gccgcaagcc gtgcgcgttc gtttttgatt tcttcctcgc
    11821 tgtaacgccg atgtttcctt aaaacctttc cgacttcctt cggcagctcc acatccgccg
    11881 cgccggcagc atacgcctgt ccggcaagca gggctgctgc gaggacagat aaaatagtga
    11941 aacgggcggg taacataatc atcctttatc cgacatccgt ccgaaccttc agacggcata
    12001 ttgagtatct agacaaggcg tcactttatc acaagccaaa cggtttgtag atttgcaaaa
    12061 cccgcccgaa ggcagggctg tgttttgaga ttgcgtcggt ttgcctgtat gcgatgccgg
    12121 acaatccttg ccttattatt tggtttgaca gtttaatttg ttgaacaaaa agaaatttat
    12181 ttcattccgt ctattttccg acattattcc gaagcacagc cgacggcaaa atgccgtctg
    12241 aaacgcaaat ccgcttcagg cggcattccc ggctccggcg ggctaaggca ggccgacttc
    12301 cacattgtca atcagacgcg tcgtccccag acgggcggcg gccaagacca ccagtttctt
    12361 atctcccgcc cgcgccattt cgagcgtatc ggcgcggcgg atttccacat aatcgaccag
    12421 ccagcccgca gcggtcaaat ggtcggcagc ctgccgctcc aaacccgcat aatccaaacc
    12481 gccctgcttc agcgattcgg caacagcctg cagctcgcgg tacaggcgcg gtgcttcggc
    12541 gcgttcccca acgctcaagt accggttgcg gctggagagt gccaaaccgt ccgccgcccg
    12601 ccccgtatcg acggggacaa tttcgatatc gaaattcaaa tcctcggtca aacctttaat
    12661 cacggcaagc tgctggtaat cttttttgcc gaaacaggca acatccggca aaacgatatt
    12721 gaacagcttg gacaccaccg tcgccacgcc gcgaaaatgg ccggggcgga atttgccgca
    12781 caattcgttt tgcaaatggg gcggttcgac gttgtaacgc tgttccacgt tcggatagag
    12841 ttctttctca tcgggcgcga aaacaacggc aacgccttcg gcggcaagtt tgtccgcatc
    12901 ctgttgcaaa gtacgcggat atttgtcgaa atcctcgccc tgaccgaatt gcaagcggtt
    12961 gacgaatatg ctgacaacaa cgttgtcggc gcgttttctg gcttcgcgaa caagggcaag
    13021 atgcccttca tgcagattgc ccatggtcgg cacaaatgcc acctttccta tattttcacg
    13081 ccacgtgcgc agttctcgaa tggtatgtat gatttgcatt acggtaatcc ttatccttcg
    13141 ggcttcctgc ccgacaaaac gcgccgatta tacgcgccgc gcacggcaaa acaaaatgcc
    13201 gtctgaaacg gctttcagac ggcatcggca gcagaccttg ttcaatctgc aaaaatatgt
    13261 tccgcagcag ggaaggtttt ggctttgact tcggcaacat acgcccgaac cgcagcctgg
    13321 atgctgcttt gcccgcgcat aaagtttttg acgaatttcg ccgttttgcc cgggaaaata
    13381 ccgagcatat cgtgcatcac caaaacctga ccgtcgcaat ccgcgcccgc cccgatgccg
    13441 atggtcggac aggaaacagt ttcagttacc ttttttgcca gttccgccgg cacgcattcc
    13501 atcagtacga cggcagcccc ggcttcatca tgcaccttgg catcgttaag caacgcctgc
    13561 gccttgccgc cgcgcccctg aactttatat ccgccgaagg caaacacgga ttgcggggtc
    13621 agaccgatgt gggcacagac cggaattccg cgcatttgca gaaattcagt cgtttccgcc
    13681 atccacacgc cgccttcgag tttgaccata tgcgcgccgg cagccatcag ttcggcggcg
    13741 gcggcaaacg cctgctcctt actctgctga tatgcaccaa acggcaaatc gctgacaatc
    13801 atcgcatttt ttgcaccgcg tgctacacat tcggtgtgat agcacatatc gcgcaggctg
    13861 accggcagcg tcgactgtcg cccctgaacc gccattccca aagaatcccc gaccaacagc
    13921 acatccacgc cggcatcgtc catcagcgcg gcaaaactgg attcgtaagc ggtcagcata
    13981 acgattttct cgccggccgc cttcattttt tgcagtgtgt tcacagtaat catcagatat
    14041 tgttaccccg cccttttcag acggcctttt cagtttgaaa cgggctatta tagtgaaatg
    14101 cccgcagccc tgccaccgac cgtgccgcaa atgcaaaaca gcccgactgt tttacacaat
    14161 cgggctgtca aatctggtgc cggcaccaag agtcgaactc gggaccccct gattacaagt
    14221 caggtgctct accaactgag ctataccggc aaagaaggcg aattatgcag gcaacttcac
    14281 actttggcaa gaaatttgtt cgaccccctc ataaaaatac actaagtatc ttatttaaaa
    14341 caaaattaat ttgaaacaga acaagcatcg gcattctgat aaaatatccg cctttcaaac
    14401 cgaccgttca caccgatggc aagacatccc taccgccgcc tgcgtcctgc aaaatccggc
    14461 tttcccgaag tcggtatttc cgaagaaggc aatatccgtt cgcttcactt gggcagcgac
    14521 accgttcaaa gctcgatgaa cctcgaccgc ccgtccgagc tggtgctctc ttacagccgc
    14581 gcgatgatgg gctggctgct gtttgcggaa cgcctgccgc aacatatcac ccaaatcggc
    14641 ttgggcggcg gttcgtttgc acgctggaca gacacctacc tgcccgacac gcgccaaacc
    14701 gccgtggaca tcaatcctca ggtcatcgcc attgcccgca acctgtttga gttgcctttc
    14761 gagggcggaa aatttgaaat tatcgaagca gacggtgcgg aatacatcaa agtcttccgc
    14821 cacaacaccg atatcatctt ggtggacgga ttcgacggcg aacaaatcat cgatacgctg
    14881 gttgaagaac cgtttttcag agactgccgc aacgcactct cttcagacgg catattcgtt
    14941 accaactggt ggagcggcga caaacgctat caacgcttca tcgaacggtt gttgagcgtt
    15001 tttgaagggc gcgtattgga acttcctgcc gaaagccacg gcaatgtcgc ggtaatggcc
    15061 ttccaaagca gccccaaaga gcaaaacata gacaaactca aaaaacgtgc cgacaaactg
    15121 agcaacgcat acggattgga cttccaccgc atgctcgccg gcctgaaagc gtccaacccc
    15181 aacaacggca agcatttcca cctttaagcc gccatcttcg cggctgcatc ggaatatgcg
    15241 gaaaatcaag aggaaaccat tatgctgaaa cccgaaacca tacacatacc cggccctgcc
    15301 ggcattttag aaaccatcca tatcccgtcc gaacaagtac cggcacgcgg tgttgccgtc
    15361 atcaaccacc ccaaccccct ccaaggcggg acgaacacca acaaagtcat ccaaaccgcc
    15421 gccaaagcat tgagcaagct cggcttccac tgctaccttc ccaaccttcg cggcgtaggc
    15481 ggcagcgaag gcacacatga ttacggacgc ggcgaaacgc aagactgcct cgccgtcatc
    15541 gactatgccc gcgcccaaca tcccgaagcc ccggaatttg ccttatccgg cttttccttc
    15601 ggcggctatg tcgccacatt tgccgcacaa gtgcgcatac ccgatttatt gctgctcatc
    15661 ggtgcggcag tctgccacta taccgaccgc cccgagccgt ccgccgttcc caacgttgcc
    15721 aaaacgctga tgatacacgg cgcggaagat gaggtcgtcg aaatcgaaaa agccctgaaa
    15781 tgggcagaac cgcaagattt gcccgtcatt accatcgccg gttcgacgca cttctttcac
    15841 ggcaaactca tcgtcctgcg cgacaccatc ctccgcttcg cacccgtatg cctgaacgga
    15901 taaagcccct ttcagacagc atcgataata tcaaacccgt atccggcggc atctgccgat
    15961 acgggtttgc gttccctctc attacaggca aaacccgctg caaaacaccc tttcccgacc
    16021 cgacaaagga tctgcaaacg caatcttttt tgccaaaagc ttcaaaggtt tccgataatc
    16081 ctcgctaccc gcttcagacg gcacgggata gagcgcgtca ttcaacagcg gcatacccaa
    16141 acccatcata tgcacgcgta actggtgttt cttgccggta tgcggcgtaa ggcggtaaag
    16201 gctgaattcc cccctgtttt caatcaattc gaccgtcgtg tgcgcattcg gcacgccttc
    16261 ggcctcttgc gtcgtaaaaa atttctcacc ccttaccaaa cgcgaaacca catcgagcgg
    16321 atacggcaaa tccgtctttg tcggcgcaag cgcctcatac gttttccata ccgttttgtt
    16381 ttgaaacatc gtctgataag ccccgcgtgt ggcaggattg tgcgacagca gcatcacacc
    16441 cgccgtatcc ttgtccaagc ggtgaatcgg cgtaatgtct tcaacattca aatgctgcaa
    16501 ttcgggacgc aggcgcagac gcgtgagcag cgtttcccgc aaaaaacgcc cgccggggat
    16561 gacgggcaga aaatgcggct tgtccaccac aatcaaatgt tcatcgacaa acaaaatctt
    16621 ttcttcaaac ggaatacgcg gctcgctctc acaaccggtt tcacggtaat aaaacatcgt
    16681 cttaccgggc tcgaacaaag aatgttcgtc caacgccgca ccatccgaac cgaccacaaa
    16741 accgctgttc aaccgcctcc gccaatcgtc cgctcccaaa aaaggaaagc ggatgcacag
    16801 aaaatgcagc agcggcagcc cgtaaaactg cttttcatgc ggcagcacca aataactggg
    16861 tttgacaccg ttcaacagcg gaagaggatt attgcgtttt ttcatgcggc ggattgtaac
    16921 cgtttttcac aaacttttc
//
LOCUS       DAWTHD010000045        16627 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWTHD010000045 DAWTHD010000000
VERSION     DAWTHD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.045"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(318..1034)
                     /gene="trmB"
                     /locus_tag="WM704_001117"
     CDS             complement(318..1034)
                     /gene="trmB"
                     /locus_tag="WM704_001117"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFA9340548.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGSYIHLATDWEEYAQQMLEVLSSFDNLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1156..2561)
                     /locus_tag="WM704_001118"
                     /pseudo
     CDS             complement(1156..2561)
                     /locus_tag="WM704_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(2678..4564)
                     /gene="uvrC"
                     /locus_tag="WM704_001119"
     CDS             complement(2678..4564)
                     /gene="uvrC"
                     /locus_tag="WM704_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFA9340549.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSDQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(4619..6796)
                     /locus_tag="WM704_001120"
     CDS             complement(4619..6796)
                     /locus_tag="WM704_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HFA9340550.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            6935..7885
                     /gene="trxB"
                     /locus_tag="WM704_001121"
     CDS             6935..7885
                     /gene="trxB"
                     /locus_tag="WM704_001121"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFA9340551.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8041..8409
                     /gene="rpsF"
                     /locus_tag="WM704_001122"
     CDS             8041..8409
                     /gene="rpsF"
                     /locus_tag="WM704_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFA9340552.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            8410..8712
                     /gene="priB"
                     /locus_tag="WM704_001123"
     CDS             8410..8712
                     /gene="priB"
                     /locus_tag="WM704_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFA9340553.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            8719..8949
                     /locus_tag="WM704_001124"
     CDS             8719..8949
                     /locus_tag="WM704_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFA9340554.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            8966..9418
                     /locus_tag="WM704_001125"
     CDS             8966..9418
                     /locus_tag="WM704_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFA9340555.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(10041..10847)
                     /locus_tag="WM704_001126"
     CDS             complement(10041..10847)
                     /locus_tag="WM704_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFA9340556.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(10848..11594)
                     /gene="pssA"
                     /locus_tag="WM704_001127"
     CDS             complement(10848..11594)
                     /gene="pssA"
                     /locus_tag="WM704_001127"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFA9340557.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12177..12659
                     /locus_tag="WM704_001128"
     CDS             12177..12659
                     /locus_tag="WM704_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFA9340558.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(12706..13917)
                     /locus_tag="WM704_001129"
     CDS             complement(12706..13917)
                     /locus_tag="WM704_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HFA9340559.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            14139..16577
                     /locus_tag="WM704_001130"
     CDS             14139..16577
                     /locus_tag="WM704_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HFA9340560.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         3796 a         4163 c         4708 g         3960 t
ORIGIN      
        1 tttccagtaa atagaagttt agttgttttg ttaactatgc ctgaatgtac acctaagtct
       61 tcttttggta acgatgaata attagcaagc cgtagcctgc atctccgcca accgcgtgcc
      121 actatctgcg ggcggaataa actttaaaac cgatgtgcag gttaaaacct acaaggatgc
      181 gtgcggtata cacgcgtttt tgccgttcaa accattgttt caaaacttgt attgaaacat
      241 cgcccgctgc tttatcttga aagaatgccg tctgtaagca aacatgcttc agacggcatt
      301 ttttatccgg cggtctgtta tccgatccgt ttgaatacca agtcccaaac gccgtgtccg
      361 aggcgtttgc cgcgcgcttc gaatttggtt tcggggcggt agtccggagt gggggcgtag
      421 tctgccgccg tattttgcag gttgtcgaag ctgctgagga cttcaagcat ctgctgtgca
      481 tattcttccc agtctgtcgc caggtggata tagctgccgg ttttgagttt gggcagtagt
      541 ttggcgatga agggggcttg tatcagacgg cgtttgtggt ggcgtttttt gtgccacggg
      601 tcggggaaga atatgtggat gccgtcgagc gagccgtctt gcagcatatt ttcgacaact
      661 tctacggcat cgtgccgcat cacgcggatg ttttccaaat ggttttcgtt gatgagtttg
      721 agcaggttgc ctacgccggg gccgtgtacg tcgatggcga gaaagtcggt ttcgggcagg
      781 cggcgggcga tttctgcgct tgccattccc ataccgaagc ctatttcgag gattttaggt
      841 cggctgcttc cgaaacgggc atcaagatcg gccggtgttg cttggtagtc gatgccgaag
      901 ctgtcccata aggtatcgat ggcgcgttgc tgtgcggcgg tcatgtggct ttggcggagg
      961 acgaaactgc ggatgctgcg ggggtgtccg gcttgacggt cgtttggtgt ttggttttcg
     1021 gcggtgtctg tcataatgct tctgtgcgct tgtgcaatca ggatgccgtc tgaatgtgaa
     1081 aggcctgttc ggacggcggg aagggaaagg ggcgattata cctgtttcgt ttcggcaggg
     1141 taggtgtaac tctgttcaga cggcattttg ccactgttgc agcagttctt gcaggacgct
     1201 tttttgcccg tatccgttgt ttatggcttc ttgcaggtag cggcaggctt tttctttgtc
     1261 cggcctggca ccgtgtccgc tgtaatggat gcaggcgagg ttgtagaacg ccatactgtc
     1321 tttttgcgcg gcggcttgtt caaaccattt gcgggctttg cagtagtcgg cggctatgcc
     1381 ctgtccgaag tagtacatac tgcccagatt ggtttgggct ttgctgtgtc cgagttctgc
     1441 tgcttctgtg tagagtttga gcgcggctgc gtattcgggc gcgcattgta atccgtaatg
     1501 cagggcttgg gctttttgat aaagctgttc tgccctttgc cgcctaatgg cggaatcttt
     1561 gatgccgtca tattgctcgg gatggtttaa cgcgctgtcg gatatgagtt tctgataggc
     1621 ggtaaggctg ccggcttcgg ctgcctgccg gtaataatgc cgggcttttt ccgtatcgag
     1681 ggctatgccc aaaccgtagc ggcagatgtc gcccggtatc cgcaaacctt cgggatggcg
     1741 ttcggcggcg gctgcttcgg catgatgcag ggcaaatttg tggtttcttt ccagatactt
     1801 gccttgcagg tagatgtcgg ccagtgcggc atgggcatcg gggtcatttt cctgtgccgc
     1861 ctgttggaac cggtaaacgg cttcatcggg agccgtaggt aggatgagtt cggcaagcgg
     1921 ggtgtaggcg ggaatgtatc cttgttgcgc ggcggcgcgc aaatgattga cggcctgtgc
     1981 cgtgtcggtt ttcgtccctt gtccgaaacg gtattgcagc ccgagttgcc aatgtgcttc
     2041 cggcaagcct tgtgccgccg ctttgcggta gagcaggtgc gccgtttcaa aatccggcgg
     2101 cgtgccgtgc cggcagtatt gggcgagcag gtattgcccg tggggattgt tgttttttgc
     2161 gcttgttttg cccattccag ggcttcttca aaatgtcggg acgcgtacag aaggtacatc
     2221 aggcggactt gcgcttcggg caggcctgct tctgccagag gcctgtaaag cggtatcagg
     2281 gtttccgggg gcgcgccttc caattcttgg atatatgcca aattgtaacg cgcataggga
     2341 tgcccctgtt cggcggcgat gcggagcatt tccatacctt gttcgcggtc ttggtgcggg
     2401 ctgtccgcgt tcatcaggtg ttttgccaac ttgaatgcgg cggcgggatg atctgccgag
     2461 gccgcttctg tcagcagtcg gcggacttcg gtaaagttat aatattgttt ttgagacagg
     2521 cgttccaagg cctgccgata gggtaagtcg ggattgttca tatcagatcg ttttccgttt
     2581 tgtcgaatca tcgttattcg tcatgaagtt aggtttatat cggtatgccg gcctgctgta
     2641 tcatattttt acaggcggtt tttgctttgg cggcatacta atgcagatga ttataaatgg
     2701 tttccgccaa tgccttgctg atgccttcca ctttttccaa gtcctcgcgg ctggcggcaa
     2761 tcacgccgcg cagtccgccg aagcgggtaa gcagggcttg gcggcgtttg ctgcctacac
     2821 cgggaatgtc gccgagagac gaggtaacgc gtgctttgtc gcgtttttta cggtggcctg
     2881 tgatggcaaa gcggtgtgat tcatcgcgta cggtttgcaa taaatgcaag gccgggctat
     2941 taggaggcag gcggaagagt tcgccggtaa aaggcagtat gagttcttcc atgccggctt
     3001 tgcgttcggg gcctttggca ataccgacta aagggatgtg cagcccgagt tcttcccata
     3061 cggatacggc tacgccgatt tgccctttac cgccgtcaat caacacgaca tccggccatt
     3121 tgacgctttc gccgttggct tcggcttcct gtattttgcc ataacggcgc gtcaacactt
     3181 cgcgcatggc ggcgtagtcg tcgccgggtt tggcggtggt gatgttgtag cggcggtatt
     3241 gcgagggttg gatgttttgt tcgtcgtaca cgacgcagga ggcgatggtg gcttcgcctt
     3301 gcgtgtggct gatatcgaaa cattcaaggc ggttgattcc gtctgaattc atgccgagga
     3361 ttttggcaag ttcgtcgatg cggtgttgtt ggttgctttg ttgcaggtgg cgttgggtaa
     3421 tcgccatttg tgcgttttgt tccgccattt tcaaccaaac tttgcgttcg cctatggttt
     3481 tggtaacgaa ctgcatctgc ttgccgtgtt ctccttccaa agcctctttc aacgcatcgg
     3541 gaacgggaaa gttgctgatg atgatgtcgg gtttgctttt gcccagatag tgttgggcga
     3601 cgaaggcttc ggcgtaatct tgaccgtttg gttcggggtc gtttttggtg tcgggaaaga
     3661 agcttttgtc gccgacgtgc cgtccgccgc ggatgctgac ccagtgtacg cagacaaggc
     3721 cgtctgaaac ggcgagcgcg agcaggtcga tatcgttggg gttgttcggg tttttgctgt
     3781 cgatgaactg gtcgctctgt ataatgccga gtgcttggat ttggtcgcga taacgggcgg
     3841 cttcttcaaa ttgcagattc gcggcggcgg tctgcatttt gtgttgcagg gtgcgggtca
     3901 gttcgtcggt tttgccgttg aggaaagtgg cggcttgacg cacgctgtcg cagtagtctt
     3961 cttcgctgat gtgtccgacg cagggcgcgg tgcagcgttt gatttggtac agtaggcaag
     4021 ggcggtcgcg gtgttcgaac acgctgtctt cgcaggtacg cagcatgaag actttttgca
     4081 acacttgaat gctgtcgcgc acggcgttgc tgttcggata cgggccgaag tattggttgg
     4141 gatttttcag cgtgccgcgg taatacgcca tttgcggata ttgatggccg ctgagcatca
     4201 aataaggata gcttttgtcg tcgcgaaaaa ggatattgta tttcggcgac aaggctttga
     4261 tgaagttgtt ttcgagaatc agggcttcgg cttcggaacg cgtgatggtg gtttcgatgt
     4321 ggcgaacctg tttcaccatc aacgcgatgc gcggcgaatg gtcgtttttt tggaaatagc
     4381 cggacacgcg ccgcttgagg ttgaccgctt tgccgacgta taagacgttg ccgccttcgt
     4441 caaaaaaacg gtacacgccc ggcagcttgg gcaggttttt gaggaagagc gggatgtcga
     4501 attgttccgg agaacggttt tctgtattca tatcaatcat ttgaaaaacg gcttcagacg
     4561 gcatatcggc agcggcagcc ttatccgttc ggaaaagctg tcgggcggtt atatgcggtc
     4621 aatcgatttt tacccatttc aggcgcaggg cattgcccaa taccgaaacc gagcttgccg
     4681 ccattgccgc gcctgctatg acgggattta aaaagccgag cgcggcgagc ggaatgccca
     4741 atatattgta gaagaaggcg aaaaataggt tttgcttgat gttttccaac gttgcctgcg
     4801 atatcagcag ggcatcggcg agctgattga ccgaatgctg catcagcgtg gcggaggcgg
     4861 tgtgttcggc aacgtccgca ccgcctttca tggcgaagct gacgttggcg gcggcaagcg
     4921 cgggcgcgtc gttgatgccg tcgccgacca tcgccacggt tttgccggcg gctttgagtt
     4981 tctgcacttc ggcggctttg tcgcacggac tcatattacc gaaggcgtgt gcgatgccca
     5041 gttgtttggc gacgtattcg accgtacttt ggttatcgcc gctcataata tagacatcaa
     5101 tattgtgttt tttcagacgg cctatggctt cggcggtatc ggctttcaac gcgtcggaga
     5161 gtgcgaatgc gccgatcggt ttgccgttta cagatacggt aaccgcactg gcgatttccc
     5221 aaacgccgtc tgaaaacttc ggcaaggtca gttcggcaaa ttcggctttg cctgatttta
     5281 ccaaacccac gccttccact tcggcggtaa tgcctgctcc gacaacggtt tgcgcatttt
     5341 gtgcagcggg aatctccaaa ccgcgcgctt gtgcggcgga gacgatggcg cgggcgagcg
     5401 ggtgggcggc gttttgctcg acggcggcgg cgatgcggta caaagcgtct tcgtcaaagc
     5461 cgctgtcggg aacgtaataa acggcggcaa cctgcggcct gccttcggtc agcgtaccgg
     5521 ttttgtccaa tacgacggca tcgacgtggg ctgcttcctc cattgccgcc gcgtctttaa
     5581 accaaatgcc gtgtttcacc gctttgccca tgccgaccat aatcgcggca ggggtcgcca
     5641 gaccgagcgc gcacgggcag gcaatcacca aaacggcaac ggcgtgcatc agtgcgaccg
     5701 tccaatcgcc cttaatcagc caagtaacga taaaagtcag aagcgcgatg cccacgacag
     5761 ttggcacaaa taccgccgcc gctttatcgg ccacgcgcgc aatcggtgct ttactgcctt
     5821 gtgcttcaga gagcgcgttc atcatgtcgc cgagcagggt ttggctgccg agctgcgcgg
     5881 cgcggtacac cacgctgcct tcggtcatca gcgcgcccgc caacactttg ccgcccgcct
     5941 ttttctcttc gggattggat tcgccggtaa ggtggctttc gtccgcccaa ccgctgccgc
     6001 tttcgataat gccgtcggca gcgatgcgtt cgccgtggtt ggtgcggata aggtcgccga
     6061 tttgcacttg gtcgatgggc agttgtttcc attcgccgtt gcgttgcacg ttgacttggg
     6121 tcggcgtgag ttttagcagt aagcccaagc tgttcaggct ggattttttg gtgcggtgtt
     6181 ccaaaaactt acccagcgac acaaaaccga tcaccatcac gcccgcttca aaatacacat
     6241 gcgccatacc gtgcgccgca tgcgaactga aaaacagcat ataaacggaa tacaggtaaa
     6301 tcgacaccgt gccgatggta acgagtacgt ccatattcgc cagcccgcct ttaatgcttg
     6361 cccacgcgct tttgtaaaac gggattgcca gccaaagttg cactatgctt gccagtacaa
     6421 actgccatac aggcggaatc atccaatcgt gccgtgtcca attcagccct tttagcatca
     6481 tccctaccat accgataagg aacgggatat tgatggtcag caaaagccac aaccgccagc
     6541 cgatatggtg ttctgcttca ggttgcggca atgtatcttc cgttttttcc ttcgcgccgt
     6601 aaccggtttt ctcaatgatt ttggcaatgt cggcaaccga ggttttgctg ccgtcaaacg
     6661 taacctgcgc ttcctcactg gcaaagttca ctcccgccga ttcgacaaaa tcttttttgt
     6721 tcaacacttt ttcaatgcgc gaagcacatg cctgacaggt catcccctcg atttggaaac
     6781 ggattttttg ttgcataagt ttgattcccg ttttgatgtt cagacggcag tcaaggctgc
     6841 gggatgccgt ctgaacgccg gtatggtcaa aatttctcaa acgcgccgat aattcgggta
     6901 gaatccgaaa acttgacaca gcaaaggaaa aacaatgtcc caacaccgca aactgattat
     6961 tttgggttcc ggccccgccg gatacaccgc cgccgtctat gccgcacgtg ccaatttaaa
     7021 ccccgtcatt attacaggta tcgcgcaagg cgggcaactg atgacaacga ctgaagtgga
     7081 caactggcct gccgatgccg acggcgtgca agggccggaa ttgatggcgc ggtttctcgc
     7141 ccacgccgaa cgtttcggaa cggaaatcat ttttgaccaa atcaacgccg tcgatctgca
     7201 aaaacgcccg ttcgcactca aaggcgatat gggcgagtac acttgcgatg ccctgattgt
     7261 cgccaccggc gcgtccgcca aataccttgg tttgccgagt gaggaagcgt ttgcagggaa
     7321 aggcgtttcc gcctgcgcca cctgcgacgg tttcttctac aaaaaccaag atgttgccgt
     7381 agtcggcggc ggcaatacgg cagttgagga ggcactctac cttgccaata tcgccaaaac
     7441 cgttacgctg attcaccgcc gcagcgagtt ccgtgctgaa aaaatcatga ttgacaaact
     7501 gatgaaacgc gtggaagagg gcaaaatcat cctcaagctg gaaagcaacc tgcaagaagt
     7561 actgggcgac gatcggggcg taaatggcgc attattaaaa aacaacgacg gttctgatca
     7621 acaaattgcc gtcagcggca tctttatcgc catcgggcac aagccgaata ccgatatttt
     7681 caaagggcag ttggaaatgg acgaagccgg ctacctgaaa acaaaaggcg gcacggcgga
     7741 caatgtcggt gcaaccaata tcgaaggcgt atgggcggcg ggcgacgtaa aagaccatac
     7801 ctaccgtcag gcaattacca gcgcggcctc cggctgtcag gcggctttgg acgcggaacg
     7861 ttggctgggc agccaaaaca tttgaatgcc gtctgaaacc gaaattttcg gcggaatgca
     7921 agtttttcgt accaaatcct tgattccgtc ggcaaacccc aatataatgc ctgctctttg
     7981 aaccttgcct tctgctttcg catggcagaa ggctgttttt accatccata aggagataac
     8041 atgcgtcatt acgagatcgt gtttatcgtt catcctgatc aaagcgagca agtgcccgct
     8101 atggttgaac gttacaaaac catgattacc gaggcaaacg gtaagattca ccgtttggaa
     8161 gattggggcc gccgccaact ggcttacccg attaacaaaa tccataaagc acattatgtt
     8221 ttgatgaaca tcgaaaccac tcccgaagtt gttggggagt tggaaaccgc gttccgtttc
     8281 aatgatgcgg tattgcgtca tctgaccatc aaaaccaaac acgccgttac cgaagcctcc
     8341 cctatgctgg gcggcgaaaa ggctaagaac ctgttggtcg gcgcggctga agaagcggct
     8401 gcccaataat tgggattcac taatcttgtt tcgcttgccg cgctgattga aaaggctttc
     8461 cctattcgat atacgcctgc cggaatccct gttttagata ttattttaaa gcacgaatcg
     8521 tggcaggagg aaaatgggca gcaatgcctt gtccaattgg aaatccccgc gcggattttg
     8581 ggtaggcagg cggaagagtg gcagtatcgg caaggtgtat atgttcacgt cgaaggtttt
     8641 ttagctcaaa aaagcagacg ttccctgatg ccgatgctca ggatacaaaa tattaaagaa
     8701 tataaaggtt aaacgacaat ggctcgtcaa tcattcaaac gtagaaaatt ctgccgtttc
     8761 acggctgaaa aaatccaaga agtcgattac aaacaagttg atttgctgaa agactttatc
     8821 tctgaaaacg gtaaaatcat tcctgcccgc atcacaggaa cgaaggcatt ctaccaacgc
     8881 caattggctg ttgccgtaaa acgcgcgcgc ttcttggcac tcctgcctta taccgaccaa
     8941 cacaaataat tttggagttt aaatcatgca aattattctg ttagaaaaaa tcggcggttt
     9001 gggtaatttg ggcgacatcg ttaccgtaaa aaacggttat gcccgcaact tcttgattcc
     9061 tgctggtaag gcaaaacgtg ctactgaagc aaacatgaaa gagtttgaag cacgccgcgc
     9121 cgaattggaa gccaaacagg ccgaaatttt ggcagatgcc cgagcccgtc aggaaaaact
     9181 ggacggtcaa accgttaccg ttgcacagaa agccggtgtg gacggccgcc tgttcggttc
     9241 cgttaccaat gccgacattg ctgctgcaat cgttgctgcc ggcatcgaag ccgtgaaagc
     9301 aaatgtacgt ctgccgaacg gtcctctgaa agccgttgga gagtacgaag tggaagtggc
     9361 tttacacact gatgccgttg ctaaaattac tgttgccgtc attgccgcag ccgagtaatt
     9421 gaaattatca gatgccgtct gaaaccctgc cgattatcag aatgtggttt cagacggcat
     9481 ttgttttgtt ttccgtatat tctgcaaaaa tcgggacggt tgttttttat ttgcctgagg
     9541 tctttgcaga aatggtttgt taacgaaatt tgatgcataa aatgcgccaa aaaatattgg
     9601 ctgtgtttta atatagtgtt gacaagcgta cctggtttca atatattcaa taggataggt
     9661 ctcttcccaa ccgaaaaaat aattgtcttt tctcttgtaa atagttgaaa tagattgtca
     9721 aaatacatcc taaaaaattt aataacttat atgattgcaa aggttgcggc ttgtatgccg
     9781 gtcattatgg cggaaatacc gtttgggagg cagtcgcatc gcatggagtc ggcacggcag
     9841 gcgatgcgcc gatgccttcg ggcgtgaagg cagggaaatc ggcagtttgg aaaaaggcaa
     9901 tggaatccgc aggtaggtgt cgattgggtg gtgaaaggca ataagtcggg cgcacgctca
     9961 gactgcctat ccccttgtcg gaacgggagg tagggcagct ttgtcctgac taattgcttg
    10021 tgcaaacggt aagaaggttt ttaaagcagg ttgtacagca tttttccggc aatcaaaagc
    10081 aacataatgc cgaaggattc tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca
    10141 ccgagcgggg caaaggcaat agttgccgcg ctgaggacgg cgacggcggg caggtaaagg
    10201 aagcccagcg acccttcggg caatcctgca atattcagac cgttgaccag atacgatatt
    10261 gcgccggaga gtgcaatcgg ccaggcaagg ccggatgatg tgccgatggc tttatgggcg
    10321 gggaagccgc agtggattaa gaaggggacg gaaagtgaac cgccgcctat gccgacccag
    10381 ctcgacattg cgccgaacag tgtggaaacc gcagtcagtc cgggcagccc gggcagcggg
    10441 cgggatgccg tctgacgacc ggtatgcagt gttttgaatg cgacggcggt taaaaacagg
    10501 atgaagaaaa tttgaagccc gaacgcgggg atatattttg cggagagtgc gccagcgaat
    10561 acgccgaata tcatacccgg catcatcgca aatatggttt tccagtcgac cgtctgtttt
    10621 ttgtgctgcc ccaacatact ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg
    10681 acggcgaggt gttgcgcgta aggatgttgt gccaaaccct gcaaatcaag cacccataaa
    10741 acgacaggga caatcagcgt accgccgcct acaccgaaca ggccggcaat aaaacctgcc
    10801 gcactgccta cggcaagcag gattaagata atgtcccaat gccacattta atccgccttt
    10861 ctgtattttt tccaaaatcg gcgtgccgcc ataatgtagc cggacaggct gtatccgaga
    10921 aagaacagga aaaggacgag cgacggttcc caagtgacca gaagcagcag taagacggca
    10981 agcagcattc cgacaaaggg gacttgtctg cggatgttga tttctttaaa actccaaaaa
    11041 gggatttgga caatcatcga caggccggca aacagtgtga tgcctaatgc ccaccagtgg
    11101 acggcgggga atttttcgac gctgtggttg acccaaatca gcccgacaat cagcgcggcg
    11161 gcagtcggac tgggtacgcc gataaaccag cgtttgtcca ccttgccgat gagcgtgttg
    11221 aacagcgcga ggcgtagggc ggcgcaggcg cagtagatga aggcgacgga ataaccgatt
    11281 ttgccgaact gccaaagctg ccatttgtag gcaataagcg cgggggcaac gccgaagctg
    11341 accatgtcgg caaggctgtc gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc
    11401 gccacgcgcc cgtccatacc gtccagcagc atagagatga ataccgcgat ggcggcggtt
    11461 tcataacgtc cgtgcatgga ttgggtaatg gcgtaaaacg cggaaaacag cgcggcgatg
    11521 gtaaaggaat tgggcagcag gtagatactg ttttgacgca gggagcgttg cggggtgttg
    11581 gtcggggttt ccatagctga agtgtctcac ggacgtggag aatcgatagg ggattataac
    11641 ccaaaccatt atcgaacaat acgcccttta ggatgaagcc ctcggaggcg gtttgttttt
    11701 ctgatggcgg atgctttgcg gggcggtttg actctgcctg ccaccgggtg tgttgtttcg
    11761 gattttgccg gatggatatt gttgccgggc atttctgcgc ttgccggaag gtatcggagg
    11821 gaaggcatcg gaatatgcgg cgcgccgttc aaaccggggc ggtatttccc tattttttgg
    11881 ggggtgcagg tttcaacaaa ttgaaagata ttttttgcat ttccgaccgt atgctgattt
    11941 tgaagcggga taaattatag gacaaggttt ttcgcccgcc gcaaaggttg cccgcaatgc
    12001 cgtctgaaaa caacggcttg ccgctaaggc ggcaatatga ggatttgcgc tgccgaaacc
    12061 ggtttgctac aacccggcag tgaaatctgc agggagggat aggccgataa ggcgggactt
    12121 atcttgtttt ttgtttataa tgcaacagaa tttggaggaa tcgtcgggaa tttatcatga
    12181 tgaaaaagat actggcagta tcggcactat gcctgatgac tgcggcggca caggctgccg
    12241 atacttacgg ctatctcgcc gtttggcaga atccgcagga tgcaaacgat gttttgcagg
    12301 ttaaaaccac aaaagaagat tcggcgaaaa gcgaagcgtt tgccgagttg gaagcctttt
    12361 gcaaaggtca ggacacgctt gcgggcattg ccgaagacga gccgaccgga tgccggtcgg
    12421 tcgtgtcgct gaacaatacc tgtgtcgcgc tggcataccc gaaagccttg ggcgcgatgc
    12481 gcgttgaaaa cgccgtcgtg attacttctc cgcgttttac gagcgttcat caggtcgcac
    12541 tcaaccagtg cataaaaaaa tacggcgcac agggacaatg cggcttggaa acagtgtatt
    12601 gcacgtcatc ttcttattac ggcggggctg ttcgctcctt aatccaacac ctgaaataaa
    12661 aaatgccgtc tgaaggatgt tcggacggca tttccatatc gacggtcagg attctttcgg
    12721 atcgggcagc aggctgttca acacaatggc gagtacggcg cacaagccca cgccgacaaa
    12781 gctgaagctg cccaatttga gtgtcatacc gccgatgccc gtggtcagta ccgagctgac
    12841 gatgaccagg tttttcggca gcatcaaatc gactttggca tcaatcaggg ttttgatgcc
    12901 taaagaagca atcgtgccga acagcagcag cataatgccg cccatcacgg gcatcggaat
    12961 ggaagccaaa aacgcattga atttgccgaa aaacgccatg cagacggcaa aaaccgccgc
    13021 ccaagtcatg atgacggggt tgctgttttt ggtaatcatc accgcacccg ttacttcgcc
    13081 gtaggttgta accggcgggc cgccgatcag acctgcaacg cacacgccca agccgtcgcc
    13141 cgcaagggtt ttgtccaagc ccgggtcttt tgtgtagtct tttcccgtta cattgccgat
    13201 tgccatgatg ccgccgatgt gttcgatggc gggggcgacg gcaacgggca gcataaacag
    13261 tgccgcctgc cagttgacct gaggcgtttc aaaatgggga acggcgaacc agggcgcgtg
    13321 tgcaatgctt gtcgtgtcca ccagtcccat cagcagtgcc aaaacataac ccgaagcgac
    13381 accgatcaag atgggaatca gcttcatcat cctgctgccg aaaaccgata cgatggcggt
    13441 aacggcaaag gtaaagccgg aaagaatcag cgaatcggta tagtcgatga cctgtttgcc
    13501 gtcagcctga cccattgcca tgctgcttgc cgccacggca acagacagac cgatgaccat
    13561 gatgacaggg ccggtgacga ccggcggcag cagtttgtgt accgccgcca gtccgcgcca
    13621 acggatcagc gcggcaaaca caaaatacat aaagccggcg gcaaacagcc cgaacatggt
    13681 ggaaggcagc ccccattcgc cgacggagta gataatcggc gcgataaagg caaacgaaga
    13741 accgagaaaa atcggtactt tgcgtttggt tgtgatttgg aacagcagcg ttcccaagcc
    13801 tgcgcccaaa agcgcaagag ccggattcag accggtcagc aggggaacca gcaccattgc
    13861 gccgaatgcc acaaataaaa tctgcgcgcc ggaaacggca agtttcagtt ggttcatacc
    13921 atcttctttc aatcggttca aacggcacgg attatactgg tttgccgagg ggctgcgtaa
    13981 aaatacatac gtcgggcaaa gatatgccgt ctgaaaggca acgtctgtct gttgcaggat
    14041 gaaagcggga gaaatggagt ataatccgac actttccata ttcaatccgc aagccgtccc
    14101 gaacccttat ttcagacggc attttccggt aatacggcat gacagaaaaa tcccataaaa
    14161 aaacagcaaa aggcagggcg ggaagcccgt ccccaacgtc cgcacgcaac aagaaagccg
    14221 acaacggcgc gcggggcaat aaagtttccg agcggctcaa ggcggtcaaa gagctgcaga
    14281 aaactgaagc caaaaaggcg cgtcccgaac atgtcgtcaa cctgattgcc gacgcgctgt
    14341 ggctgatggg tttggcggca accctgtatt tggcgatttc cctgatcagt ttcgatatgg
    14401 gcgatccgtc ttggtcgcac agttcaccgg ttgtggaaga tgccgccaat tggggcggac
    14461 tgttcggcgc gtatgttgcc gatgtcggct attatctttt cggctggtcg ttctggtggt
    14521 ggatagcggc tgcctgcgtc gtgctgtata aaaatttccg tctgcacgca aaacagacgg
    14581 aaaacgaggc atacaaccac aaaatcgctg ccgccgcgct gtttgtcctg acggtcttca
    14641 gccccgtctt ggagtatttt gtgctgggcg gaaaatatgc cgactccctg cctgtcggag
    14701 caggcggtat ggtcggcata cgtgtcggcg cagtgtttgc gtggctgttg gggaaatcgg
    14761 gcagcctgct gattatcctg gttgttctgc tgttgtcgtt gtccctgctg gtgcagattt
    14821 catggctgga atttttgaac ggtgcgggca gggcggttca aaaccgcctg agtgccttat
    14881 ccggcaaggt catggctttg agaaaacgcc ggccgaatac caaaacagac ggtgtcgata
    14941 cccaaaatac acggcgcatg gtgaaagaag ccaaaaacat cactgccaaa cccgttgcct
    15001 tgcccgaagg cagcagcagc aaccgcaaat ccgtcgcggt ttccgttgcg ccgccgccta
    15061 aaatccaacc ttcgctgttt gaagacaatg aagtacagca gaacggcgaa taccacaagc
    15121 ctacattgaa cctattgcgg attcccgaca gcgagccggt cagcatcaat cccgccgaat
    15181 tggagcgcac tgccgaacta atcgaatcca aactggcaga attcggcatc ggcgtacaag
    15241 tcgtatccgc gacatccggc cccgtcatca cgcgctacga aatcgaaccc gcgcaaggtg
    15301 tcaaaggcag ccaaattgtt gccctgtcga aagatttggc acgctctatg tcgctgcaat
    15361 ccgtgcgtat cgtcgaaacc attgcgggca aaaacacgat gggcatcgag ttgcccaacg
    15421 acaaacgcca agacgtgatg ttgagcgaaa tcttgtcctc gcccgtgttt gccgaagcca
    15481 aatccaagct gaccgtcgcg ctgggcaaag acatcgccgg tacgcccgtc gtcggcgact
    15541 tggcgaaaat gccgcacctt ttggtcgccg gcatgaccgg ttcgggcaaa tccgtcggcg
    15601 tgaacggcat gattatgtct atgcttttca aagctacgcc cgaagaagtc cgcttcatta
    15661 tgatagaccc gaaaatgctc gagttgagca tttacgacgg cattccgcac ctactctgtc
    15721 ccgtcgttac cgatatgcgc gaagcagggc aggcgttgaa ctggtgcgtc gccgaaatgg
    15781 aaaaacgcta ccgcctgctc tcccacgccg gtgtacgcaa tctggaaggc ttcaaccaaa
    15841 aagtcgaaca agccaaagcg gcaggcaagc cgctgctcaa tccgttcagc ctgaacctcg
    15901 acgaacctga gccactggaa aaactgccga tgatcgtggt cgttatcgac gaacttgccg
    15961 acctgatgat gaccgaacgc aaagctgtcg agcagcaaat cgcccgcctc gcccaaaaag
    16021 cgcgcgccgc cggcatacac atgattgtcg ccacccaacg cccaagcgtc gatgtcgtaa
    16081 ccggtctgat taaagccaac atcccgacgc gtatggcgtt taccgtgcaa agcaaaatcg
    16141 acagccgtac catcctcgac caaatgggcg cggacgaact gctcaaatat ggcgattcgc
    16201 tgttcctgca accgggcagt gccgaaccga cccgcctgca aggcacgttt gtttcagacg
    16261 gcgaagtgca tcaagtcgtc aactacgtca aatcgcaagc cccagccgac tatattgaag
    16321 gtctgctcag cggcgaagcc gcgctggaaa ctgccaatat cgttaatccg aatgcagaca
    16381 gcgacgaatt attcgatcag gcagtcgcct atgttttgga aagcaaaaaa acctccattt
    16441 cgtctttgca gcggcagttg cgtatcggct ataaccgcgc ggcaaacctg atggaggcac
    16501 tggaaaatgc gggtatcgtt tctccctccg acctcaacgg cagccgtaaa attttggcgc
    16561 acaaggacca tttatagccc gtatcgcaaa atgccgtctg aacggcggaa ttggcgtttc
    16621 agacggc
//
LOCUS       DAWTHD010000046        16625 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWTHD010000046 DAWTHD010000000
VERSION     DAWTHD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16625
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.046"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            40..582
                     /locus_tag="WM704_001131"
     CDS             40..582
                     /locus_tag="WM704_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFA9340561.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     EKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGVDGNTVLLADPSPGHVSMSRAQFLEAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            712..2373
                     /locus_tag="WM704_001132"
     CDS             712..2373
                     /locus_tag="WM704_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFA9340562.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(2507..3001)
                     /locus_tag="WM704_001133"
     CDS             complement(2507..3001)
                     /locus_tag="WM704_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFA9340563.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(2998..3828)
                     /locus_tag="WM704_001134"
     CDS             complement(2998..3828)
                     /locus_tag="WM704_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9340564.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            complement(3825..4721)
                     /locus_tag="WM704_001135"
     CDS             complement(3825..4721)
                     /locus_tag="WM704_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9340565.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            complement(4779..6134)
                     /locus_tag="WM704_001136"
                     /pseudo
     CDS             complement(4779..6134)
                     /locus_tag="WM704_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            complement(6307..8274)
                     /gene="nosZ"
                     /locus_tag="WM704_001137"
                     /pseudo
     CDS             complement(6307..8274)
                     /gene="nosZ"
                     /locus_tag="WM704_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            complement(8403..10709)
                     /locus_tag="WM704_001138"
                     /pseudo
     CDS             complement(8403..10709)
                     /locus_tag="WM704_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein NosR"
     gene            complement(10956..12203)
                     /locus_tag="WM704_001139"
     CDS             complement(10956..12203)
                     /locus_tag="WM704_001139"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFA9340566.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            complement(12356..12742)
                     /gene="gcvH"
                     /locus_tag="WM704_001140"
     CDS             complement(12356..12742)
                     /gene="gcvH"
                     /locus_tag="WM704_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFA9340567.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            complement(12794..13333)
                     /locus_tag="WM704_001141"
     CDS             complement(12794..13333)
                     /locus_tag="WM704_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340568.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            complement(13480..14580)
                     /gene="gcvT"
                     /locus_tag="WM704_001142"
     CDS             complement(13480..14580)
                     /gene="gcvT"
                     /locus_tag="WM704_001142"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFA9340569.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKVLQTA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     regulatory      complement(14791..14888)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            15092..15574
                     /locus_tag="WM704_001143"
     CDS             15092..15574
                     /locus_tag="WM704_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFA9340570.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     gene            15621..16226
                     /locus_tag="WM704_001144"
     CDS             15621..16226
                     /locus_tag="WM704_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFA9340571.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            16223..16582
                     /locus_tag="WM704_001145"
     CDS             16223..16582
                     /locus_tag="WM704_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFA9340572.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
BASE COUNT         3824 a         4431 c         4388 g         3982 t
ORIGIN      
        1 atgtttaacg ataatcctgt tgtttacgga aaaatcaaat tgcagagttg gaaagcgcgg
       61 cgggatttca atattgtaaa gcaggatttg gatttttcct gcggggcggc ttcggtggcg
      121 acgcttttga acaattttta cgggcaaacg ctgacggaag aagaagtgtt ggaaaaactg
      181 ggtaaggaac agatgcgcgc gtcgtttgag gatatgcggc gcattatgcc cgatttgggt
      241 tttgaggcga agggctatgc cctgtctttc gagcagctcg cgcagttgaa aatccccgtc
      301 atcgtgtatc tgaaataccg caaagacgac catttttcgg tattgcgcgg agtggatggc
      361 aatacggttt tgcttgccga cccgtcgccg ggtcatgttt cgatgagcag ggcgcagttt
      421 ttggaggctt ggcaaacccg tgagggaaat ttggcaggca aaattttggc ggtcgtgccg
      481 aaagggaggg atgccggtgg ggataaggcg tttttcacac gcagtccgaa acgtcagacg
      541 gagtttgcgg tcggacaggt aaaatggtgg cgtgcttatt gaaatttcga caaaggtcgt
      601 ctgaaaaccg aaaatatggt tttcagacga cctttgttgt atttggtaac tatatgttcc
      661 cgttgtataa tcacggattt gcaattcaat aataaataca caggaaccgc catgacagaa
      721 tccatcaccc gcgacagtat gcaatacgat gtcgtgattg tcggcgcagg cccgtcgggg
      781 ttgtccgccg ccatcaaact caagcagctt gccgaacaga acgggcgcga aatcagcgtt
      841 tgcgtggcgg agaagggttc ggaggcgggg gcgcattcgc tggcgggcgc ggttatcgat
      901 ccgattgcgc tgaatgagct gattcccgac tggaaagaaa aaggcgcgcc gctgacgcgc
      961 acggtaactc aggacaaggt gttgttcctg acggagaaaa aagcgttcaa tctgcctatt
     1021 accccgaatt tcgacaatca cggcaactac atcgtcagct taggcgaagt cgtgcgctgg
     1081 ttggcggagc aggcggaaaa tatgggcgtg gaaatctatc cgggctttgc cgccgccgaa
     1141 gtgctgtatc acgaagacgg ctcggtcaaa ggtattgcga ccggcaatat gggtgtaggc
     1201 aaagacggcg ggccgaccga cagtttccag cccggcatgg agctttgggc gcggcaaacc
     1261 ctgtttgccg aaggttgccg aggttcgctt tccaagcaaa tcatcgaacg tttccaactc
     1321 gaccaaaaca gccagccgca aacttacggc ttgggcatca aagaagtttg ggaagtgccg
     1381 tctgaacagc atcagcccgg tttggtggtg catagcgcag gctggccgct ggacagcaaa
     1441 acctacggcg gtgcgtttat ttaccatttc gacgacaaca aagtcgccgt cggcttcgtg
     1501 gtcggtttgg actatcaaaa cccgtatctg tcgccgtttg aagagttcca acgtttcaaa
     1561 acccatcccg aaatccgcaa aacctttgaa ggcggccgcc gcattgctta cggcgcgcgt
     1621 tcgctgattg aaggcggttt gcaaagcctg ccgaagctct cgttcaaggg gggcgtttta
     1681 atcggcgatg cggcggggtt cctcaatatg ccgcgcatca aaggcattca taccgcgatg
     1741 aaatccgcca tgctcgccgc cgaagccgtg ttccccttgt tggaaaactt tgaagaagtg
     1801 gaaagcttcg acagcggcaa agaagcggga aactatcaaa aactgtttga acaaagctgg
     1861 ctgtatcaag agctttatgc cgcgcgcaat gtccgtccgt cattcaaatg gggcgtttac
     1921 ctcggctcgc tttataccgg catcgaccag atgattttca gaggcaaagc cccgtggacc
     1981 ttgaaacatc acggcaaaga caacgagcag ctcaaaaaag ccgccgtgtg caagccgatt
     2041 gattatccga aacccgacgg tgtgttgacc tttgaccgct tgagcagcgt cttccttgcc
     2101 aatctcgcgc acgaagaaaa ccagcccgac catttggtgc tgaacaatcc gcaaacgatg
     2161 atagacgtga actataaaga atacgcctct cccgaaacgc gctattgtcc ggccggcgta
     2221 tatgaaatca tcgaagaaaa cggcagtccg cgcctgcaaa tcaacgcggc aaactgcgtg
     2281 cactgcaaaa cctgcgacat caaagacccg acgcaaaaca tcacttggat ttgccccgaa
     2341 ggcgcaagcg gaccgaatta cggcgggatg taaacctcat tccgccgtta cgcccggtca
     2401 ggaataagga atggggcagt tgttcgaacc tgccccgttt gaacctatga caaaaaatgc
     2461 cgtctgaacc ttggcttcaa cgttcagacg gcatttctgt ttggatttac ttgaaaatgt
     2521 aagcatcggg catatcgtca aaaccgacga ccttgccgcc tttatccttt gcaaattttt
     2581 cagcctgctc cttgttgccg aacggcagcg cgtcttccgc gcccataccg ccgataaagc
     2641 cgctgtcgat gacgtaaaag gcttttttcg catctatcca ctccgtgtcg gcattaggat
     2701 tcgtccaatc ggtaacattg cccatatcgg taacgtaaat cacgcggatg cctttgggct
     2761 cttcgggcag cttggtatag ccgaacatct gcttgacggt ggagaaccaa acgggctgat
     2821 cgggtttgcc gttcaaaaaa atctgggctt tgggaccgtt gtgttcggtc aggttcatac
     2881 tgcaatagtg tccgaccgaa cggtcgctaa tctgccgggg taaaggcggc ggtgcctctt
     2941 ccgcctgccg gcaggcactt aaggcgaaaa cggcaacaat tgccaacagg gttttcttca
     3001 tattcgtcgc tttctaaaaa ttccggctgc caaaacaagc ggaatgataa cccataaaac
     3061 ctgcgcggtc agcaaaacgg ggacggtcag gccgatttgt ccgctcaaac ccgccatacc
     3121 cgcatacata gccgtatttt cgtaaccggt caggttgagc aggcggtaaa tgtcggtggg
     3181 attgaacagc agcaccgttt caacgacggg cgcggtgatg acctgtttcg agtcggcaac
     3241 cagaataccc aaaagcgcca tatcgaagat gacgacgaaa aacaaccata cgccgatgga
     3301 aataccggcc gccgtccccc gctctttgac ctttgcacta atcaagtagc ccatagacag
     3361 aaatgccgca cctaaaatca cgctggcggc aatcaacagc gcaaagggtt tccaaacggc
     3421 gatgtcgaaa ccgccgttgg caagttgcag cgtaatgcct gccaatccgt aacctgccgt
     3481 ggtggcaagg gcaaggatga tgaggtgtcc gacaaacttg ccggcaagga tttggttgcg
     3541 ccaaatggga taactcaaca gcaacgccat cgtaccgcgt tcgatttcgc cgatcagtgc
     3601 gtcataagaa agcagcatcg caatcagcgg aatcaggaaa atagacaggc tcgacaggct
     3661 gacgacggta acggtcagcg gatcgacctt gaccgaaccg gtgggcgagc tgccgaggaa
     3721 acccaaagaa agggcgagtg cggcaagcag gaggacggcg gcaagcaccc agcggttacg
     3781 caggctgtcg cgggcttctt tgccggtaat aatccaaacg gggttcatac gtcctccctt
     3841 ttcaagaacc gtgcgtacat ttcgtcaagc gtcggcgtgt ggatgtcgaa ataggcaagg
     3901 tcggacaggc tgcccaattc gcccaacagt tccatacgtt cttcggcctg acattgcgcc
     3961 cggtaggaaa tgccgtctga aagcggctgc cagcggctgc tgagggtgcg cggcgcattg
     4021 aggcggatat tgacggtcag cggcagcccg ctttggacgt gcaattcgtc catactgccg
     4081 tcggcaacct taacgccgtt tttcatcacg acaatgcggt cggcgtgtcc gtccaactcg
     4141 gcaagggcgt gggtactgag caatacggtc gcgccgcgcc cgttgagttc gcgcacgact
     4201 tcgtaaaaca tttgtcgtga tgcagggtcg gggccggttg tcggttcgtc aaacagcagg
     4261 actttgggct cgcccagcag ggcttgtgcc aaggcaaggc gttggcgcat ccctttagaa
     4321 taagtgccga cgcggcggtg tgcggtctgg gaaatgccga cgcgctcaag cagcccccgg
     4381 ttctgcgtga gcggctgttt tttaagtttg gcataaaaat ccagcgtttc gatgccggtc
     4441 agcgaagggt gcagcgcaac ggtttcgggc aggtagccga tttggctgcg aagccgcgcc
     4501 cccgctttgc tgccggtacg ttcgcccaaa agcatcactt cgccttcggt cggggtaatc
     4561 agcccgagta tcagcttcat gatggtggac ttgcccgcgc cgttgtgtcc ggcaagcccg
     4621 acgctttctc ctgccttcaa aaccaaatcg acttggctga cggctttttg cgccccgaac
     4681 cgtttggtta ccttcctcaa ttcgacatga tgtgtggtca tggttgttcc ttttgtgttt
     4741 tcagacggca tttgaatccg ggtgccgtct gaagcagact agttcaaaga accgttttcc
     4801 gccctgcccc gttccgactg ccgcgtttcg gcttctttga gcaactcgtc cttcatcgcc
     4861 tgataacggg tttgaatttt gggggcataa ggcttcatca gcggtttgct gtccaccacg
     4921 ccgccgggca gaacggcggg aaactgcgcc tgcgcccatt tgacgatgct gattgcggga
     4981 ctgttcatca agaggcgcga tacgggcgcg cgccagatga tttggtcgat gatgccgtcg
     5041 ggacggtacg cgctgtctcc gaagccgtcg ccgttcaaat cgaacgggct gttgtcgctc
     5101 cagtagttgc cgtgtccgcc ctcgctccag tcgagaaagc gcgtactgac atatttgacc
     5161 tggcttccgt tgttgataaa ggaattgtcg tgcagggacg cgccttcgat ggcggcggta
     5221 aagtgcatgc cgatttggca gttttcaaaa tgattggcgg acagtttatc gtagttggca
     5281 ttgtaggcaa aaacgcactt gcccgctttg ttgataatat tgtcgtgaat atcggaatag
     5341 ttgacatagt tgagcatgat gccctaatcg cggctgccga cggcgatatt gtcgaacact
     5401 ttgagccgtt cggaaaacat cagcacatag cccatattgt tgcccacgca aatattgccg
     5461 ctgacttcgc tgtcgttggt gtacatatag tggacggcga aacgcaggtc gctgaagcgg
     5521 ttgtttttat aggtgttgtg cgtgctggta ttggaaaaaa tgccgtcccg ccctttggaa
     5581 atgtcgttgc cgacgacctg cgcgccgggc gcgttccaaa cggtaacgcc attgccgcgc
     5641 tcattcacgc gcaaggtcgc atcgccgacg attttattct cgcgcaccat cgcatcggca
     5701 gaaccatgaa ggtatacgcc gaacgaatta tcaaaaatat tgttgtgttc aaccagggcg
     5761 cgcggggcgg ctttttcgag ataaataccg gcatccattg ctggcaggct cataccggaa
     5821 cgggtaacgg tcaggttgcg gagcgttacg tccggcgcgt gtacggctat ggtacgcccg
     5881 ctcttgtccc cttcgatggt tgcggaacgg tcggcaggcc cttcaatcgt aatcggtttg
     5941 tcgatataaa gtttggtttg atatacgccg gaagccagtt tgatcgtatc gcccgcccgg
     6001 gcgcgggcaa aaatttcggc aaggttgtct tgcggggaaa cgtggacgac ggctgccgat
     6061 gccgtctgaa aaatgctgcc ggcggcaagt atcagcacgg ccttcagcca tatacggagc
     6121 gcggatgtgt gcatagtgtc cctctgtttc gttcggtatg gccgaacaaa ataaagcatc
     6181 attcaaatgt gcctgttttt atagcgaaac cgcctgaaac ggtacggcaa gcggtttggc
     6241 tataaatgcc gtctgaagac ctgttgcctt cagacgccat tctccatatt cagccgtcgg
     6301 gccgcattat ttgggatgaa caatcatctg accggacatt tccatatgca atgcgtggca
     6361 gaaccattgg cagtagtacc agtgcacacc cgggcggacg gctttgaagg ttacggaaga
     6421 agtggcttgc gggccgattt ccatagcgat gccgcagcct tccaaagtga agccgtgggt
     6481 caggtcctcg atggtttcga cgttggtaac gtaaacggtt acttcgtcgc cttggttcac
     6541 ttcaaactgc gggatactgt aagcaggcgc gacggccgtc atatacacgc gtactttgtt
     6601 gccttcgcgt acggctttgg cggctttttc cagctcgaca ccgtcttttt tcgcctgttc
     6661 caaagcatcc tgctagaacc acgggtcttt gcggtcccaa gttttgcccg ggttcagttt
     6721 ggacgcggca accaaacaca agtcgtgcgg ttcggcaaaa gtcgggttgt cgtgtaccag
     6781 acgcatttcg tcgccggaga tgtcgatcaa ttggtcgcac tcgggtttca aaggaccggc
     6841 attcaagaag cggtctttgg agaatttgtt caaggacacc agccattgac cgtcggcttc
     6901 tttggtttcg cccatggtcg tatggttgtg acccggttga tagtgaacgt cgagtttttg
     6961 tttgatcgga tcgattttct cgcctttgta ggctttgatc gcatcgtcaa tattccattt
     7021 caccatttgg ctgtcgataa acaatgtcgt ataagcattg ccgcgaccgt cgaatgcagt
     7081 gtgtaacggg ccgagaccca gttgcggttc ggctacgacc acatcgcgct ctttgatttt
     7141 gccggcgaac aaatcgtcca gtttgctgac atccaatacg gtaacggttg gaggcagttt
     7201 accgttgggc atgatgtatt taccgtcagg gcttgcgtta cagccgtgag gagagttcgg
     7261 cacagggata taacgcgtgt atttggattt ggcctcagcg cggccgtcca gcattttcac
     7321 accgttaact tctttgaagt cgcccgcttt gatgccttct tcgatggctt tcaggtcgaa
     7381 aacgacgcac cagtcctgct cgttggaaga cgcaccttgt acggtcagcg cgcgctcgga
     7441 gttgtagcag gtggcaaaag aatatttgcc ttgatagtcg gcatcgccgt tgtccaggtt
     7501 accgtcaacc aatacttgcc atgcgatctc catggtttcg ccgtcgatgg cggtgtacac
     7561 tgcattccag gttttcgcat catccagttt gcctacaccg ctgacagggg taatgtgttc
     7621 gccgttggca aaaacataac cggttttcgg ataacgttgc ggacgcagac cgtgaatacc
     7681 tgaatcatta gggatgtcga tgattttgtc ggccttcatc acatccaaac gcacgcggca
     7741 gacgcggttg tttgccttgt cgttggcata ggcatagcgg ccgtcataag tttggtcggt
     7801 aaacgacagg tgcgggtggt gcaagtcgcc gttggggtag cagcgcaggc cgccgtcttt
     7861 taagaattta cgggtttctt cggtaatatt gccgttgagg actttcaagc tctcattggt
     7921 gcgtccccaa ccggtcgcgc tgtccatatt gaacacgggg atacgcatca gttcgcgcat
     7981 agaaggcaga ccgatcaggc gcatctcgcc ggactggccg ccggaaagga aaccgtaata
     8041 ttgatcgagt tcgcccggac cgacttcgga agacagttgg ccgggtttgg attccccttg
     8101 cgccttggcg acgggagccg cgccggaagc gttagcggca gcctgttcgc cgtctttaga
     8161 gcaacccgcc aaacccaaca ggcccgcacc ggcaatacct gcaccggaag cggcggccgt
     8221 acctaagaac gaacgacggc tcaagccgtt ttgttctaat ttttcgtctg acatacaaca
     8281 ctccttggat aaaatacggc aaaccggttt tcggacggca agccgatggg aaaaaaattt
     8341 tattatcctt ccggggaaac cctgcctgcc tgacactttg gcttcaggct tcccccggat
     8401 gcttattttt cagattgcgc cgtttcgggt ttgacaaaac ggacgacctg ttcctgcggc
     8461 tgcttagaga cattttccaa ttcccctgat ttggcagcag cctgtttttg ttttttctta
     8521 tttgccgcca caacttgcgg acagcgcgta tcgtggtggt acatcacttg gcagtgcagg
     8581 cactggatac attcgttcgg atggatgtcg ccctcgggcg cgattgcctg aacggggcat
     8641 tcgtgcgtac agatttggca ggggttgccg cacattttgt agcggcgcag ccagtcgaac
     8701 acgcggaatc tgcccggcag cgcgatgccc gcacccaaag gacacagata acggcagaag
     8761 aagcgttcga taaacagccc cgcaatcaaa agtgccacgg caaaggcgac aaaccaccaa
     8821 tcgcacatga atttcaagat aatcgcagtt ttgaacggtt cgacttcggc aaatttttcc
     8881 accgtaccca aatcatacag cgaaattgcc aaaaaaccga agaaaatcag gtattttatg
     8941 acgttcaaac gggtatgcag catatgcggt acggtaatct gtttcacacc cagttttttg
     9001 gcaatgcggt tagtcaattc ctgtaatgag ccgaacggac acaaccaacc gcaaaacgtc
     9061 cccctgttcc acagcagcat ggttgcagcc gtgaacagcc acaggatgaa cacaatcgga
     9121 tccatcagga agaattccca atgaaattcg gtaaggatgg cggaaaacag cgtcagcgta
     9181 ttgacgaccg aaagctgcgc ctgtgcatac cagccgatat agaagagcgt aaaagttagg
     9241 aaagcaaagc ggaagcggtc gtaccatttt tcgtaacgca caatccaatc ttggaacagg
     9301 aataccaaaa gcaggatggt cagcgcaatg ccgacgacga caatctgccc ctgtttcgct
     9361 ttccaaatct gtttccataa ttggttggat acaccgtttt cagctgacgc atcttcggca
     9421 atgccgtctg aagcggtatc ggatgctgcg gcaggtactg cttcgacagg cgcgctgatt
     9481 tctacgggcg gcgctttagg atcatcgacg taataagctt ggggcagttc gtaatccaaa
     9541 tcggcggtaa cgaaggcttt atcgttcaca ctcaagacgc gctgaaccat caattacagc
     9601 cgccacggct ccgcaccgtc aaacgctacg ccttcaggga tggtaaacca agaaacttct
     9661 ttaaaacgcg gcgcatcggc ggcagacagc tcgacgacgc gttcgtgttg ggcatcggta
     9721 aaacggaagc tgttctcccc ctgaatcatc tcgatacggt cgaaaatacc gccgcgcaca
     9781 tagcccgaac ctttccaaga ataacggccc tctccggcaa ccaaaaccgc ctgctgcccg
     9841 ggtttcagcc gtttttgcag atgcgcccag ccgtcctcac ccagcaggct tttaccgatg
     9901 gaaggctggc tgaccaaggc aacatacaaa tcaataaagg tatcgtcagg atcgccctgt
     9961 tcggcgtgat cggccacgcc ggccttgccg cctttctcaa acagtttgtt gatttgatcg
    10021 agcgtgatat gcagatggcc gacggccttt tgtttcaaaa gttcgtccca agacaaaata
    10081 tcctgcttgt cgggatttgc catacggcgc ggacgggttt ctgacgcagg cgcggcttcc
    10141 cgaacatcgg aagcggatgc cgtctgaagg gccttgtccg aacccagacg gtattggttg
    10201 gcaatgacct tgtacgaacg ctggatgctg tcgttaacca ccatcagtgt aacagtcgca
    10261 ccgctgatga tgtcgcccgg cgccacgccc ggggtcggcg gatttttaat aaaattcaga
    10321 ccgatatatt tgtcgatgaa cttatcgaca cgcgattgcg ggataccgat cagcataatc
    10381 ggttcgtgat gatcgaccag tttcgccccg gctatcgtgc cgtcgtttgc caaagccatc
    10441 agcgtatcga tcggtttgct cgaataaccg cgcgtattga ccgcatcggt cgtgatataa
    10501 accaaaccga gctgctcatc gcctttgtaa acgcgggcaa ccataggctt gccttccggc
    10561 ttgccgtaac ggtccgcacc cggaaaaatt tccgaaggct gtattttcgc cagaaaatca
    10621 ggcagacgct ccgcataagc gggcagtgcc gccgtcaaaa agacaaaggc ggtaaaaaac
    10681 gccgttatcc gcgcccgaaa cgcagacata cgccggattg aaaaacagga catagctatc
    10741 cccgatatac cgatactctt taatattgat attatatgaa tcatctatcg gcgatttttg
    10801 atgcaaatca aatttacaaa tcttaaaacc aatccgcata taggataata ctttgtaatt
    10861 acatcgatat aatgtgggat ttcacgatgt tgtcaggcgg cacaaaaata tgccgtctga
    10921 aaccccttca gacggcatcc ttttccggcg caccgttatt tgtccaaatg ataaatctgc
    10981 gcgacggcat gaaccaaatc tttatcttct tcccccgcct tattcaaggt ttgagttggc
    11041 gaatgcagca gcttgttggt cagttggacg gacagccgtt ccaaaacctc ttccgccgtt
    11101 gcgcctttgg caagctgttt catcgcattt tccaacacct gcttgcgcgc tttctcgccc
    11161 tcgtcccgca aggccttaat cagcggaacg ctctgcctgc cctgctgctg cctgacaaat
    11221 tcggcaacct tttcggacac cagcgtttcg gcggcggcgg cggccttctg ccttgcctcc
    11281 ttgccgcttt ggacgatgtt gaccatatcg tccaccgtat aaagatacgc atcgttcaaa
    11341 tcgccgactt ccgcttcaat atcgcgcggc acggccaagt caagcatgaa caacggcata
    11401 ctctgacgct gtttcaatgc gcgttcgacc atgcctttgc cgactatcgg aagctggctc
    11461 gccgttgaag aaaccaccac gtcgtaatcg tgcagaatgg caggcagatc ggacagcagg
    11521 cacggttcgg cgttaacacc gagcttgtcg cacaactcct gtgcacgcgc cagcgtccgg
    11581 ttggcaaccg tcatcagccg gggatttttg gcggcaaaat aagtggcaac cagctcaatc
    11641 atttcgcctg cgccgataaa caatacgttc aaatcgccga tgtcgggaaa aatctgttcc
    11701 gccaacttga cggacgcgga agccatcgaa accgaatttt cgccgacagc ggtatcggta
    11761 cggacttctt tagcaacgga aaaggttttt tggaacaggg cattgagttt tgcccccata
    11821 ctttcctgtt cttgagccgc acgcaccgca tctttaatct gccccaaaat ctgcggctcg
    11881 cccaaaacca tcgaatccaa gccgcaggca acgcggaagg cgtggcgcac ggtttcctgc
    11941 atatccagcg tgtacagata cggacggatt tcttcaatcg gcaaactgtg gtaatcggca
    12001 agccatcgga tgatttcttc cgaatcgccg acgcagtaaa gctcggtgcg gttgcaggta
    12061 gaaaggatta ccgcctccgt tgccgcattg cttcgggcaa gattgcggac ggcttctggc
    12121 agggcggcgg cggcaaacgc cagcttttcc cgtatgctta aaggtgcggt ttgatgattg
    12181 agtccgacag cggtaagttg catgaaaaaa tcggtgaaga tggatataga ccgttattat
    12241 agcctaactg catccttaaa acacggacaa aaaaactgca cctccgaccg cagaaatgcc
    12301 gtctgaaggt gcagtttccg tttgtccgac ggttgccgtc ggacaaacgg gcggtttaat
    12361 ccacttcgcc cgcgtattgt tcggcagtca gcagaccgtc gtaatcggca ggattggcag
    12421 gtttgatttt gaagaaccag cctgcgccgt aagggtcgct gttggcggtt tccggcgcgc
    12481 ctggcaagtc atcgttgacg gcaacgactt cgcccgcaat cggcgcatac acgtcggatg
    12541 cggcttttac agactcaacc acaccggact gttcttcggc ggcgaggttc gcaccgactt
    12601 cgggcagctc gacaaacacg atgtcgccca acagctcttg cgcgtgatgg gtaataccga
    12661 cggtaatgat accgtcttct tcaaggcgca gccattcatg gctggcaacg tatttcagtt
    12721 ctgttgggat gttgttgctc atggtttgat tctccattgg tagggggtta ttttcagacg
    12781 gcatctgcac aacttagcgt acgcagatac ctcgtgcgat taattcttcc aagcgtccgt
    12841 aatggacggc aatcatattg tttttatctt gttccaattc ttgcttttgg gcggaaggca
    12901 gtttagagga tgcagaataa tccaaatatg cttcgtttat cttgcgctta ttacttgcca
    12961 ttacgccgct tgcacggaat acaacgattt cttgtttggg cctcatattg tctgcaacgg
    13021 caaccttcag gcctttgctg cggtaatctg cacaaatgcc tgtaacagcc gatactttat
    13081 ccacttcaac tgtgcccgca taacggctga acgcaattag ggtaaatgat ttcgcttgcg
    13141 acaatgcttc caggtcggaa gcacggcttc tacccttatt atcttgcacg gcaatactca
    13201 aatcatcatt catgcggaca atatcaagag catattcccc gtctatcacc acctcgccgg
    13261 caatctttcc attgccgcca acgattttca taaatattgc agtatgggat tttccttctg
    13321 tatcgaaaaa catcaatccg cgctcgccgg cgacgaagcg caagccgata acatcaataa
    13381 caacaataaa ctgccaaaat ttgctttcat aggaaataac aattccttaa aacctgcttt
    13441 cagacggctt aattcaaaca aatgccgtct gaaccgatat taatcaaact gtttttgacc
    13501 gctgcggacg aacggcagct tcagtacgcg cacgtccgct tctttgccgc gaatcaggac
    13561 tttggcggta tcgccgtcaa aatctttcgg tacgcgggcg atggcgatag attgtttcag
    13621 gctgggcgag aatacgccgc tggtggtttc gccttttcct ttgtcggtca acacttccat
    13681 atgcgcgcgc aggatgccgc ctttttcgag cagcaaaccg acttgtttga cggcaacgcc
    13741 tttttctttc aatgccaaca aggcggcttt accgacaaaa tcgcggcttt cgtctttcaa
    13801 atcaaccgtc cggcccatgc ctgcttcgag cgggctggtg tcgtcgtcca tatcgttgcc
    13861 gtagaggttc ataccggctt ccatgcgcag ggtgtcgcgc gcgccgaggc cgcaaggctg
    13921 tacgcctgct gtttgcagga ctttgaagaa tgcgacggct tcggtatccg gcagaatcac
    13981 ttcgacgccg tcttcgccgg tgtagcctgt acgggcgaca aaccagtcgt tgcccaaatc
    14041 cgcgccttgg aacggtttga ggccgtgaat cacatccgcc cattcgggtt tgacggtcag
    14101 gagtttttca atggctttag gaccttgtac ggcaagcata ccgaggtcgt agcgcggatt
    14161 gaaggcaacg ccgaactctt gtccgacttt gtggaattgt gccgtgtctt tttcacgggt
    14221 cgcaccgttg gacacgatgc ggtattgggt ttcggcctcg ttggtgcggt aaacgatcaa
    14281 gtcgtcaatc acgccgccgt tgtcgttgag caaagcggaa taaagggctt tgccgacaaa
    14341 agcgagtttg gcaacgtcgt tggcaatcaa tttgcggaaa aaggctttgg cgttcgctcc
    14401 gacgacgtcg gtaacgagca tatgggatac gtcaaacata ccggcgtcgg tgcgcacggc
    14461 ttcgtgttcg gcgatttgcg aaccataatg gatgggcagt tcccagccgg caaaatctac
    14521 cggcttcgcg cctgcatctt gatgggcttg atgaaacggg gtggttttca gagcagtcat
    14581 tctcaagttt ctccggattt tttgttcaga tgccgcccgc gcacctgtgc gcggatgacc
    14641 ctatctgtcc ttgaacctga gattttcggc cgtatccgca tgatggcaaa ccagtctgct
    14701 cactgctttg cgcggttgtc tgaatggtgg ttcagacggc cttctcccct tcggtggacg
    14761 ttattctaac gccgctctcc agattgtttg tttcaatgtg cagtccctga tacctgagcg
    14821 atttaagggt gtctgcgcct tcggtggtta cattgtagtt gggtgtaaca ctctcctgca
    14881 catttcccga ttatgtcttg aatcggcggt ttgggcaagc cgtcttaaaa gagatgcaaa
    14941 ccgaccgcct ctgttttcac atgattttcc gaaaacaaaa tttgtcccgg atgacggtat
    15001 aactttttat atgttaatat ggtctccatc tatgcggatt gccgcaaaca tacggacaga
    15061 caatccgaat tccttaaaac ggaacgcaaa tatgccccaa ctcactttag acaaaaccga
    15121 tattaaaatc ttacaggtcc tccaggaaaa cggcaggctg accaatgtcg aactgtccga
    15181 acgtgtcgca ctttcgcctt ctccgtgcct gcgccgtctg aagcagttgg aagatgccgg
    15241 catcgtccgc caatatgccg cgctgctgtc tccggaatcc gtcaacttag gtcttcaggc
    15301 atttatccgc gtttccatcc gcaaagcaaa agacgcgcgg gaagattttg ccgcatcggt
    15361 tcgaaaatgg ccggaagtct tgagctgctt cgccctaacc ggcgagaccg actacctgct
    15421 tcaggcattt tttaccgata tgaacgcgtt ttcccatttt gttttggata cgcttttatc
    15481 ccaccacggc gtacaggatg cgcaatcgag ctttgtttta aaagaaatca aacacaccac
    15541 ctccctgccg ctcaaccacc tgctgaagga atagtgccct tcccgatttt cccgcacgtg
    15601 cactctgcaa acacatttac atgacaataa tgaatatcga tacttccgaa aataaagatg
    15661 ctgttgccga acacaccgga caatggttgg aaaaagccgt catcggtctg aacctgtgtc
    15721 cctttgccaa agccccccac gttaaaaacc ttgtccgcat cgcgatcagc gaagccaaac
    15781 accttgacgg ttttttggaa gacttggacg aagaactgca gcgactgggc aatacacccg
    15841 ccaccgaact ggaaaccacc ctgctggttc acccgaccct attccccgat ttcgacgtat
    15901 tcaacgatat gctcgacatt gccgatgccg ccgttgtcga aaacggcttg gaaggcatcg
    15961 tccaaatcgc cccgtttcat ccctatttcc aatttgaagg cacggattca gacggcatcg
    16021 gcaactacac caaccgttct ccctatccga cgctgcacct catccgcgaa gacagcattg
    16081 ccaaagccgc acaagccttt cccgacgctt cggcaatatt cgaacgcaat atcgccctgc
    16141 tggaaaaaat gggacatgaa ggctgggcaa aactcggtat cacatcctgc ccttatccgc
    16201 acaataagaa aaatatttca aaatgatccg ttatctttta attgcctgcg gcggcatctc
    16261 cctgctgttg gggataatcg gcattttttt gccgctgttg ccgaccacgc cgttcgtact
    16321 actctccgcc gcctgctggg caaaggcatc cccgcgcttt caccgctggc tgcaccggca
    16381 ccgctatttc ggcccgatgg ttcataactg ggaacaaaac ggcgcagtgc cgcgcaaagc
    16441 caagattttc gccatcagca tgatgaccgc atcctgcctg atgatgtttt ggcagtttcc
    16501 ccaacgctgg tgggtcgggg cggtttcatc ggttttttgt tcccttgtcg ccatatggat
    16561 gtggcgcaga cccgaatctt gaatgccgtc tgaaagggat aagggcaaac aaacgggtgg
    16621 ggcaa
//
LOCUS       DAWTHD010000047        16563 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWTHD010000047 DAWTHD010000000
VERSION     DAWTHD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16563
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.047"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..916)
                     /locus_tag="WM704_001146"
     CDS             complement(<1..916)
                     /locus_tag="WM704_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9340573.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVIL"
     gene            complement(966..1196)
                     /locus_tag="WM704_001147"
                     /pseudo
     CDS             complement(966..1196)
                     /locus_tag="WM704_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(1245..4250)
                     /locus_tag="WM704_001148"
     CDS             complement(1245..4250)
                     /locus_tag="WM704_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFA9340574.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HAGKPEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQTVYTKIKEPNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALNGVKTDADQRVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQTINRLLVREYLKESGRI"
     gene            complement(4370..5680)
                     /locus_tag="WM704_001149"
     CDS             complement(4370..5680)
                     /locus_tag="WM704_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFA9340575.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            5951..6928
                     /locus_tag="WM704_001150"
     CDS             5951..6928
                     /locus_tag="WM704_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340576.1"
                     /translation="MELITTGEGADGDLLVNGSYGIRSFADLKLEYKPELSVRSNHDI
                     IFNNDFQSVDVQPYSGNIHLDAKGKIQFNLNVADKVTDPTTHQEKLVNDNALVRYVNN
                     ITPSDENDKGSISFHSKTGNLFNMNVNVHKGTHHFGIHNLRNTNVNLTAEDGDNVVAI
                     KGKLAEPMPAGKVESTSFLRGIETAFKSNTVLTGNNNKISITTEGDGLGLGVNGLQSN
                     SPYSGHTTGTFINNQPSNTTQPSLDGTSTIELKAVKGNNEVDLNVKNHASVKGIITSH
                     SAKATLEAEEDNIVRVKNPDTKTALINAWKEKYPNNSPEPYRVGGSIHD"
     gene            6858..7511
                     /locus_tag="WM704_001151"
     CDS             6858..7511
                     /locus_tag="WM704_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340577.1"
                     /translation="MPGKKNTPIIRQNHTGWGVQSTIDSDVVLSAANNIVEVGGSPMN
                     QEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTGARPGTRDDGNKIILEA
                     DGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAYGDKSLVKLIGENNIVK
                     SEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIWGLYSNNSSISLKGKK"
     gene            7629..9233
                     /locus_tag="WM704_001152"
     CDS             7629..9233
                     /locus_tag="WM704_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9340578.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGINVNLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            9264..9735
                     /locus_tag="WM704_001153"
                     /pseudo
     CDS             9264..9735
                     /locus_tag="WM704_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9761..10677)
                     /locus_tag="WM704_001154"
                     /pseudo
     CDS             complement(9761..10677)
                     /locus_tag="WM704_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10932..12396
                     /locus_tag="WM704_001155"
                     /pseudo
     CDS             10932..12396
                     /locus_tag="WM704_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            12647..13015
                     /locus_tag="WM704_001156"
     CDS             12647..13015
                     /locus_tag="WM704_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340579.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            13088..14497
                     /locus_tag="WM704_001157"
     CDS             13088..14497
                     /locus_tag="WM704_001157"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFA9340580.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            14544..15338
                     /locus_tag="WM704_001158"
     CDS             14544..15338
                     /locus_tag="WM704_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340581.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            15526..16302
                     /locus_tag="WM704_001159"
     CDS             15526..16302
                     /locus_tag="WM704_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFA9340582.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
BASE COUNT         4169 a         3861 c         4281 g         4252 t
ORIGIN      
        1 ttaatattac ttgtgtacca tctgctcccc gcgcatattg aaaattaact aacttaacaa
       61 ttcttgtatg atcaccaaaa ataatatgag catcttgtgg gtttaatata gatttttcat
      121 cattggtaaa tccacaaata aaatcttgac tttggtcaac aacaggatat tttccaaaat
      181 ccttaatatc tttatttaat atttttgtag tatttgggat ttttgctaag caagattgta
      241 attctatact tccccacccc ttcggaattt cccgtttcaa ggtttcgtca aacaccatgt
      301 cgccgcctga ggatttgtag ggcttgccgt tggcgtcggg gaaatcgaac tgcacaaacc
      361 agtagtcgta cagggttttc gccatctctt ccaggcgcgc gttgatttgt ttgttcaggg
      421 cgattttttt gtccaaagcg gataggacgg cggcgatgga ttgttggatt tgaggttctg
      481 gaataggcaa ctccagtgtt tttaatgcat tttcattgac acgttgcctg ccggaagttc
      541 cttccataca ttcaatcgct cgttttctaa aatcgggact gattgcaaaa taatagagaa
      601 attcgggatt ggtttcattt tttgctctta acacgataaa ttcggtagaa ccgaaagcca
      661 cttctccatc atctaaaata tctacaaaag cggttttccc attttccaaa cacggggtaa
      721 tcttagctaa taaggtatca ccattgcgaa acttagctcc accgttaaat gccttgattt
      781 cgtaacccgt aatttgtcgt tgaaattctt tcaacattgc catcggaaca ctttttgcca
      841 atgctccctt ggctagttgc tctctcggat taaaatcagc aatattttga atttgttgtt
      901 tcttaaaatg attcatgatg ttccgttaca aaactcttca aaggccgtct gaaaaaaaca
      961 tttcatcggt agttcttgcc gtctgcggca gttgtaaact gaaagtttac aactgaatcg
     1021 tccagttctt gttctgcaaa tatggctttg atgtgtttac cgatgttttg tttgctggtt
     1081 tggtagagtt cggcaatttc tttttgggca agccatagtt cgccgtcaag ctcgcgcagg
     1141 gtaaattcgg atagtccgtc ttccgtggtg tagatgatga tgtcgttgtt gtgcatagct
     1201 ttcctgtatc tataaatggt taaggctacc tgaaaatcta cttctcaaat ccgcccgctt
     1261 tcttttaaat attcccgtac caacaggcgg ttgatggtct ggatgtctgc gggttgaaca
     1321 ggaaattgct gttccgttct aaagcgtttt aatacgatgc cctgcatttg tttttcaaag
     1381 taattctggt tgtccaaaat ctgctccata tccagcactc tttggtcggc gtcggttttg
     1441 acgccgttta gcgcgttgaa gactttgagt ttgtctccgt agagggcggc gttttccatc
     1501 aaccgcttgt ggatacgtgc gtatttggcg tcgccgccgt atttgtgccg caagaggtcg
     1561 ttttgtcggt tcggctcttt gattttggta tagacggttt ggagtgtggc aatgtttgcc
     1621 tgcatttctt cctgcccgac ttcggcgagg ttctttttct tgaagatgcg ttccagctct
     1681 tcgcgcaggc tgatgtattc aaggtcgtct tggtcgaagt tgcccgccag cccttcgcgc
     1741 actttgcgca tgatgtcttt taaatcgtcg gcaagtttga gttccgcctc gccgattttg
     1801 acgaattgaa agtaaacgtc ttccagcgct tcattgagca aatgggcggt gtcgccttgc
     1861 tgtaattttt cggcaaggtt taaggtatcc aagcgggcgg cggtttcgcg gtacaaaaga
     1921 ttgagcttgt caaaatccag atgcgcaaga aattcgtggc tgccttgcag gcgcaggatg
     1981 ttgtacaact ctttggcggt ttgcagggct ttttttaagg ccagaagctg ttttttgtct
     2041 tctatttggc taatttggct gcaaaattct tcggcgtttt cggtatcgaa atcaaacagg
     2101 gcgtttttaa tgtctgcaat ttcctgttcg atttcttcgg cggttttgaa tagctggctg
     2161 taactgccga tttcgtcgcc caattcgttg gaaagttcgt cccaataggc gcggttggtt
     2221 ttgtcaaatt cgcgttcgat atcggcaaaa tcgacgacat agccgtagcg gtaagatttg
     2281 taggtgcggt tgacgcgggt cagggtttgt aaaaggttat gggcttttat cagtctgccc
     2341 aaatagagct ttttcaggcg cggcgcgtca aagccggtca aaagcatgtt gtacacaaac
     2401 aagatgtcgg ttttgcccgc tttgaaatct ttaacccatt ggtcgcgttc ttccttagtg
     2461 ccgacgtcgt gcaatatcaa cgcggcggtg aaattgtggt ctgaggccgt ctgaaaatgc
     2521 tcaaaaagct ggcgtgcctg ttctgcgctg tcgcaaacga ccatggcacc gaggctctcg
     2581 tcttggttgg tttttctgaa tttggcgaag tcgtccaaga tgtagtcaag catcgggtgg
     2641 acgaagtgcg gatgggcgta gatttctttg cggtcaaagc tgcctttttc gatttcaagt
     2701 tgcgccagtg cttcttgtaa ttgcgccttg tatcggctgc cgatttcttc gcgtatcagg
     2761 cgcagggtgt agccgtcggc aatggaggcg ttgtaatagt atttgtggat gtagtcgccg
     2821 aagagttcgc gggtgttgac gttgcctgcg gttacgccga taagcggcgt gccggtcagc
     2881 ccgattttga cggcgtttac gtcggactga ttaaggttgg caagaaatga gcctttgggg
     2941 ttgtagctgc ggtgtacttc gtcgagaaaa tagacgcgct gaatggcgag gtcgtagtcg
     3001 ttacgggcga cgacatcggg gtcgtcttgg aatttttgga tgttgacgac ggtgatttcc
     3061 ggtttgcctg cgtggttgtg cagggtttgg gcggatttga tgtcggcagc gaaggcttcg
     3121 cggctgtcga tggtatggac gattaagtcg cgggcggtaa attcccgctg cgcctgtttc
     3181 aataaatcga gcctgtccac gatgaaatag aatttcggca cgataccctg tttggcgtaa
     3241 tagtgggtca ggtaacgggt gttgtaatag gcaggcgcgg ttttccccga tccttgggta
     3301 tgccaaatca cgcctttttt accgccgttc gataaatgtt tttcaatggc aagggtggca
     3361 aacagttgcg gataacgcat gatgtgtttt tgcaccgaac cttgacttgc tttgacgtaa
     3421 gtcagcccgt gttgcaggag aaaggcaaga cgttcgcggc aaagcagcga agtcaggatg
     3481 cggttggtcg gcgtatcggg cgatttattg ctgataaatt cggggctgtg tttgataacg
     3541 ggcagattat tgtcttgtag aacgttgttt tcaaggtcgt ctgaaagcgt gtccaataat
     3601 tcggtaaggt ttaatttatg ctcttcgcgg aagtagttga atacgggctt gccgcaagca
     3661 gacgaggcgt aaaacgcgcc ttgtgccggc tcggttgcgc cgtcgtcgta ttccatgttg
     3721 ttggaaaaaa tcatgaattg ggtgatgttg ataaaacggc ggaatttggg atttttcgca
     3781 cgtttgccca tgcgctcccg ctcttcgccg atgccgcctt tattgttggg ctttttgact
     3841 tcgataaaga ccaaaggcat gccgtttacc agcagggcaa tgtcggggcg gaatgcttcg
     3901 tcgccgttga tacaaggcaa ctcagttaca acgtggaaac tgttgttgtc aaaattttgg
     3961 aaatcgatca gcttcttacc gccggattga ttggtgaggc gttcgtaaaa tttttgcccc
     4021 aaatcttcgt tatccagctc taagcgtatg tcggcaagca ggcggcgtgc atcatccggc
     4081 ggcaaatcgg gattgatgcg gcagaggctg tctacaaaga tttcgggaaa gatattggtt
     4141 tgtctgtccc aatttgcatt ttttagcgaa agataatcat agcccaagcg catcaggtgc
     4201 aaaacggcgg ggattttgac gcgggaattt tcgttgtggg tttgggacat aaacgtaacg
     4261 taatgggtca tcaaaattta ttgaaacgga ttataacacc tgaaaaaagc cgatacgcct
     4321 tttcggtgta ccggctttgc catactgttc tgcttcagac gacatcgctt cattttgcct
     4381 ttaatgcctc ttcgtccagc gatttcaacc atgccagctt ttcgccgatt ttgatttcca
     4441 gcccgcgcgg gacgggttgg taaaagtccg gttcgtccaa gccgtcgggc atataacttt
     4501 cgccggcggc gtaggcgttc ggttcgtcgt gggcgtagcg gtattcgcgt ccgtagccca
     4561 attccttcat cagcttggtc ggggcgttgc gcaggtggac gggcacttcg tcgctggcgt
     4621 tttctttgac gaagcggcgc atttggttgt atgccttgta gcccgcgttg gatttggcgg
     4681 cggcggcaag gtacagcacg gcttgcgcca gcgcgagttc gccttcgggc gagcctaagc
     4741 gttcaaaggt ggcggcggca tcgttggcga tttggaaggc gcgcgggtcg gcaagcccga
     4801 tgtcttccca agcgatacgc acgatgcggc gggcgagata gcgcgggtcg gtgccgccgt
     4861 cgagcatacg gcaaaaccaa tacagcgcgg cgttcggatg cgaaccgcgc acggatttgt
     4921 gcagggcgga gatttggttg tagaaactct cgccgccttt gtcgaaacgg cggatttgcg
     4981 cgcccaagct gtcggcgaga aattcggtgg tcaggatttt cagacggcgc gtgtcggcgg
     5041 cacgtaaaag ttgttccaac aaattcaata atctgcgcgc atcaccgtcg gcggtattga
     5101 cgagcagctc ttgcacatcg gcttcaatcg tgaaatcccg gtattcgggc aaagccaaca
     5161 ctttggcaat cagtttcttg aggtcgtctg aagacaagga ttgcaaaaca tatacctgcg
     5221 cgcggctcag cagcgcggga ttgacttcaa acgacggatt ttccgtcgtt gcgccgataa
     5281 aggtcagcaa accgctttcg acatgcggca aaaacgcgtc ctgctgcgcc ttgttgaagc
     5341 ggtggacttc atcgacaaac aaaatcgtcg cgcgtccctg ctgcaaagcg atttcagcct
     5401 tatcgattgc cccgcggatg tccttcacgc cggaaaacac ggcggaaaca ggcaaaaact
     5461 gggcattgaa actctgcgcc aaaatccgcg ccaacgttgt cttgcccacg cccggcggcc
     5521 cccacagcaa catagaatgc ggcttcccgc cttccaccgc cacgcgcagc ggcttgcctt
     5581 cgccgattaa atgctgctgc ccgataacat catcaagcgt atgcgggcgc agacgttcgg
     5641 caagcggtgc gtcgggttct cggacaaaca aatcggtcat aacggctccg tcaacaggtt
     5701 ttcagacaat atgattatac ggcagggaaa ggcggcgtgc cgcatacgga taccgcccca
     5761 ccgtttgtac taagccgatc ttaggcgtac aattgggaaa tttcaggaaa tttatacgct
     5821 atgccatcca tgcagctttg caaccggacc cgcaaagacc aggtcccgtt ttaaccatgc
     5881 ggggaagtta agattacagt aaacaaaact attttgcctt attggaagaa aggggaacag
     5941 ttatgagaaa ttggaactta ttaccaccgg ggaaggggca gatggagact tactggtcaa
     6001 cggttcttac ggcatccgtt cttttgcaga cctaaaattg gaatacaaac ccgaattatc
     6061 ggtgagaagc aatcatgaca ttattttcaa caatgatttt caatctgttg acgtacaacc
     6121 ctattccgga aatatccatt tggatgccaa aggaaaaatt caatttaatt tgaatgttgc
     6181 cgataaggta acagatccaa ccacacatca agaaaagtta gtgaacgata acgctttagt
     6241 gcggtatgtc aacaatatca ccccctccga tgaaaatgac aaaggcagca tatccttcca
     6301 ttcaaaaaca ggcaatttgt tcaatatgaa cgttaacgtt cataaaggca cacaccattt
     6361 cggtattcac aacctccgca ataccaatgt taatctgact gccgaagacg gcgataatgt
     6421 ggttgcgatt aaagggaagc ttgccgagcc tatgccggca ggaaaagttg agagtacaag
     6481 ttttttaagg ggtattgaaa cggcttttaa atccaatacc gtattaacgg ggaataacaa
     6541 taaaatatcc atcaccactg agggcgacgg cttgggattg ggcgtaaatg gcctccagtc
     6601 caactccccc tattccggac acacgacggg tactttcatc aataatcaac caagcaatac
     6661 tacccaacct tcccttgacg gcaccagcac catcgaactc aaagccgtca aggggaataa
     6721 tgaagttgat ttaaatgtaa aaaaccacgc ctccgtaaaa ggcattatca cgtcgcattc
     6781 tgcaaaagca acgcttgaag ccgaggagga taatattgtc cgggttaaaa atccggacac
     6841 caagacggct ttaatcaatg cctggaaaga aaaatacccc aataattcgc cagaaccata
     6901 cagggtgggg ggttcaatcc acgattgaca gcgatgtagt tttgagtgcg gcaaacaata
     6961 tcgttgaagt aggaggcagc ccgatgaacc aagaagggat taccgctcac ggaaatgcca
     7021 cgattaccct caaggcgaaa gaaaacaata aaattaccgt ggaaaacgcc gcatacagca
     7081 gcgacggcat ttcgactctg attaacagaa cgggggcaag acccggaaca agggatgatg
     7141 gaaataaaat catactggaa gccgacggcg ataatattgt taccatgaaa tccggcgatg
     7201 cggatgcgga ttatgtaaac aattccaaag tattaacgga gacaccatat tataaaagca
     7261 aacgaggttc caacggcatt tttgcctatg gcgacaaatc gctggtcaaa ctgattggcg
     7321 agaataatat cgttaagagt gaaatcagtg aaaaatctaa ggcattaaat gggggatttc
     7381 gccatatcgg catttattca tggcaaaacg cgaaagtcga attgtctgcg aagagcgaca
     7441 atatcgtaca aggcggaatt tggggcttat actccaacaa ctcctcaatt tccctcaagg
     7501 ggaaaaaata atgtgatttc aaacccgaaa tataatgttt tcgcctacaa aaaggcaaag
     7561 gtggatttgg tcaaattacc attgactcca acgttgccaa tcttgcaagg caagatggtt
     7621 caattcattt gaattataaa gacgataccc gtatcacagg ggcaaccgta tctgataagg
     7681 gtttggtagc catcaaacct ttgaataaca cgaatattgt tgccgacact attcactata
     7741 aaggcgatgt cttggcggta aataagggta aagtggaatt agatttcacg ccgaacatcc
     7801 gtttagtggg acgtttggat aattttagcg gcttaaccga ttccaaacat aaaaatttat
     7861 tcgaaaacta tgttgcaaat ttagacagca aaagtgcggg cgaaattaac tttaatttag
     7921 ccaaagacgc attatggacg atgacaggtc aaagctggct ggataaattg gaaggacaag
     7981 gcactatcga ttttaataat gatgctaaaa caagtggacg cgccttacat atcggtgaat
     8041 tggcgggtgc caataaattc ttgatgcatc tgaataaaga cggcattcac agcgatatgc
     8101 tctatgtgaa aaaaggcact tcgacaccgc aagaagtcgt cgtcaaaaat ctgtccgaag
     8161 tgctcgacag tatgaattac ggcgaacgtt tgcgtttcgc tacagtaaca aactcaaaaa
     8221 atgaatttgt gaacggtaaa aaatatattg acgatacgca ccttatggag gatgccctga
     8281 ctgtcgaata ctccgcacat aacggcgata aaaacaacaa ggatgactat aataaatcct
     8341 ttaacggctc tgaaatgacg gcggaaaaag ctggagacga ttatgtcaat aaaacctata
     8401 ccgacaacag gcaaaatgtc tatttggtca aacaggctac cggcaatccg agccgaaatg
     8461 tcaaaaatat caatgatatg ttcgattcaa ccgcacatta tgcgttcact ttggatactt
     8521 atgccaaacg cgaaggggag cgggcttttt caacgttgga taaaaaagaa ggcgattgga
     8581 taaggctgac gcatacccgt gtgattcaat ccaatgcgtt taggtttcat aacaacgatt
     8641 ttgaaatcgg atatgaccga ttcagcctca acgagcagga gaaaaaacgc aaatggggca
     8701 taagtctcga ctacggccac ggcaggacat cattatggaa tacgtttggc aaggacaaaa
     8761 tcaggaaata tgaattggct ctgtacaata ctacccaata catagataaa gaaggagacg
     8821 aaacagggta tatcgacaat gtattaaaaa taggaaaact gcgtaaccgt gtgattgcac
     8881 gaaatcatat ggggcaatta tggggcaagg gaaaatatag caacacccta ttctctatca
     8941 gcaccgaata cggccgccgt aaatttttgg atgacgataa attgtggcgg attacaccgc
     9001 aagtacagtt gcaatattcc tatttgagag gtaccggcta tcggatcgat aacggcataa
     9061 acgtcaattt aagccacgca aacagcctga taggccgctt gggtttggat gtcgtgagaa
     9121 aatttgacgg aggcaaaaaa cttttctata tcaaaggcaa tatctttcat gaatttttgg
     9181 gcagtcgttc ctttaaggca tttgagggca aaagtcatta tgctcaaaaa tgaaatacga
     9241 gggggacttg gtattccgcc ggattgggct acaatgcgcg gacaggcaaa aaaaacgaat
     9301 gtctttgccg atgcggaaaa aagagttttc cggcgggaaa aaaggatcgt acaacatccg
     9361 cttatccgtt tcccatcagt ttgactgaat gatatcgggg ccgcgccatt attcggcagg
     9421 ccccatttta ttgccacaca ttgcctgccc gattcccgtt tcaacagcaa acccttccaa
     9481 tattttgttc aaacttggat cgcggcagat gggggcgtgc aagccccgca aggttttttt
     9541 ggcttttacg gaacagcacg catttcgcca aacggcaaaa ttgcattaac ggaattgaaa
     9601 acttttggaa ttgtgcaaaa cggcatttgc gtcagtttaa cggcatttcc aaagggcatt
     9661 ttaagccgta ttcgagagaa tgcggatggc gttttcagtg ttttcagcgt gataaaattc
     9721 aaatttctat tctaaaacaa tcagcaaagc aggatttatt tcagttgtcc gagacaacgg
     9781 gcggctgcac cgcctgcact ccccatactt tttcaacgca ctcgagcagg agtgagacgt
     9841 ggggattatc ctcgtattct tccgcataaa tgaaattgag cggcatactg tggcggcacg
     9901 attcgataat agccacgccc gcgccttctt ttgccgccgc ttcggctttg tttccctgca
     9961 ccattgccac acctataccg cctgcaacca aatcgataag ggtttggggg tagtcgcaca
    10021 atatctgttt ttttgggtga gagccggttg ctgcgccaaa actggtgcag gtgcttccta
    10081 cttccggaca cgcccgacat ttctatccat acgcattctt gcaggctctg cggaagggag
    10141 cgggtcagcc gggaatattg gctttgcggg caaatcagcg cgtaggtcag gttttgcagg
    10201 aatacgctgc ggatgccgcc ttgggcggcg ttgccgagta taaagccgcc gtgcagggtt
    10261 ttgtgttgga tgcgcgagag gatttcgccg ctcattccgt attggatgtg caggcgcgtt
    10321 ttggggggct gtttgaccga tattgtccgt cagcgcgacg agttttgccg aatcgatggg
    10381 gtggataatg cccaaactgg tctcttccga ataatcgcct gccagcgttt tggcaaaata
    10441 ctccaacttg tgtttgtatt gcagcaggga ttccgcttcg ggcaacagta tttcgcccgc
    10501 ccgcgtcaat accatgcctt tccccgtgct cctgaacagc ggcgtgccga catattcttc
    10561 aagggcttta atttgggcgg aaacggcggg ctgggaaagg aaaagtcgtt tggcggtttg
    10621 ggtaaggttg ccctcgtgcg cgacggcgac aaatgatttt aattgtacgg catccatgta
    10681 tccctccttg tgcggatgtt ttctatattt gtgcaatcga aatcttttag gcggattgtt
    10741 gctgaaaatt aactttttaa tcaagtggtt tgtaaattgt atcagttttc cggatgatga
    10801 ttattaaaaa aaaattggtt ttattgcctg tttgggcttt aaatggggtt acggcttccg
    10861 aacgcagccc gtatcaaaaa gaaaagtcat gcgccccctt ttacgaggcg cgatatataa
    10921 ggaggaaggt tatggaaaaa cataatggga cttatcggga tttacaccgt ccggcttcgg
    10981 aatttgcgac gcgggacgaa tatttggaac atgaattgca gattatgcaa ccaaaacgct
    11041 ggcggcccaa cctgcccttt cgcgattacc gcttcgagtg ggaggatttg attcctgcga
    11101 tggcgggaac gattggaaaa gtggtgatgg tgggggggcg gcggcgtttg ccgcaccttt
    11161 ggggctgcct gacagctttg tactggaaaa tgtgcgctat gagcttttaa tcgccgccgc
    11221 gtttatctta ttggtatcgg gcttttttct gcccggcgcc aacctgcccg gtacgcacgg
    11281 gccgctgatt ccgatgattc ccatcgttgt gtcggcaggc gggcatcctt tggcgttcgg
    11341 catttcgatt gcggttttag gtctgctgat ggctttattt cgcggcggca gtattatggc
    11401 gaagctgaca agcaacggcg tatgcggcgg attattactc tatttgggct ttatcggcac
    11461 gacggggcag gtaaaaaaat tgttttcgtg ggcaggcggt tttaatatgc cctacatcgc
    11521 ttttaccgtc attattgtaa cgattgtgat gtacgctttg ttggagcatt ggaaaaaacg
    11581 ctggttggcc gtgcctttgg gatgcttgat tgccggtgtg gcggcatttg cattgggtgc
    11641 gccgtttgag tttcacaccg cccccggcct gcctccaatg agtcctgctt attggtaggg
    11701 tgaaaacagc ggctggcatc tggggttgcc gacggcagaa agttttttgg ttgtctttcc
    11761 atttgcggta ttggctgttg caatgtggtc gcccgatttt ttaggacatc aagtgttcca
    11821 aaaaatcagc tatccggaaa aaaccgataa ggtattgatg aatatagacg acaccatgac
    11881 aagttgttct gtccgtcaag cagtgggttc tattttaggg ggtgcaaatt ttacctcttc
    11941 ttggggaact tatatcgtgc cggcatcgat tgccaaacgc cccattccgg gcggtgcggt
    12001 tttaacggcg gttttatgta ttatcgccgg gttgtggggc tatccgatgg acttggcgat
    12061 ttggcagccg gtattgagcg tagccttgat cgtaggcgta tacttaccgc ttttggaagc
    12121 gggcatggaa atgacgcgca aaggcaaaac cacccaatcc gccgccatcg tggtgttctc
    12181 ttccgtctgg tcaatccggt tttcggctgg gcgttgacga tgctgttgga taatttgggc
    12241 ttaatcggct gcaaagaacg cagcgcgcaa ttaggttttg tcggacgagt attgataccc
    12301 gcagtaggtt tcttaatctt gtgtgtggcg atgggtgcgg tcgggatgct gcccggtatc
    12361 cctccgtttt tggagcagtt caaatctttg ggctaggctg aaatcgcaaa tgccgtctga
    12421 accggtttca gacggcattt ttgcaaacag gcaaaatgac ggcggcggga ttttttattt
    12481 tcccgattga agtataatgt tgccgggctt caaccggata ttcaaacggt ttgctccaac
    12541 actcggaacg gcgcataaaa cgccgccctt cgcgttatcc cgaacggggc ggctaatcag
    12601 atcctatcgc cataaaaggc ggggtttcaa ccgaaaagga attgagatga ataaaacctt
    12661 gtctattttg ccggcggcaa tcttactcgg cgggtgcgcc gccggcggca acacattcgg
    12721 cagcttagac ggcggcacgg gtatgggtgg cagcatcgtc aaaatgacgg tagaaagcca
    12781 atgccgtgcg gaattggaca ggcgcagcga atggcgtttg accgcgctgg cgatgagtgc
    12841 cgaaaaacag gcggaatggg aaaacaagat ttgcggctgc gctaccgaag aagcacctaa
    12901 ccagctgacc ggcaacgatg tgatgcagat gctgaaccag tccacgcgca atcaggcact
    12961 tgccgccctg accgtcaaaa cggtttccgc ctgcttcaaa cgcctgtacc gctaaccttc
    13021 ttcagacggc atgaggcaga tgccgtctga atccgttttc ctgcaatttc gacaaacgag
    13081 aaaaatcatg agatacatca gcacgcgcgg cgaaaccgca cacaaaccgt tcagcgaagt
    13141 tttattgatg gggcttgcac ccgacggcgg actgatgctg ccggaacatt atccgcaaat
    13201 cgggcgcgaa accttggaca aatggcgcgg tttggcttat cccgaattgg cgtttgaaat
    13261 catgtgcctg ttcgttaccg atattccgga ggacgatttg cgcgacattc tgaaccgtac
    13321 ttacacggaa gcggcgttcg gtactaagga aatcaccccc gtccgcacgc tttcagacgg
    13381 catcaaaatc caagccttgt ccaacggccc gacgctggcg ttcaaagata tggcgatgca
    13441 gtttttgggc aatgcgtttg aatatgtttt aaacaaagaa ggcaaaaaac tcaatatctt
    13501 gggcgcaacc agcggcgata cgggttcggc tgcggaatat gccttgcgcg gcaaaaaagg
    13561 cgtgaacgta tttatgttgt cgcccgacgg taaaatgagc gcgttccaac gcgcgcagat
    13621 gtacagcctg caagacggga atatccacaa tatcgccgtg aaggggatgt ttgacgactg
    13681 ccaggacatt gtgaaggcag tgcagaacga tgccgcgttc aaggaaaaat accatatcgg
    13741 tacggtcaat tcgatcaact gggggcgcat cgtcgcgcaa gtggtttatt actttgcagg
    13801 ctatttcaat gccacttcaa gcaatgacga aaccgtcagc ttttgcgtac cgagcggcaa
    13861 cttcggcaac gtttgcgcgg gacacatcgc caaacaaatg ggcctgcctg tccgctgcct
    13921 gattgtcgcg accaatgaaa acgatgtgct ggacgagttt ttcaaaaccg gcgcataccg
    13981 cccgcgcaac agcgcgcata cttatgtaac ctccagcccg tctatggaca tttccaaagc
    14041 gtccaacttc gagcgtttcg tgttcgacct gatggatcgc gatcctcagg aaatcaatac
    14101 gctgtgggcg gaagtcgcgg caggcaaagg ctttgacctg cggtttgcct tggacaaagt
    14161 cggcggcaaa tacggcttta cctccggcaa atctacccac gccgaccgcc tcgccaccat
    14221 cagacaggtt tacgagcaag acaaagaact catcgacccg catactgcca acggcgtaaa
    14281 agtcgcccgc gaagtgcgcg aagcggggga aacggtggtt tgtttggaaa ccgcgttggc
    14341 ggcgaaattc gatgcgacca tacgcgaagc cgtcggcgat gtcgccattc cgcgccccgc
    14401 cgcgctggaa ggtttggaaa acctgccgca gcgcgtgcaa accgtgccga acagtgcgga
    14461 tgcggtaaaa ggcatcatcg aacaaaccct tgcctgatgt tccgtatggg cggcattcca
    14521 tccgaactta agattgaaag aaaatgacct tacctatgca ggaaacccgt ttttctatac
    14581 tgctggacga attggcggca aaacaggagg caaccatcgc cccctacctg cttgccgacg
    14641 ggaccaaggt atgggtacgc aaagcaggca ggcacaatgc gcgatggcgt tacgcgctgc
    14701 tcggtatggt tgcccgatat ttgaaactgg gggtgttgaa gccggttccc agcctcggcg
    14761 gcgagcctgc cattgcaacc gaatcaaaac gcctgtacga attgcgttcg gcagggatag
    14821 ccgttcccga attgctcgcc gtccggaaaa atgcccttat gttcggcaat ttggaaggca
    14881 tcccgctcga tacgcaaatc cgccaagaag ccgaagccgg aaaggcggac gcttggcttg
    14941 ccggtttgga agccattgca cgcgtgcata aaaaacggca gttcctcagt caggcgtttg
    15001 cgcggaatat gatgtgggac ggaaaaaaca tcagcttttt ggattttgaa gacgaccctt
    15061 ccgaggtcct gaccatagcg caatgccaag cccgcgattg gctctgctac atccattcga
    15121 ccgcgctgat attgaaaaac ggcggactgc tggaagcggc tgcggaaaaa tggggcggcg
    15181 tattgtcgga tcagcctgcc gaaatacaaa agctgattgc cggcaccgtc aaaccgatcc
    15241 tcccgatacg caggctggaa cacccccgct ggggacggga cgcgctcagg ctggcagcct
    15301 cgatttcctt aatttccctc gccgatatgc cgccgtaagc caccgcccgc cccacgatgc
    15361 cgtccgaagc ccttcagacg gcatataacc aacgcgcatc aggcattgtc gcaaccatcc
    15421 gtttcgttta gaattttgac tttatcccga atcgggcaga catttccact ctgccgcacg
    15481 tcccgaacaa gcactatcca tttcttttac tgacaggaaa cccaaatggc agcattcaat
    15541 acccaaaaag tattgtccgt acaccactgg acagacgcat attttacctt tacctgcatc
    15601 cgcgacgaat cgttgcgctt tgaaaacgga cagttcgtca tggtcgggct gatggcggac
    15661 ggcaagccgc tgatgcgcgc atacagcgtc gcctccgcca actgggaaga acacctcgaa
    15721 tttttcagca ttaaagtcca agacggcccg ctgaccagcc gcctgcaaca cctcaaagtc
    15781 ggcgacgaag tgttaatcag caaaaaaccg accggaactc tagttgcctg cgatctgaat
    15841 ccgggcaaac acctttacct gttgagtacc ggtaccggca tcgccccttt cttgagcatc
    15901 accaaagacc ccgaaattta cgaacagttt gaaaaaatca tccttgtaca cggcgtgcgc
    15961 tacaaaaagg atttggcata ctacgaccgc tttaccaaag aattgcccga acacgaatac
    16021 ctcggcgact tggttaaaga aaaactgatt tactacccga ttgtttcccg cgaagaattc
    16081 gaacaccgcg gccgcctgac cgacctgatg gtaagcggca aactgtttga agacatcggc
    16141 ctgcccaaaa tcaaccccca agacgaccgt gccatgctgt gcggcagccc tgcgatgctg
    16201 aaagacacct gcaaagtttt ggacgatttc ggtctgacag tctccccgaa aaccggcgtg
    16261 cgcggcgact acctgattga gcgcgcgttt gtggatcaat aagccgcttg aaaatgccgt
    16321 ctgaacggca tcagcgataa aaaaacccga atcctgccaa tcaggattcg gggtttttat
    16381 agtgggtaaa aattagaaat gcacatttcc gtcattccaa cacaggtggg aatccggact
    16441 tacctgtttc attatcgttt gaaaataaaa gagaaaccaa acatatagat tcccgcttgc
    16501 gtgggaatga cgacggtgtt ttgccgtagc caaagcagga aaacatcctt attgtcattt
    16561 cat
//
LOCUS       DAWTHD010000048        16536 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWTHD010000048 DAWTHD010000000
VERSION     DAWTHD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16536
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.048"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            491..2026
                     /locus_tag="WM704_001160"
     CDS             491..2026
                     /locus_tag="WM704_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9340583.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            2023..2115
                     /locus_tag="WM704_001161"
     CDS             2023..2115
                     /locus_tag="WM704_001161"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFA9340584.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(2151..2591)
                     /locus_tag="WM704_001162"
     CDS             complement(2151..2591)
                     /locus_tag="WM704_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340585.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(2785..4005)
                     /gene="lysA"
                     /locus_tag="WM704_001163"
     CDS             complement(2785..4005)
                     /gene="lysA"
                     /locus_tag="WM704_001163"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFA9340586.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(4016..4186)
                     /locus_tag="WM704_001164"
     CDS             complement(4016..4186)
                     /locus_tag="WM704_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFA9340587.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            4257..4580
                     /gene="cyaY"
                     /locus_tag="WM704_001165"
     CDS             4257..4580
                     /gene="cyaY"
                     /locus_tag="WM704_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFA9340588.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            4613..5032
                     /locus_tag="WM704_001166"
     CDS             4613..5032
                     /locus_tag="WM704_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFA9340589.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            5054..5560
                     /gene="luxS"
                     /locus_tag="WM704_001167"
     CDS             5054..5560
                     /gene="luxS"
                     /locus_tag="WM704_001167"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFA9340590.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            5704..8496
                     /gene="polA"
                     /locus_tag="WM704_001168"
     CDS             5704..8496
                     /gene="polA"
                     /locus_tag="WM704_001168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFA9340591.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            9058..9753
                     /locus_tag="WM704_001169"
     CDS             9058..9753
                     /locus_tag="WM704_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9340592.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(9745..9855)
                     /locus_tag="WM704_001170"
                     /pseudo
     CDS             complement(9745..9855)
                     /locus_tag="WM704_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9993..14399)
                     /gene="app"
                     /locus_tag="WM704_001171"
     CDS             complement(9993..14399)
                     /gene="app"
                     /locus_tag="WM704_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFA9340593.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRNATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVF
                     AVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVG
                     ILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGK
                     IRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRA
                     GIKADYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAE
                     IKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            14837..16183
                     /gene="mnmE"
                     /locus_tag="WM704_001172"
     CDS             14837..16183
                     /gene="mnmE"
                     /locus_tag="WM704_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFA9340594.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
BASE COUNT         3642 a         4219 c         4555 g         4120 t
ORIGIN      
        1 ggccgccttg tcctgatttt tgttaatccg ctatatttcc cgccatccca aaaacgaaga
       61 gcggcaggaa tttatcggaa aaacagcaac ctttccgccg tcattcccgc gaaagcggga
      121 atctaggttc gtccggtttc ggttatttcc gatagattcc tgccgcgttg ggggtctgga
      181 ttcccgcctg cgcgggaatg acgggacttt aggtttctgt ttttgttttt ctgtttttgc
      241 gggaatgacg gggaagttgg cggttgtttc ttatggagtg gtttttgtat ttggataaaa
      301 aatttttcgt tttcaagcct tcaccgcttg ccatcggcgt taaaattttt tacgataagc
      361 acatagattg taaacaatcg gccacaagcc ggtttgtttt ttcagaagac attatccctg
      421 tcagacgctg tttctatata tgtttgccta taacggcttg tttttaataa ataattcaag
      481 aggtatcaac gtgtctgatt ccaaaacgaa agaacgcgcc acattcggca cgcgccgcgc
      541 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      601 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      661 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc ggggttctgc
      721 gcccttggct ttccgccgcc tcggacgctg gttcgagccg gtcggctggt ggaacgtgat
      781 gaccaatatc gtcatctgca tctattacgc agtgattatc ggttgggcgg caagctatac
      841 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      901 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggccc
      961 tttggcgggc gtgtgggttt ttaccgccgc cattatggct ttgggcgtgc aaaagggcgt
     1021 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
     1081 catttcgctg acgctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
     1141 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
     1201 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
     1261 gggcggaacg gggctggtgg tcggttttgc caacagcagc ttcgaactgc tcgcgggcat
     1321 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggcaaggcgg tcaacgaggt
     1381 tgcctccggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcgcc
     1441 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1501 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1561 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1621 tacggcgacg gggctgcctg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1681 tgttgccgcc ggctttgttt atgttgccgc catcatcatc ggcggcaggt tgccggaatt
     1741 acgcaggcac ctgaacgcct tgtcctccat ccgcgtcggc ggcttgtgga cggcctgcgt
     1801 cgtgtttacc gtcgtgatgc tcggctatat gctgtatcag gacaccgccg gattgttgga
     1861 aaaaaactac ggcgattatc cggatggttt cctcaatatt ttcggctggg ggatgtcggc
     1921 ggcgttaatc atgttcgggc tgctgctgtc gctgctgcct tggaaacacg gtcaggattt
     1981 taatgtcaaa gacgaacacg aacatgaaca aggaggagaa aaatgagtac ttccgccatt
     2041 gtgatgatgg ttgccgccat cgcggtgatt tggggcgggc tgctgctttc cctgttaagg
     2101 ctgccggaag agtaagcctt tagaacgtta aaaatgccgt ctgaaccgct tcagacggca
     2161 tttgttttac ggctgccatt cgcgttccac cttgcccgac gcatccaaag tttgcgctcg
     2221 gacgatatgc ccgtcccccg gtacaggatg cgcgtccgca atatgccctg ttcggtatag
     2281 ccttgcgcca cgccgttttc aaccggcgtg tgcgaatgca gtttgccgtt tggatggtaa
     2341 atatcgacaa agcggtcgaa cacgccgttt ttgacgatgc ttttcaagac gcgcccgttt
     2401 gaggcgcggg tgctgaatgc gccgtccggc gtgcctgaag gcggccggct gtatgggttg
     2461 gcgggttgga tgggcgcgct tttcggcagg gtgcgaagcg gcggccggtc agagggaagg
     2521 gcggtgcagc ccgccgaaag tagggcggcg gacgaagcgg taagcaggag gataatcgga
     2581 gcgtgcttca tttcaattcc ttagatattg caccccataa aaggggcgcg taattttttt
     2641 caacaattcc cttcgtcagt cgggcgagac ggggatttgt tgcgtcgtca tttgaaaaca
     2701 ggctgtgttt gaatattccg ccaagatgtc actttaaacg gacaatgccg tctgaaggct
     2761 tcagacggca ttttgatgtt cggcttatag gcaggcgagt tcgtttgcca tttgctgttc
     2821 caaggtttcg cgccggcgga tgagtttgta tccgccgccg tcaaccaaca cctccgccgc
     2881 acggttgcgc gtgttgtaat tgctagccat actggccccg tatgcgcccg cgctgcggat
     2941 aagcagcaaa tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt
     3001 ttcacaaatc gggccgacga tgttggcggt cagaggctca atgtttttgg tttcaaccgc
     3061 ttcgatgtgg tggtaggcat cgtatagggc tgggcgcatc aaatcgttca tcgccgcatc
     3121 gaccatcaca aagtttttct cttcaccgtg tttgacaaat tcgacgcgcg tcagcaatgc
     3181 acctgcgttg ccgaccaagc tgcggcctgg ctcaagaatg agtttcagac ggcgtgtccc
     3241 catcagtttt tgaaccgctc gggcatacgc acccaaatcg gggacgcctt cgtctttgta
     3301 aacaatgccg acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag
     3361 agcgtcaacc aaaatcaaaa tgcgttcgca ggcttcgacc agtgggctta agtcggtcag
     3421 ttgcgaaccg atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg
     3481 gcggtaggct tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt
     3541 ggagatgtag ggatgggttt ttgcatcgac atcgggattg acgcgcaggg agacgggcgc
     3601 ggttttgccc aaacgcgcgg caattttctg aatgcggtcg atttcgggga tgctttccat
     3661 attgaagcat tttacgccgg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac
     3721 gccggaaaaa atcgttttcg ccgcatcgcc gcctgccgcc aaaacgcgtg ccaattcgcc
     3781 gcccgacaca atgtcaaaac cgctgcccaa agaagcaaag tgtttgataa tgctcaggtt
     3841 gccgtttgcc ttgacggcgt agcagacaag cgggttcaaa gcggcaaagg cggtttgata
     3901 gttttcaaat gctccggtca gcgcggattg gctgtacaca taaagcggcg tgccgaattc
     3961 ttcggcaagg cgggggtagg cgacttgttc gcaaaacagg gtcatatttt cgttttcatt
     4021 tttgggttgg tggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattgcgc
     4081 cgaaacgcgc cgtatcgcct tctttgggca ggtagaggcc gcctttgtaa ccgcaggcgg
     4141 aaagcaggag ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt
     4201 cataagcgcg aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga
     4261 tgaccgaaag cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa
     4321 tcgacgaaaa cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag
     4381 ccggagacgg cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttgtggattg
     4441 ccgcaaaaag cggcggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg
     4501 acagccgcga tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg
     4561 ttgagattgc cgaattgtga ttcaattttc gatataaaaa gattgttacc caatggcaca
     4621 attacctcta tatcggactg ccgaaatcgg aaattttact gtcggcacgc ctaaagtttt
     4681 agaatcattt tccaaacata tcccttatgg tgttgtcttt gaagatgatg gcaacacggg
     4741 ctacttctat gccgcttcgc aagaggggat tttggatgcc ttgcacatct acaatgtcga
     4801 agatgtatcc gacaaacata tccccaatca tgtcttgatt ttatgggatg gcgcctgcac
     4861 catagccgca ttgtgtatca acgactacat ccatgccgtc tatgattttg tcgaacaggc
     4921 aggatattgc cgcaacggct tccctgaagc aggcggcgaa tgggtaaaag tcgaaaaccg
     4981 cgtcttggac gatgaattgc tggacaaaat cctatcccga aaacctacat aacccgcaca
     5041 aaaggatacc caaatgcccc tgttagacag tttcaaagtc gatcacaccc gtatgcacgc
     5101 ccctgccgta cgcgtggcga aaaccatgac cacgcccaaa ggcgacacca ttactgtatt
     5161 cgacctgcgc ttttgcatcc ccaataaaga aatcctgccc gaaaaaggca tccacacgct
     5221 ggagcatttg tttgccggct ttatgcgcga ccacttgaac ggcgccggcg tggaaatcat
     5281 cgacatttcc ccgatgggct gccgcaccgg tttttatatg agccttatcg gcacgccttc
     5341 cgaacagcag gttgccgatg cgtggttggc ttcgatgcag gatgttttga atgtcaaaga
     5401 ccaaagtaaa atccccgagt tgaacgaata ccaatgcggc acttatctga tgcactcgct
     5461 cgccgaagcg caacaaatcg cgcaaaacgt gttggcgcgc aaagtggcgg ttaaccgaaa
     5521 cggcgatttg gctttggatg aaagcctgct gaacgcttaa tccgccgcaa atgctgtctg
     5581 aacaagggtt tcagacggca tttgcctttt ccgttataat ccggggttgt acgggggcgg
     5641 gttttaagcc ggcatcgtcc ttccctattt ttctgtccct tatcggtttc aagcgggttt
     5701 tttatgtcca acagacctac actcctcctc gttgacggct cttcctatct ctaccgcgcg
     5761 tatcacgcga tggggcaaaa cctgactgcg cctgacggtg cgccgacggg tgcgctgtat
     5821 ggcgtattga atatgttgcg ccgtttgcgg tcggaatatc cgcacgatta ttgcgcggtg
     5881 gtttttgatg cgaaaggcaa aaatttccgc catcaaatgt ttgaagaata caaggcgacg
     5941 cgcccgccga tgccggacga tttgcgcccg caggcggaag ccctgccgga tttggtgcgc
     6001 ctgacgggct ggccggtatt ggtgattggg caagtggagg cggacgatgt gatcggcacg
     6061 ctggcgaaac agggggcgga acatggtttg cgagtcattg tttcgacggg cgataaggat
     6121 atggcgcagt tggtggatga gcgcgttacg ctggtgaaca cgatgagcgg agaaacgctg
     6181 gatattgaag gcgtgaaggc aaaattcggc gttcgccccg accaaatccg cgattatctc
     6241 gcgctgatcg gcgacaaggt ggacaacgta ccgggcgtgg aaaaatgcgg cccgaaaacg
     6301 gcggtgaaat ggctggaagc ctacggttcg ttgcaaggcg tgatggaaca cgctccggaa
     6361 atcaaaggca aggtcggcga aaacctgcaa gccgcgctgc cccaactgcc gctgtcgtat
     6421 gatttggtca cgattaaaac cgatgtggac ttgcacaccg agctttcaga cggcatcgaa
     6481 agcctgcgcc gtaccgcgcc gaaatgggcg cagcttgcgg ttgatttcaa acgctggggc
     6541 ttccgtactt ggctgaaaga agcggaatca aacatgaata ccggctcgac cgatgatttg
     6601 ttcggtagcg acagcatcgg cgagcaggcg gctttgaatg cggaaatacc gtctgaaaaa
     6661 cgagctgaaa aagccaccgc ccctgaaaaa ctggattatc aagccgttac caccgaagct
     6721 cagtttgccg ccttgttgga caaactagcg aaggcggaca caatcggcat tgatacggaa
     6781 accacgtcat tagatgcgat gaatgccgag ctggtcggca tcagcattgc gttccaagca
     6841 ggcgaagcgg tttacatccc cgtaggccac agcctgactg ccgcgcccga acagcttgat
     6901 ttgcaagacg tattaggccg tctgaagccg catttggaaa accccgccct gaaaaaaatc
     6961 ggtcaaaacc tcaaatacga ccaacacgtt ttcgccaact acggcatcgc gttaaacaac
     7021 attgccggcg acgcgatgct cgcttcctac atcatcgaga gccatctcgg acacggcttg
     7081 gacgaattgt ccggacgctg gctcggttta gaaaccatta cctacgaatc gctgtgcggc
     7141 aaaggcgcga agcaaatcag ttttgccgat gtcgccatcg ggcaggcgac cgaatacgcc
     7201 gcccaagatg ccgatttcgc cctgcgcctc gaagcgcgcc tgcgcgcgca aatggacgac
     7261 aaacaacttg aaatgtatga aaaaatggag cttcccgtcg cgcaggtatt gtttgaaatg
     7321 gaatgcaacg gcgtgcaaat cgaccgcgcc gaactcgccc gccaaagcgc ggaactcggc
     7381 gcggagctga tgaagctcga acaggaagcc tacgccgctg caggtcaacc gtttaacctc
     7441 aactcgccca aacagctgca agaaatcctg ttcgacaaaa tgggcatccc gacaaaaggg
     7501 ctgaaaaaaa ccgccaaagg cggcatttcc accaacgaag ccgtgctcga acagctcgcg
     7561 cccgactacc ccctgcccaa aatcatcctg caaaaccgca gcctggcgaa actcaaatcc
     7621 acctacaccg acaaactgcc cgaaatgatt tcgccccggg ataaccgcgt gcataccacc
     7681 tatgcccaag ccgtcgccat taccggccgc ctcgccagca acaaccccaa cctgcaaaac
     7741 atccccattc gcaccgccga aggccgccgc gtgcgccgcg ccttcactgc accgccgggc
     7801 agcgtcatcg tttccgccga ctattcccaa atcgagctgc gcattatggc gcacctctcc
     7861 ggtgacaaaa ccctcattgc cgcatttcaa agcggcgaag acgtacaccg ccgcaccgcc
     7921 gccgaagtgt tcggcactgc gcccgaaaac gtctcgcccg agcaacgccg ctacgccaaa
     7981 accatcaact tcggcttaat ctacggtatg gggcaatacg gtttggcaaa atcattgggc
     8041 atcgacaacc tttccgccaa aaactttatc gaccgctact tcgcccgtta tcccggcgtc
     8101 gccgaataca tgcagcgcac caaagaacaa gccgccgccc aaggctacgt cgaaaccctg
     8161 ttcggcagaa ggctctattt gcccgacatc cgcaacaaaa acgccaacgc ccgcgcagga
     8221 gccgagcgtg ccgccatcaa cgcccccatg caaggcaccg cttccgacct catcaaacgc
     8281 gccatgataa acgtgcgaaa ctggctttca gacggcatcg gcagcaaact ggtcatgcag
     8341 gtgcatgacg aactggtgct ggaagtcgtt gaaaccgaac tggattttgt caaagaaaaa
     8401 ctgccgcaga ttatggcgaa agtggacggc ggattattgg atgtaccgct ggtggctgag
     8461 gttggcgtag gggagaattg ggaagaggca cattgagaat gcacaagtat tgaactgtta
     8521 cgttttgatt tatcaaaatt taaggaagta aaatacccga ttcgaatgat tggaaatcaa
     8581 tagcaagccg taggtcggat acttgtatcc gacaaaagcc tgccatctca aatagcagtc
     8641 ggattcgaga atccgacctg ccaaaccggg cgcggacgct ccggtcggca gttagcgcac
     8701 aaatcgaaca gaacatcaca aaaaagcctg attcggattt tccaatcggg cttttttgcg
     8761 cccgttttgt catcccgtga aatatccgca taacaaaaat aatgcaaaat catgacaaaa
     8821 ccggcgcgag gttacacaaa cggatgaaat caaccgatat tcaaacacag ccatttttag
     8881 cgcattttca gcgtatcttt atgcggaaaa tttcgtgaac aggttttttg cacaggcttc
     8941 cgccgcgttt cgcgggatgg gaaaccgtat tagaaaacgg acgcacgcaa cataaacccc
     9001 gaaagtgatg ataagatgat gatttaacgt actgctttaa ttatttaagg aattatcgtg
     9061 tttcccgaca aatacaagtt gagtttggaa gaaaatattt ttctggcaaa gaaagtattg
     9121 gttgctcaaa ttcacaacct cagccgtttt gagaattgtc agacgacctt gttgcagacc
     9181 gagcaaatta tcaatggcaa aaatgtagcc tccgcgtcac tggaagacat ccaaaccatc
     9241 ttgaacctga aacgtgccta tcaatatgtg atttcgcata tttcaaacgg cgaaccggtc
     9301 aatatttcac tccttaaaag aatcaacaac attgttgcca aggacgattc tttggtaccc
     9361 gtggatttcc gtaccggttc ggtcggcgta acgctattgg acggttcccg tcatgccccg
     9421 aatccggtga aggaaattga agtggcccgc gtgttgcaaa atatcggact gcaaagcggt
     9481 tcgacgacgg aagcagccgt tcgtttcatg ctctattgta tgcggcagca ggttttttgg
     9541 gacggcaaca aacggacggc aaccctattt gccaacggcc tgatgatggc ggggggctgc
     9601 ggtatcctgg aaatctccga aatgcagatg ccgcgattca atgaaaaact atccgcattc
     9661 tatcgcaccg gcgacgatac cgatatttcc aagtttgtgt atcaaaattg tatatcgggc
     9721 atagactatt tcggcgcgga cgaagatata tagtggatta aatttaaacc ggtacggcgt
     9781 tgcctcgcct tgccgtacta tttttactgt ctgcggcttc gccgccttgt cctgatttaa
     9841 atttaatcca ctatagattt ccccgactcg ccgaacatgg gctaaaaatc aatttgacgg
     9901 ttatcagacg atggagtagg cacaaggtgg cagaaaaagg ggtttgacag tgcacggcgg
     9961 catcgtcaaa cccctttcgg catatccggc ggttaccagc ggtagcctaa tttgatgccc
    10021 gcgctgtgct gcgcttccaa ttgcggcccc ttggcggcgg cagcgtggag ggacagcgtg
    10081 aaacctttga tttcggcgtt tacgccccat tccgcactgc gggttttgcc gaaatcctgc
    10141 gccaatacgg cggtattgac gcgcgttcgg actttgccgg aagcggcatc ggtataggac
    10201 aggctcaaat aaggcgtgat ggaaatgtgt tgcgccggtt tgaatgaata atctgcctta
    10261 atgcccgcgc ggtagcggtt gaatgcaagg cccggggtgg cgatattgac gttttcgtat
    10321 cggtaatccg ctttttggac gaaatagcgc gttgcgccga tgtgcggttc gatgccgaat
    10381 ccgccgaaac ctgcgcggta tcttgcctga atgccgtaat gcagcacgcg gcggcggatt
    10441 ttgcctctga tgccgtctga aaggctgccg ctactaaaac ccgcgcccgc gctgatgccg
    10501 atgtcgaacc tgccgatgcc gtattgcccg aaaacggcac cgtgggcaag ccgtgccgag
    10561 ttgccgatgc cgtcgtcgaa ggtgttttcg gtccggttgt gcgaaaacag gatgccgacg
    10621 cgcccgctgc cgaggttttt ctgcataccg atttggcgca ggtcggtttg ttggcggtag
    10681 gcgcggaaat cttgcgaacg gtagtgtttg gtgtcccgga tgccgcttgt ccaaacggcg
    10741 ttgcggcggt cttcggcaaa cacgcggtcc aattcgtcct gtacggcgaa aacgctgttg
    10801 agcgtggcgg aaaattcact caaaccgcta ttggcataac ggctgatcag gtcgcgctgc
    10861 ggttggggtt gcggttgggg ctgcggttgc ggcaaatccc ggcgggcgcg gcgggcgcgg
    10921 gggaaggcgg tggtagccgg ccgggtttcc gcttcgcgct gtttcgccaa ggcggtgtct
    10981 ttatccgcct gcacccgttt tttctcttcc tccgcctgca taatgccggc attttccccg
    11041 cctgcctgcc gggccggttc ggcaacgctt tctgcctttt cggtggcatt gcgcccggcc
    11101 gcaatcagcg cgtcaaggct ttgcgcgttg tctttttccg cctgttgttt ggcggcaagt
    11161 tgtgcctgtt ttgccgtcaa ggcggcctct gtttctcccg ccttgccgag tttgtcggaa
    11221 agctcttgtt ctttgaccgg attatgcagg cggaactcgc cgtctttgcg gataagctga
    11281 taacgccatg cgccggcatc gacgtgttcg ttttgcaggg tgaaattaag attttcggac
    11341 agcggtgtgt tgtcttttcc ttccactacc gtcaattgct cgagacttac gggttcgttg
    11401 ccggtattgt tgacagccaa ggtgtaagtg ccttcggaac tttccgccag cttcaatttg
    11461 ccgctgcggt agccgaagag ttccgacata aagcggaatg ttccctgacc gttcaatttg
    11521 ccgtttaccg tcagcgtgtt gaaacgggat tctgccgaag ttggcggcgt aacggataat
    11581 agggaacggc gcgaacggcg gcgcggcgca tctgccgcac tgccggtttg cgcgcctgcc
    11641 gcatcgtgtc gataggcgga attgagtgta atggtggcgt tgtcaaggtt taaattgcct
    11701 aattccgtgc ccgacggcag cgtccattcg ctgtctttta agtgtaatgc cgtatccttg
    11761 ccgccgctga tttttccggt aaagcggctg ttttcaaaat ggaatactgc cttatcggct
    11821 agggagacat tgccgttgag tgcggaatgg cttacgtttg ccttagcgtt gtcggaaagc
    11881 gtcagactgc cgttttgtac ggcgttgttg cttagattaa atgaagcatt gtccgaagcc
    11941 gatgtgttgc cgtttaatgt ggcttgatta aatgttgctt gggcattgcc cacgaggcta
    12001 aggttgccgt tttgggtggc gttgcgcgta accgtatagt gcgtgtctcc gcctgcacta
    12061 agattgccgt tgagtgtggc aagtcctgtg agatttaaat gagcgtgatc ggcaaggctg
    12121 acattgcctc tgatgtcggt cttgctcaat gaagcaatca ctttatcgtc ggtaatggtt
    12181 ttttcggtac aacttgtcag acccgtccag tccgaacgtg tacagattgt gtggctttga
    12241 tgcggtgcga caccgaaaac tgcttgggcg tgattgctta aatgccaatc gccttccact
    12301 ttggcaacat tgcgggaaac caccgcttgt ccgccctgaa tatggaagtt ttccgcttta
    12361 aatgtgcggt cgatccaatc gttgtcccac acgatttctc cttgtgggat accttccatt
    12421 tttgaccacc cgcttcctaa atgattgtag gcgtgcggtg tcggtctgcc gctgaaaaac
    12481 agtttgccgt ttgtttgcgt gatattgccg tttaaatttg ttccgccgga aagcagtaaa
    12541 gtgcgatccg cttcttccgg ttggtaattc agattgagcc gcccgttcgt tttggttgca
    12601 tctttctcgc caaaccaacc gttgtaggca atttcttttt tgctatccaa gttgttgtta
    12661 ttgccggttg tagtaatatc tttattgcct gtaatggtaa cggtggattc tttgtcttga
    12721 ttgtggttga caatcatcgc cccttcatcg gtattttgaa tgcggtggaa cgaaagcgaa
    12781 tgcccgttca aatccaaacg tccgccgcga aagccgaaat agagtttgtc ggggttgaac
    12841 tgattatcgg cattcagttg caccgtcccc ctgccgctga ccaagccgat ttcactaaag
    12901 gcttgttttt tgccttgatc gtccgcctgc tgatctaaga tgactttacc gtcgcccacg
    12961 ctgaccgagc cttggttttc ccctttggct tgaaccagca gcgtgccttt gccgattttg
    13021 gacaggcggt cgtttgccac gccgtttact ttccaagtaa cggtactgcc atcactgata
    13081 tgaacgcccg cgccttgcca cgtttcgttg tttttaggcg agaccgtaaa attaccctca
    13141 aaatacaaac cgcccgcgcc ttggttgatg ttgctggtaa gtatcaattc accttttcct
    13201 ttgtcaataa aggaaatatt ttctccatta ttcagtctgg gtcgataact gttgacccca
    13261 cctgcagcat gataaacagg ttctcttgct gtctcggata aagaaacatt aaacaattga
    13321 acggttcgtg tttttaatct ataaggtaga gaatagtgtt tatgtttggc atcgattttt
    13381 cctgcgccat tattattgtc gttaaaaaag tatttcccat tttgatgtgg ttcgtagaat
    13441 actgaatggg tatctccagc aaagatttca tcatagaacc aatctttacg aactagctgg
    13501 aagccattgc tttttcctat ataggggttg cctgtttgca ataccccatt aattaaccac
    13561 ttttgctttt gggcatcata gataaacatt ggtgagccac tgtcgccaaa tgagcctcct
    13621 gttggtaaaa aaccatatgg gctatgttta attttttcgc tacctaagtt gactgtgcca
    13681 ccacctgatc cattttgtgc aaaggtattg ccaccgacga gccaagaata tgcgcttgca
    13741 atatgatatg aactttcgcg gttattgggt tcgtcttcat cagaccgcca atattgtctg
    13801 cctgctccga ttcgaacacg atcagggtat ttatttaaat cagcgtattt ccacccatcc
    13861 atataactgg tcatctcaac aggttctgca tctgtgacaa atttgtgcaa acgcggcata
    13921 tgataatcgc cgccataagg atggccgtta gtccctgctt tataattatt tcttttcaca
    13981 atttggtaag aaaagcggtg ctgatcggga ttgcttccct ccgcaccaaa atcaacattg
    14041 ttatagccgc cgttatgtgc cacgctcaca atatattgat cgcccgccaa tgccgccacg
    14101 ccgttacgcg ataccacaga aaaatcaatc atcggggctt tcgtcatcga tttgccgacc
    14161 aactcccctt ttttgttgta aacctcaata tctttcgccc cgactgcaaa cttgccttta
    14221 ttttcggcaa agtcgcgata gtattggtag ttgatgccga aataagtgtg tcccgcccgg
    14281 gcttggggca gaatgccgaa cgacaggcat atggctaagt aagcgggcga gaagcggatg
    14341 cggccggttt taggggcttt gcggtgtgtt tcggttgtcc gtttgtcggt tgttttcatt
    14401 atttttcctt atctgacggg attcggtttg tttgggaggg cgcggcttcc gcttccgggc
    14461 ggcgcgcggg atgtggctat atgtgcggtt cggcgtattt tgccgcttct gttcggagat
    14521 ggtgcaggga agggcgggaa ggaaaaaaag gggattttta tcaactcgcc ggagcaatcg
    14581 atgggttgca cataaaggca tcattttgaa tacgaaatca tatcacactt tactttatcc
    14641 ccgttccttt tcggtcggaa ccccgccgtt ttcggcggta aaaaccgacc ggaggcggaa
    14701 tgcggggcag ggttttgtgc cgtgcggtgt cggaatatcg ttttacacaa gccggcaatg
    14761 ccatctgaaa gccgaaccgt cggacttcag acggcatttg ctataatcgc ggctgttttg
    14821 aattttcggg ggttttatgt cggataacgt tccaacgatt gcggcagtcg cgaccgcgcc
    14881 cggacgcggc ggcgtgggcg tgatacgcat atcggggaaa aacctgttgc cgatggcgca
    14941 ggctttgtgt gggaaaacgc ccgagccgcg cgtggcaacc tatgctgatt ttacggacgc
    15001 ggacggacag gcaatcgata gcgggctgct actgtttttt gccgcgccgg caagttttac
    15061 gggcgaagat gtcatcgagc ttcagggaca cggcgggccg gtagtgatgg agatgctgct
    15121 gaaccgctgt ttggaactgg gcgcgcgcct tgccgagccg ggcgagttta ccaaacgtgc
    15181 gtttttaaac gacaaactgg acttggcaca ggcggaaggc gtggcggatt tgattgacgc
    15241 atccggccgt tcggcggcgc gtctggcgtt gcgctcgctc aagggcgatt tttcgcggcg
    15301 gatacacggt ttggtcgaag gcttgattac cttgcggatg ctggtcgaag cggcgctgga
    15361 ttttcccgag gaagacatcg attttctcga agcggcggac gcacgcggca aactggacgg
    15421 cttgcgccgc gccgtggatg atgtgcttgc caacgcgcag cagggcgcga ttttacgcga
    15481 aggtctgaat gtcgtattgg tcggtgcgcc gaatgtcggc aagtccagcc tgttgaacgc
    15541 gctggcgggc gacgaagtgg cgattgttac cgatattgcc ggaacgacgc gcgacgcggt
    15601 cagggaacgt atcctgattg acggcgtgcc ggtgcatatt gtcgatacgg caggtttgcg
    15661 cgagacggac gacgtggtcg aacgcatcgg catcgaacgc agccgcaaag ccgtatccga
    15721 agccgatgtc gcgctggtgt tggtcgatcc gcgcgagggt ttgaatgaaa agacgcggat
    15781 gattttggat acgttgccgt cggatttgaa acgcattgaa atccacagca aatccgattt
    15841 gcacgcacac gcggcaggcg ggttcggtac gggcgcggaa accgtcatcg cgctgtcggc
    15901 gaaaaccggc gacggcttgg acgcgctgaa acggacgctg ttgtgcgagg ccggttggca
    15961 gggcgaaagc gaagggctgt ttttggcgcg gacgcggcac gtcaacgcac tcaaggcggc
    16021 gcaggaagaa ttgtcgctgg ccgctttgtg cggcaaccat caaatcgagc tgtttgccga
    16081 acacttgcgg ctggctcagg tcgcgtgcgg cgaaattacg ggggaattta cggcggacga
    16141 cctgctcggc gtgatttttt cgaggttttg catcggaaaa taagcgggcc ggaggcattg
    16201 tggcggtgtc cggctgaaca ttccgttatc ccataaaaac gggaatccga tccgttcggt
    16261 tttggttttt tgaatttcgg gcaacgctta aatcttcatt ccgcgcaggc ggaaattatc
    16321 ggcgcggtac ggcaactttt ttcaatactg aaagccccgt cattcctgta aaaacaaaaa
    16381 aatcaaaaac agaaaactga aatatcgtca ttcccgcgca ggcgggaatc cagccccgtc
    16441 ggcacggaaa cttatcggga aaacggtttc tttagatttt acgttccgga ttcccgcttt
    16501 cgcgggaatg acgggattaa agtttcaaaa tttatt
//
LOCUS       DAWTHD010000049        16520 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWTHD010000049 DAWTHD010000000
VERSION     DAWTHD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.049"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(180..1109)
                     /locus_tag="WM704_001173"
     CDS             complement(180..1109)
                     /locus_tag="WM704_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFA9340595.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1238..1987
                     /locus_tag="WM704_001174"
     CDS             1238..1987
                     /locus_tag="WM704_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFA9340596.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2110..2691
                     /gene="petA"
                     /locus_tag="WM704_001175"
     CDS             2110..2691
                     /gene="petA"
                     /locus_tag="WM704_001175"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFA9340597.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2710..4059
                     /locus_tag="WM704_001176"
     CDS             2710..4059
                     /locus_tag="WM704_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFA9340598.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4074..4862
                     /locus_tag="WM704_001177"
     CDS             4074..4862
                     /locus_tag="WM704_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFA9340599.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDTYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLLAVMLPLAYFLKKEYWKDVH"
     gene            5194..6984
                     /gene="rmuC"
                     /locus_tag="WM704_001178"
     CDS             5194..6984
                     /gene="rmuC"
                     /locus_tag="WM704_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFA9340600.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(7051..7683)
                     /locus_tag="WM704_001179"
     CDS             complement(7051..7683)
                     /locus_tag="WM704_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFA9340601.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7776..8600
                     /locus_tag="WM704_001180"
     CDS             7776..8600
                     /locus_tag="WM704_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFA9340602.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8669..9232
                     /locus_tag="WM704_001181"
     CDS             8669..9232
                     /locus_tag="WM704_001181"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFA9340603.1"
                     /translation="MTDLETKRLETQAMLENAELLFDQGQCRAALQKVAYEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            9316..9753
                     /locus_tag="WM704_001182"
     CDS             9316..9753
                     /locus_tag="WM704_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFA9340604.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9974..10243
                     /locus_tag="WM704_001183"
     CDS             9974..10243
                     /locus_tag="WM704_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFA9340605.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            10243..12018
                     /gene="ptsP"
                     /locus_tag="WM704_001184"
     CDS             10243..12018
                     /gene="ptsP"
                     /locus_tag="WM704_001184"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFA9340606.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(12145..13086)
                     /locus_tag="WM704_001185"
     CDS             complement(12145..13086)
                     /locus_tag="WM704_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9340607.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(13095..13970)
                     /locus_tag="WM704_001186"
     CDS             complement(13095..13970)
                     /locus_tag="WM704_001186"
                     /inference="COORDINATES: protein motif:HMM:NF012514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFA9340608.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(14153..14229)
                     /locus_tag="WM704_001187"
     tRNA            complement(14153..14229)
                     /locus_tag="WM704_001187"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14193..14195),aa:Met,seq:cat)
     regulatory      14378..14477
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14568..16469
                     /gene="thiC"
                     /locus_tag="WM704_001188"
     CDS             14568..16469
                     /gene="thiC"
                     /locus_tag="WM704_001188"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFA9340609.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYQNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIECAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLHYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         3959 a         4352 c         4615 g         3594 t
ORIGIN      
        1 gtctaaaacg tcctgttgat ccatatattt cacccgttat gagtctgttc ccatctcata
       61 tagtttcaag tttaacaacc ccttgaggct atgttaaaag agtttttcta ataactattc
      121 cgttttctta aatataaaaa aatagaaaaa tagcccgggt atagtttgaa caagggaaat
      181 cagaccggtt ccaactcact taatccgggc aagtccgcga agccgcgttc acgtatgatt
      241 tggcaaaagt tgttcagata acttttatct gtgtcttcag tgcggatggc ggcatacagt
      301 ttgctttgca gtccgtcggc agtaatttgg cggtggacga catagccttt ttcaaggtag
      361 ggcatgactg tccaataggg aagggcggca atgccacgtc tgctggcaac cagttggata
      421 atggcgatgg tcagctcgct gtgccggcgc ggcgggttga tgtttttcgg aatcaggatt
      481 tttttgggta aatccagcat ttcatcggga acgggatagg taatcagggt ttccccgata
      541 aagtcttccg ccgtccaaac gtttttggcg gcaagcggat ggtccggggc gcaaatgccg
      601 accatttcgt aggcaaacag tgggtggaag ctgatgctgc tttgtttttc ggcttcggag
      661 acaatggcga ggtcggcgcg gtgttgcagc agcagcccga cgggatccgc ttggaatcct
      721 gatacgatat ccagttcgac ttggggccac atcgggcgga attcgcccat ggcgggcatc
      781 agccagtcga aacaggtatg gcattcgacg gcaatccgca gctctcccgc ctccccttcc
      841 gtgattcgcg ccaaatctcg ttctgcaaca gcaacttgag gtatcagttc gtgggcgagg
      901 cgcagcagcc tttcgcccac cggggtaaag cgcaggggcg tggatttgcg ttcgaacagc
      961 ggcgtgccgt agtggttttc gagcatacgg atctggtggg aaagggcgga ttgggtaagg
     1021 aaaacccgtt tggcggcaag ggagacgctg ccggtttctt caagtgccag cagggttttg
     1081 aggtggcgca attcgataat ggaatccatg gggcttcaga cggcatattg aaccggcgca
     1141 tattaaaata attcatatgc gggtgcaaat cggaaaaatg ggcgtttcgg gcatttgcgg
     1201 ttagaatgcc cgcttgtttg gaaatgacag gaaaaatatg gttttgcgca gagatttttt
     1261 agcctggtgc gacgaaacgt tgcagacggc atcgtttaaa gattacgccc ccaatggttt
     1321 gcaggttgaa gggagggaat atatcgggaa aatcgttacg tcggtaacgg caagcagggc
     1381 agcgattgat tttgctgtgg agcagaaggc agatttgctt ttggtacatc acggtatgtt
     1441 ctggaaaaac gagctgccga ccgttaccgg ttggaaaaaa gaacggattg ccgcactgtt
     1501 acggcacgac atcaatatgg caggctacca tctgcccctg gatgtgcatc ccatactggg
     1561 caacaatgcc caactcgccg acagattggg ttttgcgaca gaaaaacggt tcggcgaaca
     1621 aaacctgctc aactcgggca gcctgaaaca agccaagaca ctcggcgcat tggcggcgca
     1681 tattgaaaca gttttgcaac gtaagcctgt cgccatcggc aatcccgaac gcgaaatccg
     1741 acgggttgca tggtgtaccg gcggtgcaca gggctttttt cagacggcaa tagatgaagg
     1801 cgtcgatctg tatctgacgg gtgaaatttc cgaagcccaa taccacctcg ccaatgaaac
     1861 gggtacggct ttcatttcgg cagggcatca cgcgacggaa cgttacggcg tacgcgcgct
     1921 ggcagaatcg gcggcagagg ttttcgggtt ggaagtgtgc cattttgacg aaaacaaccc
     1981 ggcttgagtc ttgagaaata tcataaaact ttaccttatt ttaaataatg ctttgagtat
     2041 cactgcaaac tgggtaaaat tgcaatgttt aatggctcgg cattcccaaa atattaggac
     2101 gactgaataa tggataatca agaaatcaac aacggccgcc gccgtttcct gacacttgcg
     2161 acctgcggcg cgggcggagt ggcagcattg ggtgtggcaa cgccgtttgt ggccagtttt
     2221 ttcccttcgg aaaaagccaa ggccgccggt gctgccgtcg aggtggatgt cagtaaaatc
     2281 gaagcgggtc agctgctgac cgccgagtgg caaggcaaac cgatttgggt gctcaaccgt
     2341 acagatcagc agcttaaaga cctgaaaggc ctgaacggcg aacttaccga tcccaattcc
     2401 gatgcggaac agcagccgga gtatgctaaa aacgagaccc gttcgattaa gccgaacatc
     2461 cttgtcgcca tcggtatctg cacccatttg ggctgctcgc ccaccttccg tcccgacatt
     2521 gcccccgccg atttgggcgc agactggaag ggcggcttct tctgcccgtg ccacggttcg
     2581 aaattcgact tggccggccg cgtatataaa ggtgttcctg ccccgaccaa cctggttgtc
     2641 ccgccatata aatacttgag cgacacaact atcttggtgg gcgaagacta agaataagga
     2701 acgataatta tggcaaacca aaccaatagc aaagcaaaag cattgttagg ctggatggac
     2761 gctcgcttcc ccctgtctaa aatgtggaat gagcatttgg cgcaatacta tgcgccgaaa
     2821 aactttaatt tttggtacta tttcggctct ttagccctgc tcgtcctcgt gattcaaatc
     2881 gtcagcggta ttttcctgac gatgaactac aaaccggacg gcaacctgaa tgcctaccat
     2941 ctgcctgctg cctttaccgc agtagagtac atcatgcgcg acgtgtccgg cggttggatt
     3001 atccgctaca tgcactccac cggcgcatct ttcttcttca ttgttgttta tctgcacatg
     3061 ttccgtggtt tgatttacgg ttcatataaa aaaccgcgcg aattggtgtg gattttcggt
     3121 tctttgatct tcttggcatt gatggcggaa gcctttatgg gctacctgct gccttggggt
     3181 cagatgtcct tttggggtgc gcaggtgatt atcaacctgt tctccgccat tcccgtcatc
     3241 ggtcctgatt tgtccacttg gattcgcggc gacttcaacg tttccgacgt taccctgaac
     3301 cgcttcttcg ccctgcacgt tatcgccgta ccgctggtat tgctcggttt agtcgtggca
     3361 cacatcatcg cactgcacga agtgggctct aacaaccccg acggtgtgga aatcaagaaa
     3421 aacaaagatg aaaacggcat tccgcgcgac ggcatcccgt tccaccctta ctacactgtt
     3481 aaagacatcc tgggcgtcgt tgtattcctg attgtcttct gtgccgtatt gttctttgcc
     3541 cctgaaggcg gcggctactt cctcgaagcg ccgaacttcg atgcggcaaa tgcgctgaaa
     3601 acacccccgc acattgcgcc ggtatggtat ttcacaccgt tctacgccat tttgcgtgcg
     3661 attccttcct tcttgggaac gcaggtttgg ggtgtaatcg gtatgggcgc ggcggtcgtg
     3721 ttgattgccc tgctgccttg gctggacagg ggcgaggtga aatccgtccg ttaccgcggt
     3781 ccgattttca aaaccgcatt ggtcttgttc atcatttcct ttatcggttt gggtattttg
     3841 ggcgcgatgg tggcaaccga tacgcgtact ttggtcgcgc gcatcctgtc cttcgtctat
     3901 ttcgcattct tcctgggtat gccgttctat accaagctgg ataaaaacaa accggttccc
     3961 gaacgcgtaa ccatgagcac cgccaaacaa aaaatcatgt tctttgttta cgtcggcatt
     4021 actgccgtgg gtgcttacct gtttgcaacc aacatctgat gagggcagtc gaaatgaaac
     4081 aagctatgaa gaattggttt gctgccttgc tgtcggcagt acccatgagt gcggcatttg
     4141 cttcgggcgg tcatgcacac tatgagaaag tcgatattga tttgcgcgac caagtaagct
     4201 tgcagcgcgg cgcacaaatc tttaccaact actgtttgtc ctgccactcg gcaagcggta
     4261 tgcgtttcaa ccgtttgaaa gacatcggtc tgactgatga agaaatcaag aaaaacctga
     4321 tgtttaccac cgataatgtc ggcgatgtca tgcattcggc gatgaacccg aaagatgcgg
     4381 caaaatggtt tggtgctgct ccgcccgatt tgacattgat tgcgcgttcc aaaggtgcag
     4441 actaccttta cgcctatatg cgcggcttct ataaagatcc gacccgtccg agcggctgga
     4501 acaataccgt attcgataaa gtcggtatgc cccacccgtt gtgggaacag caaggtgttc
     4561 aggcagtcga gttggatgcc aaaggtcagc cggttatggt aaaagacgaa cacggcgaga
     4621 tgaagcctaa gctgtattgg gaatctaccg gcctgcacag ccgccgcctg ccgaacggca
     4681 aagtcatcca aaaagagtac gatacttatg tacgcgattt ggtcaattac cttgtgtaca
     4741 tgggtgaacc tgcacaactg caacgcaaac gtataggcta tgtcgtgatg attttcttgt
     4801 tggcggttat gctgcctttg gcatacttcc tgaaaaaaga atattggaaa gacgtacact
     4861 aagcgtttgg aacaaaaggg caaatccttt agggtttgcc ctttttgcat gcttctgccc
     4921 tttgggaaaa tcagcacccg gatgccgtct gaacatagct gcaacatttc agacggcatc
     4981 attctaaaaa tgtcagacat cgggaactta atcagggttt gtggcagatt gatattgaat
     5041 taggaaaagg tctgaaaccg caagcagcat ggtaaggcga tgtcgaacag gtttaaagcc
     5101 aatccgctat attttttgga gctgcaccgt gcaaacaggt agaatggcgg cggttttgtc
     5161 cggtgtgaaa atgtttgaat attaggatga ttgatggagc tgatgactgt tttgctgcct
     5221 ttggcggcgt tggtgtcggg cgtgttgttt acatggttgc tgatgaaggg caggtttcag
     5281 ggcgagtttg ccggtttgaa cgcgcaattg gcggaaaagg cggcaagatg tgattttatt
     5341 gaacaggcac acgcagaaat tgcatcagaa ttggccgctt tggatgggaa atacgagcat
     5401 ttgcaggacg aaaattatgc tttgggcaac cgtttttccg cagccgaaaa gcagattgcc
     5461 catttgcagg aaaaagaggc ggaatcggtt cggttgaagc agtcgtatat cgatttgcag
     5521 gaaaaggcgc agggtttggc ggttgaaaac gaacgtttgg caacgcagct cgggcaggaa
     5581 cggaaggcgt ttgccgagca atatgcgctg gaacgccaaa tccgccaaag agtcgaaacc
     5641 gatttggaag aaagccgcca aactgtccgc gacgtgcaaa acgacctttc cgatgtgggc
     5701 aaccgttttg ccgccgccga aaaacagatt gcctatttgc aggaaaaaga ggcggaagcg
     5761 gagcggttga ggcagtcgca taccgagttg caggaaaagg cgcagggttt ggcggttgaa
     5821 aacgaacgtt tggcaacgca aatcgaacag gaacgccttg cttctgaaga aaagctgtcc
     5881 ttgctgggcg aggcgcgcaa aagcttgagc gatcagtttc aaaatcttgc caacacgatt
     5941 ttggaagaaa aaagccgccg ttttaccgag cagaaccgcg agcagctcca tcaggttttg
     6001 aacccgttga acgaacgcat ccacggtttc ggcgagttgg tcaggcaaac ttatgataaa
     6061 gaatcgcgcg agcggctgac tttggaaaac gaattgaaac ggcttcaagg gttgaatgcg
     6121 cagctgcaca gcgaggcaaa ggccctgacc aacgcgctga ccggcacgca gaacaaggtt
     6181 cagggcaatt ggggcgagat gattctggaa acggttttgg aaaattccgg ccttcagaaa
     6241 gggcgggaat atgtggttca ggcggcatcc gtccgaaaag aggaagacgg cggcacgcgc
     6301 cgcctccagc ccgacgtttt ggtcaacctg cccgacaaca agcagattgt cattgattcc
     6361 aaggtttcgc tgacggctta tgtgcgctac acgcaggcgg cggatgcgga tgaggcggca
     6421 cgcgaactgg cggcgtatat cgccagcata cgcgcgcata tgaaaggctt gtcgctgaag
     6481 gattacaccg atttggaagg tgtgaacaca ttggatttcg tctttatgtt tatccctgtc
     6541 gaaccggcct acctgctggc gttgcagaat gacgcgggct tgttccaaga gtgtttcgac
     6601 aaacggatta tgctggtcgg ccccagtacg ctgctggcga ctttgcggac ggtggcgaat
     6661 atttggcgca acgagcagca aaaccaaaac gcgctggcga ttgcggacga aggcggcaag
     6721 ctgtacgaca agtttgtcgg cttcgtacag acgctcgaaa gcgtcggcaa aggcatcgat
     6781 caggcgcaaa acagctttca gacggcattc aagcaacttg ccgagggacg cgggaatctg
     6841 gtcggacgcg ccgagaaact gcgtctgttg ggcgtgaagg caggcaaaca gcttcaacgg
     6901 gatttggtcg agcgtgccaa cgaacagacg gcatttgctt tgggcaaacc ttcggaacag
     6961 gaagcggctg acgaagccga ataggaaagg cggatgcgtt tcagacggca tcagtgcaga
     7021 tgatgccgtc tgaagacttt ggaaagtcgg ttagaaaaac ggattgtgcc gtttttcgtg
     7081 tccgatggaa gtcatacgcc cgtgtccggc gacaacttgc acggtttcgg ggaaggcgaa
     7141 taatttgttg cggatattat tgattaagtc ggcgtggttg ccgcgcggaa aatcggtcct
     7201 gcctatggtt tcgtaaaaca gcacgtcgcc cgcaatcagc agctccgctt cggcacaata
     7261 gaaaaccacg tgtccgggcg tgtgccccgg aatatgcagc acttggaagg catagcgtcc
     7321 gaccgtgagc gtttcgcctt cttcgagcca acggttcggc gcaaaggcgg gcgagacggg
     7381 gaatccgtat tgcgcggtgg tttgcggcag cgattggagc aggaattcat cttcgcggtg
     7441 cgggccgagg acggggactt tatgcgtttc cagcatctcg accacgccgc ccgcgtgatc
     7501 gagatgaccg tgcgtcagcc agattgccgt gagcgtgagt ttgcggtttg ccaacgcttg
     7561 cagcaggaac ggcacgtcgc cgccgacatc ggtcaggacg gcttcgccgc tttcgtcgtc
     7621 ccaaatcagg gtgcagtttt ggcggaaggg ggtaacgggg agaatttcgt aacgtagggt
     7681 catcggcgtg ttcctaaaac ggtttttcag acggcatcgg gtttgccgtt tgttttatgg
     7741 cggtttgccg cctgttttga tattggggga atcaggtgat taagaagata atcggcggca
     7801 tcataccgat ttttacggcg gttttcatcc ctgcatcggc aggcgcggcg gatttgatgc
     7861 tggcgcagga atacaaaggg caggacattg ccggctgggc gatgagcgag aagctcgacg
     7921 gcgtgcgcgc gtattgggac ggaaagcacc tgataagccg tcagggttat gcgtttacgc
     7981 cccccaaagg ctttaccgcg cagtttccgc cttatccttt ggacggcgaa ttgtatagcg
     8041 gacgtggtca gttcgagcag atttccgcta ccgtgcgttc tgtttcttca gactggcgcg
     8101 gcatccgcct gcacgttttc gatgtaccca aggcgcaggg caacctctac caacgtttgg
     8161 cagtcgcaac gcagtggctg aaaacgcatc cgaacgcgcc gattaccatc atcccgcaaa
     8221 tcaaagtgcg cgaccggcgg cacgcgatgg actttttaaa acaaatcgaa gcgcagggcg
     8281 gcgaaggcgt gatgctgcgc cagcccgaat cccgttacag cggcggcagg agcagccaat
     8341 tattgaagct gaaaagccaa tacgacgacg aatgcacggt aacgcggcac tatgagggca
     8401 aagggcgaaa cgccggacgg ctgggcgcgg tcggctgcaa aaaccgacac ggcgaattcc
     8461 gcatcggcag cggtttcaag gacaaagacc gcgacaaccc gcccaaaatc ggcacgctga
     8521 ttacctaccg ttaccgtggc tttacgcgga aaggcacgcc gaaatttgcc accttcgtgc
     8581 gcgtgcgtac cgaccgttga gcaggacggc tcggacggca gccgatactt tggtttataa
     8641 ttttcctttt accgaccgat tccgacatat gaccgattta gaaaccaaac gccttgaaac
     8701 acaggcgatg cttgaaaacg ccgaactttt gttcgaccaa gggcaatgcc gcgccgcact
     8761 gcaaaaagtg gcgtacgaga ttacgcgcga tttgggggat aaatatccgt tgctcctgcc
     8821 cgtgatgggc ggggcggtgg tgtttacggg gcagttgctg ccgctgttgc gctttccctt
     8881 agactttgat tatgttcacg tttcccgtta cggcgacaag ctggagggcg gcgcgttcaa
     8941 ctggaagcgt atgcctgatg cggaacaaat ccggggcagg cacgtggtcg tgttggacga
     9001 tattttggac gaagggcata cgatgtccgc cattcaagcc aaacttttgg aaatgggtgc
     9061 ggcaagctgc cgtgcggcgg tgttcgccaa taaattaatc gacaaagaaa aaccggtcaa
     9121 agccgattat gtcggactgg atgtgccgaa ccgttatgtt ttcggttacg gcatggatgc
     9181 ggcgggctgc tggcgtaatt tgggcgagat ttacgcattg ggcggaaaat aagggatgcg
     9241 atgccgtctg aaggcctttc agacggcatc gcgcccatac gccggcagga ttaatgagga
     9301 acaggacgca tcaatatgat agggctttta atcatcacac acgaaaccat aggcgaagcc
     9361 taccgcaagc tggcgcatca tttttttccg ggcgggctgc ctgaaaacgt ccgcatactc
     9421 ggcgtgcagc cgacggaagg ccaagacgac attatcaaca acgccatttc cgcgctgcaa
     9481 gagtttcctg aaaaccacgg tgtattgatt atgaccgaca tcttcggcgc gaccccctgc
     9541 aatgccgccc gccggctcgt gcgcgaaaac aaatcggcga ttttgaccgg gctgaacgcg
     9601 ccgatgatga ttaaggccgt ccaatattcg ccggcggcgg aagactttgc cgcctttacc
     9661 gaatgcgtca gggaggcggc ggtaaaaggc attttcgcca tcacgtccgc gcccgaagat
     9721 ttggtgtgcc ggcgcagcgg cgatgccgtc tgaagaagcg gcagggcagg aaaacatttt
     9781 aaacggcgtc tgctgccgat atatagcgga ttaacaaaaa tcaggacaag gcggcgggcc
     9841 gcagacagta cggatagtac ggaaccggtt cgcccggtgc ttcagcacct tggagaatcg
     9901 ttccctttga gccaaggcgg ggcaacgccg tactggtttt tgttaatcca ctatacataa
     9961 cacgggaacc gaaatgctca aacaatccat cgaaatcatc aacaaactcg gactccacgc
    10021 ccgcgcgtcc agcaagttca cccaaaccgc gtcccaattc aaaagcgaag tctgggttac
    10081 gaaaaacggc agccgcgtca acggcaaaag cattatgggg ctgatgatgc tcgcagccgc
    10141 caagggtacg gtcatcgaac tggaaaccga cggcttggac gaagccgcag ccatgaaggc
    10201 attgaccgac ttaatcaacg actacttcgg cgagggcgaa taatgagtat cgtgctgcac
    10261 ggcgtggcgg cgggcaaagg cattgccgtc ggttgcgccc acctgattgc gcgcggtacg
    10321 gaggaagtgc cgcagtatga tgttgctcag gcggacaccg atgccgaagc cgaacgtttc
    10381 gatgccgccg tcaaagccac gcgcaaagag ttggaacagc tccgcagcgc gattcccgaa
    10441 aacgccccga ccgagttggg cgcgttcatc tcgctgcacc tgatgctctt gaccgatgtt
    10501 accttgtcgc gcgaacccgt cgatatttta agggaacaaa aaatcaacgc cgagtgggca
    10561 ttgaagcagc agagcgacaa actcgccgcc caattcgaca atatggacga tgcctatttg
    10621 cgcgaacgca agcaggatat gctgcaagtc gtccgccgca tccacaacaa cctgatcggg
    10681 cagggcaacg agttggaggt tgccgacaac ctgtttgacg aaaccgttct gattgcaaac
    10741 gacctttcac ccgccgacac ggttttgttt aaagagcagc gcattgccgc cttcgttacc
    10801 gatgccggcg gccccaccgg gcatacggcg attttgggca ggagcttgga catcccgtcc
    10861 gtcgtcgggc tgcacaacgc gcgcaaactg attaccgaag gcgaaacggt catcgtggac
    10921 ggcatcaacg gcgtgttgat tatttcgccg gatgagtcgg tgttgaacga ataccgccgc
    10981 cgcgcccgcg aataccgcag ccacaaacgc gatttgaaca agctcaaaaa aaccgccgcc
    11041 gccaccgccg acggggtctg catcgagctt gtgggcaata tagaatccgc cgaagacgtg
    11101 aaaccgctgc acaacctcgg cgcagacggc atcgggctgt tccgcagcga gtttctttac
    11161 ctgaaccgcg atacgatgcc gtctgaagac gagcagtacg aagtgtacag cgcgattgtc
    11221 aaaaaaatga agggcaaaag cgtaacgata cggacagtcg atttgggtgt ggacaaaaat
    11281 ccgcgctggt tcgggaaaaa cagcacgccc aacggcagcc tcaaccccgc gctgggcatg
    11341 accggcatcc gcctgtgcct tgccgaaccg gtcatgttcc gcacgcagat gcgcgccatc
    11401 ctccgtgcgg cggtacacgg tcccgtccgc atgatgtggc cgatgattac ctccgtatcc
    11461 gaagtgcgcc agtgcctcat ccacctcgac accgcgcaac gccagcttgc cgaacgcggc
    11521 gatgccttcg gcgaagtcgg catcggctgc atgattgaaa ttccgtctgc cgcgctgacc
    11581 gtcggcagta ttttgaaact ggtcgatttc atctccgtcg gtaccaacga tttgatccaa
    11641 tatattctgt ccgtcgatcg cggcgacgac agcgtcagcc atctctacca acccggacat
    11701 ccttccgtgc tgaaaatgct gcaacacgtc atccgtaccg ccaaccgcat ggacaaagac
    11761 gtgtccgtat gcggcgagat ggcgggcgat accgcgttta cccgcgtttt attgggtatg
    11821 gggctgcgcc gtttttccat gaatccgaac aacatcctgc ccgtcaaaaa catcattctg
    11881 cacagcaatg ccggacagct cgaaggcgat attgcgaaag tcatccgctg cgaagacgaa
    11941 gagaagtcgg aaaagctgat caaacagatc aacagcgtgt ctgtcgcgga agaagccgaa
    12001 gtcaagggac ggaaataaat attgttgaca atgcgagcgg gttgcagtca aataacgccc
    12061 ttaaaaaata cagctgcttt ggtcaagaaa tgccgtctga aaaattttca gacggcattt
    12121 tgtttggcag gcatttctgc cgcatcatcg gaaacggacg atacgccctt catcgacgcg
    12181 gatgcggacc gtaccgtttc cggaaatacc gtccgtatgt tgtccgacag tcaggtttaa
    12241 ggtcagctcg ccgtgttcgg gatggacggc ggaaagccgg agcgagtcgg gcaggcggac
    12301 gagggacagc aggcggcatt ccgttccatg attgtccaag cacacggcat tttgcggaat
    12361 atggcggtca tcgtcggtat tgggcagccc catcagacgg gcgacctgca cgccggcagg
    12421 cgtttgaatc aaggtttcgg gcgtaccgca ttgaaggatt ttcccctcgt gcatgacggc
    12481 gatttcgtcc gccgccgtgc aggcctcttc gggcgaatgc gttaccaaaa cggcagggat
    12541 gccgcccttg cggatgcgtt cggcggtcat acggcgcagc cggtcgcgca aatgcgtgtc
    12601 caaactggaa aacgattcat ccagcaacag cagggaaggg cggacaacca aagcgcgcgc
    12661 cagtgccaac cgttgcttct cgcctccgga aagtttttca ggcttgcggt gcgcctcgtt
    12721 ttccagcccg acttcggcaa gtgccgacaa ggcgaggcgt tcggcttcgg ctttcggcat
    12781 tttttgcatt ttcaaaccga atgccgtatt ttccagcgca ctcatatggg gaaacagcgc
    12841 gtaatcttga aacatcagcg agatacggcg tttttcgggc ggcatacagg taatgttttc
    12901 cccgttcagc cgaatttcgc cgccgtccgg ccggacgatg cccgcaatca tattcagcag
    12961 ggtggatttg ccgcagcccg accgccccag tacggcgagt attttgccgc gcccgacagt
    13021 caggcagatg ttgtcggcga ccgttttgcc gccgaagcat ttgcagagtc cgttcagttc
    13081 aagcatggcg catcctataa acgtatgccg tccagccact cggacagcgg atgggcggca
    13141 tcaatcagac cgtaacggta aaacgcgtcc agcgtaacca gttgcgcgtc gtgcatcatg
    13201 tttttcgaca acatggcatc caataggccg ccaatgtcca tcttttcaaa acccgctacc
    13261 tcgccatcct gattttcagg caggaaggtt tcgggcagga cggcatcgaa cacatacagg
    13321 atttcattgt gcacacctcg gctgacgggg cgaaggctgt gcagccgcga tactgggcgg
    13381 atgagcggaa acagcgtttt atccaaaccg gcttcttcgc tgctttcgcg gcacacggct
    13441 tcagacggca tttcgccgcc ggaaacaccg ccgccggcaa tattgtcgag cttgccggga
    13501 tcgactgctt tgtgcggact gcgcctgcct atccaaaaat gccatctgcc gttcgattcg
    13561 accaaaccgt tgagatggac ggcgcggctg agtagtccga acggacggaa agcggcgcgt
    13621 tcgagcgtga acaaggggtt gccgccgccg tcggtcaggt cgaaacactc gttgcgccat
    13681 ccgtgaagca gccccgcctt gttccatgtg cgggcgaggt gctgcaagcg tccgcccata
    13741 tccggccagc cgtccgcatt cagaaaaatg ccgtctgaag actcggagca gcctgcctcc
    13801 cagtcttttt tgatgcgctc agcccattcc ggcgacagat tgcccaaagg aagacggttc
    13861 agatacagcg ttttccagca actttcggca ccgtaacttg cttttgcccg ctcgaacagg
    13921 gcatcaaggt cttgtttgct gacggattcg gtaaaacgga cggtcggcat agccggcttt
    13981 cttgaacaca atgcggaaga ttgtagccaa accgcctgaa tttgcaaccg caaacagacg
    14041 gcggcctgcc gttccgatat tgcggcagga aggaaaatcc ggcaaaaaac ggaggcggca
    14101 ggaaacagga aaaacaaaac cccctcggca ttgaaccgag gggtttgata tttggttgcg
    14161 ggagcaggat tcgaacctac gaccttcggg ttatgagccc gacgagctac catgctgctc
    14221 catcccgcgt cagaagatga aactatacgg cagatttttt atgttgtcaa acattatttc
    14281 cgtgaaaaat cataaagttt tccgtccggc ggcttatgcc tgatttgaaa tatcattttc
    14341 tttacaaaag ttcatgttcg tgatttaatt ttggttaaca tcgaaacagg ggtgctgcct
    14401 gatgttcagg cggctgagaa atacccttta cacccgatcg ggataatacc tgcgtgggga
    14461 gttttcacgg attctgtctt tcagacggca ttggttttca aatgccgtct gaaaacgcaa
    14521 aacgctcctg tttctttatt ctaaacgaga aaacaggagc attttttatg actacgccaa
    14581 aaaaaaccgc caaaatttcc ggcaacgaag cgcgcgaact ttccgacttg agcgaagaca
    14641 tcggcatccg cttcaaatat cagaactccg aacgcgtgta tctgcaaggc agccgcgacg
    14701 acatccgcgt acctttgcgc gaaatccgtc aggacgacac ctacacggcg caaggtacgg
    14761 aagccaaccc gcctattccg gtgtacgaca ccagcggcgc atacggcgat ccggcggcgc
    14821 acatcgacct gaaacaaggc ctgccgcaca tccgcacggc gtggctggac gaacgcggcg
    14881 ataccgaaat cctgcccaaa ctctccagcg aatacggcat cgaatgcgca cacgatccga
    14941 aaaccgccca tctgcgcttc aaccaaatca cccgcccgcg ccgtgccaaa gccggccgca
    15001 atgtaaccca gcttcactac gcgcgccaag gcattatcac gcccgaaatg gagttcgccg
    15061 ccatacgcga acgcatgaag ctggacgagc ttttcagacg gcctgaatac gccaagctct
    15121 tgaaacagca cacagggcaa agtttcggtg cgaacatccc gacccgtccc gaccaaatca
    15181 cccccgaatt cgtgcgccaa gaaatcgccg ccggacgcgc gattatcccc gccaacatca
    15241 accaccccga actcgaaccg atgattatcg gccgcaactt ccgcgtcaaa atcaacggca
    15301 acttgggcaa ttccgccgta acctccagcc tgaccgaaga agtcgaaaaa atggtgtggt
    15361 cgctgcgttg gggcgcggac acgattatgg acttatccac aggcgcgcac atccacgaaa
    15421 cgcgcgaatg gattatccgc aacgcgcccg tccccatcgg cacagtgccg atttaccaaa
    15481 ctttggaaaa aaccggcggc atcgccgaag atttgacttg ggatttgttc cgcgacacct
    15541 taatcgagca ggcggaacaa ggcgtggact atttcaccat acacgcgggc gtgttgctgc
    15601 attatgtgcc gatgaccgcc aaccgcctca ccggcatcgt atcgcgcggc ggttcgatta
    15661 tggcgaaatg gtgtttggca catcatcggg aaaacttcct ctacacgcat ttcgacgaaa
    15721 tctgcgaaat catgaaggcg tatgacgtat cgttcagctt gggcgacggc ctgcgccccg
    15781 gctgcattgc cgatgccaac gacgaatccc aattcgccga attgcacacc ttgggcgaat
    15841 tgaccgataa agcgtggaaa cacgacgtac aagtcatgat cgaaggcccc ggccatgtgc
    15901 cgctgcaacg cgtcaaagaa aatatgaccg aagagttgca acactgcttt gaagcaccgt
    15961 tttacacact cggcccgctc gttaccgaca tcgcccccgg ctacgaccac atcacttcgg
    16021 gcataggcgc ggccaatatc ggctggtacg gcacggcgat gctttgttac gttaccccca
    16081 aagaacattt ggggctgccc gacaaagaag acgtgcgcac cggcatcatc acctacaaac
    16141 tcgccgccca cgccgccgac ctcgccaaag gctggccggg cgcgcaatta cgcgacaacg
    16201 ccctgagcaa ggcgcgtttc gaattccgct ggcgcgacca attccgctta agcctcgacc
    16261 ccgaacgcgc cgagagcttc cacgacgaaa ccctgcccgc cgaaggcgcg aaaatcgccc
    16321 atttttgctc aatgtgcggc cccaaatttt gctcgatgaa aatcacgcag gaagtgcgcg
    16381 actacgccga caagcaaaaa gcccaacggc agggtatgga ggaaaaagcg gtcgagttcg
    16441 tcaaaaaagg cgcgaagatt tacagttgaa acgtcaagca aaaaatgccg tctgaagccc
    16501 ggaaaaaagg cttcggacgg
//
LOCUS       DAWTHD010000050        16450 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWTHD010000050 DAWTHD010000000
VERSION     DAWTHD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16450
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.050"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            153..1217
                     /locus_tag="WM704_001189"
     CDS             153..1217
                     /locus_tag="WM704_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFA9340610.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPGANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="WM704_001190"
     CDS             1358..1681
                     /locus_tag="WM704_001190"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFA9340611.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="WM704_001191"
     CDS             1755..2078
                     /locus_tag="WM704_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFA9340612.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="WM704_001192"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="WM704_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFA9340613.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="WM704_001193"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="WM704_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HFA9340614.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="WM704_001194"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="WM704_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFA9340615.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4453
                     /gene="maf"
                     /locus_tag="WM704_001195"
     CDS             3845..4453
                     /gene="maf"
                     /locus_tag="WM704_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9340616.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4462..4815
                     /locus_tag="WM704_001196"
     CDS             4462..4815
                     /locus_tag="WM704_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFA9340617.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFNAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4805..6733
                     /locus_tag="WM704_001197"
     CDS             4805..6733
                     /locus_tag="WM704_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HFA9340618.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCKRPASADADTARNVKLHARHC
                     KKQQTGREIRYQKTLQPC"
     gene            6809..7486
                     /locus_tag="WM704_001198"
     CDS             6809..7486
                     /locus_tag="WM704_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFA9340619.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7499..8905
                     /locus_tag="WM704_001199"
     CDS             7499..8905
                     /locus_tag="WM704_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFA9340620.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9023..11446
                     /locus_tag="WM704_001200"
                     /pseudo
     CDS             9023..11446
                     /locus_tag="WM704_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
     gene            11491..11736
                     /gene="rpsP"
                     /locus_tag="WM704_001201"
     CDS             11491..11736
                     /gene="rpsP"
                     /locus_tag="WM704_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFA9340621.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11752..12261
                     /gene="rimM"
                     /locus_tag="WM704_001202"
     CDS             11752..12261
                     /gene="rimM"
                     /locus_tag="WM704_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFA9340622.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12261..13010
                     /gene="trmD"
                     /locus_tag="WM704_001203"
     CDS             12261..13010
                     /gene="trmD"
                     /locus_tag="WM704_001203"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFA9340623.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13025..13390
                     /gene="rplS"
                     /locus_tag="WM704_001204"
     CDS             13025..13390
                     /gene="rplS"
                     /locus_tag="WM704_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFA9340624.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13711..14436
                     /locus_tag="WM704_001205"
     CDS             13711..14436
                     /locus_tag="WM704_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFA9340625.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14471..15346
                     /locus_tag="WM704_001206"
     CDS             14471..15346
                     /locus_tag="WM704_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFA9340626.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15423..16337
                     /locus_tag="WM704_001207"
     CDS             15423..16337
                     /locus_tag="WM704_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFA9340627.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
BASE COUNT         4268 a         5265 c         3627 g         3290 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcaaag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccggc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcatcaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagagcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaattgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg cactgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaact tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caaccgcttg tcgaacacct catcgagcta cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgcgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc ccttatccgc
     4441 ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt acttccttgc ctccttcagc
     4501 ctgattgcac tgattgccct atccctttct tgggaattat ggattgcacc gttacgcccg
     4561 ggcggttcgt ggctcgcgct caaagccctg cccctctgcc tgccgctttc aggtattcta
     4621 aagaaaaaaa tctataccta ccaatacagc tccatgctgg ttctgattta ctttgccgaa
     4681 gccgtcatgc gcctgttcaa cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca
     4741 gtattcagca tcatcttctt catatcctgc ctgtcgttcg tcaaacaata caaggaaaca
     4801 aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta
     4861 catcctgccc tgcttccttt ggataggcat catccccttt accttcgcac tcaggctgaa
     4921 accgtcgccc gacttttacc acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt
     4981 cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc gccatcagct tcctcctgtt
     5041 tgcaatggcg gcattttggt ggcttcaggc acgcctgatg aacctgattt atcccggaat
     5101 gaacgacatc gcctcttggg ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag
     5161 tttggtcgca cactacggac aagaacgcat cgttaccctg tttgcctggt cgctgcttat
     5221 cggctccctg cttcaatcct gcatcgtcgt catccagttt gccggctggg aaaacacccc
     5281 cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta atcggacaca tcgggcagcg
     5341 caacaacctc ggacactacc tcatgtgggg catactcgcc tccgcctacc tcaacggaca
     5401 acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg
     5461 tttggtcaat tcgcgcacca tcttgaccta catagccgcc atcgccctca tccttccctt
     5521 ctggtatttc cgttcggaca aatccaacag acggacgatg ctcggcatag ccgcagccgt
     5581 attccttacc gcgctgttcc aattttccat gaacgccatt ctggaaacct ttacaggcat
     5641 ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt ttcacagact tgccgcgcca
     5701 aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg
     5761 gaacagtttt gcccaacaaa ccttcctgat caatgccgaa cagcacacca tacacgacaa
     5821 cttcctcagc accttgttca cccattccca caacatcatc ctccaactcc ttgcagaaat
     5881 ggggatcagc ggcacgcttc tggttgccgc aaccctgctg acgggcattg ccgggctgct
     5941 gaaacgctcc ctgacccccg catcactttt cctgctgtgc gcgcttgccg tcagtatgtg
     6001 ccacagtatg ctcgaatatc ctttgtggta tgtctatttc ctcatcccct tcggactgat
     6061 gctctttctg tcccccgcag aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct
     6121 cggcatactg accgcctccg ccgccatatt cgcaggattg ctgcacttgg actggacata
     6181 cacccggctg gttaactcct tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg
     6241 gaaaatcaac gaactgcgct atatttccgc aaacagcccg atgctgtcct tttatgccga
     6301 cttctccctc gtaaacttcg ccctgccgga ataccccgaa acccagactt gggcggaaga
     6361 agcaaccctc aaagcactaa aataccgccc ctactccgcc acctaccgca tcgccctcta
     6421 cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta
     6481 ttacccctac ctgatgcccc gatacgccga cgaaatccgc aaactgcccg tatgggcacc
     6541 gctgctgccc gaactgctca aagactgcaa agccttcgcc gccgctcccg gccatccgga
     6601 aacaaaaccc tgcaaacgac ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct
     6661 ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa atacgctatc aaaaaacatt
     6721 gcagccgtgt taagataaac tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg
     6781 gggcataccc ttacgaaaag gaaacaccat gagccgcgta ttactcgtag atgacgatgc
     6841 cctgctgacc gaactgctga ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt
     6901 tcccgacggg gaagcaggcg tacaggaaat cctgagcggg caatacgatg tagtcgtatt
     6961 ggattccatg atgcccaaaa tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag
     7021 caccgtcccc atcatcatgc tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt
     7081 ggaaatgggt gcggacgact atgtccccaa accctgcaca ccacgcgaac tcttggcacg
     7141 catcaatgcc atcctccgcc gagcacaaca cagcggcgaa cagaacaacg cacccaacag
     7201 catctccgtc agcgatgtcg tcctataccc cgccaaacgc caggcatccg tcaaagacat
     7261 gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg
     7321 acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt
     7381 cgaccgcagt atcgacgtac acatctccag catccgccac aagctgggcg atgcctccct
     7441 gattcaaacc gtacgcggct tgggctacct gtttgtcaaa aactgaagca gcgggcaaat
     7501 gaaactgttc caacgcattt tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt
     7561 ggcgagtttt tctttttggc tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg
     7621 ccgcaccatc gaaaccacat tgatgggcag cattatttcc gcattcaaga cacggggcga
     7681 caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta
     7741 cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc tatatcgaca attacaccat
     7801 agaacgcgcc cggttgtttg ccgccaacaa cccccattcc aaccttgtcc gcatcgaata
     7861 cgaccgtttc ggcgaagaat acctgttctt cattaaaggc tgggacaacc accaggcaca
     7921 acgcctgccc agcccgctgt ttatcccggg cctgccgctt gccccgattt ggcacgaatt
     7981 catcatcctc tccttcatca tcattgtcgg actgctgatg gcatatatcc ttgccggcaa
     8041 cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa acggagaact
     8101 tgaaacccgc atctcccaac aggtcgacga ccgcgacgac gaattgtccc atcttgccat
     8161 ccaattcgac aaaatggcag aaaaactcga aaaacttgtc gccaaagaac gttacctact
     8221 ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt atgcaggcaa ttgtcggact
     8281 gattcaggca cagccccaaa aacgggagca atatctcaaa cggctggaag gcgaactgac
     8341 ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat
     8401 ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg tagaagacaa
     8461 ccaaagtatc gcccaaaaga acggacagac ggtcgccctg tctgccgacg gaaaaatccc
     8521 cgaaaacaca accatccttg ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat
     8581 ccgcaacgcc gtcaactaca gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga
     8641 ccacaaacac tggataatcg acgttaccga caacggcccc ggcgtggacg aaatgcagct
     8701 cccgcacatc ttcaccgctt tctaccgtgc agactccagc gccaacaaac ccggaacagg
     8761 actggggctt gcattgaccc aacatattat tgaacagcac tgcggcaaaa tcatcgccga
     8821 aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt
     8881 caaaacagaa aaaagtgcga actgaccata atgccgtctg aacccgcttc agacggcatt
     8941 gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaaaaa tcacgcacat
     9001 accgtccgca aaggaatata tcatgccggc acaaaccgat ccgggctact tcttcatgcc
     9061 caaccacatt atcctgatag gcgcgagcga acaaccgtac agcctgggtg aacgcgtact
     9121 cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca
     9181 caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca gtgcagacct
     9241 gattattacc gttaccccgc ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa
     9301 gcagctccga cacatcatcc tcatacagga ctgggacagc ctgtctgccg cagaactgca
     9361 caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca ccgcctacac
     9421 caccgcaggc atccaactgc cctcactcgg actcaacatc agtacccaag acggatatgc
     9481 cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat
     9541 cctgaacaaa ctccgtcaag gcacatcccg ccacatcaac ctgcatcccg gcatcagccc
     9601 catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca aaaccgccgt
     9661 cctacaccac aaccctgaag aggatcagcg caggctgttc agcgcaatcc gccaatttac
     9721 ccgccatacg ccgctgattc tccacatcac ctgcctcacg acagaaaccg accgtgccgt
     9781 actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg acgacctcga
     9841 agccgcactg agcgcccaac tgtccgacct tccacccctg tcccgactcg acatcctgtc
     9901 cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc
     9961 caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac cctcaatctg
    10021 ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc tgaccatcct
    10081 cggtccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc gcctgtccga
    10141 acagaccgcc aaacccctgc tcgtcaacag ccccttttca gacggcataa cacatttcga
    10201 cacccccgca caggcaatcc gcaccctttc ctaccgtaac accgccgccg ccctgaaaca
    10261 ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa
    10321 catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc gccgaagccc tgcacctccc
    10381 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac cccctctatg
    10441 gcgacatcct gaccgcacgc tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    10501 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgatc
    10561 agttcctgca cacactgacc gtcatccccg aataccgcca acacattttc ggcatcaccc
    10621 tcaacctcaa cggcggacaa tacagcagcg atttcatctt aaagacaccc ggaacacacg
    10681 acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa
    10741 aaatgcagag tgccgccgca tacctggaac acaaaaaccc gacagccgcc gaatttctcc
    10801 gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg
    10861 tacccaacgt acttgccccc tatcccgcag cacaccccaa aacactgtcc ctaaaaaaca
    10921 acacgaccgt taccattatc ccccttttac ccgaagacgc agaagccaaa cagcagttcg
    10981 tccgcagcct cggccccgaa gcacggtaca cacgcttcat gacccacacc aacgaactgc
    11041 ccgcagccac gttggcacgc ctgtgcaacc ccgattacca ctgtgaaccg catggacggc
    11101 aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga accgcaacga
    11161 atgcgaattt ggcatcacac tggcggaaca tatgcgcggc agcgggctgg cacagaaaat
    11221 gatggaactc atcatccaaa ccgccgcaca gcaaggctac ctgagcatga gtgccgacat
    11281 tctcaaaacc aataccccca tgatcaaact tgctgaaaaa tcaggattta ccctcaagga
    11341 atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag acaaaacaac
    11401 aggaaaaaca aataaaaact tgcgcaccgg ccacaaaata acctaaaatc ggcagtttcc
    11461 atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc acgcggcggc
    11521 tcgaaacacc gccccttcta caacgtcatc gttactgact cacgcagccg ccgcgacggc
    11581 cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca agagcgcgtc
    11641 cgcctcaatg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt cagcgactcc
    11701 gttgcaaaac tgattaaaga acaaaaagcc gtctaatccg cgtttgccgc catgacagac
    11761 actcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa aggctggctg
    11821 aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga gtggcatttg
    11881 gccaaggacg gcaaaaccgt cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa
    11941 ctccaagtca aattcgaagg catagacgac cgcgattcag cattctcatt gcgcggttac
    12001 accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata ctactgggca
    12061 gacttggtcg gcatgaccgt cgtcaacaaa gacgataccg ttttaggcaa ggtaagcaac
    12121 ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg gcagattctg
    12181 attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagac cattactgcc
    12241 gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc gaaatgttcg
    12301 acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc tggcagtttg
    12361 aagcagtcaa tccccgaaag tttgccgaca acagattggg ctatatcgac gaccgcccgt
    12421 tcggcggcgg tccgggaatg attatgatgg ctccgccgct tcatgcggcg atagaacacg
    12481 ccaaagcaca atcttcccaa accgcaaaag tcatctacct cagcccccaa ggaaaaccgc
    12541 tgacacacca aaaagcggca gaactggcag aacttacgca tctgattctg ctgtgcggac
    12601 gctatgaggg aatagacgaa agactgctgc aaagcagcgt cgatgaagaa atcagcatcg
    12661 gagacttcgt cgtttccggc ggagagcttc ccgccatgat gctgatggat gcggtattga
    12721 ggctcgtacc cggcatattg ggcgacattc agtctgccga acaggattcg ttctcaagcg
    12781 gtattttgga ctgcccccac tacaccaaac ccttagaatt tcaaggcatg gctgttccgg
    12841 aagtattgcg ctccggaaat catggcttga tagcggaatg gcggttggaa caatcgctgc
    12901 gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg cgttttaatc ccaaaggaat
    12961 cccgcctctt gaataaaatc ctacaagagc aacgggaaat ccaatcataa tttaggaaaa
    13021 aacaatgaac ctgattcaac agctcgagca agaagaaatt gcccgcctga acaaagaaat
    13081 ccccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtgg aaggtacccg
    13141 cagccgtctg caagcctacg aaggcgtggt tatcgcccgt cgcaaccgtg gtttgaacag
    13201 caacttcatc gtccgcaaaa tctccagcgg cgaaggtgtt gaacgtactt tccaactata
    13261 ttcccctact gttgagaaaa tcgaagtcaa acgccgtggc gacgtacgcc gtgccaaact
    13321 gtactacctg cgcggtctga ccggcaaagc tgcacgcatc aaagaaaaac tgcctgcacg
    13381 caaaggttga ttcaaaccgt tttcccccaa tgccgtctga acgttcagac ggcatttttt
    13441 attgccgcga tgccggcaag gcaacgatat gcctgaccct catccacctt aattaacaat
    13501 cactcccgcc tattcaagca gaatttacag actaaacgtt atacagtatc atatccgcca
    13561 tataacgata accgctttgc caacttccct ttcaaaactg caaatcatta tgacaccttc
    13621 cctcttactg tcaggattga ccttccgact cttcctcgcc ctgattgccg tattcctttt
    13681 atggggtgtt tacctttggg cggtatcggt atgagcatca tcgttgaaaa cctgacggtc
    13741 agctaccgcc gccgccccgc cgtacaccac gtcgacatta cttttgaaga acatagtatg
    13801 tgggcggttt tcggtcccaa cggcgcagga aaatctacct ttctcaaatc cttaatggga
    13861 ttgcagccta tcgatacggg cagcatccgg ctggacggat tgaccagtca aaacattgcc
    13921 tatctccccc aacaatccga catcgaccgt tcccagccta tgaccgtttt cgacttggcg
    13981 gcaatggggc tgtggtatga aatcggtttt ttcaaaggga taaataccgc tcaaaaacaa
    14041 cgcgttcacg aagccttgga gcgtgtcggt atgcggcagt ttgcccgccg ccaaatcgcc
    14101 cacctctcaa acggacaatt ccaacgcgtc cttttcgccc ggatgctggt tcaaaatgca
    14161 aaattcctgc ttttggacga acctttcaat gctgttgatg cacggacaac ctacgagctt
    14221 ctcgacgtgt tgcaaaaatg ccattgcggc ggacacgcta tcattgccgt tttgcacgat
    14281 tacgaacaag tccgtgccta ctttcccaat accctgctgc tcgcccgcga aaaaattgcg
    14341 gcaggcgcaa ccgagaccat tctgacagaa tcctacctcg cccaagccag cgccaaaatg
    14401 cagcaacagg aaagccccga ctggtgcgcc tcataaatac tgcccgaaac cacgccccac
    14461 accagacacc atgaacctct acgacctgct ccttgccccc tttgcagaat tcgacttcat
    14521 gcactacgcc ctcgcatccg tcttctgcct gtccctcagc gccgcacccg tcggcgtatt
    14581 cctcgtcatg cgccgtatga gcctgatagg cgacgcattg agccacgccg tcctgcccgg
    14641 tgccgccgtc ggctacatgt ttgccggctt gagcctgccc gctatgggtg tgggcgggtt
    14701 tgccgccggt atgctgatgg cgctgcttgc cggactcgtc agccgcttta ccaccctgaa
    14761 agaagatgcc aactttgccg ccttttacct gagcagcctc gccatcggcg taatcctcat
    14821 cagcaaaaac ggcagcagcg tcgatttact ccacctcctt ttcggatctg tgcttgccgt
    14881 cgatattccc gcactgcaac tcatcgccgc cgtctccggc ctcacgctca ttacccttgc
    14941 cgtcatctac cgccccctgg tgctagaaag catagacccc ctgttcctca agtccgtcaa
    15001 cggcaaaggc gggctttggc acgtcgtttt cctcatcctc gtcgttatga acctcgtatc
    15061 cggcttccaa gctctcggca ccctgatgtc ggtcggactt atgatgctgc ccgccattac
    15121 cgcccgctta tgggcaagaa atatggggac gctcattctg ttgtccgtcc tcatcgccct
    15181 tttttgcggt ttgatcgggc tgctcatttc ctaccacatc gaaatccctt ccggccccgc
    15241 catcatcctc tgttgcagcg tcctttatct cttttccgtc atactcggca aagaaggcgg
    15301 catcttgccc aaatggttca aaaaccaccg ccaccacacc acctgacccc gcaagccgct
    15361 tgaacaaaca ggcggcttgt tttaccataa tgaatgttat gttatatcac agaagaacag
    15421 ttatgaaaca cctcaaactc acccttatta ccgcattgct ggccaccgcc gcaactgccg
    15481 caccccttcc ggttgtaacc agtttcagca ttttaggcga cgtagccaaa caaatcggcg
    15541 gtgagcgcgt agccgtacaa agcctcgtcg gagccaacca agatactcat gcctatcaca
    15601 tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact cgtcctgctc aacggcttgg
    15661 gacttgaagc cgccgacatc caacgcgccg tcaaacagag caaagtatcc tatgccgaag
    15721 cgaccaaagg catccaaccc ctcaaagccg aagaagaagg cggacaccat cacgaccacg
    15781 atcatgacca cgaaggacac caccacgacc acggcgaata tgacccccac gtctggaacg
    15841 accctgttct tatgtccgac tatgcccaaa acgtcgctga agccctgata aaggccgatc
    15901 ccgaaggcaa agtttattat caacaacgct tgggcaacta ccaaatgcag cttaaaaaac
    15961 tgcacagcga cgcacaagcc gcatttaatg ccgtccctgc cgccaaacgc aaagtcctga
    16021 ccgggcacga cgcattttcc tacatgggca accgctacaa catcagcttc atcgccccgc
    16081 aaggcgtgag cagcgaagcc gagccgtccg ccaaacaagt cgccgccatc atccggcaaa
    16141 tcaaacgcga aggcatcaaa gccgtattta ccgaaaatat caaagacacc cgcatggttg
    16201 accgcatcgc caaagaaacc ggcgtcaacg tcagcggcaa actgtattcc gacgcactcg
    16261 gcaacgcgcc cgcagacacc tacatcggca tgtaccgcca caacgtcaaa gccttgacca
    16321 acgcgatgaa gcaataaaac cgtttgattg gtttgaattt gaaaaggcaa aaggccgtct
    16381 gaaaattaaa tacgaggttt tcagacggcc ttctcttgtg caataaagca gtaaacaaat
    16441 attgataaga
//
LOCUS       DAWTHD010000051        16255 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWTHD010000051 DAWTHD010000000
VERSION     DAWTHD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16255
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.051"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(277..1062)
                     /locus_tag="WM704_001208"
     CDS             complement(277..1062)
                     /locus_tag="WM704_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFA9340628.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1306..2121
                     /locus_tag="WM704_001209"
     CDS             1306..2121
                     /locus_tag="WM704_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFA9340629.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2162..3031
                     /locus_tag="WM704_001210"
     CDS             2162..3031
                     /locus_tag="WM704_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFA9340630.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3067..4323
                     /locus_tag="WM704_001211"
     CDS             3067..4323
                     /locus_tag="WM704_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFA9340631.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(4381..4521)
                     /locus_tag="WM704_001212"
     CDS             complement(4381..4521)
                     /locus_tag="WM704_001212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340632.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            4669..5097
                     /locus_tag="WM704_001213"
     CDS             4669..5097
                     /locus_tag="WM704_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9340633.1"
                     /translation="MNFALSVITFTLASFLPVPPAGTAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5146..6489)
                     /locus_tag="WM704_001214"
     CDS             complement(5146..6489)
                     /locus_tag="WM704_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFA9340634.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6494..7147)
                     /locus_tag="WM704_001215"
     CDS             complement(6494..7147)
                     /locus_tag="WM704_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFA9340635.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7297..7587
                     /gene="gatC"
                     /locus_tag="WM704_001216"
     CDS             7297..7587
                     /gene="gatC"
                     /locus_tag="WM704_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFA9340636.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            7650..9095
                     /gene="gatA"
                     /locus_tag="WM704_001217"
     CDS             7650..9095
                     /gene="gatA"
                     /locus_tag="WM704_001217"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFA9340637.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9092..10024
                     /locus_tag="WM704_001218"
     CDS             9092..10024
                     /locus_tag="WM704_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFA9340638.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVTKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            10067..11497
                     /gene="gatB"
                     /locus_tag="WM704_001219"
     CDS             10067..11497
                     /gene="gatB"
                     /locus_tag="WM704_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFA9340639.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11589..12599
                     /locus_tag="WM704_001220"
     CDS             11589..12599
                     /locus_tag="WM704_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HFA9340640.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(12729..13361)
                     /gene="pdxH"
                     /locus_tag="WM704_001221"
     CDS             complement(12729..13361)
                     /gene="pdxH"
                     /locus_tag="WM704_001221"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFA9340641.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13421..13573
                     /locus_tag="WM704_001222"
     CDS             13421..13573
                     /locus_tag="WM704_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340642.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13582..14631
                     /locus_tag="WM704_001223"
     CDS             13582..14631
                     /locus_tag="WM704_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HFA9340643.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14690..16231)
                     /locus_tag="WM704_001224"
     CDS             complement(14690..16231)
                     /locus_tag="WM704_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFA9340644.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYVGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIVGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         4131 a         4955 c         4016 g         3153 t
ORIGIN      
        1 gtacagaccc ttgtttttta ttccccggtg tctgttttct gccacagtcg atttagattc
       61 cagcaaaccg cctttaaaca attgaaagaa aagggacaaa ttgcacaggg ttctccgttt
      121 aaccagatac ggatttccat tcccggcaat ttcgggcatt tgaccgtttc cgccttcctg
      181 ccccgccgcc aataaaaatg ccgtctgaaa tatcggcacg atacttcaga cggcataccc
      241 gcccgttttc ccgcatttcc gggagctggc tgaaatttaa acgtgtgcgg aaatgatttt
      301 taacatttgc gccagtactt tgggattggc agccacaata tcgccgcttt ccaaccaagc
      361 gtcctcaccc gacatatcgg taacgatgcc gcctgcttct tggacaatca atgcaccggc
      421 ggcaatgtcc cacggtttga ggttgaactc gaaaaagccg tcaaaacgtc ctgttgctac
      481 ggcgcacaaa tccaaagaag ccgcgccttc gcggcggcct ccggcggttt ttgccaaaaa
      541 gtctttcaaa atcgccagat atttgtccat catgctttga tcgacaacag ggaaaccggt
      601 accgatcagg cagcggttca attcgatgcg gttggagaca cggatgcggc ggtcgttaag
      661 caacgcgcct ttaccgcgcg aagccatgta cacgtcgtta cgttcggggg cgtacaccaa
      721 agcttcctgc aacacgcctt tgtgcagcag tgccatagag atggcgtatt ggggatggcc
      781 gtgaaggaaa ttggtcgtcc cgtcgagcgg atcgataatc cattcgtact cggctgtagc
      841 tttgccgtgg aagccgcttt cttcgcaagt gattttgtga tgcggatagg cttctttcaa
      901 agcctcaacc aggatgattt cggaattgcg gtcaacatcg gaaacaaaat cgttgaaggc
      961 tttgctgtcg gttttgacgg catcgaggtt acctgcggcg cgaatcatca tctgaccggc
     1021 acggcgggcg gctttaaagg ctgtattcaa aaacggattc atcagatttc cttaagggtg
     1081 gcataccgcc ggttcggacg tgcagtcctt cggagcggca aaatcggagt ttatctggtt
     1141 ggggcaaacc ctgccaaatc gggtaaaata ccgcctgacg cgtgtctgct tcaggcgcaa
     1201 cgttaaattt ccgacgttgt taaagaacag ttcaaacggc atttcaccgt ccgaacgaaa
     1261 aagacggcgc attataccct attccattcc gaccgaaaac cgaacatgac tgctctcaaa
     1321 cccgccctgc ccgactacct cggcaacatc cgcatcatcc tcacacgcac cagccacccc
     1381 gccaacatcg gctctgccgc acgcgcgatg aaaacgatgg gtctgcacag gctgaccatc
     1441 gtcaccccca atctgatggc aacgccgatg acggaaaatc cgcccgtttt taatcccgat
     1501 gacgtgcaaa gttttgcact gcctgaagaa agtttcatcc tcgcttccgg cgcggcggac
     1561 gtattgcaca acgccgaaat cgtcgccaca ctggacgaag cccttgccga caccaccatc
     1621 gcctgcgccc tgaccagccg ccgccgcgaa atcaccgcgc cgctgcaaac cccgcgcgat
     1681 ttggtgcccg aattactgca ggccgccaac cgcggcgaga aagtggcgct ggtcttcggc
     1741 aacgagactt tcggcttgag catcgaagaa gtccgagcgt gcaaccgact gatgaccatc
     1801 aacggcaacc ccgactattt ctcgctcaac ctcgcccaag ccgtgcaggt cgtgtgctac
     1861 gaaattttca gccaaaccga ttcgcccatg acccatcttc aacaggaaga ccacgccgca
     1921 acccacgagc aaatcaaagg catgctcgcc cacatggaaa gcgtgatgga cgacatcggc
     1981 tttttcaacc gccgcaacgg cgagcgtctg atgcgccgta tgcagagcct gttcggacgc
     2041 gccaacacgc aaaccgaaga catcgacatc ctgcgcggtt ttttcaatac cgtcagccac
     2101 cgtatccata aaaaagactg attaaggccg tctgaaaaca ttacaagctt tttcagacgg
     2161 catgactgat attcggataa gcatgaatta cgccctagac gcattatggt ggaaactcac
     2221 cagccaaccc gtccgcgacc tcgcctcgct gctgactgcg ccgcctttgt ggcaaagcgg
     2281 ttgcgaattg agcgtgcgcg aactactggg ggaacgcggt ttccgctacc ttttagcatt
     2341 ggatgccgat cccgcgccgc tgacggatta ccttgcacaa cgcgccccgt tcgaccaccg
     2401 tctcggcatt tatgccgaag agctgctggc tttttggttt accaacgcac cgcacaccaa
     2461 actgcacgca tataatctga ccgttttttc agacggccaa actttaggcg ccgcggattt
     2521 tgtcgtttcc ctcaaccaac agccctatca tatcgagctg gcgtgtaaat actacggcgg
     2581 agaccaagtg cagaacctgc gcggtctcaa tcccaaagac acgctgacgg acaaagccgc
     2641 caaattggtg cagcaattcc agctggtgca tacgtcgcaa ggcaaagcaa ccttagccgc
     2701 acaagacctg ccggaaaatc cacttcctgc ttccatcgtg cgcggcatcg gattttttcc
     2761 acaaggtttc catgcttttg agccgccgct taatccatac ggctggcgcg gcatctatat
     2821 tcaagattgg gcggaatacg ggtttgaacg ccaagaagcg cgctatcacc tgctcgaccg
     2881 catggtctat ctcgcgcctg cgcgtgtcgc cgaaaccgaa acattaaacg caaccgaaat
     2941 ccgccgcatc gaccaaggct tgattgccgt tttggaatgt cggccggacg gcttttggca
     3001 cgaaatcgaa cgtattatga aggccgtctg aaacccttta ccaactttaa gagaacccat
     3061 tacattatga acgccgcaca actcgaccat accgccaaag ttttggctga aatgctgact
     3121 ttcaaacagc ctgccgatgc cgtcctctcc gcctatttcc gcaaacacaa aaagctcggc
     3181 cgccaagacc gccacgaaat cgccgaaacc gcctttgccg cgttgcgaca ctatcaaaaa
     3241 atcagtaccg ccctgcgccg tccgcacgcg cagccgcgca aagccgcact cgccgcgctg
     3301 gttctcggca gaagtaccaa catcagccaa atcaaagacc tgcttgatga agaagaaaca
     3361 gagtttttcg gcaatttgaa agcccgtaaa accgagtttt cagacggcct gaataccgcc
     3421 gcagaattgc cgcaatggct ggtggaacaa ctgcagcagc attggagcga agaagaaatc
     3481 ctcgctttcg gccgcagcat caaccaagct gcgccgctcg acatccgcgt caataccttg
     3541 aaaggcaaac gcgacaaagt attgccattg ttgcaagccg aaagccccga tgcagaggca
     3601 acgccttatt ctccttgggg catccgcctg aaaaacaaaa tcgcgcttaa caaacacgaa
     3661 ctgtttttgg acggcacgct ggaagtccaa ggcgaaggca gccagctgct tgccttattg
     3721 gtgggcgcaa aacgcggcga aatcattgtg gatttctgtg ccggagccgg cggtaaaacc
     3781 ttggctgtcg gcgcccaaat ggccaacaaa ggcagaatct acgccttcga catcgccgaa
     3841 aaacgccttg ccaacctcaa accgcgcatg acccgtgccg gactgaccaa catccacccc
     3901 gaacgtatcg gcagcgaaca cgatacccgt atcgcacgac tggcaggcaa agccgaccgc
     3961 gtgttggtcg atgcgccctg ctccggctta ggcacactgc gccgcaatcc cgacctcaaa
     4021 taccgccaat ccgccgagac cgtcgccaaa cttttggaac agcaacacag tatcctcgat
     4081 gccgcctcca aactggtgaa accgcaaggc tgtttggttt acgccacctg cagcgtgttg
     4141 ccggaagaaa acgaactgca aatcaaacgt ttcctgtccg aacatcccga atttgaaccc
     4201 gtcaactgcg ccgaactgct gcaaaacctg aaggtcgatt tggataccgg caaatacctg
     4261 cgcctcgatt ccgcccgaca ccaaaccgac ggcttcttcg ccgccgtatt gcaacgcaaa
     4321 taaaccggtt tgaacaaaat gccgtcagaa cccttttcaa agcgttcgga cggcatttca
     4381 tcaattaaaa atatcaaaaa ccgcagattc aaccgattca acaccaaagg cagaatcacg
     4441 cgcctcatcg gattttatac gcgcctgcct gcaattttct ttttctttgc caaaatatac
     4501 ggtagcataa ccatattcca atgatatttt tgggaatctg ttttacccca atatataaag
     4561 caccatatta aggcggagtg tcttccctgc cttgacccga acccggaaaa gacaccgccc
     4621 gaaccaatcc cgattctgcc cgggcagtca gccatcaagg aaaccctaat gaactttgct
     4681 ttatccgtca tcacatttac cctcgcctct ttcctgcccg tcccgcctgc cggaaccgcc
     4741 gtctttactt ggaaagacgg cggcggcaac agctattcgg atgtgccgaa acagcttcat
     4801 cccgaccaga gccaaatcct caacctgcgg acgctccaaa ccaaaccggc ggtcaagccc
     4861 aaacctgccg tcgatacgaa tgcggacagt gcgaaggaaa acgaaaagga tatcgccgag
     4921 aaaaacgggc agcttgagga agaaaagaaa aaaattgccg aaaccgaacg gcagaacaaa
     4981 gaagaaaact gccggatttc aaaaatgaac ctgaaggcgg tgggaaactc aaatgcgaaa
     5041 aacaaggatg atttgatccg taaatacaat aacgccgtaa acaaatactg ccgttaatcg
     5101 gctctagcgc aaacccgatg ccgcctgaag cggcacgggg tttgtttatt tctgccagta
     5161 ggttttgacg ttgacgaact cgtacaaccc gaattcggac agttcgcgcc cgtaaccgga
     5221 atctttgact ccgccgaaag gcaggcgcaa atcgctgctg gtatggcggt tgataaacac
     5281 cgatccggcc tgtatttttt cggcaaaccg ccaggcgcgt tcagtatcgg cggtataaat
     5341 gcaggcaccg agcccgaacg gggaatcatt ggcaaggcag atggcgtgtt cttcgttttc
     5401 ggcgcgcaaa atcagggcga cggggccgaa tacttcttct ctccagacgc ggcaggcagg
     5461 atttaccctg tctaaaaccg tcgcagggta aaaccacccc gacccctgtg gaactttgcc
     5521 tccggtcagg cacaccgcgc cgtttgaaac ggcatcttca acctgcccgt gaacccggtc
     5581 ccgcaaatct tcgcggtgca gcggtgcaag cgtggtatcg ggatgtttcg ggtcgcccat
     5641 tttcaatttg gcgcattcgg cgagaaacag ctgaataaag cggtcggctg cggcttcggt
     5701 tacgatgatg cgcttggcgg cgttacacga ttgccccgca tcgcggaaac gggaataaca
     5761 ggcttctgcg gcggcgcgtt ccaaatcggc atcgggcatc acgataaagg cgttgctgcc
     5821 gccgagctcc aacacggttt tcttgaggtt tgcgcccgca tgtgccgcaa gaatgcgccc
     5881 cgtatgcgtc gaaccggtaa acgccatcgc atcggtatct tcgactgcct tgagcgtgtc
     5941 cgcctcatcc agccacgcgc ctgccagagg aataccgtct gaagccaaat cgaacaacgc
     6001 ccgactgacg cgtgccacgc tgggcgcggg tttgacggcg cacgcgttgc ccgcgcacat
     6061 agcgggaacg gcaaaacgca atacctgcca gacgggatag ttccaaggca tgacggcaaa
     6121 caccacgccc aaaggctcga agcgcacctg actcaaactc gcctgcgtcg caatggtttt
     6181 gtgggcgagc agttcggggg cgaggcgggc gtaatagcgt atcagttcga tagacttgac
     6241 gatttcggca cggcattcgt gcaggcagcg tccgacttct tcgcacacca tttccgcaaa
     6301 acgctctttt tccgcctcca aacggccggc aaatttttgc agacgcgcgg cacgttcggt
     6361 tacgcccaat tgcgcgaacg ccctgccgtg cattttcaaa tccgccagcc gccgttcaaa
     6421 ctccgcataa tcttgatcgg ggcggcggta aagcgtttcg cccgtaaata cattgacact
     6481 gtgaaacatc gaatcaacct gccagttgcg ggaatatcgt tttcagtccc gacacaataa
     6541 tctccaccga taccgccgcc agcatcatac ccataatgcg gtttaaaatc gtcagccccg
     6601 tcgcgcccag caggcggctg accttcccgg caacgattaa aatggcataa caaatcgcac
     6661 tgaccaccaa accggccgcg ataatcagcg cgatatcgct gtacgttttg gctgccgaag
     6721 cgtaaataat cacggtcgaa ataccgcccg gaccgatggt gatcggtatg gcgatgggca
     6781 cgacggcaat cgcccctgca ttgcgggcgg ggcgcgcttg ccccgtttcc ggctgcgcgc
     6841 cgagattctg cttggcggga ttgtcgttgc cgttcatcat cgaaatggcg atcagcagca
     6901 ccaaaatccc gccgccgacc tgaaacgaac cgacgctgat gcccaaaacc ttcaatagcg
     6961 caccgccgat cagcgcaaat accgcaatca cggcaaacac ggcgacggcg gccgtccgcg
     7021 cgaccttcct gcgctccttc gtgctgtgtc cgttggtcag gtcaaggtaa agcgacaacg
     7081 cgctaaacgg attgatcagc accaaaaaag ccacaatcag cttgccgatt tccataccca
     7141 atcccattat ttcccctctt tcaaacccgt gcggcaggca tccgatgctg caaattgccg
     7201 ccgcaacgga tttttccgtt ataattaaaa atccaagcaa tacgccccat catacccgaa
     7261 cgacggtatc tttaccatca gacaaggatg cttttcatgg cactgacact tgccgacgta
     7321 gacaaaatcg cccgactctc ccgactgcag ctgactgcgg aagaaaaaga aaaatcgctt
     7381 caagaattaa acgacatttt cactatggtc gaacagatgc agaacatcaa cacagacggc
     7441 atcgagccga tggcgcaccc gcacgaggtc gccctccgcc tgcgtgaaga cgaagtaacc
     7501 gaaaccgacc gcgccgccga ataccaagcc gttgcgccgg aagtacgcaa ccgcctgtac
     7561 atcgtaccgc aagttatcga agaataatcc gaatacgctt cagacggcat cagcaatacc
     7621 gcccgaagcc ctttaaggat ggaagattta tgacccaata cacattgaaa caggcaggca
     7681 gcctgttgca gtccaaacag atttccgccg tcgaactggc aagcgcatac cttgccgcca
     7741 tcgctgaaaa aaatcccgcc ctcaacggct atatcaccat cgaccaagat aaaacccttg
     7801 cagaagcccg tgccgccgac gaacgcatcg cgcagggcaa cgcttcggca cttaccggcg
     7861 ttcccgtcgc ctacaaagac attttctgcc aaaccggctg gcgcagcgcg tgcgcttcca
     7921 aaatgctcga caacttcatc tccccctaca ccgccaccgt cgtccaaaac ctgctcgacg
     7981 aaggtatggt aacgctcggc cgcaccaaca tggacgagtt cgctatgggt tcgaccaatg
     8041 aaaactcatt ctacggcgcg gccaaaaacc cgtggaatcc ggaacacgta cccggcggtt
     8101 cgtcaggcgg ttccgccgcc gtcgttgccg cgcgcctcgc ccctgccgcg ctcggttcgg
     8161 acacgggcgg ctctatccgc caacccgcat cgcactgcgg cattaccggc atcaaaccta
     8221 cctacggcac ggtttcccgc ttcggtatgg tcgcctacgc ctccagcttc gatcaagccg
     8281 gcccgatggc gcaaaccgcc gaagactgcg cgattctgtt gaatgcaatg gcaggtttcg
     8341 accccaaaga ctccaccagc ttcgaacgcg aaaaagaaga ctacacccgc gatttggaca
     8401 aaccgctcaa aggcgtgaaa atcggcctgc ccaaagaata cttcagcgaa ggcaacagca
     8461 ccgatgttca gacggcattg caaaacacca ttgatttgct gaaagcacaa ggcgcggaac
     8521 cggtcgaagt ttccctgccg caaaccaagc tgtccatccc cgcctactac gtcctcgcct
     8581 ccgcagaagc cggcaccaac ctttcacgtt acgacggcgt acgttacgga caccgtgccg
     8641 cccaattcgg cgatttggaa gaaatgtacg gcaaaacccg cgccgaaggt tttggcagcg
     8701 aagtcaaacg ccgcatcatg atcggcactt acgtactgtc gcacggctac tacgatgcct
     8761 attatctcaa agcccaaaaa ctgcgccgcc tcgttgccga tgactttcag acggcatttg
     8821 cacggtgcga cctcatcctc gcgccgaccg caccctccgc cgcacccaaa atcggagcgg
     8881 atacttctcc ggttgaaacc tacttgagcg acatttacac catcgccgtc aacctcgccg
     8941 gactgcccgc attgaccctg cccgcaggct tcagcggcgg cggactgccc gtcggtgtac
     9001 agcttgtcgg caactacttc gccgaagcca aaatcctcgg tgcggcgcat caaatccaac
     9061 tcaacagcga ttggcacggc aaacgacccg aatgaagcag aaccgcacct ttaccttccc
     9121 cgattttcgc accgtttaca gctatgcacc tttatatcgg ctgcaacatt taaaatacac
     9181 attgcgaaaa tttttcggaa aaaaagaaat ttacgccttc gagcagtttg tcaacgcctc
     9241 ccctatccgt caggggctgt tcctccactg cccgcaggat gcctatccgc tgctgcgcga
     9301 atttgttgac aggcgtttta actgcaaacg ccgtttagat gcgatgacgg cagattttct
     9361 catggcggaa aaactcttcg gcacagacgt tctgtgccaa atggaagact gccgcttcca
     9421 tctggtcttg gcgcaccttt cagacggcat cagcttgtgg ctcaaccgca acgacaactg
     9481 cgtcgaagaa ggcgcgtggt ctttatcttt gcgcgatgaa gcaggcaacc ggctgtatat
     9541 ggcgaccttc gcctttgtcg gcacacacct gctgacagcc tccgtacaag ggccgtcagg
     9601 tgaagaagcc aaagacaccg tccgccgcat aaccaaacaa cttcacggct tacgtcccca
     9661 acaactgatg gtaaccgcac tgcaatattt cgccgccgca ttaaaattag acggcgcgat
     9721 aggcattgca caaaaacatc aagtcaaatt acgctggaaa cttaaaaagc gcgtcaaaat
     9781 gaattacgac gcattctggc aggaatacgg cgcaagtttg gaacgggacg gctactggca
     9841 tcttccccaa acccccgccc gcaaagacct tgccgacatc gaaagcaaaa agcgttcgat
     9901 gtaccgcaag cgttatgaaa tgctggacga tatggttaca aagatgaagg acagtctgaa
     9961 aacagaagca cgcggcattt cagacggcat ccaaacggaa aaaccgctcc gccggacagt
    10021 ctgatgcgaa gactatcgaa ttgatatttt agagaaagaa cctcctatga cttgggaaac
    10081 tgtaatcggc ttggaaatcc acgtccaatt gaacaccaaa tccaaaatct tcagcggtgc
    10141 atcgaccgca ttcggcgcag aacccaacgc gcacgccagc gtagtggaat gcgcgctgcc
    10201 gggcgtactg ccggtaatga accgcgaagt cgttgaaaaa gccatcaaat taggtttggc
    10261 tttggatgca aaaatcaacc ggaaaaacgt gttcgaccgc aaaaactact tctatcccga
    10321 cttgccgaaa ggctatcaaa tcagccagtt ggatttgccg attgtcgaac acggcaaatt
    10381 ggaaatcgtg gtcggcggcg atgtgaaaac catcaacgta acccgcgccc atatggaaga
    10441 agacgcaggc aaatccgtgc acgagggctt gaacggcgcg accggcatcg acctgaaccg
    10501 cgccggcacg ccgctgttgg aagtggtatc cgaacccgaa atgcgctccg ccgccgaagc
    10561 cgttgcctac gccaaagcct tacacagctt ggtaacctgg ctggacattt gcgacggcaa
    10621 tatggcggaa ggttcgttcc gcatcgatgc caacgtatcc gttcgcccga aaggccaagc
    10681 ggaattcggc acgcgccgcg aaattaaaaa cctcaattcc ttccgtttct tggatcaggc
    10741 gattaattac gaagccgaag cgcaaatcga gattttggaa gacggcggca cagttcagca
    10801 ggcgacgatg ctgttcgacc cggaaaaagg cgaaacccgc gtgatgcgcc tgaaagaaga
    10861 cgcgcacgac tacggctact tccccgatcc cgatttgctg cccgtcatca tttcagatgc
    10921 ccaaatgcaa aaagccaaag cagaaatgcc cgagctgccg aaagaaatgg cggcgcgttt
    10981 cgtggcggat tacggcgtgt ccgaatacga cgcgcgcctg ctcaccgcca gccgcgtgca
    11041 ggctgcctat tttgaagaag ccgccaaaga gagcggacaa ggcaagccga ctgccaactg
    11101 gatgaacggc gaacttgccg ctacgctgaa caaagaaggc atggaacttg ccgacagccc
    11161 gattaccgcc ccacgccttg ccgcgctggt gggcaaaatt gccgatggca cattaagcgg
    11221 caaactggcg aaaaaagcct ttgaagccat gtgggcggaa ccggaaacca gcattgccga
    11281 aatcatcgaa aaacacagtt tgcaacagat gaccgatacc ggcgcggttg aagcgatggt
    11341 ggacgaagtg ctggcaaaca acgccaaagc cgtggaacag tttaaatccg gcaacgaaaa
    11401 agccctgaat gcgattgtgg ggcaagtgat gaagaccagc aaaggcaaag ccaatcccgc
    11461 gcaggtgcag gagttgatta aagccaaact tgcctgataa gcaagatgcc gtctgaaacc
    11521 caaatccgac gttcagacgg cattttcaca gtctgaccac attaaaacag cataaaaaac
    11581 aatattccat gaaccacacc gttaccctgc ccgaccaaac cacctttgcc gccggcgacg
    11641 gcgaaaccgt tttgtccgct gccgcccgtc aaaacctcaa tctgccccat tcctgcaaaa
    11701 acggtgtctg cggacaatgc aaagccgaac tggccagcgg cgatattcaa atgggcggac
    11761 actcggaaca ggctttatcc gaagcagaaa aagcgcaagg caagattttg atgtgccgca
    11821 ccaccgcgca aagcgatatc aacatcaaca tccccggctg caaagccgat gccctacccg
    11881 tccgcaccct gcccgcacgc atcgaaagta tggttttcaa acacgatgtc gccctcctga
    11941 aacttgccct gcccaaagcc ccgccgtttg ccttctacgc cgggcaatac attgatttac
    12001 tgctgccggg caacgtcagc cgcagctact ccatcgccaa ttcgcccgac caagaaggca
    12061 ttttggaact acacatccgc aggcgcgaaa acggtgtctg ctcggaaatg attttcggca
    12121 gcgaacccaa agtcaaagaa aaaggcatcg tccgcgttaa aggcccgctc ggttcgttta
    12181 ccttgcagga agacagcggc aaacccgtca tcctgccggc aaccgacaca ggctacgccc
    12241 ccatccgcag catcctgctc gaccttatcc gccaaaacag cagccgcgct gcccatttct
    12301 actggggcgc gcgtcatcaa gatgatttgt atgccctcga agaagcacaa gggttggcat
    12361 gccgtctgaa aaacacctgc ttcacccccg tattgtcccg tcccggagag ggctggcagg
    12421 gaagaaaagg ccacgtacaa gacatcgcgg cacaagacca ccccgacctg tcggaatacg
    12481 aagtgtttgc ctgcggctct ccggctatga ccgaacaagc aaaaaatctg tttgtgcaac
    12541 agcataagct gccggaaaac ttgtttttct ccgacgcatt cacgccgtcc gcatcataat
    12601 tcctcggtat aaagaggatt cgggctttcc gttcggaaca caaaaaactt cccgtccgta
    12661 ttttccccgt gaaaaaatgc cgtctgaaac ctgattccgg ttttcagacg gcatatgttt
    12721 tttcctgttc aaggcgacaa ccgctcgcgt atccagccgc catccagcaa acggtattgg
    12781 atgcggtcgt gcagcctgct cggcctgccc tgccaaaact caatcaaatc gggaatcaca
    12841 atatagccgc cccaatgcgg cggacgcggc acgtgcaaag gatgtttgag tccgaccgcc
    12901 gccgcctttg ccaccaatac cgccttgttc ggaatcacct cgctctgcgc gcttgcccac
    12961 gcacccaaac ggctctgata cgggcgactc tcaaaatatt cgtccgacaa cttctccgcc
    13021 agccttccga cacgcccttc cacgcgcacc tgacgctcca gctccggcca aaaaaacgtc
    13081 atcgccgcaa acggattgag ttccagcgaa cgccccttgc ggctgtggta attcgtaaag
    13141 aaaacaaaac cttctgaatt aacctccttc aaaagcacca tacggctgtt gggcctcccg
    13201 cgcccgtcga ccgccgccac attgaccgcc gtcggctcgt tgacctcggc gcgtaccgcc
    13261 tcgtccaacc accgctcgaa ctgctcgatc ggattatcgg cgcaatcggc ttccgacaat
    13321 tcccgtttgc tgtaatcttc ccgaatattg tgcaaatcca tttaccgctc ctcttatcat
    13381 ttgaaagatt ctactcccgc acgcaaaccg atttcaaccg ttgcacaaac tttgtcccca
    13441 aagccgcagc gacgatttca tccgcaaaac cgccgcatca ggtacaatat cgaaccgtcc
    13501 gaccgaggac ggcattttat caacccgtcc tgccgcacac gccgcagaag aaccgcctta
    13561 tcaggcgagt taggaaaaat gatgtccaag caacccacca gcaaacgcca atggcgcgac
    13621 ggcgcagccc cgtctgccaa gaaaaccgcc aaacctttca aaagcaaagc ccgtcccaaa
    13681 gacgaaacgc gcaaaaccgc cgcccaagct tacggacaaa aagcttcaga cggcatcaag
    13741 ctgcaaaacg cccccaaaca gcgcgccgcc aaagccaaaa aactcgtcgt ccgcaatccc
    13801 aaccaaaaaa ttatggaaca cgcgcgcgat ttgaaagaac gccgcagcga cctgtcgcgt
    13861 atggaacccg aacgcctgca aaaagtgctt gcagcctccg gcgtcggctc gcgccgcgaa
    13921 atggaagaat ggatcaacaa cggctgggta acggtcaacg gcaaaaccgc gcaactgggc
    13981 gacaaagtta cccccgacga ccacgttacc gtcaaaggca gcatcatcaa actcaaatgg
    14041 gccgaccgcc tgccgcgcat catcctgtat tacaaacaag aaggcgaaat cgtttcccgc
    14101 gacgacccgc aaggccgcgt cagcatattc gaccgcctgc cgcaggccgc cagcagccgc
    14161 tgggtcgcca tcggacgctt ggacatcaac accagcggac ttctgattct cactacctcc
    14221 ggcgaactcg tccaacgttt cgcccacccc agcttcgaag tcgaacgcga atacgccgtg
    14281 cgcgtactgg gcgggctgac taccgaacaa atgcgcagtc tcaccgaaga aggcgtgatg
    14341 ctcgaagacg gcttggcaaa agtcgaacgc atctacgaac aaggcggcga aggcgcaaac
    14401 aaatggtaca acatcgtgat taaagaaggc cgcaaccgcg aagtgcgccg catattcgaa
    14461 agccaaggcc tcacagtcag ccgcctcgtg cgcgtcgcct tcggccccat cggactgccc
    14521 aaccgcctca aacgcggaca gttctacgaa ctcaaccccg ccgaagtcgc caacatcctc
    14581 aaatgggcgg atatgctgct gccgggcgaa cgccgccgca aaaaagcctg aaagtaaaaa
    14641 tgccgtctga aacacctgct gtttcagacg gcattttatt cgggcgtttt caggagaaaa
    14701 ggtcgagtgc tttgacaaag accatcacca cgccgtaggc gagcggtatg acggccaatg
    14761 cccaacgcca ccagaccgat atgccgtcgc cggaaacttt ttcacccaca aggtaagcgt
    14821 cggatatggc ggtttcgtcg tcggggttgc cgctgcgtgc ggcggttttg atgtcttttt
    14881 cgtggtgttt ttcgtgtacg gatttgacgg cgaggttgca caacagtccg acaatcaaca
    14941 atcccgccat aatgtacatg gttacgctgt atgcctgtgc cgccggtatg ccgctgtcga
    15001 tttggctttg gcgtatgtaa ttgaccagta ccgggccgat gacggcggcg gttgaccagg
    15061 ccagcaggat gcgcccgtga atcgcgccga cctgataggt gccgaacagg tctttcaggt
    15121 aggcgggaat ggcggcaaat ccgccgccgt acatggaaat aatcacgcag aagccgatga
    15181 tgaacagggc tttgctgccg ccttcgccga tggagggaac ggcgaaatac agcagcgaac
    15241 cgagtacgaa gaagatggtg taggtgtttt tgcgtccgat tttgtcggaa acgctcgacc
    15301 acaaaaagcg tccgcccata ttaaacaggc tcaggaggct gacgaagcct gccgccgcac
    15361 ctgcgccgac tgccgcctgc ctgcctgcgg aggtttcgga aaagagttcc tgaatcatca
    15421 cggatgcctg gcccaatacg ccgatgccgg cggttacgtt caggcacaat acccagaaca
    15481 acagccaaaa ctgcggcgtt ttcatggctt gggacacgtt gacatgattg ctgctgaccg
    15541 gcttgttttg cgttttcggc acggtatagc cttcaggttt ccagccgtcg gcaggtacgc
    15601 ggatggtaaa cgcgccgaac atcatcagtg cgaggtaaaa caggcccaat acggcaaagg
    15661 tttcggcaac cccgaccgaa gcggcgtttg aaaaggcgtt catcagtgat acggaaagcg
    15721 gcgaggccag cattgcgccg ccgccgaaac ccataatcgc caaaccggtc gccatacccg
    15781 gcttgtcggg aaaccatttc atcagcgtgg aaaccggccc gacgtagccc aaacccaagc
    15841 ctacgccgcc gatgacgccg ttgcccaaat aaagcaggaa gaggttgtgc gtacgcacgc
    15901 cgaatgcgga tacgaagaag cccaggctga aacagcaggc ggcggcaaat atggctttgc
    15961 gcggccctac ccgttccatc catgtgccga acagggcggc cgacgcgccc agcatcgcga
    16021 gtgcgatact gaaaatccaa cctacggtcg tcagcttcca atctccggcc gccgattcgg
    16081 ttatgccgat aagtttggtc agcggcgcgt tgaatacgga ataggcgtaa atctgcccga
    16141 tggcaaggtg caccgccaat gctgcgggcg gtacgagcca acggttgaaa cccggcttgg
    16201 caatgcttgc ctcacggtct aaaaacttca taacatcctc tttctgtcag ttgaa
//
LOCUS       DAWTHD010000052        16203 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWTHD010000052 DAWTHD010000000
VERSION     DAWTHD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.052"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..143)
                     /locus_tag="WM704_001225"
     CDS             complement(<1..143)
                     /locus_tag="WM704_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340645.1"
                     /translation="MPSERLSDGIFPCILNRVYETPDAFGGNVGCCAMFGGDQRTKCA
                     GKDL"
     gene            complement(161..718)
                     /locus_tag="WM704_001226"
     CDS             complement(161..718)
                     /locus_tag="WM704_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFA9340646.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(793..2427)
                     /locus_tag="WM704_001227"
     CDS             complement(793..2427)
                     /locus_tag="WM704_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HFA9340647.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2828..3934)
                     /gene="serC"
                     /locus_tag="WM704_001228"
     CDS             complement(2828..3934)
                     /gene="serC"
                     /locus_tag="WM704_001228"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFA9340648.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYQIVPKLSDGLPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4300..4731
                     /gene="rimP"
                     /locus_tag="WM704_001229"
     CDS             4300..4731
                     /gene="rimP"
                     /locus_tag="WM704_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFA9340649.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4759..6261
                     /gene="nusA"
                     /locus_tag="WM704_001230"
     CDS             4759..6261
                     /gene="nusA"
                     /locus_tag="WM704_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HFA9340650.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6273..9104
                     /gene="infB"
                     /locus_tag="WM704_001231"
     CDS             6273..9104
                     /gene="infB"
                     /locus_tag="WM704_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFA9340651.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9264..10604)
                     /locus_tag="WM704_001232"
     CDS             complement(9264..10604)
                     /locus_tag="WM704_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFA9340652.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GGARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10678..12018)
                     /locus_tag="WM704_001233"
     CDS             complement(10678..12018)
                     /locus_tag="WM704_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFA9340653.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRSEMIDRDRMLRDTLERVRAGSFWLWVVVASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12161..12787
                     /locus_tag="WM704_001234"
     CDS             12161..12787
                     /locus_tag="WM704_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFA9340654.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12852..14270)
                     /locus_tag="WM704_001235"
     CDS             complement(12852..14270)
                     /locus_tag="WM704_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9340655.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKQ"
     gene            14597..15325
                     /locus_tag="WM704_001236"
     CDS             14597..15325
                     /locus_tag="WM704_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFA9340656.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIGLRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRTQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15384..15872)
                     /locus_tag="WM704_001237"
     CDS             complement(15384..15872)
                     /locus_tag="WM704_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFA9340657.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4249 a         4630 c         4135 g         3189 t
ORIGIN      
        1 aggtctttgc ctgcgcactt ggtgcgctgg tcgcctccga acatggcgca acacccgaca
       61 tttccgccga acgcatcggg cgtttcataa acccggttta aaatgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg cattttgttt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tcctcgcaac cgaaagcctc ccgcaacaat gggctgttga
      301 cccacatccc cgtaatccag acattgtcga atccctgagc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cacaaacgtt accccgatga ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca gttcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatgttgc
      601 cgtgatcgga aacttgggtt gccgcctgca atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttt cggacacata tgccgtgaag gcttcagacg
      781 gcatatccgg catcagcgcg gacggcggca ggctgccaat atatccattt ccttccgata
      841 ggtttggctg ttggaaatgt ccatcagccc caataccgtg ctgaaatagt ggtcgtgcga
      901 atattcgttt tccgccgctt tttgtttgag gcattggaaa tctatgcccc cgtgttggcg
      961 gaaggctttg gaaaaccaca taaccatcgg gatatgcgtc tgtccggaag gcgcgatggc
     1021 gtaaggcgcg gcgtgcaggt acatcccgtt ttcgcccaaa ctttcgccgt ggtcggaaac
     1081 ataatacacc gcgctttcca aatcgtcgcg gttttcaagt ttgcggataa ccttgtcgat
     1141 aaactgatcc acatacaaaa ccgtattgtc gtaagtgttg accaacgtgg cgcgggcgca
     1201 tttgtcgatt tcgttggtat cgcatgtcgg cgtgaatttg cgctcggctt cggtgtagcg
     1261 ttcgtaatac gtcggcccgt ggctgccgat ggtatgcagg attaaaaccg cgtctttatc
     1321 gtttttgttg agggcttcgt cgaacttggt caacaggata ttgtcgaggc actcgccgtt
     1381 gcggcagtat tcgggcaggt tgagcgaagt aacgtcggta ttcggcactt tgccgcacac
     1441 gcccttgcag ccggaatcgt tttccaacca agtaacttcc acgccggcgc gctgcacgat
     1501 gtccagcagg ttgtcttggt gttcggcttt gatttcgtca taatccgtgc ggtcgaaggt
     1561 tgagaacata cacggcaggg agtgcgcggt cgatgtgccg cagcttctga cctgcgggaa
     1621 attgacaatt tcatcgccgc gcgcggcaag cagcggcgta gtttggcggc tgtaaccgtt
     1681 caaaccccag ttggcggcac gcgtagtctc gcccacgacc agcaccacga aacggcgcag
     1741 gctgccggcc ggccggtttt gcacgaccgc catatccaat tgcgtataag gaatattgga
     1801 acgcttccaa tctttgtatt tcgacacgcc cgcgccgatg aaattagacg gcacaatcag
     1861 atgggttact gatttattgt tgcggaaaaa cgaggcgtaa tcctgatatt gcaacatcgc
     1921 gatgcccaac gcgcacaaaa aggaaacggc ggcaagcaca aggcgcgtca aaagctcctt
     1981 ataccaaacg cggtatttga ccttgacggc gatatatgcc agcgcgggca atacgcccaa
     2041 acatacaatc cacagcacat agcccggcgt aatcaggcgc gcgctttccg ccgccgtggt
     2101 ttgcaagaca ttattcaaca tcgacttgtt gaaatagata ttgaaaaata tttcttggta
     2161 agacaccgcc gcactgataa ccaatatcaa tggaatcaac actttatgca caaaaggcag
     2221 ggcaatgacg tgaaaaacga aattacttaa aaaaaacagc accaccggca tcgtatagag
     2281 gaagatgtcc gccccggtgt cgttaaaagg gcgaagttcg acaactttgg caaaaaaggc
     2341 gtaattcaat accagcgaag aatacaggga aagaaaggca atcagcgcgg aagagccgag
     2401 cttcggcctc aggttcggtt ttatcatttg gaatgtgtcg gataagggtt ggaaaaggca
     2461 tccggaatcc ggattattga aaaagattct taattataag gcaacggagc aaagcagggc
     2521 aagaaaacga gtaggcggcg cgggggtttc cgcccgccat tcaaacgtcc ggcagacata
     2581 aaaacatcgt aagcaaaatt cgaaccggtc tgcaaccgcc cctgccggaa aagcgggcaa
     2641 agcatttcat attggaaaaa cccggacgcg ccgccgacgg gacagtccgg cacaaacagc
     2701 atcacgcccg gattgaaaag gacggatagc cgccggcagc gcagttttac cccgcatccc
     2761 caaccgccgc cggagaacgc cgaaacagcc gatgccgtct gaaacacatt cgggcggcat
     2821 ttgaaaatca accgtaacgc cgttggaaat cgcgcataaa atctgccaaa gcccgcacgc
     2881 cttcaagcgg catcgcatta taaatgctgg cgcgcatccc gccgacagac ttatagccct
     2941 tcaacaggca caagccctgc aactcggctt ccaacacaaa acggcggtca agctcctcat
     3001 cccccgtttg gaacacgaca ttcattttag aacgcgcatt cggacggata cggttgatat
     3061 agaaaccgcc gctgccatct atcgtctcat acaaggtttg cgccttcagc cgattgaccg
     3121 cttcaatttt tttcacaccg ccctgcgcct gcagccagcg gaacaccagc cccgacatat
     3181 aaatcgcgta agttgacggc gtgttgtaca taccgtcgcg gttcaaatga gaacggtagt
     3241 tgaacacatc gggaatatcg ttcggacaac gctcgagcaa atcctcacgc acaatcacca
     3301 ccgtaactcc tgccggcccg atgtttttct gtgcgcctgc gtaaatcagt ccgtagtcgg
     3361 caacatcaaa ctcgcgcgac agaatctcgc tggacatatc gcacaccaac ggcggcaggc
     3421 cgtctgaaag cttggggacg atttggtatt gcaggccgtt gacagtttca ttgacggcaa
     3481 aatggacaaa cgccgaatcg ggtgcaacat cccacgtttc cacaggcggc aggtcaagat
     3541 agtcgaactg ctcgcccccg tgcgccgcca aacggatttc cgtatcggtc aaacggctca
     3601 tctgttcata ggcgatacgg ctccagttgc ccgttaccac cgcgtcggca gtgcggaaac
     3661 cgtgtgccag attcatggct gccatattaa attgggttgt tgctccgccc tgaagaaaca
     3721 atatcttata gttgtcaggc actttcaaaa gctgcctcaa atcctgttcc gcatgatgca
     3781 ggatgctcaa aaacatttcc gaacggtggc tcatttccat cacggaaaaa cccgtaccgt
     3841 tgtagtccga catttcctgc tgcgccgtcc tcaacacagc ttcaggcaac acggcagggc
     3901 cggcggaaaa attgtaaatc ggataaagag acatgatgca gccctggttc tgaacaataa
     3961 cccgcccgat tttaggcttg cagcaggctt tgtgcaagga tgggaaatac ctgtcctccg
     4021 cccgattcca tgccgcccga acacggaaaa taatatcaat atattgattt acaaacataa
     4081 aaatcatgct agcgacaaat agatacattt gttttgtcaa caatattcac gatttcccat
     4141 tacaaacctc ccgtacaccc gcttttttcc gtcccaaaaa cacaaaataa atcaacactt
     4201 tcatttctcc gcaaaagcgg ttataatcac gccgattttt caacttttga cgaaaaatgg
     4261 ccacggggcc atttttttat tatgtacata gggagcagca tggatattca aaccatcctc
     4321 gaaaaaaccc tgcccggcct gggatacgaa ctggtcgatt tcgaactggc cgcgcaagga
     4381 acattgcgcg tgttcatcga caaagaaggc ggcattaccg tcgaagactg cgccaccgtc
     4441 agcaaccact tgagccgcgt cttcatggtt gaagacatcg gctacaaaaa cctggaaatt
     4501 tccagccccg gactcgaccg ccccttgaaa aaagccgccg acttcgtgcg ctttgccggt
     4561 cagaatgcca aaatcaaaac ccgcctgccg ataggcggtc agaaaaactt tatcggtaaa
     4621 atcgaaggct gcgaaaacga cactgttacc gtttccttcg acggcaaaac cgtgcaaatc
     4681 gaattgggca acatcgacaa agcccgcctg cgccccgaat tcaaattcta aaacacagca
     4741 atattggaga tgttcaaaat gagtcgtgaa atgttacagc tggcagaagc actggcaagc
     4801 gaaaaaaacg ttgatgcgga agtcgtcttc caagcactgg aattcgcact ctctaccgcc
     4861 gccaagaaaa aggcagaccg cgaacacatg gacgtgcgcg tccaaatcaa ccgcgatacc
     4921 ggcgaatacc aaaccttccg ccgctggctg attgtggccg atgaagacta tacctatccc
     4981 gatgtcgaaa aaaccatcga ggaaatccaa gaggaaatcc ccgatacgac catccaaatc
     5041 ggcgaatact acgaagagca gctgcccaac gaaggcttcg gccgccaagc ggcgcaaact
     5101 gccaaacaaa tcatcctgca acgcatccgc gatgccgagc gcgagcagaa tctgaacgag
     5161 ttcctcgccg tcaaagaaga catcgtgtcc ggcacggtca aacgcgtcga acgccacggc
     5221 atcatcgtcg aagtcgttgc cggcaaactg gacgcgctga ttccgcgcga ccaaatgatt
     5281 ccgcgcgaaa acttccgcag cggcgaccgc atccgcgccc tcttcctgcg cgtcgaagaa
     5341 atcggcaaca ccggccgcaa acaagtcatc ctgagccgta cttccggcga tttcctcgtc
     5401 aaactgtacg ccaatgaagt acctgaaatt gcaggcggca tgcttgaaat ccgcgctgtc
     5461 gcccgcgacc cgggccaacg tgccaaagtc gccgtcaaag ccaacgacca gcgcatcgat
     5521 ccgcaaggca cctgtatcgg cgttcgcggt tcgcgtgtca atgccgtcag caatgaattg
     5581 tccggcgagc gcatcgacgt tgtcctttgg tcgcccgagc ctgcccaatt cgtcatgagc
     5641 gcgctctcac ccgccgaagt cagccgcatc gtcatcgacg aagacaaaca cgccgtcgat
     5701 gtcatcgttg ccgaggaccg gctcgcgctc gccatcggac gcggcggtca aaacgtgcgc
     5761 cttgcttccg acctgaccgg ctggcagctc aacatcatga cttccgccga agcagacgaa
     5821 cgcaatgcgg cagaagatgc cgccatccgc cgcctgttta tgaatcactt gaatgtagat
     5881 gaagaaaccg ccgacgtact ggttcaagaa ggttttgcaa ccttggaaga agtcgcctat
     5941 gttcctgccg ccgaactgct tgccattgaa ggatttgacg aagaaatcgt cgatatgctc
     6001 cgcaaccgtg cccgcgatgc catcctgacc atggcgattg ctgccgaaga aaaactgggc
     6061 gaagtgtccg acgatatgcg taacctcgaa ggcgtagatg ccgatatgct cctcagcctt
     6121 gccgaagcag gcattaccac ccgcgacgac ttggcagagc ttgccgtgga cgaactgatt
     6181 gaaatcaccg gtgtaaacga agaaactgca aaagccgtca tcctgaccgc acgcgaacac
     6241 tggtttaccg aagacaaata aagggggtac agatgagtaa cacaaccgta gaacaatttg
     6301 ccgccgagct gaaacgcccc gtcgaagacc tgttgaaaca gttgaaagaa gccggcgtca
     6361 gcaaaaacag cggcagcgat tccctgacgc tggacgacaa acagcttctg aacgcctacc
     6421 tgaccaagaa aaacggcagc aacggcggca ccatcagcat ccgccgcacc aaaaccgaag
     6481 tcagcaccgt tgacggcgta aaagtcgaaa cacgcaaacg cggacgcact gtcaacattc
     6541 cttctgccga agaattggca gcacaagtaa aagccgccca aacccaagcc gcacctgttc
     6601 agccggagca gacggcagaa gacgcggtaa aagcccgagc cgaagctgcc gcacgcgcag
     6661 aagcccgtgc caaggcagaa gcggaagcgg caaaactgaa agcggcaaaa gcaggcaaca
     6721 aagccaaacc tgccgcgcag aaacctaccg aagcaaaagc cgaaaccgca cccgttgcgg
     6781 cggaaaccaa acccgccgag cccaaagaaa aagccgtcaa gccgaaacac gagcgaaacg
     6841 gcaaaggcaa agatgccaaa aaaccggcga aacctgccgc acctgccgtg ccgcaacccg
     6901 tggtcagcgc ggaagaacag gcgcaacgcg acgaagaggc gcgccgcgcc gccgcattgc
     6961 gcgcccacca ggaagccctg ttgaaagaga aacaggaacg ccaggcacgt cgcgaagcca
     7021 tgaaacaaca ggcagaacaa caggcaaaag ccgcacagga agccaaaacc ggcagacagc
     7081 gtcccgccaa acctgccgag aaaccgcagg cagccgcacc ggccgtcgaa aacaaacctg
     7141 tcaatccggc aaaagcgaaa aaagaagacc gccgcaaccg cgatgacgaa ggtcaaggcc
     7201 gcaacgccaa aggcaaaggt gcaaaaggcg ggcgtgaccg caacaatgcc cgcaacggcg
     7261 gcgacgagcg cgtacgcggc ggcaagaaag gcaaaaaact caaactcgag ccgaaccaac
     7321 acgccttcca agcaccgacc gaacccgtcg tacacgaggt tttggttccc gaaaccatta
     7381 ccgttgccga tttggcgcac aaaatggcgg tcaaaggcgt ggaagtggtc aaagccctga
     7441 tgaagatggg catgatggtt accatcaacc aatccatcga ccaagacacc gccctgattg
     7501 tcgtcgaaga actcggacac atcggcaaac ccgccgccgc cgacgatccc gaagccttct
     7561 tgggcgaggg cgcggaagcg gtggaagccg aagcattgcc gcgtccgccc gtcgttaccg
     7621 tcatgggcca cgtcgaccac ggcaaaacct cgctgctgga ctacatccgc cgcgccaaag
     7681 tggtacaggg cgaagcgggc ggcattacgc agcacatcgg cgcgtaccac gtcaaaaccc
     7741 cgcgcggcgt gattaccttc ttggacaccc cgggccacga agcctttacc gctatgcgcg
     7801 cgcgcggcgc gaaagcaacc gatatcgtga ttcttgtggt cgccgccgac gacggcgtga
     7861 tgccgcaaac catcgaggca attgcccacg ctaaagcggc aggcgttccg attgtggttg
     7921 ccgtcaataa aatcgataaa gacactgcca accccgaacg catccgtcag gaactgaccc
     7981 aacacgaagt catccccgac gattggggcg gcacggttca attcatcgac gtttccgcga
     8041 aaaaaggaac gaacatcgac gcgctgctcg aagccgtatt gctcgaagcc gaagtattgg
     8101 aactgaccgc acctgtcgat gcacccgcca aaggcatcat cgtcgaggcg cgtttggaca
     8161 aagggcgcgg cgcggttgcc acattgctgg ttcaaaacgg tacgctgaaa aaaggcgaca
     8221 tgctgctggc cggtacggca ttcggcaaaa tccgcgcgat ggtcgatgaa aacggcaaat
     8281 ccattaccga agccggcccg tccatccccg tcgaaatcct cggcttgtcc gacgtaccga
     8341 atgcgggtga agacgcgatg gtattggcgg acgagaaaaa agcgcgcgaa atcgccctct
     8401 tccgccaagg caaataccgc gacgtgcgcc ttgccaaaca gcaggcggcg aagctggaaa
     8461 atatgttcaa caatatgggc gaaacccagg cccaatcttt gtcggtcatc atcaaggcag
     8521 acgttcaggg ctcttacgag gctttggcgg gcagcctgaa aaaactgtcc gccgacgagg
     8581 tgaaagtgaa cgtgttgcac agcggcgtgg gcggcattac cgaatcggat gtcaaccttg
     8641 ccatcgcttc gggcgcgttc attatcggct ttaacgtgcg tgcagatgcc tcttcgcgca
     8701 aacttgccga aaatgaaaac gtggaaatcc gctactacaa catcatctac gatgccatcg
     8761 acgacgtgaa ggcggcgatg agcggcatgc tttctcccga aaagaaagaa caggttaccg
     8821 gtacggtcga aatccgtcag gtcatctccg tttccaaggt cggcaacatt gcaggctgta
     8881 tggtcaccga cggcgtggtc aaacgcgatt cccatatccg cctcatccgc aacaacgtgg
     8941 tcatccacac gggcgaactg gcttcgttga aacgctataa agacgacgta aaagaagtcc
     9001 gcatgggctt cgagtgcggt ctgatgctca aaggctacaa cgaaatcatg gaaggcgacc
     9061 aactggaatg cttcgacatc gtcgaagttg cccgcaccct gtaattcctt tgcaaataaa
     9121 atgccgtctg aagcgttcag acggcaacga aacgggttct gtatcataca gaacccgttt
     9181 tttgtcgcaa atcggcttca gcgaccctcc tgccttatcc cgatttgaat ctgacttgcc
     9241 atacaaacag gcttcagacg gcattatttg cccgccaaac gtatcccgat cttctccgca
     9301 tattccctgc gttcggcgcg gctggtttcc gggcggtgcg tattgagcga cgaccatttc
     9361 caatgactgc gggctttgtt gagttcgggc gggagtttgg cggcatccca cggggctttg
     9421 cggctgtgca gctcgatatc cgactgtgcc gcgtgtccgc gcgtttgcag gacgtggagc
     9481 agatcgaggg cgcgggcggc gagcagggtc agggtttcag ggtcggtgtg cagggtttga
     9541 cggccggcga gtttgtcgga aatggtgcgg gtattgggca ggatgccgcc caaaaagccg
     9601 ccgattgccg tacccaatcc gagcgagccg ccgagtgtgg cgatgtccag ccccaagccg
     9661 atgagcgcgc cggttgccgc gcccgtgccg gtgcggatgc cgtattgttt gagcaattcg
     9721 ctgtcgaacg ggtcttggcg gaaggcttgc ggcatccagt cgccgccgtc gatttcgctg
     9781 tggtagaaac ggtaaagggc aaacagccgc tgctgcatct gccgttcgag ttggcgtatt
     9841 tccgcctgca tggtttgcag cacggtggcg gtatcctcgt tttcgtccac ttcctgcctg
     9901 aaggcggcgg catcgagcag gaaatcggcg atttcgcggc gcgctccgcc gtccagccgc
     9961 tgccattcgc gccggcgcat ggctgtcagg cggtcaagtg tgctgcgttc gggcaacatg
    10021 gtggcgaggt tttcccacag gcgcaattcg ccttcaaaat caaaggcgac ggtgtcgaac
    10081 cctgcgaaaa cgtgcaggtt tctcctcgcc agcatggttg tccacgattc gggaagctgt
    10141 ccgccggtaa agttgaacac gggcataacc ggtttggcac accatgaaag gatggtcaat
    10201 tcatcccgat atttgtcgag gacgggttcg cgcgcgtcga tgacgtacat tgccatatcg
    10261 ctttgcaaaa cctgccgcag cactttggct tcttggttga aatcgtgatg cgcgccgtgg
    10321 ctgccgagaa actgttgcag ccgctcgatg ccgtctgaac gattgtccgt atggttttcc
    10381 agccattcca aaacaccgcc cgcgtcttcg agtccgggcg tgtcatacag gaaaaccaac
    10441 atgtccgcgc cgtcgctgat ggcggcttct tcgacatggc gtgtggtgga cggtgcgttt
    10501 ctgacttcgc cgaagccgct gtcgcgcaac agggtgcgca ggagcgaggt tttgccggtg
    10561 ttggtgtgtc ccacgacggc gagggaaagg ggttgtttgt tcatgatgtt tttgaagaat
    10621 ggattttcag acggtctttt ttcagaatgg cggcttaaca gaacatttca agtgagttta
    10681 ttggtctttc aaacggcctt cctgcgccac cctgtcaggc tcaagccacg ccgcgccgca
    10741 ttcggtcagc gcgttacgcc aatgttccag cttttccgaa aggtcgtctg aaagcccctg
    10801 ttccgccaaa agctgcacca ccgcgccgcc ctgcgccgct tccgaaagcc gcacaatctg
    10861 ccgcagcacg ccccggtccg gcacagtttg ggcgcgtacg ccgataagca gttgcgccgg
    10921 tttctgcttc agctctgtct ccagcgcggc aacctgttcc cgattggcgg caacgccctt
    10981 atccagccat tcctgcgcca gcctgccctc gaaccattgg ccgtcctgcc actcggtctc
    11041 cagcatgagc gcccatttcg gcgcatcgtt caagacgatt ttcggcgaaa cggcggacac
    11101 ggtttcccga cgcgtatccg catcggtgat tttgttctgc cagcggcgga tgaccgcctg
    11161 ataataggtt ttttccaaat ccaatccgtt ttcgcttgtt ttcaaaagga ttttacacac
    11221 tacccaagcc aagaggcgcg gcaggatgcc gtagcagacg atactgccga ccagcagccc
    11281 cgaccaagcc cgcgcatcgg caatattgcc gttcagacga ccttcgatga ccgcccgcgc
    11341 atcggggaca gggaaaccga gtttcgacgg cagccatgcc aacatttcca ccgcgcgtac
    11401 cgaagcggca ttgctcaaca gcgtgctttc ccagttgaac gtatattgcc gcaccaaaag
    11461 cagcagcaat accgacacca gcattccgag cagcgtgcag agccacaagc tgtgcgccgt
    11521 tgcgcctatt ttccatcgta ccgaaggttg ccgccactgg tccgcataca gccgcaacac
    11581 cgcctgattt acagggcctt tgccccgaaa ccacgtcgcc ggactgctga aaaaccgccc
    11641 cactttcacg cgcaggaaca acgttgccaa ccatactgcc agcatcagcg tattcatgcc
    11701 caacactccc gccaaaacta aaaagaaatt cagcccctga ttgtccatca gaagataagt
    11761 gcctgaaaat ccggcggtaa acatcatcga tgccaccacc acccataacc agaacgaccc
    11821 cgcacgcaca cgttccaacg tgtcccgcag catacggtcc ctgtcgatca tctccgaccg
    11881 acggatgatt ttttcctccg tactgccgtc cacgcggcgc aaagcctccg tcgcctgcac
    11941 gggatcgccg ctgaaaataa aaccgccttc atccaaaata cggaccagct caaccagttt
    12001 tcgggatgga ttcaacataa aatgccgtct gaaaataaaa aacaaatttt aacacacgca
    12061 ttttcaagaa tattcacagt gtaggcaaag agtaaatccc acacagaggc aaaagtatcg
    12121 gcgtaaactg actgcctcta ctttcccgaa agattgtgcg atgtatgcag gcgaacgctt
    12181 caatacttac agccatttga gcggtttgat tctggcggcg gcaggtttga tgctgatgct
    12241 gctgaaaacc ataggacacg gggacggcta ccgtatcttc agcgtatcgg tttacggcat
    12301 cagccttctt ctgctctatt tgagttcctc gctgtaccac ggaattgcag ccggaaaact
    12361 gaaaagcatt ttgaaaaaaa ccgaccactg catgatttat gtgctgattg ccggaagcta
    12421 cacaccgttt gcactggttt ctttgagaaa cgggccgggc tggacggtat tttcactgtc
    12481 ctggctgctg gcggctgcag gaatcgcaca agaactcacc atcggacgga aaagcgaaaa
    12541 acgtctgctg tctattgcga tttatatcgt aatgggctgg atggtcttgg cggtaatgaa
    12601 atccctgaca gcctcactcc cgccggcagg actggcttgg ctggcggcag gcggtatgct
    12661 gtacagcgtc ggcatttact ggtttgtaaa cgatgaaaaa atccgacacg ggcacggaat
    12721 ctggcatctg ttcgtattgg gcggcagcat aacccaattt gtcagcgtgt acggttatgt
    12781 aatctgaatg ccgtctgaaa cacaaaaacc tcccaaacct gaaggcaggg aggttttctc
    12841 tttgccgggc attattgttt atcgtggaac tcgtggaatt catactgata ggacaaatcc
    12901 cgacccgctt ttttctgtgc caaataatca tcataaatgg cgcggatttc cttacgcagc
    12961 aagaacaggg caatcaggtt ggggataacc ataaagccgt tgaacatatc ggacagattc
    13021 caaaccaaat cgactttgcc gagcgtaccc aaaacgatag caagcagaac caatgcgcga
    13081 tagatgccca agtgtcttcc cctgaaaaga aaacggatgt tggactcgcc gaaataatac
    13141 cagccgataa tggtcgtaaa ggcgaagaag gtcagacaca cggcaagcaa ttgcgaaccg
    13201 aagcccggaa atgccttgtt aaaggcaaat tgagtaaccg ccgcgccctg ttcgcccgaa
    13261 aggttggcat cggtcagcag gataatcaat gccgtagccg tacataccaa aatcgtatcg
    13321 ataaatacac cgacaaatgc cgtcatacct tgctgaacag gatgcttcac atccgcagtc
    13381 gcgtgggcgt gcggagtcga acccatacct gcttcgttgg aaaacagacc gcgcgccacg
    13441 ccgaaacgta tcgcttcacg cataccgata cccgcagcac cgcccaaaac ggcctcggga
    13501 ttgaaggcgg cggtaaagat gtggttgaac atcggcacaa tatggtcgga aaattcaaac
    13561 aggataacga cggcgcacaa aatataaaca accgccataa acggcacgac aaaccgggca
    13621 atattggcaa tacggttcac gccgccaatc acaaccatgc ccgcaaggac ggcaaacaca
    13681 ataccgactg ccaaagaagg cacatcaaat gcaatggtaa cggcagaagc gatggagttt
    13741 gcctgagtcg cattgccgat aaagcccaac gcgacaatca gcgcgatgga gaaaaagccc
    13801 gacaggaaac gcgccgcgcc cctgccgatt ttcggagtca gaccgtgcgt aatataaaac
    13861 gccggcccgc cgatgtattt gccgtggctg acgacgcggt atttctgcgc cagcagtgct
    13921 tccgcaaaaa tcgtggacat ccccaaaacg gcagaaagcc acatccaaaa aatcgcaccc
    13981 ggcccgcctg cggtaatggc ggtcgccaca ccggcgacgt tgcccgtacc gatttgcgcg
    14041 gatatagcaa ccgccaacgc ctgaaactgc gataaggact tatcgtcttt atcacctttg
    14101 gcaaacaagc cgccgaatac ggatttgaat cccgcgccca gcttggtaat ctgcggcgca
    14161 ccaagataca gcgtaaaaaa caggccgata cccaaaagcg cataaatcag caggtagccc
    14221 caaagaaacc gattgactgt acccaccaga acagacaata tattttccat aaaataaacc
    14281 ttatcttacg attaaaatga ctgttttcca aaagacattc caataaggaa acacggcgag
    14341 cagaccgtat ttgccgcaac agatgcctta aattgtcaac aatccgagag aagctgtgcc
    14401 agcataccgt aaaatatcgt taattaaaat atttctttat ttttaagcgg aaagcggagg
    14461 aaatcgtttt cagacggcat cgacaacggc acggcataaa acaggatatt ttgggtactt
    14521 gcaacttatg ttaaaatgcc gaccgtaaaa aatctgacaa aaacagatta attatttgaa
    14581 ataagaaagg aaatttatgg caggccatag caagtgggcg aatatccagc ataaaaaagc
    14641 ccgtcaggat gccaaacgcg gcaaaatctt cacccgttta atcaaagaaa tcaccgttgc
    14701 ggcgcgtatg ggcggcggcg atcccggcgc aaatccgcgc ctgcgtctgg ctttggaaaa
    14761 agcagccgaa aacaatatgc ccaaagacaa tgtgcaacgc gccatcgaca aaggtacggg
    14821 taacttggaa ggcgtggaat acatcgggtt gcgctacgaa ggctacggca tcggcggcgc
    14881 agctttgatg gtggactgcc tgaccgacaa caaaacccgc accgttgcgg acgtacgcca
    14941 cgcatttacc aaaaacggcg gcaacttggg taccgacggc tgcgtggcgt tcaacttcgt
    15001 gcatcagggc tatttggtat tcgaacccgg cgttgacgaa gacgagctga tggaagcggc
    15061 tttggaagcc ggtgcggaag acgtggttac caacgacgac ggttccatcg aagtcattac
    15121 cgcgccaaat gattgggcgg gcgtaaaatc cgctttggag gcggcaggtt acaaatccgt
    15181 tgacggcgac gttacgatgc gcacccaaaa cgaaaccgaa ctctccggcg acgatgccgt
    15241 caaaatgcaa aaactgattg acgcgctgga agacttggac gacgtgcaag acgtttacac
    15301 ttccgccgta ttgaatctgg actgatacgc agcacagcag acatacaaca aaatgccgtc
    15361 tgaacctttc agacggcatt gatttattta gcccttgccc acgcccacca aaccgtcagg
    15421 acaaccgcca aaaaatacgc cgcgctccaa acaggcaaag caacccctaa cagataatcc
    15481 ggttcggcac aatttccgaa cccgcgcaca acaggctcga accaatcaaa caaaggccag
    15541 cccttcaatc gaaacgtcca cggcgcgccg cacgaaggag ccgtacccgg cggcagcgac
    15601 tgcaaccaca actgatatgc cgcaacagaa atacccgtaa cggcgggaat actgataaac
    15661 actgcgcctg acagtccgcc caccctgccc ttgggcttac acgccagcac aactgccgca
    15721 cacaatgcgg ttgccaaaac acataaccgc tgactgatgc acaaaacgca aggctccata
    15781 cccaaaacat actgtgccgc caaagaaccg gcaaatgcac agaccgaaac ggcaaacagc
    15841 agccaaacgg cttttctaaa taacggggtc attttctcaa cacaccaatc aaaataccga
    15901 tatgccgatt ttgctggata tatcccgaga tggcaaggga cgaatcgaca ccggcacagg
    15961 tgcggacgat ttcggttttc ccgatatgca caaaacctga caaaggacaa aacggcgatt
    16021 tgtccgcaca atgcccgaaa aaatacatcc ggaggatttg aatttgaatt tcggcaaatt
    16081 tagcgaatca aaagcaacag ccgataaaaa aatcaaaagc ttatttcaat gtttcaacac
    16141 acaggacgac acataaagca ccgccctatg tgccgtcctg atttggaagg ggttacaccc
    16201 ctc
//
LOCUS       DAWTHD010000053        16005 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWTHD010000053 DAWTHD010000000
VERSION     DAWTHD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.053"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            1108..2217
                     /locus_tag="WM704_001238"
     CDS             1108..2217
                     /locus_tag="WM704_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9340658.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2946..3758)
                     /locus_tag="WM704_001239"
     CDS             complement(2946..3758)
                     /locus_tag="WM704_001239"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFA9340659.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            complement(3718..3926)
                     /locus_tag="WM704_001240"
                     /pseudo
     CDS             complement(3718..3926)
                     /locus_tag="WM704_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
     gene            3947..4408
                     /gene="tsaE"
                     /locus_tag="WM704_001241"
     CDS             3947..4408
                     /gene="tsaE"
                     /locus_tag="WM704_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFA9340660.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4405..5655
                     /locus_tag="WM704_001242"
     CDS             4405..5655
                     /locus_tag="WM704_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFA9340661.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADPAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5737..6555
                     /locus_tag="WM704_001243"
     CDS             5737..6555
                     /locus_tag="WM704_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9340662.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6601..7743)
                     /locus_tag="WM704_001244"
     CDS             complement(6601..7743)
                     /locus_tag="WM704_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFA9340663.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVAGAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNIDCRLFLIDMVQGSNR"
     gene            complement(7792..8223)
                     /locus_tag="WM704_001245"
     CDS             complement(7792..8223)
                     /locus_tag="WM704_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340664.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQQPQAVAAQCD
                     LTEGCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFER
                     QPSGTWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(8216..9025)
                     /locus_tag="WM704_001246"
     CDS             complement(8216..9025)
                     /locus_tag="WM704_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFA9340665.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVAVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(9107..9484)
                     /locus_tag="WM704_001247"
     CDS             complement(9107..9484)
                     /locus_tag="WM704_001247"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFA9340666.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9534..10262)
                     /gene="pdxJ"
                     /locus_tag="WM704_001248"
     CDS             complement(9534..10262)
                     /gene="pdxJ"
                     /locus_tag="WM704_001248"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFA9340667.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFAKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAH
                     SHAEQIRQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(10315..11058)
                     /gene="recO"
                     /locus_tag="WM704_001249"
     CDS             complement(10315..11058)
                     /gene="recO"
                     /locus_tag="WM704_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFA9340668.1"
                     /translation="MSEHRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(11092..12219)
                     /gene="pheA"
                     /locus_tag="WM704_001250"
     CDS             complement(11092..12219)
                     /gene="pheA"
                     /locus_tag="WM704_001250"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFA9340669.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIVYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIRHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(12258..13319)
                     /locus_tag="WM704_001251"
     CDS             complement(12258..13319)
                     /locus_tag="WM704_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFA9340670.1"
                     /translation="MFGTAFGQVAGGAVSDIKGRKPVALTGLIVYCLAVAAIVFASST
                     EQLLNLRAVQAFGAGMAVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLAAPMVGAL
                     LQGLGGWRAIFVFLAAYSPVLPGLVQYFLPNPAVGGKIGRDVFGLVAGRFKRVLKTRA
                     AMGYLFFQAFSFGSMFAFLTESSFVYRQLYHVTPHRYAWVFALNIITMMFFSRVTAWR
                     LKTGAHPQSILLRGIVVQFAANPSQLAAVLFFGLPPFWLPVACVMFSVGTQGLVGADT
                     QACFMSYFKEEGGSANAVSGVFRSLIGAGVGMAATVLHDGSATVMAATMTASASCGIA
                     LLWLCSHRAWKENGQSEYL"
     gene            complement(13335..13613)
                     /locus_tag="WM704_001252"
     CDS             complement(13335..13613)
                     /locus_tag="WM704_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340671.1"
                     /translation="MPSEAVSGGISFGFAPPSDMLDLRPYRPSFATVSLFDLNGKTMS
                     PPLPPMSGKLMAVLMAVLVALMPFSTDAYLPAIPEMAQPLNADIHRIE"
     gene            complement(13603..14478)
                     /locus_tag="WM704_001253"
     CDS             complement(13603..14478)
                     /locus_tag="WM704_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFA9340672.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
     gene            14630..15061
                     /locus_tag="WM704_001254"
     CDS             14630..15061
                     /locus_tag="WM704_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9340673.1"
                     /translation="MLPLFQGPDSRLRGNDEAILLAEGQKSAVTEYYLNHGIWPENND
                     SAGVASASKIIGKYVKSVTVTNGVVTAQMKPSGVNNEIKDKKLSLWAKRQDGSVKWFC
                     GQPVKRDAGAKTGADAVTADKDKEIETKHLPSTCRDTSSAE"
     gene            15606..>16005
                     /locus_tag="WM704_001255"
     CDS             15606..>16005
                     /locus_tag="WM704_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HFA9340674.1"
                     /translation="MQPAKNLLFSSLLFSSLLFSSLLFSSAAQAASEDGGRGPYVQAD
                     LAYAYEHITHDYPEPTGTKKDKISTVSDYFRNIRTRSVHPRVSVGYDFGGWRIAADYA
                     RYRKWNNSKYSVNTKNLRTHESNGNRQDLKT"
BASE COUNT         3745 a         4589 c         4255 g         3416 t
ORIGIN      
        1 ggctgttggg gttaaggttt atgcaaacta atacggcttg tgcaggctgt tttttatttt
       61 tcatacgacc tgaaatcaac ggtaaatcca agttgctttt caaaccgcct gcaaccgcaa
      121 aatctgccgc aatccgctcc ctcccccgtg ggggagggcc ggggagaggg cattctccga
      181 gttgcggcaa tctttcccaa actcccttag tcttctaaaa tacaaacctt gcggcttgtt
      241 gccctctctc taactctctc ccgcagggag agaggacagg gtggctgttg gggttaaggt
      301 ttatgcaaac taatacggct tgctgccctc tctctaactc tctcccacgg ggagagagga
      361 ctatgatgcc caccggcggc ttgctattag gaatatctga atcgtcattc ccacgaaagt
      421 gggaatctag aacgtcaaat ctcaagaaac cgttttatcc gataagtttc tgttccgaca
      481 ggtccggatt cccgcctgcg cgggaatgac gaatccatcc atacggaaac ctgcatcccg
      541 tcattcccac gaacctacat tccgtcattc ccacgaaagt gggaatctag ttttttgagt
      601 ttcagtcatt tccgataaat tgccttagca ttgaaggtcc ggattcccgc ctgcgcggga
      661 atgacgggat ttgaggtttc tgtttttaat tttctgtttt cacgggaatg acggtttaaa
      721 agttacccga aacctcaaaa aaaaaaagct gtgttttaat atagtgttga tagacgtact
      781 tggcctccat gtattcaatc gtggaaatct atatcttcgt cctcgccgaa atagtctatg
      841 cccgatatac aattttgata cacaaacttg gaaatatggg tatcgtcgcc ggagcgatag
      901 aatgcggaca gttatatata tacggttttt ttaggggagc gggcagatga agggcggatt
      961 ttgaattaac cccatgcaaa ttgacttttg cgggcggttt taccgcccat atatttacaa
     1021 aagccaaatt tctaaacata tatccttgat atatacacgg cgtaaacata tactggaaac
     1081 atctttaaat tttccgaaat tttaaacatg agcaactgga aacccgatat tccctataac
     1141 gatttaccac ccctgccgcc aaaacaggat attgaaagca aaaccatcct gaaacgttgt
     1201 atagccgccc gtgcatccct tgcccgttta aagcaggcgg cagaattgat accgaatcaa
     1261 gccatgctga ttaacaccct tcctgttatg gaagcccgtg caagttcgga aattgaaaac
     1321 atcgtaacca ccacggacaa gctgtttcaa tccctgcaaa tggatacgga acggcaagac
     1381 cctgccacga aagaagccct gcaataccgc accgccctgt ttgcaggcta tgaatcactg
     1441 gcgagccgcc ctttatgcac acaaaccgcc atcatggtct gcaacgccat caagcacccc
     1501 tacgaaacgg ccatccgcaa aacaggcggc acagccctaa aaggaggtaa cagcggaaat
     1561 gttgtctata ccccgcccga aggagaagaa accatacgcg gcaagctggc aaattgggag
     1621 cggtttattc acgaaagcgg cgatttagac ccgcttgtca tcatggcggc ggcacattac
     1681 caatttgaag ccatccaccc gtttacggac ggcaacgggc ggacgggacg catattgaac
     1741 agcctgctat tgattgaaaa agggcttttg gatttgccta ttttgtattt gagccgctac
     1801 atcatcgaaa acagggcgga ctattaccgc ctgcttttag gcgtaaccga acggcaggat
     1861 tgggaaagct ggataatcta catcttagac ggcgtagccg acaccgccga ttggacggta
     1921 tcgaaaatag atgcgatacg ctgcctgttc gagcagacac gtcaacacat acggacacac
     1981 gcacaaggaa tctacacgca cgaactggta aatcttctgt ttgagcagcc atatacacgc
     2041 attgccaacc tagaagcggc agggatagcc aaacggcaga cggcttctaa gtacctgaaa
     2101 gagctttcag gcataggtgt gctgcaagaa atcgccatcg gcagggacaa actattcatt
     2161 catccgcgcc taatggaact attgcgggga gagggcaaca gctttacttc attctaaccc
     2221 cctcttcatc cctacatgac taacacgaaa cagggatttt gacacccgaa ccggtacccc
     2281 ttgtattttc cccgcgaaaa gccggcatcc gcccgcgcat catgggagca acaaaacccc
     2341 tgcctaaaat tttgacttgt gcaaattagg ggtatatttg gggggtatat tgaaaaatgg
     2401 ctaaaataaa atgtttaata aacaaaatgt tgaaacttaa tttcgataga gcctctgcat
     2461 atcgtattga ggcgttaatg gaatttgaga aagctatttt taaataagaa aaggtaacta
     2521 ttaaatagct acctttctaa aattaaatat caacacatcg ttaaaacaca gccaaaaaaa
     2581 aaccgaaacc gaacggtccg gattcccgcc tgcgcgggaa tgacggattt caagattacg
     2641 gtattgtcgg ggatgatggg aaacggtggg aattgtgtaa aaaatgccgt ctgaagcctt
     2701 tcaggcggca tttgcggcat tcggacattt aaaaatcccg tcattcccgc gcaggaggga
     2761 atccgaatac atccgcacag aaacctgcat cccgtcattc ccgcgaaagc gggaatctag
     2821 aacgtaaaat ctaaagaaac cgttttatcc gacaagtttc cgcaccgaca ggtctagatt
     2881 cccactttcg cgggaatgac gggattttag gtctctgtaa tctcgggcag ctttccgtta
     2941 tcgttttaac ccaaagacac catttcaatc tgctccatcg tcctgcccag aaaacgctcg
     3001 ccgatggttc tgaatttcaa aggaatatcg ctgacgtaaa aacggtagtc gggattgttg
     3061 ttgccggtat cgagcaatcc ttcctgtgca aggacgcgtg cggtttcttc ggccgtcgta
     3121 atcgcggaat cgaccagcgc gacattgtgc gcctccctgc cgattaaggg cttgagcaag
     3181 ggaaagtgcg tgcagcccaa caccagcgta tcgatgccgt ctgcaagcaa tggtttgagg
     3241 tattcgcata cggtcaggcg ggtaacttcg tgttccaacc aaccttcctc caccaaaggg
     3301 acgagcagcg gcgcggcctg cgtgcggacg agcgtgtcgg ggttgtccct gtggatggcg
     3361 cgcgcataag cattgctgtt gactgtcgta ttggtggcga tgatgccgat tttattgttg
     3421 cgcgtcgttg ccagcgcggc tttggcgccg gcggaaatca cgtccaaaac gggcatattg
     3481 cccgtctttt gacggatttt ccgccccgca accgccgcaa tcgtgttgca cgcgataacc
     3541 atcgccttga catcgtgtcc caataaaaaa tcgacaatct gcatcgagaa attttcgatg
     3601 gtcgccttag atttcgtccc gtaaggcacg cgcgccgtgt cgccgaaata aatgatgttc
     3661 tccatcggca gccgttccat cagcgcgcgc acattggtca aaccgccgat tcccgagtca
     3721 aaaacgccga tgggccgctg cctgccgata ttttccatcc tttttccaat ccgtcctaaa
     3781 catacaatcc gcatattgta cacgcgccgc tttttcttga cagccatgcc gtctgaaagc
     3841 aggatgcgga tttggctgtt ataatgtacg gcaatgccgc cgccgggcgc acggttttcc
     3901 gaccgcctgc ccctccgatc cgccacataa aagttgtaaa gcccctatgt ctgatttttc
     3961 ccccgtttcc cgattccttg ccgacgaagc cgccacactc gatttgggcg cggcgtggtc
     4021 ttcccgttta aacgcaccgc tggtcattta cctcgaaggg gatttgggtg cgggcaaaac
     4081 cacgctgacg cgcggcatcc tgcgcggatt gggtcaccag ggcgcagtca aaagcccgac
     4141 ctacgccatc gtcgaatctt atccgctgga acgcttcgcc ctgcaccatt tcgacctcta
     4201 ccgcttctcg ttccccgaag aatgggaaga cgcggggctt gacgagctgt ttgccgcaaa
     4261 cagcgtctgc ctgatcgaat ggccgcaaca gggaggcgaa tttaccccgc ctgccgacat
     4321 caccgcaaca ttgacacacg gcggcggcgg cagaaaatgc ctgctgaccg cccataccga
     4381 acgaggacgc gaaagcctgc cgctatgacc aaactgacac gaagacaaat catccgccgc
     4441 accgccggca cactgttcgc cctgagcccc atcgcatccg ccgttgccaa aacggtacgc
     4501 gccccgcagt ttaccgccgc acggatatgg ccgtcgcaca cctacacccg cctgacgctg
     4561 gaaagcaccg ccgcgctcaa ataccagcac ttcgcgctcg acaacccggg caggttggtc
     4621 gtcgatatac aaaacgcaaa catcaatacc gtattgcacg ggctttccca aaaagttatg
     4681 gcggacgacc cctttatccg cagcatacgc gcgggtcaga acacgccgac caccgtccgc
     4741 ctcgtcatcg acctgaaaca gcccacccac gcacaagtct tcgcgcttcc gcccgtcggc
     4801 ggctttaagg accgcctcgt cgtcgacctc tatccgcacg ggatggatgc cgacgatccg
     4861 atgatggcgc tgctcaacgg cagcctgaac aaaaccctgc gcggctctcc ggaagccgac
     4921 cccgcccaaa acaccacgcc ccgacccggg cggggcaaaa acgggcgcag acccgtcatc
     4981 atgctcgatc cgggacacgg cggtgaagac cccggcgccg tcagcccggg cggtttgcag
     5041 gaaaaacacg tcgtcctctc catcgcccgc gaaaccaaaa aacaattgga agccttgggt
     5101 tacaacgtct ttatgacgcg caacgaagac gtgttcatcc cattgggcgt gcgtgtcgcc
     5161 aaagggcgcg cacggcgggc ggacgtattc gtctccatcc acgccgacgc tttcaccagc
     5221 ccctccgcgc gcggaacggg ggtttatatg ctgaacacca aaggcgcgac cagctctgcc
     5281 gccaaattct tggaacaaac ccaaaataat gccgacgcgg tcggcggcgt accgaccagc
     5341 ggcaaccgca acgtcgatac cgccctgctc gacatgacgc aaaccgccac actgcgcgac
     5401 agccgcaaac tcggcaaact ggtgcttgaa gaattgggca ggctcaacca tctgcacaaa
     5461 ggcagggtgg acgaagccaa tttcgccgtt ttgcgcgcac ccgatatgcc gtctatcctg
     5521 gtcgaaaccg ccttcctgtc caatcctgcc gaagagaagc tgctgggcag cgaatccttc
     5581 cgtcggcagt gcgcccaatc cattgcctcg ggtgtccaac gctacatcaa tacatccgta
     5641 ttgaagcggg gttgattgaa aaggcggtgt ttcaaaatat agttgtcgga tgaaggcagg
     5701 cataaaccta cttccgcccc aaaccgcgtt tttatgatga aaggtatgga taaattgaga
     5761 taccaacggg attttttaaa tatccgcccc atttttacag ccggcgaaca agaatacctg
     5821 accgaattgt ccgatcggct tcctttatct gttttaacgg actccgtgcg taatattgaa
     5881 gaaatcggta tcgattttgt gtacagtcct gccaaattgg aaggaaacac ttataatcaa
     5941 tacgatacgc aggcattgct taaactcggg caaaccgcag gcgggaagtt atattcggat
     6001 gccgtaatgt taatcaacct gagggaaagt taccggcacc tgctgtccgg tttggacagc
     6061 cccaagccgt ttgactggtt ggatttctta aaaaccaccc acagcctgat ttcggaaaac
     6121 ttactggaaa aaggctccgg cggcgtagtc cgccgcgaca gcgttactat aagcgggaca
     6181 gactacaccc ctttatccaa cccgcaaagt ttggatacgg aattgaaatg gttgcttcaa
     6241 gaagccccca aaatagaaaa tcctttcgac cgcgccgttt atctccataa taatttggcg
     6301 tatctccgat attttaagga ctgcaataaa cgtactgcca gaaactgtat gaccttgtcg
     6361 ctgatgcgct ccggtttttt tccttgcgta ttctcccccg acagttatcc cgcctatgcc
     6421 gaggctgtcg tagcctatta cgaaaccggc gattacggtt tattcaagaa atattttatt
     6481 tcggcatacg aaaataccgt aaataagtac ggtccccagc ctgatgtgga tattttccga
     6541 aatttctcca tttaaaaagc cgtctgaaca tttttcagac ggcatttttg tacactttcc
     6601 tcaacggttc gatccctgca ccatatcgat caggaacagg cggcagtcga tgttgccgtt
     6661 ataaagcggg attttccgct tgggcagcag gcgcatgaat ttgggcattt ccctgtcgcc
     6721 ggtaaacatg ccgacccgcc agccggcata gtgttttttc aaccacgtac ccaactgcgg
     6781 ataaagtgcc tgcaaagcgc ggacttcctc aaggcgcacg ccgtagggcg gattggacac
     6841 catgatgccg ttttcgccgt tcggacggac ggactgcgcg ccggcaacgc tgaaggaaac
     6901 gatgtcgtcc gctccggcat tgtgtgcgtt gtccaatgcc gtctgaacga tgcggcgacc
     6961 gttgtcgctg cctgcaatcg gggcgcggac ggggcgggtt tgcgcttcgg cgcggcggcg
     7021 caaatccgcc cacagcgttt tatcgaaatt ttgcagtttt tcaaaaccga agcggcgcat
     7081 catacccggc gcgcggccgg cggcaatcca agcggcttcg atagcaatcg tgccgctgcc
     7141 gcaaaacggg tcttgaaacg gctgcgtgcc gtcgtagcct gccgagagca gcagcccggc
     7201 ggcaaggttt tcgcgcagcg gggcttcgcc ggtatccagg cggtagccgc gtttgaacag
     7261 ggcttcgccc gaagtgtcga tgaagatttc gacattgcgt tcgtccaaaa aggcgtggat
     7321 gcggacatcg ggcgcggctt tgtccacgct cggacgtgcg tcgtaaatgt cgcggaaagc
     7381 gtcgcagacg gcatctttga cggtcaggcc ggcaaattgg aggcttttga cgttggcgcg
     7441 ttttgcctcg attttgactt tgaacgtctg ctgcaaggta aaccagttga accagttgat
     7501 gtttttggca agtttgtaga tgtcgcgctc ggtgcggtat gtgcctttgg tcaggcgcag
     7561 caggatgcgg ccggcggtac gcgagtgcag gttggcggcg taggcctgtt ccaatccgcc
     7621 ccggcaggaa acgccgccgt caaacgcttg gacatcggta cagccgaggc ttccgagttc
     7681 ttgagataaa acggtttcca agccgcgcgg gcaggtggcg aaaagtgtat ataaagtatt
     7741 catagcgtgt cctttccgga cggatgttcc ggcaatggcg gattgaaagg tttattcggc
     7801 ggtaaatgcc gtctgaaatg tccggctgcc gattgtaatg tccgccgtaa aatcgcgcct
     7861 gccttcgaca cagacgggca ggcggatgcg tgctgcctgc caagtccccg acggttgccg
     7921 ctcgaacata tagcggttga aacccatatc catatttttc atactgaagc tgatgctgac
     7981 ctgttccgtg ccggcgggcg cgtgttcgat ataaatatca aacggttttt tggttgaaac
     8041 ggcggcggcg cggacacggc ttccgtccgg cagcgtgcaa ccctcggtca aatcgcattg
     8101 cgccgccacg gcttgcggct gctgcggctg ctgcgcctgc caccattgca acaaaacgag
     8161 cttgaaggcg gcaaaggcag tcagcagcag tgcggcaagc agtaatttgc ggtttttatt
     8221 catgattctt ccctattgtt tcatccctta ccaccgcgtg cgcccccaat ccgagccata
     8281 gttttccata acgtgcaacc gtctgtccgt ttgctgcaag tacgagcggc acggatacgc
     8341 cctgccgcag ggcttctgcc gcctcttgtg cctgaacatc atcgcacacg cgccacacgg
     8401 ccacatccgc gcccttttcc tgcgcctgcc gccattgttc gcgggtctgc accatcatcc
     8461 acacgctgcc ggcgtgttcg gacctgtcct gccattgtgc cgcctccatc aaaacgttcg
     8521 caccgcttcc ccctgccgaa cccaaaggca ggacgcggta cgcgcccata ctgttttcgc
     8581 cgtacaaacc tgttttcagg ctgccgtaca aacggcgcgg gacggacagc gaacgcaaaa
     8641 gcgcaccgtt ggcgggcagc atgggggcaa cggtaaaatc acccgccttc tgccaagacc
     8701 attcctgccc ttcgcgcgat tgcggtccgc cctcccattg atcggggttt acccataaga
     8761 atttcaggca gacgcgggcg tgttcgtagg aatggatttt ggtcagccaa ggcgtggcgg
     8821 cgaggatgcg gatgccgagt tcttctccaa actcgcgttg cagggcttgg aagtcggttt
     8881 cgcccgcctc gaccttgccg ccggcaaatt cccaatatcc ggcatagggc ttgccttcgg
     8941 ggcgcgagcc gagcaggtaa ttgccgtctg aatcgagcag gatgccggca acgacgcgga
     9001 taagggggcg ggtatcttgg atcatggcgg gtatgcggtt ggtcggtcgg aatgcaaatg
     9061 agggaagggc aatgccgtca acgggtttgc cgaatttcag acggcattat ttttcggcaa
     9121 cggcaaacgc caacacggta tcgccttcgt ccgccatact caggctgacg cggctgatgc
     9181 cttgttcttc cagccattcg gacagggcgg ggccgtagaa aaattcgggc ttgcccaatg
     9241 cgtcgtgccc gatgccgatg ttgcggaagg aaaccgcgcc tcgtatgccc gtgccgacgg
     9301 ctttggcgaa ggcttctttg gcggcaaagc gtttggcaag gtagttgacg ggtttgcccg
     9361 cctgcggaaa ttcaagcagc tcttcgggag tgagtatgcg ccctgcaaac gcctgtccga
     9421 attttttgtt taagcggatg atgcgcttga gggaaacgat gtctgtaccg atgccgtaaa
     9481 tcatatttgc gctccttttt cgtgaaatgc cgtctgaaac ggtttgcctg cccttacggc
     9541 agcagccttg ccctgaacat cgcctccttc atctggcgca cggcctcggg caatccgagg
     9601 aaaagtgcct gcgaaatcag cgaatgcccg atgttcagct cgcggatggc gaggatttgg
     9661 gcgacggggg taacgttgtg tatggtcagt ccgtgtccgg cgttgacgac caagcccaaa
     9721 tcgccggcga aatgcgcgcc gttttgcaga cgctcgaact gcctgatttg ttcggcgtgg
     9781 ctgtgtgcgt cggcatatgc gccggtgtgc agctcgacaa cgggcgcgcc gacatcatag
     9841 gcggcttgga tttgcctgtc gtcggcatcg ataaacaagg acacgcgtat gcccgcgtcg
     9901 gtcaggattt tggcgaattc ggcaattttt tcttgttgcg ccaatacatc caagccgcct
     9961 tcggtcgtga tttcctgccg tttttcaggc acgatgcaca cgtcttccgg catcacttta
    10021 agcgcgtttt cgagcatttc ttccgtcaac gccatttcaa ggttcaggcg cgtgcggatg
    10081 gcgtttttga cggcaaatac atccgcgtct ttgatgtggc ggcggtcttc gcgcaggtgc
    10141 atggtaatca ggtctgcacc gtgcgtttcg gcaaccagtg ccgcctccac ggggctggga
    10201 taagtcgtgc cgcgcgcgtt gcggacggtg gcgatgtggt cgatgttgac acccagaagc
    10261 ataatgtttc cctttctctt cgttttcctg tttgacggca tggtgcgggc gcagctacgc
    10321 ggtattgcgg tcgaactgcc ttatctgttc caacacctgc cgcgatttca agccttcggg
    10381 aagcagcgtg ccgattagca gccgtgtgat tttcaatgcc tgttgcaggc tttcggcggt
    10441 gcggaaactg ccttcgcgca aatcgatcag gctctgccct tcgacaattg cgccggcttc
    10501 gtgcgaaagc gcgcctgtgt cccggcatac gggcatgacc gcctcttcgg gcatcagccg
    10561 gtaggttttg tccgccaaaa tatcgccgcc cgtgccgtcg gcgtgcaaat cgggggcaac
    10621 gcccaaggca ttgagcagct tccattcgaa gcggcgcaaa tcggcaatat ggttggcttc
    10681 acggcacact gcctccatca cttttgccaa cgcgtcgtac agttcggaca tcgggtcttc
    10741 gcgcgcggtc agtttcaaca ccagctcgtt cacatacaga ccgctgaaca acgccctgcc
    10801 ctgcggctgc cgccatccgc ccatccattc ggcgcggtgc agggttttga gttcctgact
    10861 gccgtaccac gacacgctgg cagggacgaa cggaaccaat acgccgcgca gctcgctctg
    10921 ccgtttgcgc gcgctgcgcg ccagcaaagc cacacgcccg taacggcggc tgaatgcttc
    10981 aacccgcagg ctgctttcgc gccaaggcga agatgccagc ataaaaacgg gttcgtggtt
    11041 gactcggtgt tcggacataa tgcctgaaat gccgtctgaa cgctgccgcg actacaaaac
    11101 ggcagtcgga tacgaaccga tgactttgac aaacgaagcg cgttcgccca agcgttccaa
    11161 tgccgtctga atctgcgcgt cccggcggtg tccttcgatg tcgatgaaga acaggtattc
    11221 ccacaaaacg gatttgctcg gacggctctc aaacttggtc atggaaatac ccgattcggt
    11281 cagcggttgc agcagcgagg caaccgcgcc ggcgcggttg ggcgcggaaa cggccagcga
    11341 ggttttgtcg ctgccgcttg caccggtttc gtgatgtccc atgaccagaa agcgcgtggt
    11401 gttgttcggt tcgtcttcga tgcactcggc aaccatatcg agtccgtaaa tttccgccgc
    11461 cgtgcgtccg gcgatggcgg caaccgtacc gtcgtccgat tcggcaacca gccttgcggc
    11521 ttcggcattg ctggacacgg caatccgttc ggcgttgggc agacgcctgc ccaaccagtc
    11581 gttgcactgc gccaacgcct gcgcgtgcga aaatactttg gcgataccct cggtgctgcc
    11641 gttgtttttg cgcaacaggt tgtggcggat gcgcaaaacg acttcgccgc acgcctgcaa
    11701 cgcggtaacg gcaagcaaat ccaatgtgcg cccgaccgag ccttcggtcg agttttccac
    11761 gggcgcgacc agataatccg cctgacgggt ttcaacctgt ttgaagcagt cgtctatggt
    11821 cggacacgcc attgtatgcg cggcatgacc gaaatgctta atcgccgcct gctgggtaaa
    11881 cgtgccctgc ggccccagat agacaatggt cagcggacgt tcgacggcga ggcactcgct
    11941 catcacttcc cgaaacaggc gtgctaccga ttcgtcgggc agcgggcctt tgttcaaatc
    12001 ctgaatgcgg cgcaacacgg caacctcgcg ttcggggcgg tacaccgcgc ccgtgccttt
    12061 cagctcgccg atggcgtggg catgttgcgc gcgttcgttg agcaggcgca ggatttcggc
    12121 atcgatggtg tcgatggcgt tgcggtgcgg gatgaggagt tcgtcgatag tttgggacat
    12181 aatttcgcct gaacggtaat ttgccgtgca ttctaacatc aaatgccgtc tgaagcggtt
    12241 tcagacggca ttttccgtta aaggtattcg ctttgcccgt tttctttcca cgcacgatgc
    12301 gagcagagcc acaaaagcgc aatgccgcaa gaggcggacg cggtcatggt tgccgccatc
    12361 acggttgccg aaccgtcgtg caggacggtt gccgccatgc ccacgcccgc gccgattaag
    12421 gaccggaata cacccgacac ggcgttcgcg ctgccgccct cttctttgaa ataagacata
    12481 aagcatgcct gcgtgtccgc accgaccagg ccctgcgtac cgacggaaaa catcacgcac
    12541 gcaaccggca gccaaaacgg gggcaacccg aaaaacagca cggcggcgag ttgggacggg
    12601 ttggcggcaa attggacgac aatcccccgc agcaggatgc tttgcggatg cgcgccggtt
    12661 ttaagccgcc acgcggtaac gcggctgaaa aacatcatcg tgatgatgtt gagtgcaaac
    12721 acccatgcgt accggtgcgg cgtaacgtgg tagagctgcc ggtacacgaa ggaagattcg
    12781 gtcagaaagg cgaacatcga accgaagctg aatgcctgaa aaaacagata acccatcgcg
    12841 gcacgggttt tcaatacgcg cttgaaccgc cccgccacca gcccgaacac atccctgccg
    12901 attttgccgc cgacggcggg attgggcagg aaatactgta ccaaaccggg cagcaccggc
    12961 gaatacgccg ccaagaaaac gaaaatcgcc cgccatccgc ccaatccctg caacaatgcg
    13021 ccgaccatgg gtgcggccag cggcacaacc atcagaatga tgccgataag ggcaaacatc
    13081 tgcgcggctt tgcgtccgga ataataatcg cgcaccatcg caccgacgat gactacagcc
    13141 atgcctgcgc cgaacgcctg taccgcacgc aggttaagga gctgttcggt actcgaagca
    13201 aatacgatgg cggcaacggc aaggcaatat acaatcaaac cggtcagggc gacgggtttg
    13261 cgccctttga tgtcggacac cgcgccgccg gccacttgcc cgaacgccgt gccgaacata
    13321 aacaaactca gactctattc gatacggtgg atatccgcgt tcagcggctg cgccatttcg
    13381 ggaatcgcgg gcaggtaggc atcggtggaa aacggcatca gcgcgaccag taccgccatc
    13441 aaaaccgcca tcagttttcc gctcatcgga ggaaggggcg gagacatggt ttttccgttc
    13501 aaatcaaata aagaaaccgt cgcaaacgac ggtcggtacg ggcgcaagtc taacatatcg
    13561 gacggcgggg caaaaccgaa ggatatgccg cccgaaaccg cttcagacgg cattgccgtt
    13621 ttgcgcgtcc ctgcgcgaca gccggccggc ggcgaaagtg gcggcgatga cgacaaacac
    13681 gcccaaggcg gacacgggcg ataaatgttc gcccaaaatc aaaaccgcca acagcacgcc
    13741 gacgacgggt tcgagcgaaa tcaacagtcc cgacgcgttg gcaggaacac ggctcatccc
    13801 cttgttccac agccaatagg cgtaccagcc gcaccccaaa cccaaataca acagcgacaa
    13861 taccatcccg acgctccagt ccacggtata actttgcgcc aaagcaagcg aaaacggcag
    13921 gcacatcaac gatgcggcgg caatggaaac agatgtgaat gccggtgcgc cgatgcgggc
    13981 aatcagcctt tgcgtcggac gcatagcggc acaaaagccc gcgcccgcca acaacaccag
    14041 caggcagccg aaccagccga cttcgccgcc ctcttccgca ccgcccgcca tcagcagcgc
    14101 gacaccggca aatgccgccg cgccgcatat ccagtggtag gcacgcgctt tgtcgttgaa
    14161 gaaaaagtgt ccgacaaaca ccatcagcag cggctcgagt ccgacaatga ccgatgcgct
    14221 ggcggcggaa gtgtatttca acccgacaaa ctgaagcagc agggtcagca catagttgac
    14281 gaacgacaca atcagcaacg gcttccattc ctcacgcgga atcttgccga catgacggcg
    14341 gcaggcgggc agtgcaggca gcgcggcaat cagcaggcgc acgccgacca tcaatgcggg
    14401 atcgatgccg ccatagacat atttggcggc aataaacgag ctgccccaga taatcagggc
    14461 aaggatttgg taaaacatag tgagtggtaa agtcagatgg tcatacaaaa gtatagcgga
    14521 ttaacaaaaa tcaggacaag aaggaatgcc cgaaccgtca ttcccgccac ttttcgtcat
    14581 tcccgcgaaa gcgggaatct ggaatctcgg actttcagat aatctttgaa tattgccgtt
    14641 gttccaaggt ccggactccc gcctgcgcgg gaatgacgaa gccatccttt tggccgaagg
    14701 tcaaaaatca gccgtcaccg agtattacct gaatcacggc atatggccgg aaaacaacga
    14761 ctctgccggc gtggcatccg cttcaaaaat cataggcaaa tatgttaaaa gcgttacggt
    14821 cacaaacggc gtcgttaccg cccaaatgaa accaagcggc gtaaacaatg aaatcaaaga
    14881 caaaaaactc tccctgtggg ccaagcgtca agacggttcg gtaaaatggt tctgcggaca
    14941 gccggttaag cgcgacgccg gcgccaaaac cggcgccgac gccgtcaccg ccgacaaaga
    15001 caaagaaatc gaaaccaagc acctgccgtc aacctgccgt gatacgtcat ctgccgaata
    15061 aggcaaatta ggccttaaat ttcaaataaa tcaagcggta agtgatttcc cacggccgcc
    15121 cggatcaacc cgggcggctt gtcttttaag ggtttgcaag gcgggcgggg tcgtccgttc
    15181 cggtggaaat aatatatcga ttgcgcttca aggccctgca tgtgcctcat tgccacccgt
    15241 ttaaacacgg tttttatctg acaggcgcgc aatccgcccc cccatttgcc gaacaagcgg
    15301 tccggactcc cgcccgcgcg ggaatgacgg ctgcagatgc ccgacggtct ttatagcgga
    15361 ttaacaaaaa tcaggacaag gcggcgggcc gcaggcagta caaatggtac ggaaccgatc
    15421 cgcccggtgc ttcatcacct tggggaaccg ttccctttgg gccggggcgg ggcaacgacg
    15481 taccggtttt tgttcatccg ccatattgtg ttgaaacacc gcccggaacc cgatataatc
    15541 cgcccttcaa catcagtgaa aatctttttt ttaaccggtc aaaccgaata aggagccgaa
    15601 aatgaatcca gcccgcaaaa aaccttctct tctcttctct tctcttctct tctcttctct
    15661 tctcttctct tctcttctct tccgcagcgc aggcggcaag tgaagacggc ggccgcggcc
    15721 cgtatgtgca ggcggattta gcctacgcct acgaacacat tacccacgat tatccggaac
    15781 caaccggtac aaaaaaagac aaaataagca cggtaagcga ttatttcaga aacatccgca
    15841 cccgctccgt ccacccccgg gtgtcggtcg gctacgactt cggcggctgg cgcatcgccg
    15901 cggattatgc ccgttacaga aaatggaaca acagtaaata ttccgtcaac acaaaaaatt
    15961 tgcggacaca tgaaagcaat ggcaacaggc aagatctgaa gacgg
//
LOCUS       DAWTHD010000054        16005 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWTHD010000054 DAWTHD010000000
VERSION     DAWTHD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.054"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(297..719)
                     /locus_tag="WM704_001256"
     CDS             complement(297..719)
                     /locus_tag="WM704_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFA9340675.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            complement(837..1508)
                     /locus_tag="WM704_001257"
     CDS             complement(837..1508)
                     /locus_tag="WM704_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFA9340676.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1524..2150)
                     /locus_tag="WM704_001258"
     CDS             complement(1524..2150)
                     /locus_tag="WM704_001258"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFA9340677.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2257..3075)
                     /locus_tag="WM704_001259"
     CDS             complement(2257..3075)
                     /locus_tag="WM704_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9340678.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGK
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            complement(3335..3775)
                     /locus_tag="WM704_001260"
     CDS             complement(3335..3775)
                     /locus_tag="WM704_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFA9340679.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3838..5244)
                     /locus_tag="WM704_001261"
     CDS             complement(3838..5244)
                     /locus_tag="WM704_001261"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFA9340680.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            complement(5247..5429)
                     /locus_tag="WM704_001262"
     CDS             complement(5247..5429)
                     /locus_tag="WM704_001262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340681.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            5418..6533
                     /gene="lptF"
                     /locus_tag="WM704_001263"
     CDS             5418..6533
                     /gene="lptF"
                     /locus_tag="WM704_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFA9340682.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6530..7600
                     /gene="lptG"
                     /locus_tag="WM704_001264"
     CDS             6530..7600
                     /gene="lptG"
                     /locus_tag="WM704_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFA9340683.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7914..10499
                     /gene="acnB"
                     /locus_tag="WM704_001265"
     CDS             7914..10499
                     /gene="acnB"
                     /locus_tag="WM704_001265"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFA9340684.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPKLLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10641..11636
                     /locus_tag="WM704_001266"
     CDS             10641..11636
                     /locus_tag="WM704_001266"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFA9340685.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11755..12768)
                     /gene="ilvC"
                     /locus_tag="WM704_001267"
     CDS             complement(11755..12768)
                     /gene="ilvC"
                     /locus_tag="WM704_001267"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFA9340686.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12848..13141)
                     /locus_tag="WM704_001268"
     CDS             complement(12848..13141)
                     /locus_tag="WM704_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFA9340687.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            complement(13208..13699)
                     /gene="ilvN"
                     /locus_tag="WM704_001269"
     CDS             complement(13208..13699)
                     /gene="ilvN"
                     /locus_tag="WM704_001269"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFA9340688.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13710..15437)
                     /gene="ilvB"
                     /locus_tag="WM704_001270"
     CDS             complement(13710..15437)
                     /gene="ilvB"
                     /locus_tag="WM704_001270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFA9340689.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
BASE COUNT         3730 a         4371 c         4079 g         3825 t
ORIGIN      
        1 tatggcttaa aataacggga ttcccgcctt ccgcccgccc gcaaaatcag gcgggcatca
       61 ttttaaaacg cggcatttaa aatttgaccg aaaattgttg acaatccgga atcaagcctg
      121 cacaataccc cgacaagtcc aagtattata aaggctgaat aaagaggaaa cagcaggcag
      181 atatattcgg gaggtgcagt ccgaatatat ctgctttttt atgcgcctcc ggattgcctg
      241 ccgcaccttt ccccttcaga cggcatcagc cgtttcccca taatgccgcc cgatgcctat
      301 ttctccgccc cggcaatttc aaaactgtgg gtaatctttg ccgctttgcc caacataatc
      361 gaagccgaac agtatttttc ggcagacatc tgaacggcgc gctcaatggc cgattctttc
      421 aaatcatgcc cgattacctt gaaatggatg tggatttcgg taaacacgcg cggcgcatcg
      481 tccgcccgtt tcgccgtaac cgtcgcacgg cagtcagtca ctttctgacg ctgtttttcg
      541 gcaatcatca ccacatcgat gctcgaacag cccgccaccc ccaacagcag catttccaaa
      601 gggctgggcc cgcgcttggc cgcgccttct gccgccgatc cctccataac gacgctgtgc
      661 ccgccttccg ccgtgccgac aaaacacatt ccgtctatcc attttgatgt aacctgcatg
      721 gtgtcattcc cgaaaatagc gttaaaaccg ctttgcatat ggcgttattg taaacaattt
      781 caagcggctt atacagaaat atggacaaaa cggcaaaaaa ccgcctgaaa accgatttac
      841 ggtttggcgg cctgtccgtt gatctgcacc gatttgagct tcagcgtata ggttttgccg
      901 tcgtcggtat agccgatttg tgccggaata ttgttcaggg acggtgcgaa gaaatacgtt
      961 accgtatcgt cgccgcgccg cacccgatat ttgacgactt cggtttccac gccgcctatg
     1021 ctgtattttc ccgtacccgc cttattcagg ccgccgacgg aataaagttt tttgccgttg
     1081 gtgattttca gacccggggg gagtttcgcg tcatttgccg ccaactgcca ggcaagcgtg
     1141 aacaaatcca tagccttggg gctttgctcg gttttgctct cgcccgcttt gccgtaggtt
     1201 acgctgccgt cggcgaattt ggcttccgca tacagtttgc ccctgcgtat gtctttatag
     1261 taggcagggt gcagggtatt gccgacaacc gtaccgccgg actcgaaacg gatattgtat
     1321 agcggcactt taatcgtcga aacgattttg taagcattgc cgctgcgttc aaatgtcatc
     1381 gtggcgggaa tgccgtagct gccggaatag tgcagcacgg cggattgggg tagccttgcc
     1441 gcatacgcgc acggcagggc ggcggacaaa atggcggcgg aaaatatatt tttaaaagtc
     1501 ttcatcattt gctcccgccc ggtttacgcc gtcaggaaac gggcggcatc ggcgttttcc
     1561 gaatttctga cacggtttcc ctcgataatc aggcggccgg cggcaaaatc ggcaacggct
     1621 ttcggataga gtttgtgttc gacagccaaa acccgggcgg caacatcgtc tgccgtatcg
     1681 ccgtcaagta tcggcacaat cccttgcgat acaatctggc cgcaatccag ctcggccgta
     1741 acgaaatgaa tcgtacagcc ggcaacgcgg caacctgctt ccaaagcgcg ttcgtgcgta
     1801 tgaagcccgg tgaacgaggg caggatggac ggatggatgt tcatcagcct gccttcgtaa
     1861 tgggcgcaga actcaggggt cagaatccgc ataaaacctg ccaaaaccac caaatcgggt
     1921 tgatatgcgt cgattttttc catcatggcc gtatcgaagg caagtcgtga ttcaaagttt
     1981 ttatgattca ggctgtcggt cgggatgccg cgttcggccg cccattgcaa accggcagcc
     2041 gtttcgctgt tgctcaacac ggcggcaatg cggacgttgg gaatggcggc attgacgatt
     2101 gcctgcatat tgctgccgcg tccggaaatc aggatgacga tgtttttcat aatggtgcgc
     2161 ttttgaaagg gatgccgtct gaaccgctgt ttggtggctt cagacggcat ttgccgtaaa
     2221 aatgcccgaa aacctgtttc gggcatggat tcggacttaa tttacttttt tgatgtcgac
     2281 ttgatccggc tgcttggcgg gcgcgttttc gggtgcgccg attttgacca gtttcacgtc
     2341 aaataccaaa gtggcgttcg gaccgatttt ttcgcccgca ccctgttcgc ggtaggcaag
     2401 gttggacggg atgtagaacg tggcttcgcc gccttctttc agaagccgta cgccttcggt
     2461 ccaacccgga atcacttggc tcaaagggaa ggtggccggg ccgccgttgg ctttgctgct
     2521 gtcgaatacg gtaccgtcaa tcaggcggcc ttcgtattcc acggtaacga tgtcgtcttt
     2581 tgtcggctgt ttgcctttac cctgtttggt gattttgtac tgcagaccgg aagcagtggt
     2641 cttcacgccg tctttggcgg cattttcctt caggaaggct tcgccttttt ctttgttggc
     2701 cttcgcatcc gccttgtgtt tttctacggc tttagcctgc tgctcctgca ggaatttcat
     2761 catcacttcc tgggcctgct cttcggtcat tttgatttct ttgccgtcat acactgcctg
     2821 catggcatcg gtaaagactt tcaaatcgat ttccgcgccc tgttccttca tttgtttcag
     2881 ggagcgtccg atgtccacgc ccatcgcata gcttgcctgc tgcatcgtgc cgccgattga
     2941 agaggtgtcg ccctgcgcgg cagaagcggc ggcaggttcg gatgcagatg cgggggcggc
     3001 ttcttttttg ccgcaggcgg aaagcgccaa agcggcggaa agggtcagtg cgctgatttt
     3061 gaaaatggtg ttcatgatgg atcttcgctg tcgataaagt cggaaaaacg ggattatagc
     3121 cgagtttgaa tgtttcaaca cacaggatgg cacataaagc gtcaatctgt gtgttgccct
     3181 gttttggaag ggattgaacc ttccaaaata aggtttgatt ctaccgcccc gaaggacaga
     3241 tgtccaagtg gcggggttca accgataagg aaattttaat caaatagaat caagcctgtt
     3301 taaattttgt aaatgcggca tttcagacgg catttcatgc cttgccctcc atgccgtgat
     3361 gttcgatggc aaaaccgctt cggcggtagg cggtaaagcg ttcgcgcgcg tcggcgagat
     3421 cttccaggct gttgccgacg atttccaaaa cgcgcgcggg caagaccgaa gcggtgttcc
     3481 aaaaaccgtc ggacaggttc aaaacggcca tgccttcggg aattcggggc agattgccgc
     3541 cgcaggcaag caggacggat gtgtcagacg gcatggcttc ttccgtttcc caaatttcgt
     3601 gcggaataaa actctcggcc tcgtattgcc aaagcatttt gtccaattcc tgaagccgcc
     3661 cgaacgagtc ggaccacacc agtatcctgc cgccgtctcg gatagcgcgc gcaatcaacc
     3721 gacaggtaaa aagggggatt tggtcaacat gggtataaaa ggtaactttc ggcatattgt
     3781 ttgagcattt gacaggatga tgccgtctga aaagcttcag acggcattgt ggcaaaatta
     3841 aagattccgc agatagttca tcagcagcgg tacggggcgg ccggtcgcac ctttttccgc
     3901 tccggatttc cacgccgtac ccgcgatgtc aaggtgcgcc cacgggtagc cttcggtgaa
     3961 ataggacagg aatgttgcgg cggtaatcgt gcccgcgccg ggcgtgccga tattggggat
     4021 gtcggcaaag ttggatttga gctggtcttt gtaggtttca aagagcggca gttgccacgc
     4081 tttgtcgtcc acgttgtagg aagcggcaag caggctgtcg atcaaatcct gattattgcc
     4141 catcacgccg ctgacatcgt gccccaaggc gacgatgcac gcgccggtca gggtggcgac
     4201 atcgatgacg gctttgggtt tgaactgctc ggcgtaagtg agcgcgtcgc acaaaatcag
     4261 acggccttcg gcatcggtgt tcaacacttc aatcgtcagc cctttcatac ttttcacaac
     4321 atcgcccggt ttgtttgccg cgccggaagg catgttttca caagtggcga cgatggcaat
     4381 cagattgatc ggcagttgca gtttgacggc ggcgcagaag gtgctgatga cggttgccgc
     4441 gccgcacatg tcaaacttca tttcgtccat gttcaggccg ggtttgaggg aaatgccgcc
     4501 ggtgtcgaag gtaatgcctt tgccgaccaa taccacaggc gcggcttctt tgtcggccgc
     4561 accgaaatag ctcagttcaa ccaaatatgg gtcttcgacg ctgcctttgg caaccgacca
     4621 aaacgaaccc atgttttctt tgatgtagtc tttttcgatg attttggcgt gcgcgcccag
     4681 tttctcggct tcggctttgg cggtacgcgc gaggaactca ggcgtgcatt cgttcggtgc
     4741 ggcattgcct aagtcgcggc agaggctttg tccgtaaact tgtgcttcgg caacgcgcag
     4801 ggcttctttg acggcggctt cgtgtgcgct gtggaacacg gcttgcgaaa atttggcggg
     4861 cttggcttct tttttgtagc ggtcgaaacg atatgcagcg ttgccgaacg cgatggcaaa
     4921 agcttcggca acggctgctg cttgcgcctc atcaaaggcg tgaacgtcca cattgaccgt
     4981 ttcttgattt tgcgcccatt tggcggcttc ggcggcggct ttgttcagcg cggcgcggtc
     5041 ggttttttcg aggcggacga cggcgactgc ctgcaaaccg ttgtccgtcg ggattttggt
     5101 gtcggaaaaa ttctgaccct cttccaaagc agagaagagc gcatgtgcgg cggtgttgtg
     5161 ctcgggtgct ttgtcggcac agacaaataa ctgcgcgcct gcctgctgtt cctgcaagat
     5221 ttcggttttt gtgctaaatt ccacgtttat tctcctgatt gagacggttg tcggtagttt
     5281 tcagacggcc tttcgctcaa aagaccgtct gaagacggct ggcacgattg taccccattt
     5341 tttgaagcac cgtctgaaac cttgcgcgga caatccgcct gcgccgaacc gcttaccgcc
     5401 cccctgaccg cgattttatg atttatcaaa gaaacctcat caaagaactc tcttttaccg
     5461 ccgtcggcat tttcgtcgtc ctcttggcgg tgttggtgtc cacgcaggcg atcaacctgc
     5521 ttggccgcgc agctgacggg cgtgtcgcca tcgatgccgt gttggcctta gtcggcttct
     5581 gggtcatcgg tatgaccccg cttttgctgg tgttgaccgc attcatcagc acgctgaccg
     5641 tattgacccg ctactggcgc gacagcgaaa tgtcggtctg gctatcctgc ggattggcgt
     5701 tgaaacagtg gatacgcccc gtcatgcagt ttgccgtgcc gtttgccatc ctgattgccg
     5761 tcatgcagct ttgggtgata ccgtgggcgg aactgcgcag ccgcgaatat gccgaaattt
     5821 tgaagcagaa gcaggaattg tctttggtgg aagccggcga gttcaataac ttgggcaagc
     5881 gcaacggcag ggtttatttc gtcgaaacct ttgacaccga atccggcatc atgaaaaacc
     5941 tgttcctgcg cgaacaggac aaaaacggcg gcgacaacat catcttcgcc aaagaaggta
     6001 acttctcgct gaacgacaac aaacgcacgc tcgaattgcg ccacggctac cgttacagcg
     6061 gcacgcccgg acgcgccgac tacaatcagg tttccttcca aaaactcaac ctgattatca
     6121 gcaccacgcc caaacttatc gaccccgttt cccaccgccg caccatttcg accgcccaac
     6181 tgattggcag cagcaatccg caacatcagg cagaattgat gtggcgcatc tcgctgaccg
     6241 tcagcgtcct cctgctctgc ctactcgccg tgccgctttc ctatttcaac ccgcgcagcg
     6301 gacataccta caatatcttg attgccatcg gtttgttttt aatttaccaa aacgggctga
     6361 ccctgctttt tgaagccgtg gaagacggca aaatccattt ttggctcgga ctgctgccta
     6421 tgcacatcat catgttcgtc atcgcaatcg tacttctgcg cgtccgcagt atgcccagcc
     6481 agcccttctg gcaggcggtt ggcaaaagtc tgacattgaa aggcggaaaa tgaacctgat
     6541 ttcacgttac atcatccgcc aaatggcggt tatggcggtt tgcgcgctcc ttgccttcct
     6601 cgctttgtac agcttttttg aaatcctgta cgaaaccggc aacctcggca aaggcagtta
     6661 cggcatatgg gaaatgctgg gctacaccgc cctcaaaatg cccgcccgcg cctacgaact
     6721 catgcccctc gccgtcctca tcggcggact ggcctctctc agccagcttg ccgccggcag
     6781 cgaactggcc gtcatcaaag ccagcggcat gagcaccaaa aagctgctgt tgattctgtc
     6841 tcagttcggt tttatttttg ctattgccgc cgtcgcgctc ggcgaatggg ttgcgcccac
     6901 gctgagccaa aaagccgaaa acatcaaagc cgccgccatt aacggcaaaa tcagcaccgg
     6961 ccataccggc ctttggctga aagaaaaaaa cagcattatc aatgtgcgcg gaatgttgcc
     7021 cgaccatacg cttttgggca tcaaaatttg ggcgcgcaac gataaaaacg aattggcaga
     7081 ggcagtggaa gccgattccg ccgttttgaa cagcgacggc agctggcagt tgaaaaacat
     7141 ccgccgcagc atcatgggta cagacaaaat cgaaacatcc gccgccgccg aagaaacttg
     7201 gccgattgcc gtcagacgca acctgatgga cgtattgctc gtcaagcccg accaaatgtc
     7261 cgtcggcgag ctgaccacct acatccgcca cctccaaaac aacagccaaa acacccaaat
     7321 ctacgccatc gcatggtggc gtaaactcgt ttaccccgtc gccgcatggg tcatggcgct
     7381 cgttgccttc gcctttacgc cgcaaaccac gcgccacggc aatatgggct taaaactctt
     7441 cggcggcatc tgtctcggat tgctgttcca ccttgccggc aggctcttcg ggtttaccag
     7501 ccaactctac ggcaccccac ccttcctcgc cggcgcactg cctaccatag ccttcgcctt
     7561 gctcgctgtt tggctgatac gcaaacagga aaaacgttga acaaatgccg tccgaacctc
     7621 tcttcagacg gcatttgttt tcattgacac attcccgcag acatatagcc gttccctatt
     7681 atagtggatt aaatttaaac cggtacggcg ttgcctcgcc ttgccgtact atttgtactg
     7741 tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctatacatta cctgtcataa
     7801 cagttccatt tttgttaaaa ctagtctatg atagcggtac aaatgttgtt tacaatattt
     7861 aacgcagatc atttgcaacc cgacaaaaga aaaacggaaa aaggaacaaa gagatgttag
     7921 aagcctatcg taaagccgcc gccgagcgcg ccgccctcgg cattcccgcc ctccctttga
     7981 atgcgcagca aaccgccgat ttggttgagc tgctgaaaaa cccgcccgca ggcgaaggcg
     8041 agttcttggt cgagctgctt gcccaccgtg ttccgcccgg tgtggacgat gccgccaaag
     8101 tcaaagcctc attcctggct gccgttgccg aaggcagcgc atccagcccg ctggtttccc
     8161 ccaaatatgc gaccgaactc ttaggtacga tgctcggcgg ttacaatatc cacgccttaa
     8221 tcgaactctt ggacgacgac aaactcgcgc ccattgccgc caaaggcttg aaacatacgc
     8281 ttctgatgtt cgattccttc cacgacgttc aggaaaaagc cgaaaaaggc aacaaatacg
     8341 cgcaggaagt tttgcaatct tgggcagatg ccgaatggtt cgcctcccgt gccaaagttc
     8401 ccgaaaaaat caccgttacc gtcttcaaag ttgacggcga aaccaataca gacgacctct
     8461 ctcccgcgcc cgacgcgtgg agtcgtcccg atattccgct gcacgcgctg gccatgctga
     8521 aaaacccgcg cgacggcatc acgcccgaca aaccgggcga agtcggtccg attaaattgt
     8581 tggaagaact caaagccaaa ggccatccgg ttgcctacgt cggtgacgtg gtcggtaccg
     8641 gttcttcacg caaatccgcg accaactccg tcatttggca taccggcgaa gacatcccgt
     8701 tcgtgccgaa caaacgcttc ggcggcgtgt gcttgggcgg taaaatcgcg ccgattttct
     8761 tcaacactca agaagactcc ggcgcgctgc cgattgaagt cgatgtttcc gcgctgaaaa
     8821 tgggcgatgt cgtcaatatc cttccttatg aaggtaaaat cgtgaaaaac ggcgagactg
     8881 ttgccgagtt tgaattgaaa tcacaagtat tgctggacga agtgcaagcc ggcggccgta
     8941 tcaacctgat tatcggtcgc ggtctgaccg ccaaagcgcg cgaagccctg aaactgcctg
     9001 cctctactgc attccgcctg ccgcaagcgc ctgccgaaag caaagtcggt ttcaccttgg
     9061 cgcaaaaaat ggtcggtcgc gcctgcggtc tgcccgaagg acaaggcgtg cgtccgggta
     9121 cttactgcga accgcgcatg acgacggtcg gctcgcaaga tacgaccggg ccgatgaccc
     9181 gcgacgagtt gaaagacttg gcttgtttgg gcttctccgc cgatatggtg atgcagtctt
     9241 tctgccacac cgctgcctat ccgaaacctg tcgatgtaaa aacccataaa gaactgcccg
     9301 cctttatttc tacccgtggc ggcgtgtccc tgcgtccggg cgacggcgtc atccactcgt
     9361 ggctcaaccg cctgctgttg cctgatacag tcggtacagg cggcgacagc cacacccgtt
     9421 tccccatcgg tatttccttc cccgcaggtt caggcttggt cgcttttgcc gccgcaacgg
     9481 gcgtaatacc gctcgatatg cccgagtccg tattggtacg tttcagcggc aagctgcaac
     9541 cgggcgtaac cctgcgcgat ttggtgaacg ccatcccgct ttacgcgatt aaacaaggtt
     9601 tgctgaccgt tgccaaagcc ggtaagaaaa acatcttctc cggccgcatc ctcgaaatcg
     9661 aaggcctgcc cgatttgaaa gtggaacaag cctttgaatt gaccgacgca tccgccgaac
     9721 gctccgccgc cggctgtacc gtgaagctca ataaagagcc gattatcgag tacatgaaat
     9781 ccaacgtcgt gttgatgaaa aatatgattg ccaacggcta tcaagatccg cgcgctttgg
     9841 aacgccgcat caaagctatg gaaaaatggc tggcaaatcc caagttgctc gaagcggata
     9901 aagatgccga atacgccgcc gtgattgaaa tcaacatgga cgacatcaaa gagccgatta
     9961 tcgcctgccc gaacgacccg gacgacgtat gctttatgtc cgaacgctcc ggcaccaaaa
    10021 tcgacgaagt gttcatcggt tcttgtatga ccaacatcgg ccacttccgc gccgcctcca
    10081 aacttttgga aggcaagagc gacatccccg tccgcctgtg ggtagcgccg ccgaccaaaa
    10141 tggacgcgaa agagttgtcc gacgaaggtc actacggcgt actcggccgc gccggcgcgc
    10201 gtatggaaat gccgggctgt tcgttgtgta tgggtaacca agcccaagta cgcgaaggtg
    10261 caactgtcat gtccacttcg acccgcaact tcccgaaccg cttgggtaaa aacaccttcg
    10321 tttacctcgg ctcggcagag ttggcggcga tttgctccaa actgggtaaa atcccgaccg
    10381 ttgaagaata tcaagccaat atcggcatca tcaacgaaca gggcgataaa atctaccgct
    10441 atatgaactt caacgaaatc gacagctaca acgaagtagc cgagaccgtg aatgtttgat
    10501 ccccgtcatc cgtatggagt aagggattga ccgcaatgcc gtctgaacaa ccttcagacg
    10561 gcattgcaac attccgctaa cccttctttc cgcaaacgct gcaaatacgg cgttcacgcc
    10621 cccacataaa ggaaacgaca gtgaacctga aaaaccgcca ttttctgaaa cttttggact
    10681 tcacgccgga agaaatcacc acctacctcg accttgccgc cgagttgaaa gacgccaaaa
    10741 aggcagggcg cgagattcag cggatgaaag ggaaaaacat cgccctgatt tttgaaaaaa
    10801 catccacgcg cacacgctgt gcgtttgaag tcgccgcacg cgaccaaggc gcggatgcaa
    10861 cctatctgga accgtccgcc agccaaatcg ggcacaaaga aagcatcaaa gacacggcgc
    10921 gcgtcttagg cagaatgtac gatgccatcg aatatcgcgg cttcgctcag gaaactgtcg
    10981 aagaattggc aaaatatgcg ggcgtacccg tgttcaacgg gctgaccaac gagttccatc
    11041 ccacacaaat gcttgccgac gcactgacta tgcgcgaaca cagcggcaaa cctttgaacc
    11101 aaaccgcgtt tgcctacgtc ggcgacgcgc gttacaacat gggcaattcc ctgctgattt
    11161 taggggcaaa attggggatg gacgtgcgta tcggcgcacc tcaaagcctg tggccgtctg
    11221 aaggcattat cgccgccgca cacgccgccg ccaaagaaac cggtgcaaaa attaccctga
    11281 ccgaaaacgc gcatgaagcc gtcaaaggtg tcggtttcat tcatactgac gtatgggtca
    11341 gcatgggcga gccgaaagaa gtctggcagg aacgcatcga tttgctgaaa gattaccgcg
    11401 ttacgcccga actgatggcg gcatcgggca atccgcaagt caaattcatg cactgcctgc
    11461 ccgccttcca caaccgcgaa accaaagtcg gcgaatggat ttacgaaacc ttcgggctga
    11521 acggtgtgga agttacagaa gaagtattcg aaagtccggc cggcatcgtg ttcgatcagg
    11581 cggaaaaccg tatgcacacg attaaagcgg taatggtcgc ggctctgggc gactgacaga
    11641 actgtgcctg tttaaattca tccgcaacac agataccgtc cgaacacgat gttcagacgg
    11701 tatccatata acaaactgcc tacacgatgt gtaggcagtc ccgtttgaaa acaatcagtt
    11761 tttgtcttgg tcaaccaatt tgttggccgt aatccaaggc atcatggcac gcagtcgtgc
    11821 gccgactttt tcaacttggt ggtcggcatt caaacggcgg cgggcagtca tagacgcgta
    11881 gttgacatta ccctcttgga taaacatttt tgcgtattcg ccggtttgga tgcgtttcag
    11941 ggcattgcgc atggcttctt tgctggaagc attgaccact tcagggccgg taacgtattc
    12001 gccgtactcc gcattgttgg aaatggagta gttcatattg gcaataccgc cttcgaaaat
    12061 caggtcaacg atcagtttca tttcgtgcag gcattcgaag taagccattt caggcgcgta
    12121 accggcttcg gtcagggttt caaaacctgc tttgatcaac tcggccacgc caccgcacaa
    12181 tacggcttgt tcgccgaaca gatcggtttc ggtttcttcg cggaaagtgg tttcaatcac
    12241 accgcctttg gtgccgccgt tggcagccgc ataagacagg gcgatgtctt tggccttgcc
    12301 ggaattgtct tggtaaacgg caatcagaga aggcacgccg ccgccgcgtt tgtattcact
    12361 gcgtacggta tgacccggac ctttgggggc aaccataatc acgtccaagt cggcacgcgg
    12421 aacgatttgg ttgtagtgca cgttgaagcc gtgtgcaaat gccagcgtcg cgccttcttt
    12481 caaattggct gcaacttcgg cgtgatagac ggcaggcatg gtttcgtcag gcagcagcag
    12541 cataacgaca tcggcttctt tggtcgcttc agcaacggtt ttaacaacat ggccggctgc
    12601 ttcggctttt ttccaagaag agccgtggcg cagaccgatc accacgttta cacccgaatc
    12661 tttcaggttg gcagcatggg catgaccttg cgaaccgtaa ccgatgatgg caacggtttt
    12721 gcctttgatc agggacagat cggcatcttt atcgtaatag acttgcattt gatttccttt
    12781 aaggtaaatg gttgtcgaag ccttaaaatg ttgagcggct tcggacgggt taaacagagt
    12841 gtgccgctta aacggcaact tcattcatca atacgatttc caacgcttcg gttttgccgt
    12901 cgacggactg gacgaaggct tggaaatgcg cgctggcatt atgttcgtca atagctgctt
    12961 gggatttcca attttccacg aaaacaaaac ggttcggttt gccgatttcc tgatggagat
    13021 tgtagctgat gttgccctct tccgcacggc tggctttgac cagttcttta aactgtgctg
    13081 ccagtgtttc cgtgtattcc ggtttgacgg taaccagtgc gacaatttta atgttcgaca
    13141 taaatctctc ctgccgttcg tttttcagac ggcattcaaa taccatgccg tctgaaaggt
    13201 tacggcgtta aattttcaaa atacgctcac cgcgaccgat gccggccgca cctgtacgta
    13261 cggtttccaa aatttgggcg cgtccgaccg tttccaaaaa ggaatccagc ttgtcggtcg
    13321 agccggtaat ttcaatcgta tagctgcggt cggttacgtc gatgatgctg ccccggtaga
    13381 tttcggtcaa gcgtaaaaat tcgtcgcggt ctttgccggc ggcacggact tttaccaaca
    13441 tcagttcgcg ttcgacaaaa cggctttcgt tcaaatcgac cactttaatc acttcaatca
    13501 atttattgag ttgcttggtg atttgttcga taacctgctc gtcgccgtgg gtaacgatgg
    13561 tcatccgtga cagggttttg tcttcggtcg gcgcaaccgc caaagaatcg atattgtaat
    13621 cgcgtgcaga gaacaaaccg accacgcggc tcatcgcacc tgattcgttt tcgatcagaa
    13681 cagataagat atgtcgcatt tgcctctcct tacgcctttc cgtccgtacg catatgcggc
    13741 ggaagtacca tttcgtccaa acctttgccg ttgccgacca taggcatcac attctgtttc
    13801 tggtcggtca ggaagtcgat aaacaccagc ctgtcttttt ggttcaatgc ttccaacaag
    13861 gcaccttcca catcagattt cttgtccacg cggatgccga tatggccgta tgcctcggca
    13921 agtttgacga aatcgggcaa agaatcgaaa taggtttccg actctcgacc gccgtaatat
    13981 atttcctgcc actggcgtac catacccaga taaccgttgt tcagcgtaat gacgttaacc
    14041 ggaatccgat attggaaaca ggtggacaac tcttggatgt tcatctggat cgagccgtcg
    14101 ccggtaatac agaatacgtc ttgatccggg gcggcaagtt ttgcaccaat cgcataaggc
    14161 agacccacgc ccatcgtacc caaaccgccg gagttgagcc attggcgcgg acgttcgaaa
    14221 ggataatatt gagccgcaaa catttgatgc tgccctacat ccgatgtgat gattgccgaa
    14281 ttgccggtaa tctcggcaag cttctgaatc acatattggg gcttgataat ttcgctgccg
    14341 ttgtcaaacc acaagcaatc gcgggaacgc cattcctcta tggttttcca ccatttgccc
    14401 aaagcatctt cagacggcac ggactcttgt ttttgccaca gcgcaaccat ctcggacaaa
    14461 atgtttttca cgtcgccgac aatcggaatg tccgccttca cgcgtttggc gatgctggaa
    14521 ggatcgacat cgatatggat aaccttcttc gccttctcga aaaatttgga cggtacggaa
    14581 accacacggt cgtcaaaacg cgcacctacg gcaagaacga catccgcatt ctgcatggca
    14641 aggtttgcct cgtaagtacc gtgcataccg agcataccga ggaattggcg gtcgccggaa
    14701 ggataagcgc ccaagcccat cagcgtaccc gtgcacggag cacccgtcat tcggacaaat
    14761 cgggtcagct cttcagaagc attacccaac accacgccgc cgccaaaata gacgaccggg
    14821 cgtttggcag atgccaacat ctgcacggcc tttttaatct gaccgatatg cccttgaaca
    14881 accggttgat acgaacggat aaaaatgtct tcctgaggat agctgaattt cgccatcgcc
    14941 tgcgtaacat ctttcgggac atcaagcacc acgggccccg gtcggccgct tgcggcaatt
    15001 tggaacgcct ttttaatggt ttccaccaac tcattgatgt ccgtaaccaa gaaattgtgt
    15061 ttgacgcacg gacgggtaat acccaccgta tcaacttctt ggaacgcatc cgtaccaatc
    15121 agggaattgc ctacctgtcc gctgatgacc accatcggaa tcgaatccgt ataggcagta
    15181 gcaataccgg tcagcgcatt ggtaacgccc gggccggatg taaccaatgc cacgcccacc
    15241 ttaccgctga cgcgcgcata cgcatctgcc gcgtgtactg ccgcctgctc atggcgggta
    15301 agaatgtgtt tgaatttatt gagttggaac agggcatcgt agatttcgat aaccgcaccg
    15361 ccgggataac cgaaaacgta ctcgacgcct tcggctttga gactctgcac tatgatttgc
    15421 gcgcctgata actgcataac gacctctttt atacggtttc aaaccaatag ggacaaaccg
    15481 ctttgccaca gcacctgtaa tgcaattcca ccaagcagcg atttagggta cgcgcattgg
    15541 gggaacacgg caacagacag attatccaat caattggaaa ggaacacaga gtttgtgaaa
    15601 aagagtaaaa acgataacgc aaaccgacag ttcaatcaag aaaaatcttt catcttttaa
    15661 tattttttga aagcggagaa atttttttga aagcggagaa attattgatt gattttaaaa
    15721 gaataaaatc aggagtacct tttttgaaag atggaaattg ttgacagttt gcatgggggc
    15781 agatgtgaaa gtatcaagaa ttttaaaatt tacagggttt cactcgggat attgattgaa
    15841 cttgatttta tttttgatat atcaaaaata ttcccaacca tacttcctga aaatggctca
    15901 ttgcaccgga cggcattgac agaaccaaga gggctaacaa cgacttaaat atattgattg
    15961 tatagtggat taaatttaaa tcaggacaaa acgacgaagc cgcag
//
LOCUS       DAWTHD010000055        15871 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWTHD010000055 DAWTHD010000000
VERSION     DAWTHD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.055"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..81)
                     /locus_tag="WM704_001271"
     CDS             complement(<1..81)
                     /locus_tag="WM704_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFA9340690.1"
                     /translation="MLPSKYEDNTLQIKERSTKRTAPFFCT"
     gene            303..1646
                     /locus_tag="WM704_001272"
     CDS             303..1646
                     /locus_tag="WM704_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFA9340691.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1649..2881
                     /locus_tag="WM704_001273"
     CDS             1649..2881
                     /locus_tag="WM704_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFA9340692.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2874..3650
                     /locus_tag="WM704_001274"
     CDS             2874..3650
                     /locus_tag="WM704_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFA9340693.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3650..4276
                     /locus_tag="WM704_001275"
     CDS             3650..4276
                     /locus_tag="WM704_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFA9340694.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4280..4873
                     /gene="nqrE"
                     /locus_tag="WM704_001276"
     CDS             4280..4873
                     /gene="nqrE"
                     /locus_tag="WM704_001276"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFA9340695.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4887..6104
                     /gene="nqrF"
                     /locus_tag="WM704_001277"
     CDS             4887..6104
                     /gene="nqrF"
                     /locus_tag="WM704_001277"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFA9340696.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6621..7676
                     /locus_tag="WM704_001278"
     CDS             6621..7676
                     /locus_tag="WM704_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9340697.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7702..7917
                     /gene="nqrM"
                     /locus_tag="WM704_001279"
     CDS             7702..7917
                     /gene="nqrM"
                     /locus_tag="WM704_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFA9340698.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8041..8619)
                     /gene="grpE"
                     /locus_tag="WM704_001280"
     CDS             complement(8041..8619)
                     /gene="grpE"
                     /locus_tag="WM704_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFA9340699.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8802..9620
                     /gene="cysE"
                     /locus_tag="WM704_001281"
     CDS             8802..9620
                     /gene="cysE"
                     /locus_tag="WM704_001281"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFA9340700.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9824..11335)
                     /gene="ubiB"
                     /locus_tag="WM704_001282"
     CDS             complement(9824..11335)
                     /gene="ubiB"
                     /locus_tag="WM704_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFA9340701.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11370..11579)
                     /locus_tag="WM704_001283"
     CDS             complement(11370..11579)
                     /locus_tag="WM704_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HFA9340702.1"
                     /translation="MELETVVPPEEGEGEGGGADGEMPEYLELHGGREDDISASSQPE
                     PPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            complement(11824..11970)
                     /locus_tag="WM704_001284"
     CDS             complement(11824..11970)
                     /locus_tag="WM704_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340703.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11987..12325)
                     /gene="erpA"
                     /locus_tag="WM704_001285"
     CDS             complement(11987..12325)
                     /gene="erpA"
                     /locus_tag="WM704_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFA9340704.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12458..13144)
                     /locus_tag="WM704_001286"
     CDS             complement(12458..13144)
                     /locus_tag="WM704_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9340705.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13331..13603
                     /locus_tag="WM704_001287"
     CDS             13331..13603
                     /locus_tag="WM704_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340706.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDTEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13811..15739
                     /gene="dnaK"
                     /locus_tag="WM704_001288"
     CDS             13811..15739
                     /gene="dnaK"
                     /locus_tag="WM704_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFA9340707.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         4130 a         4372 c         3728 g         3641 t
ORIGIN      
        1 ggtacaaaag aacggagcgg ttctttttgt acttctttct ttaatttgca gtgtgttatc
       61 ttcatatttc gagggtaaca tatctgctaa tctggtacag acccttatca aatcaatatc
      121 ccccttccga caattaaaaa cggcttccgc aaggaaaaaa gcggcaaatc tgacaaacag
      181 gtacaattcc tgataaaatc taacactttg ctacattaat ttattcaatg cttttgatgc
      241 cacgcgacac ggtatcaaaa acacgccgtt tttaccttaa ccatcacaag taatgcaaat
      301 ctatgattaa aatcaaaaaa ggtctaaatc tgcccatcgc gggcagaccg gagcaagtca
      361 tttatgacgg cccggccatt accgaagtcg cgttgcttgg cgaagaatat gtcggcatgc
      421 gcccctcgat gaaaatcaag gaaggtgaag ccgtcaaaaa aggccaagtg ctgtttgaag
      481 acaaaaagaa tccgggcgta gtatttactg cgccggcttc aggcaaaatc gccgctattc
      541 accgtggcga aaagcgcgta cttcagtcag tcgtgattgc cgttgaaggc aacgacgaaa
      601 tcgagttcga acgctacgta cctgaagcgc tggcaaaatt gagcagcgaa gaagtgcgcc
      661 gcaacctgat tcaatcaggc ttatggactg cgcttcgcac ccgtccgttc agcaaaatcc
      721 ctgccgtaga tgccgagccg ttcgccatct tcgtcaatgc gatggacacc aatccgctgg
      781 ctgccgaccc tacggtcatc atcaaagaag ccgccgaaga cttcaaacgc ggcctgttgg
      841 tattgagccg cctgaccgaa cgtaaaatcc atgtgtgtaa agcagcaggc gcagacgtgc
      901 cgtctgaaaa tgctgccaat atcgaaacac atgaatttgg cggcccgcat cctgccggct
      961 tgagtggcac gcacattcat ttcatcgagc cagtcggcgc gaataaaacc gtgtggacca
     1021 tcaattatca agacgtgatt gctatcggac gtttgttcgt aacaggccgt ctgaataccg
     1081 agcgcgtggt tgccttgggc ggcctgcaag tcaacaaacc gcgcctcttg cgtaccgttt
     1141 tgggtgcgaa ggtgtctcaa cttaccgccg gcgaattggt tgacgcggac aaccgcgtga
     1201 tttccggttc ggtattgaac ggtgcgattg cacaaggcgc gcatgattat ttgggacgct
     1261 accacaatca gatttccgtt atcgaagaag gccgcggcaa agagctgttc ggctgggttg
     1321 cgccgcagcc ggacaaatac tccatcacgc gcaccactct cggccatttc ctaaaaaaca
     1381 aactcttcaa gttcacgaca gccgtcaacg gcggcgaccg cgccatggta ccgatcggca
     1441 cttatgagcg cgtaatgccg ttggacatcc tgcctacctt gcttttgcgc gatttaatcg
     1501 tcggcgatac cgacagcgcg caggctttgg gttgcttgga attggacgaa gaagacctcg
     1561 ctttgtgcag cttcgtctgc ccgggcaaat acgaatacgg cccgctgttg cgcaaagtgc
     1621 tggaaaccat tgagaaggaa ggctgattat gggcttaaaa cattttctgg aaaaaatcga
     1681 accgcacttc ctgccgggcg gcaaacatga aaaatggtat gccctctatg aagctgcggc
     1741 gacgattttc tatacatccg gcgcggtaac gcgcaaagcg gcgcacgtcc gcgacgcgct
     1801 cgactccaaa cgcatgatga ttttggtgtg gctggctttg ttccccgcca tgttttacgg
     1861 catgtacaac gtcggcgcac aggcattcgg tgccttaacg cccgatttgc tgcaacaaag
     1921 catcgccaac gactggcatt acgcccttgc caacgctttg ggcatcaata tgtcgcctga
     1981 agcgggcgtg ttgggcaaaa tgctgttcgg cgcgatttac ttcctgccga tttacgcgac
     2041 cgtatttatt gtgggcggct tctgggaagt cttgttcgca tccgtacgca aacacgaaat
     2101 caacgaaggt ttcttcgtta cttcgattct gtttgcctta atcgttccgc ccacgctgcc
     2161 gctgtggcag gcggctttgg gtatttcttt cggcgttgtg gttgcgaaag aagtattcgg
     2221 cggtacaggt aaaaacttca tgaaccctgc gctggcaggc cgcgccttcc tgttcttcgc
     2281 ctaccccgcc aacttgagcg gcgatgcggt ttggacggcg gttgacggct attccggcgc
     2341 aaccgcgctg gcgcaatggg cggcacacgg tgcagacggc ctgaaaaacg ccgtaaccgg
     2401 tcaaaccatc acttggatgg acgcatttat cggcaaactg cccggctcca tcggcgaagt
     2461 ctccactttg gcactcttaa tcggcggcgc gtttatcgtg tttgcccgca tcgcttcttg
     2521 gcgcattatt gccggcgtga tgatcggtat gattgcgatg tcttcgctgt ttaacttcat
     2581 cggttctgac accaacgcta tgtttgctat gccttggtac tggcacttgg tggtcggcgg
     2641 cttcgccatc ggtatgctgt ttatggcgac cgaccccgtt tccgcttcct ttaccaatgt
     2701 cggcaaatgg tggtacggcg cgcttatcgg cgtaatgtgc gtgttaatcc gcgtggccaa
     2761 tccggcttac cccgaaggca tgatgttggc gattctgttt gccaacctgt ttgccccgat
     2821 tttcgactat ttcgtcgcac aagcgaacat caaacgcaga aaggcgcgca gcaatggcta
     2881 agaaattcga taaagacagc ttcagcggca cgctgattgt cgtgttggcg gtcagcctga
     2941 tttgctcggt catcgttgcc ggtgcggtcg tcggcttgaa acccatccaa gaaaaacaaa
     3001 aactccaaga caaacaaggc tatattttga gcgtggccgg tttgatggat aaggacaccg
     3061 acatcggtaa aacctttgcc gagcgtatcg agcaacgcgt ggtcgatttg gcgaccggcg
     3121 aatatgtgaa agacgcgccg aaagacttca gcgcgcgcat cgcaggcaaa gaccccgcgc
     3181 aaagcatccg catcaaacct gaagacgatt tggcaggcat caaaagccgc gccaaataca
     3241 ccgaggttta tttggtaaaa ggcgaagacg gcaaaatcgg gcaaatcatc ctgccgatgc
     3301 acggcaacgg tttgtggtcg gtgatgtacg gcttcgtcgc catccgaccc gacggcaaca
     3361 ccatcaacgg cattacctac tacgaacaag gcgaaacccc gggcttgggc ggcgaaatcg
     3421 gcaatccttt gtggcagcaa aaattcgtcg gcaaaaaatt gtttgacgga caaggcaaac
     3481 tcgccctgca cgtgggcaaa ggcgcgggtt cggacaaaga acacggcgta gacgccctct
     3541 ccggcgcgtc gctgacttcc aaaggcgtgc aaggttcgtt cgcctactgg ttcggcgaaa
     3601 acggctatat cccctacctg aacaaattga aatcagcagg agtgcaataa tggctgatat
     3661 gaaacgcttg aaacatttga tgttctcacc ctttatcgac aacaacccga ttgccttgca
     3721 ggttttgggt atttgttcgg ctttggcggt taccaccaaa cttcagacgg ccatcgtaat
     3781 gggtatttcc gtagctttgg taaccggttt ttccagcttc ttcatttcgc tggtgcgcaa
     3841 ctacatcccc aacagcatcc gcatcatcgt gcaaatggcg attatcgcgt cgctggttac
     3901 gctggtcgac caactgcttc aggcatttgc ctacgaattg tccaagcagc tttccgtatt
     3961 cgtcggcctg attattacca actgtatagt gatgggccgt gccgaagcat ttgcgatgaa
     4021 agagccgccg ctggaaagcc tgatcgacgg catcggcaac ggtgcgggct acgggatgat
     4081 gctgcttgtc gtcgccaccg tccgcgaact gatcggctca ggcaaacttt taggttatac
     4141 cgtctttcaa accgtacagg acggcggctg gtatcagacc aacggcctct tcctactcgc
     4201 ccccagcgcg ttcttcatca tcggcttttt gatttgggga ctgcgtactt ggaaacccga
     4261 acaggcggag gaataagcta tggaacacta tttgagcctc ttcatcaaat ccgtcttcat
     4321 tgaaaatatg gcgctatcct tctttttggg tatgtgtacg tttttggcgg tatccaaaaa
     4381 agtatccacc gcattcggtt tgggtgtggc ggtaattttc gtactcgggc tgtccgtccc
     4441 cgccaaccaa ttggtttact cgctgctcaa agacggcgcg attgtcgaag gcgtggattt
     4501 gacctttttg aaattcatca cctttatcgg cgtaattgcc gctttggtgc agattttgga
     4561 aatgttcttg gacaaattcg tccccgccct ctataacgcg ctgggcatct acctgcctct
     4621 gattaccgta aactgcgcga ttttcggcgc cgtatcgttt atggcgcaac gcgaatacga
     4681 cttcggcgag tccgtcgtgt acggcttcgg cgcgggtttg ggctggatgt tggcgattgt
     4741 cgctttggcg ggcattaccg aaaaaatgaa atattcggac gctcccaaag gcctcaaagg
     4801 cttgggcatt acctttatcg ccgccggcct gatggcaatg gcgtttatgt cgttctccgg
     4861 catccagtta taagaaaggg accggtatgg agattatttt aggtatcgtg atgtttaccg
     4921 tcatcgtctt agctttggca ctgatgattc tgtttgccaa atccaagttg gtcagcgaag
     4981 gcgacatcac catcaaagtc aatgatgaaa aagaactgac tatgcccgcc ggcggcaaac
     5041 tcttgggcgc gcttgccagc caaggcatct ttgtcccctc cgcctgcggt ggcggcggtt
     5101 cgtgcggaca atgccgcgtt gtcgtgaaaa gcggcggcgg cgacattctg ccgaccgagc
     5161 tgtcccacat cagcaaacgc gaagcacgcg aaggctgccg tttgtcctgc caagtcaatg
     5221 tcaaaaccga catggacatc gaagtacccg aagaagtgtt cggcgtgaaa aaatgggaat
     5281 gcaccgtcat ttccaacgac aacaaagcaa cgttcattaa agaactcaag cttgccattc
     5341 ccgaaggcga agaagttcct ttccgcgccg gcggctacat tcaaatcgaa gccccgccgc
     5401 acaccgttgc ctacaaagac ttcgacattc ccaaggaata tcacgaagac tgggacaaat
     5461 acaatctgtg gcaatacgtt tccaaagtgg acgagccgat tttgcgcgcc tactctatgg
     5521 cttcgtatcc tgaagaaaaa ggcatcatta tgctgaacgt gcgtatcgcc acgcctcccc
     5581 cgcgcgtacc cgatgcgcct ccgggacaaa tgtcgtccta catctggtcg ctcaaacccg
     5641 gcgacaaagt tacgatttcc ggcccgttcg gcgaattttt cgccaaagac accgatgccg
     5701 aaatggtatt tatcggcggc ggtgcgggta tggccccgat gcgttcccac attttcgacc
     5761 agttgaaacg tttgcactcc aaacgcaaaa tcaccttctg gtacggcgca cgctccaaac
     5821 gcgaaatgtt ctatgtcgaa gacttcgacc aactcgcggc agaattcccg aacttcactt
     5881 ggcacgtcgc cctgtccgac ccattgcctg aagacaactg ggacggctac acgggcttca
     5941 tccacaacgt ggtttacgaa aaccacctga aaaaccacga agcaccggaa gactgcgaat
     6001 tttatatgtg cggcccgccg attatgaacc agtccgtcat caaaatgctc aaagacttgg
     6061 gcgtggaaga cgaaaacatc ctcttggacg atttcggcgg ttaaatattc ggcttcaaac
     6121 acaaaaggaa gaaaccccgg tttcttcctt ttttatccga tgccgtccga acacccttca
     6181 gacggcatcc gcccataccc gtatcaatat cgttttttta ccctgcggcg gatgtgcggc
     6241 taaatatttt gacgaaaaaa caaagaagta tacttctttt tagttatggt tgattccata
     6301 actaaaaaag tatcgatcaa cccattttac gaggtacgga taatgtctga cagctttgaa
     6361 aacagccccg aatacgatga ttggattgaa tcgggcggtc gtgatgaaga ttacgaatac
     6421 tactacaaca aatggcaacg caggacgcgc tgaccattaa accctgacaa accggtttgt
     6481 aaaacaccgg tttgtttttt attaccgcca cccaaacgac gggcgatgcc gcccgaacgc
     6541 ccttcaaacg gcatcggcat agaaacatac cggccgtttt gcgatataat tcaagccatt
     6601 tgccaaaacc gtcaaacact atgccgtctg aaacacgcct gccgaacctt atccgcgcct
     6661 tgatatttgc cctgggtttc atcttcctga acgcctgttc ggaacaaacc gcgcaaaccg
     6721 ttaccctgca aggcgaaacg atgggtacga cctataccgt caaatacctt tcaaataatc
     6781 gggacaaact cccctcccct gccaaaatac aaaagcgcat tgatgatgcg cttaaagaag
     6841 tcaaccggca gatgtccacc taccagaccg attccgaaat cagccggttc aaccaacaca
     6901 cagccggcaa gcccctccgc atttcaagcg atttcgcaca cgttaccgcc gaagccgtcc
     6961 gcctgaaccg cctgactcac ggcgcactgg acgtaaccgt cggccctttg gtcaaccttt
     7021 gggggttcgg ccccgacaaa tccgttaccc gtgaaccgtc gccggaacaa atcaaacagg
     7081 cggcatctta tacgggcata gacaaaatca ttttgcaaca aggcaaagat tacgcttcct
     7141 tgagcaaaac ccaccccaaa gcctatttgg atttatcttc gattgccaaa ggcttcggcg
     7201 ttgataaagt tgcgggcgaa ctggaaaaat acggcattca aaattatctg gtcgaaatcg
     7261 gcggcgagtt gcacggcaaa ggcaaaaatg cgcacggcga accgtggcgc atcggtatag
     7321 agcaacccaa tatcatccaa ggcggcaata cgcagattat cgtcccgctg aacaaccgtt
     7381 cgcttgccac ttccggcgat taccgtattt tccacgtcga taaaaacggc aaacgcctct
     7441 cccacatcat caatcccaac aacaaacgac ccatcagcca caacctcgcc tccatcagcg
     7501 tggtctcaga cagtgcaatg acggcggacg gtttatccac aggattattt gttttaggcg
     7561 aaaccgaagc cttaaggctg gcagaacaag aaaaactcgc tgttttccta attgtccggg
     7621 ataaggacgg ctaccgcacc gccatgtctt ccgaatttgc caagctgctc cgctaaacat
     7681 acaaaccgat taggaaacac catgaaaacc ctgctcctca ccttcggcat cttcctgacc
     7741 gtcatcatcg gtatggcggt cggctatatt ttctccaaac gcaccatcaa aggcagttgc
     7801 ggcggcatta ccgctttagg tatgaaaaaa atgtgcgact gcgacacacc ttgcgacacc
     7861 ctgcaaaaaa agctggatga agaaaaacag gcaggcggca tacgggttga ccgttaacgc
     7921 cgaaacaaaa tgccgcctga agcccggccg gcgggttcag acggcattgc cgctttactt
     7981 cgatcggttt atacgcaaaa cgcgcttcag gaaataaatc agattattcc ccagacgcct
     8041 tcaggcttcc ttctgtgcca ccgtaaccat agccgggcgc aacacacggt cggacagggt
     8101 ataacccttc ttcatcacac caaccacggt attgggttcc tgctcactgg ccaccgcctg
     8161 catcgcctga tggatattcg gatcgagctt atcgcccgct ttaggattga tttccttgat
     8221 ttgcgtggca tcaaacgcct tctgcaactc attcaaagtc atctgcacgc ccattttcag
     8281 cgcatcgaaa ttaccgctct gatccaaaag cgccatttcc agataatcct tgaccggcaa
     8341 catttccacg gcaaacttct gtccggcgaa cttgtgcgta tcggcaattt cctgctggtg
     8401 gcggcggcgc aggttttgct cgtttgccaa agcgcgcagt tgttcgtctt tcaactgcgc
     8461 ttccaactcg gcaatccgcg cctgcaaatc ctcataagcc ggctcggcgg cagcctgttc
     8521 ctgtacaccg tccgcatttc ctactgtctc gacggtttcc accgcctcca cattttcaac
     8581 cgcttcttca ctgttttgct gctgtgtctg ttcgctcata tcgtatccct caaaacaaat
     8641 tggaaatcaa aatccggtta ttacccgagc aagataagga cattttcaag aaacttcaag
     8701 caaaggcagg aaatttcaca tccgccgccg aataccctac cgcaaaaacc atataaacgg
     8761 taagataccg ccgaccttat gcttttcaga cggcatccat tatgaaaaaa aaccatttga
     8821 acacaaccgg tttcgacctc tggcacacca tccgcgaaga aaccgcggcc gctgccgccg
     8881 ccgaaccgat gctggcaagt tttttgcacc aaaccgtgtt gcgccacgag tccctcggct
     8941 ccgtccttgc ctaccacctt tccagcaaac tcggcagccc gattatggac gtgcgcgcgc
     9001 tgtttgaaat ttaccagcag gcattgggca gcgacaccca aatcagcaaa tgtgtcgaag
     9061 cagacttaaa agccatctac gaacgcgatc ccgcctgcga cgaatattcg ctgccgcttt
     9121 tatatttcaa aggcttccac gcgattcagg cacaccgcat caaccaccgg ctgtatctcg
     9181 acggacgcaa aacgctggcg tatttcctac aaaaccgtat gtccgaagta ttcggcgtgg
     9241 acatccatcc cgccgcccgt ttaggatacg ggctgatgct cgaccacgcc accggctttg
     9301 ttgccggaga aaccgccgtg ttgggcaaca atatttcgat tttgcacggc gtaacgctcg
     9361 gcggttcggg caaagaaggc ggcgaccgcc accccaaaat cggcgacggc gtgatgatcg
     9421 gcgcaaacgc ctcgatattg ggcaatatcc gcatcggcag caatgccaaa atcggcgcgg
     9481 gcagcgtcgt ggtttcagac gtgccgccgt ccatcacggt tgtcggcgta cccgccaaac
     9541 ccgtggcgcg atcgctcaaa accccgtcgg cggatatgga tcaaaatatc cagtttgccg
     9601 aaatcgactt tatgatttga atatccgcac caatgccgtc tgaaacgcca aacgggcttc
     9661 agacggcatt gcccgcttca agcctgcgcc ttaatatgcc gccgatgccg gcacttatca
     9721 gtcggacaaa atgcctttta ttcgatacgc ccgattccca cgcaaccgga aataaccggt
     9781 ttccttgtaa acgggcggag tcgggcaatg attcggacaa agcctatttc aaaagcaaaa
     9841 tcagcagcaa aacaaccgct atcgctgcca gccacaggct ttgccgctgc tgcactttga
     9901 ccaaatggat ataggcatcg cgcatttcct gccggcgcgc ctcatcgacc aaggcattga
     9961 ttttgcgcgg cagggaaggg atgatttgcg cccagtctgg ggcttcgttt ttgaggttgc
    10021 gccaaagggc tttgggaccg acctgcccgt tcatccattt taccaaaaac ggtttggcgg
    10081 ttttccacaa gtccaaatcg ggatcgagtt ggcggcccaa gccttcgatg ttgagcagcg
    10141 ttttttgcag caataccagc tgcggctgga tttcgacatt gaagcggcgg ctgacttcaa
    10201 acaggcgcat cagtaccaag ccgaaggaaa tctgcgaaat cggtttgttg aacacaggct
    10261 cgcacacggc gcgaaccgcc gcttccaact cttccgcacg cgtgtcggca ggcacccagc
    10321 ccgattcgac gtgggcggtg gcgacacgtc ggtaatcgcg gttgaaaaag gcgaggaaat
    10381 tgatggcgag atagcgtttg tcgtaatcgg tcagcgtacc gacgatgccg aaatcaaggg
    10441 cgatatagcg gttgtcggcg gcaaccaaaa tattgccggg gtgcatatcc gcgtggaaaa
    10501 agccgtcgcg gaaaacttgc gtgaaaaaga tttccacgcc gtaatcggca agtttgtgca
    10561 aatcgatgcc gtctgctttg agtttggcga tgtcggaaac cggcgtgccg tccatccatt
    10621 caatcgtcag cacgtcgctt gtgcagtagt cgtaaaacac cttgggcaca atcagcatat
    10681 tgctgttttg gaaattgcgc ccgagttgtc ctgcattggc ggcttcgcgc atcaaatcca
    10741 attcgtcgtg caggtatttg tcgaactccg ccaccacttc gcgcggcttc agacgcttgc
    10801 cgtctgaaaa caagcgttcg acccaagccg cgccgaaacg catcagcgac aagtcttgtt
    10861 cgataagggg caagaggttg gggcgcaaaa ctttgaccgc cactcgttcg cccgaatgca
    10921 ggcgggcttt gtgtacttgg gcgatggacg cgctggcgac gggctcggtt tcaaattccg
    10981 catacagctt ttcgatgggc tgacccagcg atttttcgat ttgttcacgc gaaagccgcg
    11041 cgtcaaaagg cggcactttg tcttgcagct ttgccagttc gacggcgtaa tcgtgcggaa
    11101 tcaaatcggg gcgtgtggac aaaacctgcc cgaacttgat gaaaatcggc cccaggcttt
    11161 ccaaggcaag gcgcagacgg acggcaggcg gttcgttttt caatttggac gactgcggca
    11221 tcattttcag cagagcgcat atccaaccgc tacccatcag cgaaacgcac agacctgcca
    11281 gccgatagcg gtaaaaagtc ccgacaatga ccgtcaggcg tttcaaccat ttcatatata
    11341 aaatcccgaa tacagcattt atcggacaat caaaaaatct taccgagcag atagcccaaa
    11401 aaggcaatca gcaacccgaa aaccagcggt ttgatattgc ggggcggctc gggttgcgaa
    11461 gatgcggaaa tatcatcttc ccgcccgccg tgaagttcaa gatattcggg catttcccca
    11521 tccgcgcctc ctccttcgcc ctccccctcc tccggcggca cgaccgtttc caactccata
    11581 tacgggtaat aatatccaaa cccaaatagc tgaaataatc atgcaactct tcccgtttcg
    11641 cccaagtgca tttttcctca accagcactt gcggtttgga atcgagcgat tcgatcaatc
    11701 cttccgcctt ttcttcgccg agattctcct taaggcactc ccggataagc ccgcgatcca
    11761 cttggggcgg gtaggaaaca tatacatcgt acaaatcgtt tcggtaatct ttactcatct
    11821 caatcataag ggaacaaata cgcctgatac cgttccgccc gttcctttcg ggaaacccga
    11881 ttcatcttca agcaatcgga ttatatgtaa attcatcccg ataatatatt ttccaaaagt
    11941 aaaaatgccg tccgaacgtt cagacggcat aacacaaacc agccggtcac acggaaaacg
    12001 acgatccgca accgcaggtt gtttccgcgt tcggattgcg gatgacgaac tgcgaaccct
    12061 gcagactttc cgtatagtcg atttccgcac cgaccagata ttgatagctc atcggatcga
    12121 ccaaaaagac caaaccgttt ttctcaattt caaaatcgtc gtcgtttttg atttcgtcaa
    12181 aagtaaatcc gtactggaaa cccgaacagc cgccgccgtt gacaaaaacc cgcaatttca
    12241 aatcgggatt gttttcttcg gcaatcaaat cggcaacttt ggcacagcag ctgtcagtaa
    12301 aaataatagg gctttcgtcc gacatagtgt tattccttaa tataatattt tctgcattct
    12361 ctctcaaccc cccttcaaag gcaagcaggg cttcgccttt gaaaaaggaa ttgtcccaat
    12421 agataagttt gaccgcctgc aatttcaagc cccaaactca aaccgtcctg ccgaaccact
    12481 cgacacgccc gataatctcc acatcatcgg tcaagtcgtt caaattgatt tcaaaagcag
    12541 gataagcctc gttggcggaa atgacattga taatcccgcc ggggacgatt tgcaggcgtt
    12601 tgaccaacag attttcatta atccgcaaca catacagtcc gtctctcggc gtattttccc
    12661 catgattgac caaaatcgaa tctccgtcat tcaaaacccc ctccatcgaa tcccccttga
    12721 cggaaattac agacaggttt ttcgtatctc gggtaacata attctcaatc caatgccgtc
    12781 tgaacgccat tgtaaatacc ggttcctcat gaccgacaaa ctgcccgtat cccgcagccg
    12841 cccgaatatc atatctcggc acaaagacaa actcgtccgt atcaacttca tttcccaagg
    12901 tatcgtaagc aagggatttt tttggggctt catccggaaa cggattaccc tcccctgtca
    12961 gcagccaatc gatactacac cccttcaact gcttaatttt tttcaatgtt tcagacttcg
    13021 gcagaccgcc ttcattccat attctgctga agcccgcaat cgtcatttca atatcggatg
    13081 cgatttttgc ctgcctcgct tcacttttcc ataaaaaaac cagtctgtct ttaaaggttt
    13141 ccataaatat ccttccgctc cccttcattc aatcatctaa agtaaacatg aaatatattt
    13201 tacattagct atcccaaaaa cggaataact atgataaact aaatttaaat tatgattttt
    13261 cttatccaat acataatttt tcagattttc cataggttta atcccgaatt gacagaaaaa
    13321 ggatagaaaa atgaagaaaa ccagcaaata tcttatctat actgcggcat ttacctcatt
    13381 ctgctttgcc ttccaagaaa accgttctga agccaaacag cccgacatca ctttatccgc
    13441 atccctgtgc gaacaattca acatgctgaa cgccaaagat atggatacag aacaagtctc
    13501 cctttccaaa gaatgcgaca tcatcgagtc ttcacacgac tgggaaaaag agtacggcaa
    13561 cttgaacgaa caggaaatgc tcgccggcgt cgtctatgaa taaacctgcc tgccatttga
    13621 aacattatgc ttgaatgcat tggagccaaa tgtattaaat caaatataaa accaatatat
    13681 tcataaagtt atatacttat agccatgctg tagcttgaaa cagcccgccg cccgccctat
    13741 ttacagccca tcgggacaaa atgttccaac atatttctca aaataagcaa aatcaaatag
    13801 gagtatccac atggcaaaag taatcggtat cgacttaggt acaaccaact cttgtttggc
    13861 catttccgaa aacggtcaaa ccaaagtcat cgaaaacgca gaaggcgcac gcaccacgcc
    13921 gtccattatc gcttatttgg acggcggcga aatcctcgtc ggcgcgcctg ccaaacgcca
    13981 agcggtaacc aacgccaaaa acactattta cgccgccaaa cgtttgatcg gtcacaaatt
    14041 tgaagacaaa gaagtccaac gcgacatcga atctatgcct ttcgaaatca tcaaagccga
    14101 caacggcgac gcatgggtaa aagcacaagg caaagagctg tctcctcctc aaatttccgc
    14161 agaagtcctg cgtaaaatga aagaagccgc cgaagcttac ttgggcgaaa aagtaaccga
    14221 agccgtgatt accgtccctg cctacttcaa cgacagccaa cgtcaagcca ccaaagacgc
    14281 aggccgtatc gccggtttgg acgtaaaacg catcatcaac gagccgaccg cagccgcttt
    14341 ggcattcggt atggacaaag gcgacaacaa agaccgcaaa atagccgtat atgacttggg
    14401 cggcggtacc ttcgatattt ccatcatcga aatcgccaac ctcgacggcg acaaacaatt
    14461 tgaagtattg gctaccaacg gcgatacttt cttgggcggt gaagacttcg accaacgctt
    14521 gattgactac atcattgacg agtttaaaaa agaacaaggc attgatttga aacaagacgt
    14581 aatggctctg caacgtctga aagaagctgc cgaaaaagcc aaaatcgaat tgtccagcgg
    14641 ccagcaaacc gaaatcaacc tgccgtacat taccatggac gcaaccggcc cgaaacactt
    14701 ggcaatgaaa attacccgcg ccaaattcga gagcctggtt gaagacctga ttgcccgctc
    14761 tatcgagcct tgccgcaccg cattgaaaga tgccggcttg agcaccggcg acatcgacga
    14821 cgtgattttg gtcggcggtc agtcccgtat gccgaaagta caagaagccg ttaaagactt
    14881 cttcggcaaa gaaccgcgca aagacgtgaa ccctgacgaa gccgttgccg taggtgcagc
    14941 gattcaaggc gaagtattga gcggcggccg cagcgacgta ttgctgctgg acgtaacccc
    15001 tctgtccttg ggtatcgaaa ccatgggtgg tgtgatgacc aagctgattc aaaagaacac
    15061 tactattccg actaaagcgt ctcaagtgtt ctctactgcc gaagacaacc aaagcgcagt
    15121 aaccatccac gtactgcaag gcgaacgcga acgcgcttct gccaacaaat ctttaggtca
    15181 attcaacttg ggcgacatcg cacccgcacc acgcggtatg ccgcaaattg aagttacttt
    15241 cgacatcgac gccaatggta tcttgcacgt ttccgccaaa gacaaaggca ctggcaaagc
    15301 ggccaacatt accatccaag gttcttcagg tttgagcgaa gaagaaatcg aacgcatggt
    15361 gaaagatgcc gaagccaatg ccgaggaaga taaaaagctg actgaattgg tcgcttcccg
    15421 caaccaagcc gaagccctga ttcactccgt gaaaaaatct ttggcggact acggcgacaa
    15481 actcgacgct gccgagaaag aaaaaatcga agccgcgctg aaagaagccg aagaagccgt
    15541 gaaaggcgac gacaaaaccg ccatcgatgc caaagccgaa gcactgggta cagccagcca
    15601 aaaactgggc gaaatggttt acgcgcaagc gcaagccgaa gcccaagccg gcgagggcgc
    15661 acaagccaat gcttctgcaa agaaagacga tgatgtcgta gatgccgact ttgaagaagt
    15721 aaaagacgac aaaaaataat tgatgccgtc tgaaaaaaac gcgaaccatt cggttcgcgt
    15781 ttttttcaat tgagataaaa gacaatagca tgacagagat tccaaattca tctaccgtga
    15841 tattcccaag cccaagttcc aactgttgca t
//
LOCUS       DAWTHD010000056        15549 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWTHD010000056 DAWTHD010000000
VERSION     DAWTHD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15549
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.056"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            334..753
                     /locus_tag="WM704_001289"
     CDS             334..753
                     /locus_tag="WM704_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340708.1"
                     /translation="MNETDLPKLLSVINRFTDIDKNWSCVLLFWIQRASGYLEMMTLD
                     DDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEILFLMAQYGSEYWNSLEESF
                     EEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            750..3302
                     /locus_tag="WM704_001290"
     CDS             750..3302
                     /locus_tag="WM704_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFA9340709.1"
                     /translation="MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQN
                     IVNDRFRILNAHELIQVLDLSPQISGIKMNLGLSDENWSKDALPFLEKYIAFVQRLPA
                     SESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKTEEIAKKTAVWRYGIMCAALL
                     HDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKSAYSTHAEI
                     AWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLR
                     SGSRQRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNV
                     PDYIRQYLRKNQHPAAGSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMD
                     GEKVRNIFSVLCFNAKTLYPDGDYPTEFLGNLSEVSAKKEIPDVVVSEDKVSTQIQGA
                     EDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAFDNSMNTAAPAENKSSESE
                     KAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEVKTETVD
                     KPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTH
                     QIDSEAVSLPESESAQNGMRELEQLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAE
                     LEAIVSERNLKKQSETESTEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRS
                     GATVHFIEQGMLLVTPAVFRDYAGGVFNKSDTESLGPLTQKGFEALRLHARTKKSSLH
                     RVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLDESDLLVMEQK"
     gene            3299..5341
                     /gene="traD"
                     /locus_tag="WM704_001291"
     CDS             3299..5341
                     /gene="traD"
                     /locus_tag="WM704_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFA9340710.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSNKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            5331..6026
                     /locus_tag="WM704_001292"
     CDS             5331..6026
                     /locus_tag="WM704_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFA9340711.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWSESVGHDAFPGKEVDDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPAYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(6471..6983)
                     /locus_tag="WM704_001293"
     CDS             complement(6471..6983)
                     /locus_tag="WM704_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340712.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYILPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(7074..7469)
                     /locus_tag="WM704_001294"
     CDS             complement(7074..7469)
                     /locus_tag="WM704_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9340713.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGLL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(7456..9378)
                     /locus_tag="WM704_001295"
     CDS             complement(7456..9378)
                     /locus_tag="WM704_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9340714.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(9455..9826)
                     /locus_tag="WM704_001296"
     CDS             complement(9455..9826)
                     /locus_tag="WM704_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFA9340715.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            10022..11329
                     /locus_tag="WM704_001297"
     CDS             10022..11329
                     /locus_tag="WM704_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFA9340716.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            11322..11756
                     /locus_tag="WM704_001298"
     CDS             11322..11756
                     /locus_tag="WM704_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340717.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(11932..12201)
                     /locus_tag="WM704_001299"
     CDS             complement(11932..12201)
                     /locus_tag="WM704_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFA9340718.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(12383..14845)
                     /gene="lon"
                     /locus_tag="WM704_001300"
     CDS             complement(12383..14845)
                     /gene="lon"
                     /locus_tag="WM704_001300"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFA9340719.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            15097..>15549
                     /locus_tag="WM704_001301"
     CDS             15097..>15549
                     /locus_tag="WM704_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFA9340720.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
BASE COUNT         4016 a         3616 c         3783 g         4134 t
ORIGIN      
        1 ggaatacgat aagggtaaag gctcttatcc aaaagccaaa aggccgtctg ctaaaaaaaa
       61 tcgcagccgg ccttttttaa aagctgtaac ggtaaagcaa cagccttaat ggttaaagga
      121 agggttcaag ggaaaaaggg taaaagttaa aggtaaaaca aaggtctgca tgtaaaaatg
      181 caggcctttg cctattaaaa ggggcagcat tataccttat aaatcttatt ctgtgacaat
      241 tctaattaaa ataacatttt ttattttgat ttttgacatg gcaacttttt tttattatat
      301 gaatggtttg acaaccctgt ctttttaatc aaaatgaatg aaacagatct tcccaagctt
      361 cttagtgtca taaatcggtt tacggatatc gataaaaact ggtcttgcgt gttgttgttc
      421 tggatacagc gtgctagcgg ttatctggaa atgatgacgc tggatgacga tgaaaatacg
      481 gcaaccttcc ttatcgaaaa actagagaaa ctactagagc cactgccgat agatattgat
      541 aacacgactt ttgcagaagc acttgccgat gactttgaaa tcttatttct aatggcccaa
      601 tacggttctg aatactggaa ttcacttgaa gaaagctttg aggaattttg tattagacat
      661 actacgctac ctaatcaatt gattgctgat attccacatg caaaaaagga aaaagtcacc
      721 atgtggctaa aatctttgct taaatcatca tgaaaacaag ccttctcact attgcatcct
      781 cactaatgct tgtcagcggc tcaatgctat acctgataaa tacgagaaaa agtacagatt
      841 ccgtgtcaca taatgatgag gttgcccata tcaatcagaa catagtaaat gaccggtttc
      901 gcattttaaa cgcgcatgaa ctgattcagg tgctcgactt aagccctcaa atatccggta
      961 ttaagatgaa cttagggtta tcggatgaaa actggagtaa ggatgccctt ccctttttgg
     1021 aaaaatatat agcctttgtt caaaggcttc ccgccagtga gtctcatcac catgcaggtg
     1081 atggtggttt agtccgccat acactcgatg tggctgcact ggctcttgtg gcatctacat
     1141 cgcaatcatg gcccccaaat gccaaaaccg aggaaatagc gaagaaaact gccgtttggc
     1201 gttatggaat catgtgtgcc gccctgcttc acgacgttgg taaaactgtg acgggatttc
     1261 aggttgaact gtttgattcg gctatcagtc tcgaaaaatt attatggttg cctgatacag
     1321 gctccatggc tgaaagtggc aagctttatt atcgtgtcga atttcctgat gcaaagtctg
     1381 cttacagtac acatgccgaa atcgcctgga cattctttca agctttggtt ccttcacatg
     1441 tcagacaatg gcttgccaca acggatccga atttaatgat tactttgaga aattatctga
     1501 gtgggaaaaa ggatggcagt cctcttgagc agttaataaa aaatgcggat atgacctcag
     1561 tttcaaggga tttacgaagc ggcagccgtc aacgcttctc aaccgccaag cgtaaaccat
     1621 tcattgaaac cattatggaa acattgaagg agatgttatc cgatagagga gtccatttca
     1681 gcatcgcaac aactgccggt ggtgacctgt tccgtaaggg tgatcgcatc tatttgatgt
     1741 caaaaaacgt acctgactac atccggcagt atttgaggaa aaatcaacat cctgctgcag
     1801 gctcatttcc ggctgacaac caaaggatat ttgacacatt attcgagtac cgtgccgtca
     1861 ttccgccgga aaacgatcct cacagggcaa ttaaccatat agaggttgag tttacccgta
     1921 tggacggtga aaaggtccgt aatatcttca gtgtactttg tttcaatgcc aaaaccctgt
     1981 atccggacgg agattatccg actgagttct taggtaatct ttcagaagtt tccgccaaga
     2041 aggaaatacc ggatgttgta gtatcagagg ataaagtgtc aactcagata caaggtgccg
     2101 aagataaccc agtcactatg accaaggaca aaatggactt cctgtctatc caaccggtga
     2161 cagcagaaca acctccccta cctgaaaaaa atcaaactga gacagctaac tcggcttttg
     2221 acaacagtat gaatactgct gctccagcgg aaaataaatc tagcgagtca gagaaagcta
     2281 aagaaccggg ttacggcaca attgataatt taattgaaaa ctttaacctg atagaagctg
     2341 attcagaaca aacagaatca accaaatcag aagccgagac agcagaaaat gccgttaacc
     2401 tggttcaaga aaatataaga gtagaagtta aaactgagac agtggacaaa cctcgcgctg
     2461 aggttaaaga aactgagaca gataaagcac caaaaacaat taaatcagga accaaagcaa
     2521 gccgtaagaa acttgccggc cttttcgcag acaatcgtaa acctattgga aaaacagccg
     2581 aacccgatgc acagattaaa acaactgaga cagtaactca tcaaatagat tctgaagcag
     2641 tttcattgcc tgaatctgaa tcagcacaaa acggtatgcg tgaactggag cagctaagac
     2701 taagagaacc tacatctagt ccacgcccgg ttcaagtcat ggatgactcc gaaggtttag
     2761 atagccttgc aagcggggta aaaaatatgg ccgaactcga agccatagtt tctgaacgta
     2821 atttgaaaaa gcaatcagaa acagagtcga ccgaaatagt cgaggattca gtcaaagcca
     2881 aacatatgga ggccagagac cgtggaatgc agtttttacg atggctggca gatggattag
     2941 gagacggcag catagcagtc aatagaagtg gtgcaacagt ccactttatt gaacagggga
     3001 tgcttttggt tacgccagcc gtatttcgag attacgccgg cggcgtgttc aataaatctg
     3061 atacggaaag tctaggccct cttacacaaa aaggctttga ggctttgagg ttgcatgcaa
     3121 gaacgaaaaa aagctcattg catagggttc tgactacaaa cggtagcaat cgcagactct
     3181 tctattgcta ccttatccct gaacataata tcaaacatat catacagcca ggttcaagac
     3241 ctcagaataa taccgatatt aaattagatg aatccgactt attagtaatg gaacaaaaat
     3301 gagtgcccac ttccctgaaa acgtttatcg atcaccctat gaatggatca gtgtagccgc
     3361 atactccacc acagcggttt cagtagcaca tttgcccgta cctccgacaa ttgcttatgg
     3421 cagtttagct attttttcgt tggtttcagt caaacgtgcg ttatcagcat tacgcattat
     3481 gcgtttccgt aaaaatattc ggaagttgcc ctattatgcc cttactgccg atgaaattcc
     3541 ttcttctaat aaagctcttt ttcttgggaa aggttttaga tggacacaga cacataccca
     3601 acgtcttttt atggttcgcc agcctgagaa cgacggactt cgtaacccct caaaactcta
     3661 taaggcagcg cgaaaacatg agattgagac aatgggtaca gactggattt cccgcataac
     3721 tcggaaatcg gcatggtgga atccggttgc tcccttacct ccggtcggag gttctcctga
     3781 attacatggc attgaaattg aggaagatga tatttgggaa gatttggggg agcgtgttgg
     3841 ccacaaggtt gtattgggca caacccgcgt tggtaaaacc cgattatgcg aaaccatgat
     3901 tgttcaggat attcggcggg gggatgttac gatcgcattt gatccaaagg gtgacgctga
     3961 cctgatgact agaatgtatg tcgaatcgca aaaatcaaac aggccgtttt atatgttcca
     4021 tttgggtttc cccgagtaca gttgccggta caaccctatc ggggattatg gccgtattac
     4081 cgaggtggcg acaaggattg caaaccaact gccttctgaa ggtcagtctg ctgctttccg
     4141 agattttgta tggcgtttcg tcaacgtttt aacgaaaacc atggaaggtt tatccattaa
     4201 accaagctat gaaaatatct ataagtctgc tgcaaatgta gatgaacttg caggggatta
     4261 ctttaaaaag atattagata aacaccaccc tggttgggag aaagacttcg acaattttga
     4321 tatgcccgag tctgaagcaa aaagcgcagt caaaactggg cgcaacttgg acacaatgaa
     4381 gctcggttct ttcttgaagg ccaaaaagca taacgaacct cttattgacg cattgggtgg
     4441 tattttgtcc aatgacagat cctattttga gaagctagtc agctccctgt atccattgct
     4501 ggaaaaactg acgactggcg aagttgccaa gcttatcagc ccggatacag aggatttgtc
     4561 agatccaaga atcattatgg actggcgcaa agtcatggaa tcgaatgccg tcgtctatat
     4621 tggattggat tccctatctg atgccgaagt tgcagcagcc gttggtaatg cgatgtttgc
     4681 tgacttaacc tctcttgcgg gtcagatcta taaattcggc catggggttg gacagagtgg
     4741 aaaaacccag aaaagaaaag tctcaattca tgcagacgaa ttcaatgaat taatcggtga
     4801 cgaattcatt ccgttattga ataaggccgg tggtgccggc taccaagtga cggtttatac
     4861 acaaacctgg aaagacgtag aagccaaaat tggttcccct gctaaagcag gtcaaattgg
     4921 cggcaacttg aatacgatga ttatgcttag ggtgaaaact gtcgaaacag cagagatgct
     4981 gacgaatcag ttgccggaag tcaaaattat gaccagcact ctagtctcaa gggctggtga
     5041 tgttgccttc cctgatagtc acgaagattt tagcagtgga aacgaagaca ggattggtgc
     5101 tgagacagtt cctatgctga cacccgccga tttaacccaa ttacccaaag gtcaagcatt
     5161 cgcgcttatt gaaggtggcc agctatataa aattcgactc cctctaccca aaaaggacaa
     5221 gcaggaggaa atccctgcgc atattggaga aatggcggct gatatgcgcg aagtttacag
     5281 actaagccgg ccttcggctg aagaaaacgc atttacggag ggaagtagtt atgccgtcta
     5341 atacaagcgc atggtacatg aattcattgc ctttaaagtt cttatttctc ccgtttaaag
     5401 ctgcttatac cttattctta attgtgctgc ttatctttgg tagttcggtg ttgacacaaa
     5461 actattttgg taccggtaat ctcttggaag atgaaatcaa atggtctgag tctgttggcc
     5521 atgatgcgtt tccaggaaaa gaggttgacg atactataaa tctgcaaatt gccagactta
     5581 gttacaaggg gctgaaaacc gtattttttg acctgacatt agtcaatgaa tcactgaaag
     5641 ccaagtcaga agccgatttt ggttacaaat ttaaaaatta tttagtcccc cgtatgaaat
     5701 atctacagca attcgatact acgttgaagg ttatttcctt acgtttggga aatttggcat
     5761 taatcgtggg tatctcagta atctttgcaa tcccggtttt gatagatggg ttagtgatga
     5821 gagctatcag gcaggaaaat gcctcaaggg agagcgccgg tatttatcac cgtgcaaaat
     5881 attggagaac agggattatt tggttgggtt gcatgattta tatgtgtgtg ccaatatcaa
     5941 taccggccta cttgctctat ttacctctgg tagcagcaat ttggatgatt ttcatgcaag
     6001 ctaaatatct taagaaatat ctctaggctt ccagtggaat ctaaatagtg cttttttagt
     6061 tcattaaacg ggggcttaat gctttaactg acggtacaga aatctcgaaa agcattttgc
     6121 aaaatactta aaataagagt atagtaaaaa tgaaatattt caagtttaac taatagttaa
     6181 caatctagag taaacaatcg taatcgttaa atctgggtat agattgcata atatacatta
     6241 tgcgaactat cggaaacagt tgtacgtgta gccggtattg gggaatacgt tgaccttcaa
     6301 tacgattaca ggcagccgac acacaggata tgccgcccat acttttttaa tccaaaacat
     6361 tatatcaacg ggcgcaaaca gtaaatcgcc caacttatcc caaaaattct cctgcttttt
     6421 catcatttct tctgtcaaat ccttggaacg gtcgatttga aaacacggcc ttacatacgt
     6481 cccaattctc tttgcagtgc ttggatattt tgctgtctgt ccaaaacatt gctttgcaat
     6541 gcgttgattt tttgatggtt gatgttgccg ccttttgcca gacgtgcttg tgataacatt
     6601 ttttgggctt cagtcagggc tttgcgttca ttgcttaatt ctgcttcgag aatggagcgt
     6661 ctgctgttat ttacaggtgc ttgttgaggc ggcgtattgg attttgccgg cttggatact
     6721 gttttgaccg gggctttata tttgacagcc tgtccgccgt ttgacggtga tggtgccggt
     6781 tcgggagttt ggggcaggat atagcgttcg ctgctgtagt tgccgattgg gggcaaatcg
     6841 gttgagtggc agcttttaga cggcttggtg gtgtaaacgg tttctccgtt gattgtgcag
     6901 gtatagattt tggccgcatt cgcacccaat gggcttgaaa tcagggaaag gttgattagg
     6961 attaagggga gttttgattt cataatgtgt ttgatattcg gataataatt tgattttttg
     7021 aggatttttt gtaaggtttt cttggcgttt tatgggcttt tcttttcggt tgtttatttt
     7081 tgattttcct tcggggcttt ggcagcctgt ctgttctttt tcccggcatg ttttttgttt
     7141 ttctttttgt ttcctgtgtg tattttggct tcttgactaa gttttttaat tttcaggcgg
     7201 tatccgcctc cctgatggct gctgatttta ggtaaatcag catcggcaca cgatcctgac
     7261 gggtctgatg tatatacgct tttgccattt gagttgcaat ggtatatgga ggcttgcgcc
     7321 gccgcgccgg aaagggacag gagacccaag gcggcaaaaa gtctgatgtt tataccggta
     7381 aagtaaaaag gcggtggtaa cgtccgaatt ataccgttat tggcaggcaa agataagcac
     7441 cctgcccgcg cttctttatc gctcggcaaa ctgtttctcg atttcagttt caatccgttc
     7501 caattcttct tgacaagcca gccaagtttc ttcgatttgg gcaagctgtg ttttgacttt
     7561 tgccagctcg gataaggtat tttgcaattt ttctttgttt gcttcaaggt aagcgtcttc
     7621 ttgtgttaaa aatgcttcgc atcccgtctg aatttcagaa agctgcacca tttctttttc
     7681 ggctttgtcg attttctgtt gtatcggctt actgcgtcgg gctttttctt gacggatttg
     7741 cgcttcgata cgcttggtat ccttgcggtt ttggctttga gtggaagcgg ctggcgcggt
     7801 tgcggcgttt tcctgtgcca agcgccattg gcggtaatcg ttcaaatcgc cgtcaaagtt
     7861 tttcagacga cctttgtcta tcaagaggaa actgtcggtc gtggcttcaa gcaggctgcg
     7921 gtcgtgcgat acgacgatta atgcaccttg gaaactttgc aacgcgagcg ttaaggcatg
     7981 acgcatatcc aaatccagat ggttggtcgg ttcgtcaagc agcagcaggt tcggcttttg
     8041 ccagataatc atggcaaggg caaggcgggc tttttctccg ccggaaaaag gttcggtttt
     8101 ctgtaacgcc atatcgccga caaaatcgaa gccgccgagg aaatttcgga tttcttgttc
     8161 gcgtacttcg ggagaaagct gctgtatatg ccaaacaggg ctttggtcgg cgcggatggt
     8221 gtcaagctgg tgttgggcga aatagccgat attaagtttt tcggaatgga cgatgctgcc
     8281 ggagagtaaa tcgatttttc ctgccaaagc tttgataaac gtagatttac cgctgccgtt
     8341 gacacccaat agcccgtagc gtgcgccgct ttccagcgac agggtaatgt catgcaggac
     8401 ggttttacct tcgtaaccca aatcagcgtg ctccagcttc agcaaaggat tgggcagatg
     8461 gtcgggattg taaaactcaa aggaaaactc gctgtccaga tgtgcgggag cgatgcgttc
     8521 gagcttcgcc aacgccttca tgcggctttg agcttgaacg gctttagtgg ctttggcttt
     8581 gaagcggtcg ataaaggatt gcagatgttt gatttgcgcc tgctgtttga catatgcggc
     8641 ttgttgttgc gccagacgct gcgcgcgttc ggtttggtaa aaatcgtaat tgccgctgta
     8701 ttgcgtgagc ttttgctgcg acaattcaat ggtttgggtg gtggccgcat tgaggaaatc
     8761 acggtcgtgg gaaatgatga tttgcgtgca gggtaaagag gcaaggtggt tttccaacca
     8821 caagacagtt tccaaatcca aatggttggt cggttcgtca agcaagagca aatcagcgcg
     8881 gcaaatcagg gcttgcgcaa gattcaggcg catacgccag ccgccggaaa aggatttgac
     8941 gggacggctg tgttcttctt gcgaaaaacc cagcccgttc aacaattttg ccgcgcgggc
     9001 cggcgcggta taggcgtcga tttcttccaa tttggcatga tattccgcct gcttcatgcc
     9061 gtcattttgc acttccgcct gcctcaatgc cgtctgaaaa gcctgcaact cggcatcgcc
     9121 ctgcaaaacg taatccaaag cggaaatatc caaatcgggc gtttcttggg aaacggaagc
     9181 gagccgccag tttttcggaa tcgagatatc gccgccgtcc tgagtgattt cgcccttgat
     9241 taaggcaaac aggctggatt tgcccgtccc gttcttaccg atcaaaccga cgcgctgacc
     9301 gggattgacg gcagcggaag ccttgtcgag caggactttc aaaccgcgtt gcagggtgag
     9361 gtttttgatt tcaatcataa cggaaacatc gtcgggcggg aaaagcccgt attttacctg
     9421 aaagtcagtg ccgatgccgt ctgaaacggg aaatttacgg ctgaagccaa gcccaagccc
     9481 tgcgcccttc cgagtgcagg aaaaccaatg tcctgaatgc cgaatcggta ttcatgcatt
     9541 ccacgctgat tccgattcgg gaaaaatccg ccatgatttt gggatggata aactcctgag
     9601 ccgcgcccgt cccgataatc aatatttccg gatagtcaac aggtttgatg tcggacaaca
     9661 ggttttccgg agtcaggtcg gacaaggttc ggcattgcgg caggcagacc gaatccttat
     9721 gtacaagcac gggtttatgg aaactttgcc ccgccagccg gattccgccc gcgccgcatt
     9781 catattctgc aaactgtccg tctatcggat tttcttcaaa caacattttt taccccgttg
     9841 ctgcatcatc tgcaccgaaa ggggtgcaaa atcaggcgaa ttaatgtagg attggcagat
     9901 ttcatctgac gcgcctgccg atttcagacg gcatttgatt caaagtgcgg cacaattata
     9961 ccggcagcgg atattttcgt ctttcaatat ttacatttca gtcggcttac aaggagacac
    10021 aatgaagcca gtaaacatcg gtcttttagg cttaggtacg gtcggcggcg gtgcggctgc
    10081 cgtgttgcgg gacaacgcag aggaaatttc ccgccgcttg gggcgcgaaa tccgtatttc
    10141 tgccatgtgc gacttaagcg aagaaaaagc ccgccaaatc tgcccgtccg ccgcctttgt
    10201 caaagatccg ttcgaactgg tcgcgcgtaa agacgtcgat gtcgtcgtcg aattgttcgg
    10261 cggcacgggc attgccaaag aagcggtgtt gaaagccatt gaaaacggca aacacatcgt
    10321 taccgccaac aaaaaactgc tcgccgaata cggcaacgaa atcttcccgc tggcggaaaa
    10381 acaaaacgtc atcgtccaat ttgaagcggc agtagcgggc ggtatcccaa tcatcaaagc
    10441 cctgcgcgaa ggtttggcgg caaacaggat taaatccatc gccggcatta ttaacggcac
    10501 cagcaacttc atcctctccg aaatgcgcga aaaaggcagc gcgtttgccg acgtattgaa
    10561 agaagcgcag gcattgggtt atgccgaagc cgacccgacc ttcgacatcg aaggcaacga
    10621 cgcaggccat aaaatcacca tcatgagcgc gctggcattc ggtacgccga tgaacttctc
    10681 cgcctgctat ctcgaaggca tcagcaaact cgacagccgc gacatcaaat acgccgaaga
    10741 actcggctac cgcatcaaac tgttgggcat cacccgcaaa accggcaaag gcatcgaatt
    10801 gcgcgtccat ccgaccctga ttcccgaaag ccgcctcttg gcaaacgtcg acggcgtgat
    10861 gaacgccgtg cgcgtcaacg ccaatatggt cggcgaaacc ttatattacg gcgcgggcgc
    10921 gggcgcattg ccgaccgctt ccgccgtggt tgccgatatc atcgacatcg cccgcctggt
    10981 tgaagccgac accgcccacc gcgtaccgca tctggcgttc caacccgccc aagtccaagc
    11041 gcaaaccatc ctgcctatgg acgaaattac cagcagctac tacctgcgcg tccaagccaa
    11101 agacgaaccg ggcacgctgg ggcaaatcgc cgcgctgttg gcacaagaaa acgtgtccat
    11161 cgaagcctta atccaaaaag gcgttatcga tcagaccact gccgaaatcg tgattctgac
    11221 ccacagcacg gtcgaaaaac acatcaagtc ggcaatcgca gccatcgaag cactggattg
    11281 tgtggaaaaa ccgattacca tgatccgcat ggaaagcctg catgactgag ccgaaacacg
    11341 aaacgccgac ggaagagcag gttgccgcgc gcaaaaaagc aaaagccaaa atccgcacca
    11401 tccgcatttg ggcgtgggtc attttggcgt tgctcgcttc aaccgccctg ctctcccaat
    11461 gcgcgatgtc caaaccgcag gcaaaacaga aaattgtcga gtcttgcatg aaaaatattc
    11521 cgtttgctga aaaatggcag aacgatttga aagcgcgcgg cttggatgcg gacaataccc
    11581 gtctcgccgt cgactactgc aaatgtatgt gggagcagcc tttggacgga ttgagcgaga
    11641 aacagatcag ctccttcggc aaactcggtg cacaagaaca gcttgacctg ctcggcggcg
    11701 caaacgcgtt tgaaactcga gacaaacaat gtgtcgcgga tttgaaagcc gattgaacct
    11761 caggtctgga atgccgtctg aacgcgggga aaagcgtttc agatggcatt tttccatccg
    11821 ggtttgacgg gcagcccttc atccgaatcc gacgggcaaa cccctatcct gattcggcgg
    11881 ggaaaacaaa aagccgattc ttcaaggaat cggcttttgc tgaaaaacag tttacagtgc
    11941 gtctttcaat gctttacctg cacggaattt aagcgttttg gcggcggcaa tggtcagagg
    12001 ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt tcgccgacgt agaaagtacc
    12061 gaaaccgacc agagtaacgg tgtcgccttg tttcagggcg ttggttactg cgttggtagt
    12121 ggcatccaaa gctttttgtg cggcggcttt ggaaatatcg gcttcttgag caatcgcttc
    12181 gatcaattca gacttgttca caatcagtcc cttcctgtct taaaaaatga tgaaatgccc
    12241 gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt atagcaattc tgaaattgcc
    12301 gtgtcaagca aaaaatacgg aatcgcccta ttttacaggc tttcaggacg aaaccgcatt
    12361 tttacaacac atttcctgcg tttcaatgtt tgattgccct gctgcggggt ttcggttttg
    12421 aagcggattc cgccgccgct tccgcggcag aaggttctgc ccaagactca ggccggcttt
    12481 ccaaacccaa agccaatacc tcgtctatcc atttgaccgg atggatggtt aatccggttt
    12541 tcacgttttc agggatttct tccaggtctt tgacattgtc tttcggaatc aggacgtgtt
    12601 tgatgccgcc gcgaagcgca gccaacagtt tttccttcag accgccgatc ggcaggactt
    12661 cgccgcgcag ggtaatttcg cccgtcatgg caacatcggc acgcaccgga attttggtaa
    12721 aggcggacac catcgccaaa gtcatagcaa tgcctgcgct cggaccgtct ttcggcgttg
    12781 caccttcggg aacatggaca tgaatgtctt ttttctcgta aaaatcagga gccaaaccta
    12841 ctgattccgc acgggaacgg acaacagacc atgcagcgga tacagattcc ttcatcacat
    12901 cgcccaactg gccggtacac tggatcatgc ctttacccgg taatgctgcg gcttcgacag
    12961 tcagcaattc gccgccgact tccgtccaag ccaaaccggt aacctgtccg atgcggtttt
    13021 cactttcggc aacgccgtaa tcgaagcggc gcacgcccaa gtagtcatgt aggtttttct
    13081 cattgacttt aaccgcccta ggcttggctt tgctggtttt cttggtttca gacaacctct
    13141 tcttatcttc gttcaaggta atctgcatca ccaccttacg gcagattttg gcaatttcgc
    13201 ggtcgagcga acgcacgccc gcctctcggg tgtaataacg gataatatcg cgtaccgcgc
    13261 tttcttcgac caccaattcg ccttctttca cgccgttgcg tttcatttgc ttcggtacga
    13321 gatactgcat cgcgatattg attttctcgt cttcggtata gccggacaga cggatgattt
    13381 ccatacggtc aagcagcggg gtcgggatat tcaggctgtt tgaagtcgca ataaacatca
    13441 cgtcgctcaa atcgtaatcc acttcggcat aatgatcggc gaacttgttg ttttgttcgt
    13501 gatcgagcac ttcaagcaac gcgctggcgg gatcgccccg aaagtcgctg cccaatttgt
    13561 cgatttcgtc gagcaggaac aacgggtttc ttacgcctgc ttttgccata ttctgcaaaa
    13621 tcttgccggg catagaaccg atataggtac ggcggtgccc gcggatttcg ctttcgtcgc
    13681 gcacgccgcc caaggccata cggacatatt tccgccccgt tgctttggcg atggattcgc
    13741 ccaaagaggt tttgcccacg cccggagggc cgaccaaaca cagaatcgga cctttgagct
    13801 tgtccatacg tttttggacg gcgaggtatt ccaaaatccg ttctttgact ttttccaggc
    13861 cgtagtggtc ggcatccagc accagtccgg ctttggcgat gtctttgctg acgcgggatt
    13921 ttttcttcca cggcagtccg agcagggtgt cgatgtagtt gcgtacgacg gtggattcgg
    13981 cagacatcgg cggcatcatt ttgagttttt tcagttcgga caggcatttt tcttccgctt
    14041 ctttggtcat acccgccttt ttgatgcctg cctccaaggc atccagttcg ccgttttcgt
    14101 cttcttcgcc caattctttg tgtatcgctt taatctgttc gttcagataa tattcgcgtt
    14161 gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg tttttcggcc tgcataatgt
    14221 cgagttcgga ttccaacttt gccagcagga attccatccg tttgccgatt tcgggaatct
    14281 ccaaaatctg ttggcgttgc gccagtttca actgcaaatg cgctgcgacc gtatcggtta
    14341 gccggctgtt ttcggcaatg ccgttgatgc tgccgataat ttcggcaggg atttttttat
    14401 tgagtttggc gtattgttca aactgcgcca acagggtgcg gcgcacggct tcgaggtcgg
    14461 tattgccgcc cgtgtcttct tccacgaccg cctctatatg agaaacgaac agcccgcccg
    14521 tgtcttcgat ggtcaaaaca cgtccgcgat acagcccttc gaccagcact tttaccgtgc
    14581 cgtcgggcag tttcaacact tgcaggactt gtgcgacggt accggtctga tacaggtcgg
    14641 cggcaaccgg ttcttctacc gccgcatcgg tttgcgccaa caggaaaacc ggttcctcgc
    14701 gggtaatggc gttttccagt gcggcgatgg atttcggcct gccgacgaac agcggcagaa
    14761 ccatatgcgg gtaaacgacg acatcccgta aaggaagggt cgccaaagca gcatattcct
    14821 caaaatgctt ttctttttgt gtcatggata ctctcttgtg tctgacagga tacggattgc
    14881 cgcgtaaatt ggggttgaaa gtattatttc aagcatacgc ggtttattta tggagtttga
    14941 tgccatgccg tctgaaacat tccggcttca gacggcatgg gcttggaaag acaaggcggg
    15001 aacaaaaaaa cagttctgtg ttgccgctcc ttgctgtacc atccgtatgg tttgcggttc
    15061 tgccgcccta ttttcaaaac atgacgcagg tatcccatgt cttcttttat ttttcccgat
    15121 aacgggttcg aatggcgcaa cgaaagtctg caaaagccgc ccaaaggttg gtattatgtc
    15181 cgcgaaagcg gtgcagacgg cattttgaag gctacctatc aaaatattgc aactgtctgg
    15241 cagggcgatt tccacaatgc caaacaggtg ctttcggcaa tgaagaagaa ggtccgcaaa
    15301 cctgccgccg tccgttccga tgcggatatc gccgcccttt tccacgccca ccgtatgaag
    15361 caggcgcaac agagccgtat tctgaatatg cttgccgttg aaatccgccc cggtttcgtg
    15421 ttggacaaca aacgcgcgcc cgatatacgc gccgctttgc tcgacgtgta cggagaggcg
    15481 gacggcaaac cgttttttct gccgctcaat ctgctgctgg ggtttatggg tgtgcatgag
    15541 tggcataag
//
LOCUS       DAWTHD010000057        15198 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWTHD010000057 DAWTHD010000000
VERSION     DAWTHD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.057"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(323..4492)
                     /locus_tag="WM704_001302"
     CDS             complement(323..4492)
                     /locus_tag="WM704_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HFA9340721.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     AVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4547..6394)
                     /locus_tag="WM704_001303"
     CDS             complement(4547..6394)
                     /locus_tag="WM704_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HFA9340722.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6765..7994
                     /gene="sstT"
                     /locus_tag="WM704_001304"
     CDS             6765..7994
                     /gene="sstT"
                     /locus_tag="WM704_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFA9340723.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8145..9425
                     /locus_tag="WM704_001305"
     CDS             8145..9425
                     /locus_tag="WM704_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9340724.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPYP
                     SGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            9425..9628
                     /locus_tag="WM704_001306"
     CDS             9425..9628
                     /locus_tag="WM704_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFA9340725.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            9658..9936
                     /locus_tag="WM704_001307"
     CDS             9658..9936
                     /locus_tag="WM704_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340726.1"
                     /translation="MFRRPVFLTAVFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKG
                     IAFPNRGGATGCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(9929..10144)
                     /locus_tag="WM704_001308"
     CDS             complement(9929..10144)
                     /locus_tag="WM704_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340727.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(10137..10352)
                     /locus_tag="WM704_001309"
     CDS             complement(10137..10352)
                     /locus_tag="WM704_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFA9340728.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10555..11898)
                     /gene="argG"
                     /locus_tag="WM704_001310"
     CDS             complement(10555..11898)
                     /gene="argG"
                     /locus_tag="WM704_001310"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFA9340729.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            12023..12823
                     /locus_tag="WM704_001311"
     CDS             12023..12823
                     /locus_tag="WM704_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFA9340730.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12863..13849
                     /locus_tag="WM704_001312"
     CDS             12863..13849
                     /locus_tag="WM704_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFA9340731.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            14510..14761
                     /locus_tag="WM704_001313"
                     /pseudo
     CDS             14510..14761
                     /locus_tag="WM704_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14759..14952)
                     /locus_tag="WM704_001314"
                     /pseudo
     CDS             complement(14759..14952)
                     /locus_tag="WM704_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            15054..15198
                     /locus_tag="WM704_001315"
                     /pseudo
     CDS             15054..15198
                     /locus_tag="WM704_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3205 a         3883 c         4555 g         3555 t
ORIGIN      
        1 tttcttaacc atcccttcca atagccgtgc gaaggttttt ttatatcagt gggaaatgca
       61 atattctatt gtttttattg tagaatttta aaaacagatt gttgtgttcc gcgtttttgc
      121 cggtttggaa gcggtggggc gcatcagcct tttataaagg catatcggaa gcctgtataa
      181 ggtttttgaa catatcgatc ctgttccctt gcaggcggac ggcagcggcg gatggcgaaa
      241 ggcgggtttt caaatgtccg gtttggcggc gcgtctttca gacggcatgg cggcggagcg
      301 tgccgcgccg tctgaaaacc gtttatttcc ctttgccgtt tccggcggag tcttttttgt
      361 ccgaaccgaa gaggcggtcg aaacgtatgg tgtatgtcag ctcgccgccc gacgaacggc
      421 tgccgatacg ggcaaccgcc tgtatggcgc gggtcagccg gtaaatcagt ttgacggact
      481 gttccgcgct ggagatgccg tattcgtagc cgatgtagag tttgccggtc agttgtttgc
      541 cgacggtcag cacctgttcg gcggggttga gttcgccggt ttgcgcgttg cggctgcgct
      601 tgctggtaaa gcccaaatca tccaccagcc cgatgcggtc gttgatttgc ccggcaagca
      661 gcgcgcctgc ggctgcggac agggcggcat tgtcgccgct gctgccgctg ccggcgcggt
      721 tgaggatgag ccaggagagc ttgtcttttt cactcatcgg ttcgtttgcc gtcagcgtaa
      781 tgcgcgggct gttgaggctg cccaatattt ccacgcccgc accgacgggg gaaaggcggc
      841 gttcggcgcg gatgttgagg ttggggtcgt tgagcgggcc gacaaaggag actgtgcctt
      901 tggtaatgtc taaatcctgc ccgtatgctt tgtaacgccc tttgatgacg cggaccgtgc
      961 ccaccccacg cacatttccg cccggttgcg cggtcagggt cagtttgccg cctatggtaa
     1021 cgtccgcgcc gtagccggag aagcggatgc cgtcattgag gtctaaagtc aggttcatat
     1081 tgacggggag cgatgccgcc gcctctttct tgacttcgcc caatacgacg acatcgtcgc
     1141 cgacggacgg catcgaggat ttttgcgaac cgaacagccc ctgatcagtt ttaatcatac
     1201 cggtaacgga tatgcctttt tgcggcgaat agcgcaggcg ggtgttgccg gaaaccgtca
     1261 ggcggcggtt ggggcgggac aggatgcggt atttgtcgaa caccgcgccg atatcgacat
     1321 cgggcacgct gttttccatg ctgaccgtgc cggagagttc cgccgtccct tcgtgccgga
     1381 atttcaggct gtcgattacc catttcctgc ctgcaatatg cgaacgcagc gagccgttgt
     1441 ccaagatgat gccttgggtt tggttgcggt aatagagctt gtcgccgttg atactgccgc
     1501 ccaagtgcgg gtcggcgatg ctgccgccga gggttacggc ggcattcagg ctgcctttga
     1561 cggtttgtcc gaccggtagg aagttgcgga atacttcggc atcggcaacg gtcaggttga
     1621 gcctgccgcc caaaggtgcg gtatcgaagg agcggctttg cccgacggtg atattgccgt
     1681 tgattttccc gtagttgctg ctaccgttga cggcggcatt gacggacgga gagcttaccc
     1741 gtccgccgat ttgcgcggag gcattcaggc tgccggtaat gttttgcgcg gcggcgggca
     1801 gaaagggctt caatgcgccc aagtcgggaa gggaggctgt aatcctgccg ccgagcggtg
     1861 tatttgccat attgccgccg aaggcgttgc cgatgcccaa atcggcgtta atccgtccga
     1921 aacgcgcgcc gccgtcaagc aggattccga tgcggtcgtt ttgaaagcgc gttttcaggg
     1981 aaaatgcgtt caaacccaaa gcctgcccgc cgggcaatac ggcatcgccg ctttgccggc
     2041 tgatattgag gtagccgcgc gcgttgtgcc cgtaggcaac atcccagtcg ccgtttaaaa
     2101 ccagattgtg ttcgaagggc ggtttgaaga aattgtgcaa ctcggcgatg tgcaggccgc
     2161 gtgcgccgcc ttttgccgat atgccggttt tcctgtccca agaaaagtgt tgcaggttga
     2221 ggctgccgcc cattgcctgc caatttgccg cacttgccgc cacgcgttcc gcaccggctt
     2281 cgagcgtcat acggttttgc agcttgaggt tgaatgcgcc gccgatgtcg aggatgccga
     2341 tgctgccttt ccatcgggta agttccctgt tgatgccgcc tgaagcgtcc aaatcgagtt
     2401 tgaacggttt gccgtccagc gtcatggcgg cgtgtgtgcg gatgcggtgc tgcgcgcccg
     2461 taccttccag cgtcaggccg gcggtatcga caaccgccgc gccgcccgac agggaaaggc
     2521 ggccgccctt gatatcggcg cgcatcgggc ggcttgtgtc gggcgagcct ttgagggtaa
     2581 aatcgagcga acggatgtct gccgctttgc cgatgtgtaa gttgcgcgcc gtgccggaaa
     2641 ggtcggtttc aaaggttcgg atgccgccgt ccaaatcgcc ggaaaggtgt ccgcgtacat
     2701 ttaaagaccc cgcgagtccg aaaccgaaac gggataaatc gggtgcggtg atattgaggt
     2761 taagccggtc gccttttttg ccgaagccgc cgtctgtttt gacgatgttc cgccccaacc
     2821 gcaaatcgac ggcggcacgc ggaaggtggc gggactcgta aacaatgtcg gcactgccgg
     2881 caatcggcac gccgttgaac gtaccgggca aaaaacgcat tttgcccgta aatttctctt
     2941 ttgccagttc accggcaaga tgaatcgaac cgttgatatt gcctgccgga aattgcggat
     3001 cgatgcgtga agggtcgaat gcgcgggaac ggatgtccag cttgagcagg cggtctttaa
     3061 acagctcgag atagccttgc gcggtcaggc tgccttcccc ggcggagatg ttgacggtgt
     3121 cgaacaccag tttccgctgt tcgtttgcgg ggtcgcttgc gatggcgagg ctgccgtccg
     3181 tgcgtgccgt gccggtgccg agttgccaag agattttggg cgaggcggtc gtgccgccga
     3241 tgccgatgct gccgtccaac ctgcctttga acgcggtttg cagcacgtct tccgcgccga
     3301 cggagttgat gcctatattt aaatcaagga tgtctttttc ggtgtcgatt ttgcccgaca
     3361 gcctgatgcc gccccgtccg agcagggcgg cggacgtatt gccgatatgc accgtgccgt
     3421 cctgccggat gacaaagccg cccaaaacct gacggacggg gatgccgttg cggtcggcaa
     3481 agccggcttt ggtgttttcc aaatcgagcg agccttccag cgcgatgccg tctgaaaacg
     3541 acgggatggc ggtcaggtcg aaattcagcc cggcatcggg cagggaaggc acgaaggcgg
     3601 acggattgat gttgaatcct ttgaccagta cttcttccaa tgttttatcc aatgattcgg
     3661 caaacgggtg gatgacggat tttcccgaga ggcggatatt gccgccgtcg atcgtcagtt
     3721 cggcgcgcac atccttcagg ctgccgctca gccgcgccgt actgtgtatg gtttcgcctt
     3781 cgaatccgcc tttggtgtaa atggcggtat cgagggcaaa cggttttttc aagccgaccg
     3841 aggctgaccc cgacgaactg ctccacggcg tgtcggcggc cttcaggtcg aggcggtgcc
     3901 ctttacggtc gtaacggtat gccgcgttga ggcgttcgag atagacagtt tgtttgtcaa
     3961 aggttttgcc catgctgatt ttgcccgtct cgaagcggtc gagatagacg gcggcgggca
     4021 ggtctatgct gtcgggcagg ccttgaggcg ggcgttcttc tttaggcgga gtcggtttgg
     4081 ttacgatggc aatgtcgccg gcggagatgt cggtgatgtg caggctgcgg cgcatcagtt
     4141 cggacggttt ccacgcgaag cggaagcggc tgattttaag gtctgccccc tcggtttcta
     4201 tcgaccagtt gtcgccgtcg aagccgtcga gcagtgtgcc tttgaggttt tgggaggaaa
     4261 tgtttacgcc gaaccaggac gggatttggt acagcccgaa gcgcaaacct gcttccgtac
     4321 cggcgatcca gccgaggaaa catactgcca aaatcaggac agacagcagt gccgccgaca
     4381 gcttcagcag cgggcggcgt ttttttgccg gcggggcggg gcggtgttca gacggcattt
     4441 tgcgcgtgcc gttttcggtc ggatcggtat ctgtcggtgc ggtatcggtc ataatgtctg
     4501 tttcaaaatg gtttgctgca atgccgtctg aagcggccgt atcggtttag aaacgcgttc
     4561 ccaagctgat gtgccagcgg atttttttgt cgctgtgtcc gtaggcgatg tcgaaggaaa
     4621 acggcgcgag cgggctgaac cagcgcacgc ccagtcccga accgtgtttc agcttcatac
     4681 gtttgaaatt ggcggcggca tcgcccatat cgtggaatac cgcgccggac agggtgcgcg
     4741 taaacggcag ttggtattcc aagctgccca ccaagagggc gcgttcgggc aggaccgatc
     4801 cgttcggacc ggcaagcccg atgctgtcaa gttcgtaacc gcgcacggaa gacgcgccgc
     4861 cgctgcggaa catcagcccc gaggggacat cggcattgtc gcgtgcaacg gtgtaacccg
     4921 cttgtccgcg tatgatgaac gtgccgagtt ttttgttttc gggcgtgaag aaataacctg
     4981 cgcgggcaga ggtgcggatt agcgcggtgg aggacaggaa tgtgcccaaa gtcgtcccga
     5041 ttttgccgtc gaggtaatgg ccgttttcgg ggtgcagcac gttgttgagc agctggcgtt
     5101 tccaagaggc ggtcagcatc gtggcgtggc tgttgcccaa atcgacatcc gagccgggga
     5161 ttttccggcc ttctgcgaga aattccgccc ccagcctggc atcgatgccc gcgcggtcgc
     5221 gcacatacca gatgccgccg gagaaggcgc gtttttcaag gttttgggtg gtcgaacggt
     5281 tgtaggaaac gttgcttgtc cagtagttgc cctgatagtt gcgcggctgg ctgatgccgg
     5341 cggcaagcgt ggtttcgtat ttgtccatat cccagacgac cgagccgata tagcctttgt
     5401 tgaagaggtt gtaatagtcg taggcgattt tgccgcccaa accgtattcc gaatcgaggc
     5461 ggatgccggt ttcgagtttg tggcgtttga cctcggttac gctgactttg acggggacgc
     5521 ggtcgccttg gaggcggtcg aagtcggctt gtacggacgc gccggaataa tgcccgtttt
     5581 gttcgagcgc ctgttggaag tcgagcagca ggtcgaggtc gtagggcgtg cccggttgga
     5641 agcgcgccag gccggagacg gtttgttcgg ggtaacgctg tgtgccggtg atttcaaagt
     5701 cgccgaaggc gatggggcgg ccgctgtcca cgacgacgtt caaatcggcg gtggcggtat
     5761 cggggttgac ggccgcccgg gtgttgccga gcttggcaag cgggtagcct ttgcgcgtta
     5821 ccgcgccgag gacggaagtt ttgctgtttt cccaactgtc ctgatcgaaa tcgctgccta
     5881 ccggctgctg ccagttttcc agcgcgttgc ggtagtattc ggcgaggttg ccgtctgaaa
     5941 ggatgtcgcc gaggatggcg acgccgacgt tggcgatttt ggtgcgcggg cccggtgtga
     6001 tgtgcaccgt ataagctccg tctttttccg tcaggctgac cttgctgctg aaatagcctt
     6061 tgctgcggag catggttttg acgttgtccg gtgcttcttc ggcaaggaat cccgtctgtt
     6121 ccttatccaa aacctcttcc tgctgctgcg tgatgagcgg caggtgttct tcgaccatat
     6181 ctttgatttc actgtcctgc gtgtcgatgc ggacggggaa tttgggtttt aatttgactg
     6241 attcggtgtc ggggcttttg cttttgaaca atgcgaaacc cgccgccttg ttttcggaaa
     6301 ggtcggcggc aggcgcgtat gcgtgcggaa agaaaaataa agccggcagg agcagggcgg
     6361 tcggtttgat catcatggta cgggtgtcgt gcatcggtgt ggcggatttc gattctaaca
     6421 ctgttcggac ggggcgggac gcttcagacg gcatactgcc gacggtattt cggaggttgc
     6481 ggcccgccgg aaatgtatct gcttgtttta aggcattttg tttccaatcc gcatatggaa
     6541 aacaggctgc gtttcggaat agtgtcgaaa aataggcagg attaaataaa tttattctcg
     6601 ttcaataaat taaaccgttg cgccgaatga taaaacaaag cctgtccctg catagggcaa
     6661 atcaacgcac ctcaaaacac agcttgaaaa ataaaaaaac acagagaatg agttttcttt
     6721 taaaatacaa cattttttaa catatcacaa cataaggaag aattatggcg ttcggcaaat
     6781 ccttatttca tgcgatcggc cgcgtcagcc tggtcaggca gattgccgcc ggtttggcgt
     6841 tgggcatcgt aatcggttcg gtttcaccgc aactgggctt ggcggcaggc ttgttcggca
     6901 gcctgtttgt cggcgcgctc aaagcggtcg cgccggtttt ggtatttatt ttggtggcgg
     6961 ccacaatcgc gcagcaccaa aaaggcaaca aggcgcatat caggccgatt atcgtccttt
     7021 acctcatcgg cacgttttcc gcagccctga ccgccgtcat cgccggtatg gttttcccga
     7081 cgcacattgt tttggcgggc gcgggcgatg tgtccgccgc gccgccttcc ggcattgtgg
     7141 aagtgttgaa atcgctgctg atgaacctgg tcgccaaccc gattaacgcg attgccaatg
     7201 ccaactatat cggcattttg gcttgggctt tggttttggg cgcggcgttg cggaatcacg
     7261 gttcggacgt tacgcggcag gtcgttgccg atttggcgga agcggtttcc accgtcgtga
     7321 aatggattat ccgttttgcc cctttgggta ttttcgggct ggtgtcgtcc acaatcgcgg
     7381 agacgggttt cggcgcgctg gcgggttatg cgaagctgct tgcggtgctg ttgggctgta
     7441 tggcgtttat cgcgctggcg gtcaatcccg ccatcgtgtg gtggaaaatc cgccgcaacc
     7501 cttatccgct ggtgtttacc tgcctgcgcg aaagcggcgt gtatgccttc tttacccgtt
     7561 cttccgccgc caatatcccc gtgaatatgg ctttggcgaa aaagctgggg ctgcacgaag
     7621 acacttattc gatttccatc ccgttggggg cgaccgtcaa tatgggcggc gcggcgatta
     7681 cgattaccgt tttggctatg gcggcggcgc acacccaagg catacaggtt gattttgcca
     7741 ccgcgctgct gttgagcctg gtggcgacgg tcagcgcgtg cggcgcgtcc ggcgtggccg
     7801 gcggttcgct gctgctgatt ccgctggcgt gcagcctgtt cggcatcagc aacgatgtcg
     7861 ccatgcaggt ggtcgccgtc ggcttcatca tcggcgtgat tcaggattcg gcggaaacgg
     7921 cgttgaattc ttcaaccgac gttttgttta ccgctgccgc cgatttgggc cgacagagaa
     7981 accgtgcgga ataaaccaag ctatgccgtc tgaagcccgt ttggcgttca gacggcatat
     8041 ttttacaatt ttatgggcat atggcggaaa caataccggc tgccttgaat atgtttcaac
     8101 acacagggcg atacataaag cgtcgcccta tgaaggagat gatgatgttt aaacgcagtg
     8161 tgattgcaat ggcttgtatt tttccccttt cagcctgtgg gggcggcggt ggcggatcgc
     8221 ccgatgtcaa gtcggcggac acgccgtcaa aaccggccgc ccccgttgtt gctgaaaatg
     8281 ccggggaagg ggtgctgccg aaagaaaaga aagatgagga ggcagcgggc ggtgcgccgc
     8341 aagccgatac gcaggacgca accgccggag aaggcagcca agatatggcg gcagtttcgg
     8401 cagaaaatac aggcaatggc ggtgcggcaa caacggacaa ccccaaaaat gaagacgcgg
     8461 gggcgcaaaa tgatatgccg caaaatgccg ccgaatccgc aaatcaaaca gggaacaacc
     8521 aacccgccgg ttcttcagat tccgcccccg cgtcaaaccc tgcccctgcg aatggcggta
     8581 gcgattttgg aaggacgaac gtgggcaatt ctgttgtgat tgacggaccg tcgcaaaata
     8641 taacgttgac ccactgtaaa ggcgatcctt gtaatggtga taatttattg gatgaagaag
     8701 caccgccaaa atcagaattt gaatcgttaa gtgatgaaga gaaaattaag aaatataaaa
     8761 aagatggaga aaagtttact ggtttggttg ctataaaagt tgaaaataat gggttgaata
     8821 aatataccat tatttatcaa gctcagccta ctcgttctgc acggtcgagg aggtcgcttc
     8881 cggccgagat tccgctgatt cccgtcaatc aggccgatac gctgattgtg gatggggaag
     8941 cggtcagcct gacggggcat tccggcaata tcttcgcgcc cgaagggaat taccggtatc
     9001 tgacttacgg ggcggaaaaa ttgcccggcg gatcgtatgc cctccgtgtg caaggcgaac
     9061 cggcaaaagg cgaaatgctt gcgggcacgg ccgtgtacaa cggcgaagtg ctgcatttcc
     9121 atatggaaaa cggccgtccg tacccgtccg gaggcaggtt tgccgcaaaa gtcgatttcg
     9181 gcagcaaatc tgtggacggc attatcgaca gcggcgatga tttgcatatg ggtacgcaaa
     9241 aattcaaagc cgccatcgat ggaaacggct ttaaggggac ttggacggaa aatggcggcg
     9301 gggatgtttc cggaaggttt tacggcccgg ccggcgagga agtggcggga aaatacagct
     9361 atcgcccgac agatgctgaa aagggcggat tcggcgtgtt tgccggcaaa aaagatcggg
     9421 attgatgttg atgccgcccg aagcgcaggt attcattacg gcgggacgac gcgttcaaat
     9481 catcaaacaa gcccccgaaa aaggcggcat cccaaaatcc ggcactgaaa ttgccgcgcg
     9541 tatcggcggc attccgcagc ctgccgggga aaccgtcggc agcagccgga atgtcgggta
     9601 tttccgcccg cctttttcgg gctgttgaaa tatgaaaata aagcataggc cgtctgaatg
     9661 ttcagacggc ctgttttttt aacggctgtg tttcaacact ccgccggttc caaccggcag
     9721 cagcagggag ataatgcctt gaaccccgct cttcggggcg gcagggttcg ggccggtttg
     9781 gggaaaggca tcgcctttcc gaatcggggc ggtgcaacgg ggtgcgcctt atgtgccgaa
     9841 agggtttgcg gcaacggtat gccgtctgaa gcgggtgcgt tcagacggca ttgtgatgtt
     9901 tggggcgcgg gtccggttca aatccgcatc agttgataaa cccgttgtct ttcattttct
     9961 tttcagaaaa atactggccg cataccagca gggcggcgag gatgatgaca aacagccctg
    10021 ccatagtcag tgtgattttg aagaacaatt cggcttcgag aaaagaaaac caaagttgca
    10081 caatcagcag cagcacccag ccggcaaaca gtccgagaca ggcataaacg ccgagcttaa
    10141 acacggtccc gctccaaaat gacgacgtag gcttcgcgtt tgaaaaacag cagcatacgg
    10201 cgcaaatctt tttccatcgt tacgacccgc cagccttcgg gggtttgttt gttgaggaac
    10261 gcgctgaaat tgacggggtt gacctttgcc gcgccgaaaa acaggctgga caacaggctt
    10321 tcctgataaa tgacgacttt gtattctttc atcgtatttc cttttcggtt gaaaccccgc
    10381 cctttggggc ggtaggatca gactttattt gggatggaac cccttccaaa tcaggacgat
    10441 acatagggcg gtgctttatg tatcgtcctg tgtgttgaaa cataggattc ctttaatcaa
    10501 gggattgcct tttcaggcaa tcccttaaat tgcagcgatg caatgcggcg aaatttattg
    10561 cttattgccc aactgcggca ataccgagcc ttcgcccagc gagagcaaac cgctttgcga
    10621 ataaataccc agtttggcgc gggtgtcggt gatgtcgagg tttcgcatcg tgagctgtcc
    10681 gatgcggtcg agcggtgtga acgccgcacc ttcgactttt tccatactca ggcgttcggg
    10741 ttggtaggtc aggttgggcg attcggtgtt cagaatcgag tagtcgttgc cgcgccgcag
    10801 ttcgagggta acttcgccgg taacggcttt ggcgacccag cgttgggcgg tttcgcgcaa
    10861 catcaaggct tggctgtcga accagcggcc ttggtagagc aaacgtccga ggcgcaggcc
    10921 gttgatgcgg tattgttcga tggtgtcttc gttgtggatg ccggtaacca agcgttcgta
    10981 ggcgatgtgg aacaatgcca tgcccggggc ttcgtagatg ccgcgcgatt tggcttcgat
    11041 gatgcggttt tcgatttggt cgctcatacc caagccgtgg cggccgccga tgcggttggc
    11101 ttcgaggaag agttcgacgg ggtcggcgta ttctttgccg ttcaatgcaa ccggcacgcc
    11161 ttcttcaaag cgcacgctga cttcttcggg ttcaatttcg acgttttcgt cccagaaggc
    11221 aacgcccatg atgggcttaa cgattttgat gcccgagttc aaaaattcca agtctttggc
    11281 ttcgtgggtc gcgccgagca tattggaatc ggtggagtag gctttttcaa ccgacatttt
    11341 gtagttgaag ccgttggcaa tcagaaattc gctcatttcg tgacggccgc cgagttcgtc
    11401 gataaattgc tggtcgagcc agggtttgta gattttcagc gcgggattgg tgagcaagcc
    11461 gtagcggtag aaacgctcga tgtcgttgcc tttgtaggtg ctgccgtcgc cccagatatt
    11521 cacatcgtct tctttcatgg cggaaacgag catggtgccg gtcacggcgc ggcccagagg
    11581 cgtggtgttg aaataggcga tgccgccggt ggaaacgtgg aacgcgccgc attggatggc
    11641 ggcgatgcct tcgtgtgcca gttgtgcgcg gcagtcgatc aggcgggcgt tttccgcacc
    11701 gtattccatt gcttttttgg gaatggcgtt gtaatcgtct tcgtcgggct ggccaaggtt
    11761 ggcagtgtag gcataaggca gcgcgccttt gagtttcatc cacaacagcg cggcagaggt
    11821 atcaagaccg ccggagaagg cgatgccgac tttctgaccg acggggaggt tttgtaaaat
    11881 agtatggttt tggttcatga aaaaatcctc gatagcttga aaaatcaaag ccattatctt
    11941 ataaatagaa aatattgtca gccatcatgc cgtctgaacg ggtattccgc cgtttcaggc
    12001 ggtatttaaa accgaaaaca ttatgaacgc gttcaacctc atcatcatcg gagacgaaat
    12061 cttacacggc agccgccaag acaagcattt cgcctttttt aaatccctgc tcgaatccaa
    12121 agggctgaag ctcaatcagg tgcaatatct gcccgacgaa cccgatttgc tggtcaggca
    12181 actgcgccgc agcttttcag acggcatacc gacgttcgtt accggcggca tcggcgccac
    12241 gcccgacgac cgcacgcgcc aaaccgcagc ggctgctttg gatttgcccg tcgtccgcca
    12301 tcctgaagcc gccaagttta tcgaaggcat aacccggaaa cgcggcaagc cgctcgattc
    12361 gcccgaacac gcccaacgcc tgaaaatggc ggatttcccc gagggcgcag agcttgtgcc
    12421 caacccgttc aacaacatcg ccggattttc catccgcgag cattatttct tccccggctt
    12481 tcctgtgatg gcgcacccga tggcggaatg ggttttggaa acttattatg ccggccgctt
    12541 caaccaaacc gaacgcggca gccgcagcgt gtatgtgttt gagcagcccg aatcgcgcat
    12601 tacgccgatt atagagcata tcgagcaaac ttatcccggc gtgcgttcgt acagcctgcc
    12661 cagcgtcggt tggacgcatt cagacggcac gcaggtcaaa ccgcacattg agttcggcat
    12721 caaagccgaa ggagaagccg tcaacctttt ggacgcggcg tgggcggaag tgttgcacag
    12781 cttggacgga ttgggagcag agctgaaaaa tcgggtaaac tgaaaactgt ttgcccaaat
    12841 cggacgatat ttaaggaaaa ccatgcaata ccgaatcaga agagaaaacg aagcacccga
    12901 agcaaaaaat gcgggggaaa ccttatggga gcgcgacatt atgcgcgaag tgctgctgtc
    12961 cgcctatcgg gacaggcgca gggagcggat gtggaaaaac atctggcgcg ccgtcagcac
    13021 cctgattctg gttgccctga ttgcaggcat tttccgaaaa gacgaagccg cattgcagtt
    13081 ggcgggcaac acgccgcata ccgcagtcgt caacctgtac ggcaaaatcg gcaacggcgt
    13141 agaagaccag gtcaaaaaac tcaaagacgg gatggaggcc gcctacaaaa acccgcaggc
    13201 aaaagccatc gtcatccgcg ccaacagccc cggcggttcg cccgtcgtct ccaacaccgc
    13261 ttttgaagaa atacgccgtc tgaaggcgca gcaccccggc atccccgttt atctcgtggc
    13321 agaggatatg tgcgcgtccg gctgctacta catcgcggcg gcggcggaca aaatctatgc
    13381 cgacccgtcc agcatcgtcg gcagcatcgg cgttatcggc agcagtttcg acgcgaccgg
    13441 cctgatggaa aaaatcggcg taaaacgcag ggttaaaatt gcgggcagca acaaaggcat
    13501 gggcgatccg ttttcgcccg aaacgcccga acagtcgaaa atctgggaag aaatgctgac
    13561 cggcatacac ggcgaattta tcaaagccgt caaaaccgga cgcggcggcc gcctcaaatt
    13621 ccgacaatat cccgacgtgt tcagcggtcg cgtgtacaca ggcgcggacg cgctgaaagt
    13681 cgggctggtg gacggactcg gcaatattta cagcgtggcg cgagacgtgg tcaaagctcc
    13741 cgatgtggtg gattacactc cgaaggacga tttcggcaga atcttgggca gacgcttcgg
    13801 agcagaactc aaagcaagcg tttcggaagc tctgcaggcc gtccggtagc gttgtttaca
    13861 gtatttctaa gggaatacca gaataaaaat atacacaaaa acactcacct ccccagtatg
    13921 ctgccactaa gtcagccgcg ttactattta ccgtgcactt aaagccgcaa aaggcaggtt
    13981 gtccccggga ggttttgtgc atcctgatac cgatcggttg ctgttgctgt tgctgttgct
    14041 caaaggacgg aaggccgccg ataaacgaga ttacctgttt gtcgctattg acgattttta
    14101 tactctgcca ttttgccgga caaaaccgca gacagtgctg ccaagtttct gaccgaacat
    14161 ctggctgacc cctgcttgta cctggttgag tacgtttact ctgacaatga taggtaatat
    14221 aaagagccgt ccaacatgct ttcggtgcag cttgttatga taatgggatt ggttggaggc
    14281 ttgcccgatt tgcttgtccg cagaccaacg gtaaggcgga gcgggttatc cgtaccttga
    14341 tggagatgtg gcatgaggaa cagtcgtttg acagtccgga acatcagcaa aaggagttgt
    14401 gctgctttat taatttaggc gctgtcctag ataactaggg aaattcaaat taagttagaa
    14461 ttatccctat gagaaaagcc gtctaagccg gtataaacaa aataaactca ttgggctatt
    14521 tgtcgcaggt gtaactgcaa gaacagcggc agagttggta ggcgttaata aaaatactgc
    14581 agcctatgat tttcatcgtt tacgatgact gatttatcaa aacggtccgc atttggaaat
    14641 gtttgatggc gaagtagaag cagatgaaag ttattttggc ggacaacgca aaggtaaatg
    14701 cggtcgcggt gctgccggaa aagtcgcagt attcaggctt ctcaaacgaa atggcaaggt
    14761 ttcctttgcc gtccgttatg tacgtttcgt aacattcgaa gaattcctga accctgcccg
    14821 ccgtttcggt ttccgtaccc ccctgttaga taagggggga agattcgaag cggttgccgg
    14881 cttcctgccg tccgccggcg cgtccgtcat cacgccggca accgcctttg tcatcccttg
    14941 cttatctccc atgtgcgaat cctcaaaaac gggcaaaaaa aaagccctgt tacttgtaga
    15001 aagtaaaggg cgttaatttt tgttaatcgc cccttcttag ggacgcaata tataaggttt
    15061 atacggttac tgtagcgaat actcaaaccg ctactttatt tcctattatc cgcgaacaag
    15121 tgaaacctga cagcattgtt tatacggatt gttatcgtag ctatgatgta ttggatgcag
    15181 gcgaatttag ccatttcc
//
LOCUS       DAWTHD010000058        14827 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWTHD010000058 DAWTHD010000000
VERSION     DAWTHD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14827
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.058"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            201..1724
                     /locus_tag="WM704_001316"
     CDS             201..1724
                     /locus_tag="WM704_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9340732.1"
                     /translation="MYRVCRLCAALCVWGAVGAYAAGLPDVRDDAAALRAQRAAAEGW
                     AGMPPEGDSAANGGSRVIDGDFLLSRPQLLEHVLRDALNGNQADLIASLADLYAKLPD
                     YDAVLYGRARALLAKLAGRPAEAVARYRALHRDNAADERVLLDLAAAEFDDFRLKSAG
                     LHFAEAAKLDLPAPVLENVGRFRKKTEGLTGWHFSGGISPSVNKNANNAAPQYCLQGG
                     GSRICSVTGPVRAAGLDYEAGAEKLTALADNHYLLFRANVGGTSYYFSRKSAYDDAFG
                     RAYLGWQYKNARQTVGVLPFYQAQLSGSDGFDAKTKPPADRRLAPYMLAHGAGVQLAH
                     SYRLSRRSQLSVSLERYRQRYREQGRAERNDGWQDGLYVSLARRLGGSATVFGGWQFA
                     RFVPKRETVGGAVNNAAYRRNAVHIGWAQEWGGTGGLNSRIAASYAHRNYKGIAAFST
                     EAQRNREWNVSLALSHDKLSYKGIVPTLNYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1785..3659)
                     /locus_tag="WM704_001317"
     CDS             complement(1785..3659)
                     /locus_tag="WM704_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HFA9340733.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            3830..4759
                     /gene="ppk2"
                     /locus_tag="WM704_001318"
     CDS             3830..4759
                     /gene="ppk2"
                     /locus_tag="WM704_001318"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFA9340734.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMRRAVYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(4841..6145)
                     /gene="aldA"
                     /locus_tag="WM704_001319"
     CDS             complement(4841..6145)
                     /gene="aldA"
                     /locus_tag="WM704_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFA9340735.1"
                     /translation="MTAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAE
                     GGKTKDLARVEVMFTADYLDYQAEWARRYEGEIIQSDRPRENILSFKRPLGVIAGILP
                     RNFPFFLIARKMGPDLVTGNTIVVKPSSVTPINCHIFAEIIDAVGLPAGVFNVVDGPG
                     AEIGNALSAHPQVDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLD
                     LAVKSILASRVGNTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGAP
                     EMGTLIEERAVKAVAEKVERAVKQGAKLVCGGKRTEGRGYFFEPTLLTDTDNSMDIMK
                     EETFGLVLPVSAFDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINR
                     ENFEAMQGFHAGWKKSGIGGADGKHGLEEYLQTQVIYLETDI"
     gene            complement(6072..6272)
                     /locus_tag="WM704_001320"
     CDS             complement(6072..6272)
                     /locus_tag="WM704_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFA9340736.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGRADVD
                     RGARGATGLGASACGRTRRVFA"
     gene            complement(6380..7228)
                     /locus_tag="WM704_001321"
                     /pseudo
     CDS             complement(6380..7228)
                     /locus_tag="WM704_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221212.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7468..8268
                     /locus_tag="WM704_001322"
     CDS             7468..8268
                     /locus_tag="WM704_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9340737.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8316..9092
                     /gene="mlaE"
                     /locus_tag="WM704_001323"
     CDS             8316..9092
                     /gene="mlaE"
                     /locus_tag="WM704_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFA9340738.1"
                     /translation="MNFIRSVGAKTLGLIQSFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9143..9637
                     /gene="mlaD"
                     /locus_tag="WM704_001324"
     CDS             9143..9637
                     /gene="mlaD"
                     /locus_tag="WM704_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFA9340739.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9668..10258
                     /locus_tag="WM704_001325"
     CDS             9668..10258
                     /locus_tag="WM704_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFA9340740.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGTSLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10309..10587
                     /locus_tag="WM704_001326"
     CDS             10309..10587
                     /locus_tag="WM704_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFA9340741.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10584..11411
                     /locus_tag="WM704_001327"
     CDS             10584..11411
                     /locus_tag="WM704_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFA9340742.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11536..11898
                     /locus_tag="WM704_001328"
     CDS             11536..11898
                     /locus_tag="WM704_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFA9340743.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            11895..12281
                     /locus_tag="WM704_001329"
     CDS             11895..12281
                     /locus_tag="WM704_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFA9340744.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            12278..12766
                     /locus_tag="WM704_001330"
     CDS             12278..12766
                     /locus_tag="WM704_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFA9340745.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(12847..13014)
                     /locus_tag="WM704_001331"
     CDS             complement(12847..13014)
                     /locus_tag="WM704_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFA9340746.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVITKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(12981..13343)
                     /locus_tag="WM704_001332"
     CDS             complement(12981..13343)
                     /locus_tag="WM704_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFA9340747.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLLYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
     gene            13353..13480
                     /locus_tag="WM704_001333"
                     /pseudo
     CDS             13353..13480
                     /locus_tag="WM704_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            13582..13797
                     /gene="rpmE"
                     /locus_tag="WM704_001334"
     CDS             13582..13797
                     /gene="rpmE"
                     /locus_tag="WM704_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFA9340748.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            14054..14689
                     /locus_tag="WM704_001335"
     CDS             14054..14689
                     /locus_tag="WM704_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFA9340749.1"
                     /translation="MFYDSKCCYFNNPVFWDSRRLTYYPNVIFCQKKSGKDIINIYLG
                     QFLGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGESIAKEGL
                     RKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQ
                     KLAQVPSVGETLEKYSRWFVAVVYLGLGIYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         3251 a         4033 c         4131 g         3412 t
ORIGIN      
        1 ttttatagcg gattaacaaa aaccggtacg gcgttgcctc gccttgccgt actatctgta
       61 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatg atgcgccgcg
      121 tccgccgcgc gtggggcttg cggcgtttct gccgccgccg tccggcggtg ttttttaagg
      181 aaggcaaatt gaaggatagt gtgtatcggg tatgccggct gtgtgccgcg ctgtgtgttt
      241 ggggtgcggt cggcgcgtat gccgccggct tgcccgacgt tcgggacgat gcggcggcgt
      301 tgcgcgccca gcgtgcggct gcggagggtt gggcgggaat gccgccggaa ggggattctg
      361 ccgcaaacgg cggttcgcgc gtaatcgacg gggatttcct gctgtcgcgc ccgcagttgt
      421 tggaacacgt tttgcgcgac gcgctcaacg gcaatcaggc ggatttgatc gcttcgttgg
      481 cggatttgta tgccaagctg ccggattatg atgcggtgtt gtacggcagg gcgcgggctt
      541 tgctggcgaa attggcggga aggccggcgg aggcggtggc gcgctatcgg gcgttgcaca
      601 gggataatgc ggcagacgag cgggttttgc tggatttggc ggcggcggag tttgacgatt
      661 tccggctgaa gtcggcaggg ctgcattttg cggaggcggc aaaattggat ttgccggcgc
      721 cggttttgga aaatgtgggg cgtttccgga aaaaaacgga ggggctgacg ggctggcatt
      781 tttcgggcgg catcagccct tctgtgaaca aaaacgccaa caatgccgcg ccgcaatact
      841 gcctgcaggg cggcggcagc cggatatgca gcgttaccgg acctgtccgt gcggcagggt
      901 tggattatga ggccggggcg gaaaagctga ctgcgctggc ggacaaccat tatctgctgt
      961 tccgcgccaa tgtcgggggc acaagctatt atttcagccg caaatcggct tatgacgatg
     1021 ctttcggcag ggcttatttg ggctggcagt ataaaaatgc gcggcagacg gtgggggttt
     1081 tgccgtttta tcaggcgcaa ttgtcgggta gcgacggctt tgatgcgaaa acgaaaccgc
     1141 ctgccgaccg ccggcttgcg ccgtatatgc tggcgcacgg ggcgggtgtg cagttggcgc
     1201 attcttaccg cctgagccgc cggtcgcagc tttcggtgtc gctggaacgt taccgccaac
     1261 gctaccgcga acaaggccgg gcggaacgca acgacggttg gcaggacggg ctttatgtgt
     1321 cgctggcaag gcggttgggc ggctcggcaa ccgtgttcgg cggctggcag tttgcgcggt
     1381 ttgtgccgaa acgcgaaacg gtgggcggcg cggtcaacaa tgccgcctac cgccgcaacg
     1441 ccgtccatat cggttgggcg caggagtggg gcgggacggg cggtttgaac agccggattg
     1501 ccgcgtctta cgcccaccgc aactacaaag gcattgcggc attttcgacg gaggcgcagc
     1561 gtaaccgcga atggaatgtc tcgctggctt tgagccacga caagttgtcg tacaaaggca
     1621 tcgtgccgac gttgaactac cgcttcggta agacggaaag caatgtgccg tatgcaaaac
     1681 gccgcaacag cgaggtgttt gtgtcggcgg attggcggtt ttgaatggtg ggataatgcc
     1741 gtccgaactt tgcggcagca ggttcggacg gcatttttgc gcgttcaggc aaggacggcg
     1801 gcaaatacgc cgcgcaaggc gttggagagg cggatttctt cggctccttg cagtgttttt
     1861 tgtgtgatgt gtgtttcaat cacttgattt gtgtgcaaat atttttgcgg ttcgtccgac
     1921 acggcttggc gcattatgcc gtttaaaatg tctaaatcta aagagggcgt gagccattgt
     1981 ccgcgatgtt tgacgaacac gttgctcctg ccgccttcga gtaggatgcc gtccgaattg
     2041 aaaaacaggc tgtcgaacgc gccttgcgtt tcggcggttt gccacgcttg gtcgaagatg
     2101 gcgcggtgtg tggttttgaa gcggcgcagg tagttttgtg cgggcaggat ggtcggcgaa
     2161 atgatgacgc gctgtttgtc ggtcagacgg tttaaaacgg cgtgggacag gctgatgccg
     2221 tctgaagcga gcagggcttt gatgcggaac gcgccgtcgg gcaagtcggc aatgtattgt
     2281 ttgatttgat tttcgcagcc gtcgggcagg ggcaggttga gggcttgggc ggaggttttc
     2341 agacggcata ggtggcggtc gagcagggcg cattgcctgt tttccgcgcg cagggtttca
     2401 aaaatgccga aatcggggcg cagttcgttg aggaatcggg ctttccagcc gcattcgcga
     2461 tattcggcgg cggggtcgct gtcgatgacg atgccggaac cgacaccgta cacgccccga
     2521 tacggcggat tggcttcaaa cggatgcggc gtttccccgc cttgtcctgc cgttcgggct
     2581 tgcgcgtttg aatccgtgcc gccgatgcct gaaacaatgc cgtctgaaac gggtttgagc
     2641 gacagggtgc ggatgacgac gttgaacgcg ccttcaaacc ccaagccgcc ggaacacggg
     2701 ttcaaatagc cgatgctgcc cgtataaagc ccgcgcgctt cggtttcgag cgattcgata
     2761 atctgcatac tcattttttt gggcgcgccg gtgatgctgc cgcaggggaa ggcggcgcgg
     2821 agggtgtcgg cgaacgaggt gtccggcagg gcttgcgccc ggatggtgct ggtcatctgc
     2881 cagacgctgc cgaaacgcga tactttaaac ggctcgggta cgcatacttt accggtttgg
     2941 gcgattttgc cgaggtcgtt gcgcagcaaa tcgacaatca tcacgttttc ggcgcggttt
     3001 ttcgggtcgg tttgtaactc ggcggcgcgg cgttcgtctt gtccgtcgcc caaaatcggc
     3061 gcggtgcctt tcatcggttc ggtgctgatg gtgccgtctg aagcgatgtt gaggaagagt
     3121 tcgggcgaga aacacagcgt ccacgcggat tgcccggctt catcgggcag gtgggacaat
     3181 acggcatagg ggacgggctg gcgcaggcgg cggtagaggc tgacggggtt gccgtaggct
     3241 tgcaggtgca ggcgggtggt gtagttgatt tgataggtgt cgccgcgccg gatggcttcg
     3301 tggatttggc ggatgcggtc gaggtaatcg gtttcggata cggaggattg cggcgtggaa
     3361 ataccggcgg ggatgccgtc tgaatgtcgg gcaagccagc tttcggcatc ggtgtcggcg
     3421 cagtcggcaa accagtgcag ggcaatgttg ccgccgcgtt cggattccat ccccatcagc
     3481 ggcaaaccga attcgtagtc tgcaaacaac accgaatgca gccctttttg ccatcccgat
     3541 tgcagcgcgc cgtctaaagc atcgagttct tcgggacgga aaaaacggct ttccacatga
     3601 ttttgataga gttttgcgcg tcggctgacg gcatcgtcaa acagggcgaa ataaggcatg
     3661 gcaattcggg gcaaatgttt tgattatacg ccccttttac acggattttc agacggcggg
     3721 gcaaattcgg gcaacaaaat gtaattttat gtagagaaag cggtggcgaa agtgtaaaat
     3781 tgtgaaaacg gactttcatt tccaacctga aatacaatag gagaccttta tgacagaccg
     3841 ccagttgcaa ccgtttgaaa acgtagaatt gggcgaaaag caagaccagc tccaagtatt
     3901 tgaaaaagct gttttggaac atgaaggcaa aggttctgcc gaagattccg gcacagcccc
     3961 gctgcccgaa aactacccct gccgcaaacg tatgcgccgt gccgtatacg aagccgaaaa
     4021 agccaaactg caaatcgagc tgctgaaagt gcaaagccgg gtcaaagact ccggccagcg
     4081 catcgtcagc ctgtttgaag gccgggatgc ggcaggtaag ggcggcacca tcaaacgctt
     4141 tatggaacat ttaaacccgc gtggcgcgcg cgtggttgct ttggagaaac caaccactac
     4201 cgaacgcgga caatggtatt tccaacgcta catccaaaac ctgccgactg cgggcgaaat
     4261 ggtattcttc gaccgctcgt ggtacaaccg cgccggcgtg gaacgcgtga cgggcttctg
     4321 cgaacccaac gaatatatgc tctttatgtg ccaagcccct gagttggaac gtatgcttgt
     4381 cgccagcggc atccacctgt tcaaattctg gttctccgta tcccgcgaag aacaactgcg
     4441 ccgcttcatc tcccgccgcg acgacgccct gaaacactgg aaactttccc ccgtggacat
     4501 ccagtcgctc gaccgctggg acgactacac cgaagccaaa aacgcgatgt tcttccacac
     4561 ccacaccggc gacgcgcctt gggtcatcat ccgttcggac gacaaaaaac gcgcccgttt
     4621 gaactgtatc cgctacttcc tgcaccagtt ggactacccg ggtaaagacg tgaaagccat
     4681 cggcaaagtg gacgataaaa tcgttcttgt tcccgatacg cgttacaaag agaaaaccat
     4741 cgatgtcggt catgactgat tgccggtgaa gtaataaaaa tgccgtctga acccgatttc
     4801 aggttcagac ggcattttct atccgtgtgt caaaccggca tcagatgtcg gtttccagat
     4861 aaatgacttg ggtttgcagg tattcttcca aaccgtgttt gccgtccgcg ccgccgatgc
     4921 cggatttttt ccagcccgcg tggaagccct gcatggcttc gaagttttcg cggttgatgt
     4981 aggtttcgcc gaattgcagg cggcgggtaa cgtagaaggc ttcgttcagg ttggtggtgt
     5041 acacggagct ggtcagaccg aattcgcagt cgtttgccag ggcgatgacc tggtcgagcg
     5101 tgtcgaaagc ggaaacgggc agcacgaggc cgaaagtttc ttctttcata atgtccatac
     5161 tgttgtcggt gtcggtcagc agggtaggct cgaagaaata gccgcgtcct tcggtgcgtt
     5221 tgccgccgca aaccaatttc gcaccttgtt tgacggcccg ttccactttt tcggcaacgg
     5281 ctttgacggc gcgctcttca atcagcgtgc ccatttccgg cgcgcctgct tcggcttcgg
     5341 cagggttgcc gtagcgcacg cctttcatcg cggcggtcat tttttcaatg aacgcgtctt
     5401 tcaggctgct gtggacatag acgcgctcgg cgcagttgca gatttgaccg gtgttgccga
     5461 cgcgcgaagc caagatggat ttcaccgcta aatccaaatc cgcgtctttc aacacgatgg
     5521 cgggggcttt gccgccgagt tccagcgaga ctttggtgat gttggcggaa gcggcttcca
     5581 tcacttggcg acccgcttca accgagccgg tcaggctgac catatcgact tgcggatggg
     5641 cggacaaggc gttgccgatt tccgcacccg gaccgtccac cacgttgaac acgcctgcgg
     5701 gcagtccgac cgcatcgatg atttcggcga agatgtggca gttgatcggg gttacgctgc
     5761 tgggtttgac gacgatggtg ttgcccgtaa ccagatccgg gcccattttg cgggcgatca
     5821 ggaagaaggg gaagttccgc ggcaaaatgc cggcaatcac gcccagcgga cgtttgaacg
     5881 ataaaatgtt ttcgcgcggg cggtcgcttt ggatgatttc gccttcgtaa cggcgcgccc
     5941 attcggcttg gtaatcgaga tagtcggcag tgaacatcac ttccacacgc gccaagtctt
     6001 tggttttgcc gccttcggca acgatggtgt cggtcagctc gtcggcgcgt ccgcgtatgc
     6061 cttgggcgat tttacgcaaa tacgcgccgc gttcgaccgc aggcagacgc tcccaagccg
     6121 gttgcgccgc gcgcgccgcg gtcaacgtcc gccctgccgc ctttgggttc gcgggcgatg
     6181 gtttcttcgg tggacgggtt caatacgtcg cgccattcgc cgttgaaatc gttttcaaag
     6241 cgtccgttga tgtacatggc caattgtttc atttcgggcc ctccagtttt gtagtcggat
     6301 gtagttttgg tttattccca aataaattgc cgtacaagtt tctttacacg cggattttgg
     6361 gtttcaagtc aaatacggct taccggtttt cttttttagt ttccgcaggc gtgctgccga
     6421 aatattgcct gaatgcctga ataaagctgg aaacgtggcg ctagccgcac aaatacgcgg
     6481 tttcgcccac gtttttgccc ccgttttgca acagatagag cgcgtgctgc atttgtttgt
     6541 ggtgcagcca ctcgcttgcc gtaatgccga aatggtcgcg catacggcgt tgcagcgtcc
     6601 tttcgctgat gttcagcgcg gctgtcagcc ggttgacttg gtgtgcgccg ccgtcgaacg
     6661 cggcattcag ggtgcggctg aagtcttcag acggcatagc gtctgcttcc gccgtttgcc
     6721 ccgccgccgg ctcgatgccg tctgaaaccg tgtcccacaa atccgacagc agccgcaaca
     6781 cgtccgcctc gcggcgcaat gtttcgccca aatgcccctt tgggacggtt tgcaggcagg
     6841 atgccgccaa gccgcgcagg tttgggggca aatcccatat cctgaccggt tcgcggtaaa
     6901 gcaggggtgc gaaacgcgcg tattccggac gcggcagcca ttgttccata cctttaatgg
     6961 tcattttgac cgttttgccg ccgcgataaa gatagcggct gaacaggact tcttccccga
     7021 cggcaattag gacaatcttg ccgccgtccg catcgatgcg gaagcggcgg cggttgatgc
     7081 cgaagtccaa acgcccttcc agcaacagca cgaacgacac ataaggttcg gcgaggcggc
     7141 tgctgcaaaa atcgcaccgt gccgttaccg taccgccgtg gatggaaatg ccgttggaca
     7201 aagtatcgaa acggtagctg ccctccacat aagccgccgc cgcttttgac cagattgacg
     7261 aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag gttatcattt
     7321 gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaaaggca ttttcacaac
     7381 acttgccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct taaaatacac
     7441 gccgtcaaac ctaccgtccg cccgtacatg agcccatccc cctttatcga aatgaaagac
     7501 gtcgccttcg cgtatggcga ccgcccgatt ctgaacgaca tcaatttcag cattccgcaa
     7561 ggcaattttg ccgccgtgat gggcggttcg ggcagcggca aaaccacgct gatgaggctg
     7621 attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg ggatttggcg
     7681 ggtttttcgg ctgacgaact ctacgaacac cgccgccgta tgggcgtatt gttccaacac
     7741 ggcgcgctgt ttaccgattt gtcggtattc gacaatatcg cttttccgat gcgcgaactg
     7801 acgcaacttc cggaagcggt tattcgcgat ttggttttgt tgaaattgaa cgcggtcggt
     7861 ctgcgcggcg tggaaaacct gatgccgtcc gagttgtccg gcgggatgtc gcgccgcgtc
     7921 gcgctggcac gcacaatcgc gctcgacccc gaaattatgt tgtacgacga accgtttacc
     7981 ggcctcgacc cgatttcctt gggcgtgatt gcccacttga tcagccgcgt caacaaggcc
     8041 ttgcgttcga ccagcattat ggtaacgcac gacattgaaa aatctttgga aatcgtcgat
     8101 caggtaattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca ggaaatgcgc
     8161 gaactggatt cgccttgggt gcgccagttt gtcggcgggc tggcagacgg ccccgtggca
     8221 taccgctatc cggcgcaaac gtcgttgcag caggatttgc tcgggtaaat gagcaggtgc
     8281 cgtctgaaac cgttgaacct tacatataga aatttatgaa ttttatccgt tccgtcgggg
     8341 cgaaaaccct cggccttatt caatccttcg gcagtatcac gctgtttctg ctgaacattt
     8401 tggcgaaatc cggcacggct ttcgcccgtc cgcgcctgag cgtgcgccaa gtgtattttg
     8461 ccggcgtgct gtcggtgctg attgttgccg tttcggggct gttcgtcggt atggttttgg
     8521 gtttgcaggg ctatacgcag ttgtcgaaat tcaaatccgc cgatattttg ggctatatgg
     8581 tcgcggcttc tctgttgcgc gaactgggtc ccgtgttggc ggcgattctg tttgccagca
     8641 gcgcgggcgg tgcgatgacc agcgaaatcg gtttgatgaa aacgaccgga cagctcgaag
     8701 cgatgaacgt gatggcggtc aaccccgtcg cccgcgtggt tgccccgcgt ttttgggcgg
     8761 gcgtgttttc tatgccgctt ttggcttcga ttttcaacgt cgcgggcatt ttcggcgcgt
     8821 atttggtcgg cgtgagctgg ctgggtttgg acagcggtat tttctggccg cagatgcaga
     8881 acaacattac gatacattac gatgtaatca acggtttgat caaatccgcc gcgttcggcg
     8941 tggcggtaac gctgattgcc gtgcatcagg gcttccactg catcccgact tcggaaggca
     9001 ttttgcgcgc cagcacgcgc acggtggttt cgtccgccct gacgattttg gcggtcgatt
     9061 ttatattgac cgcgtggatg tttacagatt gacagactgt ctgaagacga aacgcggaac
     9121 atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg tcggactgtt
     9181 cgtcctgatc ggcgcggcgg cggttgcctt tctcgctttc cgcgtggcgg gcggcgcggc
     9241 gttcggcggt tcggacaaaa cttacgccgt ttatgccgat ttcggcgaca tcggcggttt
     9301 gaaggtcaat gcccccgtca aatccgcagg cgtattggtc gggcgcgtcg gcgctatcgg
     9361 gcttgacccg aaatcctatc aggcgagggt gcgccttgat ttggacggca agtatcagtt
     9421 cagcagcgac gtttccgcgc aaatcctgac ttcgggactt ttgggcgaac agtacatcgg
     9481 gctgcagcag ggcggcgata cggaaaacct tgctgccggc gacaccatct ccgtaaccag
     9541 ttctgcaatg gttctggaaa acctgatcgg taaattcatg accagcttcg ccgagaaaaa
     9601 cgctgagggc ggcaatgcgg aaaaagccgc agaataaaca tacacatcca ttttcaggaa
     9661 agacatcatg aaaaaatcct ccttcatcag cgcattgggc atcggtattt tgagcatcgg
     9721 catggcattt gcctccccgg ccgacgcagt gggacaaatc cgccaaaacg ccacacaggt
     9781 tttgaccatc ctcaaaagcg gcgacgcggc ttctgcacgc ccaaaagccg aagcctatgc
     9841 ggttccctat ttcgatttcc aacgtatgac cgcattggcg gtcggcaacc cttggcgtac
     9901 cgcgtccgac gcgcaaaaac aagcgttggc caaagaattt caaaccctgc tgatccgcac
     9961 ctattccggc acgatgctga aattcaaaaa cgcgaccgtc aacgtcaaag acaatcccat
    10021 cgtcaataag ggcggcaagg aaatcgtcgt ccgtgccgaa gtcggcatcc ccggtcagaa
    10081 gcccgtcaat atggacttca ccacctacca aagcggcggc aaataccgta cctacaacgt
    10141 cgccatcgaa ggcacgagcc tggttaccgt gtaccgcaac caattcggcg aaatcatcaa
    10201 agccaaaggc atcgacgggc tgattgccga gttgaaagcc aaaaacggcg gcaaataacc
    10261 gacaaaccat accgtccgag ggcggtatgg gtttttcgaa caaacgctat gcatacagaa
    10321 ctcaaaaacg gaacactgca catcggcggc gacatcaccg tcaaaaccct gaccggggac
    10381 gcatttgggc gtttccggca acaatgccgt ctgaaagaaa ccattgccgt cgatttcgga
    10441 ggtgtcaaac gtgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct gcgcggctgc
    10501 aaaggcagcg tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc cgaactgtac
    10561 gaaatcaaag actggctgaa atcatgaaaa aaaccgccta cgccatcctc ctgctgatcg
    10621 ggttcgcttc cgcccctgca tttgcagaaa cccgccccgc cgacccttat gaaggctaca
    10681 accgcgccgt ttccaaattc aacgaccaag ccgaccgcta cattttcgcc cctgccgcgc
    10741 gcggctaccg caaagttacg ccgaaacccg tccgcgccgg cgtgtccaat ttttttaaca
    10801 acctgcgcga cgtggtcagt ttcggcagca atatcttgcg tttggacatc aaacgcgcaa
    10861 gcgaagacct cgtccgcgtc ggcatcaata ccaccttcgg tttgggcggg ctcattgata
    10921 ttgccggcgc gggcggcgtt cccgacaata aaaacacttt gggcgacacg tttgcctcgt
    10981 ggggctggaa aaacagcaat tatttcgtgt tgcccgtctt aggcccgtcc accgtccgcg
    11041 acgcgctcgg cacgggcatt acctctgttt atccgcccaa gaatatcgtt ttccataccc
    11101 ctgccggacg ctggggcacg actgccgctg ccgccgtcag tacgcgcgaa ggcctgctcg
    11161 atttgaccga cagtctggac gaagccgcca tcgacaaata cagctacacg cgcgacctct
    11221 atatgaaagt ccgcgcacgg cagaccggtg caacacctgc cgaaggtacg gaagataaca
    11281 tcgacatcga cgaattggtc gaaagtgccg aaaccggcgc ggcagagccc gccgttcacg
    11341 aagattccgt atccgaaaca caggcagaag cagcagggga agccgaaacg caacctggaa
    11401 cacaacccta aagccatgcc gtctgaaaat ccgtgttcgg acggcattcc cttccgtcgg
    11461 aacggacagt ccgacactac agattgggct ataatccctt ttttgcgcat accgcgcgac
    11521 agcaaaggaa aaaccatgta cgaagtcaac cgcagcgtat tcgtcctcat ccctttagaa
    11581 cctttttgga actggctgca aaccctgccg ggcaaccacc tcgacggact gaccctcgaa
    11641 gacatccaag ccgatgccaa ttcctacctt gtccgcccgt gcgaaaccgc cgacgaagta
    11701 tgggacgaaa tcgaagcccg tttcgaagac attttcgccg ccgaacttgc cgactggtgc
    11761 gaagacgaac gcgagtggcc cgcactcgat gccgacattt tcaacgaatg gttcgacatc
    11821 cagctttcta ccgtcatcac cgacctcgaa cacgaaccgc ttgcccgcga agccttccaa
    11881 cccatcaacc tgaactgatg aagctgaccg tccgcaatta ccatctcgac ggctacggtc
    11941 atgtcaacaa tgcacgctac ctcgaatttc tcgaggaagc gcgctgggcg ttttttgaaa
    12001 aacgcggact gatgcacgag ttggcaggcc tcatactgat tgtcgcccgc atcgacatcc
    12061 gatacagccg tcccgccgtc gaaggcgacg tattgcagtt ttcatgccgc ctgaaaaccc
    12121 ccggcatgcg ccgcattgtg ctgacccaga cgattacact gccaaacggc aaaaccgccg
    12181 cagaagccga catcaccctg atgcccgtcc acgccgccac acaacgcacc gtcagcctgc
    12241 ccgccaccct tgcccgcgca ctggaagcct tgtccgaatg aaaaaaatac tcaccgccgc
    12301 cgccgtcgca ctgatcggca tcctccttgc caccgtcctc atccccgaca gtaaaaccgc
    12361 gcccgccttc tccctgcccg acctgcacgg aaaaaccgtt tccaacgccg acctgcaagg
    12421 caaagtcacc ctgattaatt tttggtttcc ctcctgtccg ggttgtgtga gcgaaatgcc
    12481 caaagtcatc aaaacggcaa acgactacaa aaataaagat ttccaagtcc tcgccgttgc
    12541 ccagcccatc gatccgatag aaagcgtccg ccaatacgtc aaagactacg gactgccgtt
    12601 taccgtcatt tatgatgcgg acaaagccgt cggacaggca ttcggcacac aggtttatcc
    12661 gacttccgtc cttatcggca aaaaaggcga aattctcaaa acctacgtcg gagaacccga
    12721 tttcggcaaa ctctaccaag aaatcgatac cgcgctggcg caatagcgat gccgtctgaa
    12781 gcgcaaaaaa cagaaacgat gttcctgttt tttcagatca tccgccccga cacgttcagg
    12841 cggcattcaa ccttccggca gattcttttt caccaccgca ggattgcctg ccgccaaaga
    12901 gtagggcgga atgtcttttg taatcaccgc gcctgcgccc acgaccgagc cgcgtccgac
    12961 ggttacgccc gccatcacaa tcaccctgcg ccccggccag acatcgtcct ccaacgtaat
    13021 cggacggatt tccgtgtagc cctcaaagcg tttttttcac ggtcaaactt gtggttggtc
    13081 gaatagagca ggcattccgg ccccatcatc acatttttgc cgaccaccag cccacggcag
    13141 atttcacagt ttgccccgat gcccgagccg tcgcccaaaa ccgtatccgg aaacacatac
    13201 gccccgcgtc cgatatgcgg cgaaatccgc cgcgccagaa aaccgagcac ccgtctgccg
    13261 atgatgccga tgccgcgcat atgcgacggc ggcaacaccg aaccggcaaa acgcagcagc
    13321 aggaaaaaga attttgatcc catcaaacct ccctcaaaag aactttttat agtggattaa
    13381 caaaaatcag gacaaggcga cgaagccgca gacagtacag atagtacggc aaggcgaggc
    13441 aacgctgtac tggtttaaat ttaatccact atacatttta tcgaatatcc tcttgacgaa
    13501 acaacagaaa atagcgagaa tgccgagctt gtctgaggca taggtacatc ccctatgccc
    13561 gcttttttga aaggaaatca aatgaaacaa ggtattcacc cgaactaccg cgaagttaac
    13621 gttacctgct cttgcggcaa caaattcgta accaaatccg caatggaaaa agaaaacttc
    13681 aacatcgagg tttgctccct gtgccacccg ttctataccg gcacccaaaa aatcgtcgat
    13741 accaccggcc gcgtggacaa attcaacaac aaattcggca acctgttcaa acgctgattg
    13801 ccgctttgca agaattaagc cctgccatca tttcgatggc agggcttttt aatatttggc
    13861 acactgactt cttatctata tagtggatta acaaaaatca ggacaaggcg acgaagccgc
    13921 agacagtaca aatagtacgg caaggcgagg caacgccgta ccggttttgt taatccacta
    13981 tattttgacg gggttgaagt tgacatctac aaagtttaat gtttgaatac attaaaaaat
    14041 atatttgaat gggatgtttt atgattcaaa atgttgttac ttcaataatc ctgtattctg
    14101 ggacagccgt agacttactt attatcctaa tgttattttt tgccaaaaaa aaagcggaaa
    14161 agacatcatt aacatctatt taggacaatt tctaggctct gttagcctga tattgctaag
    14221 tttgcttttt gcatttgtct tagattatat tcctagtaaa gagattttag gtttgctcgg
    14281 cttgattccg attttcctag gcctcaaagt tttgctgtta ggagattccg atggagagtc
    14341 tattgccaaa gagggtttgc gcaaagataa taaaaacctg atttttctag tcgctatgat
    14401 tacttttgca agttgtggcg ctgacaatat tggtgtcttt gtcccatatt ttactacctt
    14461 aaatttagcg aatttgatag tggctttact tacctttcta gttatgattt atctcttggt
    14521 tttttctgcc caaaaattgg cacaagtccc ttctgtcgga gaaactttgg aaaaatatag
    14581 cagatggttt gttgccgttg tttatttagg attggggata tatatcctga ttgaaaacaa
    14641 cagttttgat atgctatgga ctgtgtcggg ccaggaaaaa atattatgaa aaagatagta
    14701 tctgcaaaga ctgccccgtg cagtcttttt tgttgccggt ctttttgtgt ctgatgccgt
    14761 ctgaagcagt ctctgcacga cctttgtgcg aatatttgct acacttggca atccgtccat
    14821 ttcttct
//
LOCUS       DAWTHD010000059        14739 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWTHD010000059 DAWTHD010000000
VERSION     DAWTHD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.059"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            183..2189
                     /locus_tag="WM704_001336"
     CDS             183..2189
                     /locus_tag="WM704_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFA9340750.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2312..3403)
                     /gene="mltB"
                     /locus_tag="WM704_001337"
     CDS             complement(2312..3403)
                     /gene="mltB"
                     /locus_tag="WM704_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFA9340751.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3551..4978)
                     /locus_tag="WM704_001338"
     CDS             complement(3551..4978)
                     /locus_tag="WM704_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFA9340752.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5163..8867
                     /gene="mfd"
                     /locus_tag="WM704_001339"
     CDS             5163..8867
                     /gene="mfd"
                     /locus_tag="WM704_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFA9340753.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDGIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDDPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQKINAIHEELVDRFGLTEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9093..9395
                     /locus_tag="WM704_001340"
     CDS             9093..9395
                     /locus_tag="WM704_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFA9340754.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9557..9940
                     /locus_tag="WM704_001341"
     CDS             9557..9940
                     /locus_tag="WM704_001341"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFA9340755.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9962..10804
                     /gene="kdsA"
                     /locus_tag="WM704_001342"
     CDS             9962..10804
                     /gene="kdsA"
                     /locus_tag="WM704_001342"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFA9340756.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10819..11259
                     /locus_tag="WM704_001343"
     CDS             10819..11259
                     /locus_tag="WM704_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFA9340757.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11306..12592
                     /gene="eno"
                     /locus_tag="WM704_001344"
     CDS             11306..12592
                     /gene="eno"
                     /locus_tag="WM704_001344"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFA9340758.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12760..13038
                     /gene="ftsB"
                     /locus_tag="WM704_001345"
     CDS             12760..13038
                     /gene="ftsB"
                     /locus_tag="WM704_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFA9340759.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13079..13369)
                     /locus_tag="WM704_001346"
     CDS             complement(13079..13369)
                     /locus_tag="WM704_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HFA9340760.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13490..14623)
                     /gene="nrdB"
                     /locus_tag="WM704_001347"
     CDS             complement(13490..14623)
                     /gene="nrdB"
                     /locus_tag="WM704_001347"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HFA9340761.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
BASE COUNT         3557 a         4299 c         3642 g         3241 t
ORIGIN      
        1 ccgaaattgt tataatcgaa aaaattaaaa atcaatacgg attattctat cagattgatt
       61 tatcgatatt tattatatca ttaatatagt ttgatttcaa accggcggaa aatgcgccgc
      121 ccgccgcaga tgcgccctcc ccgccatcgg ggcgtttaac gccggattgc cgatgcggta
      181 caatggcaga tatgaagaaa attacccctc aaaacctgcg tcccctgctt tcggaaagct
      241 tgggacatac cgattttgtc aacgtcctca acgcattgat taaatttttg cgccgtggcg
      301 gcaaaaaatg tgcgggggag cgtttcgacc tgattatcga cacattcaaa caagacaggg
      361 aattactgtc ccgcttcagc cggtgttttt acatctggct cgcgcaaata cacatttatc
      421 cggcactcat caaactcggc attttctcgc gccacagctt tgcccgggaa atgggcatac
      481 gcatatacga acgcttcaac ccgtcatata aagattttgc caacttgggc gaagtcttcc
      541 tttatctttt ccattccgaa aacgacgaca aatggctgca aacgctcaat atccgccaat
      601 ggctggtttt atacgaactc atccgtagcc acgccgagcc gtccaaattg cagacggcgg
      661 gcatccgcct tgccgatgcg cgtttgcgcg ccatcgaaat gctgtctgtc tggacggcat
      721 ccgaagccat cgaacccgat ctcatccgta tcgccccgcg cctgctggaa gccgattctt
      781 ccttcgtcgc cctccaacgc gaaaccgcca aactggtcga acactaccgc aacggcaccg
      841 cgccttacga caccgcccac ctcgaagtga tgttcgacca atgtttcagc cagattgact
      901 atttgcgtcg caaagggacg ggcgccggct ccggttcgtc ggtcaaagtc gcccacctgc
      961 tcgaacgact ccggcagacc gtaggccgtc tgaagctgct cacagacata caaaccggtg
     1021 ccggcaacag caaccgcctg accatcgccc tgatgaactc cctcatctac gcggcggtcg
     1081 aacaatacag cacccgccac ctgcgccgca gcagcatccg tatgctcgcc cgcagcatta
     1141 ccgaaaacaa aagccaccac ggcgaacact acatcacccg caaccgcaaa gaatatttca
     1201 aaatgttcta ctcggcagca ggcggcggcg gcatcatcgc cctgatggcg ctgctcaaaa
     1261 tccgcatcgg cacactcgac ctcagtccct tcctcgcttc cttgtcggct gggttcaact
     1321 acggcatcgg ctttatgatc atccatatgc tgcactgcac cgtcgccacc aagcagcccg
     1381 cgatgactgc cgccagcttt gccgaacagg tcgatctcaa cgaaggcggc aaagcggtgg
     1441 acaacaaact ctccaagctc ctcatcgacg tatgccgctc ccaaagcgtc gccgtcttcg
     1501 gcaacgtttc catcgccatc cttttggcgt gcgccatatc gttcggctat gcccatctgt
     1561 accggctgcc catactcgat gcccacaccg ccgcctacca gttcaaatcc atagacatca
     1621 tcaatcatcc taccctatgg tatgccgcta ttgcaggctt gtggctgttc tgctccggca
     1681 tcatcgcagg ttttttcgac aaccgcgccg actacctcaa cctgcgccaa cgcctgccct
     1741 tcaatccctt gctgcgtaaa atcatgcgcc ccgggccccg ccgcgtcctc gccgcctaca
     1801 tccacaaaca ctacggctcg ctggtcggca acttcatctt cggaatgctc ttgggtatga
     1861 ccggctattt cggacacctc ctcgggctgc cgctggacat ccgccacgtc gccttttcct
     1921 ccgccaacct cggctatgcc gccgtcagcg gcaacgtcgg ctttggtaca ttcgtactcg
     1981 gcattttcag cgtcctcgcc atcggcttgg tcaacctctg cgtcagcttc agcctcgccc
     2041 tcttcgtcgc cctgcgctcg cgcggcacaa aaatcggcag catccgcaac ctgatcaaaa
     2101 gtttttggaa tcagattaaa agcaatcctt gcatactttt cctcccgccc gccaaaggac
     2161 agggacatcc tccttcggac aagccttgac cggcaatgcc gtctgaaacg ggattcgccc
     2221 cgaataccgc cctgatgcgg gaaatcccca taaaaggatg caaaaatgcc gtctgaaccg
     2281 aaacgtggtt cagacagcat tttaaaaaac attacaatcc cgggccgccg agcgaattgg
     2341 caatgtccct gaccgccgtt acatacatcc ggctgtggtt gtactgccat accgtataaa
     2401 aattgttcaa gcccaaataa tattcaaaca cgccgggtgc ggtttccagt ttgaacaaaa
     2461 ccgccttttc atcatctgcg agcgtttccc cggggatgat gccgtacgcc ttcaaatccg
     2521 ccaccgtccg cgtcagggcg gttttttcgc caatgattgc ctgaacatcc gcacccggcg
     2581 ccaacgtcgc cgacaccaac attttaccgc ccgtgcgcca accgtgctgc ttcatataat
     2641 tggcaaccga tgccgcgaca tcaccgacgt tgccccatat atcccgatgt ccgtccccgt
     2701 cataatccac cgcccatttc cggtagctcg aaggcataaa ttgcggcata cccattgcgc
     2761 ccgcatagct gcccttaaag gcgaaaacat cgccgccttc ttcttttgcc agctttaaaa
     2821 gctcgaccaa ttctttttgg aaaaacccgg cgcggcgggg ataatcaaag cctaaggtcg
     2881 ccaatgcgtc cgccacacgg aagctgcccg tatttttgcc gtaattcgtt tcaatcccga
     2941 taatcgccac gataagctcg gcaggcacgc cgtatttttg cgccacatca tcgataaccg
     3001 cgcggttttc cgcataaaac ctgcgcgcgc cgtgaaattt cgccctgccc gaatttcccg
     3061 tgcggaacac ataccacgga cgcgatgtgg agggtcggtg cataatcttg acgatgtccg
     3121 ccttgtaagc cgctttgtca aaaaaatcct gccattccgc ctgggaaaaa tcccctttcc
     3181 cgacttcatc gtccacaaaa cggcggacat ttgcattggc ggcaaacccg ctgtcggata
     3241 ccggtacggc tgccgcgtca aacgcggggc ggctttcttt tttcatttca tccgcgcggg
     3301 gggcttgggc ttcatttgcc cggggtgtgc gggcctccat cgccgtacag gcagacaaag
     3361 ccgccaaaca aattgccagc ggcagtattt ttctcttttc cataaacaca ttccgaacaa
     3421 atagggtaag tgggaaagcg gcacaagggt gaggcgcgaa atgcggtatc cgccgcaatc
     3481 ggcggttttg cagaatgccg cgcgttgcct tttgcgccgc ccgaaatctg cctgccgccg
     3541 tatttgcccg ctatccgcaa tatcggcagt ccaatatatc tttgcggatg tcgttcagca
     3601 ggaaggctgt ggtgtcttcg tgttcctcct gtacgaagac aaagagtcgg gcgatgattt
     3661 tgccgatgaa gactttttgc agggcgcagg cgtcggtcag ggtgtgttcc tgcaggaagc
     3721 tgcggatgtc ttcgggcaaa gcgtagtcgc gggcaacggc ggcaaagcgg acatcggttt
     3781 gcaggcggtc gagcagggtt tggtttttgt ccaacttaat gcctgcttct ttttcttcgg
     3841 cggtggcgcg gacgaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa
     3901 aaatcgtgaa ggggcggata tcgatggcga tattgccggc tgggtgtccg cgttcaaaac
     3961 ccttcgcgcc caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga
     4021 gggttttgac gatgttcgcc tccatcaatt gatgggcgac gggcgcgacg ggcgaaacgg
     4081 aatggcagac gtagcggtaa aggatttcgg aaacctgatg gcggcgttgg atttcgcggc
     4141 gttcgtaatc gacgaatctg atatcgttgc ggacatatcg gttcaggttg tcaaggatgt
     4201 attccataat gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt
     4261 ggaacgcgcg caaaccggca gcgtcgcccc gggagagttt catcacggcg gttgcaggca
     4321 tttcggcatc gatgcggttg acggcgtaac ggacggcgcg caggccttcg gatgcgaggg
     4381 tttcgcagcg gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc
     4441 cgtttttgcg ctctttggcg gcaacgagga ggaagtcgct ttgcgaattg ccctgccagt
     4501 atttcgcggc gttgacgtaa atggtttgtt cgtcggtata ttcgtagcag gactgcattt
     4561 cgcgtgcaat cgccgcgccg gaggtttcgg gttcggtaac gcccaaaccg ccgccttcgc
     4621 ctttgaaaat catgtccaaa ccttgtgcga cttgcgcttc gccgccgaac tcttgcagag
     4681 gctgcaacac cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc
     4741 ccgcaatcct taggacttct tggatttcaa actgactgcc cttgcgcccg ccgtgttttt
     4801 tgtcgaggaa gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca
     4861 ggtatcgcat caggtcgatg ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct
     4921 ttaaaaaagc agccgtgtcc atggttgacg gctgcgcgct cggttcggtg tgtatcatcg
     4981 gcttcctctg tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag
     5041 ttaattttct aaactttacc acaaagtgca ccgggcaaca atccgccggc ctttcagacg
     5101 gcatcgcgca ccctcacgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca
     5161 ctatgacctg ccccatcccc aaaccccgtg aaaaatcccg ttggttcaat ctttcgcaag
     5221 gctcgctgcc cttggctttg gcgcgttatt tgccgcacaa gcggctcaaa gccgtgctga
     5281 cccaagatgc ggaacaggcg ttgcgccttc agacggcatg gcggtttttc cgtccgcacg
     5341 acacggcggt gttcctgccg gactgggaaa cgctgcctta cgagcgtttt tctccgcatc
     5401 aggatttggt gtcggagcgg ctgtcggcgt tgtggcagat taaaagcggc gcggcggacg
     5461 tgttgttcgt accggttgcc acggcgatgc agaagctgcc gcccgtgccg tttttggcgg
     5521 ggcgcacgtt ttggctgaaa acggggcaga ctttggatat aggccgcctg aaaaccgatt
     5581 tggtggatgc gggctacaat catgtttccc acgttgtcgc ggcgggcgag tttgccgtgc
     5641 gcggcggcat tgtcgatttg ttcccgatgg gcagcgaaac gccgtaccgc atcgatttgt
     5701 ttgacgatga aatcgacggc attaaaacct tcgataccga cacgcagcgc accatttccc
     5761 ccgtttccga aatccgcctg ctgccggcgc acgagttccc caccgacagc gaggcgcaaa
     5821 aaatcttccg cagccgcttc cgcgaggaag tcgacggtaa tccgaacgat gccgccgtgt
     5881 acaaagccgt cagcaacggc cacttcggcg cgggcgtgga atattatctg ccgctgtttt
     5941 ttgaaaacga gctggaaacg ctgtttgact atatcggcga agatgcgctg tttgtctctt
     6001 tgggcgatgt tcatgccgag gcaaaccgct tttggaacga cgttaaatca cgttacgcca
     6061 tggcgcaggg cgacgaaacc tatccgcctt tgcttccaca gcatttgtat ctctctgccg
     6121 atgtgttcgc aggccgtctg aaaaactacg gacaagttct gcctgatgtt tccggcaagg
     6181 cacactccct gcccgacctt gccgtcaacc gccaatccga cgatcctttg caggcattga
     6241 aggattttca gacggccttt gacgggcgga ttttgctgtg cgccgaaagt ttgggacggc
     6301 gcgaaaccat gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact
     6361 ggcagggctt cttgtcggcg cacgagccgc tgatgattac ggtcgcgccg ttggcttacg
     6421 gattcaaact gggcggactg caatcttcaa gccaacagca aactgttccc gcctccgagg
     6481 gagaaggcaa agcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc
     6541 ccagccctct cccgcaggag agggaacaaa gtgccgccgc cgtttcagac ggcctgaaag
     6601 cagccgccgt ttcaaccgaa agcagcctgt atctcgtcgc aagtgatctg cacgggcaaa
     6661 cccgacagca atctgccccc tcccccgtgg gggagggttg gggagagggc aaagcggttg
     6721 ccgctcaaag tgccatcgcc gtcatcaccg aatccgatct ttaccaatac gtcgcccgtt
     6781 cgcgcgtcca caaccgccgt aagaaacacg ccgctgtttc agacggcctg ttgcgcgacc
     6841 ttgccgaaat caatatcggc gatcccgtcg tgcacgaaga acacggcatc gggcggtata
     6901 cgggcttggt aacgatggat ttaggcggcg aaaccaacga aatgatgttg ctcgaatacg
     6961 cgggcgaagc gcagctttat gtgcctgttt cgcaactgca tttaatcagc cgctactccg
     7021 gtcaggcgca tgaaagcgtc gccctgcaca agctcggcag cggcgcgtgg aacaaggcga
     7081 agcgcaaagc cgccgaaaaa gcgcgcgata ccgccgccga gttgctcaac ctctacgccc
     7141 aacgcgccgc ccaatcggga cacaagtttg aaatcaacga gtcggactat caggcgtttg
     7201 ccgacggctt cggctacgag gaaaccgaag accaggccgc cgccatcgcc gccgtgatta
     7261 aagatttgac gcaggcgaag ccgatggacc gacttgtgtg cggcgatgtc ggtttcggca
     7321 aaaccgaagt cgccctgcgc gccgcgtttg tggcggtgat gggcggcaaa caggtcgccg
     7381 tactcgcccc gaccacgctt ctggtcgagc agcacgcgca aaacttcgcc gaccgtttcg
     7441 ccgatttccc cgtaaaagtc gccagccttt cgcgtttcaa caacagcaaa gccaccaaag
     7501 ccacgctcga aggcatggcg gacggtactg tcgatatcgt tatcggtacg cacaaattgg
     7561 tgcaggacga cataaaattc aaaaacttag gtttattgat tatcgatgaa gaacaccgct
     7621 tcggcgtgcg acagaaagag cagctcaaac gcctgcgcgc caatgtcgac atcctaacca
     7681 tgaccgccac gccgattccg cgcaccctca gcatggcgct cgaaggtctg cgcgacttct
     7741 cgctgattac cactgcaccg agccgccgcc tcgccgtgaa aacctttgtc aaaccgttca
     7801 gcgaaggcag cgtgcgcgaa gccgtgttgc gcgaactcaa acgcggagga caggtatttt
     7861 tcctgcacaa tgaagtagat acgattgaga atatgcgcga gcggctggaa accctgctgc
     7921 ccgaagcccg tatcggcgtg gcgcacggac aactgcgcga gcgcgagctg gaacaagtca
     7981 tgcgcgactt tttgcagcaa agattcaatg tgttgctctg ttccaccatc atcgaaaccg
     8041 gcatcgacat ccccaacgcc aacaccatca tcatcaaccg cgccgacaaa ttcgggctgg
     8101 cccaactgca ccagcttcgc ggacgcgtcg gccgcagcca ccaccaagcc tacgcctacc
     8161 tgctcacgcc cgaatacatc accaaagacg cagaaaaacg cctcgacgcc atcgcggcgg
     8221 cagacgaact cggcgcaggt ttcaccctcg ccatgcagga tttggaaatc cgaggcgcag
     8281 gcgaaatcct cggcgaagga caatccggcg aaatgatgca ggtcggcttc acgctctaca
     8341 ccgaaatgct caaacaggcc gtgcgcgacc tcaaaaaagg ccgccaaccc gacctcgacg
     8401 caccgttggg cattaccacc gaaatcaaac tgcacagccc ggcattactg cctgaagact
     8461 actgccccga catccacgag cgcctcgtcc tctacaaacg cctcgccgta tgcgaaaccg
     8521 tgcagaaaat caacgccata cacgaagaac tcgtcgaccg cttcggcctg accgaacaac
     8581 ccgtcaaaac cctcattgaa agccaccacc tgcggcttgc ggcaaaagaa ctgggtattg
     8641 atgccatcga tgccacaagt gaagccgtaa ccgttacctt cggcaaacac cactgtatcg
     8701 acccgacggg gattatcctg ctgattcaga cggataaaaa ataccggctg gcaggggcag
     8761 acaaattgag gtttgcagcg gaaatggaaa acatcgaggt caggatcaat accgtcaaaa
     8821 ccgtactaaa aaccctgcaa ggcaaacgcc tgcccaaagg aaattgatgt aacgatgccg
     8881 tctgaagaac accgttcaga cggcatacaa tcccccaatc ctcaccaaaa ggaacaatca
     8941 tgtcgatttt tcacccctat ttccaacaac tgtccaccga aggttttgac ggagaagaag
     9001 atgtcctctg ggaagacgag ctgaccgtca aaggcaatct tgtcgaggta ttcctgtagg
     9061 cagaaaatga aaacagcctg ccaaccgaaa cattggatgc atttgccgcc ttcctgtccg
     9121 aattggaaaa agccgacgat gctgcacgcg ccgcactgac ggaatatttg aaacgggaca
     9181 gccgctatat cgactttcac accgaagata cccaaacaag ccgtgatgcg gcaaaatttg
     9241 tccgcactat gcagttgatc tatatcagct tatgggcaaa aaatccggct tttgccgtta
     9301 tggattatat gcccgccaat atggaaagcg acgaaatcct tgccgtcaaa ctgcatttgg
     9361 acggcagcat tttctcaatc gattgggaga gttgagccgg attgccaatc ggcaaaaatg
     9421 aaatgccgtc tgaaaacagc ttccctgccc gaagccttgc cattatgaat ttgaaggaaa
     9481 ctccactata atacggcatt cagattttca gacggcatca cgcccgtcag accgcacaca
     9541 aaccaaaagg aaatacatgt tccgtactat acttggcgga aaaatccacc gcgccaccgt
     9601 aaccgaagcc gatttaaact acgtcggcag cattaccgtc gatcaagacc tgttagacgc
     9661 ggcaggcatc tgccccaacg aaaaagtcgc catcgtcaac aacaacaacg gcgaacgttt
     9721 tgaaacctat accattgcag ggaaacgcgg cagcggcgtg atttgcctga acggtgctgc
     9781 agccaggctg gtacagaaag gcgacatcgt catcattatg tcttatatcc aactttccga
     9841 accggaaatc gccgcacacg aacccaaagt cgtcttagtg gacggaaaca ataaaatccg
     9901 cgacatcatc tcctacgagc cgccgcacac cgtactgtaa tcccgcaaac ggacatcgat
     9961 tatggatatt aaaatcaacg acatcaccct cggcaacaat tcgcctttcg tcctattcgg
    10021 cggcatcaac gttttagaag atttggattc caccctccaa acctgtgcgc attacgtcga
    10081 agttacccgc aaactgggca tcccctatat ctttaaagcc tctttcgaca aggcaaaccg
    10141 ctcgtctatc cattcctatc gcggcgtagg cttggaagaa ggcttaaaga tttttgaaaa
    10201 agtcaaagca gagttcggca tccccgtcat taccgacgta cacgaacccc atcaatgcca
    10261 acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgcc tttcttgcgc ggcagaccga
    10321 tttggtggcc gcaatggcgg aaacgggcaa tgttatcaac atcaaaaaac cccagttcct
    10381 cagcccttcg caaatgaaaa acatcgtgga aaaattccgc gaagccggca acgggaagct
    10441 gattttatgc gaacgcggca gcagcttcgg ctacgacaac ctcgttgtcg atatgctcgg
    10501 tttcggcgtg atgaaacaaa cctgcggcaa cctgccggtt attttcgacg ttacccattc
    10561 cctgcaaacc cgcgatgccg gttctgccgc atccggcggt cgtcgcgcac aggctttgga
    10621 tttggcactt gcaggcatgg caacccgcct tgccggcctg ttcctcgaat cgcaccccga
    10681 tccgaaactg gcaaaatgcg acggccccag cgcgctgccg ctacaccttt tagaaaattt
    10741 tttaatccgc atcaaagcat tggacgattt aatcaaatca caaccgattt taacaatcga
    10801 gtaacacggt ttcgccttat gatgcagact ttccgaaaaa tcagcctgta tgccgcaacc
    10861 ttgtggctcg gtatgcagat tatggcaggt tatatcgccg caccggtgct gttcaaaatg
    10921 ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt tcgacatcct ctcttggagc
    10981 gggcttgccg tttggggcac ggtactggct gccgcctttg ccgccctaac ccggcggcaa
    11041 accgccctgc tgcttttttt attgtccgcc cttgccgcca accaattttt ggttacaccc
    11101 gttatcgagg cactgaaata cgggcatgaa aattggctgt tgtcggttgc aggcggatcc
    11161 ttcggaatgt ggcacggtat ttccagcatg actttcatgg caaccgccct actttcagca
    11221 gttttaagtt ggcggctttc cggcaaagag gccgtctgaa gccctcccat tttttacctc
    11281 ccttcacttc acttggagaa cattcatgag cgcaatcgtt gatattttcg cccgcgaaat
    11341 tttggactcg cgcggcaacc ccacagtcga gtgtgatgta ttgctcgaat ccggcgtaat
    11401 gggacgtgcg gccgtaccga gcggcgcatc caccggtcag aaagaagctt tggaacttcg
    11461 cgacggcgac aaatcccgct attccggcaa aggcgtattg aaggcggtcg aacacgtcaa
    11521 caaccaaatc gcccaagccc tcatcggtat cgatgccaac gagcaatctt atatcgacca
    11581 aatcatgatc gaattggacg gtactgaaaa caaaggcaat ttgggtgcga atgcgacttt
    11641 ggcggtctct atggcggttg cacgcgccgc tgccgaagac tcaggcctgc cgctttaccg
    11701 ctacttgggc ggcgcaggtc cgatgtccct gcccgtaccg atgatgaacg tcatcaacgg
    11761 cggcgaacac gccaacaaca gcctgaacat ccaagagttt atgattatgc ccgtcggcgc
    11821 aaaatctttc cgcgaagcgt tgcgctgcgg tgcggaaatt ttccacgcct tgaaaaaact
    11881 gtgcgacagt aaaggcttcc cgaccacagt cggcgacgaa ggcggtttcg cccccaacct
    11941 gaacagccac aaagaagccc tgcaactgat ggtcgaagcg gccgaagccg ccggctacaa
    12001 ggcgggcgaa gacgtattat tcgcattgga ctgcgcgtcc agcgagttct acaaagacgg
    12061 caaataccac ttggaagccg aaggccgctc ctacaccaac gcggaatttg ccgaatactt
    12121 ggaaggcttg gttaacgaat tcccgattat ttccattgaa gacgggatgg acgaaaacga
    12181 ctgggaaggc tggaaactgc tgaccgaaaa attgggcaaa aaagttcaat tggtcggcga
    12241 cgacttgttc gtaaccaatc cgaaaattct tgccgaaggc atcgaaaaag gcgtagcaaa
    12301 cgcattgctg gtcaaagtca accaaatcgg tactttaagc gaaaccctga aagccgtcga
    12361 tctggcaaaa tgcaaccgct acgccagcgt gatgagccac cgctccggcg aaaccgaaga
    12421 cagtaccatt gccgacttgg cagtcgccac caactgtatg cagattaaaa ccggttcttt
    12481 gagccgttcc gaccgcatgg cgaaatacaa ccaactgctg cgtatcgagg aagaattggc
    12541 ggaagccgcc tactaccccg gcaaagccgc attctaccaa ctgggcaaat aaataaaaaa
    12601 gtggatgtat gtttcaacac acagggcaac acatgaagcg tcgccctgtg tgttgacctg
    12661 atttggaaga ggttacacct ctcccaaata aagtctaatt ttaccgccac gaaggacgga
    12721 catccgagtg gtggggtttc aaccagtaag gaaacgttga tgaagtgggt aactgtcgtt
    12781 ttatccttcg cacttgtctg ttgccaatac agcctgtggt tcggcaaagg cagcatcgga
    12841 cgcaacagca gtctgagaga acagattgcc gttcaagaag aaaaaaacca gacactcgcc
    12901 ctacgcaatc attcccttgc cgccgaagtc tatgatttgg aaaacggtca agaagccatt
    12961 tcggaaatcg cccgggtaga actgggttat atccaagacg gcgaaacctt ttaccgactc
    13021 atcaggcata accggtaata ccgtcaaaaa gccgtccgaa ccaatgttcg gacggctttt
    13081 atttcaacaa actgtcagac agcccctcat cctcccccgc caaaccgcaa tccagcctga
    13141 catccccctc gacgcaacag cagcacggca gtatctcgtc ccgccccaaa aaagccaaag
    13201 gcggctcccg ataagtaacg cttccctcca aaatctttac ccggcacgat ccgcaatatc
    13261 cgcttcggca ctgatattcc accatatgcc ccgtccgttc caagccttcc aacagggtct
    13321 caccctccaa gagctcaaaa aggcccttat tcgtaccaat gcgcgccatt tccgaccaat
    13381 caaaaataaa aggaacaata accgatccta cctgctgttt ttcccatcat acaacacaca
    13441 aatgccgtct gaaacatccg gcttcagacg gcattttttc aaaaaacatt tacaactcaa
    13501 aatcgcccaa gtcatccgta ttcacttcag aatcgatttg accaatcaaa taagaggata
    13561 tttccacttc ctgcggcgcg acctgtacgt tgtcggacga cagccacgca ttaatccacg
    13621 gaatcgggtt ttgatttgcg ccttcaaatc cggccggcag ccccaccgcc tgcatacgca
    13681 gattggtaat atattcgacg tattgggata agatttcttt gttcaaaccg atcatcgaac
    13741 cgtctttaaa caaatatgcc gcccattctt tttcctgttc cgccgctttt ttgaagagtt
    13801 ggaaacattc gtcctgcaac tcggcggcaa tttccgccat ttccggatca tcaacacccg
    13861 aacgcatcag attaagcata tgctgcgtgc cggtcaggtg cagggcttca tcgcgggcaa
    13921 tcagtttgat gattttggcg ttgccttcca tcaactcacg ctcagcaaaa gcaaacgagc
    13981 aggcaaatga aacgtagaaa cggatggctt ccaacacatt gacgcacatc aggcagagat
    14041 agagtttttt cttcaacccg cgcaaagaca cagtaacggg ttttccgccg acattgtgca
    14101 ccccttcgcc caacaggttg taatattggg tgtattcgat taaatcatca taatagcagg
    14161 caatgtcttc ggcgcgggcg gtaatgtatt cgttttcgac aatatcatca aacacgaccg
    14221 acggatcatt cacaatattg cggatgatgt gggtatagct gcgcgagtgg atggtttcgc
    14281 tgaagctcca cgtttcaacc caagtttcca gctcgggaat cgaaaccaaa ggcagcaagg
    14341 caacattcgg actgcgccct tggatggaat cgagcagtgt ttgatatttc agattgctga
    14401 tgaaaatatg tttttcgtgt tcgggcaggt tggcgtagtc gatacggtcg cgcgacacgt
    14461 cgatttcttc cggccgccaa aaaaacgaca attgtttttc aatcagtttt tcaaatacct
    14521 cgtatttctg ctggtcataa cgggcgacat ttaccggctg accgaaaaac atcggctctt
    14581 tcagcgcgtc gtttttggtt ttgggaaagg tgctgtatga catgactagg tgttcgcagg
    14641 acataacgtc tctcttttta aaaattagtc gacttttcta ttaattttta attcaccata
    14701 taaaacatgt tgaattctat caataaagtg ccatcctaa
//
LOCUS       DAWTHD010000060        14542 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWTHD010000060 DAWTHD010000000
VERSION     DAWTHD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14542
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.060"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(124..2595)
                     /locus_tag="WM704_001348"
     CDS             complement(124..2595)
                     /locus_tag="WM704_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFA9340762.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(2592..3770)
                     /gene="hflX"
                     /locus_tag="WM704_001349"
     CDS             complement(2592..3770)
                     /gene="hflX"
                     /locus_tag="WM704_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFA9340763.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTVRRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETVQADVLLHVVDAAARNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(3776..5056)
                     /locus_tag="WM704_001350"
     CDS             complement(3776..5056)
                     /locus_tag="WM704_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFA9340764.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            complement(5208..5933)
                     /locus_tag="WM704_001351"
     CDS             complement(5208..5933)
                     /locus_tag="WM704_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFA9340765.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            complement(6006..6683)
                     /gene="radC"
                     /locus_tag="WM704_001352"
     CDS             complement(6006..6683)
                     /gene="radC"
                     /locus_tag="WM704_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFA9340766.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            complement(6806..8155)
                     /gene="gshA"
                     /locus_tag="WM704_001353"
     CDS             complement(6806..8155)
                     /gene="gshA"
                     /locus_tag="WM704_001353"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFA9340767.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            8407..9816
                     /gene="leuC"
                     /locus_tag="WM704_001354"
     CDS             8407..9816
                     /gene="leuC"
                     /locus_tag="WM704_001354"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFA9340768.1"
                     /translation="MTAQTLYDKLWNSHVVREEGDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            9911..10165
                     /locus_tag="WM704_001355"
     CDS             9911..10165
                     /locus_tag="WM704_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340769.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            10227..10868
                     /gene="leuD"
                     /locus_tag="WM704_001356"
     CDS             10227..10868
                     /gene="leuD"
                     /locus_tag="WM704_001356"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFA9340770.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            11035..12306
                     /locus_tag="WM704_001357"
     CDS             11035..12306
                     /locus_tag="WM704_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9340771.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            12322..13053
                     /locus_tag="WM704_001358"
     CDS             12322..13053
                     /locus_tag="WM704_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFA9340772.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            13087..14157
                     /gene="leuB"
                     /locus_tag="WM704_001359"
     CDS             13087..14157
                     /gene="leuB"
                     /locus_tag="WM704_001359"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFA9340773.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            complement(14185..>14542)
                     /locus_tag="WM704_001360"
     CDS             complement(14185..>14542)
                     /locus_tag="WM704_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9340774.1"
                     /translation="GRAYTVAEDNAEPETLPPAVKKKIMPDGIVYADSPGSRGKSDAG
                     GFTRCRINRSKEFADRRNHINGIGNFWNQAKRALRKYNGIDRKPFPPLLRECEFRFNF
                     GTPSRQLKILRDRCGI"
BASE COUNT         3445 a         3890 c         3816 g         3391 t
ORIGIN      
        1 aattcgatct caaattttaa acgccaacgt gtttgagcgg taacaatgca tactttaagt
       61 ttgtaatcct tcatcgtctc atttttgttt taatcgacta tacgtctaaa gcaacgccgc
      121 ccgtcattgt tcggacagta ttttttcaga cggcattttc ccccgtttgt gaaggcgcaa
      181 ggcattgccc aaaaccgcca gcgaactgaa actcataccc agtgcggcta tccacggttg
      241 gacatagccc aaaacggcaa gcggtacggc gatgatgttg tacgcgcccg cccatatcag
      301 gttttgccgg ataatatggc gggtgcgccg cgcctgatcg agcaggtggg cgacggtacg
      361 caaatcttcg ttcaataaca caatgtccgc gccgtccctc gcaatatccg tcccgcccgc
      421 tgcggcggcg gatacgtctg cctgcgccaa aacgggcgcg tcgttgatgc cgtcgcctat
      481 catcagcact tttttccctt ctttttgcaa ggcttcgacg tattccagct tgtcttccgg
      541 cattgcttgg gcgcggtagt gcgcgatacc caaggcacgg gcggtttcag caactgcttc
      601 ttcacggtcg ccactgagga tgtgcagcgt caggtttttg cctgccaact gccgcactgc
      661 ctccgccgcg ctgtctttga gcgggtcttg caggtagaac acggcttgga aaccgctttg
      721 actgccgagg taaaccgcgc tgccgccgcc ttctgtttgc ggttctttac ctgaaatttc
      781 ggcaacatag gatgccctcc ccaatgccca aacctgtgtt tcgccgttga cggtcagttg
      841 cgcacccacg ccttccccga tgcggttgag gcgttgtccg acttggattt cggggacgct
      901 gccgcctgaa atgcggcagt tgagaatggc gcgcgcgagc gggtgttcgg actgctgttc
      961 caaactttgc gccactgcca gcacttgggc ttcggtcatg ctgccgagca gttcaatccg
     1021 gcggacggcg ggattgcctt gggtcagcgt gccggttttg tcgaaaatga tgtcggtggt
     1081 ttgggagagg gtttcgattg cctgctttcc gccgattaaa ataccttcgc gcgccagcgt
     1141 accggtagaa gctgccagcg cggtcggcgt ggcaagcgac agggcgcagg ggcaggtaat
     1201 gaccagcagg gcgacggtaa tccacaatgc ggtgtgcgca tcggcgtaca gcgtccagcc
     1261 gatgaagacg gggacggcaa gcaggagctc gccgaatatg aaagatgagg cgtattgttc
     1321 cgccaactcg gcagtacgcg gtttttgcgc caaggcgcgg tcgagcaggc ggacgatgtg
     1381 cgacaggcgc gtgccgccgc cggtgcggtc ggtgcgtata atcagagggc tttgcgtgtt
     1441 gagtgtacct gcggttactt tttcagacgg cattttggcg acgggcaggc tctcgccggt
     1501 cagcatagat tcgttgacgg cactgtttcc ttccagcacc gtgccgtcaa cggggatggt
     1561 ttcgccgggt ttgaccatca caatatcgcc tgcttggagt ttgacgacgg cggattcgcg
     1621 gacatcctgt acggcaggat agccgggcat ccggtggcag aaggcgggta tcagtttcac
     1681 cagcctttcg gcggcatcgc ctgccttgcg tcgggcaatg tgttccataa agcgtccgcc
     1741 cagcaggaaa aacagcagca tcgcgatgga ttcgaaatac atcccctgcc ctgcgttggt
     1801 ggcgaggctg taaatgccgg cgatgaaggt catgatgatg gcggcggcaa tcggcgtgtc
     1861 cataccggcg cggcggtttt tcaggtcgcg caacgcgcct tgataaaacg ggacggcgca
     1921 ataaaatacg acgggcagca ccatcaggaa gccgccccaa tggaggatct gcaggaaatc
     1981 gggttcgatg tcgccgccgt aaaggtaggt cggcagcgcg aacatcatcg tctgcatcat
     2041 acccagtccg gcaacggcaa ggcggacgat gtattgtttg cgttcttttt ggttggcggc
     2101 ttcgattttt tgcgcgtcat agggtgcggc ggtgtagcct gtctgccgga ttttcaacag
     2161 aatgtcggag aggcggattt tgccgtcgtc ccagacgacg cggcagcggt gcgtgctgta
     2221 attgaggtcg atgcggacga tgccgtctgt gcgcaaaagc tgctgttcga tcagccagac
     2281 gcaggcggcg caggtaatgc cgctgagcat cagcactgct tcgtgcgtgc cattatgggt
     2341 ttccacaaag tcggattgga cttcgggcag gtcgtacagg cggatttggt cgaggatttc
     2401 ttggggcggc agttcggttt ttttcgcgtc ggcggtgcgt cgtttgtaat aactgcccaa
     2461 gccggaatcg atgatgcttt gtgcgactgc ctgacagccg acgcagcagg tttcgcggtc
     2521 ttcgccttcg tagcggacgg tcagatgcag gttttcggga acgtccagcc cgcagtggaa
     2581 acaggttttt ttcatggcat ttcggtttcg tctgtgtttg gtgcggcggc acaatactcg
     2641 gcaatggctt cgcgcagtgc gtctataccg gtattttcag caacggaaat gcggacggcg
     2701 gcaatttttc ccgcagcgtc gcgccatatg cccgtgtttt gttcttcaga cggcagcagg
     2761 tcggttttgt tgtacacctt gatgcacgga atatcgtggg catggatttc ttgcagtacg
     2821 ttttccacgt cttcaatctg ctgcccgctg ttccgggcgg cagcatcgac gacgtgcagc
     2881 agcacatcgg cttgcacggt ttcttccaag gtggcggaaa aggcggaaat cagtttgtgc
     2941 ggcagatcgc tgacgaatcc gacggtatcg gtcaggataa tgctgcatgc gggactgatg
     3001 tacagccgcc gcaccgtcgt gtcgagagtg gcgaaaagct ggtctttcgc atatatgccc
     3061 gacttggtca gccggttgaa caggctggat ttgccgacat tggtatagcc gaccagcgca
     3121 aacgttttga ttctgcccga ctcgcgggac ttgcggcgca gggcgcgctg ttttttgagg
     3181 ttggcaagct gttttttcaa ggcgttgatc cgatgggcgg ttaatcggcg gtcggtttcc
     3241 agtttggttt cgcccggccc tttcatgccg ataccgccgc gctggctttg caaatgtccg
     3301 taaccgcgta tcaagcgtcc cgccaaatgg ctcaattgcg ccaactcgac ttgcagcctg
     3361 ccttcctgcg tgcgggcgcg gcgggcgaaa atcgccagaa tcagccccac tctgtccaat
     3421 acgcggcatt ggaggatttt ttccaaattg cgttcctgcg tgggagtaag ttcgtggttg
     3481 aatacgacca aatcaatgcc gtctgcggca actgcttccg acagctccgc cgccttgccc
     3541 gtgccgacaa acagcgcagt gtgcgggcgg tcgcgtttgg cagtctccac gcgtacggaa
     3601 tcgccgcccg ccgctttgac cagctcgacg gcttccgcca atgccgtctg aaaaccgttc
     3661 agacgggcgg cattgctgcc cgtatcatct ttatccaaca ttacgcccac cagcatgacg
     3721 cgttccggtt gcgcctgagt ggcactgttc cgtcctgttc tacctgacat aactgtcaat
     3781 cctgcaaata aagggttaca ccgcgttccg ggctgtcttc cggagtaagc ctgatgcggc
     3841 ttcccgccat tccgctgacg ctgaagacaa tgccgcccgc ttcgtgccgg agtatggaca
     3901 ggctgttgcc gaactgccgc cggtaggtat aacgggtaca cgcttccaga caccagcgtc
     3961 cgcgccagtg gatgaaatat ttttcgcctt ttctaaccgc aagtgtctcg ccgccgttta
     4021 cctcaaaata agggtaatcg ggttttcggg ttctaaactg ccatttcgcc tgcgcgcgcc
     4081 gtccgttgcg gacatagtcg aataccgccg tataaagcgt gccgtattcc aaaggtttga
     4141 gcgggaaaag ggcgaattgg tgcgcggtca gcctgccgtt agggtcgttg ccggcggtta
     4201 aaaccctgac ggggcggatt tcgtttttac cctgatacag cttgaaactt ttcatcgcaa
     4261 ttttgcctgc cgcctcggaa aaatcaatgc tggcaggatt gcctgtgatt tcatattccg
     4321 gcacggggtc gggacgttcc ccgtaaaaat aaggcagcgc gccgccgcca accggatagg
     4381 cggtataaag caattccgtt acgggcatgg cttcgtcagc ataaacggcc gcaccgttgt
     4441 ggcaagcgtt gcggtaatat ttccgtcctg cttccggctg ccgccttcct tttgcacagg
     4501 cgcgctcgaa gctgccgttg ccctgattga atacgaggac ggttttgccg ttttcgcgca
     4561 caaatgccgc acctgcttcg tcggtatggc ggtcaagcag cgaaaggcgg tgatagacag
     4621 cactcatcaa agcgtccact tggcgttgct gcgtgcggat gtcgctgtcg gacgattcgg
     4681 cggcttcctc ttccgtgctg atgttttcat gcacgccgtt gtagagatac ccggcaaggc
     4741 gtgtgcgttc ggtcagcttt tgtgcggtgt agtgcggatt gtcgggatgg tgttcgccgt
     4801 gtccgtcttc gggattgagc gtgagatagc gtgcgtgcct gcgggcggaa ttttccaaaa
     4861 ccggcgcgtg tgccagcgcg tgcaaaccga tttgtgtgcg gatgcggttg aggtagtgca
     4921 gcgcatcgcc gccgtccctg atttgcggtg cggacggata gacaagttcg cccgcgggca
     4981 gggattggtt ttgggtatgg tagaaaacgc ctgccgccga gcccaataga agcagccaaa
     5041 taaaaaggga tttcataatg ccgccctccc gacgcggcaa cggggtgttt tatcaaaacg
     5101 gcaaacttgc caatgcggtt tccgcttgcc gattgccaaa ccggcaaacc tttcccgtgc
     5161 cggtgccgaa cccgatgccg tctgaacctt cagacggcat cgggtgttta tttgtccact
     5221 cggacgggtg caggttcgta ttgtgtcgat tcgtcgccgt aatacagcac gccgagtttg
     5281 acggggatgc gtccctgcga tttgcggtgg gcgttggaat cgcgcaagga ataggcgcag
     5341 ccgcagtatt cctgctggta gaagttttcg cgtttgctga tttcaatcat acgcgcgccg
     5401 ccgccgcctt tgcgccagtt gaagtcccaa taggccacat catcgtaagg cgcggcggca
     5461 cggtgtccgc agtcgttgat ttgcgccata tttttccagc gtgaaatgcc cagcgaactg
     5521 gtaaagacgg gaaaaccgtg ttcgtgcgcg tattgcgccg ctttttcaaa acgcatatcg
     5581 aaacacatgg tgcagcggat gccgcgttcc ggctcaaact ccatgccttt ggctttggca
     5641 aaccattctt tgcggtcgtt ttcgtagtcg tcgtctttat cgatgaaagg gatgccgaac
     5701 ttttccgcaa agcgcatgtt ttcctctttt cggagcatat actctttgtg cggatggata
     5761 ttgggattgt aaaaataaat ggtgtagtcg atgccggagg caagcatggc ttccatcact
     5821 tcgccgctgc acggggcgca gcaggaatgc agcaagactt ttttatgtcc gccgggcggg
     5881 acgagtatgg ggcggtcaat gtcggtaacg gtaggtttgt tttgtgtttc cattaactta
     5941 acaatatctg tcgaatacgg tcaaagcggc tattttacca gagccgccga atgtaaaaca
     6001 gggtgtcagg gcatcagccc gagctgcctg aacgaacgga cggtttgcga ggtaacgata
     6061 aaatggtcga gcagcgaaac atcaaccagc gacatggcct gtgccagccg ccttgtgaac
     6121 atgatgtctt cctgcgaagg ttcgggcgag ccgcccggat ggttgtgcgc gataatcagg
     6181 ctgtcggcat attcgtccaa tgccagtttg acgatttcgc ggatgtaaat cgtgttttcc
     6241 gcaaccgtac cgcgcgacag ctctctgacc gcaatcagtt ggttttggcg gttcagcagc
     6301 agcgcgacgc tgacttcgac tttttcctgc cccaaatgaa agcgtaaata atcggcaacg
     6361 gtatcgggat cggataaggt aatttcttcc tgcaattctt cctccaatat ccgccgcccg
     6421 atttccctga ccacggcgaa ctgtgtgaaa cttgccgtcc ccatcccttt gtatgccgac
     6481 agtttgccga cctccgcgct catcagcctc cccaagctgc cgaactcctg caataggtag
     6541 cgcgccaaat cgaccgcgct catcccgcgc gtcccgacgc gtaaaaggat tgccaaaagt
     6601 tcggcatcgc tcaaagccgc cgccccgcgt tccaacagct tttccctggg cctttcgcct
     6661 tccggccatt gcttgatgct catgttttcc cttttttaaa tagtctgaga tgccgtctga
     6721 aacagatgtt cccacacgcc aaatatccga aatgccgtct gaaccggatt tccgcttcag
     6781 acggcatctg tatttcaaaa cagtcttacg ccgtttcttc caactcgatt gaagccgcca
     6841 aaagcgacag acgtgccatt acgccgtaaa cgtagaggcg gttatgttcg ttgtccacgt
     6901 cgcagtcttt ttcagaacgc ggcataccga gcgaatccca ttgttcgaat acgcgtttgc
     6961 aggcttctgg agcttcttgc gaattatcgc cgttaccggt aggaatgctg ttggacagcg
     7021 gcacaaacac cataccgccg gcgttgaggt tttcgtccgc accgcgccct tcgtgtacgc
     7081 ggaaaaagcc gccgatgacg aaacggtcca tcatatagac gacgggttcg cacaccgcgc
     7141 cgttcaaggt ttcataggta taaacccctt cttggacaat cacttcgctg acttccaagc
     7201 cctctttcac cttcgccatt ttgttgcggt ttttgcggtt caagccgcgc acttcgtcgg
     7261 aggatttgac gctcatcaca cccatgccgt aagtgccggc atcggctttg acgatgacga
     7321 aaggtttgtc ggcaatgccc gattcgtcgt atttggcttg aattttcgcc agcacgcgtt
     7381 ctaccgcttc cgccagcgca tcttcacctt cgcgcccttg gaagtccaaa ccgccgattt
     7441 tttcaaaata cgggttgatc tgccattcat cgatgtcaat cagcttggca aattcggcgg
     7501 caacttggtt gtacgcgccg aaatgatttg ttttgcggcg cgtcgtccaa ccgccgtgca
     7561 gcggcggcaa aacggtttgg ccgatgcctt tgagaatgtc gggaatgccg gcggacaaat
     7621 cgttgttcaa caaaaccacg caaggcgaaa agccgtcggc aaggtggacg cggtcgcggg
     7681 ttcgcagcaa aggctcaagc aggattttgt cgcccaatgc agtttcaaat tcggccggtt
     7741 cggttacttc cggattcagg ctgcccaagc gcacttcaca ccctgccgaa cggaaaatct
     7801 cgccgagggc gtaaacgttt tgcaggtaaa acgtattgcg cgtgtggttt tcgggaataa
     7861 tcaatacgga tttctccgtt tcgcaggcgc gttgcaccgc atcttgcgcg gcaaccgccg
     7921 ccagcgggat gaaattcgga ttcagattat tgaagccgcc cggaaacaaa ttcatatcga
     7981 tagacgaaat tttgtaaccg gcgttgcgta tatcgaccga accgtaaaac ggcgggcggt
     8041 gcgcgttcca ctgcgtgcgg aaccacgcct cgattttggc gtgattggac aggatttttg
     8101 cctcaaacgc ctgaagttgc gccgaatgtt cgggcgacat aaccggtaat ttcatctttc
     8161 gtctgcctct gtgtgttttt tgacaaggta cagataataa tgcttttgaa agaatgacga
     8221 caagtattct ttcgtatcga atcattcttt agatataacg aaatgaacaa tatgtctaat
     8281 tttttcgtta aaattgttta caaaataaca ccgactcaaa aattagacaa aatctgttgc
     8341 gcagtataaa gaatacgtct aaaatccgcc caatccgcat caccatttat ccaaaagaac
     8401 aacatcatga cggcacaaac cctctacgac aaactctgga acagccacgt cgtccgcgaa
     8461 gaaggagacg gcaccgtcct gctttacatc gaccgccatc tggttcacga agtaaccagc
     8521 ccgcaggcgt ttgaaggtct gaaaatggcg gggcgcaagc tgtggcgcat cgacagcgtg
     8581 gtctccaccg ccgaccacaa caccccgacc ggcgattggg acaaaggcat ccaagacccg
     8641 atttccaagc tgcaagtcga tacgttggat caaaacatca aagagtttgg cgcactcgcc
     8701 tacttcccgt ttatggacaa aggccagggc atcgtacacg ttatggggcc cgaacaaggc
     8761 gcgaccctgc ccgggatgac cgtcgtctgc ggcgattcgc acacttccac ccacggcgca
     8821 ttcggcgcac tggcacacgg catcggcact tccgaagtcg aacacacgat ggcgacccag
     8881 tgcattaccg cgaaaaaatc caaatctatg ctgattgccg ttgacggcaa attaaaagcg
     8941 ggcgttaccg ccaaagacgt ggcgctctac atcatcgggc aaatcggcac ggcgggcggc
     9001 acgggttacg ccgtcgagtt cggcggcgaa gccatccgca gcctttcgat ggaaggcagg
     9061 atgaccctgt gcaatatggc gattgaggca ggcgcgcgtt ccggtatggt tgccgtcgat
     9121 caaaccacca tcgactatgt caaaggcaag cccttcgcac ccgaaggcga agcttgggac
     9181 aaagccgtcg agtactggcg cacgctggtg tctgacgaag gcgcggtttt tgataaagaa
     9241 taccgtttca acgccgaaga catcgaaccg caagttactt ggggcacgtc gcccgaaatg
     9301 gttttaaaca tcggcggcaa agtgccgaat cccgccgaag aaaccgatcc ggtcaaacgc
     9361 agcggcatag agcgcgcact cgaatatatg ggtttgaagg ccggtacgcc gctgaacgaa
     9421 atccctgtcg atatcgtctt tatcggctct tgcaccaaca gccgcatcga agacttgcgc
     9481 gaagccgctg ctatcgccaa aggccataaa aaagccggca acgtacagcg cgtgttaatc
     9541 gtccccggct ccggtttggt taaagaacaa gccgaaaaag aaggcttgga caaaattttc
     9601 atcgaagccg gttttgaatg gcgcgaaccg ggctgttcga tgtgccttgc gatgaatgcc
     9661 gaccgcctcg cgccgaggca acgctgcgcc tccacgtcca accgcaattt tgaaggccgc
     9721 caaggcaacg gcgggcgcac ccacctcgtc agccccgcta tggccgccgc cgccgccgtt
     9781 accggacact ttaccgacat ccgcacgatg gcgtaagtat tttcaggcag tctttaagaa
     9841 tttgacgagg ccgtctgaaa tatgtcccgt aaggcagaaa cacctaatat tcaaaccaag
     9901 gagtttaacc atgaacaaac ttttcgttac cgccctgtcc gccctcgcct tgtccgcctg
     9961 cgccggcact tggcagggcg cgaaacaaga caccgcccgc aaccttgaca aaacacaggc
    10021 cgccgccgaa cgcgccgccg aacaaacagg caacgccgtc gaaaaaggtt gggacaaaac
    10081 caaagaagcc gtcaaaaaag gcggcaatgc cgtcggacgc ggcatttccc atctcggcaa
    10141 aaaaatcgaa aacgccaccg aataaccgat ttgcgatgcc gtctgaaacc gtttcagacg
    10201 gcatcgtcag cacaagacgc aaacccatga aagcctttac caaaatcacc gccatcgtcg
    10261 ccccgctcga ccgcagcaac gtcgataccg atgccatcat ccccaaacaa tttctgaaat
    10321 ccatcaaacg cagcggcttc ggccccaacg cctttgacga atggcgttac ctcgaccacg
    10381 gcgaaccggg catggacaac ggcaaacgcc cgttgaaccc cgatttttcc ctgaaccagc
    10441 cgcgttacca aggcgcgcaa atcctgttga cgcgtaaaaa cttcggttgc ggctcttcac
    10501 gcgaacatgc cccttgggca ttggacgact acggcttccg cgccattatc gcccccagct
    10561 tcgccgacat cttctttaac aactgctaca aaaacggcct tttgcccatc gtgttgaccg
    10621 aagaacaagt cgaccggctt ttcaaagaag tcgaagccaa cgaaggctat cggctctcca
    10681 tcgaccttgc cgagcaaacc ctgaccaccc cgggcggcga aacattcaca ttcgacatta
    10741 ccgaacaccg caaacactgc ctcttaaacg gcttggacga aatcggactg accctgcaac
    10801 acgccgacaa aattaaagcc tttgaagaaa aacgccgcca aagccagcct tggctgttta
    10861 acggttaaaa cctatgccgt ctgaaggctg ttcagacggc attcctgata acatataatt
    10921 gctgcggcta atgtaaacag aggggcgata aattgttttc atcttttaag cgggcttagc
    10981 aacactctaa attaatgcaa ctctccattt caaataaatt tttagatttt taatatgcaa
    11041 caaattaaat ttattgactt attttccgga atgagtggca tcaggaaagg attcgaacaa
    11101 gcctgtcgga aacagtcggt tgcttgcgaa tgtgttttta cttcagaaat caaaccggca
    11161 gctttggaag tattgaagca aaactaccct gatgaagtgc cgtatggaga tataacgaaa
    11221 attgaaacag gggatattcc cgattttgac atcctgttgg caggcttccc ttgtcaggct
    11281 ttttctttcg ccggaaaaag attaggcttt gaagatacac ggggaacgct tttctttgat
    11341 gtggcaagga ttttaaaggc aaaaaaacca aaaggtttta ttttggaaaa tgtggaagga
    11401 ttggtgacac acgatagaaa agattcgaca caaaaaatag ggcgcaccct gaccgttatt
    11461 ttggaaacct tggaagcatt gggctactat gtttcttgga aagttttaaa tgcaaaagat
    11521 ttcggcattc cccaaaacag gaagcgcatt tatctgacag gcagtttgaa atccaaaccc
    11581 gacttatctt tcgaaacaag cccaagtccg aaattaaaaa atattttgga atcgggactg
    11641 cctactgaaa gcagcccttt catcaaaaaa ttgctaaaaa aatttccccc gtccgaactg
    11701 tacggaaaat cagtaaaaga caaacgggga gggaaaaaca atattcacag ttgggatatt
    11761 gaattaaaag gcgcagtaac cgaagaagag aagcaattgt taaatatcct tctaaaagaa
    11821 cggaggaaaa aaaaatgggc ttcagaaatc ggcatagatt ggatggatgg gatgcctttg
    11881 acaaaagcgc aaatttcaac tttctataaa caccccgatc tccaaaatat tttggacagc
    11941 ctgacagaca aaggctattt ggttttagag catccgaaac aaaaaattgg cgggcaaaga
    12001 atcaaagatg aatccctgcc caaaggctac aatattgttt caggtaaaaa atcttttgaa
    12061 atcaataaaa tattagatcc aaacgatgtt gcgccaacct tggttgcaat ggatatggag
    12121 caccttttcg tcgttgacaa cggcggtttg cgtacactta ccggaaaaga agggttacgc
    12181 ttattcggct atccggacga ttattcgttt gatattccca aaaaagacag atgcgattta
    12241 ttgggtaata ccgttgccgt ccctgtgatt aaggcggtat ctgaaagact tctgcatact
    12301 ttataaaccg acagggataa aatgataaaa cttactgcac aacaaatatt tgataagttg
    12361 ctggatgaag aaaaaatctt atcggccaat ggtcaaatta gatttttctt aggagatgtg
    12421 gatattatcg tcaaacaaaa agatgttgtc ggcaacatca ttcaggaatg gctcggcgga
    12481 tggttgagaa aaagggaaat tgaatttgat gtttcaacca atacccaaat gcctcccgat
    12541 ttctttttaa ataaaaaaga ccgcagcaga gaattgttgg aagtaaaggc gttcaaccga
    12601 aatgcctgcc cgggttttga tattgcagat tttaaaatgt attctgatga aatcattcat
    12661 aagccctata tgctggatgt agattattta atattcggtt acgatatgga cgacaacggc
    12721 aatgtaacca tcaaggattt atggcttaaa aaagtatggc aaattaccag aagcatggat
    12781 gggtgggcaa ttaatcttca agtcaaaaaa ggcgtggtgc ataaaatccg cccgggtgtt
    12841 tggtacagca taaataaaaa gaatatgcct atgtttgaat gcttggaaga ttttgtttcc
    12901 gcaattgaag aaaccgttta tcaaaacccg gcaacacggc ataacgcatc cttatggaaa
    12961 aagaaatttg aggaagctta taaaaaacac tacaaccgat cgatttccat accccgttgg
    13021 cacgaaattg cacacaaata caaaaagaaa taacccttca accatcaacc cataaggaaa
    13081 cttgaaatga ccaaacatat cgccatcctc cgaggcgacg gcatcggtcc cgaaatcgtc
    13141 gccgaaaccg tccgcgtact cgacaaattt atcgcccaag gcttggatgc cgactacgaa
    13201 tacgcgcctt tgggcggcga agcctacgac gaatacggcc atccttatcc agaatttacg
    13261 caaaacctct gccgcaaagc cgatgccgtc ctgcttggtg cagtcggttc gccccaatac
    13321 gacaatctcg accgtccgtt gcgccccgag cgcggcttgt tagcaattcg taaggatttg
    13381 aacctgtttg ccaatttgcg tcctgccgtt ttatatcccg aattagccaa cgcctccacg
    13441 ctcaagcctg aaatcgttgc cggtttggat attttgattg tgcgcgaact taccggcgat
    13501 atttatttcg gcgaaccgcg cggcatccgt gttttggaaa acggcgaaca cgaaggctac
    13561 aacaccatga aatacagcga aagcgaaatc cgccgcatcg cccacgtggc cttccaatcc
    13621 gcccaaaaac gcagcaaaaa agtctgctcc gtaggcaaag ccaacgtttt ggaaaccacc
    13681 gaactgtggc gcgaaatctt tgaagaaatc ggcaaacaat atcccgatgt cgagctttcc
    13741 catatgtatg tggacaatgc cgccatgcaa ctcgtgcgcg cgcccaaaca gtttgacgtg
    13801 attgccaccg gcaacatctt cggcgacatc ctctccgacg aagcctccat gctgaccggc
    13861 tccatcggta tgctgccttc cgcttctttg gacgaaaacg gcaaaggcct gtacgaaccg
    13921 tctcacggct ccgcccccga catcgccgga caaaacaaag ccaacccgct ggccaccatc
    13981 ctctcgcttg ccatgttgct gcgttacagc ctgaacgacg aagcgcgcgc gcaacaagtc
    14041 gaaaacgccg tccaaaaagt gctgcaacaa ggcttgcgta ccggcgatat ttacgaagaa
    14101 ggcacaaaac tcgtttcctg ctccgaaatg ggcgacgcgg tactcgccgc cttgtaaaaa
    14161 acaaggggct gtactagatt atccctaaat tccacaccga tcccgcagga tttttagctg
    14221 ccgggacggt gtgccgaagt taaatcgaaa ttcgcattcc ctcaacaacg gcgggaaagg
    14281 tttacgatcg attccgttgt attttcgcaa ggcgcgtttt gcctgattcc aaaagttccc
    14341 aatgccgtta atgtggttcc gacggtctgc aaattccttg gaacggttga tgcggcaacg
    14401 ggtaaaaccg cccgcgtccg acttgccgcg gctgcccggg ctatcggcat aaacaatacc
    14461 gtccggcatg attttctttt tgacggcagg gggcaacgtt tcaggctcgg cattgtcctc
    14521 ggcaacggta taggcccgcc cg
//
LOCUS       DAWTHD010000061        14457 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWTHD010000061 DAWTHD010000000
VERSION     DAWTHD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14457
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.061"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            199..1413
                     /locus_tag="WM704_001361"
     CDS             199..1413
                     /locus_tag="WM704_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFA9340775.1"
                     /translation="MAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINLVPFILTAPAP
                     YQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHLSYYDRGRMAN
                     IFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAAMRLSEPKSQKILF
                     IVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEMRELCAYGGLR
                     GFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAGFQKIKTAENL
                     IGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESDIFNHRLKCTE
                     YGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVGNLNETFRYLK
                     QGHVAWLHFKIK"
     gene            complement(1866..2612)
                     /locus_tag="WM704_001362"
     CDS             complement(1866..2612)
                     /locus_tag="WM704_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9340776.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(2791..3462)
                     /locus_tag="WM704_001363"
     CDS             complement(2791..3462)
                     /locus_tag="WM704_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFA9340777.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(3531..4202)
                     /gene="rpiA"
                     /locus_tag="WM704_001364"
     CDS             complement(3531..4202)
                     /gene="rpiA"
                     /locus_tag="WM704_001364"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFA9340778.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(4279..4761)
                     /locus_tag="WM704_001365"
     CDS             complement(4279..4761)
                     /locus_tag="WM704_001365"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFA9340779.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(4793..5482)
                     /locus_tag="WM704_001366"
     CDS             complement(4793..5482)
                     /locus_tag="WM704_001366"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFA9340780.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(5479..6213)
                     /gene="dnaQ"
                     /locus_tag="WM704_001367"
     CDS             complement(5479..6213)
                     /gene="dnaQ"
                     /locus_tag="WM704_001367"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFA9340781.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(6369..7679)
                     /locus_tag="WM704_001368"
     CDS             complement(6369..7679)
                     /locus_tag="WM704_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9340782.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(7669..7854)
                     /gene="ccoS"
                     /locus_tag="WM704_001369"
     CDS             complement(7669..7854)
                     /gene="ccoS"
                     /locus_tag="WM704_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFA9340783.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(7854..8153)
                     /locus_tag="WM704_001370"
     CDS             complement(7854..8153)
                     /locus_tag="WM704_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340784.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            8504..9700
                     /locus_tag="WM704_001371"
     CDS             8504..9700
                     /locus_tag="WM704_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFA9340785.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(9750..9872)
                     /locus_tag="WM704_001372"
                     /pseudo
     CDS             complement(9750..9872)
                     /locus_tag="WM704_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10214..12055)
                     /locus_tag="WM704_001373"
     CDS             complement(10214..12055)
                     /locus_tag="WM704_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFA9340786.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
     gene            12146..12616
                     /locus_tag="WM704_001374"
     CDS             12146..12616
                     /locus_tag="WM704_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFA9340787.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(12689..13501)
                     /gene="hpnC"
                     /locus_tag="WM704_001375"
     CDS             complement(12689..13501)
                     /gene="hpnC"
                     /locus_tag="WM704_001375"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFA9340788.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(13588..14070)
                     /locus_tag="WM704_001376"
     CDS             complement(13588..14070)
                     /locus_tag="WM704_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9340789.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
BASE COUNT         3525 a         4330 c         3416 g         3186 t
ORIGIN      
        1 attcacgccc tgctctccga acaatggacg ctgccgccat tcctgccgaa acggctgctg
       61 ctgtccctgc tgatactgct ggcccccaat gcggtgtttt gggttttggc actgctgacc
      121 gccaccgccc gcccgattgt caatttggac taccttcccg ccgcgttgct gatcgccctg
      181 ccttggcgtt tcgtcaaaat tgccggcgta ttggcgtttt ggccggcggt tttgtttgac
      241 ggactgatga tggtcatcca gctcttccct tttatggacc tcatcggcgc catcaacctc
      301 gtccccttca tcctgaccgc ccccgcccct tatcagataa tgaccgggct gttgctgctg
      361 tatatgctgg cgatgccgtt tgtgttgcaa aaagccgccg tcaaaaccga cttccgacac
      421 attgccgtct gtgccgccgt tgtggcggca gccggctatt tcaccggcca tttgagttac
      481 tacgaccggg ggcggatggc caatatcttc ggcgcaaaca acttctatta cgccaaaagt
      541 caggcgatgc tctacaccgt cagccagaat gccgacttta ttaccgccgg cctggtcgac
      601 cccgtcttcc tccccttggg caatcagcag cgtgccgcca tgcggctgag tgagccgaaa
      661 tctcaaaaaa tcctctttat cgtcgccgaa tcttgggggc tgccgggcaa tcccgagctt
      721 caaaacgcca cttttgccaa actgctggcg caaaaagacc gtttttcggt ttgggaaagc
      781 ggcagttttc ccttcatcgg cgcgacggtc gaaggcgaaa tgcgcgaatt gtgcgcctac
      841 ggcggtttgc gcgggttcgc actgcgccgc gcgcccgacg aaaaatttgc ccgctgcctc
      901 cccaaccgtt tgaaacaaga aggttacgcc acctttgcga tgcacggcgc gggtagttcg
      961 ctttacgacc gcttcagctg gtatccgagg gcgggctttc aaaaaatcaa aaccgccgaa
     1021 aacctgatcg gtaaaaaaac ctgcgccatt ttcggcggcg tgtgcgacag cgagctgttc
     1081 ggcgaagtgt cggcattttt caaaaaacac gacaagggac tgttttactg gatgacgctg
     1141 accagccacg ccgactatcc cgaatccgac attttcaacc acaggctcaa atgcaccgaa
     1201 tacggcctgc ccgccgaaac cgacctctgc cgcaatttca gcctgcacac ccaattcttc
     1261 gaccaactgg cggatttgat ccgacgcccc gaaatgaaag gcacggaagt catcatcgtc
     1321 ggcgaccatc cgccgcccgt cggcaacctc aatgaaacct tccgctacct caaacaggga
     1381 cacgtcgcct ggctgcactt caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca
     1441 gccttcagac ggcattttgc agacagaccg acctcctcaa gcccactttt ttcatcatct
     1501 ccgataaatt gctttgtaag aatccaaaaa tccaaaatca caggaagtta tcaaaaaaac
     1561 agaaaccatc cgccgtcgtt cccgcgaaag cgggaatcca gacccggaaa ccgcagaaat
     1621 ttatcggaaa aaccgaaact cgaaaacaac ggacaaaatt cccgcctgcg caggaatgac
     1681 ggctcataca tcacccaaaa aaaattgaaa ccaaacagct gggattccct cttgcgtgag
     1741 gctgacagat gccgtctgaa agactttcag acggcatagg gcgttttctc tttgaattta
     1801 aattttgcca caaaaaagcc gccccaaatg aatacccggg cagctttttg ttgatatgac
     1861 tccaatcagc ggtgttgcgg attgtaacgt ttttccaaac gcaggaatat ccagcctaag
     1921 aaagtcgtca tcaacagata aatcagggcg gcggtgtaaa gcggttcttc ataaaccgaa
     1981 taccggcccg taatcgtatt ctgaacatac gccaactccg ccacagcaat gaccgacagc
     2041 agcgagctgt ctttcaagag cgtgatgaac tcgctcgcca aaggcggcag catacggcgc
     2101 aatgcctgcg gcagaatcac atagcgcatc gcctgcggat aggtcagtcc caaagaacac
     2161 gccgcctcca tctgtccttt gtctatagac tggatgcccg cgcggaaaat ctcacagata
     2221 tacgcccccg agttggcgat cagtgccaaa gaaccggcaa tcagcggccc gtatccgcga
     2281 cgcagcgcga ttgccgcctc gccgctgacc aaaatgccgt ctgaaggatg gacgaaaaac
     2341 ggaaaccaca catacgccca aatcacaatc tgcacaaaca gcggcgtacc ccggaacagg
     2401 gtaacgtaca gcagcgaaac cttacgcaac gcccacgcca gcacgcgcat cggcgcaccg
     2461 gctttttcca agtgaatcag gcgcgccaac gccaacaaca gacccaatac cgaaccgccc
     2521 gccgtcgcca cgaccgtcag ccccaaggtc gtcagtgcgc cgtaaagaaa catccagcgg
     2581 tattcgtaaa taatgtcaaa acgaaaatcc ataaaccgtc cgtatcaaaa accggcggaa
     2641 ctgccgccgt tgcaaaataa tccgccattt taccgtaaaa accgccgcct gaactttttt
     2701 atcgcggcag acggcggttg cgcgtctccg caaaaatgca gggcgcgcgg ttttcagacg
     2761 gcatttgccg ttcaaggccg tgcggtgtct ttaccaaatg cccaaccatt cgcccacgga
     2821 atccatccaa tccttattgc ccccgccgct cctgcctgcc cggcggtacg cccacggcgc
     2881 ttgcggattt ttagctttcc acaatccttt gcgttccctt tccgcctgaa tttgagcgtc
     2941 ggcatagtcg gcaaaatccg ccttatcctg ctgttcttta gcataacttt tataatgcca
     3001 cgccgccccg tcctgcacct gcatcaggtt caaatcggtt ttgccggcgg atacctgcgc
     3061 cacttcgcgc tgatagcggt cggtttcaaa cacacgtaca ctgactttcc taccctccgc
     3121 cgccgcgcgc aggttgtcgc gcgaacgtgt accgtaagcc tgtttcatct ccggtgcgtc
     3181 gatatacgcc atccgaattt tatgtttcgc gccgtcgccg tcgatgacgt gaagggtatc
     3241 gccgtcatag actttggaca ccgtgcctgt gtagctgtgg ccggatttcg ccgatgcccg
     3301 tcggcgaacg ggcgcgtcgg aacccacgtc ccctgccgtg ccgagtacgt cgagtacggc
     3361 aaccgccgtc cgcaccgcct cactgtcata tcccgtataa cccaacgcgc ccaaaagcga
     3421 cagggcgacg ggaagccatt tcatgatttt tttaatctgc atatttttca aatgccgatg
     3481 ccgtctgaac atctctgaac cggatttcag acggcatctt aacgtcagga ttacccttgg
     3541 cagggataga tgactttcgc accctcttcc gtccccaaaa tcaacacatc ggcggcatcg
     3601 cgggcgaata tgccgttttc gagtacgccg gtgattttgt tgatttcatc ttccatcgtc
     3661 agcggctgat cgatattcaa gccgtggaca tcgacgattt ggttgccgta aaacgtggtg
     3721 tagccgatac gcagttcggg ctgtccgccc atggcaagca gtttgcgcga aacaagggag
     3781 cgcgcgcttt cgacggcttc cacgggcagt gggaatttgc ccagccggga aacatatttg
     3841 ctttcgtccg caatgcagac gaatttttct gatgcgctgg cgacgatttt ttcgttgagg
     3901 tgcgcgccgc cgccgccctt aatcatttgc agggcgtggt tcacttcgtc cgcaccgtcg
     3961 atatagaccg ccaaaccgga cacttcgttc agggaaacga cgggaatgtc gtaccgggca
     4021 agcagttcgc cggatttttt ggaggttgaa accgcgcctt tgattttttt accgcttttg
     4081 cccaaggctt cgatgaagaa attgatggtc gaaccggtac cgatgccgat gtattcgttt
     4141 tcgggtacga attcgactgc tttttcggcg gcgatgcgct tgagttcgtc ttgtgtcgtc
     4201 atatttttat cctttgggaa accgtatcaa caaacagccg ccatcttaac atttttttgc
     4261 acgtcctgcc cgccgcgttc aaatccgtac cagcaatacc gccgcctgcg cctctatgcc
     4321 ttccatccgc ccgagatagc cgagtttttc gttggttttg cctttgatat tgacgcacga
     4381 aatatcgata cccaaatcgg cggcgatgtt ggcacgcatt tgcggaatgt gcggtgcgag
     4441 tttgggtttc tgtgcaatca cggtcgtatc gacattgacc gcctgccaac cctgcgcctg
     4501 aacgctttga tacgccgcac gcaaaaggac gcggctgtcc gcatctttga actctgcggc
     4561 ggtgtcggga aaatggctgc cgatgtcgcc caaacctgcc gcaccgagca gcgcgtcggt
     4621 aacggcgtgc agcagcgcgt cggcatcgga atgcccgagc agcccttttt caaatgggat
     4681 ttcaactccg ccaagtatca gctttctgcc ttcgatcaat tggtggacat cgtagccctg
     4741 tccgatacgg atattcgtca tcgtttgtgt tcctgatgtt ttgaattgaa tttcagacgg
     4801 cattgagcag cagcctgacg atgtatgcgt cctgcggctg cgtcagtttc aaattgcgcg
     4861 cgtcgccctg tatcagtagc ggacgcacac ccagtttttc cacggcggac gcttcatcgg
     4921 taatgccgtc caagttttcc gcagccaatg cgcggtgcag caaacccgct tgaaaaagct
     4981 gcggcgtttg cgcctgccaa agccccgaac ggtcgacagt tgcactgatt tgtccgcttt
     5041 ctgcgcgctt gagcgtatcg gcaacgggaa ctgccaaaat cccgccttcg gcggcgttgc
     5101 ccgcctgttc tatcaaccgc gtcagagctt cagacggcag gcagcagcgg gcggcatcgt
     5161 gtaccagaat attgtcggtt tccgccgcca aaccggtttc caacagtttt gccacaccgt
     5221 tgcggacagt ttcggcgcgg gtctgtccac cgtttttcca cacccgaacc tgtggaaatg
     5281 ccgtctgaac cttatcggca aacgtgtctt cgggcgagac gacaacgacg gtcaaatcga
     5341 cggcctcatg ccgttcaaaa atcccaagta catgttctaa aacggttttg cttccgattt
     5401 cgacatattg cttgggtttg tccgcaccga aacgcacccc gatgccggcg gcgggaatca
     5461 gcgcgatatt tttgcgtttc atgcgttttc cccgccgttt tcagacggca cggcttcctt
     5521 acgccaaata caggcttcgc ccaagccgtc aagatattct tcgtgcgccg ccagttcggc
     5581 ttcatccgcc ttaatcactt tcagtttggc accgagtttc atttcggcag atgcaacagg
     5641 tttggctgtc ggttcttcct cctcggtctc ccccatcaag tcgaattgcc gccgcgtcat
     5701 cgccagatag acttcgccca aaagttcgca gtcgatcaat gcgccgtgca ggacgcgctt
     5761 gctgcggtcg acggaaaaac ggttgcacag ggcatccaga ctggctttct gcccggggaa
     5821 catttcgcgc gccatcgcca gggtatcggt aacggtgcag ccgagttcct cgacggtcgg
     5881 cagccccatc cggcggaact ccatattgag gaagcccaca tcgaatttgg cattgtggat
     5941 aatcagttcc gcaccgcgca gaaaatcggc aatctgcctg ccgacctctg caaacggcgg
     6001 cgcgtttttc tcttccaaaa cctgtatcgt caagccgtgg acgcgtgccg cctcttcggg
     6061 catatcgcgc tcggggtgga catagaggtg caggtttttg tcggtcattt ggcggttgac
     6121 catttccaga ccggcaaact cgaccagacg gtcgccgccg tcggcataca ggccggtggt
     6181 ttcggtatcg aggatgattt ggcgtgtcat catatcggtg tctttcttct gtcttcgtaa
     6241 attgattatt ttttaagcaa tgtatttttc tgttttcatt tcaatgcaca aacccactta
     6301 ttcacagtgt gttcacaaca ttgggcaggc ggattgtgta ttttggggac aattttttca
     6361 gacggcattc aaggtttttt cctgattgcc gccgcgccta aaaaccgcct ttcgcgctta
     6421 atcaaaaata ccgccaacgg aatattgccc aaggcgacaa tcagatacag cagggaaatg
     6481 ctgtcaaaca aaaacagcaa taccgcgctc aaaacggcgg cggaaaccat aaagatgccg
     6541 ttaacgatat tgttggcggc aacggcgcgg gcgcggaaag tctcgctgct ggcggtttgc
     6601 agccaggtat agagcggaac ggagaaaaat ccgccgaaaa agccgatcag cgtcatcacc
     6661 gccatcacgg agtatgccca tccttgcgat aaaaaccaaa aaatgccgtt cagcccttca
     6721 aaacggtgtc cgtgcgtcag ccacaccaaa accaggccgc aaaccgtcaa acccaacgca
     6781 ccaaccgtta cccaagccaa catcagccgt tccctgccga acttggcaca cagtaccgaa
     6841 ccggcggcaa taccgatgga aaacaaagca agcatcaggt taaaaacatt atcgttgccg
     6901 cccaaatgga tttgggtaaa ggtcggcagt tgcgtggtat aaaccgcgcc gacaaaccaa
     6961 aaccacgaga tgccgataat ggcggtaaaa acgggattgt gccgcaccgt ttcacgcagc
     7021 agggattttg taccacggac aatattccac tcgatttggg tatcggcagc cttggcgggt
     7081 acggacggca taaacaggct gccggccgtt cctccgacgg cgaccagcaa aaccagtatc
     7141 ccgacaatat aaggcggcgc gccggcaacc gccgttccca aaatctgacc aaacaggatg
     7201 gcgacaaacg taccggattc aatcaggctg ttgcccatca tcagctcgtt gtcgtcgaga
     7261 taatcgggca gaatggcata tttaagcggg ccgaacagcg tcgattgcgc gcccatgcaa
     7321 aacaggcaga gcaagagcag cggggctgac cggatataaa acccgtatgc cgccaccgcc
     7381 ataatgatga tttccagcac cttgacccaa cgcgccaaaa cggccttgtc gaatttgtta
     7441 cccaactgcc ccgacagcgc ggaaaacagg aaatacggca aaataaacag cagtgcgccc
     7501 aagttcaaca tctgtccggc aggcagaacg ccgttttgcc ccaaaccgta aaacccgatc
     7561 atcacaaaca gcgcggtttt gaacacattg tcattgaacg cgccgagaaa ctgcgtagcg
     7621 aaaagaggcc cgaaacggcg gcttttaacc agtcccaaac cgcctttttt agcgtccatc
     7681 gttttccttc tctttttcaa tcagtttact tgtcgaatca tcatccatca aaatgcggtg
     7741 cgccggtcct tccaaatcgt caaactgtcc gtttttgccc gaccaccaga aaaaccagcc
     7801 gatgacaaat gccaaaataa tgctgatggg caccagaata aacatacttt ccatcacata
     7861 ttccctatca aatcgttcaa aacaaaagtc tgccctgaca cggtcagata ttcgttacgc
     7921 aaagttccga cgggggcttc gtcaaaaaac agctcgatac ggtctttgac cacgcgccaa
     7981 tattggggaa tttccgtctg accgaacggc gacaggacat ggttgcccgt cccatcttcc
     8041 aatttaacgg caaaacgccc gctctgcagc cgtgcttccg attcgtcgtc ggcaagaagg
     8101 atgaaaaagc ctatatgccg tcccgattgg tcatgaatac tgaaataatg cataaattcc
     8161 ccacccgcct tttttcagac ggcatcaact aaaaacaggg cgaatgtacc agtttggacg
     8221 ggaaaaatgc aaagaaattc tccctccccc agccgaaaac accggcaaac cgcatatccc
     8281 ccttttttcc gtcaaaatac ccgacttccg ccattttcac gcaaacgccc gattaagcca
     8341 agcaattgca aagatttttt gctagaatag cctgcttctt ttatcaaccg tttcagacgg
     8401 ctccactact ttcccgtcaa ggaaagcaaa gcggattcgg cacgaatccg attagtatcc
     8461 gtgtccgatt ccaatgccgt ctgaaacttt ccggagtaag aaaatgtccc aaaaattgat
     8521 cttggttttg aactgcggca gctcgtccct caaaggcgcg gtcctggata acggcagcgg
     8581 cgaagtcctg ctgagctgcc ttgccgaaaa actcaacctg cccgatgcct acattacatt
     8641 caaagtaaac ggcgaaaaac acaaagtcga tctgtccgca caccccgacc acaccggcgc
     8701 ggtcgaagcc ctgatggaag aactcgaagc ccacggcctc gacagccgca tcggcgccat
     8761 cggccaccgc gtcgtcagcg gcggcgaact gtacaacgaa tccatcctcg ttgacgacga
     8821 agtcatcgcc ggcatcgaaa aatgcatccc gctggctcct ctgcacaatc ccgcccacct
     8881 cttgggcctg cgtgccgcgc aaagcatttt caaaggcctg cccaacgtcg tcgtattcga
     8941 tacctccttc caccaaacca tgcccgaagt cgcctacaaa tacgccgttc cgcaggagtt
     9001 gtatgaaaaa tacggcctgc gccgctacgg cgcgcacggt accagctacc gcttcgttgc
     9061 cgacgaaacc gcgcacttcc tcggcaaaga caaaaaagac ctgcgtatgg tcattgccca
     9121 cttgggcaac ggcgcatcca tcaccgccgt cgccaacgga gaatcgcgcg acaccagtat
     9181 gggcctgacc ccgctggaag ggctggtaat gggtacgcgc agcggcgaca tcgatccttc
     9241 cgtattcggc ttcctcgccg aaaacgccaa tatgaccatt gcccaaatca ccgacatgct
     9301 gaacaaaaaa tccggcctgc tcggcatttc cggcctgtcc aacgactgcc gcaccattga
     9361 agaagaagcc gccaaggggc ataaaggcgc gaaattggcc ttggatatgt ttatctaccg
     9421 ccttgccaaa tacatcggca gtatggcggt tgccgcaggc ggcttggacg cactggtctt
     9481 taccggcggt atcggcgaaa actccgacat catccgcgaa cgcgtaatcg gctacttggg
     9541 cttcctcggt ctgaacatcg accaagaagc caacctgaaa gcccgcttcg gcaacgccgg
     9601 cgtgattacc actgccgaca gcaaagccgt tgccgtggtc attccgacca acgaagagct
     9661 gatgattgcc cacgataccg cccgtttgag cggtctgtaa ggttttatcc tcacgcgaac
     9721 tgcctccgga aacggaggca gtttttttat agtggattaa atttaaaccg gtacagcgtt
     9781 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     9841 ttaatccact atatccggct ttccatgctt aaacagcact acctcttttc agacattgac
     9901 ggttgcagcc gcctacctga accttaatga ttgactattt tttaatcatg ttattatttt
     9961 ccataaaata catggcatta agatgttttt ccacaaaaga tacacacacc ggcaaacacc
    10021 ggctgtgttt atcttttctt atgcctattt tttaatcatc gtatttttat cttttaattt
    10081 caatacgcaa accgacttat acacacggtt ttcacatctt tggactgctt ccgtgtgtat
    10141 agcggatatt gccgttttcc tgtctgacaa aaatgccgtc tgagaacttc agacggcatt
    10201 tgaaacatcg gaatcagcgg ttttgttcat accactcgat aaacttgtct gctttgacaa
    10261 aacccagcag cggctcgctg cggctgccgt cggcgcggac gacaaacacg cccggcggcc
    10321 cgaacagacc gtattctttc aacaacgcct gatgttcggg cgtgttggcg gttacgtcga
    10381 tctggaaaaa gcgttccata tcgactgcct gatgcacttc cggctgattg agcgtgtaaa
    10441 ccgccatttc tttgcaggaa atgcaccagt cggcataaaa atccaaaacg acaggtttgt
    10501 cgggatgttc tttcaacgcc gtatccatcg ctgccttcag cgcggcagta tcggcaaaca
    10561 ttttgccgtg ttccgaaaat ttgcctgctt cggccggcgg attgagggtc aagaaatggt
    10621 gcagcgcggt cgttttgccg tttgcgccct gccagccgaa ccacgcgccg cctatcagca
    10681 atatgctgcc caatgcgaat gccacagctt tcggacggcg tttctgcctg cgtccgttga
    10741 ccagcagcat aaaggcagga accagcatca gcagcgtgta cagcgcgacg acgagataat
    10801 agggcaaatg cggcgtggcg aggtaaacgg cgacggcaag caggataaag ccgaaagcgt
    10861 atttgacggc attcatccaa tcgcccgcct taggcaggat atgtcctccg aacgtgccga
    10921 tggcaatcag cggcacgccg gtgcccaacg ccaaagtgta aagtgccaaa ccgcccaaaa
    10981 ccgcatcgcc cgtctgaccg atatagccca aggcaaacgc cagcggcggg gcgacgcacg
    11041 gtccgacaat cagtgcggac agaataccca taataaagac ggaaacgatt ttaccgccgg
    11101 agagcctgct gctctggttt tggaaatatg actgtacggc gttgggaagc tggatgttga
    11161 acagcccgaa catagacagc gccaagacga ccatcaaagc cgatgccgcc aacacgaccc
    11221 aagcctgctg caaccatacg gtcagcagcg cgcccgtcag tccggcaaca atgccgacca
    11281 gcgtataagt cagagccaaa ccctgaacat aaacgacgga cagcacaaac gcccgcgcct
    11341 tgcccgcctt tttgtcaccg accacgatac tggacacaat cggcagcagg ggatacatac
    11401 aagcggtaaa actcaggccc aaaccggcga gaaaaaacgc caaaagattg gcgttgagcg
    11461 tatcccaaga cagcttgaaa cggccgtcgc cgccctcgtc ccccttcggg ggcggcaacg
    11521 ccccgctgcc gttttgagag gaaggctgca aaaaacggtc tttggcggat gccggttcgt
    11581 cggtttgcgg atggtaagtg ccgttgccgg aaatatcaaa ctcggtatcc acgggcggat
    11641 agcacacgcc ggcttcggca cagccctgat aggtcaaaac caatttatac ggttcgccga
    11701 cagcctttgc ataaggaaag gcaacctgcg cctcgtgatg gtgaaccgtc tgcctgccga
    11761 aaaactcgtc ttccttctct tcgcccttac tgaaagaagg ctgtcccaac aaatccgccg
    11821 gatcggtctt gccgacgatt ttcgcctgat acatatagta tccgtcggca atcctgaaac
    11881 ggacgttcac gccgtcgtcg gcaacagtaa gctccggcac gaatgccttt tccggcggca
    11941 gcagatcgtt cgcatccagc gcgaaagccc gtccgcacaa catcaaaaat acggcgaaca
    12001 ggcaaatcag ttttttcata atcgaatccg tttcagacaa ataatttgtc cgcattataa
    12061 atggtaaggt tgacggtagg atttaattta tgtaaaaccc gccattatcc taacctattt
    12121 ccataaacat cttatcggac ccgccatgta cgatgtcaat acccacgatg tccgccgctt
    12181 tttcgcccgc gtgtggcagc agcggctcaa tccgctgcaa ttgggcgcgt tggaacagaa
    12241 agccctccgc attgtcgaag cccatcccga ataccaccgt tatctcgaac gcatcgaaga
    12301 ccatctggac accgactggc tgcccgaaaa cggcgaaagc aaccccttcc tgcatatgtc
    12361 gctgcatttg tccgtccaag aacaggcggg catagaccag ccgcacggca tacgcgccat
    12421 ccacgacacc ctgtgcgcca aacgcggctg gccggaagcc gaacacgaaa tgatggaggc
    12481 actggcggaa acactgtgga cggcgcaacg ctacggcacc ggtttggatg tcaatttcta
    12541 catgacccga ctgcgcaaac tcatcggctt gggtgcagag gatcaagcca ggttaaaccc
    12601 gcatgaaatc gcctgaccat accgaccgcc tgcaaaatgc cgtctgaagc ggaacaaccc
    12661 ctttcagacg gcattcattt tcccccaatc atttccacaa cgcgcgtttc aacataatca
    12721 accaatcctt cttatccaaa acggggcgtt gtgcaaacac atcgtatcgg cacgcgtcca
    12781 gtttctgcaa aatcaactgc gcccccaaca caatcatacg gagttccaaa ccgatacgcc
    12841 ctttcaattc gcgcgccaaa ggcgaacccg ccttcagcat acggaatgca cgccggcact
    12901 catacgccat cagccgctga aacgccgcat ccgcccgtcc tgctgcgatc tgttcctcag
    12961 aaacaccgaa tttcaacaaa tcgtcctgcg ggatataaac cctgcccttt tgccaatcca
    13021 cagctacatc ctgccaaaaa ttcaccagtt gcaaagccgt acaaataccg tcgctttgcg
    13081 ctacgcacac cgcatccgtt ttcccgtata aagccagcat aatgcgtccg acagggttgg
    13141 cggaacgccg gcaataatcg gtcagatcgc cgaaatgcgc gtaccgcgtt ttaaccacat
    13201 cctgcgaaaa cgccgagagc agatcataaa acggctgcaa atccaaaccg aacggcacaa
    13261 ccgcctcggc atccaatcgt gcaatcaaag gatgcgccga ccggccgccc gatgccaaca
    13321 cgtccaactc gcgccgcaaa ccctccaacc ccgacaacct ggcttcagac ggcatactgc
    13381 cctcgtccgc catatcgtcc gccgtccgtg caaacgcata caccgcatga accggcttcc
    13441 tcaacctgcg cggcaaaacc agcgaaccga cgggaaaatt ctcataatgc ccaaccgaca
    13501 taccttctcc atccatcaaa caaaatgccg tctgaaacgg aacaaaccct tttcagacgg
    13561 catcagatac ctccaagctg ccggcaatca gtggtggtaa tgaccgtgcg aaccgtggac
    13621 atgaccgtgt gcgatttcct catcggatgc atcgcgcacg ctttcaaccg tagctttaaa
    13681 gcggattttc atgcctgcca aaggatggtt gccgtccacc accgccttgc cgtcggcaac
    13741 atcggttaca cgatagacga caacatcgcc ggtttcagga tcgtcggctt caaacatcat
    13801 gccgacttcg acttcaacag ggaacgcgcc cgcatcttca atacggacca actccgaatc
    13861 ctgctcgccg aacgcatcgt cggacgaaag cgccacatcg accgtatcgc cggcatcctt
    13921 accgtgcaac gcctcttcca ccaaagggaa aatgccgtcg taaccgccgt gcagatacgc
    13981 aatcggttct tcggttttat ccaaaagctg attgttggca tcatacatct cataatgcag
    14041 cgaaaccacg gaattttttg caatagccat atttgtcctt tcaggaacag cagattgatt
    14101 acaggcgcat tctaacacaa ccgccgcgcc ggccgattac cgttaacctg ttcataaact
    14161 gtacggcaca tatttcaatg taaatttttg ttattttatt gcagtgtaaa ttttttacaa
    14221 cactctcaaa aaccattccg acccatctgc cgacttcccc aatctgcctt aataaatcat
    14281 acaagatact gaattatatt aatctctata atatttatcc ctatcggatt tttaacagca
    14341 aaaccgtttt acaggattta tcaatccgcc cgccagaaaa cttttcattc aaaccttttt
    14401 cccatctgta cgacattgca atcccttatt ccatggtgca taattacgca aattcag
//
LOCUS       DAWTHD010000062        14344 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWTHD010000062 DAWTHD010000000
VERSION     DAWTHD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.062"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(480..968)
                     /locus_tag="WM704_001377"
     CDS             complement(480..968)
                     /locus_tag="WM704_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340790.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPIIHQINQKKTRN
                     LF"
     gene            complement(1186..2106)
                     /gene="truB"
                     /locus_tag="WM704_001378"
     CDS             complement(1186..2106)
                     /gene="truB"
                     /locus_tag="WM704_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFA9340791.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDNLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            complement(2164..2688)
                     /locus_tag="WM704_001379"
     CDS             complement(2164..2688)
                     /locus_tag="WM704_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340792.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            complement(2699..3070)
                     /gene="rbfA"
                     /locus_tag="WM704_001380"
     CDS             complement(2699..3070)
                     /gene="rbfA"
                     /locus_tag="WM704_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFA9340793.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            3253..4497
                     /gene="clpX"
                     /locus_tag="WM704_001381"
     CDS             3253..4497
                     /gene="clpX"
                     /locus_tag="WM704_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFA9340794.1"
                     /translation="MSNENRTCSFCGKSKSHAKHLIEGKNAYICDECVANCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(4604..5797)
                     /locus_tag="WM704_001382"
     CDS             complement(4604..5797)
                     /locus_tag="WM704_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFA9340795.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(5896..6105)
                     /locus_tag="WM704_001383"
     CDS             complement(5896..6105)
                     /locus_tag="WM704_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340796.1"
                     /translation="MGWISSKRKKAPHVCVVPIAVSGCKRFALHDEMPSEDKNIGEIL
                     NQNAAAPQALYRNFFVFFSYPVENI"
     gene            6202..6702
                     /locus_tag="WM704_001384"
     CDS             6202..6702
                     /locus_tag="WM704_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340797.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            6787..7341
                     /locus_tag="WM704_001385"
     CDS             6787..7341
                     /locus_tag="WM704_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340798.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            7355..7555
                     /locus_tag="WM704_001386"
     CDS             7355..7555
                     /locus_tag="WM704_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340799.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            7686..7792
                     /locus_tag="WM704_001387"
                     /pseudo
     CDS             7686..7792
                     /locus_tag="WM704_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7906..9294)
                     /locus_tag="WM704_001388"
     CDS             complement(7906..9294)
                     /locus_tag="WM704_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9340800.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            9622..10569
                     /locus_tag="WM704_001389"
     CDS             9622..10569
                     /locus_tag="WM704_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9340801.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(10621..10953)
                     /gene="trxA"
                     /locus_tag="WM704_001390"
     CDS             complement(10621..10953)
                     /gene="trxA"
                     /locus_tag="WM704_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFA9340802.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            11251..11724
                     /locus_tag="WM704_001391"
     CDS             11251..11724
                     /locus_tag="WM704_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFA9340803.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            complement(11795..12616)
                     /gene="nadE"
                     /locus_tag="WM704_001392"
     CDS             complement(11795..12616)
                     /gene="nadE"
                     /locus_tag="WM704_001392"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFA9340804.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            12813..14168
                     /locus_tag="WM704_001393"
     CDS             12813..14168
                     /locus_tag="WM704_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFA9340805.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         3362 a         3752 c         3784 g         3446 t
ORIGIN      
        1 atgtatgtgc cattttgtta cctgatggct ctgacatttt tacagcaatg cagtttataa
       61 aattttctct tgtaaaagtt tcatctaata aaacttttaa ataagcctgt tccttatcat
      121 cgcattgaac aaagattagc ccatcatctt ttaataactc tttcgcaatt tccaagcgat
      181 ttttcataaa tgttagccaa gtggaatgtg aaaatttatc gttatatgca aagctatcgg
      241 tttctgtatt atacggtgga tcaatataaa tcaactttac tttgccttta aactgcttgg
      301 caagcgaatg cagggcaatc agattattgc ctttgataat aagattttcg gctggcgtac
      361 cgtctgaacg gcgtttgatt tcgccaacgg gttgtttgcc gtctgcggtg tggcgggaga
      421 attttgagaa tgcttttgcg tcaaaaagcc ggtcaatttc atcaaaagca agcgtttgat
      481 taaaaaagat ttcttgtctt tttctggtta atttggtgta taattggctg gctggctggc
      541 tggctggctg gctggctatt attgcgttta aaataaattt cttcgccttc ttcggtgctt
      601 tgtccgccat tcaatacgca atctttaaac ggaaaatcca acacaatatc cgaattgtct
      661 ttcaaaaagc ggttgccgtc cgtcaaacca atgcggttgg cgtattttgt gtaggaatta
      721 ttgatgctgt gtttgtccaa gaaaaaacgg aaatcctgca atttaaacac caaaacacca
      781 ttcacttcca caaagaaatg gcgttttaac tcatcattat tcaataacaa accgataatg
      841 gacgggtcgg ttttttccac caaatccaac aggatgtttt tggccaaaat ggttttttct
      901 tcgttcgccc atattttttc gtgtgatagc agggcttggg ctaattcggt ttgaatgcct
      961 gtcttcatcg ttataaatcc tcatcagtta ttttaatgtg ccattcttgt gtaacggcaa
     1021 atggctttac aaataaatta taataaaatc aattctttga tgtctttgct gtttccgggg
     1081 gaagttcgca aagttgcgat aagcggggcg ggtgtccgag tgtgtcgtga ttttacggaa
     1141 atatcaacac attattcaaa cgcagcccgt atttttaacc gccgctcagg cggaggatgc
     1201 cgccgtgttc atcaggcgca aggctttcat acggcatatt tccttttgat attccgccaa
     1261 accgacaaag cggccgtttt ccgtgtaaac gcgtaccggc gtatcggaag gcaggtcttc
     1321 ctcgaaacgc ggccgttgcc cgcattggag catatggacg gcataatcgt ttaaaacggt
     1381 ttggggaaag tgtgaaacca atacgtcgca gggcagcagc aggttgtcgc gttctgtttc
     1441 gtccaagttt gccaaggctt cgagcgtgtg gctttgggcg atggtaaagc cggcggtttc
     1501 ggtgcggcgt agggcggtca ggtgggcgaa tgtgccgatg tgtttggcga tgccttcgct
     1561 gagggtgcgg atgtaggtgc ctttgctgca acgcacgctt atcacggctt tgggcgcgtc
     1621 aaactcggca atatcgatag agtaaacggt aatgtcgcgc ggtttgcgtt cgataacaat
     1681 gcctttgcgc gcgtattcgt acaacggttt gccttcgtgc ttgagggcgg aaaacatcgg
     1741 cggcacttgg cggatgttgc ctgtcagtgc ttggcaggcc gtctgaaatt cggctaagga
     1801 aatatcggcg cgggcggcgg cgatgatttc tccttcggca tcgcccgtgc tgctggcttc
     1861 gccgagtttc agcgtggcgg tgtaggcttt atcggcatcc agcaggtatt gggcgaactt
     1921 ggccgcttcg ccgaagcaga cgggcaaaag tccggttgcc aaaggatcga gcacgccggt
     1981 atgccctgct ttttcggcat gaaacaaacg ccgcgctttt tgcagggcgg tgttgctgga
     2041 aaggccttcg ggtttgtcga gaagaagaac gccgttgacc gggcgtttgg cgggtttatt
     2101 ggtcatatta attagggaat attaatgttc agaccgtctg aaagggttcg gacggcctgc
     2161 gtgtcagctt aattcttcat tcaataattc acgaacagag tgtttttcat caattagatc
     2221 aatgctgatt gcatcaaagc catttcgttt taatgagtca aaactaatga tgcggccatc
     2281 aattaagggg acttgtcctt cagaattgac gaaatcttca tattcatctt cgtagtctat
     2341 attgtaataa agatgaatcg gaactcggta agtatattca tccaaatcag ttaacgttct
     2401 gttttgcttt tgattttggc tatatttgtc gcagaacata gcgttatagc catgttccgc
     2461 cccatcaaac aatacgattt catcgccgct tacagcttct accgccacaa tttttaccgg
     2521 cgccttatca gtatcggtaa tcaatccatc aaaaggctct ccatagtaac ggatgtggaa
     2581 agtatctcta ccttggcagg ataatgaaaa gcaacgatgc ccgccttcaa tttgcaggtt
     2641 atcactatgt gcataggcgg ctaaatgttc gggaatcaga ttgttcattc tggcagtatc
     2701 agtcttcaac cggtttttcc gccgccactt gatcgataag ggcggacagg ctcataccgc
     2761 gttccaaaga ttcgtcgtat ttgaaatgca gttcggggat tttgaacagc ttgatgcgtt
     2821 tggacaattc gctgcggagg tgtccgcgcg cgtgttccag cacttcttcc gtaatttcgc
     2881 gcgtatcttg gttcaaaacg gtgtagaaca cggtggcgtg gctgtaatcg cgggtaactt
     2941 cgacttcgtt gatagtgatg aagccggcgc gcgggtcttt cagtccggta cggacgagtt
     3001 cggcaagctc gcgcataatt tgttctttga cacggtcttg gcgggcgtag ccgcgttggg
     3061 gttttctcat gaatatcctc agatttggtg tcagacggca tttctgccgc cggggcggtt
     3121 gcgaaagggc gtattatagc cgaatcggcg ggattttaag gtaatacgtt gataagcacg
     3181 gatatagtta ttatacttgg caacccgaac ggcgcatgcc gtttctgcaa cgcatacatt
     3241 gtaaagaaag ccatgtccaa cgaaaaccgt acctgttcct tttgcggaaa atccaaatca
     3301 cacgccaaac acctgattga gggcaaaaac gcctatatct gcgacgaatg cgtcgcaaac
     3361 tgcctcgaaa tattgtacga aggcgacaac ggcggcacgc ctccggaaaa tgccggaggg
     3421 gagccggaag aatccggcaa actgcccacg cccgccgaaa tcgttgccaa cctcgacgat
     3481 tatgtcatcg ggcaggggca ggcgaaaaag gcgctggcgg ttgcggttta caaccattac
     3541 aaacgcctgc gccacccgaa agccgacggc ggtgtcgaat tgtcgaaatc caacatcctg
     3601 cttatcggcc cgaccggatc gggtaaaacg ctgttggcgc aatctttggc acgcaaactg
     3661 gacgtgccgt tcgtgatggc ggatgcgacc acgctgaccg aagccggcta tgtcggcgaa
     3721 gatgtcgaac aaatcattac caaactgttg ggcaaatgcg atttcgatgt cgaaaaagcc
     3781 cagcacggca ttgtctatat tgacgaaatt gacaaaattt cgcgtaaaag cgacaacccg
     3841 tccatcacgc gcgacgtgtc cggcgaaggc gtgcagcaag ccttgctgaa actgattgaa
     3901 ggtacggtgg caagcgttcc gccccaaggc ggacgcaagc atccgaatca ggaatttatc
     3961 aacgttgata ccgccaacat cctgtttatc tgcggcggcg cgtttgcagg cttggaaaaa
     4021 gtgattcgcc agcgcaccga gaaaggtggt atcggtttcg gcgcgtccgt tcacagcaag
     4081 gacgaaaatg ccggcattac caagctgttc ggcatcgtcg aaccggaaga tttaatcaaa
     4141 ttcggcctga ttccggaatt aatcggacgt ttgcccgtga ttgcaacttt agaagaactg
     4201 gatgaggacg cgctgattaa tattttaacc gagccgaaaa acgctttggt caagcagtat
     4261 caagccttgt tcggcataga aaacgtcggg ttggaatttg aagaaggcgc attgcgttcc
     4321 atcgcgcggc aggcaatgga acgcaaaacc ggcgcgcgcg gcctgcgttc catcgtcgaa
     4381 cgctgcctgc tcgatacgat gtaccgcctg cccgatttgc aaggcttgaa aaaagtggtg
     4441 gtcggcaagg cagtcatcga agagggcagg gaaccggaat tggtgttcga gtcctgatgg
     4501 caaacaaaac cgcttcggaa cgttggatca cgtttccgaa gcggtttttt gtttctgatg
     4561 ccgtctgagg ccttcccgtc ttcagacggc atctgctttc tgcctagggg cggtttgcgg
     4621 cggcaaattc cgtcagcacg tgttccaaac gccgcaaacc ttccgcaata tcctcatcgt
     4681 tcaacagcag cgaaggcgcg aaacgcaata cgtccgcacc cgcaactaag atcatcacgc
     4741 cgtgtttcaa ggaggtagcg gcgatttcgg atgagcgtcc gcgataaggc gtgtccaacg
     4801 cgcagccgag cagcagcccc atcccgcgaa cctgtgagaa caagcctgtt ttcctgccca
     4861 aatccagcaa tgccgtctga agtttctgcc cctgactgcg gacgttgtgc aaggtttccg
     4921 gcgcgttgat gatgtcgaag gcgcggctgc cgaccgcaca cgccatcggg ttgccgccga
     4981 aagtcgagcc gtgcgtgccc ggttggaagg cggcggcaat cttttctgtc gccagcatcg
     5041 cgccgatcgg aaagccgcag cccaaggctt ttgccgaact caaaatatcg ggaacaacgc
     5101 cgtaatgttc ataggcaaac agcctgcccg tatgccccat cccggtttga acttcgtcca
     5161 aaatcaacaa cgcattgtgc cggtcgcaca gacggcgcgc cgcctgcaaa tattccgcag
     5221 tggcgggcag gatgccgctt tcgccctgta tcggctcgat gatgaccgcg caggtctgtt
     5281 cgccgacggc agcttccagc gcggcaatat cgttgaacgg aacgtgcgta atgccttgcg
     5341 gcagcggtgc ataatcctta ctgtatttcg gctgtccgcc gacggacacg gtaaacagcg
     5401 tgcgtccgtg gaaactgttg atacaggcga cgatttcgct tttgcctccg ccgaaacggt
     5461 cgcgcgcata tttccttgcc agctttaacg ccgcctcatt cgcttccgcg cccgagttgc
     5521 agaaaaaaac cttgtcggca aaactgtgtt tcaccaattt ttgcgccaat tcctgcgccg
     5581 gccgcgtcgt atagatattg gaaatgtgcc acagcttgtg catctgcgcg tttaaagcat
     5641 cgacaagggc agggtggcag tgccccagag cgttaacggc gatgccgcct gaaaaatcaa
     5701 tatattcacg cccttccgta tcccaaacgc ggctgcctga agcgcgttcg ggaatcatcg
     5761 gggcaaaggc gaaattgggg gtcagatagt tttgcataat tgctcctgat tgcggttttg
     5821 ttcgggatta tgcaccgttt ttcaaggcgg ataaagggaa attgccgaca atatcgggca
     5881 gtggtaaaaa taaacctaaa tattttcaac aggatagctg aaaaaaacaa aaaaatttcg
     5941 atataatgct tgaggcgcgg cagcgttttg atttagaatc tccccaatat ttttatcttc
     6001 ggacggcatt tcatcatgca aggcaaaacg tttacaaccg cttactgcta ttggtaccac
     6061 acagacgtgc ggtgcttttt tgcgcttgct tgaaatccat cccatacgca aaacaggccg
     6121 ctcgaaaacg gggcggcttt tttgttacct tcacacaggt cgcccgattc ggcaacacac
     6181 gacagaatca ggaaaatgac catggatgca atgccccaaa ccgccgtaca aaacgaaaac
     6241 ctgcacactt gcagcattat tgtcgatgat gcgcaggatt tttcccccat gcttctcgat
     6301 gcagggcggg acgacacgct catcagcgaa tcgatgattc cgcaaatccg caccgttgcc
     6361 gccctgatag ccgccgaacg gcacgacttc agccgcagca gccccgccga gtttaccgat
     6421 gccgccgatt tttttgccgc acggattctc gtattgggcg tgcgacgctt ccatctggac
     6481 gtatcgctgc ttcagatttt gaaaaccgca aacaagcgcg cctgccgctt tgccgaaaaa
     6541 caccgcctgt ttttcacacc cgcacaagcc gaattgagcc tgaacaaaca caaaaaccgc
     6601 cgcctgctga ccatagaaac cgaacacgaa gtcgaaaaca aaggcaaccc tgtcgccaac
     6661 agcctcgcat ttgtccgcaa actgcacacc ctacccctgt aaacatcacg atttgccggc
     6721 agaatgcttg ccaaaattcc cgacggcgat acaatacaac cgattttcca cccgaacagg
     6781 acacacatga aaaaaatcat cgcctccgcg cttatcgcaa cattcgcact caccgcctgc
     6841 caagacgaca cgcaggcgcg gctcgaacgg cagcagaaac agattgaagc cctgcaacag
     6901 cagctcgcac agcaggcaga cgatacggtt taccaactga ctcccgaagc agtcaaagac
     6961 accattcctg cccaggcgca ggcaaacggc aacaacggtc agcccgttac cggcaaagac
     7021 gggcagcagt atatttacga ccaatcgaca ggaagctggc tgctgcaaag cctgattggc
     7081 gcggcggcag gcgcgtttat cggcaacgcg ctggcaaaca aattcacacg ggcgggcaac
     7141 caagacagcc ccgtcgcccg tcgcgcgcgt gctgcctacc atcagtccgc acgccccaat
     7201 gcgcgcacca gcagggattt gaacacgcgc agcctccgtg caaaacaaca ggcggcgcag
     7261 gcgcagcgtt accgcccgac aacgcgcccg cccgtcaatt accgccgtcc cgctatgcgc
     7321 ggtttcggca gaaggcggta aacccggcgc gccaatgccg tctgaagggc tttcagacgg
     7381 catttttgta tttgttaggg gcattgttat gttgccgttt gattttcaga cggcattttg
     7441 tttccaagcg tttgatgtcg ggatggcaat tctgatctac gtgcgttggc agggcggcat
     7501 tgtcataccg ccgttgggtt ttcagacggc atcatatcga accgtcaagc cgtagggcga
     7561 gattgggtag gcaggaatgc ccatcctgca tctgaaaccc ttctgcgtca ttcccacgca
     7621 ggaaggaatc tagatatttg aatttacgga aattttcaat cgttgccaaa gcggaaaagg
     7681 gtttttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat
     7741 agtacggcaa ggcgaggcaa cgcggtaccg gtttaaattt aatccactat attgcagatt
     7801 aatcctttgg gtatcaaagc cgggcggtgc tgaaacatta caaaaatgcc gtctgaaact
     7861 cgaaatcggt ttcagacggc attggtttac gcggcaggtt ggggcttagc ggtctttgta
     7921 acggcgttcg cttttgcctg caaaagagtc gccggctttg cgtttgcctt tgaagccttc
     7981 gccggttttt ttgaaactgt ctttcttaaa gccttctttc ttgaaacctt cgccgcgcgt
     8041 tttgccgccg aagccttctt tgcccggttt atgatcgccg cgccggccgc cggatttcct
     8101 atcgccccag ccgcctttgc ctttcggctt gccgcctgcg gatttgcgtt tgcgggccgg
     8161 ctccatgcct tcgatggtca gttcgggcag tttgcggtta atgtattttt cgattttgtg
     8221 gactttgacg tattcgttca cttcggcaaa cgtaatcgca atacccgtgc ggcctgcgcg
     8281 gccggtgcgc ccgatgcggt ggacgtagtc ttccgcctgt ttcggcaggt cgtagtttat
     8341 gacgtgggta atggtcggta cgtcaatacc gcgtgcggca acgtcggtgg caaccaaaat
     8401 tttgcagcgg cctttacgca aatccgtcag cgtgcggttg cgccagccct gcggcatatc
     8461 gccgtgcagg cagttggcgg cgaaaccttt ttcgtacaat tcatccgcga tgacttcggt
     8521 catcgctttg gtggacgtga aaatcacaca ttggtcgatg ttggcatcgc gcaggatgtg
     8581 gtcgagcagg cggtttttgt ggcgcatatc gtcgcagtac aacaactgct cttcgatttt
     8641 gccttggccg tccacgcgtt cgacttcgat aatttcagag tctttggtca gtttgcgcgc
     8701 cagtttgccg actgcgccgt cccaagtggc ggagaacaat aaagtctgac ggtcggccgg
     8761 ggtggcttcc acgatggttt cgatgtcgtc gataaagccc atatccaaca tacggtcggc
     8821 ttcgtccaaa atcagcactt ccaaacgttc aaaatcaact ttgccgcttt gcatcaggtc
     8881 cgtcagacgg cccggagtgg cgacaatcaa atcgaccggt ttgctcaggg cgcgggtttg
     8941 gtagccgaac gatgtgccgc cgacgatgct gacggtgcgg aaccagcgca tatttttggc
     9001 atacgccagc gcgtttttct cgacttgcgc cgccagttcg cgggtcggtg ccaacaccag
     9061 cgcgcgcggg cctttgcccg gtttttcgct gcgtttggtc agtcgctgca aagtcggtaa
     9121 cagaaaggct gcggtttttc cggagccggt ttgcgccgaa gccatgatgt cgcagccttc
     9181 caaagcaaac ggaatcgcct gcgcctgaat cggcgtcggg ctttcgtaac cctcgctgct
     9241 gacggcggac aaaatgtttt tatcaaggtt caaatcggca aatttaatag acatggctat
     9301 ccttggaaga caacaacgcc cgcgtccgaa aggttcagac ggcctgaagc agggaagggt
     9361 aacgatacgg ggaatgtgaa gttacagggt tgtcgcaagc ggcttgcttg tggtttacat
     9421 cgacggcaac ctgcacagat atgctttgca aaatcaaagc aacagaagaa acgcgcccgt
     9481 aagggcggtt taggttggaa ggcgatggct tcgtatgaca agataagcga cggatggtat
     9541 agggtttgcc cgaaaaggtc aagcaatcgg gcgttaatta cagtaaaatt tccgttttcc
     9601 actttattcc gcctgaaatc aatgaccgac atcacaccgt ttgccaaccg cttgggcaaa
     9661 aacatcaaac accttatgaa atgggcgaaa cgcaacggca tcgaagcctg gcgcatttac
     9721 gaccgcgaca tcccccaatt cccttttgcc gccgatgttt acggcgatcg aatccacctt
     9781 caggaatacg ataccggctg gctgatgcgg cccggagaat acgaagcgtg gcttgccgaa
     9841 gtattggaag ccgtcgcttt cgttaccggt tttgaacccg aacaaatccg tctcaaacgc
     9901 cgcgaacgcc aaaaaggttt gcagcagtac gagaaaaccg gcaaggcggg cgacgatttc
     9961 gtcattgccg aaaacggccg caagttttgg gtcaacctcg acaaatacct cgacaccggc
    10021 ttcttcctcg atcaccgcaa cacgcgcaaa aaagtcggcg aaacggcggc gggcaaacgt
    10081 tttttgaacc tgttttccta caccggcagc ttcaccgtct atgccgccac cggcggcgcg
    10141 gcatccagcg aaaccgtcga tttgtccaac acttatctcg attgggcgag gcgcaatttt
    10201 gaattgaacg gcatcgatat ggaacggcac aaaatcgtcc gcgccgacgt gttccaatac
    10261 cttcagacgg catatgggga aggcaggcag ttcgacctca ttgtgatgga cccgcccagt
    10321 ttttccaaca gcaagaaaat gcccggcatc ctcgacatcc agcgcgacca tcaaaaactc
    10381 atagacggcg cggtgaacct gctcgcttca gacggcattt tgtatttttc caacaacctg
    10441 cgcagcttcg ttttggacga ttcggtatcg gaacaatacg ccgtgaaaga catttccaaa
    10501 caatccgttc ccgaagattt ccgcaacaaa aaaatccacc ggtgttggga aatccgccac
    10561 aaatcttaag ggtgcgaaaa agccgccgga atcttccggc ggcttttcga ttgcgcttta
    10621 tcaggcgatg gaggcttcga caaaggcggt cagctgacct tttgccaatg cgccgacttt
    10681 ggtggcgacg acttcgccgt ttttgaacac catcagggtc ggaatgccgc gcacgccgaa
    10741 acgggacggg gtggcttcgt tgtcgtcgat gttgattttg actactttca gacggccttc
    10801 aaattcggcg gcaatgtcgt ccaaaatcgg ggcaatcatt ttgcaggggc cgcaccacgg
    10861 agcccaaaag tccagcagga cggggaggtc ggatttcaaa acatcttgct cgaaggcggc
    10921 atcgccggtg tgtacgatta attcgctgct cattattttt ccttttcggt tgggattcgc
    10981 acgatagcgt gcaggatagg gcttgggcat aaaaatttca agtgggcggg cgggataata
    11041 gtttttctgt accgccccaa tcgcctttgc aaattctaaa aaacggttcg gacagtattt
    11101 tatttttgaa aattactttt attatcaaag tataaggaat gtttttgagg ctgtacagaa
    11161 acgcgcgggc ggtgtatgct ccgtttaaat agattcttaa agtatgaatg ttcaaaacca
    11221 aacccgaaac cctataaaag gaacactgca atgactgatt tttccgtttg ggaagctgct
    11281 ccctttggcg caaccgttga tcatatcctg caacgctacc acaatgtcca ccgcgcacaa
    11341 tttgaagaat tggtgccgct ggcgcagaaa gtggcccaag tccatgccga tacctttccg
    11401 gcggaaattg ccgggctgct tgccgatatg cgggacgagc tgctgatgca tatgatgaag
    11461 gaagagcgga tgctgtttcc gatgattaat cagggtgtcg gacgcggtgc ggcgatgccc
    11521 atcagcgtga tgatgcacga acatgaagaa cacgaccgcg ccatcgcacg gctcaaagag
    11581 ctgaccggta atttccacgc acccgaaggg gcttgcggaa gttggacgcg cctttacgcg
    11641 ctggcaaaag aaatggcgga cgatttgaac gaccacattc atttggaaaa cgatattctg
    11701 tttgcccgcg tactcgattc ttaagggata ggagtcggat atttcaatgc cgtctgaaat
    11761 acggtttttc cgtttcagac ggcattttca tgtttcagcc cagcaattcg ggcggaatgc
    11821 ggcacacagg aatcgggttg attttgtgct gcatcgcgcg gtgaagccgc gtatagattt
    11881 ccagaacttc gcgccgccgt ccttcaaaat cttcgggttt gcgcgtgccg tacacgccca
    11941 ttgcccactc cagttcggga tagcttgcgc ccatctgttc ctcgtcggtg cgttccgtat
    12001 cccacaggcc gtcggtcggc ggggcttttt gaatcgcctc gtccacgccc aatgcttcgg
    12061 caagccggta aacctgcgtt ttggtcaggt cggcaatcgg gctgatgtcc acgccgccgt
    12121 cgccgtattt ggtaaaaaaa cccacgccga aatcttcaac cttattacct gtccccgtaa
    12181 ccagcagtcc gtgtatctgc ccgtagtagt acagggtcag catacgcagg cggcttctgg
    12241 cgttggcgag cgaaagcggc tgattggcaa atgccgtctg atgaacgccg acggtttgct
    12301 caaaggtttg gaaggtgtcg gtcagattgg ccgtttgcgc gcttacattg gcatattgcc
    12361 cttgcagatt gcggatgtgc cgccttgccc gctcgagctg gccggggtgt tggcgtatcg
    12421 gcatatccag aagcagcgtg gtgcggccgg tgcgggcggc gagcgtggag acgacggcgg
    12481 aatcgatgcc gccggaaacg cctacgacga acccttttgc atttgcctga gcggcgtatt
    12541 cgtccaacca tcggacaata tgtgtgataa ctgcctgcgt atccattttt cctcctgtcg
    12601 aaaatatccg catcatagcc tgccggcggt gcaatttgaa tagattgagg gcgggttgat
    12661 gaatattttt cccattcccc gcttaaaaaa gtcatatagg aaaaggctgg cggcaagatg
    12721 gcgcgtgtta taatattgtt gacaatttag acggcacgat gccgccttgg gcttcaaaac
    12781 atattcatat tttttattat ttgattgatt cgatgtctga tttttttcat tctgacgtgt
    12841 tgtccgtttc cgagttgaac gcatttgcca aaagtatttt ggaaaaccac cttgccggcc
    12901 tgtggattgc gggcgaagtg tccaatctga cccgcgccgc cagcgggcat tattatttct
    12961 cactcaaaga cagccgcgcg caggtgcgtt gcgcgatgtt caagggtgcg gcggcgcgtt
    13021 tggcgcaacc tttgaaagaa ggcgaccata tcgaagtggc gggcaaaatc agtatttacg
    13081 aggcgcgggg cgaatttcag attaccgtaa acgaagtacg gctcaaaggt ttggggcagc
    13141 tttacgaagc ctacgagcgg ttgaaggcgc agttgcaggc ggaaggcgcg tttgcggcgg
    13201 aacgcaaaaa acctttgccc gtccgtccgc aatgtatcgg catcgttacc agtctggcgg
    13261 cggcggcttt gcgcgatgtc gtaaccacct tgaaacgccg cgcgcccgaa atccccgtca
    13321 tcgtttatcc ggccgccgtt cagggcgcgg gcagcgggtt tcagattgcc caagcgatta
    13381 aaaccgcatc gcaacgcgcc gaatgcgatg tgctgattgt ctgtcgcggc ggcggcagca
    13441 tcgaagactt gcgggcgttt aacgaagaac cggtcgtgcg cgccatcgaa gcctgcacga
    13501 ttcccgtcgt cagcggcgtg ggacacgaaa ccgatttcac gctcgccgat ttcgttgccg
    13561 acgtgcgcgc gcccacgccg accggcgcgg cggaactggt cagccccaac cgccaagaat
    13621 cactgcaccg cctcgtccaa gcccaaggcc gtctgaaaac cgttttggag caacgctatt
    13681 tcgatgccag ccaaaaactc gactggctcg cgcggcaaat ccgccacccg cgccaaaaac
    13741 ttgacgagca gcgcgcttca atcggcaagc tggcgcaaac gctgtcttac tcgatgacgc
    13801 aaaacctccg cgcccacacc gcccgttttg aacgccaaac ccaagccctg caacattgcc
    13861 gtcctgatgt ttccgtttac cggcaagata tcgtccgcct tcagacggca ttgcccgccg
    13921 ccttcagccg gctgctcgcc cgtcgccgtc aaagcctgac cgcccaagcc gcattgctcg
    13981 aagccgtttc gccgcagcac attctggaac gcggcttctc cgttgtcaaa aatacacgcg
    14041 gacaagtcat ccgtaacgcc gatgttttga agcaggggca gaagctgcac attaccttct
    14101 ccgacggcga aaccgacgtg cgcgtaagca aagaacaagg gcagcaggat ttgttcgatt
    14161 gcatttaaac ccaatcggaa aaaccgctcc ccctccgccg tcattcccgc gaaagcggga
    14221 acccagtccc ttgaccttaa gataatcttt aattcgtcat tcctgcaaaa gcgggaatcc
    14281 ggaagcggaa agccgcagga atttgtcgga aaaaacagaa acctctccgc acacccgaaa
    14341 tgtt
//
LOCUS       DAWTHD010000063        14320 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWTHD010000063 DAWTHD010000000
VERSION     DAWTHD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14320
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.063"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(154..2430)
                     /gene="metE"
                     /locus_tag="WM704_001394"
     CDS             complement(154..2430)
                     /gene="metE"
                     /locus_tag="WM704_001394"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFA9340806.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(2566..3444)
                     /gene="metF"
                     /locus_tag="WM704_001395"
     CDS             complement(2566..3444)
                     /gene="metF"
                     /locus_tag="WM704_001395"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFA9340807.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            3641..3916
                     /locus_tag="WM704_001396"
     CDS             3641..3916
                     /locus_tag="WM704_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFA9340808.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            3916..4041
                     /gene="ykgO"
                     /locus_tag="WM704_001397"
     CDS             3916..4041
                     /gene="ykgO"
                     /locus_tag="WM704_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFA9340809.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(4127..4345)
                     /locus_tag="WM704_001398"
     CDS             complement(4127..4345)
                     /locus_tag="WM704_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340810.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            4488..5627
                     /locus_tag="WM704_001399"
     CDS             4488..5627
                     /locus_tag="WM704_001399"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFA9340811.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            5624..6205
                     /gene="metW"
                     /locus_tag="WM704_001400"
     CDS             5624..6205
                     /gene="metW"
                     /locus_tag="WM704_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFA9340812.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            6322..6513
                     /locus_tag="WM704_001401"
     CDS             6322..6513
                     /locus_tag="WM704_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340813.1"
                     /translation="MKNKTSSLPLWLAAIMLAARSPSKEDKTKENGASAASSSASQSD
                     LQPAASAPDNVKQAESAPL"
     gene            6556..7119
                     /locus_tag="WM704_001402"
                     /pseudo
     CDS             6556..7119
                     /locus_tag="WM704_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
     gene            7117..7224
                     /locus_tag="WM704_001403"
                     /pseudo
     CDS             7117..7224
                     /locus_tag="WM704_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7300..7860)
                     /gene="efp"
                     /locus_tag="WM704_001404"
     CDS             complement(7300..7860)
                     /gene="efp"
                     /locus_tag="WM704_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFA9340814.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(7903..9054)
                     /gene="earP"
                     /locus_tag="WM704_001405"
     CDS             complement(7903..9054)
                     /gene="earP"
                     /locus_tag="WM704_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFA9340815.1"
                     /translation="MNTYAFPACWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(9073..9351)
                     /locus_tag="WM704_001406"
     CDS             complement(9073..9351)
                     /locus_tag="WM704_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340816.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            9702..10643
                     /gene="miaA"
                     /locus_tag="WM704_001407"
     CDS             9702..10643
                     /gene="miaA"
                     /locus_tag="WM704_001407"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFA9340817.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            10647..11366
                     /locus_tag="WM704_001408"
     CDS             10647..11366
                     /locus_tag="WM704_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFA9340818.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            11371..11859
                     /locus_tag="WM704_001409"
     CDS             11371..11859
                     /locus_tag="WM704_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFA9340819.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILLILLVKYIAY
                     LF"
     gene            11925..12677
                     /gene="rlmB"
                     /locus_tag="WM704_001410"
     CDS             11925..12677
                     /gene="rlmB"
                     /locus_tag="WM704_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFA9340820.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEGILENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDIGGNADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(12739..14113)
                     /locus_tag="WM704_001411"
                     /pseudo
     CDS             complement(12739..14113)
                     /locus_tag="WM704_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
BASE COUNT         3511 a         3934 c         3700 g         3175 t
ORIGIN      
        1 ctccgatttg cctgttcccc acatataaag ccgaattaga gaaaaacaag ctgcaatgaa
       61 aagattttcc ggcaactgct cccctgcgcc gcaactcagg acaaaatgcc gtctgaaagg
      121 ctttcagacg gcatttgttc atacggtctc ggcttatttc gccaattcgg cacgcagttt
      181 gcgggttacg ttcatcatta cttgaagctg ttccagagtt tctttccagc cgcgtgtttt
      241 caggccgcag tccgggttaa cccacagacg ctcaaccgga acaacctcaa tggctttgcg
      301 caacaggtgt tccacttcgg cttctgtcgg tacgcgcggg ctgtggatgt cgtaaacgcc
      361 cgggccgatg tcgttcgggt atttgaattc gccgaacgca gccaagagtt ccatgtcgga
      421 acgtgaagtc tcaatggtga tcacgtccgc atccattgca gcaatcgcag gcaggatgtc
      481 gttgaactca gagtagcaca tatgagtgtg gatttgagtg ctgtcttcgc aacctgcaga
      541 ggataggcgg aaggattcgc ccgcccagtt caggtaggca tcccaatcgg cacgtttcaa
      601 aggaagacct tcgcggatgg caggttcgtc aatttggatg actttaatgc cggctttttc
      661 cagatccaat acttcgtcgt tcagagccag tgcgatttgt ttgcacacgg tagcgcgcgg
      721 aatatcgttg cggacgaaag accattgcag aatggttaca ggaccggtca acataccttt
      781 catcgggcgt ttggtcaggt tttgtgcgta agtagaccaa gcgacagtca tagcttcagg
      841 acggcttacg tcaccgaaga tgataggtgg tttaacgcag cgtgagccgt agctttgcac
      901 ccagccgtat tgggtgaatg caaaaccgct caacagttcg ccgaagtatt cgaccatgtc
      961 gttacgctcg gcttcgccgt gcaccagtac gtccaagtcc agtttttctt gctcttcaac
     1021 caccaaggcg atttcttttt tcattgcggc ttcgtaatcg gaggcagaca gttcgccttt
     1081 tttgaaggct gcgcgtgctt ggcggatttc ggtagtttgc gggaaagaac caatgttggt
     1141 cgtcggcagc agaggcaggt tcaaccatgc ttgttgcgct ttgatacggt cggcaaacgg
     1201 tgatttgcgt tggtctgcgt tgacaggcag gtcggccaag cgtttggcaa cgtctgcacg
     1261 gtggatttca ctgctgttgg cacgggagtc ggcagcagct tggctggcgg ccagttcttc
     1321 ggcaacagaa tcacggcctt cattcaatgc ggctttcaga acacgcaatt cttgggtttt
     1381 ttgcagagtg aatgccaacc aagagtacag gtcgggtttg ttggctttca gtttttcttc
     1441 gactgacaag tcaaacggag tgtgcagcag cgagcaagag ctggaaatcc acaaacgctc
     1501 gcccagtttg gcttgcagag gcccgacggt ttccaaaact ttgttcaggt tggcgcgcca
     1561 aatgttgcgg ccgtcaatca cgccggcaga caggactttg tcgtagccgg cgaacgcgtc
     1621 cagttgctcg ggtgcgcgta ccaagtcgat gtgcaggccg tcaacaggca gggatttcaa
     1681 caatgcggcg tgttcggcaa cagaaccgaa gtaagtgccc aacaggattt tggcgcttac
     1741 tttgttcaaa gtagcgtaaa cgtctttgta tgcttctacc cattctttag gcagatcgac
     1801 agccaaagca ggctcgtcga tttgaatcca ctcggcaccg gcttcaacca aagcagtcag
     1861 gatttcaacg taaacaggca acagtttagg caacaggctc agacggtcga attcaacgga
     1921 gcctttttct ttacccaccc acaggaaagt caaaggaccg acaacggtcg gtttggcttt
     1981 caagcccaaa gtttgggctt cttgcagttg ttgaacgtag tgtttggcgt tggctttgaa
     2041 ttcggtatcg gcatggaatt caggcaccag gtagtggtag ttggtgtcga accatttggt
     2101 catttcgatg gcgaattggt ctttgttgcc gcgcgccagt tggaagaatt gttccaaagg
     2161 caggttttgg ctatcgaagc cgaaacgggc gggaatcgcg ccggtggcaa cttgcaggtc
     2221 gaggatgtga tcgtagaaag tgaaatcacc tacagcaacg taatcagcgt tggcagcagc
     2281 ttggtgtttc cagtttttct cgcgcaggtc tttggcaacg tcgagcagct cttgttcact
     2341 gatctctttg cgccagtatt tttcttgggc gaatttcaat tcacggaagg cgccgacacg
     2401 cgggaagcct gagaaatgta atgttgtcat gttgaactct ccagttggat tttgtaagga
     2461 ccggttcaat ccgaatcggc cggtattgta aatcagaatg gctcggatag ggcggcgttt
     2521 tgcttttcag acggcctcgg atgctgtctg aagccgtttt cggctttaag ggcgcgcgcc
     2581 taaaatatgg cagatggcgt aagtcagctc gctgcggttg agcgtgtaga agtggaaatc
     2641 tttcacgcct tcgcgggaca ggactttaac catatcgatg gcgatactgg cggcgacgag
     2701 gttgcgcgtg ccttggtcgt cgtccaagcc ttcatacatt tgcgacagcc atttcgggat
     2761 tttgacgttg gttacttgtg ccattttgcc gagttgcttg aagttggtaa caggcaggat
     2821 gccgggaacg atttcgacgt cgatgcccaa catcacgcag cggtcgcgga agcgcaggta
     2881 gcgttccaca tcgaagaaga attgggtgat gacgtggttc gcgcccgcat cgattttgcg
     2941 cttcagatta atcaggtcgg cttgtgcgga tttcgcttcg ggatgtactt cggggtatgc
     3001 tgctacagag atgtcgaagt cggcgacgga gcgtaatagc ttaaccaagt cttcggcgta
     3061 aaacggtttt ttctcataac cggccggctc gtcgccacgc agggcaacga tgcggcggat
     3121 accgctgtcc caatagtctt tggcgatttg gcgcaattcg tcgtgggatg catcgatgcc
     3181 ggtcaggtgc ggagctgctt ccaagccggt ttcctgtttg atgcgtttga cgatgccgtg
     3241 cgtgcggtcg cgttcgccgg agtttgcgcc gtaagttacg gatacgaact tgggatgcag
     3301 ggtttgcaga cggtggatgg aatcccacag catcgtttcc atttgttcgt ttttcggcgg
     3361 gaaaaattca aacgaaacgt tgatgttgcc tttcaaatcg gaaaggctgt tgtttaacgc
     3421 attgatttct tttgcataat ttatctgcat accttcgtta tcttttacat gatgtttcag
     3481 gcggtatgat aaggttgcac cgaatatgag tcaatttcaa aattttcagg tttatgatga
     3541 aaatttttaa tatttagcaa aaactatacg cttttcttgt acaagccttc taaattatga
     3601 taaagtttgt tatgttataa cacttcaatc aaggcaatat atgaaaccga atattcatcc
     3661 tgacaattat cgaaccgtcc tttttttcga cagcagtgcc aatgaaggct ggctgattcg
     3721 ttcttgcgcc ggaacgcacg gaaaaacaat ggtttggaca gacggcaaag aatatccgct
     3781 cttttcctta gatacttcct cttcctcaca tcccgtctat accggcaaac agcgtaacgt
     3841 caataccgaa ggccgtgcca gcaagttcaa ccaacgtttc caatccgtca tgtcatcgtt
     3901 tagaaaggac aaataatgca ggttctgtct tcgctcaaaa ccgccaaaca acgccaccgc
     3961 gactgccaaa tcgtcagacg caggggcaaa gtgtacgtca tctgcaaaag caatccgcgt
     4021 tttaaatcgc gccaacgctg accccctgcc cttcctgctt cggttgtaaa gcccctccta
     4081 ccgcaagccc ttttcaaata tgccgtctga aacagaaaaa tgcgtttcag acggcatttt
     4141 tccgtcgata cgaatccgga atgtgtttga aatacagcat aaacagatac caaaacagcg
     4201 tcgcggcggc tgcaccgagc agtacccatg cgctgatttc ggcgacttcg cgcaggacac
     4261 gtttctgcct gcggctaaag aggcggttga taatcagcgc ggcaagtgcg aaaaagaaaa
     4321 aaatacggaa aagtctgcct atcatgtcgg gaagcgaaaa gtatggttcg ggcgcggcgg
     4381 catgggaaag gatgtttcag cgcgtcaggc tgcttgtccg accgtgtcaa aaagtcatgc
     4441 cgtttgcgat ataataacgc ttttcgccgc atttgttgga acaggatatg agtcaaaata
     4501 cctcggtggg cattgtaacg ccccaaaaaa ttccgtttga aatgccgctg gttttggaaa
     4561 acggtaaaac tttgccgcgt ttcgatctga tgattgaaac ctacggcgag ctgaatgctg
     4621 aaaaaaacaa tgcggtttta atctgccacg cgctgtcggg caaccatcac gttgcgggca
     4681 ggcattcggc ggaggataaa tatacgggct ggtgggacaa tatggtcggt cccggaaaac
     4741 cgattgatac ggaacgtttt ttcgtggtcg ggttgaacaa tctgggcggc tgcgacggca
     4801 gcagcgggcc tttgtcgatc aatcctgaaa cgggcaggga atacggcgcg gattttccga
     4861 tggttacggt gaaggactgg gtaaaatcac aagccgcgct tgccgattat ctcggcatcg
     4921 aacaatgggc ggcggttgtc ggcggcagct tgggcggcat gcaggctttg cagtgggcga
     4981 tttcctatcc cgaacgtgtg cgccacgcct tggtgattgc gtctgcgccg aaactgtcca
     5041 cgcaaaatat cgcgtttaat gatgtagcac gtcaggcgat tttgaccgac cccgatttca
     5101 atgaaggaca ttaccgcagc cacaacaccg ttcccgcgcg cggtttgcgg attgcccgta
     5161 tgatgggaca cattacgtat cttgccgaag acggtttggg caaaaaattc ggacgcgatt
     5221 tgcgttccaa cggctatcaa tacggctata gcgttgaatt tgaagtagaa tcctatctcc
     5281 gctatcaagg cgacaaattc gtcgggcggt ttgatgctaa tacatatttg ctgatgacca
     5341 aagctttgga ctatttcgat ccggcggcgg atttcggcaa cagcctgacc cgcgccgtgc
     5401 aggatgtgca ggcaaaattc tttgtcgcca gcttcagcac cgactggcgt ttcgcgcccg
     5461 aacgttcgca cgaactggtc aaggcactga ttgccgccca aaaatccgtg cagtatatcg
     5521 aagtcaagtc cgcacacggg cacgatgcct ttttaatgga agacgaagcc tatatgcgcg
     5581 ccgtaacggc ttatatgaac aatgttgaca aggattgccg attatgaacc tgcgcgatga
     5641 tttgcaactg atttacgacc ggatacccga aggcagccgc gtcttagact tgggctgcgg
     5701 cgacggcgaa ttgctggcgg ctttggcgga acacaaaaaa tgcagcggct acggcatcga
     5761 aatcgacacc gacaacgtga ttgctgcgat gtcgcgcggc gtaaatgtta tccaagccga
     5821 tttggaagaa gggttgaccg cattcaacga tcaaagtttt gatgtgattg ttttgagcca
     5881 aaccatccaa gcgatgcaga ataccgaaaa aatcctgcgc tgcctgatgc gcctcgccaa
     5941 acaggcaatc gtcagtttcc cgaatttcgg ctactggcgc aaccgcgtcc aaatcgcact
     6001 cggcggacat atgccggttt ccgaacgtat gccctaccat tggtacgaca cgcccaatat
     6061 ccattggtgc acgctcaaag actttgattt gttgtgcgcc aaaaacaata tccgcgtcct
     6121 cgaacgcgcc gtgatgacgg gcaacagaca ggtcaaacat ttccccaacc ttttgggcag
     6181 ccttgcgttt taccgcgtcg ggtaagacag gttgaaaacc ttgtcggcaa taacgggttt
     6241 ctcggttcta ttaacaacag tccgtctgaa aaatgaaacg gcaggttttc agacggcctt
     6301 ttcaatattg gaaacaatag aatgaaaaac aaaacgtcat cacttccctt atggcttgcc
     6361 gcaatcatgc tggccgcgcg ttccccgagc aaagaagata aaacgaaaga aaacggcgca
     6421 tccgccgctt cgtcttccgc gtcccaatcc gatttgcaac cggccgcatc cgcccctgat
     6481 aacgtcaagc aggcagaaag cgcgccactg tgaaattgca ccggcctgca ccccgccgcc
     6541 ggcattggcg atctcataca gcaaatcgcc gaacacatcg actcggactg tctgtttgcc
     6601 ctttcccata acgaactgga aacccgtttc ggcttacccg gcggcggcta tgacaacata
     6661 cagcggctgc tgtttcccga catccgccct gaagatcccg actaccatca gaaaatcata
     6721 caggcaattg aagacttgcg ttacggaacg cgcaccatca gccggcaggc acaagatgcc
     6781 atgatggaac aggaacgccg cctccgagaa gcgacgctgc tgctgacaca aggcagtcaa
     6841 gaaacccgcg gacaaggcga ggaaccgaaa cgcgcacgtt attttgaagt ttcggcaaca
     6901 tctgcctatt tgaaccggca caacaacgga ctcggcggca atttccaata catcggccaa
     6961 ttgcccggct atctgaaaat gcacggagaa atgcttgaaa accaatcact cttccggctg
     7021 tccaaccgtg aacgcaatcc cgacaaaccg tttttagaca tccattttga cgaaaatggc
     7081 aaaatcacgc gtattgtcgt ttacgaaaaa aatatctata gtggattaaa tttaaatcag
     7141 gacaaggcga cgaagccgaa gacagtacag atagtacggc aaggcgaggc aacgctgtac
     7201 tggtttaaat ttaatccact atacttcaat ccaaacacgg ggcgaatata aaaaaatgcc
     7261 gtctgaaagc ctttcagacg gcatttttca agtaggatat caggcgcgtt tgcggaattc
     7321 gccggtacgg gtatcgattt cgactttgtc gccgttttcg atgtaagaca tcacttggat
     7381 ttcggtgccg ccgaccagac gcgcggtttt catcactttg ccggaagtat cgcctttgac
     7441 ggcaggctcg gtgtagtcga cttcgcgcac gatgatggtg ggcagttcga cagaaatggg
     7501 attgccttca tagaaggtaa cttcgcattg gtcttccata ccgtcaacga tgaatttcaa
     7561 cgcgtcgccg atgttgtcgg cttcgatttc gtattggttg aattcttcgt ccataaagac
     7621 gtacatcggg tcggcaaaat agctgtacgt acagtttttg cgggacagga tgaccacgtc
     7681 gaatttgtcg tcggctttgt aaatggtttc ggaagcagcg ccggtcagca ggtttttcag
     7741 tttcatgctg actttggcgg aagagcggcc gcctttgatg tattcggttt tttgaacgac
     7801 cataggatcg ttgccgacca taaatacatt gccggcgcgc agttcttgtg ctgttttcat
     7861 tgtgattttc caatcaaatc ggttgtcgta aaacgcgcta ttctagcgta ttttttgatg
     7921 ctttgaaaca aaggcggcga gtttttcgga tgcggaaggc tgcccgaaaa gataacggct
     7981 ccaatcctcc gcgccttgcc gccagccgtt ttgatgttgt tgcagggttt gccaacattc
     8041 gaggcgttgc gtggcggata aagcctctcc gccgttgagg tcgtccgaaa ggaggcggtg
     8101 caccgatgcg gtttcgggcg tgtagaagcc gtatgcctta tcccaaaagg cgtggagttt
     8161 gtcgagatgg acgttttcat cttgcgggta gatgtgccaa aagaaaggtt ttccggcaag
     8221 ctgggtacgc acgaaactgt cttcgccgcg tatcacggcg cagtcggcga ggtgcagcaa
     8281 tttgtcgaag tcctgttgcg gcacgaacgg gattttgaca aggcggacgg atgccgtctg
     8341 aaagacaccg ccgtcatttt gcagggcgtt ttgcggaata acgccgcttt gtttgaggct
     8401 gtcgataatt tgcgcccccg ccagcagtag ggtcatcagg ctgcctgcct gttgccacat
     8461 gtccagccac tttgcccaaa catcgccccg atagccgaaa agcagccatt cgggggcgtt
     8521 tttttcgggc agcaccagcc gccggcgcag ggcttcggta tcgaaacgga cggcttcgcg
     8581 gtaatcgcgt tcgcgtatca acccgccgct tttttcgctg aaacccataa accaaaaata
     8641 tttttgaacg ccctcctgcg gcgaaggcat caggtgcagc ctttcattgc tttcctccgc
     8701 gctcaaatat tcccaattca gccaaagcgg tttgtgtcgg cggatgatgt tcagcacatt
     8761 ttcgggcagg tcgcaggcaa aagtttcgat aacggcatcg ggaacgggcg cggtatcaat
     8821 gtctgccgca tcggaatgcc aagtgcggac atgaatatcc tgatgaacga agggaacatc
     8881 gggcaaatcg ggacaaagcg cgcgcaaggc ggacacgtcg tccgtccaca aatgcacctg
     8941 ccaaccgagt tcgcggtgca aaacacgggc gagccgccac gaaacgccga tgtcgccgaa
     9001 attgtcgatg accttgcaaa aaatccaaca ggcaggaaaa gcgtatgtat tcataaccat
     9061 cgggaaaaca agctaaaacg cgccgaaata gtggaacaaa tacacaacca gcaaaacaat
     9121 cggaataaac agaaacatgg tttgacgttt caaatcaaac ccttgtttaa gatgatgctt
     9181 gatggtttca aagaggacga atttaaacaa aaacgtcgct tcgcaggcaa ggatgatgcc
     9241 gttgatcaga tgctggggtt tgtcttcggg cggattggcg gcgaaaacgg caagcgtcgc
     9301 caaggggatg aggcagacgg cgagccagcg gacaagcagg tttcgggaca ttgcaaggct
     9361 ttcaaaaaag caaaacgcca ttatcgcctc aagaacgcac cttgtctaac ggacgcgccg
     9421 gagatgccgt ctgaagcctt gccgccggaa aacgccgcac cgccgaacct ttaaatatac
     9481 ccgggacggg cgttccccga gaaatcggac gctgccgccg atgcctgcgg acaaggcggc
     9541 atcgggcgcg ggcatattga aagcccgacg catcggtttg aacgcatttt ataaaggcgt
     9601 acggcaaacc cgccccttgc cgtcggcgtg cttccgcaaa gcaaaatata agggcggcgc
     9661 gctttccgct ataatccgca tttttccaaa cctgaaacat tatgccgacc ccgaaagcct
     9721 ttaccctgct cgggccgacc gcctgcggca aaaccgcgct cgccctcaaa attgccgaaa
     9781 cccttccggt cgaaatcatc agcctcgatt ctgcactgct ctataccggt atggacatcg
     9841 gcacggcgaa accgtccgcc tccgaacgcg cctttgtccc gcaccacctt atcgacatca
     9901 ttacgcccgt tcagacatac agtgccgccc gttttgtcga agactgcacg cgtctgaccg
     9961 gcgaaatcac ggcgcgtggg aaatgtccgc tgattgtcgg cggcacaatg atgtatttcc
    10021 gcgccctgac ccaaggtttg aacgatttgc ccgaagccga tgcctgcctg cgtgccgact
    10081 tggacgaaca aaaacagatg tacggtttgg attttctcta ccgcaccctg caaaaagtcg
    10141 accctgaaac agcatgccgt ctgaaaccga acgacagcca gcgcatcgga cgcgcccttg
    10201 aagtctatta cctgaccggc aggccgatga gcgcgcacct gaacggacag cccgaacaca
    10261 ccctgccctt cgaactctac accgccgccc tgattcccga aaaccgcgcc cgactgcacg
    10321 aaaacatcgc cctgcgtttc cacctgatgc ttgaacaggg ctttatcggc gaagtagaaa
    10381 acctgcgccg ccgctatccc ggcctgaccg ccgactcccc cgccatccgc tgcgtcggct
    10441 accgtcaggc gtgggaacac ctcgacggtg caaccgacag acaaaccttc atcgaaaaag
    10501 gcattgccgc cacgcgccaa cttgccaaac gccaactgac ttggttacgc aaaacacctt
    10561 tagactgcgt tgccgacccg ttttcagacg gcacttcagg cacgcgcctg attgaggcgg
    10621 caaaacggtt tttcggcgaa taaaccatgc tggccacgcc gccgctcgcc cccaaaaccg
    10681 tcgccgccct gcacaggctc ggtatccgta cgctcgaaga actgcggcaa aacggttcgg
    10741 tcaaagcctt tctgctgctc aaagcatcgg gtttgaccct cacgaaaagc acgctgtggc
    10801 aactcgagtc gctgctgaac ggcacgccgc cgcaagaaat gtcccaagcg cataaagact
    10861 gcctgcttgc cgagttgaaa aaccatccgc ccgtcgccgc ctttccgccg caggaagaaa
    10921 tgggatactt catgcgcgaa gcactgcgcc aagcagaaca atccgccgcc gacggcgaaa
    10981 tacccgtcgg ggcagtcatc gtttcagacg gcaaaatcat cacatcggca cacaacacct
    11041 gcattgccga ctgcaatgtc agccgacacg ccgaaatcaa cgccttggca caggcaggca
    11101 gcgaaatgca aaactaccgc cttgacggat gcgacatcta catcaccctc gaaccctgcg
    11161 ccatgtgcgc gtccgcactg atacaggcac gaatcaggcg cgtgatctac ggtgcggcag
    11221 aacccaaaac cggcgcggca ggcagcatcg tcaacctgtt tgccgacaaa cgcctcaata
    11281 cgcacaccgc catacggggc ggaatcctgc aagaagaatg ccgcgccgta ttaagccgtt
    11341 ttttccaaaa caaaagaaaa ggttgaaccc atgaccgccc tactcgtcat cctcgccctc
    11401 gccctgatag ccgtcggcac ggcaggcatc gtctatcccg ccctgcccgg cttggcattg
    11461 atgtttgccg gaacatggct gcttgcctat gccggcggct atcaaatcta cggcgcaggc
    11521 atcttgtgga cggtcggact catcagcctt ggcggcatac tggcggacta tatggcaggc
    11581 atgttggggg taaaatacac tggggcaggc aaactcgccg tccgaggtgc attggccggc
    11641 agcatcatcg gcatattttt ctccctgccc gggctgatac tcggcccctt tatcggcgcg
    11701 gcggcaggcg aactgatcga tcggcgcaat atgcttcagg caggtaaagc gggcttgggt
    11761 acgctgttgg ggcttgtcgt cggcacggcg ttcaaaatcg gctgcgccgt atccatcttg
    11821 cttatcctgt tggtgaaata catcgcctac ctgttttaac cccttcagac ggcataccgg
    11881 caaattgctg taatgccgtc tgaaatttcc gacaaagcac cattatggca aaccaaagac
    11941 ccatctacgg cttccacgcc gtcaacgccc gattgtggca aaaccccaaa tcgattaccg
    12001 aactctatat ccaagaaggc aaatccgacg cacgcacgcg cgaagtgctg gaaaaggcgg
    12061 caaacgaaaa catccgcgta tattttgccg atgccgaccg cctcaacgcc atcagcaaag
    12121 gcgcgcgcca tcagggcgtg gtcggattta tcgatgcctc caaaaaccac gtccacctcg
    12181 aaggcatatt ggaaaacttg agcgaaccgc cgctgctgct gatactcgac ggcatcaccg
    12241 atccgcacaa cctcggcgcg tgcctgcgta ccgccgacgc aatgggcgta cacgccgtca
    12301 tcgcgccgaa agacaaaagc gcgggactga acgccaccgt cagcaaagtc gcctgcggcg
    12361 cggcggaaac cgtcccctac atcaccgtaa ccaacctcgc ccgcaccctg cgcgaattga
    12421 aagaatacgg catttggatt atcggcaccg acataggcgg caacgccgac ctttaccatt
    12481 gcaatctgcc cgacagcgcg gcatgggtga tgggcaacga aggcgacggt atgcgccgcc
    12541 tcacgcgcga acattgcgac atgctggtgt ccatacccat gttcggcacg gtcgaaagca
    12601 tgaacgtctc cgtgtccgca ggaatggtat taagcgaaac ccgccggcaa cgcgtattga
    12661 aaaacaaaaa agcgtaagcc tcaaaaacaa tgccgtctga aacagggatt ccgtttcaga
    12721 cggcattcat tttcaaaatc agtgttccaa atggtcggta tcaaacttga ccgccgcctc
    12781 ggttttatct tccggcaaga ccaaattcag caagactgcc gtaatgccgc cgccggaaat
    12841 agagttttgg aacaagacgg gcaggttttt aaacacttcc ggctcaaacc ccacgcccaa
    12901 gcccaaaccg accaacgttg ccgcaatcac cgcctcgcgc ctgcggatgc cgtggccgac
    12961 caaaatccgc acgcccgcaa tcgcaatcag gccgaacatc aaaaccatcg cgccgcccaa
    13021 caccggactc ggaatcgtcg taaacgcgcg tccgacaacg gggaacagac ccaacagcac
    13081 caaaatcacg gcaatatatt tgcccacgtg gcgcgaagcc actccggtca tctgaatcac
    13141 gccgttgttt tgcgcaaacg tcgtcagcgg cagcgaaccc aaagccgtcg caatcaccga
    13201 taccaagccg tcagccaaca cgccgccgcg caggcgtttg gtgtattctt cgccttcaat
    13261 cggctggtcg gacaccattg ccgttgccgt caaatcgccg accgcctcaa acacgcccaa
    13321 caagaaaatc gcacccgccg caataaatgc gtgccagtca aaagcaaaac catatttaaa
    13381 cggtacgggc agcgtaaccg gcggcaggtt ttgcagtgcg gaaaaatcca ccttgcccaa
    13441 aaacagcgcg acaatgtagc cggcaatcag accgaccgca atgccgctca tccgcagcag
    13501 cggatttttc atgcagttga acaccagcac aatcagcagc accagcgaag ccagccccaa
    13561 gttttccatc gagccgaacg tgccgtccgc cttcgcgccg aagccgccgc cgaaatcggc
    13621 aataccgacg tgtaccaaac tcagaccaat cagcatcacg accacgccgc tgaccgtcgg
    13681 cgtaatcact tttttcaaat acggcaaaag ccacgccgag aaacacacca aaaacgcacc
    13741 ggcaaacgat acgcccaaga gcgtcgaaac catcgccccc tcactcaaac cgccctcttt
    13801 catccccgcg ccgagcgcaa tcatgacggt aacgctggat ggacagcatc cccgagccga
    13861 ccggcccgaa gcggttgacc tgcaaataag tgccgatgcc ggaagccacc atcgccatcg
    13921 acacgagata cgccgtcatt tccaccggta gttccagcgc gccgcccaca atcagcgcgg
    13981 gcgtaatcat cggcacgaaa atcgccaaaa gatgggtaac cgcgctcaag agcgcattac
    14041 cgaacggcgg cctgtcttcc aaaccgtaca ccaaatcagg cgaatccgcc tgttttttca
    14101 ttgtttcagc cattggagtc tttctatccg ttgccaaacg ttaaaaaatt ttaagaacga
    14161 aattgtaacg aatcaaagat tccttatcaa ttcaaatcct catgtgcaaa aacaccaccg
    14221 gttgtccgaa ttcaaatttc tttgaatttc cacaccggac agtccttcat ttccagagag
    14281 ccttcatgcg cttaacacat tttaatcctc aacggcgcat
//
LOCUS       DAWTHD010000064        14292 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWTHD010000064 DAWTHD010000000
VERSION     DAWTHD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.064"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            388..1263
                     /gene="prpB"
                     /locus_tag="WM704_001412"
     CDS             388..1263
                     /gene="prpB"
                     /locus_tag="WM704_001412"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFA9340821.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLMVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            1348..2496
                     /gene="prpC"
                     /locus_tag="WM704_001413"
     CDS             1348..2496
                     /gene="prpC"
                     /locus_tag="WM704_001413"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFA9340822.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLHLLHGKRLTESQVKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAECLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2648..3436
                     /locus_tag="WM704_001414"
     CDS             2648..3436
                     /locus_tag="WM704_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFA9340823.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKSLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3513..6118
                     /gene="acnD"
                     /locus_tag="WM704_001415"
                     /pseudo
     CDS             3513..6118
                     /gene="acnD"
                     /locus_tag="WM704_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            6669..7831
                     /gene="prpF"
                     /locus_tag="WM704_001416"
                     /pseudo
     CDS             6669..7831
                     /gene="prpF"
                     /locus_tag="WM704_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            8143..9342
                     /locus_tag="WM704_001417"
     CDS             8143..9342
                     /locus_tag="WM704_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFA9340824.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNEKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGSVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            9453..10073
                     /locus_tag="WM704_001418"
     CDS             9453..10073
                     /locus_tag="WM704_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HFA9340825.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(10235..10678)
                     /locus_tag="WM704_001419"
     CDS             complement(10235..10678)
                     /locus_tag="WM704_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFA9340826.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            10852..11220
                     /locus_tag="WM704_001420"
     CDS             10852..11220
                     /locus_tag="WM704_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFA9340827.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            11265..12419
                     /locus_tag="WM704_001421"
     CDS             11265..12419
                     /locus_tag="WM704_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFA9340828.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(12477..13349)
                     /locus_tag="WM704_001422"
     CDS             complement(12477..13349)
                     /locus_tag="WM704_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFA9340829.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRNGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            13434..14246
                     /locus_tag="WM704_001423"
     CDS             13434..14246
                     /locus_tag="WM704_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFA9340830.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
BASE COUNT         3244 a         3875 c         3989 g         3184 t
ORIGIN      
        1 aattaattta tattaatcat aatgttaaaa taaaataata aaattatgta attagtctcc
       61 ttaaaaatgt ttgacattcc agtcttgtgt tttagattac cgaaaaataa aactacataa
      121 tactacaaag gaacattact atgaaaccaa ttcagatgtt tttccctttt ctgaataatc
      181 cccttgtttt cttcttgtct gcggttttgc cgcataattc cgaacggtct gctgtttttc
      241 tttgattcgt tttaaatatc aataagataa tttttcccat atatttttaa tgattgggat
      301 gcccgacgcg tcggatggct gtgttttgcc gtccgaatgt gatggaagcc tgtccatact
      361 gaaaaaagtc tataaaggag aaatatgatg agtcaacact ctgccggagc acgtttccgc
      421 caagctgtga aagaatcgaa tccgcttgcc gtcgtcggtt gcgtcaatgc ttactttgca
      481 cgattggcca cccaaagcgg tttcaaagcc atctatctgt ccggcggcgg cgtggcagcc
      541 tgttcttgcg gtatccctga tttgggcatt accacaatgg aagatgtgct gatcgacgca
      601 cgacgcatta cggacaacgt ggatacgcct ctgatggtgg acatcgatgt gggttggggc
      661 ggtgcattca atattgcccg taccattcgc aactttgaac gcgccggtgt tgcagcggtt
      721 cacatcgaag atcaggtagc gcaaaaacgc tgcggccacc gtccgaacaa agccatcgtt
      781 tccaaagatg aaatggtcga ccgcatcaaa gctgccgtag atgcgcgcgt tgatgagaac
      841 ttcgtgatta tggcgcgtac cgatgcgctg gcggtagaag gtttggatgc cgctatcgaa
      901 cgcgctcagg cttgtgtcga agccggtgcg gacatgattt tccctgaagc catgaccgat
      961 ttgaaaatgt accgtcaatt tgcagatgca gtgaaagtac cagtattggc caacattacc
     1021 gagtttggtg ctacaccgct ttatacgcaa agcgagttgg ctgaaaacgg cgtgtcactg
     1081 gtgctgtatc cgttgtcatc attccgcgca gcaagcaaag ccgctttgaa tgtttacgaa
     1141 gcgattatgc gcgatggcac tcaggcggcg gtggtggaca gtatgcaaac ccgtgccgag
     1201 ctgtacgagc atctgaacta tcatgccttc gatcaaaaac tggataaatt gtttcaaaaa
     1261 tgatttaccg ctttcagacg gcctttcaac aaatccgcat cgaccgtctg aaaaataaaa
     1321 cccataaaaa tacaaaggag aaataccatg actgaaacta ctcaaacccc gaccttcaaa
     1381 cctaaaaaat ccgttgcgct ttcaggtgtt gcggccggta ataccgcttt gtgtaccgtt
     1441 ggccgtaccg gtaacgattt gagccatcgc ggttacgaca ttctaaattt ggcacaaaaa
     1501 tgcgagtttg aagaagtcgc acacttgctg attcacggcc atctgcccaa caaattcgag
     1561 ctggccgctt ataaaaccaa gctcaaatcc atgcgcggcc tgcctatccg tgtgattaaa
     1621 gttttggaaa gcctgcctgc acatacccat ccgatggacg tgatgcgtac cggcgtgtcc
     1681 atgctcggct gcgttcatcc cgaacgcgaa agccatcccg acagcgaagc gcgcgacatc
     1741 gccgacaaac tgatcgccag cttgggcagc atcctgctgt actggtatca atattcgcac
     1801 aacggcaaac gtattgaagt tgaaagcgac gaagagacca tcggcggcca cttcctgcac
     1861 ctgttgcacg gcaaacgcct gaccgaatcg caggtaaaag ccatgcacgt ttcgctgatt
     1921 ctgtatgccg aacacgagtt caacgcctcc acctttactg cccgcgtgat tgccggtacg
     1981 ggttcggata tgtattcctg catcaccggc gccatcggcg cgcttaaagg tccgaaacac
     2041 ggcggtgcga acgaagtcgc ctacgacatc caaaaacgct accgcaacgc cgacgaagcc
     2101 gacatccgcg aacgcatcgg ccgcaaagaa atcgtgatcg gtttcggcca tccggtttac
     2161 accatttccg acccgcgcaa cgtcgtgatt aaagaagtgg cgcgcggttt gagccgggaa
     2221 gcgggcgaca tgcgcctctt cgacatcgcc gaatgcttgg agagcgtgat gtgggaagag
     2281 aaaaaaatgt tcccgaatct ggactggttc tctgccgttt cctaccaaaa attgggcgta
     2341 ccgaccgcta tgttcacacc gctgttcgtg atttcccgta caaccggttg gagcgcacac
     2401 gtcctcgagc aacgcaaaga cggcaaaatc atccgtccga gcgcaaacta caccggccct
     2461 gaagatttgg cgtttgtgga gattgaagaa cgataattga agaatgcaat agcagtttgt
     2521 tctttatttc ggtatgcaaa gctgaggatt tcagacgacc ttgccttatt ggaaaggttg
     2581 tctgaaataa gtttaatcta ataggagaag ataatcctgt attggcgcaa gtaacaggat
     2641 aagaaacatg gaagatttat acataatact cgctttgggt ttggttgcga tgatcgccgg
     2701 atttatcgat gcgattgcgg gcgggggtgg tttgattacg ctgcctgcac tcttgttggc
     2761 aggtattcct cccgtgtcgg caattgccac caacaagctg caagcagccg ctgctacgtt
     2821 ttcggctacg gtttcttttg cacgcaaagg tttgattgat tggaagaaaa gtctcccgat
     2881 tgccgcagca tcgtttgcag gcggcgtggt cggtgcatta tcggtcagct tggtttccaa
     2941 agatattttg ctggcggtcg tgccggtttt gttgatattt gtcgcgctgt attttgtgtt
     3001 ttcgcccaag ctcgacggca gtaaggaagg caaagccaga atgtcttttt ttctattcgg
     3061 gctgacggtt gcaccgcttt tgggttttta cgacggtgtg ttcggaccgg gtgtcggctc
     3121 gttttttctg attgccttta ttgttttgct cggctgcaag ctgttgaacg cgatgtctta
     3181 caccaaattg gcaaacgttg cttgcaatct tggttcgcta tcggtattcc tgctgcacgg
     3241 ttcgattatt ttcccgattg tggcaacgat ggcggtcggt gcgtttgtcg gtgcgaattt
     3301 aggtgcgaga tttgccgtcc gcttcggttc gaagctgatt aagccgctgc tgattgtcat
     3361 cagcatttcg atggctgtga aattgttgat agacgagaga aatccgctgt atcagatgat
     3421 tgtttcgatg ttttaaaccc tttcagacga ctccttcaaa acatcggctg aaacccaaac
     3481 cacaagaaaa atagatccac aggagaaccg acatgactgc caaccaaagt taccgcaaac
     3541 cgctgcccgg tacggatttg gaatactacg acgcgcgtgc ggcgtgtgag gacatcaaac
     3601 ccggctctta cgacaagctg ccgtacacga gccgcatttt gtctgagaac ttggtcaacc
     3661 gcgcggacaa agtcgatttg ccgatgctgc aaagctggct gggccagttg atagaaggga
     3721 agcaggaaat cgattttccg tggtatcccg cgcgggtggt gtgccacgat attctggggc
     3781 agaccgcgct ggtggatttg gcaggcttgc gcgatgcgat tgccgaaaaa ggcggcgatc
     3841 ctgccaaagt gaatccggtg gtgcagacgc agcttatcgt cgaccactct ttggcggtgg
     3901 agtgcggcgg ttacgatcct gatgccttcc gcaaaaaccg cgaaatcgaa gacagacgta
     3961 acgaagaccg tttccacttc atcaactgga caaaaaccgc atttgaaaat gtggacgtga
     4021 ttccggcggg caacggcatc atgcaccaaa tcaatctgga aaaaatgtcg cccgtcgtcc
     4081 aagtcaaaaa cggcgtggct ttccccgata cctgcgtcgg cacggactcg catacgccgc
     4141 acgttgatgc gctgggcgtg atttccgtgg cgtgggcggt ttggaagcgg aaaccgtgat
     4201 gctgggacgc gcgtccatga tgcgcctgcc cgatattgtc ggcgtggagc tgacgggcaa
     4261 acggcaggcg ggcattactg ccacagacat cgtgttggca ctcaccgaat tcttgcgtaa
     4321 agagcgcgtg gtcggggcgt ttgtcgaatt tttcggcgag ggcgcgagaa gcctgtctat
     4381 cggcgaccgc gcgaccattt ccaacatgac gccggagttc ggcgcgactg ccgccatgtt
     4441 cgccatcgac gcgcaaacta ttgattattt gaaactgacc ggacgtgacg acgcgcaggt
     4501 gaaattggtg gaaacctacg ccaaaaccgc aggcttatgg gcaggtggct tgaaaaccgc
     4561 cgtttatccg cgcgttttga aatttgattt gagcagcgta acgcgcaata tggcaggccc
     4621 gagcaacccg cacgcgcgtt ttgccaccgc cgatttggcg gcgaaagggc tggcgaagcc
     4681 ttacgaagag ccttcagacg gccaaatgcc tgacggtgca gtgattattg ccgcgattac
     4741 ttcgtgtacc aatacttcca acccgcgcaa cgttgtcgcc gccgcactgt tggcacgcaa
     4801 tgccaaccgc ctcggcttga aacgcaaacc ttgggtgaaa tcttcgtttg ccccgggttc
     4861 aaaagtagcc ggaatctatt tgaaagaagc aggcttgttg cccgaaatgg aaaaactcgg
     4921 cttcggtatc gtcgccttcg catgtaccac ctgtaacggc atgagcggcg cgctcgaccc
     4981 gaaaatccaa caagaaatca tcgaccgcga tttgtacgcc accgtcgtat tgtcaggcaa
     5041 ccgcaacttc gacggccgta tccatccgta tgcgaaacag gctttcctcg cttcgcctcc
     5101 tttggtcgtt gcctacgcat tggcaggtag catccgtttc gatattgaaa acgacgtact
     5161 cggcgttgca gacggccgcg aaatccgcct gaaagatatc tggccgacag acgaagaaat
     5221 cgatgccatc gttgccgaat atgtgaaacc gcaacaattc cgcgacattt atatcccgat
     5281 gtccgacacc ggcacagcgc aaaaagcacc aagcccgctg tacgactggc gaccgatgtc
     5341 cacctacatc cgccgtccgc cctattggga aggcgcactg gcaggggaac gtacattaag
     5401 aggtatgcgt ccgccggcga ttttgcccga caacatcacc accgaccaca tctcgccatc
     5461 caatgcgatt ttggccggca gtgccgcagg tgaatatttg gcgaaaatgg gtttgcctga
     5521 agaagacttc aactcttacg caacccaccg cggcgaccac ttgaccgccc aacgcgcaac
     5581 cttcgccaat ccgaaactgt ttaacgaaat ggtgagaaac gaagacggca gcgtacgcca
     5641 aggttcgttg gcacgcgttg aaccagaagg ccaaaccatg cgcatgtggg aagccatcga
     5701 aacctatatg aaccgcaaac agccgcttat catcattgcc ggtgcggact atggtcaagg
     5761 ctcaagccgc gactgggcgg cgaagggcgt gcggctggcg ggtgtggaag ccatcgccgc
     5821 cgaaggtttc gagcgcatcc accgcaccaa cctcatcggc atgggcgtct tgccgctgca
     5881 attcaaaccc ggcaccaacc gccataccct gcaactggac ggtacggaaa cctacgacgt
     5941 tgtcggcgaa cgcacaccgc gctgcggcct gaccctcgtg attcaccgta aaaacggaga
     6001 aaccgtcgaa gttccggtca cctgccgccc cgataccgca gaagaagtat tggtatatga
     6061 agccggcggc gtgttgcaac ggtttgcaca ggactttttg gaagggaacg cggcttagag
     6121 ttcgtctgaa aagcaagacg tagcgtgggt cgggttctca ttcacgtaat tcccgatatg
     6181 gcaggcattt actgtaaatc gtcattcccg cgcaggcggg aatccagaaa gtggaattga
     6241 ggaaaccttt ttatccgatg agtttctgtg cggataaatc tggatttccg cctacgcggg
     6301 aatgacgggg tttaataatc taccgtatca caacacagca gccgtagatt ggggcgaacc
     6361 ccgacagttt gcggaatcaa acggctttgg tcggagtggt agcctaatgt acttctggaa
     6421 agtgggtgta gcgtgggctt tgcccacgaa ataaaggctg aattgacatg gtatagcgga
     6481 ttaacaaaaa tcaggacaag gcggcgggcc gcagacagta cggatagtac ggaaccgatc
     6541 cgcccggtgc ttgggcatct tagggaaccg ttccctttga gccggggcgg ggcaacgccg
     6601 taccggttta aattaatcca ctataaaaat cgtgggcaga gcccacgcta cataaggaga
     6661 accctaaaat gccgcaaatc aaaatccccg ccgtttacta ccgcggcggt acatctaaag
     6721 gcattttctt caaacgcacc gatctgccgg cagctgcgca ggaagcaggc ggggcgcgcg
     6781 acaaaatcct cttgcgcgta ctcggcagcc ccgaccctta cggcaaacag attgacggtt
     6841 tgggcaacgc cagttcgtcc accagcaaag ctgtgatttc ggacaaatcc gaacgcgccg
     6901 atcacgatgt cgattacttg ttcggacaag tttccatcga caaaccattt gtcgattgga
     6961 gcggcaactg cggcaacctt accgccgccg tgggcgcatt cgccatcgaa cagggcttgg
     7021 tcgataaatc caaaatccct tcagacggca tctgcacggt caaaatctgg cagaaaaaca
     7081 tcggcaaaac cattattgcc catgtgccga tgcaaaacgg agcagtgttg gaaacaggcg
     7141 atttcgagct tgacggcgta attttccggc agccgaagta caaatcgaat ttttagatcc
     7201 agccgacggc gaaggcagta tgttcccaac cggcaatttg gtcgacgaaa ttgatgtgcc
     7261 gaatataggt cgtctgaaag ccacgctcat caacgcgggc attccgaccg ttttcctgaa
     7321 cgccgccgac ttgggctaca cgggcaaaga gttgcaggac gacatcaaca acgatgccgc
     7381 cgcgctggaa aaatttgaaa ccatccgcgc atatggcgcg ctgaaaatgg gtttgatcag
     7441 cgacgtatcc gaagccgccg cccgcgcgca cacgccgaaa cccgccttcg tcgcgcccgc
     7501 cgccgattac accgcctcca gcggcaaaac cgtaaacgcc gccgacatcg atttgccggt
     7561 acgcgccctg agcatgggca aactgcacca cgctatgatg ggcatcgcct cggtcgccat
     7621 cgccgccgcc gtgctcggta cgctggtcaa ccttgccgca ggcggcggaa cgcgtaaaga
     7681 agtgcgcttc gggcatccgt caggtacgct gcgtgtcggt gctgccgccg aatgtcagga
     7741 cggacaatgg acggccgcca aagcggtcat gagccgcagc gcacgcgtga ttatggaagg
     7801 ttgggtgcgc gttcccgatg attgtttttg aaatcatgta ggacgcacat tgctgtccgc
     7861 tgtttcagac ggcatttgat taaggattac cataaagccg aataacctta ccgccgtcat
     7921 tcccacgcag gcgggaatcc gcagccgata ttctgtttcc tgatttttat cagagcctgc
     7981 gtgtgagcaa atatggattc ccgcctgcgt gggaatgacg ctaggaaata cggcatacgc
     8041 tttgcccaaa gaggccgtct gaaacacact ccgcccaacg cccatccttt ttcagacggc
     8101 cccgcaccaa aaaaacaacc acaaactaca aggagaaaca tcatgtccga ccaactcatt
     8161 cttgtcctga actgcgtcag ttcatcgctc aaaggcgccg ttatcgaccg caaaagcggc
     8221 agcgtcgtcc taagctgcct cggggaacgc ctgactacgc ccgaagccgt cattaccttc
     8281 aacaaagacg gcaacaaacg ccaagttccc ctgagcggcc gcaactgcca cgccggcgcg
     8341 gtgggtatgc tgttgaacga actggaaaaa cacggactgc acgaccgcat caaagccatc
     8401 ggccgccgca tcgcccacgg cggcgaaaaa tatcacgagt ccgtcctcat cgaccaagac
     8461 gtccttgacg aactgaaagc ctgcatcccg ttcgccccgc tgcacaaccc cgccaacatt
     8521 agcggcatcc tcgccgcgca ggaacacttt cccggcctgc ccaacgtcgg cgtgatggac
     8581 acctcgttcc accaaaccat gccggagcgg gcctacactt atgccgtgcc gcgcgaattg
     8641 cgcaaaaaat acgccttccg ccgctacggt ttccacggta ccggtatgcg ttacgtcgcc
     8701 cctgaagccg cacgcatctt gggcaaacct ctggaagaca tccgcatgat tattgcccac
     8761 ttaggcaacg gcgcatctat taccgccgtc aaaaacggca aatccgtcga taccggtatg
     8821 ggtttcacgc cgatcgaagg tttggtaatg ggtacacgtt gcggcgacac cgatccgggc
     8881 gtatacagct atccgacttt ccacgcaggg atggatgttg cccaagttga tgaaatgctg
     8941 aacgaaaaat caggtttccc cggtatttcc gaacttccca acgactgccg caccctcgaa
     9001 atcgccgccg acgaaggccg cgaaggcgcg cgcctcgccc tcgaagtcat gacctgccgc
     9061 ctcgccaaat acatcgcttc gatggctgtg gcctgcggca gtgttgacgc actcgtgttc
     9121 accggcggta tcggcgaaaa ctcgcgtaat atccgtgcca aaaccgtttc ctatcttgat
     9181 ttcttgggtt tgcacatcga caccaaagcc aatatggaaa aacgctacgg caattcgggc
     9241 attatcagcc cgaccgattc ttctccggct gttttggtcg tcccgaccaa tgaagaactg
     9301 atgattgcct gcgacactgc cgaacttgcc ggcatcttgt agccaaaaaa gggggcgagt
     9361 ccgcaaaaat gccgtctgaa aggttttcag acggcatttt gtttggcgtt tgggcgtaca
     9421 atacggaaaa catgacgata aggaaacaaa ctatggcaca atttttcgcc gttcatcccg
     9481 acaatcccca agaacgcctc atcaagcagg cagtcgaaat catcaatcaa ggcggcgtgg
     9541 tggtttatcc gaccgattcc tgttacgcct tgggttgcaa actcggcgat aaggcggcga
     9601 tggaacgcat actctccatc cgaaaaatcg atttgaaaca ccacctgacc ctgatgtgcg
     9661 ccgatttgag cgagttgggc acatacgcca aaatcgacaa cgtacagttt cgtcagctta
     9721 aagccgccac gcccgggcct tataccttta tcttgcaggc gacgaaggat gcgccggcgc
     9781 gcgccctgca cccgaaacgc aaaaccatcg ggctgcgtat tcccgataat gccgttgcac
     9841 aagccctgct ggaggagttg ggcgggccgc ttttaagctg caccctgatg ctgcccgaag
     9901 acggcgaacc attgaccgac ccttatgaaa tccgcgagcg tttggaacac gccgtcgatt
     9961 tggtaattga cggcggctgg tgcggaaccg atccgactac cgtcatcgac atgaccgacg
    10021 gcacggaact ggtgcgccgg ggtttcggcg atacggcggt gttcggtttg tagggaaacc
    10081 gatgccgtct gaagcatcag ccgttcagac ggcatcgcgc gccttgccgg cggcagcccg
    10141 aaatgccggc gcgtatcgcg cccggtcgga atatccgttt gaaacggcaa acaaaatgcc
    10201 gtccgaacgc gggtttgggc ggcattttga tgcgttatcg caccgcaatc gggattttcg
    10261 attcataaag caggtcgtag gtcggcttgt tgagcaggtc ttgcagcgtg aaaccgtcca
    10321 gatacgtgaa aaacgacttc attgcaccgc cgagtatgcc cgtcagccgg caggacggcg
    10381 taatcaggca ttcgttgttc gggcccatac actcgaccag ctgcatcggt tcgaggtggc
    10441 ggacgaccgc gccgatattg atgcgttcgg gcggcgcggc cagcctcagc ccgccgcctt
    10501 tcccgcgtac gctgtgcaag aacccgcctt tgaccagcgc ggtaaccact ttcatcaaat
    10561 ggcttttgga aatgccgtag gtcgaggcga tggtggcgat attgaccagc gcgtcgtcgt
    10621 tgacggcggt gtagatgagg acgcgcagcc cgtagtcggt atgttgggtc agatacatga
    10681 ttttctcggt atggatcgtt attcttatcg gtacggttta agtttcacgg aaaatacctt
    10741 aatggttgaa accctgtccg tcggggcggt agaatgtagc ctgtctgcgg cggtatgccg
    10801 tctgaaacat ccgcgctacc gtttgataat ttgttattgt aacctaaatt tatgaaaccg
    10861 ttgaaacgac attccgccct tgtcgggctt tcgcgcgaac accaccactc gctttccctg
    10921 tgcgtgcgta tgttgcggac gcccggggca gaccatcgtg ccgaactcga gctgcatttt
    10981 gccgaactgg aaacccattt tcgcgaagaa gaagccaagt ttgccccaat ttggcagaat
    11041 gtcgcgcctg agttgaaaac ccgttttgaa ggcgatcacg ccaaactgcg gcagatgatg
    11101 gcaagccccg aatgcggtaa cgcggcgtgg aataccgctt ttgccacaac cctgcgcgac
    11161 cacgcgcgct ttgaagaacg cgagctgttt cccgccgtcg aaccgttttt gccggcatga
    11221 ttccgttttg cggcaaatat attaatgata aacaaggaac acacatgaaa tttaccaaac
    11281 atcccgtctg ggcaatggcg ttccgcccgt tttattcact ggcggcactg tacggcgcat
    11341 tgtccgtatt gctgtggggt ttcggctaca cgggaacgca cgagctgtcc ggtttctatt
    11401 ggcacgcgca tgagatgatt tggggttatg ccggtctcgt cgtcatcgcc ttcctgctga
    11461 ccgccgtcgc cacttggacg ggacagccgc ccacgagggg cggcgttctg gtcggcttga
    11521 ccgccttttg gctggctgcg cggattgccg cctttatccc gggttggggt gcggcggcaa
    11581 gcggcatact cggtacgctg tttttctggt acggcgcggt gtgcatggct ttgcccgtta
    11641 tccgttcgca aaaccggcgc aactatgtcg ccgtattcgc aatatttgtg ctgggcggta
    11701 cgcatgcggc gttccacgtc cagctgcaca acggcaacct aggcggactc ttgagcggat
    11761 tgcagtcggg cctggttatg gtgtcgggct ttatcggcct gattgggatg aggattattt
    11821 cgttttttac gtccaaacgg ttgaacgtgc cgcagattcc cagtccgaaa tgggtggcgc
    11881 aggcttcgct gtggctaccc atgctgaccg ccatactgat ggcgcacggc gtgatgcctt
    11941 ggctgtcggc ggctttcgcg tttgcggcgg gcgtgatttt taccgtacag gtgtaccgct
    12001 ggtggtataa acccgtattg aaagaaccga tgctgtggat tctgtttgcc ggctatctgt
    12061 ttaccggatt ggggctgatt gcggtcggcg cgtcttattt caaacctgcc ttcctcaatc
    12121 tgggcgtaca tctgatcggg gtcggcggta tcggcgtgct gactttgggc atgatggcgc
    12181 gtaccgcgct cggtcatacg ggcaattcga tttatccgcc gcccaaagcc gttcccgttg
    12241 cgttttggct gatgatggcg gcaaccgccg tccgtatggt tgccgtattt tcttccggca
    12301 ctgcctacac gcacagcatc cgcacgtctt cggttttgtt tgcactcgcg ctgctggtgt
    12361 atgcgtggaa atacattccg tggctgatcc gtccgcgttc ggacggcagg cccggttgag
    12421 taaaaccgcc gcagatttcg ggtctgcggc ggtttgcttt tcagacggca tggcggtcag
    12481 ttgccgtcca gccagcggtc gcgcgtggtt ttggcttctt caaaatagcg gtacagggct
    12541 tcgcggtcgt cggcggtcag gatgtctgcc aaaacgtcca actgtttgcc caagccttga
    12601 accagttgca gcaggctgtc tttgttggca aggcagatgt ccgcccacac ggcgggatgg
    12661 ccggaggcga tgcgggtgaa gtcccgaaag cccgtggcgg cgaatttcag atattcctgc
    12721 ccgtcggggt ggtcgagaat ctggtggaca taggcgaagg cggtcaggtg gggcatatgg
    12781 gagacggcgg cgaaaaccgc gtcgtggtgt tgcgcgtcca tcgtgaaaat gtccgcaccg
    12841 accgcgcgcc acaggttttc taccaatgcg atgccgtctg aatcttcgcc gccgtggggc
    12901 gtgatgatga gttttctgtg gcggaacagc ccgaactgcg cggcttgcgc accgtttctg
    12961 tccgaaccgg caatcgggtg tgcggcgatg cagcggtgca ggcggtcggg caggcagcgg
    13021 cggaaggctt cgatgaccga agatttggtg ctgccgacat cggaaatcca agtgtgttcc
    13081 ggcaaaacgg ggcgcagcgc ggttaaaacg gcgggaacgg tggcgacggg cgtggcaatc
    13141 agtaccaagt ccgcaccgcc gatgctgtcc gcgtcgatga cgacggaagc ccggttaatc
    13201 acgccgcgtt ccaatgcacg ttcgaggttg tcgcggtcgg tgtcgatacc ggtaacggtg
    13261 cggacgagtc cctgcctttt gaggtcgaga acgaacgaac cgccgatcag ccctacaccg
    13321 atgagggcaa tatggttcaa aatgggcatt tgtgtaaacg gttttcgcaa agtgccgcca
    13381 tggtagccta tcggcggaat atgccgcaag gtcggcagga aaaaggagaa gaaatggaca
    13441 aaatcagagt tgccgccgtg cagatggtgt cgggcgtgtc gccggaaacc aacgtcgccg
    13501 ccatgaaacg cctggtcgca cgggcggcgg agcagggtgc ggattgggtg ctgctgcccg
    13561 aatattgggt gctgatgggc gcaaacgata ccggcaaact cgcacttgcc gagcctttgg
    13621 gcggcggacg ctttcagacg gcattgagcg aaacggcgaa agaatgcggc gtggtgctgt
    13681 tcggcggaac cgtgccgctg caaagccccg aagcgggcaa ggtcatgaat actttattgg
    13741 tgtacgggtg cgacggcgta aggacggggc tgtaccacaa aatgcacctc ttcggttttt
    13801 ccggtttggg cgaacgctat gccgaagccg ataccatccg cgcagggcgg gaagtgccgc
    13861 acttgtcggc agaaggcatg ccggtggcgg caggtatttg ttacgatgtc cgctttcccg
    13921 aatttttccg ccgccaactg ccgtttgacg tattgatgct gccggcggca tttacccata
    13981 cgacgggcaa ggcgcactgg gagctgctgc tgcgcgcgcg tgccgtcgaa aaccaatgtt
    14041 acgtcgtggc ggcggcacag ggcggtttgc acgaaaacgg acggcgcacg ttcggacaca
    14101 gtatgattgt cgatccgtgg ggcgacgtgt tggacgtatt acccgaaggc gaaggcatcg
    14161 ttacggcaga catcgatgcc aaccgcctga acagcgtccg caaccgcctg cccgccttga
    14221 aacaccgggt tttggatgcc gtctgaaagt tcagacggca tcggtgtcgg ggaatcagcg
    14281 ggccgtagca ac
//
LOCUS       DAWTHD010000065        13172 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWTHD010000065 DAWTHD010000000
VERSION     DAWTHD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.065"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..232)
                     /locus_tag="WM704_001424"
     CDS             complement(<1..232)
                     /locus_tag="WM704_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9340831.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAEPDSIVYTDCYRRYDV
                     LDAGEFSHFRINHSTHFAERQNHINGIGNFWNR"
     gene            complement(340..534)
                     /locus_tag="WM704_001425"
     CDS             complement(340..534)
                     /locus_tag="WM704_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFA9340832.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(524..2605)
                     /locus_tag="WM704_001426"
     CDS             complement(524..2605)
                     /locus_tag="WM704_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFA9340833.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            3128..3508
                     /locus_tag="WM704_001427"
     CDS             3128..3508
                     /locus_tag="WM704_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFA9340834.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3644..4116)
                     /locus_tag="WM704_001428"
                     /pseudo
     CDS             complement(3644..4116)
                     /locus_tag="WM704_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244189.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
     gene            4464..5852
                     /locus_tag="WM704_001429"
     CDS             4464..5852
                     /locus_tag="WM704_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFA9340835.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIVKP
                     ASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNPTVRKISFTGSTEVGA
                     KIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKFRNSGQTCVCTNRVYV
                     QSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKVEQHIADALSKGAVCL
                     TGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFETEAEVIEAANNTEYG
                     LAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVKRSGLGREGSKYGADE
                     YLELKYLCIDAG"
     gene            5963..6748
                     /locus_tag="WM704_001430"
     CDS             5963..6748
                     /locus_tag="WM704_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFA9340836.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            7116..8672
                     /locus_tag="WM704_001431"
     CDS             7116..8672
                     /locus_tag="WM704_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFA9340837.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMVVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8689..9435
                     /gene="surE"
                     /locus_tag="WM704_001432"
     CDS             8689..9435
                     /gene="surE"
                     /locus_tag="WM704_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFA9340838.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFCYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9537..10550
                     /locus_tag="WM704_001433"
     CDS             9537..10550
                     /locus_tag="WM704_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFA9340839.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLREYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10948..11430)
                     /locus_tag="WM704_001434"
     CDS             complement(10948..11430)
                     /locus_tag="WM704_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFA9340840.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11583..11744)
                     /locus_tag="WM704_001435"
     CDS             complement(11583..11744)
                     /locus_tag="WM704_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340841.1"
                     /translation="MSEQPEKHHASPIEDERKKPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11818..12279)
                     /gene="recX"
                     /locus_tag="WM704_001436"
     CDS             complement(11818..12279)
                     /gene="recX"
                     /locus_tag="WM704_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFA9340842.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12352..13062)
                     /locus_tag="WM704_001437"
     CDS             complement(12352..13062)
                     /locus_tag="WM704_001437"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFA9340843.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
BASE COUNT         2991 a         3299 c         3734 g         3148 t
ORIGIN      
        1 cccggttcca aaagttccca attccattaa tatggttttg tcgttcggca aaatgtgtgc
       61 tgtgattgat acggaaatgg ctaaattcgc ccgcatccaa tacatcatag cgacgataac
      121 aatccgtata aacaatgctg tcaggctctg ctgctgttct tgcagttacg cctgcgacaa
      181 acagttcaat gagtttattt tgtttatacc ggcttagacg gctttttctc atagggataa
      241 ttctaactta atttgaattt ccctagttat ctaggacagc gccttattat aaaaataccg
      301 tctgaagtgg cgttcagacg gcatttgttt caagcggggc taacagcagc gtccgccgtt
      361 tgcgccgcag cggcgtttgc ggcaatagtc ttgaaattgc agcttgttca tcacgggggc
      421 gttgggatta tgtttacgtt gttgtgcaac gtagttttca taatcgggca cgcctgccat
      481 caagtttgcc gtcagcttga tggttttcca ccaagacgcg agcttatgct tcactttgtt
      541 cctccggttg tttgccgtcg cggtacaccg ccgggatttc tttggcggtc ggccagccga
      601 ctttgcgtgc tttgagggcg gtacgcaaac cgtacgcggc aacagtcacg acaaccgaca
      661 agaagaggat ggtcagaccg gcattaatct tgtcgttgaa gatgatttgc gccatttcgc
      721 cgatgtcttt agcgggcgca aggacttcgt ttttagccaa tgcgtcgctg tatttgcctg
      781 cgtgggcaag gaagctggcg cgcgggtcgt tgtggaacag tttttgcaga ccggcgtagc
      841 aggttacaag cagtacgccg acggcaggaa cgagtgccac ccagacataa cggtcgcgtt
      901 tcatcttaat cagcacaacg gcacacataa tcaaggctac gcctgccagc atttggttgg
      961 cgatgccgaa taaaggccag agcgagttga tgccgcccag cgggtcggtt acgccggtgt
     1021 agaggaagta gccccacaat gccacggcga agaaggtcgc aatcaggttg gcggggatgg
     1081 agtcggtgtt gccgaaaggt ttgtagaaga tgctgcccaa gtcttgaatc ataaaacgtg
     1141 cgacgcgcgt accggcatcg acggcggtca ggatgaacaa ggcttcaaac aacagggcga
     1201 agtgatacca gaatgccatc atcgcctcgc ccggaatcag acggctcata atgtgcgcca
     1261 taccgactgc gagggtaggc gcaccgcctg tgcgggacag gatggtgttt tcgcccactt
     1321 ccttagcggt gtgcaacagg gttgcggcat cgacagggaa gttcagcttg gtggtaatta
     1381 cttcggcggc ggtgttggca tccgtaccga tcagtgcggc ggggctgttc atcgcgaaat
     1441 acacgccggg atcgagcgat gcggcggcgg caagcgccat aatcgccacg aaactttcca
     1501 tcaacatacc gccgtaaccg atcatacgga cgtgggtttc gttttccagc attttcggcg
     1561 tggtgccgga agaaatcagt gcgtggaagc ccgaaaccgc accgcaggcg atggtaatga
     1621 acaagaacgg gaacaatgcg cctgagaata ccgggcccga accgtcgata aagtgggtaa
     1681 ccgcaggcat ttgcaaagcg ggattgacga tgacgatacc caaagccaag gccgcaatcg
     1741 taccgatttt caggaaggtg gagagatagt cgcgcggggt cagcaagagc cagaccggca
     1801 aaaccgaagc cacgaaaccg taaatcataa tcgcccaagt gagctggatg ccgtcaaggt
     1861 caaaccaatg cccgatggag cttttcgcca catcttcgcc gtaaattacc gccaacatca
     1921 gcaaaataaa gccgacgatg gaaatctcgc cgattttgcc cggacggata tggcgcgtgt
     1981 aaatacccat aaacagcgca atcggcatcg ttgcggcaat ggtgaacgta ccccaagggc
     2041 tgtgaaccaa tgcttttacg acaatcaacg ccaaaaccgc cataatgatg accataatca
     2101 tcaaaatacc gatggaggca atcacgccgg ggacagtgcc gagttcctgt ttcacaatat
     2161 cgcccaaaga cttaccgtcg cggcgcatag agacgaacaa gaccgtcata tcctgtaccg
     2221 cgccggcaaa taccacgccg aagataatcc acaaagtacc gggtagataa cccatttgcg
     2281 ccgccaaaac cggaccgacc aaagggcccg cgccggcaat cgcggcaaag tggtgtccga
     2341 acaatacgcc tttgtgcgtc ggaacgtagt ccaagccgtc gttgtgccgc tcggcgggcg
     2401 tgagacggtt cggatcgagc cgcatcacgc ggttggcgat gtagaggctg taaaaacggt
     2461 aggcgataca gtaaacggat atggcggcgg taaccatcca taccgcgctg acctgttcgc
     2521 ctcggctgag ggccagagtg gtaaaggatg ctaagccgac cagtaccact atgccccaaa
     2581 tgaggaaggt tttgagtgat ttcatcgaat aaatccttat ctcacactgt cggaatatgt
     2641 cggagcggac aggcaacggt tggctacccg taatccgatg ccgacacaaa ccggagtttg
     2701 aatgattgac gaagtatgaa acagtgcgct tgtcgggact tgtgccttat gcccgcatca
     2761 aacagtgcag gaatgtacgc gcacaaacgt taaacgccga atagaatttt aacgcaaatt
     2821 agcacactga tagcggtttt tacttggaat ttggaaaaat ttacattcct ccgggcgggc
     2881 aggcaggttc agacggcatc gtcaggcaaa aggcggtgtc ggaagacggg taaagaaggg
     2941 gcgcgcaatc cggattattg atttatcgtg tttccttttc ggttgaacct cagccctttg
     3001 gggcggtaaa atcagacttt atttgggagg ggcgcaaccc cttccgaatc aggacggcac
     3061 atagggcggt gctttaatgt gtcgtcctgt gtgttgaaac atattttctt tttattgggg
     3121 agcttttatg aatatcaggt ttttcgcgct gaccgtatcg gttttgtctt tggcggcctg
     3181 tgccgtgccg gaagcgtatg atggcggcgg acgcgggtat atgccgcctg ttcaaaacca
     3241 agccggcccg gacgattttc gagcgttttc atgcgagaac ggtttgtctg tgcgcgtccg
     3301 caatttggac ggcggcaaaa tcgcgttgcg gctggacggc aggcgtgccg tcctctcttc
     3361 cgacgttgcc gcatccggcg aacgctatac cgccgaacac ggtttgttcg gaaacggaac
     3421 cgagtggcac cagaaaggcg gcgaagcctt tttcggcttt accgatgcct acggcaattc
     3481 ggtcgaaact tcctgccgcg cccgttaaac ggggcagcct gtttgaaccg ccgtccggat
     3541 ttttcgggcg gttttttatg ccggttttgt tttggccgtc ggctggttcc ggcggttttc
     3601 gggcgtggcg gattattttt cgtttcgttc tgggtttatc ctgtcggcag cggctcaatg
     3661 ccaactttaa acctgctccg atttcttcag ggctgttatc caatgataaa attacatcgt
     3721 ctgcatcaat ggcatcccac gcttccggct tgacatggcg gctcgggctg attttcaggc
     3781 agccgttgtg cagccaaata tccccgctca tcatgttttt aaatagggcg cgtctggttt
     3841 tatagcccaa gttcccgcat aggttggcaa cccaatcctc atagcgttgc cgaaattttt
     3901 cggtatcaaa aaaatcttgg tcttccgaac tgtcataaac gaaagtcctg ctgtttgcca
     3961 acgcttgcaa gaccgtcgtg cctaaagttt cattgtcggt atccaatggc aggatatggg
     4021 gggatatagg tggtctattg ccgttaaccc caaacctgaa catgtttgaa caatcagagc
     4081 ttcttcatta gcataaaaaa ctgcccaata attgatcctg tttaaaaatc attatttaat
     4141 ctccgtaatt ttgactgtaa tgttttgact tttgccatac tctaccacgc gttgtaactg
     4201 caatctttgc ttcttatata ttgtacccta tcaaagggct gcattacttt tctttctaaa
     4261 gattactatc gcataaaaag tatttttctt aaacgacagg gatgcgcccg tgccatattc
     4321 aaacaccgcc cggatgttgc gctgcccgat cggatgcttc agacggcatc ggaagggttt
     4381 gcagttttgg aatatgaaga tgataatgtc cgcgagattg acggcatttg aaagcggtta
     4441 tcccgtatac cggaggatac gaaatgaacg aatattccca attaatcaag catcccgata
     4501 tttccctttc cccgatttca gacggcatcg gggtcggcaa tccggcgacg ggcgaaattt
     4561 tggcgtatgt ccgcaacacg ggttcggaca agctgaaaaa cctgattcaa aaggcggcgg
     4621 cagcgcaaaa attatgggcg gcgaaaaccg ccttggaacg cgccgacata ttgtggcgtt
     4681 ggtatttttt agttaaagaa aacaaagaag cactggcgcg tctcatgacg atggagcagg
     4741 gcaaaagcct gaccgaggcg cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt
     4801 ttgccgaaga ggcgcggcgg attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa
     4861 aactggttgt gttgaaacag cccgtcggcg ttaccgctgc gattacgccg tggaatttcc
     4921 cgtccgcgat gatagtcaaa cctgcatcgc tcacgccttt gagtgcgtat gcgttggctg
     4981 tactggccta cgaagcgggc gtgccgcagg atttgcttcc cgtcgtcagc ggccgcgctt
     5041 cggaaatcag ccatgaattt gccacgaacc cgaccgtgcg caaaatcagc ttcaccggct
     5101 cgaccgaagt cggcgcgaaa atttttgccg gcagcgcggc ggacattaaa aaactcagtt
     5161 tggaactggg cggcaacgcg ccgtttatcg tgtttgacga tgccgatttg gacaaagccg
     5221 tcgaaggcgc gctggcgagc aagttccgca acagcggtca gacctgcgtc tgcaccaacc
     5281 gcgtttacgt ccaatcgggc atttacgacg tattttgccg caaattgagt gaaaaggcag
     5341 ccgcgctcaa attgggcgac ggcttggacg aaggtgtgaa ccaaggaccg ctgattgagg
     5401 aaaaagcggt agaaaaagtc gaacagcaca tcgccgacgc actctccaaa ggcgcggtct
     5461 gtctgaccgg cggcaaacgc agcgcgttgg gcggaacgtt ttttgagccg actgtcttaa
     5521 gcggcgttac ggcgcaaatg gcggtagctc gcgaggaaac cttcgggcct ttgtgtccgg
     5581 tgttccgttt tgaaaccgaa gccgaagtca tcgaggctgc gaacaatacg gaatacggtt
     5641 tggcagctta ccttttcacc tccgacaccg cccgtcaatg gcgcgtcggc gaagtcttgg
     5701 aatacggcat ggtcggcatc aatacgggct taatcagtaa tgaagcggca ccgttcggcg
     5761 gggtgaaacg cagcggtttg ggacgtgaag gcagcaaata cggtgcggac gaatatctag
     5821 aattgaaata tttgtgcatt gatgccgggt gacggatgcc gtctgaatgg cagggtttca
     5881 gacggtgtcg cattttaagc agtctctatc tgttgtacaa tgcgccctgt ttttacggtt
     5941 atttttgatt tgaatgtaag atatgatgga aaacggaaaa acattcccga gatggcgttt
     6001 tgccttgaaa agtgcgggct ggcacctctt aatcagctta ttggttgcag ggctggcggc
     6061 cttgctggtt tttaaggttt ggtatcctta tccttatgcc gagctgacgg gcggtctgtc
     6121 gctttatcag ctggtggtgg cggtcgatat cgtatgcggc ccgctgctga ctttaatttt
     6181 ggcaagcccg aagaaaaaga caaaggcacg catggtcgat ttttccatgg tcggcatcat
     6241 ccagctggcg gctttggcgt acggtttgca cagcgtttcg ctggcgcgtc ccgtggtgga
     6301 agcgtttgaa caggaccgca tgaccattgt tacggcggcg gaagtcgtgg tcgaagattt
     6361 gcacaaagcc cccgaagggc tgcaaagcct gtcgtggttc ggcatccgcc gcattgcatt
     6421 gaaagaacct gaggatgcgg atgagaagaa caagacgctg gatttgtccc tgaaaggtat
     6481 cgagccgagt atgcgtcccg accggtggct gccgtattcc gacaaggaag cagaaaaaat
     6541 ccgcaaacat ctgaaaccgc tgaaagtctt ggcggatgcg agaaaaacga cggttgcgga
     6601 cattctgaaa caggcaggtc tcgccgaagg ggaggagctg tattacctgc cgtttaccag
     6661 cagcaggcag aaagagtgga tagtcattac cgataaagag ggcaacacca aaggctacgc
     6721 gccgatagac ggcttcatca tcccttaacc attgggattc cgcccgcact cgaacatccg
     6781 ttcttcgcgg cggtagaatc agactgtatt tgagaggggc gcaacccctc aatcagggca
     6841 acacataggg agacgcttta tgtgccgtcc gtgtattgaa acatttcaaa ataagcccat
     6901 gccgtccgaa catcctttca gacggcatgg cgttttgcca ttgccccgat aagctgttaa
     6961 actgtttaaa ttatttcgac cgaaggtaca cgcacatgaa agaccccgag cagagcagta
     7021 agcccgcccg ccgttttttg tgcgtaccgt ctgttcaggc agcttgagcg tttcaggctg
     7081 cctttttgcg tttgaacttg aggaacacga aaatcatgga tttcagttgg ttggcagaac
     7141 cgcatacctg gataggtttt gccacgcttt tggtgttgga agtcgtattg gggatagaca
     7201 accttgtctt tgtggcgatt ttggcaaaca aggtccagcc cgcacggcgc gaccgcgcac
     7261 ggattatcgg gctggggctg gcagtcgtca tccgcatcat tatgcttgct tttatggcgc
     7321 acatcatcac gctgaccgag ccgctgttcc aaatcggcgg cctcgccgtt tccggcaagg
     7381 acatgattat gctcgcgggc ggtattttcc tgctttacaa agccaccacc gaactgcatg
     7441 aacgcctcga agggcacaac cgttttaccg ttgccgacaa ccaaaaaaaa cacgcgccgt
     7501 tttggggcgt ggtcgcgcaa atcctgatac tggatgccgt gttttccatc gattcggtca
     7561 tcaccgccgt ggcgatggtc gatcacatcg tcgtggcgat gggcgcggtc gtcgtcgcaa
     7621 tggttgtcat gatttccgcc agcaaactct tgaccgaatt tgtggacagg catcccaccg
     7681 tcgtgatgct ctgccttggt tttttgttga tgatcggttt cagcctgatt gcggaagcct
     7741 tccatttcca cattcccaaa ggctacctct acgccgccat cggcttctcg attttaatcg
     7801 aactgtttaa ccagatttcg caacgcaaca gccgcaaaaa cgactacatc ggcagctcgt
     7861 ggcgcaagcg caccgccgaa aacgttttgg gtatgatggg catacgcgaa agcgtgcttg
     7921 ctgacgcggg cggcgaatcc gtggatgacg cgcattttga agaaaacgaa aaatcgatga
     7981 tacgcagcgt gctgacgctt gccgaacgcc cgattatggg ggtgatgatt ccacgccgcg
     8041 acatcgaacg gctggacatt tcccaaagcc gcgaagaaca gtatgcccaa ctgcaaaaca
     8101 cgccttacag ccgcctgctc gttgtcggaa aggcgggcgt ggacgaaccg ttgggctaca
     8161 tcaacaaaaa agacctgctg tcccaactgc tggaaacagg cggtctcgac attcagacgg
     8221 cattgcgcca gccgctcgtc ctgcccgacg gcacgaccgc cttgggcgcg ctcgaactct
     8281 tccgccaaag cagcgcggat tacgctttgg tggtggacga gttcggcgcg gtgttgggta
     8341 tggtgacgat gaaagacctg ttggaagcca tcgcaggcga gttccccgaa gaattcgagc
     8401 gcgaagaaga acccgccgtt caggggaatc ccgatgaaag cctgacggtg gaaggcgcgt
     8461 tggaatatgt ggagctggca tcccaactca acctgccgca gcaggaggaa gataccgatt
     8521 tccataccgt tgccgggctg attatggaag aattgcaaac catccccgat gtcggcgatt
     8581 ttgccgattt tcacggctgg cggtttgaag tggtcgaaaa agaagggcag cgcatcgagc
     8641 gggtcaaaat caccaaattg cccgaagaat aagcatccag gataaaaaat gaacgttttg
     8701 atttccaacg acgacggcta tctggcagaa ggcattgcca ttctggcgcg ggttgcgtcg
     8761 gaatttgcca atgtcagggt ggtcgcgccc gagcgggaca ggagcggggt cagcaattcg
     8821 ctgacgctcg accgtccttt gcagctgaaa caggcgcaaa acggcttctg ctacgtcaac
     8881 ggcacgccga ccgactgcat ccatgtcggc cagtttgccc tgcccgattt taagcccgat
     8941 gtcgtgtttt caggtatcaa ccgtggcgca aatatgggcg acgatacgct ttattcgggg
     9001 acggttgcgg cggcgaccga agcctatctg atgggtatgc ctgctgtggc gttttcttta
     9061 aacgatgctt ccggacgcta ttgggcaacc gcagaaaagg cactgtggac attgttggcg
     9121 cattttttta aaaaaccgcc gtccgcgccc gtcttgtgga atgtcaacat ccctgctgtc
     9181 gcaccggaag atgtcagggg aatcaagatt acccgtttgg gcagacggca tcatgagcag
     9241 aacatcgtcc cgtcccgcaa tccgcgcggc gaacagattt attggatagg gccggtcggc
     9301 gaagtttccg atcgggaaga ggggacggat tttggtgaat gcggcgcagg ttttattacc
     9361 gtaacgccgc tgcaaatcga tttgaccgcc tatccggaca tggcggagac ggcggccttt
     9421 tggcatacgg actgaccgtt tttcaaatac cgtcgggttt tgcgcttgag aagaacgcgc
     9481 atttttatta tgatataccg cattcaggct atatatcgga acatgaaggt tatcttatgt
     9541 tgaaacaaac gacacttttg gcagcttgta ccgccgttgc cgctctgttg ggcggttgcg
     9601 ccacccaaca gcctgctcct gtcattgcag gcaattcagg tatgcagacc gtatcgtctg
     9661 cgccggttta caatccttat ggcgcaacgc cgtacaatgc cgctcctgcc gccaacgatg
     9721 cgccgtatgt gccgcccgtg caaactgcgc cggtttattc gcctcctgct tatgttccgc
     9781 cgtctgcacc tgccgtttcg ggtacatatg ttccttctta cgcacccgtc gacatcaacg
     9841 cggcgacgca tactattgtg cgtggcgaca cggtgtacaa catttccaaa cgctaccata
     9901 tctctcaaga cgatttccgt gcgtggaacg gcatgaccga caatacgttg agcatcggtc
     9961 agattgttaa agtcaaaccg gcaggatatg ccgcaccgaa aaccgcagcc gtagaaagca
    10021 ggcccgccgt accggctgcc gcgcaaaccc ctgtgaaacc cgccgcgcaa ccgcccgttc
    10081 agtccgcgcc gcaacctgcc gcgcccgctg cggaaaataa agcggttccc gcccccgcgc
    10141 ccgccccgca atctcctgcc gcttcgcctt ccggcacgcg ttcggtcggc ggcattgttt
    10201 ggcagcgtcc gacccaaggt aaagtggttg ccgatttcgg cggcggcaac aagggtgtcg
    10261 atattgccgg caatgccgga caacccgttt tggcggcggc tgacggcaaa gtggtttatg
    10321 ccggttcagg tttgagggaa tacggaaact tggtcatcat ccagcacaat tcctctttcc
    10381 tgaccgcgta cgggcacaac caaaaattgc tggtcggcga aggtcagcag gtcaaacgcg
    10441 gtcagcaggt tgctttgatg ggcaataccg atgcttccag aacgcagctt catttcgagg
    10501 tgcgtcaaaa cggcaaaccg gttaacccga acagctatat cgcgttctga ctttccgttt
    10561 gcaatcaggt taaaaatgcc gtctgaaagg tcttcagacg gcatttttgt tttggcggct
    10621 gtgttttcaa atcattccga agggacgggg cgtataaatt tgtttgactg tgtttgggta
    10681 tttgcttgat ttcatccgtt tgcaatggaa aagccgctta tttccgttcg gagggcggtt
    10741 ttatcggata aaaggcattt tgtccgactg attagggctg catcaaggga atatgaaaac
    10801 acaaccgctt agtctcacgg ctgtgttcca aaatggcgtt gaaaaacgga tgagccagaa
    10861 tcagccgatt ttacttcaat aaatcaaacc attacaccga atgtcggaac aatgcttgtc
    10921 ctgtctttca gacggcatag gccgccgtca gcagccgcag gacacgtctc cggaggcttg
    10981 gaggctgatt tctctgcgtt ttttggcttt ttcgtagcgg cagcgttggc ggtcggtcag
    11041 gatttccagc ggagggacgg ggacgggttt gccgtctttg accgcaacca tcgtgaagta
    11101 gcagctgttg gtatggcgga tttcgccggt gcggatgttt tgtgcttcga cacggatgcc
    11161 gatttccatc gaggtgcgtc ccgtgtagtt gacagcggcg taaaaggtaa ccaaatcgcc
    11221 gatgtggatg ggttctttaa acaggacttt gtcaaccgaa agggtaacgc aataattgcc
    11281 gctgtaacgg ctggcgcagg aataggcaac ctggtcgagc aggagcagga gttcgccgcc
    11341 gtgtacgttg ccgctgaaat tggcggtgtc cggcatcatc agttcggaca taatgagttc
    11401 gtgggaaggc agttggcgtt gttgtgtcat ggtgtacctg ttttaagaaa gggtgttttg
    11461 aaatgggtgc gggaaaattt tagggaatgg ggaagtggaa ttcaagctaa aaaaagctat
    11521 ggagggatag tctgccgaaa tgggccgccg atgccgtctg aagccttcag acggcatttt
    11581 gttcaggaaa gccaaaaacg gaaaaccaag aaaaacacga gtgccagcac gcccgcccaa
    11641 acggcgagca tgaatccggc aaccgcccgt cccatacggt aaaccggttt tttgcgttcg
    11701 tcttcgatgg gggaggcgtg gtgtttttcg ggttgttcgg acattttgtt ctcaatcgga
    11761 aaagttggaa acgggcgtaa ggttacgctg ccgtgcaaaa agatttaaga aggaaggtca
    11821 gcagctgtct tcccaatttt cgtcccaagc gtgtttcaat gccgtctgaa cggtatcggc
    11881 atcaaaaccg cgataggcga ggaaacgcgc ctgtttttgt ttttctttga ggttggcggc
    11941 ggggtgtttg aattttttac gcaacacggc tatggcggct tgcttttcgc ttgagcggtc
    12001 gggaagcagg ttgcggctgg ttttttcatc tatgccctgt tgcgccaaag cctgtttcag
    12061 cctcaatgaa ccgtgtttgc ggcttttgct gcggatatag gcttcggcgt agcgcaaatc
    12121 cgactgccag ttgcgttcgg caaattcgtt taacacgttt tccaactcct cttcgctttc
    12181 ggcgtgcggt gcaagtttgc gtttcagacc gatgcggctg acttcttggc gcgagaggat
    12241 gtccatcgcg cgggcgcgta gggatttttg cggtttcatg gcggcggttt cggcacggaa
    12301 agattcagac ggtatgggaa acggattccg atgccgtctg aacagattgt tttattcttc
    12361 gtctttgttt ttctgcggtc gcaggttttc gtaaatttcg ggcagcgcgc cgataatccg
    12421 gtcggggcgg gtcgtatcgt cttgcgagag cagcgtcata tcgccgtaac cgaaggtaac
    12481 gccgacactc aggcagccgg cggctttggc ggcgatgatg tcgttgcgcg agtcgccgac
    12541 cataagcata ttggcggcat cgatacccaa aacttcggcg gcgtgccgca gcggcagcgg
    12601 gctgggtttt ttttcgggca ggctgtctcc gccgagtatc aggctgaagt agtcggcgag
    12661 tcccagttgt tttagaagct cggcggcaag gatttcgttt ttgttggtaa tgattaccag
    12721 cggaatgccc aaggatttga gcaatgccag cccggcttcg gtttcgggat aggggcgggt
    12781 gaagacgctc aaatggtcgc ggtagtattt catataggac acgaaacctt tttcccacag
    12841 ttcggaatcg gcttcgcggt cgcggtcgtt ggtgaggacg cggtgaacca gtttgccgat
    12901 gccgtcgccg acgtagcttt cgaccacttt ggcgggcagc ggtttcatac cgagttgttc
    12961 caacatcgct tctgcggcgg cggcaaggtc ggggacggaa tcgcacagtg tgccgtccaa
    13021 atcgaaggcg acggcttgga cgtgttcgat ggctgcattc ataatccggt ttcctgtcgg
    13081 gttgaaaggg tttattttaa catcgtttgc acagggcttc taatgtatag attattaaca
    13141 atcgcaacag gtttttcggg cggtgttttc gg
//
LOCUS       DAWTHD010000066        13159 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWTHD010000066 DAWTHD010000000
VERSION     DAWTHD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.066"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="WM704_001438"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="WM704_001438"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFA9340844.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="WM704_001439"
     CDS             complement(734..1447)
                     /locus_tag="WM704_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFA9340845.1"
                     /translation="MKTKLPLFIIWLSVSASCASVSPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="WM704_001440"
     CDS             complement(1447..2115)
                     /locus_tag="WM704_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFA9340846.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="WM704_001441"
     CDS             complement(2126..2311)
                     /locus_tag="WM704_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFA9340847.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="WM704_001442"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="WM704_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFA9340848.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="WM704_001443"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="WM704_001443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFA9340849.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="WM704_001443"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="WM704_001444"
     CDS             6730..7065
                     /locus_tag="WM704_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFA9340850.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="WM704_001445"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="WM704_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFA9340851.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /locus_tag="WM704_001446"
     CDS             8964..9728
                     /locus_tag="WM704_001446"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFA9340852.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="WM704_001447"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="WM704_001447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFA9340853.1"
                     /translation="MMKLNPQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="WM704_001448"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="WM704_001448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFA9340854.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
BASE COUNT         3209 a         3833 c         3419 g         2698 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtgtttttgc gtgttccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtgaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 gatgcgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caactattag gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa atcaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atccccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgagca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccagcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaaca
//
LOCUS       DAWTHD010000067        12933 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWTHD010000067 DAWTHD010000000
VERSION     DAWTHD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.067"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            830..1621
                     /locus_tag="WM704_001449"
     CDS             830..1621
                     /locus_tag="WM704_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9340855.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGASADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            1772..2416
                     /locus_tag="WM704_001450"
     CDS             1772..2416
                     /locus_tag="WM704_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9340856.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            2435..4345
                     /locus_tag="WM704_001451"
     CDS             2435..4345
                     /locus_tag="WM704_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9340857.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEICDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            4367..4733
                     /locus_tag="WM704_001452"
                     /pseudo
     CDS             4367..4733
                     /locus_tag="WM704_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4845..6320
                     /locus_tag="WM704_001453"
     CDS             4845..6320
                     /locus_tag="WM704_001453"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFA9340858.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            6404..7342
                     /locus_tag="WM704_001454"
     CDS             6404..7342
                     /locus_tag="WM704_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9340859.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            7343..8203
                     /locus_tag="WM704_001455"
     CDS             7343..8203
                     /locus_tag="WM704_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme NgoMIV"
                     /protein_id="HFA9340860.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            8220..9356
                     /locus_tag="WM704_001456"
     CDS             8220..9356
                     /locus_tag="WM704_001456"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFA9340861.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            9403..9885
                     /locus_tag="WM704_001457"
     CDS             9403..9885
                     /locus_tag="WM704_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9340862.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            10038..11039
                     /locus_tag="WM704_001458"
     CDS             10038..11039
                     /locus_tag="WM704_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9340863.1"
                     /translation="MQPRSRFKPSVRTKCPGQYRIRRKKHYPAQCLIRCDTVFLQRIQ
                     PLIYQNIPIRTPRHIRQHPTIPRRLQQTGIIRSQRPSSRCRNHEPIFRHRPARKKGLV
                     EKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGANIVIAKTSGNGRYAFL
                     GAYGYGLKANNGNEQEAQKLVASILKNTPVFENGGRAAATTFTQRNIGDVLITFENEA
                     NYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQKTARAYLEYLWSEPAQEL
                     AASLYLRPRNPEVLARHKADFPDLDTFPPEEKFGGWDNIMKTYFADGGVFDRLTAQK"
     gene            11619..12224
                     /locus_tag="WM704_001459"
     CDS             11619..12224
                     /locus_tag="WM704_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFA9340864.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            12287..12649
                     /gene="ssrA"
                     /locus_tag="WM704_001460"
     tmRNA           12287..12649
                     /gene="ssrA"
                     /locus_tag="WM704_001460"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3667 a         3563 c         2880 g         2823 t
ORIGIN      
        1 aatttaccta acgaggtaca gacatgtatt cagaaaacaa gcctaaacgc accgtaaaaa
       61 tgaaaaaata ctggcagcct gcaccctttc cctgtccctt tctgcctgta cttcgactca
      121 agtaacttct tttgtcgagg gactctccga gggactcgta gatgtcagcg aagcctttat
      181 cacaacaaag gcagaatcat attctgcccc gtctcttcag gataagcaaa ttttcataaa
      241 tcgcgtctca gaacgagcac aaaaaaatta caaagaaaga aggaaaaaga aagaagcaga
      301 cttactaaat tcagcatcgt cattaaatga atcaacatca gaagccctat ctgcccttaa
      361 aaacgctatc gtaattccta aacgccctaa acgctccttg ggcaaaaaat ataaaaacgg
      421 ttttcatcct gtaaatacgt gtgcggatgt ggaagctcgg tgggcaaacg gagcaatgag
      481 agatgttagg gacatagaaa ggctaataag ccgaaacgtc cctttacgtc gtatcggaga
      541 cgccattcaa aacatgtgca tggaaaccaa tgtgtccatt aggatgtatc gccaggagtg
      601 ccgttcagaa tcgtcagatt tttatgacga aatgcttgag catcacgaga atttcatagg
      661 atcctccaaa tcattcgcat catcacttct tccccttagc gttacaaact ttacaacaga
      721 catggcgtgt actcccgaat tttaatctaa tcacactcca caactcccta tcaatataac
      781 tgctacttct ctgagtagta tcaaccaacc cttaacaaag gactaaaaaa tgaaaaaagc
      841 actgcttgca ctgactattg ccgccatctc cggtactgct atggcccagt tgcccgactt
      901 tctgggtaaa ggcgaatata ccgtccgtac agacatctcc aaacaaacgc tgaaaaatgc
      961 cgatttgaaa gaaaaacaca aagtacaaaa aaacatcggt ttccgtgccg atatgccgtt
     1021 tgacgatatt caccacggca tgcgtttcga agtatcacac agccgagaca aaaaagacat
     1081 gtacgttgtg accgaaagta ctactaaacc attcggcaaa gacgttaaag aaaaacgcac
     1141 cgacgtgtat gccggttaca cctacaccca accgctcagt gaagccacca aactgcgtgc
     1201 cggtttaggt ttgggctatg aaaaatacaa agatgcggta gctaatgaga aaggaacagt
     1261 cagcaccgag cgcgaagcct tctataccaa agcgcacgcc gacctgactt ccgatttggg
     1321 cggcggctgg tatctgaatc cttgggcaga ggttaaggtt gacctggacg ccaaattgaa
     1381 acacaacgca actgtagccg gtgcctcagc cgacattaac gcaaaaacac gcggatgggg
     1441 cgtgggcgta ggtgcgaata tcggcaaaca aattaccgat accgtcggca tcgaagccgg
     1501 cccgttctac aaacaccgtc acttcaaagc atccggcagc ttcgttttgg acggcgggaa
     1561 catccgagtc gatcctacca aaatcaacga atacggtgtt cgtgtcggcg tgaaattcta
     1621 atcgcggcga tatgccccag acacaaaacc gcaagccttt tggtttgcgg tttttgatgt
     1681 tgtagcagac cctaataccg tctgaaactt ttcagacggc atttttccaa tcggttaaaa
     1741 taccgcctcg acattgtgtt ttggaaaacc cgtgcttgct tctgtgattg attttatcct
     1801 gcatatcgac caacacctgc tcagcctgtc ggcgcaatac ggtgtgtgga tttatgcgat
     1861 tctgtttttg attgtttttt gcgaaaccgg tctgattgtt acgcccttcc tgcccggcga
     1921 ttcgctcttg tttgccgccg gcgggattgc cgcgctgggc ggtatggata ttcatttgat
     1981 ggttgcatta ttaagccttg ccgccatact cggcgacgcg ctcaatttta ccgtcggcaa
     2041 atatttcggc ggcaggctgt tcgccaaccc cgattctaaa atcttccggt gcgaatacct
     2101 cggcaaaacc cgccgttttt acgaaaaaca cggcggcaaa acgattatca tcgcgcgctt
     2161 cgtgcccatc gtccgcacct tcgccccctt cgtcgccggt atgggaaaaa tgcactatgc
     2221 caaatttatc cgctacaaca tcatcggcgg cttgttgtgg gttatcttgt tttcttacgc
     2281 gggctatttc tttgccaact tccccgtcgt aaaaaacaat ctgggcttgg tgatgggcgg
     2341 catcattatt gtttccgtcc tgcccggcat catcgaaatc gcccgcgcca aacttgcggc
     2401 aaaatccgaa cgttaattgt tggaaattga cattatgaat atattatccg tagaaaacgc
     2461 ttcctttgcc gtcggacatg tcgccctgct cgacaaaacg tccttccaac tcgacagcgg
     2521 cgaaaaagtc ggcttgatcg gacgcaacgg cgcaggcaag tcttcgtttt taaaaatcct
     2581 caccggcgtg caaaaactcg acgacgggca gattattgtt caaaacaatc tcaaaatcgt
     2641 ttatgtgccg caggagtcct tttttgataa ggacgcaacc gtatttgata ctgttgccga
     2701 aggtttgggc gaaatttgcg atttattgcg ccgttatcat catgtcagcc atgagttgga
     2761 aaatggttcg agtgagctct tattgaaaga gcttaacgaa ttgcaacttg aaatcgaagc
     2821 gaaggacggt tggaaactgg atgcggcagt caagcagact ttgggggaac tcggtttgcc
     2881 ggaaaacgaa aaaatcggca acctttccgg cggtcagaaa aaacgcgtcg ccttagcgca
     2941 ggcttgggta cagaagcccg atgtattgtt actggacgaa ccgaccaacc atttggatat
     3001 cgacgcgatt atttggttgg aaaacctgct caaagcgttt gaaggcagct tggtcgtgat
     3061 tacccacgac cgccgttttt tggacaatat cgccacgcgc attgtcgaac tcgatcgcgg
     3121 tattttgcgc tcctatcccg gctcgttctc caaatacagc gagaaaaaag cgcaagagtt
     3181 ggcggttgaa gccgaacaca accgcctctt cgacaaattc cacgcgcagg aagaggcgtg
     3241 gatacgcaaa ggcatcgaag cgcgccgtac ccgcaacgaa ggccgcgtgc gccgtttgga
     3301 agaactgcgc cgccagcgtg ccgaacgccg caatgtacaa ggacaggtca acttcaaact
     3361 cgacagcggc aagaaaagcg gcaaaatcat cgccgaactg gaacacgctt cgtttgccta
     3421 tgacgacaaa gttatcatgg ataaattttc cgccatcttg cagcgcggcg acaaaatcgg
     3481 cttaatcggc ccaaacggta tcggcaaaac caccttcctt aagctgattt tgggcgaatt
     3541 gcagccgacc tacggcagaa tccgtatcgg cagcaagcag gaagtcgcct atttcgacca
     3601 gttccgcagc gccttgaatg aaaacgatac tgtgttttac acgctcggac agggcaacga
     3661 ttacgtcgaa gtcggcggca agaaaaagca tgtgatgagc tatttggaag atttcctgtt
     3721 cccccctgcc cgcgcgcaaa gtcctgtttc ttcactctcc ggcggcgaac gcaaccgcct
     3781 tttgctggca aaactcttta ctcgtcccgc caatattttg gttttggacg aaccgaccaa
     3841 cgacttggac atcgacaccc aagaattgct tgaagatctg ttgcgcgatt accaaggcac
     3901 ggtattcctc gtctcgcacg accgtatgtt cctcgacaat gtaattaccc aaagcattgt
     3961 tttcgaagga caaggccgtc tgaaagaata catcggcggc tatcaggact atatcgacgc
     4021 aaaatcgcgg gaagataaaa ttcagacggc atccgcaccc aaagcatccg atgtcgaacc
     4081 cgccaaagaa aaacccaaag ccaaccgtac ggtcaaactt tcctacaaag aacagcgcga
     4141 actcgacgcc ctgcccgacg aaatcgccgc cttggaaacc gaacaggcag aaatcaacgc
     4201 gcagctttcc gatcccggaa ttttcaaaga ttacgaaaaa gcaggagcat tgcaaaaccg
     4261 cgccgaagaa atcgaaatgc tgcttttgga aaagctggaa cgctgggagc tgctggaaac
     4321 caaacagaac ggcaatgccg tctgaaatca aacaggaaga aaaaaaatgt tcaaacgacc
     4381 tgaagaaatc atcgtcctga tacttgccgt cctctggatt gccggtacgt attttctcgc
     4441 cgccctgttt ggagcggatg cttataccgt gttaaaaatc actgccctta ccctgctttg
     4501 gtctgccgca tcattcctcc tttggcaaaa aaaacctcag ccggcatatc tggccgctgc
     4561 tgctcggctg cctgactgcc tgttggtggc cgtatctgaa tcaatcggtc gcacccggtt
     4621 ctttaccttg gcatgcatca tggacgttca aaaccatctt agccctgatt cccgcaaccg
     4681 caggttatct gtttaaatgg aaacaatacc ggcagcgcaa aacaaagccg taagcccgtc
     4741 ttttttcaga cgccatctcc ggtcagactg tcaacaattc cgataaaatg ttatactcgc
     4801 gcccgttgcc gcgccgtccg aaaccgcctt ttgggaaagc agaaatgatc agcaaacaag
     4861 aataccaagc ccaagccgcc caaggctaca accgcatccc gctcgtgcaa gaactccttg
     4921 ccgacttgga tacgccgctt tccctctatc tcaaactcgc caaccgcccc tatacctacc
     4981 tgctcgaatc cgttgtcggc ggcgaacgtt tcggccgcta ttcctttatc ggcctgcctt
     5041 gcagccacta tctcaaagcc ggcggcaaac acgtcgatgt ttatcaaaac ggcgaaatcg
     5101 tcgaacaata cgacggcaat ccgctaccct ttatcgaagc tttccacaac cgtttcaaaa
     5161 cacccgaaat cccaagcctg ccgcgcttta ccggcggctt ggtcggctac ttcggttacg
     5221 aaaccgtcta caatttcgaa cacttcgccc accgcctgaa aaacaccgcc aaagccaacc
     5281 cgctcggcac gcccgatatt ttgctgatgc tgtcgcaaga gttggcggta atcgacaatt
     5341 tgagcggcaa aatccacctc atcgtttatg ctgatccctc gcagcccgac agctacgaac
     5401 gcgcccgcga acgcctcgaa gacatccgca cccagttgcg ccaaagctgc gccatcccac
     5461 tttccctcgg cagcaaacaa acccaagcag tcagcgagtt cggcgaagaa cctttcaaag
     5521 cctgcgtcga taaaatcaaa gactacattt tcgcaggcga ctgtatgcag gtcgtcccca
     5581 gccaacgcat gagcatggaa tttaccgaca atccgcttgc cctctaccgc gccctgcgca
     5641 cgctcaaccc ttcgccttac ctcttttact acgatttcgg cgatttccac atcgtcggtt
     5701 cctcgcccga aatcctcgtc cgccgcgaac gcgacgacgt catcgtccgc cccatcgccg
     5761 gcacgcgcct gcgcggcaaa acccccgccg aagaccttgc caacgaacaa gatttgttaa
     5821 gcgacgccaa agaaatcgcc gaacacgtta tgcttatcga tttggggcgc aacgatgtcg
     5881 gacgcatcag caaaacaggc gaagtcaaag tcaccgacaa aatggtgatt gaaaaatact
     5941 cccatgtgat gcacatcgtt tccaacgtcg aaggctgtct gaaagagggc gttaccaata
     6001 tggacatcct cgccgccacc ttccctgccg gcacactctc cggcgcgcca aaagtccgcg
     6061 cgatggaaat catcgaagaa atcgaaccgg agaaacgcgg catctacggc ggcgccgtcg
     6121 gcgtatgggg cttcaacaac gatatggatt tggcaatcgc catccgcacc gccgtgatta
     6181 aaaacaatac cctcttcatc caaagcggcg cgggcgtggt tgccgactcc gacccaactt
     6241 ccgaatggca ggaaactcaa aacaaagccc gcgcagtggt tcgggcggcg cagatggtgc
     6301 aggaagggtt ggataaataa atttcagaca acctttgcag atgaaaaaaa ggtcgtctga
     6361 aaatcacaaa acaacatcag agatacttac ataaaaaatc acaatgcaat tcacatcatt
     6421 agaaatttgt gccggagcag gcggacaggc tttgggctta gaaagggcag gcttttccca
     6481 tgttgcgtta atcgaaatcg aaccgtcggc ttgtcaaacc ctacgtttaa accgtcctga
     6541 ttggaacgtt attgaaggag atgtccgctt gtttcagggc gaaggatatg acggtattga
     6601 tttattggcc ggcggcgtgc cttgccctcc tttttccaaa gctggaaaac aactgggaaa
     6661 agatgatgaa cgcgatttat tccctgaggc tattcgtcta gctaaggaaa ctgaccctaa
     6721 agccatcatg ttagaaaatg tgcgggggtt acttgaccca aaatttgaaa attatcgcaa
     6781 ccatattaca gaacaatttg ccaagttggg ttaccttgga caatggaaac tactttatgc
     6841 tgccgattac ggagtatcgc aactcagacc cagggtttta tttgttgcgt tgaaaaacga
     6901 atacaccaat tttttcaaat ggcctgaacc aaattcggaa caaccgaaaa ccgttggaga
     6961 actgcttttt gacttaatgt cagaaaataa ctggcagggg gcgcataact ggcgtttgaa
     7021 ggccgcgcag attgcaccga ccttggtcgg cggttcaaaa aaacacggcg gtgctgactt
     7081 ggggcctaca cgatccaaac gcgcatgggc ggagttgggt gtggatggtt caggtttgtg
     7141 ggatagtgcg ccgcctgaag acttttcggg aatgcctaga ttgactgtcc gaatgacggc
     7201 gcgtatccaa ggatttcctg ataactggca attttttggt aaaaaaacgc ctatgtaccg
     7261 ccaaatcggc aacgcttttc ccccgccggt agccgaagcg gtgggcaggc agattatcaa
     7321 agccttaaaa aaggaaaatt gaatgaatcc gctttttacc caagaacgca ggattttcca
     7381 taagaaatta ttggatggca atattcttgc aaccaacaat agaggcgtag tcagcaatgc
     7441 ggatgggagc aatacccgaa gtttcaatat agccaagggg attgccgact tactgcattc
     7501 ggaaacggtt tcggaaagat tacccggtca gacatcggga aatgcttttg aagcaatatg
     7561 cagtgagttt gtccaatctg cttttgagaa attgcagcat atcagacccg gcgactggaa
     7621 tgttaagcag gtcggttctc gcaaccgtct ggaaatcgca cgttatcagc aatacgcaca
     7681 tctgacagcc ttagccaagg ctgccgaaga aaaccccgaa ttggctgccg ccttgggcag
     7741 tgattacacc attactcccg atattatcgt taccagaaat ttgattgcag atgcagaaat
     7801 caaccgtaat gaatttttag ttgatgaaaa tattgccaca tatgccagtt tgcgtgcggg
     7861 taatggcaat atgccgctgc tccacgccag tatctcttgt aaatggacga ttcgcagcga
     7921 cagggctcaa aatgcccgtt ctgaaggatt gaatttggtt agaaacagaa aaggcagact
     7981 gccgcacatt gttgtcgtaa cggcagaacc aacgccaagc cgcatttcat ctattgcctt
     8041 gggtacaggg gaaatagatt gcgtttacca ttttgcctta tatgagctgg aacaaatttt
     8101 acaatcattg aattatgaag acgctttgga tttgttttac atcatggtca atggtaaacg
     8161 attaaaagat atttccgacc ttcccttaga tttggcagtt taaacagaat tattccacta
     8221 tgaacacacc ccccatcctt cctcccgcca tgctcggcat cctcggcggc ggacaattag
     8281 gcagaatgtt tgccgttgcc gctaaaacca tgggctacaa agtaaccgtt ctcgatcccg
     8341 acccgaatgc gccggcggcg gaatttgccg accgccattt gtgcgcgccg tttgacgacc
     8401 gggccgcgtt ggacgaattg gcaaaatgcg cggcggttac gaccgaattt gaaaacgtca
     8461 atgccgacgc gatgcgctct ctggcaaagc ataccaacgt ttcccccagc ggcgactgcg
     8521 tgtccattgc acaaaaccgc attcaggaaa aagcgcggat acgcaaagca ggcttgcaaa
     8581 ccgcgccgta tcaggcggtt tgcaaggccg aagacattac tgaagcaagc gcgcaatttt
     8641 tgcccggcat cctgaaaacg gctacgttgg gctacgacgg caaaggtcaa atccgcgtca
     8701 aaacgttgga cgaactcaaa gccgcgtttg ccgaacacgg cggcgtggat tgcgttttgg
     8761 aaaaaatggt ggacttgcgc ggcgagattt ccgtgatcgt atgccgtctg aacgatgaaa
     8821 acgtgcaaac cttcgacccc gccgaaaaca tccacgaaaa cggcatcttg gcttattcca
     8881 tcgtccccgc gcggctgagt gccgacgtgc agcaacaggc gcggcagacg gcgcaacgct
     8941 tggcggacga attggattat gtcggcgtat tggcggtaga aatgtttgtt gtcggcgaca
     9001 cacatgaatt gctcgtcaat gaaaccgccc cgcgcacgca caattccggc caccatacga
     9061 tagatgcctg cgccgcagac cagttccaac agcaggtacg cattatgtgc aacctgccgc
     9121 ccgccgacac caaattatta tccccttgct gtatggcgaa tattttgggc gacgtttggc
     9181 aggaagatgg cggcgaaccg gattggctgc cgttgcaaag ccggccgaat gcacacctgc
     9241 acctatacgg aaaaaaaacc gcacagaaag gtcggaaaat gggacacttt accgttttga
     9301 ccaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     9361 accaacggca cagatgccgt ctgaagccgc cgacataccg ccatgagcct gctgacactg
     9421 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     9481 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     9541 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     9601 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgcg
     9661 ctttacgtcc accccctgtt ccacaatctc ggactgggca ctgccctgct ccgtcaggca
     9721 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc tctcaactcc
     9781 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     9841 cccagcatca aaatcaaatg cgaactgatg cgcaaacacc tctaatcccg ccttataacc
     9901 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     9961 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcacaggc actctatacc
    10021 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
    10081 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaatgc ctcatacgat
    10141 gtgacacggt atttttacaa agaatacaac cacttattta tcaaaacata ccaatccgaa
    10201 caccccggca catccgtcag catccaacaa tcccacggcg gcttcagcaa acaggcatta
    10261 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcttc cgacatcgac
    10321 ctgctcgaaa aaaaggactg gtagaaaaag gctggcaaca agccctcccc gatcacgccg
    10381 caccctacac cagcactatg gttttccttg tccgaaaaaa caaccccaaa cagatccgcg
    10441 attggaacga ccttgccaaa gacggcgcta acatcgtcat cgccaagacc tcgggcaacg
    10501 gacgctacgc cttcctcggc gcatacggtt acggtctgaa agccaacaac ggcaacgagc
    10561 aggaagccca aaaactcgtc gcatccatcc tcaaaaacac acccgttttt gaaaacggcg
    10621 gacgcgccgc cgccaccacc ttcacacaac gcaacatcgg cgacgtactc atcacttttg
    10681 aaaacgaagc caactacgtc agcaaaaaac tgacccaagg acagtttgaa atcgtctatc
    10741 cgagctacac catttccgcc gaaagccccg ttgccgtcgt caacagcgtc gtcgcgaaaa
    10801 aaggcacgca aaaaaccgcc cgcgcctatc tcgaatacct ttggagcgaa ccggcgcaag
    10861 aactcgccgc ctcactctac ctgcgtcccc gcaaccccga agtattggca cgacacaaag
    10921 ccgacttccc cgacttagac accttccctc ccgaagaaaa attcggcgga tgggacaaca
    10981 tcatgaaaac ctacttcgcc gacggcggcg tattcgaccg cctgaccgca caaaaatgac
    11041 cattatgccg tctgaaccgc ctttcagacg gcatttcaca catcaaaaca gaaaaccgca
    11101 cccaaaatca tcatcacaag acacaacggc aaaccggata caaaacccaa caccgccgcc
    11161 aaaatgccgt ctgaagccgt cggcgcaacc atccttatct ggggcgcgcc tcatcaaagg
    11221 ggcgcataac tttccttaac catccctttt gacagccaaa taaacagtgc ttttgcctgt
    11281 cagaaacaaa accaatattt tgcggttcgt atcggaacat ttataaaaca tcagatttcc
    11341 gtgtttcgcg tttccggcaa ttcaagatta gcaaaaccat taacctgctt cgcaaggcag
    11401 gtttttaaaa ctttatcagg cgttcgagca tatcggttcg accgacccgc atttccggca
    11461 atcaacactg ccgacggttg ccgcatttct ttttgatata caatcacact ttcaaaaaac
    11521 atacgacggc aacaaaatca tcctcccccc gcttggcaaa catccggcaa attaaaaccc
    11581 cgcccgaaac agccatcgga caaataacag gaaaaaatat gaataccgac ttccaaaaga
    11641 tcctcaaaca actgggcaaa caggccaaaa aagaagcggc agaaaaacag gaagccgaaa
    11701 aaaacaagca aaaacaagaa tatgatttgg atttttcaca agcagtcgga caagtctccc
    11761 cattaaaaaa ccggcagcaa tattatgcgc agtccgacaa aacccccatc aaagcccgtc
    11821 ccaaagacaa ccgcaccgac gaagaaaact acttctacat aggcagcaca tacaacgacc
    11881 cacccgccag cttctgcaaa aacggacagg gaaaaaacga catccaacgc ctgaaaaacg
    11941 gatactatcc cgtcgttacc gatgtcgacc tgcacggcta cacccaagaa gaagcacaaa
    12001 aagtcctcaa cgaattcatc gcattcaccc aaaaacgcgg cgtatgcggc gaaatcatcc
    12061 acggcagcgg attaggttcc aaaggctaca aacccgttct gaaaaacatg acccgaaact
    12121 ggctgatgca acaccccgac gtactcgcct acgtcgaacc ccgggaaggc aacgacggct
    12181 gcgtccgcat cctgctcaaa cgcaaattaa ggcagcaaga ctgacaaatt tctccgacag
    12241 acttggcagc accgcacaat atccgtataa tttgcaaacc atttctgggg gcgaccttgg
    12301 tttcgacggg ggttgcgaag cagatgcggg cataccgggg tctcagattc ccgtaaaaca
    12361 ctgaattcaa atagtcgcaa acgacgaaac ttacgcttta gccgcttaag gctagccgtt
    12421 gcagcagtcg gtcaatgggc tgtgtggcga aagccaccgc aacgtcatct tacattgact
    12481 ggtttccagc cgggttactt ggcaggaaat aagacttaag gtaactggtt tccaaaaggc
    12541 ctgttggtcg gcatgatgga aataagattt tcaaatagac acaactaagt atgtagaacg
    12601 ctttgtagag gactttcgga cgggggttcg attccccccg cctccaccat gccctatttt
    12661 cagcagtatc ctgcaacaca aaaaatcctt gaacttcaaa agtttcaagg cttttttatt
    12721 gccttgaata tgatggcgta tgcttgagtt ttcgcaattt atggggtaac ttttggggta
    12781 acttttcagg aatcacccta aaaaatatct taaaaataca aaagccatca cgcatttttt
    12841 aaaaaggtta cccaaaactt aaatgaagcc tttatttata aggcttgcag ctttccaaac
    12901 aagttacccc caaacggata aaaaatgccg aag
//
LOCUS       DAWTHD010000068        12878 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWTHD010000068 DAWTHD010000000
VERSION     DAWTHD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.068"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(92..1219)
                     /locus_tag="WM704_001461"
     CDS             complement(92..1219)
                     /locus_tag="WM704_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9340865.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGVNPFSDIKSTFLIFKALKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEVIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(1573..2798)
                     /locus_tag="WM704_001462"
                     /pseudo
     CDS             complement(1573..2798)
                     /locus_tag="WM704_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3191..4438)
                     /gene="fabF"
                     /locus_tag="WM704_001463"
     CDS             complement(3191..4438)
                     /gene="fabF"
                     /locus_tag="WM704_001463"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFA9340866.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(4594..4830)
                     /gene="acpP"
                     /locus_tag="WM704_001464"
     CDS             complement(4594..4830)
                     /gene="acpP"
                     /locus_tag="WM704_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFA9340867.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5062..6069
                     /locus_tag="WM704_001465"
     CDS             5062..6069
                     /locus_tag="WM704_001465"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFA9340868.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6266..6739
                     /locus_tag="WM704_001466"
     CDS             6266..6739
                     /locus_tag="WM704_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFA9340869.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNILELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFYGPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            6753..7088
                     /locus_tag="WM704_001467"
     CDS             6753..7088
                     /locus_tag="WM704_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340870.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEADNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            7281..9971
                     /gene="glnE"
                     /locus_tag="WM704_001468"
     CDS             7281..9971
                     /gene="glnE"
                     /locus_tag="WM704_001468"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFA9340871.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGIIADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            10395..12602
                     /gene="uvrD"
                     /locus_tag="WM704_001469"
     CDS             10395..12602
                     /gene="uvrD"
                     /locus_tag="WM704_001469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFA9340872.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         3075 a         3562 c         3462 g         2779 t
ORIGIN      
        1 tggattcccg ctttcgcggg aatgacgggg tttcgcggga atggcgggag tttgtcagaa
       61 atcaccgaaa ctcaaaagcc gaccaccttg tttacgcctt caaaatatcg agaaatttca
      121 aatcgacttt ttcggcatcg aatttatctt tggcaatcgc ataacttgca ttccccatca
      181 ggcggacggc agccctgttt tcgataaaat aaatcatttt ttcggccaag atgcggggat
      241 tccaaggttc gatcaggaag ccgttgacct tgtcggcaac cgtttccctg catccgggga
      301 catccgtcgt aatcaccgcc ctgccgacgg ccattgcctc ctgggtgctt cggggaacgc
      361 cttccctata ataagacggc aatacgaata tatgatgttc ttttatcact tcggaaacat
      421 tgtttacaaa accggggaaa cggataatat cgcgggcggc aggctgttcc aaatccccgc
      481 tcccgcgtgg tttgtcgatt gcgcccaaag cggtaaaaac cgtatcgggg tatttgccct
      541 taacctgttc cgccgcccga ataaaatcat caatcccctt ttctttcaga aatctgccga
      601 taaagaggaa ttttacgggt tctttttcat cgggaatatc cgcctcggaa taaggatatt
      661 gccgcaaatc cagaccgatt ccgcccaaaa tatggatgtt ttttattttg atgccgtatt
      721 gatgcagcaa ctcgtctttg tcgtcggggt ttaatacaat caggctttcc aacatcggca
      781 gggtaatgcg gtacaaggcg attaaaatcc cctttatgat ttttgttttt aacggtatgc
      841 cttccggctg cggggtaaat gcgaatccca aaccttccag catcccgacg attctgggca
      901 cgcctgccaa ttttgcggca aaagtgccga aaatcacggg ttttgcgaaa taagggaaaa
      961 ccaaatccgg cgatattttt ttgagtgctt taaatatgag gaaggtggat tttatatcgg
     1021 aaaacgggtt taccccgctg cggtttgacc ggtaggtaac gggtgtaacc cccatttccc
     1081 tgataatgtc caactcattg tcggaaaact ccgatacaaa ggcatacacc tgatggtttt
     1141 tgccgattaa ttttttaatg acgggggcgc ggaaaccgta aatgctggat gcgactgttg
     1201 tgataaaaac gattttcata aggcggacac cttgaatata ggattggaaa tgcggtctgc
     1261 attgatgaaa tgccggatac ggggcgcggc gggcaaacct ttgccaagcg cgccgcatcc
     1321 gacgcttcgc ccgcccgggc aggggcggct tcagacggca ttttatcctg aaatccgctg
     1381 cataacgccc agcgatatac agtatcgcgt tttgtgatga ttttacaccc gaagggaaaa
     1441 cgggcttatt tgaatggcgg tttaatcgcg gcggttattt gcggtttgat aaggctgatt
     1501 tgcgtgccgt gcagtccggc aacgggcggg gtgtttggaa aatgccgtct gaaggtttca
     1561 gacggcattc tgtcatgcgg cggttcgggg ttcgcggacg ggcaggacaa gcagggctgc
     1621 cgtgccggcg agtacggcat cggcgtacca catccagccg tagtcaccga attgtgtaat
     1681 cacgatgccg ccgatgtacg agccgaggaa tccgccgatt tggtgggtga gcatcaccag
     1741 tccgaacagg gtggcgaggt agcgcgtgcc gaagagtttg ccggtaacgg cggcggtcgg
     1801 ggcgacggtg gcgagccatg tgaatccgag tgcggcggcg aaaatgtaaa agttggggtc
     1861 ggttttgggt gagaagatgt aaatcagcac catggcgacg cgtgaggcgt agagtccgaa
     1921 caggatgtgt tttcctttga agcgtccggt gcatcagcct gagaacacgc aaccggcgat
     1981 gtttgccagt ccgatgatgg cgatggatgt cgaggcgacg gtggcgggca gtccgcacag
     2041 ggcgacttcc gtgggtaggt gggttacgag aaaggcgatg tggaagccgc aggcgaaaaa
     2101 gctcaggtgc agcaggatgt agcttggggt tttgaaggcg gttttgactg cttctccgag
     2161 gctttgtccg tgtgtggctt gggtgtgttg ggtgtgggcg gcgttgttgc cgccggcaag
     2221 ccaccatgag acgggcagaa tcagcagggc gattgcgccc caaacgtaaa atgtacccgt
     2281 ccagccgact tcgggcagga cgacgagtcc ttgagtcctt gaaccagcgg tgcgaacagg
     2341 aattgtcctg ccgaaccgcc tgcgttgacc agtccggatg ccaagccgcg tttgtgtgtg
     2401 gggacttggg cggcgacctg tcccataatg atggaaaacc cgccggaacc ggtgccgaat
     2461 gcgagcagca gcccgacggc aatcatcagc ccccaataag tcgggatgtt ggaggcaatc
     2521 aggcaggcgc agacgagcag gagggcaccg ccgcttaata ccctgaacgc gccgaaacgg
     2581 tcggcaagcg cgccggacaa tggttgcaat acgccccaca tcagttggaa aacggcgatg
     2641 atgaggctga actgggcaat gttcaattcg gtggtgttga cgacgggttg gacgaacagt
     2701 ccgagcgtca tcctcatgcc gatggtaatc agcaggatga atgcggcggc ggcaatgacc
     2761 gcccagagtt tgggtgtttt cgatgcggtg tgcgtcataa tatgtatttt gagtcggtta
     2821 agtttgcgaa ttttaacaaa ccggatgatt cgggctaaat aattcctgat tctttggata
     2881 tagccaacgg tatgtctgcc gatgccgtct gaaagggctt gatgggcgtt tgcggttcgg
     2941 gttaatattt gtgccgtatt tgttaattcc agttagtata gtggattaac tttaaaccgg
     3001 tacggcgttg ccccgcctta gctcaaagag aacgattccc taaggtgctg aagcaccggg
     3061 cgaaccggtt ccgtaccatt tgtaccgtct gcggcccgcc gccttgtcct gatttttgtt
     3121 aatccactat aaaaatgccg tctgaaacgg tttcagacgg catttcgatg tcggcggcgg
     3181 ctttgcggaa tcagcctttg aagcgtttga agaccagcgt accgttggtg ccgccgaagc
     3241 cgaaggagtt ggaaatggca acgtcgattt ccgcgtcgcg cgcttcgttg gcgcagtagt
     3301 ccaaatcgca gccggcttca acgtcttgtt caaaaatgtt gatggtcggc gggattttgc
     3361 cgtcgtgtat cgccaaaatg ctgtacacgg cctccacgcc gcccgccgcg ccgagcaggt
     3421 ggccggtcat ggatttggtc gagctgatga cggttttgca ggcgtgttcg ccgaacgcgc
     3481 gtttgagggc tttggtttcg ttggcatcgc ccaagggggt ggacgtgccg tgcgcgttga
     3541 cgtaatccac gtcttcggga ttgatgccgg catctttcag cgcgcgggta acggcaaggg
     3601 cggggccttc ttcgttcggc gcggtgatat ggtaagcatc ggaactcatg ccgaagccga
     3661 cgatttcggc gtagattttc gcgccgcgtt ttttggcgtg ttctaattct tccaacacca
     3721 atatgcccgc gccttcgccg atgacgaagc cgtcgcggcc tttgtcccac ggacgggaag
     3781 cggtggcggg gtcgtcgttg cgggtggaga gggctttcat cgcagcaaaa ccgcccacgc
     3841 ccaaggtgct gattgcgcct tccgcgccgc cggcaaccat tatgtccgcg tcgccgtatt
     3901 tgatcagtcg ggcggaatcg ccgatggcgt gcgcgccggt ggtacaggcg gaaaccatgc
     3961 cgtagctcgg gccgcggtag cctttgagga tggtaacgtg tccggaaatc aggttgatca
     4021 gcgaaccggg gataaagaaa gggttgattt tgcgcgcgcc gccttcgatt acggctttgc
     4081 cggtggcctc gatgctgggc agtccgccga tgccggaacc gatgttcacg ccgatgcggt
     4141 ctttgtcgag gttttccaca tcgtccaaac ccgaatcggc aatcgcctgc aatgcggcgg
     4201 cgatgccgta gtggatgaac acgtccatcc ggcgcgcttc tttcgcgctg atgtattgtc
     4261 cgatgtcgaa accgcgcact tcgccggcga cacggctgtt gatgtcggat gcgtcaaagc
     4321 gggtaatcgc gccgatgccg cttttgccgg cgagcaggtt gtcccaagcc tctgcggcag
     4381 tgttgccgac aggggaaacc tgacctaagc ctgtaatgac tactcttctc tgactcatga
     4441 taacctcgct gttggttgtc ggaatggggg catatgcggc tgtcgtgcag atgccgtctg
     4501 taatttgcgg caggggttca aacagtttgc catataaggg aaaagcctct attgcgcggt
     4561 gcagcagagg ctgttgtgtc gggcgacgac cggttagccg ttgtgggcat tgatgtagtc
     4621 gatagccagt tggacggtgg tgattttttc ggcatcttcg tcggggattt cgcagccgaa
     4681 ggcttcttcc aaagccataa ccagctccac ggtatccaaa gaatccgcgc ccaagtcgtc
     4741 ttggaaggaa gattcgtttt tcacgtcggc ttcgtttacg cccagttgtt cagcaataat
     4801 tttcttaact tgttgttcga tgtttgacat atcagtcgtt cctttatgcc ttgcggcagg
     4861 ttgtttaagg gaaataaatc ggtggtattg taccgatttt taatagagtt ttctatctaa
     4921 tgcctattat atcaatattt gcggatttgt acatttttgg gtgcggcggg ttttgtcgtt
     4981 caagtttgac ctgtgtgccg tatgtttggc gggatttcgg ttaaaatggc ggcatttcca
     5041 tctgaagcag aaagccctgt catgtatcca cttgcccgcc gcatcctgtt tgcactcgat
     5101 gccgaaaaag cccaccactt cacgctcgac gcgctcaaca cggtttataa attgggcctg
     5161 attcccgtaa ccgacaaccg taccaaacct ataaaactga tgggtatgga tttgcccaat
     5221 cctgtcgggc ttgccgcagg tctcgacaaa aacggtgaat acatcgacgc tttgggcgcg
     5281 ctcggtttcg gcttcctcga aatcggcacg gttacgcgca acccgcagcc cggcaacccg
     5341 cagccgcgcc tcttccgcgt tcccgaacac caaggcatca tcaaccgaat gggttttaac
     5401 aaccacggca tcgacgcgat gatacgcaat attgaaaaaa gcaaatatca aggcgtattg
     5461 ggcatcaaca tcggcaaaaa cgcggttacg cccatccaaa acgccgccga tgattattta
     5521 atctgcctcg aaaaagccta cgcgcacgca agctacatta ccgtcaacat ttcctcgccc
     5581 aacaccaaaa acctccgcgc gctgcaaggc ggcggcgaat tgggcgcatt gctcgaagcc
     5641 ctgaaaaaca aacaggcgca gcttgccgcc gcccacggca aatacatccc gctagccgtc
     5701 aaaatcgccc ccgatttgga tgaagcacaa atcgaagaca tcgcccgcgt cgtcaaatcc
     5761 gtcgaaatgg acggcatcat cgctaccaac accaccatcg acaaatcaag cctcggcagc
     5821 catccgctcg caggcgagca gggcggtttg agcggttttc ccgtgcgcga aaaaagcaac
     5881 cgggtgctga aaaaactggc ggaacacata gacggcgcat tgccgattat cggcgtaggc
     5941 ggcattatgg aaggcgggga cgcggcagag aaaatccgct tgggcacgac cgccgtccaa
     6001 gtgtacagcg gattgatata caaaggcccg gcgttggtca aagaatgttt gaaggcattg
     6061 gcgcgatgac gcgccccacc caaaatgccg tctgaacgca cgttttgccg ttcagacggc
     6121 attttcatag attggtatcc gactgttgaa gaggcaatca gccgtcattt ggaagacttt
     6181 tcgtaacagg cgtcaatcga aaggggcgga atgacggggc aggcaaaccg atgcgtgaac
     6241 acggggcagg caaccggagt aaaaaatgaa ccttgattta accgctcaaa aagtccgcct
     6301 ttcttggaag gatattctgt gggggtatga gaataaatac ttgggttggg ctgatgtggc
     6361 agcttatgcc cgaaaaatga cgctttcaga tcatgatgaa cgcgtgttca agctatcttt
     6421 aaccaataaa tccaatattc ttgaattaaa gcctgttcta gaagatttgg cttcggaaac
     6481 gaggggttat tcccctaaaa attggctgta catcctccta aatgacgtat tccatagaaa
     6541 ggaagaattt tacggccctt tgggggaagt tgaaaaaatt tatgcagatt tcgattatcc
     6601 ggaagaaata gaatcatttg tcaggtatat gccgcccaaa gacggctata tcccttctaa
     6661 tcatagttat gaagaaaata ttgcccggtt atattctcac tgggaacact atttgaacaa
     6721 cggcggcggg cagggttaaa accggcaatc cgatgccgtc tgaagcgtta tccggcgttc
     6781 agacggcatt ttgttttccg acagtttata aactgtcgtt gtttcttgac agaaacaacg
     6841 accttattta tttgaaacga ttggaggaca tgattatggg tttttggggt agtgtgggaa
     6901 aagcagcaaa agcagtaggc gagggattga ttgaagccga caatgagcat aaggcattga
     6961 aaatggaata tgcggagaaa tcaagtgagg agctgcatga aatcgtaaag agtgatggtt
     7021 tttttaaaaa ttccacacgg gagaaaggtg cggcttatgc tgttttaaaa gagcgtggcg
     7081 aggtttgaac gggaaacggc ggcatttgcc gctgtttttt atcggcaggc atccgtccga
     7141 atatcggggc aaggtttcag acggcatcga aggttgctat gatatagtgg cttgacttta
     7201 aaccggtacg gctccgcctt gccctgattt gaagttcatc cgctatcaca ttcccgccat
     7261 cctttcaaac ggaatccgaa atgtccgacc accgcctcga caccgcccgc cgccattccc
     7321 tgttcctcgc ccgccagctc gacaacggca aactcaagcc cgaaattttc ctgcccatgc
     7381 tggacaaggc gttgacggat gaggatttcc aagcctttgc cgactgggac aaaatccgcg
     7441 cggaagaaag cgaggaagaa ttggcgcggc agttgcgcga gttgcgccgt tatgtggtgt
     7501 cgcagattat cgtgcgcgat ataaaccgta tcagcgattt gaacgaagtt acccgcacga
     7561 ttacgctgtt tgccgatttt gccgtcaata ccgcgctgga tttcgcctac gcctattatc
     7621 aggacatgta cggcacgccg atcgggcgtt ataccaaatc gccgcagcat ttgagcgtgg
     7681 tggcgatggg caaggcgggc ggctatgagt tgaacgtgtc ttccgacatc gatttgattt
     7741 tcgtctatcc cgaatcgggc gacaccgacg gcaggcgcga acgggggaat caggagtttt
     7801 tcaccaaagt cgggcagaaa ctgattgcat tgttgaacgg cattattgcc gacgggcagg
     7861 tgttccgcgt cgatatgcgg ctgcggccgg acggtgattc gggcgcgttg gtattgagcg
     7921 aaaccgcgct ggagcaatat ctgattacgc aggggcgcga atgggaacgc tatgcgtggt
     7981 gcaaaggccg cgtagtcacg ccgtatccga acgacatcaa atcgctggtg cgccccttcg
     8041 tgttccgcaa atatctggat tacggcgcgt atgaggcgat gcgcaacctg caccgccaaa
     8101 tccgcagcga agtcagcaaa aaaggcatgg cggacaacat caaactcggc gcgggcggca
     8161 tccgcgaggt tgaatttatc gcccagattt tccagatgat acgcggcggc caaatgcgcg
     8221 cgctgcaact gaaaggcacg caggaaacgc tgaagaaaat tgccgagatg ggcattatgc
     8281 cgtctgaaaa cgtcgaaacc ctgcttgccg cctaccgctt cctgcgcgat gtcgaacacc
     8341 gcctgcaata ctgggacgac cggcaaaccc aaaccctgcc gatctcgccc gaacagcggc
     8401 aactgctcgc cgaaagcatg ggtttcgaca gttatgccgc tttttcagac ggcctcaatg
     8461 ttcatcggaa caaagtcaat cagttgttca acgaaatttt gagcgaacct gaagaacaag
     8521 cgcaaagcaa tagcgaatgg caatgggcat ggcaggaaaa acccgacgaa gaagaacggc
     8581 taggccgtct gaaagaacac gggttcgatg ccgaagccgt caccgcaagg ctcgaacaaa
     8641 tctgccacgg acataaatac cgccgccttt ccgcacacgc ccagccgcgc tttgacacca
     8701 ttgtgccgct gttcgtacag gcggcggcag agcaaaacaa cccgacagat acgctgatgc
     8761 ggctgttgga ttttctcgaa aacatcagcc tccgctccgc ctatctcgcc ttcctcaacg
     8821 aacatccgca aaccttggca caactggcgc agattatgag ccaaagctcg tgggtggcgg
     8881 cgtatctgaa caaatatccg attttgttgg acgaactcat cagcgcgcag cttttggata
     8941 ccgcgtttga ttggcaggca ctcgccgccg ccctttcaga cggcatcgaa gcctgcggcg
     9001 gcgataccga agcgcaaatg gacacgctgc gccatttcca acacgcccaa gtcttccgcc
     9061 tcgccgtcca agacctcgcc ggattgtgga cggtagaatc cctctctgac caactctccg
     9121 ccctcgccga caccgtcatt gccgccgccc tttcgtgcgc gtgggcggat atgcccaaaa
     9181 aacaccgcga caccccgcaa ttcgccgtca tcggctacgg caagctcggc ggcaaagaac
     9241 tcggctacgc ctccgacctc gacttagtct atctttacga cgatccccat cccgaagcag
     9301 gcgacgtgta cagccgcctc gcccgccgcc tgaccaaccg gctttccgcc gccaccggcg
     9361 cgggcagcct ctacgaaacc gacctgcgcc tgcgccctaa tggcgacgcc ggtttcctcg
     9421 cgcacagcat cgccgccttt ggaaaatacc agcgtgaaaa cgcatggaca tgggaacacc
     9481 agtccctcac ccgcgcccgc ttcatctgcg gcacacccga aattcagacg gcattcgacc
     9541 gcatccgcac cgaaatgctg actgccgaac gcgaccaaac cgccttggca ggcgaaatca
     9601 tcgaaatgcg cgaaaaaatg ttccccaccc atccgcctgc cgacagcaac gtcaaatacg
     9661 cgcgcggcgg cgtggtcgac gtcgaattta tcgtccaata tctgatactt gcccatgccc
     9721 gccagtatcc gcaactcttg gacaactacg gcaacatcgc cctattgaac atcgccgccg
     9781 actgcggttt gattgacaaa accctcgccg gacaaagccg caccgcctac cgcctctacc
     9841 gccggcagca gcacaacacc aaactgcgcg acgcggcaaa aaccgaagta agcgacgaat
     9901 tgctgtccca ttacggcaat gtcaggaaat tgtggcggga agtgttcggg gaggaagcgg
     9961 caaccgcctg aacaaaaaat gccgtctgaa gcctgacaat ccgggtttca gacggcattt
    10021 gtttttggct gtgtctgaac aatcggtacg atttcggttg tttgcaaatt gaaaggcggt
    10081 tattcctatg ttcaggcggc aaaccgaaat attttcccca accgataggg cgatatcaaa
    10141 gcctattcga ataccgcctt tacggggaag gggctttccg agcttgacca tggtcaggct
    10201 cggcaaatca gcccgcatcc tgtcgatgcg tccgtccaaa tcggtttgga acgccgcaat
    10261 gtgatatccg cctatcaagg ttttgcgtgc gtgtgtcggc gagggctttg ccaaaaccga
    10321 aatcaaaatc aagttgtcag gcatccttcc gtttctatta aaatagcgca ttccattttt
    10381 cagacggcat ccttatgttt cccgaccaat ccgcccccaa tctgctgcaa ggcttgaacc
    10441 ccgaacaact ctccgccgta acctggccgc cgcaatccgc cctcgttttg gcaggcgcag
    10501 gcagcggcaa aacgcgcgtg ctgaccacgc gtatcgcatg gcttttgcaa agcggacaag
    10561 ccagcgtaca cagcattatg gcggtaacgt ttaccaacaa ggccgccaaa gagatgcaaa
    10621 cccgactcgg cgcaatgatt cccatcaacg tccgcgctat gtggctcggc acgttccacg
    10681 gtttgtgcca ccgctttttg cgcctgcacc accgcgacgc aggcctgccg tcttcctttc
    10741 aaatcctcga cagcggcgac cagctttccc tcatcaaacg cctgctcaaa agcctcaaca
    10801 tcgccgaaga aatcatcgcg ccacgttcgc tgcaaggctt tatcaacgcg caaaaagaat
    10861 ccggtttgcg cgcttccgtc ttgggcgcgc ccgatccgca cacaagccgc atgattgggt
    10921 gctacgccga atacgacaaa atctgccaac gcgaaggcgt ggtcgatttc gccgaactca
    10981 tgctccgcag ctacgaaatg ctgcaaagta acgaaatcct gcgccggcac taccaaaacc
    11041 gcttcaacca cattctggtc gacgagtttc aagacaccaa caaactgcaa tacgcctggc
    11101 tcaaactcat ggcgggcggt aacgcagcgg tatttgccgt cggcgacgac gaccaaagca
    11161 tttaccgctt ccgcggcgca aacgtcggca acatgaccgc actgatggaa gaattccaca
    11221 tcgacgcgcc cgtcaaactc gaacaaaact accgctccgt cggcaacatc ctcgccgccg
    11281 ccaacgccgt tatcgaaaac aacgacgaac gtctcggcaa aaacctgcgc accgacgccg
    11341 aagcaggcga caaaatccgc tactactccg cctttaccga cctcgaagaa gcccggttca
    11401 tcgtggatga aaccaaagcc ctcgaacgcg aaggctggga tttggacgaa atcgccgtcc
    11461 tctaccgcag caacgcccaa tcgcgcgtca tcgaacaaag cctgttccgc agcggcatcc
    11521 cctacaaaat ctacggcggc ctgcgctttt acgaacgcca agaaatcaaa cacgccctcg
    11581 cctacctgcg cctcgccgtc aatcccgacg acgacaacgc cctcttgcgt gtcatcaact
    11641 tcccaccgcg cggcatcggc gcgcgcaccg tcgaaaacct tcagacggcc tcaaacgaac
    11701 aaggcatcac cctctggcag gcagcctgca acgccggcgc gaaagccgcc aaagtcgccg
    11761 ccttcgtccg cctgattgaa gccctgcgca accaagtcgg acaaatgcac ctgtccgaaa
    11821 tcatcgtcgg catcctcaaa gacagcggtc tgaccgagca ctaccgaacc caaaaaggcg
    11881 acaaccaaga ccgcctcgac aaccttgacg aactcgtcaa cgccgccatc gagttcaaac
    11941 ccgaagacag caactttgaa accctgcccg aaaacatttc agacgacccc gccttcccca
    12001 tcctcgcctt cttgagcaac gccgccctcg aatccggcga aaaccaagca ggggcaggcg
    12061 aaaaagccgt ccaactgatg accgtccacg ccgccaaagg cttggaattc aacgccgtct
    12121 tcctcaccgg tatggaagag gggcgtttcc ccagcgaaat gagccttgcc gagcgcggcg
    12181 gcctcgaaga agaacgccgc ctcatgtacg tcgccatcac ccgcgcccgc aaacgcctct
    12241 acatcaccat ggcacagcaa cgcatgctgc acggacaaac ccaattcggc atcgcatccc
    12301 gcttcgtcga agaaatcccg ccagaagtat tgcactacct gtccgtcaaa aaacccgcct
    12361 tcgacagcta cggcaacacg cgccaaacca ccgtacaacg ggacaaaatc atcgatgact
    12421 tcaaacagcc gcaaacctac gcaggcttcc gcatcggaca aaacgtccgt cacgccaaat
    12481 tcggcaccgg cgtgattatc gatgccgcag acaaaggcga atccgcccga ctgaccatca
    12541 acttcggcaa acagggcgtg aaagagctgg ataccaagtt tgcgaaattg gaagcgatgt
    12601 aaatttgaaa tgcaggccgg atattcgtat ccgacaaaaa aacatttgac gcgtttatcg
    12661 tttccgaaaa accgctgttg gaaatgtcgg attcgagaat ccgacctacg gcaaaaaacc
    12721 gtagcaagga caaggcaaat agtcgtctga aaacgggaag ggcaattttg ccgcagccgc
    12781 cgccgtcatt cccgcgaacc tacattccgt cattcccacg aaagtgggaa tccggaacgc
    12841 aaaatctaaa gaaaccgttt tatccgataa gtttccgc
//
LOCUS       DAWTHD010000069        12684 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWTHD010000069 DAWTHD010000000
VERSION     DAWTHD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12684
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.069"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..202
                     /locus_tag="WM704_001470"
     CDS             <1..202
                     /locus_tag="WM704_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340873.1"
                     /translation="MTLDQRQELLNYLAEYNNILKFNADGSRVEISTDVQLKHLLYGI
                     DERYYTTALGKEKRLANSVQPI"
     gene            269..1870
                     /locus_tag="WM704_001471"
     CDS             269..1870
                     /locus_tag="WM704_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFA9340874.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            1881..2018
                     /locus_tag="WM704_001472"
     CDS             1881..2018
                     /locus_tag="WM704_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFA9340875.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHR
                     K"
     gene            complement(2181..3518)
                     /gene="ribB"
                     /locus_tag="WM704_001473"
     CDS             complement(2181..3518)
                     /gene="ribB"
                     /locus_tag="WM704_001473"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFA9340876.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(3731..3859)
                     /locus_tag="WM704_001474"
                     /pseudo
     CDS             complement(3731..3859)
                     /locus_tag="WM704_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            complement(4071..4358)
                     /locus_tag="WM704_001475"
                     /pseudo
     CDS             complement(4071..4358)
                     /locus_tag="WM704_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117427.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4559..8831
                     /locus_tag="WM704_001476"
                     /pseudo
     CDS             4559..8831
                     /locus_tag="WM704_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            9346..10039
                     /locus_tag="WM704_001477"
                     /pseudo
     CDS             9346..10039
                     /locus_tag="WM704_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            10466..10699
                     /locus_tag="WM704_001478"
     CDS             10466..10699
                     /locus_tag="WM704_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFA9340877.1"
                     /translation="MGAGGSEAAAPIIGKWLYGKGDGGSLNAEEKETVSAITRMLGTA
                     AGAAEGNSSADAVWGCFQTASDFASSFSYPINM"
     gene            10737..11534
                     /locus_tag="WM704_001479"
     CDS             10737..11534
                     /locus_tag="WM704_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFA9340878.1"
                     /translation="MFLCIAIIARLHYLDQSYETFLFLQKCISSLSLITVSPIIDGCL
                     PTLTSAAPTPEAVSQQQDILQRQREKQLREQMQPEQDVRLDGTDTGIEKMATQVGGAN
                     SDEASPCFPISEVELVGEEAAKFRFALNHALCQTHFVSGKCLHAGDINQIMSLAQNAL
                     IGRGYTTTRILAAPQDLNSGKLQLTLMPGYLRSIRIDRSNDDQTHAGRIAAFQNKFPT
                     RSNDLLNLRDLEQGLENLKCLPTAEADLQIVPVEREPNQSDVVVQWR"
     gene            11565..12593
                     /locus_tag="WM704_001480"
     CDS             11565..12593
                     /locus_tag="WM704_001480"
                     /inference="COORDINATES: protein motif:HMM:NF015803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFA9340879.1"
                     /translation="MDNSGSEATGKYQGNITFSADNPFGLSDMFYVNYGRSIGGTPDE
                     ENFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRYHQAVSGLSEVYDYNGKSYNTD
                     FGFNRLLYRDAKRKTYLSVKLWTRETKSYIDDAELTVQRRKTTGWLAELSHKGYIGRS
                     TADFKLKYKHGTGMKDALRAPEEAFGEGTSRMKIWTASADVNTPFQIGKQLFAYDTSV
                     HAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLPAERGWYWRNDLSWQFKPGHQLYLGA
                     DVGHVSGQSAKWLSGQTLAGTAIGIRGQIKLGGNLHYDIFTGRALKKPEYFQTKKWVT
                     GFQVGYSF"
BASE COUNT         3723 a         2919 c         3114 g         2928 t
ORIGIN      
        1 tatgacgcta gatcaaagac aagaactatt aaattacctt gctgaatata ataatatctt
       61 gaaatttaac gcagatggct ctcgtgtaga aatttctact gatgtacagc tgaaacattt
      121 gttatatggt attgatgaac gttattacac aacagcatta ggaaaagaga aaaggttggc
      181 aaatagtgtt caacctattt aatctgccag caaaccaact aatgctaaac aaaatttaag
      241 cgacgctagc aacaaaccga gacattccat gaccgaacaa cactttaccg aacaaatcaa
      301 atccctaatc gacagcttaa aaaccatctg cgccaactat ggtttgggca atgacggcaa
      361 cgaatttaaa atcatcagtc aggcgttttt gtataaattc ctaaacgaca aatacgattt
      421 tgaagttaaa aaaattcgca aagaaaagcc cgacgagccg atcgagttcg tcaatatgga
      481 tattgatgga aaaacagcgg ttttaaagcc cgaacattcc atcaagtatt tatcagagcg
      541 gcaaaacggc gcggattttg ccaaactctt tgacgatacg ctcaccgata ttgccgcctg
      601 taatgcggat ttgttttcgg tgaaaaccga aggcggggcg aaaatcgtgt tgttcgagcg
      661 tatcagtcag tacattaccg acgaaggccg acgcgacgat ttttgccgcg cgctgatttc
      721 caagctggcg ggtttcagct tcgaggcgat ttttgcgcaa aaattcgact ttttcgccac
      781 gatttttgaa tacctgatta aagactacaa cagtaattcc ggcggcaaat atgccgaata
      841 ctacacgccg cacgcggttg cgcggattat ggcggatatt ttggtgccgg aggaggtgcg
      901 cgggcaaatc cgcagcgtgg acgtttacga cccgtcggcc ggttcgggaa cgctgttgat
      961 gaatgtcgcc catgtcatcg gcgaagataa atgcatgatt tatactcagg atatttcgca
     1021 gaaatcgtcc aatttgttgc ggctgaatct gagtttgaac aatctggtgc attcgctgaa
     1081 taatgtggtt cagggcaata cgattttgtc gcccgcccac aaagacgctt caggctgtct
     1141 gaaaaaattt gatttcattg tttccaatcc gccgtttaaa ttggatttca gcgatttccg
     1201 cgatcggctg gaaagcgatg aaaaccacga acgctttttt gctggtatcc cgaaaatcaa
     1261 gccaacaaag aaagagaaga tggaaatcta tcagcttttt atccagcata ttctgttcag
     1321 cctgaaagaa aacggcaagg cagcgattgt attaccgacc ggctttatca cggcaaaatc
     1381 gggcatcgat aaaaaaattc gcgaatatct ggtggaaaac aaaatgctcg ccggcgtggt
     1441 gtctatgcct tccaatattt tcgctacgac gggaaccaat gtttctatct tgtttattga
     1501 taaagtcaat aaggataaag ttgtattaat cgatgcttcc ggtttaggtg aaaaaattag
     1561 tattaatgac aaccaaaaaa ctgtactttc ccacgaagaa gaacaaaaaa tctgccatac
     1621 cttcacgcac aaacaggcag tagaagattt cagcgtggtg gtcggctatg atgaaatcaa
     1681 agcgaagaat cacagcttgt cggcggggca gtattttgag gtaaaaattg attatgtgga
     1741 tatatccgcc gacgaatttg tgcaaaaaat agcgggattt tcagcggatt tggataaact
     1801 tttcgccgaa tctgccgaat tggaaaagga gattaaggat agattggcga tgttgaaatt
     1861 caattcataa ataccgatga atgaaaccgg tcaaacttaa aatgccgtct gaaagccttt
     1921 cagacggcat tttgtttggt ttggaaaata cttatgccgt tctttccccg aaagagagaa
     1981 tccaaaaaac caaagccaca ggaattcatc ggaaatgaca caaaaacccg acgaaccgga
     2041 ttcccgcctg cgcgggaatg acgaattaga agttacccga aatccgaaaa aagcaaaatc
     2101 caacccaaca gaccgggttt tcgtttgtac ggaaataatg caataaatga agcaaatata
     2161 aagcatttga atttactata ttatttttcc gcttcttcaa agccgacgac ttcgaggccg
     2221 aagccggtga ggccggtgaa agatgacggt tggccgagga cgcgcagttt tttgacgtgg
     2281 aggccggcga ggatttgcgc gccgatgccg tagcttttgc tgtcccattt gtaggcttgg
     2341 tttgcgcctt tgggtagggt gcggtcgagc agggatgcgc cgtcttcggt gtggtgcagg
     2401 aggatgacga cgccgctttc ggcctgttgg acgcgctcaa gggctttggg cagcgaccag
     2461 gaatggcgcg gattggcttg gatgaagtcc atcacgctga agggttcgtg gacgcggacg
     2521 agggtttcgg tgtcggcgga gggtgtgcct ttgacgaggg cgaggtgggt ttcgccggag
     2581 agtttgtcga cgtaaacgtg ttgttggaac tcgccccacg gagtttgtac gggcgcgtcg
     2641 cccatatctt cgagcaggct ttcggtgcgg ctgcggtatt cgatgaggtc ggcaatcgtg
     2701 ccgattttga gcttgtgttc ttcggcgaat ttcatcagtt cgggcatacg cgccatcgtg
     2761 ccgtcgtcgt tgatgatttc gcagatgacg gcggcgggaa tcagcccgtt catttgcgcc
     2821 aagtcgacgc cggcttcggt gtgtccggca cggacgagta cgccgccttt ttgggcgcga
     2881 agcgggaaga tatgaccggg ttggacgatg tctgcgggtt tggcggtcgg ggaaacggcg
     2941 gtttgaatag tcagggcgcg gtcggcggcg gaaatgccgg tggtaatgcc gtgcgcggct
     3001 tcgatggaaa cggtaaagtt ggtaccgtat tgcgcgccgt ttttttgggt catcatcggc
     3061 agaccgagtt tttcgaccat tgcaccttcc ataggcaggc agactaagcc gcgcgcgtgt
     3121 ttgatcataa agttgacggc ttcgggggta acgaattgcg ctgccatcag caggtcgcct
     3181 tcgttttcgc ggtcttcggc atcggtgatg atgaccattt taccggcttt gatgtcggct
     3241 aggatttcgg ggatggggga aatatgggac atggtgtgtt ttcctgtgtg tccgggcggt
     3301 cggccggtgt gttcggtatc gagccagaag gcgggcattt ttgccgcctg ttcgattttg
     3361 cgggcttttt tctcgccgaa ggatttgttt ttcaaaaggg cggagagttc gcctgtgttg
     3421 agggcaacgg cttcggcaaa acggttttgc agtccgccgt agtgttccgc tatccattgc
     3481 cgcaggttgc ggcggcgcaa gccggctgtg tcggtcatcg ttttatcctt tgtagaaatc
     3541 ggatgtagcg gattgtaagc gcgtatttac taaaaagcaa atttgttttt cgccaaatcg
     3601 gattatttgc cttttggtaa acagatgccg tttgaagccg gattcgtggc ttcagacggc
     3661 attgccgcaa ccccgccggc ggggcggctg tttcttcccg cacgcttctg attatggggt
     3721 ttccggaggg ttattccgaa aaaaaattct gataaaaagc cttgattgcc gggtacggca
     3781 taggcggtac gagcaggcgc aataccggca atcctatgat gaatgccgcc ttcccgataa
     3841 aaaccggacg gattgccctt tactttctac aagtaacagg gctttttttt gcccgttttt
     3901 gaggattcgc accatggaag ataaggaagg gatgaaaaag gcggttgccg gcgtgatgac
     3961 ggaggcgccg gcggacggca ggaagccggc aaccgcttcg aatcttcccc ccccttatct
     4021 aacagggggg tacggaaacc gaaacggcgg gcagggttca ggaagtcttc gaatgttacg
     4081 aaacgtacat aacggacggc aaaggaaacc ttaccatttc gtttgagaag cctgaatact
     4141 gcgacttttc cggcagcacc gcgaccgcgt ttacctttgc gttgtccgcc aaaataactt
     4201 tcatctgctt ctacttcgcc atcaaacatt tctaaatgcg gaccgttttg ataaatcagt
     4261 catcgtaaac gatgaaaatc ataggctgcg gtatttttat taacgcctac caactctgcc
     4321 gctgttcttg cagttacgcc tgcgacaaat agcccaatga gtttattttg tttataccgg
     4381 cttagacggc ttttctcatt ggcttattct aacctaaatg ggatttttag ccgttatccg
     4441 gtacgtcccc taatttaata aaaaaatggc ggtaattgac ttaccaaaat ctttgtccta
     4501 gaatgcccag actgtccaat gtttgtaata actctataaa aatcaaataa ctaaaatcat
     4561 gaacaaaacc ctctatcgtg tgattttcaa ccgcaaacgc ggtgctgtgg tagctgttgc
     4621 cgaaaccacc aagcgcgaag gtaaaagctg tgccgatagt ggttcgggca gcgtttatgt
     4681 gaaatccgtt tctttcattc ctactcattc caaagccttt tgtttttctg cattaggctt
     4741 ttctttatgt ttggctttgg gtacggtcaa tattgctttt gctgacggca ttattactga
     4801 taaagctgct cctaaaaccc aacaagccac gattctgcaa acaggtaacg gcataccgca
     4861 agtcaatatt caaaccccta cttcggcagg ggtttctgtt aatcaatatg cccagtttga
     4921 tgtgggtaat cgcggggcga ttttaaacaa cagtcgcagc aacacccaaa cacagctagg
     4981 cggttggatt caaggcaatc cttggttgac aaggggcgaa gcacgtgtgg ttgtaaacca
     5041 aatcaacagc agccatcctt cacaactgaa tggctatatt gaagtgggtg gacgacgtgc
     5101 agaagtcgtt attgccaatc cggcagggat tgcagtcaat ggtggtggtt ttatcaatgc
     5161 ttcccgtgcc actttgacga caggccaacc gcaatatcaa gcaggagact ttagcggctt
     5221 taagataagg caaggcaatg ctgtaatcgc cggacacggt ttggatgccc gtgataccga
     5281 tttcacacgt attcttgtat gccaacaaaa tcaccttgat cagtacggcc gaacaagcag
     5341 gcattcgtaa tcaagggcag ttgtttgctt cttccggtaa tgtggcgatt gatgcaaatg
     5401 gccgtttggt caatagtggc acgatggctg ccgccaatgt gcaagatatg aataatacag
     5461 cggaacacaa agtcaatatc cgcagtcaag cctttgaaaa cagcggtacg gcggtatcgc
     5521 aacaaggcac tcaaattcac agtcaatcga ttcaaaacac tggcaaatta ttgtcggcag
     5581 gaacagagga tttagccgtt tcaggcagcc tgaacaatca aaatggcgaa atagcgacca
     5641 atcaacaact gattattcac gatggtcagc aatctaccgt tgtcattgat aatacgaatg
     5701 gcacgataca atcaggccgt gatgttgcca ttcaggcaaa atcgttatcc aacaacggca
     5761 cacttgccgc tgataataaa ctggatattg cgttacaaga tgatttttat gtagaacgca
     5821 agatcgtggc gggcaatgaa ttgtcgctca gtacacgagg cagcctgaaa aattcacata
     5881 ccttgcaagc aggaaaacgc attcggatta aagcaaataa ccttgataat gcagtacaag
     5941 gcaacattca atccggcggt acgacagaca ttggcacgca gcacaattta accaatagag
     6001 gcttgattga cggacaacaa accaaaatcc aagccgggca aatgaataat atcggtacag
     6061 gtcggattta tggcgacaat atcgctattg cggctacccg cttagacaat caagatgaaa
     6121 acggtacagg tgccgccatt gcggcacgcg aaaacctgaa tttaggcatt gaacaattaa
     6181 ataaccgtga aaacagtctg atttacagcg gtaacgatat ggcggttggc ggcgcattag
     6241 ataccaatga ccaagccaca ggcaaagccc aaaggataca caatgccggc gcaatcattg
     6301 aagctgcagg caaaatgcgt ttaggtgtag aaaagctgca caataccaat gagcatttga
     6361 aaacgcagtt ggtagaaaca gggcgcgagc gtattgttga ttacgaagca tttggacgac
     6421 acgaattatt gcgagaaggc acgcaacatg aattaggctg gtttgtctac aacaatgaat
     6481 cagaccactt acgcacccct gatggagtgg cgcatgaaaa ttggcataaa tacgattatg
     6541 aaaaagtaac gcaagaaact caagtaaccg gaactgcgcc tgctaaaatc attgcaggta
     6601 gcgatttgat tattgatagc aaagcagtct tcaacagcga cagccgaatc atcgccggcg
     6661 gcaatttgct tgtgcaaaca gaaaaagacg gtttgcataa cgagcaaacc tttggcgaga
     6721 agaaagtctt cagcgaaaat ggtaagttgc acaactactg gcgtgcgcgt cgtaaaggac
     6781 atgatgaaac agggcatcgt gaacaaaatt atactttgcc ggaggaaatc acacgcgaca
     6841 tttcactggg ttcatttgcc tatgaatcgc atagcaaagc attaagccgt catgcgccca
     6901 gccaaggcac tgagttgcca caaagtaacc gggataatat ccgtactgcg aaaagcaacg
     6961 gtatttcgct accctatacg cccaattctt ttaccccatt acccggcagc agcttataca
     7021 ttatcaatcc tgccaataaa ggctatcttg ttgaaaccga tccacgcttt gccaactacc
     7081 gtcaatggtt gggtagtgac tatatgctgg gcagcctcaa actagaccca aacaatttac
     7141 ataaacgttt gggtgatggt tattacgagc aacgtttaat caatgaacaa atcgcagagc
     7201 tgacagggca tcgtcgttta gacggttatc aaaacgacga agaacaattt aaagccttaa
     7261 tggataatgg cgcgactgcg gcacgttcga tgaatctcag cgttggcatt gcattaagtg
     7321 ccgagcaagc agcgcaactg accagcgata ttgtttggtt ggtacaaaaa gaagttaaac
     7381 ttcctgatgg cggcacacaa accgtattga tgccacaggt ttatgtacgc gttaaaaatg
     7441 gcggcataga cggtaaaggt gcattgttgt caggcagcaa tacacaaatc aatgtttcag
     7501 gcagcctgaa aaactcaggc acgattgcag ggcgcaatgc gcttattatc aataccgata
     7561 cgctagacaa tatcggtggg cgtattcatg cgcaaaaatc agcggttacg gccacacaag
     7621 acatcaataa tattggcggc attctttctg ccgaacagac attattgctc aatgcgggta
     7681 acaacatcaa caaccaaagc acggccaaga gcagtcaaaa tgcacaaggt agcagcacct
     7741 acctagaccg aatggcaggt atttatatca caggcaaaga aaaaggtgtt ttagcagcgc
     7801 aggcaggcaa agacatcaac atcattgccg gtcaaatcag caatcaatca gatcaagggc
     7861 aaacccggct gcaggcagga cgcgacatta acctggatac ggtacaaacc ggcaaatatc
     7921 aagaaatcca ttttgatgcc gataaccata ccatccgagg ttcaacgaac gaagtcggca
     7981 gcagcattca aacaaaaggc gatgttaccc tattgtcagg gaataatctc aatgccaaag
     8041 ctgccgaagt cggcagcgca aaaggcacac ttgccgtgta tgctaaaaat gacattacta
     8101 tcagctcagg catccatgcc ggccaagttg atgatgcgtc caaacataca ggcagaagcg
     8161 gcggcggtaa taaattagtc attaccgata aagcccaaag tcatcacgaa actgctcaaa
     8221 gcagcacctt tgaaggcaag caagttgtat tgcaggcagg aaacgatgcc aacatccttg
     8281 gcagtaatgt tatttccgat aatggcaccc ggattcaagc aggcaatcat gttcgcattg
     8341 gtacaaccca aactcaaagc caaagcgaaa cctatcatca aacccaaaaa tcaggattga
     8401 tgagtgcagg tatcggcttc actattggca gcaagacaaa cacacaagaa aaccaatccc
     8461 aaagcaacga acatacaggc agtaccgtag gcagcctgaa aggcgatacc accattgttg
     8521 caagcaaaca ctacgaacaa accggcagca acgtttccag ccctgagggc aacaacctta
     8581 tcagcacgca aagtatggat attggcgcag cacaaaacca attaaacagc aaaaccaccc
     8641 aaacctacga acaaaaaggc ttaacggtgg cattcagttc gcccgttacc gatttggcac
     8701 aacaagcgat tgccgtagca cacaaagcag caaacaagtc ggacaaagca aaaacgaccg
     8761 cgttaatgcc atggcggctg ccaatgcagg ttggcaggcc tatcaaacag gcaaaggcgc
     8821 acaaaactta gccaatggta caaccaatgc caaacaagtc agcatctcca taacctacgg
     8881 cgaacagcaa aaccgacaaa ccacccaagt tcaagccaat caagcccaag cgagtcaaat
     8941 tcaagcaggc ggcaaaacta cccttattgc cgaaggtgcg gcgaacaatc caatatcaac
     9001 atcacaggct caggtgtttc aggcagagca ggaaccggcc tgattgccga taagcaaatc
     9061 catctgcaat cagccgagca aagcaatacc gaacgcagcc aaaacaaatc agcaggctgg
     9121 aacgcaggtg ctgccgtatc attcggacaa ggaggctggt cattaggcgt tgccgcaggc
     9181 ggcaatgtcg gcaaaggcta cggctatggc gatagcgtaa cccaccgcca tagccatatt
     9241 ggcgacaaag gcagccaaac ccttatccaa agtggtggcg ataccatcat caaaggcgcg
     9301 caagtacgcg gcaaaggcgt acaagtcaat gccaaaaacc taagcattca aagtgtacaa
     9361 gatagagaaa cttatcaaag caaacaacaa aacgccggtg cacaagttac cgtaggttat
     9421 ggcttcagtg ccagtggcga ttacagccaa agcaaaatcc gagccgacca tgcttcggta
     9481 accgagcaaa gcggtattta tgccggagaa gacggctatc aaatcaaggt cggaaaccat
     9541 acaggcctca aaggcggcat catcaccagc agccaaagcg caaaagacaa gggtaaaaac
     9601 cgattcagca caggcacact cgccggcagt gatattcaaa attacagcca atacgaagga
     9661 aaaagttttg gattgggtgc cagcgttgcc gtaagcggca aaacactggg acagggcgca
     9721 aaaaataaac ctcaagacaa acacctgaca agcatagccg ataaaaacgg cgcaagttca
     9781 tcagtagggt acggcagcga cagcgacagt caaagcagca tcacaaaaag cggcatcaat
     9841 acccaaaaac attcaaatca cagacgaagc cgcacaaatc aggctgacag gcaaaatagc
     9901 ggcacaaacc aaagccgata ttgatacaaa cgtaaccaca gacaccgccg aacgacattc
     9961 gggcagcctg aaaaacatat ttgacaaaga tagagtgcaa agtgaactgg atttacaaag
    10021 aaccgtcagc caagatttta gtaaaaatgt tcaacaaacc aataccgaga ttaaccaaca
    10081 tttagacaaa ctcaaagcag acaaagaagc agccgaaaca gcagcagccg aggcattagc
    10141 caatggcgat atggaaactg ccaaacgcaa agcccatgaa gctcaagatg cggcagcaaa
    10201 agcagataat tggcaacaag gcaaagtcat tctcaacatg ttagcctcag gtttagctga
    10261 gccgacccaa agcggagcgg gcatcgctgc ggctaccgca tcgccagacg tatcgtatgc
    10321 gattggacag cactttaaag atttagccgg tcaaaacgcg aatggcaaac taaccgccag
    10381 tcaagaaacc gctcacgttc ttgcccacgc ggtattagga gcagcggttg ccgcagcatg
    10441 aggcaacaat gccccggcag gagcattggg tgcgggcggg tcggaagcgg ccgccccaat
    10501 catcggcaaa tggctgtacg gcaaaggaga cggcggcagc ctgaatgcgg aggaaaaaga
    10561 gaccgtttcg gcgattacaa ggatgctggg tacggctgcc ggagcagctg agggaaactc
    10621 gtccgccgat gctgtgtggg gttgttttca gacggcttca gatttcgctt cctctttttc
    10681 atatcctata aacatgtgat taaattttca tttatggagt ttttttatct ttttgtttgt
    10741 ttttgtgcat tgctataatt gcccgcttac attacttaga ccaatcctat gaaacctttc
    10801 ttttcctcca aaaatgtatt tcatccctat cccttatcac tgttagtcct atcattgatg
    10861 gctgcctccc caccctaaca tcagccgccc ccacacctga agctgtttca caacaacagg
    10921 atattctcca acgtcaacgt gagaagcaat tgcgtgaaca gatgcagccg gaacaagatg
    10981 ttcgtttgga tggtaccgat acgggcatag agaagatggc aacacaggta ggcggtgcaa
    11041 attcggatga ggcaagcccc tgctttccta tttctgaggt ggaattggtg ggtgaagaag
    11101 cggctaaatt ccggtttgcg ctcaaccatg ccttgtgcca aacacatttt gtttccggca
    11161 agtgtctgca tgcgggcgac attaatcaaa tcatgtcctt agcacaaaat gctttgatcg
    11221 gcaggggata taccacgacc cgtatcttgg ctgcgccaca ggatttgaat agtggcaagc
    11281 ttcaattaac cctgatgccg ggctatctgc gctccatacg aatcgatcgg tccaacgatg
    11341 atcaaaccca tgcaggacgt attgcagcat tccaaaacaa atttcccacc cgctcgaacg
    11401 atctgttgaa tctgcgtgat ttggaacaag gactggaaaa tctcaaatgt ctcccgactg
    11461 cggaagccga tctccaaatc gttcccgtag agagagaacc aaaccaaagt gatgtcgtgg
    11521 tgcaatggcg gtaacgtctg ctgccctact gtgtgagtgt ggggatggat aattcgggta
    11581 gtgaggcgac aggaaaatac caaggaaata tcactttctc tgccgacaat ccttttggac
    11641 tgagtgatat gttctatgta aattatggac gttcaattgg cggtacgccc gatgaggaaa
    11701 attttgacgg ccatcgcaaa gaaggcggat caaacaatta cgccgtacat tattcagccc
    11761 ctttcggtaa atggacatgg gcattcaatc acaatggcta ccgttaccat caggcggttt
    11821 ccggattatc ggaagtctat gactataatg gaaaaagtta caacactgat ttcggcttca
    11881 accgcctgtt gtatcgtgat gccaaacgca aaacctatct cagtgtaaaa ctgtggacga
    11941 gggaaacaaa aagttacatt gatgatgccg aactgactgt acaacggcgt aaaaccacag
    12001 gttggttggc agaactttcc cacaaaggat atatcggtcg cagtacggca gattttaagt
    12061 tgaaatataa acacggcacc ggcatgaaag atgctctgcg cgcgcctgaa gaagcctttg
    12121 gcgaaggcac gtcacgtatg aaaatttgga cggcatcggc tgatgtaaat actccttttc
    12181 aaatcggtaa acagctattt gcctatgaca catccgttca tgcacaatgg aacaaaaccc
    12241 cgctaacatc gcaagacaaa ctggctatcg gcggacacca caccgtacgt ggcttcgacg
    12301 gtgaaatgag tttgcctgcc gagcggggat ggtattggcg caacgatttg agctggcaat
    12361 ttaaaccagg ccatcagctt tatcttgggg ctgatgtagg acatgtttca ggacaatccg
    12421 ccaaatggtt atcgggccaa actctagccg gcacagcaat tgggatacgc gggcagataa
    12481 agcttggcgg caacctgcat tacgatatat ttaccggccg tgcattgaaa aagcccgaat
    12541 attttcagac gaagaaatgg gtaacggggt ttcaggtggg ttattcgttt tgatgcggtt
    12601 gaagtgaata atgtatgaca agatacacaa aagctgccgg ttttttgcag gcagcttttg
    12661 ttttatgttt ggggccgtac cgga
//
LOCUS       DAWTHD010000070        12494 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWTHD010000070 DAWTHD010000000
VERSION     DAWTHD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.070"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            40..1184
                     /locus_tag="WM704_001481"
                     /pseudo
     CDS             40..1184
                     /locus_tag="WM704_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1747..1929
                     /locus_tag="WM704_001482"
     CDS             1747..1929
                     /locus_tag="WM704_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340880.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(1990..2991)
                     /locus_tag="WM704_001483"
     CDS             complement(1990..2991)
                     /locus_tag="WM704_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFA9340881.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(3068..6901)
                     /locus_tag="WM704_001484"
     CDS             complement(3068..6901)
                     /locus_tag="WM704_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HFA9340882.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKGVELFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            7162..7258
                     /gene="ffs"
                     /locus_tag="WM704_001485"
     ncRNA           7162..7258
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WM704_001485"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7481..7900
                     /locus_tag="WM704_001486"
     CDS             7481..7900
                     /locus_tag="WM704_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340883.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            7997..9688
                     /gene="dld"
                     /locus_tag="WM704_001487"
     CDS             7997..9688
                     /gene="dld"
                     /locus_tag="WM704_001487"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFA9340884.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            9873..10244
                     /locus_tag="WM704_001488"
     CDS             9873..10244
                     /locus_tag="WM704_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFA9340885.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            10384..10933
                     /locus_tag="WM704_001489"
                     /pseudo
     CDS             10384..10933
                     /locus_tag="WM704_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            11069..12151
                     /locus_tag="WM704_001490"
     CDS             11069..12151
                     /locus_tag="WM704_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFA9340886.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRVVKEKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVEGGYTVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            12284..12454
                     /locus_tag="WM704_001491"
     CDS             12284..12454
                     /locus_tag="WM704_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFA9340887.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2896 a         3384 c         3423 g         2791 t
ORIGIN      
        1 tgcccgatgt ttttagcaaa tttacaaaag gaagccgata tgcgaaaaaa acttaccgcc
       61 ctcgtattgt ccgcactgcc gtttgcggca gttgccgatg tcagcctgta cggcgaagtc
      121 aaagctggtg tggaaggcag gaacatccgg ctgcagttga ccgagccacc ctcagaaggt
      181 caaacgggca atacagttac taaggccaaa agccgcatca ggacgaaagt cagtgatttc
      241 ggctcgttta tcggctttaa gggggtgggg atttgggcgg cgggctgaag gctgtttggc
      301 agctcgagca agacgtatcc gttgccggcg gcggcgcgac ccgttggggt aacagggaat
      361 cctttatcgg cttggcaggc gaattcggca cggcgctcgc cggtcgcgtt gcgaatccgt
      421 ttgacgatgc cagcaaagcc attgatcctt gggacagcaa taataatgtg gcttcgcaat
      481 tgggtatttt caaacgccac gacggtatgc cggtttccgt gcgttacgat tcccccggat
      541 tttccggttt cagcggcagc attcaatttg ttccgagtca aaacagcaag tccgcctata
      601 cgcctgctac tttcacgctg gaaagtaatc agatgaaacc agttccggct gttgtcggca
      661 agccggggtc ggatgtgtat tatgccggtc tgaattacaa aaatggcggc tttttcggaa
      721 attatgccct taaatatgcg aaacacgcca atgaggggca tgatgctttc tttttgttct
      781 tgctcggcag agcgagtgat accgatccat tgaaaaacca tcaggtacac cgcctgacgg
      841 gcggctatgg ggaaggcggc ttgaatctcg ccttggcggc tcagttggat ttgtctgaaa
      901 atgccgacaa aaccaaaaac agtacgaccg aaattgccgc cactgcttcc taccgcttcg
      961 gtaatacagt cccgcgcatc agctatgccc atggtttcga ctttgtcgaa cgcagtcaga
     1021 aacgcgaaca taccagctat gatcaaatca tcgccggtgt cgattacgat ttttccaagc
     1081 gcacttccgc catcatgtct gccgcttggc tgaaacgaaa taccggcatc ggcaactaca
     1141 ctcaaattaa tgccgcctcc gttggtctgc gccacaaatt ctaaatatcg gggcggtgaa
     1201 gcggatagct ttgtttttga tggcttgcct tcattcttcg attgcaatct gactgccaat
     1261 ctgcttcagc cccaaacaaa aatccggata cggaagaaaa gcggcaataa agatagcaaa
     1321 tcccgtctga agccctgatt gggcttcaga cgggatttcg catacggctt cccttttcag
     1381 attcggatta aacatgctat atttatgaat ctttcaaacc gttttccaaa tgaggtaagg
     1441 aaatgagcga aaccgcgaaa atcaaactca acgaccggcc cgaaaatgca gaaaaaccaa
     1501 acgaaaaagt cgaactgccc attgtcgata acgacaaaaa aggcggacac ggcgaaggcg
     1561 gttgctgcgg ctgatacgtt tttcggcaaa gcagggcatc tgaaaagttt cgcaaaattt
     1621 tcaggtgccc ttttttgttt tcaaggtaaa tgaatgaaat actgcggtca gaagatttat
     1681 ttgctcgtgc gcttgtgata aattcggcac tgtatttgat atgattttaa caaatttgga
     1741 ggggcaatgt tttggtatgt aatcggcttt tgcgcctttg tcgtcgcgct gctgtcgctg
     1801 tgggtcaatg ccggcgcgtt cggtatgcag gaagacgata ccccgcaatc ggattatgaa
     1861 aaacgtttgg gtttgggggc gaaactgaaa aacaaaaata caccgaaagc aggggaaaaa
     1921 cggcaataaa gataacaaat gccgtctgaa aggttttcag acggcatttg ctttgaagcg
     1981 cgggagacat caaacatcca tctcatgctc aaatgcgttc aggattcggc gcacttcatc
     2041 cgcatcttta accgttcgga cgcggtcgaa cagcgtttgc gccgggtcga acgttttttt
     2101 catcatgccc agccattgct taagacgggc gatcgggtat ttgttgtttg cctcttttgt
     2161 caggcacagc tcgaaaaact gccctatcca tgtggaaact tcggcaaaat ccgtgtcttt
     2221 gaccggtccg ccgttctcgt attgcttgat ttggcgcgcc aaatcggggc ggatgaccgc
     2281 accgcgaccg agcatcacgc tgttgcagcc gctgattgtt ttgatgccga tatagtcctg
     2341 caggctgaaa acgtcgccgt tggcggtaac gggaatattg acggtatcgt ggattttcct
     2401 tatccattcc caatgcgccg ggggttcgta gccctcggct ttggtgcgcg cgtgcacggt
     2461 cagtccgcac gcgccccctt cggcaatcgc acaggcgcat tccaaagcag ggcttttgtc
     2521 ttcatagcct agccgcattt tccccgtcag cggaatatgt gcaggcaaac gtccgcgcag
     2581 cgttttgacg atgtggaata tcagttccgg ctctttcaga agaattgcgc cgcctttgtg
     2641 tttgttgacg gtgggcgcgg ggcagccgaa gttcaaatcg attttgtccg cgccgaatcg
     2701 gacggcttcc aatgcgtttg ccgccatatt gtccgcatcg ctgcccagaa gttggacggt
     2761 gcagggcgtg ccggaaaacg ttttgtttcc gttggcgatt tcggggacat attttaacca
     2821 tatggatcgt gaatgcacgg tatgggtaat gcgtacaaat tcgctgacgc attcgtcgta
     2881 gccgccgata cgtgtcagca ggtcgcgcat cacatcgtct accagccccc gcatgggggc
     2941 tggtatgatt ctggtttttt gtttgggttc ggttgtttgt ccgtcaatca tgattcgggt
     3001 aggtatttga ttcaaacaat gccgtctgaa ggctttcggc gttcagacgg catttccgtg
     3061 tccgttgtta tagcaacact ttctctacgc cgccgttgtt ggcttttttt acaaactcat
     3121 ccagccagtt ttcgccgagg atgtgtttcg ccatttcaat gacgatgtag tcggcaggca
     3181 tattgttgtc gtcggaatag cggctcagtc cttgcaggca ggcggggcag gaggtcagca
     3241 ttttgacggg ttcgccctgc ggcagctctt tgaggttttt ctcgatttcc tcttgtttgc
     3301 ggaacttgac ctgtgtggcg atgtcggggc gtttgacggc gaacataccg gattcgccgc
     3361 agcagcggtc gcttaaaacg actttctgcc ccatcaggct gctggccatt tgggtggcgt
     3421 tcatggtttt aatcggggtg tggcaggggt cgtggtagag gtattgctga cctttcacgc
     3481 cgtcgagttt cacgcctttt tcgagcaggt attcgtggat gtcgatgatg cggcagccgg
     3541 ggaagatttc ctcgaagcgg tatttttcaa gctggtcgta acaagtgccg caactgacga
     3601 cgacggtttt gatgtcgagg tagttgaggg tgttcgccat acggtggaag gcgacgcggt
     3661 tgttggtgct catttcttcg gcttttgcct tgttgccgcc agcgtcttgc ggatagccgc
     3721 aacacatata gccgggcggc aggacggttt gtacgccgac gtgccagagc atggcttgga
     3781 cggcgagtcc gatttggctg aacaaacgct ccgaaccgca gccggggaag tagaacacgg
     3841 cttcggcatc ttcgggcgcg gcggggttgt ggatgatggg gatgcttttg ccgtcttcga
     3901 tgcccaataa ggagcgcggt gttttggcgg gtacgctttt gggcagtggg cggttgatga
     3961 aatggataac ctgttcttta atcggggctt tgccgacggt tgccttgggt tcggcttttt
     4021 gctttttcgt gccgatcgga agaagcttgc cgattttgta ggcaaagttc tgcgcgggga
     4081 agccggtctg tatcatcgcg gcgcgcaggg ctttgatggt tttcggaccg gtggcgttca
     4141 aaaacgccat acccattgaa gccgcaggcg caaagcgttt gtggccggaa tcggcaaggt
     4201 agttgcggac ggctacggta acgtcgccga agtcgatgtt gacggggcag ggtttgacgc
     4261 agcggtggca cacggtgcag tggtcgccga tgtccatcag ttcttcaaaa tgtttgacgg
     4321 aaacgccgcg ccgggtttgt tcttcgtata agaaggcctc ggtcagcaaa cccacgccga
     4381 ggattttgtt gcgcgggctg tacagcaggt tggcgcgcgg aacgtgggtg gagcagacgg
     4441 gtttacattt gccgcagcgc aggcagtctt tgacggaatc ggcaatcgtg ccgaggtctg
     4501 atttttccat aatcagcgat tccgcgccca acagctcgaa agacggcgtg taggcgttgc
     4561 gtaagtccga acctttcatc agtttgtggc ggttgaaggt gtgcttgggg tcgacttggt
     4621 ttttgtagtt ccaaaacggt tgcaaatctt catcggtgag gaattcgagc ttggtgatgc
     4681 cgatgccgtg ttcgccggaa atcacgccgc cgagcgaacg ggcgattttc ataatgcgtt
     4741 ccacagaacg gtaggccgtc tgaagcattt cggcatcgtc tgagttgacc ggaatattgg
     4801 tgtggacgtt accgtcgccg gcatgcatat gcagggcgac aaagacgcgg ctgcgtacgg
     4861 ttttggcgtg gattttgccc aagccttgga taattttggt atcggttttg ccgctgaaga
     4921 tttcggcaag cggcttcatt acgtctgctt tgacagacac gcgcaggcgg aaatcgcgga
     4981 aggcggtgaa gcagctttca tcgtctttgg cttcgggcgc ggcgtggacg gctgtaccgt
     5041 agcgtgattt gtagtcggca agcggcgcat ccaaatgggc gagcagccag tcccaacgcg
     5101 ctttgacggc ggcaacgtgg gcgagggcgt gtttgccgcg ttcgcccaac agttcggcgg
     5161 tcggcaggtc ggtgcccatt ttgtcgatgg ggagtttgcc cgaaagatat tgctccaaag
     5221 cggcacagag tttgagtttg ttttggatgg aaagctcgat gttgatgcgt tcgatgccgt
     5281 ccgaatactc gcccagccgc tccagcggaa tcaccacgtc ttcgttgatt ttaaaggcgt
     5341 tggtgtgttt ggcgatggcg gcggtgcggc tgcggtcgag ccaaaaggtt ttgcgcgctt
     5401 cgggcgatac ggcgataaag ccttcgccgt cacgggcgcg ggcaagttcg cagatgtgtt
     5461 cggcggctgc ctctacggcg gcttcgtcgt ctgaaaccac gtccgccagc aagaccattt
     5521 tcggtcgtcc tttgcccgcc gctttggtgg cgtagccgac ggcgcggaca taacgccagt
     5581 ccaaatgctc caaacccgcc agccgcacgc tgtcgtgtgc gagcagaaaa tcgcggattt
     5641 cgacgataga aggcgtggcg gtggcggccg tgccgaaaaa ctccatacac acggtgcgcg
     5701 tgtatttcgg cattttgtgc aatacgaagg caacgccggt aatgatgccg tccgtacctt
     5761 ctttctgcac gccgggcagt ccgctcaaaa atttgtcggt aacgtctttg cccaaaccga
     5821 ctttgcggaa tttgtgtccg gggatttcca agcgttcggt tttaacgatg ttgatgccgt
     5881 ctgaatccag cgtgtgcacg tcgaacacgg cagtttcttc gtcgtggatt ttgccgaaat
     5941 tgtggcgcac gcgttcgata cgcagccatt cgccttgagg gttaaccatg ttccagtagg
     6001 cgaggttgtc caaggcagta ccccacaata cggctttttt accgcccgca ttcatcgcca
     6061 cattaccacc cacgcaggac gcgtcggcgg aagtcggatc gacggcgaac accaagcccg
     6121 cctgatgcgc ggtttcttcc acccgccgcg taaccacgcc cgcgccgcac cggataatcg
     6181 gatgtctgcc gtacaagcct gccagctcaa cgtattcgac gctgcgatgc ttgtcgagtt
     6241 tttcggtatt gatgactgcg ctgtttgcgt ccaaaggtac cgcgccgccg gtataacccg
     6301 taccgccgcc gcgcgggata atgaccaaat ccagctcgat taaggcgcgc accaaaggcg
     6361 cgacttccgc ctccgtgtcg ggattgacga cgacaaacgg atattcgacg cgccagtcgg
     6421 tcgcatcggt aacgtgtgtt acccgcgcca gcccgtcaaa cataatattg tgcggcttgg
     6481 tgattttgct caaacgctcc aaaatctgcc gccgcttttg gcgcgtttcg tcaaagctgc
     6541 cgtcaaaaag ctccactccc ttttccgccg ccgcaatcaa cacatccgct tgctgattat
     6601 cgtcgcggcg tttgcggatt tcgttcaacc tgtggcgcat ttcccgcacc agcgcggcgc
     6661 ggcgtttcgg atgctccagc aaatcatcga ccagatacgg attgcgtacg accacccaaa
     6721 tatcgcccaa cacttcaaac agcatccgtg ccgaacgccc ggttttgcgc tgtccgcgca
     6781 agtcctgtag aatgtgccac gcctcgtcgc ccagcaggcg gatgacgatt tcgcggtcgg
     6841 tgtaggaagt atagttgtag gggatttccc gaatacgctg cggggcggta gtcgtggtca
     6901 tagtgtgtcc gtgtttggca gtttttattt gaatgccgtt tgaaaagtcg gtttcagccg
     6961 ggtttgaaaa ggcgataatg tagttcaatt tttgatattt ttcaatgccg aaacaggcaa
     7021 tacccttata gaaacaaaat aacgctttga gaaattgttg acaatattat caaatataaa
     7081 ttttaggcgg tgtttccggc gtatgaccgt atcaaaacgg ttgagttttt cagaaaaccc
     7141 gttataatgc cgtttctccg aggcgggtct ccccgcatgg caaatcggaa caccgggtca
     7201 ggggcggaag ccagcagccc actccgatgc gccagtgccg ggggtttggt ccgccgccct
     7261 atttgaaacg ccggagcttc gatgttccgg cgttcccgta tcgcaccgat gccgtctgaa
     7321 agccgttcag acggcatttc ctttactttg ccataacgca tcattacaat gtatccatac
     7381 aggccaatga agaaccggct tcataaaaat ggaagtatgg ttcaatttca agttatatga
     7441 cgaacagggc ggtttgatta aagactaaag gagtaggcaa atgaaaaaac ttctaatgat
     7501 aaccctcacc ggtatgcttg cagcttgttc aacaggtgtc aatgtcggcc ggttgatggt
     7561 tgaaatgccg cagggagaac gctctgttgt cgtgcaggtt cccgcgacga ataacccgct
     7621 ttccgatgcg gtggctgtcg gaatgattaa aacatccgga tcgccttcgg catcaaatat
     7681 gattgaaatg ctcggcgcgg acaatatcaa cgtcggcgtg gcgggaggca gccaaatgtt
     7741 taataaggcg accgcacttt attccttaaa ccatgcaaag aaagtcggaa ataatgtcag
     7801 tgtctatatg acgggcgata gcgaaagcga caaggccgat ttggaaaacg cggcaaatgc
     7861 caaaaatatt aaattacatt atttctttaa ccaaaaataa tttgcagcgt gcaacttgcc
     7921 gcaccaaaat atccaaattg aacatacggc ggcgtaccgg gatttgcgcc gccgcttatc
     7981 ccgataggag agtgttatga acgcctcgca attaatcagc agcctgaccc aaaccgtagg
     8041 cgaaaaatac atcatcaccg accccgcgaa aaccgaacaa taccgccaag gctaccgttt
     8101 cggcgaaggc aaggcgttgg cggtggtgcg cccgggaagc attttggaaa tgtggaaaat
     8161 tctgcaggcc tgcgtcgaag cggacgtgat tgtgattacg caggcggcga ataccggact
     8221 gaccggcggc tcgacccccg acggcaacga ttacgaccgc gacatcgtga ttgtgaacac
     8281 catgcggatg aacatcatcc aaaccatcaa caacaacgaa caagtcgtct gcctgcccgg
     8341 ctcgaccctg aaccagcttg aactgctgct gaaacctttg ggacgagaac cgcattcggt
     8401 catcggctct tcttgcatcg gcgcgtccgt tttaggcggc gtgtgcaaca actccggcgg
     8461 cgcgttggta cagcgcggcc cggcctacac cgaaatggcg ttgttcgccc aaatcaacga
     8521 agagggcagg ttggaactgg tcaaccactt gggcatagac ttgggcgaca cgcccgaaga
     8581 aatcctgacc aacctgcaag gtcatcatta tcagaaaaaa gacatcaaac aagacgcggg
     8641 caaaggacat gaccacgcct attgcgaaca cgtccgccaa gtggacgaac cgaccgccgc
     8701 gcgtttcaat gccgaccccg cccgccatta cgaagcctcg ggctgcgcgg gcaagctgat
     8761 ggtttttgcc gtccgtttgg acaccttccc gcaagaaaaa caaaccgccg tgttctacat
     8821 cggcacgaac gacatcaacg agctgaccga catccgccgc gccgccttgg gcgaatttga
     8881 aagcctgccc gtttccggcg aatacatcca ccgccacgct ttcgacattg ccgacgtgta
     8941 cggcaaagac acgttctacg tcatcaaaaa attcggtacg caccaactgc cgaaattatt
     9001 tgacttgaag gcgcgcgtgg acaggttcgg caaaaaagtc agcttcctgc ccaaacattt
     9061 ttccgacaag gcaatgcagt tcgtcagcaa attcctgccc gaccacctgc ccaaatccat
     9121 gcgcgattac cgcgacaaat acgaacacca cctgattctg aaaatgggcg gaaaaggcgt
     9181 ggatgaggcg cgcgcgttct taaaagaata tttttcacac cacggcggcg cgtttttcga
     9241 atgcaacgcc gaagaaaccc aagccgccat gctgcaccgt ttcgccgtcg cctccgccgc
     9301 catccgctac cgttccgtgc acgacgacga agtggaggac ttggtcgcgc tggatatcgc
     9361 cctgcgccgc gacgaccgcg actggtttga aaaactgccg ccggaaatcg acaataaaat
     9421 catccataaa ttgtattacg ggcattttat gtgccacgtt ttccatcagg attacatcat
     9481 caaaaaaggc aacggctgta tggcgttgga acacgaaatg ctgcatctct tagaccaacg
     9541 cggcgcgcaa tatcccgccg aacacaacgt cggccatttg tatgaagcca agcccgcgct
     9601 caaacagttt taccgcaaac tcgacccgac caacagcttc aacccgggta tcggcaaaac
     9661 cagcaaaaag aaaaactggg cggaataagc gcgtccgctt tgaaggcagg caatgccgtc
     9721 tgaaggcaaa acaccgttca gacggcattg tcggatctgt cttccccgac gggcatgttc
     9781 aagccgtatg tctgcacacg ggcgcgcgtc tcacaaataa agtctgatcc taccttccca
     9841 aagggcaggg tttcaaccga aaaggaaaca cgatgaaacc atacaaaatc tacacccatc
     9901 ccgccctgcc gccccaagcc gtcaaacaag gctggtcgtg gccgggtctt ttgttcggca
     9961 cgctgtgggc gtgtttcaaa agaatgtggg gtttggggct ggggatgacg ggcgcgatgt
    10021 tcgtgttggc ggtgtttgcc caactggttt acggcgacac gcccgccaca gactcggcgt
    10081 ttaacgtatt gggcttggcg gtttccgtct ggttcggcgc aaagggcaac agcctttatg
    10141 cacgccacct tttatcgcgc ggctataccg aattgcccga aacagtcgaa gccgccaacc
    10201 cccaagccgc attggcgcaa tatttcggac gcgggggcgg gtaggcgtgt ttcccggcaa
    10261 tgccgtctga aaggcttcag gcggcatttc ttataacttt ttgtttgtgg cacagtcttt
    10321 cctattccgt tccgaaatgc gtaccatgcg cccgtcccca atatttgcga acaaggaaag
    10381 aaaatggcac gtttaaccgt acacaccctc gaaaccgccc cccgaagccg ccaaaccgcg
    10441 cgtagaggcc gtactcaaaa acaacggctt tatccccaac ctcatcggcg tattggcaaa
    10501 cgcccccgaa gccttggcgt tttaccaaga agtcggcaag ctcaacgccg ccaacagcct
    10561 gaccgccggc gaagtcgaag tgatccggat cattgccgca cgcaccaacc aatgcggctt
    10621 ctgcgtggca gggcacacca aactcgcaac cctgaaaaaa ctcctgtccg agcaatccct
    10681 caatgccgcc cgcgctttgg cggcaggtga atttgacgat gccaaactcg gcgcgcttgc
    10741 cgcctttacc caagccgtaa tggcgaaaaa aggcgcagta tccgacgacg aactcaaagc
    10801 atttttcgat gcgggctaca accggcagca ggcagtcgaa gtcgtaatgg gcgtagcctt
    10861 ggcaactttg tgcaactacg ccaacaacct cgcccaaacc gaaatcaacc ccaaattgca
    10921 ggcatacgcc taagcaaaca aaaccgcctg aatgcaggcg gtttttcaaa aacaccccct
    10981 caaacaaaaa caagccgccc aagcggcggg aaaagcagcc gcgcatcagg cgcgcccccg
    11041 attcccccgt aaaccttaag gaacaaagat gaacgcccaa accctgcttg ccaacgttgc
    11101 cgaactcgtc aaaaccaagc tcaaacccct cgtcgacgac atcgaccgca aaggatacta
    11161 ccccgaagca tttatgcgcg aactcggcgc aatcggcgca gtcggtatag aagccgaagg
    11221 cggcaacggc ttaggcttgg caacacgaat cgccgtcttg cgcgaaatcg gcaaagaatg
    11281 cggcgcgacc tccttcagcg cgtggtgtca ggcggcttgc gcgtggtatc tgcaccaaac
    11341 gcccaaccgg gtcgtcaaag aaaaatacct cgccgacatc ctgcaaggca aagtattggc
    11401 gggcaccgga atgtccaata ccgtcaaaca ccttgccggc attgaaaaac acaaccttca
    11461 agccgagtgc gtggagggcg gttatacagt caacggtgcg ctgccgtggg tgtccaacat
    11521 cggcgaagac cacatttggg cgaataccgc ccaaatcggc ggcggctacg tcatgttcat
    11581 cacaggcggg caaagggaag gcgtaagcct gcaaaactgc cccgaatttt gcgccctcga
    11641 aggcacgcgc accttcagcc tgaacttcaa agacgtattt atccccgacg aagacatcat
    11701 cgccgcgccc gaacagtttg ccggctacat ccaaagcatc aaagcaggct ttatcctcct
    11761 gcaaatcggc atcggcgcgg gcgtgataga cggcagcctc ggcatcatcc gcctcgccaa
    11821 catcgtcaat gcagaagtca acagctatct cgacgacggc tacgacagcc tcaaagcaag
    11881 gctggacggc gcgtgggcag aaaccgaacg gctcgccgga ttggcgtggg acaacacgcc
    11941 cgacaacctc gccaccctca agctgcgcga agccgccgcc gtactggccc ttgccgctgc
    12001 gcaatccgcc gccctgcatt ccggcgcgaa aggctacctg atgcggagtc ccgcccaaag
    12061 gcgcgtcggc gaagcgatgt ttgtcgccat cgtaaccccc gcaatcaaac acctctgcaa
    12121 agaaatagca gcaatcgaag ccgcaaaata aacaggcgga aaatgccgtc tgaagcacaa
    12181 accgcccaag gcgggacaga tgcggcggca cgcttcagac ggcatccgaa ccaaaccccg
    12241 gcggcccgct gcaaaaaagc acggtacaaa caaggaaaca caaatggctc aatatatgtg
    12301 cggcccctgc ggctggattt acgatgaaga actcggcgac ccggaacacg gcatcgcccc
    12361 cgggacaaag tttgaagaca tccccgacga ctggaaatgc cccgaatgcg gcgtgggcaa
    12421 agaagatttc tacctgttgg atttcgtgat atagcggcag ccgcacccct tggaaaatcc
    12481 ctcggcgtgt tccg
//
LOCUS       DAWTHD010000071        11681 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWTHD010000071 DAWTHD010000000
VERSION     DAWTHD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.071"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            310..1164
                     /locus_tag="WM704_001492"
     CDS             310..1164
                     /locus_tag="WM704_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340888.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGTPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1254..1712
                     /locus_tag="WM704_001493"
     CDS             1254..1712
                     /locus_tag="WM704_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9340889.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2195..3607)
                     /gene="trkA"
                     /locus_tag="WM704_001494"
     CDS             complement(2195..3607)
                     /gene="trkA"
                     /locus_tag="WM704_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFA9340890.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3705..5228)
                     /locus_tag="WM704_001495"
                     /pseudo
     CDS             complement(3705..5228)
                     /locus_tag="WM704_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5535..6341)
                     /locus_tag="WM704_001496"
     CDS             complement(5535..6341)
                     /locus_tag="WM704_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFA9340891.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6393..6644)
                     /locus_tag="WM704_001497"
     CDS             complement(6393..6644)
                     /locus_tag="WM704_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340892.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6746..7516
                     /locus_tag="WM704_001498"
     CDS             6746..7516
                     /locus_tag="WM704_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340893.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7662..8831
                     /gene="metZ"
                     /locus_tag="WM704_001499"
     CDS             7662..8831
                     /gene="metZ"
                     /locus_tag="WM704_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFA9340894.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8926..9132
                     /locus_tag="WM704_001500"
     CDS             8926..9132
                     /locus_tag="WM704_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFA9340895.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            9528..9752
                     /locus_tag="WM704_001501"
     CDS             9528..9752
                     /locus_tag="WM704_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340896.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            9739..11067
                     /locus_tag="WM704_001502"
     CDS             9739..11067
                     /locus_tag="WM704_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFA9340897.1"
                     /translation="MKAFEDKALAVSADMADALADGGAAATAVGCPPRLIGGEQNKTP
                     NPKGAEKTENQEFQFEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTK
                     VTGKGLVSDTEFVVRYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHY
                     GGQCETMLVELNGTGCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQA
                     MLDHDNGHYDVHNMRPKSECRGTAWRNEDGSGKTFYIGKRENSKFTRVYEKGRQFGDV
                     DSPWVRFEGDIEIPLDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKL
                     FHARNQVGKMVNFLRDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHE
                     EQKAINALNIETLFDDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            11081..11392
                     /locus_tag="WM704_001503"
     CDS             11081..11392
                     /locus_tag="WM704_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340898.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            11406..11609
                     /locus_tag="WM704_001504"
     CDS             11406..11609
                     /locus_tag="WM704_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340899.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT         2888 a         2758 c         3276 g         2759 t
ORIGIN      
        1 aatctggtac agacccgaat gttaagcaga aacgattgtc tcaaaaaaag caactttgga
       61 taaatacaat caagaattgg ctgaactagg tgaaaatgaa aagaaaaaag aagaactaga
      121 aaaaaagtaa aacagcaaga aaacaaaatt cagatcagaa aaaggcacat agaacgtcta
      181 aaagaagaca tagaaaaggt tgcggtactc gaagaagaaa tcaaaagatc tgaaaagttc
      241 ttcgatgaaa acggttggct taaagacagc attatcgacg acaaaacaaa aaacgccgtc
      301 aacctcaaaa tgagcaacgg cgcgcgttgg acggttacca atgattcaat gctgaaagag
      361 ttggatttgt cggaagacgc gcaggtcgaa tttagcgaca acaataaatt cgtcaaagtg
      421 tccgtgagca agctcaaggg cgatggcggc gtgttcaaaa tgtatggcga catcgtcaaa
      481 ggagaatcgg acaagctgat tacccgaaaa ggcagcgaag ggacgcacat catcgaatat
      541 atggacgatg ccaaggcaaa aacgacggga agggaatatc tgaaactggt cgaaaacaag
      601 ggcaatcaag aagacaataa agcatcaaat aaagcatcat acaaattgaa tgtccgctgt
      661 acggaacagg gagggtggtg ttttgccttg ggtgagtcgg gtgcttccaa aaaggtcaat
      721 atttccacag acggtaagcg ggacttctac ctctacccgg ataccctgac gccgggcgca
      781 tcgagcagcg tccttttcgg cgaggcgttg tatcagttga acgcggtttc cgatgaaacg
      841 ctggtgcagc gtatgggcga aatccacgcc gacggaacgc cgcaggaaga caacaacgtt
      901 tggatcaaac gcgtcggcgg aaaattctcc ggcagccgca gcgattaccg cgtgggcggt
      961 tacggcaacc gctattgggg ctttgccggc ggcttcaaca ggacggggtt cggcgataaa
     1021 tggattcatt acaaagggct gatgctccgc cacctccaat cgtcctacgc ttccgaagac
     1081 tacgtcggca gcggcaaaat cttacggcag ggcggcaggt gtttattccg cctggctcaa
     1141 ccgggaaagc agggcttatt atgatttggt cgccaacatc gcccgatata agggcagcta
     1201 cgggctgacc aattacgccg gcaaacgggt cgaatcggac gaggcgcgcc tgaatgccta
     1261 tatgctttcc gccgaagccg gtaggcggat ggaaaaacaa gacggcggca aaaacctatc
     1321 ggtggcagcc ggaagtgcag ttgtcttact ggtttacgcg cggctacggc tttccgctgt
     1381 caaacgggct gtctgccgag acggacaact tccgcagcct gatggggcgg ttcggctttc
     1441 gggcgggcgt ggacggtttg gacggcggca ggctgaacat ctacggcaag ctgatgtaca
     1501 aacgcgagtt tatcggcacg atccgccaca ggttcaacgg ctctgccgtg gaggaattca
     1561 aacaccgggg cggctggttg gaatacggct tgggcgtggt gcgccgcaat gccggaaacg
     1621 gacggcagct ttattttgag gcgcaaaggt cttcgatgca cacgatgcgc cagaattggc
     1681 aggtcaatat gggcgtgcgc agtatgttct gatgcgcgga tacgcctgaa aacaatccgg
     1741 aacggtcaga cgcgttccgg attgtttttt tgtcggggcg gtttgctgcc atatcaaaac
     1801 aggcgggcga tgccgtctga aacttcgtca ttcccgcgaa agcgggaatc tagaacctca
     1861 aactttcaga taatctttga atattgctgt tgctcaatgg tttggattcc cgcaaccttt
     1921 cgtcattccc gtgaaaacgg gaatctagaa tctctaaagc ttcagctaac ctttgaatat
     1981 tgccgctttc caatggtcta gattcccgcc tgcacgggaa tgacgatttg gaaattaccc
     2041 gaaacccaaa aacaactgaa accgaacagg ccggattccc gctacgcggg aatgacgggg
     2101 ggcggatgcc gtctgaagcg gtgggctgaa gcccactcta ctattatttt tgcttccata
     2161 ccgcttggcg gcaatgtgtc cggaatggag cggtttatcc gaaaaagccc attttgactt
     2221 ggatgagttt ctccagttcg ttcaggatgc gccggcgcga gacgaaaaag atgatgtggt
     2281 caccgtcttg gatgacggtt tcggtatggt gtcccataat ggtttcgccg gttccggcgc
     2341 ggacgacggc ggcaatgtgg cagccttcgg gccatttgat gccgctgatg cgcctgccga
     2401 tgatggcgga agtttttttg tcgccgtgcg cgacgacttc gatggcttcc gccgtgccgc
     2461 gccggatggg gtggacggca acgatgtcgc cgcgccggat gtgggcgagt atcgagccga
     2521 tggtgatgag gtggggggag acgacgatgt cgattttgtt gccttcgagc aaatcgacgt
     2581 agcttgagcg gttgacgatg ccgatgacgc gcttcgcgcc gaggtttttc gccaaaaggg
     2641 cggacataat gttgctttcg tcgtcgttgg tcagggcgca gaatacgtcg atttcgtcga
     2701 tgtattcgtt gtcgagcagg gtttcgtcgg ttgccgaacc ttgcaggacg agggtgttgt
     2761 cgaggttttc ggctatccat tcggcacggc gcggccggca ttcgatgatt ttgacgttgt
     2821 atgcgtgttc gagctgcttg gcgaggcggt agccgatgtt gccgccgccg gcaatcatga
     2881 tgcggcgggt gctggtttct ttggggcgca attcgggtat gaccgccccg atgttttcgg
     2941 cggcggcggc aaacaggatt tcgtcgcctt cgatgatgac ggtttgcggc gcggggacga
     3001 tgaggcggtt gttgcggtaa acggcgcaga tttggcagtc ggccccgtcg ggcaaatctt
     3061 gggcgatgtc ggcaatgctg cgtccgacaa gcagcccgcc gcgccgcgcc tgtatgatga
     3121 ccatccgcac gcggtcgtct gcaaaacgta aaacctgcaa tgcgcccggg cagtctatca
     3181 ggccggcaag ctgttcggta acgagctgtt cggggctgat ggtttcggtt atgccgaata
     3241 tggaaaggct gccgttttcg ttgttttcga gcttggggct gaggtattcg aggtattcgc
     3301 tggaacggac gcgcgcgatg cggccgggga tgttgaacag gtcggcggca actttgcagg
     3361 cgacgatgtt ggtttcgtcg ctgcgggaga gcgcgagcag caagtcggca tcttccgcgc
     3421 cggcgcgttc taatgtgaag ggggatgcgc cgttgccgaa aacggtttgg acatcgaggc
     3481 ggctgcctgt ttcctgcaat gctttttcgt cgatgtcgat aacggttacg tcgttgttgg
     3541 tgatggcggc aaggttttgc gcgacggtag aacctacctg cccgttgcct aaaatgagga
     3601 ttttcacggt atgggtcgcc gggtgatgaa atggtggatt gtaccgcaac cggcggcgga
     3661 aatgccgtct gaaggcagat tttgcgttca gacggcattg cgcttcaaga ctcgacgggg
     3721 atgatgccga ttttcgcctg ccatttgcgc ggggcggtgg cgtggatgga ttcgcctttg
     3781 ctgtccacgg cgacggttac gggcatatct ttgacttcaa attcgtaaac ggcttccata
     3841 cccaattcgg ggaacgccaa gactttggaa gatttgatgg cttttgccac gaggtatgcc
     3901 gcgccgccga ctgccatgag gtacacggcc ttgttgtcgg cgatggcttc gcaggtggcc
     3961 gcgccgcgct cggatttgcc gatcatgccc aagaggccgg tttgtttgag catttggcgg
     4021 gtaaatttgt ccatgcgggt ggctgtggtc ggacctgcgg gaccgacgac ttcatcgccg
     4081 accggatcga cggggccgac gtagtaaatc aggcggttgg tgaaatcgac gggcaactcc
     4141 tcgcctttgt cgagcatatt gacgaggcgt ttgtgcgcgg catcgcggcc ggtgaggatt
     4201 ttgccgttca acagcaatac gtcgccggtt ttccagcttg ccacttcttc tttggtcagc
     4261 ttatcgacat cgacgcgttt gccgttgtcg gggctgtaag tcagatcggg tcagtcttcg
     4321 acgcgcggcg gcgtgagttc gacaggacct gagccgtcca attcaaattc gacgtggcgg
     4381 gtggcggcac agttgggaat catggcaatc ggtttggagg cggcatgggt cgggtaatcg
     4441 aggattttca cgtccaacac ggtggtcaga ccgcccaagc cttgcgcgcc gatgcccagc
     4501 gcgttgacct tttcaaagag ttcgaggcgc agggcttcgg tggtggacaa ttccgcgccg
     4561 gacgcggctt tttcctgcaa ttcttggatg tcgatgtggc tcatcaggga ttctttcgcc
     4621 atcaacacgg ctttttcggg cgtgccgccg atgccgatgc ccaagatgcc gggaggacac
     4681 cagcccgcgc ccatcgtcgg gatggttttc aatacccaat cgacgatgtt gtcggaaggg
     4741 ttgagcatag cgagtttgga tttgttttca gagccgccgc cttttgccgc gcaggttact
     4801 tcgactttac cgcccggcac gatgctcatg tggatgacgg cgggggtgtt gtctttggtg
     4861 ttttggcgtt tgccggccgg atcggcgagg acggaagcgc gcagggtgtt gccttcccaa
     4921 gtgtaggcgc ggcgtacgcc ttcgttaacc atctcttcca cgctcatgtc cgcatcccat
     4981 tgcacatcca taccgacttt gaggaagacg gttgcgatac ctgtgtcttg gcagatgggg
     5041 cggttgtttt cggcacacat acggctgttg accaaaatct gcgtcatcgc gtctttggcg
     5101 gcgggatttt cttccttctg ccacgcctta taaagcgcgt cgatgtagtc ttttggatgg
     5161 tagtagctga tgaattggaa ggcatcgcag atactttgaa taaagtcttc ttgcttgata
     5221 acggtcatga ttgtgttcct tttcaaggtt gtggcggtaa gtgtctgctg ctgttttaaa
     5281 caaccttttt gaaggctgtt ttttatcagg ttgaaatagt gccagcgttt gtacggtgaa
     5341 gacaatacat aattctaatc tcttgattat caatttgttt taataaaata ctgttttatt
     5401 cggattttga tatctgtaat ctttgcctgt tgctttgcgg tagggagaaa tcagcaggca
     5461 gttcattgtg gaccggttga tgaacaatgc cgtctgaaag ggcttcagac ggcattttgt
     5521 tcggacgggc gggcttattt cgcagcctgt ccgccctctt tggcaaaata tttggcgtag
     5581 atcttgtcgt attcgccgct ttcgcgtact ttttccaacg catcgttcag cattttgacg
     5641 gttgcttcgt cgcctttgcg taccgcgatg ccgtagtgtt cggtggtgaa gtcgggcagg
     5701 gtaacgaagt ccattccttt ggccgggttg tttttcacat aattggcgat gaccgcgctg
     5761 tcgctgacca cggaatccaa gccgccgttt tccagttctt tgataatcag ggggacgttt
     5821 tcgaagcgcg cgattttcgg attgtcgttg cccaagagtt tggaaacgga gaaatcgccc
     5881 gtgtggccgg taaccacgcc gactttgttc atctttttca aatcttcgga agaagatact
     5941 tttttgcctt tcggaacgag gacgacttgg gtgatttcaa aatacgggtc gctgaaatcc
     6001 atagactgtt tgcggtcgtc ggtaatggtt acgcccgaca tcacaacgtc cgcatcgccg
     6061 ttgttcaagg cggggaaaag gctgtcccac ggctggtgtt tgaattcgat tttaaaattg
     6121 cccgccttcg ccatcgcgtt catcaaatcc acgtcgaaac cttcgacatt gcctttcgag
     6181 tctaaagatt caaagggggc aaactcggcg ttggaagcca cgcggtacac tttgccgggg
     6241 ttggcggcag gcgcggcggc atctttgccc tgaccgccgc aggcagacag cgcgagtgcg
     6301 gaacaggcaa gggcggcggc aatccatttt ttcatattca tcaaaattcc tttcggtaat
     6361 caaaaagcct aaaaaatcag tttaacataa aattaacgtt ccttttcacg gaaaaacgca
     6421 taagtaacgt cgtggaacaa atccgtatgc gggctgtcct catagctcaa ttctgccaag
     6481 gcgtcctcaa aagcaagctg aatcgactcg accgcgattt cggcggtgtc tgcgggaagg
     6541 gcgcggatca gggctttgaa ggcggtggac aggacgtggt tctgcatacg cagcacatcg
     6601 tttgcttctt ccagatattc caaacgctgt tcgatgctgc ccatgatgta tcctcgcgaa
     6661 tctgttaaaa tgcgccatta tatcaaactg cgctttgggc gcgggtttgc ccgcctgcaa
     6721 agattaagaa ccggaagccg aaaaaatgga tacaaacaat caggataaag agccgaattg
     6781 ggaaaaacgc ctggaaaacc tgcgtcagac gcgccgtatg aataccgcgc ctgcctatgt
     6841 gccgctgacc aaaccttctg aagtcggaaa ggaagagccc ataccgaaat tggacgatgc
     6901 aaaccgagac cgtgtgttaa aggcgtatct gaagaaatgg caggaagaac acaacagcgc
     6961 cgttgccgat tgcggcgagg aagtcgaaac cgacccgatg atcgtccttc aggaaaactg
     7021 gctcaacgcg caggcttccc tgcaaatgcc ggtttcggaa aaacacatcg aatcgcggcg
     7081 gagaatccgg ttcgatgcca aaaaagacag tgccgaattt gaaaccgcac agattgaggg
     7141 cggggaaaac gccgatgcgg cagaatccgg tacggcggca gacggggttg cggaaaacgg
     7201 cgaaggcggt gcggaagagg cgttgatgcc cgtccaaatc aatatcctga acccgaaagc
     7261 cgtcaaccgg cgcgaagtgt tctgcctgtc cgaacaggag ctgaccgaac ggttgatcaa
     7321 acgcctgcgc ccgcatctga ccgataccgt caacggcatg atccgtacgg cggtgcagaa
     7381 gcagatggcg ctctttactt atcagctcca gcaaatgctg cacgagcagg cgggtgcggt
     7441 agtcgaagac gtgttggaac acgatgtccg taaaatcctc aacgacatca agtacgaatt
     7501 aaaatacaaa cgctgattgc catgccgtct gaaagccgtg ttcagacggc attttcctgc
     7561 ccgtccgact tgaaagcctc atggaacagg cgcaaaatat taataaatta aacaacgggt
     7621 gcacgccccg ttgccgaacg gacaaaaccg aggaatcacc gatgagcaaa aaacttcatc
     7681 cgcaaacgct cgccatacgc ggcggcaaag aacaaaccgg ataccgcgaa cacaatcagg
     7741 cattgttcct gaccagcagc ttcatgtggg acaacgccca acacgctgcc gatttgtttt
     7801 caaaaaaaat caaaggcttc acttataccc gtaccgccaa cccgaccatc gcggcctttg
     7861 aaaaacgcat agccgctttg gaaggcgcgg aacgcgcggt cgccacttcg accggcatgt
     7921 ccgcgattca ggcggcgttt ttcaccttcc tgcaggcggg cggccatgtg gtttccagcc
     7981 gcagcctgtt cggcacgacc gtcggcttta tcaataacat cgttaccaaa ttcggcatcg
     8041 gagtgagccg cgtgtcgccg actgatataa atgaatggaa agccgccgtc aaagccaaca
     8101 ccaaactgct gtttttggaa acgccgtcca accccttggg cgaagtggcc gacttggaag
     8161 ccttggcgga attggcgcac ggcatcggcg cgctcttggt ggtggacaac agcctgctgt
     8221 cgcccgtcgg ctcgcagcct ttgaaacacg gcgcggatat ttccgtttcc tccgccacca
     8281 aagccataga cggacacggg cgcgtgatgg gcggcgtgtt ggcgggttcg gaagaactga
     8341 tggcgcaggt cgccgtttac tgcaactctt gcggtttggc gatgtcgccg tttaacgcgt
     8401 ggcagttgtt gagcggcgtg gaaaccctgt cgctgcgtat ggaaaaacag tttgacaacg
     8461 ccttgagaat cgcgcaatgg ctgcaagagc agccgcaggt tcaagcagtg tactacaccg
     8521 gattgcccga ccatccgcag gcggagctta tccgcaaaca gcaaaacggc ggcggcatcg
     8581 tcatcggttt tgaagtcgcc gaccaagcag ccgcgtggaa agtcgtggac ggcgtggaac
     8641 tcttttcccg taccgccaac ctcggcgacg tgcgctcgac catcaccaat ccgtggacaa
     8701 ccacgcacgg caggatgcag cccgaagaaa aactcgccgc cggtatccgc cccggactgg
     8761 tgcgcctgtc ggtcggtttg gaatatgttg gcgatttgat tgacgatttg aaacaggcac
     8821 tcgcgcgctg attgcgtgtt tcagacggca tcggcggaaa aatgcggcag atgccgtctg
     8881 aaacccttat tgcgtatggc atacacatca acaataggaa aaaatatgga tgaagcggtt
     8941 tttgccgatt gggcgttgaa aatctgcctg accggtttga ttattttttt gggttttatc
     9001 gtttggaatt tgggcaagga gtccaaagca ggcaaattcg gcatcgccgt tttgtttttg
     9061 gttttgggct tgggtgtgtt cggttttgtg ttcaaagagt tattgattaa gtttttggta
     9121 ttgcccaaat aggtttgccg tctgtcgaaa tgccgtctga aatcataaaa agtaatctgc
     9181 accctaaaag tttgatttaa ccgccaactg attaaggtgc gggtttttat gacaaaataa
     9241 aaatcatctt tccgcaatca ttcccacgga tgcgaaagcc tgtctttttg aatcccggat
     9301 aattttacat cgtcattccc gcgcaggcgg gaatctagtc cgttcggttt cagtcattta
     9361 cgataaattc ctgttgcctt tcatttccgg attcccactt tcgcgggaat gacggaggag
     9421 cggataaatt ctcgcaatct aaaaccttat atattgcgct ccataagggg cgcatatttt
     9481 taatccttgt aaagcccctt aactctagtc agtgaagggg cttaatcatg tttattttct
     9541 atactgtcaa tcccgaacac gtatatttcc ccaaagcata catcatgaaa gtgttcaagg
     9601 ataaaggcta cgaatcccaa tgcatcacaa cagtttcttt ttatatctgc aatccgactt
     9661 tgaagcaaaa gaccgaaaac gaagcgtatg aatacggcag attgtttgtt aaagaactga
     9721 tgcataagga atgcaaccgt gaaagccttt gaagataaag ccttggcggt gtccgcggac
     9781 atggcggacg cgttagcgga cggcggggct gcggcaaccg ccgtaggctg cccccctagg
     9841 ctaatagggg gggagcaaaa taaaacccca aatccgaagg gtgcggaaaa aacagaaaac
     9901 caagaatttc aattcgagta tttcagtcat tttgtatcgg acggcaaagg cagatttata
     9961 gaaataccgt taaggcgtgg cagggatgac ggagcgttca tagaccaaat cactttcacg
    10021 atacatgaaa attccatgac gaaagtaacc ggaaagggat tggtatcaga tacggaattc
    10081 gttgtccggt atagcgagct gcttgaagaa atcttgggat tcggcattac caaaaaatta
    10141 ccgtttaaag gaaagttttt ctatcaaagc tgttatcaat tcgggcccga taacgtcgaa
    10201 tacggaaagg ttcattacgg cggccaatgt gaaacgatgt tggttgaatt gaacggtaca
    10261 ggctgcatgg ctgctttacc cggttgggaa aaccgattgt atgaattttt aagtaaatgc
    10321 gtccgaccca aaataacccg tatcgatgtc gcccatgatt ttttcaacgg agagtacaca
    10381 cccgatcagg caatgttaga ccacgataac ggacattacg acgtacacaa tatgcggcca
    10441 aaaagcgaat gtcgcggcac ggcatggcgc aatgaagacg gcagtggaaa aactttttac
    10501 atcggcaagc gcgaaaattc aaaatttacc cgtgtttacg agaaaggcag acagtttgga
    10561 gatgttgata gcccttgggt caggtttgag ggggatatag aaataccctt ggatgtccta
    10621 ctttattcgg ggtcgtatct aggcggcgca tatccgattt gcaaggagat ttttaagaca
    10681 gaagccaagc gcatggaagt aaaagttaaa aacgttaatc tgatattcga cgttaagctt
    10741 tttcatgcaa ggaatcaagt cggcaagatg gttaatttcc tccgcgatat agggtgggac
    10801 gatagcagga tagttgatga gctggtgaaa ggcgttgaag gttatccaaa aggtttacag
    10861 cctgagcaat acgattgcaa aaaccaaacg cagaaattcc agtatatcca cgaagaacag
    10921 aaagccatta acgcattgaa cattgaaaca ctgtttgatg atttgattga agagagagaa
    10981 tacgcatttc ctcaagacag ggaacggcaa cacatcaagg atattgagta cgaggaacgt
    11041 caaatatccg attttttaaa caactgaagg aaattgaaaa atgtttgaaa caagccaagt
    11101 aaccacatat accgccactt tgttgggcgc aaaaaaattc aaaggcgaaa tcgacggtaa
    11161 caaaatcgat tcatgcaccg tcttggtagc cagccccatg ccgtcaaacg gcaatgccgt
    11221 gggctttacc tcggaaagca tgaagtttgg tgacagccat aattttgaaa aattgaaaaa
    11281 ccttaaattc ccctgtgcgg ttgatctaac cgttgccatg gaatcaacag gaaaaggctt
    11341 agtgcagaaa ttgttggatt tccaagtaaa gggcgcagca cctaaagcct aagaaaggct
    11401 gaatcatgag cagataccaa cagaaattta tcgtacagga attggaaaat cacgaattca
    11461 tctatcccga ttcgtttggc gatattggat ttacgtcgaa catcaaatca gccggtaaat
    11521 atgacagcta tgaagacgct tttagctcgg cattggaaga gataggcggg gaatttgtaa
    11581 ttttcggatt ctacgaaaaa gaagattaag tttaagaggc tcggcaggcg gtctctaaaa
    11641 ccttcacaaa gcccgcaaac acattttttt aaaaatttcg t
//
LOCUS       DAWTHD010000072        11671 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWTHD010000072 DAWTHD010000000
VERSION     DAWTHD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.072"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            76..274
                     /locus_tag="WM704_001505"
                     /pseudo
     CDS             76..274
                     /locus_tag="WM704_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            350..517
                     /locus_tag="WM704_001506"
     CDS             350..517
                     /locus_tag="WM704_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340900.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            677..1522
                     /locus_tag="WM704_001507"
     CDS             677..1522
                     /locus_tag="WM704_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFA9340901.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGYQITPKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESIEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDAPPKAEPKLPIDGNSLADIAAMVYYGTWMIESGKD
                     ISAPLKQLGCRQAVTMWTVWHETRPILKRSAATLEVLRGYADKDASDRIAACLEGIYG
                     KATVR"
     gene            complement(1559..1708)
                     /locus_tag="WM704_001508"
     CDS             complement(1559..1708)
                     /locus_tag="WM704_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340902.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADYREML
                     KRYAA"
     gene            complement(1711..2385)
                     /locus_tag="WM704_001509"
     CDS             complement(1711..2385)
                     /locus_tag="WM704_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340903.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(2382..2867)
                     /locus_tag="WM704_001510"
     CDS             complement(2382..2867)
                     /locus_tag="WM704_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340904.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(2873..3346)
                     /locus_tag="WM704_001511"
     CDS             complement(2873..3346)
                     /locus_tag="WM704_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340905.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(3401..4696)
                     /locus_tag="WM704_001512"
     CDS             complement(3401..4696)
                     /locus_tag="WM704_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFA9340906.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELAHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(4717..5229)
                     /locus_tag="WM704_001513"
     CDS             complement(4717..5229)
                     /locus_tag="WM704_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340907.1"
                     /translation="METADGSDNSLFGDFSDEDVKKGVEKLIQEKLAGYEADMKRQEA
                     AKAHYREIYTAHPDADSIVESRELEEWLAGQNPLVRKAFNDALKDGTAAEVIGAFDMF
                     KAAKSAAEPEKPAEKPPAGKNTPNTLSDIPAGRDHTASDGPPDYLSGNALAEKLASMT
                     EEQVEKFLNS"
     gene            complement(5322..11256)
                     /locus_tag="WM704_001514"
                     /pseudo
     CDS             complement(5322..11256)
                     /locus_tag="WM704_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
BASE COUNT         2463 a         4166 c         2769 g         2273 t
ORIGIN      
        1 gcccgaccgc cgccaagccg caaacttggc ggcttcttta tgccgtctga aaccatcttc
       61 agacggcatt tttctttgat tttgttgggg gaatggttga ctttgaacgt gtccacgttt
      121 ataattcaat tcatcggcgc atcggatgcc gatacgggaa aagccccacc gaagcggggc
      181 ttgtacaggg agtaggaaaa tgaagctcat ccttcaaatt ctcatcctgg ttttaatctt
      241 gatgctggca ggcgatacgc ctattagcgg ataaaaccgg ctggggcggt agccgccgcc
      301 ccagtcccta tcttacaaaa ggcagttcaa aaaatcaagg agtttgccta tggttaatga
      361 aaaactggca gaaaaccgaa agcgttacga gcaaaaacga gtcattaaaa aagtctcgtt
      421 taatgcggaa acagaaaaag agcttttgga atatgcccaa aatctcgatt tttcccaatg
      481 ggttaaatca atcataaaag aaaaaatcaa aaagtaattg gctttgaacg tgtccacgtt
      541 tattattctt acaacggcac agccgttaat ggaaaaccga accaagcggg aacttggttc
      601 ggttaagtga aaagcagttc taggaaaact cttttcgcgt cttttaaata acctctaaaa
      661 gaaagactat tctatcatga atgctgtcca agttttaaac tttcaacaaa actccgtccg
      721 tactgttgcc gataacaaag gcgagttgtg gtttttggca aacgatgttt gcgagatttt
      781 aggctacacc aaccctcgcc gaacggttga cctgcactgc aaaagcaggg gcgtaacaaa
      841 acggtacacc cccaccgcca gcggcgaaca ggagatgacc tatattaacg agccgaatct
      901 ctaccgcttg attatcaaat cccgcaagcc tgccgcagaa gcgtttgaag aatgggtaat
      961 ggaaaccgtc ctgcccgcca tccgcaaaac aggcggctac caaattactc ccaaaaccac
     1021 cgccgacgac cgtaccggat tgcgccaagc tgttgccgcg cttgtcggac gcaaaggtat
     1081 agactactcc tccgcgtaca gtatgataca ccaacgcttc aacgtcgaat ccatcgaaga
     1141 cctccctgcc gggaagctgc ccgaagccgt cgcctacgtc cacgcattga cactgcacac
     1201 gggcttgaca ggcgaagtgt tggacgcacc gcccaaagcc gagccgaaac tgcccatcga
     1261 cggcaactct ttagccgaca ttgccgctat ggtttattac ggcacatgga tgattgaatc
     1321 gggcaaagac atctccgcgc cgctgaagca gctcggctgc aggcaggcgg ttacgatgtg
     1381 gacggtttgg cacgaaaccc gtccaatcct gaaaagatcc gccgcgaccc tcgaagtgtt
     1441 gcggggatat gcggacaaag acgcctccga ccgcatagcc gcatgtcttg aaggcattta
     1501 cggcaaggct acggtaaggt aaacggaaaa tgccgcccga atcttcggac ggcatttttc
     1561 acgcggcata gcgtttcaac atttcccgat aatccgccgg ttcccgtccg gcgaactcaa
     1621 aggccttcaa atcggcgtta tctgatgcct tgcggctctc gttcagccaa taggcgatct
     1681 gtttcctgca aaactcttca gcactcatcg tcaaatccaa aaatgcctgt tcaggtgcgg
     1741 cgcatcctca cgcgtgatac ggcgcaaatc actgtcggcc ggcaggccga aatacagctc
     1801 gaaacgcctc cttgccgatt ccgaacgctg cgggtcgaaa gtctccgcat ccgggatact
     1861 gaaagcctcc gccaaagccc aataaaccaa attccgatga tgtatggaat tgatttccgg
     1921 ctcgtccccg tcccccgcca tatcccgcgg caggcaatac cccccggcaa acaacctgcc
     1981 gtcacggtcc ggcgcaggcg caagcgtcag cttcccgtcg ccctgcaccg cataaaccgg
     2041 cagccccgtt tgcgccccgt cgcgccaacc cggggcgcaa acatccgccg cctccggcga
     2101 aaccaacttc aggcacaccg gcttgccgtc ggacacaaaa cggatactgt cgatttcata
     2161 cgcataaccc gccgcaaacc cccctgccgc ataagcataa agcggccgcc ctttttccac
     2221 atccgttaca aacgcatcgt ccgaatgcag cagccggccg cgcaggcacg cctcgtgaac
     2281 cgcatcgttc aaccatcccg acacctcttc gtcgctccaa aaatacggct cggccttatc
     2341 attggcaagc acgcggaaac gcgcaatcaa agccttcaaa ttcattgcgc gataccgaac
     2401 tgacgaacca tccgaaccac gtccccgcgc aaattctccg ccgatttgcg cttatccaaa
     2461 ccctggccgt aattcgcctt cgcatacagt tcaagctcgt ccttgcccat ccggttgatt
     2521 tgcaggatca catcctgcac cgcgtcaaac gccgccgttt cccgttcgag ttccgaagcg
     2581 cccgcctttt ccaaagcctc cgtatcatcc gccttttcca cgcgtttgcc cgggacggcg
     2641 gcaaacacat cgccgtggcg caacaacttt gccgcagccc cggccgacac ctcgcgcacc
     2701 tggccgtctt cgaaaaccaa gcccgtatga tagatacggt cgcgccacac ggcacgcccg
     2761 ccgatatact tgataaaaac cttatccatc caaccccccg ccgccgtccg gacggcattg
     2821 tcaaatacag caacgccgca aaggcaaatc cccgggcggc gtttcattcc gcttacaact
     2881 ggccgaactt ctcgccgtaa atcaaaatat cgatatcggc ggccttctta ttttccgccc
     2941 ctttaagcgt aaccgtcagc aaagcctgct tgggcagcgt aaccagtttg gcagactggc
     3001 agcggatacg cccggcagac gcaaagtccg cgcccgaagc aaaataagcc gcatcctgag
     3061 gcactttcgc atcatccgca ccatccgcat acgcaaagcc gacatcggca gtgataccgg
     3121 aagacatcgc cgtcttcaca ataatctgcg cgtcatccaa acggaaaccc tgttccaaga
     3181 cacccagcgc caacacatcg cccgccttag gcggctcaga agcatccgcc cccaagatcg
     3241 caccgtcctg cttcgcaacg atacggtaat gctccgccgc cagattgccg taaggcacgc
     3301 cgccgaaacg gttgccgcca ttgtttttca cataaatctc agccataaaa aacctcaacc
     3361 caacaaatag gaaaagccgc cctttcaagc ggcattcgaa tcagcggata ccgccgtgca
     3421 aaggaaccac cgtatccaca accgtgacac cgtagtcggt aatttccttc gcgcccccgc
     3481 cgacatccac ggcaaagcgc gttttcgcca caccgcgtat cgtaccgacg agcgtttcca
     3541 cacggttgcc gtgggcaagc tccttctcag accaaaagaa aggcataccg ccgtgtttgc
     3601 cggtattcgc aaacgcctcc aacaccgcct ggccgcccaa aataaccgaa cggtcgaccg
     3661 caaacttgtc cgcaaacgaa gccgggattt tcaagcccga ttccgcctcg ctgaacttat
     3721 cggcgcaata cttcatctca tcgcccgcat agaaacggat ggggcgcggc attttcacca
     3781 ggataaagcc gttccacaaa cccgcatcgc ccaaaaacag cggattttga tttgcctggg
     3841 aggcgcgcgc gattgccgaa gcctgaagct ggcggaattt cggatcggcg gcaaaacggt
     3901 tgtactgcgc cggggaaagc agccacacgc gcaaaggcga atcacccgcc gccttgtcgc
     3961 cctcaaactt cacaatcggc ggcggcagcg gaatctggtc gagcacggta cgcatactgt
     4021 ccaccgtatc catcgtaaac aaatcggtag aggcaatctt caactcgccg ccgttgtccc
     4081 caacgcccgt taccgcatcg cccgaagccg taaaatggcg gtttttggac ggggctttga
     4141 ccggattgac cgcataatca ttgaatttcg ggtcgcccgc caaaggcacg ccccattcga
     4201 tattgtcatg aaaaccgcgc gcgcccgcca tatgcaccaa cagcgtctgg tcggcataac
     4261 ggtccatcag gctttgcgcc gccggacgaa tcagcgcgcg ataatcggca gggctgcgct
     4321 gattcgtcat cgtgccgccg ccgtcaacgg ggaaacgcgc ctgattcaca cgcaaacccg
     4381 cctccgacag gctcatcccc acgcccctgc cttccgccgt gttgtcgccc ataatcggca
     4441 gggcggaaac agggttgacc aaattgaaac ggatttcgtc acccatgccg cgcgtcaaat
     4501 cctgacagcg cacgaccggc atatgctggg tcgtctgttt gcgcaaagtc gcctccgcac
     4561 ccgcggtacc ggcaggcatc ttgcccgcca aacggttcag cgtactgttg cgctgcatat
     4621 gcatcgcaaa cagccccgcc gcctgcttca tcatcgcctg cgggtcgccg taggccgtat
     4681 tcgtcttttg tgccataaag agcctccctc aaaaactcaa gaatttaaaa acttctcaac
     4741 ctgctcttcc gtcatggaag ccagtttttc cgccagcgcg ttgccgctca aataatccgg
     4801 cgggccgtcc gaagccgtat ggtcgcgccc cgccggaata tccgacagcg tattgggcgt
     4861 attcttcccg gcaggcggct tttcggcggg cttttccggt tcggcggcgg attttgccgc
     4921 cttaaacata tcgaacgccc cgatgacctc ggcggcagtc ccgtccttaa gcgcgtcatt
     4981 aaacgccttg cggacaagcg ggttctgccc ggccagccac tcctccagct cgcggctttc
     5041 cacaatcgaa tccgcatcgg ggtgcgccgt atagatttcg cggtaatgcg cctttgccgc
     5101 ctcctgccgc ttcatgtccg cctcataacc cgccagcttc tcctgaatca gcttttccac
     5161 gccctttttc acatcctcgt cggaaaaatc cccgaacaac gaattatcgg aaccgtcagc
     5221 cgtttccact ccgggttttt ccgccccctt ctcaagctcc tcgatgcgct ccctcaaggc
     5281 ttgggcttcg gctttcgccg acttttccgc ctcgcgcgcc tctaccagct tttcataagg
     5341 gatagtgtgt ttgccgtcct tggcacggat gaccttgtcc gcacgccgtc cgccgcgccg
     5401 atacccgcgc cgaccaccgc gcccgtactg ccgaccacca gcgattccgc ccacttctta
     5461 ccctccaaac cgtcttggat atcggggaaa accaactggt ccaaacccaa acgccccgac
     5521 acgtccagcg gcgtcaggcg gctgaacccc ttggccaaca cctcgcccgc cttatccccg
     5581 aaagtgtccg ccaacatatt gcgcaacgcc gcttccgcat cccacgggtc atcgtcgtca
     5641 ctgcccaaca tcgaaaccac cgcaagcagc gtcgacacaa acggcagccc caagatgccc
     5701 gaagccatcg catgcgagac caacagcccc gccagcgtct tacgcgcctg cttatcgccc
     5761 ttaaacgcaa ggtacgcatt gcgccccagc gcatacagga tattctgcga atactgcttg
     5821 aacagaaaga ccaccttcgc cgcattgccc atcataaagc gcggacggtt ttgcgccgca
     5881 tagtcaaaat gcccgtcata cgtcgccttt ttcgcctgtt cgaaagccgc ctcgctgtcc
     5941 gcccccgccc gtttcgccaa acggtaggcc gcgacaaacg taacctggcg gttaaacttc
     6001 tccgcatgat ggaacagcca ggccgcctta tccatcacct tctgccaagc cgaccccgcc
     6061 aaccccgggt cgccgttggc cacccccgcc aaatcatgcg cctgcgacaa atcgatcaca
     6121 ccgtaatccg ccgcctttcg gaacgccgcc ttctcatccc cgttcaaact gtcctcaatc
     6181 gtattgaact tctccccgaa cctcagcccg atctgttttg aagcccgcag caattcccgc
     6241 gccgcaccgg cataacccca cttcgccgcc atcaccggat aagccaccat tgccgtctgc
     6301 gacaggttca caaccgcaga agcgggcgac atccccatca tccacaaaaa gccgaagccc
     6361 gtcaaagcct gcgccagctt ggaaggattc ggattcatca ccgcctcgtg gcgtttcctc
     6421 atctcatccg ccacacgctg caactgacgc tggtcgaaac cctcctcata tttgcgcccg
     6481 tccacaaaat cctgcatcac atccaactgt tccgccatac ggtccgcata gcgcaacttc
     6541 gccagataat tcgcaccgct gcccatattc tgcgcatatg cgcgcctcgc atcatcgctg
     6601 aagcccggca cgcccttgcg gtggatgccg tgtttcgccc aagacgtatc gggcagcgag
     6661 ttcaaataaa gctgcgtcag cgtatcgttc aatcgggccc gttgcgccgg atccaaatcc
     6721 atcctgccga cagcctcgcc cagctccctc ataaaaccgc cgcttaccgc atcgcggctt
     6781 cggatataat cccgggactt catcacgggc gaaaccttaa accccgcccc gaaatcagcc
     6841 ttcagcgcat cgcgcagctt ctccgcctcg ctcaaagttt ccgcgcggga cacattcgca
     6901 ctattcccgt ccgcatcctt gaccaccacc agataatcgc cgaaacgcgc caaggggaaa
     6961 tacacaccgc ccagctcgtt gtcaaaacgc tccttcaggc ggcgcaccgt ttccgcatcc
     7021 tgccccgcac gcgccaaccg ttcggcaagc gcatcgcgca cctgcccgaa atgcgcccta
     7081 taatcatccc gcgccttgcg gtacaccctc tgcgccgcat caggcagccc ggcaaaacgg
     7141 cggttcagca aagcaagccc ctccaaagcc ttttgcgccc caacctcatc cttttccaaa
     7201 cgcttcacat ccgcgcgcgc ctgctccgac atccgtaccg catccgtttt agcccggctt
     7261 tccgccgcca catccgcccc ggcgcgcctc aacgcccgct tcgcctccga atccgcataa
     7321 aacagacggc gcaggcgcat atccgcctca tccgccaaaa tttcccgacc gtgtttttcc
     7381 tgcgccttct ccagcgcata agccgcctta tccgccgccc gttgcgcttt ccgtaccgcg
     7441 gcatccgcac gcgcggtacg cgcaccggcg gaagcaacgg ccgcctccgc cttcgcgatt
     7501 ttaccgtccg caatatccaa agccgtccgg atgccgtcca aacgcttctg agcatccttt
     7561 cgcatcagcg ggtcggcatc caccttcgcc agcgtcgcat cgtgcatcag atccgcaagc
     7621 gctttcgcat cctcctcctt caacctgccc cattccctga caaccttgtc cgcctccgta
     7681 accgccttgt tcgcatccgc gccgaagagt tccgacaaac gcccgtattc cttcaggccg
     7741 tccaaaacgc cgccgtaaat gtcctcgatc tgccgcctgc ccaaaaactg gagctgaatc
     7801 cccttggaag catcccagcg gtctttcagc gcgtttgcca aatcggattt tttacccgtt
     7861 atatgaccga ttgcggcccc gatgttcgcc gcacgggaaa aacgcaccgc ctccgtgtca
     7921 aaatcggcaa tgccgccctt gcccgcatct ttgtcttgca aaatctgcaa caggcgttta
     7981 tcatcaatgc ccgataaagg cgatttgctt tcagaggctc ccgtagttga tacgcggtca
     8041 agtaatgaaa gcaagttgcc tttttctatt ttcgtcagct tatggtcgta atagcggtta
     8101 ccgttcctgc taaccgcgac ggcagccctt accgtgtaat ccgcaccgcc gacattcagt
     8161 cccgaaacat aatattcata accctgaata tcgccgttct tagccaaatc ctcgttcggc
     8221 agcgtatcga tatacaccgc attctcaata atctgcggaa ttgccgcgat actctgcaaa
     8281 tgttccgcgt ccttatagtc gtgacgcaat atttcagtga tgcccgaacg tcccaaactg
     8341 atttcacgcc ccgtgtcctt attcacataa ggcccgcgca aagatttgcc gtattccagc
     8401 gcattgcgct tatattcgcg caaattgccg ccttccggaa cctccctgcc cgagatacgg
     8461 atggtttccg cacggcgcag cttctcaagg ctttccgact tggaacgttc cgccgcaccg
     8521 tcttcggccc gcgcgaacat cactgcacga tgattgccgc cccaaggcgc atcccgcgcc
     8581 ccttccacat tcccgtgcaa atccgccagc atcgcaaaca catccgcgtc cgacatcgca
     8641 ccgccggcct tgcccgccag gcgttgcagc acacggcgca acagggcgcc gatacgcgcc
     8701 aacacgcccg caatgccgcg tttcaaaccg ttgccgacct tcacgccgta acggttttca
     8761 atgcccgccc aaccgccggt acgctgcgcc gcataaagct ccgcgaccgc ctcttccacc
     8821 gcggcggggc gcaccgaggc cgccgcatcg cccgtgcctt cgcgcccttc ctgcaccgcg
     8881 tccgcaatgc gccgaatcag gccgttgccg tccgcacgtt ccaattcttc acggtacttg
     8941 gcgaaaccat ccgccgcaaa gcccctgtgc cccagctcgt gccacgccgc ccataccgca
     9001 cgttcgggcg taaggttttc cgccaccagc gtgattttgc ccgtcctgcc gtcaaaccag
     9061 ccctccacac gctgcgacaa cagcatctgc gccttgtccg gcgccgtttc ggacacggcg
     9121 gcgacatcca cccgttccgc cagtccgcct atgctttcgg cgaggcgttt tttgatgctt
     9181 tcggcgtgct cgcgcaatgc cttaaaacct tgcggcgcct gacgctcttt caccgtataa
     9241 acaggccctc cctgcaacac acgcgccttg cgccgtgcct cttccggatt gtccgaagcc
     9301 gttttcgcaa acgtttcagg cttggctttg cccgtctttt cagacggctt ttcggttgcc
     9361 gcgccttgtg cggcggccga cttgcccttt ccgccatcga agcgggattt tcccgcttcg
     9421 atatttcgca ccttatccga agacggcttt tcggttgccg cattttttgc agcagccgcc
     9481 ttgcccgcaa caggcgtatc ttttgcccgg gcaggctgcg ccctgcccgc cggtttgccg
     9541 tcggcggcgg catcgcgcgg ccgcgccatc accttggccg gcgcccttgc ttcctgcgtc
     9601 ggacgataat cccgacccgg ctcggacggc acggcttccg acaaaccgcc cgcccgcaag
     9661 gtttccgcct cccgcccctt caaataacgg cgaccgtcgg agacaaggcc gtcagagttg
     9721 gaaggaccgg ccatatcggc aaactcctcc ggcatgccgc ctttgcccgg cctgcccggc
     9781 acgcccgcaa gcgcatcaat atgcctgcgg tccgtatgct cgaaacggta ttcgccgccc
     9841 cccaaaggca cacccgcctt ttccggcctg cgcctgtccg ccgccaaacg gcgcgtgaaa
     9901 tccgccgccg catccgccat ccgtttctta ggcgataccc tgcccgccga ttccgcaacc
     9961 tgcggattca ataccggcaa caccgtatcg gggcgtttgc ccgcaacagg cgcacgtttc
    10021 ccgccgtccg tcccggcaag gttttcagac gcggacaggc cgggcgcggc accttccgcg
    10081 cgcgcccgaa cctgtaagtt ttgccgacgg gccggcgcat tgccggacgc aacggcggac
    10141 cctggcaaac cctgcaaggc agccgcattt gcatccgccc ccgcatcagg ggtcaaaccc
    10201 ctgccgcctt gggtcaaacc cctgccgcac atcctgccgc acatcctgcg ccaaaccctt
    10261 atcttccgtg ggtaaagccc gggtcaaacc cctgccgccg ccgacaacgg cctcagacgc
    10321 gccgtcatcc ggcaacgccg tccgaacccc ctgcaaacgc tccccctcgc ggctcaacag
    10381 cggcacgctc caaccgttgt tgcggcggta accgtaaacc gcacggttaa gcctgtccaa
    10441 cacatccgcc gattccggat gttgcgcacg gtaagcgcgt acagccccgg ccgattccgc
    10501 caaaccctca ggataatgtc cgcgcatatc cgccataccc gcctcccggc gcataatccc
    10561 gtccgccgta tagtccgcca ccgtctgcgc atgacggcgc accccttccg gcagcgcgtc
    10621 aaaccgcctg cgcgattcct tatccaacgc atcggcaggg acggaaaacc cttttttagg
    10681 aacaggcacg gcatccgcca tacgcacggg cggcggcagg taaccctgtt tggcttcgga
    10741 agccgcctga taatccgcct caatccgcgc gtgatacgcc tgctcgattt ttcggcgcac
    10801 aggcgcggcg atttgcccga aatccgcaac gcccttaata tcaaggcccg cctccttcgc
    10861 caaagccttg cgcccccggg tatccaagcc gtcgaaatat tggcccgcag gcagcggcga
    10921 gacccgcgca atcccgcccg aagcggcggc ggcagaaccg cccggcgcaa ccgaagccgc
    10981 accgcgcggc gcaccgccgg ggcgcacatt gccgtaagcg gacggaacac cgcccctgtc
    11041 cgcatccgcc gaccgtccgg acggcacatc atccgcaacc gaccgggctt ccatatcgtc
    11101 agacaccgca gacggcacag ccgcgcccga atccgccgcc aaagcagcgg ccgcagaaac
    11161 cgcaccgtca tccggattaa tccccatcgc ctccgacggc ttggcccggt attcggccgc
    11221 catgtccgca tactgacgcg ccaaaccgtc cggcatcacg cccgtatcca aaaattccgc
    11281 cccgtcctcc gacgggatac ccgaaacatc cggctgtttt tccgtcccgc gcgccaattc
    11341 ctccgcgcgg ggcgtattgc cgtcggcagg gccgctgaaa aaccgccccg cctcctcctg
    11401 cttcgtacgg tccgcaaact gccgcaaagc cggatgcgac tgatcgtgat gcgccgtatc
    11461 aaaacccgcg ccgccctgtt ccgcgccgcc gtccgccgcc gcacgcaacg cctgcgcccc
    11521 ttccggggcc ggcctgcccg ccttcctgtt gcccccgata cccccctgcg cacccgcctt
    11581 ccgtccggcc ggcggcctga gcgggtcagt taccgccgca taagccttcg ttccggcaaa
    11641 ccccaccgca gaatgcccca aagaagagaa a
//
LOCUS       DAWTHD010000073        11584 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWTHD010000073 DAWTHD010000000
VERSION     DAWTHD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.073"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            160..876
                     /locus_tag="WM704_001515"
     CDS             160..876
                     /locus_tag="WM704_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFA9340908.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            1025..1927
                     /locus_tag="WM704_001516"
     CDS             1025..1927
                     /locus_tag="WM704_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFA9340909.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1970..2744
                     /locus_tag="WM704_001517"
                     /pseudo
     CDS             1970..2744
                     /locus_tag="WM704_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            2856..4544
                     /locus_tag="WM704_001518"
     CDS             2856..4544
                     /locus_tag="WM704_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFA9340910.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPGAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            4636..5592
                     /gene="gshB"
                     /locus_tag="WM704_001519"
     CDS             4636..5592
                     /gene="gshB"
                     /locus_tag="WM704_001519"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFA9340911.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKEFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GKVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            5751..6134
                     /locus_tag="WM704_001520"
     CDS             5751..6134
                     /locus_tag="WM704_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFA9340912.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            6266..6739
                     /locus_tag="WM704_001521"
     CDS             6266..6739
                     /locus_tag="WM704_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFA9340913.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            6864..7967
                     /gene="mnmA"
                     /locus_tag="WM704_001522"
     CDS             6864..7967
                     /gene="mnmA"
                     /locus_tag="WM704_001522"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFA9340914.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            8038..9708
                     /locus_tag="WM704_001523"
     CDS             8038..9708
                     /locus_tag="WM704_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFA9340915.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            9820..11454
                     /locus_tag="WM704_001524"
     CDS             9820..11454
                     /locus_tag="WM704_001524"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFA9340916.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2828 a         3150 c         3100 g         2506 t
ORIGIN      
        1 cctcgccttg ccgtactatt tttgtactgt ctgcggcttc gtcgccttgt actgatttaa
       61 atttaatcca ctatacttta atttctccta cacgatttga aacacagccg tcttaaaaca
      121 tcttaaaaaa tttgaaagcg taaaaatacg gaaaacatca tgaactttga aaacgacgac
      181 attatccatg cgccgaccac gtcttccctg attctcgaag agcggcacga ttcggagctg
      241 ttccgtgttt acgccctgat tttggacggc attaccgatc aggtgctgct gcccggcaaa
      301 aagctgaccg aatccgaact ttgccgtcag atggtgtgtt cgcgcaacac cgtccgcggc
      361 gcgctgtcgc ttttggcgca cgacaagatt gtcgatttgc aacccaacag gggcgcgttc
      421 gtccacgttc ccgatttgaa agaaatgcag gatgtgttca atgcgcgcat cgaaatggag
      481 acgatgattt tgaacatcct cgccggcctg ccggatttgg aaacgcgcct caagccgctt
      541 tatgcgatga taaggtgcga agaagaggcc tccggcaggg gcgaccgcgt cggctggaac
      601 cgcctgtcca atgccttcca cgtcgaactg gcgcgcctgg tgggcaacga tgtgctgttc
      661 gacattatga acacgctgtg cgcgcggtct tccctgattg tcgccgtggc gggcgtgcat
      721 cgcgagaaaa aacacgccat caatacgcat acgcattccg aacaccgcga aatcctcgac
      781 ctgctgctgg cgggcaggcg caaccgcgtg gtcaaaatcc tgcgccgcca tttgggcaac
      841 tgtatggagc gtttggaaaa gactttggaa gattgaatgc cggagcggat aagccgcctg
      901 aaccttcagg cggtttttta atggcgaacc tgatgccgtc tgaaatatgg atgcgggtat
      961 ctgcaatttt cagacggcaa tttttaagcc gcacatatca tgcggcaata aaggagggta
     1021 ggggatgagc agcctgatga cgttgttttc ggtattggta ccgatgtttg ccggattttt
     1081 tatccgtgtt cccaagcctt acctgcccgc ttcggacaag gtgctgtcgg ttttggtgta
     1141 tgccgtgctg ctgctgatcg gcgtatcgtt gtcgcgcgtg gaggatttgg gttcgcggtt
     1201 gggcgatatg gcgttgacgg ttctgtggct gtttgtttgt acggtagggg cgaacctgct
     1261 tgccttggca gtgttgggaa agttgtcccc gtggcggata gggggaaaag ggaagggcgt
     1321 ttcggtcggc gtgtcgggca gtgtgaggca gctcggatgc gtactgatcg gttttgtgtc
     1381 cggcaaattg atgtgcgata tttggatgcc gtctgaaaac gcgggtatgt actgcctgat
     1441 gctgctggtg ttcctcatcg gcgtacagct caaaagtagc ggcgtatcgt tgcggcaggt
     1501 tttgcttaac cggcggggca tccggctgtc ggtttggttt atattgtcat ctctttcagg
     1561 cgggctgctg tttgccgcat cggcagatgg tgtgtcgtgg acgaaaggtt tggcgatggc
     1621 ttccggcttc ggttggtatt ccctctcggg tttggtaatg accgaggctt acggggcggt
     1681 atggggcagc atcatgctgc tgaacgattt ggcacgagag ctgtttgcac tggcatttat
     1741 tccgctgctg atgaagcgtt ttccggatgc ggcggtgggg gtcggcggcg cgaccagtat
     1801 ggatttcaca ttgcccgtaa ttcagggtgc gggcggtttg gaagtcgtgc cggtagcggt
     1861 cagcttcggc gtggtggtca atatcgccgc cccgtttctg atggtggtgt tttccacgct
     1921 gggctgaacg cggtaaaatc ggcatcccga tgcaaggaag cagaaaacga tgaaaccgaa
     1981 aatccaaagg catggagaga ttttaagcct tgtccgccgg catcagttta tgtcggtgga
     2041 cgagcttgcc gccgcattgg acgttacccc gcagacgata cgttgcgaca tccgcgagtt
     2101 ggaggagggc ggcagcctga aacgccatca cggcggcgca tcttcgggcg gaaacttgcc
     2161 ggagggtctg cccgccgacc gccaaacccg gtgtcaaaac gaaaaaaacg ccattgcccg
     2221 gctgattgcg gaacacatcc ccgacggttc gtcgctgttc gtcagtatcg gtacgaccat
     2281 ggaagccgcg gcatcagagc tggtgaagcg gcgcagcagc ctgcggatta ttaccaacaa
     2341 tatccacgtc gcctccgtcg tttcggcacg tacggattac acggtcatca tcacatccgg
     2401 cgtcgtccgc cctttggacg gcggtattac cggcgtggcg accgtcgact ttatcaacca
     2461 gttcaaagtc gattatgccg tgatgagcac gcacggcgtg gaaagcgacg gttcgctttt
     2521 ggattacgat tacaaggaag tcagcgtcat gcaggcgatg attgccaacg cgcgcgtccg
     2581 ttttctcggc gtggatcaca gcaaattccg cagcaacgcg ctggtcaggc tcggcggcat
     2641 taccggcgtt tgacaaagta tttaccgaca ggttgcctga taccgcgatg cagaagatgc
     2701 tgaaagaggc gggggtggaa tgcctgattg ccgatgccgt ctgaacgcta tgtcaaagcg
     2761 cgcaagtcgg gtacaataaa cacatcatca aaccgcttca gacggcatac ggaacctccc
     2821 caatgccgtc tgaagccatc tgtttaaaga aaaccatgct caataaagac caattcgcgg
     2881 acaaccattt catccgcacc atcatcgaag acgacctcaa aagcggcaaa cacgaagccg
     2941 tccaaacccg ttttccgccc gaacccaacg gctatttaca tatcggacac gccaaatcca
     3001 tctgcctgaa cttcggtttg gcgtatattt acgacggttt gtgcaacctg cgtttcgacg
     3061 acaccaaccc ggaaaaagaa aacgacgaat acgtcaacgc catcaaagaa gacgtcgagt
     3121 ggctcggttt ccattgggcg ggcgaaccgc gtttcgcttc cgactatttc gaccggcttt
     3181 atgactacgc cgtcggttta atcaaagacg gcaaagcgta tgtcgatgat ttgacgcccg
     3241 aagaaatgcg agaataccgc ggtacgctga ccgaagcggg taaaaacagc ccttaccgcg
     3301 accgcagtat cgaagaaaac ctcgacctgt tcacgcgtat gaaaaacggc gagttccccg
     3361 acggcagcaa aaccctgcgc ctgaaaatcg acatggcggc aggcaacatc aatatgcgcg
     3421 atcccgtcat ctaccgcatc cgccgcgccc atcaccacaa caccggcgac aaatggtgca
     3481 tctacccgat gtacgattac acgcattgca tttccgatgc catcgaaggc atcacgcatt
     3541 ccttgtgcac gctcgaattt gaagcgcacc gtccgcttta cgactgggtg ttggacaaca
     3601 tccccgcgcc gcacgccacc cgtccgcgcc aatacgagtt ttcccgtttg gagcttttgt
     3661 acaccattac ctccaaacgg aaattgaatc agttggttgt ggaaaaacac gtttccggct
     3721 gggatgatcc gcgtatgccg accatttccg gtatgcgccg ccgcggctac acgcccgaag
     3781 gggtgcgcct gtttgccaaa cgcgccggta tttccaaatc tgaaaacatc gtcgacatga
     3841 gcgtgttgga gggtgcgatt cgcgaagagc tggaaaactc cgccccgcgc ctgatggcgg
     3901 ttttgaaccc gctcaaagtg accctgacca actttcaagc cggcaaaacc caaagccgcc
     3961 gtgccgcgtt ccatccgaac cacgaagaaa tgggcgatcg cgaagtacct gtttcacaaa
     4021 ccatctacat cgaagccgac gactttgccg aaaatccgcc caaaggcttc aagcgtctga
     4081 ttcccggcgg cgaagtacgc ttgcgccacg gctatgtcat caagtgcggt gaagtcgtca
     4141 aagacgaagc aggcaatgtg gttgaactca aatgcagcat cgaccacgac accttgggca
     4201 aaaatccaga aggccgcaaa gttaaaggcg tgattcactg ggtttccgcc gaacacgccg
     4261 ccgaaatcaa agtccgtctg tacgaccgcc tctttaccgt cgagcgtccc ggtgccgtgc
     4321 gcggcgaaga cggcgaatac ctgccgttta ccgatttcct caatccggaa tccgttaagg
     4381 aaatcactgc ttacgccgaa cctgccgcga aagatttgcc ggcggaaagc cgttggcagt
     4441 tcgagcgcat cggctatttt gtgaccgacc gccaagacca cggcaaagac acgccggtgt
     4501 ttaaccgcac ggtgacgttg aaagattctt ggcagcctaa gtaaacccca tccttgccgt
     4561 ctgaatattg ttcgggcggc atttctcctt tacccgcgaa atgcggcaca ttcggcacac
     4621 cggaaaggaa atatgatgaa agtcctcttt atcgccgacc cgatggcaag tttcaaaacc
     4681 tacaaagaca ccacctacgc gatgatgcgg gaaatggcaa aacgcggctg gcggctgttt
     4741 cataccttga gcggggaatt gtctgtaaac ggcggtttgg taacggcaca ggcatcggca
     4801 tttgaatttt cgggtgcaaa aaacgatgat gaccatgaat ggtttaaagc ggcggacaaa
     4861 gttcagacgg cattaaaaga atttgatgcc gtgattatgc gtaccgatcc gccgttcgat
     4921 atgcaatacc tttactccac ccaattactg acgctggcgg aacagcaggg cgcgaaagtg
     4981 ttcaacagcg gacgggcgat gcgcgacttt aacgaaaaac tggcgatttt gaatttcagc
     5041 cgctttaccg cgcccacgct ggtaacgacc cgttccgccg atgtccgcgc atttttgaaa
     5101 gaacacggcg acatcatcgt caaaccgctc gacggcatgg gcggcatggg catcttccgc
     5161 ctgaccgaaa aagaccccaa catcggcagc atcctcgaaa ccctgatgcg gtttgattcc
     5221 cgcaccatta tggcgcaacg ctacattccc gaaatcgtac acggtgacaa acgcatcttg
     5281 attatcggcg gcaaagtcgt cccctatgct ttggcgcgta tcccgcaaaa cggcgaaaca
     5341 cgcggcaatc tggcggcagg cgggcgcggt gtggcgcagg aattggacgg acgcgaccgg
     5401 gaaattgcag agactctggc tcccgagctt aaacggagcg gcatcctgct ggccggtttg
     5461 gacgttatcg gcagcaacct gaccgaagtc aacgtaacca gcccgaccgg attccaagaa
     5521 attatgaaac aaaaaagttt cgacgtggcg gcaatgtttg ccgatgccgt tgccgcgtgg
     5581 tcggtacgtt aaaccgatgc cgtctgaaag gcttttgctt cgtaaccgct tggtttgttc
     5641 gggcaggcag ggttttccct cggccggcag ggatgcgctt aatgccgtac ggacacccct
     5701 ccgccccgtt ttcagacggc atatattgag gacattttga aaggataccg atggaacctt
     5761 cctcctacgc ggcagaaaaa aaaggcaaaa gcggcatcag gcgcgtcatc aacgcattcg
     5821 gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc cgtcaggttt
     5881 tgtggctgaa cgcgctgctg gtgtgcgcgg catttttttg ggtttccgaa accgccgtcc
     5941 gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc aacaccgccg
     6001 tcgaagccgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa cgcgccaaag
     6061 atgcaggttc tgccgcacaa ctggttgcca tgctgatgtt ggcggcggtt tggctgtccg
     6121 ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta gtatttatta
     6181 ttcacttaat ctacatcaaa tttccgagca gtatttgtat gtaagattaa gcacattccc
     6241 cgtctgatat taaaggagca ggaagatgaa aaaattattg gcagccgtga tgatggcagg
     6301 tttggcaggc gcggtttccg ccgccggagt ccatgtcgag gacggctggg cgcgcaccac
     6361 tgtcgaaggt atgaaaatgg gcggcgcgtt catgaaaatc cacaacgacg aagccaaaca
     6421 agactttttg ctcggcggaa gcagccccgt tgccgaccgc gtcgaagtgc atacccacat
     6481 caacgacaac ggcgtgatgc gtatgcgcga agtcaaaggc ggcgtgcctt tggaggcgaa
     6541 atccgttacc gaactcaaac ccggcagcta tcacgtgatg tttatgggtt tgaaaaaaca
     6601 actgaaagag ggcgacaaga ttcccgttac cctgaaattt aaaaacgcca aagcgcaaac
     6661 cgtccaactg gaagtcaaaa ccgcgccgat gccggcaatg aaccacggtc atcaccacgg
     6721 cgaagcgcat cagcactaat ctgctgaaaa tatttgaaat gccgtctgaa aaagcccggg
     6781 ctttcagacg gcatttttat gcccgccttt aaaatgtgtt aaaatccgcc tttaaaaacc
     6841 gccgtttcca agccatcctg cgtatgaata cgacatcaaa cacttccaat atcatcgtcg
     6901 gtctttccgg cggtgtcgat tcttccgtaa ccgccgccct gctcaagcag cagggttatc
     6961 aagtgcgcgg tgtgttcatg cagaactggg aaaacgacga caacgacgaa tattgcagca
     7021 tcaaacagga ttcgttcgat gccatcgccg ttgccgatat tgtcggcatc gacatcgaca
     7081 tcgttaattt cgccgcgcaa tataaagaca aagtttttgc ttattttctt caggaataca
     7141 gtgcggggcg cacgccgaat ccggatgtgt tgtgcaatgc cgaaatcaaa ttcaaatgct
     7201 ttttggacta cgccgtaggg cagggcgcgg ataccattgc caccggacac tatgcgcgca
     7261 aagaagtccg caacggcgtg cattacctgc tcaaaggttt ggatcgaaac aaagaccaaa
     7321 gctattttct ctaccgcctc aagcctttcc aactcgaacg cgcgattttt ccgttgggcg
     7381 gtttggaaaa acccgaagtg cgccgccttg ccgccgaatt taatttgccg actgccgcta
     7441 aaaaagacag taccggcatc tgtttcatcg gcgagcgtcc gttccgcgag tttctgcaga
     7501 aatacttgcc gaccgacaac ggcaaaatgg tgacgcccga agggaaaacc atcggcgaac
     7561 acgtcgggct gatgttttac acattgggtc agcgcaaagg attgggcatc ggcggcgcgg
     7621 gcgaaccgtg gtttgttgcg gctaaagatt tgacgaaaaa cgaactcatc gtcgtacaag
     7681 gacacgacca tccgctgctc tatacccgca gccttgtgat gaacgatttg agtttcacgc
     7741 tgcccgaacg tccgaaggca ggacgctata cctgcaaaac gcgttaccgt atggcggacg
     7801 cgccttgcga attgtgctat ttggatgatg aaaccgccga gctggtgttt gacgaaccgc
     7861 aatgggcggt tacgccgggt cagtccgccg tgctgtacga cgtcgacatc tgtttgggcg
     7921 gcggcatcat ccaaacgacc gacaaacccg tcatcatcac gcgataaagg taatgccgtc
     7981 tgaaacggtt ttcagacggc attgttccgc tcaattccac tttaaagacc gatacctatg
     8041 gaaaaaatct ggttagaaag ctacgagaag ggcgtcagtg ccgaaatcga catcacgcaa
     8101 tacaattccg tcagcgacgt attccgccaa agcgtggaaa aatttgcccg tctgcccgct
     8161 tttcaaaata tgggcaaaac gctcacttat gccgaaaccg gcaaactggc gaccgatttc
     8221 gcctcttatc tgcaaaacgt cctcaagctg ccgcgcggcg aacgtgttgc cattatgatg
     8281 ccgaacgtat tgcaatatcc gattgccctt ttcggtattt tgcaggcagg tttggtggcg
     8341 gtgaacacca atccgctcta tacgccgcgc gagttggagc atcagctgaa agacagcggt
     8401 gcgaccgcca tcatcgtttt ggaaaatttc gccaacacgc tggagctggt gctgccgcgc
     8461 acgcagatca aacacgtcat cgtcgcctcc gtcggcgaaa tgttcgggct gcttaaaggt
     8521 tcgctgatca atttcatcat ccgaaaaatc aagaaaatgg ttcccgaata ccgtattcgg
     8581 gaaaccgttt cctttcagac ggcattgaaa gagggggcga agcacgtttt ccaacctgtc
     8641 gcattaaacc gcgaagatac cgcattgttg caatacacgg gcggcacgac aggcgttgcc
     8701 agaggcgcgg tgctgagcca cggcaacatc tgcgccaata tgcttcaggc aaaagaatgg
     8761 attaaaaacc aattgcgcga gggaaaagaa accgttatcg ccgccctgcc gctgtaccac
     8821 atcttcgccc tgacagtgaa tctgatgatt tttgccaatg ccggctcgaa aattgtcctg
     8881 attgccaacc cgcgcgatat gaaaggcttt atcggcgaac tgaaaaagca gcgggttaac
     8941 gtatttatcg gcgtgaacac gctgtttaac gcgatggtca accggcccga tttcgccgaa
     9001 gtcgattttt caggattgcg gctgactttg ggcggcggta tggcgaccca aaaagccgtt
     9061 gccgaaaaat ggaaaaaaat caccggcacg cccatcgtcg aagcctacgg tttgaccgaa
     9121 gccagccccg gcgtgtgctg caacccctta aacatcgaat catacagcgg cagcatcggt
     9181 ttgcccgtcc cgtccaccga agtcgaactg cgcgacgcaa acggcaaaga agtccccgtt
     9241 gggcagccgg gcgaattgtg ggtaaaaggc ccgcaagtga tgcaaggcta ctggaaccgc
     9301 cccgaagaaa ccgccaaagc catagacgcg tgcggctttt tggaaaccgg cgatattgcc
     9361 gtgatggacg aaaaaggccg tctgaagctg gtcgatcgca aaaaagacct cgtcgtcgtt
     9421 tccggattca atgtttatcc gaacgaaatc gaggaattca tcgcgcacca cgaaaaagtt
     9481 atggaagttg cgtgtatcgg cgtacccgac gaaaaaaccg gcgaggcact caaagtgttc
     9541 gtcgtcaaaa aagacccgtc tttgaccaaa gaagaactca ccgctttctg ccgtaccggt
     9601 ctgaccgcat acaaagtgcc gaaagacatc gaattccgcg acgagttgcc caagtccaat
     9661 gtcggcaaaa tcctgcgccg cgagttgcgc caaagtgccg ggaaataaag aaaagatacc
     9721 gtctgaaaac agccgtccac cgttcagacg gcatccgtcc gtttgcaaga accgcgcgct
     9781 tcacgttaaa atcacgcatt ccaatacggg tattccatca tgaccaaatt cattttcgta
     9841 accggcggcg ttgtctcctc actgggtaaa ggtatcgccg ccgcttctat tgccgccatc
     9901 ctcgaatcgc gcggcttgaa cgttaccatg ctcaagcttg atccttatat caacgtcgat
     9961 cccggcacga tgagcccgtt ccaacacggc gaagtgttcg taaccgacga cggcgcggaa
    10021 accgacctcg acttgggaca ctacgaacgt ttcatcgatt ccacgatgac ccgccgcaac
    10081 agcttcagca cgggtcaggt gtacgaaaac gtcatcgcca aagaacgacg gggcgactat
    10141 ttgggcggca cggttcaagt catcccgcac attaccgacg aaatcaaacg ccgcatccac
    10201 gaaggcgcgg cgggttacga tgtggcgatt gtcgaaatcg gcggtacggt cggcgacatc
    10261 gaatcgctgc cgtttttgga agccatccgc cagatgcgaa gccagttggg acgcaacaac
    10321 accctgttcg cccacttgag ctacgttccc tacatcgccg ccgcaggcga aatcaaaacc
    10381 aagccgaccc agcacaccgt gaaagaaatg ttgagcatcg gcttgcaacc cgacatcctg
    10441 atttgccgta tggacaggaa aatgccggca gacgaacgcc gcaaaatcgc cttgttctgc
    10501 aacgtggaag agcgcgcgat tgtcggcagc tacgatgtgg acagcatcta cgaatgcccc
    10561 gaaatgctgc acgaccaagg catcgacaac atcattaccg agcagttgca gcttaacgtg
    10621 cagcaggcgg atttgaccgc gtggaaaaaa atcgtccacg ccgtcaaaaa cccgaaacac
    10681 accgtcaaaa tcgcgatggt cggcaaatac gttgatttga ccgaatccta caaatcattg
    10741 attgaagcct tgaaacacgc gggcatccat accgaaaccg atgtgcagat taccttcgtt
    10801 gacagcgaaa gcatcgagaa aaacaagggc gacgtttccg tactcaaaga tatggatgcc
    10861 atcctcgttc ccggcggctt cggttcgcgc ggcgtggaag gcaaaatcgc cgccgtgcgc
    10921 tacgcccgtg aaaacaacgt gccatacttg ggcatctgcc tcggtatgca gattgcgctg
    10981 attgaatacg cccgcgacgt ggcaggtttg aaaggtgcga attccactga gtttgacttg
    11041 aaatgcgctg cccccgtcgt cgccctgatt gacgaatggc aaaccgccga cggcagcgtc
    11101 gaaacccgtg acgaatccgc cgatttgggc ggcacgatgc gtttgggcgc gcaagaagtc
    11161 gaattgaaag caggcagcct cgccgtcaaa atctacggca gcggacacat tcgcgaacgc
    11221 caccgccacc gctacgaagt caacaacaac tacgtttccg cgctggaaca ggcaggtttg
    11281 gtcatcggcg gcgtatccgc cggacgcgaa cgcttggtcg aaaccatcga actgccgaac
    11341 catccttggt tcttcgcctg ccagttccat cccgagttca cgtccaaccc gcgcaaaggg
    11401 catcctttgt tcaccgcgtt tgtcaaagcc gcgttgaaca ataaaaaagc ctgataaagc
    11461 gttacttgat gatcaaaatg ccgtctgaaa gcctgacaaa gactttcaga cggcattttc
    11521 gcaaatcggg gatcataaaa gccatcaatt taatttgggt ctgtactaga ttagcagata
    11581 tgtt
//
LOCUS       DAWTHD010000074        11459 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWTHD010000074 DAWTHD010000000
VERSION     DAWTHD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.074"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            276..1580
                     /locus_tag="WM704_001525"
     CDS             276..1580
                     /locus_tag="WM704_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFA9340917.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(1674..2969)
                     /locus_tag="WM704_001526"
     CDS             complement(1674..2969)
                     /locus_tag="WM704_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFA9340918.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(3096..3983)
                     /locus_tag="WM704_001527"
     CDS             complement(3096..3983)
                     /locus_tag="WM704_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFA9340919.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(3983..4948)
                     /locus_tag="WM704_001528"
     CDS             complement(3983..4948)
                     /locus_tag="WM704_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFA9340920.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(4964..6088)
                     /gene="potA"
                     /locus_tag="WM704_001529"
     CDS             complement(4964..6088)
                     /gene="potA"
                     /locus_tag="WM704_001529"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFA9340921.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(6366..6635)
                     /gene="rpsO"
                     /locus_tag="WM704_001530"
     CDS             complement(6366..6635)
                     /gene="rpsO"
                     /locus_tag="WM704_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFA9340922.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(6840..7775)
                     /gene="secF"
                     /locus_tag="WM704_001531"
     CDS             complement(6840..7775)
                     /gene="secF"
                     /locus_tag="WM704_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFA9340923.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(7779..9632)
                     /gene="secD"
                     /locus_tag="WM704_001532"
     CDS             complement(7779..9632)
                     /gene="secD"
                     /locus_tag="WM704_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFA9340924.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(9844..10110)
                     /gene="yajC"
                     /locus_tag="WM704_001533"
     CDS             complement(9844..10110)
                     /gene="yajC"
                     /locus_tag="WM704_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFA9340925.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            10241..11359
                     /locus_tag="WM704_001534"
     CDS             10241..11359
                     /locus_tag="WM704_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFA9340926.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPGHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         2827 a         3034 c         2941 g         2657 t
ORIGIN      
        1 atcgcccaaa cctctgtata ttttcctaca gtaaattgtt gacaatccat acgcccgcat
       61 atgcgccgcc taaggataaa tcctcccgcc ggacaacggg ggcaagggat aggatgcgat
      121 atttccatat tcaaacaagg gatttgtttc acgcacaggg cggcacatcg gcaaaatccc
      181 cgcgccggtc cggcagggct tgcgcccatc ccggacaagc cccgaccccg cctttccgaa
      241 agacgggccc aaccattaag gaaactttaa tcaaaatgaa aaaacacata tgggcggcat
      301 ctttgctgcc ggcatcccta tcggcagaac ctttaaactg gtggaaacct tattccgccg
      361 tcaattcggg cgataccgcc tgggtgatga ctgcggctgc cttggtactg ttgatgacgc
      421 ttcccgggct ggctttattc tacggcggta tggtgcggaa aaaaaacctg ctctcgacga
      481 tgatgcacag cttttccatc gcgacattgg tgggcatcct ttgggtcgcc gtcggctatt
      541 ctttggcgtt cacgccggga aatgccttta tcggcggttt ggggcgcgta tttttaagcg
      601 ggatgcagat agacgctgcc gcacggatgc tgaccgtgtc gcccaatgcg ccgactgttc
      661 ccgaaccggt atttatgttt tttcagatga cgtttgccat tatttcgact gccattatta
      721 ccggcgcgtt tgccgaacga atgaaatatt cggcaatgat gctgttttcg ggcatatggt
      781 ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg cggcggcttt atgagcaagg
      841 gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat caatgccggt atcgcgggac
      901 tcgtcgccgc cttggttttg ggcaggcgca taggctacgg gcgcgaggcg atgcctccgc
      961 acaatatggc gatgacgctg attggcgcgg caatgttgtg gttcggctgg ttcggcttta
     1021 acgctggttc ggcgcttgcg gcagacgcgg cggcggggat ggcgatggcg gtaacgcagg
     1081 tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga gaaaatagcg ggacacaaac
     1141 cttctgcttt ggggctggct tccggcgcag tttccgggct ggtcggcatc acccctgccg
     1201 ccggctttac cggtccgtcg ggcgcggccg ccatcggtat attgactgcc gccgcgtgct
     1261 ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga tgattctttg gacgctttcg
     1321 gcatacacgg attcggcggg ctggtaggcg gaatattgac cggcatcttt ttcgacaacc
     1381 gcattttcgg tggggatgcg gcagtttggc agcagttgtg gatacaggta aaagacgggt
     1441 tcattatggc ggcatacagc gggctgatga gttgggcgat tttgaaggct gtggggaaaa
     1501 tctgcggcgg tctgcgcgtc ggcaaggatg tcgaacgcga aggtttggat ctgaatatcc
     1561 acggcgaaca cgtggaataa ggacggctat gccgtctgaa gcctgaaaat cgggtttcag
     1621 acggcatttt tcacgtttgc cgccgatggg gataaacata ctgcctgcga cgcttaacgg
     1681 ctgtctttcc actgataata tttcgagccg aggaaagaac cgagtttgcg caaaaacggt
     1741 tgcaggataa tattcggctg gcgcaactgt tcaaacggtt tcagacggca ttcgtcccct
     1801 aaaattgctt cggcaaccgc caggcctgca atgcccgtta tcgccatccc gtgcccggaa
     1861 taaccttgcg tataaaaaac attcgaggtc aaacgtccga aatgcgggac aaggttggcg
     1921 gtaatatcgc actccccgcc ccacgaatat tcgattctaa catcggcaag ctgcggaaaa
     1981 actttaagca tatcttggcg gacaagctcg gtcatacgcg caggattgtc gataaattcg
     2041 ttatccttac cgccgaaaag cagcctgccg tccgcgctga ggcggtaata atccaaaata
     2101 tggcggttgt cgcatactgc catattgttg cggataagcc cttttgtgcg cgcgcccaag
     2161 ggttcggtgg caataataaa ggtgctgacg gcaatcgcct tgcgttccaa aggccggaat
     2221 atcgggttca aaccgacata agtattgacg gcatagacca catttttaca ctcgacgctg
     2281 ccttcgggcg tgtaaaccag ccaaccgttt tgatacggtt cgatgcgcgt catcggggat
     2341 tgctcgaaaa tctgcgcgcc ggcttcggca gcggcgctgg caacacccaa cgtgtaattg
     2401 agcggatgaa gatgcccgga caagggatcg aactgtgcgc cttggtacat atcgctgtca
     2461 agctgctgtt tcaactcggc tttatcccaa agttgataat gactcgcacc gtaatgccgt
     2521 tgggcgtgtt catgccactg ctgcaactct tcccaatgct gcggacggac ggcaaccgtg
     2581 gcataaccgc gctgccaatc gcaatcgatg gcatgtttgc ggacgcgttc gtccaccagt
     2641 tcgaccgcct gcaaagactg ttgccaaaac cattgcgcct gctccaagcc gacctgtttt
     2701 tcaatttccc ccataccgca ggcgtagtcg ctgataacct gcccgccact cctgccggac
     2761 gcgccgaagc cgatacgtgc ggcttccaaa acgacggctt catgtccgtg ttccgccagc
     2821 ggcaatgcgg tacacaaacc gctcaaaccg ccgccgataa tgcaggtttc ggctttcaga
     2881 cggcattgga gtttcggata aacagtatgc ggattaaccg aactaaaata ataagaaggc
     2941 agatattctt gaaaatcagg gcgaatcatt gtgtttgctt tatcgggtat attttcggac
     3001 ggaatgatac aggctgtcgg gccatatcgt ccaaacagaa aatcggttga aaaaaaacag
     3061 gctgacccag tcatgcggtc agcctgcctt attaattaat ttgctttctc ggcagccaat
     3121 ttttcttggc ggtaggcttc tgccgcttct cggttgcgct tggttgcctg cctcatcatc
     3181 caataattga cgatgatgac caatgttccg atgatgccga tcaggatggt tgccaagaca
     3241 ttcatctgag gatcgagacc caacttgatc ttggagaaaa taacctgcgg caatgtggat
     3301 gaaccggggc cggagaggaa tgaggtaatc accaaatcat ccaaagacaa ggtaatgccg
     3361 agtaggaagc ctgaagcaat ggcaggggca atcaaaggca aagtgatgac aaaaaagatt
     3421 ttcagcggac gcgcgcccaa gtccattgcg gcttcttcga gcgactggtc aagctcgacc
     3481 agccgcgaac ggatgacaac ggtaatgtat gccatgcaca gcgtcgtatg tccgaggaag
     3541 atggtgaaaa agccgcgatc gaagtagaga tgttgcaacc attcgctgcc ctgcaaaaat
     3601 atctgcacct gaataatcag cagcagcata gacagaccgg taatcacgtc gggcatgacc
     3661 atgggcgcgg aaatcatacc ggcaaacagc gtgctgccgc gaaagcgttt gatacgcgcc
     3721 atcgcatagc ccgccagcgt gcccaaaacg acggcggcaa gcgaagacac aacggcaatc
     3781 cgcagcgaca gccaagcggc ttccaaaatg gtgtcgtttt ccagcaatgc gccgtaccac
     3841 ttggtcgaaa agccgcccca aacggtaaca agtttggatt cgttgaacga atagatgacc
     3901 aaaacaacca gcgggatata cagaaacgcc agcgatagtg ccaacatcag tttcaagaac
     3961 caagataatt tggatttctg cattatttgg ctccttcttc caattcgcgg ttttcatagt
     4021 gctggaacag ggcaatcggt acaaccagta gcgcgaccat cacgacggcg acggcagaag
     4081 ccaacggcca gttgttttga tcaaagaatg cctgccacaa tactttaccg atcatcaggt
     4141 tttccgaacc gccgaccagc tcgggaatga cgaactcacc gacagcgggg acgaaaacca
     4201 gcatcgaacc tgcaataatg ccggttttcg acaaaggcag ggtaatcgtc aagaacgatt
     4261 tgaccggtcc cgcgcccaag tcggaagccg cttcgagcag gcggctgtcc agtttcacca
     4321 gctgcgtgta aagcggcaga atcataaacg gcagataggc ataaaccatt accaaattta
     4381 acgaaaacgc attatagaac aaatctaatg gttctctgat aatacccatc ttaatcaata
     4441 agttgtttat gatgccgtta tgcccgagca aacccatcca cgcatagaca cgcagcagga
     4501 acgatgtcca aaaaggtaac atgatggcaa gcagcaaacc attgcggacg gaaggattgg
     4561 cacgggaaat cgcataggcg gtcgggtaac cgaccaacag gcaaatcacc gtcgtcgtca
     4621 gcgcagtctt aattgaagac caataagtca tcagatagat attactgttt tcactgtcgc
     4681 cgaaggggtt aagggtatgc caaaaatttt ggaaaatatc ggcatagttt tgatagctga
     4741 cggcgatgtt caggcgaccc aagtcctcat ctatcgtcgt taaaggagta aacggtggaa
     4801 tggcgatttc ttgttcggca gagctgattt tcagcacgat ggcgaacgga atcagaaaca
     4861 gcaccaaaag ccaaatatac ggtacggcaa tcaccgcacg ctgccccgga cggcggaaca
     4921 gtttgttttt cagtttatta aggttcatct cactcccctt aaatcaacgg aacaacggag
     4981 tcggttggtt ttccggccag ctgatataga cggtttcatc ccaagtcggc ggcgtgatgt
     5041 tacgcacata ccagtaaggt gcaggaactt ggcttttgac gacgcgcccg ttggcgagtt
     5101 tgatatggta tatggcgaag ctgcccaaat aggcaatttc ttttaccgtg cctttcgccc
     5161 agttgtagtc gcccaaatat tcgggttttt ctttatataa atcaatatcc tctggtcgaa
     5221 tactaaccca aagatcctgc tcgctcgggc cacccaaacc atggtcgata cggacgtggt
     5281 tttccaaacc ttcgcattcg ataacggcat agtcggcatg attttcaatc accacaccgt
     5341 caaagatgtt ggtttcgccg ataaactcgg cagtgaagcg gctgttggga tagtcgtaca
     5401 cgtcgctggg cgtgccgacc tgctgcaact gaccgtcgga cataatggcg atgcgggtcg
     5461 ccatcgtcat cgcctcttct tggtcgtgcg taaccataat gcaggttacg ccgacttgtt
     5521 ccagcgtatt gaccaactcg agctgggttt gctggcgcaa tttcttatcc aacgccccca
     5581 aaggttcgtc aagcaacagg attttcggac gttttgccaa actgcgcgcc aaggcgatac
     5641 gttgctgctg accaccggac aattggtgcg gtttgcgttt ggcaaatttg gtcatctgaa
     5701 ccagacgcag catttcttcg acgcgcgcat cgatttcgcc tttaggcatt ttgtcctgtt
     5761 tcagaccgaa ggcaatgttt tgctccaccg tcatatgcgg aaaaagcgcg taactttgga
     5821 acatcatatt gatggggcgg tcatagggtg caagtttggt aatatcttga ccatcaagga
     5881 tgatttttcc ctgattggga ctttccatac ccgccagcat acgcagcagc gtagattttc
     5941 cgctgccgga actgcccaaa agggcgaaga tttcgttttg ataaatgttc aagtcgatgt
     6001 tatcgacagc gtaattgtca ccaaactttt tcaccaaacc ttggatttgc agataaggtt
     6061 tggctgaaga cgctgtggtt gcggtcataa tggcaatact ccaataaaaa gacgagtacc
     6121 ggcaaaacgg attttcgaat gggtgataaa aagctgtttg attgctggcg ggagtcgcaa
     6181 cgtctgatgc cgtctgaaac tcttgtgaag cgcacgggca gcatgaaata aaaaggggtt
     6241 ctaaagaacc ccgcattata ttagtttata aggtttttag ggtaataaat atgggtttga
     6301 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     6361 cgcaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     6421 cggcgcaaat aggccaacag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     6481 ctgtggtggt ctttggggtt ggctttgaag tggggggtca gatcgttgat gcggaaagtc
     6541 aacagggcaa cttgtacttc agaagagccg gtgtcgcctt ctttacgttg gaaatcttta
     6601 acgatttgtg ctttttgttc tacggtcagt gccataatgg aaaaactccg aaaaataaaa
     6661 agaaccccaa atgggattcg gacaagtttg ccaagcctcg acaacggcaa actccctatt
     6721 cctgaaacag gaacagcgca ttatcacaca atttttgctg ttctgcacga tattttgcga
     6781 tgtcggcgaa atgccgtctg aaaacggaga catcttccgg tttcagacgg catttgacat
     6841 cagaccacaa tttcttcctt ctgtttcgct tccttggcga tattgtcgcg gctcaaaccg
     6901 aacatcagca agagcgggct ggcaaccaat acggaagagt agatgccgaa cacgataccg
     6961 atggtcagtg ccatagaaaa gccgtgcaag gctgcaccgc cgaacaccag catagaaaca
     7021 accatcgcct cggtcgaacc gtgggtaatg atggtgcggc tcatcgttgc ggtaatcgcg
     7081 ttgtcaatga cttccggcac ggtatgtccg cgcatcgccg gcttgcggaa attttcacgg
     7141 atacggtcga agacgacgac ggattcgttc acagaatagc ccaatacggc aaggatacct
     7201 gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa agcagccgag aataatcacg
     7261 atgtcgtgca tattggcgat aatggcagat acggcaaaac gccattcgaa acgcatcgac
     7321 aggtaaataa tgatgccgat aacgacaaaa cctaaagcca tcaatccatt atttaccaat
     7381 tcctcaccga cttgcgggcc gataaattcg acttggcgca aagtaacgtc gggactgtct
     7441 tttttcagca aatccataac ctgattggac aactgtgcgg aagtaacacc ttctttgttc
     7501 ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct gaacctgtac atcacccatt
     7561 ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg caccctgctg atattggact
     7621 tccattaccg taccgccggt aaattcgaca gagaaattca ggcctctggc aaccaaaaag
     7681 aatacggcgg caataaaggt aaccaacgaa atgaaggttg tcagtttgcc gtagctcata
     7741 aacggaatat cgcgtttgat tttaaagagt tccatagctt actccttacc tgccgcagtt
     7801 tcggcttcag gtttccatac cgcaccgatg gaaatgttct gcaatttgcg gcggcggccg
     7861 taccacagat tgaccaacgc acggaatacg acgacggacg aatacatcga agtcagaata
     7921 cccaagcagt gtacgacggc aaaacctctt accggtccgg aaccgaatac caaaagcgcg
     7981 ataccggcaa tcagcgaagt caggttcgaa tcaacaatgg tcgcccatgc gtgttggaaa
     8041 ccgagattga ttgcctgctg cggcggcacg ccggcacgca attcctctcg gatacgttcg
     8101 ttaatcaaga cgttggagtc gattgccata cccaaagtca acgccagcgc ggccataccc
     8161 ggtaacgtca acgttgcctg catggcagac aaaataccga ttagaaacag tatgttggca
     8221 ctcaatgcaa tagtagaaaa gaaacccatc aggcggtaat aaaccaccat gaatgcagca
     8281 acgatggcaa aaccccataa agtcgaatgg aagccttttt cgatgttctc cttacccaaa
     8341 gacggaccga tggtacgttc ttcgacaatc tgcatcggtg cggcaagaga accggcacgc
     8401 aacagcaaag acgtatcgtt ggcttcggct gtcgtcatgc ttccggaaat ttccacgcgt
     8461 ccgccggtaa tggcagtacg gataaccggt gcggttacaa cctcggattt tccttggtcg
     8521 atcaaaacca tcgccatgcg tttgccgaca tttgcggcag tcagttcgcc gaaaatgctg
     8581 ccgcccgcgc tgtccaagct cagactgacg gcaggtgcgc ccatttggtc gaaactcggt
     8641 tgcgcatcgt tgatgttgtc gccggtcagc tcgacctgtt tgctgatcag cagagtttcg
     8701 ggatgctccc cgccgcttga aagcagctca taaccgctcg gcacgttgcc ttccaatgcc
     8761 tcgcgcaact tggcaggatc gtcctccacc atgcggactt ccaaagtcgc ggtacggccg
     8821 atgatgtctt ttgccttggc agtatcttgc acgccgggaa gctgtacgac aatgcggtcc
     8881 aaacctgact gttggataat cggctctgcc acacccaatt cgtttacacg gttatgcagt
     8941 gtagtgatgt tttgcttcac cgcatctgat cgtactttat tcaccgcctc ttccgaaagc
     9001 gccaatacaa tattgctgcc ttctgaattt aacgttgcct caggaaacag cttgagtaac
     9061 tgaggcagtg ccttttgcac atcgccggca tcctgcaaag ggacggtcag gctgttttca
     9121 gcctgacgca ccgtgccgct gcggattttt tcacggcgca gttcgcggcg gatgtcgccc
     9181 gaataacgct caaacgtttt ctgcatcgcg gctttcatat cgacctgcat ggtgaaatgc
     9241 acgccgccgc gcaggtccaa acccaaaaac atcggattgg ctttgatttt cgccatccat
     9301 tcggggctgt ccgccaacag gttgagcgcg gtaatatacc cttcgcccaa agtgttttcg
     9361 ataacgtcgc gcgctttaag ctgcgtttct gtgtctttga aacgcacttt cagtgaatta
     9421 tccacaacaa acatcccgtc ggtctgaatg cccgcgtttt tcagcgcagc atccactttg
     9481 gattgagtct gttcgttgat gatgatggct tgtcggttgg tcgatacctg cacggcgggc
     9541 gtttcgccga ataggttggg cagcgaatac actgcggcaa ccgcaatcgt gaacacaatc
     9601 agcagatatt tccataaagg ataacggttc atcattgttc cttaatggtt ggaaccccac
     9661 cctttcggtg gtgtcggaat cgggctattt cagaagaggc aaaaacccct ccctgccagg
     9721 caagaccgaa aggcagcatc ctgaatatgc cgccctgcgt gtcggaacat ggtcaagcct
     9781 tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc ccgatgcggc ttgtcggcac
     9841 aaatcaatcg acttttgcgg caatcgcatt gcgttccact tcgacctcga tttttgtacc
     9901 ctgtccgata tccacggtaa aaaactgttc gccgactctg gttaccttac ccttgaaacc
     9961 tgccgccaag accactttgt cgccggcttt caaggcggca agcattgcct gatgcgcttt
    10021 gaatttcttt tgttgggggc gcatgatcag gaagtagaac accaccataa tcaacaccaa
    10081 aggagcaaat tgtgcaacag cttgattcat aatttatccg ttctttctaa atatgattga
    10141 aaatcgagcg gggtatataa tagcataaga ccgtaaacaa tatatcgggt ttgccgccca
    10201 tacccaaccg tatgccgtct gcaccccctt tcccgaaccc atgccgctga cccgcctgtt
    10261 cctgaaactc tacgccctgt tgcgcctttt tttgggcaaa aacgcccaga ccgcctggat
    10321 tttccacccc gcctgcaccg ggcacgaccc cggggcaaac catcccgatt cgcccgaccg
    10381 catcctctgc atcgagcagg cattgcgccg cgccggtatt tggcagcacc tccaaaccgt
    10441 agaggcggaa gaaatcagcg atacgcgcct tgcacttgtc cgctcgagca aatatctgaa
    10501 ccgtttggaa tcttgcctac ccgaagacgg caagatttgc cgtttggacg acgacaccgt
    10561 tatcagcaaa aactccctgt ccgccgcccg tttttccgcc ggttcggcag ttcaggcagt
    10621 cgatatggtc atgaaacgca aagcgtggca cgccttttgc gccgcccgcc cgccggggca
    10681 tcacgcaaaa agcggcaaag ccggcggatt ctgcctgctg aacaacgttg ccgccggcgt
    10741 catgcatgcc attgccgaat accgcctgaa acgcattgcc gtcatcgatt tcgatgtcca
    10801 ctacggcgac ggtacggcag aaatattcaa agacgatccg cgcatcctgt ttttcaacct
    10861 gtttgaaacc gaccttttcc ccttccccga aaacaacggt atgcccgacg gcggcaatat
    10921 ggtgcacctg cccttgccgt cgggaacggg cagccgcaca ttccgcgaag ccgtccgcag
    10981 acagtggctg ccccggcttg ccgcattcaa acccgaactg gtgctgctgt cggcaggatt
    11041 cgacgcacac cgtctggacg aatcgggcag gctcaacctg cacgaggcgg attttgcctg
    11101 gctgacacac aaaatcattc agacggcatc cagctgcccc ggcaaaatcg tatccgtact
    11161 cgaaggcggc tacacccttg aacctctggc acaatctgct gccgagcata tccgcgtact
    11221 ggcggggctg ggcaaatccg atgccgcaac cgcctatcag aaaacactga acccaacaaa
    11281 aaaacggttt gccaaaccga aaaccggaca ggtgcgccaa cctacccaat ccgaccgtta
    11341 cgatataaat gccgtctgaa gccgattcgg ttttcagacg gcatcattat ttgacctgac
    11401 gcaaacagaa agccttccgc gtgcttaaac acatctcttg agacctttgc aaaaatccc
//
LOCUS       DAWTHD010000075        11451 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWTHD010000075 DAWTHD010000000
VERSION     DAWTHD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.075"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..2389
                     /locus_tag="WM704_001535"
     CDS             <1..2389
                     /locus_tag="WM704_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFA9340927.1"
                     /translation="FTEKILQGDTVIPIRKVETFSQYSDGIEIKFTSIQKLYNDIHTE
                     RENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNDRTSDAEIERKGWE
                     HMVLELLLNKNGNPGQNVLLEFTATLPENTEVQQKYADKIITKFGLKEFLQKGYTKEI
                     NLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTIDESKADYLAFLN
                     WVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENKFEFVHLANWIKQN
                     YQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTVDRLTEGWDVLNLF
                     DIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEGKQPNKRKFDNDMQ
                     HELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLATFKLKSEFADNKDF
                     RELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQFTADENDETARQI
                     GTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLDIQNRNELQNKLLK
                     DWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGTKEFTPKKLWEIFG
                     TPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARLGDLKSQYDVHLIR
                     NEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFIEPKGGHLVENDSW
                     KEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTELFPLGAASLEK"
     gene            complement(2538..3710)
                     /locus_tag="WM704_001536"
     CDS             complement(2538..3710)
                     /locus_tag="WM704_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFA9340928.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(3831..4061)
                     /locus_tag="WM704_001537"
     CDS             complement(3831..4061)
                     /locus_tag="WM704_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340929.1"
                     /translation="MVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSLILLY
                     LFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            4060..4506
                     /gene="iscR"
                     /locus_tag="WM704_001538"
     CDS             4060..4506
                     /gene="iscR"
                     /locus_tag="WM704_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFA9340930.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            4535..5749
                     /locus_tag="WM704_001539"
     CDS             4535..5749
                     /locus_tag="WM704_001539"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFA9340931.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            5734..6045
                     /locus_tag="WM704_001540"
     CDS             5734..6045
                     /locus_tag="WM704_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340932.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            6378..6845
                     /locus_tag="WM704_001541"
     CDS             6378..6845
                     /locus_tag="WM704_001541"
                     /inference="COORDINATES: protein motif:HMM:NF014691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9340933.1"
                     /translation="MAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFANFAFIENES
                     RKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLFMTQLAIKNG
                     FQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQTTIF"
     gene            6858..7244
                     /gene="iscU"
                     /locus_tag="WM704_001542"
     CDS             6858..7244
                     /gene="iscU"
                     /locus_tag="WM704_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFA9340934.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            7331..7651
                     /gene="iscA"
                     /locus_tag="WM704_001543"
     CDS             7331..7651
                     /gene="iscA"
                     /locus_tag="WM704_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFA9340935.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(7611..7832)
                     /locus_tag="WM704_001544"
     CDS             complement(7611..7832)
                     /locus_tag="WM704_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFA9340936.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            7914..8414
                     /gene="hscB"
                     /locus_tag="WM704_001545"
     CDS             7914..8414
                     /gene="hscB"
                     /locus_tag="WM704_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFA9340937.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            8512..11262
                     /gene="gyrA"
                     /locus_tag="WM704_001546"
     CDS             8512..11262
                     /gene="gyrA"
                     /locus_tag="WM704_001546"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFA9340938.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDFAVYATIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT         3413 a         2964 c         2534 g         2540 t
ORIGIN      
        1 atttaccgag aaaattttgc aaggtgatac ggtaattccc attcgcaaag tggagacatt
       61 tagccaatat tcagacggca ttgaaattaa atttaccagc attcaaaaac tatataacga
      121 tattcacacc gagcgggaaa atcaaaccac attggcggat ttgcacgaat taaatcttgt
      181 gatgctgggc gatgaagcac accatttaaa cgcgcaaacc aaaggcaaaa aacaaggcga
      241 attagattta gaaaaggaaa tgaacgaccg taccagcgat gccgaaattg aacgcaaggg
      301 ttgggagcat atggttttgg agttgttact caataaaaat ggcaatcccg gccaaaatgt
      361 gctgttggaa tttaccgcca cgctgcctga aaatactgaa gtgcaacaaa aatatgctga
      421 taaaatcatc acaaaatttg gtttaaaaga atttttgcaa aaaggttata ccaaagaaat
      481 caatttggta tccggtacgc tgaataagaa agagcgggtg ttgcacgctt tattgtttgc
      541 ctggtaccgc catcaaatcg cgttgaaata cggcattgcc aatttcaagc ctgtgatgtt
      601 gtttagaagt aagacgattg atgaatcaaa agcggattat ttggcatttt taaattgggt
      661 agaaaatgtg caagcggatg atttttcgtt tttaactaca ttttcaacaa gcttaaacga
      721 tagcaataac gccaacgaac aaggcaaaac ccgtaccgaa caagccctga aatttatgca
      781 ggaaaataaa tttgaatttg tgcatttggc aaattggatt aaacaaaatt atcaaaaaca
      841 caatgtcatt attaccaact ccgaaaccaa caaaaacaaa actgaaaaaa ccgacagcga
      901 aaccgaaaaa ttgctgaaca atctggaagc ggctgataat ccgatccgtg ccatttttac
      961 ggtggataga ttaaccgaag gttgggacgt tttaaatttg tttgatattg tgcgtttgta
     1021 tgaagggcaa aacggtggcg gttcgaataa aaaatcaggc aaaacggctg ccgccaccgt
     1081 atcggaaaag cagttgattg gtcgcggcgt gcgttatttt ccatttgcgt ttgaaggtaa
     1141 acagccgaat aaacgcaaat ttgacaacga tatgcaacat gaattgcgta tcttggaaga
     1201 attgttttat tacacgcacg atgagcaatc ccgctatatt acagaactga aaaacgagtt
     1261 acgaaaagac ggttatttgc ctgaaaaaga cgatgataag gtattggcaa catttaagct
     1321 caaatctgaa tttgcagata ataaggattt tagagagttg ttaatttggg cgaataaaaa
     1381 aatccccaac ccaaacgcca aaagcaataa tgcagacagc ctgcaagcca atccgcaaac
     1441 gctttcattc caagttcacg gtaatcaact gttgcaggaa acgcaattta cagccgatga
     1501 aaatgatgaa acagcccgac aaatcggcac acaaaataat tttactcaaa ccataaaaat
     1561 gagtgaaatg gagcggcata ttttcaataa agcattacat atcaaaggga aaaatagtca
     1621 atctttattc cattttgacc gcctgcaaag caaactcgac attcaaaatc gcaatgaatt
     1681 gcaaaataaa ttgttgaaag actggcagat tgaatttttg ggattagagc aagataaaca
     1741 ggttcgcccg gatgataaac ttgcaggctg cctgaaaatc ttggaaatgg ttgaaaaaca
     1801 tctgaatgaa agtgatatac cgtttatcgg tacaaaagaa tttacaccta aaaaattgtg
     1861 ggaaattttc ggcacgccaa aacaaaaatg ggtcaaaaaa gatgatataa aaactgctat
     1921 tgccacgcaa aatgattggt atgtgatgga taattttgcc ggaacgggtt tggaagaagc
     1981 gttaattcaa tttatttcag cgcgcttggg cgatttgaaa tctcaatatg acgttcattt
     2041 aatccgcaat gaagaagtgt ttaaattgaa taactttgcc gatggcgaag gatttatgcc
     2101 ggactttgtt ttattgctga aagataaaca aaaatcttct tccgacagcg tggacgactt
     2161 tttgcattac caaattttca ttgaaccgaa aggcgggcat ttagtggaaa atgattcgtg
     2221 gaaagaagct tttttaaaat caatcacagt ggaatatggg agggataaga tcctgcaaaa
     2281 aaatacaccg cattatcgtt tgatcggttt gccgtttttt actgatcatc agaaaaatgg
     2341 acaatttaca gagttattcc ctttaggtgc ggcatcgctt gaaaaataga gtagtgcatt
     2401 gcaggcagcc ccgttcggca aaacttcctt tacaaaaggg cgttttgtca gatatttaat
     2461 caacacatta ttaaaataca accaaatttt aatgccgtcc gaaccatgta ttcagacggc
     2521 atcgtatttt tcagtatcta aaccgtttcc ctgccccaat ccttactcct caaaatcgag
     2581 gcatcgacat cttgaatatc gcggtgtccc gtaaacgcca tcgacacatc catttccttg
     2641 tacaaaatct ccagcgcgcg cgttacgcct tcttcgccat acgcacccaa accatacagg
     2701 aacgcccgac ctatcatcgt gcctttcgca cccaaagccc acgccttcaa aatatcctgg
     2761 ccgctgcgga taccgctgtc catccaaact tcgatgtcac tgccgactgc ggaaacaata
     2821 tcgggcaagg ccttgatggc ggacacagta tcgtcgagct ggcggccgcc gtggttggaa
     2881 acgaccaatg catccgcacc gcttttcgcc gccttttccg cgtcttcggg ttccataatg
     2941 cctttgataa tcagcttgcc gccccacaaa tctttaatcc gcgccacgtc gtcccagctt
     3001 aagcgcgggt cgaactgttc cgccgtccac gaagaaagtg aagacaaatc gccgacgttt
     3061 ttggcgtgtc cgacgatatt gcggaacgtg cggcgttccg tgttcagcat tttcatgcac
     3121 cattcgggct tggtcgccag attgattaaa ttggcgatgg tcggtttcgg cggcgcggac
     3181 aggccgtttt tgatgtcttt gtggcgttga cccaaaacct gcaaatcggc agtcaatacc
     3241 aatgccgaac atttggcatc tttcgcacgc ttaatcaggt tttccataaa ttcgcggtcg
     3301 cgcatcacat aaagctgaaa ccaaaacggc gcgctggtgt tctcggcaac gtcttcaatc
     3361 gagcagatgg acatggtgga caacgtaaac ggaataccga acttctccgc cgcacgcgcc
     3421 gccaaaattt caccgtctgc gtgtgccatg ccggtaaaac ccgtcggcgc aatcgccacc
     3481 ggcattttca catccccccc gatcattttg gtttcgaggc tgcgtccctc catattgacc
     3541 aatacctttt gtcggaagcg gatatctttg aaatccgaag tattctccct ataagtcgtt
     3601 tccgtccacg aacccgaatc gatgtaatcg taaaacatac gcggcatttt gcgcttggca
     3661 acgcggcgca agtcttcgat gcaggtcatt ttgctcaaat cacgtttcat cttgtttttc
     3721 cctgtttaac agcctgatgg aatatgccgt ctgaaactgc ttgagccttc aaacggcatt
     3781 tcgggcaata cccaaataaa tcactaagat ttagataatc tatatcaata ttaaaaataa
     3841 atcaaatcgt tttgaaaccg cttgccggca aaacgcccca accgccgctc ctcaaaaaac
     3901 ataactaact gattcaaaaa accaaacaaa taaagcaaaa tcagactata ccgccattta
     3961 aacaaacaat cggcaacagc tcccccatac ttgactgaaa cactcagata ttggacaatt
     4021 ccgcccacta taaaaaagcc gacacgggca accaccacca tgagactgac caccaaaggg
     4081 cgtttcgccg ttaccgctat gctggatttg gcgatgaacg cgcaaaccgg cgccgtcaaa
     4141 ctcagtgcca tcagcgaacg ccaaaatata tccctctcct atctcgagca attgttcggc
     4201 aaactccgcc gcgccggact ggttgaaagc ctgcgcgggc ccggcggcgg ctacatcctc
     4261 gccgcccctc ccgcacaaat caacatcgcc caaatcattt ccgccgccga agaccgtctg
     4321 gacgcgaccc aatgcggcag caaagccaac tgccaccacg gcgcgccctg cctgacgcac
     4381 gacctttggg agaatttaaa caaaaccatc aacgactatc tcggcagcgt taccctgcaa
     4441 agcatcatcg aacagaaaaa caacggcgac ggcagccgcg tcgtccaatt tacacacatc
     4501 cattaaataa cacccgaaaa agaaagagca aaccatgacc gtcaaaaccc ccgtttacct
     4561 cgactacgcc gccaccacac ccgttgacaa acgcgttgcc gaaaaaatga ttccctatct
     4621 gactgaaacc ttcggcaacc ccgcctccaa cagccacgca ttcggctgga cggcagaaga
     4681 agccgtcgaa aaagcccgcg ccgacatcgc cgccctgatt aacgccgacc ccaaagaaat
     4741 cgtcttcacc agcggcgcga ccgagtccga caacctcgcc atcaaaggtg cggcaaactt
     4801 ctacaaaacc aaaggcaaac acctcatcac cgtcaaaacc gaacacaaag ccgtgctcga
     4861 cacgatgcgc gaactcgaac gccaaggctt tgaagttacc tacctcggcg tgcaggaaaa
     4921 cggtttgatt gatttggaag aactcaaagc cgccatccgc gacgacacca tcctgatttc
     4981 cataatgtgg gtgaacaacg aaatcggcgt ggtgcaaaac attcccgcca tcggcgaaat
     5041 ctgccgcgaa cgcaaaatcg ccttccacgt cgatgccgcc caagcctgcg gcaaagtgcc
     5101 tgtcgatgtc gaagccgcca aaatcgactt gctctcgatg tccgcgcaca aagtgtacgg
     5161 ccccaaaggc atcggcgcgc tgtacgtccg ccgcaaaccg cgcgtccgcc tcgaagccca
     5221 aatgcacggt ggcggtcacg agcgcggttt ccgttccggc acattgccga cccatcaaat
     5281 cgtcggcatg ggcgaggcct tccgcatcgc caaagaagaa ttggcacaag acacagcgca
     5341 ctacctgaaa ctgcgcgaca tcttcctcaa aggcatcgaa ggcatcgaag aagtctatat
     5401 caacggcgac ctcgaacacc gcgccccgaa caacctgaac gtcagcttca acttcgtcga
     5461 aggcgaaagc ctgattatgg cggtgaaaga actcgccgta tccagcggat ccgcctgcac
     5521 ctccgcctcg ctcgaaccca gctacgtcct gcgcgcgctt ggccgcaacg acgaactggc
     5581 gcactcatcc ctgcgcatca ccttcggccg catgaccacc gaagaagaag tgcaattcgc
     5641 ggcagaactg atcaaatcca aaatcggcaa actgcgcgaa ctgtcgccgc tgtgggaaat
     5701 gttcaaagac ggtattgatc tgaactcgat tgaatgggcg gcgcattaag attgatgtca
     5761 aacaaaggct gtctgagaaa gaataccatg aaagttaaaa ttattaattt gccaaatggt
     5821 tataaacgta ttatttatgg aaaatatttt gaacaatttg acttagatta tgaacaagat
     5881 ttagacgtat taaaaaaaga tattgaattt gctttatctg ttattgaata caatcgatct
     5941 atattcaaaa aattctcttc cttatttgag aataaaatca tttttgttta tcaaggagga
     6001 catcatcttg atattattga tcgtgataaa ggaagtttga aatagttaac tattgaagat
     6061 cattggacaa aagatgttga tgatatcact ggttcatgga aaatggagaa tttagaaatt
     6121 agccgacaaa ctcaatatgc aatgcaactt ctaaagattg gtaaaacaaa tattgagaaa
     6181 gaaataagaa aaattaaaca aaggttctaa aaaaatgagt aaatacggtg gtgaagacgt
     6241 aatctatatg aatgatggtg tatttagaaa taaattagcc attcaagatc cacttaaatt
     6301 taaaaaaggt cgaaagagac atttctgcta tttccgccca aaatctgctt ttgcaaccca
     6361 ttacaggcaa tttcgatttg gcgcatttac agaccattca tcgcgaactg tttggcaatg
     6421 tttatgattg ggcaggaaaa atccggaggg ttgatatttc taaaggtaat acccgatttg
     6481 ccaattttgc ttttatcgaa aacgaaagcc gtaaactttt ggagaagctg aaaaacgaaa
     6541 actatttgcg ggtgttggat aaagataaat tcgccgagcg tgcagcctat tatttggacg
     6601 agttgaacgt cctacatcct tttcgtgaag gaaacggcag aacattgcgc ctgtttatga
     6661 cacaattggc aataaaaaac ggttttcaga tacattggca aaatatctct gccgaacaaa
     6721 tgattcaagc ctgtattcag gcataccatg ccgatagcag cttattggca cgcctaatta
     6781 ttagacaatt tagaacaatc gtctttttca gacgactaaa acatcaacaa accacgatat
     6841 tttaaggaaa ccacatcatg gcatacagcg ataaagtaat cgaccactac gaaaatcccc
     6901 gcaacgtcgg cactttcgac aaaaacgacg agtccgtcgg caccggcatg gtcggcgcgc
     6961 ccgcctgcgg cgacgtgatg cgcctgcaaa tcaaagtgaa cgatgaaggc atcatcgaag
     7021 atgcgaaatt caaaacttac ggctgcggtt ccgccatcgc ttcgtccagc ctgattaccg
     7081 agtgggtcaa aggcaaaagt ctggatgacg cgctggcaat caaaaacagc gaaatcgccg
     7141 aagaactgga attgccgccg gtaaaaatcc actgctccat cttggctgaa gatgcggtaa
     7201 aagcggccgt tgccgactac cgcaaacgtc aggaaaacag ataaagccct tcagacggca
     7261 tcgtcccaca atgccgtccg aaccacccgc cgcttcggat ccgtccgggg cggtacaaca
     7321 aggaagaaat atgattaccc ttaccgagaa tgccgcaaaa cacatcaatg actatctcgc
     7381 caaacgcggc aaaggcttgg gcgtacgctt gggtgtaaaa accagcggct gctcggggat
     7441 ggcgtacaac cttgaatttg tcgatgaagc caacggcgac gacctgattt tcgaaggaca
     7501 cggcgcgcgc atttatatcg acccgaaaag cttggtttat ctggacggca cacaagtcga
     7561 ttacaccaaa gaagatttgc aggaaggttt caaatttgaa aaccccaatg tcaaagactc
     7621 ctgcggctgc ggcgagagct tccacgttta aggcataaaa acggcgggac agtatcaaaa
     7681 ccgtcccgcc gttttttcgc ttcctgcctg ttatagctgc ctttgccttt ccttttccgt
     7741 tccaccttgt gccggaacaa atcggatttt actaaggctt ttaaagcatt gtcgcgtatt
     7801 ttgcctttat tgtgctgcgc tttgccgccc atattcagtc ctttcgttta agaagccgca
     7861 gattataagg caaaaacagt tttctgccaa aatcttacat ttatcatcct actatgtccc
     7921 aatatttcac cctcttccgg attgaacccg ctttcgatat cggcaccgaa aacttggaac
     7981 aaacctaccg cgccttggcc gcccgtttcc atcccgacaa attcgcttcc gcttccgctt
     8041 tcgagcagaa acaggcggtt atgatgtctt ccaccatcaa cgatgcctac cgcaccttga
     8101 aaaatcccat cgaccgcgcc gcgtacctgc tgaaaacatc gggtatcgat gccgacgcgc
     8161 cggaacatac ctctttcgcc cccgacttcc tcatgcagca aatggaatgg cgcgaaacgc
     8221 tgatggaggc acgggcaggc aacaaccttg aatccttgaa aaatctcgat aacgaaatcc
     8281 gcgccgaaca agaaaaactg ttctgcggtc tgaaacagtc attcgcgcgc caagactgcg
     8341 acaccgccgc acaacaagtc cgccaaggca ggtttctcga caaactccgc cacgaaattt
     8401 cctccgcatt ataatccgca ccgtgtttca gacggcataa ccgccgcacc gtttcatgtc
     8461 gaaatatgct aaaataagca acaatttttt gccatacgaa acattgaaac catgaccgac
     8521 gcaaccatcc gccacgacca caaattcgcc ctcgaaaccc tgcccgtcag ccttgaagac
     8581 gaaatgcgca aaagctatct cgactacgcc atgagcgtca ttgtcgggcg cgcgctgccg
     8641 gacgttcgcg acggcctaaa gccggtgcac cggcgcgtac tgtacgcgat gcacgagctg
     8701 aaaaataact ggaatgccgc ctacaaaaaa tcggcgcgca tcgtcggcga cgtcatcggt
     8761 aaataccacc cccacggcga tttcgcagtt tacgccacca tcgtccgtat ggcgcaaaat
     8821 ttcgctatgc gttatgtgct gatagacgga cagggcaact tcggatcggt ggacgggctt
     8881 gccgccgcag ccatgcgcta taccgaaatc cgcatggcga aaatctcaca tgaaatgctg
     8941 gcagacattg aggaagaaac cgttaatttc ggcccgaact acgacggtag cgaacacgag
     9001 ccgcttgtac tgccgacccg tttccccaca ctgctcgtca acggctcgtc cggtatcgcc
     9061 gtcggtatgg cgaccaacat cccgccgcac aacctcaccg acaccatcaa cgcctgtctg
     9121 cgtcttttgg acgaacccaa aaccgaaatc gacgaactga tcgacattat ccaagccccc
     9181 gacttcccga ccggggcaac catctacggc ttgggcggcg tgcgcgaagg ctataaaaca
     9241 ggccgcggcc gcgttgttat gcgcggtaag acccatatcg aacccatagg caaaaacggc
     9301 gaacgcgaag ccatcgttat cgacgaaatc ccctatcagg tcaacaaagc caagttggtc
     9361 gagaaaatcg gcgatttggt tcgggaaaaa acactggaag gcatttccga gctccgcgac
     9421 gaatccgaca aatccggtat gcgcgtcgtt atcgagctga aacgcaacga aaatgccgaa
     9481 gtcgtcttaa accaactcta caaactgact ccgctgcaag acagtttcgg catcaatatg
     9541 gtggttttgg tcgacggaca accgcgcctg ttaaacctga aacagattct ctccgaattc
     9601 ctgcgccacc gccgcgaagt cgttacccga cgtacgcttt tccggctgaa gaaggcacgc
     9661 catgaagggc atatcgccga aggcaaagcc gtcgcactgt ccaatatcga tgaaatcatc
     9721 aagctcatca aagaatcgcc caacgcggcc gaggccaaag aaaaactgct tgcgcgccct
     9781 tggcgcagca gcctcgttga agaaatgctg acgcgttccg gtctggattt ggaaatgatg
     9841 cgtccggaag gattggctgc aaacattggt ctgaaaaaac aaggttatta cctgagcgag
     9901 attcaggcag atgctatttt acgcatgagc ctgcgaaacc tgaccggcct cgatcagaaa
     9961 gaaattatcg aaagctacaa aaacctgatg ggtaaaatca tcgactttgt ggatatcctc
    10021 tccaaacccg aacgcattac ccaaatcatc cgtgacgaac tggaagaaat caaaaccaac
    10081 tatggcgacg aacgccgcag cgaaatcaac ccgttcggcg gcgacattgc cgatgaagac
    10141 ctgattccgc aacgcgaaat ggtcgtgacc ctgacccacg gcggctatat aaaaacccag
    10201 ccgaccaccg actatcaggc tcagcgtcgc ggcgggcgcg gcaaacaggc ggctgccacc
    10261 aaagacgaag actttatcga aaccctgttt gttgccaaca cgcatgacta tttgatgtgt
    10321 tttaccaacc tcggcaagtg ccactggatt aaggtttaca aactgcccga aggcggacgc
    10381 aacagccgcg gccgtccgat taacaacgtc atccagctgg aagaaggcga aaaagtcagc
    10441 gcgattctgg cagtacgcga gtttcccgaa gaccaatacg tcttcttcgc caccgcgcag
    10501 ggaatggtga aaaaagtcca actttccgcc tttaaaaacg tccgcgccca aggcattaaa
    10561 gccatcgcac tcaaagaagg cgactacctc gtcggcgctg cgcaaacagg cggtgcggac
    10621 gacattatgt tgttctccaa cttgggcaaa gccatccgct tcaacgaata ctgggaaaaa
    10681 tccggcaacg acgaagcgga agatgccgac atcgaaaccg agatttcaga cgacctcgaa
    10741 gacgaaaccg ccgacaacga aaacaccctg ccaagcggca aaaacggcgt gcgtccgtcc
    10801 ggtcgcggca gcggcggttt gcgcggtatg cgcctgcctg ccgacggcaa aatcgtcagc
    10861 ctgattacct tcgcccctga aaccgaagaa agcggtttgc aagttttaac cgccaccgcc
    10921 aacggatacg gaaaacgcac cccgattgcc gattacagcc gcaaaaacaa aggcgggcaa
    10981 ggcagtattg ccattaacac cggcgagcgc aacggcgatt tggtcgccgc aaccttggtc
    11041 ggcgaaaccg acgatttgat gctgattacc agcggcggcg tgcttatccg taccaaagtc
    11101 gaacaaatcc gcgaaaccgg ccgcgccgca gcaggcgtga aactgattaa cttggacgaa
    11161 ggcgaaacct tggtatcgct ggaacgtgtt gccgaagacg aatccgaact ctccggcgct
    11221 tctgtaattt ccaatgtaac cgaaccggaa gccgagaact gaaaatcatc tcccgatgcc
    11281 gtctgaagat tcagacggca tttatttttc cctcatccgt catccagctt ctcacaatat
    11341 agcggattaa caaaaatcag gataaggcga cgaagccgca gacagcacaa atatagtgga
    11401 ttaaatttaa actagtacag cgttgcctcg ccttgccgta ctatagtcaa t
//
LOCUS       DAWTHD010000076        11136 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWTHD010000076 DAWTHD010000000
VERSION     DAWTHD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.076"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            170..2543
                     /locus_tag="WM704_001547"
                     /pseudo
     CDS             170..2543
                     /locus_tag="WM704_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(2752..3225)
                     /locus_tag="WM704_001548"
     CDS             complement(2752..3225)
                     /locus_tag="WM704_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFA9340939.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(3356..4711)
                     /gene="yegQ"
                     /locus_tag="WM704_001549"
     CDS             complement(3356..4711)
                     /gene="yegQ"
                     /locus_tag="WM704_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFA9340940.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            5037..5825
                     /locus_tag="WM704_001550"
     CDS             5037..5825
                     /locus_tag="WM704_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFA9340941.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(5871..6470)
                     /locus_tag="WM704_001551"
     CDS             complement(5871..6470)
                     /locus_tag="WM704_001551"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFA9340942.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            6598..7215
                     /gene="gmk"
                     /locus_tag="WM704_001552"
     CDS             6598..7215
                     /gene="gmk"
                     /locus_tag="WM704_001552"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFA9340943.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            7273..7479
                     /locus_tag="WM704_001553"
     CDS             7273..7479
                     /locus_tag="WM704_001553"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFA9340944.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            7572..9728
                     /locus_tag="WM704_001554"
     CDS             7572..9728
                     /locus_tag="WM704_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFA9340945.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDTQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(9795..10979)
                     /gene="coaBC"
                     /locus_tag="WM704_001555"
     CDS             complement(9795..10979)
                     /gene="coaBC"
                     /locus_tag="WM704_001555"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFA9340946.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         2695 a         2687 c         3026 g         2728 t
ORIGIN      
        1 aaataatgaa acaaacaagg cctcggcata gatgtttgca gagaccttta attacacgac
       61 acagatttat ttgtacgggt tgctgtctta ttaaatatta atgattatca gaaaatttat
      121 tatgaattaa ctgatggatg aacaatccat tacatcttga gttgataata tgaaaccatt
      181 acacatgctt cctattgccg cgctggtcgg cagtattttc ggcaatccgg tcttggcagc
      241 ggatgaagct gcaaccgaaa ccacacccgt taaagcagag ataaaagaag tgcgcgttaa
      301 agaccagctt aatgcgcctg caaccgtgga acgtgtcaac ctcggccgca ttcaacagga
      361 aatgatacgc gacaacaaag acttggtgcg ttactccacc gacgtcggct tgagcgatag
      421 cggccgccat caaaaaggct ttgctgtgcg cggcgtggaa ggcaaccgtg tcggtgtcag
      481 cattgacggc gtgagcctgc ctgattcgga agaaaactca ctgtatgcac gttatggcaa
      541 cttcaacagc tcgcgcctgt ctatcgaccc cgaactcgtg cgcaacatcg aaatcgcgaa
      601 gggcgctgac tctttcaata ccggtagcgg cgcattgggt ggcggcgtga attaccaaac
      661 cctgcaaggc catgatttgc tgttggacga caggcaattc ggcgtgatga tgaaaaacgg
      721 ttacagcagc cgcaaccgcg aatggacaaa tacactcggt ttcggtgtga gcaacgaccg
      781 cgtggatgcc gctttgctgt attcgcaacg tcgcggtcat gagaccgaaa gcgcgggcga
      841 gcgtggctat ccggtagagg gtgctggcag cggagcgaat atccgtggtt cggcacgcgg
      901 tatccctgat ccgtccaaac acaaatacca caacttcttg ggtaagattg cttatcaaat
      961 caacgacaag caccgcatcg gcgcatcgtt taacggccag caggggcata attacacgat
     1021 ttgaagagtc ttataacctg accgcttctt cctggcgcga agccgatgac gtaaacagac
     1081 ggcgcaatgc caacctcttt tacgaatgga cgcctgattc aaattggttg tcgtctttga
     1141 aggcggactt cgattatcag aaaaccaaag tggcggcggt taacaacaaa ggctcgttcc
     1201 cgatggatta ttccacctgg acgcgcaact ataaatcaga aggatttgga cgaaatatac
     1261 aaccgcagca tggacacccg attcaaacgt tttactttgc gtatggacag ccaaccgttg
     1321 caactgggcg gccaacatcg cttgtcgttt aaaactttcg ccagtcggcg tgagtttgaa
     1381 aacttaaacc gcgacgatta ttacttcagc ggacagatat cccgtactac cagctcgatt
     1441 caacaccctg tgaaaaccac taattatggt ttctcactgt ctgatcaaat ccaatggaac
     1501 gacgtgttca gcagccgtgc agatatccgt tacgatcata ccaaaatgac gcctcaggaa
     1561 ttgaatgccg agtgtcatgc ttgtgacaaa acaccgcctg cagccaatac ttataaaggc
     1621 tggagcggat ttgtcggttt ggcggcgcaa ctgaatcagg cttggcatgt cggttacgac
     1681 attacttccg gctaccgtgt ccccaatgcg tccgaagtgt atttcactta caaccacggt
     1741 tcgggtaatt ggctgcccaa tcccaacctg aaagccgagc gcagcaccac ccacaccctg
     1801 tctctgcaag gccgcagcga aaaaggtact ttggatgcca acctgtatca aaacaattac
     1861 cgcaacttct tgtctgaaga gcagaagctg accaccagcg gcgatgtcgg ctgtactcag
     1921 atgaattact actacggtat gtgtagcaat ccttattccg aaaaaccgga atggcagatg
     1981 caaaatatcg ataaggcccg aatccgtggt cttgagctga caggccgtct gaatgtggac
     2041 aaaatagcgt cttttgttcc tgagggctgg aaattgttcg gctcgctggg ttatgcgaaa
     2101 agcaaactgt cgggcgacaa cagcctgctg tccacacagc cgccgaaagt gattgccggt
     2161 gtcgactacg aagcccgagc gaaaaatggg gtgtgttctc ccgcctgact tatctgggtg
     2221 cgaaaaaggc caaagacgcg caatacaccg tttatgaaaa caagggccgg ggtacgcctt
     2281 tgcagaaaaa ggtaaaagat tacccgtggc tgaacaagtc ggcttatgtg tttgatatgt
     2341 acggcttcta caaaccggct aaaaacctga ctttgcgtgc aggcgtatat aatgtgttca
     2401 accgcaaata caccacttgg gattccctgc gcggtttgta tagctacagc accaccaacg
     2461 cggtcgaccg agatggcaaa ggcttagacc gctaccgcgc ctcaggccgt aattacgccg
     2521 tatcgctgga atggaagttt taatctagta ttattggatt aattgcctcg ttggggatta
     2581 aagctgtccg aattgttttc aggaactcat tcggacggct ttttaccgta aatctgtgtg
     2641 atggggtttt atattgatac agtatccgaa ctcagggcag ggagtaatgt tgcaggatta
     2701 aaaatgccgt ctgaaaaatg tttcagacgg cattttttga ttgccgatat gttaacggac
     2761 gacattggcg cgcatgaccg cgcggacgtg gaacgaacct gcctgacagt cgtattgtcc
     2821 gccttgcagc gattgcttgc caaagtagct gtggaagccg gtaacactgc gcccgccgag
     2881 tttgccggcg gtctgctgga atttgcgggc ggcattgatt agggcgtgtt cgcaggcctc
     2941 ggagtcggat ttgccaacgc cgtttgccgc ttgccgtgta actagattcc tgccgtttgc
     3001 aaccggtacg ccgccgccga agctgacggg aatccctgat tccaatacgc cggcggcggt
     3061 ggctgcattt attgccgcct gaccgttgct acaacggtaa atgatgtttt tgccgcctcc
     3121 tgcggtgtct ccgcggataa ctttgcatga ttctccggcc ggtgtcccgg cggatgccgt
     3181 gtgcgcgttt ttaagcgcgc ggccggattg gcaggctgcc agcatcaggg cggcggcgga
     3241 gagtatgagg attttttatg tcggatgtcc ttgtttgctg tgggaaatca ttgccattgt
     3301 acagtaagat tcaacaaaat gccgtctgaa aacggggttc ggacggcatt gtggctcagg
     3361 ggttcaacac gcgtgcgatg agggcttttt ctttgccctg catattgggg attttgacct
     3421 gaatgccgtt gcccggggca acatcgacag gttggccttt gcgggtcatt tgttccaatt
     3481 tgatggtttg gttgccgctc gggtggatga tttcgagcga atcgccgacg gcgaagcggt
     3541 ttttaacttc gatggtcgcc cagccatttt cgtcgatttc ggtaacgtgt ccgacgtatt
     3601 ggctttgttt ggcggtggaa tggccgctga ggtagttttg ataatcctga gtttggtggc
     3661 gttcgaggaa gccgctggtg tagcctcggt tggcaaggcc ttcgagttcg ctcaacaggc
     3721 tgtaatcaaa cggacggcct gcgacggcat cgtgaatcgc tttgcggtag gactgggcga
     3781 cgcgggcgac gtagtagaga gacttggtac ggccttcgac tttgaggctg tccacaccga
     3841 ttttggcgag tttttcgacg acttcgatac cgcgaagatc tttggagttc atgatgtagg
     3901 tgccgtgttc gtcttccata atcggcatca tctcgcccgg gcggttggat tcttcaatca
     3961 ggaaaacttt gtcggcgtag ggatggcgtt tttgaccgtt gatgccttca aagttttggt
     4021 tggcttcttc ttgggctttt tcaaagttga aaccttgcag aagctgggca tcgcctgcct
     4081 cgctttccgt ggcgttgtgg actttgtagt cccaacggca ggagttggtg caggtgcctt
     4141 ggttgggatc gcggtggttg aaatagcccg acaacaggca gcggcctgag taggcgatgc
     4201 acaatgcgcc gtggatgaag acttcgagtt cgatgtcggg gcattcttgg cggatttcgg
     4261 caatttcttc catgcccaat tcgcgtgaca ggatgatgcg ttcgacacca atgttctgcc
     4321 aaaatttcac gccccagtag ttggtggtgt tcgcctgtac ggacagatgg atcggcattt
     4381 ccggccattt ttcgcgcacg gtcataatca aacccggatc cgccataatc agcgcgtcgg
     4441 gtttcatggc aatcagcggc tccatgtcgg caacgaaggt tttgagtttg gaattgtgcg
     4501 gcagggtgtt gacggtcaaa aagaattttt tgttgcgttc gtgcgcttct ttaatgcctt
     4561 gctcgaggac gtcgagtttg gcaaattcgt tgttgcgggc gcgcagggag taacgcgggc
     4621 tgccggcgta aacggcatct gcgccgtaat cgtaggcggc gcgcattctt tccaatccgc
     4681 cggcgggcaa taagagttcg ggtgctttca tcgtgtttcc ttttcggttg aaaccccgcc
     4741 ctttagggcg gcaggatcag actttgtttg ggaggggcgt aaccccttcc gaatcaggac
     4801 gacataatag ggcggtgctt tatgtgtcgt cctgtgtgtt gaagcatgat ttgtatgctt
     4861 tgtaaaaatg tttcagacgg cattgctgca aatgccgtct gaagggtgtt tgaagatggg
     4921 cggattatcc gccttttcgt gttcgcggtc aattttgccg ggaaatttat aaggatttat
     4981 caagtatttg ccttcttggg atacaatgcg gcagtttgag tgcgagagag aatatgatga
     5041 atcccaaaat cgtgtttttc gacattgacg atacgctgta ccgcaaatat acggatacct
     5101 tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg caaaggtata ttgacggcgt
     5161 tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt cagggacatg atggcggaaa
     5221 cgggtatgga tgccgtggta acgataaacg gacagtttgc gctgctgcac ggtaaaactg
     5281 tgcgcgaagt accgatggat accggtttga tgggcagggt ttgtgcgcat ttggatggct
     5341 tgggcatgga ttatgcgttt gtcggcggag aggggatcgc cgtgtccgcg ctgtcggaat
     5401 gcgtgtgccg cgccttgcag catatcgcca gcgatttttt tgccgataag gattattttt
     5461 caagcaaacc ggtgtatcag atgctggtgt ttgcggagga aaacgaaatg ccgctttggt
     5521 cggatattgt ggaacgggaa ggcttgaaaa cggtgcgctg gcacgaggaa gcggtcgatc
     5581 tgctgcctgc gggaatgtcg aaaacagacg gcatcagaag cgtggttgaa gcattgggtt
     5641 tggaaatggc agacgtgatg gcgttcggcg acggtttgaa cgatgtggaa atgctgtctg
     5701 aagtcgggtt cggcgtggca atgggtaacg gggaacaggc agcgaaagaa gcggcgaaat
     5761 atgtttgccc cggcgttgat gaagacggcg tgttgagggg cttgcaagat ttgggtgtga
     5821 tttgaacgta taataacaac cctgccggtt tcagacggca gggtcggttt tcagcctttc
     5881 atgcagcctt cgttttgaag cagggtaaat aagggcgcgc cacttgcgcg gatattcttg
     5941 ccgccttgaa ggtcggtaaa ttccaaaatg gcggcggctt cgacaatttc cccgccgagt
     6001 ttgcggatca gttccagccc ggcaagcatt gtgccgcccg tggcaaccaa atcatcgacc
     6061 agcaggacgc gcgaaccggg tttgacggca tcggtgtgga tttccaccgc agcttccccg
     6121 tattcgagcg cgtagctttg cgatacggtt tcaaaaggca gcttgccttt tttgcggatg
     6181 gggacgaagc cgacgttgag ctggtaggcg agtgccgcgc cgataatgaa gccgcgcgcg
     6241 tccaagccgg caacgatgtc gattttctga tccatatagc ggtaaaccag caaatcgacc
     6301 aaaaggcgga agtattccgc actttgcagg acgggcgtga tgtcgtggaa taagatgcct
     6361 ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa gcgcgccgac actcatagct
     6421 tcgggatgaa ccagcattgc gtgttccaag tttgatgttt ttaacgccat attgtagccg
     6481 tcagcctgcc gcttgccaaa tcaatcaaaa caatagcggc aaaatatggg catcggcaaa
     6541 tatgaaatag ggtgtccgtt gtgatacaat cggttggaaa atatacaggt aaaaaatatg
     6601 tccgcttatc gaaaaggcaa catctttatc atttcggccg cttccggcac gggcaaaacc
     6661 acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc gcgtttccgt gtcgcatacg
     6721 acgcgcccgc cgcgtgaagg cgaagcaaac ggcgtacatt atcactttgt ttccaaagaa
     6781 gagtttgaat cgcttatcgc gcaggaggct tttttggaat acgcagacgt attcggcaac
     6841 tattacggca caagcacgga gggcgtgaat gcgttggcgg cggcaggcta tgacgtgatt
     6901 ttggaaatcg acgttcaggg cgcggcgcag gttcgcaacg cgctgccgga agctgtcggt
     6961 atctttatcc tgccgccttc tttcgacgtg cttgccgcgc gcctcaaagg gcgcgggacg
     7021 gacagtcggg aagttatcca aaggaggctg tcgaaggcaa ggcatgaaat cgaacagtcc
     7081 gtattgttcg actttgtcgt ggtcaatgac gacctggaga aggcggaggg ggatttgctt
     7141 catattgtga atgcctgccg tctgaaaagg tcgcggcaac tgggatttat tgcagatttg
     7201 ttggaaaatt cctaaaaacc gcgaaaatat ccggtttccc aatttaaata tttttgaaag
     7261 aaagcaaata atatggcacg tattaccacc gaagactgta ccggaaaaat ttccaaccat
     7321 tttgacctga cattggtagc ggcccgtcgg gcccgccagc ttgaaaatgg caatacgccg
     7381 cttgtggacg atgtccgcaa taacaaaccg accgttaccg ccttaaggga aatcgcagcc
     7441 gggcatatcg gtacggaact gttgacgcgc aataaataaa ttctgccgga aacgcacgcc
     7501 ggcacactct gccgccgtgc agtccgacgg tttgaaatga aaaaacacat acatcccgaa
     7561 agccgtctga aatgcccgcc ccccaacctt ccgcccctta cgaccccctg accgccgaag
     7621 cgcgtaccct gcttttccat accgcctcct acctcaagcc cgaggaacag gcggagcttg
     7681 aaaaagctgt cgcctatgcg tttcgcgccc acgacgggca aacccgcaaa agcggggatc
     7741 cctacatcac gcatccgatt gccgttgcga cgcagctcgc cctttggcat atggacacac
     7801 agggtctttg tgcaggcgtg atgcacgacg tattggaaga tacgggcgtg acaaaagggg
     7861 aaatggcggc ggtgttcggc aatactgttg ccgagatggt ggacgggctg tccaaactcg
     7921 aaaaactcaa atttgaagat catgcggaac atcaggcgga gagtttccgc aaactgattt
     7981 tggcgatgac caaagatgtg cgcgtcatcg ttgtcaaact tgccgaccgc ctgcacaata
     8041 tgcggacgct cggttcgatg cgcccggaca aacgccgccg gattgcaagg gaaacccttg
     8101 aaatctacgc acagattgcc aaccgcatag gtctgaataa tgcgtatcgg gagcttcagg
     8161 atttatcgtt ccaaaacctg catcccaacc gctacgagac tttaaaaaaa gcgatggaca
     8221 agagccggaa gaacaggcag gacgttgtcg gcaaagtttt gcgcgcgttc ggccagcggc
     8281 tggtaggcgc gaatatagag gccaaaatca aaggcaggga aaaaaacctg tacggcatcc
     8341 atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt tatggatatt tacggtttcc
     8401 gcgtcattgt caacagcatt cccgcctgtt atgccgcact cggcacattg cacaccctct
     8461 atcagcccaa gcccggacgg ttcaaagact atatcgccat tccgaaaagc aacggttatc
     8521 aaagtctgca tacgactttg gtcggcccct acggcttgcc gattgaagtt cagatacgca
     8581 cgcgggaaat ggatgccgtg gcggaaggcg gtattgtcgg ccattggagc tataaatcag
     8641 attccaaaac ggtcgatcag gcagtgctgc acaccaatca atggttgaaa aacattttag
     8701 acttgcaggc aagcagcgcc aatgccattg agtttctcga acacgtcaaa gtcgatttgt
     8761 ttccgaacga aatctacatc cttactccga aaggaaaaat cctaactttg cccaaagggg
     8821 caacgcctgt cgattttgct tatgcggtgc ataccgacat tgggcataaa acagttgccg
     8881 cgcgtgtcaa taatgtgatg atgccgctgc gtacgaagct caaaaccggt gattctgttg
     8941 aaattatcac atccgaacac gccaaaccca atcccgcgtg gttgaatttc gccgtgtccg
     9001 gcagggcgcg cagtgccata cgccaatata ttaaaaatct taaccggcac gatgcggtcg
     9061 ttttgggaga gagcctctta caaaaagcct tgtccagttt gctgcccaaa gatgtcctgc
     9121 tttcagacgg catcaaagaa aaataccttg ccgatctcaa cgacaaacag acttcatttg
     9181 aggaagtgct gtacaacgtc ggtatggggc ataccctgcc tgtttatgtc gccatgcata
     9241 tcgccgaatt ggcaggggag catttcggca gcgaggtcag gctcagttcc attaaagttg
     9301 atggtcagga aagcgggcat attcattttg cagagtgctg ccaccctgtt cccggcgatt
     9361 ccatccgttt gctgttggtt aagggaaaag gcatgattat ccatagggat acctgcccga
     9421 cgttgttgaa gtccgatccc gaacagcagc tggatgcaga ctgggaaaat atgaacgggc
     9481 agaactaccg tgtcgggctt caagtccaat cggaagacag ccacggcctg ttggcattaa
     9541 tggcgcaagc gatttccgat tccggtgcgg acatcgagtc ggtcgaaaca ccgtctaaat
     9601 cccagtcggg aacggaaggt tttgtcgaat tcaaattctt attgaaagtc aagaatttgg
     9661 atcagttgaa tcagattatt caaaatctgc acagcatccc atatatccgc aaagtcatca
     9721 gaagttgaag caggtaaaac gggttgcctg ttaatcaaaa ggcaacccgt tttatggttt
     9781 atccgttcaa ttgcttattt gttcaaatat acggcaagcc tttcaacaat ccgcattgcc
     9841 gcttcatctt tacttgtttc ggggaaagac aactccgcat catcatctat gataacaatt
     9901 tggttggttg tcttgcccat cgcgattgaa acatcattgg caactatcac cggtattttt
     9961 ttcttaatac gtttttcccg cgcatatgcc attacattct ccgtttcagc ggcaaaaccg
    10021 atgcagaacg gcgggttcgg caatgaggca atagaagcca aagtatcggg gttctcatcc
    10081 aattcgatgg ataacggttt ggcattttta tcttttttga atttttgcgt actcctgttt
    10141 ttaaccttat agtctgagac ggcggcaaca gaaataaaag catcttgttt ttcaatcaaa
    10201 cgatgtactg cgcgatgcat atcttcggca ctgacggctt gaaccgtatc ggatatgccg
    10261 aaaggcagcg tggtttgaag ctgtccgtga atcaggctga tttccgcacc ggcggcacgg
    10321 cacgcccgcg ccaaagctac gcccattttt ccgctggagg tatttgtgat gcccctgaca
    10381 gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga ttttcttgtc ccttaaaatt
    10441 ttcggtgtcc acaagtctgg aagcaaatcc agcaattcgg caggttccgg catccttccc
    10501 ataccatttt ctctgcaagc ctgttcgccc aagcccggca tatagacagt aatgccgtct
    10561 gaaaccagtt gtgcgatatt ccgttggttg gcaggattca gccacatttc cacattcatc
    10621 gcgggagcga tggcaagagg gcatttccgt gcggctgcca gattggtcag taggttatct
    10681 gccacgccgt tacagatttt tgccacggta ttcatacttg ccggcgcaat cagaaaaaca
    10741 tccgcattcc gggtcaggtt gatatgttcc ataccgtttg aaccgttgcc gccgtgcgtg
    10801 tcggtcagaa cgggattgcc gctcaaagcc tgaaaagtca gcggagaaac aaattcagtt
    10861 gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt ttttcagcag tcgcaccaac
    10921 tcgcaagact tatacgccgc aatactgccc gttacaccta aaagaatatg tttgcccatt
    10981 ttgacaattt ctaattacga agaaaaaaca gcagccattc tataacaaag tacggataca
    11041 ttagttaccg tttggatacg aaatgccgtc tgaagccccg agtttcaggc ggcatattca
    11101 caaaggcgca tcagccggag gagaagagga gggggg
//
LOCUS       DAWTHD010000077        11113 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWTHD010000077 DAWTHD010000000
VERSION     DAWTHD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.077"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(93..1121)
                     /gene="trpD"
                     /locus_tag="WM704_001556"
     CDS             complement(93..1121)
                     /gene="trpD"
                     /locus_tag="WM704_001556"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFA9340947.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1185..1775)
                     /locus_tag="WM704_001557"
     CDS             complement(1185..1775)
                     /locus_tag="WM704_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFA9340948.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2186..4462
                     /locus_tag="WM704_001558"
     CDS             2186..4462
                     /locus_tag="WM704_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9340949.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(5002..5781)
                     /locus_tag="WM704_001559"
     CDS             complement(5002..5781)
                     /locus_tag="WM704_001559"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFA9340950.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5935..8784
                     /gene="uvrA"
                     /locus_tag="WM704_001560"
     CDS             5935..8784
                     /gene="uvrA"
                     /locus_tag="WM704_001560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFA9340951.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEEVAKVKGSYTGKYLKVVLK"
     gene            8802..9443
                     /locus_tag="WM704_001561"
     CDS             8802..9443
                     /locus_tag="WM704_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFA9340952.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9447..10580
                     /gene="dcm"
                     /locus_tag="WM704_001562"
     CDS             9447..10580
                     /gene="dcm"
                     /locus_tag="WM704_001562"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFA9340953.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
     gene            10888..11052
                     /locus_tag="WM704_001563"
                     /pseudo
     CDS             10888..11052
                     /locus_tag="WM704_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2928 a         3124 c         2585 g         2476 t
ORIGIN      
        1 aggaagggct tttttgcaaa ggcctcatgc cgtctgaaac gccaattccg ccgttcagac
       61 ggcatttgtt gctttatatc aagtttgccg gcttaggcga attgccgtgt aaaaccgaca
      121 aactcctctt ttttcgcctt cgccctacct gaatcgatgc cttcccgtgc ggcagatatg
      181 ccgtctgaaa gcgaagcagc gatatttccg gcatataagg cggcggcggt attaagcaat
      241 acgatatcgc gcgccgcccc ttctttcccg tccagtacct cgttcatttt caacaaagat
      301 tcctgagtat tggcaacttt gatttcatcc aaattgcggc gggtttcgat accgaaatct
      361 tctgggcgga tgtcgtattc gctgattttt ccgtctttga gttcggcaac gcgcgttttg
      421 cccgtcagtg taatttcatc caaaccgccc tccccgcaaa cgaccaaaac gtgttttgag
      481 ccgagttgtt gcaagacccg cgacaaaatg ccgcacaaat cggtgtggaa cacgcccaaa
      541 agctggttcg gcgcgcccgc aggattggtt aacggaccca atatgttgaa aatgcttcgg
      601 aaaccgagcg aacggcgcac cggggcgaca tggcgcatgg cactgtggtg attgggtgcg
      661 aacataaacc ctataccggt ctgcctgata ctttgggcaa tctgttcggg agtcaggttg
      721 aggtttgcgc ccatttgctc catcacgtca gccgcaccgc tggaggaaga gaccgaccgg
      781 cctccgtgtt tggcaacctt tgcgcctgcc gctgcagcaa caaacatcga agtcgtcgaa
      841 atattgaagg ttttcgcgcc atccccgccc gtaccgacga tatcgaccag cccgtccgca
      901 tcctccagcg gcacttttga cgcaaactcg cacatcacgg cggcggcggc ggtgatttcg
      961 gaaacggttt caaccttgat acgcaagccg gtcaaaatcg ccgcaatttg ttcgggcggc
     1021 acttttccgc tcatcatttg gcgcatcaag tcggtcattt catcgtaaaa caactcgtta
     1081 ttgctgatta atcgttcgat agcctgttgc ggtgtaatca ttttttgtcc tccgttcaat
     1141 attcggacga aaatgccgcc cgaagggctt cagacggcat cacgtcagat tttttgcggt
     1201 ttgaagtttt gaaattcaac taaaaaattg ttcaacatat catgtccgcg ttcggtcaag
     1261 agggcttcgg ggtggaactg cacgccctcg acggcatatt ccttatggcg cacgcccata
     1321 atttcgccgt cttccgtcca cgccgtgatt tccaagcaat ccggcagcgt gccgcgttcg
     1381 ataacgaggc tgtgataacg cgtacaggta accggattgg gcaaaccctt aaacataccc
     1441 ttgcccgaat gggacacggg cgacacctta ccgtgcatca aggtttttgc ccgtaccaca
     1501 tctccaccga acgcttcgcc tatcgtctga tgcccgaggc acacgcccat aatcggcagc
     1561 cggccggcaa aatggcgcat ggcttctact gaaatacccg cctccttagg ggaacacggt
     1621 ccgggaccga taacgagata ttgcggattc aatgcctcga tttcctccaa cgtaatatca
     1681 tcgttgcggc gcacggcgac ttcctgcccc aattctgcga aatactggac gatgttgtaa
     1741 gtaaaactgt cgtaattgtc gataaacaaa agcattttgt gattaaccta ttgatttata
     1801 atttgtttat tattaactgc atttccgata cccccatcca taccccctta tatcttagcg
     1861 tgcccgatgc gccctcgtga atctggcgca gagcctcgcg ttctgacaag tacgccattt
     1921 tgccaccttt tcgaccgttc gggaacgtac cgtttttatg ccctcataaa ttttggttat
     1981 ccgatagcta acatatagcg gcgttcgtgt tcacatcatt aacttcccta aaattagggt
     2041 taatacaaat taacgccatt aaatatcaca accgtgtcat ttgaaaaact tttgcctatg
     2101 ccctctgagt attctcaaaa tcttatgata taaaaaatgc aatgttatat aataacaaac
     2161 ttttgaaatg cgacgagaag ccaaaatggc acaaatcaca ctcaaaccca ttgttttatc
     2221 aattctttta atcaacacac ccctcctcgc ccaagcgcat gaaactgagc aatcggtggg
     2281 cttggaaacg gtcagcgtcg tcggcaaaag ccgtccgcgc gcgacttcgg ggctgctgca
     2341 cacttcgacc gcctccgaca aaatcatctc cggcgatact ttgcgccaaa aagccgtcaa
     2401 cttgggcgac gctttggacg gcgtaccggg cattcatgcc tcgcaatacg gcggcggcgc
     2461 atccgctccc gttattcgcg gtcaaacggg cagacggatt aaagtattga accatcacgg
     2521 cgaaacgggc gatatggcgg acttttctcc cgatcacgcc attatggtag ataccgcctt
     2581 gtcgcaacag gttgaaatcc tgcgcgggcc ggttacgctc ttgtacagct cgggcaatgt
     2641 ggcggggctg gtcgatgttg ccgatggaaa aatccccgaa aaaatgcctg aaaacggcgt
     2701 atcgggcgaa gccggattgc gtttgagcag cggcaattta gaaaaactga catccgcagg
     2761 catcaatatc ggactgggca aaaacttcgt gctgcatacc gaaggcttgt accgcaaatc
     2821 gggcgattac gccgtaccgc gttaccgcaa tctgaaacgc ctgcccgaca gccatgccga
     2881 ttcgcaaacg ggcagcatcg ggctgtcttg ggtaggcgaa aaaggcttta tcggcgcagc
     2941 atacagcgac cgtcgcgacc gctacggcct gcctgcccac agccacgaat acgatgattg
     3001 ccacgccgac atcatctggc aaaagagttt gatcaacaaa cgctatttgc agctttatcc
     3061 gcacttgttg accgaagaag acatcgatta cgacaatccg ggcttgagct gcggcttcca
     3121 cgacggcgac ggtgcacacg cacacaccca caacggcaaa ccgtggatag acctgcgcaa
     3181 caaacgctac gaactccgcg ccgaatggaa gcagccattc cccggttttg aagccctgcg
     3241 cgtacatctg aaccgcaatg actaccacca cgacgaaaaa gcaggcgatg cagtagaaaa
     3301 cttcttcaac aacaaaacac acaacgcccg tatcgagttg cgccaccaac ccataggccg
     3361 tctgaaaggc agctggggcg tgcaatattt gggacaaaaa tccagcgcgc tttccgccat
     3421 tcccgaaacc gtccaacaac cgatgttgat tgacaacaat gtccgccatt acagcttttt
     3481 cggtgtagaa caggcaaatt gggacaactt cacgcttgaa ggcggcgtac gcgtggaaaa
     3541 acaaaaagcc tccatccggt acgacaaagc attgattgat cgagaaaact actacaacca
     3601 gcccctgccc gacctcggcg cgcaccgcca aaccgcccgc tcgttcgcac tttcgggcaa
     3661 ctggtatttc acgccacacc acaaactcag cctgaccgcc tcccatcagg aacgcctgcc
     3721 gtcaacgcaa gaactgtacg cacacggcaa gcacgtcgcc accaacacct ttgaagtcgg
     3781 caacaaacac ctcaacaaag agcgttccaa caatatcgaa ctcgcgctgg gctacgaagg
     3841 cgaccgctgg caatacaatc tggcagccta ccgcaaccga ttcggcaact acatttacgc
     3901 ccaaacctta aacgacggac gcggccccaa atccatcgaa gacgacagcg aaatgaagct
     3961 cgtgcgctac aaccaatccg gtgccgactt ctacggcgcg gaaggcgaaa tctacttcaa
     4021 accgacaccg cgctaccgca tcggcgtttc cggcgactat gtacgaggcc gtctgaaaaa
     4081 cctgccgtcc ctacccggca gggaagatcc ctacggcaaa cgtcccttca tcgcgcagga
     4141 cgaccaaaac gccccccgca ttccggctgc gcgcctcggc ttccacctga aaacctcgct
     4201 aaccgaccgt atcgatgcca atttggacta ctaccgcgtg ttcgcccaaa acaaactcgc
     4261 ccgctacgaa acgcgtacgc ccggacacca tatgctcaac ctcggtgcaa actaccgccg
     4321 caatacgcgc tatggcgagt ggaattggta cgtcaaagcc gacaacctgc tcaaccaatc
     4381 cgtttacgcc cacagcagct tcctctctga tacgccgcaa atgggccgca gctttaccgg
     4441 cggcgtaaac gtgaagtttt aaaatcggac aggcaaacaa aaaaggtttt cagacggcat
     4501 tctgaaaacc tttttttctt tttaatcaac ttgttaggaa atatggcaaa gacaaggaac
     4561 aaaccactgt gccatcattc ccattttctt tgtttcatca ttcttgcttt cacgccgtca
     4621 ttctcgcaaa agcagaaaaa tcaaaagcaa aaacccaaga tcccatcatt cccgcgcagg
     4681 cgggaatcca attcattcgg tttcagccgt ttccgataaa tcaccgtaac gttaagttcc
     4741 cggattccca ctttcgtggg aatgacggcg taaaggtttc tgttttttaa gttttaggta
     4801 actaatgagt cgtcattccc gcgcaggcgg gaatccaatt cgttcggttt ctgttatttc
     4861 tgataaatca ccgtaacgtt aagttcccgg attcccattt tcgtgaacat aacggtgtga
     4921 acattagcaa aaattcaaaa aaccaaactt cctcattcgt gtgaatgatg gtactgatat
     4981 tttctatttc agttcgcaaa attaatcttt caacacatct tgcacagccg cctgaatctt
     5041 agccgccgcc gcttcgattt gctgctgttc ggcagatggg tttgccgctg tttcaaccga
     5101 agcagcttgt gaaacaggat cggcttgaga atcagtcagc ggcaaacgcg ccaatacggt
     5161 ttttacgccg gttactttat caccaatcgc cacttgcgcc tgcgcatcga caggcagata
     5221 cacatccacg cgcgaaccga agcggataaa gccatagcgt tcgccgcggg acagtttcgc
     5281 acctgcttgg gtgtaacaca aaatacggcg tgccaccaga ccggccactt gaacaaaagt
     5341 aatttcacga ccggaagccg tagtcgccaa aaccgcgttg cgttcgtttt ccgtgctggc
     5401 tttgtccaaa tccgcattca cgaatttgcc tttgttatag accactttcg ttaccgtaca
     5461 atcggcaggc gatttttgcg aatgcacgtt gaacacgttc ataaaaatac tgattttcaa
     5521 agcatcgaca tcacgatacg gatcgcgtgc gcgctcgacc accacgatac ggccgtcaac
     5581 cggactcaac accgcttcag gattttgcgg aatttcacgc gcagggtcgc ggaaaaactg
     5641 caatgcaaat acggtaaaca cccaaaacgg caaagaccac cagccgcagc acatcgacac
     5701 cagcaggctc aaagccaaac cgccgccgat aatcggccag ccctcacggg cgataatcgg
     5761 gtggggataa agacggttca tttgtcatcc ttttattcaa atatattaaa tagcgacatt
     5821 ataacggaat ccgccgcgtt ccgccgacaa atgccgtctg aacgcggctt cagacggcat
     5881 ccgagaaagt gctacaatat cggttttcct ttattttttc aggcggcatc cattatgtgc
     5941 aaccatcatc cccgacattc gcacgacaac gacaccatcc gcattcgcgg cgcgcgcacg
     6001 cataatttga aaaacatcga tttggacatt ccccgccaca agctcgtggt ggtaacagga
     6061 ttgtcaggca gcggcaaatc atcgctggcg tttgatactt tatatgccga agggcagcgg
     6121 cgttatgtcg agagcctgtc tgcctatgcg cggcagtttt tgcagatgat ggacaaaccc
     6181 gatgtcgatt tgattgaagg cctgtcgcct gcgatttcca tcgagcagaa atccaccagc
     6241 cacaatccgc gttccaccgt cggcacggta acggaaatcc acgactacct gcgcctttta
     6301 tacgcccgtg tcggcacgcc gtattgcccc gaacacaagc tgccgctatc cagccaaacc
     6361 gtatctcaaa tggtcgatgc cgtattaaaa ctgccggaag atacgcgcgt gatgattctg
     6421 gggccggcgg tgcgcgagcg taagggcgag tttgtcgatt tctttgccga cttgcaggcg
     6481 caaggctttg cccgtgtgcg cgtggacggc gaagtctatc agcttgacga agtaccgaag
     6541 ctggaaaaaa acatcaaaca caatatcgac gtcgtcatcg accgcgtgaa agtgaaagcc
     6601 gacatcaaac aacgactggc ggaaagtttt gaaaccgcgc tgcgccacgg caacgagcgc
     6661 gcgctggcga tggaaatgga cagcggcgaa gaacattggt tttccgcgcg ttttgcctgc
     6721 cccgtatgct catacagcct gcccgaattg gaaccgcgcc tcttttcgtt caacaacccg
     6781 atgggttcct gcccgacttg cgacggcttg ggcaacacca atttcttcga ccccgaaaaa
     6841 gtagtcgccc atcccgaatt gtcgctggca accggcgcga ttgacggttg ggacaaacgc
     6901 aaccaattct atttccaaat gattcaatcg ctggcgcatc attataaatt tgatgtcaac
     6961 gtcgcttggg aaactttgcc tgaaaaagtc aaaaaagtcg tgttgcacgg ctcgggcaaa
     7021 gaagtcattg atttcaccta cctgtccgaa cgcggcacca ccttcaaccg cagccacgcc
     7081 ttcgagggca tcatccccaa tctcgaacgc cgctaccgcg aaaccgacag cgaaaccgtg
     7141 cgcgaaaaac tgcgcgagta ccaaaatcac cgtgcctgcc cgagctgcgg cggcgcacgt
     7201 ttgcgcaaag aagcacgcta cgtttacgtc ggcggcgaac cgttgcacga agtctccgcc
     7261 tggccgctga ccaaaaccca ccgattcttc gaaacgctgg atttggacgg caacaaaaaa
     7321 cagatcgccg aaaaaatcct caaagaaatc accgagcggc tcggcttctt gattaacgtc
     7381 gggctggatt acctgaattt aagccgttcc gccgaaaccc tttccggcgg cgaagcccag
     7441 cgcatccgcc tcgccagcca aatcggcagc ggcctgaccg gcgtgatgta cgttttggac
     7501 gaaccctcca tcggcctgca ccagcgcgac aacgaccgcc tgctcgccac cctcaaacgc
     7561 ctgcgcgatt tgggcaacag cgtaattgtg gtcgaacacg acgaagacgc catccgcgaa
     7621 gccgatttcg tcgtcgatat gggccccggc gcgggcgaac acggcggcaa cgtactgatt
     7681 gccgacacac ccgaaaatgt cgccaaatgc gaaaaatccg ttaccggaca atacctcggc
     7741 ggcaaaaaat ccattgccgt gccgtctgaa cgcacgcccg tcaatcccga ccgaatgctc
     7801 gtcctcaaag gtgcgcgcgg caacaacctc aaaaacgtca ccctcgagtt gccgctcggt
     7861 ttgattacct gcatcaccgg cgtatccggc agcggcaaat ccaccctgat taacgacacc
     7921 ctcgccaaaa tcaccgcccg cgaactcaac cgcgcccaag aagaacccgc cccatacgac
     7981 gacatccgcg gcctcgaaca cctcgacaaa gtcatcaacg tcgaccaatc ccccatcggc
     8041 cgcaccccgc gctccaaccc cgccacctac accggcctgt ttacccccat ccgcgaactc
     8101 ttcgccggcg tacccctctc gcgcgaacgc ggctacaacg tcggcagatt ctccttcaac
     8161 gtcaaaggcg gccgctgcga agcctgccaa ggcgacggcg taatcaaagt cgaaatgcac
     8221 ttcctgcccg acgtgtacgt cccctgcgaa gtctgccacg gcaaacgcta caaccgcgaa
     8281 accctcgaaa tccaatacaa aggcaaaaac atcagccaag tcctcgacat gacggtcgaa
     8341 gaagcccgcg aatttttcga cgccgtcccc accgtatcgc gcaaactgca aaccctgatg
     8401 gacgtaggtc tcggctacat ccgcctcggc caatccgcca ccaccctctc cggcggcgaa
     8461 gcccagcgcg tcaaactcgc cttggaactc tccaaacgcg acaccggcag aacgctctac
     8521 atcctcgacg aacccaccac cggcctgcac ttcgccgaca tcgccctgct gctggaagtc
     8581 ataggccgtc tgaaaggcaa aggcaactcg attgtgatta tcgagcataa tctggatgta
     8641 attaaaaccg cggattggat tgtggactta gggccggaag gcggcgatgg tgggggtaag
     8701 gttatcgcga aaggtagccc tgaggaagtg gcgaaggtta aggggagtta taccgggaag
     8761 tatttgaaag tggttttgaa gtaaacgaac ggatatagaa aatgaacaat aattttgacc
     8821 gcagcaagca tatctatcat aacgaagctt ttgtagaact ggttaaagat gccatacgct
     8881 tttttcatgg tacgcctgtt catacacttc ctccacccaa acagttcaaa ggtgcagggg
     8941 tttatgcgat ttactatatc ggtaacaatc ctttgtacaa acaatatgcg gactggaaca
     9001 gattatccta caatgcgcca atttatgttg gcaaggccgt ccccaaaggc tggcggcaag
     9061 caagaaattc tgataatgcg ctaaaccaat caaccgaact gttccaccgg ttgaaagaac
     9121 acagcaggag tattgccgct gtttctgatt tagatccttc cgatttcatg tgcagattcg
     9181 ttatctttga aggtgccggt tctgacatga taggcacaat cgaagctgcc ctgattaaat
     9241 tacacaaacc tctatggaac tcttgtgtcg atggtttcgg aaaccacgac cccggaaaag
     9301 gccgttacga acaagccaaa tcggattggg atgtccttca tagcgggcgg gtttgggcag
     9361 ataggctaaa cggcataccg aatagctatg agtccatact tgaaaatatc aacacacatt
     9421 tagagataat aaagagaaaa taacctatga agtctttgga aattttttcc ggcgcgggcg
     9481 ggttggcaaa aggccttgaa cttgcaggat tccaacatgc atcttttatt gaactcaata
     9541 aagatgcatg caattcttta agaagcaact ttaaccccaa gcttgtttat cagggagatg
     9601 tggcagattt tgatctttcc agtcaagaag gcatagaagt cattgcaggc ggtcctcctt
     9661 gccagccctt ttcattgggc gggaaacacc ttgcccatga agacagaagg gatatgtttc
     9721 ctcatgcggt cagatatgtg gagtattacc gccccaaagc ttttatcttt gagaatgtaa
     9781 aaggactttt aagaaaatca ttcgctgatt attttgaata tatcctactt cggcttactt
     9841 atccgaatct tggtattttg cagaatgaag attggaaagg ccatctgaca cggctgaaag
     9901 aaattgaatt caatttatat aaaggaatca aatataaggt ttcatatcaa ttgttgaatg
     9961 cagctgatta cggagtgccg caaaaacgcg aacgcgtggt tatcgtaggt atccgggccg
    10021 atttggatat tgattggaaa ttcccaaaaa gaacacattc cgaagacagg ttgaattggg
    10081 aaaagtatgt aacgggggaa tactgggaaa aacacaacga acccaaaaga ttcaataaag
    10141 atattgctga aaagttacaa aaaaaatacg gtatattcga accagaaaaa aaaccttggc
    10201 aaacggtaag ggataccttg tccgacatcc cgcatccttt ggggaatcat aaaattacag
    10261 gacatgaata tagggatggc gcaagaattt atcccggaca cacaggaagc gggatagacg
    10321 aaccgtccaa aaccattaaa gcaggtgggc atggcgttcc cggcggagaa aatatgattc
    10381 gttatgatga tggaacagtc agatacttta ccagctatga agcaaaactt cttcaaacat
    10441 tccctgaaga atttgtcatt tctggagctt ggggagaagc aatgcgacaa attggcaatg
    10501 cggttcctgt caaattgtcg gaaattttag gcaaacatct gatgggggtg ttgtcggaga
    10561 aaagcagcct gcacaactaa cccgcccact gcgtgtgtgc ggtacgcacg cggttgcttg
    10621 attgacggtt tgccgtttca acttttaact tcgttgaagc tagtgctttc agacggcctc
    10681 caatgtcgtt tggaaacatc aggtccaaca aagttaaacc ttaaacaaca aaccattcct
    10741 tctccaacac ccaaaccaca aacaaaaatc ctatggcgcg ttatcgcaga aacttcattg
    10801 ccggcggcac attctttttc accgtcaaac ccgccgcccc gaaatcgcgt ctgcttgtcg
    10861 gacatatcgg cttcgcgtgc ggcttatatg gatgtgcaaa aacaatatcc ctttgaaacc
    10921 gtcgccgtat gcgtgctgcc gaaccatatt cacgccattt ggacgctgcc gcccgacgat
    10981 gcggattatt ccctactccg gcggctgatt aaaaccaaat tctccgcata ttcccctcat
    11041 actaaaaact taggggctgt actagattat ccctaaattc cacaccgatc ccgcaggatt
    11101 tttagctgcc ggg
//
LOCUS       DAWTHD010000078        10899 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWTHD010000078 DAWTHD010000000
VERSION     DAWTHD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.078"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..188)
                     /locus_tag="WM704_001564"
     CDS             complement(<1..188)
                     /locus_tag="WM704_001564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340954.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
     gene            313..996
                     /locus_tag="WM704_001565"
     CDS             313..996
                     /locus_tag="WM704_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFA9340955.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            1191..1460
                     /locus_tag="WM704_001566"
     CDS             1191..1460
                     /locus_tag="WM704_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340956.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(1816..3008)
                     /locus_tag="WM704_001567"
                     /pseudo
     CDS             complement(1816..3008)
                     /locus_tag="WM704_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(3199..3288)
                     /locus_tag="WM704_001568"
     tRNA            complement(3199..3288)
                     /locus_tag="WM704_001568"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3252..3254),aa:Ser,seq:cga)
     gene            complement(3365..3455)
                     /locus_tag="WM704_001569"
     tRNA            complement(3365..3455)
                     /locus_tag="WM704_001569"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3419..3421),aa:Ser,seq:tga)
     gene            complement(3538..4317)
                     /gene="yaaA"
                     /locus_tag="WM704_001570"
     CDS             complement(3538..4317)
                     /gene="yaaA"
                     /locus_tag="WM704_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFA9340957.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTSFANLRGKNLYEFWGGIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(4473..5660)
                     /gene="dapC"
                     /locus_tag="WM704_001571"
     CDS             complement(4473..5660)
                     /gene="dapC"
                     /locus_tag="WM704_001571"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFA9340958.1"
                     /translation="MNTLLNRLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVC
                     LYR"
     gene            complement(5738..6190)
                     /gene="dut"
                     /locus_tag="WM704_001572"
     CDS             complement(5738..6190)
                     /gene="dut"
                     /locus_tag="WM704_001572"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFA9340959.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     GTLLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            6356..7064
                     /locus_tag="WM704_001573"
                     /pseudo
     CDS             6356..7064
                     /locus_tag="WM704_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            7061..7369
                     /locus_tag="WM704_001574"
     CDS             7061..7369
                     /locus_tag="WM704_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFA9340960.1"
                     /translation="MKNLLSFPSFLLFSCMLAVTCPTRLIGFFALRNRTLSRRAQTVM
                     EAAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLLTVLIGVGSSGISGWLMA
                     "
     gene            complement(7439..7912)
                     /locus_tag="WM704_001575"
     CDS             complement(7439..7912)
                     /locus_tag="WM704_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFA9340961.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQIIKSKLEAFVLGYKMNPKIAKKYSVSVAFANTEKPRA
                     YRLVGVPNAGTGYTLSVWMNSVGDGYKCRDAASAWAYSDTLSADSGCEAFSNRKK"
     gene            complement(7914..8522)
                     /locus_tag="WM704_001576"
     CDS             complement(7914..8522)
                     /locus_tag="WM704_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFA9340962.1"
                     /translation="MRKQNALTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYTADSKVTFSENCENGLCTAVNVRT
                     NDANEETFDNIVVKGKPTVEAVKRPCPAKSGKNSTGLCIDNQGVEYKKGTGNVSKIPR
                     YIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            complement(8501..9469)
                     /locus_tag="WM704_001577"
     CDS             complement(8501..9469)
                     /locus_tag="WM704_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFA9340963.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSDVAQKNRL
                     FSLKMNSTNKVISNGTDNKLIPIAESLNIGYQGFAQVRPALIFQYGIDDVNASAETTV
                     VSSCGVISKPGKQIPTLEDAKKELKIQNSGKEQNGNIARQRHVVNAYAVGRIAGEEGL
                     FRFQLDDKGKWGNPQLLVKKVKRMDVRYIYVFGCPEDEDAGKEEQFKYTDKFDKSRDA
                     VTPAGVEVLLDSGLDAKIAASSDNSIYAYRINATIRGGNVCANRTL"
     gene            complement(9466..10080)
                     /gene="pilV"
                     /locus_tag="WM704_001578"
     CDS             complement(9466..10080)
                     /gene="pilV"
                     /locus_tag="WM704_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFA9340964.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGPYTPTPSGGDFKLNNN
                     NLISKKDLAKAQLDRFDYELRNALPDAVAIHYAVCKDSSGGAPTLSGGTFSPNCDNKA
                     NGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGHE"
     gene            complement(10112..10774)
                     /locus_tag="WM704_001579"
     CDS             complement(10112..10774)
                     /locus_tag="WM704_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HFA9340965.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMATIALPNMSGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGASNNRCDFSKKGWGMLAFGDKNDNK
                     AYDGDATDVFLRSVVLNDTDDSRINYTFNHIAFGSSQPKADRVVWTFNQNGTFGYLPD
                     QNLKNNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCRKNDTRAVC
                     KH"
BASE COUNT         2877 a         2853 c         2437 g         2732 t
ORIGIN      
        1 ttgccgccaa agtccgccga tactgcggcg aacatccggg cgtatttgac ggcgcggcag
       61 gaagcaggca gcttgaacgg tatattaagc cgtctgaatt tcacgccgtg gaaatacagc
      121 ccgaagcctg caaagccttg ttgcaaaact accctgccgc ggccggtaat ctcggcaggc
      181 ggaaacaaaa aaagccccga gttcgtaagc gtatcagccc tagccgaatg gcccgaccga
      241 atcgaaagca aagcgaaaga cgaatgggca agggtgcgaa attaaaatac cggacaaatt
      301 aaagggttta atatggataa agagaaagtc ttagacaaaa tcaagaaatg tttggctttg
      361 ggtcggtcgg caaatgaaca tgaagccgca caggcgttaa ggcaggcgca ggctttaatg
      421 gaaaagtaca aagtaaatgc cgaggatatc gccttatcaa aagtctccga acagaaagcc
      481 gatcggaaaa tggcttttaa attggcaggc tggcaatggg gcgttgcaaa tatgattgcc
      541 gatatattcg ggtgcaaatc ttaccaacgc gggaaaacaa tgatgtttta cggcattgga
      601 aatcgagcgg aaacctcagc ctatgccttt gatgtagtct atcgtcagat ttccgccgac
      661 cgccgcaaat ttttgaaaac ctgccgggcg ggaaagccct cgcacagaac ttatcttgcc
      721 gaccgatttt gcggcggttg gattgcgagc gcttgggaaa cagtcaaaaa atttgagatg
      781 tcagatgaag aaaaagccat tatggacgga tacaaaaaga aagaatatcc ggatatggcc
      841 gaagcaagga caagagatgc gaaatcgtca atcctacaag ggtcaaaaat ggaatatgag
      901 gcattaaccc gaggaatgga atcgggtaag caagtgaagt tacactatgc cgtgaacggt
      961 acgggcttcg taaaacaaat cggagggcaa acatgaatcg atacggcggc ggatgcggaa
     1021 acttcggcgg attggggaaa ggatatgccg aatatgaaag acgcaattgc cgcatgccaa
     1081 gccgtaaaaa agcggaaacc cggacaaatc ggaatcaacg gaaagaggat tgaagacatg
     1141 gaatacgaag tattcaataa acgcccgtca aaaaagcggt cggcgaagca atggagtctt
     1201 ccgacgtaca agggcggatg gaaacactgg catttgcaac cgccccgaaa aaaggcggaa
     1261 tccggaaatc cggattcggc cgcgttattc taatcaaaag cgggcatata gttattcata
     1321 acgctgatgg cgacagcttt gccaccggct acggcgtgat tgccgccgac atcgtcagtc
     1381 ccgatttgaa aacagacttt gacatattct tcccgccgga aagcttgttg cgatataaaa
     1441 aaatcgggaa gaagaaatag gcagacaaac gacagagccg taaaaacggc gcaagattaa
     1501 accgaacagg agagtaaaaa aatgaacatc accaaaccgg gcgcaaggat agaccgcccg
     1561 accatccgcg aactgattgc ctacgcaacc tgccgcaacc accccatttc aaactcgaca
     1621 ctgctgcgta tggaaaaaga cggacggata ccgtgccgtc tgaacctccc tcgcatcccc
     1681 cgtatgggac acccgcgaag ttttggaagc attagggttg cagcaataaa aaaacgcccg
     1741 attagggcgt tttcacataa tgcggaaaca gctttcttat ctacatttcc acataacgca
     1801 aaacacacaa aaaaatcatt ttccgttgtc ggcaagcctc aaaacatcat tcccccaccg
     1861 ttccggcgca tccttacgct cctcccaaag ctccgagcgg ttgtaagccg tttgcgtcct
     1921 cgtccccaac gaatgggcaa gcagcttttc cgccacatca ttatacacct tcagaacctc
     1981 gcgcaaataa gtacgcgcaa gcgagcgcaa accgtgcgcc gtcgtatcca gccccgaccg
     2041 ccgcatcgcc acgcgcggac tttccttatt gatatgccct tgaaaattca cactttcaaa
     2101 cacaaaccgc ccattcacat tcagcccccg cgcctgatcc aacacccacc gcatcgcccg
     2161 cgacagcggc acatcatgcc cccgccgctc ctttttccgt tcggcgggga tacgccaaac
     2221 ctccccgtcc aactccgacc actccatcaa cgccgcctcc cgaatccgcg tcatcgtcaa
     2281 cagcagccaa tagatacaaa accgcgttac aggatggata tgcaccctgc ccgcataaac
     2341 cgcaaattca ttgcgctgct ccaaaaaatc aatcaggcgc ggcaattcgg acggactcaa
     2401 cgccgtcatg tttttggttt tcacccgttc aaacacaccc gaaccgattt gcgcgacagg
     2461 gttgatttcc atcattcccg aaccgaccgc aaacgcaaac accatcttca gactgttttt
     2521 cgttttgcgc aacgtatcaa cgataccgcg cgcctccatc acacgcaggc agccgaccac
     2581 gtccgccgtc ctgacttgac ggatatcaag attgccgata gccggaaaaa cccaccgttc
     2641 aaaattccgc ataacctgtc cggcatactt ttcagaccgc cccttcgacc aacgcacaaa
     2701 ccaatcacgc gccaccttct caaaagcaaa atccgcccgc accttcttat tgacgacatt
     2761 ttccccgtgt gcccgttttc ggcgcacctc ctcccgccat tcccgcgcat cggtcagcga
     2821 aaaatcagga taccgcccca gcgaaattgt ctgctgcctt ccatcctgca caaacgacag
     2881 cttccaactc cgtccgcccg tcgggtagac ccacaaagcc agcccccgtc cgacaactta
     2941 tacagcttat cgcgcggctt cgcattttta acctgatttg ccgacagcgg cgtaatgatt
     3001 ttcgccatta tggtaaattc ccgtttgtcc aaaatttacc atataaatta ccataaaaaa
     3061 atgcaataag aagatgccgc aagattgcaa atgatttgcc ttgaagcgtt atgataggtc
     3121 aagccgatga tttttatatg gtaatcaaca aaacttgaac aaaaaagaac cgccctgaat
     3181 cagggcggtt atgttttctg gcggaaacgg tgggattcga acccacggag gatttgcacc
     3241 ctcagcggat ttcgagtccg ctgcattcag cctctctgcc acgtttccga taatgcagta
     3301 aaaccaaata aaaataacaa tattcgcggc tattgcattt ttatttggtt ttacgctcaa
     3361 aaattggcgg aagcggtgag attcgaactc acggagggct atcaaccctc gacggttttc
     3421 aagaccgttg cattaaaccg ctctgccacg cttccgtctt cttgaagagt aggaataata
     3481 atgaagttta tacattttgc caagcacttt ttttgggaaa tacttaattt attgttttca
     3541 ctttatttgt tccgaacgca taaacaccca ttcgctttca ttggaagccg cgtcattgaa
     3601 tgcgtagcct tcgtaattaa aatttttcag catttcagga tcggtaatat tgtgttctgc
     3661 cgcatagcgc accatcaatc cgcgcgcgcg tttggcatag aaactgatga ttttatattt
     3721 accgtttttt tcgtctttaa atatcggggt aatcagccgc gcccgaagtt ttttcgtgtt
     3781 gacggacttg aaatattcct gtgaggcaag gttgacaagc gtattgctgc ctgcttgggc
     3841 aagcgtgtca tttaaaaggt tggtaatgat gccgccccaa aactcataca aattcttgcc
     3901 gcgcaaattg gcgaaggacg ttcccatttc caggcggtag ggctgcatca aatccagtgg
     3961 acgcataaga ccgtacaagc cggacagcag gcggacatgg ttttgcagat attgtatttg
     4021 attggtattc aatgtgtttg catccatacc ttcgtaaaca tcgccgttga acataaagac
     4081 tgcctgtttg gcgttttccg gcgtaaacgg cgtgtgccac gctgcatttc gttcggcgtt
     4141 tagaagtgcg attttgtcgg aaacgtgcat caactctgca atttgttgag gggcaagctc
     4201 cctcagttgg cgcatcagga tttcagattc cgccaaaaga tcgggttggg taaactcgct
     4261 gacagggcaa gggtcttttt cgttaaggtt tttcgcaggg gaaaggacaa aaaacataat
     4321 ccgctcggta tagtgtcaaa cgtcggaatt ttagatttaa aagcatcctc aggcaagccg
     4381 attgattgtg atgtaaatac cgttataata aatacatctt gatagtatta aaaaaagccg
     4441 tcaggaaaag tccggacggc attttttatt ctttaccgat ataagcaaac aatggtttcc
     4501 gcagctttga cacaagttgc gacatctgca accaaagcaa tccgcacata accttccccg
     4561 ggattgccct gttcggtatc ccgtgccaaa aagcgtccgg gcaatacttg gatagcggct
     4621 ttttgccata aattgcgtgc gaatgccaaa tcgtcgccat cggggacttt caaccagatg
     4681 taaaacgagg catccggtaa tttaacgtca aatacctgtt gcaaaatggg aataacacgc
     4741 tcaaattttt cctgatacag gcggcggttg tcgataacgt gttgttcgtc attccaggcg
     4801 gcaatactgg cacgctgcac gggaatgctc attgcgctgc cgtgataggt tctgtaaagc
     4861 agaaagttct taagcagttc cgcatcaccg gcgacaaaac cggaacgcag gcccggaaca
     4921 ttggaccgct tggacaaact ggtaaacata agcaattttt gcctgcttcg ccccgactgt
     4981 gcggcggctt gcaggcagcc caaaggtttg ttgccgtcga aatagatttc ggaatagcat
     5041 tcatccgagg caataatgaa accatattta tcctgtaaat caaaaacttc tctccatccg
     5101 tccaaatccg gcacgctgcc gctggggttg ttgggcgagc agacgaacac cagtttggtg
     5161 cgttcccaaa cctcttcgga aatactgcgc caatcggggt tgaaagacgg cgcggggcaa
     5221 ttggcaaaat ggatttcacc gccgcccaaa agtgtcgcac cttcgtaaat ctgataaaag
     5281 ggattcgggc tgacgattac gggttttaga ccgtctgaaa cagggttcaa cacggtttga
     5341 acaaaagaaa acaacgcctc cctactgccc aaaaccggca gaatttcatt atccggattg
     5401 accgtcaagc cgtcataacg gcgttttaac cagttcgcac acgcctgacg cagttcaggc
     5461 agacccgcag tcagcggata tttttccaac tcgcgcaatg aggcggtcag cgcatccgta
     5521 atgactttcg gtgtcgggtg tttcggttcg ccgatatgca gtgggacggc ttccatgcct
     5581 tcaggcgcgg aaatgccctg catcgcctcg cgcagtcggg caaagggata tggtttgagc
     5641 cggttgagta aggtattcat gattttctcc ggttatcggt atatcggaag atatgccgtc
     5701 tgaagctgac gtgcccttca gacggcattc tatatcttta gtgagaaccc gtactgccga
     5761 agccgccctc accccggctg cttccgacaa attcctcgac acgtttgaag cccgcctgta
     5821 cgaccggcac gataaccatt tgtgcgatac gctcgaacgg tttgacagta aacggttcgc
     5881 tgcttctgtt ccataacgac accttcaatt ctccctgata atcggagtcg attaagccga
     5941 ccaaattgcc caaaacaatg ccgtgtttat gccccaagcc ggaacggggc agcaaaacgg
     6001 cggcatatgc gggattcgcc aaataaattg ccaaacctgt cggcacaagc aacgttccgc
     6061 ccggctgcaa aacgacttcc tcatccaaac aggcgcgcaa atccaaacct gcagaaccct
     6121 ccgtcgcata ggcagggata aaatccgcca tcctctcgtc caacactttc atttctactt
     6181 caatattcat cgtcctgctc ctgcatacgg ccggtttcaa agattggaga ttatacacaa
     6241 gccgtgttta cgctgaaaca caacaaactg cttttgtttt gaaaccatcc tcgactgtca
     6301 aaaacattct gcctgtgtat gataccgccc cgtctcttca tatataaaca ttaacatgac
     6361 gcacacagcc tctccacgtg atgaattcat acgcggcatc aaagaaagtt cgcccatgct
     6421 gattgggctt ttgccttggg cattaatact cggaatgcag gcgggcaaaa aggtatgggt
     6481 cggctggaaa tgctgctgat gacggggatg aactttgccg gcggctccga atttgcaacg
     6541 gtcaacctgt gggcggaacc tctgccgata ctgcttatcg ccaccataac ctttatgatt
     6601 aattcgcggc atatcctgat gggggcggcg cttgccccgc acatgaaaga aataccgctg
     6661 aaaaaaaccg tacccgcgct gctttttatg tgtgatgaaa gccgggccat ggcattctcc
     6721 gaaatccaaa aacggaaagc agccggtttg cctgcattca atatgccttc ttatatgggc
     6781 gcatgcttta tcctctacat tacctgatag ggtttgccgc cctcggcgcg gcagtagggc
     6841 ccgtgttcgg caatgtggcc gcttgggggt tcggtatggc gtttcctgcc gtatttctgg
     6901 ttttactgag gggaatgtgg aaaaatcttt ccgcatcaat accttgattt gtcaacctgt
     6961 ttgtcgcatc cgccgtttac ctgaccgttg acggcgcgtg gtatgttccc gccggcacgc
     7021 tgtccggcct gttgtccgct tatttttgga gtgggcggaa atgaaaaatt tattgtcgtt
     7081 cccatcattc ctgctgtttt cctgtatgct tgccgttacc tgccctaccc ggttaatcgg
     7141 ctttttcgca ctgcgtaacc gaaccttaag ccgccgcgcc caaaccgtta tggaagccgc
     7201 gccgggctgc gtcctcattt ccgtcattgc cccctatttt gtctccgaca agccgcacga
     7261 acttattgcc atcgcgttaa ccgcctttgc ggcctgccgt ttttctatgt tgcttaccgt
     7321 attgataggg gtcggctcgt caggcatctc gggatggttg atggcataat tcatttcaaa
     7381 atgcccataa aaaataccgt ctgaaaattg atcttcagac ggcattggca aaacagccct
     7441 attttttacg attagagaaa gcttcacagc cgctatctgc ggacaaggtg tccgaatagg
     7501 cccaggcaga agcggcatca cggcatttgt atccgtcgcc cacgctgttc atccataccg
     7561 acagagtata acccgtcccc gcgttcggaa caccgaccaa cctgtatgcc cttggttttt
     7621 ccgtattggc aaatgccacc gaaacactat attttttggc aattttcgga ttcatcttat
     7681 agcctaagac aaatgcttcc agtttgctct tgatgatctg attattgtcc tggggatttt
     7741 tcaaaataaa ctgtttgaga acattgttga taccgaccat ctccgtataa agctgggact
     7801 gatagccttt ttcaatataa ctttgataag aaggtatggc aatgacgctg atgatgccga
     7861 gtatcgcgac aactatcatc atctcaatca atgtaaaccc tttttgttcc attttattgc
     7921 tcatcattat tgcctacata agattgaagg acgaccacgg tattggcatt cttaccccaa
     7981 gccttggcag taacccgata aacattttgt ccgttcttca cgcctaaata ttcgataata
     8041 tagcgcggta ttttgctgac gtttcccgta cctttcttat attccactcc ctgattgtca
     8101 atgcacaggc cggtagaatt tttgccagac tttgcagggc aaggacgctt cacggcctca
     8161 acggtaggct tgcctttcac cacgatattg tcaaaagtct cttcattagc atcatttgtc
     8221 cgtacattca ctgcggtaca caggccgttt tcacagtttt cgctaaatgt aaccttacta
     8281 tccgcagtat attccaaatc caaaacctga aattcgccct cccgcaaagc cgcctcggct
     8341 aaagacaaag ccaatttcct gtctgattcg ttggcactga tccgctgctc ggtattgtag
     8401 gactgggcgg cagttacaac caaaaaggcc acgactatca tcaccatcag cacgataaac
     8461 agtgcggacc ccctctgtcc gtcagaagtc gggattcccg tcaaagcgtt ctgtttgcgc
     8521 atacatttcc cccgcgtatt gtcgcattga tacggtaagc ataaatacta ttgtctgaag
     8581 aagcggcaat cttggcatca aggccgctat ccaataaaac ctccaccccg gcaggcgtaa
     8641 cagcatctct ggatttgtcg aatttatccg tatatttgaa ttgttcctct ttgccggcat
     8701 cttcatcttc aggacaaccg aaaacataaa tataccgcac atccatacgt ttaacctttt
     8761 tcacgagcaa ctgaggatta ccccacttgc ccttatcatc caattggaag cggaacaaac
     8821 cttcctcacc ggcaatcctg ccgaccgcat aggcattgac cacatgcctt tgacgcgcta
     8881 tattgccatt ttgctcctta ccagaattct gaatcttcaa ttcttttttt gcatcttcta
     8941 aagtagggat ttgcttgccc ggtttcgata ttacgccaca gctgctgacg acggtagtct
     9001 cggcgcttgc attaacatca tcgattccgt attggaaaat caatgccgga cgaacctggg
     9061 caaaaccctg atatccgata tttaaagatt ccgctatggg gataagttta ttatctgtac
     9121 cgttggagat aactttattt gtactgttca tttttaagga aaaaagacgg tttttttgcg
     9181 ccacatcgga aacaacatcc ttttcggtat gctcagacat attgaaacaa ccgaagctcc
     9241 ccgccattct tgcatcgcgg acaatcaatg ttgccgcatt ccgcaaatcc tgttgaatgg
     9301 caagacgctc gtttgccgca tcatttaatt tccgggatgt aaagtaactc gatacgaccg
     9361 ctatcaggac aatcacactg agcaggcccg caaccagaaa ttcaacaatg gtaaaaccct
     9421 tcataccatc ataaccgccc tttggtacgt ttagcatttt acgtttcatt catgacctcc
     9481 gacccttgcc tgataggtat atacgatatt gtcgccgctc acctcaagat tcgtacggga
     9541 aatatccgaa tcccctgccg aatcatttac ccacaatact ttaatcaaag tatccccgtt
     9601 tgccttatta tcgcaatttg gagaaaaagt accgccggac aatgtcggcg cgccacccga
     9661 cgaatccttg cagacggcgt aatgaatagc taccgcatcc ggcaaggcgt ttctcaattc
     9721 ataatcgaac ctgtccaact gggcttttgc caaatccttc ttacttatca aattattatt
     9781 attaagcttg aaatcgccgc cagagggagt gggggtgtac ggccccgtgt aaagattata
     9841 gtttttcttg ttgctgtccg aatcaatggt cggattcatc aacattcctt ccatcaggtt
     9901 ttgcgtgatt tggctgacga tggtttgtgt ctccgcctcc ctgacggaag cgaccgtccg
     9961 caactgtacg gacagcaatg ccaaaatacc gatggtcaga acgagcatag cgaccaagac
    10021 ttctatcaac gccataccgg actggggatt tttcaggcgc aagcaatcat tattcttcat
    10081 tttttatttc ccaaaccgac taaaacagtc attaatgttt gcatacggcg cgcgtatcgt
    10141 ttttacgaca aacttcgacc ctgccgctgc tgttaatcaa aaccaccgcc gaacggaatt
    10201 tcttatcggc atctgaaact gctctcgcat ctgtcagcac gatttggata taaccgtcag
    10261 aataaacaaa tttggaatta ttcttgagat tctgatcggg caaatagccg aatgtcccgt
    10321 tttggttgaa agtccaaact acacggtcgg ctttcggctg agacgaaccg aaagcgatat
    10381 gattgaaggt gtaattaatc cggctgtcgt cggtatcatt caacaccacg ctgcggagga
    10441 aaacatccgt cgcatcgccg tcatatgcct tattgtcgtt tttgtcgccg aaagccaaca
    10501 ttccccaccc cttcttgctg aagtcacatc tattgttgga cgcaccgtct tttttaactt
    10561 gaacaggaca gatatagaca gggagattga gccggacggc ttcgcccctg gagaaacgca
    10621 aaaggttggc aacctgctcc gcgtgactgg caatgcggcg tgatgcaatc cacccactca
    10681 tattggggag ggctatcgtc gccataatgg ctgcgatggc catcacgatg agcagctctg
    10741 ttagcgtgaa accttgttgt tttcgtgtac acataagcaa taggacgtca actgtaatgt
    10801 attttcaatt atctgcaggc cggatggaag gcatcccgcc gcttttattt ttaggaaaac
    10861 cgcataggca attctaaacg attttacccg tttttgcct
//
LOCUS       DAWTHD010000079        10865 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWTHD010000079 DAWTHD010000000
VERSION     DAWTHD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.079"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            104..991
                     /gene="proB"
                     /locus_tag="WM704_001580"
     CDS             104..991
                     /gene="proB"
                     /locus_tag="WM704_001580"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFA9340966.1"
                     /translation="MKYKRIVFKVGTSSITRSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKAC"
     gene            991..1212
                     /locus_tag="WM704_001581"
     CDS             991..1212
                     /locus_tag="WM704_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HFA9340967.1"
                     /translation="MSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSAAAEDLL
                     KSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            1247..2488
                     /locus_tag="WM704_001582"
     CDS             1247..2488
                     /locus_tag="WM704_001582"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFA9340968.1"
                     /translation="MPLPKRQKKSVNTADAEEKNRALLAMADSLEAAAEDILAANRLD
                     LKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPNGLEIVKK
                     RVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAALKTGLAQT
                     RIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPVIETGTGI
                     VHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLERLLVRGRI
                     EAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVGHIEARGT
                     HHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQKLHARGP
                     IGLKELTSYKYIVQGTGQVRE"
     gene            complement(2664..5708)
                     /locus_tag="WM704_001583"
     CDS             complement(2664..5708)
                     /locus_tag="WM704_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFA9340969.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGAVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKTAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            5914..6594
                     /locus_tag="WM704_001584"
     CDS             5914..6594
                     /locus_tag="WM704_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340970.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTAALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNHYL
                     KSERGIWL"
     gene            6623..6982
                     /locus_tag="WM704_001585"
     CDS             6623..6982
                     /locus_tag="WM704_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFA9340971.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            7060..7166
                     /locus_tag="WM704_001586"
                     /pseudo
     CDS             7060..7166
                     /locus_tag="WM704_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7587..8123)
                     /locus_tag="WM704_001587"
     CDS             complement(7587..8123)
                     /locus_tag="WM704_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFA9340972.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(8157..8513)
                     /gene="folB"
                     /locus_tag="WM704_001588"
     CDS             complement(8157..8513)
                     /gene="folB"
                     /locus_tag="WM704_001588"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFA9340973.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            8569..9171
                     /gene="plsY"
                     /locus_tag="WM704_001589"
     CDS             8569..9171
                     /gene="plsY"
                     /locus_tag="WM704_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFA9340974.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            9263..10108
                     /locus_tag="WM704_001590"
     CDS             9263..10108
                     /locus_tag="WM704_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFA9340975.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(10217..10549)
                     /locus_tag="WM704_001591"
     CDS             complement(10217..10549)
                     /locus_tag="WM704_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340976.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(10599..10847)
                     /locus_tag="WM704_001592"
     CDS             complement(10599..10847)
                     /locus_tag="WM704_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340977.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
BASE COUNT         2488 a         3179 c         2673 g         2525 t
ORIGIN      
        1 ttattgcaga tgcggcgtga ttgttgacaa ttgcgacaga acagggcaaa ttcggcggta
       61 caaggcggca acgagacagg acaaaccaac aaggacggaa acaatgaaat acaaaagaat
      121 cgtatttaaa gtcggcacat cttcgattac ccgttcggac ggcagcctct cgcgcggcaa
      181 aatccaaacc atcacccgcc agcttgccgc attgcatcat gcgggacacg agctggtctt
      241 ggtgtcttcc ggcgcggttg ctgcagggtt cggcgcgctg ggtttcaaaa aacgtccggt
      301 caaaatcgcc gacaaacagg cttccgccgc cgtcgggcag gggctgctga tggaagaata
      361 tacggcaaac ctgtcttcag acggcatcgt gtccgcacaa atcctgctca gccgtgccga
      421 ctttgccgac aaacgccgct accaaaatgc cggcggcgca ctttccgtgc tgctgcaacg
      481 ccgcgcgatt cccatcatca atgaaaacga cacggtttcg gttgaggagt tgaaaatcgg
      541 cgacaacgac acattgagtg cgcaagtggc ggcgatgata caggcagacc tcttggtgct
      601 gctgaccgac atagacggtc tttacaccgg caacccgaac agcaatcccg atgccgtacg
      661 gctggacaaa atcgaacaca tcaaccatga aatcatcgaa atggcgggcg gctcgggttc
      721 ggcaaacggc acgggcggta tgctgaccaa aatcaaagcg gcaaccatcg ccgccgaatc
      781 cggcgtaccg gtgtatatct gttcctcact caaacccgat tcattggccg aagccgccga
      841 acatcaggcg gacggctcgt ttttcgtccc ccgtgccaaa ggtttgcgga cacagaagca
      901 atggctggcg ttctattccg aaagccgggg cagcgtttat gtggacgaag gtgcggaaca
      961 cgctttgtcc gaacaaggga aagcctgctg atgtcgggca ttgccggaat cgaagggcat
     1021 ttttcccgta tggacaccgt aaccgtgtac agcaaggcaa ccaaacagcc cctgggcaaa
     1081 gggcgcgtcc ttttcggctc cgccgccgcc gaagacctgc tcaaatcgcg taaggcgaaa
     1141 ggcgtgttca tccatcggga cgattggatt tccatcacgc ccgaaatacg cctgcttctg
     1201 accgaatttt agaaaaggaa acccatgtca aacacacaaa aacagcttgc ccttgccaaa
     1261 gcggcaaaaa aaatccgtca acacggcgga tgcggaagaa aaaaaccgcg cgctgcttgc
     1321 tatggcggac agtctggaag cggcggcgga agatattttg gcggcaaacc gtttggattt
     1381 gaaagcggcg gcaggcaaaa ttcccgacag catgaccgac cgccttttgt tggacggcaa
     1441 acgcatttgc gcgatggcgg acggcatcag ggcggttgcc gcgctgcccg accccgtggg
     1501 cgaaatactg gaaacctcga ctctgcccaa cggcttggaa atcgtcaaaa agcgcgtggc
     1561 gatgggcgtt atcggcatta tttacgaaag ccgcccgaac gttacttccg atgcggcggc
     1621 tttggcactg aaaagcggca gcgcggtcgt actccgcagc ggcaaagatg catttcaatc
     1681 cgcacgcgcc atcgttgccg ccctgaaaac ggggttggca caaacccgca tcgaccccga
     1741 agccgtacag ctcatcgaag acacagggcg tgagggcagt tacgaaatga tgagggcgaa
     1801 agattatcta gacctgctga ttccgcgcgg cggggcaggg ctgatacggg cggtggttga
     1861 aaatgccgtc gtgccggtca ttgaaacggg gacgggcatc gtccacattt atatcgacaa
     1921 ggacgcggat tgggacaagg cactccgtat cgtgtacaac gccaaaaccg gccgcccgtc
     1981 cgtgtgcaac tcgatggaag tgctgctggt gcatgaaggc attgctgccg acttcctgcc
     2041 caagctcgaa cggctgttgg ttcgcggccg tatagaagcc ggactgccgc ccgtccgctt
     2101 ccgtttggat ccgcaggcgg cgcggtatat cggcggcgaa gcggcgggtg cagacgattt
     2161 cgataccgag tttttagact acatcctcgc cgtgaaaacc gtcgcttcgg ttgaagaggc
     2221 ggtcgggcac atcgaagccc gcggcacgca ccattcagac ggcatcgtta ccgaaaaccg
     2281 ccacgctgcg gactatttca cgacccatat cgattctgcc gccgtgtata tcaacgcgtc
     2341 cacgcgcttc accgacggcg gcgaattcgg cttgggttgc gaaatgggca tctccacgca
     2401 aaaactccac gcccggggcc cgataggttt gaaagagctg acgagttaca aatacatcgt
     2461 acaaggcacg ggacaggtta gggaataatc cgaattcaaa cgtctgatat agtggattaa
     2521 atttaaaccg gtacggcgtt gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg
     2581 ccgccttatc ctgatttttg ttaatccact ataaaaatgc cgtctgaaac agtcttcaga
     2641 cggcattttt atttgcaaaa aaatcaagca ttgtccaagg ggacgagaat cgtacggttg
     2701 ccgttgtgtt ccggtgcgga cacaatgcct tccgcttcca tttggtcaat cagacgcgcg
     2761 gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg aaatgctggc tttgcgcgtt
     2821 ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg tttcgccgtc gccgctgcgc
     2881 ccgataccgg gcagctcttc gctgccgccg ccgctcaaaa tatcgtcaac atagtccggc
     2941 tcgccaaact gcttcagata ttcgaccacg cggtgcacct cttcatccga ggcaaacgcg
     3001 ccgtgaacgc gctgcggata ggcagtaccc ggcggcagga acagcatatc gccctgaccg
     3061 agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc tgtcgatttt gctggacact
     3121 tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac ccgtgatgac atcgacgctg
     3181 gggcgttgtg tggcaaggat aaggtggatg cctgccgcgc gggctttttg ggcgaggcgc
     3241 gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca aatcggcaaa ctcatcgacc
     3301 acgaccacga taaacggcag tttttccaaa ggttcgggat cgtcgggcgt gaggctgaac
     3361 ggattgccga ttttttctcc ccttgctgcg gcttcggcgg ttttttggtt gaagcccgca
     3421 agattgcgca cgcccataaa gctcatcagg cggtagcgtt tttccatttc gttaacacac
     3481 cagttcagcg cgtttgccgc cagcttcata tcagtaacga caggggcgag caggtgcgtg
     3541 atgccttcgt aaatgctcaa ttccagcatt ttcggatcga tcataatcat acgcacgtct
     3601 tccggcgcgg ctttgaaaag catagacaga atcatcgcgt tgacacccac cgatttgccc
     3661 gaaccggtcg tgccggcaac cagcaaatgc ggtgctttgc ccaagtcggt tacgacgggc
     3721 tgtccggtaa tgtcctgacc gagcgcgagc gtcagcttgg atttggattc ggcaaactcg
     3781 ggcgaattga aaatttcgct caggcgtatc atttggcgtt tcgggttcgg aagttccaaa
     3841 cccatgcagg ttttgccggg gatggtttcg acaacgcgga tggaagccac gccgagcgaa
     3901 cgcgccaagt ctttttccaa attcagaacg gaattgccgc gcacgccgac atcgggttcg
     3961 atttcataac gcgtaatcac ggggccggaa taagaatcga caaccttgac cttgactttg
     4021 aactccgcca atttttcttc gatggtgatg ctgttttcca acagttcttc ttcggtttgc
     4081 gtcgcctcgg gattgaacag cggaggcaga agcaggtcgg ttgtcggcag gtgttcggat
     4141 accgcaccgg tctcttccga ttggaacgcc ccttcatccg cttccgtatc cgatacccgg
     4201 caggacgggc gttcagacgg cacatcttca aacggacaaa ccgcctgact gtcatgcccg
     4261 aacgcttcgg tttccgacag atattgcccg cttgaccgct ctgccgcacc ctcactgccg
     4321 tcatctgcaa tagcggcggt ttcctcctgc caacctccat tcaaaacatc agcggcaaga
     4381 tggtcggttt cggcaatgcg gctgcgttgc gcctgctcga atcctgccgg cggctcatag
     4441 gtacggttgt agatttccga tacgggaggc ggcggcagaa tatcgatttc gggtacggct
     4501 acctccggca cttcgggtgg ttcgaccacc caagcatcgg gcgcggcagg ctcttcaata
     4561 tggatatcgg cagcttcatc ataaaccgga ccgccgtatc ccacagacga aacggtttcc
     4621 gtgaaaacat tttcggtttc agacggcatt tcgacatccc gtttcccgac aaccgtccga
     4681 ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa ccgttgcctt gttcggttct
     4741 ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg ctgtgccgtc tgaaacggac
     4801 tgcccttggg aaacatccgg ggattttgcc tccgcctgcc ctttctcctg ccctgcatga
     4861 cggcggaacg gcgcattccg tgcattttcg gctttgactg cagaaaaggc agacggatgc
     4921 tgcttctcaa aatacggctc aaatccgtaa tccgcagact cggaaaacgc ttctttgtcc
     4981 gcatcaaaca tacgggaata cgtgcgttga agcacagggt cgtccaaacc gataatctgc
     5041 agcccttcca tcggggtatc ggacacgcgt actttgggga cggctgttcc gtttttctca
     5101 aatgcatcga tatagcgttt tttcgtttcc cttaaagccg cgctgcttaa agcacgcgtt
     5161 gcttcttcca aagtgatttc cttaaaaacc ggacggacgg gcgaagtttt gctttcagac
     5221 tgcatcaacc cttcagcaat actccggtcg aatgggatgc ggcggttgtc gattacggca
     5281 gttgcaatgt cttccgtatc ggcagcctct tcctccgcag cttctgctgc ttccgtttcc
     5341 gcttcttccg tcccgtttcc actgtctgaa tacccgtcgg atgcggtcgg aacatccgca
     5401 cggtttgcag aagcaggcgg aacgggcctg tccggttcgg attcgtgcgc cagatagttt
     5461 cggcagaaac ggacggcacc gacaaacagc caatacactg ccgttttgac ggcatggaaa
     5521 agcatcaggg aaaactcggg aaaatcgggc ataccgtctt tgatttcagg cagctccgcc
     5581 tgtttttccc ctttcttttc ctgccacgca gaaacctcgc gcatccactc gcgttcggat
     5641 tgtgcgcgga caaaaaacag gccggcaagc gcaagcaaca aaataacgat caaaactatc
     5701 caaaacatat gccgtttccc gcaagacaaa cgattaaccg agtttatttt aacgtgtttc
     5761 cagcccaaaa aaaacaaccg gccggcaatt ctgccgaaac cgattcggct ttcggtttgc
     5821 ccccgccgac agtataatag ccgccccttc ccttaatctt tgcggcatta ccatgatttt
     5881 ccaaacagtc tggttttcat ttgcggcatt accatgattt tccaaacagt ctggttttca
     5941 gatgtggtac tgtcggtttc gtggattgtc cttatcctga tactggcagc ttccgcgccg
     6001 tcggcattcc gttcgcttgc ccgctaccgt tccgcccttc ccctgtgtac cgtaattttt
     6061 tccgcagcat ggtgtctgaa cgcctctgtc ggcggcggac agcttgccca gatgaactac
     6121 cacctgcttg ccgtcaatct ggttacactg atggtggata cctccgccgc cctttggctt
     6181 gccgcgctgc tgatgctgcc ctactgcctg ctgtttgccg gttctgccgg cgcatatccg
     6241 ccgaacgctt tggtgctgat actgcccgcg cttgtcgtca accgcctgtc gcgtatgctg
     6301 gtcaaccgtc tgccgcccaa tattttcatc tttattttcg tcaacggctt tctcgcttcc
     6361 gccgccggca ttctgctgac cgggctggtg ctgatcggta ttttggatgc cgcaaacgct
     6421 tttccgtccg aaatattgtg gacggccgcc ctgcccgtct ttattctgct ggcgtgggcg
     6481 gaagccttcc tcagcggcat ttcaactgcc attttcgtgg cactgaagcc ccattggatc
     6541 aacactttcg acgacaacca ctatctgaag tccgagcgcg gcatatggct gtagccttca
     6601 gacggcatag gcagacaaaa ccatgctttc caacatcctc cccctttcca tcggtgcaat
     6661 attcggcaca acggcgcgtt ggctgctcaa cctcgccgtc cccgcatcgt tgtctcccgc
     6721 cacaggcaac ctgtttgcca actggacggg cgcgctgctc atcggcatct ttgccgaaac
     6781 cgtcagccat ccgcaatgga agctgctgct gattaccggt tttttcggca gcctgaccac
     6841 gctttccgga ttttcactgg aaaccgtaac cctgctccaa tcgaaccgtc ccgcttcggc
     6901 acttgccaat attttcctac acacggcagg ttcgctgctg ctgacttggc tcgggctgaa
     6961 gatagggacg gcagtcaaat aaccgcaaaa aattgcttcc tttggttttg acataatcta
     7021 taaattggct tcgcttgttt aaatactgcc tgtgccaatt atagtggatt aaatttaaat
     7081 caggacaagg cggcgagccg cagacagtac agatagtacg gcaaggcgag gcaacgccgt
     7141 accggtttaa atttaatcca ctataccgtt ctcaaattca aattgggctt ttaggtctat
     7201 aacaaagaaa aagccttggg aggataaacc taccaaggct ttttcttaaa aaacgctcta
     7261 tcactgtgcc taaaaatgca aaaaagtaac cgtcataatc cttttgccaa aatcaatatc
     7321 atcatccgat gaatgaacgg acggtatata agaaagcgtt aataaactca taaaccagta
     7381 agaaaaatca gtaacgaaga tattcattat tacccattct taaaaattat cacgttcgtt
     7441 tgggtaatta tcataccctt ttccctctta ttactaaagc acgaattact ttaaagtacg
     7501 cacccagaga caagccgaaa aggcatatcc gatccgccaa acaaaatgcc cgccgactta
     7561 ctccggcggg cggttccaac atcctgtcaa tccttcatca gccagtattg caaaccgatt
     7621 aatgttttac cgtctttaat ttcatcgttt gccaatgcct gacggacttc atctttcgac
     7681 atcagcacgg tttccgttat ttcatcttca tcattagaaa gcgtactgcc caagcgcacg
     7741 ccttccgcct cgaacagata cattttttca ttgcaaaaac cgaccgccgt ataaaaactg
     7801 tacagcaggc gcacgctgtc ggcaacataa ggcgtttcct ccgccaattc tcgcagcgca
     7861 cacgctgcca tatcctcgcc cgccacatcc agcttgcccg caggaagttc caatgtcgcc
     7921 tgatttgccg cataacgcca ctgccgcacc aaaacgacct tttcgtcatc cgttaccgcc
     7981 aacacgcacg ccgcgcccgg atgccggatg acgatgcgct gcccttcatt gccgttgggc
     8041 agcctgacct tatccctgct gatactgacg aaaccgccct cgtaaatggt ttcgccgcct
     8101 aattttactt ccctcaaatc cacttcatat ctcctttccg tttcctattc tgccgttcaa
     8161 tcttcacgct tgccgcgctc gatttccact ccaacctcgc gcacgccttc caaaataccc
     8221 ggcttgacga ttttcacgtg cacccacacc gcgccgaaat atcccaaaac caaatcggca
     8281 atatattccg ccaacgcttc caaaagcagg aaatcctgtt ctttcagatg tcggcgcagc
     8341 gtttcgcata cctcggcata atgcaccgta ttggcaatat cgtcgtccga acccgctttc
     8401 tcgggaacgc cgatgtccaa atcgacaatc agggtctgca accgttcgcg ttcccagccg
     8461 tacacgccga taagcgtatc cgccttcatg ccgtgcaaaa agattttatc cataatccgc
     8521 cccgtttttt gctaaaataa ccgcaccatt ctaagtaaag taccgcaaat gttcaacata
     8581 ccggccgtcg ccgtttccta tttaatcggt tcactttctt tcgccgtcat cgtttccaag
     8641 tattacggga tggacgaccc gcgcacctac ggatcgggca atcccggcgc gaccaatgtt
     8701 ttacgcagcg gcaaaaaaaa ggcggccgcg ctgacgctct tgggcgatgc cgccaaaggt
     8761 ttggttgccg ttttgcttgc acgcgtgctt caagaaccgc tcggtttatc cgacagcgca
     8821 atcgccgccg tcgcactcgc cgcgctggtc gggcatatgt ggccggtgtt tttcggattt
     8881 aagggcggca aaggcgtggc aacggcattg ggcgtgcttc tggcactctc tcctgcaact
     8941 gccttggtct gcgcgttgat ttggcttgtg atggcattcg gcttcaaagt atcctccctt
     9001 gccgcgctgg tcgccacaac cgccgccccc cttgccgcac tgttttttat gccgcatact
     9061 tcttggattt tcgcaaccct cgcaatcgcc atattggtgt tgctccgcca taagagcaac
     9121 atcctcaacc tgattaaagg caaagaaagc aaaatcggcg aaaaacgctg attcggcatt
     9181 tgttttccat tacgtcatgc cgtctgaaac catgttcccg cgttcagacg gcatttccgc
     9241 acaaacggct ttcatacata ccatgctcag ttacagacac gcattccacg ccggcaacca
     9301 cgccgatatg ctcaaacact tcaccttgtt tttagtgctg caatatttca accgcaaaga
     9361 caagccctac tggtacatcg acacgcacgg cggcgcgggt ttgtacaatc tcgaaggcag
     9421 cgaggcgcag aaagtcggcg aataccggca aggcatcgcc ctgctccgac aggcgcaaaa
     9481 cctgcctgcc gaactctctg actttgccgc gcacatacaa aaaatcctac cctcgcccga
     9541 actttactgc ggctctccgt ggctggcgca atcgctgatc cgcatcggcg acaaattgcg
     9601 cctgtttgag ctgcacccca ccgactttgt ccatctgcaa aacaatatgg gcgaagcggg
     9661 attgggcaaa cgcggacaag tgttgcgcga agacggttac aaagggctga tttccctgct
     9721 gccgcccccg ccgcgccgcg ccgccgtcct catcgacccg ccctatgagg aaaaacagga
     9781 ctaccggcgt gtaacggaaa cgctgaaagc cgctttaaaa cggtttgagt ccggctgtta
     9841 tctcatttgg tatccctgcc tcagccgcga agaaagccgc aagctgccgg aagaattgaa
     9901 aaaactcctg cccgacaact acctttacgc cgaactgcac gtccacgcgc cgaaaaccga
     9961 cggtttcggt atgcacggca gcggaatgtt cgtgattaat cccccttacc ttttagccga
    10021 acaattggcg gcaaacctcc ccgccctgac acggctgctg gcgcaagacg aaggcgcgcg
    10081 ctacctgttg gacaacaaaa tccgctgacc ccgatgccgt ctgaaaccct tcagacggca
    10141 tcttccgtca aacaaacaaa accgggcgat acacactcca tatcgtccgg ttctgcataa
    10201 aaccagtatt caaaaatcag aacacgcttt tcattacacc cacggtacca accacaccgt
    10261 tcagcagcat cagaccaccg gcagccgtac ccataccttg acggattgcg tcatgcttat
    10321 tggcacgttt ttcacgttcc aaagccaact cctcagaaac atttttacgt tgacggcggt
    10381 gttgcgccag ttccgcacgg ctaattcgcg cttcggcacg gttacgacca gccttgatta
    10441 cggccgaatc gtcgccgata gcatagttgg ctacagaatt ttcagaagcg caggcagcaa
    10501 gcaaaacggc acacacagca acaacggaaa tatttttcaa gtttttcata cctgtcctat
    10561 tatctcaaaa taaagaaaaa actaatccaa ctggagtttt atcggataaa ataaatattg
    10621 ggtgaatttt cgtttgcctt tttgatggcg cgtgccgcat ccatcatttc ttcccggcgt
    10681 tctttacggt attcggcacg ctcggcacgc tcgcttatct gaatggcacg acttttcgcc
    10741 tgtatttccg ctgcagattc ttgagttaca aaacggcggt ctgcgtaagt acatgccgtc
    10801 accaacatcg cagccaccga caacaaaatc agtttcttca ttttcatcta cctatattta
    10861 agttt
//
LOCUS       DAWTHD010000080        10790 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWTHD010000080 DAWTHD010000000
VERSION     DAWTHD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.080"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..201
                     /locus_tag="WM704_001593"
     CDS             <1..201
                     /locus_tag="WM704_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFA9340978.1"
                     /translation="YEYLVLLFIMTSKNIACDSDIKRRAYHLRFMLLKSNKLIYKLIF
                     PIITLFGVDITGRILKAIRGNI"
     gene            242..772
                     /locus_tag="WM704_001594"
     CDS             242..772
                     /locus_tag="WM704_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFA9340979.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(940..2535)
                     /locus_tag="WM704_001595"
     CDS             complement(940..2535)
                     /locus_tag="WM704_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFA9340980.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFGIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(2642..3037)
                     /gene="hisI"
                     /locus_tag="WM704_001596"
     CDS             complement(2642..3037)
                     /gene="hisI"
                     /locus_tag="WM704_001596"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFA9340981.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3068..3835)
                     /gene="hisF"
                     /locus_tag="WM704_001597"
     CDS             complement(3068..3835)
                     /gene="hisF"
                     /locus_tag="WM704_001597"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFA9340982.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(3848..4585)
                     /gene="hisA"
                     /locus_tag="WM704_001598"
     CDS             complement(3848..4585)
                     /gene="hisA"
                     /locus_tag="WM704_001598"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFA9340983.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(4618..5256)
                     /gene="hisH"
                     /locus_tag="WM704_001599"
     CDS             complement(4618..5256)
                     /gene="hisH"
                     /locus_tag="WM704_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFA9340984.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5386..6888)
                     /gene="pta"
                     /locus_tag="WM704_001600"
     CDS             complement(5386..6888)
                     /gene="pta"
                     /locus_tag="WM704_001600"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFA9340985.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7073..8131)
                     /gene="fbpC"
                     /locus_tag="WM704_001601"
     CDS             complement(7073..8131)
                     /gene="fbpC"
                     /locus_tag="WM704_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFA9340986.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8152..9669)
                     /gene="fbpB"
                     /locus_tag="WM704_001602"
     CDS             complement(8152..9669)
                     /gene="fbpB"
                     /locus_tag="WM704_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFA9340987.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(9737..10732)
                     /gene="fbpA"
                     /locus_tag="WM704_001603"
     CDS             complement(9737..10732)
                     /gene="fbpA"
                     /locus_tag="WM704_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFA9340988.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
BASE COUNT         2198 a         2645 c         3379 g         2568 t
ORIGIN      
        1 tacgaatatc tcgttttgct ttttataatg acgagtaaaa atatagcgtg tgattctgat
       61 ataaaaagaa gggcatatca tttaaggttt atgctgttaa agtccaataa gctgatatat
      121 aagctgatat tcccgataat cacattattc ggggtcgata ttacaggcag gattttaaaa
      181 gcaatcaggg ggaatattta ataaatcctt taacaatata taccttaccg aaggaggaaa
      241 aatgaacgca atcagaactt tccaaaaccg catgcccgaa atccacgaaa cctgcatgat
      301 agacgaggcg tgcgtcgtca tcggcgaagt ctcgcttgcc gaagatgttt ccgtgtggcc
      361 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
      421 ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag ggtcgccgct
      481 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgaatcgg
      541 taaccgcgtc ttggtcggta tgggcagcac cgttttggac gatgccgtta ttgaagatga
      601 cgtgatgatc ggcgcgggca gcctcgttcc gccgcgcaaa cgtttggagg gcggctatct
      661 ttatatcggt tcgcctgtga ggcaggttcg tctgctgacc gatgaggaaa aagccttttt
      721 gaaatattcc gccgcacatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
      781 cggcgttctt gcgtcagtcc caaattcacg cggttgggat gcccgataac ggtatccgat
      841 gcgccttgat tttgaccggc tgcgtttgag ttgcaggcaa aaatgccgtc tgaaagcctt
      901 tttttcggct tcggacggca ttttattgcc gatttctttt tagagtttga ccgaatgttc
      961 gcgcgtttcg tggaacacga tgtccggcca acgttcttgc gtcaacccca aattcacgcg
     1021 gttgggggcg aggtaggcga ggttgccgcc tgcgtcgatg gcgaggttgc ctgcgttggc
     1081 tttttcaaat tccgccagtt ttttcttgtc gtcgcacgat acccagcgcg ccgaccagat
     1141 ggatgcgctg tcgaacacgg cttccacacc gtattcgttg gcgaggcgtg aggttacgac
     1201 ttcaaactgc aacacgccga ccgcacccaa aatcaaatcc gcgccgctca tcggtttgaa
     1261 tacttgaacc gcaccttctt cgccgagttg ttgcaaacct ttttgcagtt gtttgatttt
     1321 cagcgggttt ttgatgcgga cgctgcggaa cagttcgggt gcgaagaatg ggatgccggt
     1381 aaacgccagt tgttcgcctt cggagaagct gtcgccgatt tggatgttgc cgtggttcgg
     1441 gatgccgatg atgtcgccgg cgtaggcttc ttccgccagt tcgcggttgt gcgacatgaa
     1501 ggttactacg ctggaggcgg cgatttcgcg gttgatacgc aggtgtttca tcttcatgcc
     1561 gcgctcgaat ttaccggagc agacgcgcaa gaaggcgata cggtcgcggt gtttcgggtc
     1621 catattggct tggattttaa agataaatcc ggaaaatttc ggctcgtccg gcccgaccat
     1681 gcgcatggtc gcgtcgcgcg gtttcggtgc gggtgcccag tcaatcaatg aattgaggat
     1741 ttcctgaatg ccgaagttgt taatcgcaga gccgaagaac actggcgtga gttcgccggc
     1801 gagaaattcg tcgagattaa attcgttgga agccgcctgc accaattcga tttcgtcgcg
     1861 caactgctgg atttccaacg gaaagcgttg ttccaattcg ggattgttta tgcctttgat
     1921 gatgccgaac tcgtgcggca ggcgttcgcc gcccgcttca aagagataga tttcgtcgtt
     1981 caggatgtgg tacacgccct tgaagttttt gcccataccg atcggccagg taacgggcgc
     2041 gcagcggatt tgcaggatgt cttccacttc gtccaagagt tccaaagaat cgcgcacttc
     2101 gcggtcgtat ttgttcatga aggtaacaat cggcgtatcg cgcaggcggc agacgttcaa
     2161 gagtttgatg gtttgcgctt ccacgccttt tgccgcgtcg atgaccatca aggcgctgtc
     2221 cactgcggtt aaaacgcggt aggtgtcttc ggagaagtct tggtgtcccg gcgtgtccaa
     2281 gaggttgacg gtgtggtctt tgtagtcgaa ctgcatcacg cttgatgcca cggaaatgcc
     2341 gcgctgcttc tcgatgtcca tccagtcgga ggtggcgaat ttgccggttt tcttaccttt
     2401 caccgtgcct gcgctttgaa tcgcgcccga aaacagcagc agtttttcgg tcagcgtggt
     2461 tttacccgca tcggggtggg agatgatggc aaacgtgcgg cggcggcgca cttggtcgag
     2521 gatttcttgg gacatggttt tctttgcaaa aaggttcagg ccgcttttca gacggcccgg
     2581 acagtgtttg agacggcgaa attgtacaaa aaaatgcctg ataattcaat gttggaggcg
     2641 gtcagtgcgt gctgccgtaa atctcttttt cgtctttcag gacggcatcg gcggtttccc
     2701 acgcaccgcc gttccatttt ttgtaaaagc agctttcgcg cccggtgtgg caggcgatgc
     2761 cgccgttttg ggcgatgagc atcacaatgg tatcgccgtc gcagtcgagg cgcagttcgc
     2821 ggactttttg cgtgtgtccc gactcttcgc ccttcatcca ttgtttttgg cgcgaacggc
     2881 tgtaatagtg ggcaaagccg gtttcgacgg ttttttgcag ggcttcggcg ttcatccacg
     2941 ccaccattaa aacacgtttg gtttcggcat cttgggcgat ggcgcaaacc aaaccttttt
     3001 cgtcaaattt gacggcttca agcaggtttt tatccatatt tcctttcaga cggcatagtc
     3061 ggggcggtca gaggcgcact tcgatgccgg cttcgcgcat agtgcgtttg gcttcgcgga
     3121 tggcgatttc cccgaaatgg aaaatgccgg cggcaagtac ggcatcggct ttgccttcgg
     3181 ttatgccttc aatcaggtgc cggacattgc cgaccccgcc ggaggcgatg acggggatgt
     3241 cgacggcttc ggcaacggcg cgggtcagcg gcaggttgaa accctgtttc gtcccgtccc
     3301 tgtccatacc ggtgagcagg atttcgcccg cgccgcgttt ttgcatttcg accgcccatt
     3361 ccaccgcatc caaaccggtc ggattgcgcc cgccgtgggt aaagatttcc cagcgtgtgt
     3421 tttcggggtt gacggctttg gcatcgacgg cggcgacgat ggcttgcgaa ccgaaaaatc
     3481 cggcggcttc gttaattaaa tcgggacggg taacggcggc ggtgttgatg ctgactttgt
     3541 ccgcgccggc attgagcagg cggcggatgt cggcaacggt gcgtacgccg ccgccgacgg
     3601 tcagcgggat gaagacttgt ccggcaacct cttcgatgat gtgcaggatg gtgtcgcggt
     3661 tgtcggatga ggcggtgatg tcgaggaagg tcaattcgtc cgcgccttcg ccgttgtagc
     3721 gtttggcggc ttcgacgggg tcgcccgcgt cgcgcaaacc gatgaagttc acgcctttga
     3781 cgacgcgccc gtcttttacg tcgagacagg ggatgatgcg ttttgccagt gccataatcg
     3841 gatgccttta gtcgagggaa tctgccagtt gctgcgcttg ggcaaaatcg atgctgccct
     3901 cgtaaatcgc gcggccggta atcgcgcctg ctacgccatg tttttcggcg gcacacaggg
     3961 cggggatgtc gtccaagccg gtcagtccgc cggaggcgat gacgggaatg cggacggatt
     4021 gggcgagttt gaccgtcgcg tcgatgttca cgccgctcat cataccgtcg cgcccgatgt
     4081 cggtgtagat gatgctgttg acaccgtcgt cttcaaagcg ttttgccaaa tcaattacat
     4141 gatgcccggt tacggttgcc cagccgtcga tggcggccat accgtctttg gcatccagtc
     4201 cgacgataat cctgccgggg aaggctttgc acgcttcgcg caccaagtcg gggtttttga
     4261 ctgccgccgt gccgataatc acgtcgttca gtcccaaatc caaatattgt ccgatggttt
     4321 tcaaatcgcg catgccgccg ccgagctgca cggggatgtc tttggcgacg gcggcgagga
     4381 tgtctttgat ggcgggcagg ttttgcggaa cgccggcaaa tgcgccgttc aaatctacca
     4441 ggtgcaggcg gcgcgcgcct tgttcgaacc agtgcagcgc ggtgtcggcg ggcgaatcgg
     4501 aaaagacggt cgcctcttcc atcagccctt gtttcaagcg gacgcaacgt ccttctttca
     4561 aatcgatggc gggtatcagc agcatagttt ttctccttgt gcggggccgt gtcgggctta
     4621 ccagtttaaa aagtttttca acatcgtcag cccggcatcg tggctttttt cggtgtgaaa
     4681 ttgcgtggcg aatacgttgt ctttgccgac gatgcaggca aacggggacg ggtagtcgct
     4741 ttcgcccaat atggtttcgg gattttcggg ggcgaaatag tagctgtgga cgaaataaaa
     4801 acgcgtgttt tgggggatgc cttgaaaaag cgggtggttt tgggtttggc gcacggtgtt
     4861 ccagcccata tgcgggactt tcagacggca tccctgcggg tcgcggaggt cgcgggcaaa
     4921 gcgtctgact ttgccgccga accagcccaa gccgtcggtg tttccttctt cgctgtggtc
     4981 gaataaaagt tgcgcaccga cgcagattcc gaaaaacggt ttgtttttta aggcatcttt
     5041 gactgcctcg tccaagccgc cgcgcgtcag tgccgccata cagtcgggca tcgcgccctg
     5101 cccgggaaaa atgactttgt cggcgcggga cacgcggtcg gggtcgccgc ttaaaaagat
     5161 tttggtattt tttccggcaa gctgccccgc cgtccggacg gatttcaata cggaatgcag
     5221 gttgcccata ccgtaatcga taatggcggt ttgcatggct tcctcctctt ttttgcaata
     5281 tggctgcgat tttaacaaac aaatgtgccg ggctgataaa aatgccgtct gaaaacggga
     5341 atctgtcttc agacggcata gggttcaaac ccggaaagcc gtttgtcagc cttccatttg
     5401 ttttgcctga acggcagtca gggcgatggt gaacacgatg tcttccacca gcgcgccgcg
     5461 agacaggtcg ttgaccggtt tgcgcaggcc ttgcagcagc gggccgacgc tcaagacgtt
     5521 ggcactgcgt tggacggctt tataggtgca gttgccggtg ttcaggtcgg ggaagaccaa
     5581 aacggttgcc tgtcctgcca ccgggctgcc cggggctttg gatttgccta cgcccggcac
     5641 ggttgccgcg tcatattgca gcgggccgtc gatggcgagg tcggggcgtt tttcccgggc
     5701 aagtttggtg gcttcgatga cggtatcgac atcggggccg ctgccggagt tgacggtgga
     5761 gtaggaaatc atcgccactt tcgggtcgat gccgaaggct tttgcggaat cggcagactg
     5821 gatggcgatg tcggcaagct gttgcgcggt cgggttcgga ttaaccgcgc agtcgccgaa
     5881 caccagaacc tgatttggta gcagcataaa gaatacgctg gatacgaggc ttgcgcccgg
     5941 tgcggtttta atcagttgca aagcggggcg gatggtgttg gcggtggtgt gaaccgcacc
     6001 ggataccaaa ccgtccacat cattttgcgc catcatcatc gtaccgagta ccacggtgtc
     6061 ttgcagttgc ttgcgcgcgt cttcgggtgt caggcctttg gatttgcgca gttcgcacat
     6121 cggctcgacg tattgttcga ccaatgaggc gggatcgatg atttccaaag agtcgggcag
     6181 gctgatgccg cgttctttgg caacggcttc gacttcttcg cgtttggcaa gcaggacgca
     6241 gcgggcaatg cctttttcgt ggcagatggc ggcggcttgg acggtgcggg gttctgcgcc
     6301 ttcaggcagg acgatgcgtt tgccggcttg gcgggcgaag tcgatcaggt tgtagcggaa
     6361 ttgcgccggc gacaggcgtt ttgcttcgcg gcctgccaat acggatacgt ctttcagcgc
     6421 gtcgctcgaa ccgaagaagg tcaggccggt tttctcggct gccgcttcgg caacggaggc
     6481 tgccgcaccg tccacgacaa aaccttccaa tacgcccggc gcggcggcga agaactgttt
     6541 ggcaaggttc aattggtgtg ccagcgcgtc ggcatcggta ttgtcggaac ggacggcgaa
     6601 gacggctgcc gcgtcaaggg acaatgccag ttcgacgttt ttgcctgcga gatagatttt
     6661 gtcggcatcg ggcgcgatgc cttcgatgac gaggttggcg gcatcgagtg cggcaacttt
     6721 gccgaccagt gcgtcgaacc agtcgtcgct tttgccttgc gcgagcaggg tttcggcggt
     6781 tgcgtcaacg gcttggaaaa cttgtgcgtc cagtgctttt gcaaaggctt gtgcggcggc
     6841 ggaggtgttc agtccggcag atacgggtac gacgagtact tttgccataa tatatccttt
     6901 cgtatgctgc ggtgtgcggc atatgtggtt ggaaggggcg gcatatgggc agaaacggct
     6961 gcctgcgtgc cgtgcgtgcc gtgtttggct tggggcgcgc aggttgaata tagcaaacaa
     7021 attctgtttc caacaagata aatatccgca ggcttgtgga tgctgccgcc tttcagaggg
     7081 tatttccggg gaagaacagg gcgggaccgt ccaaatggag gacggcggaa atgccgtctg
     7141 acagggtggg ggcggaaggg aggttgagcg tgaggacggt ttgtccggcc ctgaggctga
     7201 tttcggtatg ccgcgctttg ggcgtggttt tgagaaccac ggcgtgaatg gaggcggcgg
     7261 gtgcggaatg ggggtgaagg ctgaactgtt ccggacggat gagcagtgta ccgcgcgtgc
     7321 ctgcgggtgc gccgctttgg acgggcaggc ggcccaatct gcaatcggcg gtgccgtcgg
     7381 cgttgagcgc ggcggggaac acgatgcctt cgccgataaa cagggcggca tcaaggtcgg
     7441 caggttgtcg gtacaattcg tgagggcttg cggtttggag gatgcgcccc tgtttcatca
     7501 cggcaatccg gtcggcgtat tgcagggctt cttcgcggtc gtggctgaca aaaacggcgg
     7561 attttccgtt ggcgcgcagg gcggcaatca tgtcttcgcg aatctggcgg cgcaactgtt
     7621 cgtccagcgc gctgaagggt tcgtccaaca aaatcagttc ggggtcgggg gcgagggcgc
     7681 gggcgagggc gacgcgctgt tgttgtccgc ccgaaagttc gtgcggatag cgtccggcaa
     7741 gttcggaaat gccggtcaat tccaacatgg cttcgatgcg ctgtcgctct tgcgccgtcc
     7801 tgcctttgcc gttgccgaga ccgtaggcga tattgcggta aacggtcagg tgggggaaca
     7861 gcacgccttc ctgtacgagg taacccaaac ggcgttcgcg gacgggaagg ttggtatttt
     7921 tcgagaagat ggttttgccg gaaagcgaaa tttcgccgga atcgggttgt tcgaaaccgg
     7981 caaggcagcg taaaagggtg gttttgccgc agccggacgc gccgatgata aagagaattt
     8041 cgcccgggtc gaggctgagc gaaatgtcgt ttaaaaccgg ggtgttttga aaacttttgg
     8101 acaggtgtcc gatgtgcagg gcggcggtca tagcggtgct tcctcaagct gttatttgaa
     8161 ggcgtatttc ttcagcagga atacgggtat gccggaaaat aataccagca tcagcgcgta
     8221 aggggcggcg gcggcgtatt gtgcgtccga tgtgtattcc caaacggcgg tggagagcgt
     8281 gtggacatcg tcggcggtca gcagcagggt ggcggtcaac tctttcatca gcttgaggaa
     8341 gacgagtgcg aatgcggcgg taatgccggg caggatggac ggcagtacca acgtcctgaa
     8401 aataaagaag tgtccgcgcc ccaatgttgc gccgacctgt tccatccctt tggggagttg
     8461 ttccaaggaa gtcctcaggg tggtttgcgc catcggcagg taaagcatga aataggcaag
     8521 gatgacgacg ataaaggttt ggtaaacggc aggggtgtag ttgatgctga aataaaccaa
     8581 ggatagggcg ataaccaaac cggggacggc gtgcagtaaa aacggcagcc tgtctatcca
     8641 aacggttaaa aaattgcgat agcgaaccga tgcccaaaca aggggcaagg cacataatat
     8701 agtcaaaatc gcacctaaag ccgatacgct taaggaacgg ataaaggcat caaatacgga
     8761 tacgagcgcg aatgtgccgg aagtgccgac catcatccaa tgtatcaata cgccaaaggg
     8821 gataataatg cccaaaatca acaagctgct taaaaaaaca atcgcgccga tctgaccggg
     8881 cagtttgagg gttttgacgg gataaggacg ggcaacgcct ttgccgctgt ggtaaatctt
     8941 ggctttgccg cgaaatatgc tttctccaaa tacgacgatg ccgcacaccg ccgtcaaaac
     9001 agcggaaagc agggcggcgg tattgttgtt gtaggacatt tcgtattctt ggaaaatggc
     9061 agtggtaaaa gtggggtagt tcaaaatgga taccgcgcca aattcgacca gcatatgcag
     9121 ggcgatcagt aacacgctgc tgccgatggc gggtttgagc tgcgggagga tggcggaaaa
     9181 aaaggtttgc aggcggcttt tgcccaagga caggctgact tcttcgtaag acaggctgat
     9241 gcgtttgagt gccgcctcga cgggcaggta ggcgagcgga aacgaggaca ggctcataat
     9301 catcactgtc ccccaaaagc cttcgacgcg gaaggtcagg ctgatccagg tgaaacagct
     9361 gacaaatgcg gggatgcaca aaggcagggt gattgccgtc tgaaaaaagg ttttgccgaa
     9421 gaagcggtaa cgttggaaca aaagggcgca ggcaatgccc aaaacaatgg aaatcagggt
     9481 aacgcccgcc atcatcgtca aggtgttgga gagcaaatcc cacatacgcg ggcggaacaa
     9541 cagttcgacg gcgcggttga tgccgacctg ccacgaacgc atagcgacat ataaaaaagg
     9601 cagggtaagc ggcagggcga tcagtaggat gaggccggta agccaaatgg gtattttttt
     9661 aggagacata gtgtttttca tcggcaaaac gggcggacag tataaatgtc cgcccgtttg
     9721 acaatccgaa aacggcttat ttcataccgg cttgctcaag cagccgggtg gcgtgttctt
     9781 tttcggaaac agtggtggcg gacacttggg gtgcttccaa cttggcgatg ggttccaaat
     9841 tgaaggtgga taccacgtgc ggattcaaag gatattcggc acggacggcg gtcagggcgc
     9901 gctgtccttc cttgccggcg aggaaggcga cgaatttttt cgcctcatcc ttgttttggg
     9961 aggattttaa cacggctgcg ccggaatagg taacgagtgc gccgggatct ctgtggcgga
    10021 cgaaattcag gcgggtgtgg acattttgta cgcctttttc acgcgcgaaa gcgtgccagt
    10081 agtagttgtt gatgagggcg gcatcgattt cgccgttttc aaccgcttga agggcgacgg
    10141 agtttttagc gtaaggcttg ccgtattctt tcaggccttt gagccatttc aatgcggccg
    10201 cttcgccttt cagtttgacg atggcgacaa tctgttccaa gaatgcgccg gaagtgggga
    10261 cgtaaccgat gcggtttttc catttcggcg tggcgtaatt caggacggat ttttccaaat
    10321 ctttttcaga cagtttgcgg gtgtcgtaaa cgacgacgcg cgaacgtccg ctcagtgcca
    10381 cccagtcttt tttggcggca accggcacgc ctttgccgcg tgtttcgttg atggtggagg
    10441 cgggcagggg ctctaggagg ttggctgcgg aaagggtggc gagtgccggg atttgttcgg
    10501 aatagaatac gtcggcgggg cttcggctgc cttcttcttt gatttggccg gcaagctggt
    10561 cgcctttggc actgttgagt ttgactttga tgccggtagc ccgggtaaag gcatctgcaa
    10621 cggcttgtgc cgcttctttg tgttggccgt tgtacacggt aatgtctgcc agcgcggggg
    10681 tggcggcggt cagggctgcg gcaagcagtg cgtatcggat agatgttttc atatcgattt
    10741 tctcctaaat gaatgagggt gtataccttg ttaagacata acggggtgta
//
LOCUS       DAWTHD010000081        10342 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWTHD010000081 DAWTHD010000000
VERSION     DAWTHD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.081"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(286..1197)
                     /gene="crgA"
                     /locus_tag="WM704_001604"
     CDS             complement(286..1197)
                     /gene="crgA"
                     /locus_tag="WM704_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFA9340989.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARNLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            1436..2014
                     /locus_tag="WM704_001605"
                     /pseudo
     CDS             1436..2014
                     /locus_tag="WM704_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(2222..3607)
                     /gene="pntB"
                     /locus_tag="WM704_001606"
     CDS             complement(2222..3607)
                     /gene="pntB"
                     /locus_tag="WM704_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFA9340990.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVLKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(3687..4070)
                     /locus_tag="WM704_001607"
     CDS             complement(3687..4070)
                     /locus_tag="WM704_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFA9340991.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(4074..5612)
                     /locus_tag="WM704_001608"
     CDS             complement(4074..5612)
                     /locus_tag="WM704_001608"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFA9340992.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            5901..6734
                     /gene="serB"
                     /locus_tag="WM704_001609"
     CDS             5901..6734
                     /gene="serB"
                     /locus_tag="WM704_001609"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFA9340993.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFGQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            6798..8021
                     /locus_tag="WM704_001610"
     CDS             6798..8021
                     /locus_tag="WM704_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFA9340994.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAAFFRRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            8182..9762
                     /gene="purH"
                     /locus_tag="WM704_001611"
     CDS             8182..9762
                     /gene="purH"
                     /locus_tag="WM704_001611"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFA9340995.1"
                     /translation="MSVIKRALISLCDKAGAVEFAQTLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIHPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLAAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TCIAARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGVRHFRH"
BASE COUNT         2373 a         2750 c         2797 g         2422 t
ORIGIN      
        1 ggtcttcggc gcgtctttta cgccaaaccc ataagaattt tgagggtaat cttcatcgtt
       61 ttgcctgaat tgccgacgca aagcgattta tcggcggttg tgtttgagcg tttgattgca
      121 gcttgcccaa tctcaaatat ttctaaatac caattgaaat tcaatatttt ataaaaacac
      181 tgatttggaa cgaacagatt tataaaaatt ccgtgtatcc ttgagtaagg gagttgattg
      241 aaaagacttg tttgattgat gatgaagaat aagttaatta atgtcttatt tggtattcgt
      301 tctattcata ttttttccca gttccttcac taaaaaatcc aaaaatacgc gtaagcggag
      361 gttgacggct ttatcgctgt aataaacagc attaaagggg tgtgttttat tggaggtttg
      421 ttcggcgaat aggggaatta actttccttc agtgatgtcg ttgtcaacca aaaaatctga
      481 taagcaagca ataccgcaac ttgaaaggca caacgagcgt aagatttcac cgctgctggc
      541 ggtaaagtgc ggtgaaattt tatagggatt tccctgcgca tctaaaaccg cccatgtatt
      601 tagagaaccg ggttctgtga agcctaaaca ttgatggttg gcaagatctt ctgcagattg
      661 tggcgtaccg tgttttgcta aatattcagg actggcgact acgcggaagt ggctgtcaaa
      721 cagattgcgt gcacgcagcc cggaatcgtc caattctccg gcccgtaagg caatatcgac
      781 tttgcgttca atcagattga tatagccttc ggaagaaacg agcgaaagtc ggatatgcgg
      841 atagcgttcg ttgaattttg ctgccagcgg cgccagcaga tgcagcacca tcggcatcgc
      901 ggaatccacg cgcaacacgc cttgcggtac ttcgtgcact gccagcattt cggtttccgc
      961 cgctgccatt tcttgcagga ttctctgcgc gcggcggaaa tattgcgcgc cttcttccgt
     1021 cagattgagt tgccgcgtgg tgcggttgag caggttcacg cccaactttt cctccagccg
     1081 tttgacgatg cggcttacgg cagaatttgc catctccaac tgctccgccg cacggctgaa
     1141 gctgccgctt tccaccactt gaacaaatac ggtcagttct tctgaattgg ttttcatcgt
     1201 gtttcctttt cggttggaac cccacccttt agggcggcag gatcagactt tatttgggaa
     1261 gggcgtaacc ccttcccaat caggacggca catagggaga cgatttatgt gtcatcccgt
     1321 gtgttgaaac atctgattat tccatttgac gcaaaagtgt tttcttattt ttgcactttt
     1381 aaatcataaa gtaaaacggt acaatacatt catcaattca caaacgaggt aacaaatgaa
     1441 tattttatta ttagacggcg gcaaagcgtt cggacattct cacggcgggt taaaccacac
     1501 gcttcacaaa aaagcgaaag aagttttgac cgcgctcgga cacaatgttt aagaaaccgt
     1561 gattgatgcc ggctatgatg ttgaggcgga aatcgaaaaa ttcgtttgga tggatgctgt
     1621 gatttggcag atgccgggct ggtggatgca cgagccttgg acagtgaaaa aatatatgga
     1681 cgaagtcttt accggcggac acggcaaact ctaccaaagc gacggcagac accgcgtcaa
     1741 tccgactgag ggctacggca caggcggctt gttgcaaggc aaaaaacata tgctttcact
     1801 gacttggaat gcgccgattg aagcctttac ccgcgaaggc gatttctttg aaggcaaagg
     1861 cgttgatgtt ttgtatatgc acttccacaa agccaacgag ttcatcggct tgagccgcct
     1921 gccgacattc ttatgtaacg atgtggttaa aaatccgcaa gtggaaaaat acttggcaga
     1981 ttatcaggca cacttggaaa aagtgttcgg ctaattaaaa atccatcttc aacacggaga
     2041 tggattttgt ttgtttcgtt gattttgtgt cagtttcaga tgtgggtgct tatccggacg
     2101 ggtcgtctga aaatgtttgc cccaatgcaa aaaaatcact gcaaaccttc acaaacgggg
     2161 ctggtagtga ttttttcaaa tcaaacagat tgaaaacctg cgccgaattg ttcagacggc
     2221 attatttttt caattccgcc aaaatcccgt caacggtttt cttcgcgtcg ccgaaacaca
     2281 tcacgctgtt ttcgttgaag aacagcgggt tttgtacgcc tgcgtagccg gtgttcatcg
     2341 agcgtttcaa tacgacgact tcttttgcct tccatacttc taaaaccggc atacctgcaa
     2401 tcgggctgtt cggatcggtt tgggcggcag ggttgacggt atcgttcgca ccaatcacca
     2461 agaccacatc ggtttcgggg aagtcgtcgt tgatttcgtc catttccaaa acaatgtcgt
     2521 aggggacttt ggcttcggcg agcagtacat tcatatgacc gggcaggcgg ccggcgacgg
     2581 ggtggatgcc gaagcgtact tcgatgccgt ttttacgcaa aagctcggtg atttcggcaa
     2641 cggggtattg tgcctgcgcc actgccatgc cgtagcccgg ggtgataatg acgctgcttg
     2701 cgcctttcag catttcggca acatcggcag ctttgacttc tcggtattcc tctatctctt
     2761 ggctgccgga agacgatatg ccgctgtcgc tgccgaaacc gccggcaatc acggaaacaa
     2821 acgaccgatt catggctttg cacataatgt aggacagaat tgcgccgctt gagccgacca
     2881 gcgcgccggt aacgatgagc aggtcgttgg agagcataaa gcctgccgct gcggccgccc
     2941 agccggagta ggagttgagc atagatacga ccacgggcat atccgcgccg ccgatggagg
     3001 caaccaagtg ccagccgaat gcgagggcga tcagggtcat aatcagaagg atgaagccgc
     3061 tgccgtcaat gccgacaaat acgagcagca acacaaacga tacggcgagc gccagtgcgt
     3121 tgagcttgtg tttggcgggc agttgcagcg ggctgctgct gattttgccg ttgagtttgc
     3181 cgaatgcggc cagtgagccg gtaaaggtta ccgcgccgat gaaaatgccc aaatagactt
     3241 cgaccagatg gatggtgtgc atatcgtgcg aaacgttgcc cggctcgatg tagctgttga
     3301 agccgaccaa aacggctgcc aagccgacga agctgtgcag aagtgcaatc agttcgggca
     3361 tttcggtcat ttctactttt ttggctttgt ggatgccgat tgccgcgccg atgagcatgg
     3421 cgatgataat ccagcccagt ccgtgggtat tgtcggaaaa gaccgttacg aagagggcga
     3481 ccgccatacc ggcgataccg gaatagcagc cccgtttggc ggtttcctgt ttggacagcc
     3541 ccgccagtga gaagatgaat aaaattgcgg caacgatata cgccgctgtt acgagtcctg
     3601 aagacatgga aattctccga ttttcgatga tttgttttca atgccgtctg aaaaattgac
     3661 gttcgtgttt tcagacggca tctgtttcaa gcagccgcga caaacagtcc gacggtgcag
     3721 gcaaatccgc ccgcccacat cagcgagcgc aatgctgctt tgtctgcgat gtagcaccag
     3781 ataaaggcga ggcggaacag gatgaacaat ccggcaagcg tgttgacggt tgcttgtccg
     3841 gcattgccgg ttgcgtgtgc cgtcaaaacg gcggcggcaa acggtgcaaa ggcttcaaaa
     3901 ccgttttgct gcgcggcgtg ggcacgggcg gctgcgcctt gcgtatgtgc cagaaaaccg
     3961 cgaggattgt ggttgtcttt aaaccggaat ccgcccgctt ttttggcata cgccgcacaa
     4021 aaaagcggca ataggcaggc aatcagaatg caccaatagg cgaaagtcat ggcttaccct
     4081 ttcttaaaca tattcagcat acgccgtgta accgcaaagc cgccgaagat attgatgccg
     4141 gcaatcagga tggcaacaaa cgacagcagc gaaacgaagc cgttgccctg accgatttgc
     4201 agcagcgcgc cgacgacgat gatgccggag atggcgttgg ttaccgacat cagcggtgtg
     4261 tgcagcgagt ggctgacgtt ccaaacgaca tggtagccga tgacgcaggc gaggacgaag
     4321 acgataaagt ggttcaagaa tgctgcgggt gcgaccgcgc cgacccacag caccaatacg
     4381 gcggcgatgg cggcgggcgc gagttttttc cacaggggaa caggtttcgg ctcgggcttg
     4441 gcggcaggcg cggctttttc agacggcgtt tgctgcggcc gggcggaaac ctgaatcggc
     4501 ggaggcggga aggtgatttc gccgtcgcgg gtaacggtca tattgcggat aatcacgtct
     4561 tcgaagtcca gcgtgatttc gccgtctttg ttcgggctta acagcttggt caggttcacc
     4621 aagttggtgg cgtaaagctg ggaagactgt ccggcaaggc ggtttgccat gtcggtgtag
     4681 ccgatgattt tcacgccgtt gccggttacg gacaattcgc ccggtcgggt gagttcgcag
     4741 ttgccgcccg tcgccgccaa atcgacgatg acggatccgg atttcatgct ttccaccatt
     4801 tctttggtaa tcagcttggg agcgggtttg cccggaatgg cggcggtggt gatgatgatg
     4861 tccacttctt tcgcctgttc ggcaaagagc ttcatttcgg cggcgataaa ttcgtcgctc
     4921 atcactttgg cgtagccgtc tccgctgccg cccgattctt gcaggaagtc gagtttcagg
     4981 aacttaccgc ccatcgattc gatttgttcc gccacttcca agcgggtatc gaacgcgcgc
     5041 accactgcgc cgagcgaatt tgccgtaccg attgccgcca aacccgccac acctgcacca
     5101 atcaccaaaa cctgcgcagg cggcactttg ccggcggcag tgatttgacc ggtgaagaaa
     5161 cggccgaagg cgttggcggc ttcaatcacg gcgcggtagc cgctgatgtt tgccattgaa
     5221 gacaaagcgt ccaaggcctg agcgcgggaa atgcggggaa ccatgtccat cgccagcgcg
     5281 ttgactttct tggcgcgcaa ggcctcgacc aaagcctcgt tttggcgcgg ccacaggaag
     5341 ctgacgatgg tttgaccttc tttgagcagc ggcagctcgc cttcggacgg cgcgttgacc
     5401 ttataaatta aagggcaggc ccaaaccgcc gctttgtcgg caacggttgc gcctgctgtt
     5461 tggtaagcgg catcgtccaa acttgccgcc aaacctgcac cgctttcgac aacggtttca
     5521 aagcctagtt tgcccagcag ggcaacggtg gcgggggtgc aggcgacgcg ggtttcgccg
     5581 gataatgact cgcgtgggat accgattttc atctctgaat cctttttcgg gttgttcata
     5641 tgtatcgtgg gttaaattta aatcggtgca gggcggagca acgccgtacc ggtttaaaac
     5701 cgattcactt caaatgttaa tatattttag ataatcccct tataacgaat tttcatcagg
     5761 ctggcaatag ttgcgcattt tcccgtgttg tccgacacat actgccgttt gcgtttgaag
     5821 tcgttttccg attgcaggca cgcccgccgt aacattcttt gccgtttcac tatataatcc
     5881 gcattttttg agccgccgtt atgccgcaag ccctcgtcct ccaatttccc tccgccgaag
     5941 ccctgccttc cgacttcccc tcacgcctgc ccgaacctga ttatgctgat gaaaagcgta
     6001 tgcgttttat tgttgaagaa ggattttctt taagcgaaaa agacgcggcg ttactggaca
     6061 gccgtcaaat cgaccacgcc gttttgccca atatggcgtt tggcgaactc gggctgattg
     6121 tcagcgatat ggattcgacg ctgattacca tcgaatgtat cgatgaaatt gcggcaggtg
     6181 tgggtttgaa aggcaaagta gcggaaatta ccgagcgcgc catgcgcgga gagttggatt
     6241 tcggacagtc tttacgcagc cgcgttgcgt tgttggcggg attggacgaa cagattttgg
     6301 cggacattta tgaaaacgtt ttgaagctct cgcccggtgc ggaatttttg ttggacgaat
     6361 gcaaaaggca caatgtgaaa ttcctgctgg tgtccggcgg gttcacgttt tttaccgaaa
     6421 ggctgcaaca acggctcggc ttcgaatacc aacacgccaa tattttggaa attgaaaacg
     6481 gcaggttgac cggccgtctg aaaggcagaa tcatcgacgc gcaggcaaag gcggatttat
     6541 tgcgcgaata ccgcagccgc ctcggattgc agccgcatca ggtgttggcg atgggcgacg
     6601 gtgcgaacga tattccgata ctcaaagaag cgggcatagg cgtggcttac cgcgccaaac
     6661 cgaaagcgcg ggccgccgcc gatgcctgta tcaacttcgg cggtttggag cgtgtacgcg
     6721 gcctgttcgg ataggcggat aggaaacgga tgccgtctga aaggttttca gacggcattt
     6781 gaacggcagg aacgacagtg ggacgcagaa aactttggtt tgccctggcg gcagcaggcg
     6841 ttatcggcgg tttggtcggc attgtgctga cggaactgat gcacttcata cggcatacgg
     6901 catacggtta tgacgcggac ggtgcgtaca tctcgttccg cgaaggtgtg gcacaggctt
     6961 tcggtatgcg gcgcgttgcc atgctgacgc tgtgcggcgc gatcgcaggc ggcggctggt
     7021 ggctgctcaa acgtttcggc aagccgcaaa tcgaaatcaa agccgccttg aagcagccgt
     7081 tgcaggggct gccgtttctg acgacggttt tccatgttct gctgcaaatc ataacggtcg
     7141 gactcggttc gccgctcgga cgcgaagtcg ccccgcgtga aatgactgcc gcgtttgctt
     7201 ttgccggcgg caggcggttg ggtttggacg aaggcgaaat gcgcctcttg attgcctgtg
     7261 cgtccggagc gggtttggcg gcggtgtata acgtgccgct cgcctccacg ctgtttatcc
     7321 tcgaagcgat gttgggcgtg tggacgcagc aggcggttgc cgccgcattg ctgacttccg
     7381 tcatcgccac cgccgtcgcg cgcatcggct taggcgacgt gcagcagtat catcccgcca
     7441 accttgccgt aaatacttca ttgctttggt tttccgccgt catcggcccg atacttggcg
     7501 caaccgccgc ctttttccgg cgcaccgccc aaaaattccc cttcatcaag cgcgacaata
     7561 tcaaaatcat tcccttggcc gtctgtatgt ttgcactcat cggtgtgatt gccgtttggt
     7621 ttcccgaaat tttgggcaac ggcaaagcag gcaaccaact gacttttggc ggtttgaccg
     7681 gctggcaata cagccttgag ttgaccgctg tcaaatgggc ggtcgttttg atggcgctgg
     7741 cggcaggcgc atacggcgga ctgattacgc cgtctatgat gctcggcagc accatttctt
     7801 ttgcagccgc cgccgcgtgg aacagcgtct tccccgaaat gccgtctgaa agcgcggcag
     7861 tcgtcggagc ggcggtgttt ctcggcgtat cgctcaaaat gccgctgacc gccattgtgt
     7921 tcgtattgga actgacctac gcgccgctgt ccctgctgct gcctttgtgc atcggcatgg
     7981 cgggggcatt gggaacatcg ggaaaaatgg gcttcaaatg aatgccgaca aatgccgtct
     8041 gaaaggtttc cagacggcat ttctttgtaa attgtcaaca atatctgcaa aaacctactg
     8101 ccaaaaaact gaaaatagcg gataataccg ccccgaaaaa gatccccttt caccatccaa
     8161 ccgtcagaaa aggaagcccc aatgtccgtc atcaaacgcg ccctgatcag cctgtgcgac
     8221 aaggcaggcg cggtcgaatt tgcccaaacc ctgcacaagc tcggtgtcga aatcctctcc
     8281 accggcggca cggcgaagct gcttgccggt gcgggcgttc ccgtgattga agtcgccgac
     8341 tataccggtt tccccgaaat gctcgacggc cgcgtgaaaa ccctgcatcc taaaattcat
     8401 ggcggcatcc tcggtcggcg cgatttggac gaacacgtcg ccaagatgga agaacacggc
     8461 atcggcaaca tcgacctcgt gtgcgtcaac ctctacccct tcgccgccac catcgccaag
     8521 cccggctgca cactggaaga cgcgattgaa aacatcgaca tcggcggccc gaccatggtg
     8581 cgctccgccg cgaaaaactg gaaacacgtc gccatcgtta ccgacaccgc cgacttcccc
     8641 gccattgccg ccgaactcga agccggcaac ggcgcgttga gcgataaaac ccgcttcaac
     8701 ctctcacgca aagcgttcag ccacaccgcc caatacgacg gcatgatttc caactacctg
     8761 acctcccttt cagacgacgt tttgagcggc cagccccaaa tcggcgaatt cccaagccgg
     8821 ttcaaccaaa gctggattaa agtgcaagat atgcgctacg gcgaaaaccc tcaccaacgc
     8881 gccgcgttct accgcgatat tcatcctgcg gcaggcagcc tttccgccta caatcaactg
     8941 caaggcaaag aattgtctta caacaacatc gccgatgccg atgccgcttg ggaagccgtc
     9001 aaatccttcg acgcgcccgc ctgcgtgatc gtgaaacacg ccaatccgtg cggcgtagcc
     9061 gttgcagccg ataccttggc cgcctacaaa ctcgcctacg ccaccgacac caccagcgcg
     9121 ttcggcggca tcatcgcctt caaccgcgaa gtcgacggcg aaaccgtcaa acagattacc
     9181 gacaaccaat ttatggaagt cctcatggcg ccgaagttta ccgccgaagc cctcgaaatc
     9241 gccgccgcca agaaaaacgt gcgcgtattg gaagtgccgc tcaaagcagg tgccaaccgc
     9301 ttcgaactca aacgcgtcgg cggcggactg ttggtacaaa ctcccgacat caaccgcatc
     9361 aaccgcgccg atttgaaagt cgtctccaaa cgccaaccga ccgagcagga atggaacgat
     9421 ttgctgtttg tctggaacgt cgccaaatac gtcaaatcca acgccatcgt gttcggcaaa
     9481 ggcggtcaaa cctacggcat cggtgcaggc caaatgagcc gcgtggacag tacctgtatc
     9541 gccgcccgca aagcgcaaga tgccaatttc gacctcaacg gcgcatgtgc cgcttccgat
     9601 gccttcttcc cgttccgcga cggcgtggac gtgattgccg aacagggcat caaagccatc
     9661 atccatccgg caggctcgat gcgcgatcag gaagttttcg acgcggcgga cgaacacggc
     9721 atcgccatgg cggtaacggg tgtgcgccac ttccgccatt aaggcagacg aacaaggcaa
     9781 tgccgtctga agggctttca gacggcattt tgcgccattt accgtaatcc tgaaaccccc
     9841 cattcccgcg caggcaggaa tctagatgag aatccatcct gtcagaaata ctcgaatcgt
     9901 caggaatacc cgtgtcatca ttcccgatac accgtaatcc tgaaacccgt cattcccgcg
     9961 cagacagaaa tccggatttg tccgcgcgga aacttatgcg ccgccattcc cgcgaaggcg
    10021 ggaatccgga ccgttcggtt tcggttattt ccgataaatt cctgctgctt ttcattccta
    10081 gattcccact ttcgcgggaa tgacgaagga gtgggaatcc ggtttttgag ttccggccat
    10141 ttccgacaaa ttgctttggc attggatatt tcctatttta atccactata gtggattaac
    10201 tttaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattccc taaggtgctg
    10261 aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
    10321 tgatttttgt taatccgcca ta
//
LOCUS       DAWTHD010000082        10304 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWTHD010000082 DAWTHD010000000
VERSION     DAWTHD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10304
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.082"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..478
                     /locus_tag="WM704_001612"
     CDS             <1..478
                     /locus_tag="WM704_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9340996.1"
                     /translation="LRMGLPLYIASLRRGAIYKVWQFVEDALRAVVPADSFEPTAQKL
                     KLFKVGAATILFYEDQNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGAG
                     ANLQHYNPLPDVAIAKAWNIPENWLLRAQMVIGGIEGAAGEKVFEPVAERLKVFGA"
     gene            complement(533..988)
                     /locus_tag="WM704_001613"
     CDS             complement(533..988)
                     /locus_tag="WM704_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFA9340997.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1244..2614)
                     /gene="ffh"
                     /locus_tag="WM704_001614"
     CDS             complement(1244..2614)
                     /gene="ffh"
                     /locus_tag="WM704_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFA9340998.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            2836..3642
                     /locus_tag="WM704_001615"
     CDS             2836..3642
                     /locus_tag="WM704_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFA9340999.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            4099..5639
                     /locus_tag="WM704_001616"
     rRNA            4099..5639
                     /locus_tag="WM704_001616"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            5740..5816
                     /locus_tag="WM704_001617"
     tRNA            5740..5816
                     /locus_tag="WM704_001617"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5774..5776,aa:Ile,seq:gat)
     gene            5822..5897
                     /locus_tag="WM704_001618"
     tRNA            5822..5897
                     /locus_tag="WM704_001618"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5855..5857,aa:Ala,seq:tgc)
     gene            6245..9136
                     /locus_tag="WM704_001619"
     rRNA            6245..9136
                     /locus_tag="WM704_001619"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            9233..9346
                     /gene="rrf"
                     /locus_tag="WM704_001620"
     rRNA            9233..9346
                     /gene="rrf"
                     /locus_tag="WM704_001620"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            9636..9998
                     /locus_tag="WM704_001621"
     CDS             9636..9998
                     /locus_tag="WM704_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFA9341000.1"
                     /translation="MLIRHIVARCGLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNI
                     NAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPAVLAKLKDQASV
                     GAPAPKGPAKPVLPAVKK"
BASE COUNT         2574 a         2357 c         2956 g         2417 t
ORIGIN      
        1 gttacgcatg ggattaccct tatatattgc gtccctaaga aggggcgcaa tatataaggt
       61 gtggcaattt gtcgaagacg cgctgcgtgc cgtcgtgcct gccgacagtt ttgaaccgac
      121 cgcgcaaaaa ttgaagctgt ttaaggtggg cgcggcaacc attttgtttt atgaagatca
      181 aaatgtcgtc aaaggtttgc aggagcagtt ccctgcttat gccgccaact ttcccgtttg
      241 ggcggaccag gcgaacgcta tggtacagta tgccgtctgg acgacacttg ccgcggtcgg
      301 tgcaggtgca aatctgcaac attacaaccc cttgcccgat gtggcgattg ctaaagcgtg
      361 gaatattccc gaaaactggc tgttgcgcgc gcaaatggtt atcggcggta ttgaaggggc
      421 ggcaggtgaa aaggtctttg aacccgttgc ggaacgtttg aaagtgttcg gcgcataatt
      481 tcgcggtcaa aaaaatgccg tctgaacccc gttcagacgg catttttcag tatcaggcgg
      541 cgagtttttc gcattctttg atacagtcga ggcaggactc gtaacaggct ttgcattccg
      601 catggtgggc ggagtgttct ttacaggctt tggcacactg tttgcacgct tcgaggcaca
      661 cttttgccgc gtcgcgtgtc agaggggaat tttgtgcggc aaggtcgtgc accgcgccgc
      721 ataaggcaag catctggcgc acggcaaccg cacagtcgga catagacgtg tcgccctgag
      781 tgagcaggga caggcagtgg gtcaggcaaa cctgtccggc atccagacag tgtgcggcag
      841 tttggcggac ggcggtgtag gcggatgcgg cggcaggctg catatcgtga tgatggtggt
      901 agtcggcgtg tccgtgcgca cgcgcgaaag aggcggcgga agccaaagag acggcagcgg
      961 cgctgcccaa aaattgacga cggttcatga ttttttcctt tatgtttcaa catgcgggtc
     1021 cggcataagg tacacactta ccatactgcc ctggccggat cggtacgatt ttctgaataa
     1081 ctgcttgatt ttgcaggtgt gaaattgtcg cacgagcagg catgttttag ctattcggaa
     1141 attcgaggaa gggtgcatca acctgttttg aaatgatgcg cagacatggt aacagacaaa
     1201 aagccgtctg aaaatcgttt tagacggctt ttgttttcaa acattaaaga ccgggaaaca
     1261 ttcccttcat tcccttcatg cctttggcca agcgcatcag tttgcccaaa ccgttgccgc
     1321 tgaacatctt catcatctgc tgcatttgtt caaactgttt gagcaatttg ttcacttcct
     1381 gcacggtcgt gcccgcgccc atcgcaatac ggcgtttgcg gccggctttg agcagggcag
     1441 ggttggcgcg ttctttaggg gtcatcgagt tgatgatggc ttctactttg cccatcgctt
     1501 tttcagccgt accttcgggg atttgtttcg atatttgacc cagttcgccc ggcattttcg
     1561 acatcaggtt ttccaaaccg cccatattgc gcatttgctg gatttgttct ttaaaatcat
     1621 tgaggtcaaa gcctttgcct ttttgcagct ttttcgccat tttggcggcg gcttcttcat
     1681 caataccttt ttgaacgtct tcaatcaggg tcaatacgtc gcccatgccg aggatacggc
     1741 tggcaagacg gtcggggtgg aaaggttcga ggccgttgat tttctcgccg acgccgataa
     1801 atttaatcgg tttgccggtt acatggcgta cggacaatgc cgcaccgccg cgcgagtcgc
     1861 cgtccatctt ggtcaatacg acgccgttca gcggcagggc ttcattaaat gcctgagcgg
     1921 tatttaccgc atcctgaccc agcatggcat cgataacgaa caaagtttca accgggttga
     1981 ccaccgcatg aagcgctttg atttcgttca tcatctcttc atcgattgcc aaacggccgg
     2041 cggtatcgac catcaataca tcgtaaaaat gttttttggc gtaatcaacg gcggcagttg
     2101 caatctcaac cggtttttgg ttggtatcgg atgggaaaaa atctacgccg acctgttcgg
     2161 ccaacaggcg cagctgttca atcgcggcag ggcggtaaac gtcggcggat accaccaaaa
     2221 cttttttctt ctgatcgttt ttcagcaggc gggcgagttt gccgacggtt gttgtcttac
     2281 cggcaccctg caaaccggcc atcaaaatga tggcaggcgg tgaaaccgat aaatccagtg
     2341 ttttgttttc cttgcccatc agttcgacta aggcttggtt gaccacgccg ataaaggctt
     2401 ggtcaggcgt caggctgccc gccacttcct gaccgagggc cttttctttg acgttgttga
     2461 cgaactcttt gacgacaggc agggcgacat ccgcctcaag cagggcgagt cggacttcgc
     2521 gcaaggcttc tttaatattg tcttcggtca gtttggcctg cccccggatg tttttgaaga
     2581 cattgctgaa gcggccggtt aaattgtcta acatacttgg tccttggtct gaagtaagaa
     2641 tagcttgccc catcaggggc attctttgtt aaaataaaat caaaataatt tgatgcggct
     2701 tgtgtgccgg acagcatatc ggcaaatccg tcaaggcttg cccgaaacgg ggattttaca
     2761 attccaacgt taaaagttcc aatatttcat aagcggccgc atacggcgca acagtataga
     2821 tagagaaagt ccaccatgcc gacagttttc atctttttga cggcggttta cgcaggattg
     2881 ggtgcatttg catggcactg ccaacagcag gggcgcggcc gggattaccc gtggaagacg
     2941 gaattgccgg ttttgggcgc ggcattgacc gtccacggcg cggcactgct gatgccggtc
     3001 attcaagaca aaatcatcat tatgggcttc gggtattccg gcagcctgat tgtttggatg
     3061 atgctgttta tttattttgc cggcagcttt ttttatccgc tgcgcggagt gcagttgctg
     3121 ctgtatcctt gcgccgcact gatgctgctg tcaggtttgg ttttcccggg aaaattctcg
     3181 ggatatgaaa ttaccgactt tccctttatg ctgcatatcg gcacatcact gcttgcatac
     3241 gggctgttcg gcatcgcaac attattgtcc gttttgagct tgctgctgca ccggagcctg
     3301 caccgcagaa acttctccaa gctcgcagga ttcctgccgt cgctgctcag tttggaaaaa
     3361 ctcatgttcc aggccatgtg ggcaggtttc atcctgctga cctattccgt cgtcagcggc
     3421 acattctttg ccgaagccgt attcggcaaa cccatgacat ttacccataa aaccgtattc
     3481 ggcatattgt catggctgat ttacggagga ctgctgctca aacacagcat gaccgcatgg
     3541 cgcggcaaaa aagccgccgt gtggaccatc atcggatttg tcagccttat gattgcctat
     3601 atgggcagca agttcgtatt ggaaatcatt ctgaaaagat aagaagagcc aacagatgcc
     3661 gtctgaagcc ccgagtttca ggcggcatat tcacaaaggc gcatcagccg gaggagaaga
     3721 ggaggggttg ttggaggcgg cgcagcgttt ggcggagata aaaaacctta tccgacagcg
     3781 acatgacgaa tttccccaaa aaaatcccgc tgaaagtatt gactgttttc ccctgcgggc
     3841 gtatagttcg gttcttcgcc gctgccgaag cggcggacga actgaaaagt atagcacgga
     3901 atgtcggaaa tatcgagaga tatcttagac aggcggaagg gatactttat aatttcgcaa
     3961 cgctctttaa caaaacagat taccgataag tgtgagtgcc ttgggcctcg cactgtttga
     4021 aagacagacg agatgatgtt ttgaacattg tcctgtcggt ttctttgaag cagaccagaa
     4081 gttaaaaagt tagagattga acataagagt ttgatcctgg ctcagattga acgctggcgg
     4141 catgctttac acatgcaagt cggacggcag cacagggaag cttgcttctc gggtggcgag
     4201 tggcgaacgg gtgagtaaca tatcggaacg taccgggtag cgggggataa ctgatcgaaa
     4261 gatcagctaa taccgcatac gtcttgagag ggaaagcagg ggaccttcgg gccttgcgct
     4321 atccgagcgg ccgatatctg attagctggt tggcggggta aaggcccacc aaggcgacga
     4381 tcagtagcgg gtctgagagg atgatccgcc acactgggac tgagacacgg cccagactcc
     4441 tacgggaggc agcagtgggg aattttggac aatgggcgca agcctgatcc agccatgccg
     4501 cgtgtctgaa gaaggccttc gggttgtaaa ggacttttgt cagggaagaa aaggccgttg
     4561 ccaatatcgg cggccgatga cggtacctga agaataagca ccggctaact acgtgccagc
     4621 agccgcggta atacgtaggg tgcgagcgtt aatcggaatt actgggcgta aagcgggcgc
     4681 agacggttac ttaagcagga tgtgaaatcc ccgggctcaa cccgggaact gcgttctgaa
     4741 ctgggtgact cgagtgtgtc agagggaggt ggaattccac gtgtagcagt gaaatgcgta
     4801 gagatgtgga ggaataccga tggcgaaggc agcctcctgg gataacactg acgttcatgt
     4861 ccgaaagcgt gggtagcaaa caggattaga taccctggta gtccacgccc taaacgatgt
     4921 caattagctg ttgggcaact tgattgcttg gtagcgtagc taacgcgtga aattgaccgc
     4981 ctggggagta cggtcgcaag attaaaactc aaaggaattg acggggaccc gcacaagcgg
     5041 tggatgatgt ggattaattc gatgcaacgc gaagaacctt acctggtttt gacatgtgcg
     5101 gaatcctccg gagacggagg agtgccttcg ggagccgtaa cacaggtgct gcatggctgt
     5161 cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttgtcatt
     5221 agttgccatc attcggttgg gcactctaat gagactgccg gtgacaagcc ggaggaaggt
     5281 ggggatgacg tcaagtcctc atggccctta tgaccagggc ttcacacgtc atacaatggt
     5341 cggtacagag ggtagccaag ccgcgaggcg gagccaatct cacaaaaccg atcgtagtcc
     5401 ggattgcact ctgcaactcg agtgcatgaa gtcggaatcg ctagtaatcg caggtcagca
     5461 tactgcggtg aatacgttcc cgggtcttgt acacaccgcc cgtcacacca tgggagtggg
     5521 ggataccaga agtaggtagg gtaaccgcaa ggagtccgct taccacggta tgcttcatga
     5581 ctggggtgaa gtcgtaacaa ggtagccgta ggggaacctg cggctggatc acctcctttc
     5641 tagagaaaga aggggcttta ggcattcaca cttatcggta aactgaaaag atgcggaaga
     5701 agcttgagtg aaggcaaggt tcgcttaaga agggaaaccg ggtttgtagc tcagctggtt
     5761 agagcacacg cttgataagc gtggggtcgg aggttcaagt cctcccagac ccaccaagaa
     5821 cgggggcata gctcagttgg tagagcacct gctttgcaag cagggggtca tcggttcgat
     5881 cccgtttgcc tccaccaaaa ctttacaaat gaaagcaagt ttgctgtttt tagcagctta
     5941 ttttgatttg cgaagtagaa taacgacgca tcgatcttta acaaattgga aagccgaaat
     6001 caacaaacaa agacaatgag tttgttttga ttttttattc tttgcaaagg ataaaaaatc
     6061 tctcgcaaga gaaaagaaaa caaacatagt atttgggtga tgattgtatc gacttaatcc
     6121 tgaaacacaa aaggcaggat taaggcacaa caaagcagta agctttatca aagtagggat
     6181 ttcaagtttg cttacttagt caacgggtag gtaaacgaag tcaaagaagt tcttgaaatg
     6241 atagagtcaa gtgaataagt gcatcaggcg gatgccttgg cgatgatagg cgacgaagga
     6301 cgtgtaagcc tgcgaaaagc gcgggggagc tggcaataaa gctatgattc cgcgatgtcc
     6361 gaatggggaa acccactgca ttctgtgcag tatcctaagt tgaatacata ggcttagaga
     6421 agcgaacccg gagaactgaa ccatctaagt acccggagga aaagaaatca accgagattc
     6481 cgcaagtagt ggcgagcgaa cgcggaggag cctgtacgta ataactgtcg agatagaaga
     6541 acaagctggg aagcttgacc atagcgggtg acagtcccgt attcgaaatc tcaacagcgg
     6601 tactaagcgt acgaaaagta gggcgggaca cgtgaaatcc tgtctgaata tggggggacc
     6661 atcctccaag gctaaatact catcatcgac cgatagtgaa ccagtaccgt gagggaaagg
     6721 cgaaaagaac cccgggaggg gagtgaaaca gaacctgaaa cctgatgcat acaaacagtg
     6781 ggagcgccct agtggtgtga ctgcgtacct tttgtataat gggtcaacga cttacattca
     6841 gtagcgagct taaccggata ggggaggcgt agggaaaccg agtcttaata gggcgatgag
     6901 ttgctgggtg tagacccgaa accgagtgat ctatccatgg ccaggttgaa ggtgccgtaa
     6961 caggtactgg aggaccgaac ccacgcatgt tgcaaaatgc ggggatgagc tgtgggtagg
     7021 ggtgaaaggc taaacaaact cggagatagc tggttctccc cgaaaactat ttaggtagtg
     7081 cctcgagcaa gacactgatg ggggtaaagc actgttatgg ctagggggtt attgcaactt
     7141 accaacccat ggcaaactca gaataccatc aagtggttcc tcgggagaca gacagcgggt
     7201 gctaacgtcc gttgtcaaga gggaaacaac ccagaccgcc ggctaaggtc ccaaatgata
     7261 gattaagtgg taaacgaagt gggaaggcac agacagccag gatgttggct tagaagcagc
     7321 catcatttaa agaaagcgta atagctcact ggtcgagtcg tcctgcgcgg aagatgtaac
     7381 ggggctcaaa tctataaccg aagctgcgga tgccggttta ccggcatggt aggggagcgt
     7441 tctgtaggct gatgaaggtg cattgtaaag tgtgctggag gtatcagaag tgcgaatgtt
     7501 gacatgagta gcgataaagc gggtgaaaag cccgctcgcc gaaagcccaa ggtttcctac
     7561 gcaacgttca tcggcgtagg gtaagtcggc ccctaaggcg aggcagaaat gcgtagtcga
     7621 tgggaaacag gttaatattc ctgtacttga ttcaaatgcg atgtggggac ggagaaggtt
     7681 aggttggcaa gctgttggaa tagcttgttt aagccggtag gtggaagact taggcaaatc
     7741 cgggttttct taacaccgag aagtgatgac gagtgtctac ggacacgaag caaccgatac
     7801 cacgcttcca ggaaaagcca ctaagcttca gtttgaatcg aaccgtaccg caaaccgaca
     7861 caggtgggca ggatgagaat tctaaggcgc ttgagagaac tcgggagaag gaactcggca
     7921 aattgatacc gtaacttcgg gagaaggtat gccctctaag gttaaggact tgctccgtaa
     7981 gccccggagg gtcgcagaga ataggtggct gcgactgttt attaaaaaca cagcactctg
     8041 ccaacacgaa agtggacgta tagggtgtga cgcctgcccg gtgccggaag gttaattgaa
     8101 gatgtgcaag catcggatcg aagccccggt aaacggcggc cgtaactata acggtcctaa
     8161 ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aatggcgtaa cgatggccac
     8221 actgtctcct cccgagactc agcgaagttg aagtggttgt gaagatgcaa tctacccgct
     8281 gctagacgga aagaccccgt gaacctttac tgtagctttg cattggactt tgaagtcact
     8341 tgtgtaggat aggtgggagg cttggaagca gagacgccag tctctgtgga gtcgtccttg
     8401 aaataccacc ctggtgtctt tgaggttcta acccagaccc gtcatccggg tcggggaccg
     8461 tgcatggtag gcagtttgac tggggcggtc tcctcccaaa gcgtaacgga ggagttcgaa
     8521 ggttacctag gtccggtcgg aaatcggact gatagtgcaa tggcaaaagg tagcttaact
     8581 gcgagaccga caagtcgggc aggtgcgaaa gcaggacata gtgatccggt ggttctgtat
     8641 ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg attccgccca
     8701 agagttcata tcgacggcgg agtttggcac ctcgatgtcg gctcatcaca tcctggggct
     8761 gtagtcggtc ccaagggtat ggctgttcgc catttaaagt ggtacgtgag ctgggtttaa
     8821 aacgtcgtga gacagtttgg tccctatctg cagtgggcgt tggaagtttg acgggggctg
     8881 ctcctagtac gagaggaccg gagtggacga acctctggtg taccggttgt aacgccagtt
     8941 gcatagccgg gtagctaagt tcggaagaga taagcgctga aagcatctaa gcgcgaaact
     9001 cgcctgaaga tgagacttcc cttgcggttt aaccgcacta aagggtcgtt cgagaccagg
     9061 acgttgatag gtggggtgtg gaagcgcggt aacgcgtgaa gctaacccat actaattgcc
     9121 cgtgaggctt gactctatca tttgaaggac ttcaagagat aaaagcttac tgactgattc
     9181 agtcattacc gaatatattg attaaggctt taccgatttg caacagttta agtttggcgg
     9241 ccatagcgag ttggtcccac gccttcccat cccgaacagg accgtgaaac gactcagcgc
     9301 cgatgatagt gtggttcttc catgcgaaag taggtcactg ccaaacaccc tttcagaaaa
     9361 cccccggata tccgggggtt tttgctttgc ccggaaaaaa tgtcggggat ggcgggacgg
     9421 catctgtacg gtgtccggtc gggtttgcgg aggaacggct tgaaactttg ggatattcat
     9481 tttagaatga cccgttttat agcggattaa caaaaatcag gacaaggcgg cgggccgcag
     9541 gcagtacgga tagtacggaa ccggttcgcc cggtgcttca tcaccttagg gaaccgttcc
     9601 ctttgagccg gggcggggca acgccgtacc ggtttttgtt aatccgccat atcgtcgcaa
     9661 gatgcggttt gttgtttgca acccttaaag gaaaaaccat gaaaaaaatg tttgtattgt
     9721 tctgtatgct gttctcctgc gccttctccc ttgcggcggt aaacatcaat gcggcttcgc
     9781 agcaggagct ggaggcgctg ccgggcatag gcccggcgaa ggcgaaggcc attgcggaat
     9841 accgcgcgca aaacggcgcg ttcaagtctg tggacgattt gatcaaggtg aagggcatcg
     9901 gtccggcggt gctggcgaag ctgaaagacc aggcttccgt cggcgcgccc gcaccaaaag
     9961 gccccgccaa accggtgctg cctgcggtta aaaaataggg gaacctgtaa aggaaggggc
    10021 atcggctgcc gccggcgctt ttttgttctt tgtttggaag ggaaatggct ggagaaagac
    10081 ctgcgctata tcggggaatg aatcgatgca aaggaaatgc cgtctgaaca tgcgttcggg
    10141 cagcgttttt ataacaaaaa cacttaatgg cggttggttt tatacctatc taagtttttg
    10201 tgtcgtgcat atctgaaaag tgtattccgt ttcagtttgt aagctgtggc attctgagac
    10261 ctttgcaaaa aagcccttcc ttcgacagcc gaaacccaaa caca
//
LOCUS       DAWTHD010000083        10226 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWTHD010000083 DAWTHD010000000
VERSION     DAWTHD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.083"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(159..635)
                     /gene="greA"
                     /locus_tag="WM704_001622"
     CDS             complement(159..635)
                     /gene="greA"
                     /locus_tag="WM704_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFA9341001.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1026..2327
                     /gene="aroA"
                     /locus_tag="WM704_001623"
     CDS             1026..2327
                     /gene="aroA"
                     /locus_tag="WM704_001623"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFA9341002.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGGYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2405..2938)
                     /locus_tag="WM704_001624"
     CDS             complement(2405..2938)
                     /locus_tag="WM704_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9341003.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(2995..4521)
                     /locus_tag="WM704_001625"
     CDS             complement(2995..4521)
                     /locus_tag="WM704_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFA9341004.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(4633..6174)
                     /locus_tag="WM704_001626"
     CDS             complement(4633..6174)
                     /locus_tag="WM704_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFA9341005.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELALSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            6322..7101
                     /locus_tag="WM704_001627"
     CDS             6322..7101
                     /locus_tag="WM704_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFA9341006.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREINV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            7098..7799
                     /locus_tag="WM704_001628"
     CDS             7098..7799
                     /locus_tag="WM704_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFA9341007.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            7796..9250
                     /locus_tag="WM704_001629"
     CDS             7796..9250
                     /locus_tag="WM704_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HFA9341008.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9399..9818)
                     /gene="fitB"
                     /locus_tag="WM704_001630"
     CDS             complement(9399..9818)
                     /gene="fitB"
                     /locus_tag="WM704_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFA9341009.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(9818..10054)
                     /gene="fitA"
                     /locus_tag="WM704_001631"
     CDS             complement(9818..10054)
                     /gene="fitA"
                     /locus_tag="WM704_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFA9341010.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         2435 a         2666 c         2819 g         2306 t
ORIGIN      
        1 gaaaatgttg ctttcccgca acatgattta aatacagacc ttgtcaaagc cgatgcggcg
       61 cagacccgga tgccgtctcg ttttttaaaa caccataaaa aaacacacgc ggtactttaa
      121 tttcctgcgt gtgtcgagtg tatcgaaatc aagccgaatc aaatataccg gacttcgata
      181 atatcgtatt cgcgtacgcc gcccggtgcc tgaacttccg ccgtatcccc ttcttccttg
      241 ccgatcaggg cgcgggcaat cggagagccg acatagattt tgccctgttt gatgtcggct
      301 tcgtcttcgc cgacaatttg ataggtaacg tgttcttccg tttccaaatc ttccagcgta
      361 accgtcgtac cgaacacgat tttgccttcg gcgtggattt cggccggatt gatgatgtgg
      421 gcaacggaaa gtttgtgttc caactcggaa atgcggccct cgataaaacc ttggcgttct
      481 ttggcggctt cgtattcggc gttttcggac aaatcgccgt gcgatctggc ttcggcaatc
      541 gcttcgatca cttcgggacg cgccacgctt ttgagctgct gcaattcctg tttcagcaat
      601 tccgcaccgc gtacggtcag ggggattttt tgcatcggtc tttttctcca tattccggca
      661 caccggtttg cggcggcaag cataccgcgt accgtcttgt tttgtgcgtc cggatattaa
      721 aataaaaata caagccgccc ggaaaatcgg cggcttgtct gtcgttgaac agcggctatt
      781 ctaccaaatt ctatgaaatt ggcaatcgtg ccgtgccgcc ggcagacgcg ctatgtccgc
      841 aacaaaagcc gaaaatatgc cggcaaaaaa attttagaaa caaaaaattt aaaaaaaatc
      901 aattttcggc ataaaaaacc aaattcacgt actttaaaac cgaaaatgcc aaacctgaga
      961 ttttccagac ggcatttgca ccagtataat gcaggctgtt tttatcttta tatttacgtt
     1021 ttgccatgac cgaatccgtc cgcctccccg ccgcctcgct caaaccttcc accgtcgccc
     1081 tgcccggctc caaaagcatc agcaaccgca ccctgctgct cgccgccctg tccgacaatg
     1141 tttgcgaaat ccattccctg ctcaaatccg acgataccga ccgtatgctc gaagcactcg
     1201 ataaactcgg cgttcaaatc gaacatcttg ccgaaggccg tctgaaagta cacggcacgg
     1261 gcggacgctt ccccaaccgc tctgccgatt tgtttttggg caacgcgggc acggcgttcc
     1321 gtccgctgac cgccgcgctg gccgttttgg gcggcggtta tcatctgcac ggcgtgcctc
     1381 gtatgcacga acgccccatc ggcgatttgg tcgatgcgtt gcggattgcc ggcgccgatg
     1441 tcgaatatct cggcaacgaa cactatccgc cgcttcatat cggcaaacgt caagactgcg
     1501 gcgagcgcgt gattcctatt aaaggcaatg tgtccagcca gtttctgacc gccctcttga
     1561 tggcgctgcc gctgaccggg caggcgtttg aaatccgtat ggtcggcgag ttgatttcca
     1621 agccctatat cgacattact ttgaaactga tggcgcaatt cggcgtacag gttgccaatg
     1681 aaggctaccg cgttttcaaa atccctgccg atgcgcacta tcacgcaccc gaacacttgc
     1741 acgtcgaagg cgatgcctcc ggcgcgtcct acttcctcgc agccggtttg attgccgcca
     1801 cgcccgtccg cgttaccggc atcggcgcaa acagcataca gggcgatgtc gcctttgccc
     1861 gcgaactgga aaaaatcggt gcggacgtgg tttggggcga aaacttcgtc gaagtctcgc
     1921 gcccgaaagg acgcgccgtc caagcctttg atttggatgc gaaccatatc cccgatgccg
     1981 cgatgaccct cgccatcgtc gcgcttgcca caaggcaaac ctgcacgctt cgcaacatcg
     2041 gttcgtggcg cgttaaggaa accgaccgca tcgccgcaat ggcgaacgag ttgcgcaaac
     2101 tcggtgcaaa agtcgtcgaa gaagccgaag cgattcacat taccccgccc gaaacgccga
     2161 cacccgatgc cgtcatcgac acttacgacg accaccgcat ggcgatgtgt ttctcgctga
     2221 tttcgctgtt gggagtaccc gtcgtcatca acgacccgaa atgcacccat aaaaccttcc
     2281 cgacttattt tgaagtgttc tcatcactga ccgaaacagc ggaataaggc ggcaaaatgc
     2341 cgtctgaaac ccgaaaacag ggcttcagac ggcattttgc cgcgattccg gcgcggcggg
     2401 cggctcattc tgtaaaaaac gtatgcgctc caaggtagtt tttggcgtaa aacggtgtgg
     2461 agagtttttc ggttttgatg gttttgccgc tgccgggggc atggatgaat tcgccgttgc
     2521 cgatgtagag tccgacgtgt gagtagcggt gtgcgccgcc ggtgttgaag aatacgaggt
     2581 cgccggcctt gaggcggctg tcggggattt tgcggcttgc cgccgccatg tcgcgggcgg
     2641 tgcgcggcag cttgacgttg agggcgtttt tgtaaacgaa ttgaatcatg ccgctgcagt
     2701 cgaagccggt tgcggtgctg ctgccgcccc atttgtaggg cgtgccgatg agtccgaggc
     2761 tgtggagcat gagttcctgc gagccttgtg tgcggccgat gtggctgatg cggacggctt
     2821 ggatttgccg gactgtctgt ttgggtttcg gttggcggtg tttgccggag gtcgtgccgc
     2881 atgaggcgag gagcagtgcg ccgagacaga ggaaaagggt tttgtcgggg gaaaacatgg
     2941 ttttttcctt tgcgggttcg gatatgccgt ctgaaggtgt ttcagacggc atttctataa
     3001 taaaccttcg atgggcagca gggataggat ttttgcggcg atgcgtttcc aaagtttggc
     3061 ttcgggttcg ttcgggtagg tttttcgggt ggcgggatcg tgccattgca ggcggttgtg
     3121 tttgtcgagg gtaacgcggt aggcgtattc gggtgtggta tcggcgaggg tgcgctccat
     3181 ctgttctgcg attttggggc tttcgatgac gacgcccatt tcggtattga gccgtgcgga
     3241 acgggggtcg aggttgaatg agccgatgaa gatgcgtttg ccgtccacaa tgaaggtttt
     3301 ggcatgcagg ctggttacgg agctgccggt caggcctttg tcttttgtgg cggggacggc
     3361 atggttgggt tgcagctcgt agagtttgat gccggctttg agcagcggtt ttcggtattt
     3421 gacgtagccg gaatggacgg cggcaacgtc ggtcgcctgt agcgagttgg tcaggacggt
     3481 aacgtctatg ccgtcctgca ccagttttgc cagtgcgtct gtgccggatt ttgtagggac
     3541 gaaatagggt gaaaccagat agacgctttt ttcgggctgt ttgagcgcgt cttgcagcct
     3601 cccggcaatc ggcggtttgc ggcggtcgcg gtcgagtcct tttgcagggt tgtcgctgat
     3661 caggcgggtt tggacgctct gccagtcgat gcgtcccgtc tgtatttttt ggtagagggg
     3721 cgactgttcg acggtttcgc ggtagcgcag gagcgcgtgt ctggatgttt cgtcgttgta
     3781 tccgagtgct tgaagaccct tgccgatgtt gccgctgcgg atgatgcgcg tggcgttgtg
     3841 ggcggaatgg cttgcccagt agcggtcgaa gtcgtgcgat acttcgccga cgacgctgcc
     3901 ggtggcgagg atgtccaggt cggcgaaaac ggtgtcctca ccgactttga agtattcgtc
     3961 gccgatattg cgtccgccga gtatggtggc gcggttgtcg gcggtaaagg atttgttgtg
     4021 catgcggcgg ttgaggcggg ggaagtcggt caggtagccg agtgcgcgcc atttgcgtag
     4081 gacgaagggg ttgaacaggc gcacttcgat attgggatgg ctgtcgaggg cgagcaggag
     4141 atcgtccaac ccgcgcgtgt tgttgtcgtc caacagcagg cgtacgcgca cgccgcgttc
     4201 tgcggcaagg tacatgaggt tgaacagcag cctgccggaa atgtcgttgc gccaaatgta
     4261 gtattgcaaa tcgaggctgt gttcggcaga ttcgataagg gcggcgcggg cggcaaaggc
     4321 ttcgtggggg tcgtcgagca ggtagatgtc ggatagcccg ttgttatgag gggtgtgccg
     4381 gatttgcagg atgttgtcca ggaggacagg tttggaagta ttgaaatgac ggctttccgt
     4441 ccgttcttcc agtgggggca accatgaaga acatgaacag agaaggaggc ataaaaggga
     4501 aatgaggctg cgtgttttca tcggggatat agtttcagac ggcattgcct gtattttggg
     4561 gtctgtatgc attgaaatga agcatcataa tctaaaggtt ggaatgggta aaagttttgt
     4621 cgggatgcgg ggttaaggcc gcgtgcctct tgtcaggttt gcaccgtctg aaacgtgcag
     4681 acgcttaaaa accaacgacg aggcaagggt gatgccgccg atactgagca gggtcatacg
     4741 gaaggcgaaa tgcaggcctg aagaagccgg tatcagaaat gtccagtttt taaggattaa
     4801 tgcgccggca acaatgccca cgctgatggc aagctgttgg ttgaccgcca tcaggctgtt
     4861 gccgctgccg gtctgttgcg ggcgcaaatc ggcgagggtc agtgtgttca tggcagaaaa
     4921 ctgtagggag ttgcatgcgc cgatcgccag cgagaggaaa atccaaatcc acagcggcga
     4981 gtttccgtca ggcagggcga gcagcatgat gaaggcggca agcagcttgg tgttccaaag
     5041 cagtaccgtg cggtagccga aacgtttcat gagcggtgca atcagcggtt tgaccagcag
     5101 cgaagacagg gcgacgggtg cgaccagcca gcccgacagg cttgcgccga agccgaaagc
     5161 gacttgaaac atcaggggca tcagaaaagg aatcgagctg atgccgagac ggctgaacag
     5221 attgcccgcc agtcccaaac ggaaggtgcg tatcagaaac aggtcggcgg aataaatcgg
     5281 tttggacgcg gttttcatat gtcggaaata acggcgtgca aacagcagtc cgccgcacag
     5341 cggtaacagt gcaaaatacg gaggcagcgc gtgcgacagg ctttctgccg caagcagcag
     5401 caggcaggcg gcaacggaaa aggtcagata gcctttgaaa tccagagcgg tatcgttgcc
     5461 tttgacatcg ggcatgatgt tgcgtcccaa tatgaaaccc agcagaccga tgggcaggtt
     5521 gagcaggaaa atccagtgcc acgaagcgta ttcgaccaga taaccgcccg ccagaggtcc
     5581 caacgcaggg ccgatcagtg caggcatgac cgcataattg atggcgttga gcagcttcga
     5641 tttttcatat acacgcaaga tggtcagacg cggtatcgga accagcatcg aaccgccgat
     5701 cccctgaacg acacgggaaa gcgccaattc aaacagcgaa cccgatgcgg cgcacaatgc
     5761 cgatccgagc ataaaaacgg caatcgaacc gaaaaagact tttttcgttc cgaacttgtc
     5821 cgccaaataa ctgctcaaag gaatcagcag ggcaaccgtc agcgtatagg caacgactgc
     5881 ctgttgcata tccggaggcg attcgttcag gtcggcggca atttcgggca gtgcggtatt
     5941 taaaatggtc gcatccaaca tctgcataaa aatggcaatt gccagcagaa gcggcagcca
     6001 aggggatggt gcgcgggcgg acagggtgtt tttttccata gggtgattgt accccatcct
     6061 tgtgccgtta ttgttttcag atgctgtctg aatgccggca gagtcggcat cctgaatgtt
     6121 cacaagcaaa cgaatcggca ttgcattgta atgataatta ttatcgaaaa tcatcagagt
     6181 aaggtacagt aagcgttatg gggcagtttg taagaaaaac cggattattt tttaaaatta
     6241 gacttgaccc gcaacggtca attacttaaa gtaaacgctt acctttctac agaaaaaacg
     6301 ggtttcccgt tatcaaaaaa tatgagcgca accattcccc caaaaatcat ccgatacgac
     6361 agcaatccga cagatgtcta ttttttcggc acttgcgtcc ttgatctttt tatgcccgaa
     6421 gcaggcatgg atgccattgc cctaatcgag cagcagggca tacgcgtcca tttcccgatg
     6481 gcgcaaagct gctgcggtca gcctgcctat tcatccggcc atccgaccga agccttcgat
     6541 gtcgccaaag cgcaactcga ccttttcccc gaaaactggc cgattgtcgt accgtccggc
     6601 tcatgcggcg gcatgatgaa acatcactgg cctaccctgt ttaaaaatac agaatacgaa
     6661 tccaaagcgg ttgattgtgc caaccgtatc attgagttta ctcatttctt gcttgccatc
     6721 ggctacaagc ctgaagacaa aggcgagcca gtcaaagtcg ccgttcacac ttcctgtgcc
     6781 gcccgccgag aaataaatgt ccatctttca ggctggcagt tgattgacgg tatggaaaac
     6841 gtcgaacgca tcgtccacga ccacgaaagc gagtgttgcg gcttcggcgg cacattctcc
     6901 gtcaaacaag ccgatatttc cggcgcgatg gtaaccgaca aagttgccgc gctgaaagaa
     6961 accggcgcaa ccgaaatcat cagcgcggac tgcggctgta tgatgaacat cggaggcaaa
     7021 atcgccaagg acgagccgga tatgccgcgt ccgaagcata tcgcatcctt cttgttggaa
     7081 cgcaccggag gcaaagtatg agcgcgcgcg aaaatatttt ggcaaaactg aaaaaagccg
     7141 gcgcattgcc gatggaagag cctgcggttt ttgattatta ccgcgaaaag ggcgtttctt
     7201 gggacagcga agccgagcgt ctgaaacatt gggctgccgc catgcgcgcg gtcaaaaccg
     7261 aaatctattg ggtgacgaaa agcaattgga tgcaggtttt ccgcgaagcg gcagaaggca
     7321 agggtttaaa aaacatcctg ctgcccttgg cgaccgaaca cggacaaatt gcccgtgccg
     7381 cattggcggg cagcaatatc gacccgattg ccttcgagcg cgaaatcgat acttggaaaa
     7441 ccgagttttt cacgaacatc gatgcaggct tcagcggcgc gcaatgcggc atcgcccgca
     7501 ccggcacgct gatgctgttt tccagccctg aagaaccgcg tactttgagc ctcgttccgc
     7561 ccgtgcattt ctgcctgttc gatacgtcca agatgtacaa cgagtttcac aacgccgtcg
     7621 aaggcgaaaa actggtggaa aacggtatgc cgaccaatgt attcctgatt tccggcccgt
     7681 ccaaaaccgc agacatccag ttgacgctcg cttacggtgc gcacggtccg cgtgatttgg
     7741 tcatcctcgc cattttgccc gaccacattt cccctgccga tttggaggaa aacgcatgac
     7801 tacgcaaacc atcaagttcc acatgaagcc ggaaactttc aaacaaaacg ccgcaatttc
     7861 ccttcaagac aagcccttgc gtaaaagcct gcgtaccgcg atggatatgc tgatgaccaa
     7921 gcgcaaagcc gttttgaccg acgaagaaga gttgcaaagc ctgcgcgatt tgtgcgaaca
     7981 catccgtcag cgttccctct ccaaattacc cgcgctgctg gagcagttgg aagaaaacct
     8041 gactaagttg ggcgtgaaag tacactgggc ggaaacccct gccgaagcct gccaaattat
     8101 ccacgacatc atcacagcca aaaacggcaa gctgatggtc aaaggcaaat ccatggtcag
     8161 cgaagaaatc gagctgaacc attatcttga agcaaaaggc attaaggcgg tggaaagcga
     8221 cttgggcgag tttatcgtcc aaatggcagg cgaaaaaccg acccatatcg tgatgcccgc
     8281 aatccataaa accaaagaac aggtcagcga actgttccac caaaacctcg gcacaccgct
     8341 tacagacgac gtggaccaac tgaccggatt cgcccgcaaa gccctgcgcg atatttacag
     8401 cactgccgat gtcggcttga gcggcgtaaa cttcgccgtt gccgaaacgg gcacgctgtg
     8461 tctggtcgaa aacgaaggca acggccgctt gagtactacc gttccgcccg tacatatcgc
     8521 cattacgggt attgaaaaag ttgtcgccaa gctgtctgat gttccgcctt tgtacagtct
     8581 gctgccgcgt tccgccatcg gtcagaacat taccacttat ttcaatatga ttaccggccc
     8641 gcgccgcagt gaagaattag acggtccgca ggaaatgcac ttggttttgc tcgacaacgg
     8701 ccgcagccaa gcttatgccg aagaccaaat gcgccgcacc ctgcaatgta tccgttgcgg
     8761 cgcgtgtatg aaccattgcc cggtttatac acgcatcggc ggcgcggcat acggcacaac
     8821 ctatcccggt ccgattggcg agattatttc cccgcacctg ttgggtcttg atgccacccg
     8881 cgacctgccg accgcctgca cgatgtgcgg cgcgtgcgtg gaagtttgtc cggtacgcat
     8941 cccgattacc gaacaaatgc aacgtttgcg cgttgaagca caacgttcgc cgaccgaaac
     9001 cgtgccgcac cccatccgag ggcaaggcgc atcgcatacc ttcggcgaac aaatggcgtg
     9061 gcgcacattc aacggtattt tcagcggtag caaaacctac cgcgccttcg gttgggcagc
     9121 caccaagttc cgcaatctga ccccgcgcaa acagttgggt tggacgcaaa accgcgtacc
     9181 gatgaaaccg gcgaagaaaa ccctgcacga actgatggcg gaaaaaatgc gccaaaaaga
     9241 acaggcataa aaagttgttc gcaaaaatac cgtctgaaac ccgaaaacag ggcttcagac
     9301 ggcctgaaaa gcagcctgaa cgcgcaaaaa taaataatga aacggaatgc taagacgctt
     9361 tcaggcagcc tgacattaag gctgcttaaa agcgtctttt aatcgtgcca cggattgaac
     9421 accgcgacat cggccgcaaa aaatgagccg gtatcacgcg tagcaactgt caaactgtgc
     9481 tgttttgcag tggcggcaat atagccgtct gcggcagcaa tctctttgcc atgtgttttg
     9541 gcataggaac gaatttgcgc atagattgcg gcaaccggtt catcaaaagg cagaatccgc
     9601 cccgcaaata aaggcaaaat ggattgttcc aaacgttcgt gcagcacatt ctttttcttg
     9661 ccattgagca acaacgccac acccaaacgc aattctgcaa cagtaatggc agacaaatac
     9721 acatcttcca atatcaaact atccaaccat gccaccacac gttcattggg ttgtgggcgc
     9781 aaaggttcgg aaatcacatt cgtgtccagc aaaatcatca caaagaaacc tcgttatcag
     9841 tattacgacc gcgtacgtct tccagctcaa cacctccgat ttcctgccct attgatgcca
     9901 acattgaccc caaacgtaca gtttgttgtg ctttggcgat gttatccaaa attaagcgga
     9961 tttctgcttc ggtactgcgc cctgcggctc gcgcacggaa tttgattgcg ttgtgcgtgg
    10021 cctcggataa atttctaatc acaacagaag ccattgcctt tccttcctga aaaaagtttt
    10081 tcaaatgcta tcaaaataaa aaaaatgata gcaatctatt ttttaacgcc tgcggatgcc
    10141 tgaaaattca ggtggccgca ggcgtttttt tacatctaaa aaaacgtctc ccctccctct
    10201 ttctgataaa taaaaaacag tcgctc
//
LOCUS       DAWTHD010000084        10055 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWTHD010000084 DAWTHD010000000
VERSION     DAWTHD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.084"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            37..1503
                     /locus_tag="WM704_001632"
     CDS             37..1503
                     /locus_tag="WM704_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFA9341011.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(1707..2783)
                     /locus_tag="WM704_001633"
     CDS             complement(1707..2783)
                     /locus_tag="WM704_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFA9341012.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            2847..2984
                     /locus_tag="WM704_001634"
     CDS             2847..2984
                     /locus_tag="WM704_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFA9341013.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGWFR
                     L"
     gene            3006..4148
                     /gene="bioF"
                     /locus_tag="WM704_001635"
     CDS             3006..4148
                     /gene="bioF"
                     /locus_tag="WM704_001635"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFA9341014.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            4158..4805
                     /locus_tag="WM704_001636"
     CDS             4158..4805
                     /locus_tag="WM704_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HFA9341015.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            4793..5575
                     /gene="bioC"
                     /locus_tag="WM704_001637"
     CDS             4793..5575
                     /gene="bioC"
                     /locus_tag="WM704_001637"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFA9341016.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            5817..7254
                     /locus_tag="WM704_001638"
                     /pseudo
     CDS             5817..7254
                     /locus_tag="WM704_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
     gene            7257..8355
                     /locus_tag="WM704_001639"
                     /pseudo
     CDS             7257..8355
                     /locus_tag="WM704_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8367..8902
                     /locus_tag="WM704_001640"
                     /pseudo
     CDS             8367..8902
                     /locus_tag="WM704_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            8904..9546
                     /locus_tag="WM704_001641"
                     /pseudo
     CDS             8904..9546
                     /locus_tag="WM704_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            9567..9926
                     /locus_tag="WM704_001642"
     CDS             9567..9926
                     /locus_tag="WM704_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFA9341017.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
BASE COUNT         2572 a         2149 c         2600 g         2734 t
ORIGIN      
        1 cgatttttcg ggacggtctt ttttacggag gtcaatatga tgaaattggg tttcaaaccg
       61 atacccctcg ccattgccgc agtattgtgc gccctggttt tggcactgcc cgtacccgac
      121 ggggtcaagc ctcaggcttg gacgctgctg gctatgtttg tcggtgtgat tgccgccatt
      181 atcggcaagg ttatgccgtt gggcgcgctg tcgattattg ccgtcgggtt ggtcgcagta
      241 accggcgtaa ccgccgacaa accgggcgcg gcgatgagcg atgcgttgag tgcgttcgcc
      301 aatccgttga tttggctgat tgccatcgca gttatgattt cgcgcggttt gctcaaaaca
      361 gggctgggga tgcgtatcgg atatttgttt atcgccgttt ttggaagaaa aacgctgggc
      421 atcggttaca gtctcgctct ttccgaactg ctgctggctc ccgttacccc ttccaatacc
      481 gcgcgcggcg gcggcattat acatccgatt atgcagtcga ttgccggcag ttacggctcc
      541 aatcccgcaa aaggcacaga aggcaagatg ggtaaatatt tggctttggt caattatcat
      601 tccaatccca tttcgtcggc tatgtttatt actgcaactg cccccaaccc tttaatcgtc
      661 aacttgattg ccgaaaattt aggcagtagt ttccgtcttt cttggggggc gtgggcgtgg
      721 gcaatggctg ttcccggcgt tatcgccttt ttcgttatgc ctttgatttt atattttttg
      781 tatccgcctg aaattaaaga aacgcccaat gctgttcaat ttgccaaaga ccgtctgagg
      841 gagatgggta aaatgtcggc agacgaaatc attatggcgg tcattttcgg tatcttgctg
      901 ctgttgtggg cagatgttcc cgcccttatt accggcaatc acgcttttag tatcaacgcc
      961 accgccaccg catttatcgg attaagcctg cttttgcttt ccggtgtatt gacttgggac
     1021 gatgttttga aagaaaaaag cgcgtgggat acgattattt ggtttggcgc attgattatg
     1081 atggccgcat ttttaaataa actcggactg attaaatggt tctccggagt gttggcggaa
     1141 agtgtcggcg gtttgggcgt tagcggcacg gctgcgggcg taatcctcgt gcttgcttat
     1201 atgtatgcgc attatatgtt tgccagtact actgcacata ttaccgctat gttcggcgca
     1261 tttctcgctg ctgccgtttc actgaatgcc ccggcgatgc cgaccgcgct gatgatggcg
     1321 gccgcatcca acattatgat gaccctcact cattatgcga ccggtacttc acctgtgatt
     1381 ttcggctcgg gctacaccac aatgggagaa tggtggaagg cgggttttgt catgagcgta
     1441 gtcaattttc tgattttttc cgttatcggc agcatttggt ggaaagttct gggatattgg
     1501 taaagggaaa aataaatttc caatctgtgt ttatttgatt gggcgactat tatcgtgaaa
     1561 tatgccgtct aaagccttca gacggcatat ttgtgcgctt ggatgttgca gaaagcggca
     1621 gggcggcggt gtaggaaaag ccaaacaaaa accaaaccgc ctgtcaactt ctgataaaca
     1681 taagtattaa ataatcagaa ggttattcaa ttacctaaac gcaaattccc ctgccgtatc
     1741 acatctattg aaaataatac atcaaccggc tcggaagcag cctgatcagg tgattctact
     1801 tgcggcgatg aatcggcagc cggttcggta taggcagtcg gcgtgccgtc ggattgggta
     1861 tttaaatgcg cttgtgcctc gggcgtgtgt acatcaggca cttcgggcgt gcgtgtctcg
     1921 gatacttcgg cagagttggt ttcctcagtt tgttcaatga cttcagcttg gctgtatgag
     1981 gaagaaccct gtatccacgc cagcgatttg agcggcatct tcatcttgcc gtttttgccg
     2041 caggtcagac agacggccga tccggtgcgg tctttgagta cggcatcgac tttctcgggc
     2101 gcgatggttt tgccctgtgt ttttgcccat tgcccgatgc tttttttcag ggaggcggtg
     2161 tcaaggttgt tcttaccgta agggtcgcgg aaggcggcaa gccacaggtt gcgatccgca
     2221 tcttcattga ggcccgccat ttgatagatg acggaggcgg gcgagccggt ggcgatggtt
     2281 ttggcaaact cgagcgcgtc gggcgatttc atgcggacgc agccgtgact ccggatgccc
     2341 gggacgctgg ccggtgcatt ggttccgtgt atgcccaaac cgagtttcgg gtctcctaag
     2401 cggacaaaaa ccggccccaa agggttgtcc gggccggcgg ctatggtttt tacgccgtcg
     2461 ccgcgttctt tctgtatgga ttttgggatg tgccaaacag ggttataggc tttcgccccg
     2521 attttatgtt cgcctagatt ggtttgtgtc atcgcccgac ctactgcaac gggataaacc
     2581 ttggtcagtt tgccgtcggt gtagaggaac aggcgttgct gagggatgtt aatgaagaca
     2641 tgttgacctt gtgcgacggg ggagacatcg ggaatgacgg tgtttgcgta tgaaaaaccg
     2701 cttatcaata gtgcggcagt gcggcagatt gttttattca tatcaaaata tggtgtgtgt
     2761 ccgataggtt ttcggcaaat catacctgaa accgtaccaa tttgtgcgaa aatatgcgct
     2821 tcggtacagt gcggacggat tgggtaatgg caacggaaac aaatatcgcg gaaatttccg
     2881 ccttggatta tgaaggcagg ggtgtggcaa aggtcggcgg caaaacgatt ttcattaaag
     2941 gggcattact tgattgtttg atgctgggtt ggttcaggct ttaactcagg aatatttaca
     3001 tcataatgaa agtttttaga caacagcttg aacaactcgg cgcgcaaaat caatatcgtt
     3061 cgattccgga tttgattcat caagggcggt atattacgcg ggaaaaccgc aaaatgctga
     3121 atatgtcgtc taatgattat ttggggttgg catcagatga aaacttacgc cggtcttttt
     3181 tgcagcaata cggcggtaat tttccctctt ttaccagttc ttcatcacgt ttattaacgg
     3241 gcaactttcc tatttatacc gatttggaag agcttgtcgc acaacgtttc caacgggaaa
     3301 gcgcgttatt gttcaacagc ggctatcacg ccaatctcgg tattttgcct gctttgacga
     3361 cgacgaaaag tttgattttg gcagataaat tcgttcacgc cagtatgatt gacggcatcc
     3421 gtttgagccg gtgtgcgttt ttccgttatc gtcataatga ttatgaacat ttgaaaaatc
     3481 tgcttgaaaa aaacgtcgga aaatttgacc gcacttttat cgttaccgaa tctgttttca
     3541 gtatggacgg cgatgtggcg gatttgaaac agcttgtcca attaaaaaaa cagtttccca
     3601 atacttatct ttatgtggat gaagcccacg caatcggtgt ttatgggcaa aacggattgg
     3661 ggattgccga acgggataat ctgattgccg agattgattt attggtcggc actttcggta
     3721 aagccttagc ctcggtgggg gcgtatgccg tctgcaacca agtattgaaa gaatgtttga
     3781 ttaatcaaat gcgcccattg attttttcaa ccgcattgcc gccgtttaat gtggcttgga
     3841 cttattttat ttttgaacga ttgccgcaat tctcaaaaga aagaagtcat cttgagcagt
     3901 taagcgcatt tttacggcgg gaagtggcgc atcggacgca aataatgccg agcgaaacct
     3961 gtatcgtccc ctatatttta ggcgggaatg aagccaccct tgccaaagcg gaatatctgc
     4021 aaggtcaggg ttattattgc ctgcccatca gaccgccgac agtaccaaaa aacacatcca
     4081 gaatccgcct gtctttaact gccgatatga caacggatga agttcggcag tttgcggcgt
     4141 gcctgtaagg atatgatatg aaaacaaaat tttacaatca tcaaggcgaa catttaatcc
     4201 tgtattttgc aggttgggga atgccgcccg atgctgtaaa tcatttgatt ttgccggaaa
     4261 atcacgattt attgatttgc tatgattatc aagatttaaa tttggatttt gatttttccg
     4321 cctatcggca catccgtttg gtggcgtggt caatgggcgt ttgggcggca gagagggcat
     4381 tgcaaggaat aagattaaaa tccgcaacgg cagtgaatgg cacaggtttg ccttgcgatg
     4441 ataatttcgg tatcccttgc gccgttttta aaggcacatt ggagaatctc acagaaaata
     4501 ctcgttcaaa gtttgaacgt agaatctgtg gcgataaagc atcttttgaa gattaccaac
     4561 aatttcccgc ccgtccgttt gacgaaattc atcaagaact taccgcactt tttgcgatga
     4621 tcgggcaaga taggcgtaca gatcttatcc gctggacaaa cgccttgttc ggatcgggcg
     4681 ataaaatttt tattcctgcc aatcagcacc gatattggac accgcgttgc accgttcaag
     4741 aaacagacgg cgggcattac ctgttttcaa gatttaccca ttggtcggca ctatggaatc
     4801 actgactgcc ataaataaac cgcgcattcg gcaggctttc caaaaagcat taagcgatta
     4861 tgacaggcac gccttaatcc aacaaaaaat gacgattaat ctaatcgcgc atttgcaaga
     4921 ttatttgccg gatatgccat tggaaaacgt gttggaattg ggctgcggct caggaatgtt
     4981 gagtgccttg ctgcaaaaac agatttcagc gaattattgg ttatttaatg atttgtgcga
     5041 tgtacgaagc cgactggctg aaaaattgcc tcaatccttt gatttttatt gcggcgatgc
     5101 ggaaaacttt ccttttcaac gacaatttga cttaatcgca agcgcatctg ccgtgcaatg
     5161 gtttcaccaa cccgacgctt ttatcgccca ttgcaaaaca ggcttgaaaa caaacggatt
     5221 attggcggtt gcgacctttg gcaaagacaa tttaaaagaa gtccgccaaa tcacaaatat
     5281 aggcttaaat tacccgactt tatcccaatg gcaggcttgg ttagccaaag attttgagct
     5341 tttatggtgt gaggatttta aggtaatact agactttgat acgccgttag atgtactcaa
     5401 acaccttaaa tatacaggcg ttaccgccac gaaccaaaaa aattggacaa gaaaaaatct
     5461 caatggattt attggcgatt acttgtcggc gttcggtatg ccgtcgggca aagtgcgcct
     5521 gacttatcat ccgttatttt ttatcgcgcg ccactctgcg gcgggcaggc aataaggcag
     5581 cttatgggca aagttatttt tatatcgggt attgatactg atgtgggtaa aaggtaatat
     5641 ggcgagcctt atgcagaagg catattgtca aacgttaaat tatggtatga tttaaaattt
     5701 acaagtctat ttcagtaaat cgttaataat aaaagcggac aatttaataa taaaagcgga
     5761 taattggccg ttgcaggcgg tcagctatcc gctcgttacg gtatcggggg tttcctttgg
     5821 aaaactcatt aattatcggt ctggcactgg cggtattgct gatgcttttg gttatgcgtg
     5881 cgaagcaggg taagaaaaca ccgaagcgca aatcccaagg tgtcggcaat acgcaggggc
     5941 agccgcccgg aagcaatgat tcggactggg tcgatcagac ttggtcaatc ggtttcagac
     6001 ggcacgcaac tcgactggtc ttggaacgaa agtgccgaga ccgcatccgc cgccgtatcc
     6061 gcgcaagaag tcgatccgct tacggagtat caggtttata agcaattcgg ttatcagggc
     6121 aaggctgccg aatctttggc tgcctatctg gacggcattc cggatggtga agcgaaacct
     6181 gagaacctta tccgcgagct gatcgatatc aatctcgaag tgggggatgt cgatgttttg
     6241 gcagacaatc tgcaaaaata cggcagactg attcctttcg aacttttggc aaaatatatc
     6301 gaacaggcat tacagcgaga ttcaaaccat ttgcgtatcc gcgtcttggc ggaagaaggt
     6361 ttgggatagg gcactcagga gattgaaaaa cgtgcggaag gcggttctgc gacggcagct
     6421 tccgcatcgc ccccgccgga tgtcggcggt aaggcttatg gagccgaaga aatcaagcgc
     6481 atcccgattg tgcggggcaa aaaagacgtg tccggaatca gccaagagaa aatcggtgcg
     6541 attgccggcc tggtccgcgc cgatcaaggt gcgaaaatcc ttaaagacaa agtcagctac
     6601 gaaacggcat cgaaacaata cgaccgtgcc atccaaactt ccgaaaaacc tgcaaacctg
     6661 attatcgatg cgttgaaact cgattaccaa cacgcggaca tagaccgttt tgccggacat
     6721 ttgtggaaac tttaccaaac gttgggcaac tacggcaggc aggttaaaga gcggatgctg
     6781 ggttaggggt acagcctggg ttaccatgaa gttttcgatg atttggaaaa gggggcgaac
     6841 gatcggcaaa tcaaagacat cggtatgagg cacgggtatc tgccgaaaaa tatacagaaa
     6901 ttcaaagcgc aacatcggga tttggtgctt caagattctt cgttgatcaa cactggttcg
     6961 tctccggcag acgatgcggt taaagaagta gagtcgttgc tgatgtatgg tcagattgaa
     7021 gcggcaatgg atgtgttgga gcaggcggta ttgaaatatc ccgacgaatc ccagctttat
     7081 attacgttga tcgatattta tgaacgtact gaagattggg ataggttggg gcagttttta
     7141 agggtattga gggaacgtgc ggacaggctt cctgaagagg tcgttatgct gatgagccgg
     7201 ctgctgcagc gtatgaatca aaatattaaa aaaataaaac ggtacggaaa ataaaaatgg
     7261 aagttcaact gccgaaaatt aaaacagtac gcgtaatgtt ggcggggatg acggcgcagc
     7321 aggaatccgt tttcaaaatg gcattcaaaa tgcacaatac cacccgttat gaaacagtat
     7381 ccccttcaga cggcagtgcc gtgcccgatt tggttttggc ggataccgat gccgagggcg
     7441 gttttgaact ttggaaagag cttgccgggc gttataaggc tatacccgtc gccgtctgtt
     7501 cggagaaagt tcccgattct gaagttccct acctgcccaa accgattcgg tttgaaacat
     7561 tgtttcctat gctccgcaag ttattgcagg gcgagaatgt ttatgggaaa tcgtttattg
     7621 cacccgcaga ccggtcggcg aaaaacaacg ggaatgtgca gcgtacggtt acgatacgcc
     7681 agtttaaccc gaataaagga ttattggggg cgttgcggtt tgcggaaaag aacaggcagg
     7741 acatcgctat cttgcatgga aataagccgg tccttaattg ttttcccctc gatacaacgg
     7801 gttttgctga cagaaagtgt gtaaaaactc gaagaattgt gcaaagacga aaatttgcag
     7861 gtcagttgca agactgttcc cgataactcg caatggcgcg aaaaggctaa agtaggcatt
     7921 atgtcctgta tgtggcagtt ttccatttgg acggcgcagg acaggttgat ttatccgatt
     7981 tttcccgata ctccgtttac gttgaaatct tggccaaacc tgacccggta ggcaaatgtg
     8041 ccggggtcga tacgcttgtc ggcatttttg accaaggcat ccgtcaacct taacgtgttg
     8101 tataaagtga tgcctttaaa cctcaatgat attctgaatt atcttgcggc aacctataca
     8161 accgggtttt tgtcggtaga tttgaaaacg gtttcacaac aggcatactc cgatatggcg
     8221 gataaaataa atatcggggc cgattctgct tctgatagtg aaatgatgaa aaaagcggaa
     8281 aaaatcacga caccatccca atcccagtcg cgcggccttc tgcaaagact gatgaaaaaa
     8341 ctgttgggca gctaagaggc ggagagatga gagaaaataa aattattttc acaggacctg
     8401 tcggcgtagg gaaaaccact gccattgcgg ctatttcgga cgaagcactc gtccagaccg
     8461 atgcttccgc atccgacatg actttggata ggaaaaggaa tacgacagtg gcgatggact
     8521 acgggccatc agcttggatg aggataccaa agtccattta tatagtacgc ccggtcagga
     8581 acggttcaac tttatgtggg aaatcttaag ccaaggcagt atgggtttgg tattgctttt
     8641 agataatgcc cgaaccaact cgttgaaaga tttggaattc tttttacatt cgtttcgagg
     8701 gctgctggag aaggcatccg tcgttgtcgg tattaccaag atggatatac gctctcagcc
     8761 cggtatcgac gtgtatcaca aatatcttgc aaaacataat cttaatgttc cggtttttga
     8821 aattgatgcc cgtaaggaag atgacgtaaa acaattggtt agcgcaatgt tattttctat
     8881 tgatccggga ctggaggttt aatatggaat caacactttc actacaagcg aatttatatc
     8941 cctgcctgac tcctgccggt gcattttatg ccgtatccag cgatgccccc agtgccggta
     9001 aaactttgtt gcgcagcctg ttgaaagcgg atgcggacga agtggtcagc agtgagaagc
     9061 tgctcgcggg cggacaccgc cgacatcgat accgctttga acctgttgta ccgtttgcaa
     9121 aaactcgaat tcctctatgg cgatgaaaac ggtcattcag acggcatcaa tttgtcggac
     9181 gagcaattgc cgttgctgat ggaacaattg tccggcagcg gtaaggcatt attggtcgat
     9241 cggaacggtc tgtatcttgc caacgccaat ttccatcatg agtcggcgga agagttgggg
     9301 ttgttggcgg cagaagtcgc acagatggaa aagaaatacc ggctgctgat taggaacaac
     9361 ctgtatatca acaataacgc ttggggcgtt tgcgatcctt ccggtcagag cgaattgaca
     9421 tttttcccat tgtatatcgg ttcaaccaaa tttattttgg ttatcgccgg cattcccgat
     9481 ttgagcaaag aggcatttgt tactttggta aggattttat accgccgtta cagcaaccgc
     9541 gtgtaaaact tgggacagga gggattatgc agcaattatt gatttcaatc cttgaagatt
     9601 taaataatac atctacggat attatcgcgt ctgccgttat ctcaaccgac ggattgccga
     9661 tggcgacaat gcttccttca catttgaatt cggacagggt aggggcgatt tctgccactt
     9721 tgcttgcttt ggggagtcgc tcggtgcagg aactcgcctg cggggaattg gaacaagtga
     9781 tgattaaagg aaaatcaggc tatatccttt taagtcaggc gggtaaagat gccgtgttgg
     9841 tgctggtggc aaaagaaacc ggcagacttg gtttaatcct attggatgcc aaacgtgcgg
     9901 caaggcatat tgcggaagcc atataacata taaagattgc gggcttgcag ataagtgcaa
     9961 tcgattgtca atttatattg acacgttcgg tatttctgtt ttattattcg cgtttactcc
    10021 ccgatagctc agtcggtaga gcgacggact gttaa
//
LOCUS       DAWTHD010000085        10038 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWTHD010000085 DAWTHD010000000
VERSION     DAWTHD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.085"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..258
                     /locus_tag="WM704_001643"
     CDS             <1..258
                     /locus_tag="WM704_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HFA9341018.1"
                     /translation="NQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAESKIAEL
                     TNKIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            239..694
                     /locus_tag="WM704_001644"
     CDS             239..694
                     /locus_tag="WM704_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341019.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            701..757
                     /locus_tag="WM704_001645"
                     /pseudo
     CDS             701..757
                     /locus_tag="WM704_001645"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            763..1140
                     /locus_tag="WM704_001646"
     CDS             763..1140
                     /locus_tag="WM704_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9341020.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFTIKSSPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(1204..3417)
                     /locus_tag="WM704_001647"
     CDS             complement(1204..3417)
                     /locus_tag="WM704_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFA9341021.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATSEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            3560..4201
                     /locus_tag="WM704_001648"
     CDS             3560..4201
                     /locus_tag="WM704_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFA9341022.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            complement(4273..4911)
                     /locus_tag="WM704_001649"
     CDS             complement(4273..4911)
                     /locus_tag="WM704_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFA9341023.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            5167..6018
                     /locus_tag="WM704_001650"
     CDS             5167..6018
                     /locus_tag="WM704_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFA9341024.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            6084..6746
                     /gene="exbB"
                     /locus_tag="WM704_001651"
     CDS             6084..6746
                     /gene="exbB"
                     /locus_tag="WM704_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFA9341025.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            6749..7183
                     /locus_tag="WM704_001652"
     CDS             6749..7183
                     /locus_tag="WM704_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HFA9341026.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(7684..8112)
                     /locus_tag="WM704_001653"
     CDS             complement(7684..8112)
                     /locus_tag="WM704_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HFA9341027.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(8177..9925)
                     /locus_tag="WM704_001654"
     CDS             complement(8177..9925)
                     /locus_tag="WM704_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFA9341028.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
BASE COUNT         2433 a         2254 c         2885 g         2466 t
ORIGIN      
        1 aatcaggcgg aaacggaagg ggtttgggtt cataattctt gtccgcctaa aagaacagga
       61 agctccaaga atgaaaaaca tggagacggc ggtcgaagtc aaatatcagc ggaatcaaaa
      121 attgctgaat taacaaataa aattattccc ggaatgtcca aaaatgaacg cttaaagatt
      181 aagcaaaaaa tcaaaaatat tgccaaaaat gccaatcgaa aaacaaaagg agaagagcat
      241 ggtcgacgcg gtcgttaaaa ctcccgaatt tttacctttt acttctgtag gaatattccg
      301 ttttggtgct gatataacac agtacaaaaa catactggaa acttttatgt atgaacctcc
      361 tgatgagttt ggaacagaat attatgaatc tcccgactca aatttactca tttccgtcaa
      421 aaaaaacaaa attatttcga tattttgtta tcaggagcta tattttatgg gcgttaatat
      481 aataggtctg aattttgaag attttaaaca gcttttccat caccctaaat catacggtgt
      541 tgataaatat tatttatcaa atgaatctta tccgacatat gtctatgagt ttgatgaaat
      601 aggagtacaa gcatgggaag caaaagggaa aattgttact atcattgccg ggggcaaaga
      661 caattattcg acagaacctt actacgatga ataacattac agaaaagaaa caagaaacgg
      721 tacatttgat aaaaacctaa atcgtattgg agattaatta ttatgtgcga gttcaaggat
      781 tttagaagaa acatcccttg ttttgaagag tatgacgaaa attcatttat tggtaaatgg
      841 tatgatgacg gggtgtggga tgatgaagaa tattggaagc tggagaatga tttaatcgag
      901 gttaggagaa aatatcctta tccgatggat ataccaaggg atatcgtgat tggaatcggt
      961 accattattg agtttttaat ggttccaaat tggaaacttt ttacaattaa atcttcccct
     1021 tggttgcctg atagtgtggg aattcatgaa cgttatgaaa gattcacaac gatgctccgt
     1081 tatattttta ccgagaaaga catagtcaac gtgcgatttg attattacaa caaaaaatag
     1141 caaaacccgg caatcaaaat gccgtctgaa gcgttatccg gctttcagac ggcatttttg
     1201 tatttaaagc cgggtaacgc tcaatacgcc tttgacatcg ccgaggccgg cgaggacgcg
     1261 cgggaggtcg ttgacttgtt tgacttcgag cgtgaacctc atgctggctt ccaagtcgcg
     1321 ggactgggtt tgtacggcgg taacgttgag tttgtggcgg gcgagcgcgt cggatacgtc
     1381 gcgcaaaagc ccggagcggt cttgggcgcg gatttcgata tcgacggcga acacttgccc
     1441 ttcctgcaac gccgcccaac ttgcgtccag tactttttcg ggcgcgtgtt cggcaaggtg
     1501 tcggaaagag gggcaggttt tgcggtggac ggaaatgccg cgctcgcggg taacgaagcc
     1561 ggcaatatcg tcgggcggcg cgggtttgca gcatttggca agcgtggtca tcaagccgtc
     1621 ttcgccgtcg atgagcacgc cggttttgcc accttttttg attttggact gtttgacgat
     1681 ggtggttgcg ctgacgggca cgggcggcgg ttcgttcagc gtgccgcagg ctttttggat
     1741 ggcgcggttg gaaatttcgc cttgtccgac ggcggtgtag aggtcttctg gctttttgta
     1801 gccgagattt tcggcaagct cttgcaggtt gggtttgggc gtgagtttgg caagctgctt
     1861 gtcgagttgg acacggcctt cttcgcgcac ggtgtcggcg ttttgctggc ggatgtaggc
     1921 gcggattttg ccgatggcct tgccggattt gacccagcct tcgtaaagcc agttgacgga
     1981 aggatgccct tctttggcgg taatgatttc gacgcgctgt ccgttttcga gcggggtgga
     2041 cagcggcaca atctgccctt cgactttcgc gccccggcag cggtcgccga tgctgctgtg
     2101 cagggcgtag gcgaagtcga tgggggttgc gcccgttggc agagagagga ctttgccgtg
     2161 cggggtcaaa acataaatcg tgtcgttgaa aagctcggtt ttgaaggcgg cggcgaggtc
     2221 ttccttgccg ctttccgcca tattttcgcg ccagtccaag agttggcgca accaggcgat
     2281 tttttgttcg taggcggaat cgcctttgcc gccttctttg taacgccagt gggcggcgac
     2341 accgaattcg ttgaattggt gcatatcgaa ggtgcggatt tgcacttcca cacctttgtc
     2401 ttccgggccg acgatgacgg tgtgcaaact tttataaccg ttgcctttgg ggttggcgat
     2461 gtagtcgtcg aactcgccgg gaatgggctg ccagaggctg tggacgatgc ccagcgtggt
     2521 gtaacactcg gggacggtat cgaccagaat ccgcacggcg cggatgtcga acaggccgtc
     2581 gaagctgagt tttttcttca ccattttttt gtaaatggag tagatgtgtt tcggacggcc
     2641 ggcgacttca aagtggatat tgtatttttt gagttccgta cgcaggatat cgaggaagtt
     2701 ttcgatgtat tcgaggcgtt cggtgcgttt ttcgtccaaa agcagggcga tttcgcggta
     2761 tttttcgggt tcttgatggc ggaagcccaa atcttcgagc tgccatttga gctgccacac
     2821 gcccaagcgg ttggcgagcg gggcgaagat gtcgagggtt tctttggcga cggcgcgttt
     2881 ttcggggctg tcgggggcgt tgcttaaaaa ttgcagggtg cgcgtacgca tcgccagttt
     2941 gattaatacg acgcggatgt cggtaaccat cgccagcagc attttccgca tggtttcggc
     3001 ttgctgcgcg cgctcttcgg aagtggcgag gctgtccacc cgggcgaagt gggtgagctt
     3061 ctgcacttcg tccacacctt tgaccagctc ggcgacggtg ctgttgcagc gctcggaaac
     3121 caataggttc caatcgggga cgtagcgtcc gatgtcggca agcagggtgg cggcgacggc
     3181 atcgggcagc aggtcgagtt cgtcgaccat ttgcgccgcg ccgaggaagt ggtcgggcag
     3241 cggctcgcca tacggcgtgg cggcatcggc aggataatgt tcctgcgcca gcgaccatgc
     3301 ggtaccgatg aggtttttat cgttgtccgg cagagcggcg cagtagctgt cgaaccattc
     3361 gcgcaattct tgcagggtcg cgctttgcgt gtcttgaatc gggctgatgg cggtcatgat
     3421 gggatcttcc gttccgataa aaacgagttc attgtaacag cttatttatt tacggtgcat
     3481 aggcgggaag ataaacttgt ttcgggcggc gccgggtgtt aaggtagccg gtaccgacag
     3541 gttgacgagg aatattttga tgaaactgta tatttacgac cattgcccgt tttgcgtgcg
     3601 tgcgcggatg gctgccgggc tgtttggtgc tgacgttgaa gaagtcgtgc tggcaaacga
     3661 cgacgaggca acgccgatcg gtatgatcgg tgcgaaacaa gtgcccgtcc tgcaaaaaga
     3721 agacggttcg tttatgggcg agagtttgga cattgtgtgc catttcgacc gagaaggccg
     3781 tctgaaagac gaagtccgcc ccgagataca ggcgtggctg gacaaggtag gcggatacaa
     3841 caacaaattg gttcagccac gcttgatcaa aatcgggctg cccgaatttg taacgccgga
     3901 agcggtgaaa tatttcacag acaaaaaaga gaaaagcatc ggcagctttt ccgccaattt
     3961 gaataaaacc gcccaatacc ttgaacggat aaacgcagat ttgcaagagt tggagaattt
     4021 gatggacggt acttcagacg gcatcaacgg tggaatcggt atggaggata ttttggtttt
     4081 ccccgttttg aggaatctga ccgtggtgcg cggcatcgcg ttccctcgga agacgatgga
     4141 ttatttaatc ggtatgagtg aaaaatcagg cgtgccgctt tatttcgaca gggcattgta
     4201 aaaccaaaaa aaagcggcag ggcaatatgt ccttccgcgt tttttggaac gtttatgccg
     4261 tctgagagcc agtcagacga acatctgcac aaacgcaatc accagccgca caatcggtgc
     4321 aataaacgca cccaaaaccc cggtcagcat aagcagcagg ataatccacg tcccataagg
     4381 ttcgattttg cggaacgctt gcgaatattt cgccggcagg aaggtgtcga tgaaaatgcc
     4441 gccgtcccaa ggcaggatgg ggatgatgtt gagcgcgaac aaaatcgcat tgatcagaat
     4501 accgtagttt gccatttgag ccagcggcat ctgatacgcc ccgccggcat acggagttag
     4561 caccaaaacc acgccccaca gaacagccat cgccagattc gacagcgggc cggacgcggc
     4621 aatgcaacgc catgcaaggc gcgggttgcg gaagttgcgc gaatcgatag gaatcggacg
     4681 cgcccagccg aacaggaagg gcgtgaacat caaagtaagc agcggtacga tgattgtgcc
     4741 gaccaaatcg atatggggca gggggttcag tgtcagcctg ccgtattgtt cggcagtgtt
     4801 gtctccccag tagcgcgccg tatagccgcg cgccacctcc ctgacggtaa tggcgagcag
     4861 cacgggcagg acagcaagca gaaacacgcc caagtcaaaa ttttgaaaca tggttttcct
     4921 ttgaacagat gtggtgtgca gcccgaaacg gctgacagac agaagcggca atatatcatc
     4981 taacctgatt ttattggaat aagaaatcag acggaatcat tttttctgcc ggcccgttat
     5041 ctgatgattt gctgtaatac cgcgtttaaa cacggtttgc ctatgaatgg agcaggcgtt
     5101 cacatgacaa caaattataa aaaataggtt gcaaatagga attgttgcta ttattatgcc
     5161 cgaattatgg ataaagaacg aattttaacc cccgcagtcg tgttttccgt cgcactgctg
     5221 catttggcaa tagtggcatt gctctggcag gcgcacaagc tgcccgtgat agagtcagga
     5281 aatgttatcg aatttgtcga tttgggcgat tttggcggag ggggcggcgc acccgaaggc
     5341 gcaggcgcgc ctgccgcgcc cgaaccgcaa cccgcgcccg atccgcccaa acctgtcgaa
     5401 ccgcccaagc cggttttgaa gccggcggtt acaaaaaagg cggatgcgga tattcagcaa
     5461 cctaaggaaa agccgaaacc cgaagaaaaa cctaagcccg aacccgaacc ggaagcgaag
     5521 cctgccccga aaccggcgga aaaaccggca gagaagccgt ctgaaaaacc tgccgaacat
     5581 tccggtaatg cttctgccaa agccggcagc gagcagggta atggggaagg taagggaacc
     5641 ggtaccaaag gcgacggaac ggggcgcgga gaaggcagcg gtaaaggaag cggcggtgcc
     5701 aaaggcgaac acggggaagg agccggcggc agcggcggcg gaaccggtgt cggcagcagc
     5761 aaaggcaatc ctttacgcgc caacggcagc atcccgcgcc cggcctatcc cgcactttct
     5821 atggaaaatg acgagcaggg tatggttgtt ttgagcgttt tggtttctcc gggcggtcat
     5881 gtcgagtccg tcaaagtggt gaaaagcagc ggtttttccc gtttggacaa tgcggcacgc
     5941 aaagcggcgc aaaacgggca ttttcaagcc aatgcctgga cggagtttaa agtccccgtc
     6001 aagtttgaat tgaattaggc ggttgtcgcg gcagtttgcg gcagcttgga tgtttgattg
     6061 gttttttcag tgttggaatc attatgaatt tgaaattagt gtttgaatcg ggcgatcccg
     6121 tcctgatcgg tgtgtttgtg ttgatgctgt tgatgagtac cgtaacgtgg tgtctggttg
     6181 tcttgcgctg catcaagctg tatcgggcgc gcaaagggaa tgccgccgtc aaacggcata
     6241 tgcgcgatac tttgtcgctg aacgacgcgg tcgaaaaagt gcgcgccgtc gatgcgcctt
     6301 tgtccaaact ggcgcaagag gcattgcagt cttaccgcaa ctaccgccga aacgaagcgt
     6361 ccgaactggc gcaggctttg ccgttgaacg agtatttggt cattcaaatc cgcaacagta
     6421 tggcgcagat tatgcgccgg tttgattacg ggatgaccgc gcttgcctcc atcggcgcga
     6481 ccgcgccgtt tatcgggttg ttcggcacgg tttgggggat ttaccacgcc ctgatcaata
     6541 tcgggcaaag cgggcagatg agtattgcgg cggttgccgg cccgattggc gaggcactgg
     6601 tggcgacggc ggcgggtttg ttcgtggcga ttccggcggt gttggcatac aacttcctca
     6661 atcgcggcac aaaaatactg acccaggatt tggatgcgat ggcgcacgat ttgcacgtcc
     6721 gcctgcttaa tcaaaaggat agctgattat ggcatttggt tcgatgaatt ccagcgacga
     6781 ttctccgatg tccgacatca acgttacgcc gttggtggac gtgatgctgg tgttgctgat
     6841 tgtgtttatg attactatgc cggtgctgac gcattccatc cctttggaac tgccgaccgc
     6901 gtccgagcag gcaaacaagc aggacaaaca gcctaaagac cccctgcgcc tgacgattga
     6961 tgcgaacggc ggctattatg tcggcgggga ttctgcaagc aaagtggaaa tcggggaagt
     7021 ggaaagccgt ctgaaagccg ccaaggagca gaatgaaaac gtgattgtgg cgattgcggc
     7081 agacaaggcg gtggaatacg attatgtaaa caaagcttta gaagccgccc gtcaggcagg
     7141 aattaccaaa atcggttttg taaccgaaac caaggcgcaa taaagttcga cgcttgaacg
     7201 aatgccgcct gaaagtcttt cagacggcat ttcttatgtt taatcagtta tatatagttg
     7261 attaaaacaa aaatgagacg acagagcacc gtctccattt ctgcgcaggc gggaatacaa
     7321 acatctgatt tttcttttat tttcaaacat tactgaaatg ggtaagtctg gagtcctgcc
     7381 tgcgcgagaa taatgaaaat gtgcatttct aatttttata gtggattaac aaaaatcagg
     7441 acaaggcaac gaagccgtag acagtacaaa tagtacggaa ccgattcact tggtgcttca
     7501 gcaccttaga gaatcgttct ctttgagtta aggcgaggca acgccgtact ggtttttgtt
     7561 aatccgctat acccactgta aaaacggaga aggacagagg acggcagtaa ggtttgccgt
     7621 cctctttttc aagttgcggt ggaatgccgc ttgaaggtgt ggtttaaacg tttcggcttg
     7681 taatcagacg cgggcgttgt ccagccgctc tctgaagttt tccagattca cacctgccgc
     7741 ttgggcgcgg cggatgattt cgtcaaccgg cataccttct gccgcccggc ggaagcccca
     7801 caggagggag cagcgcgtac cggtccggca ataggcaagg acgggatatt cggcttgtcc
     7861 gatgagttgg cggaaggttt cgacatcgtg tttttggatg tcgcgtgcgg taacgggttg
     7921 gtgatggaat ccggtaacgc ctgcttgttc cagccactgt ttgatttggg cgaagtcggg
     7981 ttgcgattct tcttcgcggt cggggcggtt gcagatgacg gttttgatgc caagttgcgc
     8041 gatttgttcc gcatcggctt tggtcagttg cggggagatg tagagatgtt cgtcaagttt
     8101 gagaatcgcc attttgtttc ctttgctttg cggttggtgt tcagacggca ttttgtgtgc
     8161 aatgccgtct gaaagtttaa ccgaacaacc agttttccaa caggttgcga tctgtgatgt
     8221 gtaggatggc agtatccgcc gccgttgcct gaaccgtcaa gacggtttga cgcaatatgc
     8281 ctgcacggaa gaagtggata ttgatttttg ccctgacgtg gtatcgggcc cattgtgcgg
     8341 caaagtcggt gcaggcataa ccgtctaaag caatgatttt gtcttgcggg cacagtgccg
     8401 cagattccgc acttccgccg ttgaagacat gggtcaggac gatatggtcg gcgttttgtt
     8461 tgaagcgtgc gccgaaatcg cccgtcggcg ggatggggaa aatgtgttcc gcatatccgc
     8521 cgccgtgttg tcggggaagc ggcaggaagg tcagtttcac gcctgtggtt gccaggcatt
     8581 cggcaagcgg caaatcttcg gtgctgtaca atgccgtctg aaaaaaatct gtcaaatcca
     8641 agccggtaat ttcctgacaa cggatttgcc agtgtttttc cggaataccc gagtgtgtgt
     8701 ccctccactc ccgatagagt ttgtccatta ccgtatcaag ggaatgtctg ccgttgcttc
     8761 ggttgcgtat tatcagatca aggcacaatg cggcaagtgc gcctttttgg tagtagctga
     8821 cgatggcgtt ggggctgttt tcatccggtt tgtaaaattt gttccacgcg gtaaaactcg
     8881 attccgccaa ggtctgcctc aaacggccgc gggtttgttg tacgcgcgta atgccttgtg
     8941 ctagcaggtt taaataaaat tcgggcgaga tggtgcggct gcgtgccaaa aacaaatcgt
     9001 cgtaatagga tgtaatgcct tcgaatgccc atagttgttc ggtatagttt tctttgtcga
     9061 ggtcataagg ggcgaatgcg gcaggtttga tggatttgac gttccacgcg tgaaaatatt
     9121 cgtgggagaa aagtccgagc aatgtggtgt aggcatcgtc ggcatcggtc ataccgtacg
     9181 gcggaaggct gtggcgatcg gcgagcagtg cggtgctgtc ggtgtgttcc aaaccgccgt
     9241 aaatatggtc gccgacgtgg agcaggaaca aatatttctc aaatggggca ggggaggaga
     9301 acatcgccag ttctgtttcg cagatttttt tgatgtccga aaccagcctg tcgcggtcga
     9361 aatcgggata tatgccgttt aaggcaatcg tgtgcggaat gcctgccgcc tcaaaatcta
     9421 aaaattcaat caagcccatc tcgacaggtc ggtcaatcaa ttcgtcataa gatgccgtct
     9481 gaaagacaag cctccctgtt tccggcagcg ttgtggcaat acgccattcg ggtggaatgc
     9541 ctgtcaattc caagcggtgc ggcagcgttt ccgttccttc gactttcaaa aacaggcacg
     9601 atccgtcaaa aaaaccgcgt tctgtcgtca ggaaagaacc tcgaaccgac aaatcgaatg
     9661 catatacggt gtagcggatt tgccactcgc cgcgtacggc ggcagtgtgc cagcggtttt
     9721 ttgcaatttg ttcgagcggc atggacgtgc cgttgcagga tgcatggata gaagtgatgt
     9781 ggcgggaaaa atcccgaatc agatagctgc ccggcaccca attcggaagg cttatttcta
     9841 taggaaaatc attatcttgt gtgaatttca gcaggatgtg ccattcatgg tctagagggg
     9901 acggggctat tttataatga atcataacta tcaaatatat cgttaaacgg gatggattaa
     9961 atgcctttaa aatgcagctt aacctacatc aataaagtgc cacaaaaagg gaaaattgga
    10021 aacaaaagca atggcggc
//
LOCUS       DAWTHD010000086         9868 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWTHD010000086 DAWTHD010000000
VERSION     DAWTHD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.086"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            398..961
                     /locus_tag="WM704_001655"
     CDS             398..961
                     /locus_tag="WM704_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFA9341029.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            964..1512
                     /locus_tag="WM704_001656"
     CDS             964..1512
                     /locus_tag="WM704_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341030.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1519..4734
                     /gene="carB"
                     /locus_tag="WM704_001657"
     CDS             1519..4734
                     /gene="carB"
                     /locus_tag="WM704_001657"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFA9341031.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(4935..5975)
                     /gene="queA"
                     /locus_tag="WM704_001658"
     CDS             complement(4935..5975)
                     /gene="queA"
                     /locus_tag="WM704_001658"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFA9341032.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            6092..6349
                     /locus_tag="WM704_001659"
     CDS             6092..6349
                     /locus_tag="WM704_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFA9341033.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6331..6792
                     /locus_tag="WM704_001660"
     CDS             6331..6792
                     /locus_tag="WM704_001660"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFA9341034.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6906..8267
                     /gene="accC"
                     /locus_tag="WM704_001661"
     CDS             6906..8267
                     /gene="accC"
                     /locus_tag="WM704_001661"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFA9341035.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTFADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIPDKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            8371..9258
                     /gene="prmA"
                     /locus_tag="WM704_001662"
     CDS             8371..9258
                     /gene="prmA"
                     /locus_tag="WM704_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFA9341036.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            9276..9839
                     /gene="orn"
                     /locus_tag="WM704_001663"
     CDS             9276..9839
                     /gene="orn"
                     /locus_tag="WM704_001663"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFA9341037.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2527 a         2805 c         2503 g         2033 t
ORIGIN      
        1 ggaaagattg ccaccattcg gagaatgccc tccccccagc cctcccccac gggggaggga
       61 gtgggtttcg gcagattttt cggttgccgg tggtttgaaa aataacttaa atttgcaacc
      121 gttgatttca ggtcgtctga aaaaggatgc acgaaatatc aacagcggga atttttcagg
      181 tagcctttat tgtcaagcag atggaacaaa cgccgcgagc gttttttcag acaatctttg
      241 aactcatcgg tagagtgtgt gccgcaaggc gcgcatgcgg tgggtcgggg ttacagggaa
      301 aatgaagaac gcgtgcgtgc gtaccgcaca ccctacatgc gggctacggc ttgctaggat
      361 tgctcgttat acttaatttt gacaaggagt caatgaaatg gcgatagaac taaatggtaa
      421 ctggacaaaa ggatttgctt tagaccatca tatgcaaaaa agtattttgt taggttatga
      481 tgaattaggg catcctcact ttgatagcaa gagaagtgtg attggagaat gggtttatca
      541 attaaaatat cgtaatcaaa cccaaaatgt ttctttgctg gtagactata ttttacaaaa
      601 atttagtggt ttagaatcta taaatttaat tgttcccgcc ccttttacaa cagagcgtat
      661 taatcaacca gttcaattaa ttgccaaaga actaagtgat cgcttgaata ttccatattc
      721 tcctatttta agaaaatcta gctcacacac accactcaaa aatattgaag aaaaatcaga
      781 aaaactagaa atactgaata attccatcac aattgacaat atagatctat ccaataagaa
      841 tattttagta atcgatgatt tatttgatag cggagcaaca ttggaaatat caactgaaaa
      901 attgttttca aaaaatgcta gatctgtaat tgttttagca atgacaaaga ctagaggata
      961 aaaatgcgca ctattttctt ttctggttca cgcagcattt cacgactaaa tccacaaata
     1021 cgagaaagaa ttaacaatat tcttagcaat aattttgata ttgttattgg agatgctaat
     1081 ggggctgata aagctatcca agaatttcta caagaacaag attatgctaa tgtgcatatt
     1141 tacttttctg gcaaaattta ccgaaataat gtgggaaatt ggcagtttgt ccaagtagac
     1201 agcaaaggaa ctggaagggt attttataca gcaaaagata agaaaatggc agaaattgct
     1261 gattatggct ttattttatg ggatggtaag agtattggtt cattaaataa tatagcagaa
     1321 cttttacagc taaataaacc ttctcttgta taccattctc aaacaaaaga attctttaaa
     1381 attaaatcaa gcgcagattt agagaatatt ttatctaaca tagaagatga tgttttagct
     1441 tcaattttag aaaagggaaa tacttttctt aaaagctatg taacaaaaca accttcttta
     1501 attcaagagt aaccacccat gcccaaacgt accgacctaa aatccatcct tatcatcggc
     1561 gccggcccta tcgttatcgg tcaggcctgc gaatttgact attcgggcgc acaggcctgc
     1621 aaagccttgc gtgaagaagg ctataaagtc attttggtga attccaaccc cgccacgatt
     1681 atgaccgacc ccgaaatggc ggatgttacc tacatcgagc cgattatgtg gcagacggtg
     1741 gagaagatta tcgccaagga gcggcccgat gcgattctgc ccacgatggg cggtcagacc
     1801 gcgctgaact gtgcgctgga tttggcgcgt aacggcgtgc tggcgaaata caatgtcgag
     1861 ttaatcggcg caacggaaga cgcgatcgac aaggcggaag accgcggccg ctttaaagaa
     1921 gcgatggaaa aaatcggcct ctcttgcccg aaatcttttg tctgccacac catgaacgaa
     1981 gccttggcgg cgcaagaaca ggtcggcttt ccgacgctga ttcgtccgtc tttcacgatg
     2041 ggcggttcgg gcggcggcat tgcctacaat aaggatgagt ttttggcgat ttgcgaacgc
     2101 ggtttcgatg cgtcgcctac gcatgagctg ctgattgagc agtctgtgct cggctggaaa
     2161 gagtacgaga tggaagtggt gcgcgataag gcggacaact gcatcatcat ctgttcgatt
     2221 gaaaacttcg acccgatggg cgttcatacg ggcgactcga ttacggttgc gccggcgcaa
     2281 acgctgacgg acaaggaata ccaaatcatg cgcaacgctt cgttggcggt attgcgcgaa
     2341 atcggcgtgg acacgggcgg ctcgaacgtg cagtttgcgg tgaaccctga aaacggcgag
     2401 atgattgtga tcgagatgaa cccgcgcgtg agccgttcgt ccgcgctggc ttccaaagca
     2461 acgggcttcc cgattgcgaa ggtggcggcg aagctggcgg tcggctttac gctggacgag
     2521 ttgcgcaacg acatcaccgg cggccgcacg cccgcgtcgt tcgagccttc catcgactat
     2581 gtggtaacca aaatcccgcg tttcgcgttt gaaaaattcc ccgccgcaga cgaccgcctg
     2641 accacgcaga tgaaatctgt gggcgaagta atggcgatgg gccgtaccat tcaggaaagc
     2701 ttccaaaaag ccttgcgcgg tttggaaacc ggcttgtgcg gtttcaatcc gcgcagcgaa
     2761 gacaaagcgg aaatccgccg cgaactggcc aaccccggcc ccgaacgtat gctgtttgtg
     2821 gcagacgcgt tccgcgcggg cttcacgccg gaagaaatcc acgagatctg cgccatcgac
     2881 ccttggttct tggcgcaaat cgaagacttg atgaaggaag aaaaatcggt ttcagacggc
     2941 cagttgcaag atttggatta cgccgcccta cgccgtctga aacgcaaagg cttctccgac
     3001 aaacgtttgg cacaattgtt gaacgtaagc gaaaaagaag ttcgcgaaca ccgctacgcg
     3061 ctgaagctgc atccggttta caaacgcgtc gatacctgcg ccgccgagtt cgccaccgaa
     3121 accgcctatc tttactccac ttacgaagaa gaatgcgaat cccgtccttc cgaccgtaaa
     3181 aaagtgatga ttctcggcgg cggcccgaac cgcatcggtc agggcatcga gtttgactac
     3241 tgctgcgttc acgccgcact cgccctgcgc gaatcgggct ttgaaacgat tatggtcaac
     3301 tgcaaccccg aaaccgtgtc caccgacttc gacaccagcg accgcctcta tttcgagccg
     3361 ctgacattgg aagacgtgtt ggaaatcgtc cgcaccgaaa acccgtgggg cgtgattgtt
     3421 cattacggcg gtcaaacccc gctgaaactc gccaacgcat tggttgaaaa cggcgtgaac
     3481 atcatcggca catccgccga cagcatcgat gccgccgaag accgcgaacg cttccaaaaa
     3541 gtgttgaacg acttaggcct gcgccaaccg cccaaccgca tcgcccacaa cgaagaagaa
     3601 gcactcgtca aagccgaaga aatcggttat ccgctggtcg tgcgcccgtc ttatgttttg
     3661 ggcggacgcg cgatgcagat tgtccactcc gccgaagcct tgcaaaaata catgcgcgaa
     3721 gccgtgcagg tttccgaaga cagccccgtg ctgctcgatt tcttcctcaa caacgcgatt
     3781 gaagtcgatg tggactgcgt ttcagacggc aaagatgtgg ttatcggcgg catcatgcag
     3841 cacgtcgaac aggcgggcat ccactccggc gactccggct gctcgctgcc gccatactcc
     3901 ttaagcgaag aaatccaaga cgaaatccgc cgccaaacca aagcgatggc gtacgcgctg
     3961 ggcgtggtcg gactgatgaa cgtgcagttt gccgtgcagg acggcgtggt gttcgtgttg
     4021 gaagtgaacc cgcgcgccag ccgtaccgtc ccctttgtct ccaaagccac cggcgtgccg
     4081 ctcgccaaag tcggcgcgcg ctgcatggca ggcatttccc tgaaagaaca aggcgtggaa
     4141 aaagaagtcg tccccgattt ctatgccgtt aaagaagccg tgttcccatt catcaaattc
     4201 ccgggcgtgg atacgatttt gagtccggaa atgcgctcca ccggcgaagt gatgggcgtg
     4261 ggcgcaagtt tcggcgaagc ctactacaaa gcccaactcg gcgcgggcga acgcctcaac
     4321 ccgaccggca aaatcttcct ctccgtgcgc gaagaagaca aagaacgcgt cattaaaacc
     4381 gccaaaaact tccaagcctt aggctacggc atctgcgcca cgcgcggcac ggcgcaatac
     4441 ctgaccgaac acgggctgat tgtgcaggcc atcaacaaag tccccgaagg ccgcccgcac
     4501 atcggcgacg cgctgaaaaa cggcgaaatc gcactggtcg taaataccgt ttccagcgat
     4561 ccgcaatccg tgtccgacag ccacatcatc cgccaaagcg cattgcagca acgcgtgccg
     4621 caatacacca ccaccgccgg cggcgaagcg atgagcgaag gcgcgaaaag ccgggattat
     4681 ctgggtgtgt acagcgttca agagctgcac ggtcgtttga aaaaccgcaa ctgatgcctg
     4741 aatcaggttg aacaatgccg tctgaagccg ttttgcggtt tcagacggca ttttgttgca
     4801 catagccttc actttctttt gacctttaag caccctaatt tggggcggtg gattatccaa
     4861 ctgccggaca tccataaaaa taccgcccga accacggttc agacggcatc tgccttgcaa
     4921 cggacagcag cagtttaaag cccgccccct tcgttccgcc ccaaaaccat cgcatctccg
     4981 tagctgaaga aacggtattc acgttcaatc gcatgacggt acacggcgcg gatatgaccc
     5041 atacccgaaa acgcgccgac caacatcagc agcgtcgatt tcggcagatg aaaattggta
     5101 accagcctgt cgacaacatt aaaacggtag cccggcgtga tgaaaatatc ggtgtcgccc
     5161 tgtccgtctt tcaaatatcc cgttgcgcgc gcggcagact cgagggcgcg catggaagtc
     5221 gtgccgaccg cccaggcttt gttcccccgg gcttttgccg cctcaacggc ggcgacggtt
     5281 tcagacggca cttcaaacca ttcgctgtgc attttgtgtt cttcgatttt atcgacgcgc
     5341 acgggttgga atgtccccgc accgacgtgc agggttactt ctgcggttac cgcgcctttg
     5401 tctttcagac ggcgcaaaag ttcttccgta aaatgcaggc ccgccgtcgg cgcggcgacc
     5461 gcgccctgat atttggcata aacagtttga taacggctgt cgtcgtccgc atcggcggca
     5521 cgttcgatat aaggcggcag gggcaggtgt ccgttctgtt ccaaaagttc gtaaacggtt
     5581 tgaccgcctt caaaacgcag gcagaacagt tcgccctcac gcccgaccat cacggcacgg
     5641 ataccgcctt caaacaccag ccccataccg ggcttggggg acttggacga acggatgtgc
     5701 gccaatgcgg tatggttgtc caacacacgc tcaatcaggg cttcgatcct gccgccgctg
     5761 tctttctgcc caaacagccg cgccttcatg actttggtgt tgttgaatac caaaacgtcg
     5821 cctgcctcga cataatcggg caaatcgcca aacacccggt cttgcagcgg catatcgggc
     5881 agcgcgacca aaagccggct gctgccgcgc acctcgggcg gatgctgggc aatcaggtgt
     5941 tcgggcaggg taaagtcaaa atctgaaata tccattttta cactctcgtt cgggcaagcc
     6001 gccattatac gcactttagc cctttttcag acggcatctt tgcccgaaaa accaacagat
     6061 tagaataaac actcttgact cggggcatct tgtgcgcaaa atcaaacttc ctgcacattt
     6121 cccccaaaaa ccgccgtttt ttgatatttt actggacatt taccgacaac ttcgggaaaa
     6181 tgaacacaat ttcacggtcg tttcccacca caggaaaacc gtatccgaac accattccgc
     6241 ccggtttgcg ccgttgccgc aagccggctg ttttctgaaa aaccaacgca acaacccgcc
     6301 ggaacaccgg cagcctttaa aggaacagaa atggatttgc gcaaattaaa aaaactgatt
     6361 gatttggttg aagaatcggg tatcgccgaa atcgaagtaa ccgaaggcga ggaaaaagtc
     6421 cgcattaccc gtaccattgc agctgcggca gctcccgttt acgccgcgcc cgtacctgcc
     6481 gccgcgccgg ccgtaacgcc ggccgccgca cccgttgcgg catccgcgcc cgccgccgcg
     6541 ccggccgccc gcgatttgtc cgacgctcaa aaatcgccta tggtcggcac gttctaccgc
     6601 gcgcccggcc cgaatgccgc cgcatttgtc gaagtcggtc aacaggtcaa agccggcgac
     6661 acgctgtgca tcatcgaagc gatgaagctg atgaacgaaa tcgaagccga aaaatccggc
     6721 acggtcaaag aaattctggt cgaaaacggt acgcccgtcg aattcggcga accgctcttc
     6781 atcatcggat aatcctgttt tcagacggca taaacttccg atgccgtctg aaatgctttc
     6841 ccccccttca gcgttcccgc accctttttt acggacgggt tgccggaacc gcaggaaagg
     6901 tcatcatgct gaaaaaagtt ttaatcgcca accggggcga aatcgccttg cgcgtactcc
     6961 gtgcctgccg cgaaatgggc attgccaccg ttgccgtgca ttccgaagcc gacaaaggca
     7021 gcctgcacgt caaactcgcc gacgaatccg tgtgcatcgg tcctgccgct tctgcgcaaa
     7081 gttatctgaa cattcccgcc atcattgccg ccgccgaagt aaccttcgcg gacgctgtcc
     7141 acccgggcta cggcttcctt gccgaaaacg ccgatttcgc cgaacaggtc gaacagtccg
     7201 gctttacctt catcggcccg aaacccgaca ccatccgcct gatgggcgac aaagtctccg
     7261 ccaaacacgc gatgatagcg gcaggcgtgc cctgcgtccc cggctctgac ggcgcattgc
     7321 ccgacgatga tgccgaaatc ctcaaaatcc ccgacaaagt cggttatccc gtgattatca
     7381 aagcatcggg cggcggcggc gggcgcggta tgcgcgtggt cgagaaaaaa gaagacctcc
     7441 tccagtctgt cgaaatgacc aaggctgaag cgggcgcggc gttcggcaac ccgatggttt
     7501 atatggaacg ctacctgcaa cgcccgcgcc acgtcgaaat ccaagtgctt gccgacgaac
     7561 acggcaacgc cgtctatctt gccgagcgcg actgttcgct gcaacgccgc caccagaaag
     7621 tcatcgagga agcacccgcc ccgtttatcg atgaagaggc acgcaaaaaa atcggcaaag
     7681 cctgtaccga cgcgtgcaaa cgcatcggct accggggcgc gggcacgttt gaatttttat
     7741 acgaagacgg cgaatttttc tttatcgaga tgaacacgcg cgttcaggtc gagcatccgg
     7801 ttaccgaact cattaccggc gtggacatcg tccaagagca actccgcatc gcatccggcc
     7861 tgccgctgca atacaaacag aaagacatca aaatcgaagg ccacgccttc gagtgccgca
     7921 tcaatgccga agacccgtac aacttcatcc ccagcccggg cccgattgaa agctgccacc
     7981 tgcccggcgg cttcggcatc cgcgtggaca gccacatcta ccaaggctac cgcatcccgc
     8041 cgtactacga cagcctgatc ggcaaaatct gcgtacacgg caaaacgcgt gaacaggcaa
     8101 tggcgaaaat gcgcgtcgca ctcgccgagc tggcggtaac cggcatcaaa accaatacgc
     8161 cgttgcaccg cgacctgttc gccgatgcgg gtttccaaga aggcggagtg agcatccatt
     8221 acctggagca ctggctggaa gcgcgcaaaa ccaaacagga caaataagcc gccgccgata
     8281 tgccgtctga agccgccctt ccgcgttcag acggcatttc ccttgccccg ccgtctgaaa
     8341 ccgatttcga tatttccaag aaagcccgtt atgccctacc aacaaatcac cgtcaacgtc
     8401 aacgatgccg tcgccgaacg cctcgccgac gcgctgatgg aacacggtgc actctccgcc
     8461 gccatcgaag atgcctgcgc cggcacgcaa aacgaacagg cgattttcgg cgaacccggt
     8521 atgcccaccg aacaaatctg gcagcagagc aaagtcatcg ccctgttcgg cgaacacgac
     8581 gaagccgccg ccgtcatcga tgccgccgca caagaatgcg ggttaaaaga cttggcatac
     8641 accggcgaaa ccatcgaaga ccaagactgg gtgcgcctca cgcaatcgca attcgacccc
     8701 atccgaattt ccgaccgcct gtggattacc ccctcttggc acgaagcccc cgaaggctgc
     8761 gccgtcaacc tccgcctcga tcccggactc gccttcggca ccggcagcca cccgaccacg
     8821 cgcctctgcc tcaaatggct ggatacgcaa ctcaaaaacg gcgaaagcgt cctcgactac
     8881 ggctgcggtt cgggcatcct gaccatcgcc gccctcaaac tcggtgcagg ttccgccgtc
     8941 ggcgtggata ttgacgaaca ggccgtccgc gccggcaggg acaacgccga acagaacaac
     9001 gtcgatgcac aattcttcct gcccgacagt ctgcctcaag ggcaattcga tgtcgtcgtc
     9061 gccaacatcc tcgccaaccc tttgcgtatg ctcggcgaaa tgcttgcagc ccgcaccaaa
     9121 cagggcggac gcatcgtgtt gtccggtttg ttggatgaac aggccgaaga actcggcggc
     9181 atttacagcc aatggttcga tctcgacccg gcggaaactg acgaaggctg ggcgcgcttg
     9241 agcggcgtaa aacgctgaaa cggaaaggaa acaccgtgca ggacaaaaac aacctctgct
     9301 ggctcgatat ggaaatgacg gggctgaatc ccgaaaccga ccgcattatc gaagtcgcga
     9361 tgattattac cgactcggat ttgaatgtgt tggcgcaatc cgaagtttac gccatccacc
     9421 aaagcgacga tctgctcgac aatatggacg agtggaacac cgccacacac ggcaggacgg
     9481 ggctgacgca gcgcgtgcgc gaatcgtcgc ataccgaagc cgaagtcgaa cagaaactgc
     9541 tggactttat gtcggaatgg atacccggac gcgccacacc gatgtgcggc aactccatcc
     9601 accaagaccg gcgttttatg gtcaaatata tgccgaaact ggaaaactac ttccactacc
     9661 gcaacctcga cgtttccacg ctgaaagaac tcgccaaacg ctggaatccg cccattgcca
     9721 aaagcgtcgt caaacgcggt tcgcacaagg cattggacga cattttggag agcatcgaag
     9781 aaatgcgcca ctaccgcgaa cacttcctga tttccgcccc gaaagccgaa gcgcaataag
     9841 aaacaaacaa tgccgtctga aacgctat
//
LOCUS       DAWTHD010000087         9786 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWTHD010000087 DAWTHD010000000
VERSION     DAWTHD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.087"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(1..345)
                     /locus_tag="WM704_001664"
     CDS             complement(1..345)
                     /locus_tag="WM704_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9341038.1"
                     /translation="MLPENGEWPKDNGAAGVASASTIKGKYVQKVEVAKGVVTAQMAS
                     TGVNKEIKGKRLSLWAKRQDGSVKWFCGQPVKRDNADDPNDAVKDDADANGKISTKHL
                     PSTCRDTSSAGT"
     gene            complement(389..826)
                     /locus_tag="WM704_001665"
     CDS             complement(389..826)
                     /locus_tag="WM704_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9341039.1"
                     /translation="MPVFECGRQRVFIHRPAAPAINQNRTGLHLPQLVFAGHYPNNGK
                     WPANNGNAGVASPADIKGKYVESVTVANGVVTAQMKPSGVNNEIKDKRLSLWGRRENG
                     SVKWFCGQPVKRDAGADDVKADGTKKIETKHLPSTCRDTSSAE"
     gene            719..1027
                     /locus_tag="WM704_001666"
                     /pseudo
     CDS             719..1027
                     /locus_tag="WM704_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
     gene            complement(1102..3156)
                     /gene="metG"
                     /locus_tag="WM704_001667"
     CDS             complement(1102..3156)
                     /gene="metG"
                     /locus_tag="WM704_001667"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFA9341040.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(3272..5110)
                     /gene="glmS"
                     /locus_tag="WM704_001668"
     CDS             complement(3272..5110)
                     /gene="glmS"
                     /locus_tag="WM704_001668"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFA9341041.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5382..5888
                     /locus_tag="WM704_001669"
                     /pseudo
     CDS             5382..5888
                     /locus_tag="WM704_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6192..7517)
                     /locus_tag="WM704_001670"
     CDS             complement(6192..7517)
                     /locus_tag="WM704_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFA9341042.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKVLNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7750..8569
                     /locus_tag="WM704_001671"
                     /pseudo
     CDS             7750..8569
                     /locus_tag="WM704_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
     gene            8566..9732
                     /locus_tag="WM704_001672"
     CDS             8566..9732
                     /locus_tag="WM704_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFA9341043.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFAATRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
BASE COUNT         1986 a         2443 c         3023 g         2334 t
ORIGIN      
        1 tcacgtaccg gcagatgacg tatcgcggca ggttgacggc aggtgcttgg tgctgatttt
       61 gccgttggcg tcggcgtcgt ctttgacggc gtcgttgggg tcgtcggcgt tgtcgcgctt
      121 aaccggctgt ccgcagaacc attttaccga accgtcttga cgcttggccc acagggagag
      181 tcttttgcct ttgatttctt tgtttacgcc ggttgaagcc atttgggcgg tgacgacgcc
      241 ttttgcgact tcaactttct gaacatattt gcctttgatt gttgaagcgg atgccacgcc
      301 ggcagcgccg ttgtctttgg gccattcgcc gttttcaggt aatattcggt gagggctgat
      361 ttatttgaaa tttaaggcct aatttgcctt attcggcaga tgacgtatca cggcaggttg
      421 acggcaggtg cttggtttcg atttttttgg tgccgtcggc tttgacgtcg tcggcgccgg
      481 cgtcgcgctt aaccggctgt ccgcagaacc attttaccga accgttttca cgcctgcccc
      541 acagggatag tcttttgtct ttgatttcat tgtttacgcc gcttggtttc atttgggcgg
      601 taacgacgcc gtttgcgacc gtaacgcttt caacatattt gcctttgatg tcggcggggg
      661 atgccacgcc ggcattaccg ttgtttgcgg gccatttgcc gttattcggg taatgtccgg
      721 cgaaaacgag ttgcggcaga tgaagcccgg tgcggttttg attaattgcg ggcgcggcgg
      781 gccggtggat gaaaacgcgc tgcctgccgc actcaaatac gggcagatcg gcggggcggg
      841 cgcggatgtt ttgacgcaag aaccgccgag gggcggcaat cccttgctga atgcacgatt
      901 acccaatctg attgttacgc cgcataccgc gtgggcgagc cgcgaggctt tggatcggtt
      961 gtttgaaata ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc aaaaccgcgt
     1021 ggtttgaacc tgtcgggatt gcggaaaaaa acgccgtctg aacgcccaag gggttcagac
     1081 ggcgtttttt gagatttctg cctaaccgac tttgtcgccc ggctgagcgc ccgcatccac
     1141 gtcaagcagc ttcagtttgc cgtctgccgt ggcggcggag aggatcatac cttcggatac
     1201 gccgaatttt gccattttgc gtggggcgaa gttggcgact gcgatgacca tgcgaccgtt
     1261 caattcggca ggatttgggt aagacgcggc gatgccggag aagatgatgc gtttttcaaa
     1321 accgaaatcg aggtcgaatt tcaaaagttt ggtgctgcct tcgacggctt cgcagttcaa
     1381 tactttggcg acgcgcatat cgattttcat aaagtcgtcg aagcttgcct gttcggcgac
     1441 tttttcgtat ttaccctctt caacgggcgc ggatgccgtc tgaatgcttt gtttgttggc
     1501 ttcgatcaaa tcgtccactt gtttttgctc cactcgttgc attaaatgtt cgtatttgtt
     1561 gatggcgtgt tcgcctaggg tttcgcgcgt gttcttccag gtaatcgcgt ccagattgag
     1621 gaaacgcgcg gcgttggcgg cggtttgcgg caacacgggg gcgaggtagg cggtcagcat
     1681 ggtgaaggcg ttgatgagtt cgctgcatac ttcgtgcagg cgttcgtctt gaccttcttg
     1741 tttggcgagc tcccacggtt tgttggcatc gacgtattcg ttgacaatgt ctgccaatgc
     1801 catgatgtcg cgcagggcgc gggcgtattc gcggttttcg tattgctcgg cgatggtgtc
     1861 gctttcggcg gcgagttttg ccagcaatgc gctgcctgaa acgtctttcg gacgaccttc
     1921 aaagcgtttg gcgatgaaac ccgaagcgcg ggcggcgatg ttgacgtatt tgccgacaag
     1981 gtcgctgttg acgcgcgaaa taaagtcttg caggttcaaa tcgatgtctt cgattttgct
     2041 gttgagcttg gcggcgatgt agtagcgcat ccactcgggg ttcaggcctt gttccaaata
     2101 ggatttggcg gtgatgaagg tgccgcgcga tttggacatt ttttgtccgt ccacggtcaa
     2161 aaagccgtgt gcgtacacgc cggtcggggc gcggtggccg gagaaatgca gcatggccgg
     2221 ccagaacagg gcgtggaaat agaggatgtc tttgccgatg aagtggtaca tctcggtttg
     2281 gctgccggct ttgaagtatt cgtcaaaatc gatgccgatg cggtcgcaca ggtttttaaa
     2341 cgatgccatg tagccgacgg gcgcgtcgag ccagacgtag aagtatttgc ccggcgcgtc
     2401 ggggatttca aaaccgaaat acggcgcgtc gcgggaaatg tcccagtcgg acagggtggt
     2461 ttctccgcct tcgcccagcc attccttcat tttgttgagg gcttcgggtt gcagatgggg
     2521 cttgccgtcg tgcgggttgt tgccggaagt ccatgctttg aggaagtcgg cgcattcgcc
     2581 cagtttgaag aagaagtgtt cggattcgcg caattcgggt ttcgcgccgg aaacggcgga
     2641 atacgggttg aacagttcgg tcggggaata ggtcgtgccg cagacttcgc agttgtcgcc
     2701 gtattggtct tgggcgtggc atttcgggca ttcgcctttg acgaagcggt cgggcaggaa
     2761 catttgtttt tcggggtcga aaagctgctc gatgacgcgg ctttcgattt tgccgttggc
     2821 tttcagcgcg cggtaaatgt cttgggaaaa ctgtttgttt tcagtggaat gggtgctgta
     2881 ataattgtcg tagccgatga aaaagccggt aaagtcggca aggtgctctt tgcgcacttt
     2941 ggcaatcatg tcttcgggcg cgatgccttg tttttgcgcg gcaagcataa cgggcgtgcc
     3001 gtgggtgtcg tcggcgcagc agtagtagca ttcgtggccg cgcagttttt gaaagcgcac
     3061 ccaaacgtcg gtttggatgt gttcgaccat gtggccgagg tggatgctgc cgttggcata
     3121 gggcagggcg gaggtaacta agattttgcg tgtcatattg tgctttgcaa acaatgggta
     3181 aaggcggatt ataccgcaaa tcaaacgggg aaatgccgtc tgaagcctga aaaatcgggc
     3241 ttcagacggc atttttgcca accggcggga tttattcgac ggttacggat ttcgccaggt
     3301 tgcgcggctt gtccacatcc gtgccgcgcg cgagggcggt gtggtaggcg aggagctgca
     3361 cggggacggt atgcacgacg ggggacagtt cgccgacgtg gcgaggtgcg cggataacgt
     3421 gcacgccttc ggcggcatta aaattgctgt cgaggtcggc aaagacgaaa agttcgccgc
     3481 cgcgcgcgcc gacttcctgc atattggctt tgactttgtc caacaggctg tcgttgggcg
     3541 cgatgacgac gacgggcatg ttttcgtcca ccaaggcaag cggcccgtgt ttcagttcgc
     3601 cggcaggata ggcttcggcg tggatgtagg tgatttcctt tagcttcaac gcaccttcaa
     3661 gggcaatcgg gtaatggatg ccgcgcccca aaaacagcgc gctggttttc ttggcaaact
     3721 gttgcgccca agcggcaatt tgcggttcga ggttcaaagc gtgctgcacg ctgccgggaa
     3781 gctggcggag ttcttcggtg taacgtgctt catcttcgcc ggataccacg ccgcgcactt
     3841 tcgccagcgt taccgccaaa ccgaacagca cgaccagttg cgtggtaaat gctttagtcg
     3901 aggcgacgcc gatttccgca ccggcacggg tataaagcac aaggctgctt tcgcgcggca
     3961 gggcggattc catcacgttg cagacggaga ggctgtggcg gtgtcccaaa gatttggcgt
     4021 atttcaaagc ctccatcgtg tccagcgttt cgccggattg ggaaatggta atgaccagtt
     4081 ggtcggggtc ggcaatcacg ctgcggtacc ggtattcgct tgcgatttcg acgtcggccg
     4141 ggattttggc gatggattcc aaccaatatt tggcggtcag cgcggcgtaa taggacgtgc
     4201 cgcaggcaag gattttgacg ctgcggatgc tttcaaacac gcttttggcg tttttgccga
     4261 agttttcggg gatgaatccg ccgtcgagga aaacttccgc cgtgtccgca atcgcgcgcg
     4321 gctgctcgtg gatttctttt tgcataaagt ggctgtgcgg acccggttcc aaagaggcgg
     4381 gcgagagttc ggataccttg actttgcgtt cggcaggcag gccgttttta tcggtcagcc
     4441 gtttgatgcc gtctgaagcc agcagcgcga tgtcgccgtc ttcgaggtac gccacgcggc
     4501 gcgtaaaggc gatgacggcg gatacgtccg aagcgataaa ggtttcatcg tcgcccaaag
     4561 cgaccaaaag cgggcagccc atacgcgcca caactaattc atcaggcttg tcttgggcaa
     4621 taaccgcgat ggcgtatgcg ccgtggaaac gtttgaccgc ttcctgcacg gcttcaaaca
     4681 atttgccgcc gttttgcgcg tattcgtgat tgatgctgtg tgcgatgact tcggtatccg
     4741 tttgcgattc aaaccggtat cccaaacctt ccaagcgttt gcgttcgctt tcaaagtttt
     4801 cgatgatgcc gttgtggacg acggcaatca taccgccgct gatgtgcggg tgggcgttcg
     4861 gctcggtaac gccgccgtgt gtcgcccagc gcgtatgccc gatgccgatg ccgccgctga
     4921 tgcctttttc gcgtgccgcg tcctccataa gctgcacgcg tccgacgcgg cgcacgcgct
     4981 tgattttgcc gtctgaatac acagcaatgc ctgatgagtc ataaccccgg tattcgaggc
     5041 gtttgaggcc gtcggtcaga aaatcgacga cgttgtgatg ggcgcggatg gcgccgacga
     5101 taccgcacat aaactgttcc ttagtatccg gttgaaaaaa aagatgcgga cggcttccgt
     5161 accgcacctt ccgtttcgga ttataaaccg cctcccgcgc cggaaaacag caaaatgccg
     5221 tctgaaggct tgggcttgct caaaaaaggg agggatttcc ccgcctgacc gggatgggcg
     5281 ttcagacggc attgcctgcc gccggtttat agtttttgca aatcaatatt aacaagctga
     5341 aaaaaaacaa tatacccgcc cggtcttaat gttaacggag tatggaaatg aaacaaatgc
     5401 ttttggccgt cggcgtggcg gcggtgttgg cgggctgcgg caaggatgcc ggcggttacg
     5461 agggttattg gcgcgaaaag tcggacaaaa aagagggcgt gattgccgtc aaaaaagaaa
     5521 aaggcaatta cttccttaat aaaatcaacg tgtttacagg caaggaggag tctttgcttt
     5581 tgtctgaaaa agacggcgcg ctttcgataa acacggggat aggggaaatc ccgatcaaac
     5641 tttccgacga cgggaaagag ctgtatgtcg aacgcaggcg gtatgtgaaa accgatgcgg
     5701 cgatgaagga caaaatcatc gcccaccaga aaaagtgcgg acaaacggca caggcatacc
     5761 tcgacgcgcg aaatgcgttg ccgtcaaacc aaacgtatca gcagcgtcag gcggcgatcg
     5821 agcaattgaa acggcggttt gaagccgagt ttgacgaatt ggaaaaagaa atcaaatgca
     5881 acggcagaac cccggcattg ttgctttggc aggggacaac cgggaggatg ccgccgtccg
     5941 aatcggacgc gtggtttcca taccggtacg ggcgggcggg aatgtccgcc tttttttgtt
     6001 cggatgcgtt tgaatacccg tccgattctg tccgtttgca agggggattt ccgttcgggc
     6061 ggaaatccat tccgtcaaat aagaaaggcg ttttgtccgg ccggccggct tgtgcctgcg
     6121 gtatcgcaaa acttcgcctt aatgcgcccg attggctagg gacgggcttc ggacggcatt
     6181 gttttgccgg ttcacgggcg gtattcgggc ttcatgccgt tgggcaggag ctgccagacg
     6241 tatcccgtgg ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa
     6301 taatccacgc gcaccgcgcc tttgatcgcg ctgcctgtat cctgcgccat aatcaggcgg
     6361 ttgaggactt tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc gcccaaggta
     6421 atgtagtgcc ggtcgattgc gccggcgtat tcccccatca gcggcgtgcc cagtgcgccg
     6481 acggggccct cattgccgct tccggcaagc tcgcggaaaa agatatagct ggggttttgc
     6541 cccaaaactt cggcgaggcg ttgcggattt tgccgcatat aggctttgat gccctgcatg
     6601 gaggtttgcc cgagcttgag gtagcctttg tccgccatat agcgtccgat ggaaacatac
     6661 ggatgctcgt ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga
     6721 cggcccgagc cttgtatgtg catgaaaaaa agttcgacgg ggtcttcggc gtaaccgagt
     6781 atcggggctt tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg
     6841 aagcggcttc cttcaaacct gcccttgatt gccgttgtgc gcgcggtaat ggggaatcgg
     6901 gaaaggtcgg cggtatgcgt gccgccggca ttgtcgattg tgccgctgtt ttttcccgtc
     6961 tgcctgatgc ggacaagggc tttcccgccc cgcaaaccgg caggcagggg gacggagata
     7021 aaatcgtcgg gaatgccgta aatcgggaag cgggcccgtt ccgtccgcct gtcgtcgccc
     7081 ttcagcaccg gctcgtaata gccggtaacc gtacctgcaa ggcttccgtt gcctgcaacc
     7141 tgccacggcg tgaaatagcg ttcaaaaaac cgctttgcct gaaaggaatg cacgggggtt
     7201 tgaaaggctt gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg
     7261 aggcggaagg attgcaggct tttggcaaaa tcctgcgccg cccagtgggg catggacagg
     7321 tgcggcacaa cggtatagac ggccccgccg ccggcaaccg tcgttccggc ggggtcgggg
     7381 atgccggccg gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg
     7441 atgctcctgc tttggcaggc ggcgaggatg gcggcggcga tgccgtacag ggcggagcgg
     7501 agcaggtgtt ttttcataat ggacaatgtt gtcgtcagta ataaaaaaag atggtttcgg
     7561 gcggcgttgc ggtagccggg gagaggggat tttaacacag ggcgcagccg cagcctgcgg
     7621 aactttccgc cacgcggcaa tgcagataaa aataacttgc atttgtattt gcaagtaatg
     7681 aaaatattcc gataatatat tattaattat ccttgttcat tcgcgtttac gctggtcgct
     7741 tttttaatta tgttgcgcga gggtatcgaa gccgcgctca tcgtcggcat cgttgccggt
     7801 tttttgaaac agtccggaca ttcaaaaact gatgcctaag gtctggttcg gggtcgtcct
     7861 tgcttctttg atgtgtttgg gcatcggata cggcatccat tcggcaacgg gcgagattcc
     7921 ccggaagcag caggagttcg tcgtcggcat tatcggtttg gttgccgtcg cgatgctgac
     7981 ttgtgtggtt ttatggatga aaaaggcggc gcgttcgatg aagcggcagc ttcgggattc
     8041 tgtacaggcg gctttgaacc gtggcagcgg tcaaggatgg gccttggtcg gtatggcgtt
     8101 tcttgccgtg gcgcgcgaag gtttggagag cgtttttttc ctgcttgccg tattcaaaca
     8161 gagcccgacg tggcagatgc cggccggcgc ggtagcgggg gttttggctg ccgccgtgat
     8221 tggcgcgttg atttatcagg gcgggatgcg cctgaatctg gcgaagtttt tccgttggac
     8281 gggggcgttt ctgattatcg ttgccgccgg tctgcttgcc ggctcgctgc gtgcgctgca
     8341 tgaggcaggc atttggaacg cgcttcagga cattgtgttg gacccatcaa aatatttgca
     8401 cgaagacagt ccgttgggtg tgctgctcgg cggatttttc ggctataccg accatccggc
     8461 gcagggcggg gccttggttt ggctgctgta ccttattccc gtcatgattt ggtttttgcg
     8521 cggcagcagg ccgtctgaaa ctttaacccg taaagaggaa ttgaaatgag aaaattcaat
     8581 ttgaccgcat tgtccgtgat gcttgccttg ggtttgaccg cgtgccagcc gccggaggcg
     8641 gagaaagccg cgccggccgc gtccggtgag acccaatccg ccaacgaagg cggttcggtc
     8701 ggtatcgccg tcaacgacaa tgcctgcgaa ccgatgaatc tgaccgtgcc gagcggacag
     8761 gttgtgttca atattaaaaa caacagcggc cgcaagctcg aatgggaaat cctgaagggc
     8821 gtgatggtgg tggacgaacg cgaaaatatc gccccggggc tttccgacaa aatgaccgta
     8881 accctgctgc cgggcgaata cgaaatgacc tgcggccttt tgaccaatcc gcgcggcaag
     8941 ctggtggtag ccgacagcgg ctttaaagac accgccaacg aagcggattt ggaaaaactg
     9001 ccccaaccgc tcgccgacta taaagcctac gttcaaggcg aggttaaaga gctggcggcg
     9061 aaaaccaaaa cctttaccga agccgtcaaa gcaggcgaca ttgaaaaggc gaaatccctg
     9121 tttgccgcca cccgcgtcca ttacgaacgc atcgaaccga ttgccgagct tttcagcgaa
     9181 ctcgaccccg tcatcgatgc gcgtgaagac gacttcaaag acggcgcgaa agatgccgga
     9241 tttaccggct tccaccgtat cgaacacgcc ctttgggtgg aaaaagacgt atccggcgtg
     9301 aaggaaaccg cggccaaact gatgaccgat gtcgaagccc tgcaaaaaga aatcgacgca
     9361 ttggcgttcc ctccgggcaa agtggtcggc ggcgcgtccg aactgattga agaagcggcg
     9421 ggcagtaaaa tcagcggcga agaagaccgt tacagccaca ccgatttgag cgacttccaa
     9481 gctaatgcgg acggatctaa aaaaatcgtc gatttgttcc gtccgttgat tgaggccaaa
     9541 aacaaagcct tgttggaaaa aaccgatacc aacttcaaac aggtcaacga aattctggcg
     9601 aaataccgca ccaaagacgg ttttgaaacc tacgacaagc tgagcgaagc cgaccgcaaa
     9661 gcattacagg ctcctattaa cgcgcttgcc gaagaccttg cccaacttcg cggcatactc
     9721 ggcttgaaat aagccgcaag cgttcagacg gcatttggcg gcagataccg tctgaagttt
     9781 tatagc
//
LOCUS       DAWTHD010000088         9480 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWTHD010000088 DAWTHD010000000
VERSION     DAWTHD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.088"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(120..1262)
                     /locus_tag="WM704_001673"
     CDS             complement(120..1262)
                     /locus_tag="WM704_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9341044.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLEAADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1567..1830
                     /gene="rpsT"
                     /locus_tag="WM704_001674"
     CDS             1567..1830
                     /gene="rpsT"
                     /locus_tag="WM704_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFA9341045.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1990..3138
                     /locus_tag="WM704_001675"
     CDS             1990..3138
                     /locus_tag="WM704_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFA9341046.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3280..3981
                     /locus_tag="WM704_001676"
     CDS             3280..3981
                     /locus_tag="WM704_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFA9341047.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4039..5847
                     /gene="aspS"
                     /locus_tag="WM704_001677"
     CDS             4039..5847
                     /gene="aspS"
                     /locus_tag="WM704_001677"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFA9341048.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVATGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5997..6281
                     /locus_tag="WM704_001678"
     CDS             5997..6281
                     /locus_tag="WM704_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9341049.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6448..8340
                     /gene="speA"
                     /locus_tag="WM704_001679"
     CDS             6448..8340
                     /gene="speA"
                     /locus_tag="WM704_001679"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFA9341050.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8439..9362
                     /gene="speB"
                     /locus_tag="WM704_001680"
     CDS             8439..9362
                     /gene="speB"
                     /locus_tag="WM704_001680"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFA9341051.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
BASE COUNT         2327 a         2436 c         2457 g         2260 t
ORIGIN      
        1 ctataaatgt ccgcaatctg aaatcccggc cgtgggggcg acggtataaa tatttctatt
       61 tccatccaat ataaaatgcc gtccgaacat cgttcggacg gcatttttaa tgcttcgaat
      121 cagttggtgc cgactttgat tttctgccac aggctgaccg acagtttttt cgcatccgtg
      181 ctcatttgcg gcatgacgaa accgtctttc atatcctgct cgttcgggaa gatggaacgg
      241 gtgttcacca actcggcagg cattttttcg cgcgccggtt tgctggcggg ggcgaaggtt
      301 acggcgatgc cgtttttcgc cgcgatttcg gggtcgagcg tgtagttgat gtatttgtgg
      361 gcgttggcga cgtttttcgc atcggcggga atcagccaag actcaatcca gaagcccata
      421 cctttcggtg tcagcacttc gatgccgacg ttgtttttca cttcttcgga acgcgctttc
      481 gccaagttca aatcgccgcc gttgcctgcc gccaggcaga tgtcgccgcg cgccagctcg
      541 tcgatgatgg acgggctgaa acgtttgacg tccggacgga tagacttcaa cacttccgcc
      601 gccgccttca aatcttcagg attcgaacct ttggggtctt tgcccaagta gttcagcaaa
      661 atcgggaaca tttcgctcgg ggtgtcccac aaggcgatgc cgcaggattt cagcttgcgg
      721 gtgtattcgg gtttgaacag caaatcccag ccgttttcgg gcagcttgcc gcccaaaagc
      781 tctttgccct tcgccgtaat cgcaaccgtg ttcacgccgg agaaataggg gacggcatac
      841 tggttgcccg ggtcggcggc ttccagcatt ttcaagagtt cgggatcgat gtttttatag
      901 ttgggaatca ggtctttgtt gactttttga tacgcgcccg cctcgatttg gcgcggcagg
      961 aaggcgatgc ccggcacaac caaatcgtaa ccggatttgc cggtcagcat tttggcttcc
     1021 agcgtttcgt tgttttcgta caagtcgtaa gtcagcttca gattgttggc ttttttaaag
     1081 tcttcgaccg tactctcgtc aacatagttc gaccagttgt agatgttcag agtatcggtg
     1141 gcagcggctt cggcattggc agcaggtgcg ctgcctgctt gaggctgcac ggcgtttttt
     1201 tcgctgccgc cgcaggctgc cagagacagc gcggccaaaa cggctaatac ggattttttc
     1261 atacgggcag attcctgatg aaagaggttg gaaaaaaaga aaaccccgcg ccccatagac
     1321 accccggcgc aaggtttggg cattgtaaag taaatttgtg caaactcaaa gcgatattgg
     1381 cctgattttc ctaaaaaatt accctgtttc caaaaaaggg gggggaaacg gccgcccgat
     1441 tttgccgttt ttttgcgccg tcagggtgtc cgacgggcgg atagagaaaa aaggcttgca
     1501 tataatgtaa acccccttta aaattgcgcg tttacagaat ttatttttct ttcaggagat
     1561 tccaatatgg caaacagcgc acaagcacgc aaacgtgccc gccagtccgt caaacaacgc
     1621 gcccacaacg ctagcctgcg taccgcattc cgcaccgcag tgaaaaaagt attgaaagca
     1681 gtcgaagcag gcgataaagc tgccgcacaa gcggtttacc aagagtccgt caaagtcatc
     1741 gaccgcatcg ccgacaaagg cgtgtttcat aaaaacaaag cggctcgcca caaaagccgt
     1801 ctgtctgcaa aagtaaaagc actggcttga tttttgcaaa accgccaagg cggttgatac
     1861 gcgataagcg gaaaaccctg aagcccgacg gtttcggggt tttctgtatt tcgggggtaa
     1921 agttcgaaat ggcggaaagg gtgcggtttt ttatccgaat ccgctataaa atgccgtttg
     1981 aaaaccaata tgccgacaat gggggcggag atgaatacac gaaatatgcg ctatattctt
     2041 ttgacaggac tgttgccgac ggcatccgct tttggagaga ccgcgctgca atgcgccgct
     2101 ttgacggaca atgttacgcg tttggcgtgt tacgacagga tttttgcggc acagcttccg
     2161 tcttcggcag ggcaggaagg gcaggagtcg aaagccgtac tcaatctgac ggaaaccgtc
     2221 cgcagcagct tggataaggg cgaggcggtc attgttgttg aaaaaggcgg ggatgcgctt
     2281 cctgccgaca gtgcgggcga aaccgccgat atctatacgc ctttgagcct gatgtacgac
     2341 ttggacaaaa acgatttgcg cgggctgttg ggcgtacgcg aacacaatcc gatgtacctt
     2401 atgccgtttt ggtataacaa ttcgcccaac tatgccccga gttcgccgac gcgcggtacg
     2461 actgtacagg aaaaattcgg acagcagaaa cgtgcggaaa ccaaattgca ggtttcgttc
     2521 aaaagcaaaa ttgccgaaaa tttgtttaaa acccgcgcgg atctgtggtt cggctacacc
     2581 caaagatccg attggcagat ttacaaccaa ggcaggaaat ccgcgccgtt ccgcaatacg
     2641 gattacaaac ctgaaatttt cctgacccag cctgtgaagg cggatttgcc gttcggcggc
     2701 aggctgcgta tgctcggtgc gggttttgtc caccagtcca acggacagag ccgtcccgaa
     2761 tcgcgttcgt ggaacaggat ttatgccatg gcaggcatgg aatggggcaa attgacggtg
     2821 attccgcgcg tgtgggtgcg tgcgttcgat cagagcggcg ataaaaacga caatcccgat
     2881 attgccgact atatggggta tggcgacgtg aagctgcagt accgcctgaa cgacaggcag
     2941 aatgtgtatt ccgtattgcg ctacaacccc aaaacgggct acggcgcgat tgaagccgcc
     3001 tacacgtttc cgattaaggg caaactcaaa ggcgtggtac gcggattcca cggttacggc
     3061 gagagcctga tcgactacaa ccacaagcag aacggtatcg gtatcgggtt gatgttcaac
     3121 gactgggacg gcatctgaat accgtttcaa acggcatatc aagttgccgt gccgtctgaa
     3181 gccgccggcg gtttggcggg gcgggataaa aaattcgttt gaatccgggc aaacgcgtat
     3241 aatgtgcggt ttgtacggac tttgtcggaa tcaagcaaga tgacggaacc tgcggccgaa
     3301 ggcggcaaag ctgccaaggc gttaaaaaaa tatctgatta caggcatttt ggtctggctg
     3361 ccgattgcgg taacggtttg ggtggtttcc tatatcgttt ccgcgtccga ccagcttgtc
     3421 aacctgctgc cgaagcaatg gcggccgcaa tatgttttgg ggtttaatat ccccgggctc
     3481 ggcgttattg ttgccattgc cgtattgttt gtaaccgggt tatttgccgc aaacgtgttg
     3541 ggccggcaga ttcttgccgc gtgggacagc ctgttgggtc ggattccggt tgtcaaatcc
     3601 atctattcga gtgtgaaaaa agtatccgaa tcgctgctgt ccgacagcag ccgttcgttt
     3661 aaaacgccgg tactcgtgcc gtttccccaa tcgggtattt ggacaatcgc attcgtgtcc
     3721 ggtcaggtgt cgaatgcggt taaggccgca ttgccgcagg atggcgatta tctttccgtg
     3781 tatgtcccga ccacgcccaa cccgaccggc ggttactata ttatggtaaa gaaaagcgat
     3841 gtgcgcgaac tcgatatgag cgtggacgaa gcgttgaaat atgtgatttc gctgggtatg
     3901 gtcatccctg acgacctgcc cgtcaaaaca ttggcaggac ctatgccgcc tgaaaaggcg
     3961 gagttgcccg aacaacaata aagccgccgt tcagacggca ttttccgttt tcagtttaaa
     4021 tcaataaaag gtgattttat gcgtaccaac tattgcggcc tgatcagcga gcaatactta
     4081 ggccaaaccg ttaccgtcaa aggctgggta caccgccggc gcgaccacgg cggtgtgatt
     4141 tttatcgacc tgcgcgaccg cgaaggcatc gtccaagtcg tgatcgatcc cgacacgccc
     4201 gaagcgtttg ccgctgccga ttccgcccgt aacgaatacg ttttgggcat taccggccgc
     4261 gtacgcaacc gtcccgaagg cacgaccaac gataaaatga tttccggcaa aatcgagatt
     4321 cttgccaaag aaatcgaagt gttgaacgcc gccgctacgc cgccgttcca aatcgacgac
     4381 gaaaacatca gcgaaaacgt gcgcctgacc aaccgcgtta tcgacctgcg ccgccctgtg
     4441 atgcagcgca acctgcgcct gcgttaccaa gtcgctacgg gcgttcgccg ttatctggac
     4501 gcgcaaggct tcatcgacat cgaaacgccg atgctgaccc gctccactcc ggaaggcgcg
     4561 cgcgactacc tcgtaccgag ccgcgttcat ccgggcgagt ttttcgcgct gccgcaatcg
     4621 ccgcaattat tcaaacaact gttgatggtg gcgggtttcg accgttacta ccaaatcacc
     4681 aagtgcttcc gtgacgaaga cttgcgcgcc gaccgccaac ctgaattcac tcaaatcgac
     4741 ttggaaacct cgttcctgaa cgaggatgaa atcatggata tcactgaagg catggccaaa
     4801 caagtcttca aagatgcttt gaacgtagat ttgggcgact tcccacgcat gccttattct
     4861 gaagccatgt tctactacgg ctctgacaaa ccggacatgc gcatcaactt aaaattcacc
     4921 gagttgaccg acctgatgaa aacggaagaa ttcaaagtct tccgtggcgc agccgacatg
     4981 aaaggcggcc gcgtagtcgc tctgcgcgtg cctaacggcg ccaaattcag ccgcaaagaa
     5041 atcgacgaat acactaaatt tgtcggcatc tacggcgcga aaggtctggc atacatcaaa
     5101 gtaaacgatg cgggcaacct ctccaacggc gaagacagcg gcctgcaatc tccaatcgta
     5161 aaattcttgt ctgaaaacgc cctgaaagaa atcatcgagc gtaccgccgc gcaaaacggc
     5221 gacatcatct tcttcggtgc ggacaaagcc aaagtcgtga acgaagccgt cggcgcattg
     5281 cgtatcaaag tcggcttgga acacggcaaa gacaacggct acttcacaga cgaatggaaa
     5341 cctttgtggg ttgtcgattt cccgatgttc gaatacgacg aagaagccga ccgctacgtt
     5401 gccgtacacc atccgtttac cgctcctaaa gaaggtcatg aagacctgat ggtttccgat
     5461 cctgcaaact gcctggcccg tgcctacgat atggtattga acggctggga aatcggcgga
     5521 ggctctatcc gtatccaccg tgcagacgta caagagaaag tgtttgccgc gctgaaaatc
     5581 agccctgaag agcaacaaga gaaattcggc ttcctcttgg acaacctgaa attcggtgcg
     5641 cctcctcacg gcggccttgc attcggcctc gaccgtctgg taacgctgat gaccggtgcc
     5701 gaatccatcc gcgacgtgat tgccttcccg aaaacacaac gcgcccaatg cctgctgacc
     5761 gatgcgccca acagcgtgga cgataagcag ttgcgtgaat tgagcctgcg tctgcgtcaa
     5821 aaagcagttg aaacaaaaga agcataagga aaacagagcc gtttgacggc tctgtttttt
     5881 cagacggcat ttacgcttct tgacttccct ctaattcaaa cctaattttg ctgtgtttta
     5941 atggcggtat tgaaaaacac atcttgttca atcaaccgat aaaaaaagga ctgaaaatga
     6001 aaaaactgtt attggccgcc gttgtttccc taaatgccgc aaccgcattt gccggcgact
     6061 ctgccgagcg tcagatttac ggcgatcccc attttgaaca aaaccgcaca aaagccgtga
     6121 aaatgttgga acagcgcggt tatcaggttt acgatgtcga tgccgacgac tactggggca
     6181 aacctgtttt ggaagtggaa gcctataaag acggccgcga atacgacatc gtgttgtctt
     6241 accccgacct gaaaatcatc aaagagcagc tcgatcgctg actcctttga tggaaagatg
     6301 aaccaaaatg ccgtctgaag cgttcagacg gcattttgcc tgttcctcat caggtatgag
     6361 gcaggctttt cttattaaaa aaatgacatt tcacgctgat ttgttataat cattcctttt
     6421 caacacgaca gacggagcag gtttattatg cctatcctta ccatccgtga agtgtgcaac
     6481 attaatcatt ggagcatagg ttattatgat gttgacgatt ccggcgaaat catcgtccgc
     6541 cccaatccct cgcaacacaa tcaaactgtt tcactgcaaa aactgactga agccgtgcaa
     6601 caaaaacatc aggcgcgcct gcctgttttg ttttgttttc cgcaaatcct cgaacaccgc
     6661 ctccgcgaca ttaaccgcgc ctttcagacg gcacgggaag agtgcggcta taagggcggt
     6721 tattgtttgg tttaccctat caaggtcaac caacaccgcc gcgtcatcga gtcgcttatg
     6781 tcaagcggac aaccgcatgg tttggaagcc ggttctaaag ccgaactgat ggcggttttg
     6841 gcacacgccg gcacccggca aacattaatc gtctgcaacg gctataaaga ccgtgaatat
     6901 atccgtttcg ccttgatggg cgaaaaactg gggcatcagg tttatttggt gattgagaag
     6961 ctgtccgaaa tacaaatggt attggaagag gcggaaaaac tcggcatcaa gccccgtttg
     7021 ggtgtgcgcg ccagactggc ttcccaaggt tcgggaaaat ggcagtcttc gggtggggaa
     7081 aaatcaaaat tcggcttgtc ggcttcccaa gttttgcaac tggtcgatat tttgaaacaa
     7141 aaaaacaggc tggactgcct gcagcttttg catttccatt tgggctcgca gcttgggaac
     7201 atccgtgatg ttgccacagg tgtacacgaa tcggctcggt tttatgttga gttgcacaaa
     7261 ctgggggtaa atatccgctg ttttgatgta ggtggcgggc ttggcgtgga ttacgaagga
     7321 aaccgcacac aatcggattg ttccgttaat tacagcctga acgaatatgc cgccacagtc
     7381 gtatggggca tcagtcaggc ttgtctcgaa cacgggctgc cgcatccgac aatcattacc
     7441 gagagcgggc gcggcattac cgcacatcac gccgttttgg ttgctaatgt tataggcgtt
     7501 gaacgttaca aaccgcgtcg gctggatgcg ccatcgcccg aagcaccgcg tgtgttgcac
     7561 agtatgtggg aaacttggac ggatatttcc gcctcgcggg aaaaacgttc cttacgcagc
     7621 tggatacacg aagggcagtt tgatcttgcc gatgtgcata atcagtataa tgtcgggctg
     7681 ttgagtttgg cgcaacgtgc gtgggcggag caactgtatt taaatatctg tcatgaagtc
     7741 ggcgaattgt ttaatgaaaa acaccggtct caccgaacca ttattgacga attgcaagaa
     7801 cgttttgccg ataagctgta tgtcaatttc tcactcttcc aatctttgcc cgatgcttgg
     7861 ggcatagatc aacttttccc tgtttgtcct attaccggtt tgaatgaacc gattgcgcgc
     7921 cgcgccgtgt tgttggacat tacctgcgat tcagacggta cgattgacca ctacatcgac
     7981 ggcgacggca tcgccggtac gatgcctatg cctgattatc ccgaagaaga gccgccgttt
     8041 ttaggctttt ttatggtggg agcatatcag gaaatactcg gcaatatgca caatcttttc
     8101 ggcgacactg ccactgctga tgttgttgta agggaagacg gacaatttac cgtcatcgat
     8161 tacgatgaag gaaacaccgt tgccgatatg ctcgaatacg tttatcaaga tccgaaagag
     8221 ctgatgaaac gctatcgcga acaaatcgaa cattcagacc ttcctgcctc gcaggctatg
     8281 tctttcttaa aagaactcga agcggggctt aatggttata cctatttgga agacgaatag
     8341 acgcatcaag gtatcggata tgtcgtctga agcccgattt tcttactcaa acaccaatca
     8401 tcacgaccga ttgaaaccaa ttacaaggaa tcattacgat gcaatacagc acactggcag
     8461 gacaaaccga caactccctc gtttccaata atttcgggtt tttgcgcctg ccgcttaatt
     8521 ttatgccgta tgaaagccat gccgattggg ttattaccgg cgtgccttat gatatggcgg
     8581 tttcagggcg ttccggcgcg cgtttcggtc ctgaagccat ccggcgcgcc tccgtcaacc
     8641 tcgcttggga gcaccgcagg tttccgtgga catttgatgt gcgcgaacgc ctgaacatta
     8701 ttgattgcgg cgacttggtt ttttcttttg gcgacagcag ggattttgtc gaaaaaatgg
     8761 aagcgcacgc cggcaaatta ctttctttcg gcaaacgctg tttgagtttg ggcggcgacc
     8821 atttcattac cctcccgttg ttgcgcgccc acgcccgcta tttcggcaaa ctcgcactga
     8881 ttcattttga cgcgcacacc gacacctacg acaacggcag cgaatacgac cacggcacga
     8941 tgttttatac cgcccccaag gaaggcctca tcgacccgtc ccgttccgta caaatcggca
     9001 tacgcaccga acacagtaaa aaattgcctt ttactgtgtt gtccgccccc aaagtcaatg
     9061 aagacagtgt tgaagagacc gtccgtaaaa tcaaagaaac cgtcggcaat atgcccgttt
     9121 acctgacttt cgacatagac tgtctcgacc cgtcgttcgc ccccgggacc ggtacgcccg
     9181 tatgcggcgg cttgagcagc gacagggcat taaaaatcct acgtgggctg acggatctcg
     9241 acatcgtcgg tatggatgtt gtagaagttg ccccctctta cgaccaatcc gacattaccg
     9301 ctttggccgg cgccacaatt gccttggaaa tgctttacct tcaaggtgcg aaaaaggact
     9361 gaacgtccgg catcccccgg gttttcgccg tgccgttcaa acggcgtatt cagtctaatg
     9421 aaaattcaaa tactgaaaca aaagttgccc ggagccgcat atcggaaaga cggtgaaata
//
LOCUS       DAWTHD010000089         9126 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWTHD010000089 DAWTHD010000000
VERSION     DAWTHD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.089"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            156..596
                     /gene="nadR"
                     /locus_tag="WM704_001681"
     CDS             156..596
                     /gene="nadR"
                     /locus_tag="WM704_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFA9341052.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            711..1211
                     /gene="hpaC"
                     /locus_tag="WM704_001682"
     CDS             711..1211
                     /gene="hpaC"
                     /locus_tag="WM704_001682"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFA9341053.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            1227..1922
                     /locus_tag="WM704_001683"
     CDS             1227..1922
                     /locus_tag="WM704_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HFA9341054.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(1976..2386)
                     /locus_tag="WM704_001684"
     CDS             complement(1976..2386)
                     /locus_tag="WM704_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFA9341055.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFAAFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNRTFPVLSGLATGASWLAYFKALQLGKAPQVAPVD
                     KFSLVLVALMAVIFLDERPNTQEWIDLGLVTAAC"
     gene            2561..4237
                     /locus_tag="WM704_001685"
     CDS             2561..4237
                     /locus_tag="WM704_001685"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFA9341056.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            4351..5442
                     /gene="ychF"
                     /locus_tag="WM704_001686"
     CDS             4351..5442
                     /gene="ychF"
                     /locus_tag="WM704_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFA9341057.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            5716..7590
                     /locus_tag="WM704_001687"
     CDS             5716..7590
                     /locus_tag="WM704_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFA9341058.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLVGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYALPMLVLGAGSFFAMRLPFMAQYDRLGLTRSNTSCHN
                     NTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYA
                     SRVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRML
                     HKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGCRQTLRPDDESTLKANARIRELA
                     AKYPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH
                     "
     gene            7652..8947
                     /gene="tyrS"
                     /locus_tag="WM704_001688"
     CDS             7652..8947
                     /gene="tyrS"
                     /locus_tag="WM704_001688"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFA9341059.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2108 a         2590 c         2420 g         2008 t
ORIGIN      
        1 aattaaagtc ttttataccc ggtcgtccga tgaggtttat agcgtattgt tgctatatgt
       61 tcgttttgtt atataacggt tgcatcaaaa attacgccca caggctttcc cgacggtttg
      121 aaagtttgat tttcgataac ttggagactt aaacaatgcc tacccaatca aaacatgcgt
      181 ctatcaatat cggtctgata caggcaaggg aagccctgat gacccaattc aggcctattc
      241 tgaatcaggc gaatattacc gatcagcaat ggcggattat ccgtcttttg gcggaaaacg
      301 gcacattgga ttttcaggat ttggcgaatc aggcgtgcat tttgcgcccc agcctgaccg
      361 gtatcctgac ccgccttgaa aaagcggggt tggtcgtccg cctgaaacct tccaacgacc
      421 aacggcgcgt ttatctgaag ctgacttccg agggcgagaa gctgtatgag gaaatcggcg
      481 aagaagtgga cgagcgttac gacgctatcg aggaagtgct gggccgcgag aaaatgctgc
      541 tgcttaaaga cctgttggca gaacttgcca aaatcgagga tgcgttgaac tcgtaatacg
      601 ccgtaatgcg cggaaacgtc cgaccgacgg ctttttgaat cagaactgct gcacatgggg
      661 gatgccttgt gtgcagcatt cttatatagg ggacggttta aaggggaaaa atggcggatt
      721 tgcagaaaac ttttcaaact tcgttccgtg atgcgatggc atcctgtgcg gcaggcgttc
      781 atgtcatcac gacagacggt gcggcagggc gttacggcat tacaatgacg gcggttgcgc
      841 cggttaccga cgagccgccg accgtgatgc tgtgcataaa ccggagtgcg cgaatcattc
      901 cgatcctgtc ggaaaacggc agcctctgca tcaatatgct ggcggacgaa catcaggatg
      961 ttgccgaaca ttttgccggg ctgaccggcc tgtcgcccga agagcggttt gcctaccata
     1021 tttggcatcg cggcaaaacg ggacaacttg aaatagaagg cgcgttggcg cacctgcacg
     1081 ggcatattgt cggcaaacat gaaatcggca cgcattttgt gttttacgtc aggctcgacg
     1141 aaatcaaaaa ctgcgggtgc aaacgccccg cgctgctgta tttcagacgg cagtttaggc
     1201 ctttagactg atattcggac agatatatga aagcgatgat actggcggca ggacgcggcg
     1261 agcgtatgcg ccctttgacc gacaccactc cgaagccgct gctcgatgtg gcgggtaagc
     1321 ctctaatcgg ttggcacttg tgccgtctga agcaggcggg gtttaccgaa atcgtcatca
     1381 accacgcttg gctgggtcgg cagatagaaa atgctttggg cgacggctcg gcttatggcg
     1441 tgaacatcgc ctattcgccc gaacccgcag gcggtttgga aacggcaggc ggcatcgcgc
     1501 aggcattgcc gctgttgggt gggcagccgt ttttggtggc caacggcgac gtgctgaccg
     1561 acatcgattt taccgccgcg tttcagacgg catcgtccct gcccgggcac atttccgcac
     1621 atttgtggct ggtgggaaat cccccgcaca accccgacgg cgatttttcc ctgctgcccg
     1681 acggcagcgt gcggccggaa gtatccggcg gcaacggact gacattcagc ggcgtgggta
     1741 tttaccgtcc tgaaatgttt gacggaatcg aagcgggcag tgtggcaaaa ctcgcgcccg
     1801 tattgctgaa cgaaatgcgg caaaaccgcg tgagcggtca gaagcatacg ggcctgtggc
     1861 tggatgtcgg cacggtatgc cgtctgaagg aagcgcaagc ccttgcagcg gcttggaagt
     1921 gaaaactcgg tttcaggcgg tatggcggat tcggtttaac gtttcagtgc caacgtcaac
     1981 acgccgccgt aaccagcccc aagtctatcc attcctgcgt gttcgggcgt tcgtctaaga
     2041 aaatcaccgc catcagcgcg accaagacca ggctgaattt gtcgacgggc gcgacttgcg
     2101 gggctttgcc cagttgcagg gctttgaagt aggcgagcca agatgcgccg gtggcgagtc
     2161 cggacaaaac cggaaacgtc cggttgcgcc ccgtgaagcc gtttacgccc tgccatttgc
     2221 cggtgtaggt caaaaacaat accaaggcgg cgaggatgac caaggtgcgg ataaaggcgg
     2281 cgaaatccga atctatgccc tgcaagccca ttttggcgaa aacggcggtc aatgaggcga
     2341 agcctgccga tgccaatgcc caaaacagcc atgcgttgct gcccatgttt tctcctttga
     2401 ttgtgaacaa tatgaacggt atttttgttg ctgcgtcaaa aatttcactg cgggtttggt
     2461 gcggataacg ttataatatg cctgatatta ttttcaatcc acctgtttgt cgcctgatgc
     2521 tttcagacgg catgtccctc ctcatttcta aaggaaaatc atgagcttca aaaccgatgc
     2581 cgaaaccgcc caatcctcca ccatgcgccc gattggcgaa attgccgcca agctgggttt
     2641 gaacgttgac aacattgagc cttacggtca ttacaaagcc aaaatcaatc ctgccgaagc
     2701 gttcaagctg ccgcaaaaac aaggcaggct gattttggtt accgccatca acccgactcc
     2761 ggcgggcgaa ggcaaaacca ccgtaaccat cggtttggcg gacgcattgc gccatatcgg
     2821 caaagactct gtgattgctt tgcgcgagcc ttctttgggt ccggtgttcg gcgtgaaagg
     2881 cggcgcggca ggcggcggct acgcgcaagt tttgccgatg gaagacatca acctgcactt
     2941 caccggcgac ttccacgcca tcggtgcggc gaataacctc ctcgccgcca tgctcgacaa
     3001 ccatatctac caaggtaacg agttgaacat cgaccccaaa cgcgtgctgt ggcggcgcgt
     3061 ggtcgatatg aacgaccgcc agttgcgcaa catcatcgac ggtatgggca agcctgttga
     3121 cggcgtgatg cgtcccgacg gcttcgacat caccgtcgcc tccgaagtga tggcggtatt
     3181 ctgccttgcc aaagacatca gcgatttgaa agagcgtttt ggcaatattc tcgtcgccta
     3241 cgccaaagac ggcagccccg tttacgccaa agatttgaag gcacacggcg cgatggcggc
     3301 attgctaaaa gatgcgatta agcccaattt ggtgcaaacc atcgaaggca ctccggcctt
     3361 tgtacacggc ggcccgttcg ccaacatcgc ccacggctgc aactccgtta ccgcaacccg
     3421 tctggcgaaa caccttgccg attacgccgt aaccgaagca ggcttcggcg cggacttggg
     3481 tgcggaaaaa ttctgcgaca tcaaatgccg ccttgccggt ttgaaacctg atgcggcagt
     3541 cgtcgtggcg actgtccgcg ccctgaaata caacggcggc gtggaacgcg ccaaccttgg
     3601 tgaagaaaac ctcgaagctt tggcaaaagg tttgcccaac ctgttgaaac acatttccaa
     3661 cctgaaaaac gtattcggac tgcccgtcgt cgttgcgctc aaccgcttcg tgtccgactc
     3721 cgatgccgag ttggcgatga ttgaaaaagc ctgtgccgaa cacggcgttg aagtttccct
     3781 gaccgaagtg tggggcaaag gcggcgcggg cggcgcggat ttggcgcgca aagtcgtcaa
     3841 tgccatcgac aaccaaccta ataacttcgg tttcgcctac gatgtcgagt tgggcatcaa
     3901 agacaaaatc cgtgcgattg cccaaaaagt gtacggcgcg gaagatgtcg atttcagcgc
     3961 ggaagcgtct gccgaaatcg cctcgctgga aaaactgggc ttggacaaaa tgccgatctg
     4021 catggcgaaa acccaatatt cattgagcga caacgccaaa ctcttgggct gccccgaagg
     4081 cttccgcatc accgtacgcg gtatcactgt ttccgccggc gcgggcttca tcgttgcgtt
     4141 gtgcggcaat atgatgaaaa tgccgggcct gccgaaagtt ccggctgccg agaaaatcga
     4201 tgtggacgaa cacggcgtga ttcacggctt gttctgaacg gtttttgaaa ccggatgccg
     4261 tctgaagccg tttcagacgg cattttttcg gaacgcgggc ggcggtatgc tataattctc
     4321 cgttaaattt ctctattttc aggaaaaacc atgagtttga aatgcggcat cgtcggtttg
     4381 cccaacgtcg gcaaatccac cctttttaac gcgctgaccc aatcgggcat cgaagcggca
     4441 aactatccct tctgcaccat cgaacccaac gtcggcatcg tcgaagtgcc cgacccgcgt
     4501 atggcggagc tggcgaaaat cgtcaatccg caaaaaatgc agcccgccat cgtcgagttt
     4561 gtcgatattg ccggcttggt tgcgggcgcg agcaaaggcg aaggcttggg caaccggttc
     4621 cttgccaaca tccgtgaaac cgatgccatc gtcaacgtcg tgcgctgctt tgacgacgac
     4681 aacatcgtcc acgtttccgg caaagtcgat ccgattgccg acatcgaaac catcggcacc
     4741 gaattggcgc ttgccgactt ggcaagtgtg gaaaaagcca tcgtccgcga agaaaaacgc
     4801 gcccgatcag gcgacaaaga cgcgcaaaaa ctagtcgatt tgtgcaaaaa actgctgccg
     4861 catctggacg aaggcaaacc cgtgcgttcc ttcggtttgg acgcggaaga acgcgcgctg
     4921 ctgaagccgc tgttcctgct gaccgccaaa cctgcgatgt atgtgggaaa cgttgccgaa
     4981 gacggttttg aaaacaaccc gcacctcgac cgcctgaaag aattggcggc aaaagaaaac
     5041 gcccccgtcg ttgccgtctg cgccgcgatg gagagcgaaa tcgccgaatt ggaagacggt
     5101 gaaaaagccg aatttctcgc cgaaatgggc ttggaagaac cgggcttgaa ccgcctcatc
     5161 cgcgcgggtt acgacctttt gggactgcaa acctacttca ccgccggcgt gaaagaagtc
     5221 cgcgcgtgga cgatacacaa aggcgacacc gcgccgcaag ccgccggcgt gatccatacg
     5281 gattttgaac gcggcttcat ccgcgcccaa gtgattgcct acgatgattt tgtctcgctc
     5341 ggcggcgaag ccaaagccaa agaagccggc aaaatgcgcg tggaaggcaa ggaatacgtc
     5401 gtgcaggacg gcgacgtgat gcactttttg tttaacgtgt aacccaaatg cggcaggttt
     5461 tcagacggct ttgccggaaa tgccgtctga agccggtttt ggtggttttc gacgttccca
     5521 taccgccgga atgcagccgc atcaaaataa aatcccgccc gcatttccga tttgccctcc
     5581 ccgattcctg caaaacaaac cgcctgccct gccgttacgg gaagccgtcc ggtattccga
     5641 atatcccgaa ccccgataca aaatgacctt tcagacggca tttgcacagc ccgccgcgtt
     5701 tcaagtaaaa acattatgag ccaagcctta ccctaccgcc cggacatcga cacattgcgc
     5761 gccgccgccg tcttgtccgt catcgtgttc catatcgaaa aggattggct gccgggcggg
     5821 tttctcggtg tcgatatatt ctttgtgatt tcaggctttt tgatgacggc gatcctcctt
     5881 cgcgaaatgt ccggggggcg tttcttcctc aagacatttt atatccgccg catcaaacgg
     5941 attttgcccg catttttcgc cgtattggcg gcaacgctgg caggcggctt ctttttattc
     6001 accaaagatg atttctttct tttgtggaaa tccgcgctga ccgccttggg tttcgcctcc
     6061 aacctgtatt ttgcaagggg gaaggattat ttcgatcccg cgcaggaaga aaagcccctg
     6121 ctgcacatct ggtctttgtc ggtcgaagaa caattttact ttgtctttcc gatattgctg
     6181 ttgcttgtcg cccgcaaaag cctgcgcgta cagttcggct tcctcgccgc attgtgcgcc
     6241 ttaagccttg ccgcttcctt tatgccttcc gcgctcgata aatattacct gccccacctg
     6301 cgcgcctgcg aaatgctggt cggatcgctg accgccgtgc ggatgcggta ccggcaacag
     6361 cggaatcccg ccgtcgggaa acggtatgcc gccgtcggcg cattgttttc cgcgtgcata
     6421 ctgtccgcct gcctgtttgc ctattcggaa caaaccgcct atttcccggg ccccgccgct
     6481 ttgattccct gtctggctgt tgccgcgctg atttatttca accattacga acacccgctt
     6541 aaaaaatttt tccaatggaa aatcaccgtt gccgccggtt tgatttccta ttcgctttat
     6601 ctgtggcatt ggccgatatt ggcctttatg cgctatatcg gcccggacaa cctgccgcct
     6661 tattcgccgg cggcagcgat cgtcctgacc ctggcgtttt ccctgatttc ttatcactgc
     6721 atcgaaaagc cgtttaaaaa atggaaaggc tcgttcgcac aatccgtttt atggatttat
     6781 gccttgccta tgctcgtttt gggggcgggc tcgtttttcg cgatgaggct gccgtttatg
     6841 gcgcaatacg accgcttggg gctgacgcgt tccaacacct cctgccacaa caataccggc
     6901 aaacaatgcc tgtgggggga tacggaaaaa cagccggaac tgctggtttt gggcgactcc
     6961 cacgccgacc attacaaaac attcttcgat gccgtgggca aaaaagaaaa atggtccgcc
     7021 actatggttt ccgccgacgc ctgcgcctat gtggaaggct acgcgtcccg tgtgttccaa
     7081 aactgggccg cctgccgcgc cgtttaccgc tatgccgaag aacacctgcc ccggtatccg
     7141 aaagtggttt tggcgatgcg ctggggcagc cagatgcccg aaaacagccg ctcccttgcc
     7201 tacgatgccg gttttttcca aaaattcgac cgtatgctgc acaaactctc atccgaaaaa
     7261 caagccgttt acctgatggc ggacaacttg gcttcgtctt acaacgtcca gcgcgcctat
     7321 atcttgtctt cacgcatacc gggttgccgc caaacactgc gcccggacga cgaaagcacc
     7381 ctgaaagcca atgcccgcat cagggaattg gcagccaaat accccaacgt ctatattatt
     7441 gatgccgccg cctatatccc cgcagatttc caaatcggcg gattgccggt ttactcggac
     7501 aaagaccaca tcaaccctta cggcggcaca gaattggcga agcgtttttc cgaaaaacaa
     7561 aggtttctcg atacgcgcca taaccattga ttcgcttaaa tttgttacaa tcggcggttt
     7621 gcaaaaccgc taattttttt gaaagagacc gatgagcgtc atccaagacc tgcaatcgcg
     7681 cggccttatc gcgcaaacca ccgacatcga agccttagac gctttgctga acgaacaaaa
     7741 aatcgccctt tactgcggtt tcgacccgac agccgacagc ctgcacatcg gacacctgct
     7801 gcccgtattg gcattgcgcc gcttccaaca ggcggggcat acgccgattg cactggtggg
     7861 cggcgcgacc ggtatgatcg gcgaccccag cttcaaagcc gccgaacgca gcttgaattc
     7921 cgccgaaacc gttgccggct gggtaggaag catacgcagc caattaaccc ctttcttgag
     7981 ctttgaaggc ggaaacgccg ccattatggc gaacaatgcc gactggttcg gcagcatgaa
     8041 ctgcctcgac ttcctgcgcg acatcggcaa gcatttctcc gtcaacgcca tgctgaacaa
     8101 agaatccgtc aaacagcgca tcgaccgcga cggcgcaggc atttccttca ccgagttcgc
     8161 ctattccctg ctgcaaggct acgacttcgc cgagttgaac aaacgccacg gcgcggtttt
     8221 ggaaatcggc ggttccgacc agtggggcaa catcaccgcc ggtatcgacc tgacccgccg
     8281 cctgaaccaa aaacaagtgt tcggtctgac cctgcctttg gttaccaaat ccgacggtac
     8341 caaattcggc aaaaccgaag gcggcgcggt gtggctgaac gcgaaaaaaa cctcgccgta
     8401 ccagttctac cagttctggc tgaaagtcgc cgatgccgat gtgtataaat tcctgaaata
     8461 ctttaccttc ctgtccatcg aagaaatcgg tgtcgtcgaa gccaaagaca aggcaagcgg
     8521 cagcaagccc gaagcgcaac gcatcctcgc cgaagaaatg acccgcctga ttcacggcga
     8581 agaagccctt gccgccgcgc aacgcatttc cgaaagcctg tttgccgaag accaaagccg
     8641 gcttaccgaa agcgacttcg agcagctcgc cctcgacggc ctgcctgcat ttgaagtttc
     8701 agacggcatc aacgccgtcg aagccttggt caaaaccggc ttggcagcgt ccaacaaaga
     8761 agcgcgcggc tttgtcaatg ccaaagcggt tctgctcaac ggcaaaccgg ctgaagccaa
     8821 caaccccaac cacgccgccg aacgccccga cgatgcctat ctgttgatag gcgaatacaa
     8881 acgtttcggc aaatacacca tcctccggcg cggcaaacgc aaccacgcgc ttttggtttg
     8941 gaaataatcc gattgccgca gaaatgccgt ctgaagcttt cagacggcat ttttatcaaa
     9001 tgcaaaacac cctgcgcctg ccgatatgtc gtcatttcca tgcaggcggg aattcaaact
     9061 tgtccgcacg gaaacttatc gggcaaaacg gtttcttcag ttctacgttc tagatgcccg
     9121 ctttcg
//
LOCUS       DAWTHD010000090         9014 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWTHD010000090 DAWTHD010000000
VERSION     DAWTHD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.090"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            129..619
                     /locus_tag="WM704_001689"
                     /pseudo
     CDS             129..619
                     /locus_tag="WM704_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            656..922
                     /locus_tag="WM704_001690"
                     /pseudo
     CDS             656..922
                     /locus_tag="WM704_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1424..2047
                     /locus_tag="WM704_001691"
     CDS             1424..2047
                     /locus_tag="WM704_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9341060.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            2159..3541
                     /locus_tag="WM704_001692"
     CDS             2159..3541
                     /locus_tag="WM704_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9341061.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            3545..4069
                     /locus_tag="WM704_001693"
     CDS             3545..4069
                     /locus_tag="WM704_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFA9341062.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQNQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            4442..5365
                     /locus_tag="WM704_001694"
     CDS             4442..5365
                     /locus_tag="WM704_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9341063.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLTLGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRPFKRR"
     gene            complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="WM704_001695"
     CDS             complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="WM704_001695"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFA9341064.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNRAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="WM704_001696"
     CDS             complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="WM704_001696"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFA9341065.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
BASE COUNT         1826 a         2279 c         2703 g         2206 t
ORIGIN      
        1 gcaagcgtag gtcggatact tgtatccgac aaaaccttta acattcccat cattgcaatc
       61 cattgcagca atgtccaaaa tgtcgaattc aagaatccgg cctacaaaat cattccgagc
      121 ataatactat gaaataccgt cgtttttacc gcaatggcgg cacttacttt tttacggttg
      181 taaccaataa acggcagaag attttgaccg atgatgcggt gcgtttggct ttacggcagg
      241 cggtaatggc ggtgcgcgaa cggtatccgt ttgaaatttt ggcatgggtg ttgatgcccg
      301 atcatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg gaacgctggc
      361 ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt tggcaaaacg
      421 cttttgggaa catactatcc gcggtgaggc cgattttgcc tgccattttg attatctgca
      481 tttcaatccg gtcaaacatg gctatgtagg acaaatttcc gattggcggt tttctacgtt
      541 tcaccgttat gtcaaacagg gtatttatcc gcataattgg ggtggcggca atgcggactt
      601 ttctattgaa tacgattgaa gtaatgtcgg attcgagaat ccgacctacg gaaaactgaa
      661 agagcatcgg ctgctggacg gcattatgcg caaagcccgc cgcaaccgcc cgctgccgga
      721 ggcgcaaacc aaacgcaacc gatatttgtc gaagacccgt tatgtggtcg aacaaagctt
      781 cggtacgctg caccgtaaat tccgctatgc ccgggcggcc tatttcggac tgattaaagt
      841 gagtgcgcaa agccacctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct
      901 aagtgcgccc gctgccgcct aaaaggcggc ccggatgcct gattatcggg tattcaaggg
      961 ggattaaggg ggtatttggg tagagttagg agtgattggt ggcggaaaca gccgaaaatc
     1021 tgtgtcaggg tttcagctgt cgggagggaa gggaattttg caaaggtctc aagatatagt
     1081 ggattaaatt taaaccggta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc
     1141 cactatattt ccgcctgctg ccgcccgaaa agcgtaatgc gctgatggtg ctgctgcaca
     1201 atccggacga cggataaccg gtgaaaacgg gaaatgcccg ggcggtgtcg tctgtgcctg
     1261 tttggacaag cattcttgag ggcggtaaaa tcaaggtttg cccgaaaagg gcagggcgat
     1321 atgccgcgct tggattttgc gcgcggtttt tcgcgttgga agcaatcatg tctgtttgaa
     1381 tatttaatgc cgtctgaaac gtccggacgc acccccgttc cttatgaaaa aaccgaccga
     1441 taccctaccc gtcaatctgc aacgccgccg cctgctgtgt gccgccggcg cgctgttgat
     1501 cagcccgctg gcgcacgccg gcgcgcaacg tgaagaaacg cttgccgacg atgtggcttc
     1561 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgttcg acaatccgaa
     1621 agagggcgaa cgttggttgt ccgcgatgtc ggcacgtttg gcaagattcg tccccgacga
     1681 gggggagcgg cgcaggctgc tggtcaatat ccaatacgaa agcagccggg ccggtttgga
     1741 tacgcagatt gtgttggggc tgattgaagt ggaaagcgcg ttccggcagt atgcgataag
     1801 cggggtcggc gcgcgcggtc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
     1861 accggcgcac aacctgttcg atatccgtac caacctgcgt tacggctgca ccatcctgcg
     1921 ccattaccga aaccttgaaa agggcgacat cgtccgcgca cttgcccgct tcaacggcag
     1981 cctcggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     2041 gcgttgattt tgaacccgtg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     2101 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aattatttat
     2161 ggcaagagac aaccgcatcc aaatgtttcc gcacgaatgg cgcgccagta cgacgctttc
     2221 cggcgtgtac gcgctgcgta tgctgggtat gttcctcgtg ctgcccgttt tggcaatata
     2281 tgccgcctcg ctgcccggtg cggaagacaa taaaacgctg gtcgggctgg caatgggcat
     2341 ttacgggctg acgcaggcat tgctgcaact gcctttgggt atcgcttccg acaagttcgg
     2401 gcgcaagaaa accatttatg tcgggctggt cgtgtttgcc gccggcagct tccttgccgc
     2461 cgccgccgat acgctgccca tgctggttgc cgcgcgcgcc atacagggcg cgggggcggt
     2521 cagtgctgcc gtaaccgcgc tgcttgccga cttgacgcgc gacggcgtgc gtacccgcgc
     2581 gatggcgatg atcggtttga gtatcggtct gacgttttcg gtcagcctgg tggttgcgcc
     2641 gatgattgcc gatgtggccg gcgttcgcgg actgtttatg ctgaccggca ttctgaccgt
     2701 catcagcatc ggcgtagtgg cgtggatgac tcctgatccc gaagtttcca agctgcatga
     2761 ggacacgcag gcgcagcctt cgcgcatagg agaagttttg aaaaaccgta ggctgctgac
     2821 gctcgatttc ggcattttcg ccctgcacgc cgcgcaaatg gcattgttta ccgcgctgcc
     2881 tttcgcgatg acccggctcg gtttggaaaa aatccagcat tggaaagtat atctgccctc
     2941 gaccattacg ggcttggtgg tgatggttcc gctgattatc gtcggcgaaa cgcgcaacaa
     3001 gctcaaacag gtttttgttt tgggcatcgt ctgcatcgcg gcggcgcagc ttggtttgct
     3061 gtccggaatg cactcgattt ggctgattac cgcttatctg gttgtttact ttatcggttt
     3121 taatgtgttg gaagcgagcc tgccgtctat ggtttccaaa atcgcgccgt ccgacctgaa
     3181 gggtacggcg atgggcgtgt acaacacgat gcagtcgctc ggactgtttg ccggcggcgc
     3241 ggcaggaggc ttgctgtttc aaaaatacgg ctttgccggc gtgtttgcct tttgcagcat
     3301 attgatgctg ctgtggctgg tgattgccgt tttatcgccc gcgcccaagc ccgtcaaaaa
     3361 cctcagttac cctgtcggcg gcgtgtggca gggcaatcgg gacggtttgc aacgcgccct
     3421 gttgcagttg gaaggcgtgg aagacatcgg tttcagtttc gacgggcaga ccgtctatct
     3481 taaagtgttg cagaagggtt tcgatcaggc tgccgctgaa aaaatcatta caggagttta
     3541 aaaaatgtca ttgaacaaag tcatccttat cggccgcctc ggacgcgatc ccgaagtgcg
     3601 ctatatgccc aacggcgagg cagtgtgtaa tttcagcgtc gccaccagcg aaacttggaa
     3661 cgaccgcaac ggccagcgcg tagagcgcac cgaatggcac aacatcacga tgtaccgcaa
     3721 actggcggaa attgccgggc aatacctcaa aaaaggcggg ctggtttatc tggaaggcag
     3781 aatccaaagc cgcaaatacc aaggcaaaga cggcatcgaa cgcaccgctt acgatattgt
     3841 cgccaatgaa atgaaaatgt tgggcgggcg caatgaaaac agcggcggcg cgccttacga
     3901 tgaaggttac ggtcagaatc aggaggctta ccaacgcccc gctcagcaaa gtcggcagcc
     3961 cgctcccgac gcgccgtccc atccccaaga agcacccgcc gcgccgcgcc gccaacccgt
     4021 gccggccgcc gccccggtcg aggacattga cgacgacatc ccgttctgaa ttttacggcc
     4081 ggacatcctc gcggagggaa gtcataaagg acggaaaaac cttaaacctt acggggcaag
     4141 gtttctcttt tttgcatttg ggccggcttg ttgccgcccc ctgcgttggg cgtaacgttg
     4201 cgattgcgcc gttcgggcgt tttgaggcaa gcgatgcctg ccgcactccc ccgctgtttt
     4261 cagacggcat tttaaaattg aggcggtgtt ttcaaatagt gcgaagcaat agtcgggctt
     4321 cagccagttt gtttgaaatc aaagggttgg aatcaaacgc caaacgcttg aaccatcatt
     4381 gcccgatgcg gcaaaccggc atcgtccggc aatacggttg tttaaaaata gggaaatcag
     4441 gatggaaaac caaaggccgc tcctaggctt cgcgttggca cttttggcgg cgatgacgtg
     4501 ggggatgctg ccgattgccg tgcggcaggt attgaagttt gtcgatgcgc cgacgctggt
     4561 gtgggtgcgt tttacagtgg cggcggcggt attgtttgtt ttgctgacat tgggcgggcg
     4621 gctgccgaag cggcgggatt tttcttggca ttcattcagg ctgctgctgc tcggcgtgac
     4681 gggcatttcg gcaaactttg tgctgattgc ccaagggctg cattatattt cgccgaccac
     4741 gacgcaggtt ttgtggcaga tttcgccgtt tacgatgatt gttgtcggcg tgttggtgtt
     4801 taaagaccgg atgactgccg cgcagaaaat cggtttggtt ttgctgcttg tcggtttgct
     4861 tatgtttttt aacgacaaat tcggcgagtt gtcgggtttg ggcgcgtatg cgaagggcgt
     4921 gttgctgtgt gcggcaggca gtatggcctg ggtgtgttat gccgtggcgc aaaagctgct
     4981 gtcggcgcaa ttcggtccgc aacagattct gctgttgatt tatgcggcaa gtgccgccgt
     5041 gttcctgccg tttgccgaac cggcacacat cggaagtttg gacggtacgt tggcgtgggt
     5101 ttgttttgtg tattgctgct tgaatacgtt aatcggttac ggctcgttcg gcgaggcgtt
     5161 gaaacattgg gaggcttcca aagtcagcgc ggtaacaacc ttgctccccg tgtttaccgt
     5221 aatattttct ttgctcgggc attatgtgat gcctgatact tttgccgcgc cggatatgaa
     5281 cggtttgggt tatgtcggcg cactcgtcgt ggtcgggggt gcggttacgg cggcggtggg
     5341 ggacaggccg ttcaaacgcc gctagttcgc aggcaacgga aaatgccgtc tgaacgaggc
     5401 ttcagacggc attttatttg agggaaggat tagcgcggat gaaccatatc ggcagggacg
     5461 accagttcgt caaactcttc gcccgtcagc aagcccaacc caacggcagt ttcgcgcagc
     5521 gatttatcgt ttttgtaggc ggttttggcg actttggcgg cgttttcgta gccgattttg
     5581 cggtttaacg cagtaaccag catcagggaa tggtgcagga aatagtcgat tttttccggt
     5641 acgggttcga tgccggcggc gcagttttcg ttgaagctgt tgcacgcgtc gcccaagagg
     5701 cggatggatt gcaagaggtt gtaggcgata acgggcatat agacgttcag ctcgaaattg
     5761 cccgacgcgc ccgccatacc gatggtaacg tcgttgccga acacttggca gcacaccatc
     5821 gtcatcgctt cgcattgggt cgggttgact ttgcccggca taatggacga acccggttcg
     5881 ttttcgggga ttttgatttc gcccaagccg cagcgcgggc cgcttgccag ccagcggatg
     5941 tcgttggcga ttttgttcag gcttgccgcc agcgttttca atgcgcccga agcggcaacg
     6001 gcggcatcgc gtccgcccaa ggcttcaaat ttgttcggcg cgctgacaaa cggcaggccg
     6061 gacagttcgg cgagtttggc ggcggctatt tcagcgtatt cgggatggct gttcaaaccc
     6121 gtaccgaccg ccgttccacc caaggcgagt tcgtacaaac ctttgagcgc gtcgttcaga
     6181 cggcctaagc tgtggtcgag ttgggaaacg tagccggaaa attcttgtcc caaagtcagc
     6241 ggcgtcgcat cctgcaagtg ggtacggccg attttgacga tgggggcgaa ggcttgggct
     6301 tttttgtcca atgtatcgcg caaggctttt accgccggaa taaggtggcg gttgatttca
     6361 atcgcggcgg cgacgtggat ggcggtcggg aacgcgtcgt tggtcgattg cgcgcggttc
     6421 acatggtcgt tggggtggac gggccggtac gccgccaaac ccgtacctgc aatttcgttg
     6481 gcgcggtttg ccagcacttc gttcatgttc atattggact gcgtaccgga accggtctgc
     6541 caaaccacta aggggaactg cccgtcgagc ctgccgttca gtacatcatc cgccgcctgc
     6601 gtaatcaaat ccgcctgttc gggcttaatc ctgccgaggg aaacattggt cgcggcggcg
     6661 gcttttttca ccaacgccaa agcgtaaatc agcggctgcg gcagggtttc gccgccgatt
     6721 ttgaaattgt tgcggctgcg ctgggtctgc gcgccccaat aggcttcgga cgggacttcg
     6781 acattgccca tcgtgtcgtg ttcggtgcgg gtgttcatac gtttctcctt ttgaaatgtg
     6841 aataagagtg attcgcaaat attataatgg agattggcgg aatgaggaag catcaaggcg
     6901 ggagggcaaa aaaatgccgt ctgaaaagca ttcagacggc atttgatgcg gcggctgagg
     6961 tttacagcac ggatttcacc gtatcaacca cattgtccac ggtaaagccg aatgctttga
     7021 acagcagatc ggcaggggcg gattcgccga agcggttgat gccgacgact gcgccgttca
     7081 gtccgacata tttgtaccag ccgttggtgt gtccggcttc tacggcgatg cgcggcaggc
     7141 cttccggtag gacggcggct tgataggcgg cgtcttggcg gtcgaagacg ctggtggacg
     7201 gcatggaaac gacgcgcacg gcaatacctt gtcctgccaa tactttttgc gcttctacag
     7261 ccaatcctac ctcggaaccg gtggcaatga tgacggcttg ggcgttgcct tgggcttcac
     7321 tgattacgta agcaccgcgt ttgatgtcgt tcagttgttg ctcgctgcgt gcttggaatt
     7381 tcaggttttg acggctgaaa atcaggcagg acgggtggtc ttcggctttt gccgcttctg
     7441 cccaagcgac cagagattct gcggtgtcgc acggccgcca tacgtccata ttcggaatca
     7501 ggcgcagggt ggcggtttgc tcgataggct ggtgggttgg gccgtcctcg cccaaaccga
     7561 tggaatcgtg ggtgaatacg aagacggggt tgattttcat cagtgccgcc atgcgtaagg
     7621 cgttgcgttc gtattcgctg aacatcagga aagtcgcgcc gaagggtttt acaccgccgt
     7681 gcaataccaa accgttcata atcgcaccca taccgaactc gcgcacgccg tagtggatgt
     7741 agttgccgcc tttgtcgcgg gtaacggaga cgctgtttga ccagtcggtc aggttggacg
     7801 gggtcaggtc ggcagaaccg cccaccagtt cgggcagctc ttttgccaag atttcgatgc
     7861 tgttttggct ggctttgcgg gtggcgacgg tttccgcttt ggcgcacact tctttcaatg
     7921 ccgtctgaac gtactcgtca aagttttccg gcagcttttt atccatgcgg cgcacgaatt
     7981 ctgcggcttc ggcaggatat ttggcttgat attgcgcgaa cagttcgttc catccggctt
     8041 ccagtttcgc gcctttttct ttggcattcc acgcatcgta aatttcttgc gggatttcaa
     8101 acgcaggata agcccaaccc aaatgtttgc gcgtggcttc gatttcgtcc gcacccaaag
     8161 gcgcgccgtg ggttttgtgg ctgccttctt tgttggcgct gcctttgccg attaaggttt
     8221 tgcagcagat gatggacggc ttgccggttt cggcgcgggc ggcttcgatg gccgtctgaa
     8281 tggcggcggt gtcgtggccg tttacgttgg gaacgacgtg ccagccgtag ctttcaaagc
     8341 gttgcgggat gttttcggta aaccagccgt ccactttgcc gtcgatggag atattgttat
     8401 cgtcatacaa aacaatcagt ttgcccaagc ccaaggtgcc ggcgagtgag caggcttcgt
     8461 gcgatacgcc ttccatcagg cagccgtcgc ccataaagac gtaagtgtag tgatcgacga
     8521 tgttcaaacc gtctttatta aattcggcgg caaggatttt ttctgccaat gccatgccca
     8581 ctgcgttggc aataccttgc cccaacgggc cggtcgtggt ttccacgccg tcggtgtagc
     8641 cgtattcggg atggccgggg gttttgctgt gcagctggcg gaagtttttc agatcttcaa
     8701 tgctcaggtt gtagccggtc aggtgcagca ggctgtacaa cagcatagac gcatgaccgt
     8761 tggagaggac gaagcggtcg cggttgtaga atttgggatt ggcgggattg tgattgagga
     8821 atttcgtcca caatgtttcc gccatttccg ccatacccat aggcgcgccg gggtggccgg
     8881 aattggcttt ttgaacggca tcggccgaga ggaagcggat tacgtttgcc agttgagaca
     8941 ttttgtattt tccttgctgg tgtttcggat aagtggataa tcggaaggcg ttgattatcg
     9001 cccgattcgc ttat
//
LOCUS       DAWTHD010000091         8491 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWTHD010000091 DAWTHD010000000
VERSION     DAWTHD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.091"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(210..3062)
                     /gene="gcvP"
                     /locus_tag="WM704_001697"
     CDS             complement(210..3062)
                     /gene="gcvP"
                     /locus_tag="WM704_001697"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFA9341066.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3093..3452)
                     /locus_tag="WM704_001698"
     CDS             complement(3093..3452)
                     /locus_tag="WM704_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341067.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3400..3630
                     /locus_tag="WM704_001699"
     CDS             3400..3630
                     /locus_tag="WM704_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFA9341068.1"
                     /translation="MISACPGFIRSRFGRSVMPSEPAGRLSQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3623..4264)
                     /locus_tag="WM704_001700"
     CDS             complement(3623..4264)
                     /locus_tag="WM704_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFA9341069.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4254..4805)
                     /locus_tag="WM704_001701"
     CDS             complement(4254..4805)
                     /locus_tag="WM704_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFA9341070.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4809..5327)
                     /locus_tag="WM704_001702"
     CDS             complement(4809..5327)
                     /locus_tag="WM704_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFA9341071.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGKNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5327..6988)
                     /gene="pqiB"
                     /locus_tag="WM704_001703"
     CDS             complement(5327..6988)
                     /gene="pqiB"
                     /locus_tag="WM704_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFA9341072.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6981..8441)
                     /locus_tag="WM704_001704"
     CDS             complement(6981..8441)
                     /locus_tag="WM704_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFA9341073.1"
                     /translation="MGLSAGGAAAQKIFLPDTKGLFILPNLPVLHPDWVYRFFSFIMP
                     PFIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRTDVPQLDKGEAAFCPR
                     CGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGNN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVVLYFPA
                     NILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     AARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
BASE COUNT         1843 a         2187 c         2458 g         2003 t
ORIGIN      
        1 attgagaaag tgtgggcgaa tattaagcgg tatctgcgaa ccgttttgtc tgattacgcc
       61 cgatttgacg atgcactact gtcctatttt gattttaatt gactataaag ttatagtcga
      121 gatacgtgta tcggtgcaac cggtctgccg ttgacgaaaa tgccgtctga aagtgtttca
      181 gacggcattt gtcttcgggg tttcaacagt cagtcttcat aattttccat cggcgggcag
      241 ctgcacacga ggttgcggtc gccgtacacg tcgtccacgc ggttgacgaa gggccagaac
      301 ttgtgttcgc gcacgaaggg cagcgggaag acggcttctt cgcgagagta cggatgcgcc
      361 cattcgccgg ttatatctgc ggcggtgtgc ggcgcgttga ccagtgggtt gtcgtctttc
      421 ggccattcgc cgtcgatgac tttctgcact tcgcggcgaa tggatttcag ggcggcgatg
      481 aagcggtcga gttcggcttt gctttcgctc tcggtcggtt cgatcatcag cgtgccggca
      541 acagggaagg aaaccgtcgg cgcgtggaag ccgtagtcca tcaggcgttt ggcgatgtcg
      601 gtttcggtga tgccgctttc ggctttgagc ggacgcaaat cgacgatgca ttcgtgcgcg
      661 atgcggccgt ttttgcctgt atacagaatc ggatagtctt cgctcaagcg tttagcgaca
      721 tagttggcgt tgagcaatgc ccagcgtgtt gcctgttcca tgccttgttt gcccatcatg
      781 gtcaggtaca tccaagtaat cggcaggatg gatgcagaac cgaaggctgc ggcggcaacg
      841 gaggtttgcc cggcacttgc gctgtgggtg tcggtcaggg tatgccccgg tgcgaacggg
      901 gcgaggtggg ctttcaagcc aatcggaccc atgcccgggc cgccgccgcc gtgagggata
      961 cagaaggttt tgtgcaggtt catgtgcaac acgtccgcgc cgacttcggc gggctgcatg
     1021 atgccgattt gggcattgag gtttgcgccg tccatgtaaa cctgtccgcc gttttcgtga
     1081 atgatccggc agatgtcgcg gatgccttct tcgtacacgc cgtgtgtgga cggataggta
     1141 atcatgatgg cggacaaggc atcgcggtgt tgttcggctt tggctttcaa atcgtcaata
     1201 ttgacgttgc cgtgttcgtc ggtatcgacg acgacgactt tcaaaccgag catggcggcg
     1261 gtggcggggt tggtgccgtg ggcggatttg ggaatcaggc agatgttgcg gtgtgcttcg
     1321 ccttgggctt cctgataacg gcggatggcg agcataccgc tgtattcgcc ttgtgcgccg
     1381 gagttgggct ggaaggaaat cgcgtcaaag ccggtgatgg ctttcaggct gttttccata
     1441 tcagcaagca attcgcggta gccggcggtt tgcgcttcgg gagcgtaagg gtggatgtcg
     1501 gagaactcag tccaagtaat cggcaacatt tccgcagtcg cattgagctt catggtgcag
     1561 ctcccgagcg aaatcatgct gcggttcata gccaagtcgc ggtcttcgag ttttttcagg
     1621 tagcgcaaca tttcgtgttc ggtgtggtaa ctgttgtaca caggatgttg cagaatgtcg
     1681 tcttgacgca gtaattcggc gttcagacgg cctttgacat catcggcaaa tgtgaacgta
     1741 tccttgccgg taaacgcgcg gtacaaatcg gccaaatctt cgtataccga cgtttcatgg
     1801 aatgcagccg caacttgagt attgttgacg cggcgcaggt tgtaacccga ctccaaagca
     1861 gcggcaaaca cttggtctgc tttctctttg ctgccgaaat cgacggtaac ggtatcgaag
     1921 aagacttcgt gaaccacttt gaggccgtct gaaaccagcg cgtcggcaaa gacggaagcc
     1981 agtgtgtgaa tgcggttggc gatgcgtttc acgcctttgg ggccgtgata aacggcgtac
     2041 atgcccgcca agttcgccag caatgcctgt gcggtacaaa tattggatgt cgctttttcg
     2101 cggcggatgt gttgttcgcg ggtggacaaa gccatgcgca aggcaggttt gcccgatgcg
     2161 tctttggata cgccgatgat gcggcccggg gcggagcgtt tgaactcgtc tttaaacgcg
     2221 aaataagcgg cgtgcggacc accaaagccc atcggtacgc cgaaacgttg ggtgttgccc
     2281 aaagcaatat ctgcacccaa ttcagccgga gacttcagca aaaccaagct catgatgtcg
     2341 gcggcaacag caacgattgt gcctttggct ttcagacggc cgataacgtc ctgcaagtct
     2401 tgcacgtcgc cgtctttacc gacgtattgg aacagcgcgc cgaagtattc gccttcatcc
     2461 gcttgggcaa aatcgctgac caccagctcg aagccgaaat atttggcgcg ggttttcatc
     2521 acgtccaaag tttgcggata cacgcgcgca tccacaaaga aacgctcgga tttcactttg
     2581 cccacgcggt gcgccatcgc catcgcttcg gcggcggcgg tcgcttcgtc caacaaagac
     2641 gcgcccgcca cagggaaacc ggtcaaatcg atgcacacct gctggaagtt caacaacgct
     2701 tccaaacgac cttgggcaat ctccgcctga tacggcgtgt aggcggtgta ccagcccggg
     2761 ttttccaata cgttgcgcaa aatcacgttc ggcacgcggg tcgggtaata acccaagccg
     2821 atataggact tgttgatcac gtttttcgac gcgatgcctt tcaatttcgc caatgcgtcc
     2881 gcctcggtca gggcttcggg caaatcgagt tcggacggca tacggatgct ttgcggcaag
     2941 gtgttgccga caaagtcgtc catgctcttc tcgccgacag cggcaagcaa cgccgcttcg
     3001 tcgccgaaac tcaaatggcg cgcggcaaat tcgttggggt tgaacagttc ggacagtttc
     3061 atttctactc cttcgggttt gtagggctgc catcaggcag aatttttaga catcggatga
     3121 ttcgggcaag ggtggcacaa aaggcgggat aatggcggac aacgcgccag aatgcagcag
     3181 gtcgggacaa gcagcgccac aagcggcagc gataagggaa agccgccgcc caaaaccatg
     3241 aaacctgcag gcaccagcca agccaaggga tgagccggaa gcggaaaaac aacattgagc
     3301 agaaccaaaa tcacgacaaa cagcggcagc cccgccatca ggttggcacg cctgtatgtt
     3361 gcgtacgggt cggatgcttc ttcaggttcg caatattcca tgatttcagc ctgtccgggc
     3421 tttatccgca gcaggttcgg acggtcggtc atgccgtctg aaccggcagg ccgtctttct
     3481 caaaaaacgc cataccgctg caacttcatg cggctgtatg gcgtaggttt tgcacttcag
     3541 acggcatatg cgtcccgaag cagtttggtg tcccagtcgg ttcatgatta tataaacaac
     3601 ttgggtgcgg ataaaccttt gtttatctaa acccatgttt tctcaaaaat tgattcaaat
     3661 tttcggcgaa tttccgatag cctttgccgt tggcgtggat ttggtcggat ttcagattat
     3721 tattgcccaa aatttccgcc cacgcgccgc cgaacaacgg aatgccgtat tcctcggaca
     3781 aatcctcata cagcggatgg tcgctcaaat gcccgaacaa cgcgcccagt gtgatgtgcg
     3841 gcacgccgac gaggacggcg ggaatgtttt ccttttgcac ggtttcgatg attttcgcga
     3901 tattggcgcg ggtctgctcc tcgggaactt tgcgcagaaa gtcgttgccg cctatgccga
     3961 caatcacaag cttgggtttg cgtgccaaca gcgcgggcag gcgcgatagg gcttgcgcgg
     4021 acgtatcgcc cgatacgccg ccgttgacaa tattccaacc cgtcagtttt tgcagttgcg
     4081 cggggtagga ttcgccgggg tttgctccgt agccgaaggt gagcgaatcg cccaaggcaa
     4141 gcacggtgct tccttcggga attttggcgt gggttcgggc ggattttctg ccgcaggcgg
     4201 taagaagcaa cgcgcctgcg ccgaggagga aggttcttct gttcattggt ttttcagacg
     4261 gcatcgagcc atttcgggtg gtatttcagg gttttacggt aaaccggaca gctttcggcg
     4321 tgcgcgcctt tgaggtagcc ggtactcatc agaaattcgc cgacgatttc gccgccgacg
     4381 aatttgaaat gttttttaaa gagtttgacc cattcgtctt tgcttcgcgg atggtgcgcg
     4441 tcgagccagt tcttgaacga accgtgttct tgttgcaacg cttggatttg ccgtgcattg
     4501 aaaatggcgg catcgatttt cagacggttg cggacaatac ccgcatcggt aagcagccgt
     4561 tcaatatcgg cttcgccaaa tgcggcgacc gtatcgatgt cgaaaccttc aaatgccgtc
     4621 tgaaacgcct gccgcttctt cagcatcagc gtccaactta atcctgcctg attgatttcc
     4681 aacaccagcc gttcaaacag tccgttgtcg tccttaatcg gaaaaccgta ttgcgtatcg
     4741 tggtaatgtt tgttcgggtt gtcggtgttt tcgggaagtg aggcggcaaa ttcgcaatag
     4801 ttcacggttt actcgaccat ctgttgcgcc gcctgtttca gtccctgttc gagtgcggcg
     4861 gtcatggcgg cgtagccgtc accctgctgt tcggtttcga tatggaaggg tctgttctta
     4921 ccgtcgggta ggacggcgta gccgctgatg agggttttgc ccgtgtagct gccttggaat
     4981 gcgtcgatat agaccgtcca tttgtcggta ctgccgctgc gtgaggcagg aacaaaggtg
     5041 cgtgtgctgt ccaaacggtt gaatgcattg ctcaacgccg cttcgagcat atcgtccaag
     5101 gtgtctgccc aaacatggtt ttgtgcggtg ttgatgcggt aggggtcggt ttgatagacc
     5161 agtccgccgc gtttgagcgg ttcggcaaga cggacttcga cggcggtttc gccgccttgc
     5221 gttgcaggac ggatgtagcg gctgtcgggc aacacgaaat attgtgtgct ttgcacagta
     5281 ccgcaggcgg caagcgtcag ggcggcggcg atggggaaaa ggcgcattat cggcttcctt
     5341 tcgggatagg gtctttgctg ctgttgttga aaatcagtgc gttgggtttt tctttcaaag
     5401 tgttaatgac gggttgaacg tcttttaagg ttttgtccaa actttgcagc gtattttgta
     5461 cgtctccgta gataggcgat tgaggcgata cgccttgcag ggttatgcgc aactctttca
     5521 gagtttggtt cagttcgttc gggatgtttt gcgtctgcgg attgccgacc agtttgtcaa
     5581 tggagcttag ggcggcattg gcggatttga gtgcggactt gagttcggcg agcgagccgt
     5641 tcaattcggc aacggtttta tccaatggca gattgttgaa tttgtccagc aaatccgcca
     5701 atttgacctg caagtcgtcc aaaccgccgc cccgtgtggc gatgacggta tcgcctgcat
     5761 aaacggtatg cggtcgcagc ttgggcgagg cggaaggctg atcgttcaac tcaatcattt
     5821 tgccgccggt cagcaggttg ttgctggaga tggtggcggt caggcctttg tttaaggccg
     5881 tctggaattg ttgtttccaa tgctctttgc tttgctcgtc ggcattgatt tccaaacggg
     5941 aaggctcgat gcggatgcgt acgggaatcc agccgttttc aaacaggtgc aggctgtcat
     6001 tgcggtcaaa ataagggaca tcggaaacca tgccgacatt cagccctttg tattcgacag
     6061 gcgaaccgac ggtcagtccg cgcacggatt gtttgaaaaa cgcggtgtag tacagcgagc
     6121 ggtcgtcagg caggttggcg atttcgctgc ggctgtcgta aagcgtgaag ctgtcctcgc
     6181 ttttgacgtt tttactgttt ttggttttcg gcgaatcaaa tgaaatcgcg cctgacagca
     6241 gggcaggcag aggggcggaa ttgagtttga tgccgctgcc tgtggtttcg atattgatgc
     6301 cgctttccag ccaaaaacgg ctggcggaat gaatcagttt gtcgttgggg ctttggatga
     6361 agatggtgta atgcacgctt tggtcggacg ggtcgaaatg cgcgctttcg atttgcccga
     6421 ccataaagtt ttcatacaaa acagggctgt tgacgttgag gatgcggtcg tttttaccaa
     6481 tcaaattcaa gcgcagcccg ctttgcccga tggcggtaac gggcggaatg tcctgcactt
     6541 ggaacacgtc ttttgcctcg ccgcttttgc cgggtgtaaa ggcgatgtac gaacccgaaa
     6601 gcagcgtacc caaaccggtt acgccgcttt ggtcgatacg cggcttgacc acccagaact
     6661 gggtatcgct gcggatgagg ccggatacgt ccgcattgag ttgggcagta acttccacgc
     6721 ctttttggtc gtcgcgcagt ttgattcggg taacgcgtcc gacatcgatg ctcaatacct
     6781 taatgaccgt attgttgact tcgatgcctt ccgcgctgtc catcaagagc gtaaccacag
     6841 gccccctgtt gcggatttcc ttaacccaaa gccagccgcc ggcaatcagc gcgatcagcg
     6901 ggaccagcca gacggcggag aggaaggtgt tgtttttgcg gacgcgtgct tgagcgtgtc
     6961 cgtttggagg agggctgttg tcagtcatat ttttccgttt cgttgaaagc aatgccgtct
     7021 gaagcgcgtt tgtcccaaag caggcgcggg tcgaaataat aggcggacag catcgtcaaa
     7081 atcacgacca ggcagaaata gactgccgca ctgcccggaa tgacgcgcgc ggcataagtg
     7141 tggaacgaac acatcaaaat aataatcaca aaaatatcaa tcatcgacca gcggccgacc
     7201 gcttcggtga tgcggtagag gtgcgacaat ttctttgcgc ccgccggcaa agcgaaccgt
     7261 gccgccgcaa tcaaaaccga cattgccgca atcttcagca ccggcaccaa aatactcgcg
     7321 ctgaaaataa ccgccgcaat cagcctgtcg ccctcgtccc acatataagc gatgccgcta
     7381 aagatggtgt tggcctccgt ggcggcagga ttggacgaaa tcataatcgg caggatattg
     7441 gcagggaaat acaaaacaac cgccgccgtc agaaacgccg acgaaatact cagacttttc
     7501 ggccgtcggc ggtacagttc cgcgccgcac accccgcagg gggattcggc actgtcgcgg
     7561 aaatacaggc agcggctgca acaggttttg ccttccgatg ccgtctgaac cgcattattc
     7621 cccgtcagcc gcccgatttg gaaatacacc caatgctggg gaaccgatac cgaagtccga
     7681 atcagcataa ccgacagcgc gaacatcaga taaaacgccg gcccgaagcg aacctttgcc
     7741 acagacgaga gcttgatata cgccaccaga gtggaaacaa aaaacacatc caccatcatc
     7801 gcctgcctca agcgcaccat cacacgcgtt gccaaacgca gcgcaggata cgcctgtttc
     7861 cgtatcagcg cggcatagac atacaggcac agcagcagaa acagaaccgg cgcgccgaaa
     7921 gtcagcacaa acatcacttc ggccaaaaaa ccataatcct gaaacaccat caggcgcatc
     7981 atctcgggca gcgaaaggac ggatgccgca cccggtatcc cgacctcgat atacgtcata
     8041 ctgtaagcaa acgccatcaa aatcagcgaa gccgccgcat aggcgggcgg accggaaaaa
     8101 gggtggctgc ccaccctgaa gagtttgtgt ccgcaacggg gacagaacgc cgcctctccc
     8161 ttgtccaact ggggcacgtc tgtacggcag ccgcattccg ggcaatctac cgtatgcggc
     8221 ggcagggctt cgtcgcggca gttgccgcga aggctgtggg ggatattttc ggcaaacggc
     8281 ttcatcggga aagtggcaaa tataaatggc ggcattataa aggaaaaaaa gcgatacacc
     8341 caatcaggat gcaaaaccgg caggttcggc agtatgaaca aacccttggt gtccggcaaa
     8401 aatatttttt gagcggctgc ccctccggcc gacaagccta tttgataaaa ccccgatttt
     8461 gtaaataatc ataaaatacc cgcttaaaaa t
//
LOCUS       DAWTHD010000092         8482 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWTHD010000092 DAWTHD010000000
VERSION     DAWTHD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.092"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(108..1325)
                     /locus_tag="WM704_001705"
     CDS             complement(108..1325)
                     /locus_tag="WM704_001705"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFA9341074.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1527..2255)
                     /gene="rph"
                     /locus_tag="WM704_001706"
     CDS             complement(1527..2255)
                     /gene="rph"
                     /locus_tag="WM704_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFA9341075.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2337..2801)
                     /locus_tag="WM704_001707"
     CDS             complement(2337..2801)
                     /locus_tag="WM704_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFA9341076.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3018..3794
                     /locus_tag="WM704_001708"
     CDS             3018..3794
                     /locus_tag="WM704_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HFA9341077.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNANRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4039..4893
                     /locus_tag="WM704_001709"
     CDS             4039..4893
                     /locus_tag="WM704_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFA9341078.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKIVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            4934..6142
                     /gene="pncB"
                     /locus_tag="WM704_001710"
     CDS             4934..6142
                     /gene="pncB"
                     /locus_tag="WM704_001710"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFA9341079.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6234..7952
                     /gene="argS"
                     /locus_tag="WM704_001711"
     CDS             6234..7952
                     /gene="argS"
                     /locus_tag="WM704_001711"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFA9341080.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
BASE COUNT         1937 a         2484 c         2181 g         1880 t
ORIGIN      
        1 tattttttgt tccaaagtca aaatatgccg tccgaacgtt cgggcggcag acaaaacggc
       61 actgcccgat aaaggcagtg ccgttgtccg ttttaaaccg tgaaacatca gcccaaatca
      121 aaggctttat gcaataccct ggttgccagt tccatgtatt tttcgtcaat caatacggaa
      181 actttgattt cggaggtgga aatcatttgg atgttgatgc cctcttcggc gagcgtgcgg
      241 aagattttgg cggctacgcc gacgtgcgaa cgcataccca aaccgactgc ggagactttg
      301 cacacggtgt cgtcgccgtc gatagaagct gcgccgatac tgtctttacg ttccgacagg
      361 atttccaaag tctgtttgta atcgccgcgc ggtacggtga aagaaaaatc ggttgtgcct
      421 tcgctgccga cattttggat aatcatatcg acttcgatgt tggcatctgc gaccgcgccc
      481 aaaatctgat aggcgacgcc gggcttatcg ggcacaccgc gcacgttgat gcgggcttgg
      541 tttttatcga atgcgatacc ggttacggca gctctttcca tgttgtcgtc ctcttcaaag
      601 gtaattaagg tgccgttgcc gccgtcttgc aggctgctca gtacgcgcag gcgcactttg
      661 tattttccgg cgaattctac tgaacggatt tgcaaaactt tcgaaccgag gctggccagt
      721 tcgatcattt cttcaaatgt aaccgtatcc atacggcgcg cttcgggtac gacgcggggg
      781 tcggtggtgt aaacgccgtc tacgtcggta tagatttggc attcgtccgc tttgagggct
      841 gcggcaagcg caacggcgga agtgtcggaa ccgccgcgtc ctagcgtgga aatattgcct
      901 tcactgctga tgccttggaa gccggcaacg atgacgactt tgccggcggc gaggtcggcg
      961 cgcatttttt cgtcatcaat gctttctatg cgggctttgg tgtgggcggt atcggttttg
     1021 agggagacct gccagcctgt gtagcttttg gcatccacgc cgatatcctt caatgccatt
     1081 gccaaaaggc cgatggttac ttgttcgccg gtagctaaga cgacgtccag ctcgcgcgga
     1141 tcgggatgct cctgcatttc gtgcgccaac gcaaccagac ggttggtttc gccgctcatg
     1201 gcggatacga cgacgacgat atcgtgtcct tcggcgcggg ctttagcgac acgtttggcc
     1261 acgtttttaa tgcgctcggg cgagcctacg gatgtgccgc cgtatttatg tacgattaac
     1321 gccatgtttc atgctttctt gtgtgggatt ggtcgggcag catggtttgc tggaaaagcg
     1381 gctattatta ctatttttta catgaagttc aagaacggac tgcgctttcc cgcctgccgt
     1441 ttgacagcgg tcagcgaaaa acctgttctt tcagattttt gacaaaatgc cgtctgaacg
     1501 gttttcagac ggcatacgga cagtaattaa gcaacggaca acgcattttg ctgataacgc
     1561 agcagttcgc ctatgccttt ttgcgccagt gcaaccagtt tgcccaactc gtccaaactg
     1621 aatggcgcgc cttccgccgt tccctgtatt tcgatgattt tccccgatgc ggtcatgacg
     1681 atgttcacat cactgtcgca gccggagtct tcgggataat ccaaatccaa aagcggcacg
     1741 ccgttcacta cgcccgctga tacggcggca acagcttcaa ggatggggtt ttcactcaaa
     1801 atgccgtctg aaaccagttt gccgacggcg atttgcagcg cgacaaacgc accggtaatc
     1861 gaagccgtac gcgtgccgcc gtccgcctga atcacatcgc aatcaatcag gatttgtcgt
     1921 tcgccgagtt tttccatatc cacgaccgcg cgcagcgaac gcccgatcaa gcgttggatt
     1981 tcttgtgtgc gtcccgactg tttgcccgcc gaagcttcgc ggcgcatacg caaagcggtt
     2041 gaggcaggca gcataccgta ttccgccgtt acccagcctt ggtttttgcc gtgcagaaac
     2101 ggtggggcat tttcgtccac ggaagcggta caaatcactt tggtattacc gcattcaata
     2161 aggcacgaac cgtccgtatg cggcaggaaa tgaggggtga ttttgatatc gcgcaggctg
     2221 tcggcggcgc gtgaggtgcg gatataatca ggcatactgc cctcccatta aaaacagata
     2281 aattaaaaag ccttaaatat gaaaaatcac atttaaggcc ttcaaactga aaatttctac
     2341 gcctcttcgg ctttgctacg gataatcaaa agcggcaggt ggctttggcg cattaccgtt
     2401 tcggcgaaac tgcccatcaa aaggtgcatc agcccggtgc gcccgtgcgt gcccaacacc
     2461 agcaggccgg caccgttttc atcggcataa tcgaccaaat cctgcgccat ttcgcgcgca
     2521 cccttattgg caaccagcag gtgtttgacg gtatttccca cacccagttc ccgggcggtg
     2581 cgctcggcgg catccaaaac ctcgttgcct tgcgcgacgg cggcggcctc gtaactttcg
     2641 tgttgcaaaa attcgggggc gagcgccata tattcggcag ggttggcaac gtgtaccaaa
     2701 gtcagacgcg caccgttgac cccggcaagc tcggcggcgt gtttcagggc attgatggac
     2761 gtttcactgc cgtcaacggc aacgaccaga tgtttgtaca tatcgtattc tccttttgca
     2821 ccgtctcacg gtgccgtctc ttcaggtttg cgcaaaggca gtctgcgccg tttcattata
     2881 gacccgccgt cgggctttat acaacagccg aacagcctga ctgctttcca gtataatatg
     2941 ccgctttcgt gcagtcaggc attttttgcc ggctttcgtt cactttttga tttgacgcaa
     3001 tcttgcagga ttcgaccatg tccgacaacg ctttgacctc ttcgcgacgc ttcggcggca
     3061 tcgccagact ctacggagac tctgccttga cgcacttttc acaggcacac gtctgcgtgg
     3121 tcggcgtggg cggggtcggc tcgtgggcgg tcgaggcttt ggcgcgcacg ggcatcgggc
     3181 gtttgacttt gattgatttg gacaacgttg ccgaatcgaa tgccaaccgc cagctgcacg
     3241 ccctgaccgg cgacttcggc aaagcaaaag ttaccgcctt gcgcgaacgt attacacaaa
     3301 tcaatccgca atgcgaagta tttgaaattg aagatttcgt taccgaagac aatttgccgg
     3361 aatacttcgg aaaaggtttt gatttcgtca tcgacgcgat cgaccaagtg cgcgtcaaag
     3421 cggcaatggc ggcttatttt gtggaacgca aacaaccgtt tgtcctcagc ggcggcgcgg
     3481 gcggacagaa aaatcccgcc ctgattcaaa ccgccgattt aagccgcgta acccacgacc
     3541 cgctgcttgc caacctgcgc tacaccttgc ggaaacgcta cggcttcagc cgcgatacta
     3601 aggaaaaaat gcgcgtgccg tgcgtgtatt cgaccgaaaa tatcatgccg ccgcaatcgg
     3661 gcgcagcgtg ttctgccgat gccgcgccgc agggcttgtc atgcgccggc tatggcgcaa
     3721 gtatgctcgt taccgcttcg ttcggactgt attgcgcaca ggcggcggtg gaacacatcg
     3781 cgggcaaaaa ataagcaatg ccgtctgaaa caggattcag acggcatttg aacaaacgcg
     3841 gcaatcaagc tgtcggttca gaaaaaccaa catcggcacg cctaaaaccg tattcacaat
     3901 ctactccttt tcaaaatatt tgcatttaaa agccgttata atgccgtccg aacatctgcc
     3961 cgaccacatt atgcgtgaat gccggcagat tgttttcttt tgtaaactta tattaaaatt
     4021 cacttaccaa ttcacgccat gccgcccatc cctgccctat ccgcaccatc cgagcacact
     4081 gtcgcatggg tattcggcca acccgttacc gatttgccgc aggatttgtt tatcccgccc
     4141 gacgcgttga aaatcgtgtt gggcagcttc caaggccctt tggatctgct gctctacctt
     4201 atccgcaagc agaacatcga tgttctcgat attccgatgg tggaaattac cgggcagtat
     4261 ctgcactata ttgcccaaat ggaagcctat cagtttgatt tggcggcgga atatcttttg
     4321 atggcggcaa tgctgattga aatcaaatcg cgcctgctgc tgccgcgtac cgaagccgtc
     4381 gaagacgaag aggccgaccc gcgtgccgag ttggtgcgcc gtctgcttgc ctacgagcaa
     4441 atgaaactgg cggcgcaggg tttggacgcg ctgccgcgtg cgggacggga tttcgcgtgg
     4501 gcttacctgc cgctggaaat tgcagccgag acgaagctgc ccgaggttta catcgccgat
     4561 ttgatgcagg catggttggg cattctttct cgggcaaaac atacgcgcag ccacgaagta
     4621 atccaagaaa ccctttccgt gcgcgcgcaa atgacggcaa tcctgcgccg tttgaacgaa
     4681 cacgggatat gcaggtttca cgacctgttc aatcccgaac agggcgcggc ttacgtgatc
     4741 gtcaacttca tcgccctgtt ggagcttgcc aaagaaggat tggtcggaat cgtacaggaa
     4801 gacggtttcg gagaaatccg aatcagcctc aatcatgagg gggcgcattc agacggcatt
     4861 ttcggcacac ggggcgggcg cgatgtgttc taatacgccc caagccgcca ccagaaatcg
     4921 ggagacacgc catatgaccg gcatcataca ttcgctgctc gacactgacc tctacaaatt
     4981 cactatgctg caagtagtcc tgcaccagtt tccgcagacg cacagccttt acgaattccg
     5041 ctgccgcaac gtctcgaccg tctatccgct tgccgacatc agggaagact tggaagccga
     5101 actcgacgcg ctctgccggc tgcgcttcac ccacgacgaa ctcggctatc tgcgctccct
     5161 gcgtttcatc aaaagcgact ttgtcgatta tctcgaactc ttccagctcc aacgccgctt
     5221 tgtcgaagtc ggcacagacg ataaaggccg tctgaacatc cgtatcgaag gcccgatgat
     5281 acaggcgatg ttttttgaaa tcttcatcct cgccattgtc aacgaacttt acttccgccg
     5341 gctggaaacc cctgccgtta tcgaagaagg cgaacgccgg cttcaagcca aagccgcgcg
     5401 cctgaaagaa atcgccgccg cacagaatcc ggacgaaccg cccttcctga tttccgactt
     5461 cggcacgcgc cgccgctaca agctcgcgtg gcaggaacac gtcatccgca ccctgctcga
     5521 agccgcccca agcatcgtgc gcggcacaag caacgtttac ctcgccaaaa aactcggcat
     5581 cacccccatc ggcaccatgg cgcacgaatt cctccaagcc ttccaagccc tcgacgtgcg
     5641 cctgcggaat ttccaaaagg ccgcgctcga aagctgggtg cacgaatacc ggggcgattt
     5701 gggcgttgcc ctgaccgacg tggtcggtat ggatgccttc ctgcgcgatt tcgacctcta
     5761 tttcgccaaa ctcttcgacg ggctgcgcca cgacagcggc gacccttacg tttggggcga
     5821 caaagcctac gcccactatc aaaagctcaa aatcgacagc cgcaccaaaa tgctgacctt
     5881 ctccgacggg ctggacatcg aacgctcttg ggcattgcac caatatttca aaggccgctt
     5941 caaaaccggc ttcggcatcg gcaccaacct caccaacgat atggggcata cgcccttgaa
     6001 tatcgtgttg aaactggtcg aatgcaacgg gcagtccgtc gccaagctct ccgactcgcc
     6061 gggcaaaacc atgaccaaca acagcacctt cctcgcctac ctgcgccaag tgttcggcat
     6121 acccgaacct agaacgccgt aaaccggcag aaaaagcgta caattcctgt ttccgccgca
     6181 taaaatcttt taaaataccg cctgatttga atttaaccga aagaccgaac ttcatgaacc
     6241 tacatcaaac cgtcgaacac gaagccgccg ccgcctttgc cgccgcaggc atcgccggca
     6301 gccccgttgt cttgcagccg accaaaaatg ccgaacacgg cgatttccaa atcaacggcg
     6361 tgatgggtgc ggcgaaaaaa gccaaacaaa atccgcgcga actggcgcaa aaggtcgccg
     6421 acgcattggc gggcaacgcc gtcatcgaaa gcgcggaagt ggccggaccc ggctttatca
     6481 acctgcgcct gcgccacgaa tttctcgctc aaaacattca tgcggctttg aacgacgcgc
     6541 gtttcggcgt agcgaaaact gcccaaccgc aaaccgtcgt tatcgactat tcttcgccta
     6601 atctggcgaa ggaaatgcac gtcggccatc tgcgctccag catcattggc gacagcattt
     6661 cacgcgtgtt ggaatttacg ggcaacaccg tcatccgcca aaaccatgtc ggcgactggg
     6721 gtacgcagtt cggcatgttg gttgcttatt tggtcgagca gcagaaagac aatgccgcgt
     6781 tcgagctggc ggatttggag cagttttacc gcgccgccaa agtgcgcttt gacgaagacc
     6841 ctgcctttgc cgacaccgca cgcgaatacg ttgtgaagct gcaaggcggc gatgaaaccg
     6901 tgttggcgtt gtggaaacag tttgtcgata tttcgctctc gcacgcccaa gccgtttacg
     6961 acacgctggg cttgaagctg cgccccgaag atgtggcggg cgaatcgaaa tacaacgacg
     7021 atttgcagcc cgtagccgat gatttggttc aaaaaggcct ggcggttgag gacgacggcg
     7081 cgaaagtcgt gttcttggac gagttcaaaa acaaagaggg cgaacctgcc gcatttatcg
     7141 tgcaaaaaca aggcggcggc ttcctttatg cttccaccga tttggcgtgc ctgcgctacc
     7201 gcataggccg tctgaaagcc ggccgcctgc tgtacgtcgt cgaccaccgc caagccctgc
     7261 acttcgagca actctttacc acttcccgca aagcaggcta tctgcctgaa aatgcaaaag
     7321 ccgagtttat cggcttcggc accatgatgg gcaaagacgg caaaccgttc aaaacgcgca
     7381 gcggcgacac cgtaaaactg gttgacctgc tgaccgaagc cgtcgagcgc gccaccgctt
     7441 tggtgaaaga aaaaaatccc gaattgggcg cggatgaagc cgccaaaatc ggcaagaccg
     7501 tcggcatcgg cgcagtcaaa tacgccgatt taagcaaaaa ccgcaccagc gattatgtgt
     7561 tcgactggga cgccatgctc tcatttgaag gcaacaccgc cccctacctg caatacgcct
     7621 acacccgcgt gcaaagcgtg ttccgcaaag ccggagaatg ggacgcaacc gcaccgaccg
     7681 ttttgaccga accactggaa aaacagcttg ccgccgagct tctgaaattt gaaaacgtgc
     7741 tgcaaagcgt ggcggacacc gcgtatccgc actacctcgc cgcctacctt taccaagccg
     7801 ccaccctgtt cagccgcttc tacgaagctt gcccgatttt gaaagccgaa ggcgcaagcc
     7861 gcaacagccg cctgcaactg gcaaaactca ccggcaacac gctgaaacaa ggcttggatt
     7921 tgctgggcat cgatgtgttg gacgtaatgt aaaaccgcac cgcccgattg cggacaacag
     7981 ccctgcaatc aaccggcgga ccatcctcat ccgaatctga aaaagcggcg cgatacaccg
     8041 tatccgccgc ccctcccaaa atgcgaaaca gacaaacgcc aagcaagcaa gcaagcaagc
     8101 aagcaagcaa aaaattatac ccccccttcc tgccgacgca cgcactttcc gcgcggcgta
     8161 ttcccctttt cccgcccctc aaacccgcct tttcttcagg cagggtttca gcccgcctcc
     8221 tttccctgtt ttccttccct caacacgcgt gctcgccccc tgccgcaccg tgccgcactt
     8281 tcgcgcccgg acggcatcgt tccgccatcc ggttctctgt ttcaaatacc cctgtttcag
     8341 aaagaaatgc agatgtttca acacacagga cgacatataa agtaccgccc tatgtgtcgt
     8401 cctgatttgg aaggggttgc gccccttcct aaatatagtc tgatcctgcc gccccgaagg
     8461 acagatgtcc gagtggcggg gt
//
LOCUS       DAWTHD010000093         8312 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWTHD010000093 DAWTHD010000000
VERSION     DAWTHD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.093"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(1..370)
                     /locus_tag="WM704_001712"
                     /pseudo
     CDS             complement(1..370)
                     /locus_tag="WM704_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(370..1671)
                     /gene="nuoF"
                     /locus_tag="WM704_001713"
     CDS             complement(370..1671)
                     /gene="nuoF"
                     /locus_tag="WM704_001713"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFA9341081.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            complement(2061..2534)
                     /gene="nuoE"
                     /locus_tag="WM704_001714"
     CDS             complement(2061..2534)
                     /gene="nuoE"
                     /locus_tag="WM704_001714"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFA9341082.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(2534..3790)
                     /gene="nuoD"
                     /locus_tag="WM704_001715"
     CDS             complement(2534..3790)
                     /gene="nuoD"
                     /locus_tag="WM704_001715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFA9341083.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(3780..4373)
                     /locus_tag="WM704_001716"
     CDS             complement(3780..4373)
                     /locus_tag="WM704_001716"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFA9341084.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(4386..4868)
                     /locus_tag="WM704_001717"
     CDS             complement(4386..4868)
                     /locus_tag="WM704_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HFA9341085.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(4859..5215)
                     /locus_tag="WM704_001718"
     CDS             complement(4859..5215)
                     /locus_tag="WM704_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFA9341086.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            complement(5676..7127)
                     /locus_tag="WM704_001719"
     CDS             complement(5676..7127)
                     /locus_tag="WM704_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFA9341087.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(7150..7770)
                     /locus_tag="WM704_001720"
     CDS             complement(7150..7770)
                     /locus_tag="WM704_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFA9341088.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
     gene            complement(7969..8312)
                     /locus_tag="WM704_001721"
                     /pseudo
     CDS             complement(7969..8312)
                     /locus_tag="WM704_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2177 a         2071 c         2068 g         1996 t
ORIGIN      
        1 ctcattgtct tcatctgtag gctcaaactt ttcacaccaa ttaattatat ttacatcgcc
       61 atgcaatatc ctactgcatc gcaactacca ccccgtgtcc ttgacgtgcc aaaataaaat
      121 cacaattatg cgaataaagt ttgtataaat ttctcctaac ctcgtattca ttatcaccag
      181 atgaataaaa accaatcctc tgaccttcat gatcaaatac tgcaacaaaa tctaagccgt
      241 ttccatatct ttcaaaaaag actagaaatt tagaaaattt ccgacaaatc tgattaaaaa
      301 gcgtattgag tgtcgtactc ttacctttgt tggctgcacc gtatagaata aaaattttag
      361 cttccaccat taccaccctc cccacttatg ctctttcatc ggcccgccgt gttcgatgta
      421 atgcgcaaac tcatcacgga aatgcttggt aaagctgcgg acagggaaga cggcggcatc
      481 ggcaagggcg cagatggtgc ggcctgccat ttggttgccg acagaatcca gcaaatccaa
      541 gtcttccatg cggcctttgc cttctacgat gcggcggacg atgcggtaaa gccagccggt
      601 accttcgcgg cagggggtgc attggccgca ggattcgtcg tagtagaaat agcttaagcg
      661 ttcgagggct ttgaccatgc acacgtcttc gtccatcacg ataatcgcgc cggaaccgag
      721 catcgagccg gctttggaaa tcgagtcata gtccatattg gtctgcatca taatgtcggc
      781 aggcaatacg ggggcggacg aaccgccggg aataacggct ttgagttttt taccgccgcg
      841 cataccgccc gccattttca agacttcggc aaacggcgtg cccaacggca cttcatagtt
      901 gcccggacgc tcgacatggc cggaaataca aaataatttg gtaccgcctg cattcggaat
      961 acctttatcg gcaaacgcct gtccgccgtc acggataatg aatggaacgg aggagaacgt
     1021 ttcggtattg ttgatggtgg tcggtttgcc gtacaggccg aacgaggcgg ggaacggcgg
     1081 tttgaagcgc ggctggcctt ttttgccttc caacgattca agcaatgcgg tttcttcgcc
     1141 gcaaatatat gcgccgtagc cgtggtgggc gaagagttcg aattcaaaat ccgaacccaa
     1201 aatattttta cccaaaaagc ctgcggcacg cgcctgctcc aaagcggctt caaagcgttg
     1261 ataaccttcg aaaatttcgc cgtggatata gttgtagccg gctttcgcgc ccatcgcgta
     1321 accggcaatg atcatgcctt caatcagggc atgaggattg aacatgatga tgtcgcggtc
     1381 tttaaatgta cccggctcgc cttcgtcggt gttgcaaacc acatattttt cgcccgggaa
     1441 agaacggggc ataaagctcc atttcaaacc ggtcgggaag cccgcaccgc cgcgcccgcg
     1501 caagccggag gttttgactt cgtcaatcac atcggtttgc gagatgtttt cggacaggat
     1561 tttacgcagg gcggtatagc cgccgcgttt gacgtattcg tccaatgtcc agcaatcggg
     1621 attggcggta tccacttggt caaaaatcac gcctgattgg taaatagcca tttttggtgt
     1681 gcctgtttgt tttcgtatcg gttgcggccg ctgtttcaga cgaccttaaa atgtctttgt
     1741 gtatcggctt gtaacgtctt ctgaaataaa atctagttta tcaaatcgat attttcagac
     1801 gaccttcaat cgtttttgtt ggatctcgac cacttgcttg tctgccgtca ttcccgcgca
     1861 ggcgggaatc catcctcaat ggtaagcaat gtcttattaa attcagaaac cgaatcttac
     1921 cggtggattc ccgcctgcgc gggaatgacg gcatttcagt atttcagtag ggcggattct
     1981 taaatccgac attttgcctt tttacccact ctgtcggata caagtatccg acctacgttt
     2041 aaatcgtcgt ttcagacgac ctactccaac tccgccagtt tcttctcaat cgcctcttcg
     2101 gtcataaagc tgcacatgct gtggttgttg accagcataa cgggggcatc gccgcacgcg
     2161 cccatgcatt cgccttcgac aagggtaaat ttgccgtcgg gcgtggtttc gccgtagccg
     2221 ataccgagtt tttgtttgag gtattcgccg gtcgccatac cgccgcgcag agcgcagggc
     2281 aggttggtac aaacggtcag tttgtatttg ccgacaggct caaggtcgta catattgtag
     2341 aaagtggcga cttcatatgc ctgcgccggc gtgatgccga tgtagtcggc aacaaaagcg
     2401 atggtttcgg gggcgagcca gcctttttcg gtttgggcga tacgcaacgc gcccatgatg
     2461 gcggaacggc gttggtcggc gggatatttc gccaactcaa tgtcgatttg ttttaaggat
     2521 tttgcggata acattatcgg tcaacctccc cgaatacgat gtcctgcgta ccgatgatgg
     2581 caacgacgtc ggcgagcata tggccttttg ccatttcgtc catgccttgc aggtgggcga
     2641 agccgggtgc gcggattttc aggcggtagg gtttgtttgc gccgtctgaa atgatgtaaa
     2701 cgccgaactc gcctttcggg tgttcgacag cggtgtaggt ctcgccctcg ggaacgtgca
     2761 taccctcggt aaagagtttg aaatggtgaa tcaggtcttc catacctgtt ttcatttcgg
     2821 tacgtttggg cggagcgaat ttgtggtttg cggtaatgac cgggccggga ttgacgcgca
     2881 accagtcggc gcattgtttg atgatgcgta cggattggcg catttcttcc atacggcaga
     2941 ggtaacggtc gtagcagtct ccgttgacgc cgacggggat gtcgaaatcc attttgtcgt
     3001 acacttcgta gggctgtgtc ttacgcacgt cccattccac gccggaaccg cgaagcatca
     3061 caccggtaaa gcctttttgc atggcgcgct cgggagagac aacgccgatg ccgacggtac
     3121 gctgtttcca gatgcggttg tcggtcagga gggtttcgag tgtgtcgata tttttgggga
     3181 agcgttcgca gaaggcatcg ataaagtcaa gcatcgtgcc ttcgcgggat tcgttgagct
     3241 gcttcaatac tttggcattg cggaatttgc tgctctcgta tttgggcata aagccgggta
     3301 ggtcgcggta aacgccgccg ggacggaagt aggcggcgtg catacgtgcg ccggacacgg
     3361 cttcgtacaa gtccatcagc tcttcgcggt cgcggaaggc gtagaggatg gcggtcatcg
     3421 cgccgatgtc gaaggcgtgc gaaccgatgc ccatcaagtg attgaggatg cgcgttactt
     3481 cggcgaacat cacgcggatg tattgggcgc ggataggcac atcgataccg gcaagttttt
     3541 ctaccgccaa gcaatacgcc tgctcgttga ccatcatgga aacgtagtcc aaacggtcca
     3601 tatagggcag ggcttgcaga taggttttgg tttccgccag tttttcggtg ccccggtgca
     3661 ggaggccgat atgcggatcg gcacggacga tttgttcgcc gtccagctcc aaaatcatac
     3721 gcaatacgcc atgcgccgca gggtgttgcg ggccgaagtt gatggtgtag tttcttaatt
     3781 tattggccac cgtagttctc ctcacggacg atacgcggtg tgatttcgcg cggctcaatg
     3841 gtaacaggtt ggtaaatcac gcgtttttgc tcttcgtcat aacgcatttc cacatagccg
     3901 gaaatcggga agtctttgcg gaacggatgt ccgacaaagc cgtaatcggt caggatgcgg
     3961 cgcaagtcgg gatggttgtt gaacatgatg ccgtacaaat cgaaggcttc gcgctcgtac
     4021 caatccgcgc tgttgtaaat atcggctacg gattcgacta cagggaagtc gtcgtctgaa
     4081 acccagacgc gtacgcggat gcgttgattg tttttaacgg aaagcaactg actgacaacg
     4141 gcaaagcgtt tgccctgcca tgcttcgttt ttgtaagtgc tgtaatcgac gccgcacaag
     4201 tcaaccagaa gctcgaaatg caaatcttca tggtcgtgca atgcggtcat gactgaaata
     4261 tagtgctcgg gcagacactc gacggtaatc tcgcccaaag ctgaaatgac tttgcctgcc
     4321 tgatcgccaa gcacgccgac gacggtctcg tataagtttt gaatgcttgc catatcgtcc
     4381 tctccttact cgtcacgcgc gatggtggaa gtgcgcttga ttttttgttg gagctgaatc
     4441 aggccgtaaa tcagagcttc cgcagtcggc ggacaacccg gcacataaac gtccaccggc
     4501 acgacacggt cggcaccgcg cacaacggaa taagaatagt gataatagcc gccgccgttg
     4561 gcacacgaac ccatagacaa tacccagcgc ggctcggcga gctggtcgta cacgcgacgc
     4621 agggctggcg ccattttatt ggtcagcgta cctgccacaa tcatcaggtc ggcctgacgt
     4681 ggggacggac ggaaaatgat accgaaacgg tcaaggtcat aacgcgccat acccgcatgc
     4741 atcatttcca cggcgcagca ggccaatccg aaagtaaccg gccacaatga accggtacgc
     4801 atatagttca acaccgtatc cgcgctggtg gtgatgaaac cttttttcaa aacgccttct
     4861 attcccattc cagcgcacct tttttccatt cgtaaacaaa gcctaccgtc aggacgacga
     4921 taaacaccag catagaccaa aagccgtatg cgcccaaatc tttgaacacg actgcccacg
     4981 gcagcataaa cgcaacctcc aaatcgaaga ggatgaacag gatggcgacg aggtaatagc
     5041 gcacgtcgaa cttcatcctt gcgttttcaa aggcttcaaa accgcattcg taaggcgcgt
     5101 ctttttcggc atagtggcgt ttcgggccca aaatcgtgcc gagcaggata aacagtacgc
     5161 cggccgcgag gccgacgagg ataaagacaa agacgggaaa ataagcggac aacatggtta
     5221 cacccaaatc cgttaacaaa atttctacaa taatttcgta ttttagcgaa tttcaaaaac
     5281 cattaaaagg taaatatcgg caaaacgccc aaaaaaaccc aataaataca atcatgttat
     5341 gataacgatt cttatttgat tttataaggt catataaatt tttacaccgt aattcgtggt
     5401 atagcggatt aacaaaaacc ggtacggcgt tgccccgcct tagctcaagg ggaacgattc
     5461 cctaaggtgc ccaagcacca ggcgaaccgg ttccgtacta tttgtactgc ctgcggcccg
     5521 ccgccttgtc ctgatttttg ttaatccgct atatatagga ataagaattc gggacaaaat
     5581 gtttaaacca ttttgtcccg actgctgtga tgcggttttt ttgaactagc aggacgttac
     5641 ctaaaacctg cgttgcctga aaccctttaa gacggttaaa tccctctgcc gtatttgtat
     5701 tctacaatca tattatcgtc agtaatgact tccgccccag ccgaaggttc cgtcatccga
     5761 atcagcatac gagagacaac cttttgtgct gcagcatcca cggtgctgct gtcaaatacg
     5821 tgcctgccgc tttccggcca aatcaaccgg gaaaggcgtt gcttgagcag ttctttatta
     5881 gggaaaacta ccggggttgc cgagccgact accatatgcc cgtagcggta tgcataggga
     5941 atactgtgta cggcggtagc aaaagcatgc gggctgtgcg tggtattaaa cattacaata
     6001 ccatccgggg taaggtggct ttgcacctgt tttaaaaatt ccgcactcaa caggttagtg
     6061 gaataggcac gccagtacca agtcgaattc atcaaaatca ggtcgaattt ttcatcagga
     6121 tggcgacgca gccatttcct accgtcatcc aatacaattt caacacgttt gtcctgcaaa
     6181 agcggtgcga tttgcggctc gtccgcgata aggctacggt atgccggatt gatttccgca
     6241 acgatcatcg actgcatttc cggaatggca gacaagacgc gcgcccacga acctgtactc
     6301 aatccaacga cgaaaatgcg gcgtatgccg gacttcaggg agggtagcag ataggcacgt
     6361 tcgatgccgt tgacactatt gaatatatcg gtattgtatg cgccgtcgta tacattcgcc
     6421 ccataaacaa ccttatcacc atctctatgg taaaccgcaa caatgccgtg tttgttttca
     6481 atcaacctat ccggacggcc agcaatattt tgaaagacag aatccggcag taggaacatg
     6541 aggatgccga acattaggga aactgctacc gacactgcat tcagtcggag acttttttgg
     6601 aacagtgtac aaaacaaagg gacagcagca gaaatcaaac agatgagcag gtaaatctgt
     6661 tgggtggaca agaaatcaag tatcacaaag ccgataagga ccggacccaa tgcactgccg
     6721 gcaacgttgg cgaaataaac attggaaacc tgtcgtccgg atttgttgcc atccgtaccc
     6781 acatggtgta caagtgggaa aatcaacccc ctgacgacgg cagacagggt aatgaagata
     6841 ccggcgtggt ggacgaagcc ggaaaaaccc gtcaacaacc acgcagcacc caaaatcaaa
     6901 aaatcggcaa tacccgccca caagaagcac tgcccgataa agggaatatc aacaaagcgg
     6961 ctgcggcaaa tccgtttgcc aaaatacgcg ccgacggcga taccggtcag aaaacaggca
     7021 agagtaaatg aaaatgcctg aggcacggac tgtgctgcga acgaaaacat ccttacccac
     7081 aagacttcta tacccaagct caataatccg ctaaggaaag aaagcatata aatcaaacta
     7141 gtattcggtt tagtgttcac catatccatc ctttctgtaa cgcagccata ctgaagcagc
     7201 aatcagaagg ttaaggcagg ctgtcagcgc aatggtttgg gagagggtaa aaaagacgta
     7261 gaaaaattcg gcggcggcaa gcgatccgag tgccgcaccc aaagtgttga aaaaatataa
     7321 ggtaccgata gactcgccaa cattatgtat tttccggtta aaaaaacagg tcagcaaggg
     7381 caaggtcgcg cccatcataa aggtaggaag cagcaataag aggaaattgg cagcagcgat
     7441 gatgggcaaa tcagcctcaa ctaaaagatg ccccaagccg gaaatcagac ccttgcttac
     7501 caaaccgaac agaccgatgg atacttcagc gatgcaaaac agggggatga tacttgaagg
     7561 aaaacggtca gcaatgcgtc cgccgaaata cgcacctaca cccaagccga ccataaatac
     7621 agaaataatg acagtaatcg aactcaaatc gatacctatg tggctgaata gaagcctctg
     7681 ccagctgacc tggtaaatca gggcgcagaa gccagaggcg aaaaacacca gcgacaatcc
     7741 tttcagggtt ttacttgcgg tcgaattcat attttctatt ttcaaatagg cggaactata
     7801 aatgaaatgt attaatgtgt aaacatttgt attagccgta ggaaatcacc gcattgctca
     7861 aactgcaggc cgggaaaata aactcgtaag cccgcaaacg gcgtatcact gttcaaactg
     7921 ctgtgcggct tcatgacgtt ataaaaatta aggggctgtc ctggacaact aggataaact
     7981 cgattttgct aattgtttta aaatggaaca agaactttta tctcactgtt gttaaaacgc
     8041 cgttcgcact cctttaaata cggctcaaaa tgctctttgg gaatgccgtc aaacttgcgt
     8101 aaatgacgtt ttgcccggtt ccaaaagttc ccaattccat taatatggtt ttgtcgttcg
     8161 gcaaaatgtg tgctgtgatt gatacggaaa tggctaaatt cgcccgcatc caatacatca
     8221 tagcgacgat aacaatccgt ataaacaatg ctgtcaggct ctgttgctgt tcttgcagtt
     8281 acgcctgcga caaacagttc aatgagttta tt
//
LOCUS       DAWTHD010000094         8214 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWTHD010000094 DAWTHD010000000
VERSION     DAWTHD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.094"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(14..430)
                     /gene="dksA"
                     /locus_tag="WM704_001722"
     CDS             complement(14..430)
                     /gene="dksA"
                     /locus_tag="WM704_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFA9341089.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(579..1370)
                     /locus_tag="WM704_001723"
     CDS             complement(579..1370)
                     /locus_tag="WM704_001723"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFA9341090.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLGKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTLSRYLGG
                     SRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1419..1826)
                     /locus_tag="WM704_001724"
     CDS             complement(1419..1826)
                     /locus_tag="WM704_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFA9341091.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1826..2512)
                     /locus_tag="WM704_001725"
     CDS             complement(1826..2512)
                     /locus_tag="WM704_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFA9341092.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2632..3675
                     /locus_tag="WM704_001726"
     CDS             2632..3675
                     /locus_tag="WM704_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFA9341093.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3839..5065
                     /locus_tag="WM704_001727"
     CDS             3839..5065
                     /locus_tag="WM704_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFA9341094.1"
                     /translation="MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5182..7332
                     /gene="yccS"
                     /locus_tag="WM704_001728"
     CDS             5182..7332
                     /gene="yccS"
                     /locus_tag="WM704_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HFA9341095.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
     gene            complement(7812..8096)
                     /locus_tag="WM704_001729"
     CDS             complement(7812..8096)
                     /locus_tag="WM704_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9341096.1"
                     /translation="MICTVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
BASE COUNT         2007 a         2706 c         1788 g         1713 t
ORIGIN      
        1 ttgtttgcct ccatcagtcg gcaaactgtt tcttcatcct ctctcggcgt tcttgggcct
       61 caacggataa agtggctgtc gggcgtgcca gcagccgctt cagaccgata ggttctcccg
      121 tatcggcaca gaatccatag tccccttcat caatattgcg gatggtcgcc tgtattttac
      181 tgagaagttt tcgttcccga tcccgggtac ggagttccaa tgcgtactct tcttcctgtg
      241 tggcacggtc ggcaggatcg ggggctgatt cgtgttcttg gagatgccct gttgtaacgg
      301 aggcgttttc gatgagttcg tcttgcattt ttaccagcaa ttcgcggaaa aaagccaaat
      361 ggtcgtcatt catataatcg tcttccggcc caatccaatt caaaatatct tgttctgtca
      421 gctttaccat aatgtatgcc ctctcggttc accgcaaaaa aacaaagcac tacaaagtgg
      481 ctttattcct ttgattttta taaggcttat tgcctaaggc gtttttgaaa acgcgcatca
      541 taacacgttt tggattggtt ttttttattt tctttacatt attgatattg ccgttccatt
      601 tcctgcgaac ggcacacaca ggcacaaacg ccctcactta tggcttcggc gacacgatgc
      661 cgtctgaagg cttccacggc ttcgtgggtt gtcccgcctt ttgacgttac atttttttga
      721 agcttctcga aatcttcacc cgtctgctcg gcaagggcaa ccgctccttt aaacgttgcc
      781 agactgagcg cgcgtgcttc tgccatatca aacccttgtc ggatggcggc attttgcaat
      841 gcgtccagca gataaaacac ataagccggc ccgctgccgc tgatgccggt aatgctgtgc
      901 atttgcgctt catcttccaa ccaaacggtc aaaccgactg atttcatgat tcgatcggca
      961 atcctgcggt ctgtttccga tacttccgct tcggcataca taccggatac gcccagcccg
     1021 atttttccgg gtgtattcgg cataacccgg acaatgcggc ggcttccccc gaggtaacgg
     1081 ctgagcgtac cgaccgacaa tccggctgcg acagaaagca ccaatgcacc gttggtgcgg
     1141 atatttttgc acgcggcttc catatcctgc ggtttgacgg caaggattaa aacatcgtcg
     1201 gaatgaagct ccggcagggt tgccgaagtt tcgaccccca actcttttcc caaacgttcg
     1261 cgcttttccg caccacggtt ggctatatgg atgcggtatc ccccttgttt gaccaatccg
     1321 cccgcaacgg cagccgccat atttccgccg ccgagaaaat aaacattcat tatttctacc
     1381 ctattcaaaa ttaccttact aaattcattc ccaatttttt accatcggac gcgctcggac
     1441 aggccgttgc gccgcaaccc ctgcttttcc aaacagtcga aaccttcctg tttgaacctc
     1501 tcaaccaaag gtttgccgtc acgcgtacgc caatccaacc cgcaggcttc aaacttccct
     1561 attttcctat aaaagtaaga tttttgcgcg gattcatatt cgtttgccct gacggaacga
     1621 ttgccgtctg aaagcggtat ctcgtagtcc tctaaagaca gcggactctc gccgccaata
     1681 tcccaaaaat cgatggcggc aggttttctc ggcttacacc agccggacaa ggacgaacac
     1741 tcataccacc ccgccagccg gcagcctgca accgtaaatg caaacaacgc agccgttgcc
     1801 gtatatttta ttacccgaat ttccatcagc cccttttccc gaaaatcgcg ctgccgatac
     1861 ggacgtgtgt cgcaccgcac tcaatggcgg caggcatatc gtccgacatc cccatagaca
     1921 gcacgtccgc cttaacgcca gccgcattga ggtcggcaag cagcttttgc atggtctgaa
     1981 actgagtttt caactccgtt tcactgctgt tggctttggc aacgcacatc agtccgcgca
     2041 cgacaaggtt gggcagcttc gccacttcca cggcaagcgc gaccgcttct tcgggcgcga
     2101 caccgtgttt tgccgcctcg cccgcaatgt ttacctcgac acacacctgc aagggcggca
     2161 ttaaggagcg acgctgcctg ctcagccgga cggcggtctt cagacggcat acggtatgca
     2221 cccaatgcgc gcgttcggcg acaaatttgg ttttgttgga ctgcacatct ccgatgacgt
     2281 gccacacgat gtcggtcaaa tccgccaatt cctccgtttt gccgtaccac tcctgaatat
     2341 agttctcgcc gaaatcacgc tgtccggcag cgtaaacttc gcggatgccg tctgaaggga
     2401 aggtcttgct gacggcaatc aggctgacgg aatgcggctc cctgcccgcc tgcagaacca
     2461 attttccgat acggtcggac acctcacgat aacgttcttg caacaccgtc atagatcatc
     2521 ccctaattaa aaatgattaa acagttgaaa cccctccagt cagggcggta taatcaaggt
     2581 tgttagaatc attccaacca atcgaaacat tatactaaac agagccgcat tatgcagatt
     2641 accgacttac tcgccttcgg cgctaaaaac aaagcatccg accttcacct gagttcgggc
     2701 atatccccta tgattcgggt tcacggcgac atgcggcgca tcaaccttcc cgaaatgagc
     2761 gcggaagagg tcggcaatat ggtaacttcg gtgatgaacg accaccagcg gaaaatctac
     2821 cagcaaaact tggaagtcga cttctcgttc gaactgccca acgtcgcccg attccgcgtc
     2881 aacgccttca acaccggccg cggccccgcc gccgtattcc gcaccattcc cagcaccgtc
     2941 ttatcgctgg aagaattgaa agccccgagc attttccaaa aaatcgcaga atcgccgcgc
     3001 ggcatggtat tggttaccgg ccctaccggt tcgggcaaat cgaccacgct tgccgcgatg
     3061 atcaactaca tcaacgaaac ccagccggca cacatcctga ccatcgaaga cccgatcgaa
     3121 ttcgtccacc aaagcaaaaa atccctgatt aaccaacgcg agctgcacca gcacaccctc
     3181 agcttcgcca acgcgctgag ttccgcattg cgcgaagacc cagacgttat ccttgtcggc
     3241 gagatgcgcg accccgaaac catcggcttg gcactgaccg ccgccgaaac cggacacttg
     3301 gttttcggca cgctgcacac gaccggcgcg gcaaaaaccg tcgaccgtat cgtggacgta
     3361 ttccccgccg gagagaaaga aatggtgcgt tccatgctgt ccgaatcgct gaccgccgtc
     3421 atctcccaaa acctgctgaa aacgcacgac ggcaacggcc gtgtcgcctc gcacgaaatc
     3481 ctgattgcca accccgccgt ccgcaacctc atccgcgaaa acaaaatcac gcagattaac
     3541 tccgtcctgc aaaccgggca ggcgagcggt atgcagacga tggaccaatc gctgcaatcg
     3601 ctggtgcgcc aagggctgat cgcaccggaa gccgcacgca gacgcgcgca aaacagcgaa
     3661 agtatgagtt tctgacacac aaccgccttc cggccatacc ggcgggaaaa caaggcgcaa
     3721 acacgccggg cgggacgcgg catcccgccc ggcataccct tccgaacagg aagcgtccgc
     3781 cttcctgttg aaacctgccg ccgcaaactg caaggcttaa accgaaaaga agctaacgat
     3841 gaataccgat aacctgcacg acatcttgga cgaaacggtt caagtgtatt ctcaaaaaaa
     3901 acaaagccga tccgaaaccc cggccgaaat cggcgcacac ttccacccgc tgctcgaccg
     3961 cctgtgcgaa accgcagaag cacaaaacgc gtccgacatc cttatcagca aaggattccc
     4021 gccctcgttg aaaatcaaca gcgcattaac cccgcagccg caaaaggctc tgacgggcga
     4081 ggaaaccgcc gccatcgccg catcgacgat gaacgccgaa caatcggaaa tattccggcg
     4141 cgacggcgaa atcaactact ccgtccaatc gcgcagcggc acgcgctacc gcgccaacgc
     4201 ctactacagc caaggcagcg caggtttggt tttgcggcgc atcaaccacg tcatcccgca
     4261 aatgcgggaa ttgggcttgc ccgaaaaact caaagacctc gccgtcgcac cgcgcgggct
     4321 gctgattatc gtcgggccta ccggttcggg caaatccacc acgatggcga ctatgctcga
     4381 acaccgcaac aaaaccctgc ccggccatat cgttaccatc gaagacccga ttgagtttat
     4441 ctacaaaccg cgccgctgca tctttaccca gcgcgaaatc ggcgtcgaca ccataaactg
     4501 gcagacggcg gtacaaaacg ctatgcgcca atccccagac gtggtctgta tcggcgaagt
     4561 ccgcagcagg gaaagtatgg aatacgcgat gcagctcgcc caaaccggcc acctgtgcat
     4621 ttttacgctc cacgccaaca ccgcgccgca gtcgctcgaa cgcatactca acttctaccc
     4681 caaagaacag cacaaccaaa tactgattga catcgccctc aatctgaccg gcatcatctg
     4741 ccaacgcctc gccctcaaaa aagacaaaac gggcaggacg gcggttgtcg acttgctcat
     4801 caacacgccc gccatccaag acttcatcct gaagggcgac ctgatgaaca tcagtaaaat
     4861 catggaaacc gccaaaaccg acggaatgca gacgatggac caaaaccttt tcgaactgta
     4921 ccgtcacggc atcatcagtt acgaagaagc cctgcgccag tccgtttccg ccaacaacct
     4981 gcgattgcac atccaactgc acaaagaagg caaaacgccc gaactccttt acgacagggt
     5041 caacggtctc aacctcattt cctgatccgc aaaacccaat gccgtctgaa aaccgcatcc
     5101 ccgttttcag acggcatgat tttatccgcc ccattcatgt gccacacttt attcacttca
     5161 taacaataaa ccggtaaaac catgaaaacc ccactcctca agcctctgct cattacctcg
     5221 cttcccgttt tcgccagtgt ctttaccgcc gcctccatcg tctggcagct aggcgaaccc
     5281 aagctcgcca tgcccttcgt actcggcatc atcgccggcg gcctggtcga tttggacaac
     5341 cgcctgaccg gacggctgaa aaacatcatc gccaccgtcg ccctgtttac cctctcctcg
     5401 ctcacggcgc aaagcaccct cggcacaggg ctgcccttca tcctcgccat gaccctgatg
     5461 accttcggct ttaccatttt aggcgcggtc gggctgaaat accgcacctt cgccttcggc
     5521 gcactcgccg tcgccaccta caccacgctt acctacaccc ccgaaaccta ctggctgacc
     5581 aaccccttca tgattttatg cggcaccgta ctgtacagca ccgccatcat cctgttccaa
     5641 atcatcctgc cccaccgccc cgtccaagaa agcgtcgcca acgcctacga agcactcggc
     5701 ggctacctcg aagccaaagc cgacttcttc gaccccgatg aggcagcctg gataggcaac
     5761 cgccacatcg acctcgccat gagcaacacc ggcgtcatca ccgccttcaa ccaatgccgt
     5821 tccgccctgt tttaccgttt gcgcggcaaa caccgccacc cgcgcaccgc caaaatgctg
     5881 cgctactact tcgccgccca agacatccac gaacgcatca gctccgccca cgtcgactac
     5941 caagagatgt ccgaaaaatt caaaaacacc gacatcatct tccgcatcca ccgcctgctc
     6001 gaaatgcagg gacaagcctg ccgcaacacc gcacaagccc tgcgcgcaag caaagactac
     6061 gtttacagca aacgcctcgg acgcgccatc gaaggctgcc gccaatcgct gcgcctcctt
     6121 tcagacggca acgacagtcc cgacatccgc cacctgagcc gccttctcga caacctcggc
     6181 agcgtcgacc agcagttccg ccaactccga cacagcgact cccccgccga aaacgaccgc
     6241 atgggcgaca cccgcatcgc cgccctcgaa accggcagct tcaaaaacac ctggcaggca
     6301 atccgtccgc agctgaacct cgaatcaggc gtattccgcc atgccgtccg cctgtccctc
     6361 gtcgttgccg ccgcctgcac catcgtcgaa gccctcaacc tcaacctcgg ctactggata
     6421 ctgctgaccg cccttttcgt ctgccaaccc aactacaccg ccaccaaaag ccgcgtgtac
     6481 caacgcatcg ccggcaccgt actcggcgta atcgtcggct cgctcgtccc ctacttcacc
     6541 ccctccgtcg aaaccaaact ctggatcgtc atcgccggta ccaccctctt tttcatgacc
     6601 cgcacctaca aatacagttt ttccaccttc ttcatcacca ttcaggcact gaccagcctc
     6661 tccctcgcag gtttggacgt atacgccgcc atgcccgtgc gcatcatcga caccattatc
     6721 ggcgcatccc ttgcctgggc ggcggtcagc tacctgtggc cagactggaa atacctcacg
     6781 ctcgaacgca ccgccgccct tgccgtatgc agcagcggca catacctcca aaaaattgcc
     6841 gaacgcctca aaaccggcga aaccggcgac gacatagaat accgcatcac ccgccgccgc
     6901 gcccacgaac acaccgccgc cctcagcagc accctttccg acatgagcag cgaacccgca
     6961 aaattcgccg acagcctgca acccggcttt accctgctca aaaccggcta cgccctgacc
     7021 ggctacatct ccgccctcgg cgcataccgc agcgaaatgc acgaagaatg cagccccgac
     7081 tttaccgcac agttccacct tgccgccgaa cacaccgccc acatcttcca acacctgccc
     7141 gacatgggac ccgacgactt tcagacggca ttggatacac tgcgcggcga actcggcacc
     7201 ctccgcaccc gcagcagcgg aacacaaagc cacatcctcc tccaacagct ccaactcatc
     7261 gcccggcagc tcgaacccta ctaccgcgcc taccgacaaa ttccgcacag gcagccccaa
     7321 aacgcagcct gaaaaaattt cggcattttg taaagggggg cggattgtca ggcaggttgc
     7381 aagatagtgg ataaggcttt gcgcagggta aatgcgtagc aactgaaccg tcattccctc
     7441 aaaaacagaa aaccaaaaac agaaacctaa aatcccgtca ttcccacgaa agtgggaatc
     7501 cggttttttg agtttcagtc attcccgata aattgcctta gcattgaatg tctagattcc
     7561 cgcctgcgcg ggaatgacgg cggagcggtt tctgtttttt ccgataaatg ccgcaatctc
     7621 aaatcccgtc attcccacga aagtgggaat ctaggtctgt cggcacagaa acttatcggg
     7681 taaaacggtt tctttagatt ttacgttcta gattcccgcc tgtgcgggaa tgacggttta
     7741 gaagttgccc gaaacctcaa aaaaaaccga aaccgaacaa gccggattcc cgcctgagcg
     7801 ggaatgacgg gctaaataat atcaaaccat aaatcccgcc aagaaacatt attttcttca
     7861 atcagttgca atttccaagc cctgttccat ttcttcaact gtttttcccg agtaattgcg
     7921 ctctccatcg taggatgcag ttcataccaa accggcatag taacgttgta ccgtgatgta
     7981 aatccttcaa tcaaatgctc cctatgttgg taaatacgtt gcaccaaatc agatgtaacg
     8041 ccaatgtata acgtgccatt acgttggctt gctaaaatat aaaccgtgca tataataccc
     8101 ttttgaatta tttaaattta tattcccgcg aacaccatcc ggtgattact ttaacccttc
     8161 gttatcccca tagctttcca tcattcccgc aactcttcgt cattcccgcg aaag
//
LOCUS       DAWTHD010000095         8116 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWTHD010000095 DAWTHD010000000
VERSION     DAWTHD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.095"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..886)
                     /locus_tag="WM704_001730"
     CDS             complement(<1..886)
                     /locus_tag="WM704_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFA9341097.1"
                     /translation="MSDLVRYNPLEHGRLAGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGVLEDVGEMAGAVLEQAPNALVPLATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIARGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFAALAKE
                     SAKGGLGGAARHAAAYATE"
     gene            complement(883..2079)
                     /locus_tag="WM704_001731"
     CDS             complement(883..2079)
                     /locus_tag="WM704_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341098.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPYAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQEGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAQLGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(2316..4568)
                     /locus_tag="WM704_001732"
     CDS             complement(2316..4568)
                     /locus_tag="WM704_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341099.1"
                     /translation="MGTDVPETGVLPDKNGEPLTIGEYRLFVGEMMNQPAWRAVADKE
                     MDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTADGETGG
                     RDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYECGVIH
                     RNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDWRISGE
                     MLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRDKKTGR
                     TVEFDGANPNHREMAANGAVLFAASVPRMRRAFVVGDLVVRDEPTPYPHQKFPYVPFF
                     GFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVRTKGIVDMSDEQFRRNI
                     ARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRATIRQISGITPSFMGNR
                     GNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMIIEDLGSDEQTVVIEGD
                     AVTQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPSTNSYRSQQLGAMSEAV
                     KSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKEVRVQETPEQIEARIAQAVQDALAKS
                     GNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQAGGQIAAMPQIAPVADAV
                     MQGAGYVRPARGDDPGFPVPAMPPETQIPPEGIPEAYGADTGPMTAVPPKSANHAQTG
                     METPTVSDNL"
     gene            complement(4568..5842)
                     /locus_tag="WM704_001733"
     CDS             complement(4568..5842)
                     /locus_tag="WM704_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFA9341100.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCVREIQKSMRDTVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMNKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     ATGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            complement(5823..6065)
                     /locus_tag="WM704_001734"
     CDS             complement(5823..6065)
                     /locus_tag="WM704_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341101.1"
                     /translation="MAENLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIE
                     RKSYGMDTAESKISESAKTAGIRIEFVGPEDDGKDG"
     gene            complement(6123..6362)
                     /locus_tag="WM704_001735"
                     /pseudo
     CDS             complement(6123..6362)
                     /locus_tag="WM704_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6362..6784)
                     /locus_tag="WM704_001736"
     CDS             complement(6362..6784)
                     /locus_tag="WM704_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341102.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(6849..7367)
                     /locus_tag="WM704_001737"
     CDS             complement(6849..7367)
                     /locus_tag="WM704_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9341103.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(7358..7741)
                     /locus_tag="WM704_001738"
     CDS             complement(7358..7741)
                     /locus_tag="WM704_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFA9341104.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(7738..8088)
                     /locus_tag="WM704_001739"
     CDS             complement(7738..8088)
                     /locus_tag="WM704_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HFA9341105.1"
                     /translation="MRQTRVGISSDRRVGMSSAIRKAAKGGQCTPNIAGVCNDNPETV
                     VLCRFPGETHGAGLKSGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRR
                     LEALGVVGVKGRLK"
BASE COUNT         1638 a         2729 c         1838 g         1911 t
ORIGIN      
        1 cttccgtcgc ataagccgcc gcgtgccgtg ccgcaccgcc caaaccgccc ttggcagact
       61 ccttcgccag cgccgcaaat tcaggcgttc ccttagccgc cgcaaccgcc gccttatccg
      121 ccgccgcaac acccattttc tccaatgcgg ctttcccggc ggccttcttg cccatattca
      181 aaatactgcc gccaagtttc atcgccgcca tatccgccgc gccgaccacc gcgcccttga
      241 ccgccgcatt tttcaacgca cccggcgcac ctcgggcgat aaacgccatc accgcatcct
      301 tgtccgcagg gtcgacgccc gccgcctctg ccgccctgtc cagctgcccg ccgtattcca
      361 tcagcgtatt gcccaaggtc gcgcccgcat aggcgccgac agccgcgtta ccgcccgcca
      421 aagcgcccaa tatgccgccg gcggttgtcg tagcaagcgg gacaagcgca ttgggcgcct
      481 gctccagcac cgcacccgcc atctcgccca catcttccaa gacaccgtcc aaaccctttt
      541 cgcgccagtc cttcttaagc tccccccaca cattcgacag gccgccgccg acacccccgc
      601 ccttttccca agcctcgccc aactccctgc gcgcttggga tttgcgcccc tcctgaatct
      661 tccgataatc catatcctcg gccttgagcc ggcccagttc ctctttgtcg cccgtaaggg
      721 cattccaccc catacgcgcc gaacgcatag aatttttaaa cccgcgcgtc aacgccgtat
      781 cgtcggcggc cgcccgcgca tccttttgcg taaagccgcg gtactccttc aaacccccgg
      841 caagccgccc gtgttccaac ggattgtatc tgaccaaatc actcattgct caaccctctt
      901 ctcacccttc tcattctcat aaatgatttt accgccggcc gttttctgac cgttcggctt
      961 aaagcccaac tgcgcataac gcttctcgcg ctctcccgca aactccacat ccaaaggctt
     1021 gcccgtccgc aaatcgacaa tcccctcgcg cttcgccgtc aagcccgtat ccgggtcggc
     1081 aacctcataa ctgatagtct tgaacacatt cgggtcaaag cccttctccc cttgccgctc
     1141 aaacttcccg ttaagcgcat caatctgccg cgcaatctcc ccgcgctgcc cgtccgactt
     1201 cgcaaaacca taagcccgcg tcaaatcggc gatgcgcttc ctatcctcaa gctccagcgc
     1261 atccgcctca cgcttcatct caaacccctg ccgcttcatc cccagctcgg cctgctgcac
     1321 atcaaacgcc ctgtccgcat tcctctgcgc catctcgcgg ttcaacgcac cctcgttcgc
     1381 cttaaccgcc gcatcaaacc gcccctcctg caacaccgcc tcacggttcg ccgcattctc
     1441 cgccgcagcc gccgccagcc tttccctgcc caattccctg accgccgcat ttttattatg
     1501 ttccgccaca atcccgcgcg ccgcattaag ctgcgccgcc gtcaactgac cgttcaaagc
     1561 ccccgcataa ggcgtagtcg ccctcctgta aagctcccgc tcctcaggcg acagcgcatc
     1621 cggcccggca gacaccaaag gacgctgcgc gtcaaaatca cgcaccggtt tgccgtcccg
     1681 atacacattc ccgtcaaatc ccaaaggcgg cggcgtccgt ccgcccgcat ccgtcgggcg
     1741 cgtatcgcgc cgcgcgaaat ccgccccggc ccgcgcatcg ggggaaagcg ccttatcgcc
     1801 gcccgaaaac cccaaaccgg catccgcacc ccgttcgaaa gcacccgccc ggcgcaaccg
     1861 ccgcgcccca tcctcaatat tgttacggct caaatccatc acattccgat aacgcgccgg
     1921 cccggcctca taattcgcaa cggtacgccg caccgcatcg gcacggtgcg cctgtgccgc
     1981 cgcccgctcc cgctcctccg cccccttccg ggccttctct tttttctcct tttccttcat
     2041 ctcccaaaaa tccccggcag acggctcaaa caaacccatc gcaaaccctc cgaacaaata
     2101 aagcccaaaa taagcaaaaa cccaaaccca atcaaacgcc taaagttaag aacaaacaaa
     2161 aatcgcaacc acaaaccaat taataaacaa caagttacac aaaaaaacac accgacgggc
     2221 catttccggg ggcaaataaa aaccgccttc gtgcaaggcg gttgcaaaaa agcctttcat
     2281 ttaacgaaag gctttttgcc gcccgcgtac cgcgttcaga ggttgtccga caccgtcggc
     2341 gtttccatgc ctgtttgggc gtgattcgca ctctttggcg gcacggccgt catcgggccg
     2401 gtatccgcgc cgtaggcttc agggatgcct tcgggcggta tttgcgtttc aggcggcatg
     2461 gcggggacgg ggaagccggg atcgtcgccc cgcgccggcc tgacatatcc cgcgccctgc
     2521 atgaccgcgt cggcaacggg ggcgatttgc ggcatggcgg ctatctgccc gcccgcctgc
     2581 atggccgcat aagccgcctg cacgccgatt tgtaccgccc gcgcttcgat ttccttgatt
     2641 tcgctcgcgg tacgttgttc cttgagcgcc aattcccgcc gtttgatgtc gttgccggat
     2701 tttgccaatg cgtcctgcac cgcctgcgcg atacgcgcct cgatttgttc gggcgtttcc
     2761 tgcactcgga cttctttgat tttttcaatc actttgtctt taaacgggat gtccatcagg
     2821 gacaccataa acggcagcac cgccgcctga tattcgggcg gcagggattt gaccgcctcg
     2881 ctcatcgcac ccagctgctg gctgcggtag gagttggtgc tgggcacgtc ttccaaagcc
     2941 actttcagcc gtatgttttg caggtcgttg gacaaataag ccttgccggt tacggggtcg
     3001 gtttcaggcc tgttgatgac gaccgtccgc ccttgcgtga cggcgtcccc ttctatgacg
     3061 acggtttgct cgtccgagcc caaatcctcg ataatcatcg caagcagcaa ctcgccgacc
     3121 aatgagcggc tctggcggaa gttgtccata accagcccca gcgactggtt ggactgctcg
     3181 acttggatgc tttcctgcct gccgctggtg gcgttgcccc ggttgcccat aaatgacggg
     3241 gtaatcccgc tgatttgccg tattgtcgcg cggctgtctt gaagcatctg ccaatgctgt
     3301 gccgacaatt cgaaatcgcg gctgacgtcg aaacgcgcgc ccggctgggc ggcctctatt
     3361 ttgttcagca cgatgtccgc gtccacccgt gcgatattgc ggcggaactg ttcgtccgac
     3421 atatcgacta tgcctttggt gcgtaccgtg cgtattgccg acaaacccca tcgtaatttg
     3481 ctgttggtgc tgttgaggtt gtcctgcgcg tatttcatat tgcggacata tccgtagggg
     3541 atgccggtgt tgtcctcgcg gaatccgaaa aacgggacgt aagggaactt ttgatgcgga
     3601 tacggggtcg gctcgtcgcg gacgaccaaa tcccccacga caaaggcgcg gcgcatacgc
     3661 gggacggaag cggcaaacaa taccgcgccg tttgccgcca tctcccgatg gtttgggttt
     3721 gcgccgtcaa actccaccgt gcgccctgtt tttttgtcgc gcaggcagtc tgccgttacc
     3781 caccgcctgt accatacttc cgccaccgcc agttcgcgcg tggtttcatt gaaccaaaac
     3841 tcttcgctga cggtgttgcg ccccgaaata ccccaagcgt ccgccagtcc ggtattgccg
     3901 ccgccgtcaa gcatttcccc gctgatgcgc cagtccgaac cgccgcgccc catcgctttg
     3961 aagtgtccgg catattcagg gaagaattgc gccaggcgtt ccggcagcag ccagcggcgg
     4021 cgtatcagcc agcgggcatc ggacaggtcg tatttgtaag atttcatatc ccaatggatg
     4081 gcgttgcggt ggatgacgcc gcactcataa ggaaactcga aagggttggg gttgcgtgta
     4141 acctccaccc agccgatgcc gcaggctatc tgccccctga acgcgtccga acaagccttg
     4201 tcggcacggc tttgccgttc cgcgcggttg agtttgaagt tcaatgcgtc cgccacgtcc
     4261 cgcccgccgg tttcgccgtc cgccgtcacg cgccagtccg tccgtatcgt cgcctcatat
     4321 ccctgtaccg acagcagggt cggggtaatc aggttttcaa cggcgggggg caggcccaac
     4381 tcgcgctgtt tctgtaaaag ctcgttgtca agctgcctgc cgtcggcgta gtccatttcc
     4441 ttgtcggcaa cggcacgcca tgcaggttgg ttcatcattt caccgacaaa cagccggtat
     4501 tccccgatag tcagcggttc gccgttttta tcgggcaata cgcctgtttc cggtacgtcc
     4561 gtccccatca ataaagcctc caatccgaaa cgggcgtttc cgtgtatccc gtattgctgc
     4621 ctgtatccat taaatcaacc gcctgcgcca ggtagcggaa catatccgcg ccgtgcgaat
     4681 attcgtcgtg cagcggcccc attgccacgc ctgttttcgc atgtatttgg cggccgtacc
     4741 gtttcaggca ttccaaaagc cgcgctgttt tgtctttatc gaagtacact ttgggaaaca
     4801 gcatccgcgc cgccctgatg ccttcttcga taccggtcgc gttttgcacg aaaaccgact
     4861 tgcgccccag tccggtcaaa atctccatcg tacttttgcc tgtttggaag ttgcgcgtcc
     4921 tgccgtcgtg cggcaggaag tccgtccccc accggtaggg cagcttttcc aattcggcaa
     4981 cataccagtc caacgtccgg tgcgtgtctt cgatgtagcc gatgatgcgc acgctcgtca
     5041 aatcgcgctg caccagcccg atggtcatgg catcgttcca gcccaaatcc caaaccgtat
     5101 gcacgggcaa agaagaatca taagggacga gtgtaacgcg tccggaatga aaagcgtcct
     5161 gtatttcatg ccggtaaacc gccccctccg ataccatgcg cggcctgcct tcccaaatat
     5221 tcccgtagtc ctctttattc atcgaacgct gtgctttgag ccgctcccgg ttcaatgctt
     5281 cgggaaacca cggattgtcg cgccagttga tttcgcacaa ccaagtgtct tcggacggca
     5341 tagcgataaa acgccggtag gtttcgtccg tctccatatc gggattgagg gtaatccaaa
     5401 tttccgaacc ttctttgcgt atggtcggcg tgagcacgtc ccagcttttt ttactgacgc
     5461 cgtgcccttc ctcaacccat acgatgtcga taccttcaaa cgatttgatg ctgtccacgg
     5521 tatgcgactg caggcccgaa aacacgaaca gcgtgccgtt tgcgccgcgt atctcgaagt
     5581 cggttatttc gtagaaatgc cccaaaccca actgcgccac tttgtctttc aacaggcggt
     5641 gtacggtatc gcgcatcgat ttttgaattt cgcgcacgca taaaatacgc aaagggcgtt
     5701 gcgcccccag cgccagcagt gcggatgccg cgccgtgcga tttgccgccg ccgcgcccgc
     5761 cgtacataac cttgtaccgg caaggcttga acagcccgtc cagttttgcg ggcaatttca
     5821 aatcaaccgt ctttcccgtc atcttcaggc cctacaaatt cgatacggat gcccgccgtc
     5881 ttggcggatt ccgaaatctt cgattccgcg gtatccatac cgtatgattt acgctcgatt
     5941 tccaccaaaa ccttaaaccc cgccgccacc gtttggaaca tacgcgcctt tgtgaatgtg
     6001 tcctccgggc ttccgtacaa ttccgcgaca atctgctccg cgcttttgga caggttttcc
     6061 gccaaattcc gatgcctgcg ctgtacggcg gcaacccgtt tcgcttccgc gtccgttact
     6121 tgtttaaggt tgaaatccgc ataatccggc gcattgccgt ttttgtccgc ggacagcacc
     6181 atcgcccgcg cctttgcctg tacctcgtca ttcaaatcgc gcgtccacac gcgttctttg
     6241 gcaaccctgc cgacgtgttg cgccgaaaca ttgtatttgc ggccgatttc ggcaatgctc
     6301 aaaaccccgc gccggtaatc cagctcgact ttgcgccaat cttcatctga caatctcggc
     6361 attaaaggtc caacctcccg tttttggcgg aacattccag ataattccgc aatactgaaa
     6421 actcgcgccc catctgaata ctggaaaact gctgcccgaa ccccgacatc aaagccccca
     6481 acgagcgcat ttcctgcggg ttgaggtgca ataccgtatc gcctatttcc aatacgccca
     6541 tccccgaatc caataccgat acgctgacgg ggcgcgtctc gccaaaccct atggcgggct
     6601 tgaaaatccc ccttgtcacg cggtagattt tgccgtgctc gaccagcagc ttggtaaatt
     6661 tagtgatttc cgacattttc aggcctgtcg tttccgccaa agcctcgcgg ctgaccacct
     6721 gctccgcgtt gtgcaaatcc acaaccccct ggtacacctt gtcaatatcc gacaatcccg
     6781 acatcaaatc aaactcctaa acatcccaaa cgattccaaa ctccgccgcc gcccgcgatt
     6841 gcaggcggtt acactacaaa gccaaaatct ttagatgctg cctctctcgc tttcactgca
     6901 tcttcaatat gttcaaataa acctaatatt ttattttttc tattaaccat aatcctagct
     6961 tgccatttct tagacggctt atggaaataa accccagtat gcccgctagt attattttta
     7021 aacttccctc tatttttcat attttcagca tgtgtaacat ctctcaaatt agatattcta
     7081 ttatctgtct tatccctatt gatatggtct attgaagaag cgtgtttccc ataaacaaaa
     7141 agccaaatta atctatgtgc attatattgt ttcttaaata tttttaactt caaatacccc
     7201 aacctgcctt tgtataagaa aacctccttg ccggaaaatc cggtattcca agatttgtaa
     7261 tgcaaaccgc tattgaaata ctttcttgga cgctccgccc aatataactt tcccgtattg
     7321 tcatcatatc ttaataattc ttttaaaacc tcttgtgtta atcccataaa acaccatgct
     7381 ccatagatgc ccaagatata atcttttctt gatattcaga catttcccga actgataatt
     7441 ttgtcgttga tatacccatt aattgcccgt taggcatttc aatacatcca ataaattttc
     7501 tttttaaaaa ttcatgccat acatcttgac taaaccttct cccatcgata aaaacttttt
     7561 ctgaaatttc acgatacaaa aaccatagcc gcctgttctg ctcgacgctc cgttttgact
     7621 tgcgcgggcg gattgtgatt tcaaggttgc cgttttcaaa ccacccgtcc gggctgtccc
     7681 aaatcgaccg cataacctgc tttttgtttt ggggcgtcag tgtaaattcc gccccgttca
     7741 tttcagacgg cctttcacgc cgacaacccc caatgcttca aggcggcgta tcgtgcgcaa
     7801 ttgcgaacgg cgcatataaa actctttgtc ttcgcggctt aggcctgcgc ccctgccgtc
     7861 gatcgcgccg cggcggcaac cgcacccgaa acccgcgccc aaaccgccgc ttttcaatcc
     7921 cgccccgtgc gtctcgcccg ggaaacggca caacacgacc gtttcagggt tgtcattgca
     7981 cacgcccgcg atgttcggcg tgcattgccc gcctttggcg gctttgcgta tcgcgctaga
     8041 catcccaacc ctcctgtccg atgaaatccc aaccctcgtc tgccgcatcc gtctgttcca
     8101 tctccaactc ccccac
//
LOCUS       DAWTHD010000096         8047 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWTHD010000096 DAWTHD010000000
VERSION     DAWTHD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.096"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..477
                     /locus_tag="WM704_001740"
     CDS             <1..477
                     /locus_tag="WM704_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9341106.1"
                     /translation="WKESNSSTKKVTEDIKDNYRETKTEHQGNGSFHAASSLGLSAVY
                     DFKLNDKFKPYIGARVGYGHVRHQVRSVGQETITVTPKPKNGTQGGSVISTSPVPAYH
                     ENRSSRRLGFGAMAGVGIDVAPNLTLDAGYRYRYWGRLENTRFKTHEASLGVRYRF"
     gene            712..1008
                     /locus_tag="WM704_001741"
     CDS             712..1008
                     /locus_tag="WM704_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341107.1"
                     /translation="MQADLAYAYEHITRDYPEATGANQGKISTVSDYFKNIRTRSVHP
                     RLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
     gene            complement(2460..2696)
                     /locus_tag="WM704_001742"
     CDS             complement(2460..2696)
                     /locus_tag="WM704_001742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341108.1"
                     /translation="MKTANPHIVIPAKTALHTPPAAAIFTLKSRHSRESGNLGMKSSR
                     NLSEITETERTGFPPAREWRGKVGCGWDWWNDEI"
     gene            complement(2700..2855)
                     /locus_tag="WM704_001743"
     CDS             complement(2700..2855)
                     /locus_tag="WM704_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341109.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(2872..4668)
                     /locus_tag="WM704_001744"
     CDS             complement(2872..4668)
                     /locus_tag="WM704_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFA9341110.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            5127..6380
                     /locus_tag="WM704_001745"
     CDS             5127..6380
                     /locus_tag="WM704_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFA9341111.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLVAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGREKTGNAELFAWN
                     IWVAGSGLAVIFWFDGAMAELLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            6535..8046
                     /gene="lysS"
                     /locus_tag="WM704_001746"
     CDS             6535..8046
                     /gene="lysS"
                     /locus_tag="WM704_001746"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFA9341112.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDGFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1761 a         2196 c         2263 g         1827 t
ORIGIN      
        1 tggaaagaaa gtaattcttc tactaaaaaa gttactgaag atataaaaga caactaccga
       61 gaaaccaaaa cagaacatca aggaaacggc agcttccacg ccgcttcttc tctcggctta
      121 tccgccgttt acgatttcaa actcaacgat aaattcaaac cctatatcgg tgcgcgcgtc
      181 ggctacggac acgtcagaca tcaggttcgt tcggttgggc aagaaactat aactgttacc
      241 cctaaaccaa agaatggtac gcaaggaggt tctgttatat caactagccc cgtccctgcc
      301 tatcacgaaa accgcagcag ccgccgcttg ggcttcggcg cgatggcggg cgtgggcata
      361 gacgtcgcgc ccaacctgac cctggacgcc ggctaccgct accgctattg gggacgcctg
      421 gaaaacaccc gcttcaaaac ccacgaagcc tcattgggcg tgcgctaccg cttctgattc
      481 gccgattccg atgccgtctg aaccttcaga cggcattttt aaggcgcaag atcgggcaaa
      541 cggcattttc agacggcata actgacagta taatccgaac atccggccgc tttcgcgcgg
      601 ccttcagcat tatccgattt tttccgaaag ccgaaccatg caatacaaac ccctcctgct
      661 cgccctgatg cttggcaggc ggcgggtgaa gaccatgggc gcggcccgta tgtgcaggcg
      721 gatctggctt acgcctacga gcacatcacc cgcgattatc ccgaagcaac cggtgcaaac
      781 caaggcaaaa taagcacggt aagcgattat ttcaaaaaca tccgcacccg ctccgtccac
      841 ccccgactcg ccctcggcta cgatttcggc ggctggcgcg tcttttccgc ccccgccgtt
      901 gccgccggcc tccaccaatc ccttcaatat taccgatccc gccattgccg ccgattccgt
      961 caaatccatc aattccgccg aatcacgcct attccccaaa aaccttgatg cggcgggctg
     1021 aagcccgccc tgcaaccctc tctatgcacc cccttgcgag cccgacacta cgcaacatct
     1081 tgagaaccca tcctgtcaag aatacccgaa ccgtcccgat acaccgtaat cctaaaaccc
     1141 gccattcccg cgcaggcggg aatccagtcc gttcggtttc agtcatttcc aaaaattgcc
     1201 gtagcgttaa gtttctagat tcccactttc gggggaatga cgcggtgcag gtttccgtgc
     1261 ggacggattc gtcattccca cacagacagg aatccggatt tgtccgcgcg gaaacttatg
     1321 cgccgtcatt cccgcacagg cgggaattca ggtctgtcgg cataggaact tatcggataa
     1381 aacggttgcc ccaaccctgc gttctagatt cccactttcg tgggaatgac gattcaggta
     1441 ttcctaatag caacccgcca caacccacct acgcccatcc tacgcatctg ccatacagcc
     1501 ctgttccgga gcgcgcggat aaggtgcgat gccgccggcg ggcatcatag tcctctctcc
     1561 ctgtgggaga gagctagaaa gctgcaaggc ttgtatttta gaagactaag ggatttggga
     1621 aagattgtcg aaattcgggg agccctctcc cccacggggg agggggcagg ttgcggcaga
     1681 tttggcggtt gcaggcggtt tgaaaaacag attggattta caaccgttga tttcaggtcg
     1741 tctgaaaagc aaaaagcagc ctgcacaacc tgattagttt acaaaaccct taattccaac
     1801 agcctccccg tcctctctcc ccgtgggaga gggctggaga gagggcaaca agccgcaagg
     1861 cttgtattgg ggcggttaag ttgttgggaa aggttgccgc aacttggaga atgccgcctc
     1921 cccggccctc cccacggggg agggagcagg ttgcggcagg ttttgcggtt gcaggcggtt
     1981 tgaaaaacag attggattta caaccgttga tttcaggtcg tctgaaaagt aaaaagcagc
     2041 ctgcacaacc tgattagttt acaaaaccct taaccccaac agcctccccg tcctctctcc
     2101 cgtgggagag ggctggagag agggcaacaa gccgcaaggc ttgtattggg gcggttaagc
     2161 tgttgggaaa gattgccgca acttggagaa tgccgcctcc ccggccctcc cccacggggg
     2221 aggggcaggt tgcagcagat ttggcggttg caggcggttt gaaaaacaga ttggatttac
     2281 aaccgttgat ttcaggtcgt ctgaaaagca aaaagcagcc tgcacaacct attttcttgg
     2341 aatagcgcat taaaaagagt atgagaatgg agacataaaa aattccggcg aatgtccgtg
     2401 cgccgttccc gtccgtcatt cccgcacagg cgggaatccg gatttgtccg cacggaaact
     2461 tatatttcgt cattccacca atcccaccca cagcccactt ttccgcgcca ttcccgcgca
     2521 ggcgggaatc cagtccgttc ggtttcggtt atttccgata aattcctgct gcttttcatt
     2581 cctagattcc cactttcgcg ggaatgacgg gatttcaggg taaagatggc ggcagccgga
     2641 ggggtgtgaa gagctgtttt cgcaggaatg acgatatgcg gatttgctgt tttcatccgc
     2701 taccgatgcc gtctgaagcc tgtttggcgt ttcagacggc attgggtttt ttgtgcattt
     2761 gccgcaaaac ggatcggtgt ctgcttgcgg caagacgata acggtgcgga atgttttggg
     2821 aaaccaatgc cgtctgaagg gctttcagac ggcatttttg taccgtgccg tttaacgcgc
     2881 cagcggttcg gtgcgtttga tcagccacgc ttttgccgcg ccttcggtca gcggctcgag
     2941 gcggcggcgg acttcggcgt ggtagcggtt gacccagtcg atttcgccgt cggtcatcag
     3001 ggcggtgtcc atcaggcgtg tatcgatggg gcagagggtc agggtttcaa aacagaggaa
     3061 gctgccgaac tcggtttcct gcgggtcggc gacggcttgg ttggcggcga ggttttcaat
     3121 gcggatgccc catttccccg gacggtagag gccgggttcg atggaggtaa ccatgccttt
     3181 tttcatggcg gtttcgggcg tggcgggggc ggcgaaggcg atgcgctgcg ggccttcgtg
     3241 gacgttgagg aaatagccta cgccgtggcc ggtgccgtgt ccgtagtcgc attgcgcctg
     3301 ccacaggggt ttgcggcaaa tcgcgtcaat cagcggcgag gggatgtttt cggggaacac
     3361 ggcttcggca agcgcgatat gggctttgag aacgagcgtg ttgtcgcgtt tttgttctgc
     3421 tgtgggcgtg ccgacgggga cgacgcgggt gatgtcggtc gtgccgcctt tgtattgcgc
     3481 gccggagtcg atgagcagca gaccgttgcc gctgatggtg ctgtggcttt cgggtgttgc
     3541 gctgtaatgc ggcagggcgc cgttggcgtt gaagcctgcg atggtgtcga aactcaagga
     3601 cacaaaaccc gggcgcgcgc tgcggtggcg gtaaagcatc gtatccacgt cgatttcggt
     3661 caggctgccg cctttgccga tgatgtcttc aaactcggcg aagaagccgc acaacgccgc
     3721 gccgtcttgt tccatcgctt cgcggatgcg ggcgatgtcg gcttcggatt tgacggattt
     3781 gaagaaggtg gacgggttga tgccttcaat caggcgcgcg ctttcgggca ggcgcacgag
     3841 cgtactgacg gcggttttgt tcggctcgat gagcagcgcg ccgccgattt gcgcgagttt
     3901 gtcggcaact tgggcgtaag gctcgaccgt gatacctgcg gtttgcagcg cggcggcggc
     3961 ttcggcgttc agacggcatt gttcggtaaa caggacggca ctgtctttgc cgatcagcag
     4021 gaaggacacg aaaacagggt tgaaaggcac gtcgctgccg cgcaggttgg tcagccaagc
     4081 gatgtcgtca agcgaggaaa ccaagtggta atccgcgcct ttttccgcca taactgcgcg
     4141 cacgcgggcg agtttctcgg cggcggtttc ggagacgtag gcgtggtcgt ggatgaacac
     4201 ggtttcggcg gggatagcgg gacggcttgt ccacacttgg tcaagcaggt cgtcggggtg
     4261 ttggatgcgg atgtttttgg cggcgagcga ttgcgccaaa gtgcgtttgc cggtgagcga
     4321 caccatatcg gaagggatgc cgacggcagc gttttcgggc aggtttgccg cgagccattc
     4381 gttgtacggc ggcacttgcc cgcttttttg cagctcaatg ccgctgccgg aaagctgttt
     4441 ggcggcttgt tcccaatagc ggctgtccac ccatacgccc gcttcatcgg cggtaacgac
     4501 gaatgtgccg accgagccgg taaagcctga caattcgcgc cgcgcctgcc aatgctcggg
     4561 caggtattcg gataggtggg ggtcggcgga agggatgacg agtgcgtcca agccttgcgc
     4621 cttcatggct tcgcgcaatg cggacaggta attcgatacg gtattcataa cagttcctcc
     4681 aatcgggttt cgcggcttca gacggcatgg acggaaatct gaaatgccgt ctgaaaaaca
     4741 taaccatatt atcaaatcgc cagcctgctg caaaacggac gggctgatcg gatggtgcgt
     4801 gtttgcacga aaaaaagcga tgtaaggttg tttgttgtaa aatttcaggc ataaaatgtc
     4861 gtttcatgcc ttcttttgga agtaatacgg aaaatatagt caggtttcag catgaagttc
     4921 taatagtgtg tgttggcagg tatttggatt acgttacaat acgcgctttc atcaaaattt
     4981 ccctaccggg cggtatcctg tttttgaaga cggtaggatt tgggtttgtt tggggtcaat
     5041 cccgcccaaa ttggagcaat agatagggct gtgtccgaat atgcggctct gtatgttgaa
     5101 acacagttaa aaatacggaa gactttatgt ccgaacaaca tatttcgact tggaaaagca
     5161 agatcaacgc attgggaccg gggattatga tggcttcggc ggctgtcggc ggttcgcacc
     5221 tgattgcctc gacgcaggcg ggcgcgcttt acggctggca gctcgcgctc atcatcatcc
     5281 tgaccaacct cttcaaatac ccgtttttcc gcttcagcgc gcattacact ctggacacgg
     5341 gcaagagtct gattgaaggt tatgccgaga aaagctgcgt ttatttgtgg gtttttttga
     5401 ttttgtgtat cgcctccgcc acgattaacg cgggcgcggt cgccattgta accgccgcca
     5461 tcgtcaaaat ggcgattccc tcgctgatgt ttgatgccgg cacggttgcc gccttgatta
     5521 tggcatcctg cctgattatt ttggtgagcg gacgttaccg cgctttggat cgcgtttcca
     5581 aaatcatcat cgttactttg agtatcgcca cgcttgtcgc cgccggcatc gctatgtcgc
     5641 gcggtatgca gatgcagccc gattttatcg agccgacacc gtggacgctt gccggtttgg
     5701 gcttcctgat cgcgctgatg ggttggatgc ccgcgccgat tgagatttcc gccatcaatt
     5761 ctttgtgggt aaccgaaaaa caacgcatcc ggccttccgg ctaccgcgac ggcattttcg
     5821 atttcaacgt cggttatatc gccagtgcgg ttttggcttt ggttttcctt gcactgggcg
     5881 cgtttgtgca atacggcaac ggcgaagcag tgcagatggc gggcggcaaa tatatcgggc
     5941 aattgattaa tatgtatgcc gtaaccatcg gcggctggtc gcgcccgctg gtggcgttta
     6001 tcgcgtttgc ctgtatgtac ggcacgacga ttaccgttgt ggacggttat gcgcgcgcca
     6061 ttgccgagcc cgtgcgcctg ctgcgcggca gggaaaaaac cggcaacgcc gaactctttg
     6121 cctggaatat ttgggtggcg ggcagcggtt tggcggtgat tttctggttt gacggcgcaa
     6181 tggcggaact gctcaaattt gcgatgattg ccgcctttgt gtccgcccct gtgttcgcct
     6241 ggctcaacta ccgcctcgtc aaaggggaca aacgccacag gcttaccgcc ggtatgaacg
     6301 cccttgccat tgtcggcctg ctctacctgg ccgggtttgc cgttttgttc ctgctgaacc
     6361 tgacggggct tttggcatag tttccaaaac acggatgccg tctgcaagat tttcagacgg
     6421 catcgccctt tcttggaaaa aaacaccgac atcgcgtaaa atacgcgcaa attttgtgat
     6481 tcggtcaggt cgtctgaaac agactgcgcc tgatttattt tttcggaaaa ccctatgagc
     6541 gaacaaaacc atccgcaaac cgagccgcag ttggacgaaa accaaatcat cgccctgcgc
     6601 cgcgaaaaac tgaacaacat ccgccaacag cgcaacgctt accccaacga tttcaagcgc
     6661 gacagctttg ccgccgattt gcaggcgcaa tacggcgaaa tcggcaaaga agaactcgac
     6721 ccgcaggccg ttcccgttaa aattgccggc cgcatgatgc tcaaacgtca aatgggcaaa
     6781 gccagcttcg ccaccattca agacgttacc ggtcaaatcc agctttatct gaacaacaaa
     6841 ggcgtgagtc aggaagtttt ggacggcttc aaccactggg acttgggcga catcgtcggc
     6901 gcggaaggca ctttgttcaa aaccaaccac ggcgaactaa ccgtgcgtgt gtccgacatc
     6961 cgcctgctgt ccaaatccct gcgtccgctg cccgacaaac acaaaggctt gagcgatcag
     7021 gaaaccaaat accgccaacg ctacgtcgat ttgattgcca atgaagaatc gcgcaatacc
     7081 tttatcaaac gcagccaaat catccaatct gtgcgtaatt ttatggtggg cgagcattat
     7141 ctcgaagtcg aaaccccgat gatgcacccg attcccggcg gcgcgacagc gaaacccttc
     7201 gttacccacc acaatgcctt ggacattccg ctctacctgc gcatcgcgcc cgagctgtat
     7261 ctgaaacgct tggtggtcgg cggcttggaa cgcgtgtttg aaatcaaccg cagcttccgt
     7321 aacgagggca tgtccgtgcg ccacaacccc gaatttacga tgattgaatt ttacgaagcc
     7381 ttctccgact acgaacgtat gatgcagatg gcggaagaca tcatccgcaa cgcatcgcgc
     7441 acggtaaacg gcacggcaaa catcacttac aacggcaaag aagtcgattt ggaaagcccg
     7501 ttcgaacgtc tgaccatcct cgaagccatc aaaaaataca atccgcacta caccgacgag
     7561 cagttgaacg atgcggaatg gctgaaaaaa gaaatcgtca aacacggcga aagcctgccg
     7621 ccgtccccgg gcatcggcag cctgcaactc gccctgtttg aaggttgcgc cgagggcaaa
     7681 ctgtggaatc cgacctttat tgtcgattac ccggtcgaag tttccccgtt ggcgcgcgct
     7741 tcggatacga aacaaggtct gaccgaacgc ttcgagcttt tcgttgtcgg gcgcgaactg
     7801 gcaaacggct attccgagtt gaacgacccc gaagaccaag ccgaacgctt caaatcgcaa
     7861 gtggcgcaaa aagatgcggg cgacgacgaa gccatgcact acgatgccga ctacatccgc
     7921 gcgatggaat tcggtttgcc gccgaccggc ggttgcggca tcggcatcga ccgcttagtg
     7981 atgctgctga ccgatttgca aaccatccgc gatgtgattc tgtttccgca gatgcgtccc
     8041 gagtaat
//
LOCUS       DAWTHD010000097         7996 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWTHD010000097 DAWTHD010000000
VERSION     DAWTHD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.097"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(122..2158)
                     /locus_tag="WM704_001747"
     CDS             complement(122..2158)
                     /locus_tag="WM704_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFA9341113.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSESDECR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(2246..3118)
                     /locus_tag="WM704_001748"
     CDS             complement(2246..3118)
                     /locus_tag="WM704_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFA9341114.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            3384..5774
                     /gene="gyrB"
                     /locus_tag="WM704_001749"
     CDS             3384..5774
                     /gene="gyrB"
                     /locus_tag="WM704_001749"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFA9341115.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQELATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHQFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPTVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            5873..7258
                     /locus_tag="WM704_001750"
     CDS             5873..7258
                     /locus_tag="WM704_001750"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFA9341116.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7329..7427)
                     /locus_tag="WM704_001751"
     CDS             complement(7329..7427)
                     /locus_tag="WM704_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFA9341117.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            7724..7873
                     /locus_tag="WM704_001752"
     CDS             7724..7873
                     /locus_tag="WM704_001752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341118.1"
                     /translation="MNQIIFAETLKNLPYLPDRHQDFVVVTEKNILKVRIPNQSLKYR
                     NHIRF"
BASE COUNT         1863 a         2254 c         2212 g         1667 t
ORIGIN      
        1 cggcacagaa acttatcggg taaaacggtt tcttcaattt tatgttccgg attcccgcct
       61 gcgcggggat aacgattcag gtatttctac atcgaatccg ctttttatat caacctgact
      121 atcaagccgc gttgtcgaag ccgctgtggc gcagcagtgc gtctatgctc ggttcgcgtc
      181 cgcggaaggc cttgaaggat tccgccgcgc tgcgggagcc gccgacggcg aggatttcct
      241 gccagaagcg tttgcctgtg gcggcaacgt cgtcgctttc ttcaaaggcg gcgtaggcgt
      301 cggcgctgag gacttccgcc catgcgtagc tgtaatagcc tgcggagtag ccgccggcga
      361 agatgtggcc gaagctgttg gcgaagcggt tgtattcggg cggttggatg acggcgactt
      421 ctttgcgcac gctgtctaaa acctgctgcc agtttttcag acggcattcg tcgctttcac
      481 tgtaaatcat catatcgaag agggcgaact ccatttgccg gacgaggaac ataccgcgct
      541 ggaagttttt ggcggcgagc attttgtcga agagttcttt cggcaggggc tcgccggttt
      601 cttcgtgggc ggacatttgt gccaagacgt cgtattccca gacgaagttt tccataaact
      661 ggctgggcag ctcgactgcg tcccattcga cgccgttgat gccggacacg cccagttcgt
      721 ccacttgggt aagcaggtgg tgcagtccgt ggccggtttc gtggaagagg gtgaggattt
      781 cgtcgtggct taaacgcgct tctttgccgc cgacgggcgg ggcgaagttg cagacgaggt
      841 aggcggtggg cagttgcagc gtgccgtcgg caaagcggcg gcggcctttg tagtcgttca
      901 tccacgcgcc gccgcgtttg ccttcgcgtg cgtacaaatc catataaacg ccgccgatgg
      961 tttcgccgtt ttgttgcaat tcaaaatagc gcacgtcttt gtgccagacg ggaacggttt
     1021 tttcggcgaa tccgatgccg tagagttttt tgatttgggc gaacagtccg tttaatactt
     1081 tgccgacggg gaagtatttt ttgacttcgg tttcgctgaa tgcgtatttg gcttcgcgca
     1141 gtttttcgcc ggcgtagctc aagtcccacg gctgcgggtc ggcgagaccg aggtgttcgc
     1201 gggcgaaggc tttgacttcg gcgaggtctt tttcggcgta gggtttggcg cggcgggcga
     1261 ggtcgtgcag gaagtttaaa acctgttcgg gcgtgtccgc cattttggtt gccagcgaca
     1321 attcggcgta atttttaaag ccgagcagtt tggcggtttt caatgcgttt tcgagcgtgc
     1381 ggtcgatgtt ggcggtgttg tcgaatttgc cgtcgtttga aagttcgctg gcacgggtaa
     1441 cgtaggcgcg gtagatttgt tcgcgcagtt cgcggttgcc ggcgtattgg atgacggcaa
     1501 ggtagtgcgg aatctgcaag ccgattttgt aacctgtttt gccttcgctt tgcgcggcgg
     1561 cggcaaacat cgcgagcgcg tcttcgggaa tgccggcaag cggtgcggca tcgtcaaagt
     1621 aaatgccgaa cgcgtcggtc gcgtctagga cgttttggga gaatttggcg gaaagttgcg
     1681 cgccttcggt ttgcagtttt gccagttctg cctgccgttc gggcggcagt tccgcgccgc
     1741 tcaatacgaa atcgcgcagg tcgtgatcga gcttggtttt ttgtgcgggg gaaagcgttg
     1801 caaattcggg ggaatttttg atggttttga agcggttgta cagttcgatg tcttgtccga
     1861 tttcggtgaa gaagacggtg atttcaggca tcagttcgtt atagacggcg cgcagttcgg
     1921 gcgtgtcgac gacggagttg agatgggaca cgacgcccca aatcctgccg acgcgttcgg
     1981 tgatgccggt cagacgctcg acggtgttcg cccagccggt gtgcgtttgc gctttgacgg
     2041 cggcgatttg tccgcgcgct tcggcgatgg cggtttggac ggcgggtttg atgtcttcgg
     2101 ttttgatttg attaaaacgg ggttcttcgc ccaagtggag cagtgcgttg tcaatcataa
     2161 gatgggtttc ctttgcgtgt tgtgcttcag acggcatatc ggcaatatgc cgtctgaagc
     2221 cagaaatggg gccggccggg gaaagttaaa gtgtttcgga gccggcggga tgatttaaca
     2281 ggcggtattg tacgcgcaaa aattcgtcca taaccgcctg ttggatttcc agccgctttg
     2341 aaacggggga ggcgaagcgg acgatgatgc ggtatgcctt gtcgtcgtac ggtacgcggg
     2401 taacgcgcgg cctggcggcg ggcgtgataa acagtttttc cgcctgcacg ttttccaaat
     2461 accgctgaat ggcggggatg tagggcgcgc acaagggctc gagtacggct ttcagacggc
     2521 atacggcttc atccgaatcc aaatggatgg gaacggggat ttcgaccgta tggatgacat
     2581 agtcgcccaa aatattgtcg cggcgcacgg ggtggctcaa caacaggctg ttggggaaag
     2641 aaacggtggt tcccgcaagc tgtccgacca aggggttcgg accgacctgc atcatcagcg
     2701 tgttcaacag attgatgtcg accacgcgcc cgcgcaggcc gttgatttcg atatagtcgc
     2761 cgaccgagta ttgctgggtg gcagacctta aaatactgcc cgacagacac ataatcagtt
     2821 cttttgtcgc cacgacgacc gccgccgcca ccgcaaacat cgacaaagcc agcgtttgaa
     2881 tttgcgccga ccagataaat gccagcgaaa acagcaccaa aagcagcgtt atattgcggc
     2941 tggcaaccaa aaaccgccgc ttgctttcga tgccgaaatc cggatgccgt ctgaagtgga
     3001 tattcaacag aagggcgcgc gccagcagca aagccgcgac cgccgccacg gattcggccg
     3061 cctccgcgcg tatcgggacg gcatcgggcc aagtgttcaa catattccat atttccattt
     3121 ccgcgcccct gtccgaaaag tatagggtgt agattttagt ggcaaaaaaa cgcttttgcc
     3181 actttgccgg caaattccgc gcccgtaccg gccgcaaatg cgccgcacgc cctttcagac
     3241 ggcatccgga tgcggataac gctgaatcag gcggcgaaat cggctataat acacaattat
     3301 atgcgccgtc ccgcccgctt tttccacaag gtgcggactg ttttggcggc atcgcaaaat
     3361 cttttcaaaa tccggcaaaa aatatgactg aacaaaaaca cgaagaatac ggcgccgaca
     3421 gcatccaggt gctcgaaggc ttggaagcgg tacgcaaacg ccccggcatg tacatcggcg
     3481 acacgcagga cggcagcggg ctgcaccata tggtgtttga agtattggac aacgccatcg
     3541 acgaagcact cgccggacat tgcgacaaaa tcacggtaac gatacacgcc gaccattccg
     3601 tcagcgtcgc cgacaacggg cgcggtatgc ccaccggcat ccacccgaaa gaagggcgtt
     3661 ccgccgccga agtcatcatg accgtcttgc acgcgggcgg caaattcgac aacaacagct
     3721 acaaaatctc cggcggcctg cacggcgtgg gcgtatccgt cgtcaacgcg ctgtccgact
     3781 gggtaacgct gaccatctac cgcgacggca aagaacactt cgtccgcttc gtacgcggcg
     3841 aaaccgaaga gccgctgaaa attgtcggcg attccgacaa aaaaggcacg accgtgcgct
     3901 tcctcgccgg cacggaaacc ttcggcaata tcgaatacag cttcgacatc ctcgccaaac
     3961 gtattcgcga actttcgttc ctaaacaacg gcgtggacat cgaattgacc gacgagcgcg
     4021 acggcaagca cgaaagcttc gccctttccg gcggcgtggc gggcttcgtg caatacatga
     4081 accgcaaaaa aacgcccttg cacgaaaaaa tcttctatgc gttcggcgag aaagacggca
     4141 tgagcgtcga atgcgcaatg caatggaacg acagctatca ggaaagcgtg cagtgcttca
     4201 ccaacaacat ccctcagcgc gacggcggta cgcacctgac cgcgctgcgc caagtgatga
     4261 cgcgcaccat caacagctac atcgaagcta acgaagtcgc caaaaaagcc aaagtggaaa
     4321 ccgccggcga cgatatgcgc gaaggtttga cctgcgtgtt gtccgtcaaa ctgcccgacc
     4381 ccaaattctc atcccaaacc aaagacaaac tggtttccgg cgaaatcggc cccgttgtca
     4441 acgaagtcat caaccaagca ctaaccgact tcctcgaaga aaatccgaac gaagccaaaa
     4501 tcatcaccgg caaaatcgtc gatgccgccc gcgcacgcga agccgcccgc aaagcccgcg
     4561 aaatcacccg ccgcaaaggc gtgatggacg gcttgggact gcccggcaaa ctcgccgact
     4621 gccaagaaaa agaccctgcc ctgtctgaac tctacctcgt cgagggcgac tccgcaggcg
     4681 gttccgccat gcagggccgc gaccgcaaat tccaagcgat tttgccgctc aaaggtaaaa
     4741 ttttgaacgt cgaaaaagca cgttttgaaa aaatgctcgc cagccaagag ctcgccaccc
     4801 tgattaccgc gctgggtgca ggcatcggca aagaagagtt caaccctgaa aaactacgct
     4861 accaccgcat catcatcatg accgatgccg acgtggacgg tgcgcacatc cgcaccctgc
     4921 tcctgacctt cttctaccgc caaatgcccg aactggtcga gcgcggctac atttacatcg
     4981 cccagccgcc gctctacaaa gccaaatacg gcaagcagga gcgttacctc aaagacgaac
     5041 tggaaaaaga ccaatggctg ctcggccttg ccttggaaaa agccaaaatc gtttcagacg
     5101 gccgcaccat cgaaggcgca gaacttgccg acaccgccaa acaattcttg ttggcgaaaa
     5161 ccgtcatcga acaggaaagc cgcttcgtgg acgaactcgt cctgcgtgcc atgctgcacg
     5221 cgtcgcccat tgatttgacg tcgtctgaaa acgccgataa agccgttgcc gaactttccg
     5281 gtttgcttga cgaaaaagaa gccgccctcg aacgcatcga aggtcatgaa ggacaccagt
     5341 tcatcaaaat cacgcgcaag ctgcacggca acgtcatggt cagctacatc gaacccaagt
     5401 tcctcaacag caaagcctac caaaccctca cccaaaccgc cgccgcgctc aaaggcttgg
     5461 tcggcgaggg cgccaagctc tacaaaggcg agaacgagta cgacgcggac agctttgaaa
     5521 ccgctttgga catcttgatg agcgttgccc aaaaaggtat gtccatccaa cgatacaaag
     5581 gtttgggcga gatgaacccc gagcagcttt gggaaaccac gatggatccc accgtgcgcc
     5641 gcctgttgaa agtgcgcatc gaagatgcca ttgccgccga cgaagtgttc gttaccctga
     5701 tgggcgacga ggtcgaaccg cgccgcgcct ttatcgaaaa caatgcgctg attgcgcaaa
     5761 atatcgacgc ataagtgccg ttttaaaaaa ggagacgggc agcttgccgc gtctcctttt
     5821 ggtttgtcaa acggaagcca tgccgtctga aaccgccttt cggagcaaaa ccatgatcag
     5881 catttttgat attttcaaaa tcggtatcgg cccttccagt tcgcatacgg tcggcccgat
     5941 gaaggccgcc gccgcctttg ccgcaggctt ggacgcgcaa accgcccgca tcgccatcga
     6001 catttacggc tcgcttgccc tgaccggata cggacacggc acatttgacg cgctgatgct
     6061 cggtttggaa ggcagcctgc cgcacgacat cccgcttgcc ggcattcccg aacgcctcgg
     6121 acgcatccgc acgcagcaca tcctccggct caacgggcaa gaaatccgct tcatccccga
     6181 ccgcgacctg aacatacgcg gcaatcaagt gctgcccaaa caccccaacg gcctgcgatt
     6241 taccgcctat gcttcagacg gcacggtatt gaaggaacag gtttattatt cggtcggcgg
     6301 cggctttgtc gttaccgaag aagacttcga gtggcagtcg gaaacggaaa aagccgttcc
     6361 ttatccttac aacagttgtg ccgggctgct tgcccgatgc cgtctgaacc ggctcgacat
     6421 ctccgaagtc gtgttggcaa acgaagccgc gcttgccgga tgcggcgaag ccgaaatccg
     6481 ccgccgggtt gccggtgttg ccgagattat ggaagagtgc atcaaacgcg gcttgggtgc
     6541 ggacggcgaa ctgcccggcg gattgaacgt ccgccgccgc gccccgcagc ttgccgccaa
     6601 actcaaagtc ctgcgcgaaa ccgaaatcgt caacacccag ctctggccga tggtgtacgc
     6661 catggcggtc aacgaagaaa acgccgccgg cggacgcgtc gttaccgccc cgaccaacgg
     6721 cgcggcaggc atcattcccg ccgtattgca ttatttccgc aagttcaacc cccacgccac
     6781 acaggaacgt gtcgaaaact tcctgctcac cgcaggcgca atcggcatcc tctacaaaac
     6841 caacgcctcc atttccggcg cggatgtcgg ttgtcagggc gaagtcggcg tagcgtgttc
     6901 aatggcggcg ggtgcatacg ccgaagtcat cggcggcacg cccaaacaag tggaaaacgc
     6961 cgccgaaatg gcaatggaac accatttggg cttaacttgc gaccccgtcg gcgggttggt
     7021 gcaaatcccc tgcatcgaac gcaacggcat cgccgccgaa aaagccctca aactcggcac
     7081 gctcgcgctc ttggaagacg gcacggacaa aaaagtctcg ctcgacgaag tcatccgaac
     7141 catgctgcaa accggccggg acatgaaggc gacctacaaa gaaacctcgc ttgccggact
     7201 cgccgccacg ctccggaaaa aagccgtccc cgtatccgtg cgcgtggtcg agtgctgagg
     7261 atggacagaa acgaaaatgc cgtctgaaaa cagtttttca gacggcattt catttatttt
     7321 ttgcgggttt accgcttcaa cgccaacgtc aacacgcccg ccgtaaccag ccccaagcct
     7381 atccattcct gcgtgttcgg gcgttcgtcc aagaaaacca ccgccatcag gaatgcaaac
     7441 cggcgttgtt tgcagcatcc ggcaagaatt tttatagtgg attaacaaaa atcaggacaa
     7501 ggcgacgaag ccgcaggcag tacaaatagt acggaaccga ttcacttggt gcttcagcac
     7561 cttagggaat cgttctcttt gagctaaggc gaggcaacgc cgtaccggtt tttgttaatc
     7621 cactataaaa ggtcttttct aaaatatgca gatattcgaa tgtcgaattt gccttatgat
     7681 tacggttttc tgtcttatcc gccttaaaac gccggtattc cgtttgaacc aaattatatt
     7741 tgccgaaaca ctcaaaaatc tcccttacct gcccgacaga catcaagact tcgttgttgt
     7801 aactgagaaa aatatattga aagtccgcat ccctaatcaa agcctcaaat accggaacca
     7861 catccgattt tgatcaagtc cgcgctaaaa cacatcgtac agcgcaaaac cggatgggaa
     7921 tggcaaaatc cggaaacaaa catttttcaa agcgtacaaa caaaaaatac caaccggagg
     7981 ttggtatttc ttaact
//
LOCUS       DAWTHD010000098         7865 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWTHD010000098 DAWTHD010000000
VERSION     DAWTHD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.098"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            173..922
                     /locus_tag="WM704_001753"
     CDS             173..922
                     /locus_tag="WM704_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFA9341119.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWGEDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            1013..3376
                     /locus_tag="WM704_001754"
     CDS             1013..3376
                     /locus_tag="WM704_001754"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFA9341120.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            3450..3752
                     /locus_tag="WM704_001755"
     CDS             3450..3752
                     /locus_tag="WM704_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFA9341121.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            3736..4044
                     /locus_tag="WM704_001756"
     CDS             3736..4044
                     /locus_tag="WM704_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341122.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            4220..4705
                     /gene="fxsA"
                     /locus_tag="WM704_001757"
     CDS             4220..4705
                     /gene="fxsA"
                     /locus_tag="WM704_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HFA9341123.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            5339..6640
                     /gene="bioA"
                     /locus_tag="WM704_001758"
     CDS             5339..6640
                     /gene="bioA"
                     /locus_tag="WM704_001758"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFA9341124.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRAFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            6637..7284
                     /gene="bioD"
                     /locus_tag="WM704_001759"
     CDS             6637..7284
                     /gene="bioD"
                     /locus_tag="WM704_001759"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HFA9341125.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            7299..7775
                     /locus_tag="WM704_001760"
     CDS             7299..7775
                     /locus_tag="WM704_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFA9341126.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
BASE COUNT         1985 a         1688 c         2204 g         1988 t
ORIGIN      
        1 actatttttc ttcagaaata gacgactggt tattggcaag cgagaaggag ttaaaagatt
       61 atgatgtata cctatacaaa ttagtactag atggacagca atatgttcag ggaataagaa
      121 aatctgtatt gtacgatttc cttatggaaa atcagaaaga tagctgataa aaatgaaaaa
      181 atggactaga gatgaaaccc tggttgccct ttatctttat tacatcattc cttttcaaaa
      241 ggttagtaag gacaatcctg ttattcaaga atatgctgaa atactgggca ggacaccgtc
      301 cgcactcggt atgaagatag gaaatttggg tcgtttagat cctacgctga aagtcaaaaa
      361 tatttcggga ttaagtaatg gcagcaaaat ggatgttgtc gtatggaatg agttttcagg
      421 agattgggag caacttaata aagaatttga gggagtcatt tcccaatacc aatcaaatga
      481 tgagaatagt aatattgaaa tagaatcacc tgagattcca aagggaaggg aaaggtttgc
      541 aagaatatca gtacgcgtca atcagggctt tttccgttcc agtgttttgg cagcatacaa
      601 taatcaatgt tgtattactg gattaaaaca acctgaattg ttagttgcta gtcatatcaa
      661 accctggggg gaagataagg ataaccgttt aaacccaagg aatgggctat gtttgaatgc
      721 gttacatgac aaagcttttg atagggggtt gttaggaata gatgaaaatt tcaaaattat
      781 attttctcca ctactggcaa aaacagaagg atttgatgat ttatttaaac catatgaaaa
      841 tcggatgata agactgcctg aaagactaaa cccaagtctg gaatttttaa aatttcacag
      901 agaaaatatt tttcagagtt gagtaaatat caacattaac cagaattcta aaacagactg
      961 tctgaacgaa aattgataaa acaaaagtta attaattgat tgagaacata acatgcaatt
     1021 ctcctactca tggctgaaaa cccaagccaa tcctgatctt tctgccgata aactggaaca
     1081 tcttttgacc atggccggtt tggaagtgga agaaatcgat actgccgccc ctgctttcag
     1141 tggcgtggtt gttgccgaag taaaatctgt tgaaaaacat cctgatgcag accgtttgaa
     1201 cgttactcaa gttgatgccg gtacgggcga gttggttcag attgtttgtg gtgcgccgaa
     1261 tgtcaagccg ggcattaaag tgccgtgttc gttgccggtt gccgttttgc cgggcaattt
     1321 taaaattaag ccgactaaaa tgcgcggcgt accatcaaac ggtatgctgt gttcgaccaa
     1381 tgaactgggt ttgcctgatg atggtgtaga cggcctgcac attctgcctg aagatgcgcc
     1441 tgtcggtacc aacatccgcg aatacttgga tttggacgat atgctgttta cgttgaaaat
     1501 tacgcctaac cgcgctgatt gcttgagtgt taagggcatt gcgcgtgagg tttctgcgtt
     1561 gactcaatgt gcgtttacgc ctgttgaaat tcagacggcc cctatcggca gtgagaaaaa
     1621 acaggctgtc cgtattgatg caccggctga ctgcggccgt tttatcagcc gcgttattga
     1681 aaatgtcaac gcgaaagcgg ctactcctga ttggatgaaa caacgtttgg aacgcagcgg
     1741 tatccgcagc atttccgcat tggtggacat tggcaactat gtgatgctgg aaatcggtca
     1801 gcctatgcat gttttcgatg ctgataagct gtcgggcagt ttgattgtcc gccgtgctca
     1861 aaatggcgag actttggcgt gtctgaatga gaagacggtt actttggctg acaatacact
     1921 ggtggtcgct gatgaaaaag gtgcgttgag cttggccggc ttgatgggtg gcgcggcgag
     1981 cgcggtttca gacggcacgc aaaatatcgt gctggaagcg gcttggtttg agcccgaaat
     2041 catcgtcggc aaatcgcgcc aatacggttt cggttcggat tcgtcgttcc gcttcgagcg
     2101 cggcgtggat taccgtttgc aggcggatgc cattgaacgc gccaccgaat tggttttgca
     2161 gatttgcggc ggtgcggcag gcgagatggt ggaagcacaa ggtaaattgc ccgaagccaa
     2221 gcaggtcgga ttgcgtttgg gtcgtctgaa aaccgtgttg ggcgtggaca ttcctgccga
     2281 acaggtggaa accattttgc aacacttggg tctgcagcct gagaaaacgg cggaaggctt
     2341 ccgcattacc gcgccgagct tccgtttcga catcgaaatt gaggctgatt tgattgaaga
     2401 aatcggacgc gtttacggct atgaaaacat ccccgacgat tacacgtcag gccgtctgaa
     2461 aatgtcggaa ctgcccgaaa cacgccgtcc gcgttttgcc gtttacaacg aaatggcggc
     2521 gcgcggctac cgcgaagtga tcagctatgc cttcgttgac gagcagtggg aactcgattt
     2581 tgccgccaac gccgccccca tccgcctgca aaacccgctg gcggcgcaat atgccgtgat
     2641 gcgttctacg ttgattggcg gcttagtgga aattttgcaa aacaacctga accgcaagca
     2701 aaaccgcgtg cgcgtgtttg aaatcgcctg cgtgttcggc aaaggttcag acggccggtt
     2761 tgtccaaaac gaacgcatcg gcggtctgtg gtacggcgcg gcgatgcccg aacaatgggg
     2821 tgagaaaacg cgcaatgcgg atttttacga catcaaggcg gacgtggaaa atctgttgaa
     2881 aaacaaagcg gtcgagttcg ttaaaaccgg acatcccgcc ctgcatcccg gacgtgccgc
     2941 caatatcgtt tcagacggca aagtcatcgg ctttgtcggc gaactgcatc cgaaatggct
     3001 gcaaaaatac gacctgccgc aagcgccgct ggtatttgaa atcgatatgg cggctgtgtt
     3061 ggaatgcggg aaaacgcgct atcgggccgt atcgaaattc cagccggtgc gccgcgattt
     3121 ggcgtttgtg atgccggaag ttatgaatca tgatgattta ctgcttgtct tgaaaggcgc
     3181 agcaaacaag ttggtacagg aaatcagcgt gtttgacgtg tatcgcggca cgggtctgcc
     3241 cgaagggatg aagagcgtgg cggttaaagt gattttgcag gatatggaaa acacgctgac
     3301 ggatgaggcg gtcgagccgc ttattggaaa actgattggc gtggcaacgg aggcaggggc
     3361 gcggcttcgc agttaaaaaa taaatattcg cttgaatttt aaataaaaat tggtaataat
     3421 ccacaactgt tacaacagaa ggtaatcata tgacactaac taaagcagaa ctggccgata
     3481 ttttggtgga taaagtcagc aacgtcacca aaaacgatgc caaagaaatt gtcgaactct
     3541 tttttgaaga aatccgcagc actttggcaa gcggcgaaga aatcaaaatt tccggtttcg
     3601 gaaatttcca gttgcgcgac aagccgcaac gcccgggccg taatccgaaa accggcgagg
     3661 aagtgccgat taccgcccgc cgcgtggtaa ctttccatgc cagccagaaa ctcaaaggca
     3721 tggtggaaca ctactatgac aaacaacgtt gatttgacga ttcctgcaaa acgctatttc
     3781 acgctggacg agttgtgcgg actgttgcaa atcagcccct atggttttgc gcaatggcaa
     3841 catgatcacg gtgtggttgt cggttacggc ggagaacgct acacccgttt ggatgtggtg
     3901 aaactgttga aactgaagag cacgtttgca ccgtatgcag aaggtgcgga atcgggttcg
     3961 gacggcaacc gtccggttac gcttcaggaa atcggagacg gtctgaaaga cctgttggag
     4021 gatttggata aggagttgtg ctgatttgag accggttgca ggtatgcagc cggttttgtt
     4081 ttacacgcta aaaataatta ttgcgtgatt tcacattgtt tcggcttgaa gcacatggtt
     4141 ttgtaatcat ttacaggcag ctcgcttgga atcctgttcg ggcggtttgc tgtttactta
     4201 aatataagga tgacggtcaa tgagattttt cggtatcggt tttttggtgc tgctgttttt
     4261 ggaaattatg tcgattgtgt gggttgccga ttggctgggc ggcggttgga cgctgtttct
     4321 aatggcggca acctttgccg ccggtgtgct gatgctcagg catacggggc tgtccggtct
     4381 tttattggct ggcgcggcgg taaaaagtag tgggaaggta tctgtttatc agatgttgtg
     4441 gcctatccgt tatacggtgg cggcggtgtg tctgatgagt ccgggattcg tatcctcggt
     4501 gttggcggta ttgctgctgc tgccgtttaa gggaggggca gtgttgcagg caggaggtgc
     4561 ggaaaatttt ttcaacatga accaatcggg cagaaaagag ggatttttcc acgatgacga
     4621 tattatcgag ggagaatata cggttgaaga gccttacggc ggcaatcgtt cccgaaacgc
     4681 catcgaacac aaaaaagacg aataaatatg aatggaatgc cgtctgaagg ttcagacggc
     4741 atttttccgg tttgaaaata tagtggatta acaaaaacca gtacggcaag gcgaggcaac
     4801 gccgtactgg tttttgttaa tccactataa atttaatcca ctataaaata gggctgtaac
     4861 cttcaatcgg aatttgttgc ctgcgggata tacggtatga gtgtttggga tatatgggac
     4921 aggatggtgg aaatctatca taagtataag aagccgtgcc tggttttagc agtggatttt
     4981 gtgatgggta tggtattcat agagccgaat gaggagccgt gcatcggcag atgctatgcg
     5041 cctatgccgg agtcccctga ttttgccaac gctgttgcga tggctgttgc tatgatctgt
     5101 atcgtattta tctatgattg attgaagcgt tgggcggagg ctgtgtgaaa cggtattggg
     5161 cgttgggtcg tctgattcca atcaggcttg aggaatgcga aacggtgtgc gcttatgttg
     5221 cggacgattt gtttcgcggt ttttcgcccg aaacggatgg aaaggtgtgg gaagcggtct
     5281 gtcggagtag aatacgcttt ttgcgtttga atgcagtaat aagaaaagag agaaacttat
     5341 gccgtctgaa catcaacaca catcatcatt acttaatttc gaccgtaccc atctgctcca
     5401 tccctatact tccatgaccg atccgcttcc cgtttatcct gtcaaacgtg cagaaggggt
     5461 gtttatcgaa ttggcggacg gcacgcggct gattgacggg atgtcgtctt ggtggtgtgc
     5521 gatacacggt tacaatcatc ccgttttgaa tcaggcggtt gagaatcaga tgaaacaaat
     5581 ggcgcacgtg atgttcggcg gcctgacgca cgagccggcg gtggagctgg gcaagttgtt
     5641 ggtcgggatt ttgccgcagg ggctggaccg tattttttat gcggattcgg gttcggtttc
     5701 ggtagaagtc gcgttgaaaa tggcggtgca ataccagcag gcgcggggtc tgacggcgaa
     5761 gcagaatatt gcgacggtac ggcgcgggta tcacggcgat acttggaacg cgatgtccgt
     5821 ttgtgatccg gaaacgggga tgcaccatat tttcggcagc gcgttgccgc agcgttattt
     5881 tgtcgataat ccgaaaaacc gtttcgacga tgaatgggac ggggcggatt tgcagcctgt
     5941 ccgcgccttg tttgaagcgc atcatgtgga tattgccgcc tttattttag aaccggtcgt
     6001 gcaaggcgcg ggcggcatgt atttttatca tccgcagtat cttcgcggct tgcgcgattt
     6061 gtgcgacgaa tttgatatcg tgttgatttt tgacgaaatc gccaccggat tcgggcgcac
     6121 gggcaagatg tttgcctgcg aacacgcgga ggtcgtgccg gatattatgt gtatcggcaa
     6181 gggtttgagc ggcggctata tgacgctggc ggcggcaatc acttcgcaaa aagttaccga
     6241 aacgatttca cgcggcgaag cgggcgtgtt tatgcacggc ccgacattta tggcgaaccc
     6301 gctggcgtgt gccgttgcct gtgcttcggt caagctgctt ttatcccaag actggcaggc
     6361 aaatatccgc cgtatcgaaa gcatcttaaa aggccgtctg aaagccgcgt gggacattcg
     6421 cggcgtgaaa gacgtgcgcg ttttgggtgc tatcggcgtg atagagttgg aaaaaggcgt
     6481 ggatatggcg cgttttcaag cggattgtgt ggcgcagggc atttgggtac gcgcgttcgg
     6541 caggctggtg tatctgatgc cgccctatat catttcagac ggcattttga ccaaacttgc
     6601 cgataaaacc gtgcaaatct tgaaggaaca cagcaaatga aaggcgttta cttcgtcagc
     6661 ggtatagaca cggacatcgg caaaaccgtc gccaccggta tgttggcaaa acaattgttg
     6721 cagcagggca aaagcgtgat tacgcaaaag cccgtgcaaa ccggttgcca agacatcgcc
     6781 gaagacatcg ccgtccaccg taaaatcatg ggcataccca tgcaggaagc tgatgaacag
     6841 cgtctgacta tgcctgaaat cttcagccat cccgcctcgc cccaccttgc cgcccgactg
     6901 gacggcaggg gtttggactt ggacaaaatc cgcaccgcca cacaagaatt ggcggcgcag
     6961 tacgaagtcg ttttggtcga aggcgcgggc gggttgatgg taccactgac ggaaaagctg
     7021 ttaaccattg atcatattca gcaacaggct tatcccgtca tccttgttac cagcggacgg
     7081 ctgggaagca ttaatcacac cctgctcagt ttcgtcgtgc tcaaacaata cggcattcgc
     7141 ctgcacagcc tcgtgtttaa ccacatccac gacagccgcg acgcacacgt cgcccaagac
     7201 agcctgaact atctgcaatg ccgtctgaaa gccgattttc ccgaagcgga atggatggag
     7261 ttggcaaaaa caggcgcggt ataaagattg ggaaagatat ggaacaccta tttgggaaat
     7321 ggctgcccga cttgcccgcc cccgtttcag acggcatcga cctgccgatg agccggttat
     7381 tgaaagcccg gtcgctgacc gccgcattgt gcgccttgcc gcatacattt tcggtggaac
     7441 tgctgaaact gggcgaagcg gaaacggaat acggacggcg gagggtgcgc gatgtgcttc
     7501 tgaaactgga cggcactgct gttgtgcagg cacgaagcgc gtgcagcgtc ggttcggcgt
     7561 tttggcaaaa cattttggac tgcggcacac gccctttggg cgagcgtctg tttcaagccg
     7621 atttggaagg ggcgcgttcg gcgtttgagt ttgccgtttc cagtgaagga tgcggacggt
     7681 actttgccgc gcggcgttct cggttttccc atcaaggcga ggaaatgctg ctgaccgagt
     7741 attttctgcc cgaactgaaa cgctttatcg gataaaatac cgttttttca agctgtgcgg
     7801 caacctgcat ctaaaccaca ttttaatata agagactcca atttagatac aggacatgtc
     7861 gatgg
//
LOCUS       DAWTHD010000099         7863 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWTHD010000099 DAWTHD010000000
VERSION     DAWTHD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.099"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..3360
                     /locus_tag="WM704_001761"
     CDS             <1..3360
                     /locus_tag="WM704_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9341127.1"
                     /translation="AFADTPNNTEQQKELNTIVVHGKRSADQKGADDVYYKNVSNAYV
                     GKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAITPNIRGITGKGRIPVTIDGTEQT
                     IDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRGVKSGVGGAMSIRTIEAGDIVPEG
                     QKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLPLGATADGAGGGSDPITGQQSPQA
                     LVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTELSDGLAAYSYRNKGNYFAGRKGA
                     EGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMARIYHPGVEVLNSNTETKTLLLKN
                     NWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNMEEHNETNRPKELSPQAQSIDSTI
                     RTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRHQSGGMDLSSARPDPFYDVWYWCT
                     QRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLAMTPNDNGQFRVISGAEQKTRVSR
                     TGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIVNSKDLFNLAGMVTGMTKLAGPRG
                     GERREWGTNLVFDWQAADRLKISAGIRYHNFRGFDTALAEGRARRDPRYQAGDGTQHY
                     ADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGDAAAIAAAVQAEQAHGARYHLLKY
                     YDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQPLYRVRPVYVPFVNGKLDSGALPQ
                     HFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPIDCIRSDAASCLAHNLSGKVTNFQV
                     GYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAHAWAPTIAVSYDLTDNSRLFVRYA
                     QMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPERSRSWEIGYSFNFAPYWSRLRAGD
                     MRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEWQSRIDTGRFFAALGATYRLKQQM
                     CDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQPKYSINLDAGVRLLGEKLELGLR
                     GIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRSSLTWDVYGRYRLHKNLNVNLGIT
                     NLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            3575..4558
                     /locus_tag="WM704_001762"
     CDS             3575..4558
                     /locus_tag="WM704_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341128.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            4829..6319
                     /locus_tag="WM704_001763"
     CDS             4829..6319
                     /locus_tag="WM704_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9341129.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(6387..7784)
                     /gene="aspA"
                     /locus_tag="WM704_001764"
     CDS             complement(6387..7784)
                     /gene="aspA"
                     /locus_tag="WM704_001764"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFA9341130.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         1866 a         2287 c         2134 g         1576 t
ORIGIN      
        1 gcttttgcag acaccccgaa caataccgaa cagcagaaag aactgaatac catagtcgtc
       61 catggcaaac gcagtgccga ccaaaaaggc gcggacgacg tgtattacaa aaacgtctcc
      121 aacgcctacg tcggcaaaga atacctcgaa cgctaccgcg tccaatccgc cggcgacgta
      181 ctcaaaggcc taaacggcgt gtacaacatg aacacccgca ccgccggcgg cgccatcacg
      241 cccaacatac gcggtatcac cggcaagggc cgcatcccgg ttaccataga cggcaccgaa
      301 caaaccatcg acgtatggat gaacaactac ggcgtgggcg accgcaacta cctcgacccc
      361 gccctgttcc gcagcatcgc ggtggaaaaa agccccgccc tcacgcgcgg cgtgaaatcg
      421 ggcgtcggcg gcgcgatgtc catccgcacc atcgaagccg gcgacatcgt gcccgaaggg
      481 cagaaattcg gcttccagct gaaaaccgag tttgccaaca acagaacccg cccggccaac
      541 aacctcaacc aatggctggg ctgggaagac taccgcaccc tgccgctcgg cgccaccgcc
      601 gacggcgcgg gcggcggttc tgacccgatc accggacagc agagcccgca ggcgttggtg
      661 gtagacaact ttaccccgcc gcagcacaaa agcggccgcg acaactggcg cttcggcggc
      721 gaccgcagtt atatggccgc cgccgccttc aaaaccgaac tttcagacgg ccttgccgcc
      781 tacagctacc gcaacaaagg caactacttc gccggtcgca agggtgcgga aggctacctg
      841 aataatccgg tatacgacct gcaaaaatgc tacgacgaag gcggcagcga tttcaactgc
      901 aaaaattccg ccaccttcat ccccaatatg gcgcgcatct accaccccgg ggtggaagtg
      961 ctcaacagca acaccgaaac caaaaccctg ctgctgaaaa acaactggca cctgcccggc
     1021 agccatcggc tcggctggca atatatgcgc accgacgtgc gcttcggaga gatcaacccc
     1081 ttccacacca cctatgtgat gaatatggaa gagcacaacg agaccaaccg ccccaaagaa
     1141 ctgtcgccac aggcgcagag catcgattcc accatccgca ccgacaccta caaactcggc
     1201 tgggcctgga agccggaaaa cagccgctgg gtcgatctgc aggccaacct gtggcgcatc
     1261 aaaaccaaca gcacccgcca ccagagtggc ggcatggatt tgtcttccgc ccgccccgat
     1321 cccttctacg acgtttggta ttggtgtacc caacgcggcc ggataccgcc cgagcacgtc
     1381 gacaactaca gcagctgcaa cgacctgatg aatgatttcg gcgtcaacgg cctgaccaaa
     1441 gaacaggtgt tggccatgac cccgaacgac aacggccaat tccgcgtgat ttccggcgcc
     1501 gagcagaaaa cccgcgtcag ccgcaccggt ttcgatatca gcaaccgctt ccgcctcaga
     1561 gaccgcctga gcatgacctt ggccgccgat tatcagaaag agaaactggc ggaggaagtg
     1621 gaaatcgtca attcaaaaga cctgttcaat ttggccggca tggtaaccgg catgaccaag
     1681 ctggccgggc ccaggggcgg cgagcggcgc gaatggggca ccaatttggt gttcgactgg
     1741 caggccgccg accgcctgaa aatctccgcc ggcatccgct accacaactt ccggggcttc
     1801 gataccgccc tggccgaagg gcgtgcccgc cgtgacccgc gctatcaggc aggcgatgga
     1861 acccagcatt atgccgatgg tgcctatctg ccgtatttcg aactggcagg cgatcaggag
     1921 cggcgtgact ggaatgtggt ggccgaacag cttcggcagg cgtatcagtc aggcgatgct
     1981 gccgccattg ccgcggcagt acaggcagaa caggcgcacg gcgcccgtta ccatttgctg
     2041 aaatactatg acggctcgta tcgtaccgat ggttatgtgt atatcggcaa cgatggccgt
     2101 gcctattttg acggacggtt cggcggggtt aacgaggcac agaaccaacc gctctatcgg
     2161 gtgcgccccg tttatgtgcc gtttgtcaac ggcaagctgg acagcggcgc attgccccag
     2221 cattttcatg atttcgtcag caattatcag gaaaaagtga ccaatccgca ggggctgcac
     2281 gggacgcatt atcgatattg ggcaggcgtg gggaatccta tcgactgtat aagaagtgat
     2341 gcggcctctt gccttgctca taacctatcg ggaaaagtaa ccaattttca agtcggctat
     2401 cccgagtacg gtgatgatac cggtctcggt ctcaaaatca tcggtcggca ctataccgag
     2461 gaacagtatt gggcgcagcc caaaccgatc cgtgcccatg cctgggcgcc gaccatcgcc
     2521 gtcagctacg atctgaccga caacagccgc ctgttcgtac gctatgcgca gatgagccgc
     2581 ttccccagcg tgtatgaggt gggcagtttt tataacgacg tggcctatgc cggtatgccg
     2641 aaggccccaa atttccgctt caagccggaa cgcagccgca gctgggaaat cggctatagc
     2701 ttcaattttg ccccgtattg gagccggctg cgtgccggcg atatgcgcct gacctactac
     2761 cgcaaccgta tccaaaatgt gatcgaaacc acagattatt tccgcaccgt gcaatacgac
     2821 cgcaaggaca ccgccgggct ggaatggcag agccgcatcg acaccggccg cttctttgcc
     2881 gcgctcggtg ccacctaccg cctgaaacag cagatgtgtg accgcgatac cgctttcgat
     2941 tacgaccctt atggtgccaa aggcgtgccg atctgcatcg aaggcggcta cggcagtacg
     3001 cgcggctatc aggccttgca gccgaaatat tcaataaacc tggatgccgg ggtgcgcctg
     3061 ttgggcgaga aactggagct cggcctgcgc ggcatctacc acagccgggt caacaccaag
     3121 caatacgacc aactgctgca aaaggatttg ggcatcatct tcgataccac cggcaagccc
     3181 caccattggc ggtcatcgct gacttgggat gtttatggcc gctaccggct gcacaaaaac
     3241 ctcaacgtca acctcggcat caccaacctg accaaccgct actatctcga cccgatgtcc
     3301 aacgttgccg cccccgggcc gggccggacg gttaccttcg ggctgactgc caagttctag
     3361 ccgcagggcg gagtggggtt ttcaggtagc ctgcgggtat ggcgggaagg ctacctgaaa
     3421 acggattgga tataacaaca aacaattgaa cactccggac gcacaaaccc aaccggcccc
     3481 gcttatccga ggtaatggtt tttaccgttt tttccgccgg ttcggcggta ataaaacggt
     3541 ggcttttgtt taactcttaa ttaaggaaac gattatgtct atacgtaata aaaccctgtt
     3601 ggccgtcttg ctgtgcggcg tttttggtgt ggctgcggct gctccggctg gtggttccag
     3661 ccaaaggaaa agccaagatc caaatagtga cccgacgaca gttcgccccg gcacctctga
     3721 caagcaacct ttcggtgccg gtaaggccgg tttggcattc agtatcaata acggtggaag
     3781 ttggtcgaaa tccatcaatc tggatgatgg tccggtcaaa tatatccgtg cgaaatatca
     3841 ggcaccgatt aacggcaacg atccctatgc taatgttgat ggtacgcgca atctgcaata
     3901 tctgcgctta ggcgatgtga aaccggttat cggctggttc tacaacccaa cgttgggtca
     3961 ggtgtggtat gaaaaccacc acaacaacac tgaagtgtac agtgtgcgcc agatagccaa
     4021 cccggcaatc cccgccgcac ccaagttcgg cggcctggta atcgccaaag tgccgggcat
     4081 gggcggcagc aacggcaacg tcttcttcgg cgaatgggcg ccgcgtgccg gtaacccgca
     4141 gcaaatcagc accgacctga atatggcgga tggtaaacgc accgtttggt atgtgggcga
     4201 taatcccacc ggcaacacca ccggtgtgag aaccgccacc tacaatgtgc tgggtatcaa
     4261 taagcacacc ccggggcaga acgatttcta taccggtcag gtaaccgcca ccttcggttc
     4321 tggtataacc gggacgatgg caggcagaat cagccgcagt tccgatcacg tcgattttga
     4381 tcacgccaaa atcgataaca gaaatggtac ctttgccgat cccagtcaag gcatgaccgg
     4441 ccgcttctac ggcaaccagg cggcagccat ggccggctat accacacgtg gtaccggtaa
     4501 tgtcggcgat gacgtagctt tcggcggtag cgccaccgat attgagaccc gcaaataaca
     4561 gctgccatcc gatttggttt ggcaacggct aggctggatt taacccctga ggagtttcaa
     4621 gctgccgttt tatgccgact gccaagccat ataaatcatc cgtccgatac ggattgctgc
     4681 tcggtttcag gtagcccgtg ttcaacacag gctacctgaa atttgatatt tgtaaataaa
     4741 catcgcctat cccatacggt ttcggcctat cggacatttt gggacagcaa ttaaaacctg
     4801 cgttacttta tcttttcgga aggcaaccat gaacaaacca ctcagcctcg ccctcttctg
     4861 cctgcttctg cccatcaccg cccacgccga caaccccgcc gacgaacgcc gccgcctgct
     4921 ggacgagggc agccgccaaa cgcagcaata ccgcgaaagc ggttggctgg acacggaaca
     4981 ggcacgcggg gaagtagagg aaaacgacgg ctatatttcg ataggcggcg aaatctacca
     5041 agtcggcgat acggcggaag aactggaaag cgcaatttac cacgccctca atgcgcggca
     5101 gtggcacaag gtgcggcagt tcgccgcccg ctacgccaaa ctgccccggc acaagcccgc
     5161 gctgattcat ttggccgatg cgctgcaaaa acgggacgaa ggggatttcc gcgcggcggg
     5221 gaacagtttt cagacggcct tggaagccga acccgacaac ccgcgcctgc tgctcgaagc
     5281 ggggcggttt tatgccgaag acaaccaaaa caaagagtct gccgccgcgt ttgagaaagt
     5341 tctgaaaacg gacattcccg cagaaacccg ccccatcgtg gaaaactacc tgtccgaact
     5401 gggcaaacgc cgccgctggc acggccaaat cagcctcggc tacggctata acagcaatgt
     5461 aaaccaaggc aacggcatca accaatgcgt gtgggaaatt gcgggcatgt gcctgatgga
     5521 gcgcaccctg cccgcgccaa cggattcgac cttctcatcc tatagcgcga ccgccgaaaa
     5581 aaccgtcccg ctcaaagggc atcacggcgt acaggtgcgc ggtgtactgt acggcaaccg
     5641 ctacacggaa aaagacaaag attcggcggc catgccggat tacggctatc gcaacggttc
     5701 gctgtacgcg ggatacgcct atgccgacgc acgctcctcc ttttccctgc tgccttattt
     5761 cgaatacgac ttccgcaacc gccacaccca ctaccgcgca tggggtgcgg atgccgactg
     5821 gtcgcgcaca ctctccccgc actggcgcat taactcacat gccggagcaa aaaagaccgg
     5881 atacggcgga caaagcaaaa cttattttgc cgatttcaaa caatacgaac tcggcgcggg
     5941 ggcggaattt tccatcacgc cgaaaagcgg cctgcttgta aatttcgatg ccgcacgcaa
     6001 agcctatccc gaaaaatcct cttccagcaa agaatatacg gctcggctgg gcgcgtatag
     6061 gcttttttcg ggcggtactt atttaaacgc cgtcctgctc taccgccgca gcctgtatga
     6121 cgcggcaagt ttcgtcagcg ataacaaacg gcggagcgac aagcaataca tcatgatggc
     6181 tgcggcgggt tttccgcaat ggaatatcaa aggcgtatat cccgaactgc gtttcaggcg
     6241 aacaatcgca cacagcaacg cggtgtatta ccgctaccgc caaaacgaat ggctgttggg
     6301 ttttaaatat cggttttgac cagtcgggtg ataaaaaatg ccgtctgaaa gggcgcttcc
     6361 tttcagacgg catttgtccg taatgtttac agatgaggat tcatcaggtt ctcgggggag
     6421 aggatgcggt tgagttcttc ttcgctcaac aacccgcgtt ccagtacgac ttcgcgcacg
     6481 cctttgccgg tctgggcgca gattttgccg accaagtcgc cgttgcggtg gccgatgtac
     6541 ggattcagat aagtcaccag cccgatggag ttgaaaacgt aacgctcgca gatttcgcgg
     6601 ttgaccgtaa tgcctttgac gcatttgtcg gataggttga ctgcggcatt gcccaagagg
     6661 gaaatggttt caaacataca ttgggcgatg accggctcca taacgtttaa ttgcagttgc
     6721 ccggattcgg cggcgaaggt aatcgtcgta tcgttgccga tgactttgaa gcagacttgg
     6781 ttgacgactt caggaatcac gggattgact ttggcaggca tgatggaaga accggcctgc
     6841 aattcgggca ggttgatttc tttcaaaccg gcgcgcggac cggaagagag aaggcgcaaa
     6901 tcgttgcaga ttttagagag tttgacggcc gtgcgtttca atgcgccgtg taccatcaca
     6961 tatgcaccgc agtcggaggt cgcttcgatc aggttttcag tcagtttgca aggcaagccg
     7021 ctgacttcgg agagtttttt gaccaccagt tcggcgtagc ctttgggcgt gttcacgccc
     7081 gtgccgattg ccgttgcgcc caaattgact tccaagagca gttggcgggt gcggtcaagg
     7141 ttgaggattt cctcttccaa caatacttgg aaagattgga attcctggcc tgccgtcatc
     7201 ggtacggcat cttgaagctg ggtgcgaccc attttcaaaa cgtctttaaa cgcttcggct
     7261 ttggcggcaa aggcgttttt caatacggtc agtttgtcga gcaattcgcc gatgctgtaa
     7321 tacacggcaa ggcggaagcc cgtgggatag gcatcgttgg tggattggct ggcgttgacg
     7381 tgatccatcg gattgacgat gtcgtagcgg cctttctcat agcccaaggc ttccaatgca
     7441 aggttggcga tgacttcgtt ggtgttcata ttgaccgaag ttcccgcccc gccctgatac
     7501 acgtcggacg ggaattggtc gaggcagcgg ttgttcagca gaacttcgtc gcaagccttt
     7561 tcaatggcgg cggcgatttc gggttttact gcacccaatt cgccgtttgc ctgcgcggtt
     7621 gcttttttca ccatcaccat actgcggaca aactgcggca cgtcggaaat tttttgtgtg
     7681 gagattttaa agttttcaat ggcgcgcagg gtgtggatgc cccaatacac ttcggcggga
     7741 atctcgcggt cgcccaataa atcgtgttcg atacggacag tcatgttttt acctttgtaa
     7801 gtcggataat taatattgaa aaaatgcgcc atcggaaaga tgccgcagca ggacgaacac
     7861 tat
//
LOCUS       DAWTHD010000100         7635 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWTHD010000100 DAWTHD010000000
VERSION     DAWTHD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.100"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            129..1091
                     /locus_tag="WM704_001765"
     CDS             129..1091
                     /locus_tag="WM704_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HFA9341131.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            1138..1509
                     /locus_tag="WM704_001766"
     CDS             1138..1509
                     /locus_tag="WM704_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFA9341132.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            1535..2461
                     /gene="fabD"
                     /locus_tag="WM704_001767"
     CDS             1535..2461
                     /gene="fabD"
                     /locus_tag="WM704_001767"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFA9341133.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            2600..4465
                     /gene="msbA"
                     /locus_tag="WM704_001768"
     CDS             2600..4465
                     /gene="msbA"
                     /locus_tag="WM704_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFA9341134.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAGIISTLQNWREQFTYMVWGTENKIWTVPL
                     FLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLMN
                     MLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRYY
                     RDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSKK
                     ITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKSL
                     ANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKAL
                     DNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLRA
                     QFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIGS
                     NGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGII
                     VAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            4565..6130
                     /gene="guaA"
                     /locus_tag="WM704_001769"
     CDS             4565..6130
                     /gene="guaA"
                     /locus_tag="WM704_001769"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFA9341135.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            6192..6938
                     /gene="fabG"
                     /locus_tag="WM704_001770"
     CDS             6192..6938
                     /gene="fabG"
                     /locus_tag="WM704_001770"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFA9341136.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            7003..7488
                     /locus_tag="WM704_001771"
     CDS             7003..7488
                     /locus_tag="WM704_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFA9341137.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPTVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
BASE COUNT         1833 a         2407 c         1888 g         1507 t
ORIGIN      
        1 accatctgta tgccaaacca cacccccatc cctgtttccg tcatttgcac cctgcccgta
       61 ttgggcaatc atcggttttt cgcttacaat agctgaatct gaaccaactc tctaaaaagg
      121 ccgttcccat gcagtatgca aaaatttccg gcacgggcag ctatcttcct gccaaccgcg
      181 tcagcaatga cgaccttgcc caaaaggtag atacctctga cgagtggatt accgcgcgta
      241 cgggcatcaa attccgccat attgcagccg aaaacgaaaa aaccagcgat cttgcagccg
      301 aagcggcgcg gcgcgctttg gcagatgcca aattaaatat aaacgacatt gatttgatta
      361 tcgtggcgac ggcaacgccg gatatgcagt ttccttctac cgccaccatc gtgcagcaaa
      421 aattgggcat caccaacggc tgccccgcgt ttgacgtgca ggcggtgtgc gcaggcttta
      481 tgtacgcgct gactacggca aatgcctaca ttaaaagcgg tatggcgaaa aacgcgctgg
      541 tcatcggcgc ggaaaccttc agccgcatcg tcgattggaa cgaccgcaca acctgcgtgc
      601 tgttcggcga cggcgcgggc gcggtggttt taagcgcgtc cgacaaaccg ggcatcatcc
      661 acagcaaact caaagccgac ggcaattatc tgaaactctt aaacgtcccc gggcaaatcg
      721 cctgcggcaa agtttccggt tcgccgtaca tttcgatgga cggtcccggc gtgttcaagt
      781 ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat cgaagaagca ggttacaccg
      841 ccgaacaaat cgactggatt gttccccatc aggcaaaccg ccgcattatc gaatcgaccg
      901 cgaaacattt gggtttgagt atggacaaag tcgtcctgac cgtccaagac cacggcaaca
      961 cgtccgccgc atccattccg ctggctttgg acgcgggcat ccgcagcgga caaatcaaac
     1021 gcggtcaaaa cctgctgctc gaaggcatag gcggcggttt cgcgtggggc gcggtgctgt
     1081 tgcaatattg aaaccggtgc cgtctgaaac aggctttcag acggcatttc ccatatcatg
     1141 aagcggcagg ctttcttcaa accgatggcg tgtgcggcat ttctgtccgc cgtttcgctg
     1201 cgcctccccg tattgggcgc gtgttacgca atattgtccc tctatgcgtt tgcactttac
     1261 ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca ttcccgaaca ccgcctgctc
     1321 ctgcctgcct tgttcggcgg ttggacgggc gcatacttgg gtagtaggat gttcaggcat
     1381 aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga ctgtttcggg caatgtcctg
     1441 gcgacctgca tcctgattga ttatttcgtt ccgcccgaac tttttgtaaa actcgggcaa
     1501 catctctgac aatccaaccc aacaaaggaa tactatgtct ttcgcattct tcttccccgg
     1561 acaaggttcc caaagcctcg gcatgatgaa cggctttgcc gaacacgcca tcgtcaaaaa
     1621 cacatttgac gaagcctccg ccatattggg gcaggacttg tgggcgatga ttaacggcag
     1681 cgatgccgaa atcatcggtc aaacagtcaa cacccagccc attatgctcg ccgccggcgt
     1741 tgccgtttac cgcgcctatt tggaagtggg cggcaaaacg cctgccgccg ttgccggaca
     1801 cagcctcggc gaatacaccg cgcttgtcgc cgccgaagca ttggattttg ccgatgccgt
     1861 caaactcgtc cgcctgcgcg ccgaactgat gcagtccgcc gtaccgcaag gcgtaggcgc
     1921 aatggcggcc attttgggtt tggaagatga acaggtcagg cagatttgtg ccgaatccgc
     1981 acagggcgaa gtggtcgaag ccgtcaactt caactcgccc ggacaagtcg tgattgcagg
     2041 caacgccgcc gccgtcggac gcaccatggc tgccgccaaa gaagccggag ccaaacgcgc
     2101 cctgccgctg ccggtatccg ttccttccca ttgcagcctg atgaaacccg ccgccgacaa
     2161 acttgccgaa gcactcaaaa ccgttgaaat caagcagccg caaatccgtg tcatccacaa
     2221 cgccgacgtt gccgcctacg atgatgccgg caaaatcaaa gacgcgctcg tccgccagct
     2281 ttacagcccc gtgcgctgga cggaaaccgt caacgccctt gtttcagacg gcattgccga
     2341 atccgccgaa tgcggtccgg gcaaagtgtt ggcgggcttg gcaaaacgca tcaacaaggc
     2401 cgccgcttgc agcgcactga ccgatgccgg acagattacc gcctttatcg aagcgcactg
     2461 acttcgttcc gcaaaaagca gcctgccctc ttcaggctgc ttttcacgtc cgaacggcaa
     2521 acagccgcat atttgcgcta taattcatcc ctaccaaacc accgacagcg gctgccgttg
     2581 cagttcccgc cctaccgata tgatagaaaa actgactttc ggactgttta aaaaagaaga
     2641 cgcgcgcagc tttatgcgcc tgatggcgta cgtccgcccc tacaaaatcc gcatcgttgc
     2701 cgccctgatt gccattttcg gcgttgccgc caccgaaagc tacctcgccg ccttcatcgc
     2761 ccccctgatc aaccacggct tttccgcacc cgccgcgccg cccgagctgt ccgccgccgg
     2821 catcatttcc accctgcaaa actggcgcga acagtttacc tatatggttt gggggacgga
     2881 aaacaaaatc tggaccgtcc cgctcttcct catcatcctc gtcgtcatcc gtggcatctg
     2941 ccgctttacc agcacctatc tgatgacttg ggtctccgtg atgaccatca gcaaaatccg
     3001 caaagatatg tttgccaaaa tgctgaccct ttcctcccgc taccatcagg aaacgccgtc
     3061 cggcaccgta ctgatgaata tgctcaacct gaccgaacag tcggtcagca acgccagcga
     3121 catcttcacc gtcctcacgc gcgacacgat gatcgttacc ggcctgacca tcgtcctgct
     3181 ttacctcaac tggcagctca gcctcatcgt cgtcctgatg ttccccctgc tctccctgct
     3241 ctcgcgctac taccgcgacc gtctgaaaca cgtcatttcc gactcgcaaa aaagcatagg
     3301 cacgatgaac aacgtgattg ccgaaaccca tcagggacac cgcgtcgtca agctgttcaa
     3361 cgggcaggcg caggcggcaa accggttcga cgcggtcaac cgcaccatcg tccgcctcag
     3421 caaaaaaatc acgcaggcaa cggcggcaca ttccccgttc agcgaactga ttgcctcgat
     3481 cgccctcgcc gtcgtcatct tcatcgccct gtggcaaagc caaaacggct acaccaccat
     3541 cggcgaattt atggcattca tcgtcgcgat gctgcaaatg tacgccccca tcaaaagcct
     3601 tgccaacatc agcatcccta tgcagacgat gttcctcgcc gccgacggcg tatgtgcatt
     3661 tctcgacacc ccgcccgaac aggacaaagg cacgctcgcg ccgcagcgtg tcgaagggcg
     3721 catcagcttc cgcaacgtcg atgtcgaata ccgttcagac ggcatcaaag ccctcgacaa
     3781 cttcaacctc gacatcagac aaggcgaacg cgtcgccctg gtcggacgtt ccggcagtgg
     3841 caaatccacc gtcgtcaacc ttctgccccg ctttgtcgaa ccgtctgccg gcaacatctg
     3901 catagacggt atcgacatcg ccgacatcaa actcgactgc ctgcgcgccc aattcgccct
     3961 cgtctcccaa gacgtattcc tgtttgacga caccctgttt gaaaacgtcc gatacagccg
     4021 tcccgacgct ggcgaatccg aagtcctgtc cgccctccaa gccgccaacc tgcaaagcct
     4081 gatcgacgca tccccgctcg gactgcacca gcccatcgga tcgaacggca gcaacttatc
     4141 cggcggacag cggcaacgcg tcgccattgc ccgcgccatt ttgaaagacg cgccgatatt
     4201 attattggac gaagccacca gcgcattgga caacgaatcc gaacgcctcg tccaacaagc
     4261 gctcgaacgc ctgatggaaa accgcaccgg catcatcgtc gcccaccgcc tgaccaccgt
     4321 cgaatccgcc gaccgcatca tcgtcatgga tggcggcaaa atcatcgaac aaggcacaca
     4381 cgatcaactc atgttccaaa acggctacta caccatgctg aggaacatct ccggaaaaga
     4441 taccgccgcc gttcagacgg cataaacaaa atgccgtccg aaatggtata atcgccccga
     4501 ccccttcaga cggcactata tccgccgacc catccgatta tcttcaacca ccgtaaaacc
     4561 cattatgacc caagacaaaa tcctcatcct cgacttcggt tcgcaagtta cccggctgat
     4621 cgcccgccgc gtgcgcgaag cacacgttta ctgcgagctg cattccttcg atatgccttt
     4681 ggacgaaatc aaagccttca accccaaagg catcatcctt tccggcggcc ctaattctgt
     4741 ttacgaatcc gactatcaag ccgataccgg tatttttgat ttgggcattc cggttttggg
     4801 catctgctac ggcatgcagt ttatggcgca ccacttgggt ggcgaagtgc agcccggcaa
     4861 ccagcgcgaa ttcggttacg cgcaagtcaa aaccatcgac agcggactga cacgcggcat
     4921 tcaagacgac gcgcccaaca cactcgacgt atggatgagc cacggcgaca aagtgtccaa
     4981 actgcccgac ggtttcgccg tcatcggcga cactccgtcc tgcccgattg ccatgatgga
     5041 aaacaccgaa aaacaattct atggcatcca gttccacccc gaagttaccc acaccaaaca
     5101 aggccgcgcc ctgttgaacc gctttgtctt ggatatttgc ggcgcgcaac cgggctggac
     5161 gatgcccaac tacatcgaag aagccgttgc caaaatccgc gaacaagtcg gcagcgacga
     5221 agtgatttta ggtctgtccg gcggcgtgga ctcttccgta gccgccgcgc tgattcaccg
     5281 cgccatcggc gaccaactga cctgcgtgtt cgtcgatcac ggtttgttgc gcctgaacga
     5341 aggcaaaatg gtgatggata tgttcgcccg caacttgggt gtgaaagtga tacacgtcga
     5401 tgccgaaggg cagtttatgg cgaaactcgc cggcgtgacc gaccctgaga aaaaacgcaa
     5461 aatcatcggc gcggaattta tcgaagtatt tgatgccgaa gagaaaaaac tcaccaacgc
     5521 caaatggctg gcgcaaggca cgatttaccc cgacgtaatc gaatccgccg gtgcgaaaac
     5581 caaaaaagcc cacgccatca aatcccacca caacgtcggc ggcctgcctg aaaatatgaa
     5641 gctcaaactg cttgagccct tgcgcgactt gttcaaagac gaagtgcgcg agttgggcgt
     5701 ggctttgggc ctgccgcgcg aaatggtgta ccgccacccc ttcccgggcc ccggtttggg
     5761 tgtgcgcatc ttgggcgaag tgaaaaaaga atacgccgac ttgctgcgtc aggcggacga
     5821 tattttcatc caagaattac gcaatactac cgacgaaaac ggcacgtctt ggtatgacct
     5881 gaccagccag gcatttgccg tattcctgcc cgtcaaatcc gtcggcgtga tgggcgacgg
     5941 ccgcacttac gactacgtcg tcgcactgcg cgcagtcatc accagcgact ttatgactgc
     6001 acactgggca gagctgccat actcactgct cggccgcgtg tccaaccgca tcatcaacga
     6061 agtcaaaggc atcaaccgcg tggtgtacga tgtcagcggc aaaccgcccg ccaccatcga
     6121 gtgggaataa acagcaaaca gggctgcacc gtccggcgca gtccttcgat tatcggaaaa
     6181 aaggaaaaaa tatgagcaca caagatttaa gcggcaaaat cgctttggta accggcgcat
     6241 cgcgcggcat cggcgcggca attgccgaca cactggcggc agccggtgcc aaaatcatcg
     6301 gtacggcaac cggcgagagc ggtgcggcgg cgattagcaa acgtctggca caatggggcg
     6361 gcgaaggccg cgtattaaat tccgccgaac ccgaaaccgt cgaaaacctg attgccgaca
     6421 tcgaaaaaac gttcggcaaa ctcgacattc tggtcaacaa cgccggcatc acccgcgaca
     6481 acctgttgat gcgtatgaaa gaagaagagt gggacgacat catgcaggtc aacctcaaat
     6541 ccgtgttccg cgcttctaaa gccgtcttgc gcggcatgat gaagcagcgc gccggccgca
     6601 tcatcaacat cacctccgtc gtcggcgtga tgggcaatgc cgggcaaacc aattatgccg
     6661 ccgcaaaagc gggcttgatc ggttttgcca aatctatggc gcgcgaagtc ggcagccggg
     6721 gcattaccgt caactgcgtc gccccgggct ttatcgatac cgatatgacc cgcgccctgc
     6781 ccgaagaaac ccgccaaaca tttaccgccc aaaccgcctt gggcagattc ggcgacgcgc
     6841 aagacatcgc cgatgcggtt ctgttcctcg cttccgatca ggcgaaatac atcaccggtc
     6901 aaacgctgca cgtcaacggc ggtatgctga tgccctaacg gacaggcttt tcaaccatgc
     6961 cgtctgaagc cctttcagac ggcatttgca ttcccaaaca aaatgaacac tcaccaccac
     7021 ctgccctgcc cctgcggttc aggcacaagc tatgccggct gctgccgacc gcttcatctg
     7081 cgccaaatcc tgccgcccac cgccgaagcc ctcatgcgtt cgcggtacgg cgcgtatgtg
     7141 ctgcacctta tcgactacat catcgctact accgttccgg cgcaacaaac attcctcgat
     7201 gccgccgaac tgatgcagtg gagcagagaa gccgaatggc tgggcttgaa tgtcatcgca
     7261 caccgcaacc tcggcaaaca gcacgcccaa gtcgaatttg aagcctactt ccaagacggc
     7321 ggaaaccgaa cagtgcatca cgagctgtcg gcattcgtga aaatagcgga gcaatggtat
     7381 ttcatcgatc cgactgttcc gctgcctacc atgaaacagc cctgcatttg cggttcgggc
     7441 aaaaaattca aagcctgctg cggcaaatat ctgcaacctg tcgcataaaa atgccgtctg
     7501 aacgttcaga cggcattttc aacgcgcaaa aaaaaaccat tcataccaag ggtaagtatg
     7561 aatggtcaat acattgcggg aaaacatctt acttgctgca ctgccgaaaa gggagaaacg
     7621 gcagtgttaa ccagc
//
LOCUS       DAWTHD010000101         7208 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWTHD010000101 DAWTHD010000000
VERSION     DAWTHD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.101"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            1..92
                     /locus_tag="WM704_001772"
                     /pseudo
     CDS             1..92
                     /locus_tag="WM704_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1069..1752)
                     /locus_tag="WM704_001773"
     CDS             complement(1069..1752)
                     /locus_tag="WM704_001773"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFA9341138.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1901..4204
                     /gene="parC"
                     /locus_tag="WM704_001774"
     CDS             1901..4204
                     /gene="parC"
                     /locus_tag="WM704_001774"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFA9341139.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDRSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAVFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4224..5948
                     /locus_tag="WM704_001775"
     CDS             4224..5948
                     /locus_tag="WM704_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFA9341140.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHYIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            6048..6800
                     /locus_tag="WM704_001776"
     CDS             6048..6800
                     /locus_tag="WM704_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9341141.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1733 a         2131 c         1778 g         1566 t
ORIGIN      
        1 tcccgccgtt cttgagggaa tgagaatttc gacttaactt cggcacaccg tcccggcagc
       61 taaaaatcct gcgggatcgg tgtggaattt agggataatc tagtacagcc cctactattt
      121 tagtagtttt accattagaa aaaggatatg aatggaattg ccttgaggta gaagtactat
      181 ccgctgctca atctctagca aatatctatt gcaacaaggt tcgcctcata gatggtcatg
      241 tttgggagga ataaatgaaa aagcgtagtt ggtaattttt agttgcgcag ggtgggcttc
      301 agcccaccgt tttttcttca ttttgggttg ttgattggtg ggctaaagcc caccctacga
      361 ttactaaaat cccgtcattc ccgcgtagag cgcatcatat aaggcgggaa tccagacctt
      421 tcggtgcgaa aacttatcgg ataaaacggt ttcccctgca tcctggattc ccactttcgt
      481 gggaatgacg ggatataggt ttccctacgg acgtgttcag attcctgcct tcgcgggaat
      541 aacggcggag cagtttctgc ttttccaata aatgaccgta acttaaaatc ccgtcatccc
      601 cgcgaaagca aaaatcctgc ctgtcggatt tcggtttttt cgggaaactg atgaatcgtc
      661 attcccgcgc aggcgggaat ccagaacgcg ggacggcggc aatattcaaa ggttgtctga
      721 aaattcagag gttccagatt cccgccttcg cgggaatgac agcggagcag tttctgcttt
      781 tccgataaat gaccgcaacc taaaacccat ccttcacacg aaaacagaaa aacaaaaacc
      841 taaaatcccg tcattcccgc gataacagtt gcgtaattgc gtagggtggg cttcagccca
      901 ccgtttcttc atttttgctt gtcaattagt ggactgaagc ccaccctgcg gttactgttt
      961 cttctttttc gattattaat tggcaggctg aagccctccc tacgtttacc gtcaaaaatg
     1021 ccgtctgaaa tgctcaagcc ctcagacggc attttttatt tcgcaccctt acaggtaaaa
     1081 tttctcgatg actttcaggt tgtcgtctaa tttgtacacc agcggctggc cggtcgggat
     1141 ttccaagccc atgatgtctt cgtcggaaat gccctcgatg tgttttgcca gcgcgcgcag
     1201 ggagttgccg tgcgccgcta ccaagacgcg tttgccgctc aaaatcgcgg gggcgatttg
     1261 gtcttcccaa aacggtaata cgcgctccag cgttactttc aggttttcgc cgtcgggtac
     1321 gacatcggca ggcagatggg catagcgacg gtctttgtgt gcggaaaacg cgtcgtcttt
     1381 gtccaaaagc ggcggcaggg tgtcgtagct gcgccgccag atgcggactt gctcgtcgcc
     1441 gtatttttcg gcggtttgtt ttttgtccaa accttgcagt cggccgtagt ggcgttcgtt
     1501 cagcctccat gttttgattt gcggtacgaa cagttggtcg gattcttcca aaacgatgtt
     1561 gcaggtctta atcgcgcggg tcaggacgga tgtgaaggcg atgtcgaact catagccgtt
     1621 ttctttcagt tttttaccgg cggcggcggc ctcggcaagc ccctgctcgc tcagcttcac
     1681 gtcgcgccag cctgtaaaca ggtttttcgc gttccattcg ctttgtccgt ggcggataaa
     1741 taccaattcc atatcgtctc caatgtgtga aagtgggaaa gctttattta taacatattt
     1801 tcacatttcc cgtatttgat tcagattcag gcacggcgcc cactatggtt tgccattttg
     1861 atttacaata atgtcctttg ctttacattc cgcatacaca atgaatacgc aaccgcacgc
     1921 ttcccatacc gattccaaca cgctgatgct cggccgatac gccgaacgcg cctatctcga
     1981 atacgccatg agcgtggtca aaggccgcgc gctgcctgaa gtttcagacg gccaaaagcc
     2041 cgtgcagcgg cgcattttgt ttgccatgcg cgatatgggt ttgacggcgg gggcgaagcc
     2101 ggtgaaatcg gcgcgcgtgg tcggcgagat tttgggtaaa taccatccgc acggcgaccg
     2161 ttccgcctat gaggcgatgg tgcgcatggc tcaggatttt accttgcgct accccttaat
     2221 cgacggcatc ggcaacttcg gttcgcgcga cggcgacggg gcggcggcga tgcgttacac
     2281 cgaagcgcgg ctgacgccga ttgcggaatt gctgttgtcc gaaatcaatc aggggacggt
     2341 ggattttatg ccgaactacg acggcgcgtt tgacgagccg ctgcaccttc ccgcccgctt
     2401 gcctatggtg ttgctcaacg gcgcgtcggg catcgcggtg ggtatggcga ccgagattcc
     2461 gtcgcacaat ttgaacgaag tcacgcaggc ggcgattgca ctgttgaaga aaccgacgct
     2521 ggaaaccgcc gacctgatgc aatatattcc tgctcccgat tttgccggcg gcggtcaaat
     2581 catcacgccg gcggacgaat tgcgccgtat ttacgaaacc ggcaagggca gcgtgcgcgt
     2641 gcgtgcgcgt tatgaaatcg agaaattggc gcgcggacag tggcgcgtca tcgtaaccga
     2701 actgccgccg aacgccaatt ccgccaaaat ccttgccgaa atcgaagagc aaaccaaccc
     2761 gaaaccgaaa gcgggcaaga agcagctcaa ccaagaccgg ctcaatacca aaaagctgat
     2821 gctggattta atcgaccgcg tgcgcgacga gtccgacggc gaacatcccg tgcgccttgt
     2881 atttgaaccc aaatccagcc gcatcgatac cgataccttc atcaacacgc tgatggcgca
     2941 aacttcgctg gaaggcaatg tgtccatgaa cttggtgatg atgggtttgg acaaccgccc
     3001 cgcgcagaaa aacctgaaaa cgattttgca ggaatggctg gatttccgcg tcgttaccgt
     3061 aacacgccgt ctgaaattcc gtttaaacca agtggaaaaa cggctgcaca tcctcgaagg
     3121 ccgtctgaaa gtctttctgc acatcgacga agtgattaaa gtcatccgcg aatcggacga
     3181 cccgaaagcc gatttgatgg cggtgttcgg gctgaccgaa atccaagccg aagacatttt
     3241 ggaaatccgc ctgcgtcagc tggcgcgttt ggaaggtttc aaactcgaaa aagaattgaa
     3301 cgaattgcgc gaagaacaag gccgtctgaa catccttttg ggcgacgaaa acgaaaaacg
     3361 caagctgatt atcaaagaga tgcaggcgga catgaagcag ttcggcgacg cgcgccgcac
     3421 gctggtggaa gaggccggac gcgccgtgct gacacaaacc gccgccgacg aacccatcac
     3481 gctgattttg tcggaaaaag gctggatacg cagccgtgcc ggacataatc tcgatttgag
     3541 ccaaaccgcg ttcaaagaag gcgaccgcct caaacaaacc cttgaaggcc gcactgtttt
     3601 acccgtcgtc atcctcgatt catcgggcag aacctactcg atcgatgccg ccgaaatccc
     3661 cggcggacgc ggcgacggcg taccggtttc ctccttaatc gagttgcaaa acggcgcgaa
     3721 acccgtcgcg atgttgacag gattgccgga acaacattat ttattatcaa gcagcggcgg
     3781 ctacggcttt atcgccaagc tgggcgatat ggtcggacgc gtgaaagcgg gcaaagtggt
     3841 gatgaccgca gacagcggcg aaaccgtcct gccgccggtt gccgtctatg cctcctcgtt
     3901 catcaacccc gactgcaaaa tcattgcagc caccagtcaa aaccgcgccc tcgccttccc
     3961 catcggcgaa ttgaaaatta tggcgaaagg caaaggactg caaatcatcg gattaaacgc
     4021 cggcgaatcg atgacgcata ccgccgtttc ttccgagccg gaaatcctga ttgaaagcga
     4081 aggcaggcgc ggcgcggcgc acaaagaccg cctccccgtc gccctgattg aggcaaaacg
     4141 cggcaaaaaa ggcagactgt tgcccatatc gggcagcctg aaacagcttt cttcccccaa
     4201 ataaacccgg ttccgcacat attatggtga tttccaaccc ccgcgaactt gaaaaactca
     4261 aagaccggat tcccaatctg atcaacatca tccgcgtcgc catcgttttc ccgctgatga
     4321 ttatgcacat cctcgggctg gaaaccggca gccgtgcgaa cctgcacgcc tcatggacgg
     4381 cgtgggcgtt ttacttgtgg cttgccattg cctgctggct gattttcttt tccaccctca
     4441 acccgcaatg gcaatggcaa tggcaggctt tgagaatacc gagtttcagc gccgtggccg
     4501 acatcacgat gatcggcgtg ctgacctacc tgttcggcgg catcgattcg gggttcggca
     4561 tcctgatcct gcccttcgtg ggcagctcct gcctactcag ttacgggcgt tatcccctgc
     4621 tctatgccag ctatgcatcc atcctgctga tattcaacgc ccttgcagac agcaatatca
     4681 atatgtatcc gctgattctg gatgcaaaaa ccgtcaccaa taccttcgtc gtcgtagccg
     4741 gctcctattt cgtcgctatg attgcctcgc tgtcggtcag atacatcgac cgtgccggca
     4801 aacttgccca tgaaaaccac gtcgcctacc gccgtatcag gggcttgaac caaatcgtgc
     4861 tcaaccgcgt tcaggaagcg gtcgtcgtca tcaacgtcga gcatcagacc atactgttca
     4921 ataaaaaggc aaaagatctg ctccccatgc ttgaaatcgg acagcatacc tccctgttcg
     4981 accctgtcgc catcttatgg gataaaacat cctcacgcac cttcgaacac tatatcgaca
     5041 cgcccgaact gaccgcccgc atccgcgccg tgccgatgaa caaaaagcag aacaagctgc
     5101 tcatcctcta tatccgcccg caaagcgaaa ttcaggcgga agccctgtcc gtcaaacttg
     5161 ccgcgctcgg acaactgacc gccaaccttg cccacgaaat ccgcaacccg atgtccgcca
     5221 tccgccacgc cgacggcggc actttatttt tggacgaagt ggccgacctg cccctttcca
     5281 tgcaggtgaa actcttgcgc gcgattcaag aaaaagccgt gcgccgtatc ggcgacgcga
     5341 ccgagcagcc cgtcgatgtc cgcatcgtct gcgccaccca caaaaacctc gaagcccttg
     5401 tcgaaagcgg cgcattccgc caagacctgt attaccgtct caatgtcgtc agcctcaata
     5461 tgccgtccct gcgtgaaatg cgcgaaaatt tgaagctgcc caccccctac ctcctgtaca
     5521 aacacagcca caacaaccgg ccctacacac tctcccccgc cgcgcaacag atgctcctga
     5581 attacagtta tccgggcaat ttccgcgaac tcgaaaacat cctcgaacgc gccgtcgccc
     5641 tgtgcgtcgg atatacagtg caaatcgacg acctgcaaat ccaagatgtg caccacaaac
     5701 ccgtccggac ggaaaccgcc gtccccgttg ccgatacctt gccgtctgaa acagccgccg
     5761 caccgtcccg ccttctcccg ttcgaccccg ataccatgca gatacaggac tatctcgaca
     5821 aaatcgaacg cgacatcatc ggacaagtcc tcaaacaaac cgaaggcaac cgcacgcaag
     5881 ccgccaaacg cttgggcatc agcttccgct ctatgcgcta ccgtatggaa cgcctcaaca
     5941 tagactgaca aacggcatcc gcaccatctc cgcccacccg aaaaaatgcc gtctgaaacg
     6001 gcacggggaa gcgggttcgc cccacgaccg aacggacaca atacaccatg accgacatcc
     6061 tcattgacga taccgccacc gaagccgtcc gcaccctgat acgggcattc cccctcgtgc
     6121 ccgtttccca accgcccgaa caaggcagtt acctccttgc tgaacacgat accgtcagcc
     6181 tcaggcttgt cggggaaaaa agcaacgtca tcgtcgattt tacctccggc gcggcacaat
     6241 accggcgcac aaaaggcgga ggagaactca tcgccaaagc cgtcaaccac accgcgcacc
     6301 ccaccgtatg ggatgcaacc gcaggattgg ggcgcgacag cttcgtcctc gcctcgctcg
     6361 gactgaccgt taccgccttc gagcaacatc ccgccgtcgc ctgcctgctt tcagacggca
     6421 tccgccgcgc cctcctcaat cccgaaacgc aagacaccgc cgcgcgcatc aacctccatt
     6481 tcggcaatgc cgccgaacaa atgcctgccc ttgttaaaac acaaggcaaa cccgacatcg
     6541 tttatctcga cccgatgtat cccgaacgcc gcaaaagtgc cgccgtcaaa aaagaaatgg
     6601 cttatttcca ccggcttgtc ggcgaagcgc aagatgaggt catcctcctt cataccgcac
     6661 gccaaacggc aaaaaaacgc gtcgtcgtca aacgcccgcg cctcggcgaa caccttgccg
     6721 gacaagcccc cgcctaccaa tacaccggca aaagcacccg cttcgacgtt tacctgccct
     6781 acggagcgga caagggataa cgcccataaa acaagacacc gaaaaatttg ccgttcttat
     6841 gcaaacgaga aaccggtttt tgcgtttcga ctgttttgga taagtcatca caccttaaag
     6901 tttgtcattc ccacggaagt gggaatccga ttcgttcagt tttagttgtt ttcaagtttc
     6961 aggcaacttc caaaccgtca ttcccgcgaa agtgggaatc cggaaatgaa aggcaacagg
     7021 aatttatcgt aaatgactga aaccgaacgg actagattcc cgcctgcgcg ggaatgacgg
     7081 ggcgggagga tgccgtctga aattccgtca ttcccgtgaa aacgggaatc tagaacttct
     7141 gatttttcag acgacttttg aagattgccg ctacccaatg atctggattc ccgcttttgc
     7201 gggaatga
//
LOCUS       DAWTHD010000102         7100 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWTHD010000102 DAWTHD010000000
VERSION     DAWTHD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.102"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(224..688)
                     /locus_tag="WM704_001777"
     CDS             complement(224..688)
                     /locus_tag="WM704_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFA9341142.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            910..1968
                     /gene="alr"
                     /locus_tag="WM704_001778"
     CDS             910..1968
                     /gene="alr"
                     /locus_tag="WM704_001778"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFA9341143.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(2510..3865)
                     /locus_tag="WM704_001779"
     CDS             complement(2510..3865)
                     /locus_tag="WM704_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFA9341144.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(3880..4152)
                     /locus_tag="WM704_001780"
     CDS             complement(3880..4152)
                     /locus_tag="WM704_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFA9341145.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(4277..5014)
                     /locus_tag="WM704_001781"
     CDS             complement(4277..5014)
                     /locus_tag="WM704_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFA9341146.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(5015..5140)
                     /locus_tag="WM704_001782"
                     /pseudo
     CDS             complement(5015..5140)
                     /locus_tag="WM704_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
     gene            complement(5152..6051)
                     /gene="prmB"
                     /locus_tag="WM704_001783"
     CDS             complement(5152..6051)
                     /gene="prmB"
                     /locus_tag="WM704_001783"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFA9341147.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            complement(6150..6347)
                     /locus_tag="WM704_001784"
     CDS             complement(6150..6347)
                     /locus_tag="WM704_001784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341148.1"
                     /translation="MGERLVQTDYAHPAVGIADIGHLVVFCGYPLLALNKPRFFPAYQ
                     VAHTVRHRRFRSGNGLRAAFM"
     gene            6257..6868
                     /locus_tag="WM704_001785"
     CDS             6257..6868
                     /locus_tag="WM704_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341149.1"
                     /translation="MDNRKRRQGVRCLQCQRRDGRNRFARAFLPRCVDDTRASKWQVR
                     GCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELGEFAETKELRKAAMQYARDNFV
                     GKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVALDKVLMNAKYEGSFPNKLGRD
                     HIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDHFILKDK"
     gene            7008..>7100
                     /locus_tag="WM704_001786"
     CDS             7008..>7100
                     /locus_tag="WM704_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFA9341150.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAAD"
BASE COUNT         1919 a         1752 c         1623 g         1806 t
ORIGIN      
        1 aatttaacaa gaaaaacgct tgctttttga ccggaaaagc tgcataattc gttccgttag
       61 ctggttcgag tagtcagtta atagtttctc ctctatttct cctttgtaga cttggcacac
      121 attctattga atgtgtgcat tttttatctg aagcaacaag cctctgtgcg tgatgttgtt
      181 atgtttcatt tagatgtcaa accgcatacc cggtctgaaa tattcaatcc aaatccaaaa
      241 ccggattttc tttgacttcc tccatcacaa cataactcct actctccgaa gcggcaggca
      301 gttgcaatag gatattgccg agcatatccc gataggcaga catatcgggc aaacgtactt
      361 taatcaggta gtcgtattcg cccgacacca gatggcactc cataatttgc ggaattttca
      421 gcacttcttt tttgaaatct tcgaaaatat tgcccgattt ggattgcagc ttcagctcga
      481 caaaaaccaa tggcggtttt cccaacagat ggggattgag atgggcgtga tagccggaaa
      541 tataatgttc ccgctccaaa cggcataccc tctctgtaac gggcgtgctg gacaacccta
      601 ccttctcggc aagctccgtc atcgggatac gggcattttg ctgaagaatt ttgaggatgc
      661 ggaaatcgat tttatctagt tctttcattt ggattacctt gtatttatta ttgattttaa
      721 caaatagagt ataaatttgg gaaagcaatt atatcaggaa aagcaaaccg ccttcctacc
      781 tgaaaactgc tgctctggct tgaagacaca aggttcttta atattttaaa agccttgccg
      841 ttggattata attccccaat tgatttctta attttgctaa taaacacttg tttggtaagg
      901 aatgaattta tgcggccttt gaacgtgcag atcaggttgg gcaaccttag gcacaattat
      961 cggattttga aggaaatgca cggaggcaag ctgttggcgg tagtgaaggc cgacgcatac
     1021 ggacacggtg cggtcagatg tgctttcgcg ctggcagact tggcagacgg ctttgccgtg
     1081 gcgacaatcg acgagggaat caggctgcgg gagagcggca ttacccatcc gattgtcctt
     1141 ttagaaggcg tatttgaagc atcagagtac gaagcggtcg aacaatactc gctttggccg
     1201 gcagtcggaa accaatggca gcttgaggct ttgctttccc gccattggaa aaaacctgtc
     1261 aaggtctggt tgaaaatgga ttcgggaatg caccgtaccg gatttttccc tcatgattac
     1321 gcttcggcat atgcggcatt gaaacagtcg gaatatgtgg acagtattgt caagttctcg
     1381 catttctcct gtgcggacga acctgaaagc ggtatgacgg aaatacagat ggaagcattc
     1441 gatttgggta cgaaagggct ggaaggcgaa gaaagccttg ccaattcggc agctattttg
     1501 aatatccccg aagcacgcag ggattggggg cgcgcgggct tggcgttata cggcatttcc
     1561 ccgttcggag gcagcgatga caggctgaaa cccgtgatga ggctttcaac ccgtattttc
     1621 ggcgaacgcg ttttacagcc gcactcccct atcggttatg gcgcaacatt ttataccagt
     1681 aaatctacgc gcgtcggtct gattgcctgc ggttatgcgg acggttatcc gcgccgcgct
     1741 ccaagcaatt cccccgtcgc cgtcgacggt aaattgaccc gggtcatcgg caggatctct
     1801 atggatatga tgactatcga gctggacgct tcgcaagaag gtttgggaca cgaggtcgaa
     1861 ctgtggggcg atacggtcaa tatcaatacc gttgccgaag cggccggaac catcccttac
     1921 gaattgatgt gcaatatcaa acgtgcaaaa ttcacttata tcgagtaatc gagtccaaac
     1981 gaaaatcccg tctgaagctt ttcagacggc ataaatgcgt tttgacaaaa tcagtaaaag
     2041 acattgccaa atagaaaata ctgataatgc ccacaatctg aaaccccatc cttcccgcaa
     2101 aaacggaaaa tcaaaatcag aaacctaaaa tttcgtcatt cccgcgcagg cgggaatccg
     2161 gttttttgag tttccgtcat ttctgataaa ttctcgtcgt ttttcatttc tagattcccg
     2221 catgcgcggg aatgacaaaa ttaaagtttc agaatttatt tgaacaacaa actagattcc
     2281 cgctttcgtg gggatgacgg cggataggtt ttgttgtttc ggataaattc ctgttgtttt
     2341 ttggtttcca gattcccacc tttaaaagaa tgacgtaaga aagcagaaat aagaacacgg
     2401 ggatttttta aatttgcaat attcttttct aaagacattt tacccaatgc ccataaacaa
     2461 aaatgccgcc tgaagccttt cagacggcat tttcccatca aaaccgcaat cagtttttca
     2521 tcgattgaac cggtgcggga atcctgccgc cccggttgac gaacacttcg cacgaacctt
     2581 cttttgccgg cattacaggc gcgtagccca acagaccgcc gaactcgacg ctgtcgccga
     2641 cggttttgcc cgttaccgga ataatgcgca cggcggtggt tttgctgttg atcatgccga
     2701 tggcggcttc gtcggcgatg atgccggaaa tggtgtgcgc gggcgtgtcg ccgggaacgg
     2761 caatcatgtc caaaccaacg gagcagacgg cggtcatggc ttcgagtttg tccagcgtca
     2821 acacgcctgc ctcggcggcg gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc
     2881 cgctcaaacc gccgaccgcg ctggaagcca tcatgccgcc ctttttcacg gcatcgttca
     2941 gcaatgccaa agctgctgtt gtgccgtgcg taccgcagac gctcaagccc atttcttcaa
     3001 gaatgcgcgc caccgagtcg ccgacggcgg gggtcggtgc cagcgacaaa tcgagaatgc
     3061 cgaacgggat attcagcatt tttgaggctt cgcggccgat gagttcgccc acgcgggtga
     3121 ttttgaaagc ggttttcttc acgacttcgg cgacctcggt caggctgacc gcgtccgaat
     3181 tttccagcgc ggctttgacc acgcctggac cggatacgcc gacattaatc acagcatccg
     3241 cttcgcccga gccgtggaac gcacccgcca taaacggatt gtcttccacc gcgttgcaga
     3301 acacgacgat tttggcgcag ccgaaacctt cgggcgtgat ttcagccgtg cgtttgatgg
     3361 tttcgcctgc cagcttgacc gcatccatat tgataccggc acgcgtgctg ccgatattga
     3421 tggagctgca cacgatatcg gtagttttca tcgcttcggg aacggaacgg atcaacacct
     3481 catccgaagg cgacatacct ttttgcacca gcgcggaaaa gccgccgata aaggacacgc
     3541 cgatggcttt ggctgccttg tccaaagtct gcgccacgct gacataactg tcggctttgg
     3601 tcgccgccgc gatttgggca atcggcgtaa cggaaatgcg ctgattcaca atcggcacgc
     3661 cgtatttggc ggaaaggtgt ttcgccgttg ccaccaagtc tttgccgacc gtggtgattt
     3721 tgttgtaaat gttttggttt aacacgtcga tgtcggtgct gatgcagtcg tgcaaatcaa
     3781 tgccgatggt aatggtgcgg acatcaaaat tctggtcggc aaccattttg acggtttcta
     3841 aaatttcgcc ggattggata ctcatcacat tcctccgact caaatgcggt gcatcgcttg
     3901 gaagatttct tcgttttgca tacggatgtc aagcgcgagt tttttgctct cttccgcaaa
     3961 caaatccaaa atctcctgcc gcgatttagg gcatttcgag gtatccacca agataatcat
     4021 agtaaaaaaa tcgtccatca gctgttggct gatattgagg atgttgattc ggttttccgc
     4081 caaaattttg gaaacatcgt acacgatgcc gacgcggtct ttaccgatga cggtgatgac
     4141 tgaattgttc acaggcttac tccttgcaga tatccgttaa aatccgaaat tataccaccg
     4201 tcggattttg aagaatattg tcaacaatat atacatacaa aatgccgtct gaaactattt
     4261 cagacagcat caagattcag ggttcgatta aataaccatc cttatcccat tgggttttcc
     4321 tgaccaactt gtcatcctga taaacagcct cgctcttttt agaaccatct tcataccact
     4381 ccaaaaccac cccgttgcgt ttatggtggc ggatagacag ttccgaaagt aatcggccgc
     4441 tttcatccca agtcagaatt ttggcaggct catcgttgac cataaccatt tccgtcttga
     4501 tacttccatc ggcataccat tgcttccata cgccgttcgc cttattttgc ttaaactgga
     4561 tttcgctttc cttgccgccg ttacggtaat aacggtatcc cgtaccctca ctcaagccat
     4621 ttttataagg cataaccgca gattttttac cgttcggata ccagttgacc cattccccgt
     4681 ccggcttacc cttgctgaag ccccccgcca tttttttctg accattaaaa tgccacaaaa
     4741 tcaacatacc gttttgcagg gtaggcacaa aagatttgat ttgcgttgaa gcaacgatat
     4801 aaggttcgga atatttcttc atcgacggat aataaaaatc ctgcgcgtgt gcgatacccg
     4861 ccgccacact atattgcctg atataagcgg cagaagacat cgtcgccgtc agtttcccgt
     4921 tctgattaaa ataaacagaa taggtctgcg ccggcaaagc ggccgaaaaa cccaacagta
     4981 cgattgaaaa tacaatccga gataatttct tcattatgat agcgatataa aaaacaatta
     5041 acttataaac aatatggtaa ggatttctca accaagcgtc aaacccgaaa caatgtatcg
     5101 taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccctccaa ctcactcttc
     5161 gcccaataac tgctcgcgcg tcaagaggaa aacaaaaccg tcgcccccgc tggtttccaa
     5221 ccaagtaaaa ggcaactccg gatacgctgc ttccaataca tccctgttat gcccgatttc
     5281 caccagcaat acacctttag gattcagaaa ctttgccgca ttcagaagaa tctgcctggt
     5341 ggcatccaac ccgtccgccc cgctgcccaa cgccaattcc ggttcgtgca aatattcctc
     5401 aggcaaagcc ccaaccgact ctgcatccac ataaggcgga ttggaaacaa tcaaatcata
     5461 agtgccttcc agtccctcaa acaaatcggt atgaataagc tgaatacgct cttccagacc
     5521 ataatcttca atattgatcc ctgcaacttc caaagcatcc aaactcacat caaccgcatc
     5581 aatttgggca tcaggataat ggtgcgccat ctgaatggca aggcaaccgc ttccggtgca
     5641 aagatccaaa gcattatgca ccagctcatc gtattctatc caaggtcgaa gtccgtcacc
     5701 cagcaattca taaataaaag agcgcggtac aatcacgcgt tcatctacat aaaaatcaaa
     5761 ctccccctgc caggcctggt gtgtcaaata agcggcagga atgtgttcga cagcacgacg
     5821 ctcaataacc gccagcactt cctctttttc agcttccaag agttttgcat caagatatgg
     5881 ggcaagcata tccaaaggca aattcaaagt atgcagaatc aaataaaccg cttcatcatg
     5941 cgcattatct gttccatgac caaaaaagag ccctgcctca ttaaaacggc tgactgcaaa
     6001 acgtaaaaca tcacggatag tcgtcaattc ttgtgcggcc tgattaaaca taatatgaac
     6061 cattctgcgt atagatgctt ttaattataa cagaaacaac aagcaaacct tttcatatcg
     6121 ccaaataacc actcaatcta cccatacaac tacataaatg ccgcgcgaag accattgccc
     6181 gaacggaaac ggcgatggcg gacggtatgg gcaacctgat aggctgggaa gaaacggggc
     6241 ttgttgaggg caagcagtgg ataaccgcaa aagacgacaa ggtgtccgat gtctgcaatg
     6301 ccaacggcgg gatgggcgta atcggtttgc acgagccttt ctcccacggt gcgttgacga
     6361 tacccgagca tccaaatggc aggtgcgcgg ttgttcccgt tgtcgggcgg acaggcaagc
     6421 tggcgaaagg cagccgcgcg atggaaacgg gcgatggacg gcaggtgagg ttgtttccgt
     6481 atcgggcggc gaattggggg aatttgctga aacaaaggag cttcgtaaag cggctatgca
     6541 gtatgcgcgt gataactttg tcggcaaaag ctatgtcaat gaaagcagcg ggcatgaact
     6601 gaaggtaact tggcaaggtg tgaaacacgc tgcgtcaaaa tcgaacagca tcgaactttc
     6661 ccttatggtc gcgcttgata aagttttgat gaatgcaaaa tatgaaggaa gttttcctaa
     6721 caaattaggg agggatcata ttattgcggt acacaaatac tcaaccggtg tcagatttgg
     6781 caataaagtc ttaaaaatca ggataatagc ccgtgagaaa tttgatggga taaaacatta
     6841 cgatcatttt attttgaaag acaaatgaaa aaaccctgtc cagtatatct gggatagctt
     6901 atggaagcat ttaacccagc ctcggataag gctttaaatc catcatgcca tttatacggt
     6961 aaaaacaagg gtctgtacta gattagcaga tatgttaccc tcgaaatatg aagataacac
     7021 actgcaaatt aaagaaagaa gtacaaaaag aaccgctccg ttcttttgta ccggaagtta
     7081 ccgcccgttc tgccgccgat
//
LOCUS       DAWTHD010000103         7084 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWTHD010000103 DAWTHD010000000
VERSION     DAWTHD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.103"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            122..298
                     /locus_tag="WM704_001787"
     CDS             122..298
                     /locus_tag="WM704_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341151.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            797..1639
                     /locus_tag="WM704_001788"
     CDS             797..1639
                     /locus_tag="WM704_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341152.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPIDGNSLADIAAMVYYGTRMIESGKDV
                     SAPLKQLGNRQAVTMLTVWHETRSILKRSAAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(1840..2220)
                     /locus_tag="WM704_001789"
     CDS             complement(1840..2220)
                     /locus_tag="WM704_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFA9341153.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(2553..2726)
                     /locus_tag="WM704_001790"
     CDS             complement(2553..2726)
                     /locus_tag="WM704_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341154.1"
                     /translation="MTTAVKPPADLVRPCPKLPHLEGNTGADVLPWSLQVIGLYKDCK
                     ARHGALVRALGAD"
     gene            complement(2710..3054)
                     /locus_tag="WM704_001791"
     CDS             complement(2710..3054)
                     /locus_tag="WM704_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341155.1"
                     /translation="MIGALLKNWKPLLILSAIAFFAVSWQLDRAAQYRRGYGAAVSEV
                     SERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADG
                     VRELNAAVDDGG"
     gene            complement(3055..3594)
                     /locus_tag="WM704_001792"
     CDS             complement(3055..3594)
                     /locus_tag="WM704_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFA9341156.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVGYCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLSDVAATAKQGAGEA"
     gene            complement(3897..4841)
                     /locus_tag="WM704_001793"
     CDS             complement(3897..4841)
                     /locus_tag="WM704_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFA9341157.1"
                     /translation="MNIIGPDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKINDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKPAYYIVKTGQPYDAERH"
     gene            complement(5056..6243)
                     /locus_tag="WM704_001794"
     CDS             complement(5056..6243)
                     /locus_tag="WM704_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HFA9341158.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            complement(6244..6528)
                     /locus_tag="WM704_001795"
     CDS             complement(6244..6528)
                     /locus_tag="WM704_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFA9341159.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            6714..7084
                     /locus_tag="WM704_001796"
                     /pseudo
     CDS             6714..7084
                     /locus_tag="WM704_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
BASE COUNT         1663 a         1775 c         1638 g         2008 t
ORIGIN      
        1 cgaaaagagt tcttaaaata gcccgaccgc cgccaagccg caaacttggc ggtttttttg
       61 ttgccctatg attttaaatc gagtaaaatg tatctacgat tattttcaac aagggggaag
      121 aatgaaccca accaaacaat ccaaaaaaag ctacgaatct aagcgcgttt taaagcacgt
      181 ttcatttaac actgaaaaag aagctaattt attggaattt tccaataatt tagacttctc
      241 caaatgggta aaagaaaagc tgaaacatga acttgaatta gaaaaactga aaaaataata
      301 gaaatggctt gcatgaatcg tagatacaaa ttataatttc aacacttgct gattgcaggt
      361 aaaaaagaag cccaaaccaa gctgtaactt gatttgggca ggtggaaagc agagcggcaa
      421 ctctctttcc gcgtctatct accatgaaat agaaaggtca ttctaccatg aatactgtcc
      481 aagttttaaa ctttcaacaa agctccgtcc gtgcttcccc ccaaaatatt gacgcaatag
      541 cggaataata gtaaagttcg gttcacaggt ttggtcgcct gtttctttcg tggttattgc
      601 cgcgcttgcg gcttttgttt tgataagagt tccctgaagg gtgtatgtta aacatacacc
      661 cttgctgaaa aagccaacgg gcggactttc gggacatctc cggatgtgct gtttccacga
      721 gcagtcgacc aacctgaaac gtccgcctac ctcttcttgg tcgaagagag gctttcaaac
      781 tttcgtggag cttaaaatgc aaaacaccat ttctgtattt tctttcaaat ctcaaaatgt
      841 ccgtactcaa atcttggggg ctgaaccttg gttttgcctt ggcgatgttg cggaaattct
      901 tcaaattcaa aatgcccgtc agttgccatt aaaagaccag ggcattcaaa aatccagcgt
      961 tgcaacaaaa aaaggcaacc aagaattact tttcatcaac gagccgaatc tctaccgagt
     1021 catcttccgt tcacgcaaag ccgaagccgt caaattccaa gactggatat ttgaagaggt
     1081 tatccctcaa atccgcaaaa caggcggcta ccaaattact cccaaaacca ccgccgacga
     1141 ccgtaccgga ttgcgccggg cggttgccgc gcttgtcggg cgcaaacgca taggctactc
     1201 ctccgcgtac agtatgatac accaacgctt caacgtcgaa gccgtcgaag gcatccccgc
     1261 cgacaaactg cccgaagccg tcgcctacgt ccacgcattg acactgcaca cgggtttggc
     1321 cggcgaagtc cccgaccgcg aaccgttgcc cgcgccgcaa cccgccctgc ccatcgacgg
     1381 caactcttta gccgacattg ccgctatggt ttattacggc acacggatga ttgaatcggg
     1441 caaagacgtt tccgcaccgc tcaaacagct tggcaacaga caggcggtta cgatgctgac
     1501 ggtctggcac gaaacccgtt caatcctgaa aagatccgcc gcggccctcg aagtgttgcg
     1561 gggatatgcg gacaaagacg cctccggccg catagccgca tgtcttgaag gcatttacgg
     1621 caaggctgcg gcaaggtaaa cggaaaatgc cgcctgaagg ttcggacggc atttttattg
     1681 aagggatgat tggggatttg taggcgtttg cagatacgac aaggggcgca ttccgcgccc
     1741 catcacacgc acggacggct tgtcatcgcc tgtcgcccgt gcgcggctgc ccatatttcg
     1801 gaacagtctt gcaagcccct ttaagggaac ggttttattc tagtacagtt tgaatgcctt
     1861 ggcaacggct gttttaaccg cctgaaaatc ctcttcactg attattggga tgcaacggtc
     1921 gcgccctttg ggtttgtatc ggtctaatcg tgccaatccg actgttgccg tcatatcgca
     1981 ttttgcccga cattggatgt gcggcttgtc cggtaagggg ttttcactca ttttgtggtg
     2041 gcagtccgcc aaagggacag gctctgtgct gcttaagggt acgaccgtta ccagtttgcc
     2101 gttgtgcctg tttcgcgcta tgacgacgac agggcgtttc ttgaccattt ccggttcttc
     2161 ataaccgcga aagtcgcaca tgataactga acgttccctt ggttggaatt ttaaaggcat
     2221 tagccgctcc tgacgatgac aggcggctgt tatggcagtt cttacacaaa aaaccgcttt
     2281 gtgtaaggcg gttgcaaaaa aaagccttcc aataaaaatg ccgcctgaac ctttcagagt
     2341 gcgaagcctg cccgcagggt gcggcatttt catttccgcc cattattggt gcgcctgata
     2401 attttgtata acgccccctt cgcgcaaaca aaacggcaaa aatttgccgc gtccgcccga
     2461 tatgtaaaaa atcgggggga atgccgacac ttcccccgat gcggccgcca accccgaacc
     2521 gacggttcgg ttgcaaactt tcggttggca actcaatccg cgcccaacgc ccgtaccagc
     2581 gcgccgtgcc gcgccttgca gtccttgtac aagccgatga cttgcaacga ccacggcagc
     2641 acgtccgcgc ccgtgttccc ttcaaggtgc ggcagtttcg ggcagggccg caccaaatcg
     2701 gcgggcggct taaccgccgt cgtcaacggc ggcgttgagt tcgcgcacgc cgtcagcatc
     2761 aaaacaggca ttgcggtaca caggtttttc aatgatttta agcgtttgca catagcgcac
     2821 cctttctttt tcctcacgcg ccgccttttg cgcctgatac gcggcggacg atttgcgggc
     2881 gtgttcggcg tgttcgaccg cggcggcttt gaggcgttcc gaaacctccg acaccgccgc
     2941 accgtatccg cgacggtatt gcgccgccct gtccagctgc caagaaacgg cgaagaacgc
     3001 gattgcggac aaaataagca gcggcttcca atttttcagc aaagccccaa tcatttacgc
     3061 ctcgcccgcg ccctgtttcg ccgtggcggc aacgtccgac aaccggtaac gcccttcggc
     3121 gggggacgaa ggcacaggtt tgccgccaat cagcttggac ggccagaagt agccgtcaat
     3181 gtccgcaggg tcaaacggga tgatggatac catattgccc tgattgccgc ccaagcccaa
     3241 gattctgcct tccgcgtctt tgccgacaac gaagaacacg tgtccgccgc cccggcgcgg
     3301 tttgaccgcg atgcagccgt atgcgggggc ttcgagtttc gtcaaacccg acattgacca
     3361 ggctttggcg cgataccagt ccctgatgac cgcgcgtccc gctttgccca ggcagtatcc
     3421 gacaaacagg ccgcaccacg gcgtttcgtc ttcaaaatac caagactttg ccgcgccggg
     3481 gaagccgccc gtctctttga gccattgcac aatcgtcgga ttgtgtttcg cgccgggaat
     3541 ttctttcaaa ccgatgtgcc ttctcgcttc ggctatccac ggtaattctg tcatttttaa
     3601 tcctttcggt aaaagacgag atgtaaactt tgggttggca actcgttcta tgttttaagt
     3661 cgcaatcaca atgtgcaccg catcgccgaa cacgctgtct ttttcagtcg cgtttgccga
     3721 tacggtaact ttccgcgcag atgatatgtt gcccgtcaac acaaaataaa aaacaaagtt
     3781 gcaacatgct gatttatatt gttattttta tttacgttta tttacgatat gcaaatgcac
     3841 ggttacaaag atatatttgc ataactgttt aattttattg aattttattg attcaatcag
     3901 tgtctttccg catcgtaagg ctgcccggtt ttaacaatgt aataggcggg cttcgccagt
     3961 ttgcgcatga tggcaacgat aattaccatc tttggcttac ccgctttttt cagattattt
     4021 attaatttcg gaaatgcgtt aaaacggtaa gcacaaaggg cgggcatata cagcgtactt
     4081 tttaatcgtc tgtttccgta tcggctcaat ctgccccgac ctcttacgct tgtccctgat
     4141 tgtatgatgg cgggacttaa tccggcatag gatacaaatt ggtttgcggt tttaaaatgt
     4201 ttttctgtca gttgcgcata aagaactgat gcggtgtctt tgcctatgct cgggatggtt
     4261 tgaagattgc ggtaatggtt attgtccgtt tgttttttga tttgttcgga tatggctatt
     4321 tttacctgtt ccatcttgtc ctgtatggta tctatcaagt cttgatgtat gttccttatg
     4381 aagtcttctt cagtgctatg aagacggttt ttaatttgct tctgctgttg ctgtaattgg
     4441 tttttaagat taatcagttt ttgcagtgct ttgtttttgg gtatctgata cggtatcaat
     4501 gtatcttgat gcctttttat gtaatctgct atcaggtttg aatctgcttt gtcggttttg
     4561 gtacggttaa acctgctttt tccgtagtca ttgattttta agggattgat aacgtaaacg
     4621 gtatagtagg aagaaagcat atctgctgcc ttttcgtaat agatgcctgt tgcctccatg
     4681 ccgatataga cttttctgat tctgtttccc tttatccaca atctaaactg ttttaatcca
     4741 tcatcattat tcttaaattt aatgtaatgg atacttccgt ttgttttatg caatgttgcg
     4801 tctatggtgt cctttgagat gtccggcccg attatattca ttggtatttt ccttatttat
     4861 acagccttga tacggctagg atgatattca attttgagga tggataaagg cagccggcat
     4921 ttctacgcgt ctgttttaat acattgcggg atttgctgcc tgactgcctt agcccttgct
     4981 ttgcgcgaaa caaagacccg taagccgtct atattcaaac ggtttacggg tcttttttct
     5041 ctgttgccgt tttcttcagt ttgccgatcc gaccacgccc ccgccgattc cttcaaacgg
     5101 tttcccgcgt tcttcccaat tgtcgtacat taggttctgc tgcggttttc cgcccaaggt
     5161 ggcaacttgc gccctgtccg aatgttgctg cgcgctttgc tgaacttcct gcccttggct
     5221 ttcttctttg tatgggttaa acggcaagcc gttttttaca tagtccttgc acatcaactc
     5281 cgtcacttct ttcaatgccg tcccttgatg cgaatagcag gtgcatccgg ttcttccgcc
     5341 ttctatacag cctgctatat attcaaaggt ccttacctgc cttacaccgt tataaatcgg
     5401 cttgctttcg ggtttttcgg gcaatgtcgg aacaaacata tctgccgtaa ggtttccgtt
     5461 attcaccgat tctccttctg ttttatccgg aagtactgcc tgctgttctg ttgccgccga
     5521 ttcttgtgct gcgggttctt cctgtttttt tccgtaactg cccaacattt tgtaagacaa
     5581 accgacaaat agcggaatca ataatatgat gacgggcaat gcataaaacc attttgaacg
     5641 cttgactttg tttaccgtgt gaatttctgc ggattcgtac aagtcataaa cttttttatc
     5701 cagtgtgtag atactggaaa atgcacttga tgccattttt accgggtcat ccgcgcatac
     5761 tttccattca agcagggtac gcaaacccat tttgttggcc gcaatgtggt aatgtctttt
     5821 aaccaatgtt cgcaagttct gatctaagag tttaggacct tgtgtcaata caaatatatc
     5881 tatgccctga tgcctgtgtg tgttcagcca ttggacgttt tcggggattt tcgaacctgc
     5941 ggagcgtgcg ggccatacgt cttgcgcctc atcgacaata acgattgcgc cgacgttttc
     6001 aggcttcttg atccattcat acatatcatg cgccgaaagc tgttcatcgg ttgatttcgg
     6061 cagcttcttt gcgtctgttt ctatgtgggt gtgcggtatc ttcaaacctt tgatgttcgt
     6121 aaatacttta cggcgtacgc cgttttcatc tggcttaaac atttcatcgt ttgccatcat
     6181 ggaaaccatt tttaatgttt tccctgaacc gggcgtgccg gttatcaaac agatttctgc
     6241 catttatttt ttcttcccga ttgaggttgc gagttttgtc atttgtttaa aggacagaat
     6301 aaaggcgatt gcgccgaaaa gaatgtttag aacagtacca ccgccgctta tatagaaaag
     6361 ctgtaacatt gcttgaggcg cgcccgttat gctatgggtt atcgcctgtt gaaaatgggc
     6421 tgccaatcta tctacacccg cataggtaac agccatcaag cctaatgcag tcaatatacg
     6481 gcctgcgacg ctcatcaaga gcggaatcaa tgcggccaac aatttcattt gctctccctt
     6541 tcttaaaagg cacgtttgcc tcattaaaca aatgtttctt aatctgaaag attgctccgc
     6601 attcgcggcg cggcagcgct gtggggacac cccctcgcgc ttatcgccaa tccccccccg
     6661 cgcttctcgg ctgtttgcgg cttcgcccgc aaagtgcgat gcttcgcctg cctttggcta
     6721 aagttgctta attggattgg aataattttg ttttaaatcg ttgcacattt gattggaacg
     6781 gcggaggttg tgcggtcaat aaaggtgatg atttcagagc tggggcttct ttttcccttg
     6841 gccgcaatcc gaaatacaaa gaagaaatgg atgccaaaaa gccggaagag attttatcgt
     6901 tgaaagtcga tgccgatccc gacaaataca taaaggcaac cggatatccc ggttattccg
     6961 aaaaagtaga agtcgcaccc ggaacaaaag tgaatatggg gcccgtcacg gacaggaacg
     7021 ggaatcccgt tcaagttgcc gcaacattcg gcagggacgc gcaaggcaac accacggcgg
     7081 atgt
//
LOCUS       DAWTHD010000104         7007 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWTHD010000104 DAWTHD010000000
VERSION     DAWTHD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.104"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(19..1785)
                     /locus_tag="WM704_001797"
     CDS             complement(19..1785)
                     /locus_tag="WM704_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFA9341160.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(1778..2659)
                     /locus_tag="WM704_001798"
                     /pseudo
     CDS             complement(1778..2659)
                     /locus_tag="WM704_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2696..2980)
                     /locus_tag="WM704_001799"
     CDS             complement(2696..2980)
                     /locus_tag="WM704_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9341161.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(2967..3197)
                     /locus_tag="WM704_001800"
     CDS             complement(2967..3197)
                     /locus_tag="WM704_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFA9341162.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            3469..3849
                     /locus_tag="WM704_001801"
     CDS             3469..3849
                     /locus_tag="WM704_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9341163.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4083..5123
                     /locus_tag="WM704_001802"
     CDS             4083..5123
                     /locus_tag="WM704_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFA9341164.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5120..5494
                     /locus_tag="WM704_001803"
     CDS             5120..5494
                     /locus_tag="WM704_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341165.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5491..6129
                     /locus_tag="WM704_001804"
     CDS             5491..6129
                     /locus_tag="WM704_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341166.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6249..6536
                     /locus_tag="WM704_001805"
     CDS             6249..6536
                     /locus_tag="WM704_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341167.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6610..6831
                     /locus_tag="WM704_001806"
     CDS             6610..6831
                     /locus_tag="WM704_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341168.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6824..>7007
                     /locus_tag="WM704_001807"
     CDS             6824..>7007
                     /locus_tag="WM704_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFA9341169.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCH"
BASE COUNT         1724 a         1777 c         1635 g         1871 t
ORIGIN      
        1 gctgttttcg gttgaaacct aaccggtttt caaaagtttc agacggcatc ttgtccgggt
       61 gcgttcaaac gcctcttcca gcctgccctc ggtcacacct tccgggtagt gtttgaggat
      121 gaccgacact gcctgtttcc agtcgccgcc gtctgcctcg acgcggggtt ttaagcgttt
      181 ggcgatttcg accttgctca atacctgctc ggcgacctcc atcttgttgt aatccatctg
      241 ttgcccctgt ttgggcataa agagcgtatt gcttgcggca agcgtatctt cttgatgttt
      301 gtacgggtcg attttgccgc cgaacgggac tgccttgcct ttgcgtttgg cggctgccgc
      361 ctccagcgtt tccgcaccca tcgccagctt gtccagctct ttgcgatgcc gttgcgcgtc
      421 tgtatcggcc ggggctttgt attccgcccc gatgaccgcg gcatcagcct tgaagcccat
      481 ctcgtcaaaa actacttcgg gtacggatac ccaaacctcg ttaccctccg cgtcataagt
      541 ggcgacccgc gccccgtttg cctcccaagg attcttaccg accaaaactt tcttaccgac
      601 caaaatcccc ttgatgcctt ttacgctata tacccgtccg ccaaagcgga tttccaaatc
      661 cgccgagact ttcgcctctt tcggcgcgct gacggcaagc tctcggcaat attcggcagg
      721 cggcggcagg atgagctgct cgggtttgat tttgttccac gcctgatagc gggtcatgcc
      781 gtggcggctg tgtttttgcg taccgttgta gtaacgcatc cagcgttccg ataaagcatt
      841 gagctggtcg atgtcgtgta cctcggtaaa gcgcaacccg ctctcaaatg ccgtttccac
      901 aatatcgttg gctttttcca cttgcccctt ggcacgcgga ttgcccggct tgttgatttg
      961 cacatgcaca tccaagggct tgcacaaatt tttaaacgcc gccgaagtat tcgcgctgcc
     1021 cgggtcaagc atgaccatgc gcggtacggt ccggaacggg tctttgccga tgtctttttt
     1081 cgcctgcatc atgtagatga aaaaatcaca gaggttcgcg ctggtttcgc cgccgaagta
     1141 ataacgcacc gaaatcgtgc cggaggcatg gtctgtcccc gtgtaccgcc aaacgcggtc
     1201 gttttcgatt ttgacgacgt ttttcggctt gtttttataa aactcctctt ccttcatcac
     1261 ccgcagcccc gtatccttgc cctgacgcgg caggaaatac aaaacgcaca aactcgggtc
     1321 gatttgccaa caatggttcg ggtgttccga tttcatgcgg ctgacggggt cgggctgcaa
     1381 aagctggtcg gaatgcagct tgtattcccg taaagcccgg gtaatggtgt tttcagaaag
     1441 ggggatgact tccccggttt ccccgtcaat ccgcgccgcc tcgattttcc cgttggcgcg
     1501 cagcatttcc accgcctgcc gcaccggcat caaccgcttg ccgttgcgcc tcatcgcctc
     1561 cgccaaaacc gccgaaatca atttggcttc ttccggttta agctccgtct tgcccgcatc
     1621 gctgcgccgt ttgcgcgtcg gcttgacgct gaccgcctcc agcttgcggt atagcgtggc
     1681 aaggctgatg cccaattcct gcgcctgctg cttaagatat gcagagcgtg cgccgcgccc
     1741 cattgcttcc gcctgatttt cgactgcttt aagacgctca atcattgccg gattcatcgc
     1801 cttctcccgt ttcaccgccc aaccattccg gcacattgtc tgccggtgct tcggtcggta
     1861 gggcatagct ttcgcgcagt tgctcgcagt ccaaaataat ttgattgagc gtgccgacca
     1921 tcttcgcgcg gtggtcaaat ccatgcgcct cgccgtgcgc cgccatctgc tcgaacatat
     1981 cgcgcaatcg gctgatttgc gagcggatac cgacttccaa gctgtcaagc tgccaagctg
     2041 catcgtcagc tcgatgccca catccgcagg tttaggctct ttgacacccg tctgcttttt
     2101 cgacagcttt tccgccagct catcgacctt tttattttta tcggcaatca ccttatcttt
     2161 cgcttccgcc gtttcacggc tttcgctcag ggcgacgcgc agctccttaa tagtcatacg
     2221 gtcgacatcg tcaaaagtgt tgccgttgac ctcaccgcct tcggcaagct ccaacaaagt
     2281 gtcgtcgtct tcgaccagca gctcgagcag tttggatttg cccaacgtca tcagcttagg
     2341 ctgcgcttgt ttcattttcg ggtcgataaa acggagggtg gcattcatca gtctttggga
     2401 ttcccgccgt ccgaggccaa ctctttttca gcgatttcgg caaaccgccc atgcggcgta
     2461 tgctctttga tgatgatcag cgcgcgtccc ggctcaaaca tcccttccat cgtttggcgt
     2521 ccgcaaatcg tccgcgctca atccataccg tctcgttgta ggcctcgccg cctgaaaact
     2581 tgtccataac ctccatactg tggatagcca attcgtttgc cgttacgccg accgtatgtc
     2641 ctaaaatttc tgttgccatt tcttttgctc ctcaaatgcg acgtcgtcgt cgcatttagt
     2701 aaattcggtt ttccaattct tgcaagcgcg cattcagccg ctcttgctgt tttctgaacc
     2761 gctctgcgat ttgtagggtt ttgatgccgt aggcgtagtt gccgttttca agcttgatga
     2821 ccaatcccga tgcaaccaaa tcatcaatat ccctgctgac ttgtgatggc gtcagcccca
     2881 ggccgtctga aagctcctta ttgctgatgc cgattaccgg atgcgcgtcc atcgccaaaa
     2941 acacccgcaa cagccgctgc gcctttttac tcgtcgccat ccgcatcctc cttcagccca
     3001 aattttaccg ccgcttcgtg gcttttgccg aaattgcctt tcagcttgcc gcgcaagggg
     3061 tgttccaccg cggtgcgctc caaattgaaa tgtttcgccc ggtgcgtctt gcacacgccg
     3121 ttgcgcttaa accacacggc cgtgctgccg cgcgtttgcg gatagggaat aggcttgaaa
     3181 tttaaaggtt tttccatatt atttatgcct ttcgtgtggt ataatgtgtt tagttttagg
     3241 cctctttgca cgataaacat ccggtctgtt gtgcagcagc agggtcttgg cttgttttaa
     3301 aataaacaag ctttttcccg tagccgggtg tatgtgatcc atcatgagct gtcgcgcatg
     3361 agccttcaaa gtgttcattt tttcgtcctt tttcgtgatg atttagggtg tttcatgtct
     3421 tgatgtgtga attatgagaa aaatatttct cttttcaagg aaatttaagt ggaaaaaact
     3481 tctctttttg gtaagcgatt gaaagaagaa agaattaaat taggcttgaa ccaagcagaa
     3541 gctgctgaaa aatgtggttt ttctcgtgag atgtggggaa agtgggagcg tggtgaaaat
     3601 cgcccatcaa gtgaaaaatt attctctttt tctaaaatcg gcatcgatat tgattacgtc
     3661 atgcacggca gacgcggcga aacagccgcc atgccgtctg aatccttgag tgctgaagaa
     3721 aaagaactgc ttgccctgtt ccgtcagctc ggcagcggca gccgcaaaga gcttgctgac
     3781 tacgccgcct ttaagctggt ggtagagaaa aaagcccaaa ctgcgcttgg taaagtgagc
     3841 aacggataaa aaatgccgtc tgaagatttc agacggcatt tgggacactc tgacagtcgg
     3901 gctgctccgg tgttctgaaa cagatattaa gcaataggag cggccgtgca ttttaaagcg
     3961 tattaaaaac caccccgata ttcaggtcat cggccaagtg gttcagattt caaaagacct
     4021 taattaactt acaataggaa aaatgatgac agaaacgaat tactcttttg aagggctggt
     4081 aattgaaaaa aattattacc catcgtattt atccgaaaaa tgaaaataga gagcgtgtag
     4141 aacctaaaat cagtacacat ttgattgatt tgcctattac ggcaagaaga acattggaaa
     4201 ccaggctgac caaagctttg ggtaataaat ctcatggcat tgaaatgtct gttgtcaata
     4261 cggctgaaaa tagttttttt cagatagcgg cagccataca actcaaagag gaagcagagt
     4321 ttattgagga ttccgcacaa tttgcccaca tgttgaccga tgcacaactg aatacaaatg
     4381 caccgggtgg tattttgttg gttttaaaag gtagggttgg agataccggt aagccgtttt
     4441 tatgtgtaat taaggctgaa cctcaagatg ggttccgaac caaagaagag gatgacttta
     4501 tcacgattga attcttagaa gaattattac tgaccgattc agcaagatta ttcaagatag
     4561 gttttttggt ggctgaaaca gtaaggccgc tagagcaaat acaatctggg aattatcgag
     4621 cttttttgta tgaccatctg atgacacaaa cggaaactag accggcagct tcctatttct
     4681 atcaagtatt cttgggtatg agtatagctg cttcttcccg taaattgacg caggattttt
     4741 ttgagtggac acgcaatttt atcgataact ctgatttaag tgatgatgca aaattagatg
     4801 cgcatgaagc attgcgcgtt acattgaaaa gtgcggaagc aaccattagt gtaaataatt
     4861 ttgcccaaaa tcatttacct caagaaaaac gaacaactta tacagaattt atggtggaaa
     4921 aggattttcc tcaaaatgcc gtaagtaaag atattgaata tattaaaact cgtttacgca
     4981 aacggaggtc ttacggattt agtaacggtg tagttatctt gactcctccc gagcatactc
     5041 aggactatat ggaaattgcg ccaacggaag atggggaata tactgttgtc ctaattaaag
     5101 gacagttaca acaacaaaaa tgagtgatat tgatgatttc aaaagctatt tagaaagcca
     5161 ccaagcggca ttttccgctt ggggtagatt tgtggctgaa gaaattcaaa atcaattatc
     5221 caatgtcatt tctcctgttc cggttgccaa ttttctaaaa attgaagcaa agcctggagt
     5281 caaagagatt tcttcagctc tagccaagat tggccgaaaa aattacactt cacctcaaac
     5341 tcaaatgact gacttggtcg gcgtacgttt tgtggcattg cttgcggaac atattcaaat
     5401 agtttgtgaa atcattgaat cctcatctca gtggaatgct aaagtttcga aagattttgc
     5461 agatgaaatt cagcaaaatc ccaaagcaat ttgactatac aatccaaaca ttacgagatt
     5521 cgtcctaaac aagcattcat tacacccgaa aatgtcagca tccctgctga tttgtgctgt
     5581 gaagttcagg tgcgttcttt actccaacat gcttatgcag agctggttca tgacaatata
     5641 tacaagcctg acggcaatgt tccaaagcaa gcagagcgcg aagtcgcgaa gagcatggct
     5701 ttgatggaaa ctacagatga cttatttagt cgtactctgg caattttgaa agaagcaaat
     5761 caaccacaag aagagcttct tccccaatta tcacagcttt atcaaaagga aatagggctt
     5821 gttcctgaag ttgataaaaa aaccaatatg atttttctag aaacatttca atccagcatt
     5881 tcccaatcaa gtattctttc tgacatccgt tcacttttga atgagaagaa atatattgcc
     5941 aagcggataa aagaaaatgc tgaagaaatg tattttttca gtcagccagc agctttgttg
     6001 gtctattggc tgattgagaa agttggtgca gatgaagtat ggaaaaaatg gcctttgcct
     6061 gcatacaaca aaaacctgaa attcatttgt actgatttgg ataagcaacc atcccatgaa
     6121 ctcttttaac cccctttaaa agcccattca gacggccttt cctaattatg ccttaatcaa
     6181 acgcggcata gcggcagttc cgcagctggc ggaaatcaaa aaatccgcaa atcaggaagg
     6241 gggcggcaat gattgaattt gtccgagcca aaaaacggct gctttgggca tttgtacttt
     6301 tgcttgcgtg ggcgtgcggc taccggtacg ctgccgacaa ggccgaagcg gaacaaaccg
     6361 ccctgattgc cgcctatcgg cattcttcta tggttgcggc ggaacaatac gccttgcagc
     6421 ttaaaaaagc gcaggacgaa aggcagcggt ggtacgactt ttcccaaaaa caaagcacgg
     6481 atttggcggc cgccctgagc gaattggaca aaacgcgcaa caccttacag gagtaaaccc
     6541 gtgaagcggc agacaaagac ggcaaccgtt ttaacggcct tggcacgaac agcctgcacc
     6601 tctacaaccg tgccttcgga taccccgatt aaaaccgttg ccgtcgcgga aattccgccc
     6661 gttccttccg gactgctggt cgaatacgaa cgccccgagc gtccggccgg cggctctccc
     6721 gaacaacttt taaaccacgc cgtccgctat ggcggatact accgaaagct cgaaatccaa
     6781 attgagggct ggcagaactg gcacacgaaa ggccgtctga aacatgactg attttgccga
     6841 ccgcgcatcc gaacgcgaag ccgaatttct ggtggaagcc ttggcgaaac accaaccgcc
     6901 gtctgaaaac accgccggct tcagccattg cgaagactgc ggcgacccga taccggaagc
     6961 aaagcgaaaa gccgtccgag gctgtacgcg ctgtgttgtc tgccacg
//
LOCUS       DAWTHD010000105         6987 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWTHD010000105 DAWTHD010000000
VERSION     DAWTHD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.105"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            46..2187
                     /locus_tag="WM704_001808"
     CDS             46..2187
                     /locus_tag="WM704_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9341170.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVGDTKSRINITR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFEIKDKEKATNEEKKK
                     NRANEKIVYAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS
                     SLNPSFGVIWQPREHWNFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNT
                     EIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNTGYIKNHGYELGASY
                     RTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFKNPNLEIGW
                     RGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNNV
                     FDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2339..3304
                     /locus_tag="WM704_001809"
     CDS             2339..3304
                     /locus_tag="WM704_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9341171.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDPAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3322..3513
                     /locus_tag="WM704_001810"
     CDS             3322..3513
                     /locus_tag="WM704_001810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341172.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3532..4500
                     /locus_tag="WM704_001811"
     CDS             3532..4500
                     /locus_tag="WM704_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9341173.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGSSMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4490..5464
                     /locus_tag="WM704_001812"
     CDS             4490..5464
                     /locus_tag="WM704_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFA9341174.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPHMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5509..6135
                     /locus_tag="WM704_001813"
     CDS             5509..6135
                     /locus_tag="WM704_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341175.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6219..6977
                     /locus_tag="WM704_001814"
     CDS             6219..6977
                     /locus_tag="WM704_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9341176.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT         1610 a         2113 c         1816 g         1448 t
ORIGIN      
        1 acggtttggc ttaacttccc ttcatacact caaaaggacg aacaaatgaa cgccccgttt
       61 ttccgcctca gcctgctctc gctcacactt gccgccggct ttgcccacgc ggcagaaaat
      121 aatgccaatg tcgcattgga taccgttacc gtaaaaggcg accgccaagg cagcaaaatc
      181 cgtaccaaca tcgttacgct tcaacaaaaa gacgaaagca ccgcaaccga tatgcgcgaa
      241 ctcttaaaag aagagccctc catcgatttc ggcggcggca acggcacgtc ccaattcctg
      301 acgctgcgcg gtatgggtca gaactctgtc gacatcaagg tggacaacgc ctattccgac
      361 agccaaatcc tttaccacca aggcagattt attgtcgatc ccgctttggt taaagtcgtt
      421 tccgtacaga aaggcgcggg ttccgcctct gccggtatcg gcgcgaccaa cggcgcgatc
      481 atcgccaaaa ccgtcgatgc ccaagacctg ctcaaaggct tggataaaaa ctggggcgtg
      541 cgcctcaaca gcggctttgc cggcaacaac ggcgtaagct acggcgcaag cgtattcgga
      601 aaagagggca acttcgacgg cttgttctct tacaaccgca acgatgaaaa agattacgaa
      661 gccggcaaag gtttccgcaa tgtcaacggc ggcaaaaccg taccgtacag cgcgctggac
      721 aaacgcagct acctcgccaa aatcggaaca accttcggcg acggcgacca ccgcatcgtg
      781 ttgagccata tgaaagacca acaccggggc atccgcactg tgcgtgaaga gtttgccgtc
      841 ggcgacacaa aatcacggat aaatattaca cgccaagccc ctgcctaccg cgaaaccacc
      901 caatccaaca ccaatttggc gtacaccggc aaagatttgg gctttgtcga aaaactggat
      961 gccaacgcct atgtgttgga aaagaaacgc tattccgccg atgacaaaga taacggctac
     1021 gcaggcaatg taaaaggccc caaccatacc cgaattacca ctcggggtat gaacttcaac
     1081 ttcgacagcc gccttgccga acaaaccctg ttgaaatacg gcatcaacta ccgccatcag
     1141 gaaatcaaac cgcaagcgtt tttgaactcg gaatttgaaa taaaagataa agaaaaagca
     1201 actaatgaag agaaaaagaa gaaccgtgca aatgaaaaaa ttgtttacgc ctacaaactt
     1261 tccaacccga ccaaaaccga taccggcgcg tatatcgaag ccattcacga gattgacggc
     1321 tttaccctga ccggcgggct gcgttacgac cgcttcaagg taaaaaccca cgacggcaaa
     1381 accgtttcaa gcagcagcct caacccgagt ttcggcgtga tttggcagcc gcgcgaacac
     1441 tggaacttca gcgcgagcca caactacgcc agccgcagcc cgcgcctgta tgacgcgctg
     1501 caaacccacg gcaagcgcgg catcatctcg attgccgacg gcaccaaagc cgaacgcgcg
     1561 cgcaataccg aaatcggctt caactacaac gacggcacgt ttgccgcaaa cggcagctac
     1621 ttccggcaga ccatcaaaga cgcgcttgcc aatccgcaaa accgccacga ctccgtcgcc
     1681 gtccgcgaag ccgtcaacac cggctacatc aaaaaccacg gttacgaatt gggcgcgtcc
     1741 taccgcaccg gcggcctgac cgccaaagtc ggcgtaagcc gcagcaaacc gcgcttttac
     1801 gatacccatc ctaaaaaact gttgagcgcg aaccccgagt ttggcgcaca aaccggccgc
     1861 acttggacgg cctcccttgc ctaccgcttc aaaaacccga atctggaaat cggctggcgc
     1921 ggacgctatg ttcaaaaagc tacgggttcg atattggcgg cagggcaaaa agaccgcgac
     1981 ggcaaattgg aaaacgttgt acgccaaggt ttcggtgtga acgacgtctt cgccaactgg
     2041 aaaccattgg gcaaagacac gctcaatgtc aatctttcgg ttaacaacgt gttcgacaag
     2101 ttctactatc cgcacagcca acgctggacc aataccctgc cgggcgtggg acgtgatgta
     2161 cgcctgggcg tgaactacaa gttctaaaac gcacatcccg aaaaaatgcc gtctgaaagc
     2221 ctttcagacg gcatctgtcc tgataatttg atatagatta tcatttatcc tttctaaagc
     2281 cgttccggtt tgtccgaccg gcggctttgc cccaatatcc ccattttgga gacaccctat
     2341 gttgcgtttg accacattga ccctctgcac cgtcctcgcc ttgggcgcgt gttcgccgca
     2401 aaattccgac cctgccccac aagccaaaga acaggcggtt tccgccgcac aatccgaaag
     2461 cgcgtccgtt accgtcaaaa ccgcgcgcgg cgacgttcaa ataccgcaaa accccgaacg
     2521 catcgccgtt tacgatttgg gtatgctcga caccttgagc aaactgggcg tgaaaaccgg
     2581 tttgtccgtc gataaaaacc gcctgccgta tttagaggaa tatttcaaaa cgacaaaacc
     2641 tgccggaact ttgttcgagc cggattacga aacgctcaat gcctacaagc cgcagctcat
     2701 catcatcggc agccgcgccg ccaaggcgtt tgacaaattg aacgaaatcg cgccgaccat
     2761 cgagatgacc gccgacaccg ccaacctcaa agaaagtgcc aaagagcgca tcgacgcact
     2821 ggcgcaaatc ttcggcaaac aggcggaagc cgacaagctg aaggcggaaa tcgacgcgtc
     2881 ttttgaagcc gcgaaaaccg ccgcgcaagg caaaggcaaa ggtttggtgg ttttggtcaa
     2941 cggcggcaag atgtccgcct tcggcccgtc ttcacgactg ggcggctggc tgcacaaaga
     3001 catcggcgtt cccgctgtcg atgaatcaat taaagaaggc agccacggcc agcctatcag
     3061 cttcgaatac ttgaaagaga aaaatcccga ctggctgttt gtcctcgacc gcagcgcggc
     3121 catcggcgaa gagggtcagg cggcgaaaga cgtgttgaac aatccgctgg ttgccgaaac
     3181 gaccgcatgg aaaaaaggac aggtcgttta cctcgttccc gaaacttatt tggcagccgg
     3241 cggcgcgcaa gagctgctga acgcgagcaa acaggttgcg gatgcgttca acgccgcgaa
     3301 ataatgaaac ggcggcattc gatgccgtct gaaacacgga tgcaaaccgc ttcctgtgtt
     3361 tcagacggca ttgcccgata cgggcgttat cgcgggaatg gcggcaagcg ttttgattgg
     3421 aacttgcccc atcctctgcc cgtccggagg tttcaaacaa ggctttccgc tccgacggtt
     3481 cggactgcct tgtttgaatc tcctacgccg taacgctttt cccttctgtt tatgactgcc
     3541 aaaccttttt ccctcaacct ggccaacctc ctgctgccgg cggtgttgtt tgccgtcagc
     3601 ctgtcggtcg gcgttgccga tttccgctgg tcggatgtgt tttcgctgtc cgacagccaa
     3661 caggtcatgt tcatcagccg cctgccgcgc acgtttgcga ttgtgctgac gggctcgtcg
     3721 atggcggtgg cggggatgat tatgcagatt ctgatgcgca accgttttgt cgagccttct
     3781 atggcgggtg cgggccaaag tgcggctttg ggtttgcttc tgatgtccct gctgctgcct
     3841 gccgcgccgc tgccggtcaa aatgtcggtt gccgccgttg ccgcgctgat cgggatgttg
     3901 gtctttatgc tgctgatccg ccgcctgccg ccgacggcgc aactgatggt gccgctggtg
     3961 gggattattt tcggcggcgt ggttgaggcg gtggcgacgt ttatcgcgta taaaaacgaa
     4021 atgctgcaaa tgctcggcgt gtggcagcag ggcgattttt cgagcgtgct gctggggcgg
     4081 tacgagctgc tttggattac gggcggtttg gcggtgtttg cctacctgat tgccgaccgg
     4141 ctgacgattt tggggctggg cgagacggtc agcgtgaatt tgggtttgaa ccggacggcg
     4201 gtgttgtggt cgggtttgat tattgtggca ctgattacat cgctggtcat tgtaacggtc
     4261 ggcaatattc cgtttatcgg gctggtcgtg ccgaatatcg tcagccgcct gatgggcgac
     4321 aggctgcgcc aaagcctgcc tgcggtcgcc ctcttgggcg cgtctttggt tttattgtgc
     4381 gacattatcg gacgcgtgat tgtgtttccg tttgaaattc cggtctctac ggtttttggt
     4441 gtgttgggta cggctttgtt tttgtggctt ttattaagga aacctgccca tgccgtctga
     4501 aaaaaatatc ggttttatgg caggaagcag ccgtccgttg cgggtcgcct ttgcgctgtt
     4561 gctggtttcc tgcatcctgt ttatgacggt caacgtcaaa ggcgattggg actttgtctt
     4621 gcacctgcgc ctgaccaagc ttgccgcgct gctgatggtc gcctatgcgg tcggcgtgtc
     4681 cactcaactc ttccaaacgc tgaccaacaa cccgattctg accccttcga ttttgggttt
     4741 cgattcgctg tatgtgtttt tgcagacctt gctggtgttt acgttcggcg gcgtgggcta
     4801 tacatccctg ccgttgacgg gcaaattcgg ctttgaactg gttgttatga tgggcggctc
     4861 gctgctgctg ttttacacgc tcatccgtca gggcgggcgc gatttgccgc acatgatttt
     4921 aatcggcgtg attttcggga ttttgttccg cagcctttcc tcgctgcttt cgcgcatgat
     4981 agaccccgaa gaatttaccg ccgcgcaggc gaatatgttt gccggattca ataccgtccg
     5041 cagcgagctt ttaggcatag gcgcgctggt cctgctcgtc agcgcggcgg tcgtttggcg
     5101 cgaacgctac cgctcggacg tacacctttt ggggcgcgac caagccgtca atttgggcat
     5161 cagctacacg cgcaacacct tatggatact gctttggatt gccgcattgg tggcgacggc
     5221 gaccgccgtg gtcggcccgg taagcttttt cgggcttctc gccgcctcgc ttgccaacca
     5281 cttttccccg tccgtgcgcc attccgtccg cctgccgatg acggtttgcg tcggcggcat
     5341 cctcttggtc ggcggacaaa ccgtattcga acacttcttg ggcatgaagg cggtattaag
     5401 cgtggtggtc gaatttgcgg gcggactcgt tttcctctat ctcgttttaa aacacaaaaa
     5461 atgacggatg ccgtctgaac ggcagcccgc cccgaaagga caaaccatat gacgcaagaa
     5521 cgtttacccg aatttttcga ccgcgccccg acgctgaccg tacaagaccc gcttgccgca
     5581 ttcctcggcg cggccgaaaa cggcatcctc acttaccgct acgccgatgc cgtgcgcctg
     5641 tgcggacatt cctgcccgac cgtcgcgggc gcgtacctga tggttatcaa aggcctgaaa
     5701 gcactttacg gcgaagagct gcccgaacgc ggcggcatcg aagccgccat gcagggaacg
     5761 cgcgacgaag gcacggtcgg cgtaaccgcg tccgtcgtcc aactccttac cggcgcagcc
     5821 cccgaaaccg gcttcggagg catcggaatg cagggacgct tcgcccgccg caacctctta
     5881 tcctttggtg caggcgaaat caacggcaca ctgaccctgc gccgcaaaga caacggcaaa
     5941 accgttgccg tcagcctcaa cgccgccctg caaccctttg cacctcaaat gcgcgacatc
     6001 atgcccaaag ccgtcggcgg cagcgcaagc gcaaaagaac tcgaacgctt cggacaactt
     6061 tggcaggcac gcgttaaagc atttttgacc gaatcggcgg acgacccgca gttcgtcatc
     6121 gtccgcgaac tgtgaacgtt cagacggcat tccgaattcc aaatgccgtc cgaacccccg
     6181 ccaaacaaca acaaacctac gcccgacaag catccgccat gattaccatc cgcaacgtca
     6241 gctaccgcat cggcacacac cccatcctcg acaacgtcag cctcgacatc cccgaaggcg
     6301 gcattaccgc gctaatcggc cccaacggtg cgggcaaatc caccctgttt tcctttatgg
     6361 cgcggctgcg cccgcttgaa agcggcagca tcgcctaccg aggcaaaaac cttgccggca
     6421 cccccaccgc cgaactcgcc cgcacgctct ccatcctcac ccaagaaaac agcatcatga
     6481 gccgcatcac cgtgcgcgac ctgctgatgt tcggccgtta cccctaccat caaggcagac
     6541 cgactgccga atgccgccgt atcgttaacg gtgcaatcga agaattccac ctgcaggacc
     6601 tctccgaccg ctacctgacc gaactctccg gcggacaacg ccaacgcgcc atgattgcga
     6661 tggtgttctg ccaaagcacc gactacgtcc ttttggacga accgctgaac aacctcgata
     6721 tgtatcacgc ccgctcgctc atgcagattc tgcaaaagct cacgcacgaa cacaaacgca
     6781 ccaccgtcgc cgtattgcac gacatcaacc aggccgccgc ctacgccgac cacgtcgtcg
     6841 ccatgaaaaa cggcaaagcc gccctgaccg gcacacccga agaagtgttc accgtacaca
     6901 acatcaaaga actgttcgac atggacgtgg acgtactcga ctacgaaggc aaaaaactca
     6961 tcgtccacca catctgaagc tcaaaaa
//
LOCUS       DAWTHD010000106         6839 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWTHD010000106 DAWTHD010000000
VERSION     DAWTHD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.106"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            188..634
                     /gene="yciA"
                     /locus_tag="WM704_001815"
     CDS             188..634
                     /gene="yciA"
                     /locus_tag="WM704_001815"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFA9341177.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(683..1504)
                     /locus_tag="WM704_001816"
     CDS             complement(683..1504)
                     /locus_tag="WM704_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9341178.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1623..2081
                     /locus_tag="WM704_001817"
     CDS             1623..2081
                     /locus_tag="WM704_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFA9341179.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2224..3360)
                     /gene="lst"
                     /locus_tag="WM704_001818"
     CDS             complement(2224..3360)
                     /gene="lst"
                     /locus_tag="WM704_001818"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFA9341180.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            complement(3351..3641)
                     /locus_tag="WM704_001819"
     CDS             complement(3351..3641)
                     /locus_tag="WM704_001819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341181.1"
                     /translation="MAALFALCSILACLNAAAGKERGNNAAAGKEGGNGFPAFGGGVG
                     GVVPDVRRHIFGKINFNIRQLSEYLLKFRIFRTGTLGAYVQFVGMEFLGIWG"
     gene            3720..5945
                     /locus_tag="WM704_001820"
     CDS             3720..5945
                     /locus_tag="WM704_001820"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFA9341182.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAATLTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6242..6544
                     /locus_tag="WM704_001821"
     CDS             6242..6544
                     /locus_tag="WM704_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341183.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
BASE COUNT         1817 a         2029 c         1522 g         1471 t
ORIGIN      
        1 agggtaggat ttggcgggat tggtgggctg aagcccgccc tacgcatccg ccctacaata
       61 ctccttgcga atctgtcccg cgcccgacct tgtatgtctc aaatgggtta aactacgcac
      121 ttcccgactt cccgtttcgg gcaggggtgc aggtccgatt tatatttcaa taaaacaagg
      181 aaactttatg cagcacgaag aaggcaaccg ccaacgccct caaggcgaac ttctcctgcg
      241 taccgtcgct atgccgcgcg ataccaatcc caaccaagac attttcggcg gctggattat
      301 gtcgcaaatg gatttgggcg gcggcatatt ggcggcggaa atcgcgcggg ggcgcatcgt
      361 taccgtcgcc gttcaggaaa tgaacttcat ccgcccggtc aaggtcggca acgtcgtctg
      421 ctgctacggg cattgcgtcc gcgtgggcaa tacttccctc cagcttaaag tcgaagtctg
      481 ggtgaaaact ttgatgaacg attgcgttac cgaagaccgc catctcgtaa ccgaggcggt
      541 gttcacttat gtcgccatcg atgcggaagg caatccgcgc ccgattccga aagaaggcaa
      601 ccccaaattg tcgggcttat tgcccacccc gtaaaaatac ccgtaaatgc cgtctgaaac
      661 tgtcttcaga cggcattccg tatcatttga accaagacct gatgcgggaa aacagcgaca
      721 tactctgttt ttcaaagtct tcccgcactt cgggcacggc tgtttttatg accttcattc
      781 ctgcaaaagt ctgcgccaca ggcataattt caatcgtgtt cacattcata tgcgccggcc
      841 ggcggtacag ccacaatgcg gtttccgcaa tatcttcggg gcggataaat tccacaccct
      901 catacacgcc cgccaccctc tcgttatcgc ctttgaagcg cacgttggaa aactccgtat
      961 tgccgcacaa acccggctcg atattggtaa cgcggatgtt cttatccgcc agctctgcgc
     1021 gcaaattcag gctaaactgg cgcacaaacg ccttggtcgc cccgtaaacg ttgctgccgg
     1081 cataagcata attgcctgca atcgaaccca aattcatcac ataaccgccg ccgcgttcca
     1141 ccatttgcgg caaaatcttg cgcgtcagga acgtcaaacc caaaacattg gtttgaatca
     1201 ttgtttccca atcttcaaaa tccgccttgt ccgccgtgtc caaacccaaa gccagcccgg
     1261 cattgttgat gaggcagtcg atgtcggaaa attcatcggg gatgccgttc aaggcgtttt
     1321 ccaccgactc gcggcacgac acgtccattt ccaaagggta aaacaaagca cccaattcat
     1381 ccgccaaagc ctgaagcctg tccgcacggc gcgccgcacc gataacgcgg tatcccgccc
     1441 cgacaaacgt gcggcacatc gcttcgccga agcctgccga agcaccggta attaaaacag
     1501 ccattgtttt tcctttcttt ctcgtttccc aaacctgttt caggtatcat aacacccttc
     1561 agacggcatc gcgccggctg aaaacgacaa caaccgtacc accacgacag gacacattca
     1621 acatgaaaac ccaaatcagc cttgccgcaa ccgccctcgc cctattcctt tccgcctgcg
     1681 gaaacggcgg cgcgcccgca cagcccaaag gcgaaatttc cgaaaaccgc accgccgcgt
     1741 tcaaatctat gatgcccgac ttctcgcgta tgggcaaaat ggtcaaaggc gaagagcctt
     1801 acgatgtcga aaaattcaaa caggcggcgg cggcgtttgc cgaaagcagc aaaaaaccgt
     1861 tcacactttt tgagtccgat ccgcaaggca acggccgcgc cctgcctgcc gtttggtcgg
     1921 atggtgcaaa atttgaagcc gaaaaaacaa aattcgccgc cgccgtcgaa aaactcaacg
     1981 ccgccgccca aaccggcaaa ctggacgaaa tcaaagccgc ctacggcgaa accggcgcaa
     2041 gctgcaaatc ctgccacgac agtttccgcg cgcccgaata acatccgaaa cagaacaatg
     2101 ccgtctgaat ccttcagacg gcattttctt tgtcatttcg ccacgcgcaa agcaatcgga
     2161 aattatcttc aattcaaaaa atacgccata tcctgatttt tgccttactt actccgccat
     2221 tttctatttt gatttaccat gcgattgatt tttatcgtca aatgccaaaa tcggaatgtc
     2281 ggcctgacgg aacagcgcat aaacgggctt gagccaataa tcttccggaa gggaagccgg
     2341 tttcaatgcg taaacgtgca cattgggaaa atccttcatc gtcaacgccg caccgctgaa
     2401 aaaggtataa atttcatacc tcgtatgcgg gtttttctta atttcgcgca aaatatagtc
     2461 ttcgatgaca tagggcgaat ttaacgcggt tacgccggaa agcccgtagg tctggcgggg
     2521 atgcggcgcg acatattgta tgttgaagtt ttttgccgcc ttttccgaaa tttccttcat
     2581 ctctttgtcg ggcgaaccca aaagtatccg caccgtgccg cccgtttcgt cccccgcctt
     2641 cagttcggac gcatcgaaca gcggcaggta agtcatcttg cggcggccgt cgtccataat
     2701 gtttttcaaa cccttgaata tcgtgtaatg ctcgtcggaa gcattgcggg ttttggcgat
     2761 gctccaatcc ccgaccatca ttcgggcaaa attccgctta atcgccccgt ttacataaaa
     2821 ctcatcgccc aaatagctgc tctcccgtat caggttgttt gtgccgtcgt caaaggtctt
     2881 gatttccgca tccgggtaag tgctcaaaaa ggcggcgatg ctgacttttt ccaaactcgc
     2941 caaataaatc cgcctgacct tcggcagcag catcgacttc accttcagct ccgccatcgt
     3001 cggaatgaaa ttaaacgatt tgttcaaacc gtagggcagg tagaaaaaat acgcccgctc
     3061 cgccttatcc tttatctgat tgaaataata atcgtatttt tcattcctgt tttcagacat
     3121 cagcaccaca taaaaccgct cccccggatg ctgcgccata atcctttctg ccaccttcat
     3181 ctgcaatatg gtatagcaga aaatcagatt gacgggtttc ccctcttcat tgaagagttt
     3241 gtccttcagc agggaaaccg cgttcctttc cccctgattt acccggtcaa acgtataaaa
     3301 tatcccgaag caaaaaacaa tcaggcacaa cacggtcaaa cagacttttt tcaaccccat
     3361 atccctaaaa actccattcc gacaaattga acatacgccc cgagtgtccc ggtgcggaaa
     3421 atgcggaatt ttagcagata ttccgacagt tgccgaatat taaaattaat tttaccgaaa
     3481 atgtgccgcc gcacatcagg cacaacgccg ccgacgccgc cgccaaacgc gggaaagcca
     3541 tttccccctt cctttcccgc cgccgcgtta tttccccttt cctttcccgc cgccgcgttc
     3601 aaacacgcca agatcgaaca aagcgcgaac aaagccgcca aaatatgcca taatagccgc
     3661 gcacattccc cgcgcaatgt gtttcaaaag aaaaaaccaa cctacaagag aataatccta
     3721 tgactcaaaa atccaccatt gtttataccc ataccgacga agcccccgcg ctggcgaccc
     3781 aatcgctgct gccgattgtg caggcgtttg cccgccacgc cgatattgat gtcaaaactg
     3841 ccgacatttc cctgtccggc cgcattttgg cggcattccc cgaatatctg accgaagcgc
     3901 agcgcgtacc cgacgcgctt gccgaattgg gcgaactggt gaaacaaccc ggtgcaaacg
     3961 taatcaaact gccgaacatc agcgcatccg tacctcaact gaccgccgcg attaaagaat
     4021 tgcagtctaa aggctttgcc gttcccgact atcctgccga ccctcaaacc gatgaagaaa
     4081 aagccgtacg cgaacgctac gaccgcatca aaggcagcgc ggtaaaccct gtcctgcgcg
     4141 aaggcaactc cgaccgccgc gcgcctaaag cggtgaaaaa ctttgccaaa aaaaatccgc
     4201 acagcatggg cgcgtgggcg aaagactcca aaacccacgt tgccaccatg caaagcggcg
     4261 actttttcca taacgaacaa tccgttaccg tacccgatgc gacttccgta tccatcgtgt
     4321 tcaccgacaa acaaggcaat aaaaaagagc tgcgcgagcc tgttgccctg aaagccggcg
     4381 aaatcattga cgcggccgta atgagcaaaa aagccctgct cgccttcctt gccgaacaag
     4441 tgaaagacgc aaaagctaaa ggcgtattgt tctcgctgca tatgaaagcc acgatgatga
     4501 aagtgtccga cccgattatc ttcggacacg ccgtcaaagt gttcttcgcg cctgtatttg
     4561 aaaaattcgg cggcaaactg gctgccgcag gcgtcaacgt caacaacggc ttcggcaacc
     4621 tgattgccaa tctggacaag ctggatgcgg acacccgcgc cgccgtcgaa gctgaaatcg
     4681 ccgccgttta cgccgccaac cctgatttgg cgatggtcga ttccgacaaa ggcatcacca
     4741 acctgcacgt tcccagcgat gtcatcgttg atgcttctat gcctgcgatg atccgcaatt
     4801 ccggccgtat gtgggacaaa aacggcaaag cgcaagacac caaagccgtg attcccgaca
     4861 gcagctatgc cggtgtttac caagcaacca tcgatttctg ccgcgaacac ggcgcattcg
     4921 atccgacaac aatgggtact gtgcccaacg tcggactgat ggcgcaagcg gcggaagaat
     4981 acggctcaca caacaaaacc tttgaaatcg aagccgacgg tcaggttcaa gtgattgatg
     5041 cggcgggcaa tgtcctgatg cagcacgacg ttgaggcagg cggcatctgg cgcatgtgcc
     5101 aaaccaaaga cgctcccgtc aaagactggg tacaactcgc cgtcaaccgc gcccgcctga
     5161 gcaacacgcc cgccgtgttc tggctcgacg aaaaccgtcc gcacgacaag agtctgctcg
     5221 ccaaagtcaa agcctacctt gccgaattgg ataccaacgg cctcgacatc cgcgtcctcg
     5281 cgcccgaaga agccgctaaa ttcagcttgg gccgtctgaa aaacggcgaa gacaccattt
     5341 ccgtaaccgg caacgtcctg cgcgattatc tgaccgactt gttcccgatt ttggaactgg
     5401 gtaccagcgc gaaaatgctg tccatcgttc cattgatgaa cggcggcggt atgtttgaaa
     5461 ccggcgcggg cggttctgca ccgaaacacg tccaacagtt cctcgaagaa aaccacctgc
     5521 gttgggactc attgggcgaa ttcctcgcgc ttgccgtatc gtttgaacat ctggcgcaaa
     5581 aaaccggcaa cgccaaagcc caagtcctcg ccgacacttt ggatgccgcc accgaaaaac
     5641 tgctgttgaa cgacaaatcg cccaaacgca aagcgggcga actcgacaac cgcggcagcc
     5701 atttctacct caccctctac tgggcgcaag aattggcggc gcaagacaaa gatgccgaac
     5761 tgaaagccgc atttgctcca ttggcagcca ctttgactgc cgacgaagcc aaaatcgttg
     5821 aagaactctc tgccgtacaa ggcaaagcgg tcgacatcgg cggctactac gcagccaatc
     5881 ctgaaaaagc cgcacaagcg atgcgtccga gcgcaacctt taatcaagta ctaaacgcct
     5941 tataagcaca aaggcaaaaa tgccgtctga acattcgtcg ttcagacggc attagttatc
     6001 cctatctgcc tgattatgat taaacgccgc acccattgga tatatagtgg attaacaaaa
     6061 accagtacgg cgttgccccg ccttagctca aagagaacga ttctctaagg tgctgaagca
     6121 ccaagcgaac cggttccgta ctatttgtac tgtctgcggc ttcgtcacct tgtcctgatt
     6181 tttgttaatc cactataaat catataatct ttagtttttt accctacgga aagacaataa
     6241 tatgaaaact gttccaacca tcctgaccgg catccttttg gcaaccgccc ttcccgcatc
     6301 cgcacacggt atgcataaga gcaaacccct ggctatggac gagctgccgc cgatttgcca
     6361 acaatatttc aaacgcaccg aaacctgtta caacaaagcc ggaaacaaag cagatttcgc
     6421 acgcaacaac accaaattcc tattccaagc cctacctgcc gccgatttgg gacagcgtaa
     6481 gcaaatgtgc caaatcgcta tggattcgtt tgcagaaaaa acccgcaatc tgaattgcga
     6541 ataaagccgc atcagcaata gcaatgccgt ctgaaacaaa ggttcagacg gcattctcac
     6601 atcaaaacgt accggctgat aaaacccgcc ttccccgcat ccctgacccg aacaggatta
     6661 aacggagtaa cggtttcggc cattatggca agtccccatc ccaaaaatga ccatataaat
     6721 taataccata aaacattgat aaaaagcgtc tgccggttgc ggtttgttgc agggcgttac
     6781 ggaaaatcat cgtaaaccga tgatttttat atagtaattg acacaacctg aacaaaaaa
//
LOCUS       DAWTHD010000107         6756 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWTHD010000107 DAWTHD010000000
VERSION     DAWTHD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.107"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(169..825)
                     /locus_tag="WM704_001822"
     CDS             complement(169..825)
                     /locus_tag="WM704_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFA9341184.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            890..1780
                     /locus_tag="WM704_001823"
     CDS             890..1780
                     /locus_tag="WM704_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFA9341185.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            1897..3708
                     /gene="typA"
                     /locus_tag="WM704_001824"
     CDS             1897..3708
                     /gene="typA"
                     /locus_tag="WM704_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFA9341186.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(4105..4195)
                     /locus_tag="WM704_001825"
     tRNA            complement(4105..4195)
                     /locus_tag="WM704_001825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4159..4161),aa:Ser,seq:gga)
     gene            complement(4234..4320)
                     /locus_tag="WM704_001826"
     tRNA            complement(4234..4320)
                     /locus_tag="WM704_001826"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4284..4286),aa:Leu,seq:cag)
     gene            4409..>6756
                     /gene="rnr"
                     /locus_tag="WM704_001827"
     CDS             4409..>6756
                     /gene="rnr"
                     /locus_tag="WM704_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFA9341187.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPI"
BASE COUNT         1664 a         1728 c         1934 g         1430 t
ORIGIN      
        1 gtactgcctg cggcccgccg ccttgtcctg atttttgtta atccgctata taacggtttt
       61 ggacggaaaa acggcctgaa gccgtttcgg gcattcagac cgtttgtgcg gtggggaaaa
      121 tgccgtcaga aaggcagaaa agggcttcag acggcatgat gtcgggtttc aggacaggag
      181 caggatggcg gctgcggcaa gcgcggcaac cgataatgcg gcggcaagcg cggctttgcc
      241 ggcaaagcgg attgaggttt tgccttcgat gtatttgaag ccggttatca tcgggcggac
      301 gaggtttttc tttttgaata tgcggtatgc ggcgacggcg gcgatgtgga ctgcggaaaa
      361 aacggcgagc agcttgaaaa agttgaggtg gattttccgt ataaggctgc ccgtatgttc
      421 ggaaaccaaa tggttgaggt agccgttggt gctgaaggtg ttttcatcgg cggcaaaaag
      481 ccccgtgccg acttgaaatg agacggcggc caaaagcgca acgaccatca gtgcgcccaa
      541 ggggttgtgt ccgggctgga tatgttcggg aatgccgttt ttcagatagc cgcgtatacc
      601 tgcccaacct cggacgaaac gggagaaacg ggcggtatcg ctgccccaaa tgccccagca
      661 gaggcggaat acgagcagga aaaggacgag cagcccgacg cgcgtgtgcc attgcagcat
      721 atcgccgccg gcttttgcgc tataccacat aaagggcagg gatgcggcaa gcagccagtg
      781 gaaaaggcgg gtggggaagt cccagacttt ggttttgttt ttcataatcg gtttccggcg
      841 gtagaatcgg tttgttttcg agccttaatt ttaaccgatt ggaggggaaa tgtttcccgt
      901 tttccatctt tcaggcgaaa gccgccgccg gatgcttcag acggcattgc gttttcccca
      961 tgttttcaaa gcccgtgcgg aagattcgca caaagggact ttcggcacgc tcgccgtagt
     1021 cggcggatcg gcagggatga gcggcgcgcc cgtattggcg gcatcggcgg caatgtatct
     1081 cggctgcggt aaggtgcggg caggtttcaa tcaggatacg ctgccttttg ccgtcattgc
     1141 cggttttccc gagattatgt tggacacggc agacggtttg accaagcgtc aaggcataaa
     1201 cgcttggacg gcgggttgcg gcttgggtac agatgcggcg gcagtggaaa cggtggcggc
     1261 agtgctggcg cggaatcgag acgaagccgt cgttttggat gcggacgcgc tgaacatatt
     1321 atcaaccgat gccgaaaccc gaaatctggc gcgcgggtgt aaaaacctga ttttaacgcc
     1381 gcaccccgcc gaagccgcgc gcctgcttgg aacgacagtt gcacaggttc aggcggatcg
     1441 gacggcggca gtaaggaaga taggggcaat tctcggtgca accgtggttt taaaggggca
     1501 caaaacattg gttgccgcgt ccgatacgga aatctatgtc aacgaaagcg gcaatgcggg
     1561 attggcaacg gcgggcagtg gcgacgtatt gggcggcatc atcggcagtc tgctcgcaca
     1621 gggcgtgccg gtttttgaag ccgcctgcgc gggcgcgtgg ctgcacggcg cggcggcgga
     1681 tgttataaag gaatcggcag gcattgcggc tgggctgtcg gcaggggaaa tcgccccggc
     1741 ggcaaggtgg ctgcgcaact ggataactga aagtatgtaa gaagatgccg tctgaaaggc
     1801 aagggcttca gacggcatct tcattttcca aatactgtcc ggtaaatcgt ggtataatac
     1861 gcagttatct cttacagttt ttgaaaaaca ttaattatga aacaaatccg caacatcgcc
     1921 atcatcgcac acgtcgacca cggcaaaacc acattggtcg accaactgct gcgccaatcc
     1981 ggcacattcc gcgccaacca gcaggttgac gagcgcgtga tggacagcaa cgaccttgaa
     2041 aaagaacgcg gcatcaccat cctcgccaaa aacaccgcca tcgattacga aggctgccac
     2101 atcaatatcg tcgacacgcc gggacacgcc gacttcggcg gcgaagtgga gcgcgttttg
     2161 gggatggtgg attgcgtcgt cttgttggtg gacgcacagg aaggtccgat gccgcaaacc
     2221 cgtttcgtga ccaaaaaagc cttggctttg gggctgaaac cgattgtcgt catcaacaaa
     2281 atcgacaaac cgtccgcccg tccgagctgg gttatcgacc agactttcga gttgttcgac
     2341 aacttgggtg cgaccgacga gcagttggat ttcccgattg tttacgcttc aggtttgagc
     2401 ggctttgcca agctggaaga aaccgacgag agcagcgata tgcgcccgct gttcgacacc
     2461 atcctaaaat acacgcctgc accgagcggc agcgcggacg agccgctgca actgcaaatt
     2521 tcccaactcg actacgacaa ctacaccggc cgcctcggta tcggtcgtat cttgaacgga
     2581 cgcatcaaac ccggtcaaac cgttgccgtg atgaaccacg agcagcaaat cgcccaaggc
     2641 cgcatcaacc agcttttggg tttcaaaggc ttggaacgcg tgccgcttga agaagccgaa
     2701 gccggcgaca tcgtgattat ttccggtatc gaagacatcg gcatcggcgt aaccatcacc
     2761 gataaagaca accccaaagg cctgccgatg ttgagcgtgg acgaaccgac gctgacgatg
     2821 gactttatgg taaacaccag cccgctcgca ggtacagaag gcaaattcgt gaccagccgc
     2881 caaatccgcg accgcctgca aaaagaattg ctgaccaacg ttgccctgcg cgtggaagac
     2941 accgccgatg ccgacgtgtt ccgcgtatcc gggcgcggcg aactgcacct gacgattttg
     3001 ctggaaaata tgcgccgcga aggctacgaa ctcgccgtcg gcaagccgcg cgtcgtgtac
     3061 cgagacatcg acggtcaaaa atgcgaacct tatgaaaacc tgactgtgga cgtacccgac
     3121 gacaaccaag gcgcggtaat ggaagaactc ggccgccgcc gtggcgaact gaccaatatg
     3181 gaaagcgacg gcaacggacg cacccgcctc gaataccata ttccagcgcg cggcttgatc
     3241 ggtttccaag gcgaattcat gaccctgacg cgcggcgtcg ggctgatgag ccacgtgttc
     3301 gacgactacg cgcccgtcaa acccgatatg cccggccgcc acaacggcgt actggtgtcc
     3361 caagagcagg gcgaggcggt tgcttacgcc ttgtggaatc ttgaagaccg cggccgtatg
     3421 ttcgtatcgc ccaacgacaa aatctacgaa ggtatgatta tcggcatcca cagccgcgac
     3481 aacgatttgg tggtcaaccc gctcaaaggc aaaaaactca ccaatatccg tgccagcggt
     3541 accgacgaag cggtgcgcct gaccacgccg atcaaactga cgctggaagg cgcggtcgag
     3601 tttatcgacg atgacgagct ggtggaaatc acgccgcaat ccatccgcct gcgcaagcgt
     3661 tacctgagcg aattggaacg ccgccgtcat tttaaaaagc tggattaatg tttaaatgat
     3721 acgcgatacc gtctgaaaaa tttcagacgg tattttttat tcggacgggc tttgcggctt
     3781 cttgaagctg tttcagacgg cgtttttcct atccaatcaa gaaactgccg ccatttttcc
     3841 agcggcatat cgccccgtcg cgtttcggta tcgggttcgg tttcccggca gcatgtcgaa
     3901 caatatcgtt tgaagggata cgccttttga gtccttacag gcttaggaag aggataaaac
     3961 ataccgcaaa acataccata aaaaaacggt aacggatagt aatcaatggt aacgtagggt
     4021 aataggttgt agctgatttt acgcaataaa atcaatacgt aagtaacaaa aaatcccaaa
     4081 ctgtaaaagt ttgggatttg tacttggcag agaggaaggg attcgaaccc tcgatacgct
     4141 attcacgtat acacgctttc caggcgtgcg acttaaacca ctcatccacc tctctaatgg
     4201 cggaaattat cctaatcggg ataatttgtt atttggtgcc cgggagaaga ctcgaacttc
     4261 cacacccgtg aggataccag cacctgaagc tggcgcgtct accaattccg ccacccgggc
     4321 tttgctttat ttgctgtttt gcggtacagt gtcctgccgc aaagaagtcg ctattatata
     4381 gttcataaag agaatgtcaa cagtcaaaat gaataaaaat attaaatctt taaatttacg
     4441 ggaaaaagac ccgtttttaa gtcgtgaaaa acagcgttat gaacatcctt tgcccagtcg
     4501 ggaatggata atcgaattgt tggagcgcaa aggtgtgcct tcaaaaatcg aatcgcttgc
     4561 acgcgagctg tcgattacgg aagacgagta tgtctttttt gaacgccgtc tgaaggcgat
     4621 ggcgcgggac ggtcaggttt taatcaaccg ccgaggcgca gtttgcgcgg cggacaagct
     4681 ggatttggtc aaatgccgcg tcgaggcgca taaggacggt ttcggcttcg ccgtgccgct
     4741 catgccgatg gacgaagggg atttcgtttt atacgaacgc cagatgcgtg gtgtcatgca
     4801 cggcgacacc gttaccgtcc gtcctgcggg tatcgaccgc aggggccgcc gcgaagggac
     4861 ggttctggat attgtcgaac gcgcgcaaag caaagttgtc ggccgtttct atatggatag
     4921 gggcgtggcg attttggagc cggaagacaa gcgtctgaac caaagcatcg tgttggaacc
     4981 ggacggcgtg gcgcgtttca aacccgaatc cggtcaggtt atcgtcggca aaattgaggt
     5041 ttatcccgag caaaaccggc ctgcagtggc aaaaatcatt gaagttttgg gcgattatgc
     5101 cgacagcggg atggaaatcg aaattgccgt gcgcaagcat catttgccgc accgattcag
     5161 tgaagcgtgt gccaaatccg cgaaaaaaat tcccgaccat gtacgcaaaa gcgatttgaa
     5221 aggccgcgtc gatttgtgcg accttccttt ggtaacgata gacggcgaaa cggcgcgcga
     5281 tttcgacgac gcggtgtttg ccgaaaaagt cggacgcaat taccgtctgg tcgtggcgat
     5341 tgcggatgtc agccattatg tccgccctga cgatgcgatt gatgcagatg ctcaagaacg
     5401 cagtaccagc gtgtatttcc cgcgccgtat gattccgatg ctgccggaaa acctgtccaa
     5461 cggcatctgc tcgctcaatc ccgatgtcga gcgtttgtgt atggtgtgcg atatggtcgt
     5521 tacctatgcg ggcaatatca aagaataccg cttctatccc gccgtgatgc gctctcatgc
     5581 ccgcctgacc tacaaccaag tttggaaatg gctttcagac ggcatcggga atccgcacaa
     5641 agcccaaatc gacacgcttt acaagctgtt taaaattttg cagaaaaaac gtctggcgcg
     5701 cggggcggtg gagtttgaaa gcgtcgaaac ccagatgatt ttcgacgaca acggcaaaat
     5761 cgaaaaaatt gtccccgtcg tccgcaacga tgcccacaag ctgattgaag aatgtatgct
     5821 ggcggcgaat gtttgcgcgg cggattttct gttgaaaaac aaacatacgg ctttgttccg
     5881 caaccatttg ggccccacgc ccgaaaaact cgccaccctg cgcgagcagc tcggtctgtt
     5941 ggggcttcaa cttggcggcg gcgacaaccc gtcgccgaaa gactatgccg cgcttgccga
     6001 acaattcaaa ggcaggccgg atgccgaatt gctgcaagtc atgatgttgc gctccatgca
     6061 gcaggcggtt tacgaaccgc attgcgaagg gcatttcggt ttggcttatg aagcatacgc
     6121 ccactttacc tcgcccatcc gccgctatcc cgacctgacc gtccaccgtg ccatcaaagc
     6181 cgtattgaac cggaaaacct acacgccaaa caaaagctgg caggctttgg gcgtgcatac
     6241 ttcgttttgc gaacgccgtg ccgacgatgc tggccgcgat gtggaaaact ggctgaaaac
     6301 ttattatatg cgcgataagg tcggtgaaat atttgaaggc aaaatctccg gggtggcaaa
     6361 ttttggaata tttgtcactt tggacgatat ccatatcgac ggtctggtac atatcagcga
     6421 tttgggcgaa gattatttca acttccgccc cgaaatcatg gcaatcgaag gcgaacgcag
     6481 cggcatccgt ttcaatatgg gggacagggt tgccgtccgg gtcgcgcgtg ccgatttgga
     6541 tgatggaaaa atcgactttg tcctaattgc cggagaaagc ggcaggcggc ggaaggtcaa
     6601 attatccgca tctgccaaac cggcaggggc ggcggggaaa gggaaatcga aaaccaccgc
     6661 cgagaaaaaa acagcccgat gcggcaaagt aaggggaagg ggcgtgcctg ccgttgccga
     6721 atcggggaaa aaggcaaaga aaccggttcc gattaa
//
LOCUS       DAWTHD010000108         6669 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWTHD010000108 DAWTHD010000000
VERSION     DAWTHD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.108"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            105..1324
                     /locus_tag="WM704_001828"
                     /pseudo
     CDS             105..1324
                     /locus_tag="WM704_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(1384..2190)
                     /locus_tag="WM704_001829"
     CDS             complement(1384..2190)
                     /locus_tag="WM704_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFA9341188.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            2382..2591
                     /locus_tag="WM704_001830"
     CDS             2382..2591
                     /locus_tag="WM704_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFA9341189.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            2680..3894
                     /locus_tag="WM704_001831"
     CDS             2680..3894
                     /locus_tag="WM704_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFA9341190.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(3966..6545)
                     /gene="clpB"
                     /locus_tag="WM704_001832"
     CDS             complement(3966..6545)
                     /gene="clpB"
                     /locus_tag="WM704_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFA9341191.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
BASE COUNT         1488 a         1661 c         1778 g         1742 t
ORIGIN      
        1 tcgggcttat gtcgactaca tgctattacg ttttgtcggg aaattggtta tagtaatgag
       61 cgaaatgcac gcccctgaaa atctttttgc gaaaaggaag caaaatgtct gatgccgttg
      121 caaaagaaac cctcaatccg tttgaaatcg cgcgaaaaca ggtcaaaacc gcctgcgacc
      181 ggctgaaaac cgatccggcc gtttatgaaa tcctgaagag tccgacccgc gtgttggaag
      241 tcaaactcga cgacggcacg gtcaaaacct ttaccggtta ccgttcgcgt gcgttcccat
      301 cccggtgtga atctggacga agtcaaggca ctgtcgattt ggatgaccat caaatgctgc
      361 gttgcaggca ttccttacgg cggcggcaaa ggcggcgtta ccttggatcc gcgcgattat
      421 tccgaggcgg aactggaacg catcgcccga gcttatgccg aagcgattgc cccgctgatc
      481 ggcgaaaaaa atcgatattc ccgcccccga tgtgaacacc aacggcaaaa ttatgtcgtg
      541 gatggtggat gcctatgaaa acgtggtgaa acattccgcg ccgggcgtat ttaccggcaa
      601 accggttgag ttcggcggct ctttggcgcg taccgaggct accggttacg gcgtgaacct
      661 cgccgccgtc caagctttgg aaaaactggg caaagatgtg aaaggcgcaa cctacgccat
      721 tcaaggtttc ggcaatgtgg gctaccacac cggctattac gcgcatcaat ccggcgcgaa
      781 agtcgttacc gtatctactg ttgacgttgc tatctacaat gaaaacggct tggatatgga
      841 agcgctgttt aaagagttcc aagaaaaagg cttcatcacc aacaaagccg gttacggcaa
      901 agaaattacc aacgccgaac ttttggcttt ggatatggac gtactcgccc cttgcgcatt
      961 ggaaaaccag ctgacttccg aaaacgccgg taaagtccgc gcgaaaatcg tggttgaagg
     1021 cgcaaacggc ccgactacgc ccgaagccga cgttatcctg cgtcaaaacg gcgtattggt
     1081 cgtgcccgat attttggcaa actgcggcgg cgtggtcgtt tcctatttcg agtgggtgca
     1141 aaacctgcaa ggctattatt gggagtttga cgaagttcag gaaaaagaaa tcgtcgtcct
     1201 gcgccgcgcg ttccgcgata tttggaactt ggcgcaagag tatgatgtcg atttgcgtac
     1261 cgcgtcttat atgatgagta tccgccgtgt tgaaaaagcg atgaagctgc gcggctggta
     1321 ttgatgtttg aacaaaggat gccgtctgaa accatttcag acggcatttt tgtttggacg
     1381 gaattaatgg tgagaatggc cgatttggaa ggttaaagtt gtgtagttcg cctgtttttg
     1441 gcacaggctt tgatcgggga aatcggcttt gtattcgaca ctcgctttcc aaaagccttg
     1501 gcgcaagggg atgatgtcca cttcgccttt gccgtcggtg gtgtcggaga aggcttgggc
     1561 ttcggttttg tgcgttttgc tgcggtcgct ggtgtcgaag ccgtcaaatg tagcggtaac
     1621 ggtggcattg ggcagcggtt cgccacggaa cagaacgcgg actttgaagc gttcgcctac
     1681 gtgaatgttg gcgggattgt ccagcgggac gatttccaag ttttgtccga ccggtttggt
     1741 gatgatggcg gtgtccgcgc tttcgtgtcc cacgttgaca atgtttttac cgaacatacg
     1801 ggtttgttcg caatagcttg cgtcaggcat ttctttgatg ccagcctgtt tccagcctgc
     1861 tttgtttttt gaccggaaag taggctgata ttcggcggta acgaggtagc tgccgtcttt
     1921 gacgggacgg ttgctgcggt attggtagtt gtatgtgccg cgttgaatca tgttttcctt
     1981 acctttttcg gtaaccagct gcatcggttt gctgaagatg tgcaggcggt ctttggcgat
     2041 gggttcgagt tcggggaatt cgccgtagcc caagtcggct ttaaggtatt cgccgccgtg
     2101 cgtgtgggcg gtttcgaccc agacgcggtg ggcgtgtgcg gatgtggcaa atagtgcgga
     2161 aacggcgagc agtgtcaatg ttttcttcat ggtgcagcct ttatatcgtg cgggttaaaa
     2221 attttgttat gctataacaa aatatgtatc gtgtgaagac ggatttgccg cctgaaaact
     2281 gtctttttgt atcgatttga atttttcaga aaatgaagcg gacgtttggg cggaggcaac
     2341 ttgtttgata agatagcaat atttcaaaac ggagaaatat catgccttat gtcaatatta
     2401 aagtaaccgg cggcaaggaa gcaccgactg ccgcgcaaaa agcggaactg atcggcggcg
     2461 taatcgaatt gctggcacgc gtgctgggta aaaatcccga aacaacggtt gtcgtgattg
     2521 acgaagtgga taccgataac tgggatatag gcggcaaaag cgtcagcgaa cggcgcaaag
     2581 agggcaggta aaaagcctga aaaccccgcc ctgatgcttt aaatttcgcg tgaaaaagag
     2641 tacattccca cccattgccc aaaatttacg gaacacatta tggataaatt tcccaagtct
     2701 gcaaagctcg accacgtctg ctacgacata cgcgggccgg ttcacaaaaa agcccttcag
     2761 ttggaagagg agggcaacaa aatcctcaag ctcaatatcg gcaaccctgc gccgttcggc
     2821 tttgaagcac ctgatgaaat cttagtcgat gtcatccgca acctgccgac ttcgcaaggc
     2881 tattgcgatt cgaaggggtt gtattccgca cgcaaagcca ttgttcacta ctatcagaac
     2941 aaaggtttgc gcgatattac ggtaaacgat gtctatatcg gcaacggcgt gtccgagctg
     3001 attgcgatgt ctatgcaggc gttgctcaac gacggcgacg aaatcctgat tcccgcgccc
     3061 gactaccctt tgtggacggc ggcggcaacg cttgcgggcg gtacggtacg ccattatctg
     3121 tgcgacgaag aaaacggctg gtttcccaac cttgccgata tggaagccaa aatcacgtcc
     3181 aaaaccaaag ccatcgtcgt catcaacccc aataacccga caggagcggt gtacagcaag
     3241 gaaatcctgc tggaaattgc cgaactggcg cgcaagcatg gtttgattat tttcgccgac
     3301 gagatttacg acaaaatcct ttatgatggc gcggttcact gccacatcgc cgcgctcgcc
     3361 cccgaccttt tgacggtaac gttcaacggt ttgtccaaag cctaccgtgt agccgggttc
     3421 cgccaaggct ggatggtgct aaacgggccg aaacatcacg caaaaggtta tatcgagggt
     3481 ttggatatgc tctcgtccat gcgcttgtgt gccaatacgc cgatgcagca cgcgattcag
     3541 acggcattgg gcggttatca gagcattaac gaattcatct tgcccggcgg acggcttttg
     3601 gaacagcgca acagagcgtg ggaactggtc aaccagattc ccggcgtgtc ctgcgtcaaa
     3661 ccgatgggcg cgatgtatat gttcccgaaa atcgataccg aaatgtaccg catccgcgat
     3721 gatatgaaat tcgtttacga tttgctggtg cgcgaaaaag tcctgctggt acagggaacg
     3781 gggtttaatt ggatcaaacc cgaccatttc cgcattgtta cgctgcctta cgtccatcag
     3841 attgaagagg cgatgggcag gttggcaaga ttcctgcaaa cctaccatca gtaggattct
     3901 gataaaaaat gccgtctgga acggagattc ccgtttcaga cggcattttc aacagcagga
     3961 acaaatcagg caaatttcag tctgtcgccg tcggcttcta ccttgatttc gctttcgggc
     4021 gcatagtttc cggcaagcag ggctttggcc agcgggtttt cgatttccga ttggatcgcg
     4081 cgtttgagcg gacgtgcgcc gtaaatcggg tcgaaaccgg ctttggcgat gatgtccaga
     4141 gcggcatcgg aaacagtcag gcgcaggttt tgtttttcca aacgtttttc caaacctttg
     4201 agctggattt tcgcaatact gcggatattg gcctgatcca agccatggaa cacgaccact
     4261 tcgtcgatgc ggttgatcat ttccggacgg aaatgttctt tcacatcctc catcacaact
     4321 tctttcaccg cttcgtagtc ctgcgtgccc atttgttgga tgtgctggct gccgatgttg
     4381 gaagtcatca caatcacggt atttttaaaa tctaccgtcc tgccttgtcc gtcggtcaaa
     4441 cgaccgtcat ccaatacttg cagcaggatg ttgaatacgt cgggatgggc tttttccact
     4501 tcgtccaaaa gaatcacgct gtacggtttg cggcggactt gttcggtcag gtagccgccc
     4561 tcttcgtatc cgacataacc cggaggcgcg ccgattaagc gggcaacggc gtgtttttcc
     4621 atgtattcgg acatgtcgat gcggatgagg tggtcttcgc tgtcgaacag gaagcctgcc
     4681 aaagctttgc acaactcggt cttacccaca ccggtcgggc ctaagaacag gaagctgccg
     4741 taaggtttgt tcggatcggc aaggccggag cggctgcggc ggatggcatc ggacacggcg
     4801 cgcacggctt cgttttgacc gactacgcgg cggtgcaaca cttcttccat tttcaacaat
     4861 ttatcgcgtt cgccttccat cattttggat acgggaatgc cggtcatacg ggaaacgatt
     4921 tcggccactt catccgcgcc gacattatta cgcaagagtt tgtttgccgg ttttgtgctg
     4981 tccgtatctg cccgttcggc ggctgcacgc tgtttttcca aatgctccaa atcttcatac
     5041 atcaattttg aagccagtgc caaatcgcct tgccgttttg cctgttcgat tttaattttg
     5101 acttcgtcaa tttgtttctt aatattggca gcaccgtctg aaatcgcttt ttcggctttc
     5161 cagatttcgt ccaaatcggc gtattctttt tgcaagccgt cgatttcctc gtctatcagt
     5221 tccaaacgtt ttttgctggc atcgtctttt tctttttcaa cgtgcgcctt ttccatccga
     5281 agctgaatca aacggcggtc gattttgtcc atagcttccg gtttggtttc tttttccatc
     5341 ttgacacggc tggcagcttc gtcaatcaaa tcaatcgcct tgtcgggcaa aaagcggtcg
     5401 gtaatgtagc ggtcgctcaa ctccgctgcg gcaacaatgg cggggtcggt aatatcgatg
     5461 ccgtggtgga tttcatagcg ttcctgcaaa ccgcgcagga tagcgatggt gtcttccacg
     5521 ctgggttcgc cgaccaatac tttttggaaa cggcgttcga gtgccgcgtc tttttcgatg
     5581 tattggcggt attcgtccaa agtagtcgca ccgatacagt gcagttcgcc ccgcgccaag
     5641 gccggtttca acatattgcc cgcgtccatc gcgccgtcgg ttttgcccgc gccgaccaaa
     5701 gtatggattt catcgatgaa aatcagggtg ttgccgtcgt ctttcgccaa atcgttcaat
     5761 acgcctttca agcgttcttc aaattcgcca cggtatttcg caccggcaat caaagccgcc
     5821 aaatccaaaa ccagcaggcg tttgttacgc agggattcag gtacttcgcc gttgacgatg
     5881 cgttgcgcca aaccttcgac gatggcggtt ttacccacac cgggttcgcc aatcagcacg
     5941 gggttgtttt tggtgcggcg ttgcagcacc tgaatcgcgc ggcggatttc atcatcacga
     6001 ccgataacgg ggtcgagctt accgtctcgg gcgcgttggg tcagatcgag cgtgtatttt
     6061 ttcaaagcat cgcgttggtc ttcggcattc gcatcgttca cgttttgtcc gcctcgtacc
     6121 gcatcgatcg cggcattgat gttttgttcg gtcgcaccgg cttctttcaa gattttgccg
     6181 gccgcatcgt tctgctgcac caaggcgagc aggaaaagtt cgctggcgat ataggcatcg
     6241 ccgcgtttgg ttgccgcttt gtccatcagg ttcaacaccg cctgcaattc gcggctgggc
     6301 agaatctcac caccttgtcc ggacactttc ggcaggctgt tcaaatgctg ctgcaaacgc
     6361 tgtttaacct gcggcacgtt cacgcccgca tgagccaaga gcgcggcagc tccgctgttt
     6421 tggtcgtcaa gcagggcttt cagtacgaaa cctgcttcca gatagctgct gtccgcagcc
     6481 aacgccaaac tctgagcttc tgcaagggct tgttggaatt tggcggttaa tttgtcgtat
     6541 cgcattttcg tttccttttc aaaatgtccg ctgtcgaagc ctatatgtgc atgactgtgg
     6601 ataactcaag tgttgttttc tgtttttcta tatttaattc gatatattgt tgaatttaaa
     6661 gtatataga
//
LOCUS       DAWTHD010000109         6604 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWTHD010000109 DAWTHD010000000
VERSION     DAWTHD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.109"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..460)
                     /locus_tag="WM704_001833"
     CDS             complement(<1..460)
                     /locus_tag="WM704_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFA9341192.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLEKSQIKFQSSNNLKSI
                     GFKLDSSSNTFSKFTEAANFEHIPTGAKARINAKITASVSRAGVLSGVGKLVRQGAKF
                     GTRAVPYVGTALLAHDVYETFKEDIKARGCRYDPETDKFVKGYEYANCLWY"
     gene            complement(402..719)
                     /locus_tag="WM704_001834"
     CDS             complement(402..719)
                     /locus_tag="WM704_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFA9341193.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(847..1125)
                     /locus_tag="WM704_001835"
     CDS             complement(847..1125)
                     /locus_tag="WM704_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341194.1"
                     /translation="MYCQVGNKCLEKHRAENLYFSLVVPRIQENGQIIRPEYNGSMWK
                     MSDGQPLRLSLAECSPKDNLQSGLETGRIVFGVLASVYFVSLLKKVLK"
     gene            complement(1132..1416)
                     /locus_tag="WM704_001836"
     CDS             complement(1132..1416)
                     /locus_tag="WM704_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341195.1"
                     /translation="MFGGRLKNSPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAQANAALPETAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(1424..1621)
                     /locus_tag="WM704_001837"
     CDS             complement(1424..1621)
                     /locus_tag="WM704_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341196.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(1626..1916)
                     /locus_tag="WM704_001838"
     CDS             complement(1626..1916)
                     /locus_tag="WM704_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341197.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            complement(1961..3306)
                     /locus_tag="WM704_001839"
                     /pseudo
     CDS             complement(1961..3306)
                     /locus_tag="WM704_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(3445..3867)
                     /locus_tag="WM704_001840"
     CDS             complement(3445..3867)
                     /locus_tag="WM704_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFA9341198.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(3873..5516)
                     /locus_tag="WM704_001841"
                     /pseudo
     CDS             complement(3873..5516)
                     /locus_tag="WM704_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
     gene            complement(5530..5859)
                     /locus_tag="WM704_001842"
     CDS             complement(5530..5859)
                     /locus_tag="WM704_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9341199.1"
                     /translation="MAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSI
                     SNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRA
                     IVDNIEC"
     gene            complement(5898..6506)
                     /locus_tag="WM704_001843"
     CDS             complement(5898..6506)
                     /locus_tag="WM704_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9341200.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
BASE COUNT         1714 a         1505 c         1259 g         2126 t
ORIGIN      
        1 cgtaccaaag gcaattagca tattcgtagc cttttacaaa tttgtcggtt tcgggatcgt
       61 atcggcagcc tcgtgccttt atgtcttctt tgaaagtttc gtatacgtcg tgggctaaaa
      121 gggctgttcc gacataggga accgcccttg tgccgaattt cgcgccttgg cggacaagtt
      181 tgccgacccc cgacaatacg ccggcgcggg atacgctggc ggttattttg gcgttgattc
      241 gggcttttgc gcccgtgggg atgtgctcga aatttgccgc ttctgtgaat ttggaaaaag
      301 tgttagaact tgaatctaat ttaaaaccaa ttgattttaa attattactt gattgaaatt
      361 taatttgtga tttttctaat cttacaggtt cagaataaga attactcgaa catagaatta
      421 aaatcagaac tatcggtgta ctcttgataa acgaattcat gaacagtttt tccgttctct
      481 tctgattttc ttataaaaat aacagactca tcagaaaaat aaatcttcca aatattatgt
      541 gcgaccctct tatttaagaa ataagagaag ctctctaaaa tatcgtactc ttttacgtta
      601 ttaacaaatt cttcaaattc tttctgagca ataaaagcca tcgattgccc aaaatactcg
      661 cttgacggct gatatttata aagtgccaac tgcgcctgcg tgataaacgg cttgttcatg
      721 gttctgcctt tcaaaggttg ttttgaaagc ctgattttga caccataact tcatgcgctc
      781 aatccttaaa cagaaccgcc ccgattaata cggggacggc aacgccgaga tagaaataaa
      841 aatccatcat ttcaaaacct ttttcagcag ggaaacaaag taaacggacg cgaggacgcc
      901 gaatactatc cggcctgttt caagaccgct ttgcaggttg tctttcggac tgcattccgc
      961 caatgaaagc cttagcggct gaccgtccga catcttccac atgctgccgt tatattccgg
     1021 cctgattatc tgtccgtttt cttggattct tggtactacc aagctgaaat aaaggttttc
     1081 ggcccggtgc ttctcaagac atttatttcc gacttggcag tacatgccgc cttacttcat
     1141 caccctctta acgatggaaa atacaaaaag cgcggcgaaa acgcccacta caatccaacc
     1201 ggcttccata ccgtccgctt ttgcggcttc caaagcgttt tttgccgttt cgggcaacgc
     1261 tgcgtttgcc tgtgccgcca aagccagcgg ggcggctgtt acaacagcca gttttgcgcc
     1321 gtatttacgg caggtgttaa taaatttcat gatattttcc ttcaaaaagt gtttggcggt
     1381 aatggatgga gagtttttca gacgaccgcc gaacatccga aaatcagtct ttcaaaaatc
     1441 cgaatacgac aaattcgcat tggttgccga tttcttccaa acctgcgtta accgcttctt
     1501 cgaagtcgta gaaataatcg gcattggtaa ttaatttggt atgtccgatg tcgcccgttt
     1561 caggagagag atacagaaag tcccctgttg atacggactg gacaacatag actttctgca
     1621 ttcaatcagc ctttcttaac gaattgaaaa ccggtgactt tcagtttttg agttttgccc
     1681 gtagtgacga tttccacgtt caggtttgct tcgatcggaa attgggcgtt tcggaactgc
     1741 tcgaaattgg cagagccgcc gaaatcgtat tcagtagtag agctgcccaa tgcgttgcct
     1801 tgggagctgt ctaagggtgt ggcgacaatc aggcggcaat agtcgaagct cttgccttcg
     1861 atttgtccgt tgaatttttt aacgccgacg atgtggcctt gaagttggat gttcattttt
     1921 tggtttcctt gtgtgattaa acgtctttcg ggcagacact ttaagcccat gaaatcggta
     1981 gtcttgcgaa tttgtcgtaa atgaagttgt tatagctttc ttcattgttg acgtgttttt
     2041 gctgttcaag ctgtttttca agattctcgt aatattcgta catataataa gggtcattgt
     2101 acggtttgaa tgcgggctgt tcatgaatga cttgagattt caaaaaggcg cagtcgtagg
     2161 cttcgagagc caaagacttg ggcagcttgt gatgactcgg ctcaatcagt tcaaacagtt
     2221 tggctttgtc caattcggga aaaatgaatt tcagaccgtt tgccgcacgt ccgaactgtt
     2281 tttttaccca ttcaaggcag cggtcggctg aaacaacctt atcttcctta accgcgtgta
     2341 tgcgcgttgc cttttgggcg aatcgttcgc aaatgggata tgcgccgccg aaatattcgc
     2401 ccggattctg caaaacttcg aaagggataa cgatgtcttt tgctttgaat tcaatttcaa
     2461 agcgcgtcca tgtgcttgtt ttatcgccca actgcttgcc tttttcatag acgcggacat
     2521 atttggacga ttcacgggag ccgataccat aggtcttgcc tttggtcatt ttggcttcat
     2581 cgtcttcttc ccaatccgac cccaaacatt cgcccttcgg tttgacgtga tggcacgtaa
     2641 acaaaccttt attgcggtct tctcttgcct gattcgggct gtactctccg ttgaaaaagt
     2701 cttttgcaat gtcaacgcgc gttatttttg gacgaatcgc atttgtcaga aaatcaaaaa
     2761 gccttgattc ccatccaata tttgcagcat tgcagcctac cgccgtcaat tcaaccaaca
     2821 tcgtttcacg ttggccgccg taatggacgc gcccgtattg ggcattttcc gttcccatca
     2881 gccagcagcc ttcatagaaa cgcccgcccg aatgcttggc tttttcaatg attccgaatc
     2941 cgaaaatttc ctccatcttg gcggaagccg cgcgtatgaa atcgtcatca tcgaacaaat
     3001 caaacgaaag tccataaaca tggaaaaacg tgtcttcatg aattgaaaat gtgatttggt
     3061 caatgaaagc cgaatctgat acaccgcgcc taagaggaac gcctaacagg tttcctttac
     3121 cgtccgttat gtacgtttcg taacattcga agacttcctg aaccctgccc gccgtttcgg
     3181 tttctgtgcc ccccctgtta gataaggggg gggaagattc gaagcggttg ccggcttcct
     3241 gccgtccgcc ggcgcctccg tcatcacgcc ggcaacctcc tttgtcatcc cttgcttatc
     3301 ttccatttgc gaatcctcaa aaacgggcaa aaaaaaagcc ctgttacttg tagaaagtaa
     3361 agggcgttaa tttttgttaa tcgccccttc ttagggacgc aatatataag ggtcttaatt
     3421 aaaggaagat gccgtctgat tttttcagac ggcatctttt atttcctcga ccaaccgatt
     3481 taatcgctcc ctacaaatag ctttattttt taattcgcac tcccaaacga caatcacttt
     3541 gaaaccaatc ttttccagct cggtttcatt tttaatatca cgttcgcgat tttttgtgat
     3601 tttttccaac caaaaatcca tattgctttt cgggatatgt cctttattac aagaatgccc
     3661 gtgccaaaaa cagccgtgta taaacacgac tgttttatat ttaggcaaaa cgatatccgg
     3721 tttgcctgca tagcgcttat cgtttttccg ataacggaaa ccttgggaaa aaagaaattt
     3781 ccttaccaat acttccggtt tggtttcttt gctatggatt tttgacatca cgaaggaacg
     3841 tttggatgga gtgaaaatat cggtcataaa atttaaggcg atgtaggttt agctcgtttt
     3901 tttgtttgtt ccacttttct caataaatat gaaactaagc tgattaaatc caaagcatcc
     3961 ttgtcattaa aaatactagg agataaattt gctcttaact catgttgcac aggatttcta
     4021 aatccagtcc atgttccgca tgcaagctgt tcaagaccat cttctaaatt ttgttccgcc
     4081 tgcgttttgt tattcgttaa tagaagcata gattttttac tcccgaaggc tttttcaatc
     4141 aattctcttc cgtctatgct atgaagacct gtctgcttct gaaccttatc ttcgaaaact
     4201 ttaactgcct cgattgcagc acttaaataa tccttctttt cattgtaaaa atcgttacag
     4261 gcagtatgca aatcctgatg caaatgccgc caatgatagt aaggatattc tggaataata
     4321 ctatgtaagg cagagattac ttccgcttgt tctttatttg taagctccga atcttccaaa
     4381 cgattcaaaa taacttctac cggttctctt tccttttctg gcaatttgtt tttccaattt
     4441 tcgatatcaa ctccgctttc acctttcact tcgtaatctt tctctttttt tcttcttttt
     4501 ctccaatctt gcccgatggc tcagacgatt tccccaagga aaacccgtaa ttttgaagtt
     4561 ttaggatgct tccaatccaa tgatgtccta tctgtagcga ttaaatcttc accatcatta
     4621 tctataaaat caatacttaa ccatcctgtc agataggatt agaaatggct ggattcgcta
     4681 tcagtgaaaa actcgggcat atttaccatt ctgccgttgg caaacaaggt aatacctttc
     4741 atattattct ttaaaggttt ttccgttgta atgaacttac cttgtattaa tccgctgtat
     4801 tcatactgtt cgaattttga agataacgat gaaatattgg ttgctagatc ctggtattcc
     4861 cattcgaatt gtggggtaac aagattatat tttaggtttc cgtcaatgat tcttggttcc
     4921 gagccgttca agcttacttt gattttaaaa tcctgagccg gaaaatcaaa taaacgggaa
     4981 agatgcccca cataattatc taacgggtat ccttgttttt tcgtcagctc ggttaaagtt
     5041 atagttgttc cgttttcggt attgggttta acagactctt tctgaaactc cggttgataa
     5101 atacgctcac tttttttaat ctctgcataa tccaaagtaa aagtaacccg ttcgtttcct
     5161 tggatagtag agatttcgat tttgttgcca agcctgaata atgccaattt accaagacct
     5221 tttttacccg ttggaattct tccgcacggg gaggcttgtt tttcttccct tctgttccga
     5281 ccgattctca aataaaaatc attgatttca tcgaagctca ttcctatgcc attatcttta
     5341 ataacgattt tatgctccgg tttatcgaat aacctaactt ccacttccgt agcacatgca
     5401 tcgtaggcat tcgctatcaa ctcggcaatc gcaggaacag tgtgcgaata catctttaca
     5461 cccaaatgtt cgattacggt tggctcgaaa ctcattttca atttttcttc agacatgttt
     5521 tctccttttc taacattcga tattatcaac aattgccctg cctatttttt ccgcatacat
     5581 cggaggaacg gcattaccaa tcaaacgggc tatcttgtcc ctactgcccg ctttaaagac
     5641 atagttacga gggaacgatt gcaatgttgc accttctcgc agggataacg ccctatcttc
     5701 ttcaggatgg gcaaaccgcc cgttggaaat gctaaagaat ttggtcgtta tggtaggtgc
     5761 gggcttatcc caccatagcc taccgaacgt gtctttaaaa ctattgtctt ttccgataaa
     5821 acattccagc tgtaattcgg gaatatgcgc aaaagccaaa cggtttcctc cgtttttagg
     5881 tatcaaagcc aatcgtttca aattgatatc ggataatccc gcacaactat gcataaaatc
     5941 cgtctcgtct tgatgtcctg ccataatttt gggaaagccg ttttccattc ccaaaacatc
     6001 gcgtaccgta agccgtttgc ccgaatactt gactggttcc agcttttttt ggttattctg
     6061 tttgcaatta acgtaaatct tttacggctt tggggaattc cgtaatcagc cgtattgtga
     6121 ataccgaaat ggacggtaaa tcctttttct tccaataact ttatgaaatt atcaagcccg
     6181 ctttctttca ttctgctcag aattcccggt acgttctcta caacaacata tccgggattg
     6241 aaatattcta caaaccgctg aaactccaaa agcaaactct tggatttttc cgattttctt
     6301 ttgtctgttt gaataacact ccaatattga caaggactac aacctataag aatcaaatca
     6361 tcatcattct tttttaagcc gagttctttt tccaaggttt caggctgcaa ttcaaaaata
     6421 tcagcctgta taaatttggc atttatattg gcttcatatg tttctttgca gctaagttcg
     6481 taatctatcc cggctaaaac ctgaataccg gcggattaca ttccatagct cattccgcct
     6541 ccgccacaga aaaaatcaac cgcctttaac ttactgtttt tcatttctat ttaaataatt
     6601 agac
//
LOCUS       DAWTHD010000110         6271 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWTHD010000110 DAWTHD010000000
VERSION     DAWTHD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.110"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(71..1157)
                     /locus_tag="WM704_001844"
                     /pseudo
     CDS             complement(71..1157)
                     /locus_tag="WM704_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
     gene            complement(1094..1309)
                     /locus_tag="WM704_001845"
     CDS             complement(1094..1309)
                     /locus_tag="WM704_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFA9341201.1"
                     /translation="MSTQFDVAVIGAGPAGSAASALLRKKGYQVCVLENSTFRASSSA
                     KACCRTVWKCWKKPVLPMPFAPGPAFS"
     gene            complement(1489..2862)
                     /locus_tag="WM704_001846"
     CDS             complement(1489..2862)
                     /locus_tag="WM704_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9341202.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3076..4080
                     /gene="dusB"
                     /locus_tag="WM704_001847"
     CDS             3076..4080
                     /gene="dusB"
                     /locus_tag="WM704_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFA9341203.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4093..4242
                     /locus_tag="WM704_001848"
     CDS             4093..4242
                     /locus_tag="WM704_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341204.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4269..4508
                     /locus_tag="WM704_001849"
     CDS             4269..4508
                     /locus_tag="WM704_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFA9341205.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGDNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4511..5047
                     /gene="ruvC"
                     /locus_tag="WM704_001850"
     CDS             4511..5047
                     /gene="ruvC"
                     /locus_tag="WM704_001850"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFA9341206.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5098..5967
                     /locus_tag="WM704_001851"
     CDS             5098..5967
                     /locus_tag="WM704_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFA9341207.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
BASE COUNT         1368 a         1609 c         1858 g         1436 t
ORIGIN      
        1 tgtgtgaatg atgaaataaa aatgccgtct gaacccgatt ggattcagac ggcattttgt
       61 atggcaggat ttattcgctt tccaactgac cgacccattc tgacaaggca gtcaggcgtt
      121 gttcggattt cgccacgaac gggttttcgg tatcccacgc gtagcctgcc aaaatcgaag
      181 aaagcatacg gctgatttcc gggctgcggt cgggcgcgta cacgacgttt tggaagcgga
      241 aatcatacca gccgtccaca taggtgcgga acgcgtctac gccgatcatc aggggttcgg
      301 caaattcggt ttgccaatcg gcggcttcgc ttttgagttg ttttgtcagc agatcggcag
      361 caagttcggc ggagtgcagc gcgatggtta cgcccgacga gaacacgggg tcgaggaact
      421 cggcggcatt gcccaacagc gcgaaatgcc tgccgtgcag tgatttgacg ttggcggaat
      481 agccttggat ggagcggaac ggaaaatcgt tttcccaaac ggctttgtcc aaaatttcgc
      541 ttaacatcgg gcattcgtaa acaaattttt tcaacaccgt ttccgattcg ccggcaagtt
      601 tgtcgggtgt gccgaccacg ccgacggaac agcggttgtc gccgaaggga atcagccaaa
      661 tccacacgtc gcggttgttg cggatgggtg gtaatcagga ttttgttgcg gtcgaatttc
      721 gggtgggtaa tgttgtcgtc gatgtgcgtg aaatgcgttt ggcgcggcgg caggtgcgag
      781 ggcgtttcca agtttagcag gcgcggcagc acgcgtccgt agccgcttgc gtccaagacg
      841 aatttcgcgg tcagttcata gctctcgccg gtgtcggttt cgatgttcaa gcgggcaaaa
      901 tcgccgctgt tgtcgaacgc ggttacgccg tgcccgaaac gtacttcaac gccttgtttg
      961 gcggcttctt caatcaggat tttgtcgaac acggcgcggc ggacttggta aaccgtgccg
     1021 gggccgtctg aaaatttatc ggtgaaatca aactcggtat agcggctgcc ccacgaaaac
     1081 gccgcaccgt ttttcaactg aaagccgggc ccggtgcgaa cggcatcggc aaaaccggct
     1141 tcttccagca tttccataca gtgcggcagc aggctttcgc cgatgacgaa gcgcggaaag
     1201 tgctgttttc caacacgcag acttgataac cttttttgcg gagcagggcg gatgcggccg
     1261 aacctgccgg acctgcgccg attacggcga catcgaattg ggtggacatt gggaaatcct
     1321 gatggtttga gtggaaaagg aaatgatatt gtacaggcag caaatgccgt ctgaaactgt
     1381 ttgcggaaga aatttatccg gtgcgggcgg ttgctcccaa tgcgtcagtc cggtttgaaa
     1441 aatgccgtct gaaacgggaa atgttcagac ggcatttgat tttcagggtt attttacgcc
     1501 gtaacgcggt tggagcaggg cgcacgtccg gtgtccccgg cggcttcggc ggctgcgccc
     1561 ggcaattttt ccgcaggccg cgcccgcatc gtttgccgtg gtttccggcc gggtattttt
     1621 ttcgcgctga gccctggcgg attcgtagcg gttgcgttgt ctcggttcgc ttgtttcggt
     1681 tttttccggt tttgagcctt cctgttccca ccatcggggc tcgaagcctt cgatgcgttc
     1741 gatgagcagc ttgttgccgg tcagttcttt gatggcctca aacattttct gttcggattc
     1801 gtccatcagg gaaatcgcca cgccgtccgc accggcgcgc cccgtgcgtc cgatgcggtg
     1861 gatgtagtct tcgggctggg cgggcatttc gtaattgatg acgaagggca gttcggcgat
     1921 gtccagccca cgcgcggcga tatcggtggc gacgaggacg cgcaggctgc cgtctttgaa
     1981 ggcgttgagt gtttcgagcc tgctttgttg ggaacggtcg ccgtgtatcg cctgtgcgga
     2041 caggttgcgg cgcaccagtt cgcgcgttac gcggtcgacg ctttgtttgg ttttgcaaaa
     2101 gacgataact tggttcatat gcagatcgac aatcagccgt tcgagcaggt tgcgcttttg
     2161 gaaggtatcg acggcgatga tgtgttgttc gacgttggcg ttggtggtgt tttgggcggc
     2221 aacctcgacg gtttcgggcg cgttcatgaa gtcttgcgcc agtttgcgta tcggggcgga
     2281 gaaggtggcg gaaaagagca gggtttggcg ttggcggggc agcatctgca tgattttgcg
     2341 gatgtcgtcg ataaacccca tatccaacat gcggtctgct tcgtccagaa cgacgatttc
     2401 ggctttgttt aaactgatgt ttttctgttt cacatggtcg agcagccgtc cgacggtggc
     2461 gacgactatt tcgcagccgg cacgcaggtc ggcggtttgt ttgtccatgt tgacaccgcc
     2521 gaagaggacg gtatgccgca gcggcaggtt tttaatgtag ccctgcacgt tttggtcgat
     2581 ttggtcggca agttcgcgcg tcggggtcaa cacgagcata cgcacggggt gcattgcagg
     2641 cgaggtgctg gcggtggcgt aacgtttgag gcgttccaga ctgggcagca taaaggcagc
     2701 ggttttgcct gtgccggttt gcgcggcggc aagcaggtcg tgtccggcca gtgctttggg
     2761 aatggccgcg gcttggatgg gcgtcgggtt ttcgtaacct tgcgcggtca gtgcggaaac
     2821 cagttccgta cccaaaccta aagaggaaaa tggattactc atgattgtag tctttctttc
     2881 agaccttatg ccgtctgaag cgggaaaccg ataggacggg gaaataaact gcccgcctgt
     2941 gaacggcggg gacgggaatt tgtgtgcgga ttatgccata aaacggtgcc cgccccaatc
     3001 gcgggcgcgt ccgcagattg gaaatcctgc ttaaaaaatg tacaatggcg cacttttttg
     3061 aaacgcgggc ccattatgca catcggcggt tattttatcg acaaccccat cgcacttgcg
     3121 ccgatggcgg gcattaccga caaacccttc cgccgcctct gtcgggcgtt tggcgcaggt
     3181 tgggcggtgt gcgaaatgct ggccagcgat ccgacgctca ggaataccgg aaaaaccctg
     3241 caccgcagtg attttgccga tgaaggcggc atcgttgccg tgcagattgc cggcagcgac
     3301 cccgaacaga tggcggatgc ggcgcgttac aacgtcggac tcggggcgca ggtcatcgac
     3361 atcaatatgg gctgccccgc caagaaagtg tgcaacgtcc aagccggtag cgcgctgatg
     3421 caggacgagc cgctggttgc cgccattttg gaggcggtgg tcaaggcggc gggcgtaccc
     3481 gttaccctca aaacccgttt gggttggcac gacgaccatc aaaacctgcc cgccatcgcc
     3541 aaaatcgccg aagattgcgg cattgccgcc cttgccgtcc acgggcgcgc gcgcacgcaa
     3601 atgtacaaag gcgaggcgcg ttacgaactc atcgccgaga ccaaaagccg tctgaacatc
     3661 ccggtctggg tcaacggcga catcacttcg ccgcaaaaag ccgccgccgt cctcaaacaa
     3721 accgccgccg acggcatcat gatagggcgc ggcgcgcaag gcaggccgtg gtttttccgc
     3781 gatttgaagc attatgccga acacggcgtt ttaccgcctg ccttgagttt ggcagaatgc
     3841 agagccgcca ttttgaacca catccgcgcc atgcacgcgt tttatggtga gaccgtcggt
     3901 gtgcgcatcg cacgcaaaca cataggctgg tacatcggcg aaatgcccga cggcgaacag
     3961 gcgcggcgtg aaatcaaccg cttggacaat gcggcggcgc aatacgacac acttgccggt
     4021 tatcttgaaa ggcttgccgg aaaaaccgac cgttgggcgt gcggctatcg ggaagggtag
     4081 ggcagtattg ccatgccgcc gttcgggttc ggacggcatc tgtctgcatg gttcggaggt
     4141 cgggcggaat cccggccggc gaatgtcgaa aacaataaat gccgtctgaa aacgagtgga
     4201 acgggtttcg ccaaaaggct ttcagacggc attccgtctt aaaaacattt caatcaaaag
     4261 gatatgcgat gaaccctgcg actgcggaca ttgcgcaatg tatcgagcag aacttgaacc
     4321 aatatttcaa agacctgaac ggcaccgaac cttgcggcgt gtacgatatg gtactgcatc
     4381 aggtggaaaa gccgctgctg gtgtgcgtga tggagcaatg cggcgacaac cagtccaaag
     4441 cctccgtgat gctgggactg aaccgcaata ccttgcgtaa gaaactgatt caacacggtt
     4501 tgctgtgaat atgtcggcaa ccgtccgtat cttgggcatc gacccgggca gccgcgtaac
     4561 gggtttcggc atcatcgatg tcagggggcg cgatcatttt tacgtcgcct ccggctgcat
     4621 caaaacgcct gccgatgagc ctctggcaga caggattgcc gtgattgtgc ggcatatcgg
     4681 cgaagtcgtt gccgtttaca agccgcaaca ggcggcggtg gaacaggtgt tcgtcaacgt
     4741 caatccggca tcgacgctga tgctcggtca ggcgcgcgga gcggcattgg cggctttggt
     4801 cagccataag ctgcccgttt cggaatacac ggccttgcag gtcaaacagg cggtggtcgg
     4861 caaaggcaag gcggcgaaag aacaggtgca gcatatggtg gtgcaaatgc tgggactttc
     4921 gggaacgccg caggcggatg cggcggacgg tcttgccgtc gcgctgaccc acgccttacg
     4981 caaccacggg cttgccgcca aactcaatcc ttcggggatg caggtcaagc gcggaaggtt
     5041 tcaatagttt cagacggcat ttgtattttg ccgcctgaaa agaaaatgtg taccgagatg
     5101 aaatttatat tttttgtact gtatgttttg cagtttctgc cgtttgcgct gctgcacaag
     5161 attgccggcc tgatcggttc gcttgcctac cttctggtca aaccgcgccg ccgtatcggc
     5221 gaaatcaatt tggcaaaatg ttttcccgaa tgggacgaag aaaagcgtaa aaccgtgttg
     5281 aaacagcatt tcaaacacat ggcaaaactg atgctcgaat acggcttata ttggtacgcg
     5341 cctgccaaat gcctgaaatc gctggtgcgc taccgcaata agcattattt ggacgacgcg
     5401 ctggcggcgg gggaaaaagt catcatcctg tacccgcact ttaccgcgtt cgagatggcg
     5461 gtgtacgcgc ttaatcagga tgtcccgctg atcagtatgt attcccacca aaaaaacaag
     5521 atattggacg aacagatttt gaaaggccgc aaccgctatc acaacgtctt ccttatcggg
     5581 cgcaccgaag ggctgcgcgc cctcgtcaaa cagttccgca aaagcagtgc gccgttcctg
     5641 tatctgcccg atcaggattt cggacgcaac aattcggttt ttgtggattt tttcggcatt
     5701 cagacggcaa cgattaccgg cttgagccgc attgccgcgc ttgcaaatgc aaaagtgata
     5761 cccgccattc ccgtccgcga ggcggacaat acggttacat tgcaattcta tcccgcttgg
     5821 aaatcctttc cgagtgaaga cgcgcaagcc gacgcgcaac gtatgaaccg ctttatcgaa
     5881 gaacgcgtgc gcgaacatcc ggaacaatat ttctggctgc acaagcgttt caaaacccgt
     5941 ccggaaggca gccccgattt ttactgacta cataaaatta caaaacaaat caggcgtttc
     6001 agatcaaaaa ccccgattgt ttttgggaat ttgaaacccg ggttgtacaa acgggatttg
     6061 ccggacggtt ttaacggttc agttgtttgt aaaaacaatg cttttttaaa attgacaaaa
     6121 aacgaaatcg gttttaaagg cttattccga gaacaaaggg gagtggatgc cgaaaacccg
     6181 gttaatatat tatagtggat taacaaaaac caatacggcg ttgcttcgcc ttagctcaaa
     6241 gagaacgatt ccctaaggtg ctgaagcacc a
//
LOCUS       DAWTHD010000111         6237 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWTHD010000111 DAWTHD010000000
VERSION     DAWTHD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.111"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..650)
                     /locus_tag="WM704_001852"
     CDS             complement(<1..650)
                     /locus_tag="WM704_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFA9341208.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLS"
     gene            709..1710
                     /locus_tag="WM704_001853"
     CDS             709..1710
                     /locus_tag="WM704_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9341209.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSPEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            1804..2652
                     /locus_tag="WM704_001854"
     CDS             1804..2652
                     /locus_tag="WM704_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFA9341210.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            2679..3161
                     /locus_tag="WM704_001855"
     CDS             2679..3161
                     /locus_tag="WM704_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFA9341211.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(3245..4813)
                     /gene="msrAB"
                     /locus_tag="WM704_001856"
     CDS             complement(3245..4813)
                     /gene="msrAB"
                     /locus_tag="WM704_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFA9341212.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            4959..6212
                     /gene="ftsY"
                     /locus_tag="WM704_001857"
     CDS             4959..6212
                     /gene="ftsY"
                     /locus_tag="WM704_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFA9341213.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRTESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRGRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         1357 a         1629 c         1883 g         1368 t
ORIGIN      
        1 tctgacaaag tttctgcata ccgtgccagt tgcgccatat cgcttacgga ggcatcgata
       61 aagggcggcg tgtgggattt tgcaccgaac cggacggttt tcatgcccaa tgcttttgcc
      121 tgatggaggt tgtccgcgct gtcgtccacc ataatgcagc attcgggcgg tacgtccaac
      181 aggcggcaga cattgagata cgcttgcgga ttgggtttgt acagcagtcc gaaatcatcc
      241 gtgccgaaca gtgcgtcgaa acagttttcc aaaccgagtg cgccggcaac ggcacggacg
      301 taaaacgacg ggccgttgga aaaaaccgcc ttgcgcccgt tcagacggca tagggtgttt
      361 tccgtatccg ccatgccgtg cagcctggtc aggattgcat cgatcggatg gctttcgcgt
      421 aaaaattccg caatgtcgat ttcgggatgg tggatttgca gtccggcgag cgttgcgccg
      481 taacggtgcc aatagtcttg acgcaggtcg gacgcggcgg attcggagag tttgaggcgg
      541 cgtgccatat agcgcgtcat agcgcggttg atgaggtgga agatgcctgc gtcggcatcg
      601 tgcagcgtgt tgtcgaggtc gaacagccac acggtcgggt tttcttgcat tttgaaccgt
      661 gaaaatttgt tagaatgtta ttttacagcg aataaaggaa gactcggaat gaaacggaaa
      721 atttggctgc tgccgctgct ggcggtttcg gcatacctgc aggcgcagac ggaagtcagg
      781 ctggcggtgc ataagtcgtt cagcctgccc aaagggctga ttgcgcgctt cgagcgggca
      841 aacgatgcga aagtgtcgat tattcaggcg ggcggcgcga acgaaatgct caacaaactg
      901 attttgagcc gagccaaccc gattgccgac gcggtgtacg gtttggacaa cgccaatatc
      961 ggcaaggcgc gggaaatggg cattttggcg gcggcgcaac ccgaatccgc ccccgtcgcg
     1021 gtcgggctgc cttcggcttt ggcggtcgat tacggctatg tgtccgtcaa ttacgacaaa
     1081 aaatggtttg aaggcaaaaa gctgcccctg ccgcaaaccc tgcaggattt gacccgcccc
     1141 gaatataaaa acctattggt cgtgccgtcc cccgccacat cttcccccgg cttgggcttc
     1201 ctgatggcga acatcggcgg tctgggcgaa gaaggcgcgt ttaaatggtg ggcacagatg
     1261 cggcagaacg gcgtgaaggt cgccaaaggc tggagcgagg cgtattacac cgacttttcg
     1321 cacaacggcg gcgcgtatcc gctggtggtc ggttatgccg ccagccctgc ggcggaagta
     1381 tatttttcca aaggcaaata cagcgagccg ccgacgggca acctgttttt aaaaggcggc
     1441 gtattccgtc aggtcgaagg cgcggcggtc ttgaagggcg cgaaacagcc gaagctggcg
     1501 gcaaaactgg tgcaatggct gcaaagtccg gaagtgcagc aggcggttcc gtctgaaatg
     1561 tgggtttacc ccgccgtcaa aaacacgcgc ctgcccgacg tgttccgctt cgcccaagcc
     1621 ccggcgcaca ctaccgcccc cgcgcagcgc gatattgatg cgaaccagcg cggatgggtt
     1681 tcccgttgga tcaggacggt tttgaaataa aacaaacata ccgccccgca gggcttcaga
     1741 cggcattttt acacctgtgc cgattacgcc gcacggggcg gatgttcgat cgagaggaaa
     1801 acaatggact tcaaacaatt tgatttttta cacctgatca gtgtttccgg ttgggggcat
     1861 ctggctgaaa aggcgtgggc gttcgggctg aaccttgccg ccgcgctgct tattttcttg
     1921 gtcgggaaat gggcggcgaa acgcattgtc gccgtgatga gggcggcgat gacgcgcgcg
     1981 caggtcgatg ccacgctgat tagttttttg tgtaatgttg ccaatatcgg cttattgatt
     2041 ttggtgatta ttgccgcatt gggcagattg ggcgtttcca caacatccgt aaccgcctta
     2101 atcggcggcg cgggtttggc ggtggcgttg tccttaaaag accagctgtc caattttgcc
     2161 gccggcgcgc tgattatcct gttccgcccg ttcaaagtcg gcgactttat ccgtgtcggc
     2221 ggttttgaag gatatgtccg ggaaatcaaa atggtgcaga cttctttgcg gacgaccgac
     2281 aacgaagaag tcgtgctgcc caacagcgtg gtgatgggca acagcatcgt caaccgttcc
     2341 agcctgccgc tttgccgcgc ccaagtgata gtcggcgtcg attacaactg cgatttgaaa
     2401 gtggcgaaag aggcggtgtt gaaagccgcc gccgaacacc ccttgagcgt tcaaaacgaa
     2461 gagcggcagc ccgccgccta catcaccgcc ttgggcgaca atgccatcga aatcacatta
     2521 tgggcttggg caaacgaagc agaccgctgg acgctgcaat gcgacttgaa cgaacaagtg
     2581 gtcgaaaacc tccgcaaagt caatatcaac atcccgttcc cgcaacgcga catacacatc
     2641 atcaattctt aaacgccgtc tgaaaggggg ggtgggaaat ggacgcgttg cacaccatcg
     2701 cccgaaacct gacgaaaaaa cgtcaaaccg taagctgtgc cgaatcctgc acgggcggaa
     2761 tgcttgccgc cgcattcaca agcgttgcag gcagttcgca atggttcgac cagagttttg
     2821 taacatacag caacaaagcc aaagaatacc gcttgggcgt gttgcccgaa accctgctcg
     2881 aacacggcgc ggtcagccgc caaaccgtct atgagatggc gcgcggcgcg aaagccgtgg
     2941 ctcaggcgga ttacgccgtc ggtatttccg gcatcgccgg cccgggcggc ggcgaaagca
     3001 aacccgtcgg cacggtttgg ttcgggtttg cctttccggg cggaagttgc gaagcaatgc
     3061 gccgttttga cggcaaccgc gaatccgtcc gcgcgcaggc ggtcgccttc gcgttggaac
     3121 ggttggccgg gctggttgaa aacggcggcg atgctgtctg aacaaatccc cgccggataa
     3181 aaaatgccgt ctgaaacgtt gcaggtttca gacggcattt tgccgggtta ggcggcggtg
     3241 cggcttattt cactttgccc ttcaacgcgc cgtagcctgc cgcgtccatt tgttccagcg
     3301 ggatgaattt caagctcgcg ccgttgatgc agtagcgcag tccgcctttg tcgcgggggc
     3361 cgtcggggaa gacgtgtccc aagtgcgaat cggcggcgcg gcttctgact tcggtgcggc
     3421 gcatattgaa gctgaaatca tcgtgttcgg taacggattt tgcatcaatc gggcgcgtga
     3481 agctcggcca gccgcagccg gaatcatatt tgtcggcgga gctgaacagg ggttcgccgc
     3541 tgacaacgtc cacataaatg ccgggtttga acaaatggtc gtattcgtgg ctgaaggcgt
     3601 attcggtcgc gctgttttgg gtcacttggt attgctcttc ggtcagggtg cgtttgagtt
     3661 cggcgtcact cggtttttta tacgttgccg cgtcgaagcc tttgccttgc ggtgcggctt
     3721 tggttttgcc cggcagcggt tcgtcggctt tgcggatgtc gatgtggcag tagccgttgg
     3781 ggtttttaat caggtagtcc tgatggtatt cctcggcgtc gtagaagttt ttcagcggct
     3841 cgttttcaac aacgaggggc agttggtatt tttgctgctc gcgtttgagg gcggcggcga
     3901 tgacggcttt ttcggcgggg tcggtgtagt acacgccgct gcggtattgc gtgccggtgt
     3961 cgttaccctg tttgttgagg ctggtcggat caacgacgcg gaaataatat tgcaggatgt
     4021 cgtccaggct gagtttgtcg gcatcgtagg tcactttgac ggtctcggca tggcccgtat
     4081 ggcggtagga tacgtcttcg tagctcgggt tttccgtgtt gccgttggcg tagccggata
     4141 ccgcgtcaac cacgccgtcg atgcgttgga aataggcttc caagccccag aagcagccgc
     4201 cggcgaggta gatggtgcgc gtgttcatga ttgctgaatc ctttttctga gtgtcgggtt
     4261 tgtagaacga atgtttcaaa ctgcccaaat cggcattcgg gttgcggatt aacgccaatg
     4321 cctgcgcttc gttgatgctg cctttgacga tgcgctgcac gtcgccgtct ttaccgatta
     4381 acgcccaaga aggataaacg ctgatattca ggttttgggc gatcgtgccg ccgttgtcgg
     4441 taacgacggg cagcttgggg tagttcaaac cggcatacca tttttgaaac tcgccgtctt
     4501 ttttctcgtg caaaaagccg ggggaggcga cggtaatcag gttggcggag ctgaattttg
     4561 catcttgcgc ccatttctcg gcctgtccca attcggacaa acataaagga caccagctcg
     4621 cccaaaattt aatcagcgtc ggtttgtctt ttttcaaata aacactggcg gggcggttgt
     4681 ccgcggtttt taacgtggat aaagtgtgcg gcacggtcgc ggccccggca tcgacgattt
     4741 tgggcgaaca agcgcccagc gcaagcaggc agccgaactt ggcgcaaagg gaaaagaaag
     4801 tacggtgttt cattttgatg tttcctgtgt ggacggtttg catgattaga cgtttgagat
     4861 gccgaaacct tacggcccgg attttcagac aaccttgccg cgcaaaatac gctacaatac
     4921 gccctatttc aagtttctaa aattaaaagg aaaattcaat gttcagcttc ttccgtcgca
     4981 agaaaaaaca ggaaacgccg gctctcgagg aggcccaagt tcaggaaacc gcagcaaaag
     5041 tagaatctga agttgctcaa atagttggaa atattaaaga agatgtcgaa tctttagcag
     5101 aaagcgtcaa agggcggacc gaatctgccg ttgaaaccgt cagcggtgcg gttgaacagg
     5161 taaaggaaac cgttgccgag atgccgtctg aagcagggga agcggcggaa cgcgttgaat
     5221 ccgcaaaaga agctgttgcc gaaaccgtcg gcgaggctgt cgggcaagtt caagaagccg
     5281 ttgcgacaac tgaagaacac aagctcggtt gggctgcgcg tttgaaacaa ggcttggcca
     5341 aatcacgcga caaaatggcg aaatcgctgg ccggcgtgtt cggcggcgga caaatcggcg
     5401 aggatttgta cgaagagctg gaaaccgtgc tgattaccgg cgatatgggc atggaggcca
     5461 ccgaatacct gatgaaagac gtgcgcggcc gcgtcagcct caaagggctg aaagacggca
     5521 acgaattgcg cggcgcgttg aaagaagcct tgtacgacct gattaagccg ttggaaaaac
     5581 cgctggtctt gcccgaaact aaagagcctt tcgtgattat gcttgccggt atcaacggcg
     5641 cgggcaaaac cacgtctatc ggcaaactcg ccaaatattt ccaagcgcag ggcaaatccg
     5701 tattgctggc ggcgggcgat accttccgcg ccgccgcccg tgagcagctt caggcttggg
     5761 gcgggcgcaa caatgtaacc gtcatttcac aaaccacggg cgattccgcc gccgtgtgct
     5821 tcgatgccgt ccaagccgcc aaagcgcgcg ggatcgacat cgtgcttgcc gacaccgccg
     5881 gccgcctgcc cacgcagctt catttgatgg aagaaatcaa aaaagtgaag cgcgtgctgc
     5941 aaaaagccat tcccggcgcg ccgcacgaaa ttatcgtcgt actcgatgcc aatatcgggc
     6001 aaaacgccgt caaccaagtc aaagcctttg acgacgcatt ggggctgacg gggcttatcg
     6061 ttaccaaact cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgct tccgaccgcc
     6121 ccgtccccgt ccgctacatc ggcgtgggcg aaggcataga cgacctgcgc ccgtttgacg
     6181 cgcgcgcgtt tgtggacgca ctgctggatt gagccgaaat gccgtccgaa aacggca
//
LOCUS       DAWTHD010000112         6140 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWTHD010000112 DAWTHD010000000
VERSION     DAWTHD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.112"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            261..1757
                     /gene="ccoG"
                     /locus_tag="WM704_001858"
     CDS             261..1757
                     /gene="ccoG"
                     /locus_tag="WM704_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFA9341214.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
     gene            1760..2308
                     /locus_tag="WM704_001859"
     CDS             1760..2308
                     /locus_tag="WM704_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFA9341215.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(2650..3825)
                     /locus_tag="WM704_001860"
     CDS             complement(2650..3825)
                     /locus_tag="WM704_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HFA9341216.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(3983..4099)
                     /locus_tag="WM704_001861"
                     /pseudo
     CDS             complement(3983..4099)
                     /locus_tag="WM704_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4262..4336)
                     /locus_tag="WM704_001862"
     tRNA            complement(4262..4336)
                     /locus_tag="WM704_001862"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4301..4303),aa:Glu,seq:ttc)
     gene            complement(4354..4430)
                     /locus_tag="WM704_001863"
     tRNA            complement(4354..4430)
                     /locus_tag="WM704_001863"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4394..4396),aa:Arg,seq:acg)
     gene            4548..5933
                     /locus_tag="WM704_001864"
                     /pseudo
     CDS             4548..5933
                     /locus_tag="WM704_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
BASE COUNT         1489 a         1407 c         1659 g         1585 t
ORIGIN      
        1 ctttgttttt taagagtatt ttaattttgt tcgggcggtg cggtaaaatc agggcgtttt
       61 gatgcggatg ggaaatgtat tcgcccaata catccggacg gcacataaag ttgtacaata
      121 gcgggaatat attttgggaa aagcggattt tctcaaaact tgaaacacaa tgccttaaaa
      181 aaacaagaaa aatgacgtct gaaaagcaaa acggcggcgg taacgggcaa accatcaatc
      241 aaaaatctaa ggaatgcaga atgaccacgg aaaaccaagc cggcagtccg gcatccggaa
      301 tcggcacgtc cgaacaaaca aaagccgcgc cgaaagtgaa aaaaacgttc gatccgcgtg
      361 ccagcgtgat tcaaatccat cccgaaggcg aacgcatcca tcccaaaaag gcggaaggac
      421 ggttcgccaa actgcgtatc gccgccgtat tggcgaccca gtttgtgttt tacgtcattc
      481 cgtggttcaa ttggagcgga aggcaggccg tcgttttcaa tatccccgaa cggcatttct
      541 tcattttcgg attgtcgttg ggggtgggcg atttgattta ccttgccctg ctgctgatga
      601 tttgcgcctt cgggctgttt tggtggacga cgattgccgg ccgtttgtgg tgcggctatt
      661 cctgcccgca aacggtttac accgaaatta tgctgtggat cgacaacctg gtcgaaggcg
      721 atagaaacaa acgcctgaag ctggaaaaat cgccgtggaa tttcaccaaa atccgcatca
      781 aagccaccaa atacctgttg attttccttg tctgcgcgtg gacgggcatc acgtttgccg
      841 gctggtttgt ccccatccgc cagtttgttc ccgatttatt caccggagca gcaggcggcg
      901 gcgcgatgtt tgccgcagcg ttttacggct ttatgacctt cttcttcgcc catattatgc
      961 gcgaaaaagt gtgcctgcat atgtgtccgt acgcacgttt ccaaagcgcg atgttcgata
     1021 aggacacgct gattgtttct tatgacgcgg agcgcggcga accgcgcggc gcccgcaaga
     1081 aaacggtcaa caaggaagag gcgggtttgg gcgactgcat caactgtgcg atgtgcgtcc
     1141 aagtctgccc cgtcggcatc gacatccgca acggcctgca ataccaatgt atcggctgcg
     1201 ccgcctgtat cgacgcgtgc gatgagatta tggacaaaat gggttatccg agcggtttga
     1261 tacgctatac gaccgaaagc gcgctggaac acgaatatgc cgaaaaagac attaaaaaac
     1321 ggctgctcag accgcgcgtg gcaggttacg gcgcggtgtt ggcagtggtt gtcgccgcct
     1381 tcctggtcgg tttgtccacg cgcaaaatgg tcgaagtcga tattctgaaa gaccgtggcg
     1441 taatggtgcg cgaaaacgcc aaaggttggc tggaaaacgc atatagcctg cgtatcatca
     1501 acaacagcga aaaagaacag ctgattaccg caagtgtcaa aggatttgac gaaatcgccc
     1561 tgaccgggct gcctgaaggc ggtataaaag tggctccgcg cgaaacaata acccttcccg
     1621 tccaagtgtc caccattccg gaatacgcgg acaaaggcag tcatcctatc gagtttatct
     1681 tccaataccg ggaaagcggt gcatcagacg gcaagccggt tgttttggaa gaagacgcaa
     1741 cctttatcgg agaataaccg tgtctcaaaa caatccaatc aagccttggt acaaacacgt
     1801 ctggccgtgg gtcttgatgg cggggccgat ttttgtcgtc atcgccagcg tcgctatgtt
     1861 ttttgtcgcg cagcagcacg cgacagattt ggttacggac gattattata aggatggcaa
     1921 gcatatcgac atccagcttc atcgggatga agaagccgtc agacggcata tcggggtgca
     1981 ggtcctcatt tctcccgata tgaatgcggc aaaagtgttt gtcggcggcg agtttgacgg
     2041 caaacagcct ttgaacctgc tgctgatgca cccgacccgc aaggcggacg atcaaaccgt
     2101 cgccctcaag cccgtcggca gcgcgcagaa cggcagggcg gaatatgagg cggtgttcaa
     2161 aacccttccg ccggccaacc actggtatgt gcgcgtggag gacgcggcag gcgtgtggcg
     2221 cgtcgagaac aaatggatta ccagccaggg caatgcggtc gatttgaccc cgatggacaa
     2281 acttttcaat aatgcaggaa gcaaataaaa aagccgtgtt ttcattgcat tggtttgtta
     2341 tatgcaatgt cgggcaggca ttgtttgatt attcggcgtg atggtctgat ttgtcggacg
     2401 aaaataaatg tatctcatcc cgtttatttt ttaacatcat tccgaaaggc agcctgaaaa
     2461 atgccgtctg aaaggcttca gacggcattt gtgttgtatg tccgacataa tgcggaaggc
     2521 attttgttca cataaaaacg tttttatttc tctccgtttt ttcgacactg ttccggaaca
     2581 caacctgaaa aacgccgtct gaaagccctt cggacggcat tttgtttgca aatcaaatcc
     2641 tacctgatgt cagcgtccga gcgttgcaac aggttcgctc cccgtcagca gatattgtat
     2701 cgtttcgcgg acggtgcgga tggcattacc gaagggaagc tggtcttttc ccctgaaaaa
     2761 caacccctta tcaacttctc cacggaatgc ggcagcaagc tggatatcaa tacagaattg
     2821 ccctgctttg gaaagcccgt cgcgcagacc gcagctggtc aggcagttta aaccttgggt
     2881 acagcggcgc gggtcggctt tggcgtttgc ctgaagtttg ccttcgcgcc tgatatagct
     2941 gtctaggaat ttggtgcgga taccgcgcgc cggcaaaccg gcaacagaca taaattcgac
     3001 tactttttca gtttccgcac tggcgagcgt ttttttaaag ttgaggtgtg catctccttc
     3061 ttcggtaacg gcaaaagccg taccgatttg aacggcggag gctccccagt tctttagggc
     3121 ggttttgact ttttcaaaat ttgccatgcc tcccgcaaga ataagcggga ttttttctcc
     3181 ctccagcccc aaatttttaa acacttcaaa cgtttcctca atcacgcgtt tgaagtcgaa
     3241 tttggcatcg tttacgcctt caacggttga tgcacccaaa tgtccggccg catgggcagg
     3301 atgttcgact acaatcgcat cgggcaatat gccttttttc atccaacgtt tcaagacgat
     3361 attaataccg cgcgattcgg acagaatcgg cagcagcgcg acatccttat gatagccctc
     3421 ggtcatttcc ggcaggtcta aaggcaggcc ggcacccatc acgaccgcat ccgcccctga
     3481 ttcgcaagcc tggcggacat acgcggcgtg gtctttgacc gccttcatca cgttgaccgc
     3541 aatcagtcct tttccctctg aagcactttt ggctttttgg atttccctgt ctaatgcggt
     3601 acagttcaaa gacgtatatt tctcttcact cggattgatt tgggattcgg cgagcaggtc
     3661 ttcgtgaagg tggcgcaaat ctacactggc aatcgttccg atgccgtttt cacgcgccac
     3721 cgcgctggat aaacccgatg cggaaacacc gacccccata ccgccttgca cgatgggaat
     3781 aagggatttt ccacgaataa tcaaagggtc aaaaatattc tgcatcagtt tctccgaata
     3841 tacgaatcag tcaggctcat gaaaaatggt tttaattgtt ttagaaccat tactcaaatt
     3901 tggtattata ctctaaatat tgcagtggta aacagaaaag cagaaggagc tttggaatat
     3961 gctgttatat ctgtaccaat cggacaaaat atagtggatt aaatttcaac cggtacggcg
     4021 ttgcctcgcc ttgccgtact atttgtactg tctggggctt cgtcgcattg tcctgattta
     4081 aatttaatcc actatattga acggacaccg catccggtta tgggatatcg gtttgcatgg
     4141 caaaagtttg aatatgattc gattacgtct gagattctga tgaagtgggt aggtaaagat
     4201 atggtaacga attgccgagg caataaaaat ccccgaggga ttatttcggg gatttggaat
     4261 ctggcacgcc cacggggaat cgaaccccgg ttaccgccgt gaaagggcaa tgtcctaacc
     4321 gctagacgat gggcgcgtat tgtattttct tactggcgca cccggagcga ttcgaacgcc
     4381 cgaccctctg gttcgtagcc agatactcta tccaactgag ctacgggtgc gctgcaataa
     4441 atttttgaat tttatggtaa tcatcggatt ctgtcaatta ttttgttgga tgaaatgcct
     4501 taaaagtgta aaatatcggt ttgttgtttg aatttaaaga gaataaaatg attgttgctt
     4561 ttcgctggcc cttgctggaa aaggcgtttt tacggtttgg aatgcctgtt gccgtggttg
     4621 atttggaatc gacgggcggc aatctgtatg aagacagggt aaccgaagtg gctttggtca
     4681 agtttgggca gggaagggcg gagaggtatg aatggctggt caatcctcaa aaaccgattc
     4741 cgaagtttgt ggcggaactg acggggattt cagacggcat ggttgccgac gcgccggtgt
     4801 ttgctgaaat tgccggcggg ttgttttcgg tattgaaggg gtgcgtgctg gtggcgcaca
     4861 acagccgttt cgactatacg tttttaaagc atgagtttca tcgtgcgggt atcggatttt
     4921 catcgcctgc tttgtgcagt gtgcagcttt cgcgtcggct gtatccgcag ttttacaagc
     4981 acagcctgga cggtatcatc gaaaggttgg ggattgttgt ggaagacagg catcgtgcga
     5041 tggcggatgt atcggcgttg tgtgattatt tggaatacag cctgtcggca cacgggcttg
     5101 aggagtggag caggcagtgt ttccgtttga cgaatccgaa acttcttcct gccgcgcttc
     5161 ccgaacggtt gagggaacgg ctgtacggtt tgcccgacgg tacaggtgtg cttgcctgtt
     5221 tcgacggcgg gggaaaagta aattatatcg gtacgtttga acgggcatat ggcgaggttg
     5281 cggctttgtt ggattccgga aaagcccccg tttgattggt gcaatacgga ggaagtccgt
     5341 ttttttcccg cattgggcag cctgcatgca tataagatta aagcggaatt ggtcggacgt
     5401 tatcattcgg gttgttatgt atctgccaaa aatctgctta aaacatttac gaccgtcagg
     5461 tttgaaaaag gttcagacgg catgttgaat gcgaaaacag cggctttgaa aaacggtgtg
     5521 acggataatc cgcctaccgg attatttgcc aataaaaagg cggcgagacg ggctttgtcg
     5581 tcgtgggcgg aaacatacgg attgtgtcct gcctcagccg gtatccttcc ggacggttat
     5641 gcagaggatg agccttgtcc cgtatatgtt tcaggccgat gcgataaggc gtgcggccgt
     5701 tttgacgaac aagttttggc gtttgcacac aagttgcctg ttttggattg gggaaaaatg
     5761 catgaggtgg aaattaccga aaccgatcct ttaacggggg aaaagtcgtt ctgcatggaa
     5821 tgggcggcgc attggaaatg gatgacgggc tttggtattt cgataaagat ttaccggatg
     5881 tgtttaaggc gaagtttaaa acggacagga aaaatattaa ggaaatcggt taaatcgata
     5941 ccggttggtg aaagctggta agtatgtaaa cagccgttcg tattgattgc ctgatcggtt
     6001 tggattggaa tgtgttgccg tttgcaggta atgtgtctga agatgccgtc tgaatttttt
     6061 cagacggcat ttttgccgac gcggtttgcg tgccgtgtcg gtttgcccgt ccgtcccgag
     6121 gcatcggaca agcagggtgg
//
LOCUS       DAWTHD010000113         6139 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWTHD010000113 DAWTHD010000000
VERSION     DAWTHD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.113"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            44..433
                     /locus_tag="WM704_001865"
     CDS             44..433
                     /locus_tag="WM704_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9341217.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            complement(506..1756)
                     /locus_tag="WM704_001866"
     CDS             complement(506..1756)
                     /locus_tag="WM704_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFA9341218.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(1823..2065)
                     /locus_tag="WM704_001867"
     CDS             complement(1823..2065)
                     /locus_tag="WM704_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341219.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            complement(2010..2174)
                     /locus_tag="WM704_001868"
     CDS             complement(2010..2174)
                     /locus_tag="WM704_001868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341220.1"
                     /translation="MSNQTKIQAIKQASEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            complement(2238..4006)
                     /gene="ggt"
                     /locus_tag="WM704_001869"
                     /pseudo
     CDS             complement(2238..4006)
                     /gene="ggt"
                     /locus_tag="WM704_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(4174..4437)
                     /locus_tag="WM704_001870"
     CDS             complement(4174..4437)
                     /locus_tag="WM704_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HFA9341221.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            4631..4849
                     /locus_tag="WM704_001871"
     CDS             4631..4849
                     /locus_tag="WM704_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFA9341222.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(5008..5982)
                     /locus_tag="WM704_001872"
     CDS             complement(5008..5982)
                     /locus_tag="WM704_001872"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFA9341223.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
BASE COUNT         1549 a         1388 c         1541 g         1661 t
ORIGIN      
        1 gttacggttg tttgagtggt gaaaattaac cttggagggc aggatggaag tgcatgacaa
       61 gattcggacg ttaagggaag tcaatcagtg gacgcaggaa gagatggcgg aaaaattgga
      121 aatgtcggtt aacgggtatt cgaaaataga acgcgggaaa agcggtatca atctcgacaa
      181 gctgcgccag attgcccaga ttttcaatat cgatgtggtt gagctgctgg cggagcagaa
      241 ccgatcgttt ttcttctcta tcggcgacaa taccaataac catcataata ttatcggctc
      301 tgatgaaatg ctggtgtttg aaaatgaaaa attgaggtcg ctgctggatg caaaggatga
      361 attgatcagg cagaaagata gtgaaatcgc agtattgaaa aagttggtca ttttgctgga
      421 agagaaaaaa tagcaatgtt accgaaaaca aaaaatgccg tctggaaaat gttcagacgg
      481 cattttgtat ggaaacatcg ggcgtttagt ttccgtaaac ggggtatttg tcgcacaagg
      541 cagtcacttg tccgcggact ttggcgaggt tggcttcgtc ttcgggatta gccaatacat
      601 cggcaaccaa gttcgacaat acgcgagcgt cggtttcgtt aaagccgcga gtggtcatgg
      661 cggcggaacc gatgcggatg ccggaggtaa cgaacggttt ttccggatcg ttcgggatgg
      721 cgtttttgtt gacggtgatg tgcgctttgc ccaaagcggc ttcggcggct ttgccggtga
      781 ttttcatcgg ttgcaggtcg acgaggaaaa cgtggctttc ggtgcggccg gaaacgatgc
      841 gcaaaccgcg tttaaccaac tcttccgcca tgacggcagc attgattttc acttgttttg
      901 cgtattgttt gaactcgggt tgcaacgctt ctttaaacgc tacggctttg gcggcgataa
      961 cgtgcatcaa cggaccgcct tgcaggcttg ggaagatgga agagttcagc gctttttcgt
     1021 gggtattgtc acggcacaaa atcacaccgc cgcgagggcc gcgtagggtt ttgtgggtgg
     1081 tagtggttac gaagtcgcag aatggcacgg ggttaggata ttcgccgccg gcaaccagtc
     1141 cggcatagtg cgccatgtcg acaaagaggt atgcgccgac tttatcggcg atttcgcgga
     1201 attttgccca gtcgatttgc aacgcgtagg cagacgcgcc cgccacaatc attttgggtt
     1261 tgtgttcgag cgcgaggcgt tcgacttcgg cataatcgag gacttcgttt tcatccaagc
     1321 cataagtaac ggcattgtag agtttgccgg aaatattaac gctcgcgccg tgggtcaggt
     1381 gtccgccgtg cgccagagac atacccaaaa tggtgtcgcc cggttttaaa acggaagcat
     1441 atacggcttg gttggcttgg gaaccggagt gcggttgaac gttggcatag gctgcgccaa
     1501 acagttcttt tacgcggtcg attgccaatt gttcgacaat atcgacgtat tcgcagccgc
     1561 cgtagtagcg ttttgcggga tagccttcgg cgtatttgtt ggtcaattgc gaaccttgtg
     1621 cctccatcac ggcgcagctg acgtagtttt cagaggcaat cagctcgacg tggtcttgct
     1681 ggcgtcggtc ttcttgggca atggctgctg ccaaatcggg gtcatattgt gcgagggtaa
     1741 cgctttttga aaacatattc tcggctcctt tgtgtaatca gggtatcatg agcgtttttc
     1801 gtataaaaaa atacttcaaa acctaaggca gatagcccat aatgcgtaaa ttttctttgg
     1861 cattatcagg taatttattt aacatgccgg tttttagcgt ctcatcacct ttgtttagca
     1921 caaggctaat caaaggcaat acatcaaatg gtaaattttc ggcagtttgt gcaaaatcaa
     1981 tcaaataacc agcaccaacc acatctttat cagccataac ccgctgatac accacttgcc
     2041 aagacaattc ttcctgcccc gccatgtctt aaaatcagat gaatggcttg taatgctgtg
     2101 tttggcgtct cacaaattgc caagatctgc tcagaagctt gcttgatggc ttgtattttt
     2161 gtttgattac tcacaatttc cccctatttt aataattaac ttaatgcggt caaattcaca
     2221 aaatacaagc ttcacctcta atcacccgtc actcgacctt ctcggcgtgg atcgacacca
     2281 ccaaccagcc tgcttggctc gataataatg gcttgaacac ctgaatttag ctcacgcaca
     2341 tcagtcttat agcccaaatc atttaatgtt tgttgccact ggacggcggt tgtacccgtt
     2401 tctagttcat agctaccaaa gcgatttaat aaattgggtg cactgatggc atcttggata
     2461 tccatattcc agtcactatg tgccacaatc gtcttagcga catagccaat gatacggcta
     2521 ccacctggag agccgattgc catataaggc ttgcctgctt taaatacgat ggttggtgtc
     2581 attgaggagc gtggtctctt gccaggctcg acacgattgg cgacctgttt gccctgcttt
     2641 attggctcaa aactaaagtc tgtcagctca ttattcagca ggtagccatt tgccatcaaa
     2701 gttgagccaa acgcattttc aatggaagtc gtcattgata gcacattgcc cgccttatcc
     2761 acaattgata tatgactggt agaaggtaac tcaatcgctt gtgaggacgc ccactcatga
     2821 ataaaatcgc ctgcagatac gctaggcaat gccttatccg actgctcaag cagctggctg
     2881 cgatgtttta ggtagtcttt agaaatcaac tggcgaatgg gtactggtac aaaatcaggg
     2941 tcgcccaaat atacatcacg atccgcaaac gcaagcctag aagcatcgcc caagagacgc
     3001 aaaccttcag catcataccc cacccgattg ggtgaaaatt catttaaaat ccccaaaatc
     3061 tgacccacag caatcccacc tgagcttggt gcacccatac cgcatacttc ataaatacga
     3121 taagtcacac aaacaggcgg gcgttccacc acttgataat cagataaatt ttgcaaggat
     3181 aattgaccgg ggttatcctt agcattttgg acaactgaaa caatattttg ggcatattta
     3241 ccagtatgca gagcttttgc accttgagct gctaacgcct gaacactgtc agcaaattct
     3301 aaatttttca gcaagctgcc tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct
     3361 gtttttggat agcgtgccaa atgctgctga ttttgctcaa ccgagatggc aagccttggc
     3421 gacacctcaa agccttgttt tgccaagtgg atcggcgtat caaataattt tccccaaggc
     3481 aatacaccgt atcgctgatg tattgtctcc gtcagtttag ggatagcagg cgtacctacc
     3541 gagcgaccac cgcttccata aatttcaatg gttaaccatc tttatccaaa aataattctg
     3601 gcgtcgcacg catcggtgcc gtctcacgcc catcaaatgt ggtcaatgtt ttggcggtat
     3661 tgtcccaata caacacaaat gcaccaccgc ccaagcctga cgactgtggc tctaccaagc
     3721 ttagtgtcgt ctgcaccgcc accatcgcat ctgcagcgct accgccttgc tttaagatat
     3781 catagccagc ttgtgttgct aatggattgg ctgacgctac tataaaatca cttgcaatca
     3841 cctgcttttg ttcggtcagt cccgttacat gttcaggcgt gtgagcgtct gcacctgcga
     3901 tgacagcaga attagtatta accttacctt gattggcatg gatgacttga catccggaga
     3961 ttaccataga cattatcaat gcagtcaata aatatgtttt agccacaagc actccttcgc
     4021 ctgagtttga ttgataattc atacaaagca tgctgattat tgtatgtaat atggctaaat
     4081 aattcaatcc aaactatcaa tcttgaccat caaaaaaaga ccgctaatgt catcagcagt
     4141 cttttttgat atttatttta agatattaag taatcagacc tttgggctat gctcttcaat
     4201 gagtggtttt agctcacctg attggtacat ttgtaggata atatcactac caccgattaa
     4261 ctcaccatta acccaaagcc gtggaaaggt tggccaattg gcgatgagtg gtagagtact
     4321 gcgaatttct ggattttcta ggatattaac aaaagcaaag ggtctgccaa tttgggtcgg
     4381 cacctctact gcgcgcgctg aaaatccaca ttggggaaac tggggcgtgc ctttcatatg
     4441 cagtaggaag ggctgaaaat ttatgcgtaa agcggaagat tgtcaacgat tttatcggac
     4501 ggtggggcgg ttttctgcgg acggtgttgc cgtgtataac aaaacactgg ataaaaatat
     4561 tatctttgtt ataattaatg taaagattca atttgacttt ttaaccgtaa accaagagag
     4621 gaaagcgatt atgttcccag aataccgtga tttgatttcc aaattgaaac aggaaaattc
     4681 ccgcttcgcc cgtctgttcg acgagcataa cgagctggac gataaaatta ccggtctggc
     4741 caacaatccg gttaccagcg gtgcggaaac catcgatgag ctgaaaaaag ccaaattgaa
     4801 actgaaagac gagttgtacg ccatcctgca aaaagcggcg ggcaaataaa tttgagctct
     4861 aatttttaaa ataccgtctg aaaatttttc agacggtatt tttatcgttc ggaaaggcag
     4921 ttttctgttt ttccggaggg tatgcttgaa atatcggcgc agtatcggat tcacgctgcc
     4981 gccggtcttt atcagtacgg tctctgccta ttttgaatgc agacggttga ggtaatccac
     5041 ttcttcgctg ctgcccataa tgacggcgac gcgttggtgc aggctttcgg gctgtatatc
     5101 gagcatggct tgatatgcgt tgcttgccga tgcgcccgcc tgttcgagta tcagggccat
     5161 agggttggct tcgtacatca ggcgcagttt gccgggtttg gacgggtcgc gtttgtcttg
     5221 cagatacatg aacacgccgc cgcgcatcag gatgcggtgg atttcggcaa ccatactggc
     5281 tacccagcgc atattgtagt ttttgccgcg cgtaccggtt tcgcccgcca agagctcgtc
     5341 gacgtattgt tgaacggggg gtagccagtg gcggcggtta gacatattga tggcgaattc
     5401 tttggtactt tcgggtactt tcgggttttc tttggtcagc acaaattcgt tttcggcatt
     5461 gagtgtaaat acgtacacgc cgtgtccgaa tgtgaatacg agctgggttt gaggcccgta
     5521 gaggacatag cctgcggcaa gctgctgtct gcccgtttga aggaatgatt cggttgccaa
     5581 tgcgccttcg ggtttggcga gaatggagaa aatcgtaccg acggaaatgt tgacatcaat
     5641 attggacgat ccgtctaaag ggtcgaatag gacgagatag cgtccgtttt caccggcact
     5701 tacgaaagtg tcttcttcct cgcttgccaa accggcaacg gcagggttgg ctttgagtgt
     5761 gtcaatcata atgttgttgg cgataacatc cagttttttc tggtcttcac cctgaatatt
     5821 gcccgtgccc gccataccca atacgccggc aagtgcgccg aggcggactt tggcgttgat
     5881 ttcggtacag gcggaaacga cggacagcaa aacgccgccg agtgcttcgg gcagttggtt
     5941 ttgttgcagg tgttcgggga ggaatcgggt cagtgtgtcc atggtttgct cgtttcggaa
     6001 aggtttgtgc cgtctgaaag gcggcagatt gctgcggggc gttcctttgg tgcgtttcgc
     6061 cggatatcgc gtgggattgt agtggaaaaa aacggcgcgg ggcaagtcgg aacgcggcag
     6121 gcggatcgag aagtacaag
//
LOCUS       DAWTHD010000114         5963 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWTHD010000114 DAWTHD010000000
VERSION     DAWTHD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.114"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(385..470)
                     /locus_tag="WM704_001873"
     tRNA            complement(385..470)
                     /locus_tag="WM704_001873"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Leu,seq:gag)
     gene            complement(484..834)
                     /gene="secG"
                     /locus_tag="WM704_001874"
     CDS             complement(484..834)
                     /gene="secG"
                     /locus_tag="WM704_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFA9341224.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(841..1614)
                     /locus_tag="WM704_001875"
     CDS             complement(841..1614)
                     /locus_tag="WM704_001875"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFA9341225.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            1773..2300
                     /locus_tag="WM704_001876"
     CDS             1773..2300
                     /locus_tag="WM704_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFA9341226.1"
                     /translation="MYANMGDMMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWLWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGEL
                     AKILERRFEASSQAA"
     gene            2403..3059
                     /locus_tag="WM704_001877"
     CDS             2403..3059
                     /locus_tag="WM704_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFA9341227.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKIVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            3140..3463
                     /locus_tag="WM704_001878"
     CDS             3140..3463
                     /locus_tag="WM704_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFA9341228.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(3726..5893)
                     /locus_tag="WM704_001879"
                     /pseudo
     CDS             complement(3726..5893)
                     /locus_tag="WM704_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
BASE COUNT         1053 a         1343 c         2027 g         1540 t
ORIGIN      
        1 atctaacttt tccccggtaa caaggtaaca actaagggta tcgcttccta gtataggttt
       61 tttccaaaaa cccaaaaaaa ccgctctgca tcttggacgg tttccccctc gttccctacg
      121 gttcgtcagt attccccgca agctcttttc cgcttggcat tcctgatttg gtggtcgttc
      181 agctttattt ttgcggtatt tttcggatac cgtaaaaaat caggttagtt caattagggc
      241 ggattggacg ggattgcaca gtcaggaagg ggacggcagg ataacaatca gcctgaaatc
      301 cttgtattga ttgggattgg ttgatggcat cagacaggat taaacgaaaa aaaacgccta
      361 gaatttctaa gcgtttttgt gtgttggtgc cgacagcgag atttgaactc gcacagccta
      421 cggccactac cccctcaaga tagcgtgtct accaatttca ccatgtcggc atttgaaaaa
      481 ctgttatttc tgctgctgag gaacaggggc agaaggttcg gtattgctta cgggtttggg
      541 tgcttgctga gtctgtcgta tgttgctgaa gtccaaaccg tgttttgtcg tgtgggtgtg
      601 aatatacacc atagccatgc aggttgcgaa gaaaaatgtt gctgcaacgg cggtcgagcg
      661 gctgaggaag ttggcgttgc cggcagagcc gaatacgcct tgcgcgctgc cgcttcccga
      721 tccgaaggtc gcgccggcat ccgcgccttt gccgtgttgg agcaatacta acacgatgac
      781 ggccaaagcg gaaataatat taataatcca aattagggtt ttgaaggctt ccatattttt
      841 ctacgcattt tgtgcggcac tgatgatggc ggtaaaggag tcgtacgaca atgacgcgcc
      901 gccgacgagt gcgccgtcca cataaggtac tgcgaagatg tcggccgcgt tgtccgcttt
      961 cacacttccg ccgtaaagga cgcggatttt aacatcgctt ccgcacaaag acaagatttc
     1021 tttgtagatg aatgcgtgca tatcggcaat ctgttcgacg gtggcgactt tgcctgtgcc
     1081 gattgcccag acgggttcgt aggcgacggc gatgtttttg gtatccagcc cttgcaggat
     1141 ggaaagctga tgggcgatga cttcgtgttc tttgccggtt tcgcgctctt cgaggctttc
     1201 gccgacgcac aataatggga tgagtccgac gttgaggacg ttttccattt tgcggcgttg
     1261 gatttcgttt ttttcgccga aatagaggct gcgttcggag tgtccgatga ggacgatgtc
     1321 tgttccggtg tcggcgagca tttcggcgga cacttcgccg gtgtacgcgc cgttgtcggg
     1381 gaagcggctc acgtcttggg cgcaggtgag gatgcggttg tttaagacga tttgcatggc
     1441 gttgtgcagt tgcagcaggt aaacggtcgg ggcggcgagt ccgatgagga cgcgttcggc
     1501 ggtggggagg atgcggaagc ggtgcatcag tgcgttgttg ttttggagcc ggccgttcat
     1561 tttccagttg ccgatgaccc atttttgatc ccacattccg atttggcgat acatcttttt
     1621 tgctccgtgt cgtgtttttc tgtctgccgc gtgtggcgcg ttgcaatgtg cagtttagtg
     1681 gatatgcggc gggttcgcaa cttggggcgg gcggctgcgg gggcggtttg gaatgttgtt
     1741 tcgggcagga tgttttataa tggctgcctg atatgtatgc aaatatggga gatatgatgc
     1801 acgcgcttca tttttcggct tcggacaagg ccgcgcttta tcgtgaggtg ttgccgcaga
     1861 ttgagtctgt ggtggcggac gaggcggatt gggtggcgaa tttggcgaac acggcggcgg
     1921 ttttgaagga ggcgttcggc tggttgtggg tgggttttta tttggtcgat acgcgttcgg
     1981 acgaattggt tttggcaccg tttcagggac ctttggcgtg tacgcggatt ccgttcgggc
     2041 gcggggtgtg cggtcaggct tgggcgaagg gtgaaacggt ggttgttaag gatgtgaacg
     2101 cgcatcccga ccatattgcc tgttcgtctt tgtcgcgttc ggagattgtc gtgccgctgt
     2161 tttcagacgg ccgctgtatc ggcgtgttgg acgcggacag cgaacatttg gcgcagtttg
     2221 atgaggcaga tgctttgtat ttgggcgaac tggcgaagat tttggagagg cggtttgagg
     2281 cttcgagtca ggcggcttga gactggcaaa acgggcgggc ttcgcgtgcc gaagttggcg
     2341 cggcggtagt gtggttttat aatgcctgcc attgataaaa caattatttg acggagcact
     2401 aaatggattt tgaaaaagcg cggttcaata tggtcgaaca gcagatccgt ccttgggatg
     2461 tattggattt tgacgtgttg gacgctttgg aggagattcc gcgcgagctt ttcgcggatg
     2521 agtctttgca gggtttggcg tatgcggata tggagctgcc gcttgccaac ggtcataaga
     2581 tgctcgagcc gaaaatcgtg gcgcggctgg cgcagggctt gaagctgacg aaaaacgata
     2641 cggttttgga aatcggcacg ggttcgggct atgcgacggc tctgttggca aaactggctg
     2701 gccgtgtggt ttcggacgac atcgatgccg aacggcaaaa gcgcgccaaa gcggttttgg
     2761 acggcttgtc tttggaaaac atcgattatg tgcaaaataa cgggttgacc gaactttccg
     2821 caggtgcgcc gtttgatgcg gtttacgtcg gcggcgcggt aacccttgtg cctgaagtgt
     2881 taaaggaaca gttgaaggac ggcgggcgta tggcggttat tgtgggacgc aggccggtgc
     2941 agcgcgcgct tttgattacg cgcaggggcg atgtgtttga agagaaggtg ctgttcgata
     3001 ctttggtggc gcatttggac gacaaggatg cccatccttt cgacagtttt aatttttgat
     3061 gttcggattg tgatgccgtc tgaaagcggg tttggggctt cagacggcat tttgcttggt
     3121 ttttttcggg gggtttgtga tggatattgt gcaacttccg tcggcggcat tgaaggcgtg
     3181 gatggacgaa gggcggatgt tttgtttgtt ggacgtgcgt acggatgaag aagcggcggt
     3241 ttgttcgctg ccaaatgcgc tgcatatccc gatgaatctg attccgctgc ggcaaaacga
     3301 gttgccggac gatgtgccgc ttgtggtgta ttgccatcac ggtatccgca gcctgcatac
     3361 ggcgatgtat ttggcggagg cgggttttga aaacctgtac aaccttcagg gcggcatcga
     3421 cgcgtgggcg gttgaggttg acgcggaaat ggcgcggtat tgaaggcggc ttcagacggc
     3481 attccttaaa tgcgtgtacc tctggtgttc cataaaggtc gtctgaaagt gcagcttctg
     3541 cgaagctaaa gcgcagtatc aacgaagtta aaatttgcct gaaccttaaa ggcagcctgc
     3601 accccaattc cctcgccccg tgggagaagg ctagggagag ggcggcaaac tgcaggtttg
     3661 cttgggcggc attttcaatg tgcaggctgc ttttagcttc gcagaaactc cgttttcaga
     3721 cgaccttatt taaaccgata ggtaaacgcc gcgttcactg tccgcagtgc gccgtggctg
     3781 tggcggtcgg gctgggtgcg gtagtgtttg ttgaacaggt tgtccacgtt cagcgacagt
     3841 tcggtgcgcg gattgaagcg gtaacgcgcc atgatgtcgg cgacggcgta ggctttctgg
     3901 cggctgttgg cgacggcgcg ggctttggcg gcggggttgg ggatgtggag cgcggctggg
     3961 tcggtgtggg tttcgccctg ccggcgcaca cccgcaccga tggtccggcc gctgggggct
     4021 tcgggggcta agtggtaggc ggtgaagagt ttgaagctgc gttcgggtac gctgtcgggg
     4081 ttcaggcggc tgccgtcttg gtcgcggggt ttgctttggc tgtagcctgc ctgtatctgc
     4141 cattcgggcg tgatgcggcc gccgacttcg atttcccagc cgtgggtttt ggcttggttg
     4201 gcggcgcgat agtaggtgtt gccgctctgg tcgcgtcctg ctgcggtggc gaggttgttt
     4261 ttacgggcgc ggtacacggc ggcggatgcg ttcagacgcc cttcaagcca ttcgcctttg
     4321 atgtcggctt ccagattgtt gccggttacg ggtttcaggt agctgccgtg ttcgtctttt
     4381 tgcaattgcg ggacgaacag gctgctgtac gagccgtaaa gcgacaggtt gccggtcaga
     4441 tcgaacacga tgcctgtgta gggggtgaaa cggttggcgg acacataggt catgccttgt
     4501 gtgcggctgt tgtagctgcc tgcgcggtag cggctgtatc tgccgccgag tatcagcgaa
     4561 aggttgtcgg cggcgcggaa acgggtggcg agatagccgc cgatttgccg cctggtgtcg
     4621 tattgcggga tggtttgggc aaacgatgat ggctgcggat aggcgcccgt gcgggaaaat
     4681 tcgtaggcgt tgggaatggc gttgggaatg atgctgcgtt cgccgtattt gttgctggcg
     4741 tatttgtagc cgttgatacc cgcgattaaa tcgtgctcgc ggccgaacag gcggtatttg
     4801 ccggtcaatg acatgctggc gctgtgggtg cgcggatcgg cgtgccaata accgggaatc
     4861 aggtcggtgg cggcagtgct gtggtcgatg gaaagtacgc ctgccacacc gtagggctgg
     4921 cggaagcggc tacgggtgta gtcgtattcg gctttgagtt tccagtcttg gttgaagcgg
     4981 tgttctatgc cggcgaacag gttgagcgca cggttgcggc tgttcgacca atttgtggcg
     5041 gggttgtctt ttgggccgaa ggcggtggca taaccttggc tgtcgtacac ggcgtagctg
     5101 agcggcgcgt ctgcggtttc tttcgcctgc tggtagtcca tgcctgcgtg gacgcgggtt
     5161 tgcggtgcga tgtcgtattc caaaatgccg tagagttcgg catcgcggct gcgttcgagc
     5221 tgccgccacg agtcgccgcg tccgaaggtg gaaaccaggc ggccgcgcag cgtgccttcg
     5281 gcgttcaggc tgcccgatac gtccgcgccc agcccgaaat gtttgcggtt gccggcttcg
     5341 gcgcggactt caaacaatgg cttgcgggtc gggtgtttgc gtaccagatt gacggtggca
     5401 gaaggctcgc ccgtgccgtc cggcagcccc gccacgccgc gcacgacttc cacgcgctca
     5461 taggcggcgg tgttggcatt gcccgtatcg gccagcgcgt cggcaacggg gatgccgttg
     5521 atttggtagt tggcgatgcg gctgccgcgc gcgaacaggt agttgtagcc cgcgcggtcg
     5581 gagccgggct ggtgccggtc gcctgcaaca gggcgcggtc gagcgttttg atgttttggt
     5641 cgcgcatttg ttgcgatgtg atgacgctga cgctctgcgg gatttcgcgc agggtcatgg
     5701 gcagcccgaa cggggtgtgc gtgccggaaa cggtgtagcc gtcgttggaa ctcgcggtgc
     5761 ggtcggcggt aacggtgatg gtcggcaatt cggtgctttc ctgcggtttg gggtcgtctg
     5821 aaacagaaac atcggcctgc gcgtacacgg gtagcagggc ggcaaaaagc agggagtatt
     5881 tgaagcgtgt catggggttt cctttgtgtg ttggtgtgcg gtggggttgt atagcggatt
     5941 aactttaaac cggtacggcg ttg
//
LOCUS       DAWTHD010000115         5864 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWTHD010000115 DAWTHD010000000
VERSION     DAWTHD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.115"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..3179)
                     /locus_tag="WM704_001880"
     CDS             complement(<1..3179)
                     /locus_tag="WM704_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFA9341229.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLTYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAGYEQIHRALLTGL
                     IANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     DFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSASPLPNPLPQERE
                     QSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPPFSDGLRPANPQQ
                     TTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDDPKPKKQPASQKG
                     RLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKHWQTADGK
                     FKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQLQIKALPKQIRR
                     ICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQDEWAAFRLPEHC
                     YFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDNVTAWDIGILPES
                     IKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIELMKLQLKEQVKDL
                     NKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEKAFKEQIKRARSR
                     LPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWAQWPR
                     LPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQGQPVSDGLAAFK
                     WMIEELRVSLFAQELKTPYPVSVKRLLKM"
     gene            complement(3281..4858)
                     /gene="lpt3"
                     /locus_tag="WM704_001881"
     CDS             complement(3281..4858)
                     /gene="lpt3"
                     /locus_tag="WM704_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFA9341230.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSMIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIDKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            5319..5471
                     /locus_tag="WM704_001882"
     CDS             5319..5471
                     /locus_tag="WM704_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341231.1"
                     /translation="MSNKQANHQSNMSNANKGTSGTNKTYSQNQGNRGWQMNPQNPAN
                     SGKGKK"
     gene            5542..>5864
                     /locus_tag="WM704_001883"
     CDS             5542..>5864
                     /locus_tag="WM704_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341232.1"
                     /translation="MENKQTEKTETQSEKTKKKPFKQTRELVRLALNNGWTQTEIAKT
                     CRTQQSVVSAWSKGEKLGTEQQLKPLLDLFGYKLRRNTFKLYWRKNAETSKIEFIKVE
                     GKIIF"
BASE COUNT         1347 a         1332 c         1658 g         1527 t
ORIGIN      
        1 cacatcttca acagccgctt taccgacacc ggatacggcg tcttcaactc ctgcgcgaac
       61 agcgacactc tcaattcttc aatcatccat ttaaacgcgg cgaggccgtc tgaaacgggc
      121 tgtccttgtt tcgccaagcc gtcagttttt tcctgccaca tttgttccag ctcttggata
      181 tcggcttcgc gggctgcgtc gcgggcgggg ttgctgctgt atttttcgag gcgcagggtc
      241 atggctttga ggtagatggg gaggcgcggc cattgtgccc acggggtgcg ggtggcgaag
      301 ccgggtgcaa gcagggtttg caggcgtagt cttaggaggt gggtcaatgg gtgtttgccg
      361 agtttgccgt tcagttcggc gtaggcggct gcggtttcct gcaggtagcg gctgagggct
      421 tctttgacgg cgggcaggcg gctgcgggcg cgtttgattt gttctttgaa ggctttttcg
      481 ttgcgcggca gcttgtcttc gccgataaag gcgcggtcgc agacggcttg ggtgaggtcg
      541 tcgcgcaggg tgtcggcgtt gatgtgtttg agcagcatgg cggcttgggt gaagccttgg
      601 atgcctttgt tcagatcttt aacttgttct tttagttgta gtttcatgag ttcaatcacg
      661 cctaatctgt gggcgtgtcc ggcggcggca gacgtgtcga acaggcgcag ggcgatgcgg
      721 ccgtcttttt ctttttgcag gccgaggtag ccggtgagct gttgtttgcc gcgggcgaat
      781 ttgatggatt cgggcaggat gccgatgtcc catgcggtaa cgttgtcacg ctcgaattct
      841 tgggtgttgt cgcggaaggt ggtggtggcg gctttgccaa gttgttgttg gatttggatt
      901 aaatcgcgac ccatggctaa ctcttgtccg ccgtcgtcga tgatgcggag gttgaaatag
      961 cagtgttcgg gcagcctgaa cgcggcccat tcgtcttggt tgatttgctc gagtatgcgg
     1021 atgtcgcctg cggttttggc gatggcttgg gcgagctggg gcaggatggg ggcgttgcgg
     1081 tcggggtttt ggcttaaaaa ttgggtgatg aattcgggta cgggtacgca gatgcggcgg
     1141 atttgtttgg gcagtgcttt gatttgtagc tggatttttt cgcgtatcat gccgggcacg
     1201 agccattcga gggcggcggg gctgatgcgg ttgagcacgg tgagcggcag ggtgagggta
     1261 acgccgtcga gcgggtggtg cggctcgaag cggtagctga gtttgaattt gccgtctgcg
     1321 gtctgccagt gtttggggaa ttgttcttcg gtgatgtgcg cggcggcgtg ctgcatgagg
     1381 tcgtcgcggc tgaggaacaa caggcgcggg ttgtcgcgct cggcggtttt gagccaggct
     1441 tcaaaggtgc ggatatcggc aaggggcaaa ggtttcagac ggcctttttg agatgcaggc
     1501 tgctttttgg gtttggggtc atctgaaact gttaaagccg aggcactctg ttccctctcc
     1561 tgtgggagag ggtttgctgc ggttgcggaa aagttggttt gtgcggcaac tgttttgccc
     1621 tctccccagc cctcccccac gggggagggg gtggtttgct gggggtttgc aggacgcagg
     1681 ccgtctgaaa atggcggggt gtagttttca gatagccttt gggagtgcag gctgctttcg
     1741 gttttagttt tagcttcgca gaaattcagc cttgcttcgc aagacattgt tttcagactg
     1801 cctgaaaccg ttgaaactga ggcactctgt tccctctcct gtgggagagg gttagggaga
     1861 gggcttgctg aggttgcgga aaagttggtt tgtgcggcaa ctgttttgcc ctctccccag
     1921 ccttccccca cgggggaggg ggtggtttgc tgcggatttg caggacacag gccctctgaa
     1981 accgaatccg ccatataaaa atcgggcagc cgttcgtgat aaaacgcaaa cagggcttcg
     2041 tcatcgacca atacgtcttg cttgcgcgat ttgtgttcga gttcggtaat ttctttaatc
     2101 agctttttgt tgtggacaaa aaaatccgct ttcaaatcgt attcctgcgc caccaacgcg
     2161 ctgcggataa agatttcacg tgcttcttcg ggcgcaactt taccgtaaga cacggggcgg
     2221 cgcggcaata cggtcaggcc gtaaagcgtt acgcgttcgc tggcgacgac ttcgccgcgt
     2281 ttttgttccc agtgcggctc gaaataatgg tagcggacaa ggtgcggggc ttcctgctcg
     2341 atccattcgg gctggataac ggcgacgtcg cgcgcgtaaa ggcgcgtggt ttcaaccaat
     2401 tctgccgcca tcacccattt gggcttggct ttgaacaggg cggaggcggg gaaaaggtgg
     2461 aagcggctgc cgcgcgcgcc ggtgtagtcg ttgctgtcgg gcgatttcat gccgacgttg
     2521 gcgatgaggc cggtgagcag ggcgcggtgg atttgttcgt agcccgcttc tttggtagcg
     2581 cggatttggg tgcggtgttg tttcttatcc agttgttttt gtttgagttt ggcagatagg
     2641 tcttggtcgc ccgcattctc agacgacgtc agttgcttga cttcgggagg tcgtctaaac
     2701 gcagcttctt tggtggtcaa gcccatttca atcgcggttt gggcaagctg gtggtgcagc
     2761 tcgcgccact cgcgcatcct caggtgcgac agaaaatatt gacggcacca ttgcaccaac
     2821 tgcttgttgg acaaaccttt gtcgcgctcg cgctggaaac tgtcccaaat attcaggtag
     2881 gtaaggaaat cggattgctt gtcggtaaag cgttcatgcg ccttggccgc ggcatcgcgc
     2941 gcttccagcg gccgttcgcg cgggtcttga atcgacagcg cagacgcaat caccaatatt
     3001 tccgccatgc agtcgtgttt cttcgccgcc agcaaaatgc gcgcaatttt cggatcgatg
     3061 ggcaggcgcg ccatttgttc gccgagcttg gtcaggcggt agcgcgtgtt ggtaccgata
     3121 tggcccgaac tgttttgcag gccgtctgaa acattggccg ggttggatcg ggcatccatg
     3181 cctgatccgc gtacttggtt tttcatattt tgcatagtct tgtcggtcgg gcatcttccc
     3241 cgacatcatc taaatttgtc tttattggtt ttcacgctgc ttattgcgga taaacatatt
     3301 ccgccttgcc gttgcgaatg ttcaagctgc ctgcatcgcc cgtaatcagg ttgcctgtta
     3361 ccgagccttc gcgacaacct gaaaccggca tatcgtagcc caacgtgtga atcaggaacg
     3421 ttgaaagctg ctgatggaag gcaatctcgc aaggcgcaaa agcctggttg gcagcctgtt
     3481 gcacggcctt atccgggctg tacaaaacca gaggcacaat atagctgtcg ggctgcaccg
     3541 tgccttgatt gtagatatct tggcgcacat actggccatg atcggaggta taggcaaaca
     3601 gccagttgcc gtcaggctgc ttttgcagct gctcgaatac ggtttgaatc atttggtcgg
     3661 ttttgtggat ggtgttgtcg tacttatccg caatatcggc ttcgccgaat actttatctt
     3721 gaggctgcaa caatgcgccg tatggggcgt gcgaaccgcg ttggtgcaac acgataaaat
     3781 gcctgccctg ctgcaaattg attttgtcga acaacggcag cagcttctca tcgggcatat
     3841 tgtcgccgtt gccgtagcca agttgcgtcg gctgaatcag atggtctatc catttcttat
     3901 cgattaagtt caaaattgcc atttggtttt cagcctgggc actgtaaaaa tacgtttcat
     3961 agccctgctc tttggcgagg cggaacatat tggtatcgcc gccgctgatt tgttccaagc
     4021 cgttggcgtg cggtatgacg ttaaagaaac tgggcaggga tactgccgtc ataaagcctg
     4081 cggaataact ttgtttcaca atcggcttaa aatcggcttg cgacagccgg gttaaaaacg
     4141 gcgaagtttc gcgcccgtaa ccaaacaatt tcaaatgcgc cgcgctttcg ctttcgccca
     4201 taatcaggac gatattttga atactgcctt gcccgatttt gcttggagca ggctgtttga
     4261 acacagggat cttgcttaaa tcaaacaact gatacggcaa cacgcgtccg acaaaataac
     4321 cgaagctgaa ataattggct ttgatgcggc tgtatgtcgg tttgggcgaa ataccgtgct
     4381 cttgtttcgt gtcgaacgaa cgcacgaaaa tcatcagcat taggaaggca aacaggatat
     4441 cggcagaaaa atgcgtctta cggcggaact tggcaaggct gcaaaacagc acgacttccg
     4501 ccacgcccca caaagcaggc agccacaact tatccaacat cgacgcgccc gcgctgccga
     4561 cttcggtaac ctctttcagc atcagccaat aattgatgcc cgtcatccag ctttgataaa
     4621 ccgcgtaatg cacattgttg gcaatcatgc tgaacgcgaa aaacaccgca atcagcagcc
     4681 gcgaagcctt ataacgcgca aacagataca gcgcagcaat cataaatgtc agcgcaaacg
     4741 tttccgccat ttttgcagcc ggtaaggttt caattccgaa tacaaagcga taggcgattt
     4801 cgctggcggt aagtagggat gaatacagaa agagaacgaa aagggatttt ttcataatgg
     4861 gcagttcaca ataaaagttg caggacgggt tttggggctc ggcaacggta ttcttcagca
     4921 gctatctgtt tacagattgt ttttcaaaaa acgagaagta aatcttaaat ccatagaatg
     4981 tctatcttaa aatcattaaa tatcaataaa ttacaaaata tcataaaata tcaaaattta
     5041 tcaacttttt atcaatggaa cttttgctac gaatttttta tgatgctacc tatcgtcatg
     5101 gggggtaatc atgaaaaaat ttagcaccaa ttcaaaaatc aattctttta ttcattcact
     5161 actaaatagg gggtggaaaa tacaaaatag aaaaaagcac aatattttgg tagcaccaaa
     5221 taaccgtaga attgcaatcc catcaacacc gagcgattgc aaagcaatcc acagtttcac
     5281 caagcagatt aaatatcttt ttcaggagta gaactaaaat gagcaataaa caagccaatc
     5341 atcaatcaaa catgagcaat gctaataaag gcacgtcagg aacaaataaa acgtattcac
     5401 aaaatcaggg aaatcgtggc tggcaaatga atccacaaaa tcctgcgaat agcggtaaag
     5461 gcaaaaaata gccattgcct tgcctataaa tggcgatatt atacttggta tcgccatttt
     5521 aattggcata ttgggggtat tatggaaaac aaacaaaccg aaaagacaga aacacaatct
     5581 gaaaagacaa agaaaaaacc atttaagcag acgagagaac tcgttaggct ggcattgaat
     5641 aatggttgga cgcaaactga aattgctaaa acttgccgca cacaacaatc agttgtgagt
     5701 gcatggagca agggcgaaaa attgggaaca gagcaacagc tcaaaccttt attggattta
     5761 ttcggataca aattacgtag gaacacattc aagctgtatt ggcgcaaaaa tgcagagaca
     5821 tctaaaattg aatttattaa agtagaagga aaaataattt ttag
//
LOCUS       DAWTHD010000116         5468 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWTHD010000116 DAWTHD010000000
VERSION     DAWTHD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.116"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            127..378
                     /locus_tag="WM704_001884"
     CDS             127..378
                     /locus_tag="WM704_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341233.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDTLDRCSG"
     gene            complement(609..1490)
                     /gene="nadC"
                     /locus_tag="WM704_001885"
     CDS             complement(609..1490)
                     /gene="nadC"
                     /locus_tag="WM704_001885"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFA9341234.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARIAFQTMDPSVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAKVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTVHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(1566..2504)
                     /locus_tag="WM704_001886"
     CDS             complement(1566..2504)
                     /locus_tag="WM704_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341235.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            2706..3818
                     /gene="nadA"
                     /locus_tag="WM704_001887"
     CDS             2706..3818
                     /gene="nadA"
                     /locus_tag="WM704_001887"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFA9341236.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDDETAGRL
                     KDEIKALLKQKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            3870..5378
                     /gene="nadB"
                     /locus_tag="WM704_001888"
     CDS             3870..5378
                     /gene="nadB"
                     /locus_tag="WM704_001888"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFA9341237.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1171 a         1619 c         1583 g         1095 t
ORIGIN      
        1 ttccttttcc cactattttt gcaaacaagc gtaaagacag gctttcagac ggcataaaac
       61 tgccgcccct accctgcctc accctgttgc cgaacggaat catccgtatt ttttaagaaa
      121 accattatgg atattttccg tgaatcaagg ccgttctgcg cgacaggctg tccaacgcca
      181 acatccggtt cggcatttca tcatcgccgc cgacgatacg ggcaagaccg catcggtcgt
      241 gccttcctaa cgatgcaccg catcctcgac aaggaaaacc agatacatac cgatatggct
      301 ttagcgttgc cggcggtagt gaaagacgca gtggcgatat gggcagatat ggatacattg
      361 gataggtgtt ccggctgacc gctccgttac tgccgtatct ttcggctacc gttcatcaaa
      421 tggttccaat gccgaaatgc ctaaatcgcg cctgcctgaa atgctgtttt ttctaaagca
      481 tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattc cctccctgag
      541 gcaataaccg gattacggtt aagcggttcc ttacctgaca tatcagacag ccgcccgcac
      601 cctgaaactc acgccacgaa atccaaacct atgtccaacg atcggctgct gtgggtcaga
      661 tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg gtcgaagccg
      721 atgccgcccg atgcttcgca atagacggtg tgcgggtgga cggtttgtgt gtggcagcgg
      781 tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg ttctgcgccc
      841 gctgcgatgg cttcgtccag ttgtgccaat gtgtccactt cgatttccac gcaggtcaat
      901 ggtccgaccg cctgttttgc ctgccgaacg gcttgggcga tgctgccgca ataggcgagg
      961 tggttgtctt tgatgagcac ggcgtcgtcc aaacccatgc ggtggttcgc gccgccgccc
     1021 gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg gctgcacacg
     1081 atgtctgtac cgtattcggc gaccttggca acggcacgag cggtggcggt ggcaatgccg
     1141 ctcaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc gcgggcgttg
     1201 ccttcgacgg cagccagtgt ctgacctgcg cggacggctt gcccgtcttg gatttcggct
     1261 tggaagcgga cggacggatc catcgtctga aaggcgatgc gcgccaagtc cataccggcg
     1321 ataacaccgt cttcgcggct gacgaggaaa agtttggcgg ttttgtcggg cgcgatgacg
     1381 gcggcggacg taatgtcgcc gcgcctgccc aagtcttcgc tcaaggcttg ttccaccata
     1441 gggcgcaaca gggtgtcggg cagggcaaag agggggtttt cagacggcat ggggtttcct
     1501 ttgttaaaaa agattgatgt tgcaccgatg gggcaaaact tcatttgatg ccgtctgaaa
     1561 acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc ggacaggacg
     1621 ttgtcgcgga agcggtaaag ctgcgcggga cggcctttgc tgcccgatac gccggtatcc
     1681 gacggctcga taaggttttg ctgctgaatc tggcggcgga agttttgctt gtgcagcaat
     1741 ctgccgctga tggcttcgac gctgttttgc agttgcagca gtgtaaatcc gggcggcatc
     1801 agttcaaaaa tcacggggcg gtatttgatt ttggcgcgca ggcgggacag ggcggtcgcc
     1861 aacacgcggc ggtggtcgtg gcgcatggtc tgccccgtga gcgcgaagtc gaagtttgcc
     1921 tgcggctcgg cggcctccgc tatcagaccg ctttcataca acatttcata gcgttgcaaa
     1981 acgtattctt ccgaccagtt ttccggttcg accccccaac acaaatgaat atgcttgagc
     2041 cgcttttggc ggacttcctc cgtgtccgcc gagtttgccc aaatgcgcag gcggctgacg
     2101 acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc atgggaaata gccgtagcag
     2161 tcctgccatt tcgcgtcggg atgcaggatg ctatcggctg cctcgcgcac cagccccaaa
     2221 tagctgacgt acagcacggg catgccgtgt tcgttgcggc ggtgggtatc gacaaaggtg
     2281 taaagctgtt ccacatagcc cataggctgc gaagtctgct tggcgaccca cagctttacg
     2341 cccgcctgca gggaattgcg caggggggaa agcgggccgt tgggcaggag cgtgccttgg
     2401 gcgacggtca acacccgcag gccgccgtcg gtaacggcaa tcaatacggg aaccagctcg
     2461 acgatgcttt gcagcggagc ttcggcttcg gggtaggcgt ccatgctcgg ctttctgcgg
     2521 gtgggaaagg ctttattgta aaccaaagcg gtttgtcgga acgaagcggg tcggttttgc
     2581 ggtatcgcaa acctgttttc agacgacctt gcatattata ctcaagttga gcataatgta
     2641 aaaacagttt gaaaaacaga cggcaaacct tcatgccgtc tgaaaaccag ctacaaggaa
     2701 acatcatgca aaccgccgcc cgccgctcgt tcgattacga tatgcccctc atccaaacgc
     2761 cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg cccgacgacg
     2821 agacggcagg tcgtctgaaa gacgaaatca aggccttgct gaagcagaaa aacgcggtct
     2881 tggtggcgca ttattacgtc gatccgctga ttcaggattt ggctttggag acgggcggat
     2941 gcgtgggcga ttcgctggaa atggcgcgct tcggcgcgga acacgaagcc ggtacgctgg
     3001 tggtggcggg cgtgcgtttc atgggcgaga gcgcgaaaat cctctgccct gaaaaaacgg
     3061 tgctgatgcc tgatttggaa gcggaatgtt ctttggattt gggttgtccc gaagaagcgt
     3121 tttcagcgtt ttgcgaccaa caccccgacc gcacggtggc ggtgtacgcc aatacttccg
     3181 cagccgtgaa agcgcgtgcc gactgggtgg taacgtcttc ggtggcgttg gaaatcgtgt
     3241 cgtatctgaa atcgcgcggc gagaaactga tttgggggcc cgaccgccat ctcggcgact
     3301 acatccgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc atcgtccata
     3361 acgaattcaa agggcaggaa ctggcggcgt tgaaggcaga gcatcccgat gctgtggtgc
     3421 tggttcatcc cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc ggctcgacca
     3481 gcaaactact caaagccgcc gtatcgcgcc ctgaaaaaaa attcatcgtg gcaaccgatt
     3541 tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa agaatttatc gccgcgccga
     3601 cggcgggcaa cggcggaagc tgtaaaagct gcgcgttctg cccgtggatg gcgatgaatt
     3661 cgctgggcgg catcaaacac gccctgacag gcggacgcaa cgaaatcctg ttggacagaa
     3721 agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgacttcgcg gcaggactca
     3781 aacgcgggga tgtgttcaac ggcatggggc ccgcctgatt tgccgtccat atcgccgttt
     3841 cagacgacct ctcaaacaag gacaacacca tgcaaaccga ttgcgacgta ttgattgccg
     3901 gaaacgggct ggcggcactg acgctcgccc tgtcgctgcc tgaatcgttc cgcatcgtca
     3961 ttttgtgcaa aaaccggctg gacgacaccg ccagccgtca tgcgcaaggc gggattgcgg
     4021 cggcgtggtc gggagaggac gacatcggaa aacacgtcgc cgatactttg gaagcgggcg
     4081 caggtttgtg cgacgaagcc gccgtccgca ccatcctgtc gcagggcaaa ccggcaatcg
     4141 aatggctgct ggcgcagggc gtggcgttcg accggaatca taacgacctg cacctgacgc
     4201 gcgaaggcgg gcatacctgc cgccgaatcg cccacgtcgc cgactacacg ggcgaagccg
     4261 tcatgcagag cctgattatc caaatacgcc gccgaccgaa catccgcgtt tacgagcggc
     4321 agatggcgtt ggacgttcaa accgaatcag gcgcagcgtg cggactgacc gtcctcgact
     4381 gccgaacgca agaaacctac cgcatccgtg cccgccatac cgtactcgca ggcggcggcc
     4441 tcgggcaaat ttacgccgcc accaccacgc cgcccgaatg cacgggcgac gccatcgcca
     4501 tggcgatacg cgcgggttgc gcaatcgaaa acctcgaatt tatccaattc caccccacag
     4561 gcttggcaag gccgtctgaa aacggccgca ccttcctgat ttccgaagcc gtgcgcggcg
     4621 aaggcggcat cctgaccaat caatcgggcg aacggtttat gccgcattac gaccgccgcg
     4681 ccgaactcgc gccgcgcgac atcgttgccc gcgccatcgc cgccgaaacc gccaaacaaa
     4741 cgcaagactt cgtctcgctc gacatcagcc gccaacccgc agcgttcgtc cgtcggcatt
     4801 tcccgtccat ccatcggcac tgcctgtccc aatgcggctt ggacatcacg cgccaagcca
     4861 tccccgtccg ccccgtgcaa cactacacct gcggcggcat ccaaaccgac cccagcggca
     4921 gaacctccct gccgcagctc tacgccttag gcgaaaccgc ctgtaccgga ctgcacggag
     4981 ccaaccgcct cgccagcaac tccctgctcg aatgcgtcgt taccgccagg cttgccgccc
     5041 aaggcatcgc ggacggacaa gcgttccaaa tcgaaccatt ccaaaggccg tctgaaagcc
     5101 cctccgccga aacagacatc ttttcaaacg acctccaaaa cacattcagc cgccctgtcc
     5161 tgcaaacgtt caaccaacgc catctcggca tcctccgcaa cgataccgac ctgcaccgcg
     5221 ccatcgacca actgcggctt tggaagcaaa accaagccga accgcacacc gcgtccgaat
     5281 acgaaaaccg caacctactc gaatgcagcc tcgccgtcgc ccaagccgca tacaggcgga
     5341 gacagaacat cggtgcgcat tttaatagtg attgttaagg ttggctggtt agaaaaaaat
     5401 cgttcaaata aatttgaata gcatggttaa gagattggca ttcagttttt atggttgcgt
     5461 aagttggg
//
LOCUS       DAWTHD010000117         5441 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWTHD010000117 DAWTHD010000000
VERSION     DAWTHD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.117"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..361
                     /locus_tag="WM704_001889"
     CDS             <1..361
                     /locus_tag="WM704_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341238.1"
                     /translation="YPSGIMDISLVSFSTDLSNCILTIRTSTKPSVEIEKWGLWLKDY
                     DTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKIIRFYDNNSNWLLELEVY
                     GLVFQGCKTYMKEGYES"
     gene            351..1241
                     /gene="ubiA"
                     /locus_tag="WM704_001890"
     CDS             351..1241
                     /gene="ubiA"
                     /locus_tag="WM704_001890"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFA9341239.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            1426..1875
                     /gene="ptsN"
                     /locus_tag="WM704_001891"
     CDS             1426..1875
                     /gene="ptsN"
                     /locus_tag="WM704_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFA9341240.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            1879..2841
                     /locus_tag="WM704_001892"
     CDS             1879..2841
                     /locus_tag="WM704_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HFA9341241.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            2822..3676
                     /gene="rapZ"
                     /locus_tag="WM704_001893"
     CDS             2822..3676
                     /gene="rapZ"
                     /locus_tag="WM704_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFA9341242.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            3757..4698
                     /gene="ylqF"
                     /locus_tag="WM704_001894"
     CDS             3757..4698
                     /gene="ylqF"
                     /locus_tag="WM704_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFA9341243.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            4799..5401
                     /locus_tag="WM704_001895"
     CDS             4799..5401
                     /locus_tag="WM704_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFA9341244.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
BASE COUNT         1341 a         1417 c         1432 g         1251 t
ORIGIN      
        1 atatcctagt ggcataatgg atatttcact agtctctttt tcaactgact tgtctaattg
       61 tattttaact atccgaacaa gtacaaagcc ttctgtagaa atcgaaaaat gggggctgtg
      121 gctaaaagat tatgatacag ttgaaattga attaagaaat agctttatta aaggaatgaa
      181 atgtcaaaat tggtcgcata acaatagaaa tatatgccaa gtagaaataa agaaccaaga
      241 agatggtcta aaaataataa gattttacga caataattca aattggttat tggaactaga
      301 agtttatgga ttagttttcc aagggtgtaa gacttatatg aaagagggtt atgaatctta
      361 aatccccttt atttttacga ctgtccgatc gtttggacgt ttatatcaga ctgatgaggg
      421 cggacaagcc cattgggacg ctgcttttac tgtggccgac ctactgggca ttgtggctgg
      481 cttcagacgg cattcccgat ttggcggtat tggcggcgtt tacaatcggc acgtttttaa
      541 tgcgcagtgc cggctgcgtc atcaacgact ttgccgaccg cgattttgac ggtgctgtcg
      601 agcgcaccaa aaaccgtccg ttcgcacagg gcagggtcaa gaaaaaagaa gccctgctgc
      661 tgacggcatt tttgtgtctg cttgccgcat tgtgcctgat tccgctgaat catctgacat
      721 ggctgatgag cctgcccgcg ctgttccttg cgctgactta cccgtttacc aaacgctttt
      781 ttccgattcc ccaattttat ctgggacttg ccttttcctt cggcatcccg atggcgtttg
      841 ccgcagtggg caacagcgtt cccgttgaag cgtggatact ctttgccgcc aatgttttat
      901 ggacactggc ttacgatacc gtctatgcaa tggcggacaa agaggacgat ttgaaaatcg
      961 gcatcaaaac ctccgccgtc acgttcgggc gttacgacat cgccgccgtt atgctgtgtc
     1021 atggcggctt taccctgctg atggcagtat tgggtgcggt tatcggtgcg gcatgggcat
     1081 attggacggc aatccccatc gtcctgctgc tgcaataccg ccaatatgcc gccatcaaaa
     1141 gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa caacagaatc ggttgggtgt
     1201 ggtttgccgc tatttttgcc catacgtttt tcgcgaaata aggcagggca atgccgtctg
     1261 aagaaccgta aactgctttg gacggcattt ctatctgtgc cgaaaagcgt taaaatatgt
     1321 ttttgaaacg ctgtgttatg tcagcccgta ccgtatgcgg gattgaggtt tgccccggca
     1381 gtcggtacaa tccttctgtt ttgcgatgtc tgaaaagaga agcttatgag ccttatcggc
     1441 gaaattttgc ctttgtccca tattgttttg gatatggagg tgggcagtaa aaaaaggctg
     1501 tttgaggaag cgggcctgct tttggaacgc gaatcctcat tgtcccatgc tgatgttttc
     1561 gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt tggggcaggg tgtcgccatc
     1621 ccccacggcc gccacgcgag cgtgaagcag gcgacgggcg cgttcatccg tacgcgcgaa
     1681 cccgtcggat tcgacgcgcc tgacggcaaa ccggtttcac tggtttttat cttgctggtg
     1741 ccggaaaacg caacgggcga gcatttggaa gtcttatcca aactggccgg caagttttcc
     1801 caaaaaagca tcagagaatc gctgatgacg gttgcttctg cggaagaagc ccgcgccatc
     1861 ctgaccgaag aataaaatat gcccagtatc tccgtccgcc gcctgtttga cgacaaccaa
     1921 tacaagttgc aacttgcctg ggccgccggc aattcgggtg cggacaaccg tatcggcgta
     1981 gaggcggaca agcccgtcct cgccctggtc ggacacctga atttcatcca tcccaaccaa
     2041 atccaagtgg tcggtttggc ggagtcggaa tatctgaacc gcctcgaatc gggggagacg
     2101 ggttatcagt tcggagacct gttcgatatt tccatgtctt tggtcattgt ggcaaacgat
     2161 ttgccggttt ccccggggct gcgcgactac tgccacaaaa acgacatccc cctgttgact
     2221 tccaaactcg aaagccctta tctgatggat gtgttgcgga tttacctgca acgcaccttg
     2281 gcagcatcgt ccgtcaaaca cggcgtattt ctcgatgtgt ttgaaatcgg cgtgctgatt
     2341 accggacatt ctggtttggg taagagcgaa ttggcattgg aactgatttc gcgcggccat
     2401 agcctgattg ccgacgatgc ggtcgagctg ttccgcatcg gtccggaaac attggaaggg
     2461 cgttgttcgc ctatgctgcg ggattttttg gaagtgcgcg gcttggggat actcaatatc
     2521 cgccatattt tcggcgaaac ttccatccgc cccaaaaaaa tcctgcaact cattatcaat
     2581 ttggtcgagg cggacgacga gtatatgaag cagctcgacc ggttgagcat ccgcaccgaa
     2641 accgaatcca tcctcaacgt caacgtccgt tcggttacgc tgcccgtcgc cgtcggacgc
     2701 aaccttgccg ttttggttga ggcggcggta cgcaattaca ttttgcagtt gcgcggcaaa
     2761 gacagtacgc gcgaattttt ggaacgccat cagacgcaac ttaaagaaaa cgaacagaat
     2821 catgaaaatc gtcctgatta gcgggctttc cggctcgggc aaatccgttg cactgcgcca
     2881 aatggaagat ttgggctatt tttgcgtgga caacctgccc ttggaaatgc tgccgtcgct
     2941 ggtgtcgtac catatcgaac gtgcggacga aaccgaattg gcggtcagcg tcgatgtgcg
     3001 ttccggcata gacattgccc aagcgcgcga gcagattgcc tatctgcgcg gactcggaca
     3061 cagggtggaa gttttgtttg tcgaggcgga agaggccgtg ctggtccgcc gcttttccga
     3121 aacccggcgc ggacatcctt tgagcaatca ggatatgacg ctgttggaaa gcctgaaaaa
     3181 agagcgcgaa tggctgttcc cgcttaagga aatcgcctac tgtatcgaca cttccaagat
     3241 gaatgcccaa cagctccgcc atgcagtccg gcagtggctg aaggttgagc gtaccggact
     3301 gctggtggtt ttggagtcct tcgggttcaa atacggtgtg ccgaacaacg ccgattttat
     3361 gttcgatatg cgcagtctgc ccaacccgta ttacgatccc gagttgaggc cttacaccgg
     3421 tatggacaag cccgtttggg attatttgga cggacagccg ttggcgcagg aaatggttga
     3481 cggcatcgaa aggtttgtta cgcgttggtt gccgcgtttg gaggatgaaa gcaggagcta
     3541 cgttaccgtc gccatcggct gtaccggcgg gcagcatcgt tcggtctata ttgtcgaaaa
     3601 actcgcccga aggttgaaag ggcgttatga attgctgata cggcacaggc aggcgcaaaa
     3661 cctgtcgggc cgctaatccc gtcaaaccat tatgccgtct gaaaccccgt ccgccgtttc
     3721 agacggcatt tcaaatattg agaagaaaga acagacatgg caatccaatg gtttcccggc
     3781 catatgaaca aggcaagaaa agctatcgcc gagcgtgcca aaagcgttga tatggtgatt
     3841 gaaatgctgg acgcgcgtat gcccgcctcc agcgaaaatc cactgttggc gcagctttcc
     3901 aaaggtaagc ccaaactcaa aattttaaac aagcaagacc ttgccgaccc tgaacgcaca
     3961 aaggtctggc ttgaccacta caacagccgc cccgacaccc gggccatcgc cctcgattcc
     4021 tccgaaaccg gcgcacacgg caaaatcacc caagcctgcc gcgcgatgat tccccaccgg
     4081 caaggcatag acaaacccct gcgcgttttg atttgcggca ttcccaacgt cggcaaatcc
     4141 accctcatca atggaatgat aggcaaaaaa tccgccaaaa ccggcaacga acccggcatt
     4201 accaaagccg aacaacgcct tttccttgcc gacgacttct ggctgtacga caccccggga
     4261 atgctgtggc cgaaaatcat cgtcgaagaa agcggctaca acctcgccgc cggcggcgca
     4321 gtcggacgca acgcattgga cgaagaagaa gtcgccctcg aacttttaga ctacctccgc
     4381 cgccactacc tccctatgct gcaagaacgc taccaagccg acaaagaccc cagcagccac
     4441 tgggacgaca actcgtggct cgaatggata gccaaaaaac gcggcgcagt cctcagcggc
     4501 ggacgggtca actaccaaaa agccgccgaa aacatcctca cggacttccg cgaaggcaaa
     4561 atcggcagaa tcacccttga aacaccgaac caatgggaaa cttggcttaa aaaagcacgt
     4621 cagaaagaag ccgaactcaa agccattcgc gaagccagaa aagcagagcg caaagggcag
     4681 aagccttcgg aagcataaag aatgccgtct gaaaaatatt tttcaggcag cttctctcta
     4741 ctccaaccga tttcagacgg catatccaaa cccatgccgt ttcagcacgg atacccgtat
     4801 gaccgacaaa atttctcccg acgcgctgat tgaagccgca ctgctgaccc aaaccgaacc
     4861 gctgaccgaa aaatctatgc gcgaactgtg tgtgccgccg ttgtcgcaag acaaactgat
     4921 tgatgtgttg gcgcagttga aaacgcgttg gcaggatagg gcgttgcaac tggtgcatac
     4981 gcaggagggc tggcgttttc agattgttca gacggcattc gagcggctgg gcagcctgca
     5041 agaacagcgt gcgccgcgct actcccgcgc cgtgatggaa acactggcga ttatcgccta
     5101 ccagcagccc gtaacgcgcg gcgacatcga aggtatacgc ggcgtggcgg tgtcgcagaa
     5161 cgtgatgcag accttgcagg atcgggggtg gattgaagtc atcggacatc gggacacatt
     5221 gggaaaaccc gcattgtggg caacaacggc aacttttctc agcgatttgc gcttggacgg
     5281 tttggaagaa ctgccgccgc tgaccgaact gggcgaactg gttttgcccg atttgataga
     5341 aatgccgcct acggatgaag aagagccgga aaccgtaccg tccgataccc tgcccaactg
     5401 aaattccaaa tgccgtctga aacgcacatt gcttcagacg g
//
LOCUS       DAWTHD010000118         5184 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWTHD010000118 DAWTHD010000000
VERSION     DAWTHD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.118"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            125..2248
                     /locus_tag="WM704_001896"
     CDS             125..2248
                     /locus_tag="WM704_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9341245.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     HDVPSKKPEARKDQGGYGFAMRFKRRNRHPMAMPKENEVKLKDDDWEATGLPGDPKDL
                     PGRQKSVIDEVSANGNNDIYFSPYLKPSNHQNSSINGSANQPRNEVKDYKNFEYVYSG
                     WFYKHAKPIIDGTQNKLQQGDDGYIFYHGKDPSRQLPASEKVIYKGVWHFVTDTKRGQ
                     KFNDILETSKKQGDSYSGFSGDEGETTSNRTDSNLNDKHEGYGFTSNFEVDFNNKKLT
                     GKLIRNNKVTDAAASNGYTTEYYTLDATLRGNRFSGKATATDKSSNEQAKLHPFVSDS
                     SSLSGGFFGPQGEELGFRFLSDDNKVAVVGSAKTKDKNANGNTAAAGTAGAAGMSSED
                     TKLTTVLDAVELKSDGKKVENLDNFSDATRLVVDGIMIPLLPTESGNGQADKGKNGKT
                     AFIYETTYTPESDKKDTKAGTAANGVQTVSNTAGGTSGKTKTYKVQVCCSNLNYLKYG
                     LLTRENNNSVMQAVKNSNRTADRTAQGAQSMFLQGERTDEKEIPKDENVVYLGTWYGH
                     IAANGTSWTGNASDQQSGNRARFDVNFKDKKITGTLTAANRQAETFTISGMIDGNGFE
                     GTAKTGNDGFAPDQNSSTGTYKVHIANAEVQGGFYGPNAEELGGWFAYPGNEQTKNAQ
                     ASSGTGNSAGSATVVFGAKRQELVK"
     gene            2335..5079
                     /gene="tbpA"
                     /locus_tag="WM704_001897"
     CDS             2335..5079
                     /gene="tbpA"
                     /locus_tag="WM704_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFA9341246.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVALTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDDASTYAHFIVEEECKNGGYEKCKANP
                     KKDVAGEDKRQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKYGSIRGYGKYAGDHRYGGLITNSENGAQVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSD
                     LRHQDYYYQHANRAYSPKTLPQNGDKKTIPNGGKDKPYWVSIGGGNVVTGQICLFGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEVQIKDGKEGVKGDPAYLNAQSARITGINILGK
                     IDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTVK
                     KHFTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
BASE COUNT         1490 a         1335 c         1321 g         1038 t
ORIGIN      
        1 aaaagcagcg cgggaatgac ggtattttgg tttatttact aaaaatataa aaaagcattt
       61 gcaaattttt taaaaataaa taaaataata atccttatca ttctttaatt gaatcgggtt
      121 tgttatgaac aatccattgg tgaatcaggc tgctatggtg ctgcccgtgt ttttgttgag
      181 cgcttgtctg ggcggaggcg gcagtttcga tcttgattct gtcgataccg aagccccgcg
      241 tcccgcgcca aagtatcacg atgttccttc caaaaaaccg gaagcccgaa aagaccaagg
      301 cggatacggt tttgcaatgc gcttcaagcg gcggaatcgg catcctatgg caatgcctaa
      361 agaaaatgag gttaaattaa aggatgatga ttgggaggcg acaggattgc caggcgatcc
      421 caaagactta cctgggcgac agaaatcggt tattgacgaa gtgtcagcca acggcaacaa
      481 cgatatttat ttctctccct atctcaaacc atcaaaccat caaaacagca gcattaatgg
      541 cagtgcaaac caaccaagaa atgaagtaaa agattataaa aatttcgaat atgtttattc
      601 cggatggttt tacaaacacg ctaaaccaat catagatggg actcaaaaca aattgcaaca
      661 aggcgacgac ggttatatct tttatcacgg taaagaccct tcccgacaac ttcccgcttc
      721 tgaaaaagtt atctacaaag gcgtatggca ttttgtaacc gatacgaaac ggggacaaaa
      781 atttaacgat attcttgaaa cctcaaaaaa gcaaggcgac agctacagcg gattttcggg
      841 cgatgaaggc gaaacaactt ccaatagaac tgattccaac cttaatgata agcacgaggg
      901 ttatggtttt acctcaaatt ttgaagtgga tttcaataat aaaaaattga cgggcaaact
      961 gattcgcaac aataaagtta cagacgctgc tgctagcaac ggatatacca ccgaatacta
     1021 cacccttgat gcgacgctta ggggcaaccg cttcagcggc aaggcgacgg cgaccgataa
     1081 atccagcaat gaacaagcca aactacatcc ctttgtttcc gactcgtctt ctttgagcgg
     1141 cggctttttc ggcccgcagg gtgaggaatt gggtttccgc tttttgagcg acgacaacaa
     1201 agttgccgtt gtcggcagcg cgaaaaccaa agacaaaaac gcaaatggca atactgcggc
     1261 ggctggcacc gcaggtgcgg caggcatgtc gtctgaagac actaagctga ccacggtttt
     1321 ggatgcggtc gagctgaaat cggacggtaa gaaagtcgaa aatctcgaca acttcagcga
     1381 cgctacccga ctggttgtcg acggcattat gattccgctc ctgcccaccg aaagcgggaa
     1441 cggtcaggca gataaaggta aaaacggcaa aacagccttt atctacgaaa caacctacac
     1501 gccggaaagt gataaaaaag acaccaaagc aggtacggcg gcgaatggcg tgcaaaccgt
     1561 ttcaaatacg gcaggcggca caagtggcaa aacaaaaacc tataaagtcc aagtctgctg
     1621 ttccaacctc aattatctga aatacgggct gctgacacgt gaaaacaaca attccgtgat
     1681 gcaggcagtc aaaaacagta accgaactgc cgaccgaacg gcacagggcg cacaaagtat
     1741 gttcctccaa ggcgagcgca ccgatgaaaa agaaattcca aaagacgaaa acgtcgttta
     1801 tctaggcact tggtacgggc atattgccgc caacggcaca agctggaccg gcaacgcctc
     1861 cgatcagcaa agtggtaatc gggcaaggtt tgacgtgaat ttcaaagaca aaaaaattac
     1921 cggcacgtta accgctgcaa acaggcaggc ggaaaccttt accattagtg gaatgattga
     1981 tggcaacgga tttgaaggta cggcgaaaac cggtaatgac ggatttgcgc cggatcaaaa
     2041 cagtagcacc ggtacatata aagtgcacat cgcaaatgcc gaagtgcagg gcggttttta
     2101 cgggcctaac gccgaagagt tgggcggttg gtttgcctat ccgggcaatg aacaaacgaa
     2161 aaatgcgcaa gcttcatccg gcactggaaa ttcagcaggc agcgcgaccg tggtattcgg
     2221 tgcgaaacgc caagagcttg tgaaataagc acggctgccg aacaatcaag aataaggctt
     2281 cagacggcat cgttcctgcc gattccgtct gaaagcgaag ggtagggaaa cactatgcaa
     2341 cagcaacatt tgttccgatt caatatttta tgcctgtctt taatgactgc gctgcccgct
     2401 tatgcagaaa atgtgcaagc cggacaagca caggaaaaac agttggatac catacaggta
     2461 aaagccaaaa aacagaaaac ccgccgcgat aacgaagtaa ccggtttggg caaattggtc
     2521 aaaaccgccg acaccctcag caaggaacag gtactcgaca tccgagacct gacgcgttac
     2581 gaccccggca tcgccgtcgt cgaacagggg cgcggcgcaa gctcgggcta ctcgatacgc
     2641 ggtatggaca aaaaccgcgt cgccttgacg gtggacggct tggcgcaaat acagtcctac
     2701 accgcgcagg cggcattggg cgggacgagg acggcgggca gcagcggcgc aatcaatgaa
     2761 atcgagtatg agaacgtcaa ggctgtcgaa atcagcaaag gctcaaactc ggtcgaacaa
     2821 ggcagcggtg cattggcggg ctcggtcgca tttcaaacca aaaccgcaga cgatgttatc
     2881 ggggaaggca ggcagtgggg cattcagagt aaaaccgcct attccggcaa aaaccggggg
     2941 cttacccaat ccatcgcgct ggcggggcgc atcggcggtg cggaggcttt gctgatccgc
     3001 accgggcggc acgcggggga aatccgcgcc cacgaagccg ccggacgcgg cgttcagagc
     3061 tttaacaggc tggcgccggt tgatgacgcg agcacatacg cccatttcat cgttgaagaa
     3121 gaatgcaaaa acgggggtta cgaaaagtgt aaagcgaatc cgaaaaaaga tgttgccggc
     3181 gaagacaaac gtcaaacggt ttccacccga gactacacgg gccccaaccg cttcctcgcc
     3241 gatccgcttt catacgaaag ccggtcgtgg ctgttccgcc cgggttttcg ttttgagaat
     3301 aagcggcact acatcggcgg catactcgaa cgcacgcaac aaactttcga cacgcgcgat
     3361 atgacggttc cggcattcct gaccaaggcg gtttttgatg aaaataaaaa atacggatcg
     3421 ataagaggct acggcaaata cgcgggcgac caccgatacg gcgggctgat taccaacagt
     3481 gaaaacggtg cgcaggtggg cgcggaatac ggtacgggcg tgttttacga cgagacgcac
     3541 accaaaagcc gctacggttt ggaatatgtc tataccaatg ccgataaaga cacttgggcg
     3601 gattatgccc gcctctctta cgaccgtcag ggtatcggtt tggacaatca ttttcagcag
     3661 acgcactgtt ccgccgacgg ttcggacaaa tattgccgcc cgagtgccga caagccgtct
     3721 tcctactaca aatccgaccg cgtgatttac ggggaaagcc acaggctctt gcaggcggca
     3781 ttcaaaaaat ccttcgatac cgccaaaatc cgccacaacc tgagcgtcaa cctcggttac
     3841 gaccgttttg gttccgacct ccgccatcag gattattatt atcaacatgc caaccgcgcc
     3901 tattcgccga aaacgctccc tcaaaacggc gataaaaaaa ccatccccaa cggcggcaag
     3961 gacaagccct attgggtcag cataggcggg ggaaatgtgg ttacggggca aatctgcctc
     4021 tttggcaaca atacttatac ggactgcacg ccgcgcagca tcaacggcaa aagctattac
     4081 gcggcggtcc gggacaacgt ccgtttgggc aggtgggcgg atgtcggcgc gggcttgcgc
     4141 tacgactacc gcagcacgca ttcggacgac ggcagcgttt ccaccggcac gcaccgcacc
     4201 ctgtcctgga acgccggcat cgtcctcaaa cctgccgact ggctggattt gacttaccgc
     4261 acctcaaccg gcttccgcct gccctcgttt gcggaaatgt acggctggcg gtcgggcgat
     4321 aaaataaaag ccgtcaaaat cgatccggaa aaatcgttca acaaagaagc cggcatcgtg
     4381 tttaaaggcg atttcggcaa cttggaggca agttggttca acaatgccta ccgcgatttg
     4441 attgtccggg gttatgaagt gcaaattaaa gacggcaaag aaggagtcaa aggcgacccg
     4501 gcttacctca atgcccaaag cgcgcggatt accggcatca atattttggg caaaatcgat
     4561 tggaacggcg tatgggataa attgcccgaa ggttggtatt ccacatttgc ctataatcgt
     4621 gtccgtgtcc gcgacatcaa aaaacgcgca gaccgcaccg atatccaatc acacctgttt
     4681 gatgccatcc aaccctcgcg ctatgtcgtc ggctcgggct atgaccaacc ggaaggcaaa
     4741 tggggtgtga acggtatgct gacttattcc aaagccaagg aaattacaga gttgttgggc
     4801 agccgggctt tgctcaacgg caacagccgc aatacaaaag ccaccgcgcg ccgtacccgc
     4861 ccttggtata ttgtggatgt gtccggttat tacacggtta aaaaacactt caccctccgt
     4921 gcgggcgtgt acaacctcct caaccaccgc tatgttactt gggaaaatgt gcggcaaact
     4981 gccgccggcg cagtcaacca acacaaaaat gtcggcgttt acaaccgata tgccgccccc
     5041 ggccgcaact acacatttag cttggaaatg aagttctaaa cgtccgaacg ccgcaaatgc
     5101 cgtctgaaag gcttcagacg gcgttttttt acacaaattt caccgttccc catccctccc
     5161 gatacaccgt aatcccgaaa cccg
//
LOCUS       DAWTHD010000119         5170 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWTHD010000119 DAWTHD010000000
VERSION     DAWTHD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.119"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            97..918
                     /locus_tag="WM704_001898"
     CDS             97..918
                     /locus_tag="WM704_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HFA9341247.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1004..1666)
                     /locus_tag="WM704_001899"
     CDS             complement(1004..1666)
                     /locus_tag="WM704_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9341248.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1704..2531)
                     /locus_tag="WM704_001900"
     CDS             complement(1704..2531)
                     /locus_tag="WM704_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFA9341249.1"
                     /translation="MKPDIYALPERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2914..3537
                     /gene="lolA"
                     /locus_tag="WM704_001901"
     CDS             2914..3537
                     /gene="lolA"
                     /locus_tag="WM704_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFA9341250.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3810..4949
                     /locus_tag="WM704_001902"
     CDS             3810..4949
                     /locus_tag="WM704_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFA9341251.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5044..>5170
                     /locus_tag="WM704_001903"
     CDS             5044..>5170
                     /locus_tag="WM704_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9341252.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSK"
BASE COUNT         1194 a         1413 c         1440 g         1123 t
ORIGIN      
        1 ggggtgtaat ccgaggtagg ggcggctggg gtgcttctcc cttgtctgcc gctgctgtta
       61 tgatgatatt tttattctgt atttaaggag ggggtgatga gcagtccgcg ccaagtgttt
      121 tatatttccg accgcaccgg tctgactgcc gagaatatcg gcgaggcttt gctgaaccag
      181 tttggcaatc tgtcgttcaa acgccatacg catccgtttg tcgatacgcc ggaaaaggcg
      241 cgcgcggtgg tggagaaggt caatcggagc cggcaggaaa acggtcagcg tccgattgcg
      301 tttgtcagtg tggttgatga cgaaatccgc cggattatta aaggggcgga tgcttttcag
      361 attaatttct ttgagacttt tttgggactg ttggagaagg aactcaatac cgaagcaacg
      421 gtatccgggc aggggcatca cagtatcggc aatacgaagc gttacgatgc gcgtatggag
      481 gcagtcaatt tttctttgaa ccatgatgac ggggtcagcg ataagaacct tcaggaggcg
      541 gatgtgattc tgatgggcgt gtcccgttcg ggcaaaacgc cgacctgcct gtatctggcg
      601 ttgcaatacg gtatccgtgc ggcaaactat ccgctgattc ccgatgattt ggaatcggcc
      661 gatctgccgc gtatggtcaa gccgtataaa gacaaactgt tcgggttgac tatccagccg
      721 gaacgtttgc aggccatccg tcaggaacgc cgcccgaatt cggcttatgc gcgcatcgac
      781 acctgccgca gcgaagtggc ggacgcgcag agtatgttca gacggcatgg cattccgttt
      841 gcgaatacga cggacaagtc ggttgaggaa ctggcggtac acatccttca ggcgtgcaag
      901 ctcaaacgca ggttttgacg ggctttgatt cggtttgaag gtggaaatgc cgtctgaaat
      961 caggtttcag acggcagttt tatgtttgtg gggcggatat ttttcagggc tgtattttgt
     1021 ccagacattc gagcagatcg agcggcgtgc ggatgtggaa atccgcctgc catgagccgg
     1081 tatcgtcttc atcggaaata tagccccatt cggcgaggac ggttttcata ccggcgttgc
     1141 gtccggcttg gatgtcgcgt tccgcgtcgc cgacgtagag ggtgtgttgc gggtcggcgt
     1201 ggatttttcc gcaggcgtgc agcatgggtt tgatgctggg tttgggttcg ccgcaggtgt
     1261 cgccgctgac gacggtggcg ggcggaacgg cgaatccgag tttgggaacg agtttgtcgg
     1321 tgaagcgcat gggtttgttg gtgatgatgc cccatttgat gccgcgtctg ccgagttcgg
     1381 cgatgagttc gttcacgccg tcgaagagag tggtgtcttg ggcgtagcgg ctgtcgtatt
     1441 cctccagata ttcggtgcgc catgcggtat agtcggggtg ttcgggcgtg atgttcgcgc
     1501 cgagtttgag caatcctgcc gcgccgtggc tggcttgggt gcggatttcg tccatgcttt
     1561 tttcgggcag tccgtggcgg gcgagttggg tgttgagtgc gccgccgagg tctagggcgg
     1621 tgtcggcgag cgtgccgtcg aggtcgaaca atacggcttg tatcatgtgt tttccttttt
     1681 tataaagtgt gggacgaaag gtttcagacg gcatgtttat tttgtttcaa accctgctcg
     1741 aaatcttcca acatatccaa ttcgaagcgg ctgaagcccg ctttttcccg cgcttcgatg
     1801 ttcacatagc cgcggaagat aaacatatcg taacgggcaa tcagactgcg gaacagggcg
     1861 acaggttcca aaccgcgttc gcggcagagg tgttgatacc accggttgcc gatggcgacg
     1921 tgtcccactt cgtcgcggta aatgatgtcc aacacgccgc aggttgccga atcgccgcgc
     1981 tgctccacct tcgcgcgtat ccccggcgta acgtccagtc cgcgcgcctc caacacgcgc
     2041 ggtaccaacg ccatacgcaa cagcgggtcg taggcggttt tgtacgccat atcccacaaa
     2101 tggttgtgcg cctcgaaact gccgtaatcg aagccgaaag cgcgcaatct gtcgcgcacc
     2161 aggcggaagt ggtacacctc ctccttcgcc actttcaccc agtcgcggac aaactgaaac
     2221 ggcagcgtgc ggaaacggta tgccgcgtcc aaagccagat tgacggcgtt gaactcgata
     2281 tgcgtaatcg cgtgcagcat cgccgcatag ccttctgccg tgttcatttt gcgcggggtc
     2341 agttgcgagg gcgcgaccaa aacaggcgtg tccggccgtc ccgcgcgggg gaagtccgcc
     2401 ggcggcgcgt ttgcctccgc cccgtccgca ttttgaacgg cggcaaacgc ctcatccgtc
     2461 agccgtcctt tttcatcggg gtcgcccgaa agcagggcgc gttccggcaa agcataaata
     2521 tcgggtttca tctcaagtcc gccgtgttcg gaaaacgaat attatagcgt ttaaaaaaaa
     2581 caagatgagg catataatct ccgcgattcg gcattccgcg cccaaaccgt caaatatagt
     2641 ggattaacaa aaaccagtac ggcgttgccc cgccttgccg tactatgttg tactgtctgc
     2701 ggcttcgtcg ccttgtcctg atttttgtta atccactata acgcggcaca caattgaaag
     2761 ggcagcgtgg cgcgcctgct ttttccgagc ggtcaaaaaa atcagccctc ggaaaacgcg
     2821 gtttgcaaaa tgcaaaccgc ccgtaacgcc gcccgtatga ttgttttgcc gcgccgatac
     2881 tttgcgccac acccatcccg acaaggaaaa ataatgatga aaccgcacaa cctgttccaa
     2941 ttcctcgccg tttgctccct gaccgtcgcc gtcgcttccg cacaggcggg cgcggtggac
     3001 gcgctcaagc aattcaacaa cgatgccgac ggtatcagcg gcagctttac ccaaaccgtc
     3061 caaagcaaaa agaaaaccca aaccgcgcac ggcacgttca aaatcctgcg cccgggcctc
     3121 ttcaaatggg aatacacttt gccctacaaa cagactattg tcggcgacgg tcaaaccgtt
     3181 tggctctacg atgttgattt ggcacaagtg accaagtcgt cccaagacca ggccatcggc
     3241 ggcagccccg ccgccatcct gtcgaacaaa accgccctcg aaagcagtta cacgctgaaa
     3301 gaggacggtt cgtccaacgg catcgattat gtgcgggcaa cgcccaaacg caacaacgcc
     3361 ggctaccaat acatccgcat cggcttcaaa ggcggcaacc tcgccgccat gcagcttaaa
     3421 gacagcttcg gcaaccaaac ctccatcagt ttcggcggtt tgaataccaa tccccaactc
     3481 tcgcgcggcg cgttcaagtt taccccgccc aaaggcgtgg acgtgttgag caactgatgc
     3541 cggccgcccc gatgccgtct gaacgccgcc gaggcttcag acggcatttt cacgcaggcg
     3601 gaacaatgtc ccgcattacc gcccgatcgg gcaacggaac ggcaaatccg tgaaaattaa
     3661 cggttgcgcc ccggctgttt ttgccgttta atgcaaacct tgctgcacca agggccaaga
     3721 aagccgaccg gccgccccca cagcttccga tgcaggcggc ccgtccgtcc ctgcaatgtt
     3781 ttttattttt gaacgaaagg tcgaaaacca tgaaaaaaac actggtggcg gcggcaatcc
     3841 tgagccttgc cttgactgcg tgcggcggcg gaagcgatac cgccgcccaa accccctccg
     3901 ccaagcccga agccgaacaa tcgggcaaac tcaacatcta caactggtcg gattatgtcg
     3961 atcccgaaac cgtcgccgcc tttgaaaaag aaaccggcat taagatgcgt tccgactatt
     4021 acgacagcaa cgaaacactg gaggcaaaag tcctgaccgg caaatccggc tacgacctga
     4081 ccgcgccgtc catcgccaac gtcggccggc aaatcaaagc gggcgcgtat cagaaaatcg
     4141 acaaggcgca aatcccccat tacggcaaca tcgataaaga tttgctgaaa atgatggaag
     4201 ccgtcgatcc gggcaacgaa tacgccgtcc cctatttctg gggcatcaac accttggcaa
     4261 tcaacacgcg gcaggtgcaa aaggcattgg gtacggacaa gctgcccgaa aacgagtggg
     4321 atttggtgtt caaacccgaa tacaccgcca aactcaaatc ctgcggcatc agctatttcg
     4381 acagcgcaat cgaacagatt cccttggcgt tgcactattt gggcaaagac cccaacagtg
     4441 aaaatcccga agacatcaaa gccgccgtcg atatgatgaa agccgtccga ggcgacgtga
     4501 aacgcttcag ctcttccggc tatatcgacg atatggcggc gggcaacctg tgtgccgcca
     4561 tcggttacgg cggcgatttg aacattgcca aaacccgtgc cgaagaagcc gcaaacggcg
     4621 tggaaatcaa agtattgacc ccgaaaaccg gcgtaggcgt gtgggtggat tcctttatga
     4681 ttccgcgcga cgcgcaaaac gttgccaacg cccaccgcta tatcgactac acgctccggc
     4741 ccgaggtggc tgcgaaaaac ggcagcttcg ttacctacgc gcccgccagc cgtcccgcgc
     4801 gcgagctgat ggatgaaaaa tacacctccg acgcatcgat tttcccgacc aaagaactga
     4861 tggaaaaaag tttcatcgta tcacccaaat ccgcagaatc cgtcaaactg ggcgtgaagc
     4921 tgtggcaagg gctcaaagcg ggcaaataac cggaatccct gccgtctgaa acctttcggg
     4981 cggcaggaaa cggcgtgtcc gcattaagtc aggattagga ttatttagaa agatgagatg
     5041 gatatgaatt taagtattgt cgttcctatt tataatgtcg aaagttattt ggaagcgtgt
     5101 ttaaattcta tagaacctat attaagtaat gaaaatgtcg aacttattct tgtgaatgac
     5161 gggtcaaaag
//
LOCUS       DAWTHD010000120         5040 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWTHD010000120 DAWTHD010000000
VERSION     DAWTHD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.120"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..642)
                     /locus_tag="WM704_001904"
     CDS             complement(<1..642)
                     /locus_tag="WM704_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9341253.1"
                     /translation="MQPAKNLLFSSLLFSSLLFSSLLFSSAAQAASEDGGRGPYVQAD
                     LAYAYEHITHDYPKPTAPNKNKISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYA
                     RYRKWNNNKYSVNTKEVLRNGNENSGGKLNIQTRKTEHQENGTFHAVSSLGLSAVYDF
                     KLNDKFKPYIGARVAYGHVRHQVRSVQQETDIVTTYPQNAKPSVTTGGPIPKSA"
     gene            1054..1974
                     /gene="ribF"
                     /locus_tag="WM704_001905"
     CDS             1054..1974
                     /gene="ribF"
                     /locus_tag="WM704_001905"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFA9341254.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            2116..4905
                     /gene="ileS"
                     /locus_tag="WM704_001906"
     CDS             2116..4905
                     /gene="ileS"
                     /locus_tag="WM704_001906"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFA9341255.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVPQA
                     EMVEIDRYALVLARQLQERVAGDFYPRYAFHFAVKEMVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKAGDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1216 a         1356 c         1363 g         1105 t
ORIGIN      
        1 ggcagatttg gggatcggac cacctgtggt aacacttggc ttagcattct gtgggtaagt
       61 ggtaacaata tcagtttctt gttgaaccga acgaacctga tgtctgacgt gtccgtaggc
      121 gacgcgcgcg ccgatatagg gtttgaattt atcgttgagt ttgaaatcgt aaacggcgga
      181 caagccgaga gaagaaacgg cgtggaatgt gccgttttcc tgatgttccg tctttcgggt
      241 ttgtatgtta agtttgccgc cagaattttc attaccgttt cttagcacct cttttgtgtt
      301 gacggaatat ttattgttgt tccactttct gtaacgggca taatctgccg ctatcctcca
      361 gccgccgaag tcgtagccga ccgacaccct ggggtggatg gaatgcgtac ggatgtttct
      421 gaaataatcg cttaccgtgc ttattttgtt cttgtttgga gcggttggtt tcggataatc
      481 gtgggtaatg tgttcgtagg cgtaggctaa atccgcctgc acatacgggc cgcggccgcc
      541 gtcttcactt gccgcctgag ctgcggaaga gaagagaaga gaagagaaga gaagagaaga
      601 gaagagaaga gaagagaaga gaaggttttt tgcgggctgg attcattttc ggctccttat
      661 atcggtttga ccggttaaaa aaagattttc actgatgttg aagggcggat tatatcgggt
      721 tccgggcggt gtttcaatac atagcaccgc gcctgctgcg cgttttatgc gtttggcgcg
      781 ttcggcggcg ggaaatttgc ctacttttcc cgcgtcgggc gggcggaacg ggcggcacac
      841 tgtctataaa ccgcaatacc gtttacaatg accgcctgtt tcaccacata cccgaacgca
      901 acaatgtttc aacacacggg acggcacatc aagcaccgcc ctatgtgtcg tcctgatttg
      961 gaaggggttg cacccctccc gaataaagtc tgatcctgcc gccccgaagg acggatgtcc
     1021 gagtggcggg gtttcaaccg aaaaggaaat acaatgaaaa tcaggccggg gcggcacaac
     1081 gcgcccgact ttccgcacgg ggccgccgta accataggca atttcgacgg cgtgcacctc
     1141 ggacacaaac acatcctcca aaaactccgc ctcgaagccg acgcacgcgg attgcccgtc
     1201 gttgccgtca ttttcgagcc gcaacccaaa gaattttttg ccgcccgcac aggcaaaacc
     1261 ccgccgtgcc gtatcagccc cctgcgtacc aaactcgggc tgctcgaaaa aaccggttgc
     1321 gtcgatgccg cctgggtttt gcgtttcgat cggaattttt ccgaaatatc cgcgcaagca
     1381 tttatcgacc gcctgctgcg tcaaaccttg aatacgcgct atttgctcgt cggcgatgat
     1441 ttccgtttcg gtgcggggcg ggaaggctgt tttgaacttt tggcacaaca gcccgatatg
     1501 caaaccgagc gcacgccttc cgtcatcgtc gaagacatcc gcaccagcag taccgccgtc
     1561 cgccaagccc tttcagacgg caaccttgcc tatgcgaaga aacttttggg gcacgactac
     1621 gttttggggg gcagggtggt gcacggcaga aaactcggac gcaccttaaa cgccccgacc
     1681 gccaatatcc gcctgcccgg ccaccgttat gcactcggcg gcgtgttcgt cgtcgaggcg
     1741 gacggcgcat tcggcacgcg gcgcggcgtg gcgagcttcg gcttcaatcc caccgttgat
     1801 ggcggctgtt ctcaaaagct tgaagtccac ctgttcgact ttcaaggcga tttgtacgga
     1861 caacggttga acgtccgctt cctgcacaaa ctgcgcgacg aggaaaagtt tgacggtatg
     1921 gaagaactga aaaggcggat tgaagccgat atggaagccg caaagtgttg gtagaaaaac
     1981 cttatacaaa ccatccgatt gggctacaat cagcctttta actgttcaga cggcacaggg
     2041 gtttcccgtt gtgaaatact gtttgagggg caatgccgtc tgaaaccgaa atattgtaac
     2101 aatagagatt aaaaaatgac cgattacagt aaaaccgtca acctgctcga aagcccgttt
     2161 ccgatgcgcg gcaatcttgc caagcgcgag cctgcgtggc tgaaaagctg gtacgagcaa
     2221 aaacgttacc aaaaactgcg cgaaatcgcc aaaggccgtc cgaaattcat cctgcacgac
     2281 ggcccgccgt atgccaacgg cgacatccat atcggtcatg ccgtcaataa aattcttaaa
     2341 gacattatta tccgcagcaa aacccaagcc ggttttgacg cgccttatgt accgggttgg
     2401 gactgccacg gcctgcccat cgaagtgatg gtggaaaaac tgcacggcaa agatatgcct
     2461 aaagcccgtt tccgcgaatt gtgccgcgaa tatgccgccg aacagattgc ccgtcagaaa
     2521 aaagacttta tccgcttggg cgtgttgggc gactgggaca atccttactt gaccatggat
     2581 ttcaaaaccg aagccgatac cgtgcgtatg ctcggcgaaa tctacaaatc cggctatctc
     2641 taccgtggtg cgaaaccggt tcagttttgc ttggattgcg gatcttcgct ggcggaagcg
     2701 gaagtggaat acaaagacaa agtatcgcct gcgattgacg ttgcctatcc gtttaaagac
     2761 accgtcgcgc ttgccgccgc attcggcttg gcaggtatcg aaggcaaagc gtttgccgtc
     2821 atttggacga ccacgccttg gactctgcct gcgagccagg ccgtgtctgc cggcgcggac
     2881 gtggtgtatc aattaatcga tacgcccaaa ggcaaattgg tgctggcgaa agatttggcg
     2941 gaaggcgctt tgaaacgcta cggcttttca gacggcatcg ccatccttgc cgaaaccacc
     3001 ggcgacaagc tggaaaacct gcacatgaat catccgttcc tcgaacgcga tattcccatg
     3061 ctcaacggcg aacacgttac caccgatgcc ggtaccggct tggtgcatac tgcgcctgcg
     3121 cacggtttgg aagactacgc cgtctgcaat aaatacggca tcgagcttta caaccctgtc
     3181 aacgccgaag gcaaatacat aagcgaaacg cctcgtgtcg caggcatgag cgtttgggag
     3241 gcgaatcccg tcatcctgca atggccggaa gaaaccggca acctcttggc aagcagcaaa
     3301 atcgaacaca gctacgccca ctgctggcgc cacaaaaccc cgctgattta ccgagcgaca
     3361 ggtcagtggt ttgtcggcat ggacaaagcc ggcagcgacg gtaaaaccct gcgcgacaaa
     3421 gccatcaaag ccgtggacga caccgaattc ttcccgccat ggggtcgtgc gcgtttggaa
     3481 tccatgattg aaggccgtcc tgactgggtg gtttcacgcc aacgctattg gggcacgccg
     3541 atgactttct ttgttcacaa agaaaccggt gaattgcatc cgaactctgc cgaacttttg
     3601 gaaaaagtcg cgcaacgcat cgaagaaaaa ggcatcgagg cttggttctc cctcgataaa
     3661 agcgaattat taagcgcgga agattgcgaa cattacgaca aactccccga taccatggac
     3721 gtatggttcg actcaggctc gacgcattat tccgttgtaa aacaacgcga agaattggaa
     3781 tggccggctg acttgtacct cgaaggcagc gaccaacacc gcggctggtt ccaatcttct
     3841 atgctgaccg gttgcgcctc atccatggga cgcgcaccgt ataaacagct gctgacccac
     3901 ggtttcgttg tggacggcga aggcaaaaaa atgtcgaaat ccatcggcaa cgtcgtcgcg
     3961 ccgcaggaag tgtataacga gttcggcgcg gacatcctgc gtctgtgggc ggcatccacc
     4021 gattacagcg gcgaattggc gatttccaaa gaaatcctca aacgcgtaac cgaaagctac
     4081 cgccgtatcc gcaatacctt gagcttcctg tttgccaatt tgagcgactt taatccgatt
     4141 gaagatgctg ttccacaagc agaaatggtg gaaatcgacc gctacgccct ggtgttggcg
     4201 cgtcaacttc aagaacgcgt tgccggcgat ttttatccgc gttacgcctt ccattttgcc
     4261 gtaaaagaaa tggtttcttt ctgctcggaa gacttgggcg cgttctacct cgacatcctg
     4321 aaagaccgcc tctacaccac caaagccgac agccgcgccc gccgcagcgc gcaaaccgcc
     4381 ctgtaccaca tcacgcgcag cctggttctc ttgattgcac cgattttgtg cttcaccggc
     4441 gaagaagcgt gggacatcat cggcggcggc gaagaagaca gcgtcctctt ccatacttgg
     4501 cacgagttcc cggccatcaa cgaaaaagcc gaagccgaac tggtgaaaaa atggacggca
     4561 atccgcgaag cgcgcgaagc agtaaccgcc gccatcgagc ctttgcgcgc cgacaaaacc
     4621 gtcggttcgt ccttgcaggc ggaagccgaa atcaccgccc cggaagaaat ggccggctat
     4681 ctgaatgctt tgggcgaaga attgcgcttt gccctgctgg tgtctaaagc agaagtgaaa
     4741 gtcggtgatg aacttgccgt tgccgccaaa gccggcgacg gcgaaaaatg cgaacgctgc
     4801 tggcattaca cccgcgatgt gggcgcggtt gcaggttatg aaaccgtctg caaacgctgt
     4861 gcggagaatg tcggcggaga aggcgaaacg cgccattacg cctgataaag tttgagcaaa
     4921 tgccgtctga aaccgccaaa cagtatttca gacggcattt tttgtgccgc gatttgtctt
     4981 cataatggcg gaggggtttt aagattacgg tattgtcggg aatgacggtt cagttgctac
//
LOCUS       DAWTHD010000121         4657 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWTHD010000121 DAWTHD010000000
VERSION     DAWTHD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.121"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            252..1286
                     /gene="pyrC"
                     /locus_tag="WM704_001907"
     CDS             252..1286
                     /gene="pyrC"
                     /locus_tag="WM704_001907"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFA9341256.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="WM704_001908"
     CDS             complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="WM704_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFA9341257.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(1868..2344)
                     /locus_tag="WM704_001909"
     CDS             complement(1868..2344)
                     /locus_tag="WM704_001909"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFA9341258.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(2391..2714)
                     /locus_tag="WM704_001910"
     CDS             complement(2391..2714)
                     /locus_tag="WM704_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFA9341259.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2882..3601
                     /gene="rnc"
                     /locus_tag="WM704_001911"
     CDS             2882..3601
                     /gene="rnc"
                     /locus_tag="WM704_001911"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFA9341260.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            3648..4571
                     /gene="era"
                     /locus_tag="WM704_001912"
     CDS             3648..4571
                     /gene="era"
                     /locus_tag="WM704_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFA9341261.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT          998 a         1169 c         1433 g         1057 t
ORIGIN      
        1 aattgagggc ggaacagtcg gtgcaggcac cgtttgaaac caggcaaatc agctatgcct
       61 ggcgggaatc gggcagccct gaagaaaccg tattgaccgc acaaaccgcc gattccccgg
      121 atttacccgt acaggcagta ttgaacctaa cgcaccgttc ccataacggg aaacccctaa
      181 ttctcccctg acttcagacg gcataaagtc ggcatgccgt ctggaaacca aaaatctaaa
      241 aaggaacaac catgcaaacc ctgaccatca tccgacccga cgatatgcac ctgcacctgc
      301 gcgacggcga cgcgctcaaa gccgttgccc cttataccgc ccgccagatg gggcgcgccg
      361 tcatcatgcc caacctcaaa ccgccagtcg tcagcgtagc cgacgcgctt gcctacaaag
      421 cgcgcattat ggcggcgttg cccgaaggca gcgcgtttga gccgctgatg acgctttacc
      481 tgaccgacca agccacgccc gaactcgtgc gcgaagccaa agccgccggc atcgtcgcct
      541 tcaaactcta ccccgcaggc gcgaccacca attccgattc cggcgtaacc gacctgttca
      601 agctcatccc cgtgttggaa gaaatggcaa aacagggcat cctgttcctc gttcacggcg
      661 aagtaaccga ccccgaaatc gacattttcg accgcgaagc cgcctttatc gggcgcgtga
      721 tgaaacccgt tttggcgcaa gtgccgaatc tcaaagtcgt gttcgaacac atcaccaccg
      781 ccgaagccgc ccgcctcgtt ttggaagcgg gcgacaacgt tgccgcctcc gtgaccccgc
      841 aacacctcct gctcaaccgc aacgacctct tggtcggcgg tgtgcgcccc catcatttct
      901 gcctgcccgt cctcaagcgc gaaacccacc gtcaggcatt ggtcgccgcc gttaccggcg
      961 agaaggcgca caaattcttc ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag
     1021 aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac cgctatcgag ctttacgccg
     1081 aagtgtttga aaaagcaggc gcgttggaca aactcgaagc cttcgcctcg gaaaacggcg
     1141 caaggttcta cggcattccg gaaaacgccg acacgattac ccttgtcaaa caaagccaaa
     1201 ccgttcccgc aagcgtccct tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg
     1261 aaatcggctg gacggtgcag tattgattga acggtcaagc aaaatgccgt ctgaaaggtt
     1321 ttcagacggc atttgtgtat tttccgattc ggattttccg agtatcaacg gcgtttgggt
     1381 tcgtctgggc ggatttgggc ggcgagtttg tcgaggatgc cgttgacgaa tttgtgcccg
     1441 tccgtgccgc cgaaggtttt ggtaacttcg atggcttcgt tgataatgac ggggtagggc
     1501 gtttcgggca tagcggaaag ctcgtggcag gcggtcagca aaacggcgcg ttcgatgggg
     1561 ttgaggtctt tttcgtccct gtcgagcagc gggcggattt tttggatgta gtccgctgcg
     1621 ttggtttgtg tgccgaagaa gagtttgttg aacaattctt cgtccgcttt ggcaaagtcg
     1681 gacatttcgc ggatgttttt agcaatttcg ggcgcggcgg tgcggttgat aagggattgg
     1741 taaacggctt gtacggcaag ctcgcgggaa cggcggcggg ctgttttcat gatttttcct
     1801 tgaaacggtt gggcggcacg gtatgccgtc tgaaacggaa agggcgcatc ggtgtacgcc
     1861 ctgtttgtta ttcttcgtct tcaaactgtt cttcgagcag aaggttgacg aggttggcgc
     1921 attctacggc gactttggcg gcatccgagg ctttttctcc aatccgttca attgcctgcg
     1981 cgtcgttttc ggtggtcagg acggcgttgg caatcgggat gttgtagtcg agtgcgacgc
     2041 ggccgatccc tgcgccggat tcgttggcaa ccagctcgaa atggtaggtt tcgccacgga
     2101 tgacgacgcc gatggcaatc agtgcgtcaa atttttcgga agaggcaaag ttcatcagcg
     2161 cgatggggat ttcaagcgcg ccgggtacgg tggcgacggt gatgttttcg tctgctacgc
     2221 ccaattcttg gagggtgcgg cagcagactt tgagcatttg gctgccgatt tcgttggtaa
     2281 agcgtgcctg tacgatgccg atgcggaggt gtttgccgtc gaggttgggg gcgatggtgt
     2341 tcattgggtg tcctttggta ttcggggttt cggaatgccg tctgaaggtt tcagtcttgc
     2401 ggctgccagt cggcaacggt ttggaatgtg ccgtcttcgg caagctccca cgcgctgcct
     2461 tcgggttggg aaagcagtgc ggcggtttca gggttggttt tggtgatgtc ggcgaggctg
     2521 acgatgctga agttgtcggg gtcgtcggtg tattcgtcgg tttcgtcgcc gctgaagaaa
     2581 cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt aaaggaagcc gacgggccgg
     2641 ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga gtgccgagga aagtgcttgt
     2701 gcaaatgcgt tcattgcggg aatacgttgg gggggaaact tgcggatttt accacgattc
     2761 ccgcgttgtc ggcagacggc ggcggtttgg tggtacaatg tgcgccgttt gcagccttaa
     2821 ggtgtttctg tatttttgga gtatggaaac gcattcgggc tgttttttgc ggaagacggt
     2881 aatgaaagac gatgttttga aacggcaggc acatactgcg atacagaaaa agttaggcta
     2941 cgcgttccgc gatatgtcgc ttttgcggcg ggctttgacc cacaggagcc atcatgcgaa
     3001 gcacaacgaa cggttcgagt ttgtcggcga ttcgattttg aattatacgg tggcgcggat
     3061 gctgtttgac gcgtttccga agttgaccga gggcgagttg tcgcggttga gggcgagtct
     3121 ggtcaatgag ggcgtgctgg cggaaatggc ggcggaaatg aatgtcggcg acggtctgta
     3181 tttgggggcg ggcgagttga agagcggcgg cttcagacgg ccttcgatac tggcggacgc
     3241 gatggaggcg atgtttgccg ccgtcagctt cgatgccgat ttcaacacgg cggaaaaggt
     3301 ggtgcgccat ttgtttgcag aacgcgtccg gcgcgccgat ttccaaaatc aggcaaaaga
     3361 cggcaaaact gctttgcagg aggcgttgca ggcgcgccgt ttcgccttgc cgaaataccg
     3421 catcgaagag caaatcggcc atgccaacga cagtatgttt gtcatttcct gcgatttggg
     3481 cgaactgggt ttcgtgtgcc gtgccaaagg gacgagccgc aaggcggcgg aacaggaggc
     3541 ggcgaaagag gctttgaaat ggctggaaga gaagctgccg ctgaagaaga aaaagaaatg
     3601 aggcggcgcg tgaatatgcc gtctgaaatg tggatatgaa agcgaatatg gatattgaaa
     3661 ccttccttgc aggggaacgc gccgccggcg gataccgttg cggcttcgtg gcgattgtcg
     3721 gtcgtccgaa cgtgggcaaa tcaacgctga tgaaccatct catcggtcag aaaatcagta
     3781 ttaccagcaa aaaggcgcag acgacgcgca accgcgtaac ggggatttat accgacgata
     3841 ccgcgcagtt cgtgtttgtc gatacgccgg gctttcaaac cgaccaccgc aacgcgctca
     3901 acgacaggct gaatcaaaat gttaccgagg cgctcggcgg tgtggatgtg gtggttttcg
     3961 tcgtggaggc gatgcgcctt accgatgccg accgcgtcgt gttgaaacaa ctgcccaagc
     4021 acacgccggt cattttagtg atcaacaaaa tcgacaagga caaggcgaaa gaccgttacg
     4081 cgctggaggc gtttgttgcc caagtgcgcg ccgaatttga atttgcggcg gcggaggcgg
     4141 tcagtgcgaa acacggtttg cggattgcca acctgttgga gctgctcaag ccgtatctgc
     4201 ccgaaagcgt accgatgtat cccgaagaca tggttacgga caaatcggcg cgttttttgg
     4261 cgatggaaat cgtgcgtgaa aaactcttcc gctatttggg cgaggagctg ccttatgcga
     4321 tgaacgtcga agtggagcag tttgaagagg gagacggttt gaaccgcatc tacatcgccg
     4381 ttttggtcga caaagaaagc caaaaggcga ttttgatcgg taaaggcggg gagcgtttga
     4441 aaaaaatttc caccgaagcg cggctggata tggaaaaact gtttgataac aaagtatttt
     4501 tgaaggtctg ggtcaaagtc aaatccggtt gggcagacga cattcgcttc ctgcgcgagc
     4561 tgggtttgta gtttttcttg ctgaacttta cgcaaatgcc gtctgaacag gtttcggacg
     4621 gcattttgtt tcaatcggga atatctttgt taaaaac
//
LOCUS       DAWTHD010000122         4647 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWTHD010000122 DAWTHD010000000
VERSION     DAWTHD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.122"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            563..967
                     /locus_tag="WM704_001913"
     CDS             563..967
                     /locus_tag="WM704_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HFA9341262.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            1007..2188
                     /locus_tag="WM704_001914"
     CDS             1007..2188
                     /locus_tag="WM704_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFA9341263.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(2290..4545)
                     /locus_tag="WM704_001915"
     CDS             complement(2290..4545)
                     /locus_tag="WM704_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFA9341264.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1149 a         1288 c         1216 g          994 t
ORIGIN      
        1 agacggcatt gttctcccgc cgtcattccc gcgcaggcgg gaatccggaa cgtaaaatct
       61 aaagaaaccg ttttatccga taagtttccg tgccgacagg cccggattcc cgcctgcgcg
      121 ggaatgacgg gattttaggt ttctgatttt ggttttctgt ttttgaggaa atgacgggat
      181 tttagtaacc gtaacaactg cctgcgcgac ggctaagggg cttcagcaac cgtagcaact
      241 gcctgtgtgg gaatgacgga caatgggctt cagacggcat ctcttgcctt ccgctaaaac
      301 agtttgccgc acaactgttc aaacgcgtcc gatatgtttc aacacacagg acgacacata
      361 aagcaccgcc ctatatgtcg tcctgattcg gaaggggtta cgcccctccc aaacaaagtc
      421 tgatcctgcc gccctaaagg gcggggtttc aaccgaaaag gaaacacgat gaagtggtac
      481 aattaacggc aatgcggaca gacaaattaa actatcagaa attgccctac cgttttttaa
      541 acactttcag gaataaggaa aaatgaccgc ccaaccctgc cccatctgca cggcacaaga
      601 tgaagatatt ttgctgcaaa cccccaagct gcgcgtcatc gccgtccata acgacagcgg
      661 ttcgccggca ttctgccgcg tcatttggca cggacacatt gccgaaatta ccgacctttc
      721 ggcagcggaa cgcggcgaat tgatggaaat ggtgtacaaa gtcgaagccg ctatgcgcca
      781 agtgttccgg ccggcaaaaa tcaacctcgc cagcttgggc aatgtcgtgc cgcacctgca
      841 ttggcatatt atcgcccgct ttgaaaacga tgcgactttc cctgcgccga tttgggcaaa
      901 ccccgtccga aaacacggta tgaccctgcc gcaaaattgg acggaacagc ttaaaaagct
      961 gctttaaacc cgccgatgcc gtctgaaacc gtatgaaagg gaaattatga ccgaaccgac
     1021 ctcccgccgc cgttttctga aaacctgcac cgccgccggc gcggggctgc ttcaggcttg
     1081 cggcacatcc gccacatccg ttccgcccct tccctcttcc cattccgttg tgaaagcccg
     1141 aaccgtgcct ctccaaacgc cacgccgtca aagttcggac ggcaaccttc tgcgcgttgt
     1201 cgcttcgtca ggatttgccg aagacactaa ccgcgtcaac acagccttaa cccgccttta
     1261 caatgccggt tttaccgtaa ccaaccaaca ggcgggcagc cgccgtttcc aacggtttgc
     1321 cggcacggac gcgcaacgcg cggcagattt ccaagaggtc gcctccggcc gcgtcgccac
     1381 gcctaaagtg ctgatgggct tgcgcggcgg ttacggtgcg gcgcggattc tgccgcatat
     1441 cgattttgcc tcgctcggcg caaggatgcg cgaacacggc acgctgtttt tcggattcag
     1501 cgacgtatgc gccgtccaac tggcattgtt ggcaaaaggc aatatgatga gttttgccgg
     1561 cccgatggct tatagcgatt ttggcaaacc tgcccccggt gcgtttacga tggatgcctt
     1621 tatcaagggg acaacccaaa accgcctgac cgttgatgtt ccttatatcc aacgcgccga
     1681 tgtcgaaacc gaaggcacgt tgtggggcgg caacttaagc gtcctcgcct cgctcgccgg
     1741 tacgccttat atgcccgaca tcgacggcgg cattttgttc ctcgaagatg tcggcgaaca
     1801 gccctaccgc atcgaacgta tgctcaatac gctgtatctt tcgggtattt tgggcaaaca
     1861 gcgcgccatc gtgttcggcg atttccgtat ggaaaaaatt cgagatctct atgattcgtc
     1921 ttatgatttt tccgccgttg ccaagcatat ttcgcgcacg gcgaaaatcc ccgtgctgac
     1981 gggcttcccg ttcggacaca ttgccgacaa aatcactttc cctctgggcg cgcacacccg
     2041 aatccgtatg aacggaaacg gcggctattc ggtcgcgttt gaaggctacc ccacactcga
     2101 tgcgtccgcc ctgactttgg ataccctgct cccaccgccg gatttgccta tcttccccga
     2161 aagcggtgtt gccgatattt cggaataaac ccgcaaacgg acaaatgccg tctgaagcct
     2221 tcagacggca ttttcctgta aaaaaaccgc acctgatggc aggtgcggtt tccattttca
     2281 ggctaaaact tatttgtcgc ggccgaatac gattttagtg gcttggatgg caacgcagat
     2341 tgcaccgccg ataaagatta agtcgggggc agtgcgtacc cagcgcaggg tgtcgaggat
     2401 ttccatttgc aggaattctt cgctgcgggc ataccacaaa ccgtgcgtga tagaggcgta
     2461 tgcctgaatc acgccgacag gcagcaggct gatggcaatc ataccgacca agccgccgtt
     2521 gagcagccag aagccccaag tcatcagttt gtcgtcaaac cgcgcgttcg gtttcaagta
     2581 gcgcgcaacc aacaatacga agcccaatgc caagaaaccg tacacaccga acaaggcggc
     2641 gtgcgcgtga acggcggaag tgttcaaacc ttggatatag aacagggaaa tcggcggatt
     2701 gatcagaaag ccgaatacgc cggcaccgat catattccaa aaagcgactg ccacgaagca
     2761 catcagcggc caacgcaggc gtttcgccca gtcggacagg tgttggtaag accagtgctc
     2821 gtatgcctcg cggcccagca acaccagcgg cacgacttcc aaagcggaga agcaggcgcc
     2881 gattgccata gaggcggagg tagagccgga gaagtacagg tggtgcagcg tgcccggaac
     2941 gccgcccaac ataaagatgg cggcagcggc caaagtggag gcagtggcgg tactgcggcg
     3001 gacaaagccc atattgtaga agacaaaggc aaaggcggca gtggcaaata cttcaaagaa
     3061 gccttccacc cacaggtgga ccacccacca acgccagtat tccattacgg caatcgggga
     3121 tttttcgcca tagaacaggc ccggtgcgta gaacacgccc acgccgacca tagaagcgac
     3181 aaagattgcc agcaggtttt tgtccacgcc tttttctttg aaggcggaaa ccgtgcagcg
     3241 caacatcagg aacagccaca acagcagtcc gaccatcaaa aggagttgcc agaaacgtcc
     3301 caaatcgagg tattcgtaac cttggtgtcc gaaccagaag ttaaattcgg ggggaaggat
     3361 gtgcgtcaac gcgaagaagt tgcccgcgta agaaccgccg accacgatga agagggcgat
     3421 atagaggaag ttcacgcctg cacgttggaa cttgggatct ttgccgccgt tgacaatcgg
     3481 cgcgaggaac aaacctgccg tcaaaaagcc ggttgcaatc cagaagatgg cggattggat
     3541 gtgccaagta cgggtcaggg cgtaggggaa ccagtcggac atttcaaagc ccaacgcctc
     3601 gtcaatgccg tagaaaccct gaccttcgac ggtgtagtgc gcggtcaggc cgcccagcaa
     3661 tacttgtacc acaaacaggg cgaccgtcag gaagacgtat ttgcccaatg ctttttgcga
     3721 aggggtcagt tggattttgg aaatcgggtc ttcagacggc acttccactt cctcgtgttt
     3781 ggtcaggaag gaataacccc acatcaacaa accgatgccc atcaagagca gtacgacact
     3841 ggtaaacgac cacatgtagt tttcagtagt cggtacattg ttgatcaaag gctcgtgcgg
     3901 ccagttgttg gtgtaggtaa agacctcgcc gggacggttg gtcgaagcag accaagaagt
     3961 ccagaagaag aagtcgaaca gtttttcacg cgcttcttgg cttggcaatg tgttgttttt
     4021 cattgcaaag tgttcgcggg tggtttgcaa cttgggatca tcgccgtaaa cgccgtgata
     4081 gtaaggcagg atgctttcga tggctttcac gcgcgtatcg ctgatgacga cgctgccgtc
     4141 ttctttaata cggctttgat tgcggtattc atcggccaag cgggttttca gaacggcttg
     4201 ttcttcaggg gaaacttcat cgaatttttt gccgtaagcc tgttgcgcgg tcaaatccaa
     4261 ccaggcggac aactcacgat gcagccagtc ggccgtccaa tccggagcct gatatgcgcc
     4321 gtgtcccaga atcgaaccga cttccatgcc gccggtagtc tgccacgcag actgacctgc
     4381 caaaatatcg tctttcgtca tcagcacttt gcctgatgcg gaaacgacct gttcggggta
     4441 aggcggggct ttcttataaa cctcgctgcc catatagcca agaatggtaa agcataccgc
     4501 cagaacggca aacagcaagt accacagctt cttgtactgt cccattttga gagctccttt
     4561 taataaatca gttgtttaaa attcacaaaa tatgaatgtt aaagattgta gcatggttta
     4621 ccgcgcaaat aaacatttgt tcaaaca
//
LOCUS       DAWTHD010000123         4496 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWTHD010000123 DAWTHD010000000
VERSION     DAWTHD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.123"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            158..1672
                     /gene="katA"
                     /locus_tag="WM704_001916"
     CDS             158..1672
                     /gene="katA"
                     /locus_tag="WM704_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFA9341265.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2188..2753
                     /locus_tag="WM704_001917"
                     /pseudo
     CDS             2188..2753
                     /locus_tag="WM704_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            2975..4138
                     /locus_tag="WM704_001918"
     CDS             2975..4138
                     /locus_tag="WM704_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFA9341266.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
BASE COUNT         1100 a         1190 c         1177 g         1029 t
ORIGIN      
        1 gtataatcgc atccatagtt tttgcaaaag gaaaataaaa tgtcgattca agaaatttat
       61 tacaatcaag aaaccggtta cgaatacgct ttccgccaaa tcgtactgta agccgtttgc
      121 cccccatttg accaacctga gaaaaggaac aagagcgatg actacctcca aatgccccgt
      181 aacccatctg accatgaaca acggcgcgcc cgttgccgac aatcaaaaca gcctgaccgc
      241 cggcccgcgc ggccccctgc tgacgcaaga tttgtggctg aatgaaaaac tcgccgactt
      301 cgtgcgcgaa gtcatccccg aacgccgtat gcacgccaaa ggttcgggcg cgttcggtac
      361 gtttaccgta acacacgaca tcaccaaata cacccgcgcc aaaattttca gcgaagtcgg
      421 caaaaaaacc gagatgttcg cccgtttcac caccgtggca ggcgagcgcg gcgcagccga
      481 tgcggaacgc gacatccgcg gttttgcgtt gaaattctat accgaagaag gcaactggga
      541 tgtggtcggc aacaacacgc ccgtgttctt cctgcgcgac ccgcgcaaat tccccgacct
      601 gaacaaagcc gtcaaacgcg atccgcgcac caatatgcgt tccgccacaa acaactggga
      661 cttctggacg ctgctgcccg aagccctgca ccaagttacc atcgtgatga gcgaccgcgg
      721 tattcccgcc agctaccgcc atatgcacgg cttcggttcg catacctaca gcttctggaa
      781 cgaagcgggc gaacgttttt gggtgaaatt ccatttccgc agccagcaag ggattaaaaa
      841 cctgaccaac gaagaagctg caaaaatcat cgccgacgac cgcgaaagcc atcagcgcga
      901 cctgtacgaa gccatcgagc gcggcgagtt cccgaaatgg acgatgtaca tccaagtcat
      961 gcctgaagcc gatgccgaaa aagtgcctta tcatccgttt gacttgacca aagtttggcc
     1021 gaaaaaagac tatccgctga ttgaagtggg cgaattcgag ttgaaccgca atcccgaaaa
     1081 cttcttcgcc gatgtggaac aatccgcctt cgcaccgagc aacctcgttc ccggtatcgg
     1141 cgcgagcccg gataaaatgt tgcaagcccg tttgttcaat tacgccgacg cgcaacgtta
     1201 ccgcttgggt gtgaacttcc gccaaatccc cgtcaatcgt ccgcgttgcc ctgttcacag
     1261 caaccagcgc gacggacaag gccgcgccga cggcaactac ggcagcctgc cgcactacga
     1321 acccaacagc ttcggccaat ggcagcaaca acccgacttc gccgaaccgc ctttgaaaat
     1381 caacggcgac gcggcacact gggactaccg ccaagacgat gacgactact tcagccaacc
     1441 gcgcgccttg ttcaacctga tgaacgacgc gcagaaacag gctttgtttg acaacaccgc
     1501 cgcagccatg ggcgatgcgc ccgacttcat caaataccgc catatccgca actgctaccg
     1561 ttgcgacccg gcatacggtg aaggcgtagc caaagccctc ggactgaccg tcgaagacgc
     1621 acaagccgcc cgcgcgaccg atcccgcact gggtcaggcc ggtttgctgt aagggggcat
     1681 tatgtggatg gaaattgaag aaatcctgtc ccgcgccgtc cgataccgga aataaccggg
     1741 cataaaaatg ccgtctgaaa cgtcgtttga ccgtttcaga cggcatttcc acacctcgcc
     1801 ccgccgtttc agtgggcgtt ttttattata gtggattaaa tttaaaccgg tacggcgttg
     1861 cctcgccttg tcctgatttt tgttaatcca ctataaacgc aaaatatccc gtcattcccg
     1921 caccatcccc accggttcaa accggcacga aaacttttcc gcgtcattcc cgcgaaagtg
     1981 ggaatccgga acgtaaaatc taaagaaacc gttttatccg ataagtttcc gtgccgacgg
     2041 ggctggattc ccactttcgt gggaatgacg ggatttctgt ttttgatttt ttgtttttgc
     2101 gggaataacg ggatgcgggt tttcgtgcgg cgtttttcgc gctttgctgt tttgttataa
     2161 tccgcccttt ttaaggatgg gtgcggtatg agtctttacg ctttgctctt ggttgcgctg
     2221 ggaatgtcga tggatgcgtt tgccgtcgcg ctggcaaagg gtgcggcggt caggatgccc
     2281 cgcgcaaaat tgcagcaacg gctttggtgt tcggtacggt tgaagcgttc atgccgctgg
     2341 cgggctgggt aggcggtttt tatgccaagc cgtttatcag cgaatgagac cattgggtgg
     2401 cttttgtcct gttgggcgga ctgggtctga aaatgatgcg cgaagggttg tccggcgagg
     2461 cggaagatgt gcgcgaaagc aaacaggaaa gcctatggat gacggttttg actgcttttg
     2521 gaaccagtat tgattccatg atcgtcgggg tgggcttggc gtttatggag gtaaacatcg
     2581 cctttgccgc cgccgtaatc ggtatggcgg ctacggtaat ggttaccatc gggctgacgg
     2641 cgggaaaggc ttttggcgta ttgttcggca ggcgtgcgga atttgccgga ggtttggtgt
     2701 tgattgccat cggtacatgg acgctcttat cgcatttggg tttgattcaa tgatgtcgga
     2761 aaataaaatg ccgtctgaag ctttaccggt ttcagacggc attttgttaa acttgattct
     2821 ctatcggtat tattgtgtaa agctattgaa aataaaatat tgattttccg caaagatggc
     2881 taaaggtgcg ataggtacac tgaagaaata atcggcagaa caggtttgcc tgccggtttg
     2941 aacaatgatt ttaacctccc ataaggaata cacgatgtct ttcaaactcc gttacctcgc
     3001 atcagtattg gcattgtctt cactgttggc ggcatgcggc ggtcaggaaa agtctgcggc
     3061 aggcgatgct tctcctgctt ccgagaccga agcggcttcc caagtacagg cctcggaagc
     3121 cgttccttcc gcttcatcgg cctcgcccga agaccaagac cttttgaaac gcgcgcaagg
     3181 tgtattccaa cctttgccga ctgtcgaaga aatgcagaaa atccgtccgt ttaccgaaga
     3241 acaggtcaaa ctcggacacc aactctggta tgaaccacgc ctttccaaag gcaataccgt
     3301 aagctgcaac tcttgccaca accttgcttc cgccggtgtg gacaatatgc cgaccagtca
     3361 ggggcacaaa gggcagttcg gcggacgcaa ctcgcctacc gcattgaatg ctgcgctgct
     3421 gggcagccag ttttgggacg gacgtgccgc cgatgttgaa gaacaggctg gcgggccttt
     3481 ggtgaatccg gtggaaatgg cgaatgattc gcaagaggcg gctgcagcca aaatcgccaa
     3541 agttcccgaa tatcaagaaa tgtttaaaaa agcttttcct gaagacggcg cggtttcgtt
     3601 taaaaacatc actaccgcat tgggtgcgtt tgagcgtacc ctgctgacgc cgaccaaatg
     3661 ggacgaatac ctcaaaggca acgtcaacgc cctgagcgaa caggaacgaa aaggcgtgcg
     3721 cgcgttcatg gacaacggct gtattgcctg ccacaacggt gtcaaccttg gaggcacgac
     3781 cttccagaaa ttcggtctgg tccaagggcc gtattggaaa ttcattgaag atccgaaacg
     3841 cgacaaaggc cgtgctgacg taaccaaaaa aaccgaagac gaatttttct tccgtgttcc
     3901 ggggttgcgt aacgtggcta aaacttatcc gtatttccac aacggcagcg tgtgggagct
     3961 ggataaggca gttaccatca tgggtaaggc gcaattgggt aaagacattc cgaaagaaga
     4021 tgtggataac atcgtcgtat tcctgaatgc actttccggc aatgtttccg aatcagcgcg
     4081 cacgatgccg gaactgcccc tgaccgcacc gatggaatct aagccggaca acaaataatc
     4141 cggccggtac gcaaaaatgc cgtctgaaac gggaatctgt ttcagacggc attttatatt
     4201 tcgtcgcgtc agatacgcag ggtatgccgt agggtgtgct tcagccagcc gtttccacca
     4261 attcaaccaa ccatgtccgg gtcttatgcg tttttcacga atgcgcggcg taggcggagg
     4321 gattggtggg ctgaagccta attcaatggc tgcgttgtgt aagccatgat gcgtttggat
     4381 aattaatcaa tattcttgcg ctaccattac catcggcttt gggggtttgt acttcggcat
     4441 tatgataatc aatagcctgt ttggattgta ttcccgaatc cgacagtttt agatta
//
LOCUS       DAWTHD010000124         4466 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWTHD010000124 DAWTHD010000000
VERSION     DAWTHD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.124"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(72..791)
                     /locus_tag="WM704_001919"
     CDS             complement(72..791)
                     /locus_tag="WM704_001919"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFA9341267.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1008..1862)
                     /locus_tag="WM704_001920"
     CDS             complement(1008..1862)
                     /locus_tag="WM704_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HFA9341268.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1991..2719)
                     /gene="rpsB"
                     /locus_tag="WM704_001921"
     CDS             complement(1991..2719)
                     /gene="rpsB"
                     /locus_tag="WM704_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFA9341269.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2892..3125)
                     /locus_tag="WM704_001922"
     CDS             complement(2892..3125)
                     /locus_tag="WM704_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341270.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            3203..3784
                     /locus_tag="WM704_001923"
     CDS             3203..3784
                     /locus_tag="WM704_001923"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFA9341271.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3845..4435
                     /locus_tag="WM704_001924"
     CDS             3845..4435
                     /locus_tag="WM704_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9341272.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          955 a         1047 c         1289 g         1175 t
ORIGIN      
        1 tctccatata ataaatggct gtctgaagcc catatgcgac tatatctgcc gaccctatgg
       61 tcaatcatca atcagcagtg gaccagtgtg ccttcgtctt cgccggtaat gacgcgtttg
      121 agcgagcctt gtttggcgat gccgaagacg acgatattga gcttgcgttc gcggcagagg
      181 gcaaaggcgg tcgcgtccat gactttgagg tttttattca aggcttcgtc aaaagtaatg
      241 gtttcgtagc gtgtggcgga cgggtctttt ttcgggtctg cggtgtacac gccgtcgacg
      301 ttggtggctt tgagcatcac gtcgcagttc atttccgcgc cgcgcaatgc ggcggcagtg
      361 tcggtcgtga agaacggatt accggtaccg gcggcgaaaa tcacgacttt gccttcttcc
      421 aaatattgaa tggctttagg acgggcgtaa gtttcagcga tttgctgcat agacagtgcg
      481 gattgtacgc gcgctttgat gcccaaggtt tcaaatgcgt ctttgagtgc caaggcgttc
      541 atcacggtcg ccatcatgcc catgtagtcg gcagtggcgc ggtccatgct gcctgcttgt
      601 gcagatacgc cgcggaaaat gttaccgccg ccgacaacga taccgacttg cacacccatt
      661 ttcacgattt cggcaatttc gccgacagtt tgaacaatgg tatcgtgatt gatgccgaac
      721 ggatcggaac ccatcaggga ttcgccggag agtttcagta atacgcgttt gtatttgatt
      781 tgctgtgtca tgggatacct tgctttcttg aatgtcgtcg gtcggagttg ggacgtctga
      841 atttcaaatg gtcggattac aatgatatgg tgtgtcgttc tattattgtt ttgttttcag
      901 acggccttta tattcggtcg tcttaaaata ggtaccgtaa acggttttct caaaaaaaag
      961 caccctgatt cgtttggaat ccaggtgctt tctttttcat aagtgcctta cactttggcg
     1021 gctgcggcta cttcggctgc gtagtcgaca acggctttct cgataccgtc gcctactttg
     1081 tagcgtacga agctaaccac ttcagtgtcg ttttctttag cgaattgggc aacagtttgg
     1141 tcagggttca tcacgaatgc ttggccgttc agagtgattt cagccaagaa tttgcggata
     1201 cggccttcaa ccattttagc ggcgatgtcg gcaggtttgc cggaagcgat ggcttgctcg
     1261 gtgtagatgt ggcgttcttt ttcaacggtt tcagcatcta cttcggcttc gcttacgcat
     1321 tgcggtttgg cggcaacgat gtgcataccg attttgcgtg ctacatcttc agagcctttg
     1381 tactcgacca aaacgccttc ggtcgccaat gcgccgtgga tgtaggcaac cagttggttg
     1441 gcagtgtcaa tcacttggaa gcggcggaca gacatgttct cgcccaattt agcgatgatg
     1501 gctttgcgtt ctgattcaac cagttcgctc agttcttcaa cagaagccgg ttttttctcg
     1561 gcagcagttt tcgcaacgaa gttggcaaat tctacgaagc cggcgtcttt agcaacgaag
     1621 tcggtttcgc agtttacttc gaccaatgcg ccgacattac cgttgattgc ataagccaat
     1681 acgccttcgg cagcagtacg gccagccagt ttgcccgctt tcgcaccgga tttaatacgc
     1741 aggatttctt cggctttgtc gaagttgcct tcagcttcaa ccaaggcttt tttgcattcc
     1801 atcataccca ggccggtagc ggcgcgcagg tcggcaacca tttttgcagt aatttctgcc
     1861 attttgaatc tcctagattt ttgggaaata cggaaaggat tttccacatt cgggaatcgt
     1921 gtgccgcgaa cgggcgttca gacggcatat ttgagaaaag gggcataacg cccctcttct
     1981 gtgtaccgaa ttactcggca gcggtttctt gggcagcggc tacggtttct tgcagcgctt
     2041 ggtttttgcc ttccaaaact gcgtcggcga tgcctcggca gtacaggcgg atggctttgg
     2101 cggagtcgtc gttgccgggg atgacgtatt tcacgccgtc tgggctgttg ttggtatcga
     2161 ctacggcgat aacggggatg cccagttttt cagcttcaac cagagtacct ttttggtagc
     2221 cggtatcgat aacgaaaatc gcgtcgggca ggcctttcat gtttttgata ccgcccaaag
     2281 agcgttccag tttttcaacg tcgcgttgca tttccagaat ttcttttttg ctgaaaccgc
     2341 tttcggcagc attttccaag gctgcggttt tttcttccag gcgtttgatg gattgcttaa
     2401 cggttttgta gttggtcagc ataccgccca accagcggtg gtcgacgaaa ggcataccgg
     2461 cgcgggtcgc ttcttcgcgg atgatgtcgc gtgcctggcg tttggtacct acgaacaata
     2521 ccgtaccttt gttggcaacc agacgacgta cggcttcttg cgcctcttgg aacatcggca
     2581 gggttttttc caagtttacg atatggattt tgttgcgcgc gccgaaaatg tattgagcca
     2641 ttttcgggtt ccagaaacgg gtttggtgac cgaagtgaac accggcttca atcatctgac
     2701 gcatagtaat ttgagacatg ttatttcctt gaaagggtta aagagcacac attcaatcgc
     2761 atagaacttg ataggcggcg cctattgtca agcacccttc ggcgaaagtg ggcataagtt
     2821 ttaaaaatag tgtgtttccg aaatggaaaa ctgacggatt atagcggata agatgccgtc
     2881 tgaaaaggga ttcagggttt tgtgccgctg tctttttgcc gttgtgcgcg ctgctgttcg
     2941 gattctgcct gttcctgctg ccccatcagg cggttgcggc ggcggacggt tacggcgaag
     3001 accgtgaaca cggtgggcag aaccacccaa aaaaccaaat aaatcaaagc gcgcgcaata
     3061 ctcggctgcg cggcggaata cataacggca acaaaaagat agccgatggc gacgatgtgc
     3121 cacattgtgt gcgtcctgtt tgaatgttaa caaaatcgtt ataatcgcaa cattctaccg
     3181 caatcctgaa aggccgtctg aaatgaggaa cgaggaaaaa cgcgccctgc gccgcgaatt
     3241 gcgcgggcgg cgttcgcaaa tggggcggga cgtgcgggcg gcggcggcgg taaaaatcaa
     3301 ccgcctgctc aaacgttata tcaagcgcgg tcggaaaatc ggcgtgtatt ggccgatggg
     3361 caaggaattg cgtttgggcg gctttgtccg cgcggcgcaa aaacgcggcg caaaactcta
     3421 tctgccttat atcgaaccgc acacgcggcg gatgtggttt acgccgtatc ctgaacgcgg
     3481 aatggaacgg gaacgcaagc gcggtagggc gaagctgcat gtccctcagt ttgcagggcg
     3541 caaaatccgc gtgcacggtt tgtcggtatt gctcgtcccg cttgtcggca tagaccgcga
     3601 aggctaccgt ttggggcagg caggcggcta ttacgatgcg acgctttcgg cgatgaaata
     3661 ccgtttgcag gcgaaaaccg tgggcgtggg ctttgcctgc cagttggtgg acaggctccc
     3721 acgcgaggcg cacgacctgc cgctggacgg ttttgtatcg gaagcgggga tattgtgttt
     3781 ttagccgttc agacggcatt tcgggcggaa atgccgtctg aaggcggaat attgggagaa
     3841 aaggatgtcg gaaaggataa tactgccggt tttgtcattg ggcggcgtac ggctcgaacc
     3901 gttggatgtg catcatgaaa cgggtttgcg cgaggcggtt tgcgatgggg aggtttggaa
     3961 gctgggggta acgtccgcgc cccatccgga tagggtggca gactatatcg ggacagcatt
     4021 ggcaacgcgt ttggcgtttg cggttgtcga tgaagaggcg gacagggtgg tcgggacaac
     4081 ggcgtattat cattttgaac cgcagattcc gcgtttggat atcggattca catggtatgc
     4141 ggcatcggca cggcggaaac ggataaacac ctgttgcaaa atcatgctgt tggattacgc
     4201 atttgatgtt ttagcttgct gttgcgcggg atggcgcacg gatattctga accttgcctc
     4261 gcaacgtgcc atcgaacggc tgggcgcgga aaaagacggc gtgctgcgta tgcatatgct
     4321 ccgtaaagac ggcagtgtgc gcgatacggt tgtgtacagt atgctgaggg aagattggtg
     4381 caaaaacagg gaaatcctga ccggcaggtt ggcagggtat ggcgtgcagg tgtgaggcag
     4441 tattctcaaa tttaaaaaac ttaacg
//
LOCUS       DAWTHD010000125         4413 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWTHD010000125 DAWTHD010000000
VERSION     DAWTHD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.125"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..272
                     /locus_tag="WM704_001925"
     CDS             <1..272
                     /locus_tag="WM704_001925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341273.1"
                     /translation="TDPKTNHILKQIISKSDFFKIKFQKKETGKIRHKTLTYSKPSVL
                     QRKYPKKTICLPSVSNRYTAGTRYFSDGAAVVRTDKNVTAAERIA"
     gene            326..2710
                     /gene="ppsA"
                     /locus_tag="WM704_001926"
     CDS             326..2710
                     /gene="ppsA"
                     /locus_tag="WM704_001926"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFA9341274.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2931..4190
                     /gene="rho"
                     /locus_tag="WM704_001927"
     CDS             2931..4190
                     /gene="rho"
                     /locus_tag="WM704_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFA9341275.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIILLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
BASE COUNT         1156 a         1314 c         1052 g          891 t
ORIGIN      
        1 gtacagaccc aaaaacaaat catattttaa aacaaattat ctcaaaatca gatttcttta
       61 aaataaaatt ccaaaaaaaa gaaacaggaa aaatacggca taaaacatta acctattcaa
      121 aaccatctgt tttacaaagg aaatacccga aaaaaacaat atgcctgcca tcggtttcaa
      181 accgctacac agccggaacc cgctattttt cagacggcgc agcggttgtc cggacagata
      241 aaaacgtgac agcggcggaa cgaatcgctt aaaataagca agcggatttg tttctttttt
      301 aacatatttt ggattggaca cacaaatggc cgacaactac gtaatctggt ttgaaaacct
      361 gcgtatgaca gatgttgaac gcgtgggcgg taaaaacgcc tcgctgggcg aaatgatcag
      421 tcaactgacc gaaaaaggcg ttcgcgtccc cggtggcttt gccaccacag ccgatgccta
      481 ccgcgcattt ctcgcacaca acggtctgaa cgaacgcatt tccgccgctc tggcaaaatt
      541 ggatgtcgaa gacgtttccg aactggcacg cgtcggcaaa gaaatccgcc aatggatttt
      601 ggacacgccc ttccccgaac agctcgatgc cgaaatcgaa gcggcatgga acaaaatggt
      661 taccgatgcc gacggcgcgg acatttccgt tgccgtacgc tcttccgcaa ccgccgaaga
      721 cctgcccgac gcatcatttg ccggccaaca ggaaaccttc ctgaatatca atggcttgga
      781 taacgttaaa aaagcgatgc gccacgtttt cgcctccctg tacaacgacc gcgccatttc
      841 ctaccgcgtc cacaaaggct tcgaacacga catcgtcgcc ctttccgccg gcgttcaacg
      901 catggtgcgt tccgacagcg gcgcatcagg tgtgatgttc accctcgaca ccgaatccgg
      961 ctacaaccaa gtcgtctttg ttacctcctc ttacggtctg ggcgaaaacg tcgtacaagg
     1021 cgcggtcaac ccggacgaat tttatgtgtt caaacccacg ctcaaagcag gcaaacccgc
     1081 catcctgcgc aagaccatgg gttcgaaaca catcaaaatg attttcaccg acaaagccga
     1141 agccggcaaa tcggtaacca acgtcgaagt ccccgaagaa gaccgcaacc gcttctccat
     1201 taccgacgaa gaaatcaccg agttggcaca ctacgcgctg accatcgaaa aacactacgg
     1261 ccgcccgatg gatatcgaat ggggacgcga cggcctggac ggcaaactct acatcctgca
     1321 agcccgcccc gaaaccgtca aatcccaaga agaaggcagc cgcaacctgc gccgctacgc
     1381 catcaacggc gaaaaaaccg tcttgtgcga aggtcgtgcc atcggtcaaa aagtcggtca
     1441 gggcaaggtg cgcctgatta aagatgcttc cgagatggat tccgtcgaag ccggcgacgt
     1501 actggttacc gacatgaccg atccggattg ggaacccgtg atgaaacgtg cttccgccat
     1561 cgtgaccaac cgcggcggac gcacctgcca cgccgccatc atcgcgcgcg aactgggtat
     1621 tcctgccgtc gtcggctgcg gcaatgcgac cgaactcttg gaaaacggtc aggaagttac
     1681 cgtatcctgt gccgaaggcg ataccggctt tatctatgcc ggcctgctgg acgtacaaat
     1741 taccgatgtc gccttggaca atatgccaaa agcacccgtc aaagtcatga tgaacgtcgg
     1801 caatcccgaa ctcgcattca gcttcgccaa tctgcccagc gaaggcatcg gcttggcgcg
     1861 tatggaattt atcatcaacc gccaaatcgg catccacccg aaagccttgt tggaatttga
     1921 caaacaagac gacgaactga aagcggaaat tatccgccgc atcgccggtt acgcatcccc
     1981 tgtcgacttc tacgtcgata aaatcgccga aggcgtggca acgcttgccg catcggttta
     2041 tccgcgtaaa accatcgtcc gtatgtccga cttcaaatcc aacgaatacg ccaacctggt
     2101 cggcggcagc gtatacgaac cgcatgaaga aaacccgatg ttgggcttcc gtggtgcggc
     2161 gcgttatgtt gccgagagct tcaaagactg tttcgccttg gaatgcaaag ccttgaaacg
     2221 cgttcgtgac gaaatgggtc tgaccaacgt cgaaatcatg attccgttcg tccgcacttt
     2281 gggcgaagcc gaagcagtgg ttaaggcatt gaaagaaaac ggcttggaac gcggcaaaaa
     2341 cggcctgcgc ctgattatga tgtgcgagct gccgagcaac gcggtattgg cggaacaatt
     2401 cctgcaatac ttcgacggct tctccatcgg ctcgaacgac atgacccagc tgaccctcgg
     2461 cctcgaccga gacagcggct tggtgtccga atcgtttgac gaacgcaacc ctgccgtcaa
     2521 agtgatgctg catcttgcca tctccgcctg ccgcaagcag aacaaatatg tcggtatctg
     2581 cggtcaaggt ccgtccgacc atccggactt tgccaaatgg ctggttggag aaggcatcga
     2641 aagcgtgtct ctgaacccgg ataccgtcat cgaaacttgg ctgtatttgg cgaatgaatt
     2701 gaacaaataa tcaattccca tacccccaag cctgaaaagc gcgggggtat tttttccaag
     2761 cagctccgtt cagacggcat ttcctgtcga tgccccgtcc gcgataatat ttgacaccca
     2821 cgcgccgact gcctacaatt cccccctctc cgagcaaccg gcaacagtca gcttcttctt
     2881 tcagacggca tccacccgtc ttttcctttc tattctatat atcattgatt atgcacgtct
     2941 ccgaattaca aaccctgcac atttccaaac tcttagaatt ggcggaagaa cacggcatcg
     3001 aaaacgccaa ccgattccgc aaacaagacc tcgtatttgc catcgtccgc cagatgatga
     3061 aaaaaggcga gggttttacc tgctccggca cgctcgaaat cctgcccgac ggcttcggct
     3121 tcctccgcag cgcggacacg tcctatcttg ccggccccga cgacatctat gtctcgccca
     3181 ctcaaatccg ccgcttcaac ctgcatacgg gcgacaccat cgaaggcagc gtgcgcgtcc
     3241 ccaaagacaa cgaacgctat tttgccttgg tcagactgga ttccatcaat ggcgaccacc
     3301 cggaagtatg ccgccataaa atcctgtttg aaaacctgac cccgctgttt ccgaccgaac
     3361 agttgaagct ggaacgcgac ttaaagtccg aagaaaacct gaccggacgc gccatcgacc
     3421 tgatttcgcc catcggcaaa ggtcagcgcg ccctcttggt tgccccgccc aaaatcggta
     3481 aaaccgtgat gctgcaaaac attgcccacg ccgttaccgc aaactatcct gaagtcgaac
     3541 tcatcatcct cttgattgac gaacgccccg aagaagtaac cgaaatgagc cgttccgtcc
     3601 gaggtgaagt agtctcctcc acctttgacg aaccggcgca acgccacgtc caagttgccg
     3661 aaatggtgct tgaaaaagcc aagcgtatgg tggaacacaa aaaagacgtg gtcatcctgc
     3721 tggattcgat tacccgcctt gcccgcgcct acaataccgt cgtccccgcc tcgggcaaaa
     3781 tcctgaccgg cggtgtcgat gccaacgcgc tgcaccgccc caaacgtttc ttcggtgcgg
     3841 cgcgcaacgt ggaagaaggc ggttcgctga ccattatcgc caccgcattg gttgaaaccg
     3901 gcagccgtat ggacgatgtg atttacgaag aattcaaagg taccggcaat atggaattgc
     3961 acctcgaccg ccgtatggcg gaaaaacgcc tcttccccgc catcaacatc aacaaatccg
     4021 gcacgcgccg cgaagagctg cttgtgccga acgaccagtt gcaacgtatg tggttactgc
     4081 gcaagttcct gcacccgatg gacgaaatcg aagctaccga gttcttaaac ggaaaaatca
     4141 aagcatctaa aaataatgat gatttctttg aactgatgcg cggaaaataa acgcgccgcc
     4201 cgcattaatg cgaaatgccg tctgaagcct gaaaatcggg tttcagacga cattttcatt
     4261 cgcacggccg gcgcagctcc ccccccgcta acggcgcaac cgccggcagt gtccgtgtcc
     4321 gcctgccgaa ggcgcggcct ttgcaaaacc ggctcgaacg catccctacc ggcaatgaaa
     4381 accgatgcgg gaacttgatt caaggttgcg ccg
//
LOCUS       DAWTHD010000126         4387 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWTHD010000126 DAWTHD010000000
VERSION     DAWTHD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.126"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..1214)
                     /locus_tag="WM704_001928"
     CDS             complement(<1..1214)
                     /locus_tag="WM704_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341276.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGGVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKKELNV"
     gene            1411..1486
                     /locus_tag="WM704_001929"
     tRNA            1411..1486
                     /locus_tag="WM704_001929"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Glu,seq:ttc)
     gene            complement(1702..1854)
                     /locus_tag="WM704_001930"
     CDS             complement(1702..1854)
                     /locus_tag="WM704_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341277.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            1914..2720
                     /gene="thiD"
                     /locus_tag="WM704_001931"
     CDS             1914..2720
                     /gene="thiD"
                     /locus_tag="WM704_001931"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFA9341278.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2778..3647)
                     /gene="tehB"
                     /locus_tag="WM704_001932"
     CDS             complement(2778..3647)
                     /gene="tehB"
                     /locus_tag="WM704_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFA9341279.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3760..4197
                     /gene="rnhA"
                     /locus_tag="WM704_001933"
     CDS             3760..4197
                     /gene="rnhA"
                     /locus_tag="WM704_001933"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFA9341280.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1009 a         1236 c         1039 g         1103 t
ORIGIN      
        1 acattcagct ctttcttttt cttttcaatc gactttgtat cgggggttaa ttcatatccg
       61 tccgcatcca cggttaaggc aaacatatct ttccctttgg caaagctcct tttcccgata
      121 tgggcttccc cttcaaaata agagtctttg ttggcaaggt tcacgctcac gctgccgccg
      181 ttgtaggcga aaatatcgcc tttgattttg acaaccttgc cttccccgcc gacaacctcg
      241 actttgctgt atttcccgtc aatcgcaaac gcgctgccta tgcttttggg cctgccttgc
      301 tcgtcataaa ccggcgttgc aacgatatcg acgttcccac ggaaaatcgc ttccatctta
      361 gaaccttccg gaatctgctt cttattgcct aatttgcggc ttgaatagaa gccaaaaccg
      421 ttgctttccg cctcttcctg tccgttccta tcgattttaa tgtttacacc gcccttgaac
      481 tccattgtgg tgatgtttgg agaagccttg ttgaagttga tgtctgagtt accatagtgg
      541 ttggcggtaa tgccctgtcc tttatctttt cctgatttga tgttgatatt cagcgtcgag
      601 ccttcttcgg aaacaagacg atattctccc attttttcac cattattaag caataggata
      661 ccttcgttac tagagttggc atttgagtcc gcgtccatat tgatggtatt gcgcccttta
      721 aaggcaagca tcgccggcgc caagctactg gatttatgcc tatcatcaga caaaatgccg
      781 gcaggcgcgt tatgggcggc tatggttacg ttttcaaaag taaccttatt ttctccctca
      841 tagctttgat ccaccaacat caccgcgccg ctccaattct gccatgcgta cgtgtaaccg
      901 tttttattta tatttctttc aatcgagtta ttgtcaatcg tcggcggggc atcggcagga
      961 ccgtctgcca atttgccttt tatcgtaatg ttgttcgtac cgccgttggc ggcaatgatg
     1021 agggaattta agggtttgac ttcggttccg ccattaaggg tatagttgtt ctcttgccct
     1081 tgttttgcga ggacgagggt gtatttgtct gtcttctcta tcgttccact gtttgcagcg
     1141 gccgcccctt gcgcgtgcat agaagcggcc gcgaagccaa gtaagatgca ctgaactgtc
     1201 ttcttttgtt tcatcggttt ttccattttg aaggtatgtg atttttatca ttatatgctt
     1261 aaatcattga cacgccccca aaaaaaatga attgcttgat attccaatgt tttttatctt
     1321 gtgatacggt cagacaaacg ccccctgaca ttcgtttaga cggcatcgta ttgctaaatt
     1381 tctataagta tgtataatgt ccgtttccac gcgcccatcg tctagaggcc taggacactg
     1441 ccctttcacg gcggcaaccg gggttcgaat ccccgtgggc gtgccaattc aaaaacctgc
     1501 ttgtttcaag caggtttttt attatgaatc gttattcccg caatttttcg tcattgccgt
     1561 gaaaacggga atctagaacc tcaaactttc agataatctt tgaatattgc tgttgttcta
     1621 aggtccggat tcccgcctgc gcgggaatga cggaatttta ggtttctgtt tttgcgggaa
     1681 tgacgaatga agcgtgccgg tttatactcg ccgcaacacg cggttcagac ggcatcgccc
     1741 tcttttttca ttatcagttg ctacgatgca ccccgctgcc ttgccctgtg ccttgtcctg
     1801 caatacgaca taataatgca ccacaaaccc cgcgctgcgg ttttcagacg gcattgcctg
     1861 cttttttaca ggcagtagcc ctttttatcg gacgcaatat taaggaggaa caaatgaaag
     1921 gctcttttgt gcagacgctt gccatcgccg gttcggattc gggcggcggt gcgggcattc
     1981 aggcggattt gaaaacgttt cagatgcgcg gcgtgttcgg aacgtgtgtc atcaccgccg
     2041 ttaccgcgca aaataccttg ggcgtgtcgg cggttcatct cgtcccgacc gaaaccatca
     2101 ccgcacaaat ccaagcaatc agggaagact ttgacatccg cgcctacaaa atcggtatgc
     2161 tcggcacggc ggaaatcatc gaatgcgttg ccgacaagct gaaacactgc agctttggca
     2221 ggcgcgtact cgaccctgtg atgattgcca aaggcggtgc gccgctgttg caggattccg
     2281 ccgttgcggc actgacgcgc ctgctgctgc cggacacgga catcctgacc cccaacctgc
     2341 ccgaggcgga agccttgacg ggcgtgcata tcgaaaaccg taaagatgcg gaacgtgcgg
     2401 caaaaatcct gcttgattac ggtgtcaaaa atgtcattat caaaggcgga catttgaacg
     2461 gcagcacaag cggacgctgc acggattggc tgtttacgca aaacgaaacg ctggaattgg
     2521 acagcccgcg ctttccgacc gcccacacgc acggcacggg ctgcacgttt tccgcctgca
     2581 ttaccgccga gttggcaaaa ggcttagacg tttgcaaagc cgtacagact gccaaggcct
     2641 acatcacggc ggcaatctca aaccctttgg aaatcggcgc gggacacggg ccggtcaatc
     2701 attgggcgta tcgggactag acgtaaaaat gccgtctgaa cgccttttcg ggttcagacg
     2761 gcatcgtccg gcgcggctta tcctggtttt ttcgccagca tagttacaaa tttaaaccgt
     2821 atcgggttgc cgttttcgtc tttggcgtgc atcgcgccca attcttcttt atattcgacc
     2881 agctcccaat cccgataata atccttcagc tcgccctctt taaatttgaa ggggaacggc
     2941 atcgggcagg ggaaatccgc cgtatccatt gccgatacga ttaagttgta cccgcccgcc
     3001 gccgtatgcg cctgcatatc ggcaatcacg tcgggcacgc gctgcggcat caggaacatc
     3061 agcaccaccg ttgccacaat ataatcaaac tcgccctgaa gggcggcggc gttcaaatca
     3121 tattccagcg tgcggacgtt caaaccctcc gcctctgcca gttccgccac gtttgccaac
     3181 gcggcgggat tgcaatcggc agcggttaca tcaaacccct tcaaacccag gaacagcgcg
     3241 ttgcgcccct gtccgcagcc catatccaac gccctgcccg ccggtacggt atcctgtgcc
     3301 gccgcgaccg cagaatgcgt ggcactcatc ccgtattttt tgtgaaaata gtctgccgcc
     3361 gcgcaataca gcgacaaacg gatttcggca tcgtccgttt tcggtttgac cgaaaacacc
     3421 tgctgcggcg caaacacgca atcgccgctt tccgccgacc aaacctctgc cgccccgtcc
     3481 ggtgcacgga cttcgacatc gccctgcaac acattcaggc agacccattc cccttcctcg
     3541 gacgaataac ccgacaacaa aacttccggc aggttttcca ctttccatac aggcatttgt
     3601 ccgaaacaaa acaactcgcc actttgaccc actatccgct ccttcatatt caaaaataaa
     3661 gttgcacatt atatgcctat ttcaatccgc cgcaatcttt cagacggcac gacgcacaaa
     3721 ccgcttataa tcacgccgga caccacacaa aggcacgata tggacacacc cgtttacctc
     3781 tacacggacg gcgcgtgcaa aggcaatccc ggcgcgggcg gctggggcgt attaatgcgc
     3841 tacggcagcc gcgaaaaaga acttttcggc ggcgaagcgc aaaccaccaa caaccgcatg
     3901 gagctgaccg ccgttatcga agggctgaaa tcgctcaaac gccgctgcac cgtcatcatc
     3961 tgcaccgact cgcaatacgt caaaaacggc atggaaaact ggatacacgg ttggaagcgc
     4021 aacggctgga aaaccgccgc caaacagccc gtcaaaaacg acgacttgtg gcaagaactc
     4081 gacgctctgg tcgggcagca tcaagtcagc tggacttggg ttaaaggaca cgcgggacac
     4141 gcggaaaacg aacgcgccga cgatttggca aaccgtggcg cggcgcagtt ttcttgattg
     4201 ccgcaccgac aaaaatgccg tctgaaaccg ctaatgggct tcagacggca tcgtcctcca
     4261 ccgtcattcc cgcgcaggcg ggaatccaga acgtcgggca acggcaatat tcaaaagccg
     4321 tctgaaaatt taaaagttct agattcccgt tttcacggga atgacgaaaa gttgcggaca
     4381 gcagagg
//
LOCUS       DAWTHD010000127         4365 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWTHD010000127 DAWTHD010000000
VERSION     DAWTHD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.127"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(106..1137)
                     /gene="gap"
                     /locus_tag="WM704_001934"
     CDS             complement(106..1137)
                     /gene="gap"
                     /locus_tag="WM704_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFA9341281.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(1336..2175)
                     /locus_tag="WM704_001935"
     CDS             complement(1336..2175)
                     /locus_tag="WM704_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFA9341282.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            2321..4297
                     /locus_tag="WM704_001936"
     CDS             2321..4297
                     /locus_tag="WM704_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFA9341283.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
BASE COUNT          773 a         1023 c         1473 g         1096 t
ORIGIN      
        1 tggaaatgac gacagccggg taaaatcgcg gtcaaaaatg aaaagtgccg tctgaaactt
       61 gaaaacatcg ggtttcagac ggcattttgt ttgacggttt gtcgcttatt tgagcgggcg
      121 cacttcaagt ccgaacatac ggcgtgcggt gttcagcatt tggcagctga agccccattc
      181 gttgtcatac cacgcgaaca ctttgaccat gttgccgtcg acgactttgg tcagcgttgc
      241 gtcgaagtgg ctggcttcgg tggtgtggtt gaagtccatg gaaaccaatg gcagggtgtt
      301 gtagcccaaa acgcctttga gcgcgcctgc ttccgaggcg gctttcatca gtgcgttgat
      361 ttcttcgact gtggtgtcgc gcgcggcttg gaagctcaaa tctaccaagg atacgttgac
      421 ggtcggcacg cggatggcaa gcccgtcgag tctgcctttc aattcgggca agaccaaacc
      481 gacggctttt gccgcgccgg ttttggtcgg aatcatgttt tccacgccgc tgcgggcgcg
      541 gcgcaggtct ttgtggcgca cgtcggtaac ggtttggtcg ttggtcagtg cgtggatggt
      601 ggtcattgcg cctttgacga tgccgacgct ttcgctcaac actttggcaa ccggcgagag
      661 gcagttggtg gtgcaggaag cgttggaaac gacggtcatg tcggcggtca ggacgctgtc
      721 gttcacgccg tacacgacgg ttgcatcgac atcgtcgccg cccggtgcgg aaatgaggac
      781 ttttttcgcg ccgctttcga ggtggatttt ggctttttct ttgctggtga acgcgccggt
      841 gcattccatg accaaatcga caccgagttc tttccacggc agttcggcag ggttgcgggt
      901 cgagaagaag gggattttgt cgccgttgac gatgaggttg ccgccgtcgt gggatacgtc
      961 ggcttcaaag cgtccgtgta cggtgtcgaa tttggtcaga tgggcgttgg tttcaaggct
     1021 gccgctggcg ttgacggcga cgatttggag ttggtcttga atctgatagt cgtagatggc
     1081 gcgcaaaacc tggcggccga tgcgtccgta gccgttgatg gcgactttga tgcccatggt
     1141 ttgttccttt gttgagggtt gggtagattt tcggggcgga ttatagcaaa tttgtagtgg
     1201 cgtgtaatta atattttatt gaaaacggca cggccggaag ggtgggcggt aagatacgga
     1261 cggcacgggt acggcggacg gagagcttga taaaatgccg tctgaagcgg cttcagacgg
     1321 catgtcgggt aagggtcagg aggcggtatt ctgtgcggct tcctgtttgg ctttgtattg
     1381 tttgaggtat tcgagggcgg cggctttttc gctgtcgctg ccgtatttca tatcgcgttg
     1441 ggcgcggcgc aattcggcgc gttcgcgggc ttcggctatc tgttttgcct gatagccttg
     1501 gcggttgtcg gcggcggcga ggcgatcctg ttgggtttgc gcttttgcca tggctttggc
     1561 gatgaggtcg gcggggttga acgccggttt ttccggtgtg tcgggcgttt gcggacgcgc
     1621 gttgcggacg gcggcttcgc gttcggcaag catagccttg cgttcgtcgg cttcgcgctg
     1681 tttgcgttcg ttgcgtttga ggtagcgcgc acgcgcgtgt tcggcggcgg caaaacggct
     1741 gtcgtcggac aggctgaaac ggcgcgcgcg cggcaggacg gtgtcggcaa cgggctgcat
     1801 atggatgcag tccacggggc agggggcgac gcagagtccg cagccggtgc attcgtcggt
     1861 gatgacggtg tgcataagtt tgcccgcgcc cataatggca tcggcagggc aggcgcggat
     1921 gcaggcggtg cagccgatac aggcggtttc gtctatccgg gcgagtgctt tggcttgggt
     1981 tttggcaggt gcgacaaggg gtttgccgag cagggcggca aggtcgcgga cgacggttgc
     2041 gccgcccggg gcgcagaggt tgtgcgcctc gccccgcagc atcgcgcggg cgtagggcag
     2101 gcagccttca tagccgcatt cgcggcattg ggtttggggg aggagtcggt ctaaatcggc
     2161 ggcggtggcg gtcattgtgc ggtggtgcgg gtcaaaaggg cgtattttat cagaattgta
     2221 tgccgcgccc gtttcggatg gtgcgcggtg ttttgttata atacggcggc gtatgccgtt
     2281 tcagacggca tttttctgta ttttcctgtt cggacggtcc atgaacgaat tttcgcttgc
     2341 ccctattgtg gttgttctgc tggtgtcggt cattacggtg atcctgtgcc gcaagttcaa
     2401 cattccctcc atgctgggct acctgctggt gggctttttg gcggggcccg gtatgctcag
     2461 cctgattccg aaaagccatg cgacggatta tttgggcgaa atcgggattg tgttcctgat
     2521 gttcagcatc ggtttggagt tttcattgcc caagctgagg gcgatgaggc ggctggtgtt
     2581 cggtctgggc ggtttgcagg tcggcgttac gatgctgtcg gtaatgggca tactgatgct
     2641 gacgggcgtg ccgttcaatt gggcgtttgc cgtatcgggc gcgttggcga tgtcgtccac
     2701 ggcgattgtg agccggattt tgtcggaaaa gacggagttg gggcagccgc acggtcagat
     2761 ggcgatgggc gtgctgctga tgcaggacat cgccgtcgtg ccgctgatga ttctgattcc
     2821 cgcgctggct ggcggagggg acggaaatat ttgggcggcc ttgggtttgg cgtttgcaaa
     2881 aatgctgctg acgctggggc tgctgttttt cgtcggcagc aaaattatgt cgcgatggtt
     2941 caggatggtg gcaaaacgca aatcgtccga actctttatg atcaatgtgc tgctggtaac
     3001 cttgggtgtg gcttatctga ctgagctgga aggtttgtct atggcgttgg gcgcattcgt
     3061 tgccggcatg ttgctttcgg aaacggaata ccgtttccaa gtcgaagacg acattcgccc
     3121 gttccgcgat attttgctcg gcttcttctt tatcacggtc ggcatgaagc tggacattca
     3181 ggcattgatc ggcggctggc ggcaggtatt gatgctgttg gcaatgctgc tggtgttgaa
     3241 ggcattggtt gtgtttgcca ttgcattcaa aatgaagcat tcggtcggcg acagcctcaa
     3301 aacggctttg tatctcgcgc aaggcggcga gttcggcttc gtgatgctgg ccattgccgg
     3361 gcagcttgat atggtttcgc cagaattgga acaggcggcg acggcggcgg ttctgctgtc
     3421 gatgattatc gcgcccttcc tcttgggcgg cagcgatgcg ctggtcgggc gcttggtcaa
     3481 gtcaagctgg gacatgaagt cgctcgatct gcacagcatg ttggtggaag cgatgagcaa
     3541 gtctgatcat gtgctgattg tcggcttcgg caggggcggg cagacggtcg gacgcgtcct
     3601 cgcccaagag gatattccgt atttcgcgct cgacttggac atcgcacgcg tgcaggtagc
     3661 cagaagtgca ggcgagccgg tgtcgttcgg cgatgcgaaa cgcagggaag tattggaagc
     3721 cgccggtctg ggacgggcga aaatggtggt ggttacgctc aacaatatgc acgaaacgca
     3781 acacgtttta gacaatgtgc tgtccatgca tcccaatatg cccgtatatg cgcgcgccac
     3841 caatgacgat tatgtgaaaa cgtttaccga tatgggtgcg gaagaagccg tgtcggacac
     3901 caaagaaacc ggactcgtgc tggcaggcta tgcgatgttg ggtaacggcg cgtcgtaccg
     3961 gcacgtctat cagacgatgg caaatatccg ccacagccgt tatgccgcgt tggagggact
     4021 gtttgtcggt agtgatgatg aggcaggatt cggcgaaaat ggcgaaaccg tccgccacgc
     4081 ctttcctttg gctgcagaag catacgccgt cggcaaaaca gtcggcacgc ttccgatggc
     4141 ggcttacggc atcaaactct tgttcgtccg ccgccgcacc ggccggattg aaaacccgga
     4201 tgcctcgttt acattggaag gcggtgacgt gttggtggtc gcaggcaaaa aagaagaaat
     4261 tatctctttt gaaaactgga gcttgcaggg aatataaatg aaatgccgaa ataaggcttg
     4321 cgccattccc ggttatttgg tctaatagcg ttttcgcaaa tcgca
//
LOCUS       DAWTHD010000128         4297 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWTHD010000128 DAWTHD010000000
VERSION     DAWTHD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.128"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..506
                     /gene="mafB"
                     /locus_tag="WM704_001937"
     CDS             <1..506
                     /gene="mafB"
                     /locus_tag="WM704_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFA9341284.1"
                     /translation="LLSESGKTQTVRNTVVKPKPLKAYNLTVADWHTYFVKGDKAETE
                     GVWVHNDCPTKLKPTERYNRQTHYGGSQTDGARAQAARQAGEGKPCPTCGRIQIFGTK
                     TAPSPQHEPPLVKHYYEHGGHSMSNADRAKHARESIKGTQCLTCQRKEGAMMSRYSRE
                     QAKKHGL"
     gene            496..1044
                     /locus_tag="WM704_001938"
     CDS             496..1044
                     /locus_tag="WM704_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFA9341285.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            1341..1694
                     /locus_tag="WM704_001939"
     CDS             1341..1694
                     /locus_tag="WM704_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341286.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1684..2007
                     /locus_tag="WM704_001940"
     CDS             1684..2007
                     /locus_tag="WM704_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFA9341287.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIDGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2066..2293
                     /locus_tag="WM704_001941"
     CDS             2066..2293
                     /locus_tag="WM704_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341288.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2420..2963
                     /locus_tag="WM704_001942"
                     /pseudo
     CDS             2420..2963
                     /locus_tag="WM704_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            3098..3829
                     /locus_tag="WM704_001943"
     CDS             3098..3829
                     /locus_tag="WM704_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9341289.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGCKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVV
                     KPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNECPYGKGNQRYKDAPYHGKNDNSV
                     KSRAPTNGQAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNW
                     KNLHTDQQNALKKAGLVNSKGKIKK"
     gene            3826..4161
                     /locus_tag="WM704_001944"
     CDS             3826..4161
                     /locus_tag="WM704_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341290.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            4221..>4297
                     /locus_tag="WM704_001945"
     CDS             4221..>4297
                     /locus_tag="WM704_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341291.1"
                     /translation="MGNSQTLISNRIHPFYSDGKWIKAE"
BASE COUNT         1394 a          791 c          920 g         1192 t
ORIGIN      
        1 ggctgttatc cgaaagcggc aaaacccaaa ccgtccgcaa caccgttgtc aaaccaaaac
       61 cgctcaaagc ctacaatctg accgttgccg attggcatac ctactttgtc aaaggcgata
      121 aggcggaaac ggaaggggtt tgggttcata atgattgtcc aaccaaacta aaaccgacag
      181 aacgttataa tcgtcaaacg cattatggag gttctcaaac agatggagca agggctcagg
      241 cagcaagaca ggcaggagaa ggtaaacctt gtccaacttg tggtagaatt cagatatttg
      301 gtacgaaaac cgcaccaagt ccgcaacatg aacccccatt agtaaaacat tactatgaac
      361 atggtggtca ctctatgagc aatgctgatc gagcgaaaca tgccagggaa agcataaaag
      421 gaactcaatg tttaacatgt caaaggaaag agggtgcgat gatgtcaaga tattcgagag
      481 aacaggctaa aaagcatgga ctataaccaa actgttttat ctcacttgca aaaattttgg
      541 aaacatcatg atattaaagg atttacttgg actttaggaa gaattgttga agaacttccc
      601 gatttccaag ttttccaagt gattcctaat catgaagatg agccttgggt ttatgtcagc
      661 tctgggatag gtcaattttt agggcaggag ttttttatca tcagcccgtt tgaaacacct
      721 gaacatattg agaccttggc aatgctggca tctgcaagta tgcattatcc tgatcaattt
      781 caattaggga aaaccgtcaa tatcggtaga ccgtgggtcg agcaatcatc cttccggcat
      841 tttctgattt ctctgcctta tccttatggg caagagttgg aatatatgga taatgtccga
      901 tttttctggt tattgccaat tacccagaca gaaagacttt tcttgaatac tcattcggta
      961 gaagaattgg aaacgaaatt tgatgaagcg ggtattgatt atttagatat aaatcgtgcg
     1021 agtactgtct ggcaggcagg ataaaaattc ggcaatcaaa atgccgtctg aaacaagttt
     1081 ggactttcag acggtattct gatgaatgga acaggatact gggaactgag ttcttcaaaa
     1141 atcctatctc cgtcccttat acgtcggtgc tttagtcaaa acggcagacg gatgaaaaat
     1201 tattgcccgt atccttgcca aagatgaagc aagatgaagc caggtaattg aatcggtaca
     1261 agcgcaatgt ccgacttgtt ctgcaaaaca gggagggaga ttaagccaat attcaaaaga
     1321 acagaaaaag aaactgagta atgggaataa aaaatgaaca aataataatt tgtaaaaaat
     1381 ataatacaga aatatatcct gtttccgatg tttcaaaaat cggtgttgct gaaaatgtca
     1441 aacagacagg cctgtatccg attaacgggt taaggcaccg tcccaaaggt gatactaacg
     1501 gttggtatat ctgggccggt gaaaacttct cctatgataa aaattttttc ctgcccttgc
     1561 atacgttcca tttgcaaata tggcgacccg aaatcatccc ctttttgaca cttccgcccg
     1621 gttaccgttt tttaattggg gaaaacggtt atgaagacgt atggtttgat gagttgctat
     1681 taaatgataa ttgacgaaaa atctacttca gatgtactga aacaatatca agaactaggg
     1741 tttgatttga caaaacctat gataatagag ttttttatag atggttctca aaagaatctg
     1801 caatcaatag aaagccaact catttcttat cggcaagatt ttcaaatcag tattgaacaa
     1861 gatgaattcg gagaaatgtg gacttgctat tgttccgtca atatcgtccc ttcccttgaa
     1921 aacattttag ctattgaaac tacgttattt ggtattgcac aaaaaaatga ttgtaattat
     1981 gaaggttttg gttcgtatgg gaactaacaa aaagtgtagg ttgggttgaa aaaaccatat
     2041 tcccattaaa gattagggga tgtctatgga aataaaattc aatactttgg gggttatcct
     2101 gaatggagtc aatcctgaag aaaaattcat aaaaattatt gatgaccagg aaaataccgg
     2161 tggatttctg atactgttat cttcaaatga caaattctca tcatttgaca gttacgatga
     2221 ctgggtcgaa aacttagaaa tattaaaaga atacctgcaa gaatctcatt ggataataaa
     2281 gtgggtcgga tagtcggcaa acgtagtccg tttgaaacgc ttttactttt cagacggact
     2341 ttctaccaat gggaacaagg cgttttgtct caatccggtt tatgattatt cgatacagtg
     2401 atgggaagct aagaaaaata tgattaatca tattgaaaaa tatataggca ctatttctca
     2461 tggctcgaaa agcgattcgg gaagtcagta caaattaaat atcgcagcta ttccctcttc
     2521 ccctaacagg gatttaaaaa cttatattac tttagggtta agcaaacatg atctgaatta
     2581 taaaagcaga tttgaaatac tttttgtatg ttctttaaaa tatgatgaaa atcagatttt
     2641 cccattttta agatggttag cagaaaccat cattgagaat aaaaaaaatc cttcttcggg
     2701 ggcaggttgt ttatttgcct agaagcattg ttaattcaac aaaaatggac gcactgtatg
     2761 tttccgcccc attttatttc gacgatgatt tccaagtctg ttatggcgaa cactacaata
     2821 ttgttttccc tttgcttgtc cccttgtata aacaggaagc cgaattggtg gaaaaaaagg
     2881 gttggaatgc ttttgagcag ttcttgttgg ataatgaagt tggcaacctt tcggatatga
     2941 ataggaaacc gtttgtttgg taagggcttt cttaaatttg attaaaggtc gtctgaaatt
     3001 aagcttgttt ttcagacggt ctttccttca tttgaaacag gatattgaaa actaagttct
     3061 tcaaaaatcc tacacctgct ccttccacgg cagcaccttg gtcaaaacgg cagacggcta
     3121 caaagccatt gcccgtatcc gagccggcga gagcgtcctc tccaaggacg aggcaagcgg
     3181 ggtaacggga tgcaaacctg ttaccgcccg atacggcaat ccgtatcggg aaaccgttta
     3241 cattgaagtt tcagacggca tcggcaacag ccaaaccctg atttccaacc gcatccactc
     3301 gttttattcg ggcggcaaat ggattaaggc ggaagattta aaagcgggaa gcagactgtt
     3361 atccgaaagc ggcagaaccc aaaccgtccg caaaaccgtt gtcaaaccaa aaccgctcaa
     3421 agcctacaat ctgaccgttg ccgactggca tacttacttc gtcaagggca atcgggcgga
     3481 aacggaaggg gtttgggttc ataatgagtg tccgtatgga aaaggcaacc aacgatataa
     3541 agatgctcct tatcatggta aaaatgataa ttctgtgaaa agtagagcgc caacaaatgg
     3601 gcaagctgtc cttgataact ctgttcaagt taaatcgaca tcttctcaaa gagttggagt
     3661 tgataaaacc aataatgaaa tcgttgtatt aaaccaaact agaattttta atgacggttc
     3721 tgctgagtat catgggcatg taaggaactg gaaaaattta catactgatc aacaaaatgc
     3781 tttaaagaaa gcaggattgg ttaattcaaa aggaaaaatc aaaaaatgat ggctgataag
     3841 gaacaagtag aaaagatgat ttattctgaa aataaagatt gtgttattga tggtatgctc
     3901 ggtatgacct tcagctcgga ggaggatgaa atcccttgga tttccgagaa attgacggag
     3961 ttgtcgaagc ataaagactt ggatattgcc agattgtcat taacctgttt cggacatctc
     4021 gccaggatgc atgaaaatat cggcgattgc gataaagtga ttgctttatt gcgctcaaaa
     4081 caaggtaatc cggattttca aggttttgcc gaagatgcgt tagacgatat ttccttcttt
     4141 atatttaaaa agcgtccgta agcgcggatt aaacccggaa atccgatgcg tctgaaagta
     4201 cattccggct ttcagacggc atcggtaaca gccaaaccct gatttccaac cgcatccatc
     4261 cgttttattc ggacggcaaa tggattaaag cggaaga
//
LOCUS       DAWTHD010000129         4153 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWTHD010000129 DAWTHD010000000
VERSION     DAWTHD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.129"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            37..228
                     /gene="mobC"
                     /locus_tag="WM704_001946"
     CDS             37..228
                     /gene="mobC"
                     /locus_tag="WM704_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFA9341292.1"
                     /translation="MRVLAGMGNNLNQIAKALNTAAKVGTLGNVEALKATTELAALER
                     SLNSLRDFLAKEKNGWQSQ"
     gene            213..842
                     /locus_tag="WM704_001947"
     CDS             213..842
                     /locus_tag="WM704_001947"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFA9341293.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQS
                     MTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            926..1513
                     /locus_tag="WM704_001948"
     CDS             926..1513
                     /locus_tag="WM704_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic plasmid protein B"
                     /protein_id="HFA9341294.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMMKALTHLGERLAELEQE
                     NTSLAQQLASLAAELERQSEIQQRQSEILNQLAKR"
     gene            1545..1814
                     /locus_tag="WM704_001949"
     CDS             1545..1814
                     /locus_tag="WM704_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic plasmid protein A"
                     /protein_id="HFA9341295.1"
                     /translation="MGNEPMITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYP
                     SNIAKVLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1792..2070)
                     /locus_tag="WM704_001950"
     CDS             complement(1792..2070)
                     /locus_tag="WM704_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFA9341296.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2070..2255)
                     /locus_tag="WM704_001951"
     CDS             complement(2070..2255)
                     /locus_tag="WM704_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFA9341297.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2258..2503)
                     /locus_tag="WM704_001952"
     CDS             complement(2258..2503)
                     /locus_tag="WM704_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341298.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2500..2742)
                     /locus_tag="WM704_001953"
     CDS             complement(2500..2742)
                     /locus_tag="WM704_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFA9341299.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2746..3528)
                     /locus_tag="WM704_001954"
     CDS             complement(2746..3528)
                     /locus_tag="WM704_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFA9341300.1"
                     /translation="MLVRSYAAAIKRRYIQVNPPHLRVFMLFDLDYEGAGLAWEDNNL
                     PMPAWAAINRENGGAHLAYALSAPVLTAEYGGRQKALRYLAALEAAYKAKLRGDVGFV
                     SLITKNPEHPHWLTLRGVPDAIRGYDLEYLADFVDLDKFKPYIGRSNVEAVGLSRNCT
                     VFNLVSRWAHKNVLAFKQQGYTVQGWLKEVHYQCMRVNGDFPVPMWEKEVKCISKSIA
                     NWVWYKFDIAASNRRFSELQAHRNSLRKTTINAGRTKIITEL"
     gene            complement(3698..4096)
                     /locus_tag="WM704_001955"
     CDS             complement(3698..4096)
                     /locus_tag="WM704_001955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341301.1"
                     /translation="MGLLSGQSLKLGRADSDDELLCSLFGGTRTCQKGKIPLFLTAFC
                     SKKRHFITAFRRYSMKNSGKNTHAALSELVSCFPQNAALLAGLGRAAALQALPLRLLA
                     SLNCGLENFEIRTPIGLLSDYGYFDIVVSS"
BASE COUNT         1127 a         1026 c         1112 g          888 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag agcatcacag acggcttaga agccctagcc
      781 ctatcaggca agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt
      841 gaaatatcac gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc
      901 aacatccgac tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga
      961 gcagacatca cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac
     1021 gttacggaaa aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat
     1081 aaacgctcgg cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct
     1141 aggtatatcg gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt
     1201 caagaacgag ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct
     1261 gttggaaacc aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc
     1321 aacgcgggta tgcgtagcga ccgagaagcg aatgatgaaa gccttaacgc acttgggcga
     1381 aaggttggca gagctagagc aggaaaacac gagtttagcg cagcagctag cgagcttggc
     1441 agccgagtta gagcggcaga gcgaaataca gcaacggcag agcgaaatct tgaatcaact
     1501 agccaaacga taagccaacg acacaaacga acccaaagca ggggatgggg aatgagccga
     1561 tgattaccga gaacgaacgc gacaggcgaa cagccgcatg gctgatagag acctacgggg
     1621 cagaagccgt agcggaagca gaaacccgca ttgcgggcgc gagaaagccc tatccgagca
     1681 atatcgccaa agtattgggg gctagcctac ccgaagccct aaaacgcaca gaaaacgccg
     1741 cagcgcgcca aaaactggca gggctgcggc ggatgttgga cggtaaggca gttaagactt
     1801 cacaagactt gtgaaatcag accactgctc aatgcggaac gcccgaatat cgcggacaga
     1861 agacggaaac caaggcagag cttttagctc gttgatggct gaaaacaggt tagccatatc
     1921 ttcgttttgg caggtgtaca aacttccctg aatgcgggta aagccgaatt tccgcagggt
     1981 gtatccaata tccgcgtagg cttgggaaat gcctttcgga tggttttgag cggtatcggc
     2041 aaccaccaaa tcaaaagaaa tcgcgtacat taggcagcct ttgggtcttg aacgatgtgg
     2101 gacagtttgt attgcgtgtg ttcgccgctt tccggcagtt caatatttac gagccaatcg
     2161 ccgtccggaa gctgttcggc aggcgtgccg acgatgtaca tcacgccgta atcgccaaag
     2221 gtgcggattg aaccggtggg gatagtggtt tgcatggtta gttctcctgt tgggtttggt
     2281 tttcaggctg gtttgtcatg tacaaaaccc gttgggcagc tttttcggct gttgtgcgtt
     2341 tgatgccgtc aggcagaaaa attctccatt ttttggctag aactatatct gcaagatcat
     2401 caaccgttgc gcttttcaaa gcaggatttg cgttaatttc cgaatacaga aaatctttga
     2461 atgccgagta cagagcatcg cggtttattg cgtatcttgt catgctgttt cgctttggtt
     2521 gtcagtttca gacggcatag gcttgccgcg cctgtaccaa gtggcgcggg aaatgccgaa
     2581 gtgttcccac ggtttatcgc ggctgataga gttttcggct aggtagtcgg cgcgggattg
     2641 cgaccatgcc cgaaaaacgg ttcttgtgga acagccgaag cgttttgcca gttctttagc
     2701 cgtaacgtct cgcttggtgg ttttcagttt tggataggcc atatttcaaa gctccgtgat
     2761 gatttttgtt ctgcctgcat tgatggttgt tttccgcaaa ctattccgat gagcctgcaa
     2821 ttccgaaaac cgtcgattgc tggctgcaat atcaaacttg taccaaaccc agttagcgat
     2881 tgatttggag atacatttca cttctttttc ccacatcggg acagggaaat ccccatttac
     2941 ccgcatacac tggtaatgca cttctttcag ccagccttgc accgtgtagc cctgctgttt
     3001 gaacgccaac acgtttttgt gcgcccaacg gctcacaagg ttaaacaccg tgcaatttct
     3061 gcttaatccg accgcttcca cgttagagcg accgatatag ggcttaaact tgtctaaatc
     3121 cacgaaatcc gcaagatact ccaaatcgta gcccctgatt gcgtcaggaa cgccgcgcag
     3181 cgtcagccaa tgcggatgtt cgggattttt cgtaatcagc gatacaaagc ccacatcacc
     3241 gcgcaatttc gccttatatg ctgcttcaag tgcagcaaga tagcgcaggg ctttttgtct
     3301 cccaccgtat tccgccgtca gcacaggcgc ggaaagcgca taggcaaggt gtgcgccgcc
     3361 gttttccctg ttgattgccg cccaagcagg cataggcaga ttattgtctt cccaagccaa
     3421 ccccgcccct tcgtaatcca agtcaaagag cataaacaca cgcagatgcg gcggattgac
     3481 ttggatgtag cggcgtttga tggcggcagc gtaagagcgc accagcatag gcgcttcttt
     3541 gaaatctttg cagtatggct tgtgtgggat acgttcttgc aagaagaggt cgggttgggt
     3601 gtataattgg ctcatgttgt atctcgaaac ccccgtgcag attggcgttt ggcgggggtt
     3661 ttgctttgtc taagatttgc agattgtatg cttgttttta agatgataca actatgtcaa
     3721 aataaccata atcagataac agcccgatag gggttcttat ttcaaaattt tccaatccgc
     3781 aatttagcga agccagcagg cgaagcggta aagcttggag cgcagcagcg cgacctaagc
     3841 cggccagcag ggcggcgttt tgggggaaac atgaaaccag ttccgacagg gcggcgtgcg
     3901 tgttcttccc tgagttcttc atggagtatc ggcgaaatgc cgtgatgaaa tgccgttttt
     3961 ttgagcagaa agcagtcaaa aacaggggta ttttgccctt ttgacaggtt cgagtgccgc
     4021 cgaaaagcga acaaagcaac tcatcatccg agtcagcccg accgagtttg agactttgac
     4081 ccgacagaag acccatccga atttagcccg ctacattcgg gagcgggttt tggaagatgg
     4141 caaagcatcc gac
//
LOCUS       DAWTHD010000130         4021 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWTHD010000130 DAWTHD010000000
VERSION     DAWTHD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.130"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            361..1155
                     /locus_tag="WM704_001956"
     CDS             361..1155
                     /locus_tag="WM704_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFA9341302.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(1204..1308)
                     /locus_tag="WM704_001957"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="WM704_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1380..3983
                     /gene="pepN"
                     /locus_tag="WM704_001958"
     CDS             1380..3983
                     /gene="pepN"
                     /locus_tag="WM704_001958"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFA9341303.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFAFVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT          962 a         1113 c         1106 g          840 t
ORIGIN      
        1 aagcaataat ctaaatcagg catttttatt cccctgtttt aaaaaatatt tggcaaggtg
       61 tgaaaaaagg cgtacattcc gctacacaga attacagata cagcggagca atgccgtctg
      121 aaaggatttt ccggtcagtc ttgcggttgg tcggggtttc atcggatacg gtgaaacgaa
      181 agtttgccgg cgcagggttg agctacgcgg gtaaagccgc aggcgaaagc ctgtattgtt
      241 tgtgaagcgt aaatctctga tttgaggtat tggggcaatc ctgtgggggt tgcctctttt
      301 tttatccgcc ttttaatgac acaataggcg caagtctgtt tttgaatgca aggtactgcc
      361 atgaatacga ttttggcttt cgatattgaa accgtacccg atgtgcaggg tatccgtacg
      421 ttgtatgact tgccgtcttc cctgccggat gacgaagtgg tgctgttcgc gcagcagaaa
      481 cgccgcgcac agaccggcgg cgattttatg cagcaccacc tccatcaggt tgtggcggtt
      541 tcctgctgta tgcgctgggg gcaggacaag gttcatgtcg gcaccatcgg cgagatggac
      601 gatggcgaag aagtcgtgat tgccaagttt ttcgaattgg ttgaaaaaca tacgccgcaa
      661 ctggtcagct ggaacggcgg cggtttcgac ctgcccgtac tgcattaccg ctccctgata
      721 tacggcatca acgccgcgcg ctattgggat atgggcgacg gcgatttcgg cgacagccgc
      781 gatttcaagt ggaacaacta catcagccgt tatcaccaac gccactgcga tttgatggat
      841 ttgctcgcgc tttaccagcc gagggcgaat gtgccgctgg acgatatggc gaaactgtgc
      901 ggctttccgg gcaagctggg tatggacggc agcaaggtgt gggaggcgtt ccacgcgggc
      961 aggctgaagg aaatccgcaa ttattgcgaa accgatgccg tgaatacgta tttgatgtat
     1021 ctgcgtttct gtctggtcag cggcagattc gacgcggacg aatacgaaat ggaaatcaag
     1081 cggatcagaa actatctctc cgcccaaaca gaagacaaac cgcattgggc agaatttgtc
     1141 caagcgtgga aatagaaacc tgctgtccga aggcggtacg cttgtgccgc cgtttcggtt
     1201 tcagacggcg tatggtggat taaattcaaa ccggtacagc cttgcctctc cttgccttac
     1261 tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc gctttaaaag
     1321 ctgtatccgt gccgtctgaa gccttttcca gtcaaaccaa acaatattga aagaaaaaaa
     1381 tgagcaaaac cgtccgttat ctgaaagatt accaaacgcc tgcctaccgc attcttgaaa
     1441 ccgaactgca tttcgacatt gccgaaccgc aaaccgtcgt gaagtcgcgt ttgacggtcg
     1501 agccgcagag ggcgggcgag ccgctggtgt tggacggttc ggcaaaactc ttgtccgtca
     1561 aaatcaacgg cgcggcggcg gattatgtgt tggaaggcga gacgctgacg attgcagacg
     1621 taccgtccga acgcttcacc gtcgaagtgg aaaccgaaat cctgccggcg gaaaacaaat
     1681 cgctgatggg gctgtatgct tccggcggca atctgtttac ccagtgcgag ccggagggct
     1741 tccgcaaaat cacgttctac atcgaccgtc cggatgtgat gtccaagttc acgaccacca
     1801 tcgtcgcgga caaaaaacgc tatcccgttt tgctttccaa cggcaacaaa atcgacggcg
     1861 gcgagttttc agacggccgc cattgggtga aatgggaaga cccgtttgcc aaaccgagtt
     1921 atctgtttgc tttggtcgcg ggcgatttgg cggtaacgga agaccgtttc accaccatga
     1981 gcggcagaaa cgtcaaaatc gagttttaca ccaccgaagc ggacaagccc aaggtcggct
     2041 ttgccgtgga atcgttgaaa aacgcgatga agtgggacga aacgcgcttc gggttggaat
     2101 atgacttgga tattttcatg gtcgtcgccg taggcgattt caatatgggc gcgatggaaa
     2161 acaagggttt gaacattttt aacaccaagt tcgtcctcgc cgacagccgc accgccaccg
     2221 ataccgattt cgaaggcatt gaatccgtgg tcggacacga atatttccac aactggacgg
     2281 gcaaccgcgt aacctgccgc gactggttcc agctttcgct gaaggaaggg ctgaccgtgt
     2341 tccgcgacca agagttttcc ggcgaccgcg ccggccgcgc cgtgcgccgc atcgagaaca
     2401 tccgcctgct gcgccagaac cagttccccg aagacgcagg cccgaccgcc catccggtgc
     2461 gccccgtcag ctatgaggag atgaacaatt tctacaccat gaccgtttat gaaaaaggcg
     2521 cggaagtggt gcggatgtat cataccctgc tcggcgaaga gggcttccaa aaaggcatga
     2581 agctatattt ccaacgccac gacggacagg cagtgacctg cgacgatttc cgcgcggcga
     2641 tggcggatgc gaacggcatc aatctcgacc agttcgcctt gtggtacagc caggcgggca
     2701 cgcccgtttt ggaagccgaa ggccgtctga aaaacaatgt tttcgagtta accattaaac
     2761 aaaccgtgcc gcccacgccc gatatggcgg acaaacagcc gatgatgatt cccgtcaaag
     2821 tcgggcttct gaaccgcaac ggcgaagcgg cggcattcga ttatcagggc aaacgcgcaa
     2881 ccgaagccgt attgctgctg accgaagccg aacaggcctt cccgctcgaa ggtgtaaccg
     2941 aagccgtcgt tccctcgctg ctgcgcgggt tcagcgcgcc ggtgtatctg aactatccgt
     3001 acagcgacga cgacctgctg ctcctgctcg cccacgacag cgacgctttc acgtgctggg
     3061 aagccgccca aacgctctac cgtcgcgccg tcgccgccaa ccttgccgcg ctttcagacg
     3121 gcatcgggtt gccgaaacac gaaaaactgc ttgccgccgt cgaaaaagtc atttcagacg
     3181 acctcttgga caacgccttc aaagccctgc ttttgggcgt gccgtccgaa gccgaactgt
     3241 gggacggcac ggaaaacatc gacccgctgc gctaccatca ggcgcgcgaa gccttgttgg
     3301 atacgcttgc cgtccgcttc ctgccgaaat ggcacgaatt ggaccgtcag gcggcgaagc
     3361 aggaaaacca aagttacgaa tacagccccg aaaccgccga ctggcgcacg ctgcgcaacg
     3421 tctgccgcgc cttcgtcctg cgcgccgacc ccgcgcacat cgaaactgtt gccgaaaaat
     3481 acggcgaaat ggcgcaaaac atgacccacg aatggggcat cctgtccgcc gtcaacggca
     3541 acgaaagcga tacgcgcaac tgcctgctgg cgcagtttgc cgacaagttt tcagacgacg
     3601 cgctggtgat ggacaaatat ttcgccttcg tcggctcaag ccgccgcagc gacaccctgc
     3661 aacaggttca aaccgccttg cagcatccga aattcagtct cgaaaacccc aacaaagccc
     3721 gttcgctcat cggcagcttc agccgcaacg tcccgcattt tcacgcacaa gacggcagcg
     3781 gctaccgctt catcgccgac aaagtcatcg aaatcgaccg cttcaacccg caggtcgccg
     3841 cccgcctggt gcaggcgttc aacctctgca acaagctcga gccgcaccgc aaaaacttgg
     3901 tgaaacaaga attgcagtgc attcgggcgc aggaaggatt gtcgaaagac gtgggcgaaa
     3961 tcgtcggcaa gattttgggt tgaggcagtc aaacagaaaa aacaagggtc tgtaccagat
     4021 t
//
LOCUS       DAWTHD010000131         3909 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWTHD010000131 DAWTHD010000000
VERSION     DAWTHD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.131"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            26..1402
                     /gene="nhaC"
                     /locus_tag="WM704_001959"
     CDS             26..1402
                     /gene="nhaC"
                     /locus_tag="WM704_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFA9341304.1"
                     /translation="MFAFKSLLDMPRGEALAVVALIAAMGYTIISLEWLPHMSIIAAI
                     VVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYYG
                     FGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIVS
                     GVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAAQ
                     DLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLHS
                     TPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFALG
                     VIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGLH
                     SRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFGW
                     TGLTLSKK"
     gene            complement(1545..2369)
                     /locus_tag="WM704_001960"
     CDS             complement(1545..2369)
                     /locus_tag="WM704_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFA9341305.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2371..2886)
                     /gene="ybeY"
                     /locus_tag="WM704_001961"
     CDS             complement(2371..2886)
                     /gene="ybeY"
                     /locus_tag="WM704_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFA9341306.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            3005..>3909
                     /gene="hemC"
                     /locus_tag="WM704_001962"
     CDS             3005..>3909
                     /gene="hemC"
                     /locus_tag="WM704_001962"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFA9341307.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQE"
BASE COUNT          765 a         1008 c         1133 g         1003 t
ORIGIN      
        1 tttatatcaa atcgggcaaa gaatcatgtt tgctttcaaa tccttactcg atatgccgcg
       61 cggtgaggca cttgccgtcg tcgctctgat tgccgcaatg ggctatacca tcatttcatt
      121 ggagtggctg ccgcatatgt ccattattgc cgccatcgtc gtgctgattt tgtacggctt
      181 ggcgcgcggt ttgaaataca acgatatgca ggcagggatg ataggcgcgt tgaatcaggg
      241 tatgggcgcg gtttacctgt ttttcttcat cgggctgatg gtcagcgcgc tgatgatgag
      301 cggcgcgatt ccgacgctga tgtattacgg tttcgggctg atttccccga cttattttta
      361 tttttccgcc ttcgcgctgt gttccgtcat cggcgtgtcc atcggcagca gcctgaccgc
      421 ctgcgccact gtcggcgttg cctttatggg gatggcggcg gcgtttcagg ccgatatggc
      481 gatgacggcg ggcgcgattg tttccggtgt gtttttcggc gataaaatgt ccccgctttc
      541 cgacaccacg ggcatttccg cgtccatcgt cggtatcgac ctgtttgaac acatcaaaaa
      601 catgatgtac accaccatcc ccgcgtggct tatcagcgcg gcactgatgc tttggcttct
      661 tcccagcgtc gccgcgcagg atttgaacag cgtcgaatcc ttccgcagcc agcttgaagc
      721 cacgggattg gtgcacggct attcgctgat tccgtttgca ctgttggtcg ttttggcatt
      781 gatgcgcgtc aatgccgtgg tcgccatgct ctttaccgtc attgccgccg ttgccgtaac
      841 gtatctgcac agcacgcccg atctgcgtca gctcggcgcg tggttttatg gcggctacaa
      901 actcgaaggc gaagcgttta aagacattgc caaactgatt tcgcgcggcg gcttggagag
      961 tatgttcttt acgcagacca tcgttatcct cggtatgagt ttgggcgggc tgctgtttgc
     1021 gctcggtgtg attccttccc tgctggaggc cgtccgtacc ttcttgacga atgccggacg
     1081 cgcgacgttc agcgttgcca tgacttcggt cggggtcaat ttcctgattg gagagcaata
     1141 tttgagcatc ctgctttcgg gagaaacgtt caaacccgtt tacgacaaac tcggcctgca
     1201 ttcgcgcaac ctgtcgcgga ctctggaaga tgcggggacg gtgattaacc cgctcgtgcc
     1261 gtggagcgtg tgcggcgtat ttatcagcca cgcccttggc gtacccgttt gggaatatct
     1321 gccttatgcc tttttctgct atttgagttt ggctttaacc ctgttattcg gctggacggg
     1381 gctgactttg agcaaaaaat aagcggataa gcgaaatgcc gtctgaacct gttttccggt
     1441 ttcagacggc atttttatgt ttggcggatg gggcggattg aaacagaaaa cgaccgtacc
     1501 gtcatcctaa actgtgcaga aacggcggtg cgggcaggct ctgcttactt cacgcgggtc
     1561 gccatcagcg tgtgcaggcg gcggttgtcg gcgcgggcga cggtgaactg caaaccgccg
     1621 ataaggactt tttcgccgcg cacgggcagg tgtcccaatt cctgaatgac caagccgccg
     1681 atggtgtcgg cttcttcgct gccgtattcc gtaccgaaaa aggcgttgat gtcttcgatt
     1741 tcggtagccg cgtggatgcg ccagcgttcg gcggaaacgg agtggatgtt gtcggcgctt
     1801 tcgtcttcgt caaactcgtc ttcgatgtca ccgacgattt gctcgatgat gtcttcaaag
     1861 gtgaccaaac ccgacgtgcc gccgtattcg tcgatgacga ttgccatatg gttgcgctgt
     1921 tcgcggaact cttttaaaag ggcggtcaaa gatttgcctt cgggcacgaa aacggcaggg
     1981 cgcaagacgg atttcaggtg gaactgctcg gggttgaaca tatatttgag caggtctttg
     2041 gcgtgcaaaa tgcccaaaac ttcgtctttg tcttcgccga tgacggggaa gcgcgaatgg
     2101 gcggtatcga tgacgtaggc ggtgatgcgt tcgatgctgt cgttttcttt caatacgttc
     2161 atgcggctgc gcgtaatcat cgcatcgcgc acttccagct cggcaaagtc caatactttt
     2221 tccagccggg tcagtgtgtc ggcatcaaaa acttcctgtt cgtgcgcctg ccgaagcagg
     2281 tttaatacgt cttcggcgga atcgggttcg cgggcgagtc gggcaatcag gcgttcaaaa
     2341 aaatttgttt tcggttgtgc gccgtccatt ttaatgtccg tcctctcggt aggggttggg
     2401 atagcctgcc gcccgcatca ggcggatttc ttcggcttcc attatttcgg cctcatcgtc
     2461 tttgatgtgg tcgtatccca tcaggtgcag cgtgccgtgt atggttaagt gggcaaaatg
     2521 ccgttcaggc gttttgccct gttcggcggc ttcttttaaa acgacttgcg ggcagataat
     2581 caaatcgccg tacagtcttt ccgaaacttg gcagggcagg atttcgcctt cgttgagcgc
     2641 gaaactcaat acattggtgg cataatcttt gccccggtag tcgcggttgt aggcgcgggc
     2701 ttcttcttcg tccaaaagaa tcaaaccgat gtcggcgcgg aggtattcgt ttttcaaggc
     2761 agaccacgcc cagcggtaaa aatcgcgttc ggcggggatg ccggcggcgg aagaggcgtt
     2821 ttcaaagttc aaatggaaac gttgccgctg caacgttaag aaagggtatt ttttggcgcg
     2881 tttcattgtg gcgggtttcg tgttttgtgg gcgtaaatat aacatagacc tgacggtgcc
     2941 gtctgaagaa acgttcaaaa tatgatagac tccacgccgt ttccattctt tgaacgcatt
     3001 gaacatgaac ccgaaaaaac ttgttatcgc cagccgcgaa agcctgcttg ccatgtggca
     3061 ggcgaaacat atccaaggcc gtctgaaggc gctgtatccc gattgcgaag tcgaaatttt
     3121 gggcatgacc acgcgcggcg accggatttt ggacagaact ttgtcaaaag tcggcgggaa
     3181 aggcttgttt gtcaaagagt tggaacaatc ccttcaagac gggcgcgccg atttggcggt
     3241 gcattcgatt aaggacgtgc cgatggattt gcccgaaggc ttcgcccttg ccgccatcag
     3301 cgaacgcgcc aatccgtttg acgcgtttgt gtccaaccga tacgcgcgtt tggaagaaat
     3361 gcccgaaggc gcggttgtcg gcacatccag cctgcgccgt gaagcccagt tgcgcgcgcg
     3421 ctatccgcat ttgcttatca aacctttgcg cggcaatgtg caaacccgtt tgtccaaact
     3481 cgacaacggc gaatacgatg caattatctt ggctgccgcc gggttgcagc gtctggaatt
     3541 ggatgaacgc atccgcatga ttttgtcgga atccgacagc ctgcctgccg ccggacaagg
     3601 cgcattgggt attgaaattg ccacgcatcg cgaagatttg tacgaagtct tgaagccgtt
     3661 aaaccacgat accacacacg cctgcgttac cgccgaacgc gctttggcgc gcgctttggg
     3721 cggaagctgc caagtgccgc tggccgcgta ttgcactgaa gaaaacgggc tgctgatctt
     3781 gcgcggattg gtcgggcacc ccgacggatc gattgtgttg caggcggacg cgcaagcccc
     3841 tgccggatat gccgatgcgc ttggacgtgc ggttgccaaa aaactggcgg acgacggtgc
     3901 gcaggaatt
//
LOCUS       DAWTHD010000132         3892 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWTHD010000132 DAWTHD010000000
VERSION     DAWTHD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.132"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            25..1077
                     /locus_tag="WM704_001963"
     CDS             25..1077
                     /locus_tag="WM704_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9341308.1"
                     /translation="MPPPLPPVPGGGVAEPHVPVSIPTATPLPAGEVTLSSDNGNIEN
                     INTAGAGSASDAPSRSRRSLDAAPQNTSGISIRQREVEKDYFGYKSKETSFIFKTPGG
                     AQYALSSYADPITVSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDY
                     MKFGAWIGPNGEIDLFAGGFPVGKTPPPAFSYGSSTPETALSKGKITYQVWGIRVRNG
                     QFVTSSYTPPKSGSYYGTLANTPVLSFITANFNSNTLAGKILGNSDYGPDVDIQNATI
                     TGPTFSGDATSGGKSGKLEGKFFGKFASTRSSEVSIGGKITFDGDRSLDTVFGGVSYE
                     KKLDDTSQDTNHLTKQ"
     gene            1105..3534
                     /gene="hupB"
                     /locus_tag="WM704_001964"
     CDS             1105..3534
                     /gene="hupB"
                     /locus_tag="WM704_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFA9341309.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLTNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         1029 a         1230 c          917 g          716 t
ORIGIN      
        1 acaaagccct gcccttactg ccgcttgccg ccgcccttgc cgcctgtgcc gggggggggg
       61 gtagccgaac cgcacgtccc cgtgtccatc cccaccgcca cgccgctgcc cgccggcgag
      121 gtaacgttat caagcgataa cggcaatatc gaaaacatca acaccgccgg cgccggaagc
      181 gcatccgacg cgccgagccg cagcagacgc tcgctcgatg ccgccccgca aaacacatcc
      241 ggcatttcca ttcggcagcg ggaagtagaa aaagactatt tcggctataa atcaaaagaa
      301 acatcattca tcttcaaaac acccggcggc gcgcaatacg ccctttcatc ctacgccgac
      361 cccataaccg tcagctactc ctcccccgat ttcaaaattc ccgaccgcca cgcagggcag
      421 cggctcgccg acggcagccg catctttatc tgctgcagcg actccggggc tacttcgtat
      481 gcggaaatta ccaaacaaga ttatatgaaa ttcggggcgt ggataggtcc caacggcgaa
      541 atcgacctct tcgccggcgg cttccccgtc ggcaaaacgc cgcctcctgc gttctcctac
      601 ggcagcagca cgcccgaaac cgcattgtca aagggtaaaa tcacttatca ggtttggggc
      661 atccgcgtca gaaacgggca atttgttact tcctcttata cgccgccgaa aagcggttct
      721 tattatggga cacttgccaa tacccccgtc ctttccttta tcaccgccaa tttcaacagc
      781 aacacactgg caggcaaaat cctcggcaac agcgattacg ggccggatgt ggatattcaa
      841 aacgcaacga ttaccggtcc gaccttttcc ggcgatgcca cgtccggcgg gaaaagcggc
      901 aaattggaag gcaagttttt tggcaagttc gccagcaccc gcagcagcga agtcagcata
      961 ggcggcaaaa tcacctttga cggcgaccgc tccctcgata ccgtattcgg cggtgtgagt
     1021 tatgaaaaaa aacttgatga caccagtcaa gataccaacc accttactaa acaataaacc
     1081 acctcatccc tgcaaaggtt tcttatgccc attcccttca aacccgtatt ggctgccgcc
     1141 gccatcgccc aagcgtttcc cgcctttgcg gcagaccccg cgccgcagtc cgcccaaacg
     1201 ctgaacgaaa tcaccgttac cggcacgcac aaaacccaaa aactcggcga agaaaaaatc
     1261 cgccgcaaaa ccttagacaa gctcttgacc aacgacgaac acgacctggt gcgctacgac
     1321 cccggcattt ccgtcgtcga aggcggcagg gcgggttcta acggctttac catacgcggc
     1381 gtggacaaag accgcgtcgc catcaacgtt gacgggctgg cgcaggcgga aagccgctct
     1441 tccgaagcct tccaagaatt gttcggcgcg tacggcaact tcaacgccaa ccgcaacact
     1501 tccgagccgg aaaacttttc cgaagtaacc atcaccaaag gcgcggactc gctcaaatcc
     1561 ggcagcggcg cattgggcgg cgcagtcaat taccaaacca aatccgcaag cgactatgtt
     1621 tccgaagaca agccctacca tttggggata aagggcggca gcgtcggcaa aaacagccaa
     1681 aaattcagca gcattaccgc cgccggcagg ctctttggtt tggatgcctt attggtttat
     1741 acccgccgct tcggcaaaga aaccaaaaac cgctcgaccg agggcgatgt cgaaattaaa
     1801 aacgacggat atgtctttga cccggccaac ccctcgccca gcagatacct tacctacaaa
     1861 gccacagggg ttgctcgctc ccaacccgac ccgcaagaat gggtaaacaa aagcaccctg
     1921 ttcaagctgg gctacaattt caacgaccgc aaccgcatcg gctggatttt tgaagactcg
     1981 cgcaccgacc gttttaccaa cgagctgtct aatttgtgga cgggtacgac cacgtctgcc
     2041 gcaacgggcg actaccgcca ccgccaagac gtgagctacc gccgccgcac cggcgtcgaa
     2101 tacaaaaacg aattggaaca cggcccgtgg gacagcctca agctgcgcta cgacaagcag
     2161 cgcatcgata tgaacacttg gacttgggac atcccgaaaa attacgatac aaacggcatc
     2221 aacggcgagg tttaccattc gttccggcat atccgccaaa acaccgcgca atggactgcc
     2281 gattttgaaa aacaactcga cttttccaaa gccgtttggg cggcgcaata cggcttaggc
     2341 ggcggcagag gggacaatgc caactcggat tacagttatt tcgcaaaact gtacgacccc
     2401 aaaatcctcg cttccaacca agccaaaatc acgatgctga tcgaaaaccg gtcgaaatat
     2461 aaatttgcct attggaacaa tgtgtttcac ttgggcggca acgaccgctt ccgcctgaat
     2521 gcgggcatac gctacgacaa aaacagcagc agcgcgaaag acgatccgaa atacaccacc
     2581 gccatccggg ggcagattcc ccatttgggt tcggaacgcg cgcacgcggg cttcagctac
     2641 ggcacagggt tcgactggcg gtttaccaag catctgcact tgttggcaaa atacagcacc
     2701 ggcttccgcg caccgacttc ggacgaaacc tggctgctgt tcccacaccc cgatttctac
     2761 ctgaaaacca atcccgaact gaaagcggaa aaagccaaaa actgggaatt gggcctggcg
     2821 ggcagcggca aagctggcag cttcaagctt tcgggcttca aaaccaaata ccgcgacttt
     2881 atcgaattga cgtatatggg cgtttcgtca gacgataaaa acaaccccag atacgccccg
     2941 ctttcagacg gcacggcatt ggtcagctcg cccgtttggc aaaaccaaaa ccgcaccgcc
     3001 gcctgggtgg aaggcataga gtttaacggc acttggaacc tcgacagcat cgggctgccc
     3061 aaagggctgc acaccggcct caacgtcagc tacatcaagg gcaaggcaac gcaaaacaac
     3121 ggcaaagaaa cgcccatcaa cgcgctttcg ccgtggacgg cggtttacag cctgggctat
     3181 gacgcgcctt ccaaacgctg gggcgtcaac gcctacgccg cgcgcaccgc cgccaaaaag
     3241 ccgtccgaca ccgtccacag caacgacgac ttgaacaaac cgtggcctta tgccaaacac
     3301 agcaaggcct atacgctgtt cgacctttcc gcctacctca acatcggcaa acaggttacg
     3361 ctccgcgccg ccgcgtacaa cattaccaac aagcagtact acacttggga atctttacgc
     3421 agcgtccgcg agttcggcac ggtcaaccgc gtcaacaaca aaacccacgc cggcatccaa
     3481 cgctttacct cgccgggcag gagctacaat ttcaccatcg aagcgaagtt ctaagctcct
     3541 gatgtgccaa tgccgtctga aacccgatga tttgggtttc agacggcatt ttttacggtc
     3601 gcaccgccgc cccctttcag gcttgtacca agcccaatcc cgcaactctc cgtcattccc
     3661 gcaactctcc gtcattccca gggaagtggg aatctagaaa cgcaaagcca caggaactta
     3721 tccgaaacaa cagcaaccta aaattcgtca ttcccacgaa agtgggaatc cagttcgttg
     3781 agtttcagtt atttaaaata aattttgaaa ctttaatccc atcattcccg cgaaagcggg
     3841 aatccggaac gtaaaatcta aagaaaccgt tttatccgat aagtttccgt ac
//
LOCUS       DAWTHD010000133         3806 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWTHD010000133 DAWTHD010000000
VERSION     DAWTHD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.133"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            124..903
                     /gene="map"
                     /locus_tag="WM704_001965"
     CDS             124..903
                     /gene="map"
                     /locus_tag="WM704_001965"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFA9341310.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            1002..1301
                     /locus_tag="WM704_001966"
     CDS             1002..1301
                     /locus_tag="WM704_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9341311.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            1539..1910
                     /locus_tag="WM704_001967"
     CDS             1539..1910
                     /locus_tag="WM704_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9341312.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(2097..3563)
                     /locus_tag="WM704_001968"
     CDS             complement(2097..3563)
                     /locus_tag="WM704_001968"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFA9341313.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT          975 a         1095 c          944 g          792 t
ORIGIN      
        1 cccaaagaaa accataccaa accggacacc ccgaaaaaca cgccggccaa accccataaa
       61 gagattctcg acaacctctt ttgacccggc acggcaggca cacccgcaat ccaaggaagc
      121 attatgaacg gcatcatcat caaaaccccc gaagaaatcg aaaaaatgcg cgagctgggc
      181 aaactcgtcg ccgaagccct cgactacatc ggacaattcg tcaaacccgg catcaccacc
      241 gacgaaatcg acaaacttgt ttacgactac cacgtcaacg tccaaggcgg ctatcccgcc
      301 cccctccact acggcaaccc gccctacccc aaatcctgct gcacctccgt caaccacgtc
      361 atctgccacg gcattcccga cgacaagccg ctcaaagaag gtgacattat caacatcgac
      421 ctcaccatca aaaaagacgg tttccacggc gactccagcc gtatgtttac cgtcggcaaa
      481 gtctccccca tcgcccaacg cctgatcgac gtaacccacg cctctatgat ggcgggcata
      541 gaagccgtca aacctggcgc gacgctgggc ggcgtaggtt acgcctgcca gcaggttgcc
      601 gaaaatgcag gttattcggt tgtacaagaa ttctgcggac acggcatcgg gcgcggtttc
      661 cacgaagccc cgcaagtgtt gcactacgga aaaaaagggc agggtcccgt tctaaaaccg
      721 ggtatgattt ttaccgtcga accgatgatc aaccaaggca aacgccacct gcgtatcctc
      781 aacgacggct ggacggtggt taccaaagac cgctccctct ccgcccaatg ggaacacgaa
      841 gtcttggtga ccgaaaccgg ctacgaaatc ctcaccgtca gccccgccac cggcaaaccc
      901 tgaagctgta tgcaatataa aaacaggctt gacccggcac attacgaaaa caaagcaaat
      961 cggaatttgc cccgcaacca gaaaaactta aaggaagttt tatgaaaata tttgaaaata
     1021 tagaagatgt taaagccatc cgtaaaaaga ccgggatgaa ccagatagac ttctggggca
     1081 aggtcggcgt tactcaatcc ggaggttcac gctacgaaac cggccgcaag atgcccaaac
     1141 ccgtacgcga actgctccgc ctcgtccata tcgaatgcct cgatttggca aaagtcaaca
     1201 aaaaagatat ggaaatcgcc gccctgttga aaaaacacca tcccgacctg tatgccgaat
     1261 tgtccaaaca gaccaaggcc gaaagaaaaa aacaaggtta aaccgcaacc tccggatgcc
     1321 cggaggtttt tcatttccga aaaatgcaaa caatgccgtc tgaaacaccg gacaggccgc
     1381 cgtatcccgc ctgccgcccc tgcctcaaac cgccgaaccg cccgaacctg cctttttaca
     1441 aactttatcc aatttcctgt ttatttcagg atacgccgac attagactgt caaacagtcc
     1501 gaaacgggca aactccacat ctatccaaag gaataaaaat gaaacttctg accactgcaa
     1561 tcctgtcttc cgcaatcgcg ctcagcagta tggccgccgc cggcacggac aaccccaccg
     1621 ttgccaaaaa aaccgtcagc tacgtctgcc agcaaggtaa aaaagtcaaa gtaacctacg
     1681 gcttcaacaa acagggtctg accacatacg cctccgccgt catcaacggc aaacgtgtgc
     1741 aaatgcccat caatttggat aaatccgaca atatggacac gttctacggc aaagaaggcg
     1801 gttatgtgct gagcaccggc gcaatggaca gcaaatccta ccgcaaacag cctattatga
     1861 ttaccgcacc tgacaaccaa atcgtcttca aagactgttc cccacgttaa tcaggcaaca
     1921 aaaaacagcg ttttcatttc tgaaaacgct gttttttgac cgttccatta ttcgcaaaag
     1981 ggaaaaaacg attacctgcc ccgtgtatca aaacctgccc tgccggatga agggcataac
     2041 cggcagggac ggcgtcaaca ccatatgggg gtacggcttt tcttgaaaga ttcggtttaa
     2101 atatccaaca ctttcgcggt ataggcgata atttcatccg ccctttcagg gttttcgttc
     2161 aacttgatgc cgtaacccgg taccagctct ttcagacgac cttcccaaga cggggcgcgc
     2221 tcggggaagc attggtgcat cagccggatc atcagcggca cagcggtcga tgcgcccggc
     2281 gacgctccca gcaatgcggc gagcgagccg tcggcgtggg cgacaatctc cgtaccaaac
     2341 tgcagcacgc cgcctttttc ggagtctttt ttaatgattt ggacgcgttg ccctgcggtg
     2401 atgagttccc agtcgtcggg gtttgcctcg gggtagtatt ccagcaggga ggcaaagcgt
     2461 tcttctttgg ttttacgcaa ttcgcccagc aggtatttgg tcagcggcat attcgcccag
     2521 ccggcgcgca gcataggata gaggttgtcc atatggatgg acagcggcaa atccataaac
     2581 gagccttgct tgaggaagtt ggaacggaaa cctgcgtaag gaccgaacat aaggtgtcgt
     2641 ttgccgtcta cgttgcgtgt gtcgaggtgc gggacggaca tcggcggcgc gccgacggaa
     2701 gcctgcccgt acactttggc gttgtgttgt tcggcggttt cggggttgct gttgcggaag
     2761 aacaggccgg acacgggtaa gccgccgtag cctttgcctt cggggatgcc ggatttttgc
     2821 agcagggtca gtgcgccgcc gcccgcgccg aggaagagga agcgggtgcg gagggtgagc
     2881 tgccagtctg ggttgcgggt atcggcggtt ttgagcaccc acgcgccgtc ggattcgcgt
     2941 ttgatgtctt cgacgtggcg gttgaactcg gtttttacgc ccttgccctg caaatatttc
     3001 accatctggc gcgtcagccg tccgaaatcg acatccgtgc cttcggcgga atagttggcg
     3061 gcgacgggtt ggttttcgtc ccggccgcgc ataatcagcg gagcccaatc ggaaattttg
     3121 ttccgatcgg tggaaaattc catattttca aaaagtttct gcgttttaaa cacatcatag
     3181 cgtttttgca ggtaacggca gtggtcttcg ttcatcacca aagacatatg cggcacggca
     3241 ttgatgaagg aattgtcttc caacttgcct tccgcgacca gcgtcgccca aaactggcgg
     3301 ctgacatgaa actgttcggc aatattgagg gcgcgcgccg gattgatgac gccgtccgca
     3361 cccagcggcg catagttcaa ttcgcacagc gcggaatgcc ccgtgccggc gttgttccac
     3421 gcgtttgacg attccaacgc cacatcttcc aagcgttcaa tcagggtgat ttcccaagac
     3481 ggttcgagtt ctttgagcaa aacgcccaaa gtcgcgctca taatgccgcc gcccaccaag
     3541 acaacgtctg tcgcttcagc catggtttac tcctaaaaaa caggcatctt ctgcccttat
     3601 ggttatttgc cgtactacaa acgcctgaat cgcaaaagca ggggaaaccg gcaatggtgt
     3661 gtgtccgagc acgctgtttc ggggttggaa tgcgttgcaa gtatggcttc cgacaccgct
     3721 tcaggggctt gtaatatgtt atcgtgaatg tagtggattt tactgggaaa tgcaaagttt
     3781 ttctgtcgcc cgccaagccg gggaac
//
LOCUS       DAWTHD010000134         3789 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWTHD010000134 DAWTHD010000000
VERSION     DAWTHD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.134"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            1..261
                     /locus_tag="WM704_001969"
                     /pseudo
     CDS             1..261
                     /locus_tag="WM704_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            694..906
                     /locus_tag="WM704_001970"
     CDS             694..906
                     /locus_tag="WM704_001970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HFA9341314.1"
                     /translation="MTNIKNMINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYG
                     RKEHGYNENYKNKLQKLYGVYAEGSA"
     gene            887..3570
                     /locus_tag="WM704_001971"
                     /pseudo
     CDS             887..3570
                     /locus_tag="WM704_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261022.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         1032 a         1013 c         1011 g          733 t
ORIGIN      
        1 tcgtcgctat gatgtattgg atgcgggcga atttagccat ttccgtatca atcacagcac
       61 acattttgcc gaacgacaaa accatattaa tggaattggg aacttttgga accgggcaaa
      121 acgtcattta cgcaagtttg acggcattcc caaagagcat tttgagccgt atttaaagga
      181 gtgcgaacgg cgttttaaca acagtgagat aaaagttctt gttccatttt aaaacaatta
      241 gtaaaatcga gtttatccta gttgtccagg acagatataa ttcaaaatat aaaaatgggt
      301 tacatctgga cattacgaaa tttttattcc ttcgcctgaa ttctattgtc agattcaaag
      361 agacctcatc atgcgaacca cctcaacctt ccctacaaaa actttcaaac cggctgccat
      421 ggcgttagct gttgcaacaa cactttctgc ctgcttaggc ggcggcggag gcggcacttc
      481 tgctcccgac tttaatgcag gcggcaccgg tatcggcagc aacagcaggg caacgatagc
      541 ggaatcagca gcagtatctt acgccggtat aaaaaacgaa atgtgcaaag acagaagcat
      601 gctctgtgcc ggtcgggatg acgttgcggt tacagacagg gatgccaaaa tcaaagcccc
      661 cgaatctgca taccagagac ttttcaaacc caaatgacca atattaagaa tatgatcaac
      721 ctcaaacctg caattgaagc aggctataca ggacgcgggg tagaggtagg tatcgtcgat
      781 acaggcgaat ccgtcggcag catatccttt cccgaactgt atggcagaaa agaacacggc
      841 tataacgaaa attacaaaaa caaattacaa aaactatacg gcgtatatgc ggaaggaagc
      901 gcctgaagac agaggcggta aagacattga agcttctttg gacaatgagg ccgttataga
      961 gactgaagca aagccgacgg atatccgcca tgtaaaagaa atcggacaca tcgatgtggt
     1021 ctcccatatt attggcgggc gttccgtgga cggcagacct gcaggcggta ttgcgcccga
     1081 tgcgacgcta cacataatga atacgcatga tggaaccaag aacgagaaat aatgtctgca
     1141 gccatccgca atgcatgggt caagctgggc gaacgtggcg tgcgcatcgt caataacagt
     1201 tttggaacaa catcgagggc aggcactgcc gaccatttcc aaatagccaa ttcggaggag
     1261 caataccgcc aaacgttgct cgactattcc ggcggtgata aaacagatga gggtatccgc
     1321 ctgatgcaac agagcgatta gggcaacctg tcctaccaca tccgtaataa aaacatcctt
     1381 ttcatctttt cgacaggcaa tgacacacaa gctcagccca acacactgac cctattgcca
     1441 ttttatgaaa agacgctcaa aaaggcatta tcacagtcgc aggcgtagac cgcagtggag
     1501 aaaagttcaa tggctccaac cattgcggaa ttactgccat gtggcgcctg tcggcatcct
     1561 atgaagcaac cgtccatttc acccgtacaa gaccgattca agttgccgga acatcctttt
     1621 ccgcacccat cgtaaccggc acggcggctc tgctgcagag atacccgtgg atgagcaacg
     1681 acaacctgcg taccacgctg ctgacgacgg ctcaggacat cggtgcagtc ggagtggaca
     1741 gcaagttcgg ctgggggctg ctggatgcgg gtaaggccat gaacggaccc gcgtcctttc
     1801 cgttcggcga ctttaccgcc gatacgaaag gtacatccga tattgcctac tccttccgta
     1861 acgacatttc aggcacgggc ggtctgatca aaaaaggcgg cagccaactg caactgcacg
     1921 gcaacaacac ctatacgggc aaaaccgtta tcgaaggcgg ttcgctggtg ttatcggata
     1981 tgcacgtcaa aaccaaaggt gcactgattt ataacggggc ggcatccggc ggcagcctga
     2041 acagccacgg cattgtctat ctggcagata ccgaccaatc cggcgcaaac gaaaccgtac
     2101 acatcaaaag gcggtctgca gctgggcgac aaaggtacgc tgtacacacg tttgggcaaa
     2161 ctgctgaaag tggacggtac ggcgattacc gacggcaagc tgtacatgtc ggcacgcggc
     2221 aagggggcag gctatctcaa cagtaccgga cgacatgttc ccttcctgag tgccgccaaa
     2281 atcgggcagg attattcttt cttcaaaaat atcgaaaccg acggcggtct gctggcttcc
     2341 ctcgacagcg tcgaaaaaac agcgggcagt gaaggcgaca cgccgtccta ttatgtccgt
     2401 cgcggcaatg cggcacggac tgcttcggca gcggcacatt ccgcgcccgc cggtctgaaa
     2461 cacgccgtag aacagggcgg cagcaatctg gaaaacctga tggtcgagct ggatgcctcc
     2521 gaatcatccg caacacccga gacggttgaa actgcggtcg ccgaccgcac agatatgccg
     2581 ggcatccgcc tacggcgcac aactttccgc gcagcggcag ccgtacagca tgcgaatacc
     2641 gccgacggcg tacgcatctt caacagtctc gccgctaccg tctatgccga cagtgccgcc
     2701 gcccatgccg atatgcaggg acgccgcctg aaagccgtat cggacgggtt ggaccacaac
     2761 ggtacgggtc tgcgcgtcat cgcgcaaacc caacaggacg gtggaacgtg ggaacagggc
     2821 ggtgtcgaag gcaaaatgcg cggcagtacc caaactatcg gcattgccgc gaaaaccggc
     2881 gaaaatacga cagcagccgc cacactgggc ataggacgca gcacatggag cgaaaacagt
     2941 gcaaatgcaa aaaccgacag cattagtctg tttgcaggca tacggcacga tgtgggcgat
     3001 atcggctatc tcaaaggcct gttctcctac ggacgctaca aaaacagcat cagccgcagc
     3061 accggtgcgg atgaatatgc ggaaggcagc gtcaacggca cgctgatgca gctgggcgca
     3121 ctgggtggtg tcaacgttcc gtttgccgca acgggagatt tgacggttga aggcggtctg
     3181 cgccacgacc tgctcaaaca ggatgcattc gccgaaaaag gcagtgcttt gggctggagc
     3241 ggcaacagcc tcactgaagg cacactggtc ggactcgcgg gtctgaagct gtcgcaaccc
     3301 ttgagcgata aagccgtcct gtctgcgacg gcgggcgtgg aacgcgacct gaacggacgc
     3361 gactacgcgg taacgggcgg ctttaccggc gcggctgcag caaccggcaa gacgggtgca
     3421 cgcaatatgc cgcacacccg ccgggttgcc ggtctggggg tggatgtcga attcggcaac
     3481 ggctggaacg gcttggcacg ttacagctac accggttcca aacagtacgg caaccacagc
     3541 ggacaaatcg gcgtaggcta ccggttctga cggacagaaa acagacagcc gcaaagatca
     3601 cggtctttgc ggctgtttct tatgaaaaga aaaccctatt ccaattgcct gcttctattg
     3661 tttcaagact tcttccaaag attcggcatc aatcagacgt atagcggatt aacaaaaatc
     3721 aggacaaggc ggcgggccgc aggcagtaca aatagtacgg aaccggttcg cccggtgctt
     3781 cagcacctt
//
LOCUS       DAWTHD010000135         3649 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWTHD010000135 DAWTHD010000000
VERSION     DAWTHD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.135"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..1617
                     /locus_tag="WM704_001972"
     CDS             <1..1617
                     /locus_tag="WM704_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9341315.1"
                     /translation="NTENSNNDNCKISSFGNYGPILIKSDIFALASQIKNSHINSEIL
                     SVGNYIEWLRPTLNKLTGWQEHLYAGLDPFHYIEVTDNSHVIGQTIDLGALELTNSLW
                     EPRWNSNIDYLITKNAEIRFNTKNESLLVKEDYAGGARFRFAYDLKDKVPEIPVLTFE
                     KNITGTSDIIFEGKALDNLKHLDGHQIVKVNDTADKDAFRLSSKYRKGIYTLSLQQRP
                     EGFFTKVQERDDIAIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLRVIDGHSN
                     QWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFRNPDTDNLTT
                     GNVKGFGAGVYATWHQLQDKQTGAYVDSWMQYQRFRHRINTEYATERFTSKGITASIE
                     AGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAQVNLLGSRQLQSRVG
                     VQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEIDGDRRVINNKTVIETQLGVAAKIK
                     SHLTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(1600..1896)
                     /locus_tag="WM704_001973"
     CDS             complement(1600..1896)
                     /locus_tag="WM704_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341316.1"
                     /translation="MVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKAVVI
                     NAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(1946..3313)
                     /locus_tag="WM704_001974"
     CDS             complement(1946..3313)
                     /locus_tag="WM704_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFA9341317.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(3423..>3649)
                     /locus_tag="WM704_001975"
     CDS             complement(3423..>3649)
                     /locus_tag="WM704_001975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341318.1"
                     /translation="LAACGGEKAAEAPAAEASSTEAPAAEAPAAEAPAAEAPAAEAPA
                     AEAPAAEAPAAEAAATEAPAAEAPAAEAAK"
BASE COUNT          999 a          781 c          922 g          947 t
ORIGIN      
        1 aatactgaga attccaataa tgataattgt aaaatttcat ccttcggaaa ctatggccca
       61 atattaataa agagcgatat tttcgcctta gcatcacaaa taaaaaatag ccatatcaac
      121 agcgaaattt tatctgtagg taattatatc gaatggcttc gcccaacact taataagctt
      181 actggttggc aagagcatct atatgctggt ttagatccat tccactatat tgaggttaca
      241 gacaactcgc acgttatcgg acaaacgatt gatttgggag cattagaatt aacaaattct
      301 ctatgggaac cccgttggaa ctctaatatt gattatttaa taacaaaaaa tgctgaaatt
      361 cgtttcaaca ctaaaaatga aagtttactc gtaaaagaag attatgctgg cggagctcgt
      421 tttcgttttg cttacgatct aaaagataaa gtccctgaaa taccagtttt aacttttgaa
      481 aaaaatataa ctggcacatc agatattatt tttgagggaa aagcgttgga taatttgaaa
      541 cacctagacg ggcatcaaat tgtcaaagta aatgacaccg cagataaaga tgcctttaga
      601 ctttccagta aataccgaaa gggaatttat acgctctctt tacaacaacg cccagagggc
      661 ttttttacca aagtgcaaga acgcgatgat attgcgattt atgcacaaca ggctcaagcc
      721 gccaatacct tattcgcctt gcgtttgaac gacaaaaaca gtgatatttt cgaccgcact
      781 ttaccacgca aaggcttgtg gttgcgtgtg attgacggac attccaacca atgggtgcaa
      841 ggcaaaacag cacctgtaga aggctatcga aaaggtgtgc aactcggcgg cgaggtgttc
      901 acatggcaaa acgaaagtaa tcaactttct attggcttaa tgggcggaca agcagaacaa
      961 cgcagcactt tccgcaatcc ggatacagac aatcttacaa cgggaaatgt gaaaggcttt
     1021 ggtgcgggcg tttacgccac ttggcatcag cttcaggaca aacagacagg tgcgtatgtt
     1081 gatagctgga tgcaatatca acgtttccgc caccgcatta acactgaata tgctacagaa
     1141 cgttttactt caaaaggtat tactgcctca attgaagcgg gttacaatgc gttattggcg
     1201 gaacacttca ctaaaaaggg caacagcctt cgtgtttacc tacaaccaca ggcgcaattg
     1261 acttatttgg gggtaaacgg aaaattcagc gatagcgaaa atgcccaagt gaatttgctt
     1321 ggctcccgcc aattacaaag ccgagtgggc gttcaagcta aagcgcaatt tgctttcact
     1381 aatggcgtca ctttccaacc atttgttgct gtcaattcaa tctaccaaca aaaacctttc
     1441 ggtgtggaaa tagacgggga tcgtcgagtg ataaacaata agacggtaat tgaaactcaa
     1501 ttaggtgtag cagcgaaaat caaatctcac ttaaccttgc aagcctcatt caaccgccaa
     1561 acaagcaaac atcatcacgc taaacaaggt gcgttgaatt tacagtggac gttttaagtt
     1621 aaattgtaac aaaataaaat cggcagggaa attcactgcc gattttatta ttttaaaagt
     1681 gcggtcattt ttcgtgtaat ttccaccaat ttttaccgca ctttcaattt cagcttgaac
     1741 aagttcaata gtctgctctg cattaattac gactgctttt ggattatctt ttactaattc
     1801 taaatagcgt gctcgagtgc gatggaaaaa atctaaatcc atttgctcaa tacggtctaa
     1861 ctcgccacgt ccacgagctc gcgctaaacc gacaatcgga tctattgtcc ttttctttgt
     1921 tgtgtatgca aatcagaaat gccggttacg gaagcagttt gccaggattc ataatgttat
     1981 atggatcaag gtgttgtttg atgcttttca tcagggcaat ttcagagggc gtgcggacag
     2041 cgggcagcca ttgttttttg atgataccta tgccgtgttc ggcggcaatc gtgccgttgc
     2101 gggcaaggac gttgcgatag actgtgctgt tgatgtcgtt ttcgtaacgg taaacttcat
     2161 tgctgaggat ttcgggcgga aaagtattgt agtgcaggct gccgtcgccc agatgtccga
     2221 agcagacgat ttgtatgcct ttaaaattct gttccaaatc tttggcgcac cggcggacaa
     2281 agtcggcaac gcgcccgata ggaacggcaa tatcgtgttt gatgctggtg cccagtttgc
     2341 gttgggaggc ggagatgttt tcgcgcaacg cccacatatg gatacgttct tgttcgcttt
     2401 gcgccaatac gctgtcggta aatccttttt tataaagaaa ttcgacaagc cgatcatcaa
     2461 gattgctgtc gggtaatgag tcggtcaact caagtaaaat atgccattct gaatgtgtcg
     2521 gcagggggag tttgctgaat tcggaagaca actcggcggc aaaacggccg atcagttcaa
     2581 aactgcataa gcgttcggca aagtgcgctt gggtttcggt cagcaggcgg acggcggatt
     2641 cgatgtcggg aatgccgacc catgccgttg ccttgtccga gggacgggca aaaagcttga
     2701 gcgtggcggc agtgatgatg cccagtgtgc cttcgctgcc gataaacaga tggcgcaggt
     2761 cgtagccggt ggtgttttta tgcaggggat ggagatggga aaccagttcg ccgttgggga
     2821 ggacgacttc caaaccgata accaggtcgc gcatcgtgcc gtaacgcaat acgttcaaac
     2881 ctccggcatt gcaggcgatg ttgccgccga tttggcacga gccttcgctg gcgagactga
     2941 gtgggaacag cctgtttgag gcttcggctg cctgttggac ggtttggagt acggaacctg
     3001 cttcgacggt tatgcagttg tctgacaaat tgatgctgcg gatgcggttg agtttggaaa
     3061 ggttcagcaa tacgccgttt tccgatactg ccgcgccgca caaaccggta ttgccgcctt
     3121 gcggcgtaac cggaatgcgg tgttcgtaac aaaaacgcat aatggtttgc acgctttcaa
     3181 cgctgcgcgg ctgcaaaatg atgtctggtg cagacgtaaa gcggttgcgc tgatctttta
     3241 aaagcgtcgg agaaggttcg gcaatttcat ctgccggcag gagtcgggaa aattcggtat
     3301 gaaggtcagg catgtttgtc ggaagggtag ggagaagatt gaggaaggga atctgatgcc
     3361 gtctgaaaaa tcaaaaaagc aggatactga acgtatcctg cttttttgca agcggaaaat
     3421 gcttattttg cagcttcggc agcaggagct tcagcggcag gtgcttcggt agcagcagct
     3481 tcggcggcag gagcttcagc agcgggagct tcggcggcgg gagcttcggc ggcaggtgct
     3541 tcggcggcag gtgcttcggc ggcaggtgct tcggcagcag gtgcctcggt agaggaagcc
     3601 tcagcagcag gagcttcagc ggctttttct ccgccgcagg cagccaaag
//
LOCUS       DAWTHD010000136         3605 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWTHD010000136 DAWTHD010000000
VERSION     DAWTHD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.136"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            100..975
                     /locus_tag="WM704_001976"
     CDS             100..975
                     /locus_tag="WM704_001976"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFA9341319.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            984..2180
                     /gene="bamC"
                     /locus_tag="WM704_001977"
     CDS             984..2180
                     /gene="bamC"
                     /locus_tag="WM704_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFA9341320.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2237..3169)
                     /gene="pip"
                     /locus_tag="WM704_001978"
     CDS             complement(2237..3169)
                     /gene="pip"
                     /locus_tag="WM704_001978"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFA9341321.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     VSPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT          975 a         1166 c          801 g          663 t
ORIGIN      
        1 ttggaaccaa ccgtgcggcc gatatgtttc acacaaacaa caaagtaagc tacaatccga
       61 agcataatcg acctcactca atgaataaaa ggtttccgta tgttacaagg tagcttggtt
      121 gccctgatta ccccgatgaa tcaagacggc agcatccatt acgaccaact ccgccaatta
      181 atcgactggc acatcgaaaa cggcacggac ggcatcgtcg ctgtcggcac gacaggcgaa
      241 tccgccaccc tctccgtcga agaacattta agcgtaatcg aagaaacggt caaacacgtt
      301 gccaaacgcg tccccgtcat cgccggcaca ggggcgaaca acaccgtcga agccatcgcc
      361 ctttcccaag ccgccgaaaa agccggcgcg gactacaccc tctccgtcgt cccctattac
      421 aacaaacctt cccaagaagg catgtaccgc catttcaaag ccgttgccga agccgccgcg
      481 attccgatga tactctacaa cgtgcccggc agaaccgtcg tcagcatgaa caacgaaacc
      541 atcctgcgcc ttgccgaaat ccccaacatc gtcggcgtta aagaagcaag cggcaacgtc
      601 ggcagcaaca tcgaactcat caaccgcgcg cccgaaggct tcgtcgtcct ttccggcgac
      661 gaccacaccg ccctgccgtt catgctctgc ggcggacacg gcgttattac cgtagccgcc
      721 aacgccgccc cgaaactctt tgccgatatg tgccgtgccg cgctgcaagg cgacattgcc
      781 cttgcacgcg aactcaacga ccggctgatt ccgatttacg acaccatgtt ctgcgaaccc
      841 agtcccgccg caccgaaatg ggcggtatcc gccttgggca gatgcgaacc gcacgtccgc
      901 ctgccgctcg ttccgctgac ggaaggcgga caggcaaaag tccgcgccgc cctgaaagcc
      961 tcaggacaac tttaaatata gccatgccgt ccgaaccgtt cggacggcat aacatgacaa
     1021 acactttaat atccatcaca caggatgaca cgatgaccca tatcaaaccc gtcattgccg
     1081 cgctcgcact catcgggctt gccgcctgct ccggcagcaa aaccgaacag cccaagctcg
     1141 actaccaaag ccggtcgcac cgcctgatca aactcgaagt cccgcctgat ttgaacaacc
     1201 ccgaccaagg caacctctac cgcctgcctg ccggttcggg agccgtccgc gccagcgatt
     1261 tggaaaaacg ccgcacaccc gccgtccaac agccagccga tgccgaagta ttgaaaagcg
     1321 tcaaaggcgt ccgcctcgag cgcgacggca gccaacgctg gcttgtcgtt gacggcaaat
     1381 cccccgccga aatctggccg cttctgaaag ccttttggca ggaaaacggc ttcgacatcg
     1441 aatccgaaga acccgccatc ggacaaatgg aaaccgagtg ggcggaaaac cgtgccaaaa
     1501 tcccccaaga cagcttgcgc cgcctattcg acacagtcgg tttgggcggc atctactcca
     1561 ccggcgagcg cgacaaattc atcgtccgta tcgaacaggg caaaaacggc gtttccgaca
     1621 tcttcttcgc ccacaaagcg atgaaagaag tgtatggcga caaaaacaaa gacacgacca
     1681 tgtggcagcc ttccgcttcc gaccccaacc ttgaggccgc tttcctgacg cgctttatgc
     1741 aatatttggg cgttgacgga cggcaggcgg aaaacgcatt ggcaaaaaaa ccgacccttc
     1801 ccgccgccaa cgaaatggcg cgtatcgaag gcaaaagcct gattgtcttt ggcgactacg
     1861 gcagaaactg gcggcgcacc ggccttgccc tcgaccgcat cggactgacc gtcgtcggtc
     1921 aaaacaccga acgccacgcc ttcctggttc aaaaagcccc gaacgaaagc aatgcagtta
     1981 ccgaacaaaa accggggctg ttcaaacgcc tactgggcaa aggcaaagcg gagaaacctg
     2041 ccgaacagcc ggaactgatt gtctatgccg agcctgtcgc cgacggttcg cgcatcgtcc
     2101 tgctcaacaa agacggcagc gcatatgccg gcaaagacgc atccgcactg ttaggcaaac
     2161 tccattccga actgcgttaa accgttcaaa acaaaagcag cttccaaaca gaagctgctt
     2221 ttttgtgcgg aactttttac aacaaacggg gcaaaatatc ctcaaccgcc tgaaccaacg
     2281 catccgccaa aggcggatcg aacgcacaat gccccgcctg gaccaccctc aattccgctt
     2341 cgggaaaggc tttcgacagc tcccacgcac tctgcatagg cgtacacaaa tcataccgcc
     2401 cctgcacgat aacggtcggg atatgccgta ttttgccgat attgttcaaa atcgccttat
     2461 caccctgcaa ccaaccgccg ttgacaaaat aatggttttc caaacgcgcg attgccagtg
     2521 aggcataagc atcttcatcc actccctccg gctcgaaacg aatcagatag ctttcccaat
     2581 ccgcccaagc cttcgcggca gacaggcaca cttcttcatc ttgatgaaac agcaacccgt
     2641 gatacgcctc aatcagccgg ttccgccgat tttcagcaat cggcgcgaca aatttttgcc
     2701 attgttccgg ataaatccgg ctcacgccgc ccgcctcgtt cagccacgcc gtttcagacg
     2761 gcctgcacaa aaatatcccg cgcaacacca atccctttac ccgttcggga tgggtttggg
     2821 cataagccag cgacaaagtg ctgccccacg aaccgccgaa caccagccat ttcccgatac
     2881 ccagcatttc acggactttt tcaatatccg ccaccaaatc ccaagtcgta ttgtcttccg
     2941 cacaagcata cgggtgcgaa cgtccgcaac cgcgctggtc gatgatgacg atgcggaaca
     3001 catcaggatt gaaaaaaccc cgacattcag gcgaaacccc cgcgcccggc ccgccgtgta
     3061 aaaaaatgac cggcacaccg tcgggattgc ccgactcttc ccaataaatt tgatgaattt
     3121 cagacacctg caagtatccg ctatgaaaag gctgttttat ttcatacatc atcttctccc
     3181 atattgcttc tgacaatccg aacatcccga cacgcgacat cccgcacgaa acacaagccg
     3241 ccccgaaagg caaatgccga aaaatcgcga taaaaaccat caatcggaaa atattgcata
     3301 aacaggcgaa aatatatcat tgaaaagaaa acccatacaa tgtctgacag aaacaagccg
     3361 ctttgccatt gtttcctaac agttaaaccc cgcccttcaa ggcggcgggg cagggcttga
     3421 ttcaaaatgg cgcaagcccc tgccctcaaa tccaacacgc aggattaaac cataatagcg
     3481 gctttcttat tatttcttat tgaaacaccg cccggaaccc gatataatcc gcctttcaac
     3541 atcagtgaaa atcttttttt aaccggtcaa accgaataag gagccgaaaa tgaatccagc
     3601 ccgca
//
LOCUS       DAWTHD010000137         3543 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWTHD010000137 DAWTHD010000000
VERSION     DAWTHD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.137"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..692
                     /locus_tag="WM704_001979"
     CDS             <1..692
                     /locus_tag="WM704_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9341322.1"
                     /translation="VGEMEKDRSIIAMGAWLEVLSEENNKSVLAAIARNGAIWDKPTR
                     HEDIVAVFPFGNPIHNNTMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYP
                     EALVKYRFHQDQTSSKYNLQQRRTAWKIKEEIRAGYWKAAGIAVGADCLNYGLLKSTA
                     YALYEKALSGQDIGCLRLFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILK
                     KMLRPWKYRSY"
     gene            711..1553
                     /locus_tag="WM704_001980"
     CDS             711..1553
                     /locus_tag="WM704_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFA9341323.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLFGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISR
                     EAMRFFLDRFAVLPAERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKAMFDLKRALGKFGREKKKRKERQRQAELEKVYGRRV
                     ILFK"
     gene            complement(1896..2621)
                     /locus_tag="WM704_001981"
     CDS             complement(1896..2621)
                     /locus_tag="WM704_001981"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFA9341324.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSENTRLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            2725..3519
                     /locus_tag="WM704_001982"
     CDS             2725..3519
                     /locus_tag="WM704_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFA9341325.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          780 a          781 c         1089 g          893 t
ORIGIN      
        1 tcgtgggcga gatggaaaaa gaccgcagca tcattgcgat gggcgcgtgg ttggaagttt
       61 tgtcggaaga aaacaataaa agcgtgcttg ccgccattgc ccgaaacggc gcaatttggg
      121 acaaaccgac ccggcatgaa gacattgtcg ccgttttccc tttcggcaac cccatacaca
      181 acaacacgat gattatgagg cgcagcgtca ttgacggcgg tttgcggttc gatccagcct
      241 atatccacgc cgaagactat aagttttggt acgaagccgg caaactgggc aggctggctt
      301 attatcccga agccttggtc aaataccgct tccatcaaga ccagacttct tccaaataca
      361 acctgcaaca gcgcaggacg gcgtggaaaa tcaaagaaga aatcagggcg gggtattgga
      421 aggcggcagg catagccgtc ggggcggact gcctgaatta cgggcttttg aaatcaacgg
      481 catatgcgtt gtacgaaaaa gccttgtccg gacaggatat cggatgcctc cgcctgttcc
      541 tgtacgaata tttcttgtcg ttggaaaagt attctttgac cgatttgctg gatttcttga
      601 cagaccgcgt gatgaggaag ctgtttgccg caccgcaata taggaaaatc ctgaaaaaaa
      661 tgttacgccc ttggaaatac cgcagctatt gaaaccgaac aggataaatc atgcaaaacc
      721 acgttatcag cttggcttcc gccgcagagc gcagggcgca cattgccgat accttcggca
      781 gtcgcggcat cccgttccag tttttcgacg cactgatgcc gtctgaaagg ctggaacggg
      841 cgatggcgga actcgtcccc ggcttgtcgg cgcaccccta tttgagcgga gtggaaaaag
      901 cctgctttat gagccacgcc gtattgtgga aacaggcatt ggacgaaggc ttaccgtatg
      961 ttgccgtatt tgaggatgat gtcctgtttg gcaaagacgc agaaaagttc cttgccgaag
     1021 atacttggtt ggaagagcgt tttgataagg attccgcctt tatcgtccgt ttggaaacga
     1081 tgtttgcgaa agttattgtc agaccggata aagtcctgaa ttatgaaaac cggtcatttc
     1141 ctttgctgga gagcgaacat tgtgggacgg ctggctatat catttcgcgt gaggcgatgc
     1201 ggtttttctt ggacaggttt gccgttttgc cggcagagcg gattaaagcg gtagatttga
     1261 tgatgtttac ttatttcttt gataaggagg ggatgcctgt ttatcaggtt agtcccgcct
     1321 tatgtaccca agaattgcat tatgccaagt ttctcagtca aaacagtatg ttgggtagcg
     1381 atttggaaaa agatagggaa caaggaagaa gacaccgccg ttcgttgaag gcgatgtttg
     1441 acttgaagcg tgctttgggt aaattcggta gggaaaagaa gaaaagaaag gagcgtcaaa
     1501 ggcaggcgga gcttgagaaa gtttacggca ggcgggtcat attgttcaaa tagtttgtgt
     1561 aaaatatagg ggattaaaat cagaaatgga cacactgtca ttcccgcgca ggcgggaatc
     1621 cagtccgttc ggtttcgctt gttttaagtt tcgggtaact tccacttcgt cattcccgcg
     1681 aaagtgggaa tccggttcgt tgggtttcag tcatttccga taaatcaccg caacgttaaa
     1741 tttctagatt cccgcctgcg cgggaatgac ggcggagagg gtttgttgtt tcgggtaaat
     1801 ccctgtagtt tttcgtttcc agattcccgc tttcgcggga atgacggcgg ggggattgtt
     1861 gctttttcgg ataaaatccc gtgttttttc atctgctagg taaaatcgcc ccaaagcgtc
     1921 tgcatcgcgg cgatggcggc gagtggggcg gtttctgtgc gtaaaatccg ttttccgagt
     1981 gtaaccgcct gaaagccggc ttcaaatgcc tgttgttctt cctgttctgt ccagccgcct
     2041 tcgggcccga ccataaagac gattgcgccg gacgggtggc ggatgtcgcc gagtttgcgg
     2101 gcgcggttga tgctcataat cagcctggtg ttttcagacg gcattttgtc gagtgcttca
     2161 cggtagccga tgatgggcag tacgggggga acggtgttcc tgccgctttg ttcgcacgcg
     2221 gagatgacga tttcctgcca gcgtgcgagg cgtttggcgg cgcgttctcc gtcgaggcgg
     2281 acgatgcagc gttcgctgat gacgggctgt atggcggtta cgccgagttc gacgcttttt
     2341 tgcagggtga aatccatgcg atcgccggag gaaatggatt ggatgagcgt gatgttgagc
     2401 ggggactcgt tgtcggttgt gtcttcgtgc aggatttcgg cttcggcgcg gtgtttttcc
     2461 aaaacggtca gccgtgcggt atgtgccttg cctttgccgt cgaagagggt gatgttttcg
     2521 ttggggcgga cgcgcaggac gttgaggtgg cggacgatgt tgtcgggcag ggcgacggtt
     2581 tgtccgacgg aaaggttttc gggcaggtag aatctgggca tagtttttgg cggttcggac
     2641 ggttataatc cgtcgcagcg gcgtgatgga aacggacatt atacgttcgt gcgccgcaag
     2701 ttcaaaatat gaggacagta ggaagtgtta caccgtttgc agaaagtcgt gcgccatatc
     2761 gcgcaaaccg aaattatgcc gcgttttttg aatacgccgt cccgccgcaa ggaagacggt
     2821 tcgatgttga gcgaggcgga cattgccgcg cagacggcat ttgccgccgc gttgccgctt
     2881 ctgatcgatt gcccgatgtt gggcgaggaa atgtcgcggc aggaacagtc ggctttgtgg
     2941 gaacaatatt cgggggaaaa ggggctgtgg attgtcgatc cgatagacgg gacgaacaat
     3001 tttgtcaacg ggctgccgca ttttgccgtg tcggtggcgt ttgtccgcaa cgggcgcgcc
     3061 gaattgggcg taatctacaa tccggtcagc ggcgaatgtt tttatgccga acgcgggcag
     3121 ggtgcgtttc ttaatgggac acgcctgcct ttgcgtctcg tggataaaaa actcaatgag
     3181 gcgattgcgg gcgtggaaat caaatatctg cgttcgggca aactttccag ccgcatgagt
     3241 acgctcgcgc ctttcgggac gatacgcagt atgggcagca gcacgctgga ctggtgttat
     3301 ctggcgtgcg ggcgttatga tgtttatgtc cacggcgggc agaagctgtg ggattatgct
     3361 gcgggtgcgt tgattttcga ggaggcgggc ggcaggctga cgactttgga aggcgacggg
     3421 ttttggagtg gtgaacacgt gttcaaacgc tcggtcgtcg ccgcgctcga accgaagctg
     3481 tttgaacgct gggtcgggtg gatacgggaa aaccagtagg ttccagatat attcgttaat
     3541 ata
//
LOCUS       DAWTHD010000138         3474 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWTHD010000138 DAWTHD010000000
VERSION     DAWTHD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.138"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            368..>3474
                     /locus_tag="WM704_001983"
     CDS             368..>3474
                     /locus_tag="WM704_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9341326.1"
                     /translation="MNKTLKRRVFRHTALYTAILMFSHTGGGGAMAQTHQYAIIMNER
                     KQPEVKWKDQYNQSALKDKSRERTFSHTSQKNGLGITSNFISFNNNDELVSQQSGTAV
                     FGTATYLPPYGKVSGFDTAELNKRGNAVNWIHTTRPGLAGYGYTGIRCGSTKDCPKLT
                     YKTRFSFDNPDLLKTRGGLDRHTEPSRENSPIYKLKDYPWLGVSFNLGGEGTAKDGRS
                     SSKLVSSFDENNSNSNQNLVYTTEDHRISLSNWQQETTAMAYYLNAKLHLLDKKGIKD
                     ITGKTVRLGVLKPSIDVKTQNTGLSGILNFWSKWDIKDNGQIPVKLGLPEVKAGRCIN
                     EPNPNKNTQAPSPALTAPALWFGPGQNGKVQMYSASVSTYPGSSSSRIFLQELKTQTD
                     PARPGRHSLAALNARDIKSREPNFNSRQTVIRLPGGVYRIGPTRNGIVGLNGNDGKND
                     TFGIYKDRLVTPEVDEWAKVLLPWTVRYYGNDDIFKTFNQPNNKKQSDKKQYSQKYRI
                     RTKEDDNDKPRDLGDIVNSPIVAVGGYLATSANDGMVHLFKRNGTNQRGYELKLSYIP
                     GTMERKDIENQDSTLAKELRAFAEKGYVGDRYGVDGGFVLRRITDDQDREKHFFMFGA
                     MGLGGRGAYALDLTKIDSNNLTGVSMFDVQNDKNNNNNDSNRVKLGYTVGTPQIGKTQ
                     NGKYAAFLASGYAAKDIVSSDNTTALYVYDLKDTLGTPIAKIEAPGGKGGLSSPTLVD
                     KDLDGTVDIAYAGDRGGNMYRFDLSSDKPSEWAVRTIFEGTKPITSAPAVSRLADKRV
                     VIFGTGSDLSEEDVVGKDQQYIYGIFDDDKGTGTVKVTVQNGTGGGLLEQTLTKENNT
                     LFLSNNKASGGSADKGWVVKLREGERVTVKPTVVLRTAFVTIRSYTGMDKCGAQTAIL
                     GINTADGGALTPRSARPIVPDHNSVAQYSGHKKTTDGKSVPIGCMWKNSKTVCPNGYV
                     YDKPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDL
                     DSLDIT"
BASE COUNT          958 a          924 c          917 g          675 t
ORIGIN      
        1 attgccgaaa accgctttac catgatcagc gcgcaagggt agaataatgc ggtaacccta
       61 tacattgcat ccggccaaag gggcgtgtta cctttgcgaa catccccctt tggcagccgg
      121 gcgaaggggg ctttgcaacc ggcaatccgg cgggcgcggg atcgggcggt ttgcccaatc
      181 ccgccgtttg cggcgcgcct gccgccgacg gtatcccgcg aagcaagatt taagggataa
      241 aatatgttcc aacacgcagg gcggcacata aggcgccgcc ctgattcgga agggcttgca
      301 cccctcccgg acaaagcctg atcctgccgc cccgaagggc ggggggtttg accgaaaagg
      361 aaatacgatg aataaaactt taaaaaggcg ggttttccgc cataccgcgc tttataccgc
      421 catcttgatg ttttcccata ccggcggggg gggggcgatg gcgcaaaccc atcaatacgc
      481 tattatcatg aacgagcgaa agcagcccga ggtaaagtgg aaggatcaat ataatcaatc
      541 agcattaaag gacaaaagca gggagcggac atttagccat acgagccaga aaaacggcct
      601 cggcatcaca agcaatttta tctcattcaa caataacgat gagcttgttt ctcaacaaag
      661 cggtactgcc gtttttggca cagccaccta cctgccgccc tacggcaagg tttccggctt
      721 tgataccgcc gagctgaaca agcgcggcaa tgccgtcaat tggattcata ccacccggcc
      781 cgggctggca ggctacggct acaccggtat ccgttgcgga agtaccaaag actgtcccaa
      841 acttacctat aaaacccgat tttccttcga taatcccgac ttgctaaaaa caagaggcgg
      901 gctggatagg cacacagagc caagccgcga aaattcgccc atttacaaat tgaaggatta
      961 tccatggttg ggtgtgtctt tcaatttggg cggcgagggt accgccaaag atggcagatc
     1021 atccagcaaa ttggtatctt cttttgatga aaacaatagt aatagtaatc aaaacctcgt
     1081 ctatacgaca gaagaccacc gtatttccct tagcaactgg cagcaagaaa cgaccgccat
     1141 ggcctattat ctgaacgcca agctgcacct gctggacaaa aaagggatta aagatatcac
     1201 cggcaaaaca gtgcggttgg gtgtcttgaa gccgagcatc gatgtgaaga cacaaaatac
     1261 ggggcttagc ggcattctaa atttttggtc taagtgggac attaaagata acgggcagat
     1321 tccggtcaag ctcggcctgc cggaagtcaa agcaggccgc tgcatcaacg aaccgaaccc
     1381 caataagaat acccaagccc cttcgccggc actgaccgcc cccgcgctgt ggttcggccc
     1441 tgggcaaaat ggtaaggtgc agatgtattc cgcttcggtt tctacctacc ccggcagttc
     1501 gagcagccgc atcttcctcc aagagctgaa aactcaaacc gaccccgccc ggcccggccg
     1561 gcattccctc gccgctttga atgcgcggga tatcaaatcc cgcgagccga atttcaactc
     1621 aaggcagacc gtgatccgat tgccgggcgg cgtgtaccgg atcggcccga ctcgcaacgg
     1681 gatcgtgggt ttgaatggca atgacggcaa aaacgacact ttcggcatct acaaggacag
     1741 gctcgtcaca cctgaggtcg acgagtgggc aaaagtgctg ctgccttgga cggtccggta
     1801 ttacggtaat gacgatatat ttaaaacatt caaccaacca aacaacaaaa aacaaagcga
     1861 caaaaaacaa tacagccaaa aataccgcat ccgcacaaaa gaagatgaca atgacaaacc
     1921 ccgcgatttg ggcgacatcg tcaacagccc gattgtcgcg gtcggcgggt atctggcaac
     1981 ttctgccaac gacgggatgg tgcacctgtt taaaagaaac ggcacaaacc aacgaggcta
     2041 cgaactgaag ctcagctaca tccccggtac gatggagcgt aaggatattg aaaaccaaga
     2101 ctccaccctc gccaaagagc tgcgcgcctt tgccgaaaaa ggctatgtgg gcgaccgcta
     2161 cggcgtggac ggcggctttg tcttgcgccg cattacagat gaccaggaca gggaaaaaca
     2221 tttctttatg tttggtgcga tgggccttgg cggcagaggc gcatacgcct tggatttgac
     2281 caaaatcgac agcaacaacc tgaccggcgt ttccatgttt gatgtccaaa acgacaaaaa
     2341 taacaataac aatgacagta atcgcgtgaa attaggctac accgtcggta cgccgcaaat
     2401 cggcaaaacc caaaacggca aatacgccgc cttcctcgcc tccggttatg cggctaaaga
     2461 tattgtcagc agcgataata caaccgcgct gtatgtgtat gatttgaaag acaccttagg
     2521 tacgccgatt gcaaaaatcg aagcacccgg cggcaaaggc gggctttcgt cccccacgct
     2581 ggtggataaa gatttggacg gcacggtcga tatcgcctat gccggcgatc ggggcgggaa
     2641 tatgtaccgc tttgatttga gcagcgacaa gccgtccgag tgggctgtac gcactatttt
     2701 cgaaggcaca aaaccgatta cttccgcgcc cgccgtttcc cgactggcag acaaacgcgt
     2761 ggtcatcttc ggtacgggca gcgatttgag tgaagaggat gtagtcggta aggatcaaca
     2821 atatatttac ggtatctttg acgacgataa ggggacgggg acggttaagg taacggtaca
     2881 aaacggcacg ggaggcgggc tgctcgagca aacgcttacg aaggaaaata acaccttgtt
     2941 cctgagcaat aataaggcat ccggcggatc ggccgataaa ggctgggtag tgaaattgag
     3001 ggaaggagaa cgcgttaccg tcaaaccgac cgtggtattg cgtaccgcct ttgtcaccat
     3061 ccgcagctat acgggcatgg acaaatgtgg cgcgcaaacc gccattttgg gcatcaatac
     3121 cgccgacggc ggcgcattga ctccgagaag cgcgcgcccg attgtgccgg atcacaattc
     3181 ggttgcgcaa tattccggcc ataagaaaac caccgacggc aagtccgtcc ccataggctg
     3241 tatgtggaaa aacagcaaaa ccgtctgccc gaacggatat gtttacgaca aaccggttaa
     3301 tgtgcgctac ctggacgaaa agaaaacaga cgatttcccc gtcacggcag acggtgatgc
     3361 aggcggcagc ggaacattca aagagggtaa aaaacccgcc cgcaataacc ggtgcttctc
     3421 cggaaaaggt gtgcgcaccc tgctgatgaa cgatttggac agcttggata ttac
//
LOCUS       DAWTHD010000139         3422 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWTHD010000139 DAWTHD010000000
VERSION     DAWTHD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.139"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(241..456)
                     /locus_tag="WM704_001984"
     CDS             complement(241..456)
                     /locus_tag="WM704_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341327.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(487..3255)
                     /locus_tag="WM704_001985"
     CDS             complement(487..3255)
                     /locus_tag="WM704_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9341328.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEGHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          784 a          852 c          849 g          937 t
ORIGIN      
        1 acatatcatc tgcgcggtaa tgatgagatt ttaggttgtg ggatttatcg gaaaaacaga
       61 aacctctccg ccgccattct cacgaaagtg ggaatccgga aagctaaagc cacaggaatt
      121 tatcggaaat ggctgaaatt tatagtggat taacaaaaat caggacaagg cgacgaagct
      181 gcagacagta caaatagtac ggcaaggcga ggcaacgccg tacctgtttt tgttaatcca
      241 ctataaaaaa actgattccc gcttttgcgg gaatgacgac tcataaatta cccgaaacaa
      301 ccaaaaataa ccgaaatcaa aaaccggctt ccctgcgtgg gaatgaaatg gatttttctt
      361 tgagttgccg atgcgataaa acggattggc ataggcttgc ggcaggatga tgcgtttggg
      421 gaaacaaatg ccgtctgaag ggctttcaga cggcattgca gtttttccac aaaacgcggg
      481 ctgcctttaa aacttgtagc tcatcgtcat caagaaggtg cgtccgcgtg cgaaattggt
      541 caatacgctt ttgcttgtgc cgccgtattt gccgttgcac aacgttttat cagcattaca
      601 cgttacgtct tcgtccttgt ctttcgggtc gaacgagctg taataacgct gcgttgccgc
      661 atcattgccc gcatcgagcg gatcgatata acgcctgtcg aacaggtttt tgacttcggc
      721 gcggaaaata aggtttttct tcggctcgta agcggcgtaa aaatcaaaaa tcaaaggctg
      781 tcgggcaagg gtttcggttt gtttgatgga acgcttgccc agttgccgga cattgctggt
      841 atttcccccg ttggtgccgt cgatatagcg ttcttcagcc gtcgcgcgga tgctcttgcc
      901 gaaatagcgc atcgcgccgc ccaaagtcag tttgttgccc aaccagcgcg taccgacttc
      961 caaacgtccg taatctcgcg gcagggcgga aaccctgctc agcccataac cttgtttgag
     1021 ttggtcttct ttggaggcat tgttgggcga ttcgctcgca tcgctgaaat tggtcggttg
     1081 cgtgcttttt tgataggcgt aagaaaggtt ggtgaaaaaa cgcccataat cgtaattcag
     1141 ctccagctca aaaccgtgtt tgtgcacttt gtctttgaaa ttgcggtgtc ggatggtgta
     1201 ggcaagcccg gtgctgccga cccagctcgg aatatccccg ttcaaatccc accatttccc
     1261 gtaaacgttg tggatgtagt tgtcaatgcg gctgcggtag ccgaccagtt tcaatcctaa
     1321 tatatcatct tgttttaaca atcctttttt ataggtattg aagccaaatt gccaagtgtt
     1381 tgcgcgctct ggttttaagg cggtgtgaac gccggagtcg ccgatttggg aaaaatacat
     1441 ttcttggatg ttgggcatac ggtgtgtgcg cgaatagccg gcgaacggca tgaaataatc
     1501 gccgaagtcc gcactaatgc tgaccgaatg gttgttggcg cgctttttgc cgtatttttt
     1561 caatacgggt tcataaagcc cgcagctcgg gtcgcaatgt tccttgtatg ccggcgagtt
     1621 ttctccgaat gcccgcttaa attcgttttc cgagccgtaa tagcccgtat attcgccgcc
     1681 gaaacggtag ttgattgcat tggtgctgta gtttaagcgg taaatgtctt ttttgagcgc
     1741 ggcatcgaag tagaacgtgt tgaaatattg actgccggcc ggttggacga tggttgattt
     1801 ttggggcagc agccctttat cgcccttaaa ccgccccaaa taggaataaa gcccgttgtc
     1861 ctgatccgga ccgtcgaaaa acagccccaa ttcttcagga aagcggtttt tgccgtattc
     1921 gttgtggaaa taattgaagc ccaaagtggt ttgcaactcg gtttcgcggg gcagccggaa
     1981 ggtggcggtg ttgttgaggt cgaggatttt tgcgttgttg taggtttcaa aatcctttaa
     2041 aagcccccag cctgtaaact tcgacccttt cggatatttc tgcctgcccg aattgtaggc
     2101 tgcggtcaga ttgaggttgg tatacgggtt caaagacaaa ccgtaattga attgataatt
     2161 gcggttgatg attttgcggc tgccgatttt ggtgtttaaa tcgcgaaatt gcgccgtgta
     2221 tttattgaat acgccgtcgt attccaattt aaacagatta cctgccgact gctgcttcag
     2281 gccggacgga tcgatggggg tgatgtcgta ttgcggcgcc aggttttccc gccagctttt
     2341 gtcatgacct tcgatgtatt tttgcagttc ttgggggtct tcgtattttt tataccactt
     2401 tgttttccag tattgccttt gcaaatcccg ttcccatttt ccgctgccgg cattgaattt
     2461 caaaccaccc tcttgtacaa aatattgctg tttgcgccgt tccagatatt cttcaccaaa
     2521 atttccgatg tgctgcccgc cgccgcccac gcggtaattt tgcgccacgc cgcgcctgct
     2581 gtgcccgtaa agcacaccga cagatgctcc gctttccagc catttgcgcg cacctatcgc
     2641 cgccatcgca ttgcctttgg ttgaattggt gccggtcaga ccttttagca gcaggccgta
     2701 ggtattattg ccctgaacga cgtcatccac gcctaaagtc cgcagattcg ccgaaccggc
     2761 aaggctgttg atgcctgccg agccgctgaa gctgcctttg acgacatcca gtccggcaat
     2821 aaaattgctg tcgacagatg caccgaattg agatgaaccg cctgccctgc ccgcatcggt
     2881 agaagtcgaa taaaaggtct gcgtgatgcc gtccaccatc gtattgaccc gcccgaaccc
     2941 gctgtcgccg cgaatattca aagacacaat gcccgagctt ttatcttgct gtgtaaacgc
     3001 accgggtatg ctgcgtacga tgttgtcgag gttttcgccg gatttgaata tatcctgacg
     3061 ggtcgatacg gcacgcgcat cggtaaacac ttttttgtct ttcggtacgc gcttcgcctt
     3121 gacgtgcacg tcttccaaaa cctgtatctg cgcctcgctg cccgcgcgcc ctgcatcttc
     3181 tgcataacta tgatgatata gcataacacc cataagataa aaacaaatcg gcttcaaccg
     3241 gaaagaagat ctcatatttt cctcaacaat aaacaatcaa tccaacatgt ctaccgtttt
     3301 catcgaatcc gccttttcga ccacccggcc ctacgcaacc gaaccgtcat tcccacggaa
     3361 gtgggaatct aggacgcggg gtttcagtta tttccgatag attcccgccg cgtcaggggt
     3421 cc
//
LOCUS       DAWTHD010000140         3408 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWTHD010000140 DAWTHD010000000
VERSION     DAWTHD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.140"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            22..1287
                     /locus_tag="WM704_001986"
     CDS             22..1287
                     /locus_tag="WM704_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFA9341329.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            1290..3377
                     /locus_tag="WM704_001987"
     CDS             1290..3377
                     /locus_tag="WM704_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFA9341330.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHIIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
BASE COUNT         1162 a          605 c          594 g         1047 t
ORIGIN      
        1 ttttttaaga aataataata aatgtcccac ttattccgaa aagaagtctt tgtagcccaa
       61 caaaataagt ggacaggtca ggttatcttg acccgtccat tctctttttt atttctgact
      121 ttttgcgctt ttctcattgc tctgtgtatc attatctttt tgatttttgg tagctatacc
      181 aataaaacaa ccgttgaagg tcaattactt ccaactatgg gggtggttcg tgtttactct
      241 tccgatatcg gcacgattac gcataaattt gttgaagatg gtaactttgt caaagctggc
      301 gaaccattgt tcaaactttc cacatcgcgt tttggcgaaa aaggaaacgt acaagccaaa
      361 ttggcagcag aagccaacct taaaaaaact ttggcattac aagaattgga acgtttaaag
      421 cgcattcatc aaaatgagca aaaaaatgtt cataacaaca ttcatcgttt aaacaatcaa
      481 ttagagaata ttaaacagca aattacaggg caaaatcgtc aaattcgttt agcggaaaaa
      541 acccttaaca agaacaagtt tttagccagt caaggcgcag tatcccaaca agataagatg
      601 accgccgaaa gccatttatt ggaacaacgc tcacgtttgg agagcctaaa acgtgaacaa
      661 aataatgcaa tcagggaact tgatgaacag aaaatcacat taagcagcct gcctgaacgc
      721 cataaaaccg aattgagcca actcaaccgt gcgattacgg aaatgaacca agaaattttg
      781 gattttgatt tgaaatccga acaaaccata cgagctagta aatcaggtta tatttcaaca
      841 attaatgttg atatagggca acaagttgaa ccgtctaaat tgctgttaag cattgtccct
      901 gaacaaactg aattggtcgc caatctttac atacccagta aagctgttgg ttttattaaa
      961 ccgaaagata aagttgtttt acgttaccaa gcgtaccctt accaaaaatt tggacatgcc
     1021 acaggagaaa ttatttcagt tgccagaact gctctcggta aacaagagct atcaggttta
     1081 ggtatcattt tcactaaccc aaccttatta aatgaacctg cctatcttgt gaaagttaaa
     1141 ttggaaaaac aaacgattaa agcatacgga gaaaacaagc cgcttcaaat tggcatgatt
     1201 ttagaagcag atattctcca tgaacgaaaa aaattgtacg aatgggtact tgacccactt
     1261 tacagcattt caggaaaaat caattaaaaa tggattattt atcaagactg tcctttggat
     1321 ttaacaaaaa gctacctgtc attctgcaaa cagaagttgc tgaatgtggt ttagcatgcc
     1381 tgacatccat cttgtcctat tatggctttc acactgattt aagaacgtta cgccaaaaat
     1441 acaccctgtc attaaagggc gcaaatcttg cagacatcat gagatttggc aatgaaatga
     1501 atttaacgcc acgagctttg cgtttagagt tagatgagct gtcaaattta caactaccct
     1561 gcattctcca ttggaactta aaccattttg ttgtactttg ttccatttcc aaagacagta
     1621 tcgtcattat ggaccctgct gtcggtatgc gaaaaatcaa aatggacgaa gtttcacaaa
     1681 aattcacagg gattgcccta gaattattcc ccaataccca ttttgaagag aaaaaagaaa
     1741 caaagaaaat caaaatatta tctctattaa gggggatttc aggcttaaaa cgctctttaa
     1801 ttcaaatgct tatattagct atttctttgg aagtctttgc attggttagt ccattcttta
     1861 tgcaatgggt aatagaccat atcattgtaa ctgctgataa aaatttatta ctgaccctta
     1921 ctttgggatt tggtttactg gctatcctgc aacagttaat tagcctgtta caagcatggg
     1981 taggtatgca cctatctaca actcttaatt tacaatggaa agccaatata tttaaaaggt
     2041 tacttgactt acctaatgac tatttcagta aacgacattt aggagatgtg atttcaagat
     2101 ttggttcaat agatcatatc caagaaacac taacttctac tttttttgtt ttagttttaa
     2161 atagcttaat ggctattttt actttcgtgt taatgacaat ttacagcact caattatcgc
     2221 tgattgttct tttaacactt gttttgtaca tactaattcg ttggcttgca tattacccat
     2281 taagaaatgc aacagaagaa aatattgttc atgaagccaa acaaaactca tatttcatgg
     2341 aaaccattcg tggtatccaa tcagttaaat tatttgataa acattatcaa agacatggca
     2401 cttggatgag cctatttgtg aatacagtca ataccaagct gacaacagat aaactctctg
     2461 ctttatttga attttcaaat aaactgttgt ttagcatgga aaatattatc ataatttatc
     2521 ttggtgcaag cgcaatttta gatggttcat ttacagtcgg tgttctgatg gcttttttgg
     2581 cttataaagg gcaatttgaa agcagaacag cttctctcgt tgaccaatac atccaaatca
     2641 aaatgttagg gcttcatgct gaacgtttgg ctgacattac tttaaatgaa acagaaactg
     2701 aaattattaa gtataatcat atacctaaat tagataatga acaactggtt cttaaagttg
     2761 aaaacgtctc attcagatat gctgataatg agccatatct ttttgaaaac attaatttgg
     2821 aattcaaaga taatgaagca gttgttttaa caggacaatc tggtcggggg aagtccactt
     2881 tgttaaacat tttaacaggt agcctaaaac ctgaaactgg tacagttagt attaatgggc
     2941 atgatatata tcaagtttct ccatccttta ttaggggatt gagcgggatt gttcgccaag
     3001 atgatgtcct ttttgcaggt tctattgggg aaaatatttc attttttgat gaaagcccaa
     3061 atatggagct cattgaacaa tgtgcaaaaa tggcacaaat acatgacgat atacttaaaa
     3121 tgccaatggg ctatgagacc ttgattggcg atatgggaaa tatcttatca ggtggacaaa
     3181 agcagagagt tatcttggct cgtgcattgt ataaacgacc caaaattcta tttttagacg
     3241 aagcaagtag ccatttagat gtagaaaatg aacaaaaaat taaccataac ctaaaaagtc
     3301 ttggtattat gaaaataatg gttgcacacc gccaagaaac aattcaatcg gcagataaaa
     3361 ttctgaattt aggttgaaca gaacaagact tcatttttct ttaacaaa
//
LOCUS       DAWTHD010000141         3344 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWTHD010000141 DAWTHD010000000
VERSION     DAWTHD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.141"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(265..510)
                     /locus_tag="WM704_001988"
     CDS             complement(265..510)
                     /locus_tag="WM704_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341331.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            622..1050
                     /locus_tag="WM704_001989"
     CDS             622..1050
                     /locus_tag="WM704_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFA9341332.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVLGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1062..1538
                     /locus_tag="WM704_001990"
     CDS             1062..1538
                     /locus_tag="WM704_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFA9341333.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(1568..2056)
                     /locus_tag="WM704_001991"
     CDS             complement(1568..2056)
                     /locus_tag="WM704_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFA9341334.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKVYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(2124..3179)
                     /gene="aroG"
                     /locus_tag="WM704_001992"
     CDS             complement(2124..3179)
                     /gene="aroG"
                     /locus_tag="WM704_001992"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFA9341335.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
BASE COUNT          729 a          883 c          838 g          894 t
ORIGIN      
        1 gggaaaaaca aaaaacccct ccgccgtcat tcccgcgaaa gcgggaatct agaattttaa
       61 tgcggcaaga atttatcggg gatggccggg gtttaaaggt ctgggttccc gcctgcgcgg
      121 gaatgacggt ttagaaatca cccgaaacga gaaaaagaac cgaaaccgga caagtcggat
      181 tcccgcgcgg gcgggaatga cgataagcgt aatttcaatc cgccatattg tcttttcact
      241 tgcctgaaaa cagatttcag ccgcctatcc ctgcatccgg cggctgatgt agtcggcaat
      301 caaatcccgc ttttcccaaa aaaacttctg cattttttgt tcggaataca gtttgcggac
      361 cagagtgggc agaccgctgt ggacgatggc ggcgatttct ttgttgcctt cttcttcctg
      421 cattgccgtc tgaaaatcca aacccctgcc tttcagatat gctgcaaggc ggttgcggat
      481 gtgtgaattg agttcgatgg atttgagcat tttgcctccg ttttgcggta aggatttatt
      541 cgatttgttt atccgcctga ttttatcacg tttcccaagc ggtttgcctg tacaataccg
      601 cttttcggaa gggtgtgggc gatgatgttt tcttggttca agctgtttca cttgtttttt
      661 gtcatttcgt ggtttgcagg gctgttttac ctgccgagga ttttcgtcaa tatggcgatg
      721 attgatgcgc cgcgcggcaa tcccgagtat gtgcgcctgt cggggatggc ggtgcggttg
      781 taccgtttta tgtcgccttt gggtttcggc gcggtcgtgc tcggcgcggc gataccgttt
      841 gccgccggcc ggtggggcag cggctgggtt cacgtcaaac tgtgtttggg cttgatgctc
      901 ttggcttatc agttgtattg cggcgtgctg ctgcgccgtt ttcaggatta cagcaatgct
      961 ttttcacacc gctggtaccg cgtgttcaac gaaatccccg tgctgctgat ggttgccgcg
     1021 ctgtatctgg tcgtgttcaa accgttttga cggagcatct tatgccgata gaaatcgaac
     1081 gccgtttttt gattgaaaac gacaaatggc ggcaatacgc cgatgagccg ctgctgctga
     1141 aacagggcta cctgtctgtt gaaaaagaac gcaccatccg catccgcatt gccggaaaac
     1201 gggcgtggct gacgctgaaa ggctatattt cggaaatcag ccgcagcgag ttcgaatacg
     1261 aaatcccgct tgccgatgcg gaaaagatga tggaaacgat gtgcccgttt aagatggaaa
     1321 aacggcgtta tccggtcaga tggggcggca gcctgtttga agtcgatgtt ttccttggcg
     1381 aaaactcgcc tttggtcgtc gcggaaatcg agttgcccgc cgaaaatgcc gattttgacc
     1441 gtccggactg gctggggcgc gaaatcactt cagacggtat gtttaccaat gcctatttga
     1501 gcaaacatcc gttctcaagc tggaaaaatg ccgtctgaag cggcttcaga cggcatctgc
     1561 cttgcggtta tcccttgagg taatgcacga atgtatatgc aacgcctttg ctgctgacac
     1621 ggcgttccgt ccgctctgct tctttccaat gcgtccggtc tatttctggg aaaaatgcat
     1681 ctccttccac agacaaatcc acttcggtta tccgcaaatc ggtcgctaat ggcatcgctt
     1741 gtccgtatat ctgcgcgccg cccataatga cggcttcttc cgcgccggcg cacaatgcca
     1801 atgccgcctc caaacttgcc accgtttccg cgcctgccgc gcaataatcc gcctgccggc
     1861 tgatgacgat gttcctccgt ccgggcaggg gtttgacggg cagggattcc cacgttttcc
     1921 gccccataat gacgggtttg cccaaggtat agactttgaa aaatgcgaaa tcttcgggga
     1981 tgtgccaagg catagcattg cccgccccga tgcacaggtt ttccgcacac gccgcaatta
     2041 tggttatttt gagcatattt tctccacaaa aatgccgtct gaacgcttca gacggcattt
     2101 tatgacattc tgcgtcaaaa atctcaacct gcgcgcgcca tacgttttgt gttcgcaccc
     2161 gccaacaatg ccaacaattc ttcagtcgcg ccccaaccga tacacgcatc ggtaatgctc
     2221 ttgccgtaca cttccggctt gtcctgcctg ccttcgacca aatggctttc caccatcacg
     2281 cccatgatat tgccgccgtc ctgttccaat tggtcggcaa tgtcttgtgc cacttccatc
     2341 tgccgggtgt aatccttgcg gctgttggcg tggctgcaat cgatcatcag cttgtcggtt
     2401 acccctgccg cacgcagttg ttccaccgcc tcgctgacgt gtcccgcatc ataattcggc
     2461 tcttttccgc cgcgcaaaat gacatgacag tcgggattgc cgccggtatg gacaatggcg
     2521 gaatgcccgg ccttggttac agacaagaaa tgatgcgaat ggctcgccgc accgattgcg
     2581 tcgatcgcaa tcttcaaatt gccgtccgta ccgtttttaa agccgacggg gcaggacaga
     2641 ccgcttgcca attcgcggtg gacttggctt tcggtcgtcc gcgcgccgat tgccccccaa
     2701 gaaatcaggt cggcataata ctggggtgta atcatatcca aaaattcggt agaggcaggc
     2761 atgcccatat tattcaggga caacaacagg ctgcgcgcct gacgcaaacc gaaattgatg
     2821 tcgaacgtac cgtccaaatg cgggtcgtta atcaaacctt tccaacccac cgtcgttctc
     2881 ggcttctcga aataaacgcg catcacgatc aaaagctcgt tttcataccg cttgcggagt
     2941 ttcaacagac gttccgcata ttccaacgcc gctttcggat cgtgaatcga acacggcccg
     3001 ataatgacca acagccgcct gtccctgccg tgaaccaaat cggaaatttc ctgacgggtg
     3061 cggtgaacca agcccgaagc ctctttggaa atcggcagct cgtaaagatg ggcaatcggc
     3121 ggcaacaact ctttaacttc tttaatctta atatcgtctg tggggtaatg gtgtgtcatc
     3181 ttgcatcttt cctgatgggt ttttatacgc ggcttaggtt acatcgttta ccttgcacaa
     3241 acaagtgcaa catattacta caaagctatt ttatttcaaa ataactgata tatctttttt
     3301 catagatttt aaatttcata tttttaatat tttaggaatt ttat
//
LOCUS       DAWTHD010000142         3305 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWTHD010000142 DAWTHD010000000
VERSION     DAWTHD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.142"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(63..800)
                     /gene="ubiE"
                     /locus_tag="WM704_001993"
     CDS             complement(63..800)
                     /gene="ubiE"
                     /locus_tag="WM704_001993"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFA9341336.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(843..1211)
                     /locus_tag="WM704_001994"
     CDS             complement(843..1211)
                     /locus_tag="WM704_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFA9341337.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            1331..2950
                     /gene="mnmC"
                     /locus_tag="WM704_001995"
     CDS             1331..2950
                     /gene="mnmC"
                     /locus_tag="WM704_001995"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFA9341338.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            complement(2947..3096)
                     /locus_tag="WM704_001996"
     CDS             complement(2947..3096)
                     /locus_tag="WM704_001996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341339.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT          856 a         1094 c          668 g          687 t
ORIGIN      
        1 aaaaaataca aaaatgccgt ctgaaacgtg ttcagacggc attggcttca caggcagtcc
       61 gtttaaaatt tcacgccctt atgcagcgcg acgatgcccg cactcatatt gtgataatcc
      121 acgctgtcga agcctgcatc cagcatcatc tgtttcaaag tttcctgatc ggggtgcata
      181 cggatggatt cggcgagata ctggtagctg tccgcatctt tggcaatcag cctgcccatt
      241 accggcaaca gcttgaaaga ataaaaatcg tatgcgcctt cgagcggctt gtagattttg
      301 gaaaactcca acaccagcaa cgtaccgccc ggtttcaaaa cacggtacat ttccttaagc
      361 gcggcatctt tatacgtcat gttccgcaag ccgaacgcca cggagaccaa gttgaaataa
      421 ttgtcgggga aaggcagttt ttccgcatcg gcaagcgaca cgggtaaaat catgccttcg
      481 ttcaacagac ggtcgcgccc gacggtcagc atagaagaat taatatcggt cagccaaacc
      541 tcgccttcct tgccgacccg tttcgcccaa ccgcgcgaca aatcgcccgt accgcccgca
      601 atgtccaaca ctttatcgcc ttttttcagg tgcgccgtat ggatggtaaa atgcttccat
      661 acgcggtgca gccctgccga catcacatca ttcataatgt cgtagttttt ggcgacggag
      721 tgaaacactt ccgccacttt tccggctttc tcatcttcgt tgaccgtgct gaacccgaaa
      781 tgcgtttttt gtccgcccat attctaacca cctccctgat accgccctac ggcatcccaa
      841 acttaacaat ctgattgctt ttcaaaacgc gacgcgcccg ccgccgccaa tttgtccaaa
      901 tattcctgcc acatcgcatc gtatccgtat gccagtctgt gcagatacgc ccaatcgtaa
      961 agaccgctgt cgtgcccgtc cgaaaaactg attttcagcg catactgtcc gacaggctgc
     1021 aaatccgtaa tctgcacatc cgccttaccg gtctgcaaaa catcctgtcc cacgccgtgt
     1081 ccgcgcactt ccgcactcgg cgaatagacg cgtaaaaatt cggcaggcag atttttaggt
     1141 tcgtccccat aagtcaaaac caacacggca cggtttttca acaaacggat ttcaacagga
     1201 atattattca aaaaacacct cggttcggca tacaatatcc ggcaggcgta cgcgtgcctt
     1261 taaacggcgc gtattttaac agctttatcc cgataccgat attcaaaccc acacttcaga
     1321 cggcattgat atggacaatc tcgtatggga cggcattccc gacatccgca cactcgacca
     1381 aaccatccgc aaacacgcac acccgctcaa cctgattgtc tgcctccccg ataatcagat
     1441 tcccgatttt caaaccgcac aagatgcttc ggactcggaa tgccgtctga agcaccgttt
     1501 ggatcaggca acccagtgcc tccagttcga cagcatcaac ctcatcgaac acatcctgcc
     1561 cgatgtccgc ttctggctgg ttcccccttc acgcacccgc cgcctgcacg aacacttcca
     1621 ccacatttcc tggcagaccg aagccatccc gcaaaccgaa agcaagtccg acaaaccctg
     1681 gtttgcactt ccacaaacat ccgaacggaa aaaaccggaa cacgtcctcg tcatcggtgc
     1741 aggcattgcc ggcgcatcga ccgcccacgc cttagcatca cacggcattt ccgttaccgt
     1801 attggaagcc cgaaaagccg ctcaagccgc cagcggcaac cggcaagggc tgctttacgc
     1861 caaaatctcg ccgcacgaca ccggacagac cgaactgctg cttgccggct acggctacac
     1921 caaacgcctg ctcggacaca tcctgcccga ctccgacact tggggcggca acggcatcat
     1981 ccacctcaat tacagccgca ccgaacaaca acgcaatcac gaattgggtt tgcaaaaaca
     2041 ccataaccac ctctaccgca gcatcacgtc tgcagaagcc gaaaaaatcg ccggcatccc
     2101 gctgaacacg ccctacgccg aaccattatg cggactctac tggcaacacg gcgtatggct
     2161 caatccgccc gcattcgtcc gcaccctcct cagccatccg ctgatcgaac tatatgaaaa
     2221 cacaacgtta accggcattt cccacgacgg agaaaagtgg attgcaagca cgccaaacgg
     2281 cacatttacc gccacacaca tcatctactg caccggcgcg cacagcccct gcctgcccga
     2341 aaccaacctc gccgccctac ccctcaggca aatacgcgga caaaccggac tcacaccgtc
     2401 caccccgttt tccgaacaac tgcgttgcgc cgtttcaggc gaaagctaca tcagcccgtc
     2461 gtggcacgga ctgcactgct acggcgcgag ttttattccc aacagcagca ataccggatg
     2521 gaacgaagcc gaagaagcct caaaccgcca agcattggca caccttaacc ccgcccttgc
     2581 cgaatcattg tttgccgcca acccaaaccc ccaaaaacac caagggcacg ccgccatacg
     2641 ctgcgacagc cccgaccacc tccccgtcgt cggcgcactc ggcgacattg ccgccatgcg
     2701 gcagacttac gccaaacttg cactggacaa aaactaccgc atcgacacca catgcccata
     2761 tctgcccaat gcctacacca ataccgccca cggcacacgc gggcttgcca ccgcccccat
     2821 ctgcgccgcc gccgttgccg ccgaaatcct aggcttgccc catctctttt cacaacgcct
     2881 gcgccacgcc ctacacccca accgcaccgt catccgcgcc atcgtcagaa ggcagaacct
     2941 gattccttaa gcctttgccg catgccgtcc cgaccacaaa aaatcatatt caaataaaca
     3001 taccaatcct gacggtaaaa tatcgaaaac acccataacg ttttcttccc aaaataagga
     3061 gtaaggaatg cctaacaaat tgcctttgct gtccaagatg aattttccgt aacattcgaa
     3121 gacttcctta accctgccag ccgcttcggt ttctgtaccc cctgttagat aaggaaggga
     3181 tgaaaaagcc ctgttacttg tatgaagcaa agggcgttaa tttttgttaa tcttcccttc
     3241 tcaggaactc aatatataag gatgttaatt tttatttccc ttctcgagga ctcaatagta
     3301 tgaga
//
LOCUS       DAWTHD010000143         3296 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWTHD010000143 DAWTHD010000000
VERSION     DAWTHD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.143"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            248..466
                     /locus_tag="WM704_001997"
     CDS             248..466
                     /locus_tag="WM704_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341340.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(483..842)
                     /locus_tag="WM704_001998"
     CDS             complement(483..842)
                     /locus_tag="WM704_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341341.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(843..1382)
                     /locus_tag="WM704_001999"
     CDS             complement(843..1382)
                     /locus_tag="WM704_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HFA9341342.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1542..2246)
                     /locus_tag="WM704_002000"
     CDS             complement(1542..2246)
                     /locus_tag="WM704_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9341343.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
     gene            2401..2589
                     /locus_tag="WM704_002001"
     CDS             2401..2589
                     /locus_tag="WM704_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9341344.1"
                     /translation="MSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKRLYKDVMLLT
                     KNKVSISELISEFRVCPK"
     gene            2669..2824
                     /locus_tag="WM704_002002"
     CDS             2669..2824
                     /locus_tag="WM704_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341345.1"
                     /translation="MKKQDRNRLSKKDRRLIKKAMLKAAAKGSDEVYRIAPGLKDGFE
                     LLGKQPD"
     gene            complement(2801..2989)
                     /locus_tag="WM704_002003"
     CDS             complement(2801..2989)
                     /locus_tag="WM704_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341346.1"
                     /translation="MPSDLSFILTKELIRSGSIRLKGSTAKGQAGELAVFIRTLHQKP
                     EESEPNTDNEYLIGLLSK"
     gene            3162..>3296
                     /locus_tag="WM704_002004"
     CDS             3162..>3296
                     /locus_tag="WM704_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341347.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     R"
BASE COUNT          823 a          734 c          874 g          865 t
ORIGIN      
        1 tgatataata aactcataga ttaggaaatg aagaaacccc gtatcagccg atacggggca
       61 acctgaaaga gaaaggaggc aatgtgatta agaaaatcct aatctgtgtg attttgtatt
      121 tccttgcaag ctccccagcg tggtagggaa atacaaaaca agtgattaac gattaaccat
      181 ttgcagggca gttaaggagg ctgcccttcc tcttatcttt cccatactct actataagga
      241 tttaaaaatg gcaaattcca acagcgggca ttctaaaaaa ttgcgcgcgg caacggcggc
      301 ggcggcgaca aaggcaaaac tggcaagcgg cgaataccgg cagttttcag tgcaggggcg
      361 tgccgaagac gtggagctta tccttgccgc cgtggaaaaa gccgggggca gccgcgtaca
      421 ggctttggca aagatttgca ggcggtatct cgaggggctg tcttaagggg ggtaagacaa
      481 ggttatttgg gaatatcggg caggataaaa ggcgggtgtc tgtcaaaacc gatgattttg
      541 ataaagccgt cgcaaagggt atagtgaagg atgtaggcgg atgtcaaatc gccatacctg
      601 ccgccgttgt atttgggaat gccgatgtga tagtgccaaa ggttgtgccg ttgggcaaat
      661 cggacttttt ccagccattg aggatcgtct gccggtacgt tgtcgctgga tttgttcctg
      721 ccgggtaacc ctgataagcc gttctgttgg acgtgtgcga taaattccgc aatttttctg
      781 cggtcttctt tggggtagtt tttcaacgct cttttaaagt cttcgccaag cagcacctgc
      841 atttactgct ccaaccattt ttccaagtcg tcggcgccgt tgaaatgcgg gacgctgaca
      901 aagctgtctt caacggctgt tttgagtttt tcaagattga aattgtaatc cgtctgcggc
      961 agcgcatggc ggaaatattc ccccatcagc gcaatcccta tgacttcgcc ctgccgggtc
     1021 tgtatccacg gcgcttcctg atgggtttta ttgcgtaatg cccaagcggt gtagcagccc
     1081 tgctctttac ggacacggtt gaggacaacc aactcttcgt ccgcatattt gtccggttcg
     1141 atatgggccg caggcaatgg gctgccgccg tattttttgt aggtgcggta aatgcagggg
     1201 actaccggac cgtgctgcca gtgttcgata ttttcggcaa acagggggcg gttaagtatg
     1261 gcaagggcgt agccttgtgc gtaatacagg agtttttgaa gtttgagatt ggagatttgc
     1321 tccccgtcct cttcttcaaa aggggaaagg aagaaatctg ccacgtcgta cgcgttcagc
     1381 attttctgtt tccgttgtta tttttctgta tattaaggcc gccggcccat tatttcaaga
     1441 aataatttgt gcaaattatc cggtatcggc aggctgtccg cgccgtcggc atatcgggcg
     1501 gatatatttg aaaaatactt gccctgccgt ttcagacggc atcaatccaa cacgctccac
     1561 caaaataccc tgccgataac ggacaggctg tccaaagggg cggtttcgtc gggatagaag
     1621 ccgctgttgt ggctgcgtat cagcacgctg ttgccgggct gccgtatcag gtatttgacg
     1681 cggaacatgc cgtcttgggc gaaggcgtat attttgccgt cgcgtatggc ggtttcgccc
     1741 gtatctacgg cgattgccgc gtcttctgcg attttttcct ccatgctgtc gccggtcagg
     1801 gtgcagcaga agacgttgtc ggggttgatg cctttgcgcc ttaacgttga tttgccgaac
     1861 ggcaggcggt agccgttgta gtcggggatt tcatacgcgc ccgccccgcc tttgaagcag
     1921 ctctctttga ggtaggggac gaaaacataa tcatcgtcgg gcagcgggtc gttactgctc
     1981 cacatcatcg ggcggtggat gtctttgact tcgtgcggca ggtcggggtt gatgaggacg
     2041 ggcgttggtt cgctcggaac ggaatccaag tacagactgc ccatcccata ttcttcttca
     2101 agccgtctgc ttaccttttc gccaaaagat gctttaccgc tcatcaattg agataaaagg
     2161 cttttgtctt taaccggcaa ctttttattt tcaaagaatc tttttaaatt atcgatacgg
     2221 atggccttca gatctctatt agtcattttc gcgctccatt cagattggga ttcaatttta
     2281 tttagtttct gctaaacaaa caaatattaa attttcttgc ctatgcgttt aatatttgct
     2341 aaactcatta aatatttacg gtgaaactac agaatgactc attcagaatt tattaaaacg
     2401 ctgtcagaac agcgtggtgc aaaaacggaa tacgctaaaa aacttggctt atccctgtct
     2461 tttctatggc agatagaaaa cggacgggca gtaacgccaa agcgtcttta taaagacgtt
     2521 atgttgttga caaaaaataa ggtttctatt tctgaattaa tttcggagtt cagggtttgt
     2581 cccaaataaa aagcccgtcg ggtcagatgt taattgatat aaccgaattg aagcggaagt
     2641 catccgcaat ttaccggaaa gaaaaaaaat gaagaagcag gacagaaacc gcctgtcgaa
     2701 gaaagacaga cggctgatta aaaaggcgat gctgaaagcc gccgccaaag gcagcgatga
     2761 ggtttacaga atcgcgccgg ggttgaaaga cggctttgaa ttacttggaa agcagcccga
     2821 ttaaatattc gttatcggta tttggctccg attcttcggg tttttgatga agtgttcgga
     2881 tgaataccgc caattctccg gcttgtcctt tggccgtact gccctttaag cggatagaac
     2941 cgctgcggat aagctctttg gtaagtatga aggataagtc ggacggcatt tttttactcc
     3001 gtcggccgtt gtgtggaaac ccggttgcaa cggggtgacg gcaaatcgga aagacggctg
     3061 accgcccgga cagacgggcg gccgataaag aaaaacccgc acggggcggg taatccccct
     3121 gaattgcagg gaagcggttc aggtaacggc gaaaggcgat tatgaatcaa aaacaaacgc
     3181 aatgcaaaca aattgtcgat tacatccgta acaagggatg catcacatcc cttgaggctt
     3241 atcagaacct gaaggtgacg cagcttgcgg cacggataac cgacttggaa ggcagg
//
LOCUS       DAWTHD010000144         3265 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWTHD010000144 DAWTHD010000000
VERSION     DAWTHD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.144"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            295..>3265
                     /locus_tag="WM704_002005"
     CDS             295..>3265
                     /locus_tag="WM704_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9341348.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAMAETREYAIIMNGR
                     NQPEVKSNVPSSIKDKDRMREYTYHKGSRGGGGSVSFNNNDELVSQQSGTAVFGTATY
                     LPPYGKVSGFDTDRLKKRNNAVDWIRTTRIALAGYSYAGIHCGSISGCPKLVYKTRFS
                     FGQQGLQRKGNKLDIYEDKSRENSPIYKLSDYPWLGVSFNLGAEGTAKDGKVTNKLVS
                     SFSEDNNNQTIVSTTEDSPISLGDSRREHTAVAYYLNAKLHLMDKKKIENIAPGKTVQ
                     LGILKPSIDVRTRNTGFGLLNFWAKWDIKDNGQIPVKLGLPEVKAGRCINEPNPNKNT
                     QAPSPALTAPALWFGPVQNGKVQMYSASVSTYPGSSSSKIFLQNLSRNDDKNKPGRYS
                     LVTLNENDIKSRQPSFNGRQTVIRLDDGVHLIRLSRSKDEVVTFGNNGNNGTFGIVKE
                     ANVNLEADEWKKVLLPWTVRGPDNDNKFKSINREPGKYSQRYRIRENGNRDLGDIVNS
                     PIVAVGGYLATAANDGMVHIFKKNGGSDERSYNLKLSYIPGTMPRQYFDNDTSALKNS
                     TLAQELRTFAEKGYVGDRYGVDGGFVLRQVNLNGQDRVFMFGAMGLGGRGAYALDLSK
                     INGNYPAAAPLFDVKNGDNNGKNGKNRVELGYTVGTPQIGKTQNGKYAAFLASGYAAK
                     NIGSGDNKTALYVYDLGNTLGTPIAKIEVKDGKGGLSSPTLVDKDLDGIVDIAYAGDR
                     GGNMYRFDLSNSDPSKWSVSTIFEGGKPITSAPAVSRLADKRVVIFGTGSDLSEEDVV
                     GKDQQYIYGIFDDDKPTVKVTVQNGMGGGLLEQNLTQENKTLFLSNNKASGGSADKGW
                     AVKLTGGQRVTVKPTVVLRTAFVTIRSYTGMDKCGAQTAILGINTADGGALTPRSARP
                     IVPEANTAVAQYSGHKTTSKGKSIPIGCMEKGGKTVCPNGYVYDKPVNVRYLDETETD
                     GFSTTADGDAGGSGIDPA"
BASE COUNT          897 a          830 c          857 g          681 t
ORIGIN      
        1 aaggcgggct gtagggtagg cttcatcccg ccaatctcac tgaatccgtc aatttccgca
       61 attcaattaa ataccgtcaa accgatgccg tcattcccgc gcaggcggga atccggaccg
      121 gtcgggcatc tgcggcggtt tgctaaaaaa cgctttaccg tgataagtgc gcaaagttaa
      181 aatggggagg taagcttttc aatcagcaat tcggcgggcg cggaatcggg cggtttaccg
      241 aaccccggcg ttcgcggcgc gccgtcccgc gaaggcaaac ttaaggaata aaatatgaat
      301 aaaactttga aaaggcaggt tttccgccat accgcgcttt atgccgccat attgatgttt
      361 tcccataccg gcgggggggg ggcgatggcg gaaacccgtg aatacgctat tatcatgaac
      421 gggcgaaacc agcccgaggt aaagtcgaat gtgccatctt caataaagga caaagacagg
      481 atgcgcgaat atacttatca taagggctca cgaggaggag gaggctctgt ctcattcaac
      541 aataacgatg agcttgtttc tcaacaaagc ggtactgccg tttttggcac agccacctac
      601 ctgccgccct acggcaaggt ttccggtttt gataccgata ggctgaaaaa gcgcaacaat
      661 gccgttgatt ggattcgtac cacccgcatc gcgctggcag gctacagcta cgccggtatc
      721 cattgcggaa gtatctcagg ctgtcccaaa cttgtctata aaacccgatt ttccttcggt
      781 caacaagggt tgcaaagaaa gggcaacaag ctggatatat acgaagacaa aagccgcgaa
      841 aattcgccca tttacaaatt gtcggattat ccttggttgg gcgtgtcttt caatttgggc
      901 gccgagggta ccgccaaaga tggtaaggta accaacaaat tggtatcttc ttttagtgaa
      961 gacaataata atcaaaccat cgtctctacg acagaagact cccctatttc ccttggcgac
     1021 agtcggcgcg aacataccgc cgtggcctat tatctgaacg ccaaactgca cctgatggat
     1081 aaaaaaaaga ttgaaaatat cgcgccaggc aaaacagtgc agttgggtat cttgaagccg
     1141 agcatcgatg tgcggacaag aaatacgggg ttcggcttgc taaatttttg ggctaagtgg
     1201 gacattaaag ataacgggca gattccagtc aagctcggcc tgccggaagt caaagcaggc
     1261 cgctgcatca acgaaccgaa ccccaataag aatacccaag ccccttcgcc ggcactgacc
     1321 gcccccgcgc tgtggttcgg ccctgtgcaa aatggtaagg tgcagatgta ttccgcttcg
     1381 gtttctacct accccggcag ttcgagcagc aaaattttcc tgcaaaacct ttcccgcaat
     1441 gatgacaaaa acaaacccgg ccgctattcc ctcgtaacct tgaatgagaa tgatattaaa
     1501 agtcgacagc cgagtttcaa cgggcggcaa acagtcatcc gattggatga cggcgtacat
     1561 ttgatcagac tgagtagaag caaggatgag gtcgtcactt ttggaaacaa cggcaacaac
     1621 ggcactttcg gcattgttaa ggaagcgaac gtcaatcttg aagccgacga gtggaaaaaa
     1681 gtgctgctgc cttggacggt tcggggtccc gataatgaca ataaatttaa atcaattaac
     1741 cgagaaccag gcaaatacag ccaaagatac cgcatccgcg aaaacggcaa tcgcgatttg
     1801 ggcgacatcg tcaacagccc gattgtcgcg gtcggcgggt atttggcaac cgccgcgaac
     1861 gacgggatgg tgcatatctt caaaaaaaac ggcggcagtg atgaacgcag ctacaatctg
     1921 aagctcagct acatccccgg cacgatgccg cgccaatatt ttgataacga cacttccgct
     1981 ctcaaaaact ccaccctcgc ccaagagctg cgcacctttg ccgaaaaagg ctatgtgggc
     2041 gaccgctacg gcgtggacgg cggctttgtc ttgcgtcaag ttaacttaaa cggacaagac
     2101 cgcgtgttta tgttcggcgc aatgggcctt ggcggcagag gcgcgtatgc cttggattta
     2161 agcaaaatca acggaaatta tccggccgcc gctcccctgt ttgatgtcaa aaatggcgat
     2221 aataacggca aaaacggcaa aaatcgcgtg gaattaggct acaccgtcgg tacgccgcaa
     2281 atcggcaaaa cccaaaacgg caaatacgcc gccttcctcg cttccggtta tgcggctaaa
     2341 aatattggca gcggcgacaa taaaaccgcg ctgtatgtgt atgatttggg aaacacctta
     2401 ggtacgccga ttgcaaaaat cgaagtgaag gacggcaaag gcgggctttc gtcccccacg
     2461 ctggtggata aagatttgga cggcattgtc gatatcgcct atgccggcga ccggggcggc
     2521 aatatgtacc gctttgattt gagcaattcc gatcctagta aatggtctgt aagcactatt
     2581 ttcgaaggcg ggaagccgat tacttccgcg cccgccgttt cccgactggc agacaaacgc
     2641 gtcgtcatct tcggcacggg cagcgatttg agtgaagagg atgtagtcgg taaggatcaa
     2701 caatatattt acggtatctt tgacgacgat aagccgacgg ttaaggtaac ggtacaaaac
     2761 ggcatgggag gcgggctgct cgagcaaaac cttacgcagg aaaataaaac cttgttcctg
     2821 agcaataata aggcatccgg cggatcggcc gataaaggct gggcggtgaa attgacaggc
     2881 ggacaacgcg ttaccgtcaa accgaccgtg gtattgcgta ccgcctttgt caccatccgc
     2941 agctatacgg gcatggacaa atgtggcgcg caaaccgcca ttttgggcat caataccgcc
     3001 gacggcggcg cattgactcc gagaagcgcg cgcccgattg tgccggaagc caatacggct
     3061 gtcgcacaat attccggcca taagacaacc tccaaaggca aatccatccc tataggttgt
     3121 atggaaaaag gcggtaaaac cgtctgcccg aacggatatg tttacgacaa accggttaat
     3181 gtgcgctacc tggatgaaac ggaaacagac ggattttcaa cgacggcgga cggcgatgcg
     3241 ggcggcagcg gtatagaccc cgccg
//
LOCUS       DAWTHD010000145         3259 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWTHD010000145 DAWTHD010000000
VERSION     DAWTHD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.145"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            88..924
                     /locus_tag="WM704_002006"
     CDS             88..924
                     /locus_tag="WM704_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9341349.1"
                     /translation="MNNASSEKTIEFAGRSWTKTADSLPQKAHGLRYGLGVSREINAG
                     GHHFLYGGISRGVHYWDNKDFSEQSLRLSFGHQNRSVTRSFGTVPFVGQNLLGGSRYN
                     FAGGFNADFSRRLGERWRLTLNAGNMWKHYQEDRTAARYGSHMPLAGATLMYSAPKDR
                     LLYGGADWPHDMTKEAEQASVRKGLRVGAVKTFDGGLGLRANLRYTRRTFDAPGTIVY
                     RFPRKDHEYQANLSLRHDKISWKGFTPQLNFRYLKIDSNMKSFYTRKNTQIFMSVEKD
                     FK"
     gene            1087..2595
                     /gene="ppx"
                     /locus_tag="WM704_002007"
     CDS             1087..2595
                     /gene="ppx"
                     /locus_tag="WM704_002007"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFA9341350.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDSKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(2805..3149)
                     /locus_tag="WM704_002008"
     CDS             complement(2805..3149)
                     /locus_tag="WM704_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341351.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAT
                     QEAADKMKDAAK"
BASE COUNT          762 a          946 c          834 g          717 t
ORIGIN      
        1 aaggtgctga agcaccgggc ggatcggttc cgtaccatcc gtactgcctg cggcccgccg
       61 ccttgtcctg atttttgtta atccgctata aacaacgctt cgtccgaaaa aacgattgaa
      121 tttgcgggca gaagctggac gaaaaccgcc gacagcctgc cgcaaaaggc acacggtttg
      181 cgctacggct tgggcgtgtc gcgcgaaatc aatgcgggcg ggcatcattt cctctacggc
      241 ggcatcagcc gcggcgtgca ttattgggat aacaaagatt tcagcgaaca gagcctgcgc
      301 ctgtcgttcg gccatcaaaa ccgttcggta acgcgctcgt tcggcaccgt gccgtttgtc
      361 gggcaaaacc tcttgggcgg cagccgatac aatttcgccg gcggcttcaa tgccgatttc
      421 tcccgacgct tgggcgaacg ctggcggctg acgctgaacg cgggcaatat gtggaagcat
      481 tatcaggaag accgcaccgc cgcccgatac ggcagccata tgccgctggc gggcgcgacg
      541 ctgatgtatt ccgcgccgaa agaccggctg ctttacggcg gtgcggactg gccgcacgac
      601 atgacgaaag aggcggaaca ggcttccgtc cgcaagggtt tgcgtgtcgg cgcggtcaaa
      661 acgttcgacg gcggcttggg gctgcgggca aacctgcgct atacccgcag gacttttgat
      721 gcacccggga caattgtgta ccgcttcccg cgcaaagacc acgaatatca ggcaaacctg
      781 tcgttgcggc acgacaaaat ctcttggaag ggctttacgc cgcaactcaa tttccgctat
      841 ctgaaaatcg acagcaatat gaaaagtttt tacacacgca aaaatacgca gattttcatg
      901 agcgtggaaa aggatttcaa ataagcgcaa aaaatgccgt ccgaacgttt ttcagacggc
      961 attttttgct gtcggaaaca taccgtcggc aacatccgtg agcagaatca aaaacagccg
     1021 catcatttat tgtcaacgcc tgcgccgtca gagtaacatt gcgtttttcc cccaccggta
     1081 tccgccatga ccaccaatcc cgcaaacgtc ctcgcctccg tcgatttggg ttccaacagt
     1141 ttccgcctcc agatttgcga aaacaacaac ggacaattaa aagtcatcga ttccttcaaa
     1201 cagatggtcc gttttgccgc cggtctggac gaacagaaaa acctcagcac atcctcgcag
     1261 gaacaggctt tggactgcct ggcaaaattc ggcgaacgcc tgcgcggctt ccgccccgag
     1321 caggtgcgcg ccgtggcaac caatacattc cgcgtcgcca aaaacatcgc agatttcctt
     1381 cccaaggccg aagcggcact gggtttcccc atcgaaatca tcgccgggcg cgaagaggcg
     1441 cggctgattt ataccggcgt gatccacacc ctccccccct gcgggggcaa aatgctggtt
     1501 atcgacatcg gcggcggttc gacagaattt gtcatcggtt cgacgctgaa tcccgacatt
     1561 accgaaagcc tgcccttggg ctgcgtaacc tacagcctgc gcttcttcca aaacaaaatc
     1621 accgccaaag acttccaagc cgccatttcc gccgcccgca acgaaatcca gcgcatcagc
     1681 aaaaatatga ggcgcgaagg ttgggatttc gccgtcggca catcgggttc ggcaaaatcc
     1741 atccgcgacg tgcttgccgc cgaaatgccc caagaggcgg acattaccta caagggaatg
     1801 cgcgccctcg ccgaacgcat catcgaagcc ggttcggtca aaaaagccaa atttgaaaac
     1861 ctgaaaccgg aacgcatcga agtttttgcc ggcggacttg ccgtgatgat ggcggcgttt
     1921 gaggaaatga aactcgacag gatgaccgta accgaagccg ccctgcgcga cggcgtgttt
     1981 tacgatttga tcgggcgcgg cttaaacgaa gatatgcgcg gacaaacggt tgccgagttc
     2041 caacaccgct accacgtcag cctcaatcag gcgaaacgca ccgccgagac cgcgcaaacc
     2101 tttatggaca gcctctgtca tgccaaaaac gttacggttc aagaacttgc cttgtggcaa
     2161 cagtatctcg gacgcgccgc cgcgctgcat gaaatcggtt tggacatcgc ccataccggc
     2221 tatcacaagc attccgccta catcctcgaa aacgccgata tgccgggttt ttcacgcaag
     2281 gaacagacca tacttgccca actggtcatc ggtcatcgcg gcgatatgaa aaaaatgggc
     2341 ggcattatcg gcggcaacga aatgttgtgg tatgccgttt tgtccctgcg ccttgccgcc
     2401 ttgttctgcc gttcgcgcca agacctgtct ttcccgaaaa atatgcagtt gcgtacggat
     2461 acggaaagct gcggcttcat cctgcgtatt gacagtaaat ggctggaacg ccaccccctg
     2521 attgccgacg cattggaata tgaaagcgtc caatggcaaa aaatcaatat gccgttcaaa
     2581 gtcgaggccg tctgaacctt gacaaaccaa atgccgtcta aatgatgttc agacggcatt
     2641 tccctgtccg caatatcccg atatgcgcgg cacatcctcc ctgcacggtc atgcaggcgt
     2701 aaaaaacaag gggcatacaa ctcaaaaacc gcctgaaaat cttcaggcgg tttcatttgg
     2761 gttgccggca ggcggcatct cgtcattttt gccaaggcaa caaattattt ggcggcgtct
     2821 ttcattttgt ctgccgcttc ctgagtcgcg tcggcagctt tgttcaaagt gtctttagcc
     2881 gcttcgctta ccgcttcttt ggtttcggct gccgcgtctt tggcttcagt tacagcttcc
     2941 tcggcacttg cctttgcatc agccgcagca tctttgactt ggcctttcgc ttcttcgacg
     3001 gcagaagcgg cagactcggc ggcagaagcc gcagtgtctt taacatcgga ctcaacggct
     3061 tgggccgctt ctttaacctc ctgtttggct tcttgcgaac aagctgccaa ggcagccgcc
     3121 atcattgcgg caatcaataa ttttttcatg tcttatcctt cttgagttgt tgattaaggt
     3181 tttgcttaaa aaccggaccg tgttccatca atcggctgat tttgcccatc ggccggagag
     3241 aaaacggttt cccgtttag
//
LOCUS       DAWTHD010000146         3247 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWTHD010000146 DAWTHD010000000
VERSION     DAWTHD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.146"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            111..1067
                     /locus_tag="WM704_002009"
     CDS             111..1067
                     /locus_tag="WM704_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFA9341352.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQANLADGFI
                     FVQNLLNGEPLEECISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(1139..1468)
                     /locus_tag="WM704_002010"
     CDS             complement(1139..1468)
                     /locus_tag="WM704_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HFA9341353.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(1544..2914)
                     /gene="glmU"
                     /locus_tag="WM704_002011"
     CDS             complement(1544..2914)
                     /gene="glmU"
                     /locus_tag="WM704_002011"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFA9341354.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(2969..3241)
                     /locus_tag="WM704_002012"
     CDS             complement(2969..3241)
                     /locus_tag="WM704_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFA9341355.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
BASE COUNT          595 a          858 c         1004 g          790 t
ORIGIN      
        1 ctatatccgc catatgttgc agggcgggct ttcagaccgc cgctatcggt taatggaaaa
       61 acggcgtgca gggatgccca ccctgctgca cggatattga aggaaacgcc atgagtcaaa
      121 accaacccgc acaaccgacc aaacgcaatc tgttcaaaac cgcccttgcc gtcggcgcaa
      181 tcggcgcaat cggaggttat ttcggcggca aaaaacaggg cgaaaccgcc gaacgcaccg
      241 ccgaaagcca acactcgccc caagcctatc cctgctacgg cgaacatcag gcaggtatcg
      301 ttacgccgcg gcaggcgttt tccattatgt gcgccttcga cgtaaccgcg caaagtgcca
      361 agcagctgga aaacctgttc cgcacactga ccgcccgcat cgagtttctc acccaaggcg
      421 gagaatacca agacggcgac gacaaactcc cgtcagccgg cagcggcatt ttgggcaaag
      481 ccttcaaccc cgacggattg accgttaccg tgggggtggg cagcagcctg tttgacggcc
      541 ggttcggact caaagacaaa aaaacggttc atttgcagga aatgcgcgac ttccccaacg
      601 ataagctgca aaaaagctgg tgcgacggcg atttgagcct gcaaatctgc gccttcaccc
      661 ccgaaacctg ccaagccgcc ctgcgcgaca tcatcaaaca caccgcccaa accgccgtca
      721 tccgctggag tatcgacggg tggcagccta aatccgaacc cggcgcgatg gcggcgcgca
      781 acctgttggg cttccgagac ggcacgggca accccaaggt ttccgatccc aaaaccgccg
      841 acgaggtttt atggacgggc gtggccgcca acagcctcga cgaaccggag tgggcgaaaa
      901 acggcagcta tcaggcaaat cttgccgacg gtttcatctt cgtgcaaaac ctcctcaacg
      961 gcgaaccgct ggaagaatgc atcagcccct tcggcggcgg ctatttcttc gtcttgcccg
     1021 gcgtgggaaa aggcggattc ttgggacaag ggctgccggg cgtataaacc tgcctgacgg
     1081 acaaatgccg tccgaacctt gccaaacggg ttcggacggg attttactgt ttggacggtc
     1141 aggctttttt gacgaattcg gattttaaat tcatcgcgct gccgtcgatt ttgcagccga
     1201 tgttgtgatc gccttcctgc aggcgtatgc ctttgacctt cgtaccttgt ttgatcgtca
     1261 tcgagctgcc ttttaccttg aggtctttga tgaggatgac ggtgtcgccg ttttgcagcg
     1321 ccgcgccgtt ggcatcgcgc acttgagccg caaggtcggc ggcggattcg gtttcattcc
     1381 attcgtgggc gcattcgggg cagatgtatt gtccgccgtc ttcataggtg tattcggagg
     1441 cgcattgcgg gcaagggggt aatgacatgg tttgccgtcc ttatcggatg tttgttttgg
     1501 gatgccgtct gaaacctgaa accggcttca gacggcatgg cttttattgt ttgtcttttt
     1561 ccggccttac ccagccttcg atgacggttt ggcgggcgcg ggcgagggcg agtttgttgt
     1621 cttcgatatt gcgggtaatc gcgctgcctg cgcctgtggt tactttgttg cccagtgtta
     1681 caggggcaac cagtacgcag ttcgaaccga tgcgcacttc gtcgccgatg acggttttgt
     1741 gtttgtgcac gccgtcgtag ttggcaataa tcgtgcccgc gccgaagttg gttttgctgc
     1801 cgacttcggc gtcgcctatg taggtgaggt ggttggcttt ggtgcctttg ccgatggcgg
     1861 cgtttttgat ttcgacgaag ttgccgacgt gtacattgtc agccagtttg gcttgcggac
     1921 gcaggcgggc gtacgggccg attcggttgt tttcgccgac ttcgcagcct tcgaggtggg
     1981 agaagggggc gattttgctg tttgcgccga ttttggcgtt tttgatgacg cagtttgcgc
     2041 cgatttcgac gttgtcgccg agttcgactt caccttcgat gacgacgttg acgtcaatca
     2101 cgacatcctg tccgtgtttc agacggcccc gcaaatcgaa acgcgccgga tcgcgcaggg
     2161 tcacgcctgc tttgagcaat tcttgcgcct gttcggtttg gaagatgcgt tcgagttcgg
     2221 cgagctggcg tttattgttc acgccggcgg cgaggtggga ggcgcgcact cggacggggc
     2281 ggactttgat gccgtcggca acggctttgg cgatgaggtc ggttaaataa tattcgcctt
     2341 gggcattgtt gctggaaagg ctgttcagcc agttttccag tttggcgttg ggcaggacga
     2401 ggatgcctgt gttgatttcg cggatggttt tttgggtggc acttgcgtct ttttcttcga
     2461 caatggcggt tacgctgccg ctgccgtcgc ggatgatgcg ccccaagcct gtcgggtcgg
     2521 cgggcacgtc ggtcaacagc ccgacttcgt tgcctgcggc ttcgagcagg gtttcgaggg
     2581 tttcaacgtc aattaaagga acgtcgccgt acaacaccag cgtgcggcct tcggaggcaa
     2641 ggtggggcag ggcggttttg acggcgtggc cggtgccgag ttgttcggtt tgttcgaccc
     2701 agacggcatc gcgtttgaca gtgtctaaaa cttgctcttt gccgtgtccg acgacgacgc
     2761 agatgttttg cggattcagc gcggcggcgg tgtcgataac gcgctcgacc atgggcttgc
     2821 cgccgatgca gtgcagcact ttgggcattt gggaatacat gcgcgtgcct ttgccggcgg
     2881 cgaggatgac ggtgtttaaa gtgttttgcg gcatagcgtt ttcctgtgcc atgccgtctg
     2941 aagcggtttc agacggcata gggtaggttt atcggttttg aaactttggt ttttgccagt
     3001 gttggcgatg ctcttcgtcg gcgttgttgc cggtttggtc gggcagcacg gcgcggcgtt
     3061 cgggacggta ttggttgtag ttcatatttt tcgagtagct gccgtcttgg taataaacgg
     3121 gcgtgccggc gggatatttt tgacggacgg cggttttgcc gttgccgtct tgataagttt
     3181 cccacgcgca gcccgacaaa agggcggcgg agacggtcag gataaggaag gttttacgca
     3241 tagcatt
//
LOCUS       DAWTHD010000147         3235 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWTHD010000147 DAWTHD010000000
VERSION     DAWTHD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.147"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(181..293)
                     /locus_tag="WM704_002013"
                     /pseudo
     CDS             complement(181..293)
                     /locus_tag="WM704_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(391..1431)
                     /locus_tag="WM704_002014"
     CDS             complement(391..1431)
                     /locus_tag="WM704_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFA9341356.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            1594..2634
                     /gene="mutY"
                     /locus_tag="WM704_002015"
     CDS             1594..2634
                     /gene="mutY"
                     /locus_tag="WM704_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFA9341357.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2797..3225
                     /locus_tag="WM704_002016"
     CDS             2797..3225
                     /locus_tag="WM704_002016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341358.1"
                     /translation="MNTNLNDKDKAMDTAIRFQKRMRIPKFFFLILGITVVLAFIQDV
                     ITGSNFLQITIMQIIFVIYFSFGGIIILFYIIIAGICPYCHEFQKMNYGTSVGIGSDS
                     FTFFKGISPVPKIDYCSKCGKPLSPEAVNKVMNDKTDEEM"
BASE COUNT          859 a          800 c          746 g          830 t
ORIGIN      
        1 atcggtaaaa tgccgtgatg agcggctgaa actaacaagt taaccgtcgt taaaaaataa
       61 cgctcagaga cctttgcaaa attccttttc ttcaaacagc cgacatccaa ccacaggttt
      121 ttcggctgtt ttcgtttcaa ataaccgccg ttcccaccaa aatatcccat taatccccct
      181 taaatatagt ggattaaatt taaaccagta cggcgttacc tcgccttgcc gtactatttt
      241 actgtctgcg gcttcgttgc cttgtcctga tttaaattta atccactata cctgattgat
      301 caggaacagc ccgttttaca cggctgaaaa aatgccgtct gaaacacgtt cagacggcat
      361 ccgcttaaaa aacaactgat tcaacggcga ttaatccgct tccaaaacca ctttcatcgc
      421 ttggttttcg gcggcgtgtt tgaacacgtc gtaggctttt tccaattcgc tgaatttgaa
      481 acggtgggtc aacattttgg tgtaatccac tgagctgctg aaaattgcct tcatcagtat
      541 ttcggtggtg ttggcattga ccaaaccggt agtgatggca agatttttaa tccacagttt
      601 ttccagtttg aaatcaacgg attgaccgtg tacgccgacg acggcgatat gaccgccggg
      661 tttcacgatg tcttggcaca tattccaagt tgcagggata ccgaccgctt cgatggcaca
      721 atctacgccg tcttcgccga cgatggcgaa aacttgtttg gagacgtcgc cggaagcggg
      781 gctgatggta tgggtcgcgc ccaactcttt cgccggtttc aaacggtttt cgtccatatc
      841 gcacacgata atggcggcag ggctatacag ttgggcggtc aacaaggcgg acataccgac
      901 agggccggca cctgcgatga atacggtatc gccgggtttc acatcgccgt attgcacgcc
      961 gatttcgtgg gcggtcggta aagcgtcgct caacagcagg gcgatttctt cgttgacgtt
     1021 gtcgtgcggc ggcacgaggc tgttgtcggc ataaggcgtg cggacgtatt cggcctgcgt
     1081 gccgtcaatc atatagccca aaatccagcc gccgttgcgg cagtgcgaat aaagttggat
     1141 tttgcagttg tcgcaagtgc agcatttgct gacgcatgaa ataatgactt tatcgccgac
     1201 tttgatgttt tttacagcct cgccgacttc ttctacaata ccgatgcctt catgaccgag
     1261 gatacgaccg tcggcgactt cggggttttt gcctttccaa atacccaagt cggtaccgca
     1321 aatcgtggtt ttgacgattt tcaccaccgc atcggtcgga tcgataatct gcgggcgggg
     1381 tttttcttca aaacggatgt cgtttgcgcc gtgataaacc attgctttca tgctgatact
     1441 ccttgcttgt tgataaataa tttcaatacc gcaacaaagt ttctttatat gagttatatg
     1501 cccctacaaa aaataagtca ataagaatta ttttcacaat tttatacaat aacatactgt
     1561 tttaaatata aataaaacca ccgattgata ttaatgaaca cacccatccc cttctccgaa
     1621 cggctcatcc gctggcaaaa acaacacggt cgccaccacc tcccttggca ggtcaaaaac
     1681 ccttattgcg tctggctttc cgaaatcatg ctccagcaaa cgcaggtcgc cgccgtgttg
     1741 gactactatc cgcgtttctt ggaaaaattc ccgaccgttc agacgcttgc cgccgcgccg
     1801 caagacgaag tgttgtcgtt gtgggcgggc ttgggctatt acggccgcgc gcgcaatctg
     1861 cacaaagccg cgcaacaaat cgtcggacaa ttcggcggta cgtttccatc ggagcgcaaa
     1921 gacttggaaa cgctctgcgg cgtaggcaga agcaccgccg ccgccatttc tgcctttgct
     1981 ttcaaccgac gagaaaccat cttggacggc aacgtcaaac gcgttctctg ccgtgttttt
     2041 gcccaagacg gcaatccgca agataaaaaa tttgaaaact cgctctggac acttgccgaa
     2101 agcctgatgc cgtctgaaaa cgccgatatg cctacttaca cgcaaggttt gatggatttg
     2161 ggcgcgaccg tgtgcaaacg gacgaaaccc ttgtgccgtc aatgccctat ggcggacatc
     2221 tgcgaagcaa aaaagcaaaa ccgcaccgcc gagctgccgc gtaaaaaaac tgccctcgaa
     2281 gtgcaaaccc tgccgcttta ctggctgatt gtccgcaacc gggacggcgc aatcttgctg
     2341 gaaaaacgca ccgccaaagg catttggggc ggactgtatt gcgtgccgtg ttttgaaagt
     2401 ttgaacgggc tttccgactt tgccgccaaa ctctccctga cgatggcgga tatggacgaa
     2461 caaaccgccc tgacccaccg cctgacgcac cggctgctga tgattacgcc gtttgaaggg
     2521 caaatgccgt ccgaacacca ttcagacggc atttggataa agccgggcca tttgaaagat
     2581 tacggcctgc ccaagccttt ggaaatttat ttaaacggta ataggttaga ataaacaaaa
     2641 taaacccgtt gaactgttgt tagcaggtat cgcagcaaga acaaccgatg aatttgggtc
     2701 gtattttagg cagcgggata atgttcaaat gggacatttg gaacggaaaa agtcggcaat
     2761 ttaaaaagga ttttagaaac aaagagggtc aaaaacatga acacaaactt aaatgacaaa
     2821 gacaaagcca tggataccgc aatcaggttt cagaaaagga tgaggattcc gaaatttttc
     2881 tttttaattc tcggaatcac agtggttttg gcatttatcc aagacgtgat aacgggttct
     2941 aattttctgc aaataacaat tatgcaaata atatttgtga tatatttcag tttcggaggt
     3001 atcataattt tattctacat aatcatcgca ggaatctgcc cctattgcca cgaattccaa
     3061 aagatgaatt atggcacctc cgtcggcatc gggtcggata gctttacttt ctttaaaggc
     3121 atttcccctg tcccaaaaat cgattactgc agcaaatgcg gtaagcccct gtctccagaa
     3181 gccgtaaata aggtgatgaa tgataaaacc gatgaagaaa tgtgaacagc gtttt
//
LOCUS       DAWTHD010000148         3094 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWTHD010000148 DAWTHD010000000
VERSION     DAWTHD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.148"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            180..689
                     /locus_tag="WM704_002017"
     CDS             180..689
                     /locus_tag="WM704_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFA9341359.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(919..1500)
                     /locus_tag="WM704_002018"
     CDS             complement(919..1500)
                     /locus_tag="WM704_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFA9341360.1"
                     /translation="MEHKLPQLPYGPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTFNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            1663..3066
                     /gene="dnaB"
                     /locus_tag="WM704_002019"
     CDS             1663..3066
                     /gene="dnaB"
                     /locus_tag="WM704_002019"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFA9341361.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSITKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKVILTWMGQ
                     FTKFGNAAYIPESAAED"
BASE COUNT          762 a          738 c          782 g          812 t
ORIGIN      
        1 attttgggac tgcaacaaac gcaagcattg atttgcgttt gttgcaaact tattattata
       61 gcaggttgcg gcgcggactt gatgggaatt gtttatagag catgatagtc tgaaacatat
      121 taattaaaaa taccattaat ccgatttata tttatttcaa tttcaatgga aaaacatcaa
      181 tgacaatgat tttaagcatt ttaagcctgt tttttatcat tagactgtta tttttagccg
      241 tctctattaa acatgaaaaa gccttgattg ccaaaggggc gaaacaatac ggaaaaacca
      301 attccacggt gcttgcggca gttcatacgc tttattattt ggcgtgtttt gtttgggtat
      361 ggctttctga cactgctttt aatggcatat ccttgattgg tacactgacg gtgatggctt
      421 catttgtgat attatcattg attattaagc agttggggga gatttggacg gttaaaatct
      481 atattttgcc aaatcatcaa attaatcgtt cgtggttgtt taaaacattc cgccacccga
      541 attatttttt aaacatcata cccgaactga ttggcatcgc cttattatgt caagcgtggt
      601 atgttttatt gattggcctg cccatttatt tgctggtctt atttaagcgt atccgacaag
      661 aagaacaggc gatggcaaca cttttttaac ccgtttcatc aattaaaaat atatcgacaa
      721 agagaaccgt ctatcaatta aatccattct tatcattggg gcaggcttat tgtcattggg
      781 caagccgtga caaaagcccg aacctgcaaa acttatctat aaggctgcat cagtgtgtat
      841 tgaaaccccg cctttttgac aacaaatgcc gtctgaagcc ttcagacggc atttgttcgg
      901 tttggcaaag ccggcggctt atgcggcaaa acgtttggcg acttcgtccc agttgacgat
      961 ttcccaaaaa ccttccaggt agttggggcg gctgttgcgg tagtcgatgt agtaggcgtg
     1021 ttcccacacg tcgcaagtca agagcggcgt gttttcagtg gtcagcggcg tagcggcgtt
     1081 gaaagtggaa atcaaatcca atccgccgac aggggttttc accagccacg cccaaccgga
     1141 gccgaacgta ccggccgcgc aggcattgaa cgcttcttgg aatttttcga agctgcccca
     1201 tttcgcgtcg atggcggcgg ccagttcgcc ggcaggcttg ccttggcctt tgggcgtgaa
     1261 acccagccag tagaaggtgt ggttccaagt ttgagccgcg ttgttgaaca cgccgcccga
     1321 agattttttc acaatctctt ccaaaggcag gttttcaaat tcggtgcctt tgatttgatt
     1381 gttcaggttg gtgatgtagg tttgatggtg tttgccgtag tggaattcca aagtctcttt
     1441 gctcagatgc ggggacaatg cgtccggccc ataaggcagt tgcggcagct tatgttccac
     1501 tttgtgctcc taatattgtt ttaaatgttg tattttggca gtgttgctgc aaacgaatcg
     1561 gcagctcacg cattctacct gttttgagtg atggaaacca attaaacctg ctttgcgtta
     1621 taatagaaga ttgcaatttc ggcacgacag attggatgta ccatgaacga ttacgcagct
     1681 atgccgcctg aaggccgtga ggtcggcgca ttgtcgctgc ctccgcattc gatggaggcg
     1741 gaacaatccg ttttgggcgg tttgatgctg gaaaatccgg cttgggacag gattgccgat
     1801 gtggtttcgg gagaggattt ctaccgccac gaacaccgcc tgattttccg atccattacc
     1861 aaattaatca atgagggccg tcccgcagat gtgattacgg ttcaggaaga tttgcagcgg
     1921 aacgaagaat tggaagcggc aggcggattt gaatatctga ttacactggc gcaaaacacc
     1981 ccgtctgccg caaacatccg ccgctacgcc gaaatcgtac gcgagcgttc cattatgcgc
     2041 caactcgccg aagtggggac ggaaatcgcc cgcagcgcat acaatccgca aggcagggac
     2101 gcggggcggc ttttggatga ggcggaaaac aaagtgtttc aaatcgccga aagcaccgcc
     2161 aaatccaagc agggcttttt ggagatgccc gatttgctga aagaagtcgt acagcgcatc
     2221 gatatgcttt attcgcgcga caatcccgat gaagttaccg gcgtaccgac agggttcatc
     2281 gaccttgata aaaaaacctc gggtctgcag cccggcgacc tgattatcgt cgccggccgc
     2341 ccgtctatgg gtaaaaccgc gttctccatc aatattgccg agtatgtcgc cattgaaaaa
     2401 catttgcccg tcgccgtttt ctcgatggaa atgggcggtg cgcagttggt catgcgtatg
     2461 ctcggctcgg tcggacggct ggatcaaagc gttttgaaaa ccggcaggct ggaagacgaa
     2521 cattgggggc gtttgaacga ggcagtcgtc aaactttccg acgcgcctat gtttattgac
     2581 gaaactccga atctgaccgc cctcgaattg cgcgcccgcg cccgccgcct cgcccgccgg
     2641 tttgacggca gattaggctt gatcgtcatt gactacctgc aactgatggc ggcatccggc
     2701 cgttcggaca accgcgcttc cgagcttggc gaaatttccc gttccctcaa agccctggca
     2761 aaagagttgc aagtccccat catcgccctg tcgcaattga gccgcactgt cgaacagcgc
     2821 accgacaaac gcccgatgat gtccgacctc cgcgaatccg gtgctatcga gcaggatgcc
     2881 gacctgatta tgtttatgta ccgcgacgaa tactacaacc aggactcacc catgaaaggc
     2941 cttgccgaat gtatcatcgg caaacaccgc aacggcccgg tcggaaaagt catcctgacg
     3001 tggatggggc agtttaccaa attcggaaat gccgcctata tcccggaatc ggcagcggaa
     3061 gactgaatgg catagtatag tggattaaca aaaa
//
LOCUS       DAWTHD010000149         3048 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWTHD010000149 DAWTHD010000000
VERSION     DAWTHD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.149"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..209
                     /locus_tag="WM704_002020"
     CDS             <1..209
                     /locus_tag="WM704_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341362.1"
                     /translation="ILTKARRMYSWWAKESISLSGVARHGIRQDGSQICGELDSVWLE
                     AIEIIPVTGGAAESIRTALEVAQS"
     gene            206..493
                     /locus_tag="WM704_002021"
     CDS             206..493
                     /locus_tag="WM704_002021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341363.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGDGYGNGYGNGYGSGYGNGKGGGS
                     GSGYGNGKGDGDGSGNGDGDGNGNGSGNGSGNGDGDGNGNGSGNGSGYGNG"
     gene            634..966
                     /locus_tag="WM704_002022"
     CDS             634..966
                     /locus_tag="WM704_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341364.1"
                     /translation="MKYYGTAAYGSPDWGMERYYAREDMRQALDGWEAENRILHESAL
                     IGIAKKSAREFVRDADGEPYSQEDWETYLTEDASRIGKDTEAAMNYAIDEREWFALAE
                     NIGRLANS"
     gene            1119..1397
                     /locus_tag="WM704_002023"
     CDS             1119..1397
                     /locus_tag="WM704_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341365.1"
                     /translation="MKTRNIAFKFAVLAAVLAAGYAFGFAKGGGSRTAKGKPAGIAAM
                     RMALAQKQAEVAELSAEIWLEERHLNADEEEAGCRRVHGDAEVPGGKE"
     gene            1394..1555
                     /locus_tag="WM704_002024"
     CDS             1394..1555
                     /locus_tag="WM704_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341366.1"
                     /translation="MSFHPETAYTGGGETEPYGPSPEEIKYRQSPETAETRRMTEKQA
                     EGHIKSIIR"
     gene            1624..2310
                     /locus_tag="WM704_002025"
     CDS             1624..2310
                     /locus_tag="WM704_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341367.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDNAVFAFDLS
                     DPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQAAEGRTEHPQGNAAPA
                     EDIEDDIPFN"
     gene            2450..2632
                     /locus_tag="WM704_002026"
     CDS             2450..2632
                     /locus_tag="WM704_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341368.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            2629..>3048
                     /locus_tag="WM704_002027"
     CDS             2629..>3048
                     /locus_tag="WM704_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFA9341369.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEEHIGRMTGKLKAAKARNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIR"
BASE COUNT          861 a          827 c          910 g          450 t
ORIGIN      
        1 tgattctgac aaaagcgcgc cgaatgtaca gctggtgggc aaaggaatca atcagcctgt
       61 caggtgtcgc acgacacggc atcaggcaag acggcagcca aatttgcggc gagcttgatt
      121 ccgtatggct cgaggcgatt gagattatcc cagtaacagg cggcgcggct gaatcaatcc
      181 gcaccgcgct ggaggtcgcc cagtcatgag ttatctagat caaccattga agcacggcta
      241 cggcaacggc aacggtagcg gcaacggcga cggctatggc aacggctacg gcaacggcta
      301 cggcagcggc tacggcaacg gcaagggcgg cggtagcggc agcggctacg gcaacggcaa
      361 gggcgacggc gacggcagcg gcaacggcga cggcgacggc aacggcaacg gtagcggcaa
      421 cggtagcggc aacggcgacg gcgacggcaa cggcaacggt agcggcaacg gtagcggcta
      481 tggcaacggc taaaccttaa aaccgcccga agctatatct gccaagccgg ggatagcaca
      541 aagcgatgaa gtatcgaact tcaaagcggg gcaacggcac ggcgaaacag tgaatgctgc
      601 gggcggtttt ctcaaacgga aaggacgaag gcggtgaaat attacggcac ggcggcttac
      661 ggcagccccg attgggggat ggaaagatat tacgcgcggg aggatatgcg gcaggctttg
      721 gacggttggg aggcggaaaa ccgaatcctg cacgaatccg cgctaatcgg aatcgccaaa
      781 aaatcggcgc gggagtttgt ccgcgatgcg gacggcgaac cttacagcca ggaggattgg
      841 gaaacgtacc ttacggaaga tgcttcccgt atcggtaaag ataccgaagc cgcgatgaac
      901 tacgccatag acgagcggga atggttcgcg ctggcggaga acatcggcag gctggcaaat
      961 tcatagcggg cataggaatg ccgcgccgtc agtcggcgag tgggcgcggc ggcggttttt
     1021 gtagatttta gccgcaaata accccgacgg cgcacggtcc gtaccatcct tagcggacgc
     1081 ggtgcgcaat taaaaccttt taaattaagg aaatagaaat gaagacaagg aatattgcgt
     1141 ttaagttcgc ggtattggcg gcggtattgg cggcgggcta tgccttcgga ttcgccaagg
     1201 gaggcggaag ccgtacggca aagggaaaac ccgccggtat tgcggcaatg cgcatggcgt
     1261 tggctcaaaa gcaggcggaa gttgcggaat tgagcgccga gatctggctg gaagagcggc
     1321 acctgaatgc cgatgaagaa gaagccggtt gccggcgggt gcacggcgat gcggaagtgc
     1381 cggggggtaa agaatgagct tccatcccga aaccgcttac accggcggcg gagaaacaga
     1441 gccgtacgga ccaagccccg aagaaatcaa ataccggcaa agcccggaaa ccgccgaaac
     1501 acggcggatg accgaaaaac aggcagaagg ccacattaaa agcattatca ggtaacgccg
     1561 ccgcgcaagg cataaaacag cgcgagaccg atagacgaaa cgacctttaa caggagaccg
     1621 aaaatgtcat tgattttaag tgtgaaagac gaaagcaatt tcaaaccatg cccggcaggc
     1681 agccatcacg ccacctgcat ccgcatcatc gatttgggta cgcagcttgt cgagtaccaa
     1741 aacgaacaaa agcggcagca caagatttta gtgcagtggg aaatcgaccc ggaaggcgat
     1801 ccggaaatgc tgatgccgga cggcaggccc tacctcatca gccgccggta caccgccagc
     1861 ctgcacagca aaagccaact ggcaacagac ctcaaaagct ggcgcggaag ggactttaca
     1921 ccggaagaac gcgacaactt cgatttgcgc aatattttgg gcaagccctg cctgttgagt
     1981 atcgcccacc aagaaagcag cgacggcaaa accacctatg ccaacatttc cgccatcagc
     2041 aacaagatga agagctacac cccgaaacag ccggacaacg ccgtttttgc cttcgacctg
     2101 tcagaccccg attgggcgaa ttacggcctc ctgaacgaga aactgaggga gcagattgcc
     2161 aaaagcccgg aatatgccga agccgtaaac ggccgccaac cgccggcgcc gccgcagaaa
     2221 caggcccaag cggcggaagg gcggacggaa cacccccaag gcaatgccgc gcctgccgaa
     2281 gacatcgagg acgacatccc ttttaattaa gcccgccgtc agggcgtaaa accggacgga
     2341 aaaccgcaaa caaacgccgc gccctacggg caggccccgc actctgaagc agtagtcgat
     2401 ttttagtttg caactgcttc ggacggcaac agagaaagga aacaaaaaaa tggcaaacat
     2461 cgacctgacc caatgggacg ggaaaaccat tggcgccgcc gccaatcccg aacagggcta
     2521 catcaacatc accatcggca gcgacgacct attcatcaac atcgaacagg catacgccat
     2581 acacgccgcg cttggcgaag cggttgccga atatgaggga ggggcacaat gaccgccctc
     2641 acactctacc ggtgcgcggc agacgtacag gcggcgcttg attactactt cgacagcgaa
     2701 accgagcgcg aagacacgct ggaagccgtt atcgggcagt tcgaggtcaa agcgcaatcc
     2761 gttatcgctt atattaaaaa ccaagaaatc acggaaaaaa tgcttgaaga gcatatcggg
     2821 cggatgaccg ggaagctcaa ggcggcaaaa gcgcggaatc aaagcctgaa agactacttg
     2881 gcgcgcaata tgcaggcggc gggcattacc gaaatcaaag cggacgacgg cacttttaaa
     2941 gcctcgttcc gcaaatccga agccgtcgtg atcttagacg aagcacaaat ccccgccgaa
     3001 tttatgcgtg aggccgtcaa aaccgaaccg gacaaaaccg ccatcaga
//
LOCUS       DAWTHD010000150         2985 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWTHD010000150 DAWTHD010000000
VERSION     DAWTHD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.150"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..173
                     /locus_tag="WM704_002028"
     CDS             <1..173
                     /locus_tag="WM704_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFA9341370.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1036
                     /gene="cas8c"
                     /locus_tag="WM704_002029"
     CDS             170..1036
                     /gene="cas8c"
                     /locus_tag="WM704_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFA9341371.1"
                     /translation="MILASLVRYYRRLATETDETGNPKVPSYGFSEEKIGWILVLDKE
                     GRLKTVVPNLTADKKPQPKLMSVPRPEKRTSGIKPNFLWDKTAYALGVEANKNKAEAK
                     EKPFTPSEKTFEAFKQYHLDLLQNSEDEGLQALCRFLQNWQPAHFAAENLPAEMLDSN
                     TAFSLEKPTALIHKREAAQTLWAGCLKSDEALESLCLISGDTAPIARLHPAIKGVFGG
                     QSSGGSIISFNKEAFSSFGKEQGANAPVSEQSAFAYTTALNYLLRRENNHCLTIGDAS
                     TVFWAEADDIVD"
     gene            1024..1128
                     /locus_tag="WM704_002030"
                     /pseudo
     CDS             1024..1128
                     /locus_tag="WM704_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1121..1528
                     /locus_tag="WM704_002031"
     CDS             1121..1528
                     /locus_tag="WM704_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFA9341372.1"
                     /translation="MHYNATAQAAEGFFAQVFTPSDDEQESAKIFNVLEQIGKGRPLQ
                     EIAPELSPNTRFYILGLAPNAARISVRFWLDTTFGQLAENLAHHWQDLALEPCAWKTP
                     PSIWRLLLQTAVLGKSENIQRLGNRCLPETQNP"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="WM704_002032"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="WM704_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="WM704_002033"
     CDS             2263..2631
                     /locus_tag="WM704_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341373.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..>2985
                     /locus_tag="WM704_002034"
     CDS             2638..>2985
                     /locus_tag="WM704_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFA9341374.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNAQAWAE
                     NCLTAQGKALHERVDSDEPETHKGVRFERTVHVSAEKPGISGILDLVEADTKTGRLKP
                     VEYKRGKPKPDPGD"
BASE COUNT          836 a          719 c          767 g          663 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcggcga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aagcactcgg cattgagtct gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 cattaccgcg tggatgtgta aaaacttctt cgatatccga accttcggcg ctgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacacgcatg gcggtaacca acgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgtgg tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctcagccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacaccg taaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgcgcaggcg
     2761 tgggcggaga actgtttgac cgcgcagggc aaggcactgc atgagcgggt ggattcggac
     2821 gagcccgaaa cgcacaaggg cgtgcgtttt gagcggacgg tacacgtttc ggcggaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata caaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggat
//
LOCUS       DAWTHD010000151         2956 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWTHD010000151 DAWTHD010000000
VERSION     DAWTHD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.151"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            30..1920
                     /locus_tag="WM704_002035"
                     /pseudo
     CDS             30..1920
                     /locus_tag="WM704_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745759.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1977..2954
                     /locus_tag="WM704_002036"
     CDS             1977..2954
                     /locus_tag="WM704_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFA9341375.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
BASE COUNT          831 a          860 c          702 g          563 t
ORIGIN      
        1 tgaaactaaa tactctcaca tgggctttga tgaccgtttt ttccgttgcg ccatcttggg
       61 cagaacaacc ggcaaatact gaagaaatac aacccgtcaa aaccttctcc ccgcccaaac
      121 cgattgcacc gaccgccgca caaggctatt tccccgaaaa ccaattcgac cgctccgacc
      181 gcagcgatta ttactttgtt accgaaaaca tagaccaagc cttccgtccg ctgaaggcaa
      241 acagcagttt ttacggcaaa agcttttaca actccgttac cgcgcaagca ctcggggcga
      301 aggtatacgg cgtagccaac ctcaatcgca ccaaggcaaa cggctacaaa gatggcggtg
      361 ggcgcgacac tgattggaaa tacagccgct tcaatcaggc tttggtactc ggtttcgtgc
      421 cgtctgaaaa tcaagaatac cgcctcactt atctgcacga cgacatcaac aacgaccgcc
      481 agccgcaggt cgtcaacgac gcattggaca ccgaacgcca catctccaaa ctcaacgtgc
      541 gttggggcaa tgccgatttg agcaatacgg tcagcgcgga agcaggcgtc atcaaactca
      601 aacgccatgc cgacaattac tccttgcgac caaacaacac gccgcagcaa gtgttcgtag
      661 aacttgaccg caaagtgtat gatttctcgc tcaaacacga tgcggatttc ggcaaattcc
      721 acaacacggc agccgtcagc taccgcaacg acagccaaaa cggcgaacgc aacacccaca
      781 ctgccatgtg cgatttcctc aacggctacc gtttcgccga tgtgcacatc gaccgctggt
      841 gcattgccga taccttatct tacaagtttg acgaccgaca caaactgggc ttgggcttaa
      901 gctacgaact caacgaagcg gacattcgca aaaatacggc acagcctgaa aacccaataa
      961 aacccggttt ccccttcgcc tcatcacaac aaatctggaa aacccattac ggctacgact
     1021 tcaacggcaa agtgcgccgt catgctttgt caggcgaact caaatacgat ttcacgccgt
     1081 ctgaaacgca aaaatacagc gtttccctcg cgcatttgga acgcatcggc gacaacaccg
     1141 aacgcttcaa ctcgcttgct gccatcgtgc aaaaccgtat gagcggcatg ctgatgaacc
     1201 aaaatccagc cgccgccatt gcaggcaatc cccctgctga aaactgaaaa acacaaccgc
     1261 atcaggctca ccgccgacag ccgcaacgac tactacaacg gctacatgaa ctcgctcgca
     1321 ggcgcgggct ggaacgtggg cggcacgctt gtggcggaca aagtcaaaga cctgattatt
     1381 tttgaccgcg cacacggaca aagcggcaca gcttccaaag acggcggcat catcacacgc
     1441 aacgtggacg cacgattgtt taccgcgcag gcctacgcgc gttacaactt caatccgcat
     1501 tgggcggcag gcatcaaagc cgcctacaac tacggacaca acgaaaccga cggcaggccg
     1561 ccctatcaaa tccgtccgtt tgaagccgcc gtccaagccg actacaaaaa ctactttgcc
     1621 cacggcagct acaacatcgg cgcggcaaca cgctttgtcg ccaaacaaac gcgcggtgat
     1681 tttgatatgg caagcggtct gggcatagac aaacgcgaag ccgccaaagg ctttaccgtt
     1741 gccgacgtgt acgcaggcgt aaacatcaaa gacaaatacg gcttgcgctt gggcgtgaac
     1801 aacgtattca acaaaaaata cgtcgaatac atcagcggcg accacgtcct cgccctatcc
     1861 cccagcgtag tgtatgcacc gggcaggaca tattggttga gtttgcatgc ggcattttaa
     1921 aaccatgccg tctgaaaaat acctgcattg gcttattaag aattaggaga taaaaaatgg
     1981 atatgaaaag acgcgatttc ttaaaaatga ccgccgcgct ggcagccgca ggcgtttcgc
     2041 cttctctgct tactgccggt aaagagcaat ttaccgtgta cggcgcaccg gcaatgccca
     2101 gcgttaccat tgccgtaacg gcgttgcaag gtaaactggc gaaacaggcg gacgtgtcgc
     2161 tgaaaatctg gcgttcgccc gaccaactgc gcgcgggcgt ggcaagcggg caatttaaag
     2221 tgatgatgag tccgagcaat gtcggcgtaa acctgcgcaa ccaagggcag aaagtcggca
     2281 tggtgaatat tttgaccaac ggcatcacgc agttggtctg caaaggcagc gcgattgcct
     2341 cgccgcagga tttggtcggc aaaaaaatcc tcgtgccgtt taaaaacgat atgcccgaca
     2401 tcgtgctgca agccttgttg aaaaaactga aaatcgacgc acacaaagtc agcattacct
     2461 acgctgccac accgcccgaa gcagtcggac tatttccaag taaaggttac catgccgtca
     2521 tcctgcccga accgatggca accgccagcc tactgaaagg caaaaccata ggtataaacg
     2581 tcgtgcacgg ttttgatttg gtaaaagcat gggggcaggc gtttggcacc aaaccgctga
     2641 ttccgatggc aggtatcatc gccaacgaag aatatttcca cgcacacaag gcgcagttcg
     2701 acatcttcca tcaagatttg aaaaacgcgc tcaactggat attggccaac cgccaaaacg
     2761 cggcgaaaat cggcaaaaac tacctacccg cccccgaacc cgccctagtc atgggcttgg
     2821 acggcgcacg gctgacggta agcaaaggca gcgaagtgaa aaacgagatt ttgaagtttt
     2881 acgaaatcct gatgcagttc aacccggaac ttttgggcgg caagctgccg gataacgggt
     2941 tcttcttggc ttaaag
//
LOCUS       DAWTHD010000152         2944 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWTHD010000152 DAWTHD010000000
VERSION     DAWTHD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.152"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(124..576)
                     /locus_tag="WM704_002037"
     CDS             complement(124..576)
                     /locus_tag="WM704_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9341376.1"
                     /translation="MSDFQIIFEYCRCSKVRIPARAQVSEAILLAEGQKSAVTEYYLN
                     HGIWPKDNASAGVASTPTDIKGKYVQKVEVNNGVVTAQMNPSGVNKEIQGKRLSLWAK
                     RQDGSVKWFCGQPVTRDNAGKADVAADATAGIDTKHLPSTCRDNFDAS"
     gene            complement(762..1517)
                     /locus_tag="WM704_002038"
     CDS             complement(762..1517)
                     /locus_tag="WM704_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFA9341377.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(1695..2873)
                     /gene="nirK"
                     /locus_tag="WM704_002039"
     CDS             complement(1695..2873)
                     /gene="nirK"
                     /locus_tag="WM704_002039"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HFA9341378.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
BASE COUNT          607 a          719 c          876 g          742 t
ORIGIN      
        1 accggaacgg acgaccccgc ccgccttgca aacccttaaa agacaagccg cccgggttga
       61 tccgggcggc cgtgggaaat cacttaccgt ttgatttatt tgaaatttaa ggcctaattt
      121 gcctcagctg gcatcaaagt tatcgcggca ggttgacggc aggtgcttgg tgtcgatgcc
      181 ggcggtggcg tcggcggcaa cgtcggcttt gccggcgttg tcgcgcgtaa ccggctgtcc
      241 gcagaaccat tttaccgaac cgtcttgacg cttggcccac agggagagtc ttttgccttg
      301 gatttctttg tttacgccgc ttggattcat ttgggcggtg acgacgccgt tgttgacttc
      361 aactttctga acatatttgc ctttgatgtc ggtgggggtg gatgccacgc cggcagaagc
      421 gttgtctttc ggccatatgc cgtgattcag gtaatactcg gtaacggctg atttttgacc
      481 ttcggccaaa aggatggctt cggaaacttg cgcgcgggcg ggaatccgga ccttggaaca
      541 acggcaatat tcaaagatta tctgaaagtc cgagattcca gattcccgct ttcgcgggaa
      601 tgacgaaaag ttgcgggaat gacggttcgg gcattcctta aattacccgt gtatcgctgt
      661 aaatcttaga gatggcggaa tatagcggat taacaaaaac cagtacggca aggcgaggca
      721 acgccgtact ggtttttgtt aatccactat aattgaaggg gttatcggct tgcgcagcgg
      781 aagcccaagt tgtgcaagac atatttggat tgcaggctgg tgcggatgcc gtagcggagg
      841 aaggcggcat agttggacga gtcgctcgcc ccgacagatg cgccgctgca aaacatttgc
      901 gcgttggcat tgccggaaga aagcaggctg ctgttgaaat cttccgtcca ttcccaaatc
      961 agcccgtgca tatcgtaaac accccagtag ttcgggcggc ctttgccgac atcgtgcagg
     1021 cctttccgtc cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc gggttcgttt
     1081 gagccgtttt tctgcgtggc ggaagcaagt ccggcaaatt cccattcgtc gatggtcggc
     1141 aggcgtttgc cttgtgcggc gcaataggcg ttggcggcaa accaggaaac attggttacc
     1201 ggttgtttta attcgcccgc cttcggcgca tagctgcggc tgccgttttt catccaatgc
     1261 ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca ttgggggtgg
     1321 ctgttgacaa attcggcaaa ctcggcattg gtaacgggat atttatccag tttgaacggt
     1381 ttgactttaa tcaggccggt atcttttttc agataaagcg ggcggtagct gccgccttcg
     1441 atttgaacca tttcggcagc cgccgcttga gtgccggcga gtgccgtgcc gaggaaaaat
     1501 aaccggacgt acttcataaa gcctcctgac aggcggttag cattgatttt tcagacggca
     1561 ttttaaacaa aaagccgcga atgccgtctg aaaaatcaat cttccgaaag gaaagattgg
     1621 ttgttaaaaa accactgccg tgcgtaatga agtacggcgg cagtggtttg tcccgctaat
     1681 gacgggtatc cgatttaata aacgcttttt tcggatgcag aggctgccgg ggcagaagca
     1741 gccggtgcgg aagcagcagg ggcagaagcc gcgccgctgc cggcgtaagc ggtatcactc
     1801 aatttttgag tcatgatttc agggttttcc gcaccctcta ctttcaattg ccccaacgcg
     1861 cctttgttga atgcgcggaa gatggagtgg tcgaccaaag tgtagctgcc cgggatgtcg
     1921 actttgaatt cgacgatggc agaaccgccg gcaggcacga tggtgctttg tacgttttcg
     1981 ttaatcagtt tgccgccttc aacataaact ttgtcgaaga tttcgccgat gacgtggaag
     2041 gaagacacca agttcgggcc gccgttaccg acgtacatac gcacggtttc gcctgctttg
     2101 gctttcaggg cgttatcgcc ggcgatagcg cctacgtggc cgttgaatac gacgtattca
     2161 ggctgttcgg caacggcttt gtccatatcg aacggttgca ggccttgcgc gccttttttg
     2221 cctttggtgt agaagtcgcc ttggacgatg tagaactctt tatccacttt cggcaggcct
     2281 tctttaggct cgaccaaaat cagaccgtac ataccgttgg cgatgtgcat accgaccggc
     2341 gcgacggcgc agtggtagat gtacaggccc ggttgcaggg ctttgaagct gaatgtggaa
     2401 gtgcggcccg gggcggtaaa ggtcgcggct gcaccgccgc cctgaccggt tgcggcgtgg
     2461 aagtcgacgt tgtgcggaac ggtagaagaa ggattgttgg agaattcgac ttcaaccgta
     2521 tcgccttcgc gtacgcggat catacggccc ggaacgtcgc cgtcaaatgt ccagtagcgg
     2581 tattccaccc cgtcgtccat tttcatggtt ttttcgacgg tttccatttt tacgcgtact
     2641 ttggcaggat agtcgcggtc gattgcggga ggtacttcgg gagcgtgggt ggtcaccgca
     2701 tcgatgacgg gcagttcgcc tgcaggcgtt tcggcggtag cttgtgcggc ggaacttgcg
     2761 gcttctgcgg aagcggcagg ggtttcggca ggggcttgcg cggcaggttc gccaccgcag
     2821 gcggccaatg cgaataagga agcaatcatt gcggctaatg cttggcgttt cataatgttt
     2881 tccttttgta agaaaagtag gggggattag ttaagaactt ggtgtgatta tgcggttgtc
     2941 ccat
//
LOCUS       DAWTHD010000153         2784 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWTHD010000153 DAWTHD010000000
VERSION     DAWTHD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.153"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..718
                     /locus_tag="WM704_002040"
     CDS             <1..718
                     /locus_tag="WM704_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9341379.1"
                     /translation="VGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHKHGEIWKKPTR
                     HEDIAAVFPFGNPIHNNTMIMRRSVIDGGLRYDTGRDWAEDYQFWYDVSKLGRLAYYP
                     EALVKYRLHANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAA
                     YELPEKDLPEEDFERARRFLYRCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILY
                     RLIKNRRQARSDSAGKEQEI"
     gene            718..1557
                     /locus_tag="WM704_002041"
     CDS             718..1557
                     /locus_tag="WM704_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFA9341380.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWEQALDEGLPYIAVFEDDVLLGEGAEQFLA
                     EDTWLQERFDPDSAFVVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIISR
                     KAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRRLNRKQQWRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIV
                     PFQ"
     gene            1569..2489
                     /locus_tag="WM704_002042"
     CDS             1569..2489
                     /locus_tag="WM704_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFA9341381.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAAVAANLRGGGNIRFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     TDVLVRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     GFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEE
                     WRGKLAVPPTKHMLQRWRKKLSARFLRKIY"
     gene            2549..>2784
                     /locus_tag="WM704_002043"
     CDS             2549..>2784
                     /locus_tag="WM704_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9341382.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDILIVDDGSTD
                     GTPAIARHFQEQDGRIRIISNPRNLGFIASLNIGL"
BASE COUNT          674 a          736 c          782 g          592 t
ORIGIN      
        1 cgtgggcgag atggaaaaag accgcagcat catcgcgatg ggcgcgtggc tggaagtttt
       61 gtcggaagaa aaggacggca accggctggc gcggcaccac aaacacggcg aaatttggaa
      121 aaagccgacc cggcatgaag acatcgccgc cgttttccct ttcggcaacc ccatacacaa
      181 caacacgatg attatgcggc gcagcgtcat tgacggcggt ttgcgttacg acaccgggcg
      241 ggattgggcg gaagattacc aattttggta cgatgtcagc aaattgggca ggctggctta
      301 ttatcccgaa gccttggtca aataccgcct tcacgccaat caggtttcat ccaaacacag
      361 cgtccgccaa cacgaaatcg cgcaaggcat ccaaaaaacc gccagaaacg attttttgca
      421 gtctatgggt tttaaaaccc ggttcgacag cctagaatac cgccaaacaa aagcagcggc
      481 gtacgaattg ccggagaagg atttgccgga agaagatttt gaacgcgccc gccggttttt
      541 gtaccgatgc ttcaaacgga cggacacgcc gccctccggc gcgtggctgg atttcgcggc
      601 agacggcagg atgaggcggc tgtttacctt gaggcaatac ttcggcattt tgtaccggct
      661 gattaaaaac cgccggcagg cgcggtcgga ttcggcaggg aaagaacagg agatttaatg
      721 caaaaccacg ttatcagctt ggcttccgcc gcagagcgca gggcgcacat tgccgatacc
      781 ttcggcagtc gcggcatccc gttccagttt ttcgacgcac tgatgccgtc tgaaaggctg
      841 gaacgggcga tggcggaact cgtccccggc ttgtcggcgc acccttattt gagcggagtg
      901 gaaaaagcct gctttatgag ccacgccgta ttgtgggaac aggcgttgga cgaaggctta
      961 ccgtatatcg ccgtatttga agatgatgtc ttactcggcg aaggcgcgga gcagttcctt
     1021 gccgaagata cttggctgca agaacgcttt gaccccgatt ccgcctttgt cgtccgcttg
     1081 gaaacgatgt ttatgcacgt cctgacctcg ccctccggcg tggcggacta cggcgggcgc
     1141 gcctttccgc ttttggaaag cgaacactgc gggacggcgg gctatattat ttcccgaaag
     1201 gcgatgcgtt ttttcttgga caggtttgcc gttttgccgc ccgaacgcct gcaccctgtc
     1261 gatttgatga tgttcggcaa ccctgacgac agggaaggaa tgccggtttg ccagctcaat
     1321 cccgccttgt gcgcccaaga gctgcattat gccaagtttc acgaccaaaa cagcgcattg
     1381 ggcagcctga tcgaacacga ccgccgcctg aaccgcaaac agcaatggcg cgattccccc
     1441 gccaacacat tcaaacaccg cctgatccgc gccttgacca aaatcggcag ggaaagggaa
     1501 aaacgccgga aaaggcgcga acagacaatc ggcaagatta ttgtgccttt ccaataaaag
     1561 gagaaaagat ggacatcgta tttgcggcag acgacaacta tgccgcctac ctttgcgttg
     1621 cggcaaaaag cgtggaagcg gcccatcccg atacggaaat caggttccac gtcctcgatg
     1681 ccggcatcag tgaggaaaac cgggcggcgg ttgccgccaa tttgcggggg gggggtaata
     1741 tccgctttat agacgtaaac cccgaagatt tcgccggctt ccccttaaac atcaggcaca
     1801 tttccattac gacttatgcc cgcctgaaat tgggcgaata cattgccgat tgcgacaaag
     1861 tcctgtatct ggatacggac gtattggtca gggacggcct gaagccctta tgggataccg
     1921 atttgggcgg taactgggtc ggcgcgtgca tcgatttgtt tgtcgaaagg caggaaggat
     1981 acaaacaaaa aatcggtatg gcggacggag aatattattt caatgccggc gtattgctga
     2041 tcaacctgaa aaagtggcgg cggcacgata ttttcaaaat gtcctgcgaa tgggtggaac
     2101 aatacaagga cgtgatgcaa tatcaggatc aggacatttt gaacgggctg tttaaaggcg
     2161 gggtgtgtta tgcgaacagc cgtttcaact ttatgccgac caattatgcc tttatggcga
     2221 acgggtttgc gtcccgccat accgacccgc tttacctcga ccgtaccaat acggcgatgc
     2281 ccgtcgccgt cagccattat tgcggctcgg caaagccgtg gcacagggac tgcaccgttt
     2341 ggggtgcgga acgtttcaca gagttggccg gcagcctgac gaccgttccc gaagaatggc
     2401 gcggcaaact tgccgtcccg ccgacaaagc atatgcttca aagatggcgc aaaaagctgt
     2461 ctgccagatt cttacgcaag atttattgac ggggcaggcc gtctgaagcc ttcagacggc
     2521 atcggacgta tcggaaagga gaaacggatt gcagccttta gtcagcgtat tgatttgcgc
     2581 ctacaacgta gaaaaatatt ttgcccaatc gctgtccgcc gtagtggggc agacttggcg
     2641 caacttggat attttgattg tcgatgacgg ctcgacggac ggcacgcccg ccattgcccg
     2701 gcatttccaa gaacaggacg gcaggatcag gataatttcc aatccccgca atttgggctt
     2761 tatcgcctct ttaaacatcg ggct
//
LOCUS       DAWTHD010000154         2679 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWTHD010000154 DAWTHD010000000
VERSION     DAWTHD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.154"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            68..205
                     /locus_tag="WM704_002044"
     CDS             68..205
                     /locus_tag="WM704_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341383.1"
                     /translation="MSREQRIRGARLPVSAYMQAEGAGKAREALDGRVEKVKGVDDEA
                     V"
     gene            189..1253
                     /locus_tag="WM704_002045"
     CDS             189..1253
                     /locus_tag="WM704_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341384.1"
                     /translation="MMKPSESLRAAGRPIAYYPKLAKPLGGVNAAILFGHFFYWNDKT
                     QYESGIYRTAEEIEIETGLSVQEQRTARAKLRERGVLIETEKRIEHRIYYKLNLDAFD
                     DLMLQHSGGGESTAPKCNINSPELQNQHSGGGESTAPKCNINSPELQNQHSGSEESTA
                     VIRTEDLTEDLAVYTPLPPNAGNGKGGLNADAFVSADAETCGRETGEPTSPKAESDSN
                     GNGGLSGKPKNANVPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRW
                     CEMLGTAAPNGKVRFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGN
                     FVKILEWHPPKTNQAARGRA"
     gene            1250..2611
                     /locus_tag="WM704_002046"
     CDS             1250..2611
                     /locus_tag="WM704_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFA9341385.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
BASE COUNT          738 a          630 c          806 g          505 t
ORIGIN      
        1 aatacaaggt cggtaactgt aaaaatcccg ttgcccatta ctcaatcgca aagtcaggaa
       61 ttgaaccatg agccgggaac aaagaatacg cggagcccgc ttgccggtgt cggcttatat
      121 gcaggcggaa ggcgcgggca aggcacggga agcgttggac gggcgggtcg aaaaagtgaa
      181 aggggttgat gatgaagccg tctgaaagtt tgagagcggc aggcaggcca atcgcgtatt
      241 acccgaaatt ggcaaagcct ttgggcggtg taaatgcggc aatattattc gggcatttct
      301 tctactggaa cgataagacg cagtatgaat caggcattta ccgaacagcg gaagaaattg
      361 aaattgaaac cgggctgtcc gttcaggaac aaagaacggc acgggcaaag ctgagggaac
      421 gcggcgtatt gattgagact gaaaaacgaa ttgaacaccg catttactac aaactgaatt
      481 tagacgcttt tgatgatttg atgttgcaac attcgggggg tggggaatca acagccccga
      541 aatgcaatat caacagcccc gaacttcaaa atcaacattc ggggggtggg gaatcaacag
      601 ccccgaaatg caatatcaac agccccgaac ttcaaaatca acattcgggg agtgaggaat
      661 caacagccgt tataagaaca gaagatttaa cagaagattt agcagtatat acccccttac
      721 ccccaaacgc cggaaacggc aaaggcggtt tgaacgctga cgcgtttgtt tccgctgacg
      781 cggaaacgtg cgggcgggaa accggcgaac cgacttcgcc gaaggccgaa agcgacagta
      841 acggcaacgg cggcctttcg ggaaaaccga aaaatgcgaa tgttccgcgc cgccgcaaaa
      901 cccacggcgt accgcttcag gaaatcgccg atttgtacaa cgaagttttg ggcggccggt
      961 tgccaagcgt ccaagtgctg aacgacacgc gcaaacgggc gattgcaaac cgctggtgcg
     1021 agatgctggg aacggcggcg ccaaacggca aggtgaggtt cggggacaag gaaaccggtt
     1081 tggcctggtt tgccggtttc ttccggaaag tggcgatgaa cccgttttgg atgggcgaaa
     1141 accaaacagg gtttgcggtc ggcttcgatt ggattttcaa ggcgggcaat ttcgtcaaaa
     1201 tccttgaatg gcatccgcct aaaacgaacc aggcggcaag gggaagggca tgaaccgaat
     1261 cgaggaaacg gaagccgtcc aatcactggc cagcgtaggg gcggaacaga acattttggg
     1321 cggcatcttg attgaaccga cggcgattgc gcggtgcgca atcctgaccc ctgaaaagtt
     1381 ttaccaggcg caacacagga ttattttccg cgctctgctg gatatggcgg cggcaaacga
     1441 gcctatcgac atcatcacgc tgaacgacaa gctggaagcg cgcggcgagg cggaaaacgc
     1501 gggtggcctg gcttacttga tagacctgaa ccaaaacacc ccaagcgcga agaatatcag
     1561 ccggtacgtt gggattgtga acgacaggtt tgtcgagcgc ggcttgctga aggcttcggc
     1621 ggcgattgaa aaaatcgcgg tttccaaaga cggcgggacg gtcgcagaaa agctgtctaa
     1681 ggcggccgac gaattggcgg cagccggcaa agacgcggta aagcgtgaaa ccaagacatt
     1741 cggccagacc gttgaggatt tgattggcgg tttggacaaa aggcttgacg gcgtgcgttt
     1801 cggattgcct accggcttga tgaagcttga cgggatgacc ggcggtttgc cggatggaaa
     1861 cctgattgtg attgcggcgc gtccgtctat gggtaaaacg gttttggcgg aaaacattgc
     1921 gcgattcgcg ctgaagcagg gcaaggcagt tcatttccaa agctacgaaa tgagcgcggt
     1981 agagctggca aggcgcggca tggcggctga gtgcaatatc cccatgcaga acctgaaaac
     2041 cggcaatctg acgcaaagcg attacgccaa tatgccgatt tacgtcagcc aagcgaaaga
     2101 gtggaagttt gacgtgaact gcgacctgct caacgttgac gagctttgct ttttggctaa
     2161 ggagaaaaaa ctcactaccg gcttggattt gttggttgtc gatcaccttc acatcatgcc
     2221 aagggcaggg agggacgagg tggcggagtt ggggaatata tcgcgccgtt tgaaaaactt
     2281 ggcggcagag ctgaatatcc ccgtcgtctt ggttgcccag ttgaacaggg gaaacacaaa
     2341 gcaggcagac aaacgcccga acatggcaga cattcgcggc agcggcgcga ttgagcaaga
     2401 cgcaaacatc atcatcatgc cgcaccgcga aagctactac gacggaaacg agaatccgag
     2461 cattgccgag ctgattatcg ccaagaaccg ggacggcgaa acgggaacgg tggtttgcgg
     2521 ctggaaaggg caatttatga agttcgagga agagcctgat ttggcatggc aagcccccaa
     2581 acatgatgaa tatgaccctt acagtgtcta gtgcgggagg ccggtaaatg cgtgaaacct
     2641 gtttctattg caaccatgcc gacttcaaaa ccaacaccg
//
LOCUS       DAWTHD010000155         2607 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWTHD010000155 DAWTHD010000000
VERSION     DAWTHD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.155"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(84..473)
                     /locus_tag="WM704_002047"
     CDS             complement(84..473)
                     /locus_tag="WM704_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFA9341386.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(636..1015)
                     /locus_tag="WM704_002048"
                     /pseudo
     CDS             complement(636..1015)
                     /locus_tag="WM704_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1069..2202)
                     /gene="carA"
                     /locus_tag="WM704_002049"
     CDS             complement(1069..2202)
                     /gene="carA"
                     /locus_tag="WM704_002049"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFA9341387.1"
                     /translation="MNTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAADADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          532 a          651 c          748 g          676 t
ORIGIN      
        1 gcttttcaga cgaccttttt aaacgcttaa ttcagttaat tttaccttat ttttttcaaa
       61 atctaatcaa aatcaaccgt ttgctaccgt gctggctgct tttccaattc ctgcaatacg
      121 cgcaggattt ccgccagtac atcgtttgtc tgctgcaaaa tttcgtgatt ccaaaaacgc
      181 agcacggtaa agcccaagct gttgagatat accgtccgcg cgtggtcgta tacggcttgt
      241 tccgcgtgct gcccgccgtc tgcttcgaca atcagcttgg gcgttacgca cataaaatca
      301 acaatataat tccccatcgg ctgctgtcgg cggaatttat agccgttcag acggcctgcc
      361 cgcaggtgtt gccacaattt tgcttccgcc tcgctcattt cttgacgcat ggctttggcg
      421 cgttggcgca gggcggggtt ttcggcggtc aatagtttct cgggcgggtt catttttgtt
      481 atcgggttgc tgtttgtgta agccttacag cttgttgccc tctctctaac tctttcccac
      541 agggagaggg gacgggtcgg ctgttggggt taaaggggct gcaaggaaaa cagcttgtgc
      601 aggctgtttt ttattttgca taatccgtct gaaaattaat aactgctgat ttccagcagg
      661 ttgccatcgg gatcgcgcag gtaaaccgat tggattttgc ccattgcgcc tgtgcgcgct
      721 acgatgccgc ttaaaggttt gatgccgtgt gcggataatt cctgtaaaac cgtttccagt
      781 ggggcgtatc ggtcagcagg cataaatccg ctgtgccgca ggcggcgtgt tgcgcgttag
      841 gctgaatttc cgcaccgcgc ccgtgtaggt tgattttctg actgccaaac aacaaagctt
      901 tacggttgtt gccaaatgaa acttcttcca tgcccaaaac ttgtgtgtaa aacgcgatgg
      961 ttcggtcaat gtcggcaaca gtcagtacta gatggtcgag tgcgctaatt ttcatttcca
     1021 gctttctttt tcagacggca gcagcatact gttgccgtct gaaaatcatt atgcttgttt
     1081 tgcagccttc atattgccaa tgaatttgtc gaacaaatag ccgacatctt gcggacccgg
     1141 gctggcttcg gggtgtcctt ggaaacagaa cacgggtttg tcggtcagct cgatgccttg
     1201 caaggtattg tcgaacaagg atttgtgggt aatgcgtgcg ttggcgggca gggtgtcggc
     1261 atcggcggcg aaaccgtggt tttggctggt aatgacgact ttgccgctgt ccaaatcttg
     1321 cacaggatgg ttcgcaccgt ggtggctgaa gcgcattttc agggttttgg cgccgatggc
     1381 gaggctgatg agctggtgtc ccaagcagat gccgaaaatc ggtttgccgc tttccatcag
     1441 tttttgcacg gcttcgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggacag
     1501 gaacacgccg tcgggattga gtgccaacac gtcttccgcg ctcgtttgtg cggggacgac
     1561 ggtcaggcgg cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc
     1621 gtaggcgacg acgtggtaag gctgtttgtc aggggtaacg aaacctttgc ccaattccca
     1681 ttcgccttcc gtccattcgt aagtttccgt gcaggaaact tcttttgcca aatctttgcc
     1741 gaccatgctg ccgaacgcgg cgatgagttc ttgcgctttt tcaacggtgg catccgcacc
     1801 tgtcagaatc gcgccgcctt gcgcgccttt ttcgcgcaac agcatggtca ggcggcgggt
     1861 gtcgatgtcg gcgatggcga cggtttcgtt gcgtaccaaa tagtcgtgca ggctttcgga
     1921 ggcgcggaag ctgctgtgca agagcggcag gtcgcggata atcaggcctg cggcgtaaac
     1981 gctgcggctt tcttcatctt cggcgttggt gccggtgttg ccgatgtggg ggtaggtgag
     2041 ggtaacgatt tgtttgcagt aggacgggtc ggtcaggatt tcctgatagc cggtcatcga
     2101 agtattgaac acgacttcgc cggaagccga accttcgtaa ccgattgatg tgccgtggaa
     2161 tacgctgccg tcagcgagga cgaggagggc gggggtgttc atgatgggaa tcctgttttt
     2221 aataaaattc ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa
     2281 atccggtcaa aaaaaaacac gccgcagaac aaaaccgcgt aacgtgcttt tcgggcaagt
     2341 gcctataccc tgaaagcagc gtattctaag acgaaaccta cacgatttca agcgcaaacg
     2401 cggcaggcgg gctgatatgt tcgcgcgaaa acggaaatcc ggggcataaa accgcccgct
     2461 ccgcgccccc tatgcccgtt acgcatttcc ccacgtccgc ccggcgaaac tatgggaata
     2521 cccgaaccgt cattcccgcg cgggcgggaa tccggacttg tcggcacgga aacttatcgg
     2581 gtacaacggt ttcttgagat tttacgt
//
LOCUS       DAWTHD010000156         2583 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWTHD010000156 DAWTHD010000000
VERSION     DAWTHD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.156"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..225)
                     /locus_tag="WM704_002050"
     CDS             complement(<1..225)
                     /locus_tag="WM704_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341388.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSA"
     gene            complement(258..458)
                     /locus_tag="WM704_002051"
     CDS             complement(258..458)
                     /locus_tag="WM704_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341389.1"
                     /translation="MNELISRINRFGARAKDGQSLLLKVGEICRDAAATWTTRKSESI
                     NHTAFTFTVKKDGLKEKVMIVL"
     gene            complement(656..1069)
                     /locus_tag="WM704_002052"
     CDS             complement(656..1069)
                     /locus_tag="WM704_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341390.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(1066..1527)
                     /locus_tag="WM704_002053"
     CDS             complement(1066..1527)
                     /locus_tag="WM704_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9341391.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1544..1981)
                     /locus_tag="WM704_002054"
     CDS             complement(1544..1981)
                     /locus_tag="WM704_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341392.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(2096..2443)
                     /locus_tag="WM704_002055"
     CDS             complement(2096..2443)
                     /locus_tag="WM704_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFA9341393.1"
                     /translation="MDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIENGDVMFV
                     DTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGEDLNAVRI
                     IGRIRGAWRLSQF"
BASE COUNT          570 a          574 c          632 g          807 t
ORIGIN      
        1 tgccgaataa gtgcggatga tgacttcctt gccgatggca aagtcgttga tacctttttg
       61 ctcggcaact gttaccggcg gttgcggctc atgttgcggt tcgtcaaatt cggttgaaat
      121 gtcggcgcgt tttacaccca ttgccgcttc gaaatcggca gcaatgcctg caaagacttt
      181 tataaggtct gacaaacttt tcacttcaaa tttatttgct tccatttttg tttcctttcg
      241 gggtggggtt ggtttcttta caaaacaatc attaccttct cttttaagcc gtcttttttc
      301 actgtaaaag tgaaggcggt gtgattgatg ctttcgcttt ttctagtggt ccatgtcgct
      361 gctgcgtcgc ggcagatttc gccgactttc aataaaaggc tttgcccgtc ctttgccctc
      421 gcgccaaacc gatttattct gctgattaat tcgttcatcc cgtttccttc aagttgttgt
      481 ttgtttcgat gggtgtatta taactattac ctatatttaa tcaataggca atagttatat
      541 tttcatccat atatacttat tgaattgatt tttagaagaa aaaagtttga aaaaaaaccg
      601 cccgaatggc ggtgtatcat gaaaaagaaa accgccctag ggcggttcgg tattgttatg
      661 agtggtaagg aagccaagaa aaagacaaag gacggctctc ggagagaata tggttgtgtg
      721 ttgtcattct tacataaatg cggtcgttgt ctgaaacggg tagtccgctg attgtttggt
      781 gtagcatgaa tgacacgttt tcttctgtat cactgccgtc atatgatgga atctcaagtg
      841 cagagattac atcatcgttg cggacgattt ccagaaaaat ttgaccggtt ttcatagtct
      901 gtatatccat gccttcggcg gaaaatgaaa cagacaggca caggcggcct agaatggcct
      961 gcggtgtcgg aatatggcat atatcgggga atatacctat caggctgtat ttgtcatttt
     1021 ccgcatggcg gataatttca tcgcaatagt ggacatttaa gtgaatcatg cctgctccat
     1081 atattcgtac cgccgttcga acgctgcgcg aacttcaagc ggggaaacgc ctaatgcgtt
     1141 tgccaatttt tgtacagtct tatcttgcag ggattgtttg ctgttttcga tgcgggacag
     1201 atagggttga ggcaatcctg cggcggttgc caattcggac tgggtaaacc cctttttcat
     1261 ccttaagctg acaaatgttt ccccgcctgc tttgagggct attttatcgg caattcttgc
     1321 tgccgccctg tccatcgctg ctttgcggcg cggatttttc cgcacatgct caatatgctc
     1381 tgcggcaggg gtagtgttag ggcttatcgg tttgacgcat ttaatctgcc cgtttatccg
     1441 caggcatttt atgtgggtga cggggatggc ggatgtaggt gttgatactg tgcaggcggc
     1501 cacggcaaca gtgagtgttg cacccatttt gaaccatgtg ccgttagttg gagtacagtt
     1561 cttcatagtc tttgataatc cgttttgtga ttgggtgttc tgcctgatag tcaaattcgt
     1621 ctgtcttctt gtttacaaca gccaatatat ctatgcggcg gatatacgtg ccgtttggct
     1681 gtttttcatg gcatggtgcg taaattattc tgtatccggc ggcttcttcg ctgtcaaatc
     1741 ggacgcgtaa aactttgatg tcttttcccc ataatgacaa tatgggtttc acttccaagt
     1801 caatcgggcc tatgggttcg ccatattccc tgaagtatct gtttttgtat aagccgtcaa
     1861 aaatagcaga gtccgcctga atcatcgcaa tgacattctc gagataaccg accgcttctt
     1921 cgtctttttc aaagaggcgg tctaaatcct gctcggcgtt aaagtggacg gtcagctcca
     1981 tgagtgccct ttttatatat atatattata tctttttagt tataccgcgc aatatgcggt
     2041 aataaatcag ttgttcaagt attttaatcc ggcacgctcc accagaagac gcggtctaga
     2101 actggctcaa acgccatgcg ccgcgtatgc gtccgatgat gcgtacggcg tttaaatctt
     2161 cgccgcgcac ggtttcggtt cggtatgagc tgttgtcgct gatgatcatc aggccgccgc
     2221 cgacggtgga ttgcagccgc ttggccttaa ggccgtctat ataccaaagc aggtagaggc
     2281 cgtcgccatc gaaggcttcg acggcggtat caacgaacat tacgtcgccg ttttcgatgg
     2341 tgggctccat gctgtcgcca cgggctgtaa tgacttggat tttgttgagg tttccgccca
     2401 gtttctcccg cgcccatgcg gcagcgacgg ttacataatc cacaacctcg atatagtggt
     2461 cgttaatcgt gcctgcgccg caggtcgctt cggcatttaa gcgggggaaa cgtatgctac
     2521 tttgttcatt cgattttaag actctgaatg tttcaatgcc gaaatggctt ggcgttacta
     2581 cgt
//
LOCUS       DAWTHD010000157         2530 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWTHD010000157 DAWTHD010000000
VERSION     DAWTHD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.157"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..307
                     /locus_tag="WM704_002056"
     CDS             <1..307
                     /locus_tag="WM704_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HFA9341394.1"
                     /translation="NQAETEGVWVHNACPPRKTPSTPVYGNDSEAYAAAKKLGYRKIK
                     ERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDKNLNRIGD"
     gene            313..711
                     /locus_tag="WM704_002057"
     CDS             313..711
                     /locus_tag="WM704_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9341395.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDAEDWKFFYFPIQHSKGRLR"
     gene            742..1119
                     /locus_tag="WM704_002058"
     CDS             742..1119
                     /locus_tag="WM704_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9341396.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            1250..1987
                     /locus_tag="WM704_002059"
     CDS             1250..1987
                     /locus_tag="WM704_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9341397.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGNRLFAESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            1984..2403
                     /locus_tag="WM704_002060"
     CDS             1984..2403
                     /locus_tag="WM704_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFA9341398.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          877 a          425 c          540 g          688 t
ORIGIN      
        1 taatcaggcg gaaacggaag gggtttgggt tcataatgcg tgtccgccaa gaaaaacacc
       61 atctaccccc gtatatggga atgattcaga agcatatgca gcagcaaaga aattgggata
      121 tagaaaaata aaagaaagaa cgagaaatga tgctgctatt tttaaaaaag gaaagagtta
      181 tatatcacgc gatgtagatt cacataatgg aggggcttgg aaagaagcat cttcacccaa
      241 aaatctgaac agaaaagaaa caagaaacgg tacatttgat aaaaacctaa atcgtattgg
      301 agattaatta ttatgtgcga gttcaaggat tttagaagaa acatcccttg ttttgaagag
      361 tatgacgaaa attcatttat tggcaaatgg tatgatgacg gagtgtggga tgatgaagaa
      421 tattggaaat tggagaatgc tttaatcgag gttagaaaaa aatatcctta tccgatggat
      481 ataccaaggg acatcgtgat tggaatcggt accattattg attttttaat ggttcaaaat
      541 tggaaacttt ttgaaattaa agcttctcct tggttgccta aaagtgtcaa aattaatgag
      601 cgttatgaaa ggttcagagt aatgctccgt tatattttta ctgatctaga tgcagaggac
      661 tggaaatttt tttactttcc aatacaacat agtaaaggta gattgagatg aaaagtttaa
      721 ttatattgga gatacattat tatgtgtgag ttcaaggata ttataagaaa cgttccttat
      781 tttgaggggt atgacgaaaa ttcatttatt ggcaaatggt atgatgacgg ggtgtgggat
      841 gatgaagaat attggaagtt ggagaatgat ttaatcgagg ttaggagaaa atatccttat
      901 ccgatggata taccaaggga tatcgtgatt ggaatcggta ccattattga gtttttaatg
      961 gttccaaatt ggaaactttt tgaaattaaa gcttcccctt ggttacctga tagcgtggga
     1021 attaacgaac gttatgaaag gcttaaaaca atgctccgtt atatttttac cgagaaagac
     1081 atagtcaacg tgcaatttga ttattacaac aaaaaatagc aaaacccggc aatcaaaatg
     1141 ccgtctgaaa ccgtgtttaa ttttcaggcg accttcccct tattttaaac aggatattgg
     1201 gaactaagtt cttcaaaaat cctacacctg ctctttccac ggcagcacct tggtcagaac
     1261 ggcagacggc tacaaagcca tcgcccatat tcaagccgga gaccgcgtcc tttccaagga
     1321 cgaggcaagc gggaaaacag gatacaaacc cgttaccgcc cgatacggca atccgtatca
     1381 agaaaccgtt tacattaaag tttcagacgg catcggcaac agccaaaccc tgatttccaa
     1441 ccgcatccac ccgttctatt cggacggcaa atggattaag gcggaagatt taaaagcggg
     1501 aaacaggctg tttgccgaaa gcggcaaaac ccaaaccgtc cgcaacatcg ttgtcaaacc
     1561 aaaaccgctc aaagcctaca atctgaccgt tgccgactgg catacctact ttgtcaaagg
     1621 cgataaggcg gaaacggaag gggtttgggt tcataatgat tgtccgtatg gcggttcaaa
     1681 taatttagag aaagcaaaac ttcgcgcaga acggcttagt aaaaacgatc gggcaggaaa
     1741 agactttact aaagcaggaa aagaagccgt tattgatttg aataggattc aaaataatgg
     1801 tcaagttaag tgcgctaatt gcggtattga aacaattcca gccaaacaat ctataaaaaa
     1861 tatttctcct acttcaaatg aaagacaagt agatcatgtc atccctaaat ctaagggtgg
     1921 tcaaggtaca cctaaaaacg ggcaggtatt atgcagaggc tgcaacatta agaagagtaa
     1981 caaatgaaat cgaaacttac tgtcgtctat tacgatttgg aaagcaatat tgcagaagaa
     2041 atactgtcag gaaacataat gcctgatgga aattttctta ttcaagagat tcctcttttc
     2101 gcaccgaatt tggctttaaa cgacattgtt gccatagaac gtgaggataa gatgctgttt
     2161 ttcgaccact tgataaaagc ttcaggaaat accacgataa acatcgttgt tttggatcat
     2221 ttcccaaagg atttattggc agccatagaa gaacacagtg gtaaaatcag aaaaaatgga
     2281 gagaattatt tatcggttaa ttttccgccc aaaaaatata attctgattt aaaaggaatt
     2341 ttaaatagat atgaggaagc aaatatcctc agctacaggg aagcttgttt gggcttctct
     2401 taatcgatct ataagtaaga atggattacg ttagattgta aacctaacga ttaaaaggtc
     2461 gtctgaaacc gtgtttaatt ttcagacgac ctatttcctt catttgaaac aggatattgg
     2521 gaattaagtt
//
LOCUS       DAWTHD010000158         2435 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWTHD010000158 DAWTHD010000000
VERSION     DAWTHD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.158"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            41..322
                     /locus_tag="WM704_002061"
     CDS             41..322
                     /locus_tag="WM704_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9341399.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            382..1152
                     /gene="xth"
                     /locus_tag="WM704_002062"
     CDS             382..1152
                     /gene="xth"
                     /locus_tag="WM704_002062"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFA9341400.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(1426..2103)
                     /locus_tag="WM704_002063"
     CDS             complement(1426..2103)
                     /locus_tag="WM704_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFA9341401.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
BASE COUNT          522 a          652 c          715 g          546 t
ORIGIN      
        1 tcaatccgct tggcgggcgg aaaaggtttt aggaataccg atgaacacaa taccgctcca
       61 caccatactc aaacttatgg cgcatcccga acgtatggcg atactgatcc aactgttgga
      121 cagcgaacgc aatatcgtcg aactggcaaa atccttatcc ctgtcggcca ccgcggtttc
      181 ctgtcatttg aaccgcctga gggcgggcgg tctggtggac tttacgcgtt accaccgcat
      241 tatcgaatac cgcctggttt ccgaagacgc ggcaacgatt ctgcggacaa tccgcgattt
      301 ggaaaacaaa cgcgcggcat agtgttagaa tcctttcctt ttgccgtctg aacgtttcag
      361 acggcatttt tcgaaaatgt tatgaaaatc accacttgga acgtcaattc gctcaatgta
      421 cgtctgccgc aggtgcaaaa cctgcttgcc gacaatccgc ccgatatttt ggttttgcag
      481 gaactcaaac tcgatcagga caaatttccg gccgccgcct tgcagatgat gggctggcac
      541 tgtgtttgga gcgggcagaa aacctacaac ggtgtggcaa tcgtcagccg cagcgtgccg
      601 caggacgtgc atttcggttt gccttccctg ccggatgacc cgcaacgccg cgtgattgcg
      661 gcgaccgtag gcggcgtgcg cgtcatcaat gtctattgcg tcaacggcga ggctttggac
      721 agccccaaat tcaaatataa ggaacagtgg tttgccgcac tgacggagtt tgtccgcgac
      781 gaaatgaccc gccacggcaa actggtactg ctgggcgatt tcaatattgc gcctgccgat
      841 gcggactgtt acgaccctga aaaatggtat gaaaaaatcc actgttcgtc cgtcgaacgg
      901 cagtggtttc aaaatctgct ggatttggga ttgaccgaca gcctgcgcca agtccacccc
      961 gaaggcgcgt tctatacctg gttcgactat cgcggcgcga tgttccaacg caaactgggc
     1021 ctgcgtatcg accatatatt ggttacatcc gaaatggcgg ccgttttgaa ggatgtccgc
     1081 atcgatttgg agacgcgcgc gctggagcgt ccgagcgacc acgcgcccgt ggcggcagaa
     1141 tttgatttgt aattcgggaa aggcaaatgc cgtctgaaac ttcgcagggt ttcagacggc
     1201 attttgattg aatatctgtt taaacgatga ggttacaggg gtatcgcggt atggcggtaa
     1261 cgtctgcaag gttgggccca cttgcacgcc accggctgca atctgcggtt tcccggcaaa
     1321 cggcgagacg ttatccatag cattacagtc ggggtacggc tgtgcttgcg aatacggaaa
     1381 tgccgtctga aaaacattca gacggcattg gcgttgcagg gcgcgttaca tgtgccgtgc
     1441 ggcgttgacg gcgcgttggt cggggttgat gaggatttcg acgcggcggt tttgcgcgcg
     1501 gccttcaacc gtagcgttgg acgcgaccgg catatgcgaa ccgtagccgt aaaccgtcag
     1561 gcgcgaagcc gccacgccgc gcgtctgcag atagtaggca accgcttggg cgcggtgttg
     1621 cgaaagcgga ttgttgactg catcggaacc tgtgttgtcg gtgtgcccgt tgatggtcag
     1681 cgtcgtgtcg ggatactgca ccagcgtctg tgcggcagtg ttcagggcgt attgcgcact
     1741 gccgcccaac gccgcgctgc cggtggcgaa ggtaacgctt tcgggcatca ccagcctgat
     1801 ttggttgcct tggcgttgga tttcgatttg cgtgccggca aggttttggc gcaaacgctg
     1861 ctcttggtag tccatatagc cgcccacgcc tgcgccgatt gcgccgcagg caagcgcgga
     1921 attgcgtgcg cctttgccgc tgtgggtcag tgcgccgacg atgccgcaca ctgccgcgcc
     1981 acccaaaccg tacatggcgg atttgtttgg ggactgctgt ccggttacgg ggtcggcaac
     2041 gcagccggaa agcgcgagtg cggatgcggt cagcacgacg gtaaagggtt tgaagaaagt
     2101 catggcgttt ccttccgtgc atatgtcaaa ccgtacaact ataagcaatc atgccgtctg
     2161 aaaaagtgca attaacctga aattacccga tttttcccga aacaacgcgg gtttatcgtg
     2221 atttgcgccg aaaccgcagc cacatccgca aaaaaacgta ccttcacatc gtagccgacc
     2281 gagcctgcac ccggtatcgg caataatgcc gaaacgggca gggagaaatg gtgtccgatt
     2341 ttatggcagc cccacgcgcc gacgatgccg ccgagcagcc atattttcgg tttgtcgacg
     2401 tgtacgtgcg gcaggcgcgg atggtgggtt tagga
//
LOCUS       DAWTHD010000159         2427 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWTHD010000159 DAWTHD010000000
VERSION     DAWTHD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.159"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(187..747)
                     /locus_tag="WM704_002064"
     CDS             complement(187..747)
                     /locus_tag="WM704_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341402.1"
                     /translation="MDIKTEEAYRLSFQHIQSHDVLAACRTVANYELSQPRPRGLFSG
                     ISAQEYWESYPCNEDAEILKSILLDSPEILDGISGGDMEMARIMAAFNFIWGLSHFPK
                     WLHRHEFASTPLDDSAVPAMLLFHAQSRQELKEYEEVELSCCPDGCEFCKSEDGKIYK
                     SSDAPVLPHAHCTHEQGCRCCYLPVI"
     gene            complement(747..1445)
                     /locus_tag="WM704_002065"
     CDS             complement(747..1445)
                     /locus_tag="WM704_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9341403.1"
                     /translation="MNRVDRIKNLIADRFNGNQAEFSRAINKAPAQINQWLNGYRNIG
                     DGVAAQIEIALGLPRGWVDGNDKPGIPVDSIKSNATVIGSVDAWDSKTPLSDDDCEVP
                     FYKDVCLSAGNGFSDEIEDYNGYKLRFSKSTLRRHGINPDDVVCVSADGDSMEPVFPD
                     GATLGINTADKMIKDGKIYAINHSGLLRTKILQKLPDNQVRIKSYNPEYKDETASLDS
                     LTVIGRVFWWSVLD"
     gene            1559..1789
                     /locus_tag="WM704_002066"
     CDS             1559..1789
                     /locus_tag="WM704_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9341404.1"
                     /translation="MDLRDYCAIRGNQSDLARKTGISPSFIHQIAKGLKPVPIQSAAL
                     IEKSTNGRVTRKEMFPDTWHLIWPELADDQPK"
     gene            1892..>2427
                     /locus_tag="WM704_002067"
     CDS             1892..>2427
                     /locus_tag="WM704_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFA9341405.1"
                     /translation="MKYIPNSFQIANAVVDDFLCRMSGNAWKCYAVIVRKTTGWQKEI
                     DYISVSQFKNLTGIKTDVTVADALKELVELNLIASVKRHGQVTGYRINMPEPSPENGG
                     TPPPENGGTATPKNWVHPKNGTTPKNWGVLPPENGGTTTPKNWGSTKHTTKPTNTKHS
                     ISASAAADAPLSAEPPETA"
BASE COUNT          673 a          592 c          549 g          613 t
ORIGIN      
        1 tttcgcaggc acaaaaaaac cgcctttcgg gcggcttttt tacgaacata aaaaaattat
       61 aaagtcaagg aagcaccatg ttgacttcct gaaatcacca tgccgatttc ctaacaccgg
      121 cgggcaagcc tacatgatgt tcattttttg ggtattaaaa aagcccgcat cgcgcgggct
      181 gtgtttttaa ataacaggca gatagcaaca tcggcaaccc tgctcatgcg tgcagtgtgc
      241 gtgtgggaga acgggtgcgt ctgatgattt gtatatcttg ccgtcctccg atttacaaaa
      301 ttcacaacca tcggggcaac aagacagctc gacttcttcg tactctttta attcctgacg
      361 cgattgtgcg tggaaaagca gcattgcagg gacggcagaa tcatcaagag gcgttgatgc
      421 aaattcatgc cggtgaagcc atttcgggaa atgactcaat ccccaaataa aattgaatgc
      481 agccatgatt cgcgccattt ccatatcgcc gccgctgatt ccgtccagta tttcagggga
      541 atctaaaagg atagatttca agatttcagc gtcctcattg cacgggtagc tctcccagta
      601 ttcttgagcg gatatgcccg aaaacagccc gcgcggtctt ggctgcgata attcataatt
      661 tgcaaccgtt ctacacgccg ccaatacgtc gtgtgattga atgtgttgaa agctcaatcg
      721 atatgcttct tctgttttaa tatccattag tctaatacac tccaccaaaa taccctgcca
      781 ataacggtta ggctgtctaa agaggcggtt tcgtctttgt attccgggtt gtagctcttg
      841 atacggactt gattatcagg cagcttttgc aagatttttg tacgcaacag cccactgtgg
      901 ttgatggcat agattttgcc gtctttaatc attttatcgg cagtattaat acccagtgta
      961 gccccgtcgg ggaataccgg ctccatactg tctccgtcgg cagatacgca gacaacatcg
     1021 tcagggttta tgccgtgccg tcttaaggtt gattttgaga aacgcagttt atagccgttg
     1081 tagtcttcaa tttcatctga aaagccgtta cccgccgaca aacaaacgtc tttataaaat
     1141 ggaacttcgc aatcatcgtc ggataacggg gttttgctat cccaagcgtc aacagagccg
     1201 ataacggtgg cgtttgattt gatggaatca acagggatac ccggcttgtc gtttccatcc
     1261 acccatccgc gcggtaatcc aagcgcgatc tcaatttggg cggctacgcc gtcccctata
     1321 tttctgtatc cgttaagcca ttgatttatc tgggcaggag ctttattaat tgcccgagaa
     1381 aactcagctt gattgccgtt aaacctgtcg gctatcagat ttttaattct atctactcgg
     1441 ttcatttcat taccccttca aataggtata aatataaagc atagctacat ttatcaatgc
     1501 tactatttta tatttagcta tgctatactc acgctaaatt gatttgacag agatcgaaat
     1561 ggatttaaga gattattgcg cgatacgcgg caatcagtct gatttggcaa gaaaaacagg
     1621 aatttccccg tcttttatcc atcagattgc caaaggattg aagcccgtcc caattcagtc
     1681 ggcggcgttg attgaaaagt caacaaacgg cagggtaaca cgaaaagaga tgtttcctga
     1741 tacctggcat ctaatctggc cggaattggc agacgaccaa cccaaataaa aagcccgtcg
     1801 gggatgacgg gcagccggtt acgcattact cgattgttga aaacggggtg gaagtgtggg
     1861 cgcaagacca atgggaatgg aaggacggta aatgaaatat attccaaatt cgtttcagat
     1921 agcaaacgcg gtagtggacg atttcctctg ccgaatgagc ggcaacgcgt ggaaatgcta
     1981 cgccgtcatc gtgcgcaaaa cgaccggctg gcaaaaggaa attgactaca tctctgtttc
     2041 ccaatttaaa aacctgaccg gaatcaaaac agacgtaaca gttgccgacg cgctgaaaga
     2101 gcttgtggaa ttgaacctga ttgcctccgt caaacggcac ggtcaggtaa ccggctaccg
     2161 catcaatatg cccgaaccgt cccccgaaaa tgggggtaca ccacccccgg aaaatggggg
     2221 tactgccacc cccaaaaatt gggtacaccc aaaaaatggg actaccccca aaaattgggg
     2281 ggtactaccc ccggaaaatg ggggtactac cacccccaaa aattgggggt ctacaaaaca
     2341 cactacaaaa cccactaata caaaacacag tattagcgca tccgcagcgg cggacgcgcc
     2401 cctttctgcc gaacctcccg aaaccgc
//
LOCUS       DAWTHD010000160         2411 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWTHD010000160 DAWTHD010000000
VERSION     DAWTHD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.160"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(94..1728)
                     /gene="groL"
                     /locus_tag="WM704_002068"
     CDS             complement(94..1728)
                     /gene="groL"
                     /locus_tag="WM704_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFA9341406.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            complement(1821..2111)
                     /locus_tag="WM704_002069"
     CDS             complement(1821..2111)
                     /locus_tag="WM704_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFA9341407.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          448 a          661 c          645 g          657 t
ORIGIN      
        1 tttaaggttt gcgccttagc cgcacttatt cgcaaaaaaa ccgcacggcg ttgaccgtgc
       61 ggcttgttgt ctgaaggttt cagacggcat tgcttacatc atgccgccca taccgcccat
      121 tccccccata tcgggcacag ccggtttttc ttcagggatt tcggcaatca tgcagtcggt
      181 cgtcagcatc agaccggcga tagacgcggc gtgttgcagc gcggaacggg ttactttggc
      241 agggtcgagt acgcccattc cgatcatgtc gccgtattcg ccggagcctg cgttgtaacc
      301 gtagttgcct ttgccttcca acactttgtt caccaccacg ctgggttctc cgcctgcgtt
      361 ggcaacgatt tggcgcagcg gagactcaac ggcgcgcaat acgatttgta cgcctgcgtc
      421 ttggtcggca ttgccggtgt gcaggttttc caaagcggca cgggcgcgca acagggctac
      481 gccgccgcct gcaaccacgc cttcttcaac ggctgcgcgg gtagcgtgca gcgcgtcttc
      541 cacgcggtct tttttctctt tcatttcgac ttcggtcgcc gcgccgactt tgatcactgc
      601 cacgcctcct gccagtttgg caacgcgctc ttgcagtttt tctttgtcgt aatcgctggt
      661 cgcggtttcg atttgttggc ggatttcggc aacacgcgct tcgatttggg ctgcgtcgcc
      721 gaagccgtcg atgacggtag tgttttcttt accgatttcg atgcgtttgg cttgacccaa
      781 gtcgtccaaa gtcgcttttt ccaaagacag gccgacttct tcggaaatca ctacgccgcc
      841 ggtcaggatg gcgatgtctt gcagcatcgc tttgcggcgg tcgccgaagc cgggggcttt
      901 gacggcaacg gttttcagga tgccgcggat gttgttcacg accaaagtcg ccaaggcttc
      961 gccttctacg tcttcagcga taatcaacag cggacggctg gctttcgcca cttgttccaa
     1021 cacgggcagc aggtcgcgga tgttgctgat ttttttgtcg aacagcaaaa caaacggatt
     1081 gtccagaccg gcgatttgtt tttccgcgtc gttgatgaag taaggggaca ggtagccgcg
     1141 gtcgaactgc ataccttcaa ccacgtccag ctcgttttcc aaagatttgc cgtcttcaac
     1201 ggtaatcacg ccttctttgc cgactttttc catcgcttcg gcgataatcg cgccgacttg
     1261 ttcgtcggag ttggcggaaa tcgagccgac ttgggcgatt tctttggaag tatcgcaagg
     1321 tttggcgatg tttttcagct cgtcaaccaa agcggcaacg gctttgtcga tgccgcgttt
     1381 cagatcggtc gggttcatgc cggcggtaac gtatttcatg ccttcggcaa cgatggattg
     1441 tgccaatacg gtggcggtag tcgtaccgtc gccggctacg tcgttggttt tggacgcgac
     1501 ttcttttacc atttgcgcgc ccatattttc aaacttgtct ttcagttcga tttctttggc
     1561 gacggttacg ccgtctttgg tgatgtgcgg gccgccgaaa gcgcggtcaa ccaccacgtt
     1621 gcggcctttg gggcccaagg ttacgcgcac ggcgtttgcc aaaatattca cgccgtttac
     1681 cattttttgg cggacttcat tgccgaattg tacgtctttt gctgccattt caattctcca
     1741 aaaatcatta aaactgtctg ataaaaccgt ttatgccgtc tgaaggcggt ttgccgtttc
     1801 agacggcatc gtgtccgtat ttatttttca acgatgccga aaatatcttc ttcgcgcatt
     1861 accaacagct cttcgccgtc ggcttttacg gtttggccgc tgtatttgcc gaagatgatt
     1921 ttgtcgccgg ctttgacatc cagcggacgg cgcgcgccgt ctttgccgat tttgcccgcg
     1981 cccacggcga tgacttcgcc catatcgggt ttttcggcgg ccgcacccgg caggacgatg
     2041 cccgatgcgg ttttttcttc agcttccaag cgtttgacga cgacgcggtc gtgtaaagga
     2101 cggatggtca tatttatgct ccgataaatg gtttgaaaac aatcatctgc ccgaacggtt
     2161 cggacaaatt gaagtggaaa ccgggctgcc gcctaacggc ctgcccgtac aagtcggcaa
     2221 aattagggtg tgtgcgggaa aattcaagtg aggcggaaaa aaatttattt ccgccgtttt
     2281 ttatagtgga ctaaatttaa accagtacgg cgttgccccg ccttagctca aagagaacga
     2341 ttccctaagg tgctgaagca ccaagtgaat cggttccgta ctatctgtac tgcctgcggc
     2401 ttcgccgcct t
//
LOCUS       DAWTHD010000161         2356 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWTHD010000161 DAWTHD010000000
VERSION     DAWTHD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.161"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(112..621)
                     /locus_tag="WM704_002070"
     CDS             complement(112..621)
                     /locus_tag="WM704_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFA9341408.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            complement(701..776)
                     /locus_tag="WM704_002071"
     tRNA            complement(701..776)
                     /locus_tag="WM704_002071"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(741..743),aa:Lys,seq:ttt)
     gene            995..2074
                     /gene="apbC"
                     /locus_tag="WM704_002072"
     CDS             995..2074
                     /gene="apbC"
                     /locus_tag="WM704_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFA9341409.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPLTGDTHIHLSIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTATANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT          570 a          780 c          585 g          421 t
ORIGIN      
        1 catcggcagg ggcgccggct ctttcccaaa aaacaaaagc cgccgcatcc gccgcgcaag
       61 gcatatgccg tttgattctc tacatagcgg aaagtttgac aaaaacaaaa gttaaccgaa
      121 aaaatccgcc tgaaaaattc gtgcgcgcaa gccccaataa ctgctgattc ccgtcgtata
      181 gcggaccatt ttcccatttt tgaccaaaac gacggcaggc gttgcggcaa tccgccaaga
      241 ccttgccaaa cccccgtcct catcgctgac agtcggaaag cccaagcccc gttttgccat
      301 atatgccgcc acttcggacg cgctgccgga acgtaccgcc acgccgacga ccggcacgcc
      361 gtccgccgcc aaatcatcga ttatcggcga ctgataacgg cacacgccgc accagcttcc
      421 ccaaaaatac accagcaccg tcctatcccg gctaaactgt cccaaagtca gccgctgccc
      481 cgacagcagg gtcaaaggcc gccctgccgc cccggcaggt tcgtcgggct tgcgtaccca
      541 atccaaaaac agcgacacca gcaaaaacac caatgccgtc cgaacggcaa atttgatgcc
      601 cgaaagcagt ttctttttca tacacccccg caaacggtac gcccgcgcaa ccggcaggca
      661 aacaaaaaag ccaagtctcg aaacttggct tccggttatc tggtgggtcg tgagcgattc
      721 gaacgctcga ccaacggatt aaaagtccgc tgctctaccg gctgagctaa cgacccgata
      781 agccgtgcat tatacagccc cgttccccgc cgtcaagcaa attttacagg cttaattgca
      841 gactgctgtt tgcacgggat attttgacaa cggattttca caatccgccg cataccgtgt
      901 aaaagttcgc acaagaaaaa agcaaaccgc ccgaaatcat gtacactttc cgtctatctt
      961 cccctcacac gacttgcgaa cagaggcaca catcatgaat atacaaaaca tccgcaccct
     1021 cctcgacacc gtcgccgttc cgaatacggc acgcacgctc ggcggcgaaa aggccgtccg
     1081 ctcggtcgga cagcgttcag acggcatcca catcgccctg catttcggct tccctgtcgc
     1141 gcacatcgcc gccgccctcg ccgatgccgt acaagaaacc ctgatgccgc tgacaggcga
     1201 cacacacatc catctgtcca tagacaccga aatcggcaca cacaaagtcc ggcccggcgt
     1261 cgccaccatc aaaggcgtga aaaacatcat tgccgtcgca tcgggaaaag gcggcgtggg
     1321 caaatcaacc gccactgcca accttgccgc cgcaatggcg cgcatgggcg cgcgcgtcgg
     1381 cgtgctcgat gccgaccttt acggtccgag ccagcccacg atgctgggcg tacacgaccg
     1441 caaacccgat cagaaaaacc aaaaactcat tcccgtcgaa tcttcagacg gcatacaggt
     1501 tatgtccatc ggctttctag tcgataccgg ccaagccgtc gtctggcgcg ggccgatggt
     1561 cagccaagcc ttgcagcagc tgatgttcca aagcgagtgg gacgaagtgg actatctttt
     1621 catcgacctg ccccccggca cgggcgacat ccaactcacg ctgtcccaac gcatccccgt
     1681 aaccgggtcc gtcatcgtaa ccacgccgca ggacatcgcg ctgatagacg cgcgcaaggc
     1741 tgtggatatg ttccgcaagg tcaacattcc cattttgggc gtattggaaa acatgtccgt
     1801 ccacatctgc tccaactgcg ggcatagcga agcactgttc ggcacggacg gcggcaaaga
     1861 tttggcggcc cgcctcaacg tccccctgct cggacagctt cccctaagcc tgcccgtgcg
     1921 cgaagccatg gacggcggca caccggcgca actgttcgac gaacaccccg ccatcgcccg
     1981 aatctacacc gatgccgcat tccaaatcgc cctgggcatt gccgacaaag gcaaagactt
     2041 cagcagccgg ttccctaaaa ttgtcgtcga ataaagccgc gcccgaaacc gcaacagcaa
     2101 tgccgtccca agccccgcgc ctgccgacgg gcttcatagt cctctctccc gtgggagagg
     2161 gctggagaga gggcagcaag ccgcaaggct tgtatttggg cggttaagtt gttgggaaag
     2221 gttgccgcaa cttggagaat gccctctccc cggccctccc ccacggggga gggagtgggt
     2281 tgcggcaggt tttgcggttg caggcggttt gaaaaacaga ttggatttac aaccgttgat
     2341 gcaggtcgtc tgaaaa
//
LOCUS       DAWTHD010000162         2278 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWTHD010000162 DAWTHD010000000
VERSION     DAWTHD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.162"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..146
                     /locus_tag="WM704_002073"
     CDS             <1..146
                     /locus_tag="WM704_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341410.1"
                     /translation="LRLLQQTACRFKAALETAAWKHYVRAIKESEPVPDAEARRKRKK
                     QAA"
     gene            complement(422..506)
                     /locus_tag="WM704_002074"
     tRNA            complement(422..506)
                     /locus_tag="WM704_002074"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:tag)
     gene            657..1331
                     /locus_tag="WM704_002075"
     CDS             657..1331
                     /locus_tag="WM704_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341411.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1324..1917
                     /gene="ribA"
                     /locus_tag="WM704_002076"
     CDS             1324..1917
                     /gene="ribA"
                     /locus_tag="WM704_002076"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFA9341412.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2085..>2278
                     /locus_tag="WM704_002077"
     CDS             2085..>2278
                     /locus_tag="WM704_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9341413.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIRVIPCVT"
BASE COUNT          556 a          523 c          648 g          551 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccgatg
      361 atgtgatttg caccacattt gcaccaagtg gcatataaca ttatgaaaga tataaaaatt
      421 ttggtgcgga cggagagact cgaactctca cacctctcgg cgccagaacc taaatctggt
      481 gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt ttgtccgatt
      541 gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa atccgctatt
      601 cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat ttgggtatgc
      661 ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg cttgacgagg
      721 ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct tcgattgacc
      781 gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg ccgtgcgatg
      841 atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg ttttcacgct
      901 cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac gccgccctcc
      961 ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag caggatggaa
     1021 atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg aatgttgcct
     1081 cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat catgcttcgg
     1141 cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc gtggttcatg
     1201 tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg gtggctcttg
     1261 ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca gaaggagatg
     1321 aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga atggggcgta
     1381 tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct gaccgtcggc
     1441 aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct gacgggcgac
     1501 gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat gagggcggta
     1561 caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg catcgggctg
     1621 attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt tgaagccaat
     1681 ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc tatctacgaa
     1741 tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat ccaaaccctg
     1801 aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga aaatctggaa
     1861 aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc ggaataaggc
     1921 aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc catagcttga
     1981 taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc gagcgcgggt
     2041 ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt ttctctatca
     2101 tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc gtgcttgccg
     2161 aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac ggctgcggga
     2221 agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca tgcgtaac
//
LOCUS       DAWTHD010000163         2243 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWTHD010000163 DAWTHD010000000
VERSION     DAWTHD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.163"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(65..375)
                     /locus_tag="WM704_002078"
                     /pseudo
     CDS             complement(65..375)
                     /locus_tag="WM704_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            262..774
                     /locus_tag="WM704_002079"
     CDS             262..774
                     /locus_tag="WM704_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFA9341414.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            854..1933
                     /gene="aroB"
                     /locus_tag="WM704_002080"
     CDS             854..1933
                     /gene="aroB"
                     /locus_tag="WM704_002080"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFA9341415.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT          536 a          707 c          580 g          420 t
ORIGIN      
        1 taattgttta cctaagcaaa gataattgcc ttttcctgac aaattaagtg aaatcaacgg
       61 attgtcaaaa cacagcctga aataaaaaac ctccctgatt tcttttattt ttccttaaaa
      121 tcagaaaagt tcgggatggt cgggttattt ttccaaacgt accgccgccc tgccgatttc
      181 gtataaaatc ccgccgtaac ccgacaagcc cgaaccctgc cgccccgaaa ggcggggggg
      241 gtcaaacatt aaggaaccgt gatgaaaaac tttaacggca aactcatcct catcggactg
      301 atgggcgcgg gcaaaactac gctgggccgg caaatggcgc agcggctgga ttaccgtttt
      361 tacgacagcg atcacgaaat cgccgcagcg gcgggcgttc ccatccccac catatttgaa
      421 atggaaggcg aacagggatt ccgttcgcgc gaaaccgcca tactcaaaaa actgattgtc
      481 ctgccgcata tcgtcctgtc caccggcggc ggcgcggtgt taaaagaaga aaaccgcgcc
      541 cttatccgca aaagcggcac ggtcgtttat ctgcacgccc cgcccgaaac cctgctcgaa
      601 cgcacgcgct gcgacaacag ccgccctttg ctgcaagttg ccgatccttt ggcgaaattg
      661 cgcgaactct acgccgcacg cgaccccgtt taccgccaaa ccgccgactt taccgtagaa
      721 tcggcaaact gccgggaaac cgtgcaaacc ctgctcaaac gcttatcccg ataaaccggc
      781 atatgcgccg ctccccgaaa accaaaccgc accccgcccg gcgggccggc ggttcaaact
      841 ttaaggaaca acaatgaaaa cactgaccgt acacacgcct tcgcacagct accccatctt
      901 tatcggcaac gggctgctgc cgcaggcggg aagcctgctc aaaccgcatt tgggcaaacg
      961 cgccgccatc atcaccaacg aaaccgtcgc cccgctctac ctcggcacgc ttcagacggc
     1021 attggatgcg gcaggcgtat cccatttcag catcatcctg cccgacggcg aggcgcacaa
     1081 aaactggcag acgctcaacc tcatctttga cgggctgatg caaaaccgcg ccgaacgtaa
     1141 aaccacgctg attgcgctgg gcggcggcgt gatcggcgac atggtcggct ttgccgccgc
     1201 cacctaccag cgcggcgcgc cgttcatcca aataccgacc acgctgttga gtcaggtcga
     1261 ctcatcggtg ggcggaaaaa ccgccatcaa ccacccgctc ggcaaaaata tgatcggcgc
     1321 gttttaccag ccgcaggcgg tgcttgcaga tttggacacg ctgcacaccc tgcccgcccg
     1381 cgaactgtcc gccggtatgg cggaagtcat caaatacggc gcgctcggcg acatcggctt
     1441 ttttgaatgg ctggaacagc atatgcccga actgatggca ctcgaacgcg cgcccctcac
     1501 ccaagccgtg taccgctgct gccaaatgaa ggcagacatc gtcgcccaag acgaaaccga
     1561 acagggcata cgcgcatggc tcaacctcgg acacaccttc ggacacgccg tcgaagcgga
     1621 aatgggttac ggcgtatggc tgcacggcga ggccgttgcc gccggctgcg tgttggcggc
     1681 gcgtttgtcc gaacaactgg gcaaaacctc cgccgcagat accgcgcggc tcgccgccct
     1741 gctcgaagcc gccggactgc cgtccgcacc gcccgtgttt gcctttgaaa aatggctggc
     1801 acatatgagc cacgacaaaa aagtcagcgg cggcatcatg cgctttatcg gtctgaaccg
     1861 gctgggcgaa gccgtcatca ccgaaattac cgacacggac atcctccggc gcaccctgca
     1921 accgtatctc tgatttcctc tgccgatgtg ttgccgcgcg ggtttgacgc acgatgatga
     1981 ctttccatca tcattctccg cgaaagcggg catccggtta ttccatattg cagggattta
     2041 tccggaataa cgacaacctg tccgccgtca tttccgcgca gatggaaatt tggaaattca
     2101 aaatcacagg aatttatccg gaattatagc ggattaacaa aaatcaggac aaggcggcga
     2161 agccgcaggc agtacggatg gtacggaacc ggttcgcccg gtgcttgggc gccttaggga
     2221 accgttccct ttgagccggg gcg
//
LOCUS       DAWTHD010000164         2235 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWTHD010000164 DAWTHD010000000
VERSION     DAWTHD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.164"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..311)
                     /locus_tag="WM704_002081"
     CDS             complement(<1..311)
                     /locus_tag="WM704_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341416.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     K"
     gene            complement(369..734)
                     /locus_tag="WM704_002082"
     CDS             complement(369..734)
                     /locus_tag="WM704_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341417.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(716..1255)
                     /locus_tag="WM704_002083"
     CDS             complement(716..1255)
                     /locus_tag="WM704_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341418.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            complement(1298..2167)
                     /locus_tag="WM704_002084"
     CDS             complement(1298..2167)
                     /locus_tag="WM704_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFA9341419.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQ
                     SSSELKLGVQHTF"
BASE COUNT          579 a          490 c          487 g          679 t
ORIGIN      
        1 attttattat tttttattga ttttaagaca atttctgaaa aattctcttc gctttctccc
       61 ttttgtagaa gcacatcaga aaaaataaaa ctttcccgat taaattcata aatatgtttc
      121 aaccattcac ctcctctttc tgtaaggcaa gattcagttt cattcttcct tattgtataa
      181 atatttcctt cacaaaatct gaaataaatc cataaatcca tcttatccat aattaaagaa
      241 aaagtttcac ctcgagattt tgtcaacaat tcgcaaggtt gcgatgttgc aatcaaatag
      301 ccgaaagaca ttttttacct catacatggt cgaaatcagt ttctgttagt tcagaatcca
      361 ttttttcgtc aacaactgaa tccgcatttt tgaattaacg ttttcatcag ctgccgttta
      421 tctaaaccgg caggttcagt ttcagaataa gccttatatg aagactgtaa gcatttcaga
      481 aaaagatcat cagaaggcat atctgccgaa tcaaatacaa ctgttttgat tttggtactt
      541 acccaaaacc ctttttgctc tttttctact atacggaaat tcagaatatt tccaaccgaa
      601 tcaaaagcac ggtaaacatc atccatcaaa tcctgcggct ctattttctt ttccaattcc
      661 gacaatcctt gaaatatatc caaagacaca tcttcaaata gaaaaaaagg aggagttaga
      721 agcggttttt ccatgatctg tccgtagatt ttgattccca agggcgatga cgaccaattc
      781 cctgtccagg caaagtcttg cccgtattat ccgtaactcg acgatgataa tggggaaatt
      841 ttccaatagg atgacctgtt ctattaccga aaggggctat ccgcatatta ttgccgattt
      901 taatctcacg tccatattta gcaaaggaaa caacctttcc tgcagcacct acatcaccag
      961 gaattgcgcc taatccgcca gcaacatctc taacagaagc tggtctgcct gtcgttgcat
     1021 aactaaaacc atgctgtgtc cacataccaa tggcagcacc acccaagata gccaatggaa
     1081 gaaaagcccc ttcagtctcc ttcatctcct tctgagaaag ctccgccaac tgcatcggcg
     1141 catctgcccg cgtgtggaac acttggtctt caaatgcctg attgtccaat ccgtttgcca
     1201 ttgcgggggc gatcatagac agcatcatta cggctgcggt gatttgtttt ttcataataa
     1261 ctcctttgga ttacaaggtt ggaaaatcaa aatctgctta aaatgtatgc tgtacgccca
     1321 atttcagttc ggaactgctt tgccctgaaa cgttgaaacg tgcggatgcg tttaaagccg
     1381 cggttttggt gaaaccgaaa cctgcgccga aatgggcgta ggtggatgtg tttcttgcgg
     1441 attctttttt gccgtctatg cggtcgggct gtttgcccag ccattggatg cctccggtca
     1501 ggctgattct gtcgttggcg gcaaatgaga tgttgggatt cagcatccag taattgcctg
     1561 ctttgtattt gacgtcgtct gaaagggttt tgctgccgtt gatgcggtag gcggcggtga
     1621 gggaaaggac aatcggatct atggctttgt aggtggtggc gccgatgagc cacgattttc
     1681 ccgacgaggc tttgttgcgc gatttttcgt aaaccgtgct ttcgaggaaa gcgatgagtg
     1741 cgggattttt gccgtcttta aggaaggtgt ggctgatgcc ggcggatatg tcggacatcc
     1801 gtttgttgcg ggttttgccg ttgccgtcga gtttgcgttc ttcgtgccac agatagctgc
     1861 cgctgccgta aatgtcggta ttgccggtca gtccgtagcg caaaccgagc gtgccggcga
     1921 gcatatcggt attgctgccg ttttcttgaa tttcggtcgg aatggggata aacgaggttg
     1981 cgccggtttg aatgtaaacc ggtgcggcaa gtgcggcacg gctgttttcg ctattcagat
     2041 aggtaaggga agtttccagt ttccattttc ccttgtcggt cattatgtct tcaatcgtca
     2101 agggcaggtc ggcataagcg gataaaggca ggatggcggg caaggcgggc aaaaagatac
     2161 gtttcatatt tcctcttatt gatattaatt cttatttaat aatagtaatg aatatccgat
     2221 aattctgtct aaaca
//
LOCUS       DAWTHD010000165         2148 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWTHD010000165 DAWTHD010000000
VERSION     DAWTHD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.165"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            23..680
                     /locus_tag="WM704_002085"
                     /pseudo
     CDS             23..680
                     /locus_tag="WM704_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(945..1496)
                     /locus_tag="WM704_002086"
     CDS             complement(945..1496)
                     /locus_tag="WM704_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341420.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
     gene            complement(1501..1605)
                     /locus_tag="WM704_002087"
                     /pseudo
     CDS             complement(1501..1605)
                     /locus_tag="WM704_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1655..2148)
                     /locus_tag="WM704_002088"
                     /pseudo
     CDS             complement(1655..2148)
                     /locus_tag="WM704_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          628 a          566 c          415 g          539 t
ORIGIN      
        1 gtttattccg cgtatacata ttatgataaa aaatctttat aaattgataa attatgcggc
       61 tttgattggt ttcgccccaa atatagtttt tgcttggcct atgtttgttg acccccgctt
      121 cttaccaaaa catccatgta cggagttgga aagcacggcg agattccggc atagtaaaac
      181 aagatttgga tttttcttgc ggcgcggctt cgattgccac gttactgaat aatttttatg
      241 gcagacatta ttctgaagcg gaaatcttag acaaaatgga taaaacccaa atgcgtgctt
      301 cttttgatga tatgcaacgc ataatgcccg aactgggttt tgaagcacaa ggttatgctt
      361 tgccatttga acagttggta caactaaaaa ttcctgtaat tgtgtattta aaataccgta
      421 aaaacaacca tttttcggta ttgaacggta ttaacggaga aactgttttg ctggctgatc
      481 catcgctggg gcacgtctca acgagcaaat cacaattctt gagcgcatgg aaaacacgtg
      541 atggcgaaat ggaaggaaaa attttagcca tcgtgccaaa aaatactgat tttgttagaa
      601 atcagatgtt ttttaataaa aatcccgttc gtcaaacacg ttttacggta gaacaaatcc
      661 aaatgcggca aaaacgataa taaaccgatt tttgcccatt tattttatat agtaatcagg
      721 acaaggcggc gggccgcaga cagtacaaat agtacggaac cgattcactt ggtgcttcag
      781 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta
      841 atccactata aaagcaaaga aaccgccatt agcactacac ccccccaagc tgcggctgcc
      901 aaacacatcg gctgcttctc gacgcacccg ctgaaaaatg cgccctatcc gcccgccttg
      961 accgcacccc ctgcctttgc caccctgccc gatgccgtct gaagcctgaa ccgccctacc
     1021 agcccgatga tgcacgacac cgccggaaag ggcatacaca aagccgtcag cacataacgc
     1081 gcattccccg tattgaccac gtccgcgccc ttcagcagca gcggcagcgc gttcgcccac
     1141 tcccacagcg aaggcgcgct gaacgccaat accgccacca gccccaacat ccggaacacc
     1201 ccgatttcca agacctgccg ccgccgcacc gaacggttga gcaaaaacac aaccgccaac
     1261 ccgaaacaga ccaccgccgc cataccgccg ttggcgacaa tctgcaaact gttgaacgca
     1321 atatcgggcg taaccgccaa cacgccttcc gaatccggca cggcacgctg caaatccaac
     1381 ccctgcaaca cattcaacac aaaaccgtag atcaaatcaa aaaacaccac gcctatcggc
     1441 aaagcactca aaacccgcca catcttccta tctgaccaac cgttcaaaac cttcatttta
     1501 tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta tttgtactgt
     1561 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctatacccga ccacgcaagc
     1621 cgccgaacaa aaaataaggg gctgtcctag ataactagga taaactcgat tttactaatt
     1681 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgccatt cgcactcctt
     1741 taaatacagc tcaaaatgcg ctttgggaat gccgttaaac ttgcgtaaat gacgttttgc
     1801 ctgattccaa aagttctcaa ttccattaat atggttttgt cgttcggcaa aatgtgtgct
     1861 gtgattgata cgaaaacgaa gtttcagcga agctaaaatg gctaaattcg ctcacatcta
     1921 atacatcata gctacgataa caatccgtat aaacaatact gtcaggtttc acttgttcac
     1981 ggataatagg aaataaagta gcggtttgag tattcggtac tgtaaccgta taaaccttac
     2041 catttcgctt caaaagaccg aatacggcga ctttaccggc agcaccgcga ccgcgtttgc
     2101 ctttgcgttg tccgccaaaa taactttcat ctgcttctac ttcgccat
//
LOCUS       DAWTHD010000166         2007 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWTHD010000166 DAWTHD010000000
VERSION     DAWTHD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.166"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..619
                     /locus_tag="WM704_002089"
     CDS             <1..619
                     /locus_tag="WM704_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9341421.1"
                     /translation="SDYFRNIRTHSIHPRVSVGYDFGGWRIAADYARYRKWNNNKYSV
                     DIKELGRNDKTSSGKKNHLNIQTQKTERQENGTFHAVSSLGLSTIYDFDTGSRFKPYI
                     GMRVAYGHVRHQVHSMEKETTAVTTYPSNGGTPSVTTEAPTKKPAHHESRSISSLGFG
                     AVAGVGIDITPKLTLDAGYRYHNWGRLENTRFKTHEASLGMRYRF"
     gene            633..827
                     /locus_tag="WM704_002090"
     CDS             633..827
                     /locus_tag="WM704_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341422.1"
                     /translation="MPSEPSDGIFDALAVYGRGAGAVKYPNRHSRQYRNLEIRHSRAG
                     GNPDLSGFAQIGRRRDGVAD"
     gene            811..987
                     /locus_tag="WM704_002091"
     CDS             811..987
                     /locus_tag="WM704_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341423.1"
                     /translation="MVLQINDVDTNSGEFAIYTAQDASVKLDLLKMELKHCKEMLEQK
                     DKEIELLRKLTETV"
     gene            1076..1645
                     /gene="rsmD"
                     /locus_tag="WM704_002092"
     CDS             1076..1645
                     /gene="rsmD"
                     /locus_tag="WM704_002092"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFA9341424.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1768..>2007
                     /locus_tag="WM704_002093"
     CDS             1768..>2007
                     /locus_tag="WM704_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFA9341425.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKII"
BASE COUNT          555 a          505 c          516 g          431 t
ORIGIN      
        1 aagcgattat ttcagaaaca tccgtacgca ttccatccac cccagggtgt cggtcggcta
       61 cgacttcggc ggctggagga tagcggcaga ttatgcccgt tacaggaaat ggaacaacaa
      121 taaatattcc gtcgacataa aagagttggg aagaaacgat aagacctctt ctggcaaaaa
      181 aaaccatctt aacatacaaa cccaaaagac ggaacgtcag gaaaacggca cattccacgc
      241 cgtttcttct ctcggcttgt caaccattta cgatttcgat accggttccc gcttcaaacc
      301 ctatatcggc atgcgcgtcg cctacggaca cgtcagacat caggttcatt caatggaaaa
      361 agaaaccacg gctgttacca cttacccaag caacggtggt acgccaagtg ttaccacaga
      421 ggctccgacc aaaaaacctg cccatcacga aagccgcagc atcagcagct tgggcttcgg
      481 cgcagtggca ggcgtaggca tcgacatcac gcccaagctg accctggacg ccggctaccg
      541 ctaccacaac tggggacgct tggaaaacac ccgcttcaaa acccacgaag cctcattggg
      601 catgcgctac cgcttctgat tccccgatac cgatgccgtc tgaaccttca gacggcattt
      661 ttgacgcgct cgccgtttac gggcgcgggg cgggcgcagt aaaatacccg aaccgtcatt
      721 cccgacaata ccgtaatctc gaaattcgtc attcccgcgc aggcgggaat ccggatttgt
      781 cgggattcgc tcaaatcggg cggcggcggg atggtgttgc agattaacga tgtggatacc
      841 aacagcgggg aatttgcaat ctataccgct caggatgcat cggtaaagct ggatttgtta
      901 aaaatggaat taaaacactg taaagaaatg ttggaacaaa aagacaaaga aatcgagttg
      961 ctccgcaagc tgaccgaaac cgtttaaaca gatatgccgt ctgaaaaaag ttttcaggcg
     1021 gcatattctt cgacaggtct tgtataatac cgtttgaact ttcaggcttt tgattatggc
     1081 ggcaggcaaa cataccaaac acagcaaccg ggtacgcatt atcggcgggc aatgccgggg
     1141 caggaaattg agttttgcat ccgccgacgg actgcgccca acccccgaca gcgtgcgtga
     1201 aaagctgttt aactggctgg gacaggattt gacgggaaaa acggttttgg atctcttcgg
     1261 aggcagcggc gcactcggca tggaggcggc atcgcgtaat gcaaaacggg tcgtgattgc
     1321 agacaacaac cgtcaaacgg tgcaaaccct tgagaaaaac agccgcgaac tgggtttggg
     1381 gcaggtgcaa accgtctgtt cggacggcat tgcctatttg gcaaacctaa aagagaaatt
     1441 cgatgtcgtc ttccttgatc cgccgtttgc gtggcaaagt tgggaaagtc ttttcaatgt
     1501 attgggcaca cgcctgaatg acggcgcata tgtttatatc gaggcaggca ggcagccgga
     1561 caaacctgat tggctgacgg gatataggga ggggaagtcg gggcagggta catttgaatt
     1621 aagggttttc caagtggctg aataatatgc gctttgataa tcatttccgg gttgtaaaca
     1681 ttcgtttgca accgtccggt tcaaaaaaac cttgtgctat aatccgcgcc cgcccggttt
     1741 tgataattta gtggaaaagg aaaagaaatg tcgcttttta ttaccgacga gtgcatcaac
     1801 tgcgacgtat gcgaacccga atgccccaat gatgccattt cccaaggcga ggaaatttac
     1861 gaaatcaacc ccaacctctg cacgcagtgc gtcggacact acgatgagcc gcagtgccag
     1921 caggtttgcc cggtggactg catcctgatt gacgaagaac atcccgaaac ccatgacgag
     1981 ttgatggcga aatacgaaaa gattatc
//
LOCUS       DAWTHD010000167         2004 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWTHD010000167 DAWTHD010000000
VERSION     DAWTHD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.167"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            34..1707
                     /gene="recN"
                     /locus_tag="WM704_002094"
     CDS             34..1707
                     /gene="recN"
                     /locus_tag="WM704_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFA9341426.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            1818..1894
                     /locus_tag="WM704_002095"
     tRNA            1818..1894
                     /locus_tag="WM704_002095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1852..1854,aa:Arg,seq:acg)
     gene            1912..1986
                     /locus_tag="WM704_002096"
     tRNA            1912..1986
                     /locus_tag="WM704_002096"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1945..1947,aa:Glu,seq:ttc)
BASE COUNT          532 a          494 c          543 g          435 t
ORIGIN      
        1 ttttgtcggt agaattgcta aggaatcctc acaatgcttc taacactttc tttgcgtgat
       61 tttgtcattg tcgaaaatct gaatctggat tttcaaagcg gttttaccgt tttgaccggc
      121 gagaccggcg cgggcaagtc cattactttg gatgcgattg gtctgctgtt gggcgataag
      181 gccgattaca gccaagtccg cagcggcgca aaagaagcgc agttgtcggc attgttcgat
      241 atttcccatt tgcctgcttt aaaagcagaa ttgcgtgaac aagggctgtt ggatgagggc
      301 ggggaagaac tcagtatccg ccgcattatc gatgccaaag gcaaaagccg cagttttatc
      361 aacaatcagg cggctacctt ggcgcaactc aaagccgtcg gcgggcagct tatcgacatc
      421 cacgggcaaa acgaccatca ttcgcttaat caggaagctg cccagcgcga attgttggac
      481 gcatttgtgg gcggcagggt gcaggcggaa accgtcaggc agctttatca aaactgggcc
      541 aatgcgaaaa aagccctcca agaggcgcag gaacacgccg atgccgtcat tatcgagcgg
      601 gagcgtctgg aatggcagtt taacgaactg aatcagttgg acattaaaca aggcgaatgg
      661 gaagctctca gccaaagcca cgacagcctt gcccattctg ccgaattgtt gcaggccgcc
      721 gaagaagtcg gaagcaagat tgacggcgac aacggcatcc aacgccatat ctatcaatgt
      781 caaaaactat tggccaatct gcaaaacatc gagccgcgct ttgccgagag cctgaatatg
      841 ttggcaagca tcgaggccga attgggcgaa atcagtgcca atatgcgcga tgtggcagga
      901 cacagtgaca tcaaccccaa tgaattggcg gcacaggaac agcgtatggg cgaactgatg
      961 gggatggcgc ggaaataccg gattgaaccc gaagagttgc ctgccaagtt ggcagaaatc
     1021 gaagaacgcc tgcaaagtct tcaagctgcc gctgatttgg acgcgctcga gcataatgtt
     1081 gcccacaatt ttgccgaata tcaggaagct gcccacatcc tttctgccat gcgccatcag
     1141 gcggcagggc gtttgagtag cgaaacgacc gagcatatgc aacaccttgc catgaaaggc
     1201 gcgcgtttcg acatcgtcct gttgccttcg tcgccgacgg cacacggttt ggagcaggtt
     1261 caatttcaag ttgccgctaa caaaggcaat ccgccccgcc cgctgaacaa agtcgcctcc
     1321 ggcggcgaat tggcgcgtat cagccttgcc ttacaggttg ttgccagcca atatacccaa
     1381 gttcccaccc tgatttttga tgaggtcgat accggtattg gagggggagt ggctgaaatg
     1441 gtcggcaagg cattacgtgc gttgggcaga aaacatcagg tgcttgccgt tacccatctt
     1501 ccgcaagtcg catcctgcgg agaaaaccac tggcgggtgc gcaagcacag cgagggagag
     1561 caaaccgtca gcgaaatcag tatattggat gaaatacaac ggatcgaaga ggttgcccgt
     1621 atgttgggcg gagaagttat taccgatacg acgcggcaac atgcggcaga attgctgcaa
     1681 cttgcgtcga aaaatagttt attttaaaat caatcagtta caaaaataac tgaaaataga
     1741 agtctaaaat aatagacagg actcagataa atccgtatta tcacgctttc ttaatcactt
     1801 gaacaagtga ttgtgccgca cccgtagctc agttggatag agtatctggc tacgaaccag
     1861 agggtcgggc gttcgaatcg ctccgggtgc gccagtaaga aaatacaata tgcgcccatc
     1921 gtctagcggt taggacatcg ccctttcacg gcggtaaccg gggttcgatt ccccgtgggc
     1981 gtgccaattc aaaatgcctc cgat
//
LOCUS       DAWTHD010000168         1914 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWTHD010000168 DAWTHD010000000
VERSION     DAWTHD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.168"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(54..608)
                     /locus_tag="WM704_002097"
     CDS             complement(54..608)
                     /locus_tag="WM704_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341427.1"
                     /translation="MNTMNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFE
                     LLIDEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSG
                     RMSRKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMK
                     EIFDEILRIENLDRKNLPSNIMVK"
     gene            complement(695..1798)
                     /locus_tag="WM704_002098"
     CDS             complement(695..1798)
                     /locus_tag="WM704_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1845 family protein"
                     /protein_id="HFA9341428.1"
                     /translation="MQQQCFMKLFLITYKDLKMEFAKQRYIIKPGAIHVAQQFDLKVT
                     TKQSPFSDGYDMARAALALGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRK
                     HHLVWGEYEILSSENDRFKNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGI
                     RYGLTLSNELFGMAMSDNPYAQSRLLQIEKQMSEIEKFFETVKKQVHAQIDSLAAAGM
                     KITLIQNNNPVHIRLNVLRAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLY
                     NGGRMMRKLLNDLYIAVMETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWV
                     YESQPSLVYIQRKMNQEDLNKLVDIVRDNGLSG"
BASE COUNT          596 a          444 c          276 g          598 t
ORIGIN      
        1 aaaaaaacca tcacaaaagt gatggcaaat gatttttaag gctattaaat atcttactta
       61 accatgatat tactaggaag atttttgcga tccaaatttt caattcttaa aatttcatca
      121 aaaatttctt tcatcgaaaa caattctgcc aagtctccgg caatctgcct acctcggcaa
      181 aaatactgtc tgcaatcaaa ataaaccttc atatgtaata accgttcatc aaggctggca
      241 ttcgtatcat ccaacgccgt actaatatcg gcccatttaa aaaacttctt tctcgacata
      301 cggccactgg aagaaacaac atttttgaaa aaaatcatat tcggaatata gtattttttt
      361 aaatccaaac cagccaaact tttattactc aatttcagat gttctcttac tataggatat
      421 atgagattaa ggccctgaca tgcttcatga aacgactgag cttcatcaat taatagctcg
      481 aaatcaacca atttcaattt aatttctttt tctaaatcct caatccccct tacccttaaa
      541 agccttttgt attcccaaaa ttttaagtat tttttcatac ctgatatttg agtattcata
      601 gtattcatat cacatcataa aaaaatccat acaaacaagg gccaatcaat accatcttaa
      661 aactcgattg taaccccacg ttccaatttc acaactagcc cgataatcca ttgtcacgaa
      721 caatatcaac caacttattg agatcctcct gattcatttt tctctggata taaaccaaag
      781 agggttgtga ttcatacacc caaacagaca atggaattct aggcagaatc tcttgctcaa
      841 cagcagattt caacttcgac aactgctctg ggtcaagcag actgtcacgt cgaatgctct
      901 tgatagccct ggtctccatt accgcaatat ataaatcatt cagtagcttt ctcatcatcc
      961 gaccgccatt atacaacgtg tcattacatt gtttattagc catcatgcct ttgatattaa
     1021 gtgtcttaac ggcacataca aaactatcgt aatcccgcaa caatttaacc agtttgaaac
     1081 cataagcccg caatacattc aatctgatat gaacagggtt attgttctga atcaaggtta
     1141 tcttcatacc tgcagcggcc aaactatcaa tctgagcatg aacctgtttt ttcacagtct
     1201 caaaaaattt ctcaatttcg gacatctgtt tttcaatctg caataaccga ctttgggcat
     1261 aaggattatc ggacatagcc ataccaaaca actcgttact gagtgtcaga ccatatctga
     1321 ttcccggcca acgtgggccg ttcttcttgt tgttattgcc ctcccacata cggatacctt
     1381 gcttggtatg cataaaaaaa ccatcatcac cggaagaggt caggctgcca atatttttaa
     1441 accggtcgtt ctcagaagaa agaatttcat actcccccca aaccaagtga tgctttcgac
     1501 ctgtctcctt ttgtagatgt tgcttgagca gcaattcatg tgctttaaac tcttcccact
     1561 gttggaactg ttcgtcatcc tcagtcaact tttgattgac cttatccccc aaagccaaag
     1621 cagcacgagc catatcgtaa ccgtcactga aaggtgactg ttttgttgtg actttgagat
     1681 caaattgctg tgcaacatgg atcgcaccag gtttgatgat atatctttgt tttgcaaatt
     1741 ccatttttaa atccttataa gttattaaaa agagcttcat aaagcactgt tgttgcatat
     1801 cagaggctaa ttagtcatgt acaccatcat atctgtcctt tgccgatgac tgtaacgccc
     1861 cgttacatcc gcttcgaagt aatagattta ttcgtctttc aaaaattcat caaa
//
LOCUS       DAWTHD010000169         1903 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWTHD010000169 DAWTHD010000000
VERSION     DAWTHD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.169"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(270..370)
                     /locus_tag="WM704_002099"
                     /pseudo
     CDS             complement(270..370)
                     /locus_tag="WM704_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            502..1425
                     /locus_tag="WM704_002100"
     CDS             502..1425
                     /locus_tag="WM704_002100"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFA9341429.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
     gene            1548..>1903
                     /locus_tag="WM704_002101"
     CDS             1548..>1903
                     /locus_tag="WM704_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9341430.1"
                     /translation="MPARAQVSEAILLAEGQKSAVTEYYLNHGIWPKDNTSAGVASPA
                     SNIKGKYVQKVEVNNGVVTATMASSNVNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRT
                     GDNDDTVADAKDGKEI"
BASE COUNT          479 a          534 c          467 g          423 t
ORIGIN      
        1 ccgccccccc atttgccgaa caagcggtcc ggactcccgc ccgcgcggga atctaggccg
       61 gtcggtgcgg aaacttatcg ggaaaaaagg tttcttgaga ttttacgtcc tggattccca
      121 cttttgtggg aatgacgtgg tgcaggtttc cgtacggatg gattcgtcat tcccgcgcag
      181 gcgggaatcc ggaccttaga acaacagcaa tattcaaaga ttatctgaaa gtccgggatt
      241 ctagattccc gcccgcgcgg gaatgacggg atgtatagtg aattaaattt aaaccagtac
      301 ggcgttgcct cgccttttcg tactatccgt actgcctgcg gcccgccgcc ttgtcctgat
      361 tttcgttaat ccgccataca ttttcatgcg gcttacgtgt tggaaacgga tacattggca
      421 aatactattg aaaaacatag cctgtttttc cggttttatc gtattattcc gaaacttacc
      481 ccattgataa ggaagcggtt tatgctgcaa agaactttgg cgaaatccat cggcgttacc
      541 ggagtcgggc tgcattcggg cgaacgcgtc gcgctgaccc tgcaccctgc gcctgaaaac
      601 agcgggattt ccttccgccg caccgatttg gacggcgaga tgggcgaaca aatcaagctg
      661 aacccttatt tgatcaacga tacccgcctt tcatccacca tcgttaccga caaaggcctg
      721 cgcgtcggca ccatcgaaca catcatgtcc gcactgtccg cctacggcat cgacaatgcg
      781 ctgattgagt tgaacgcgcc cgaaatcccg attatggacg gctccagcct gccgtttatt
      841 tacctcctgc aagatgcggg cgttgtagac caaaaggcgc aaaagcgttt tttgaaaatc
      901 ctcaaacccg tcgaaatcaa agaagcgggc aaatgggttc gatttacgcc gtatgacggt
      961 tttaaggtaa cgctgaccat cgaattcgac catcctgttt tcaaccgcag cccgcctacg
     1021 tttgaaattg atttcgccgg caaatcctac atcggcgaaa tcgcgcgcgc gcgcactttc
     1081 ggctttatgc acgaagtgga aatgatgcgc gcccacaatc tcggcttagg cggcaatttg
     1141 aacaacgcca tcgtgattgg cgacacggat gtcctgaatc ctgaaggctt gcgctatccc
     1201 gatgagtttg tgcgccacaa aatcctcgac gccatcggcg atttgtatat cgtcggacat
     1261 ccgattgtcg gcgcgtttga aggctacaaa tcgggacacg ccgtcaacaa cgcccttttg
     1321 cgcgcagttt tggcagacga aacggcttac gaatgggtgg aatttgccga cagcgatgat
     1381 ttgcccgacg catttcacga gctgaacatc agaaattgcg gataacgccc ccttttccaa
     1441 acaaatgccg tcaaacagat gccgtcattc ccgcgaaagc gggaatccgg aacgcaaaat
     1501 ctcaagaaac cgttttaccc gataagtttc cgtgccgaca gacccggatt cccgcccgcg
     1561 cgcaagtttc cgaagccatc cttttggccg aaggtcaaaa atcagccgtc accgagtatt
     1621 acctgaatca cggcatatgg ccgaaagaca acacttctgc cggcgtggca tcccccgcct
     1681 ccaacatcaa aggcaaatat gttcagaaag ttgaagtcaa caacggcgtc gttaccgcca
     1741 caatggcttc aagcaacgta aacaaagaaa tccaaggcaa aaaactctcc ctgtgggcca
     1801 agcgtcaaga cggttcggta aaatggttct gcggacagcc ggttacgcgc accggcgaca
     1861 acgacgacac cgttgccgac gccaaagacg gcaaagaaat cga
//
LOCUS       DAWTHD010000170         1900 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWTHD010000170 DAWTHD010000000
VERSION     DAWTHD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.170"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            102..1112
                     /gene="trpS"
                     /locus_tag="WM704_002102"
     CDS             102..1112
                     /gene="trpS"
                     /locus_tag="WM704_002102"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFA9341431.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            1193..1738
                     /locus_tag="WM704_002103"
     CDS             1193..1738
                     /locus_tag="WM704_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341432.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
BASE COUNT          506 a          529 c          483 g          382 t
ORIGIN      
        1 atatggaaaa gtctgttgtc ggctggatgt atgtggcaaa tcgggtataa tcggcgcatc
       61 ttttcccttt cagacggcat tgctgccgta aggacatttt tatgagcaaa aaacgagttc
      121 tgaccggcgt aaccaccacc ggcaccccgc atctgggcaa ctacgtcggc gccatccgcc
      181 ccgccgtccg cgcggcgcaa aaccccgata ccgaatcctt cctcttcctc gccgattacc
      241 acggcatcat caaatgccac gagcaggaga tgattcacca atccacccaa gccgttgccg
      301 ccacttggct tgcctgcgga ctcgaccccg agcgcaccac cttctaccgc caaagcgaca
      361 ttcccgaagt gatggagttg aactggattc tgacctgcat caccgccaag ggtttgatga
      421 accgcgccca cgcctacaaa gccgccgttc aagccaatgc ggaaaacggg caggaagacc
      481 ccgatttcgg tgtggaaatg ggtttgttca gttatccgat tctgatgact gccgacattt
      541 tgatgttcaa cgccaacgaa gtccccgtcg ggcgcgacca aatccaacac gtcgaaatgg
      601 cgcgcgacat tgccggccgc ttcaaccacc gtttccaaga actcttcacc ctgcccgaag
      661 tgaaaatcga cgaaaacgtc gaactcttgg tcggtttgga cggacgcaaa atgtccaaat
      721 cctacggcaa taccattccg ctttgggaaa acgacaaaaa aacccaaaaa tcggtcaaca
      781 aaatcattac caacatgaaa gagccgggcg agccgaaaca gcccgacgaa agccctttgt
      841 ttgaaatcta taaagccttc tccacgccgt ctgaaacggc ggaatttacg caaatgcttg
      901 ccgacggctt ggcttggggc gaggcgaaaa aactttcggc ggcgaaaatc aacgccgaac
      961 tggcagaact gcgcgaacgc tacaacgcat tgacctctaa tccttcgcaa atcgaagaga
     1021 ttttgcaggc aggcgcgcaa aaagcgcgca aagaagcgcg cgaattgttg gataaagtac
     1081 gcgatgcggt cggcatccgc ccgttgaaat aaacccaatg ccgtccgaac ctccgcctgc
     1141 cacgtttcag acggcatttc agacggcatt ttgaaaccat caggagtgcg gaatgctgaa
     1201 aatacctttt gccgtgttgg gcggctgcct gctgcttgcc gcctgcggca aatccgaaaa
     1261 tacggcggaa cagccgcaaa atgcggcaca aagcgcgccg aaaccggttt tcaaagtcaa
     1321 atacatcgac aatacggcga ttgccggttt ggctttggga caaagtagcg aaggcaaaac
     1381 caacgacggc aaaaaacaaa tcagttatcc gattaaaggc ttgccggaac aaaacgccgt
     1441 ccggctgacc ggaaagcatc ccaacgactt ggaagccgtc gtcggcaaat gtatggaaac
     1501 cgacggaaag gacgcgcctt cgggctgggc ggaaaacggc gtgtgccata ccttgtttgc
     1561 caaactggtg ggcaatatcg ccgaagacgg cggcaaactg acggattacc tgatttcgca
     1621 ttccgccctg caaccctatc aggcaggcaa aagcggctat gccgccgtgc agaacggacg
     1681 ctatgtgctg gaaatcgaca gtgagggggc gttttatttc cgccgccgcc attattgagt
     1741 tattcggaca tcacggaata tatttggatt tttcaaacct tgcggaaaaa gtccgcgccg
     1801 ccgggaaact caaaaggagg ggatgtgtgt tacaattttc cgaactgttt tcataaaata
     1861 gttttcggac gtgtttcaat atggcattga tgccgccttg
//
LOCUS       DAWTHD010000171         1873 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWTHD010000171 DAWTHD010000000
VERSION     DAWTHD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.171"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            71..841
                     /locus_tag="WM704_002104"
                     /pseudo
     CDS             71..841
                     /locus_tag="WM704_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            868..1560
                     /locus_tag="WM704_002105"
     CDS             868..1560
                     /locus_tag="WM704_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9341433.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            1752..>1873
                     /locus_tag="WM704_002106"
     CDS             1752..>1873
                     /locus_tag="WM704_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HFA9341434.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIY"
BASE COUNT          411 a          397 c          562 g          503 t
ORIGIN      
        1 ataacccaac gaaaatacta aaattccaag ctaaggctat cctttccaaa ccatccccat
       61 caaccaaacc atgattaaaa ccgacaaaat ccgcaaaccg cagcctgcgc tgttttacat
      121 catcgactac ctttggagcg gctttgccgg tctgagcgtg gcgatggtgg tggtggcgtt
      181 gtgggcgtgg ggcagcgccg tgttcggcga gtttatgctg cctgcgccgg tagaggtgtt
      241 tcaaaaatct ttggatttat tgaaacattt tcaggaaaat gaaatcggga tttcgctgtg
      301 gcggtcggta gtgggtattt cggttgcttt gatagcagga ttggcggcgg ggctggtggc
      361 gggcagtttt aagacggcga tggcgttgct taagcctgtg attacgattt tgttagcaat
      421 gccgccgatt atttgggtgg tgatggcgtt gttttggttc gggttcggca atccgagcgt
      481 gttgtttacc atcattgtgt tggttgcgcc gctgacgttt gcgagtgcgg cggtcggaat
      541 ggcgagcgtg aacaagcagc atgaggagtt gtttgacgct tataaattag gccgtctgaa
      601 aaaaatccgc tatctgtata tcccgcatct gacgggctat gtgatttcca gcgtcggcgt
      661 ggcggtggcg atgggggtga aggcggtgat catggcggaa ctcttaggcg cgagcaaagg
      721 cgtgggcgcg cggattgcgg acgcgagggc aatgctgtag acttcgacgg taatggctta
      781 tgtggtgttg gtcatggtat ttgtgtcgct gtttgaatac ctgattacca agcctttgta
      841 aattttgttt atgccgtgga ggagatgatg ctctgtcttg aaaacgtgcg ttttgaaatc
      901 ctccgcgacc ccatcgtgcg cgatttcagt ttgaacctgc aacagggcga agtgaaagcc
      961 ttgttcgggc cgagcggctg tggcaagacg acggttttac ggctgattgc gggcttggaa
     1021 acgccgaaat cgggcacgat acgtaatact ttccacaaaa cgggttttct gtttcaggaa
     1081 aaccgcctgc cggaaaacct gaccgcgatg cagaatatcg cgatttttat ggacaaccca
     1141 gacgaaggcg aaatcgtcgc gctggcggcg aaagtcgggc tgactgcggg cgatttgaac
     1201 aaatatccga ccgaattgtc cggcggcatg gcgaaacggg tggcgttttt gcgcctgctg
     1261 ctgtgcggct gcgacctagc cttgctggac gaaccgtttg tcggtttgga ccgcgatttg
     1321 cgcgatattt tggttgctat gctggtggaa aaaatcgagc ggcagggcat ggcgtgtata
     1381 ctggtaacgc acgaccgctt cgaagccgcg cgcctgagcc atgaaattat gctgctttcc
     1441 gctaagggca tgaacgtgca aaacgtgata accctgccca cgccgctgtc cgaacgcgat
     1501 tcggcttttg aagaagtcgt ggtggcaagg gagtttcagg ggattcatta ttatgagtga
     1561 tgaggggatt tgagtgtctg acaaaccttg cggttcgttg ccctctccct aaccctctcc
     1621 cacggggaga gggttagcaa gcctcaggct tacctcttta gcgaaagata cgaatctgtt
     1681 atccgaacgc gattcggttt ttgaaaaagc cgtggtgcca agggggcagg ggattcatta
     1741 tgaggtgctt tatgttttcg actgtgatta ctgctgctgt tttatatatt gctacagcag
     1801 tagatttgtt ggtaatacta ttaatatttt ttgctagagc aaatactaga aaagaatatc
     1861 gagatattta tat
//
LOCUS       DAWTHD010000172         1784 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWTHD010000172 DAWTHD010000000
VERSION     DAWTHD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.172"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            189..968
                     /locus_tag="WM704_002107"
     CDS             189..968
                     /locus_tag="WM704_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFA9341435.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            1177..>1784
                     /locus_tag="WM704_002108"
     CDS             1177..>1784
                     /locus_tag="WM704_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HFA9341436.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTS"
BASE COUNT          520 a          588 c          365 g          311 t
ORIGIN      
        1 gccgaaactt ttgtaaaact gtccatactc attaccgcct agtcggtaat gcatttttta
       61 gtagtatttg aaaaagtatg atgataaaaa cggttaagcc taactttttc ataaaatcca
      121 gcagagtaag gcagaaaaaa acacccaaat tggctataat cccgacaaac acactcaagg
      181 acaacaacat ggcagcctcg cccgaagcaa aattcaccga agaaaagatt ttgtgggtca
      241 aacaccacac gccgaaactc atcactttcg ccatcagccg tcccgaatcc taccgtttta
      301 aagccggaca gttctcccga ctcggtttct acgaaggtaa aggctttatt tggcgtgcct
      361 attccgtcgt ttccgcagaa tatgccgaca cgctcgaata ttttgccgta ctcatccaag
      421 acggccccat gtcggccctt ttcgccaaaa tgcaacaggg cgacaccatc ctgctcgata
      481 aaaatgccac tggctttctc ctgcccgaac gcttccccga cggcaaggat ttggtgatgc
      541 tctgtaccgg ctcgggcatc gcccccttcc tttccattct cgaacaaccc gaaatccggc
      601 agcgtttcga taccgtcaac ctgatacatt ccgtatcttt tcccgaagaa ttgattttca
      661 acgaccggct ggccgcattg tccgaacatc ccctggtagg cgaatacgga cactcgttcc
      721 gtttcgtccc tgttaccacc cgtgccgcca acccctcggg cttaagcgga aaacgcattc
      781 cggaactctt aaaaaacaac agcatcgaac aggcgctgca taccaagctt accccggaat
      841 ccacacggtt tatgatttgc ggtaacccgg aaatggtcaa agacactttc caaacgctgc
      901 tcgatatggg ttacgccatg caccgcaacc gcattcccgg tcaaatcatg atggaaaacg
      961 gcttctaaaa acgaacgctc ccaaccccta cccacgccca cacacaaaac aaaataaaaa
     1021 acagcgatat acaaaaacca atttgcaaaa acagacctca cgcctatact gccactcaca
     1081 gcaagcaaca caaaaaagcg ggtacaaatt agggtacaaa acacaagccc gaacacagaa
     1141 gcaccccaac caaacccaaa ggcaaaggca aacaaaatgg cggcacacaa caaactgacc
     1201 caaaaacaaa tagaagcagc caaggcagac ggcaaacaaa gcaagctggc cgacggcggc
     1261 ggcttatatc tgctgctgca ccccaacggc agcaaatact ggcggatgcg ctaccggcac
     1321 ggcggatgcg aaaaaaccct agccttgggc gtatatcccg ccgtcagcct caagcaggcc
     1381 cgcgaacttg cccgcgccgc ccgggcgcag accgccgccg gcatcgaccc cgtcgccgaa
     1441 caccaccgca cccgtcccgg cagcgggcgc agccttcccg aaatcgcccg cgcgtggtac
     1501 ggcagccgtc aagggcaacg cgccgacaac acactggaag gcgaccgccg ttcactcgcc
     1561 tacctgaccg actactacaa agacaacacc gacatcaacg acatcaccac cgccggcgta
     1621 tcaaaattta tcgaacacct caaccgcatc aacatccccg cctgcgcccg gcgcaccgtc
     1681 caaatcctcg cccaaatatg ggactatgcc gtccagcgcg gcatcattac cgacgaacgg
     1741 cgcaaccccg caaccaccgc ccgcccactg ctaaacacaa gcaa
//
LOCUS       DAWTHD010000173         1675 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWTHD010000173 DAWTHD010000000
VERSION     DAWTHD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.173"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            40..249
                     /locus_tag="WM704_002109"
     CDS             40..249
                     /locus_tag="WM704_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341437.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
     gene            350..1198
                     /gene="mafB"
                     /locus_tag="WM704_002110"
                     /pseudo
     CDS             350..1198
                     /gene="mafB"
                     /locus_tag="WM704_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            1195..1551
                     /locus_tag="WM704_002111"
     CDS             1195..1551
                     /locus_tag="WM704_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HFA9341438.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
BASE COUNT          603 a          262 c          350 g          460 t
ORIGIN      
        1 cagtattttg atgacaaatt aaaataaaag gaattattca tgaaattaat tgattttgaa
       61 gggaatttag ttaaaattag tctcgataaa gatgagttgt atattattca agcaattgta
      121 ggagaaattt attcaggcgt atgcgtggat tgtagagatt ttgagataat tcatggtgtt
      181 gaaaaaaata aagttcttct tttggataaa gaacttaaaa aaatatatga tacttgggat
      241 aaatgttagc tagatagggt gggcactcgt tgcccacctt ttgccgtata ttaaaaatcc
      301 ttttcaggct gcctgaaact gggtgggcat caatgccaat cctaactgtg cggatgaagc
      361 aggaaaactg atatgggaag acgaccccga taaaaattgg cgggctaacc gtatggatga
      421 tattcgcggc atcgtccaag gtgcggttaa tcctttttta acgggttttc aaggagtagg
      481 gattggggca attacagaca gtgcggtaag cccggtcaca gatacagccg ctcagcagac
      541 tctacaaggt attaatgatt taggaaaatt aagtccggaa gcacaacttg ctgtcgcgag
      601 cctattacag gacagtgcct ttgcggtaaa agacggcatt aattccgcca gacaatgggc
      661 tgatgcccat ccgaatataa cagcaacagc ccaaactgcc cttgccgtag cagaggccgc
      721 aggtacggtt tggcgcggta aaaaagtaga acttaacccg accaaatggg attgggttaa
      781 aaataccggc tatgaaaaac ctgctgcccg acctatgcag actgttgacg gggaaatggc
      841 aggtgggaat aaacctatta aatctttacc aaacagcgcc gctgaaaaaa gaaaacaaag
      901 ttttaagaag tttagtagca actggagttc agcaagtttt gattcagtgc acaaaacact
      961 aactcccaat gcacctggta ttttaagtcc tgataaagtt aaaactcgat acactagttt
     1021 agatggaaaa attacaatta taaaagataa cgaaaacaac tattttagaa tccatgataa
     1081 ttcacgaaaa cagtatcttg attcaaatgg taatgttgtg aaaaccggta atttacaagg
     1141 taagcaagca aaagattatt tacaacaaca aactcatatc aggaacttag acaaatgaat
     1201 gaacacaacc tgttaatttt ctgtttaaaa gacaatgttt caattagtga atatactgaa
     1261 atggttgatt gggcttatga aaacattcaa tctgaaacag ttgtagaaat tacggaaaat
     1321 caaattattg aatatcaaaa tcgtggatta tgggggcttg tttctgaaat taccgataat
     1381 tggttatttg gaccaagtga gggggattgg ctaatagata aggaaagtat tttggctgta
     1441 aaagaaaaat tacaaaattc agatttttct acagagccct tagtgaaaaa tattattcat
     1501 gtacttgaat atgctataaa aaatgaaaaa acagtaattt ttcatttttg aaactaatct
     1561 aatttttagt aatattgatg cagagcaagc agcattagat gccgcaaaca tggggagaag
     1621 ctattcaatt tagaattaaa aaaacaaagt gaaaatggac tagcaccacc aaatt
//
LOCUS       DAWTHD010000174         1664 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWTHD010000174 DAWTHD010000000
VERSION     DAWTHD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.174"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..419
                     /locus_tag="WM704_002112"
     CDS             <1..419
                     /locus_tag="WM704_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341439.1"
                     /translation="LLSESGKTQTVRNIVVKPKPLKAYNLTVADWHTYFVKGDKAETE
                     GVWVHNDCPPKPKPTNHAQQRKEEAKNDSHRSVGDSNRVVREGKQYLDSDTGNHVYVK
                     GDKVVILTPDGRQVTQFKNSKANTSKRVKNGKWTPK"
     gene            397..678
                     /locus_tag="WM704_002113"
     CDS             397..678
                     /locus_tag="WM704_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341440.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            734..1381
                     /locus_tag="WM704_002114"
                     /pseudo
     CDS             734..1381
                     /locus_tag="WM704_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1378..>1664
                     /locus_tag="WM704_002115"
     CDS             1378..>1664
                     /locus_tag="WM704_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341441.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDGDIEVKNLKTEN"
BASE COUNT          589 a          302 c          333 g          440 t
ORIGIN      
        1 ggctgttatc cgaaagcggc aaaacccaaa ccgtccgcaa catcgttgtc aaaccaaaac
       61 cgctcaaagc ctacaatctg accgttgccg attggcatac ctactttgtc aaaggcgata
      121 aggcggaaac ggaaggagtt tgggttcata atgattgtcc gcctaaacca aaaccaacca
      181 atcatgccca acaaagaaaa gaagaagcta aaaacgattc tcatcgaagt gtgggagatt
      241 ccaatcgtgt cgttcgcgaa ggaaagcaat atttagattc cgacacagga aaccatgttt
      301 atgtaaaagg agataaagtg gttattctaa ctcctgatgg aagacaggta actcaattta
      361 agaactcgaa agccaatacg tcaaaaaggg taaaaaatgg gaaatggaca ccaaaataat
      421 caatcttcct tagaaaaaag aattttttat ctggagcatt cggggcagta tctgatgatt
      481 tgcgccctat cggattactc ccaaaataaa catactgtcg ttatggcgaa tttcctctat
      541 ccaaatgaaa aaatggattg gagaaattta gatgatttgt tcaatgagtt ggttttagag
      601 gaattacagt cttcatttat ggattggtat ccaactattg aaaaggcaat tagccatcat
      661 ttagaagact tttcgtaaca agcataaatc aaaaagtcgt ctgaaactgc ttttaatttt
      721 cagacgacct ttccccatat cggcggatta tgaatttaca gctatcggga aattgagtta
      781 tctctctgat agagaaaatt ccatacgcga agccgttgac cggtggatac aggaaaatcc
      841 caatgccgcc gaaaccgtcg aagccgtctt caacgttgcc gccgccaaag tcgcgaagct
      901 ggcaaaggcg gcgaaaccgg ggaaagctgc ggttagcggg gatttttcga ttagctataa
      961 aaacttttct actgtcaaac caaaagtaat tgccaaagga acgattaatg gaaagacttt
     1021 tagagatgtt aatcaaagtg caaaaattgg atcgcctgat tcacctacat taatagccca
     1081 acgagttaat gcaaaaatcc aagccgacgg taaacctcgt ccgaatgcaa ccgttgctaa
     1141 ttcacatgct gaaattggtg ttatccagca ggcttataat gcaggtgaaa cgaaaggggc
     1201 atctatgaca atgacagttt caggaaaaga tgtttgcggt tattgtaaag gagatattgc
     1261 agctgctgct caagcttctg gactgaaatc actgactgtt aatgcgacag ataatgtaac
     1321 aggaaaaaat aaaacttact attggacgcc tggtatgaaa tcaattaagg agagaaaatg
     1381 aaaacatcaa caattgtttt tggtggattt tttattacag ataatggaga acgaatccaa
     1441 atccctattt tagaaaatcc taacattaaa gaaataaata actttttttc tgtatcaaat
     1501 tttgaaaaaa aagccggagt ccttgttttc agaattattc ctgagcctga atttggcaat
     1561 actgaattaa ctatctattt tgaaaaagga tattatttac caataattca aacaatttta
     1621 gaagatggag atattgaagt aaaaaatctt aaaacagaaa atta
//
LOCUS       DAWTHD010000175         1659 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWTHD010000175 DAWTHD010000000
VERSION     DAWTHD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.175"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(56..1477)
                     /locus_tag="WM704_002116"
     CDS             complement(56..1477)
                     /locus_tag="WM704_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFA9341442.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
BASE COUNT          429 a          383 c          506 g          341 t
ORIGIN      
        1 cctttgatta tgccggaatt taaaatgccg tctgaatgtt cagacggcat agcgtttaca
       61 gtagtttgaa aacgaaaaag acgagggtat gtacgatgaa gacgggtgtc aggaaggcga
      121 ccgaccacat catatagccg aagaaagtcg gcatcggtac gccgcgctgt tcggcaatgg
      181 ccttgaccat gaagttcggt gcgttgccga tgtaggtcag tgcgcccatg aataccgaac
      241 ccatagaaac cgccagcagc gaatgaaaca ggggacccgt cattaaggct tgggcatcgc
      301 cgcccgccat attgaaaaac acgagataag tgggcgcgtt atccaagaat gccgacaata
      361 tgccgctcat ccagaaatac atcgtattaa tcggatgacc tgccgtatcg tgaaccagcg
      421 ataccacccc gcccagcgcg cctgcctcgc ctgctttcag aatgctcagg acggggaaga
      481 tggtgatgaa gatgccgagg aagagtttgc ccacttcggc gatgggttca aagttgaatt
      541 cgttgcctgc gcggacttgt ttgggcgtga ttgccataga tacggcggtc aatgtaatca
      601 ggatgacatc gcggacaagg ttttgcaggg cgtaacggct gccgaggatt tcaaatcccg
      661 ggtgttcggg tttccaaagg ccggacatta gaaccgcgcc gaccacgccc gaaagcagga
      721 ggaagttcca tttgccgaag atggcgattt tttcgggttt ttcctgttgt gccggcgtat
      781 cttgtgcaat gctttcctgt ttgaagaaac ggttgtcgat gaaatagaag gcggtcaaca
      841 ggacagcggt gctaatcagg acgggggcga acatatgttt gaccgtccac atgaaatcta
      901 cgcctttgag gaagccgagg aagagtgggg ggtcgcccaa aggggtcagg ccgccgccga
      961 tgtttgcaac caggaaaatg aagaagatga cgatgtgcac gcggcgggta cggtcttggt
     1021 tggctttcag cagcggacga atcatcagca ttgccgcgcc ggtcgttccc atgatagagg
     1081 caagtgccgt accgacggca agcagggcgg tgttgagctt gggtgtgccg ttcaagtcgc
     1141 cccaaaccaa aatgccgcct gaaatggtgt acagggcaag cagcagcagg atgaagggga
     1201 tatattcttc aacgagtgcg tgtgcgacgg tatggatacc ggcggacgcg ccaaaaacca
     1261 aactgaacgg gatgaggaag agcaatgtcc aaaaggcggt gattttgccg taatggtgat
     1321 gccaggtatg cgaaaaaaac aagggaccca atgcgataga cagcaaaatc agggcaaagg
     1381 gcaagcccca cagcaggttt aggtttgcgc cgtccgaatc tgcggcgtaa accgatgccg
     1441 ggaaaagcat tagtgaaaac aggggtaggt ggcgcatcgt gtttcctcga ttcaagcact
     1501 gccttgcgcg gcgcgcggga gtgatacagg cttcgtgccg cccggacata ggcggactgt
     1561 gctgcctgtt tgtttttgaa aaaagttttc cgattgattg taaagtaaat aatcaggata
     1621 aaccagtttc ccaaaccgga aggcggcggg aaggcggat
//
LOCUS       DAWTHD010000176         1636 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWTHD010000176 DAWTHD010000000
VERSION     DAWTHD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.176"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(62..1030)
                     /gene="ispH"
                     /locus_tag="WM704_002117"
                     /gene_synonym="lytB"
     CDS             complement(62..1030)
                     /gene="ispH"
                     /locus_tag="WM704_002117"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFA9341443.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1060..1554)
                     /locus_tag="WM704_002118"
     CDS             complement(1060..1554)
                     /locus_tag="WM704_002118"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HFA9341444.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          355 a          496 c          410 g          375 t
ORIGIN      
        1 ttttgcaaag gtctcggcat ttgtgttgcc caaacatccg atgcctgcgt taacgcctgc
       61 gtcaacgttt gcacaaatcg ggtttggttt cgccctcgcg gcgcagctcc ttgggcagga
      121 cgaacacgat gctttcttcc gcgccccccc cttcgcgcac ggtttcatgc ccccatccgc
      181 gtatggttgc caatacttcc cgcaccaaca cttcgggcgc ggacgcgcct gccgttacgc
      241 cgactttgct tttgccttca aaccacgtgc gttgcaggta ggacgcgttg tccaccatat
      301 acgcatcgat tccgcgcgat gccgccactt cgcgcaggcg gttgctgttg gacgaattgg
      361 gcgaaccgac cacaatcacg atgtcgcact gttccgccag ctctttgacg gcggtttgcc
      421 ggttggtcgt cgcatagcag atgtcttcct tgtgcggatt gcggatattg gggaaacgcg
      481 cgttcagcgc ggcgatgatg tctttggttt catcgaccga gagcgtggtt tggctgacat
      541 aggcgagttt gtcggggttt ctgacttcga gttttgccac atctccgacc gtttcgacca
      601 aaagcatttt gcccggtgca agctgcccca tcgtgccttc gacctcggcg tgccccttat
      661 gcccgatcat gatgatttca cagtcttggg catccagtcg ggcgacttcc ttatgcactt
      721 tcgtcaccag cgggcaagtc gcatcaaata cccggaaacc gcgctccgcc gcttcctgct
      781 gcaccgcctt cgatacgccg tgtgccgaat aaaccagtgt cgcgcccggc ggcacttccg
      841 ccaagtcttc gataaacacc gcgccttttt cgcgcaggtt gtccacgacg aatttgttgt
      901 ggacgacttc gtggcgcaca taaaccggcg cgccgaattc ttccaaagca cgttcgacaa
      961 tactgattgc ccgatccaca ccggcgcaga agccgcgcgg attggcaagg atgatggttt
     1021 ttccgttcat aagttttgca ttccgtgttc agacggcatt cacgtttttt tgctatcttt
     1081 gcgatggacg atattgtcaa gcaccgccaa caccgcaccg acgcagataa agctgtcggc
     1141 aatattaaag gcgggataaa accaattttg ccaataaaac aataagaaat cgacgacatg
     1201 accgtgtatc aggcgatcga tgacattgcc cgacgcaccg ccgataatca ttgccgcccc
     1261 gattttgccg agggctgcaa actcgtcgcg caagatggcg cgtaccaaat acgcgctcac
     1321 cgccaccgcc agcaccaaaa aaaagtattt ctgccagccg ccctgatcgg caaggaagct
     1381 gaatgccgcg cccggattgt acaccagcgt cagatcgaaa aacgaaggga tgacgttgac
     1441 gcgttcccga tactgaaacg acgacagcac cgcccacttc gaccactggt ccagcacgat
     1501 ggcggcaagt gccaataccc aatagcgcgt tttacttgaa acagatgaag acatattttt
     1561 caacagccgg ataaaagagt gccattctac ccgaaaaccc cctttcctgt acccgaaacg
     1621 gcaaatgccg taatct
//
LOCUS       DAWTHD010000177         1564 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWTHD010000177 DAWTHD010000000
VERSION     DAWTHD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.177"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            40..234
                     /gene="thiS"
                     /locus_tag="WM704_002119"
     CDS             40..234
                     /gene="thiS"
                     /locus_tag="WM704_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFA9341445.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
     gene            313..1101
                     /locus_tag="WM704_002120"
     CDS             313..1101
                     /locus_tag="WM704_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFA9341446.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            1080..1328
                     /locus_tag="WM704_002121"
     CDS             1080..1328
                     /locus_tag="WM704_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFA9341447.1"
                     /translation="MAFGGILKKAAKMPSEGFRRHRGPKRANRHSPASRLCREEQGNR
                     PENLAARPDTATNETPGLPGAQAAAQALSTAIEGSFVV"
BASE COUNT          359 a          513 c          414 g          278 t
ORIGIN      
        1 tttgttaatc cgctataaat acagaaacat cgagaaacca tgaacatcat cttaaacggc
       61 ggacccgccg aacttcacgg cacaagcgtt gccgacctca tcgcccaaac cgcgccgcaa
      121 aaaccctttg ccgtggcggt caacaccgtt ttcatcccca aaggcgcgta tacggaaacc
      181 gttttacacg aaaacgacaa aatcgacatc gtccgccccg tcgtcggcgg ctgaatgccg
      241 tctgaaaccc caaaggcacg ccgccggctt tcagacggca tcgcgtccga caaccatacc
      301 aaagaatcca tcatgctcac cctgtacggc gaaactttcc cttcgcggct gctgctcggc
      361 acagccgcct acccgacccc tgaaatcctc aaacaatccg tccgaaccgc ccggcccgcg
      421 atgattaccg tctcgctgcg ccgcacggga tgcggcggcg aggcgcacgg tcaggggttt
      481 tggtcgctgc ttcaagaaac cggcgttccc gtcctgccga acacggcagg ctgccaaagc
      541 gtgcaggaag cggtaacgac ggcgcaaatg gcgcgcgaag tgtttgaaac cgattggata
      601 aaattggaac tcatcggcga cgacgacacc ttgcagccgg acgtgttcca actcgtcgaa
      661 gcggcggaaa tcctgattaa agacggcttc aaagtgttgc cttattgcac cgaagacctg
      721 attgcctgcc gccgcctgct cgatgcgggc tgtcaggcgt tgatgccgtg ggcggctccc
      781 atcggcacgg gtttgggggc ggttcacgcc tatgcgctca aaatcctgcg cgaacgcctg
      841 cccgacacgc cgctgattat cgacgcgggc ttgggtttgc cttcccaagc ggcacaagtg
      901 atggaatggg gttttgacgg cgtattgtta aacaccgccg tttcccgcag cggcgacccc
      961 gtcaacatgg cgcgcgcctt cgcactcgcc gtcgaatccg gacggctggc atttgaagcc
     1021 gggccggtcg aagcgcgaac caaagcccaa gccagcacgc cgacagtcgg gcaaccgttt
     1081 tggcattcgg cggaatattg aaaaaggcgg caaaaatgcc gtctgaaggc ttcagacggc
     1141 atcgcggccc caaacgggca aaccgccatt ccccggcatc acggctttgt cgtgaggaac
     1201 agggaaaccg gccggaaaac cttgccgccc gccccgacac cgcaaccaac gaaacacccg
     1261 gactccccgg cgcgcaggct gctgcgcaag ccctaagtac ggcaatagaa gggagttttg
     1321 tcgtttgaat acatttaaaa atacctcggg gcatccgtaa gccttcatcc gcaacggttt
     1381 caccgctttt gcatccccga atccgcgctc agacaccccc gaatggcaac cctgtccgca
     1441 ccaaatcgga cggttgttca aacacggcag gcttctttcc gacaggcacg aagcccccgt
     1501 ccatgcctgc cgaccccgat ttgtccggcg caatgaaagt ttgccgaccc aaatcacaaa
     1561 catc
//
LOCUS       DAWTHD010000178         1484 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWTHD010000178 DAWTHD010000000
VERSION     DAWTHD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.178"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            53..322
                     /locus_tag="WM704_002122"
     CDS             53..322
                     /locus_tag="WM704_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341448.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGILPKPVALSIVQYEND
                     PGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="WM704_002123"
     CDS             304..456
                     /locus_tag="WM704_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341449.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="WM704_002124"
     CDS             443..988
                     /locus_tag="WM704_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFA9341450.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPFAW"
BASE COUNT          478 a          256 c          320 g          430 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgtagtaac aattattcag gattagaaaa ttatgagtgt
       61 aaaagaatta ttcaaagtta tcttagataa aaataaagat tttcctatta gaacgattca
      121 tcgcactcca atggggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaaa
      181 tgacccggga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggctgaattt gaatttggaa tcagcaaaga
      301 ggaatggatg caaagtcctt agacggcaaa atcaagatgc cgctttattt ttcagatggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacgtct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgatga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagcagaat tggtggaaaa
      901 gaaaggttgg aatgcttttg agcagttctt gctggataat gaagttgaca acctttcgga
      961 tatgaagcgg aaaccgtttg cttggtaagg gctttcttaa atttgattaa aggtcgtctg
     1021 aaattaagct tgtttttcag atggtctttc cttcatttga agcaggatat tgagaactga
     1081 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1141 ggctgaaaag caaacaccgt ccgtcgtgtt gccgtttgcg gatgagtacg ggtcaacccc
     1201 aatgccgccg aaaccgtcga agccgccttc aacattgcca ccgccaaagc cgcgaagttg
     1261 gcaaaaacgg taaaaccggg gagataaaag ccgatggcag gaaagtaaat gtgaggatag
     1321 acagtacgga ggcagacctg ctttatccgg cagggcaata agaaaacaaa acttagatat
     1381 ggaaaacgat tgtgaagatt aaaccattac aattttctaa caataatcac agattttatg
     1441 tggacaaaat ttgtgttacc gaacaagatg tgaacatttt ggca
//
LOCUS       DAWTHD010000179         1479 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWTHD010000179 DAWTHD010000000
VERSION     DAWTHD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.179"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..1169
                     /gene="mafB"
                     /locus_tag="WM704_002125"
     CDS             <1..1169
                     /gene="mafB"
                     /locus_tag="WM704_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9341451.1"
                     /translation="VDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARDIYSYY
                     VKGTSTKTKINTVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWENNPDKNWWGNRM
                     DDVRGIVQGAVNPFLTGFQGLGVGAITDSAVNPVTDTAAQQTLQGINDLGNLSPEAQL
                     AAASLLQDSAFAVKDGINSAKQWADAHPNITATAQTALAVAEAAATVWRGKKVKLNPT
                     KWDWVKNTGYKKPAARPMQTVDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDT
                     SISPTGTIQDRIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSD
                     KILGYQTEHRRVRYDINNNIYVLANPKTFKIKTMFKPDLGKEYYDGEFKKDMGN"
     gene            1135..1341
                     /locus_tag="WM704_002126"
     CDS             1135..1341
                     /locus_tag="WM704_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341452.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
BASE COUNT          497 a          307 c          332 g          343 t
ORIGIN      
        1 gcgtagacgg cggttttacc gtttaccaac ttcatcggac agggtcggaa atccatccgg
       61 aggatggata tgacgggccg caaggcagcg attatccgcc ccccggagga gcaagggata
      121 tatacagcta ctatgtcaaa ggaacttcta caaaaacaaa gataaacact gttccgcaag
      181 cccctttttc agaccgctgg ctaaaagaaa atgccggtgc cgcttccggt tttttcagcc
      241 gtgctgatga agcaggaaaa ctgatatggg aaaacaaccc cgataaaaat tggtggggta
      301 accgtatgga tgatgttcgc ggcatcgtcc aaggtgcggt taatcctttt ttaacgggtt
      361 ttcagggatt gggagttggg gcaattacag acagtgcggt aaacccggtc acagatacag
      421 ccgctcagca gactctacaa ggtattaatg atttaggaaa tttaagtccg gaagcacaac
      481 ttgccgccgc gagcctatta caggacagtg cttttgcggt aaaagacggc atcaattccg
      541 ccaagcaatg ggctgatgcc catccgaaca ttacagctac tgcccaaact gcccttgccg
      601 tagcagaggc cgcagctacg gtttggagag gtaaaaaagt aaaacttaac ccgaccaaat
      661 gggattgggt taaaaatacc ggctataaaa aacctgctgc ccgacctatg cagactgtag
      721 acggggaaat ggccgggaaa aataagccac cgaaaccaag tacgcagcaa cactctacac
      781 actctgataa caatatcggc ttacctgccc catatgttaa acctgataca tctatttctc
      841 cgacaggaac aattcaagac cgcatcagat ggacaaaatc caagtttcct actgagaaat
      901 ctttaaatgg acatttcaaa gctcatggaa aagaatttgg cgatataacc attgaagact
      961 accaaaaaat ggcgtctgat ttgttatcaa aacagacatc ggacaagata ttaggttatc
     1021 agacggaaca tagacgagtg cgctatgata tcaataacaa tatctatgtt ttggccaatc
     1081 caaaaacatt caaaatcaaa acaatgttta aaccagactt aggaaaggag tattatgatg
     1141 gagaattcaa aaaagacatg ggaaattgac ggagaaatat ggctacattg tcctgtttgc
     1201 ggaactgaag ttatggacta tgatatctgt gacgtttgtc agtggcaaaa tacaggagaa
     1261 actaatatag atggtggtcc taatgaaatg acacttgcgg aggcgaaaga agcttacgca
     1321 aaaggcttac caatcagata aataagcacc tagagaaacc aatgatgacg gaaccccatg
     1381 gttacccatc aaataattta cctgttaaaa tcataaatga tattatttat gcaacccagt
     1441 tagtcgaaga tttggtttta ggaaaaataa aaattgttg
//
LOCUS       DAWTHD010000180         1477 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWTHD010000180 DAWTHD010000000
VERSION     DAWTHD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.180"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(1..256)
                     /locus_tag="WM704_002127"
                     /pseudo
     CDS             complement(1..256)
                     /locus_tag="WM704_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            446..790
                     /locus_tag="WM704_002128"
     CDS             446..790
                     /locus_tag="WM704_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFA9341453.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            744..1277
                     /locus_tag="WM704_002129"
     CDS             744..1277
                     /locus_tag="WM704_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFA9341454.1"
                     /translation="MWEILFRYQDFVAINKPQGISVHRSGGEVSLTATLAAQLGVEKV
                     WLLHRLDKQAGGILLFALNPQSAAVLAAQFAERKMKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNMENIAVTRFFSIRISEKMRLFILEPHTGKTHQLRVVMKSLGSSIF
                     GDSLYGGTESETMFLYA"
BASE COUNT          451 a          305 c          303 g          418 t
ORIGIN      
        1 ttattttgtg ccttactttc agtaccaatt gccaccgcac ttaaatttgt tgcctgagaa
       61 gatgtcccca ctgcaattcc tttcgtacca ctggcagcac tgcttttccc taaggaaata
      121 ccaaaattcg atgcattagc agaagtaccc aatgccactc aattttagat gccgatgctt
      181 tatcgttcca tgataaggag ccattattac cttcttgcaa agtagaatcc gccccataag
      241 aagacaaagc actcattaac aaaataccag ccgctatcgc ggacgaccta tatacataac
      301 attacttcat atcatttcat tcagtatcaa tagtatagga taactccccc cccccgtcaa
      361 tcgaaataat tcccgtattt taatgtcgta ttgttcctgt atatattagt gatgtgataa
      421 gtatagttta ttttttgagg tttttatgaa tttacctttt cgggcaatgg tgtctgattt
      481 aggtggcaca cttttaacac ctgaacattt agtgggtgat cttaccattg atactctgag
      541 agtattagag caaaaaggcg tagatattat tttggcaaca ggacgcaatc ataccgatat
      601 gtcatccatt cttggaaaaa tcggtgcgga acgtgcggtt atgattacat caaacggtgc
      661 tcgtgtacgg gatttacagg gtaatttgct ttatagcaat agtttgcctg aagaattagt
      721 tctcgagctt tacaaaacat catatgtggg aaatcttatt cagataccaa gattttgtcg
      781 ccatcaataa acctcaaggc atatccgtcc accgttccgg cggcgaagtc agtctgacgg
      841 caacacttgc ggcacagttg ggcgtagaaa aggtatggtt gctgcacagg ttggacaagc
      901 aggccggagg catattgctg tttgccctta atccccaaag tgccgctgtt cttgccgcac
      961 agtttgccga aaggaaaatg aagaaaacct atttggcttt gtccgaccgc aaaccgtcca
     1021 aaaaacaagg atggataaaa ggcggcatgg aaaaatccag gcgcgggatg tggaagctga
     1081 cgcgcaatat ggaaaatatt gccgttaccc ggtttttcag tatccgcatc agtgaaaaaa
     1141 tgcggctgtt catccttgag ccacatacgg gtaagacaca tcagttgagg gtggtaatga
     1201 agagtctggg cagctctata tttggggaca gcctgtatgg aggaacagaa tccgaaacta
     1261 tgttcctgta tgcgtgaaaa atacaattta cctatcaaca acaagaaatc gaaattaccg
     1321 cacctttgaa aaatgcatgg caggccgaaa atatctctca tgcactggaa gaattttata
     1381 tggcaaaagc aggttaatag atataaatca attaaaacaa tatgataaat atttttataa
     1441 aattttttca aaaaacggtt gaccgcaaat ctcttta
//
LOCUS       DAWTHD010000181         1447 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWTHD010000181 DAWTHD010000000
VERSION     DAWTHD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.181"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(605..886)
                     /locus_tag="WM704_002130"
     CDS             complement(605..886)
                     /locus_tag="WM704_002130"
                     /inference="COORDINATES: protein motif:HMM:NF021923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFA9341455.1"
                     /translation="MSNDENLLFSQPHLTKVNIMQVLTLDEIQQVSGAACNWRDFSKN
                     TIGSALGGAAGGAIVGSFAGGIGAIPGAKFGAIGGAITGSVQYGSTCWW"
     gene            complement(1107..1343)
                     /locus_tag="WM704_002131"
     CDS             complement(1107..1343)
                     /locus_tag="WM704_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFA9341456.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGVSYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
BASE COUNT          489 a          263 c          225 g          470 t
ORIGIN      
        1 caataatcca gaaatggttg cagctaaaat gcaaaagaca gtggcgtaaa tggtttcagt
       61 attcacaatt tttctctttc aaaattgttc aatctaaata gaaaaaggta acttcaatac
      121 atattgctgt aactgaaatt accttttcct taatgatgta attatcttat ggcttcatca
      181 tgccacgacc gatattgtat ccgccagcaa cagagaaaat accagctccc attgcgtcaa
      241 cccagcctac tggtcctgtc cacttgttcc gttgagctac aaagacttct ggtctgaaaa
      301 atgagtgatt gttagacatg tgtacttatt tatgtttctt tgattgggat aaatttgtaa
      361 aaacaaaaaa aaggcaagtt gttattatag aagaacttaa aatgcttaac atactcattg
      421 aggtttgtac cacataatga gatacgagat aatttgttgg gagtaaaatc aaaaaaaaga
      481 ttaaatattt aaaataatca ctatattttt tcattatttg ttcctttttg tatttaagtt
      541 cttatgaaaa actagggtat tttgaataga aaatatcaaa ataccctagt tttattaagg
      601 aatattacca ccaacaagtg cttccatatt gtacagaacc agtgattgca ccaccaatag
      661 ctccgaattt cgcacctgga atagcaccaa taccacctgc aaatgaacca acaattgccc
      721 caccagctgc tccacctaat gcactaccaa tggtattttt tgagaaatca cgccagttac
      781 aagcagcacc agaaacttgt tgaatttcat ccaaagtcaa aacttgcata atattcacct
      841 ttgttaagtg tggttgagaa aaaagtaaat tttcatcgtt actcattatt tgcaatcatg
      901 catatactgt aaccaagcac tatattcagt tggatcatag tttttactac tgggttgtct
      961 gatatgacta ttggcactat tggcacaact gggattagca actttattgt gagtacctaa
     1021 tggattgata tttcctgtaa agtggctatt gcatgcgctt aatgagcata gatttattaa
     1081 tgtcagaaag gataaaacaa aaaatcttac cattttttac taaaaccaaa agtaggtttc
     1141 gaaaactgta caccaagacc ttgtacatat ccaaattgct tgctaacaga aaactctact
     1201 ccactattgt taggggaata ggaaacacca attttaccaa cagcaaccat atctttttta
     1261 cctaggttgc cgccaccctg tgcagagata tggaatttgc cacctgacac ttcaactaat
     1321 tcagaggtgt gtaactcttt catgattttc tcctgtaaag aagtatggtt ggaaatgtaa
     1381 atcagaatca atctttctta agtttaattt aatacttttt tttgcaagta ttttatttta
     1441 aattaaa
//
LOCUS       DAWTHD010000182         1387 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWTHD010000182 DAWTHD010000000
VERSION     DAWTHD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.182"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(46..1029)
                     /gene="porB"
                     /locus_tag="WM704_002132"
     CDS             complement(46..1029)
                     /gene="porB"
                     /locus_tag="WM704_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFA9341457.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHHRAQA
                     DRVKTATEIADLGSKIGFKGQEDLGNGLKAIWQLEQKAYVSGTDTGWGNRQSFIGLKG
                     GFGKVRVGRLNNILKDTGGFNPWEGKSYYLGLSNIAQPEERRVSVRYDSPEFAGFSGS
                     VQYVPNDNSGKNHSESYHAGFNYKNSGFFVQYAGSYKRHNFTTEKHQVHRLVGGYDHD
                     ALYASVAVQQQDAKLAWSNDNSHNSQTEVATTVAYRFGNVTPRVSYAHGFKGSVYDAD
                     NDNTYDQVVVGAEYDFSKRTSALVSAGWLQRGKGTEKFVATVGGVGLRHKF"
BASE COUNT          312 a          314 c          373 g          388 t
ORIGIN      
        1 agcctgtctg acgacaggct ttttgttgat accgatcttt gcagattaga atttgtggcg
       61 cagaccgaca ccgccgacag tcgctacgaa tttttctgtg cctttgcctc tttgcaacca
      121 accggcagaa accaaggcag aagtgcgttt ggagaagtcg tattccgcac cgacaaccac
      181 ttggtcgtaa gtattgtcgt tatctgcatc ataaaccgaa cctttgaagc cgtgggcgta
      241 agaaacgcgg ggcgttacgt tgccgaagcg gtatgccacg gtagtggcaa cttcggtttg
      301 agagttgtgc gaattatcgt tggaccaagc caatttcgcg tcttgttgct gtacggctac
      361 ggaagcgtac agggcatcat ggtcgtaacc gccgaccaaa cggtgaacct ggtgtttctc
      421 agtcgtgaaa ttatgtcttt tataggagcc ggcatattgc acgaagaagc cgctgttttt
      481 gtagttgaag cctgcatggt aagattcgct gtgatttttg cccgaattgt cgttaggcac
      541 gtattgtacg ctgccgctga agccggcaaa ttcgggagaa tcgtagcgta cggaaacgcg
      601 gcgttcttcg ggttgggcaa tgttgcttaa acccaaatag tagcttttac cctcccaagg
      661 attgaagccg ccggtgtctt tcaggatgtt gttcaaacgg ccgacgcgca ctttaccgaa
      721 gccgcctttc aaaccgatga aggattggcg gttgccccag cctgtgtcag taccgctgac
      781 gtaggctttt tgttccaact gccaaatggc tttcaggccg ttgccgaggt cttcttgacc
      841 tttaaagccg attttcgaac ccaaatcagc gatttcggta gcggttttaa cgcgatccgc
      901 ctgagctcta tggtgagcta cggagcggga agtttctacg ccggctttga tggtgccgta
      961 cagggtaacg tcagccattg ctgcaacagg aagggctgcc aaagtcaggg caatcaggga
     1021 ttttttcatt gctgtattcc ttttttggtt aagaaattta agcaggccgg gctttccaag
     1081 ccgcttagct ttgcatttac cgccgagggg ctcggcggtt acatgtctga aactatagat
     1141 gttcctgtct gaaaaaacaa acttctgcct gtttttgtgt ggaatccgat gtgcattttg
     1201 aagggcggat agagatatta cgggtatttt ttgttttgta acatatggtt attttaaatt
     1261 tttaagattt gcatttttac aacacttact cgggagggta ttggagggca ttgcaaaccg
     1321 ggggttatag cggattaaca aaaaccggta cggcgttgcc ccgccccggc tcaaagggaa
     1381 cggttcc
//
LOCUS       DAWTHD010000183         1360 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWTHD010000183 DAWTHD010000000
VERSION     DAWTHD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.183"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(393..803)
                     /locus_tag="WM704_002133"
     CDS             complement(393..803)
                     /locus_tag="WM704_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341458.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(807..>1360)
                     /locus_tag="WM704_002134"
     CDS             complement(807..>1360)
                     /locus_tag="WM704_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9341459.1"
                     /translation="WRGKKVELNPTKWDWVKNTGYKKPAARHMQTLDGEMAGGNKPAK
                     QATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMVRHKPDASS
                     LDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDVVGYTDGKPTHII
                     NIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          391 a          255 c          226 g          488 t
ORIGIN      
        1 tgttatcagc ctctacggat ggggcggagt tacccgtatg attgccgtat ggttggatac
       61 ttgtatccga caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttggaaaat
      121 gtcggattcg agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa
      181 tccatgtttc gccattttcc tttatatata cctaatgcag cacgggttac gatttcgttt
      241 aaatcataaa tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa
      301 acccaacaaa aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg
      361 atttaggttt taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgc
      421 actgaacaaa caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa
      481 atgtttacat ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca
      541 tgaggatatt tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt
      601 aattttgcac acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca
      661 attccttcat cccaatcaaa agtaaaattc gttccttcta tgataaattt agcacaagat
      721 attaaatcta acttaaccca agtggattta tacagactca atgctgatga agttataata
      781 ttatctaaat tataaatttt cataaatcaa cgttccttac ctggtgttcc atgcacaggt
      841 ctaatcccat ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg
      901 tctgtatagc caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa
      961 ttatgtttat ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat
     1021 ttaaataaac ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtacc
     1081 atactgagag ggtcagtaat atatttgcca tctgtaatag ctgatccacc attataggta
     1141 gtatgcgtat aatttttttg tggaacgaga ctattttgca aagattgttg tgtagaaacg
     1201 ccagctttgc cattaggagt agcttgcttc gctggcttat tcccacctgc catctcccca
     1261 tctaaagtct gcatatggcg ggcagcaggt tttttatagc cggtattttt aacccaatcc
     1321 catttagtcg ggttaagttc tactttttta cctctccaaa
//
LOCUS       DAWTHD010000184         1271 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWTHD010000184 DAWTHD010000000
VERSION     DAWTHD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.184"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(12..209)
                     /locus_tag="WM704_002135"
     CDS             complement(12..209)
                     /locus_tag="WM704_002135"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFA9341460.1"
                     /translation="MDNAAMESFFGTLKSECFHTCKYDSVTESEAALHEYIRYYNNDR
                     IKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="WM704_002136"
     CDS             complement(254..832)
                     /locus_tag="WM704_002136"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFA9341461.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..1265)
                     /locus_tag="WM704_002137"
     CDS             complement(837..1265)
                     /locus_tag="WM704_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9341462.1"
                     /translation="MSDSLVRKWVARYRLHGESGIKRRKHTTKYSVEYKLEAIRPVTG
                     QGMSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADY
                     LKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
BASE COUNT          273 a          325 c          289 g          384 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactcg gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttcggcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgacg agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcagtcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtc
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa a
//
LOCUS       DAWTHD010000185         1249 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWTHD010000185 DAWTHD010000000
VERSION     DAWTHD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.185"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            29..481
                     /locus_tag="WM704_002138"
     CDS             29..481
                     /locus_tag="WM704_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341463.1"
                     /translation="MQTKALGTVDEIGNTVQQVGKQTSGQKTSGGNPAIDSDPYSPSS
                     VSARIEAGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDGRAVNVRNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            485..886
                     /locus_tag="WM704_002139"
     CDS             485..886
                     /locus_tag="WM704_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341464.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
BASE COUNT          411 a          199 c          267 g          372 t
ORIGIN      
        1 cggttataaa aaacctactg cccgccatat gcagactaag gcgttaggta cggtagatga
       61 aattggcaat acagtacagc aggttgggaa acagactagc ggacaaaaaa ccagcggtgg
      121 taatcctgcg attgatagcg acccctatag cccgagtagt gtgtcagctc gtatagaagc
      181 cggtaaggtg cgcagtgatt tacaaatcaa agacattttg agcaatacta ctcaaaggag
      241 taaaacaaaa ggtcccgctg ttcagtatga taaagtgggg gattacaatg acgcactaaa
      301 tgattttaat agtctgaatg ttcgaaatgt acaaacacgt cctaatggaa cgataacggg
      361 caatttacct gatgggcgtg cggttaatgt tcgtaatgat agcagtggtg gagaaccaac
      421 acttgaaata acaattagta ataaccgaaa aataaaaatc agatatggaa atacacgata
      481 aattatgaaa ttaaaaagct tagatttccc aactggctat ttctattttg ataatgcagc
      541 aataaacttt gataaagtag aagttatagc agttggttat agaaatacgg gtaaaaccat
      601 aaaaatttct attgaggatg ttattcattt tagggttgtt gatgaatcgt attttataga
      661 tacttttatg gatttaattt cggaagatgc agatagagct ttgcttcatg aaaatggtgg
      721 tcaatctttt ttcgaacttc ttgatgagcg ttatgcggca tggatatcga aagaaagtta
      781 ttttcctttg aatagagaat tctttaaata ctatattttt atgtttgagc aaacattcat
      841 agaaataatt ggttctagtg caacgtattc aattattgag agctagcgta agatgagtaa
      901 taagttgcct atctttcttt caggcagcct gaaaataaaa ttactcgtgt aatgaaccac
      961 gcactttaaa cagcacaggt attttgatga gaatggcatg gcaattaagt atattggttt
     1021 ctacattccg tccgcaatat tcgcgccttc cgcattgaac aatggtcaga ctttacggat
     1081 atagccgtag gttggattct cgaatccgac attttctaac agcggcattt cggaaacgat
     1141 aaacgcgtca aatatttttg tcggatacaa atatccgacc tacatctctg cgatggggta
     1201 cctgtatcag tgaagggaaa ttttgttgat ggtaaatttc gcattggta
//
LOCUS       DAWTHD010000186         1231 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWTHD010000186 DAWTHD010000000
VERSION     DAWTHD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.186"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(242..1092)
                     /locus_tag="WM704_002140"
                     /pseudo
     CDS             complement(242..1092)
                     /locus_tag="WM704_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
BASE COUNT          254 a          290 c          361 g          326 t
ORIGIN      
        1 caccaacctc accggctcaa accggcacag caacttatat cccgtcattc tcgcgcaggc
       61 gggaatccag ccccgccggt acagaaactt atcgaaaaaa agtttcttca gattttacgt
      121 tctagattcc cactttcgcg ggaatgacgg gatgcaggtt tccgcgcgga cggattcggc
      181 attcccgctt tcgcagaaat gtcggctttt ggtttgttgt ctttataaga ttattcggta
      241 attattctgc ggttttgcgt ttgtgtgctg ccggaatctc aagggattct ctgtatttgg
      301 caacggtgcg gcgggaaacc tctataccgc ggaaagccag caggttggcg agtgcctcgt
      361 cagaatagga cttgggcgtg gacgaggcaa aaccggcggc gggaaagggg ttgagcgaag
      421 caatgaggcc gagtgcggct tcgagtgtgc cgctgtcggt ttggggcagg cgttttttat
      481 tcgcgccggg gtttggctgc ggttgccgtc aatgctgtcg agggcgtttc ggacgatatg
      541 cagggcggcg ggtttggcag cacattcgcc cgatctttct atctgcaata tcaacgattc
      601 ggtcacatcg gcggcggcca tgcctgccgg atcgaatttt ttcaatgcgg tcagggcttg
      661 tttcagcatt gcttcatcca acatccactc caagggcgta tggtccagga tgtcttcgat
      721 gctgtcggtc aggtagccct gctcgtcaag gaaatcgata aggatgtgga cgcaggcggc
      781 ttcttggtcg gaaagggggt gttcgcacgc ttgcgcgtgc aggtattgct tgaaatcttc
      841 ttcgccggcg atgttggaca gcatatcttc gccttcgtct ccgccgattt gacgcgcggg
      901 cgcggtgtaa tggctgaatt cggcatcgga aaattcatcc gtttctttgc gttcgagcag
      961 ggggttgtcc gacggtcagt tttcgacctc gcgttcaagt tcgatacccg gcatctgcaa
     1021 tacgcgcaaa gattgttgca gccgctggtc gagctgctgg gtctgtttga gctttattcc
     1081 gattaaggta atgataatgc ggtgaaaatt gttattttca gcctgtcggc gcaaaaaaat
     1141 gccgcaaagc ggcattgcat tataaatggt tttaatgagc gggttcggat tccgtccgat
     1201 aacaaaaaac aaacgaaaat ggaaacttga t
//
LOCUS       DAWTHD010000187         1222 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWTHD010000187 DAWTHD010000000
VERSION     DAWTHD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.187"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            164..310
                     /locus_tag="WM704_002141"
     CDS             164..310
                     /locus_tag="WM704_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341465.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(687..1136)
                     /locus_tag="WM704_002142"
     CDS             complement(687..1136)
                     /locus_tag="WM704_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFA9341466.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          379 a          301 c          186 g          356 t
ORIGIN      
        1 acgatcggat atcttcaaat gcacatttca cagaaaggtc gtctgaaacc cattttcaga
       61 cgaccttttt ccacttcgca aaacaagaat tcccacccct ctccccaaac atcgcccaaa
      121 ccagtaaatt ccccatattt caacaactcc ttggaagcaa accatgtccc gtatctacct
      181 acccctccta tttccgcccc acatcgtcga acgcggcctt ttgtattttc agcaggacaa
      241 ggttcgcgat gtccaaaaaa tttccttagg gcgttatcgg gcgaaggtat tcgattcgga
      301 aaactattag gcatggttga agctggatag tgatttgtac attaaggacg gggctgcgat
      361 tgcccttatg tctaaaggtg caaacatgcc ttaaattact aagcctataa cgaattggaa
      421 acaaaatgcc gtctgaaaac atcttcagac ggcattataa aatctgttca ccttttcaga
      481 tgagtaatgt acacccctat acaatttttg ctaccatgca ccataaatcc acggctaaag
      541 ataattgggt ttattctttt atccagaaac aatccagata cggatcacgg gtcataacta
      601 taggcttaat attacacgat tctcattcca tcaaggcgga aaaccgcaca aatactgaaa
      661 cactatcgat cgatttgtaa acaagcctac ttaagtaact tgcagtcctt atcatttcct
      721 ttaaaataat ccagcccgtc actacacgaa ctggcggact tcttgcaaat aaaggttact
      781 agattttcat tcatcttaat aataaaagga tttttatctt tatctatggc taccgccttc
      841 aacatgaatt tactgtctaa agccccgcgc gcgattccat tcaaacggat acaaaagcct
      901 tctgcctctt taatcggcaa acttggccat ttggtagatg tttgtttaaa tctcccattc
      961 tgcagataaa acttttccat aaaatgtgca ttttctaaca aggctgcccg cactgcattt
     1021 atctttgctt tctcaacata attgcgatag ctcggataaa caattaaagc aagtacagac
     1081 aatatcaaga ccactgatat taattcaacc agcgtaaacc cccgattatc agtcattact
     1141 ttacttccaa taagaacaga ttattcaaca tatttctttg aacagactta ctatcccatt
     1201 cagcagtatg catatttccc ac
//
LOCUS       DAWTHD010000188         1219 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWTHD010000188 DAWTHD010000000
VERSION     DAWTHD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.188"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            21..836
                     /locus_tag="WM704_002143"
     CDS             21..836
                     /locus_tag="WM704_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9341467.1"
                     /translation="MNPARKKPSLLFSSLLFSSLLFSSAAQAASEDGGRGPYVQADLA
                     YAYEHITHDYPDAAGANQGKKISTVSDYFKNIRTHSIHPRVSVGYDFGGWRIAADYAR
                     YRKWNNNKYSVNTKKVGENKDNKKRITVTQYLESENQENGTFHAASSLGLSAVYDFDT
                     GSRFKPYAGVRVAYGHVKHQVRSVEKETTFVLSKTLGVEKPGIIKDAPTRKLPHHESR
                     SISSLGFGAVAGVGIDITPNLTLDAGYRYHNWGRLENTRFKTHEASLGMRYRF"
     gene            850..999
                     /locus_tag="WM704_002144"
     CDS             850..999
                     /locus_tag="WM704_002144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341468.1"
                     /translation="MPSEPSDGIFDAIAVYRRGAGAVKYPNRHSRQYRNLETRHSRAG
                     GNPDP"
BASE COUNT          318 a          372 c          289 g          240 t
ORIGIN      
        1 aaccgaataa ggagccgaaa atgaatccag cccgcaaaaa accttctctt ctcttctctt
       61 ctcttctctt ctcttctctt ctcttctctt ccgcagcgca ggcggcaagt gaagacggcg
      121 gccgcggccc gtatgtgcag gcggatttag cctacgccta cgagcacatt acccacgatt
      181 atcccgatgc agccggtgca aaccaaggca aaaaaataag cacggtaagc gattatttca
      241 aaaacatccg tacgcattcc atccacccca gggtgtcggt cggctacgac ttcggcggct
      301 ggaggatagc ggcagattat gcccgttaca gaaaatggaa caacaataaa tattccgtca
      361 acacaaaaaa ggtgggagaa aacaaagaca ataaaaaaag gataaccgtg acgcaatatc
      421 tggagtcgga aaatcaggaa aacggtacgt tccacgccgc ctcctcgctc ggtttgtccg
      481 ccgtttacga tttcgatacc ggttcccgct tcaaacccta tgcaggcgtg cgcgtcgcct
      541 acggacacgt taaacatcag gttcgttcag tggaaaaaga aaccacgttt gtactcagta
      601 aaacacttgg cgttgaaaag ccaggcatta tcaaagacgc tccgacccgc aaacttcccc
      661 atcacgaaag ccgcagcatc agcagcttgg gcttcggcgc agtggcaggc gtaggcatcg
      721 acatcacgcc caacctgacc ttggacgccg gctaccgcta ccacaactgg ggacgcttgg
      781 aaaacacccg cttcaaaacc cacgaagcct cattgggcat gcgctaccgc ttctgattcc
      841 ccgacaccga tgccgtctga accttcagac ggcatttttg atgcgatcgc cgtttacagg
      901 cgcggggcgg gcgcagtaaa atacccgaac cgtcattccc gacaataccg caatctcgaa
      961 acccgtcatt cccgcgcagg cgggaatccg gacccctgac gcggcgggaa tctatcggaa
     1021 atgactgaaa ccccgcgtcc tagattccca ctttcgcggg aatgacggtt cggttgctac
     1081 ggcccgctga ttccccgaca ccgatgccgt ctgaaccttc agacggcatt tttgatgcac
     1141 ctgccgttta cagacgcggg gcgggcgcag taaaataccc gaaccgtcat tcccgacaac
     1201 accgtaatct cgaaacccg
//
LOCUS       DAWTHD010000189         1210 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWTHD010000189 DAWTHD010000000
VERSION     DAWTHD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.189"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            107..355
                     /locus_tag="WM704_002145"
     CDS             107..355
                     /locus_tag="WM704_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341469.1"
                     /translation="MLKTKRTNCLKKDLIGMRVFPTLSPFIYPHYKNMKKTLLALSLA
                     AVSAVTVADIPESNGKGEYKVFTAYVKGKATAGNLKEK"
     gene            397..693
                     /locus_tag="WM704_002146"
     CDS             397..693
                     /locus_tag="WM704_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341470.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPV
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            690..929
                     /locus_tag="WM704_002147"
     CDS             690..929
                     /locus_tag="WM704_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341471.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT          364 a          331 c          244 g          271 t
ORIGIN      
        1 aaaaccgcac ggaacatccg ttttcccgtt tccacgacaa tccggtacag gacaccccgc
       61 tgccgattcc gcctgcacat ccggcaaaat ttcatacgca aggaaaatgt tgaaaacaaa
      121 aagaactaat tgcttgaaaa aagatttaat tggaatgcgg gtatttccca cactttctcc
      181 atttatttat cctcattata aaaacatgaa aaaaaccctg cttgcactct cacttgcagc
      241 cgtgtccgcc gttacggtag ccgatattcc cgagtctaac ggcaaaggcg aatacaaagt
      301 ctttaccgct tatgttaaag gcaaagccac tgccggcaat ttgaaagaaa aataacgcac
      361 accgtccatc ggttttcgtt caacgaacgc cacgatatgc gttttaaggc agagtacgcc
      421 cgaagcaaaa acgatgcaga cgacaaaaga gggaatgcac acatcgctaa cggcgcaata
      481 ggcaaaatgg ctgccgtgta tgccggttat acctacaccc aaccggtcgg cgaatccaca
      541 cgtttccgtg gcggtttgga gcgtgtccaa gttaaacgtt atctgggaga aaaatgctgc
      601 ggctacgcct gtccaaaaag cgaacgcttc accctgcaag cagatggtga aatccgtcaa
      661 aaccttggcg aaggctggtt tctgaagcta tgagcggggt ggctgtctat ctccgcaaca
      721 aaaacaaaat agcaattgcc tcccaagatg ccaacctaaa ctccaaaggc cggttcgtca
      781 gcagcggtct gaatgtgggc aaacaactga ccggcagtct cggcgtagag tttgacccat
      841 actaccgcca ccgcgcaatc cgcaagtctg ccgaatttgt cccgaacact accaaaacca
      901 aaacagacag cgaaaagttt aacgagtagg gcttccgcgt agccgcaacg ttctaattcc
      961 cttacgtata aaaagcagca taaaaatgct gccttttccc atttccaagg gaagtattcc
     1021 gtagtgcagc ttacctcatt gtttcacaca aaccgaaaat gccgtcttaa aaccaatttt
     1081 cagacggcat ttgtccctta aaacacgttt tttcaagcgc gaccatacca aacacccaca
     1141 ggttgccttc cacccttcgt acaacctttg ctccatcaaa catccacttc ctaaatactg
     1201 atgtatactg
//
LOCUS       DAWTHD010000190         1210 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWTHD010000190 DAWTHD010000000
VERSION     DAWTHD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.190"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            293..1090
                     /gene="truA"
                     /locus_tag="WM704_002148"
     CDS             293..1090
                     /gene="truA"
                     /locus_tag="WM704_002148"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFA9341472.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQTA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGPMRRAAALLVGEQDFS
                     SFRAAGCQAKSPVKTIYRTGLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
BASE COUNT          275 a          402 c          305 g          228 t
ORIGIN      
        1 ccgattcccg ccgccgtcat tcccgcgcag gcgggaatct ggttttgtcg gtgcggaaac
       61 ttatcggata aaatggtttc tttagatttt tacgtcctag attcccgcct gcgcgggaat
      121 gacggttcgg gcactcctga cagggtgaat tcacaggata gcgattcgta gcaactgcat
      181 ccaacccccc gaacaactcc ccaaacaacg ctgcgcgccc cgggcgtttg ccgtttccct
      241 gcaaaatctg cgatacaatg cagtctgaac atttatccga atcccaaacc cgatggatac
      301 cgcacaaaaa caacgctggg caataaccct atcctacgac ggcagccgct tttacggctg
      361 gcagaaacag gcgggcggcg taccgaccgt tcaggcggca ttggaaaccg cactcgcccg
      421 aatcgcagga gaatctgtcg ccaccacagt tgccggcagg accgacaccg gcgtgcacgc
      481 tgccgcccaa gtcgtccact ttgacacaac tgccgcccgt ccccaacagg catggatacg
      541 cggcgtaaat gcccacctgc ccgaaggcat tgccgttttg cacgcccggc agaccgcacc
      601 cggatttcac gcacgcttcg acgcatacgg acggcactac cgctacctgc tcgaatccgc
      661 ccccgtccgt tcgccgctgt tgaaaaacag ggcgggctgg acacacctca aactcgacat
      721 cggaccgatg cgccgggctg ccgccttatt ggtcggcgaa caagacttct ccagcttccg
      781 cgccgccggg tgccaggcaa aatcccccgt caaaaccatc taccgcaccg ggcttaccca
      841 aagcgcagga ctgatccgcc tcgatttgca cggcaacgcc tttttgcacc acatggtacg
      901 caacatcatg ggcgcgctcg tttatgtcgg cagcggcagg ctcagcgtcg aaggcttcgc
      961 cgcactgatt caagaacgca gccgcctcaa agccccaccg accttcatgc ccgacgggct
     1021 ttacctgacc ggcgtcgact atcccggggc atacggcatc gtccgccccc aaatccccga
     1081 atggctttaa aacattcttg tcgcggagat tttgaaatcg gacaaactgt caggcaatct
     1141 ttttcaatgt tgacactata gtggattaac aaaaatcagg acaaggcggc gggccgcaga
     1201 cggtacaaat
//
LOCUS       DAWTHD010000191         1174 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWTHD010000191 DAWTHD010000000
VERSION     DAWTHD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.191"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            115..876
                     /locus_tag="WM704_002149"
     CDS             115..876
                     /locus_tag="WM704_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFA9341473.1"
                     /translation="MYIVKSDYSKDAKAPRVQVLFAEDYLSISLCDLWTNINTTGLEA
                     EGNVELKNGKKPESLIETIIKLATNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMD
                     YIETLAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETAKQQILACENSDGI
                     KTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLSE
                     MDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            866..>1174
                     /locus_tag="WM704_002150"
     CDS             866..>1174
                     /locus_tag="WM704_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFA9341474.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMIAALILYYFEKGYRH
                     F"
BASE COUNT          418 a          201 c          243 g          312 t
ORIGIN      
        1 gcgtatagaa ttgtaagaac agcagctagt agtagcgtaa aaaaattagc agatgaaaaa
       61 aaagaaattt gccaacaaca atttttttca gttatttcta aacgagataa attattgtat
      121 atcgtcaagt cagattactc aaaagatgca aaagcaccac gagtgcaggt tttgtttgca
      181 gaagattatt tgagtattag tttatgcgat ttatggacaa atataaatac cacaggacta
      241 gaagcagaag gaaatgtaga gttaaaaaat ggtaaaaagc cagaaagttt aattgaaacc
      301 atcattaaac tagcaaccaa cgaaaacgac atcgttctag actaccactt gggcagtggc
      361 acaaccgccg ccgtcgccca taaaatgaac cgccaataca tcggcattga acaaatggat
      421 tatattgaaa cgcttgccgt tgaacgcatg aaaaaagtaa ttgacggcga gcaaggcggt
      481 atctcaaaag ctgtgaattg gcaaggtggc ggcgagtttg tttatgccga actttcccca
      541 tttaacgaaa ccgcaaaaca acaaattttg gcgtgcgaaa attcagacgg catcaaaacg
      601 ctgtttgaag gtttatgcga acgccatttc ttgaaataca acgtcagcgt aaatgaattt
      661 agccaaatta ttgaagagcc tgaatttcaa tccttggcat tagacgaaca aaaacaaatg
      721 atgcttgaaa tgttggattt aaatcaaatg tatatttcat tatccgaaat ggacgatgaa
      781 caatttgcag gctgcctgaa tgatgatgat aaagcgttaa gccgtgcgtt ctatcaatca
      841 gtaaaacatc aagcggagaa aaaagatggc gaataataaa acgttgtttg attgggtgga
      901 agaccgcaaa tcaatgctgg aagaaatgga acagacggat tttttcgcgc tgcctgaatt
      961 tgtttccaat aatttaaaat atccgttttt tgaatggcaa aaatcggctt tagaaaattt
     1021 tgtgattttt gaccgcactt caaaattaaa ggatttcccc gacatcaaaa accgccctac
     1081 ccatttgctg ttcaatatgg caacaggtgc aggcaaaacg atgatgatag cggcattgat
     1141 tctgtattat tttgagaaag ggtatcggca tttt
//
LOCUS       DAWTHD010000192         1165 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWTHD010000192 DAWTHD010000000
VERSION     DAWTHD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.192"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..795
                     /gene="mafB"
                     /locus_tag="WM704_002151"
     CDS             <1..795
                     /gene="mafB"
                     /locus_tag="WM704_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9341475.1"
                     /translation="TDTAAQQTLQGINDLGNLSPEAQLAAASLLQDSAFAVKDGINSA
                     RQWADAHPNITATAQTALAVAEAAGTVWRGKKVELNPAKWDWVKNTGYETTVTRKTLD
                     GQIAGKNKPIKPSSGDYVLPVGRQIIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNE
                     KKSWVGDRVDVVNMPDGAPTSMDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKH
                     DGIEPQTWGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            792..1163
                     /locus_tag="WM704_002152"
     CDS             792..1163
                     /locus_tag="WM704_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFA9341476.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          400 a          208 c          250 g          307 t
ORIGIN      
        1 acagatacag ccgctcagca gactctacaa ggtattaatg atttaggaaa tttaagtccg
       61 gaagcacaac ttgctgccgc gagcctatta caggacagtg cttttgcggt aaaagacggc
      121 atcaattccg ccagacaatg ggctgatgcc catccgaata taacagcaac agcccaaact
      181 gcccttgccg tagcagaggc cgcaggtacg gtttggcgcg gtaaaaaagt agaacttaac
      241 ccggccaaat gggattgggt taaaaatacc ggctatgaaa caactgttac ccgaaagact
      301 ttagatgggc aaatagctgg caagaacaag cctattaaac cttcttcagg agattatgtc
      361 ttgccggtag gcagacaaat catagatcct actcgtacat ctttttcaca agctacagtt
      421 agctatcaga agcgtggtgc aaattacaac tatgattctc ttgtagctgc aatgaacgaa
      481 aaaaaatcat gggttggcga cagggttgat gttgttaata tgcctgatgg agcacctact
      541 agtatggata acacgcgtat tatggcagca cgtgaagcag gggtaaaagt ggaagcgaat
      601 gttcataatt ttaatgaccg attatcatca aaagagagaa tcaggtttaa gcatgatggt
      661 attgagcctc aaacttgggg agaagctatc cagctacgaa ttagaaagca agaaacacaa
      721 aaaggagttc cagaagggtg gagcaaaaga tttcctaacg gaagtattta tgatgtaaag
      781 gtacttagga aatgataaaa caaaatagtt ttgttccgta tcctgaagca atgcttccta
      841 aaggatttaa atatccgcaa agttatttaa aattagctca atccactcat gccattaact
      901 acgatgaaca atattctttt ccttggtggt ttgaaaatgc agaaagcaat atatcagaag
      961 taattgacat ttattttgaa ataactggca ttccaaacct attacctttt gctagaaacc
     1021 aagagtgggc tgcctgtttt gatatttcag ataaatcagg taatcctaaa attatagtag
     1081 ttaatttaga taatacaaaa tattacgaga cttttgaaaa ttttgatact tggctaaaag
     1141 aagctgaaaa tgatggttgg tagca
//
LOCUS       DAWTHD010000193         1129 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWTHD010000193 DAWTHD010000000
VERSION     DAWTHD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.193"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(84..>1129)
                     /gene="mafB"
                     /locus_tag="WM704_002153"
     CDS             complement(84..>1129)
                     /gene="mafB"
                     /locus_tag="WM704_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9341477.1"
                     /translation="VDGGFTVYQLHRTGSEIHPADGYDGPQGGGYPEPQGARDIYSYH
                     IKGTSTKTKINTVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWENNPNQNWWGNRM
                     DDVRGIIQGAVNPFLTGFQGVGIGAITDSAVSPVTDTAAQQTLQGINDLGNLSPEAQL
                     AAASLLQDSAFAVKDGINSARQWADAHPNITATAQTALAVAEAAGTVWRGKKVELNPT
                     KWDWVKNTGYKKPAARHMQTVDGEMAGGNRPLESKNTVTTNNFFENTGYTEKVLRQAS
                     NGDYHGFPQSVDAFSENGTVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKIN
                     HRLFVPNQQLPEK"
BASE COUNT          270 a          272 c          214 g          373 t
ORIGIN      
        1 tctctaattc caattcctat caaagattcc ccttgtctcg aataaatttc aaaaatttca
       61 tttaacatat taatctctat ctactatttt tcaggaagtt gttggttagg gacaaataat
      121 ctatgattaa tttttccatc tgcttctctg atatattcaa aatttccatc ttttccttta
      181 taagatccag ggatataaag tttatgcctt acaatattat ctccgccaac tatttgtatt
      241 actgttccat tttcagaaaa agcatctact gattgcggaa aaccatgata atcaccattc
      301 gatgcttgcc ttaatacttt ctctgtataa ccggtatttt caaaaaaatt attcgttgtt
      361 acagtatttt ttgattccag cggtctattc ccccctgcca tctccccatc tacagtctgc
      421 atatggcggg cagcaggttt tttatagccg gtatttttaa cccaatccca tttggtcggg
      481 ttaagttcta cttttttacc tctccaaacc gtacctgcgg cctctgctac ggcaagggca
      541 gtttgggctg ttgcagttat attcggatgg gcatcagccc attgtctggc ggaattgatg
      601 ccgtctttta ccgcaaaggc actgtcctgt aataggctcg cggcggcaag ttgtgcttcc
      661 ggacttaaat ttcctaaatc attaatacct tgtagagtct gctgagcggc tgtatctgtg
      721 accgggctta ccgcactgtc tgtaattgcc ccaatcccta ccccttgaaa acccgttaaa
      781 aaaggattaa ccgcaccttg gatgatgccg cgaacatcat ccatacggtt accccaccaa
      841 ttttgattgg ggttgttttc ccatatcagt tttcctgctt catcagcacg gctgaaaaaa
      901 ccggaagcgg caccggcatt ttcttttagc cagcggtctg aaaaaggggc ttgcggaaca
      961 gtgtttatct ttgtttttgt agaagttcct ttgatatggt aactgtatat atcccttgcc
     1021 ccttgtggtt ccggataacc gccgccttga ggcccgtcat atccgtctgc gggatgtatt
     1081 tccgaccctg tccgatgaag ttggtaaacg gtaaaaccgc cgtctacgc
//
LOCUS       DAWTHD010000194         1127 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWTHD010000194 DAWTHD010000000
VERSION     DAWTHD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.194"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..277)
                     /locus_tag="WM704_002154"
     CDS             complement(<1..277)
                     /locus_tag="WM704_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341478.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKKIGSV"
     gene            complement(314..577)
                     /locus_tag="WM704_002155"
     CDS             complement(314..577)
                     /locus_tag="WM704_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341479.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(597..845)
                     /locus_tag="WM704_002156"
     CDS             complement(597..845)
                     /locus_tag="WM704_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341480.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            complement(806..>1127)
                     /locus_tag="WM704_002157"
     CDS             complement(806..>1127)
                     /locus_tag="WM704_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFA9341481.1"
                     /translation="SARWQDIDLTARLWHIPAADMKTKHPFTVPLSDWAVEILRELHT
                     QTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTPANPNTPCTDSATSS
                     PTSA"
BASE COUNT          173 a          236 c          403 g          315 t
ORIGIN      
        1 gtacagaccc tatttttttg actgcccggc ggcaggtttc ggtaaggacg gcggcaaatc
       61 gggcttcgtc tgccggtttg ccgccgaaaa aaataaaatc gtaaagggtg atgccgtttt
      121 cggaatggcg gtaatgcccg cccgggcatt cgccgtctgt ctgcctgtgg attttggcaa
      181 tcaggcgggg atagcggcaa tgggtaatga aacggcttgc gccgtcttgc ccgataatcc
      241 attcggggta gcggttgaat agtgcggctc tgttcatttt gttgagggat aaagcccctc
      301 gcggggcttg tggtcaggca aatttgaata tcagtgccaa cacggcggcg atggcggtaa
      361 ccagaccggt ggccgccatc atgggatacc agcgggactc ttgggctatt tttacggatt
      421 cggcgttgat tttgtgcgcg tcggcaatga ttttggcgat ttcggcatct atttttctta
      481 actcggtgcg ttttgtttct tgttcgatta tgtttattgg atttcctttc gtttttggcg
      541 gttgccgccg cttgtcggat ggcaggatgt ctgccatgta tgtatgttaa taccttttag
      601 gttttattgc aagtgttttg ggcggcggct tcgtatgctt ggcggtggcg gcggccgtac
      661 cattcggcga gttcgcgccg ctcttggagg cggtagctgt cggcggtcag gtagtggtgc
      721 aggcttgaga agccggcgcg ttggagggcg gcgcggctga tgtggccaag ggcgaggtct
      781 gttgtgaggg tgtcttttcc ggcggtcagg ctgatgttgg tgaagaggtc gcggaatccg
      841 tgcatggtgt gtttggattt gccggggtgc tgccgtcata gcccagtcgt cggatggcgt
      901 tgtgggcgaa tttgatgctg atgtggtcgg gatggggggc gggtttgcgc cgtgggcgga
      961 tgcctgggaa aaggtggatg ttgtcgccgg tctgtgtgtg cagctctcgg agtatttcta
     1021 ctgcccagtc cgacagtggg acggtaaagg ggtgtttggt cttcatgtcg gcggcgggga
     1081 tgtgccataa ccgggcggtg aggtcgatgt cttgccagcg ggcggaa
//
LOCUS       DAWTHD010000195         1122 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWTHD010000195 DAWTHD010000000
VERSION     DAWTHD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.195"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(235..>1122)
                     /locus_tag="WM704_002158"
     CDS             complement(235..>1122)
                     /locus_tag="WM704_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341482.1"
                     /translation="MSAPLRDVIPEGAVLIVGEAHYTYPVRAAGRPVPPYIQELTELR
                     HHGHTVILMTRHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLDNPAGVSGV
                     EAANWKPPKEAFKYYKSSSRHQKFKKKVPWAVWALIAVVGFVGWKSYGMFQVYSKATD
                     SRIEQEAQKESVVQTMTEQTASSETAPFEHSDNLKPEDFVPTLPEKPESKPIYNTVRQ
                     VKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYKDERQRTEQ
                     AAQSAKADKPQVLVMGGKS"
BASE COUNT          243 a          272 c          257 g          350 t
ORIGIN      
        1 cataggctcg aaataaaaaa ctttcgacgt gtccgggaac ttatggagac ggagaacacg
       61 tgtgaaatcc ccgttttcat ctacaatttt gatatatctt ggtttgtgaa tcatgacatc
      121 ctcagattta gtattcagaa tatgatttta aaaagaactt tctgctttac gactccgccg
      181 ccgattcctt caaacggttt tccgcgctct tcagttgtcg tacattaaat tttattagga
      241 ctttccgccc attacgagaa cttggggctt gtccgctttc gcggactgtg ccgcctgttc
      301 cgtcctttgc cgttcgtcct tgtaaggatt gaaaggcaac ccgtttttca catattcttt
      361 acacattatc tttgttattt ctttcaaggg tgttccttga tttgaatagc atgtgcaatc
      421 tgattttccg ccgtctatgc atccggcgat ttgctcaaag gtttttactt gtcggactgt
      481 gttataaata ggcttgcttt cgggcttttc gggcaaagtc ggcacaaagt cttcaggttt
      541 cagattgtcg gaatgctcaa aaggcgctgt ttctgatgat gccgtctgct ccgtcatcgt
      601 ctgcacaacg ctttcttttt gcgcttcctg ctcaatccgg ctgtctgtgg ctttgctgta
      661 aacttgaaac atgccgtaac ttttccagcc tacaaaccct acaaccgcaa tcaacgccca
      721 aaccgcccaa ggcacttttt tcttgaactt ttggtgccgg cttgatgatt tatagtattt
      781 gaaggcttct ttaggcggtt tccaatttgc ggcttctacg ccgcttacgc ccgcgggatt
      841 gtccaacgag gttacgcatt tataccaata atactgtttc atgccgattg ccttgcgttc
      901 aaggtgtaca tgctttgaaa caaggttgcg gacgaatata tcaagttggc tcgggtgccg
      961 cgtcatcaaa atgacggtat gcccgtgatg gcggagttct gtcagttcct gaatataggg
     1021 cggaacggga cggcctgccg cgcgtaccgg gtaagtgtag tgcgcttcgc caacaatcag
     1081 caccgcgcct tccggtatga catcacgaag cggggcggac at
//
LOCUS       DAWTHD010000196         1011 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWTHD010000196 DAWTHD010000000
VERSION     DAWTHD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.196"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
BASE COUNT          299 a          157 c          248 g          307 t
ORIGIN      
        1 caggggaacc tgtcggggag acgcttaaaa ttgagcaaat gattgatttc tttttagtct
       61 aaatcataca taccagtgga caagctaggc gtagatccgg cttttaaata cgattgtcag
      121 gtttatactt gggataaaat caaaaataaa gttgtttaaa ctatagtgga ttaacaaaaa
      181 tcagggcaag gcgacgaagc cgcagacagt acagatagta cggaaccgat tcactcggtg
      241 cttcagcacc ttagggaatc gttccctttg agccggggcg gggcaacgcc gtactggttt
      301 ttgttaattc actatattgc atggtgcatg cattaaatca gtcttctagc gccgcaatgg
      361 gaaaacatag aagataaacg aaatggatga tctaatacta tattttttat caggtatatt
      421 cggaaatcaa gttgctgaat atattataaa aaacaaccgc gagataaaag ttccctttat
      481 tgcgctttac gctatatttt ttacgttgat ttatactgtg gctttgttgt ttctcagtct
      541 gatctattgg gtcaatggtg cggagattgc atggaagggg ataggtattt tcagtatgtc
      601 ggtcagtttt tgtatagtct tctgtcttta tttggttgac aaggcaggaa gatgtaagga
      661 taagaaacaa tagggatcag atgcggcagc aaacgtagat tgggtctagg ctcaacctgc
      721 gtttgctgca tctttggaag aaatagtgga ttcgaaaaaa taggaagaat tctgattaat
      781 ggtatgcgag gtgtagcagt gggggctgtt gctggtggca taactggtta tgcaggttct
      841 acgggtaaga acactgacat tcgccgttaa ataataaata aaccaacttc caaccaaacc
      901 tgctatcggt ttggttggaa gtgagaggag gtaaggtagt gtttgctgtt attttctttt
      961 cgacattggg ctgtatttta gcatggataa gagatattcc taaaataaaa t
//
LOCUS       DAWTHD010000197          996 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWTHD010000197 DAWTHD010000000
VERSION     DAWTHD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.197"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..576
                     /locus_tag="WM704_002159"
     CDS             <1..576
                     /locus_tag="WM704_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFA9341483.1"
                     /translation="LTPASAEAPHAQPPPEVSPAENPANNPDPDENPGTRPNPEPDPD
                     LNPDANPDTDGQPGTSPDSPAVPDRPNGRHRKERKEGEDGGLSCDYFPEILACQEMGK
                     PSDRMFHDISIPQVTDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYRASYEPLCVFAEK
                     IRFAVLLAFIIMSAFVVFGSLGGNKCHYLPA"
     gene            555..845
                     /locus_tag="WM704_002160"
     CDS             555..845
                     /locus_tag="WM704_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFA9341484.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLISGFGQGLGCLFGAFSFFIGMHAFKKLTFVFPG"
BASE COUNT          249 a          267 c          245 g          235 t
ORIGIN      
        1 ctcacgcccg caagcgcgga agcacctcac gcacagccgc cgcccgaagt atcgcccgcg
       61 gaaaaccccg caaacaaccc ggaccccgat gagaaccccg gcacgcgtcc caatcccgaa
      121 cccgatcccg atttgaatcc cgatgcaaat cccgatacgg acggacagcc cggaacaagc
      181 cccgattccc cggccgttcc ggaccgccca aacggcaggc atcgcaaaga aaggaaagaa
      241 ggcgaagacg gcgggctttc gtgcgattat tttccggaaa tcctagcctg tcaggagatg
      301 ggcaaacctt cggaccgcat gtttcacgat ataagcatac cgcaggttac agacgataaa
      361 acatggtctt cacataactt tttaccgtct aacggcgtat gtccgcagcc gaaaaccttt
      421 catgttttcg gcaggcaata tcgggcaagc tatgaaccgt tgtgcgtgtt tgccgaaaaa
      481 atccgttttg ccgtactgct cgcctttatc attatgtcgg cttttgtcgt tttcggttcg
      541 ttggggggga ataaatgcca ttacttgccg gcctgattcc acttttaggc atacttctga
      601 aaatgctgat tgtcagaatc atccttgcaa caggtctgac attcgtaacc tatgccgggt
      661 atctcgccgc actggaaaag ttcaaaggct acacggcaaa tgcgatcaat tccatgcctt
      721 ccgacatatt gaaccttctt ttaatttcgg gattcggtca ggggttgggc tgcctgttcg
      781 gcgcattctc gttcttcatt ggtatgcacg cattcaaaaa actgacgttt gtctttccgg
      841 gatgaggtag aagcatgatt tatctgttta cgggaaacat gggggcaggc aaaaccctat
      901 gattttgaac aacgaagacg gattgttcaa aatgaaattg gaagacggca cggaggcaga
      961 ccggccgctt tatttctgcc atatcgacgg attgga
//
LOCUS       DAWTHD010000198          953 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWTHD010000198 DAWTHD010000000
VERSION     DAWTHD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.198"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(<1..254)
                     /locus_tag="WM704_002161"
     CDS             complement(<1..254)
                     /locus_tag="WM704_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341485.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNT"
     gene            complement(257..445)
                     /locus_tag="WM704_002162"
     CDS             complement(257..445)
                     /locus_tag="WM704_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFA9341486.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(513..851)
                     /locus_tag="WM704_002163"
                     /pseudo
     CDS             complement(513..851)
                     /locus_tag="WM704_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          235 a          193 c          173 g          352 t
ORIGIN      
        1 tgtgtatttg tataaaattt ttcgttggta tctgcttgat attttaattg tctcaatatt
       61 tccatgattt catattctga tttttcatca tgttcttcaa tcatgtaatc aacaaattgt
      121 tttagaatat caaaggcttc gtaactttcc aaaattttaa tatgtttatc ggcaaaattg
      181 gacatatacc attcgtcagt gattggattg gtatttaaat catctaaaaa aacatttaca
      241 tcatctaaaa acatggctaa tccttaaact tgaaatctgt tttctcaccg gttcttgtat
      301 tttcaatgta atgtatctca actttgttta tattttgaac gcgcttaacc cagccgtctt
      361 ttgccaacca accattttta gtatcggaca taggaggtat tctcgcagga gttttgccct
      421 gtgggtttgc cttaacttct tccattgcta attgttcctt taaattatta ggtattgtac
      481 gaccggttga tttttcaaca gattgtgtcc ctatttttaa tgatttgttt ccccccagcc
      541 atttccctgt ctacagtctg catatggcgg gcagtaggtt tttcatagcc ggtatcttta
      601 actcaatccc atttagtcgg gttaagttct acttttttac ctctccaaac cgtacctgcg
      661 gcctctgcta cggcaagggc agtttgggca gtagctgtaa tgttcggatg ggcatcagcc
      721 cattgtctgg cagagttgat accgtctttt accgcaaaag cactgtcctg taataggctc
      781 acggcggcaa gttgtgcttc cggacttaat tttcctgaat tattaatacc ttgtagagtc
      841 tgctgcgcag agatgtagca actgtatttt taccccgtcg ggtaaaaata cagttgctac
      901 ggctgctggg aaagcagtta ctattttccc agtggctcaa aattaattgt ttc
//
LOCUS       DAWTHD010000199          886 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWTHD010000199 DAWTHD010000000
VERSION     DAWTHD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.199"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            178..738
                     /locus_tag="WM704_002164"
     CDS             178..738
                     /locus_tag="WM704_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HFA9341487.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT          248 a          296 c          186 g          156 t
ORIGIN      
        1 aatctggtac agacccaaaa acaaagcacg gataacaccg cgctttttca tccgccctgt
       61 tccgcccgcc cgttcgtcaa ccgcctgcaa acccaccgtt taccggcagg aaatgccata
      121 aatccccgcc tatgttagga taaacgcctt taccggccca cacaaaggat tatcatcatg
      181 aaaaaaatca tcttcgccgc gctcgcagcg gcagccgtcg gcactgcctc cgccacctac
      241 aaagtggacg aatatcacgc caacgtccgt ttcgccatcg accacttcaa caccagcacc
      301 aacgtcggcg gtttttacgg tctgaccggt tccgtcgagt tcgatcaagc aaaacgcgac
      361 ggcaaaatcg acatcaccat tcccgtcgcc aacctgcaaa gcggttcgca acccttcacc
      421 ggccacctga aatccgccga catcttcgat gccgctcaat atccggacat ccgcttcgtt
      481 tccaccaaat tcaacttcaa cggcaaaaaa cttgtttccg ttgacggcaa cctgaccatg
      541 cgcggcaaaa ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg
      601 atggcggaaa ccgaagtttg cggcggcgac ttcagcacca ccatcgaccg caccaaatgg
      661 ggcgtggact acctcgttaa cgccggtatg accaaaaacg tccgcatcga catccaaatc
      721 gaagctgcaa aacaataagc cttgatgcga atcagcacaa atgccgtctt catcaaaccg
      781 ccctgccaac cgtaagccga tatggcggag cacaacccgc cgaaagatat tttggcggca
      841 ggcggggctt gccggaaagt ccggaaagta acgaccgaaa acaacg
//
LOCUS       DAWTHD010000200          819 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWTHD010000200 DAWTHD010000000
VERSION     DAWTHD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.200"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            complement(45..779)
                     /locus_tag="WM704_002165"
     CDS             complement(45..779)
                     /locus_tag="WM704_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9341488.1"
                     /translation="MRVERRQCAEHGGYAAKSVLRGVWTGCPACRKLEAADEMAAYAE
                     TLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMARAKAAAAEYAANFADV
                     LQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGDMLRTVKDSFGGGGGEA
                     GAVGVFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLMPMLVLTNLTAEAFREN
                     TDARIRDRLRDGGGKLIPFDWESYRA"
BASE COUNT          133 a          312 c          224 g          150 t
ORIGIN      
        1 ccggttgggt tttaaaatcc gcgtgtttgc agaaaaagca ggtttcacgc acggtagctc
       61 tcccagtcga acggaatcag cttgccgccg ccgtcccgca gcctgtccct gatccgcgcg
      121 tcggtgtttt cgcggaaggc ttccgccgtc aggttggtca gcaccagcat cggcatcagc
      181 cgctcgtacc gggcgttgac gacggaaaac aaaatccgcc cgtccgtttc cgacagactg
      241 cccgcgccga actcgtccag caccagcaaa tcgggcttca cgaaaacccc gaccgccccc
      301 gcctcgccgc cgccgccgcc gaaactgtcc ttgaccgtcc gcagcatatc gcccaccgtg
      361 atgaccagcg cgcttttgcc ggcggcgatg acttcgcggg cgatgccgca ggcaaggtgg
      421 tttttgcccg tgcccctcct gcccgaaaaa atcatgctcc gccccgtctg caacacatcg
      481 gcgaagtttg ccgcatactc ggcggcggcc gccttcgccc tggccatccc cgggatcgaa
      541 tcgctgacgg cgtagttttc aatccggcag tttctgaacc gcggggcgat gcccgaacgc
      601 ccgatgcgtt tttccagcgc gtcgcgcatc gccccgcggc gcagcgtttc cgcgtatgcc
      661 gccatttcgt ccgccgcctc cagcttccgg caggccgggc agcccgtcca cacgccgcgc
      721 aaaacgcttt tcgccgcgta tccgccgtgt tccgcgcatt gcctccgctc gacccgcacg
      781 ccgccgccgt aagcccccaa gaaatcagac gcgttcctc
//
LOCUS       DAWTHD010000201          815 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWTHD010000201 DAWTHD010000000
VERSION     DAWTHD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.201"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
BASE COUNT          244 a          167 c          148 g          256 t
ORIGIN      
        1 aagaaactcc taatggcgga gtggggcttt tgtttaagta agcattgaaa aaatttaaat
       61 ttcccattat gattaactta atgcaagagc tttttcagga cgtacaaacc agcgaatgat
      121 gtgggcaggt tggttgtcgc taaccaattt cagtagctca atcgtcagat tggtatccga
      181 ccctatcaag ctaaggagag gctgcggcta ctacacaaag cacctgaaat tattgggtgc
      241 ttttgtcttt ttatggcatt gtaccaattt gtgtaccacg agggcttgtt cgcccaaacg
      301 cggtaacaac tgaatacaca ctttccccct caacttcttt gcgttctaaa acgactgtcc
      361 caatccgaga atagacaatc ataggacgat gattgcccct tacatcacta aattcactaa
      421 aaatttttgc gcctgattga aaaatcattt ccacataaaa gggttacacc tgtttcatcg
      481 tggcttaatc caaaccgcgt taaatctgct ttgtgttctg ccaaaatgtg aatcacacca
      541 aagccgtgat taaccttttt gtgttcgccg tgttttaaaa caatttgacc tgccgggaag
      601 tctgctctat tgttcggaca ataaggggaa tataccaatt actttaacta catcattttt
      661 tgacattttt cttgcctttc aatttaaaat ccaaaatcat actgccataa tttagcaatc
      721 caaaaaaatt taggcagcag taatggtttc gtattttaga aaacgaaact tggtttggtg
      781 ttttgaagca tttcagtaca caaacatgga atttt
//
LOCUS       DAWTHD010000202          803 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWTHD010000202 DAWTHD010000000
VERSION     DAWTHD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.202"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..101
                     /locus_tag="WM704_002166"
     CDS             <1..101
                     /locus_tag="WM704_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFA9341489.1"
                     /translation="KPFPPLLRECEFRLNSGTPSRQLKILRDRCGI"
     gene            complement(126..755)
                     /locus_tag="WM704_002167"
     CDS             complement(126..755)
                     /locus_tag="WM704_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFA9341490.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT          162 a          198 c          218 g          225 t
ORIGIN      
        1 gtaaaccttt cccgccgttg ttgagggaat gcgaatttcg acttaactcc ggcacaccgt
       61 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagccc
      121 catttttaat gtctgtgcgg catcatgcct tcgggggctt ctgttccttc cggcaaaccg
      181 aaggtttcac gcagaatcac tttatagaaa gcaaacgctt cctgcgcgcc ttgaatggct
      241 tctgcttctg cttccggagt taagttcagg gcattcaggt gctcgacgaa ggcgcgccag
      301 tgtttgccgc gcccgtccgg atgcggagcg aggtggcgcg cgccgtgttc gccggtgtaa
      361 tccagctttt gcgcgtgttt gaacaaaaat gccgcgccca aattggatcc ttcggcgcaa
      421 taaagccagc cgacggcttt gttgccggtt tcgtgtggca attctttgtc gaatttgtaa
      481 ggctcttcgc ccaagtcttt caagtcttgc gttacggcat cgtatcgcgc catgtattcc
      541 agctcggaaa tggctttgtt caactcgggg tctttataga tatgatcaac cgctttgtgg
      601 aacacggatt gcagtttcaa aaatttgatg tagttttctt tgctgacaaa cggttggaca
      661 gacataacga ggttatccac gctgtcgtga accgccgtgg tatccgcctt caggcgtttg
      721 gcaaacgtca gggcttggtt ttcggtttcg ctcataagtt ctcctttgtc ggtaagggat
      781 aaggatgcgg tgcgggcaaa atc
//
LOCUS       DAWTHD010000203          783 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWTHD010000203 DAWTHD010000000
VERSION     DAWTHD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.204"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            429..>783
                     /locus_tag="WM704_002168"
     CDS             429..>783
                     /locus_tag="WM704_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9341491.1"
                     /translation="MRNAVGLDISKLTFDASAMVGKTEHSAKFDNDSKGLDQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTGKQDAK
                     LIAQYCRSAQESELVK"
BASE COUNT          248 a          160 c          192 g          183 t
ORIGIN      
        1 tttttttggg cgtatctcag tccggaatcg cttcgttcgg gggttgtacg tgcaggaaaa
       61 tagggcggaa aaaaggaaaa gggggaagct ttgtaaagat tgggtgcgtt acccaatctt
      121 tacgaatacc cccttttcct tttttatgaa ctgtttttca ataccgcaaa cccactaacg
      181 gagtgattcc ggactgagat acgcctaaaa aaaatcagac attcgggtcg caacaggaat
      241 ctttaccaaa acctgcaacc caaataaaat cagacacggc aaaggaatag ctaccctttg
      301 ccgaaaccgt ccggcctgaa caaaccacaa acttaaagtt tgatgacgag aataggcggg
      361 cggttttctt gtttgtgaaa ttgagtagta tcaaagaaca cagattctga atatataagg
      421 gtaatcccat gcgtaacgcc gtaggattgg atatatccaa gctgacattt gacgcatccg
      481 ccatggtcgg caaaacggag cattcggcaa agtttgacaa cgattcaaaa ggtttagatc
      541 agttttcgga ccggttgaaa agcttgggat gtcagaatct gcatatctgc atggaggcaa
      601 cgggaaacta ttatgaagaa gttgccgact acttcgcgca gtattacagc gtttacgtag
      661 tgaacccgct gaaaataagc aagtatgcag aaagcaggtt caagcgaacc aaaacaggca
      721 aacaggatgc aaagctgata gcgcagtatt gccggtcggc gcaggaaagc gagcttgtaa
      781 aga
//
LOCUS       DAWTHD010000204          765 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWTHD010000204 DAWTHD010000000
VERSION     DAWTHD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.205"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..726
                     /locus_tag="WM704_002169"
     CDS             <1..726
                     /locus_tag="WM704_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFA9341492.1"
                     /translation="TYAAARKTLQGIHNLGNLSPEAQLAAASLLQDSAFAVKDGINSA
                     RQWADAHPNITATAQTALAVAEAAGTVWGGKKVELNPAKWDWVKNTGYKKPAARHMQT
                     VDGEMAGGNRPPKSITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGKKN
                     FPIGTATYEEADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQAN
                     FETYTIDSNEKRNKIKNGHLNIR"
BASE COUNT          277 a          137 c          175 g          176 t
ORIGIN      
        1 acctatgcgg cagcacggaa aactttacag ggtattcaca atttaggaaa tttaagtccg
       61 gaagcacaac ttgccgccgc gagcctatta caggacagtg cttttgcggt aaaagacggc
      121 atcaattccg ccagacaatg ggctgatgcc catccgaata taacagcaac agcccaaact
      181 gcccttgccg tagcagaggc tgcaggtacg gtttggggcg gtaaaaaagt agaacttaac
      241 ccggccaaat gggattgggt taaaaatacc ggctataaaa aacctgctgc ccgccatatg
      301 cagactgtag atggggagat ggcagggggg aatagaccgc ctaaatctat aacgtcggaa
      361 ggaaaagcta atgctgcaac ctatcctaag ttggttaatc agctaaatga gcaaaactta
      421 aataacattg cggctcaaga tccaagattg agtctagcta ttcatgaggg taaaaaaaat
      481 tttccaatag gaactgcaac ttatgaagag gcagatagac taggtaaaat ttgggttggt
      541 gagggtgcaa gacaaactag tggaggcgga tggttaagta gagatggcac tcgacaatat
      601 cggccaccaa cagaaaaaaa atcacaattt gcaactacag gtattcaagc aaattttgaa
      661 acttatacta ttgattcaaa tgaaaaaaga aataaaatta aaaatggaca tttaaatatt
      721 aggtaattat atgagattaa aattaaaaaa ttatgattgt tctga
//
LOCUS       DAWTHD010000205          723 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWTHD010000205 DAWTHD010000000
VERSION     DAWTHD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.206"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            68..>723
                     /locus_tag="WM704_002170"
     CDS             68..>723
                     /locus_tag="WM704_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9341493.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDDGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEPLDYEPSDYEPSDFEPSDYEPSDYEPSDFDAKNHQILSGEPSDFALRTVR
                     FCAKNHQILSDEPSDFDGSLYVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPET
                     A"
BASE COUNT          184 a          179 c          219 g          141 t
ORIGIN      
        1 gcatgaaggc gggcggccgc gggaagcctg ttacgcatta ttcgattgtc aaaaacggag
       61 cggaagtatg agtgcgaggc tgatggggat ggctttcaaa acgggtatcc cgagggggca
      121 gcgttttgtt ttggtaaagt tgtgcgactg cgccaacgac gatggcttgt gttatccgtc
      181 gcaagaaacg ctggcggaag atacgggctt cgccgaaacc gccgtacggc agcatatcaa
      241 gtggctgaag gataacaatt tcatcaagtc cgcccggcgg cagagggggc gggagaggaa
      301 gtccgacatc taccgcatca acgtcgccct gcttgaaaaa tgctatgcgg aggcggcaaa
      361 acggaaggcg gcgcggcagg caaaaatgtg ggaagaacca ttggattacg aaccgtcgga
      421 ttacgaaccg tcggattttg aaccttcgga ttacgaaccg tcggattacg aaccgtcgga
      481 ttttgacgct aagaaccatc agattttgag cggcgaaccg tcggattttg cgctaagaac
      541 cgtcagattt tgcgctaaga accatcagat tttgagcgat gaaccatcag attttgacgg
      601 ttccttatat gtagaaccgt cagtagaacc gtcagtagaa ccgtcaggat caaatgcgcg
      661 cggcgcgcgc gcccctgccg gaccgcaccc tgcgaaaccg caaacggcgc ctcccgaaac
      721 cgc
//
LOCUS       DAWTHD010000206          696 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWTHD010000206 DAWTHD010000000
VERSION     DAWTHD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.207"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
BASE COUNT          157 a          174 c          175 g          190 t
ORIGIN      
        1 atttgtactg cctgcggccc gccgccttgt cctgattttt gttaatccgc tataaagacc
       61 gtcgggcatc tgcagccgtc attcccgcgc gggcgggagt ccggacctta gaacaacagc
      121 aatattcaaa gattatctga aagtccggga ttctggattc ccgctttcgc gggaatgacg
      181 gttcggttgc tacggttact gtcaggtttc ggttatgttg gaatttcggg aaacttatga
      241 atcgtcattc ccgcgcaggc gggaatctag gccggtcggt gcggaaactt atcgggaaaa
      301 aaggtttctt gagattttac gtcttggatt cccacttttg tgggaatgac gtggtgcagg
      361 tttccgtacg gatggattcg tcattcccgc gcaggcggga atccggtccg ttcggtttca
      421 gttatttccg ataaattcct gctgcttttt atttctagat tcccacttcc gtgggaatga
      481 cggcggaggg gataagttct tgcaatctaa aatttcgtca tttctataaa atagcaaacc
      541 gaaacagaaa cttaaaaaca gcaacctgaa acagcaacct gaaaccccgt cattcccgcg
      601 caggcggaaa tccggcgggc cgtagggtgt gcttcggccc gccatttcca tcaatccaac
      661 atgtctaccg ttttcatcga atccatcgaa tccgcc
//
LOCUS       DAWTHD010000207          637 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWTHD010000207 DAWTHD010000000
VERSION     DAWTHD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.208"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            244..585
                     /locus_tag="WM704_002171"
     CDS             244..585
                     /locus_tag="WM704_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341494.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVTAVCLG"
BASE COUNT          173 a          147 c          178 g          139 t
ORIGIN      
        1 tagggaggga tgtcgtgata tttgaaaaat caataaacgg ctggaaatac cgcgccgtaa
       61 tggaaatcag aaccacgcaa aaggagcttg ccttgaacag cctttataca acagacagga
      121 ttgaatagtc atgacaaaac ctgcgacgag taactgtcaa tctttcgatt ccgtcctcat
      181 acatcctaag cgtctttgca aattctgttc agttatacca tttatacaga caggagcaaa
      241 ccaatgacga agttatacgc agaaatcgcc gaaatggagg cgcaggacga cggcacggtc
      301 aaagtttggg gttatgcctc aagcgaggcg gtcgattcgg acggcgaagt catcgcggcg
      361 gcggctatga aggcggcgat tcccgattat atgaagttcg gcgaggtgcg cgagatgcac
      421 ggcagcaacg cggcgggaac ggcgattgaa atcaacgtgg aagacgacgg cagaaccttt
      481 ttcggggcgc atatcgtcga tcccgttgca gtggagaaag tcaaaacggg cgtttacaag
      541 ggcttttcca tcggcggcag cgttaccgct gtctgcttag gttgaatcgg cattcgtacg
      601 attacggcac accgcctaat ctgtacctgc gtctgtt
//
LOCUS       DAWTHD010000208          619 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWTHD010000208 DAWTHD010000000
VERSION     DAWTHD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.209"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            81..296
                     /locus_tag="WM704_002172"
     CDS             81..296
                     /locus_tag="WM704_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341495.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            407..>619
                     /locus_tag="WM704_002173"
     CDS             407..>619
                     /locus_tag="WM704_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9341496.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVKNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          230 a          107 c          158 g          124 t
ORIGIN      
        1 aagggcggaa gaatttgcag attagataaa ccgtagaaga tgttgacgac agcatcccat
       61 tttgagttaa ggagcaaaaa atgagctatt tggaagatgt aaaaaacgca ttaagggtaa
      121 tagataactt atgcaaagaa gcattaaaag agcctgaatc gttagagggt tatatagacg
      181 aaattaggga taaagcagac gaagcggata cctctttgga atttctaaag gatgtaataa
      241 attatggtat tagcgattta aaaaatgtaa ttgaggtgtt tgaagattgc gtttgatatt
      301 aatggaacaa gacatgaact atcaatttaa attcggcgac cccgtgagag atacagcttt
      361 atgattgggc attgagagaa cggcatcaaa aaatcgagaa aacaaaatgc aaacagtagc
      421 aacaaaaccg acggcaaaac agatgcttgc cgccaaacgc gcggcgaagg aatcaacgcg
      481 gcaggaacgc gccgtcaaac gcgcgggaac agtaaaaaac gttgaccgga accggctgtc
      541 cgcccggtca aaagcgcaaa aagaaaacat cgcccggatg ttgtcgggcg caaaggtatc
      601 ggaagacgaa gccctgacg
//
LOCUS       DAWTHD010000209          594 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWTHD010000209 DAWTHD010000000
VERSION     DAWTHD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.210"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..>594
                     /locus_tag="WM704_002174"
     CDS             <1..>594
                     /locus_tag="WM704_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9341497.1"
                     /translation="LLFSSLLFSSAARAASEDGGRGPYVQADLAYAAERITHDYPEPA
                     GAKKGKISTVSDYFRNIRTHSVHPRVSVGYDFGGWRIAADYARYRKWNNSKYSVSIKK
                     LQNQYNKKTENQENGTFHAASSLGLSAVYDFKLNDKFKPYIGARVAYGHVRHSIDSTK
                     KTTGFLTTAGARGAGSAVRPSYKSTQDAHHQSNSIRRV"
BASE COUNT          159 a          177 c          141 g          117 t
ORIGIN      
        1 tcttctcttc tcttctcttc tcttctcttc cgcagcgcgg gcggcaagtg aagacggcgg
       61 ccgcggcccg tatgtgcagg cggatttagc ctacgccgcc gaacgcatta cccacgatta
      121 tccggaacca gccggtgcaa aaaaaggcaa aataagcacg gtaagcgatt atttcagaaa
      181 catccgtacg cattccgtcc accccagggt gtcggtcggc tacgacttcg gcggctggag
      241 gatagcggca gattatgccc gttacagaaa gtggaacaac agtaaatatt ccgtcagcat
      301 aaaaaagttg caaaaccagt ataacaagaa gacggaaaat caggaaaacg gtacgttcca
      361 cgccgcctcc tcgctcggct tgtccgccgt ttacgacttc aaactcaacg acaaattcaa
      421 accctatatc ggtgcgcgcg tcgcctacgg acacgtcaga cacagcatcg attcgaccaa
      481 aaaaacaaca gggtttctta ccaccgccgg tgctcgtggc gcagggtctg cggttcgtcc
      541 ttcttacaaa agtacgcaag acgcccatca ccaaagcaac agcatccgcc gcgt
//
LOCUS       DAWTHD010000210          584 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWTHD010000210 DAWTHD010000000
VERSION     DAWTHD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.211"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            41..256
                     /locus_tag="WM704_002175"
                     /pseudo
     CDS             41..256
                     /locus_tag="WM704_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          182 a          115 c          109 g          178 t
ORIGIN      
        1 ttaacaaaaa ttaatgccct ttcttaggga cgcaatatat aaggtttata ccgtcgttgt
       61 ccctaatgcg caatcagcga cattgttgcc aattatccga aagaaagtta agcctgatgg
      121 cattgtatac acggatacct ttcgtagtta tgatgtgctt gatgttagtg aatttagcca
      181 tttacgtaag tttaacggca ttcccaaaga gcatttgggg ctatatttaa agaaatgcca
      241 atggcatttg aaatagaatc tcaaatttag attttaagac aattagttaa tgggaatttg
      301 gtctggttat ctagtacaac cccaaaagtt tttatataaa aacagtttgg taagttctct
      361 tcgtgaagtg cttatgtttt gccacaaaag ttccataatc atggcggcat cacccaagct
      421 ccatccttcc aagaaaatgg catctttgtt gtggttgaaa atcgactcca tacgattttg
      481 gacaagcata gggatagatg ttctgaaaaa cggcagcgcg ggcattctcc aaccaccttc
      541 ccaaatctta taatacaaac ccgaagacct ttgcaaaatt cccc
//
LOCUS       DAWTHD010000211          568 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 53586
            SAMN23566113-rid13878023.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWTHD010000211 DAWTHD010000000
VERSION     DAWTHD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566113
            Sequence Read Archive: SRR17102448
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241640.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2022 23:21:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,176
            CDSs (total)                      :: 2,130
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 100 of 165
            Pseudo Genes (incomplete)         :: 70 of 165
            Pseudo Genes (internal stop)      :: 37 of 165
            Pseudo Genes (multiple problems)  :: 41 of 165
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566113-rid13878023.denovo.212"
                     /isolate="53586"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-14994"
     gene            <1..447
                     /locus_tag="WM704_002176"
     CDS             <1..447
                     /locus_tag="WM704_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9341498.1"
                     /translation="ATAQTALAVAEAAGTVWRGKKVELNPTKWDWVKNTGYKKPAARH
                     MQTLDGEMAGGNKPVVKSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKID
                     IRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
BASE COUNT          201 a          103 c          124 g          140 t
ORIGIN      
        1 gcaacagccc aaactgccct tgccgtagca gaggccgcag gtacggtttg gagaggtaaa
       61 aaagtagaac ttaacccgac taaatgggat tgggttaaaa ataccggcta taaaaaacct
      121 gctgcccgcc atatgcagac tttagatggg gagatggcag gaggaaacaa gccagttgtt
      181 aaatctatta gaccaactac gcgagatgaa ttacgtcaag cattgcaaga acaaggtttt
      241 agacgtactg gttcagatgc ggctcaatat gaaacatgga aaggtcctga tggcgtgaaa
      301 atagatattc gtccaaatgg agaggttata agaacccaaa gagtgccgcg aaccgatggt
      361 gtacagggaa aatatccgca acgacaagat tatgaaggca atccattgcc aaataatcat
      421 catcattctg gatattttgt caaatgaaaa aaaatatttt tcacaatgta agcctttatg
      481 aaataatctt ttccgataat ggaaataccc ttacattatc ttttacagat acaattgaag
      541 gtaattattt cggatatatc aaatgcag
//
